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Masayuki Maki

Section

The Center for Academic Resources and Archives

Job title

Professor

Degree

博士（理学）（東京大学）

researchmap

https://researchmap.jp/read0191577

J-GLOBAL ID

200901095321698254

e-Rad No.

60263985

Research History 7

2012/04 - Present

Tohoku University　The Center for Academic Resources and Archives, Botanical Gardens
2001/04 - 2012/03

東北大学大学院生命科学研究科　助教授
1998/11 - 2001/03

東北大学大学院理学研究科　助教授
1995/04 - 1998/10

Fukuoka University of Education, Associate Professor　助教授
1994/04 - 1995/03

Fukuoka University of Education, Lecturer　講師
1993/04 - 1994/03

東京大学大学院理学系研究科　学術振興会特別研究員
1992/04 - 1993/03

東京大学大学院理学系研究科　研究生

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Education 2

The University of Tokyo　Graduate School of Sciences　植物学専攻

- 1992/03
The University of Tokyo　Faculty of Agriculture　林学科

- 1987/04

Committee Memberships 7

Scientific Reports, Editorial Board Member

2023/01 - Present
個体群生態学会英文誌Population Ecology; Associate Editor

2021/01 - Present
日本植物分類学会英文誌Acta Phytotaxonomica et Geobotanica; Editor

2017/01 - Present
個体群生態学会英文誌Population Ecology; Editorial Board

2007/01 - 2020/12
日本植物学会英文誌Journal of Plant Research; Editor

2007/01 - 2010/12
日本生態学会英文誌Ecological Research; Board of Editors

2001/01 - 2003/12
日本植物学会英文誌Journal of Plant Research; Editorial Board

1997/01 - 2000/12

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Professional Memberships 4

種生物学会
日本生態学会
日本植物分類学会
日本植物学会

Research Interests 5

系統分類
種分化
集団遺伝
系統進化
生物多様性

Research Areas 3

Environmental science/Agricultural science / Biological resource conservation /
Life sciences / Ecology and environmental science /
Life sciences / Biodiversity and systematics /

Awards 5

Plant Species Biology Best Paper Award 2022

2023/01
日本植物分類学会論文賞

2022/03　日本植物分類学会
日本植物分類学会論文賞

2013/03　日本植物分類学会
日本植物分類学会論文賞

2008/03　日本植物分類学会
日本植物学会奨励賞

2001/09　財団法人日本植物学会　日本産稀少野生植物の集団遺伝学

Papers 164

New Species of Thalictrum (Ranunculaceae) from Okinawa Island in Japan and Its Phylogenetic Implications

KANAE MICHIMOTO, MASATSUGU YOKOTA, ATSUSHI ABE, SHINJI FUJII, DAIKI TAKAHASHI, SATOSHI KAKISHIMA, CHIH CHIEH YU, TAKURO ITO, MASAYUKI MAKI

Phytotaxa　696　(1)　55-70　2025/03/28
Publisher: Magnolia Press
DOI： 10.11646/phytotaxa.696.1.3 　

ISSN： 1179-3155

eISSN： 1179-3163

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This study aims to compare Thalictrum (Ranunculaceae) plant species, tentatively identified as T. minus, from the Yambaru region of Okinawa Island, Japan, with closely related species using morphometric and molecular phylogenetic analyses. Morphological observations revealed that the unique plants from Okinawa Island had clavate filaments and thickened roots. These traits differed significantly from those of typical T. minus, which have filamentous filaments and slender roots. Molecular phylogenetic analyses revealed that the unique plant on Okinawa Island is sister to T. urbaini, endemic to Taiwan, and formed a separate clade from those containing T. minus. Based on current morphological comparisons and molecular phylogenetic analyses, the unique plant on Okinawa Island was concluded to be a previously undescribed species, newly named Thalictrum yambaruense. Furthermore, our molecular phylogenetic analyses of Thalictrum in Japan and Taiwan revealed that T. tuberiferum var. yakusimense, endemic to Yakushima Island, Japan, is phylogenetically distinct from T. tuberiferum var. tuberiferum. Therefore, we propose elevating the taxonomic status of T. tuberiferum var. yakusimense to the species level as T. yakusimense.
Unraveling enigmatic disjunctions: Population genetic analysis points to independent origins of rare rhododendrons in the Rhododendron keiskei complex (Ericaceae) Peer-reviewed

Shota Sakaguchi, Hee‐Joo Choi, Watanabe Yoichi, Daiki Takahashi, Shun K. Hirota, Masayuki Maki, Shoki Murakami, Taichi Harada, Nobuo Kobayashi, Yuji Kurashige, Jun‐Ho Song, Hyeok‐Jae Choi, Seung‐Chul Kim

TAXON　74　in press　2024/11/30
Publisher: Wiley
DOI： 10.1002/tax.13288 　

ISSN： 0040-0262

eISSN： 1996-8175

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Abstract Unraveling species boundaries is pivotal for evolutionary biology and conservation endeavors. However, it proves challenging in instances where recent speciation is intertwined with complex demographic histories and natural selection processes. The Rhododendron keiskei complex, an evergreen rhododendron distributed in East Asia, consists of a widespread variety (R. keiskei var. keiskei) and a more restricted R. keiskei var. hypoglaucum. Intriguingly, the latter is exceptionally rare yet displays a disjunction that spans approximately 1100 km. This study aimed to elucidate the evolutionary backgrounds of the enigmatic disjunctions of R. keiskei var. hypoglaucum and to propose species delimitation within the species complex. An integrative approach, combining genomic data (MIG‐seq and GBS‐derived SNPs) with Scanning Electron Microscopy analysis of leaf microstructures was adopted in this study. Phylogenetic analyses revealed significant divergence among the studied rhododendrons. Genetic demographic analyses favored the population models that assumed non‐monophyly of two disjunct populations of R. keiskei var. hypoglaucum indicating their independent origins. Recent gene flow between the widespread R. keiskei var. keiskei and “var. hypoglaucum” populations were limited due to geographic and habitat isolation factors, even in areas where their distributions overlap. Detailed morphological assessments detected distinctions between morphologically similar “var. hypoglaucum” populations based on leaf microstructures and flowering habits. Our study has shown that the apparent disjunctions of rare rhododendrons are more likely attributed to morphological convergence, possibly due to similar environmental selections in unrelated taxa. The finding highlights the importance of an integrative approach for resolving taxonomic challenges in plant species complexes.
Genetic structure of interspecific hybridization between the long corolla tube species, Isodon longitubus, and its short corolla tube congener, I. inflexus Peer-reviewed

Tadashi Yamashiro, Asuka Yamashiro, Ikumi Dohzono, Masayuki Maki

Plant Systematics and Evolution　310　(6)　41　2024/10/11
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s00606-024-01921-2 　

ISSN： 0378-2697

eISSN： 1615-6110
Inferring complex evolutionary history of the closely related East Asian wild roses in Rosa sect. Synstylae (Rosaceae) based on genomic evidence from conserved orthologues Peer-reviewed

Ji-Hyeon Jeon, Masayuki Maki, Yu-Chung Chiang, Seung-Chul Kim

Annals of Botany　135　in press　2024/09/18
Publisher: Oxford University Press (OUP)
DOI： 10.1093/aob/mcae170 　

ISSN： 0305-7364

eISSN： 1095-8290

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Abstract Background and Aims The section Synstylae in the genus Rosa (Rosaceae) comprises 25–36 species and includes several major progenitors of modern rose cultivars. East Asian Synstylae species have recently diverged and are closely related, but their phylogenetic relationships remain unclear. In the present study, we employed conserved orthologue set (COS) markers and genome-wide nuclear orthologues to elucidate their phylogenetic relationships and unravel their complex evolutionary history. Methods Utilizing eight Rosaceae COS (RosCOS) markers, we analysed a total of 137 accessions representing 15 East Asian Synstylae taxa to establish a robust phylogenetic framework and reconstruct ancestral areas. Furthermore, we constructed the species tree for eight representative species and estimated their divergence times based on 1683 genome-wide orthologues. The species tree–gene tree coalescence time comparison, Patterson’s D, f4-ratio and f-branch statistics were analysed to identify incomplete lineage sorting (ILS), genetic introgression and reticulation events using conserved orthologue data. Key Results RosCOS markers and genome-wide orthologues effectively resolved a robust phylogeny of East Asian Rosa sect. Synstylae. Species divergence times estimated with genome-wide orthologues indicated that East Asian Synstylae species have recently diverged, with an estimated crown age of ~2 Mya. The rampant gene tree discordance indicated the possibility of ILS and/or genetic introgression. In the section Synstylae, deeper coalescence in the gene trees compared to the species tree suggested ILS as a source of gene tree discordance. Further, Patterson’s D and f-branch statistics indicated that several lineages in the section were involved in genetic introgression. Conclusions We have unravelled the complex evolutionary history of East Asian Rosa sect. Synstylae, including recent species divergences, ILS and genetic introgression. Coupled with the geographical and ecological complexity of East Asia, ILS and genetic introgression may have contributed to the rapid diversification of East Asian Synstylae species by permitting adaptation to diverse environments.
Plastid phylogenomics with broad taxon sampling provides insights into the generic delimitation of Paraphlomideae (Lamiaceae) Peer-reviewed

Jing‐Chen Yuan, Ang Liu, Atsuko Takano, Masayuki Maki, Richard G.J. Hodel, Ya‐Ping Chen, Chun‐Lei Xiang

TAXON　73　(4)　1016-1029　2024/08/27
Publisher: Wiley
DOI： 10.1002/tax.13222 　

ISSN： 0040-0262

eISSN： 1996-8175

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Abstract Paraphlomideae (Lamioideae, Lamiaceae) is a recently established tribe endemic to East and Southeast Asia. It comprises three herbaceous genera: Paraphlomis, Matsumurella, and Ajugoides. Phylogenetic relationships among these genera have not yet been satisfactorily resolved and the monophyly of Paraphlomis was challenged in previous molecular phylogenetic studies. In this study, we performed maximum likelihood and Bayesian inference analyses on complete plastomes and nuclear ribosomal internal and external transcribed spacer sequences to further resolve the generic relationships within Paraphlomideae. All phylogenetic analyses support the monophyly of Paraphlomideae. Contrary to traditional classifications, both Ajugoides and Matsumurella were deeply nested within Paraphlomis, indicating the need to expand Paraphlomis to include Ajugoides and Matsumurella. Hence, six new combinations and one replacement name are proposed. Morphologically, species of the newly defined Paraphlomis share a rhizomatous or stoloniferous habit, plants with simple hairs, nutlets with a truncate apex, and actinomorphic calyces. In addition, widespread incongruence between nuclear and plastid trees may have been caused by hybridization and incomplete lineage sorting after rapid diversification in the re‐circumscribed Paraphlomis.
Phylogenetic relationships and divergence time of Hydrangea sect. Macrophyllae (Hydrangeaceae) revealed by genome-wide SNPs Peer-reviewed

Shoki Murakami, Takuro Ito, Tatsuya Uemachi, Shinji Fujii, Ayumi Matsuo, Yoshihisa Suyama, Masayuki Maki

Plant Systematics and Evolution　310　(4)　2024/07/04
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s00606-024-01902-5 　

ISSN： 0378-2697

eISSN： 1615-6110

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Abstract Hydrangea sect. Macrophyllae comprises deciduous shrubs including two species with seven infraspecific taxa distributed throughout the Japanese Archipelago and adjacent areas. In this study, we revealed a phylogeny of Hydrangea sect. Macrophyllae by using a larger dataset of genome-wide SNPs than those employed in a previous study and by adding more comprehensive taxa/populations, which cover the entire distribution of the section. We then revised the taxonomic treatment of sect. Macrophyllae based on the higher-resolution phylogenetic trees obtained in this study. The phylogenetic trees estimated in this study showed five major clades, suggesting that H. serrata var. minamitanii and var. yesoensis should be treated as independent species from other H. serrata, that H. serrata var. australis and var. yakushimensis could be subclassified under the major clades and that var. angustata could be a synonym of var. serrata. The divergence time of these major clades was estimated to be in the Pleistocene.
Genome-wide SNPs reveal clonality and population genetic structure of Nymphoides peltata in Japan (Menyanthaceae) Peer-reviewed

Akira Asakawa, Shoki Murakami, Sachiko Horie, Ayumi Matsuo, Yoshihisa Suyama, Shinji Fujii, Masayuki Maki

Aquatic Botany　190　103720-103720　2024/01
Publisher: Elsevier BV
DOI： 10.1016/j.aquabot.2023.103720 　

ISSN： 0304-3770
Long-styled Variant Decreases Ovule Loss by Hybridization with Short-styled Congener in Oxalis Peer-reviewed

Yusuke Hoshino, Masayuki, Makii, Ikumi Dohzono

Acta Phytotaxonomica et Geobotanica　74　(3)　171-175　2023/11

ISSN： 1346-7565
Development and characterization of expressed sequence tag-simple sequence repeat markers for <i>Anaphalis margaritacea</i> var. <i>yedoensis</i> (Asteraceae) Peer-reviewed

Taishi Hoson, Shoki Murakami, Takuro Ito, Masuyuki Maki

Genes and Genetic Systems　98　(5)　259-265　2023/10/01
Publisher: Genetics Society of Japan
DOI： 10.1266/ggs.23-00037 　

ISSN： 1341-7568

eISSN： 1880-5779
Potential contribution of floral thermogenesis to cold adaptation, distribution pattern, and population structure of thermogenic and non/slightly thermogenic Symplocarpus species Peer-reviewed

Mitsuhiko P. Sato, Ayumi Matsuo, Koichi Otsuka, Kohei Takenaka Takano, Masayuki Maki, Kunihiro Okano, Yoshihisa Suyama, Yasuko Ito‐Inaba

Ecology and Evolution　13　2023/07/15
Publisher: Wiley
DOI： 10.1002/ece3.10319 　

ISSN： 2045-7758

eISSN： 2045-7758
Plastome-based backbone phylogeny of East Asian Phedimus (Subgenus Aizoon: Crassulaceae), with special emphasis on Korean endemics Peer-reviewed

Yongsung Kim, Seon-Hee Kim, JiYoung Yang, Myong-Suk Cho, Marina Koldaeva, Takuro Ito, Masayuki Maki, Seung-Chul Kim

Frontiers in Plant Science　14　2023/03/14
Publisher: Frontiers Media SA
DOI： 10.3389/fpls.2023.1089165 　

eISSN： 1664-462X

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Although the monophyly of Phedimus has been strongly demonstrated, the species relationships among approximately 20 species of Phedimus have been difficult to determine because of the uniformity of their floral characteristics and extreme variation of their vegetative characters, often accompanied by high polyploid and aneuploid series and diverse habitats. In this study, we assembled 15 complete chloroplast genomes of Phedimus species from East Asia and generated a plastome-based backbone phylogeny of the subgenus Aizoon. As a proxy for nuclear phylogeny, we reconstructed the nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) phylogeny independently. The 15 plastomes of subg. Aizoon were highly conserved in structure and organization; hence, the complete plastome phylogeny fully resolved the species relationships with strong support. We found that P. aizoon and P. kamtschaticus were polyphyletic and morphologically distinct or ambiguous species, and they most likely evolved from the two species complex. The crown age of subg. Aizoon was estimated to be 27 Ma, suggesting its origin to be in the late Oligocene; however, the major lineages were diversified during the Miocene. The two Korean endemics, P. takesimensis and P. zokuriensis, were inferred to have originated recently during the Pleistocene, whereas the other endemic, P. latiovalifolium, originated in the late Miocene. Several mutation hotspots and seven positively selected chloroplast genes were identified in the subg. Aizoon.
The effects of inbreeding depression and pollinator visitation on the maintenance of herkogamy in Oxalis corniculata , a species derived from a heterostylous ancestor Peer-reviewed

Yusuke Hoshino, Minori Hoshino, Kazuki Yoshioka, Tsubasa Washio, Makoto Nakamura, Masayuki Maki, Ikumi Dohzono

Plant Species Biology　37　(6)　349-360　2022/11
Publisher: Wiley
DOI： 10.1111/1442-1984.12387 　

ISSN： 0913-557X

eISSN： 1442-1984
The Genetic Diversity and Structure of the Feral Raccoon (Procyon lotor) Population in Shikoku Island, Japan Peer-reviewed

Tadashi Yamashiro, Yoshinori Kaneshiro, Asuka Yamashiro, Masayuki Maki

Mammal Study　47　(3)　2022/06/09
Publisher: Mammalogical Society of Japan
DOI： 10.3106/ms2021-0052 　

ISSN： 1343-4152
The complete chloroplast genome sequence of Thalictrum aquilegiifolium var. sibiricum (Ranunculaceae) Peer-reviewed

Kanae Michimoto, Takuro Ito, Masayuki Maki

Mitochondrial DNA Part B　7　(6)　1171-1173　2022/06/03
Publisher: Informa UK Limited
DOI： 10.1080/23802359.2022.2088309 　

eISSN： 2380-2359
Secondary contact and adaptation to local pollinator assemblages mediate geographical variation in corolla length in <scp>Isodon</scp> shikokianus Peer-reviewed

Miho Ogishima, Yusuke Hoshino, Sachiko Horie, Tadashi Yamashiro, Masayuki Maki, Kazuo Suzuki, Ikumi Dohzono

Plant Species Biology　37　(3)　222-230　2022/05
Publisher: Wiley
DOI： 10.1111/1442-1984.12370 　

ISSN： 0913-557X

eISSN： 1442-1984
The complete chloroplast genome sequence of Anaphalis margaritacea var. yedoensis (Asteraceae) and phylogenetic relationships within Gnaphalieae Peer-reviewed

Taishi Hoson, Takuro Ito, Masayuki Maki

Mitochondrial DNA Part B　7　(3)　563-565　2022/03/04
Publisher: Informa {UK} Limited
DOI： 10.1080/23802359.2022.2048213 　
Can Rumex madaio (Polygonaceae) be threatened by natural hybridization with an invasive species in Japan Peer-reviewed

Ryudo Uemura, Akira Asakawa, Shinji Fujii, Ayumi Matsuo, Yoshihisa Suyama, Masayuki Maki

Nordic Journal of Botany　2022/03/03
Publisher: Wiley
DOI： 10.1111/njb.03543 　

ISSN： 0107-055X

eISSN： 1756-1051
Multiple colonizations and genetic differentiation in goldenrod populations on recently formed nearshore islands Peer-reviewed

Takuma Kimura, Takayuki Yamada, Shota Sakaguchi, Motomi Ito, Masayuki Maki

Journal of Biogeography　49　(5)　836-852　2022/03
Publisher: Wiley
DOI： 10.1111/jbi.14342 　

ISSN： 0305-0270

eISSN： 1365-2699

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Abstract Aim Although the evolution of island endemic plants has long been investigated, the majority of such studies have focused on species with remarkable levels of morphological variation and on islands substantially far from the mainland. Endemic plants on nearshore oceanic islands have received less attention. We investigate the population genetic structure and dynamics in plants endemic to nearshore and recently formed oceanic islands and examined the possibility of multiple colonizations onto the islands. Location Japanese mainland Honshu and the adjacent Izu Islands. Taxon Solidago virgaurea (Asteraceae). Methods Sixteen and nine populations of S. virgaurea complex were sampled from the mainland and islands respectively; phylogeographical and population genetics analyses, including Bayesian Phylogeographic and Ecological Clustering (BPEC) analysis and Discrete Phylogeographic Approach (DPA) to trace the history of colonization events onto the islands, were performed using plastid DNA and nuclear microsatellite DNA variations. Results Phylogenetically close plastid DNA haplotypes were shared between the mainland and islands, although the populations of S. virgaurea from different islands tended to exhibit phylogenetically distinct haplotypes. Admixture analyses based on nuclear DNA variations revealed distinct genetic structures between the mainland and island populations. Gene flow among islands is restricted but may partially offset genetic drift on each island. Main conclusions The genetic structure observed in this study may not have originated from a single dispersal event and successive expansion but rather from at least three colonization events and subsequent gene flow among island populations. Based on the nuclear DNA variations, the Izu Island populations of S. virgaurea are genetically distinct from the mainland ones. Repeated colonization events may have provided sufficient genetic diversity, which would generally be susceptible to founder effects and exert a driving force for evolutionary adaptation, to these oceanic island populations.
Plastome characterization and comparative analyses of wild crabapples (Malus baccata and M. toringo): insights into infraspecific plastome variation and phylogenetic relationships Peer-reviewed

Myong-Suk Cho, Jin Hyeong Kim, Takayuki Yamada, Masayuki Maki, Seung-Chul Kim

Tree Genetics & Genomes　17　(5)　2021/10
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s11295-021-01520-z 　

ISSN： 1614-2942

eISSN： 1614-2950
Population genetic diversity and conservation priority of prince’s pine Chimaphila umbellata populations around the south margin of their distribution Peer-reviewed

Ai Kikuchi, Ryuta Kyan, Masayuki Maki

Conservation Genetics　22　(5)　839-853　2021/10
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s10592-021-01366-x 　

ISSN： 1566-0621

eISSN： 1572-9737
Genome-Wide Single Nucleotide Polymorphism Analysis Elucidates the Evolution of Prunus takesimensis in Ulleung Island: The Genetic Consequences of Anagenetic Speciation Peer-reviewed

Myong-Suk Cho, Koji Takayama, JiYoung Yang, Masayuki Maki, Seung-Chul Kim

Frontiers in Plant Science　12　706195　2021/09/02
Publisher: Frontiers Media SA
DOI： 10.3389/fpls.2021.706195 　

eISSN： 1664-462X
Development of microsatellite markers for Meliosma arnottiana (Sabiaceae) disjunctively distributed in the Japanese archipelago Peer-reviewed

Shoki Murakami, Takuro Ito, Akira Asakawa, Shinji Fujii, Masayuki Maki

Journal of Forest Research　1-4　2021/06/22
Publisher: Informa UK Limited
DOI： 10.1080/13416979.2021.1941561 　

ISSN： 1341-6979

eISSN： 1610-7403
Species cohesion of an extremophyte (Carex angustisquama, Cyperaceae) in solfatara fields maintained under interspecific natural hybridization Peer-reviewed

Koki Nagasawa, Hiroaki Setoguchi, Masayuki Maki, Kazuhiro Sawa, Kenji Horie, Shota Sakaguchi

Annals of Botany　128　(3)　343-356　2021/06/09
Publisher: Oxford University Press (OUP)
DOI： 10.1093/aob/mcab069 　

ISSN： 0305-7364

eISSN： 1095-8290

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<title>Abstract</title> <sec> <title>Background and Aims</title> Hybridization is the main driver of plant diversification, and gene flow via hybridization has multifaceted effects on plant evolution. Carex angustisquama is an extremophyte that grows on soils heavily acidified by volcanism. Despite its distinct habitat from other species, this species is known to form interspecific hybrids, implying interspecific gene flow. It is crucial to verify the extent and direction of interspecific gene flow between C. angustisquama and closely related species to understand the evolutionary process of an extremophyte in solfatara fields. </sec> <sec> <title>Methods</title> In this study, expressed sequence tag-simple sequence repeat markers were utilized to infer the extent and direction of interspecific gene flow between C. angustisquama and closely related species. </sec> <sec> <title>Key Results</title> Bayesian clustering and simulation analyses revealed that all individuals of the three hybrid species were classified into the first hybrid generation or first backcross to C. angustisquama; therefore, current interspecific gene flow is limited. Moreover, in the Bayesian inference of historical gene flow based on multispecies samples, the model that assumed no interspecific gene flow was the most strongly supported across all species pairs, including phylogenetically close but ecologically distinctive species pairs. </sec> <sec> <title>Conclusions</title> Our results revealed that interspecific gene flow between C. angustisquama and its related species has been limited both currently and historically. Moreover, our results of Bayesian inference of historical gene flow indicated that extrinsic, rather than intrinsic, factors likely act as isolating barriers between Carex species, with hybrid breakdown via microhabitat segregation being the probable potential barrier. Overall, our findings provide insights into the evolutionary process of an extremophyte in solfatara fields and offer an important example of the mechanisms of diversification of the speciose genus Carex. </sec>
Refugia during the last glacial period and the origin of the disjunct distribution of an insular plant Peer-reviewed

Takayuki Yamada, Goro Kokubugata, Shinji Fujii, Chien Fan Chen, Akira Asakawa, Takuro Ito, Masayuki Maki

Journal of Biogeography　48　(6)　1460-1474　2021/06

DOI： 10.1111/jbi.14090 　

ISSN： 0305-0270

eISSN： 1365-2699
Phylogeographic and demographic modeling analyses of the multiple origins of the rheophytic goldenrod Solidago yokusaiana Makino Peer-reviewed

Ryuuta Kyan, Takuma Kimura, Tadashi Yamashiro, Shinji Fujii, Shota Sakaguchi, Motomi Ito, Atsushi J. Nagano, Hiroshi Kudoh, Masayuki Maki

Heredity　126　(5)　831-845　2021/05

DOI： 10.1038/s41437-021-00408-x 　

ISSN： 0018-067X

eISSN： 1365-2540
Assessing insect herbivory on broadleaf canopy trees at 19 natural forest sites across Japan Peer-reviewed

Hino Takafumi, Yuri Kanno, Shin Abe, Tetsuto Abe, Tsutomu Enoki, Toshihide Hirao, Tsutom Hiura, Kazuhiko Hoshizaki, Hideyuki Ida, Ken Ishida, Masayuki Maki, Takashi Masaki, Shoji Naoe, Mahoko Noguchi, Tatsuya Otani, Takanori Sato, Michinori Sakimoto, Hitoshi Sakio, Masahiro Takagi, Atsushi Takashima, Naoko Tokuchi, Shunsuke Utsumi, Amane Hidaka, Masahiro Nakamura

Ecological Research　36　(3)　562-572　2021/05

DOI： 10.1111/1440-1703.12215 　

ISSN： 0912-3814

eISSN： 1440-1703
Symplocarpus koreanus (Araceae; Orontioideae), a new species based on morphological and molecular data Peer-reviewed

Joon Seon LEE, Seon-Hee KIM, Yongsung KIM, Youl KWON, JiYoung YANG, Myong-Suk CHO, Hye-Been KIM, Sangryong LEE, Masayuki MAKI, Seung-Chul KIM

Korean Journal of Plant Taxonomy　51　(1)　1-9　2021/03/31
Publisher: The Korean Society of Plant Taxonomists
DOI： 10.11110/kjpt.2021.51.1.1 　

ISSN： 1225-8318

eISSN： 2466-1546
Dauciform roots in Aletris spicate (Thunb.) Franch. (Nartheciaceae). Peer-reviewed

Shinji Fujii, Masayuki Maki

Journal of Japanese Botany　96　(5)　294-296　2021
Two new natural hybrids between Carex angustisquama (Cyperaceae) and its related species from solfatara fields in Tohoku region, Japan. Peer-reviewed

Koki Nagasawa, Kazuhiro Sawa, Hiroaki Setoguchi, Masayuki Maki, Shota Sakatuchi

Acta Phytotaxonomica et Geobotanca　72　(3)　241-251　2021
Genetic structure of a rare interspecific hybridization of Vincetoxicum atratum and V. pyconostelima (Apocynaceae: Asclepiadaceae). Peer-reviewed

Tadashi Yamashiro, Asuka Yamashiro, Michiyuki Matuoka, Tomiki Kobayashi, Masayuki Maki

Acta Phytotaxonomica et Geobotanca　72　(3)　227-239　2021
A new species, Petrosavia amanuensis (Petrosaviaceae), from Amami-Oshima Islands, Kagoshima Prefecture, Japan. Peer-reviewed

Hiroshi Takahashi, Tomohisa Yukawa, Masayuki Maki

Acta Phytotaxonomica et Geobotanca　72　(3)　265-274　2021
Functional traits divergence in parallelly evolved rheophytic populations of solidago virgaurea l. Complex (asteraceae) in japan Peer-reviewed

Shota Sakaguchi, Atsushi Abe, Koki Nagasawa, Daiki Takahashi, Hiroaki Setoguchi, Masayuki Maki, Ryuta Kyan, Takako Nishino, Naoko Ishikawa, Shun K. Hirota, Yoshihisa Suyama, Motomi Ito

Acta Phytotaxonomica et Geobotanica　72　(2)　93-111　2021

DOI： 10.18942/apg.202012 　

ISSN： 1346-7565

eISSN： 2189-7042
Origin of the critically endangered endemic species Scrophularia takesimensis (Scrophulariaceae) on Ulleung Island, Korea: implications for conservation Peer-reviewed

Hee Young Gil, Masayuki Maki, Elena A. Pimenova, Aleksandr Taran, Seung Chul Kim

Journal of Plant Research　133　(6)　765-782　2020/11/01

DOI： 10.1007/s10265-020-01221-z 　

ISSN： 0918-9440

eISSN： 1618-0860
Plastome phylogenomic insights into the Sino-Japanese biogeography of Diabelia (Caprifoliaceae) Peer-reviewed

Hong Xin Wang, Michael J. Moore, Russell L. Barrett, Sven Landrein, Shota Sakaguchi, Masayuki Maki, Jun Wen, Hua Feng Wang

Journal of Systematics and Evolution　58　(6)　972-987　2020/11

DOI： 10.1111/jse.12560 　

ISSN： 1674-4918

eISSN： 1759-6831
Incorporating differences between genetic diversity of trees and herbaceous plants in conservation strategies Peer-reviewed

Mi Yoon Chung, Sungwon Son, Sonia Herrando-Moraira, Cindy Q. Tang, Masayuki Maki, Young Dong Kim, Jordi López-Pujol, James L. Hamrick, Myong Gi Chung

Conservation Biology　34　(5)　1142-1151　2020/10/01

DOI： 10.1111/cobi.13467 　

ISSN： 0888-8892

eISSN： 1523-1739
Genetic consequences of plant edaphic specialization to solfatara fields: Phylogenetic and population genetic analysis of Carex angustisquama (Cyperaceae) International-journal Peer-reviewed

Koki Nagasawa, Hiroaki Setoguchi, Masayuki Maki, Hayato Goto, Keitaro Fukushima, Yuji Isagi, Yoshihisa Suyama, Ayumi Matsuo, Yoshihiro Tsunamoto, Kazuhiro Sawa, Shota Sakaguchi

Molecular Ecology　29　(17)　3234-3247　2020/09/01

DOI： 10.1111/mec.15324 　

ISSN： 0962-1083

eISSN： 1365-294X
Leaf morphology of saxifraga fortunei var. Obtusocuneata and var. suwoensis (saxifragaceae) Peer-reviewed

Shinji Fujii, Yoshinori Tokuoka, Masayuki Maki

Acta Phytotaxonomica et Geobotanica　71　(3)　231-242　2020

DOI： 10.18942/apg.202001 　

ISSN： 1346-7565

eISSN： 2189-7042
New insights into the phylogeny and biogeography of subfamily Orontioideae (Araceae) Peer-reviewed

Joon Seon Lee, Seon‐Hee Kim, Sangryong Lee, Masayuki Maki, Koichi Otsuka, Andrey E. Kozhevnikov, Zoya V. Kozhevnikova, Jun Wen, Seung‐Chul Kim

Journal of Systematics and Evolution　57　(6)　616-632　2019/11
Publisher: Wiley
DOI： 10.1111/jse.12498 　

ISSN： 1674-4918

eISSN： 1759-6831
The complete chloroplast genome, Dechesnea chrysantha (Zoll. & Moritzi) Miq. (Rosoideae). Peer-reviewed

Park, J, Heo, K.-I, Kim, Y, Maki, M, Lee, S

Mitochondrial DNA Part B: Resources　4　951-952　2019
Frequent chloroplast capture among Isodon (Lamiaceae) species in Japan revealed by phylogenies based on variation in chloroplast and nuclear DNA. Peer-reviewed

Ogishima, M, Horie, S, Kimura, M, Yamashiro, T, Dohzono, I, Kawaguchi, L, Nagano, A. J, Maki, M

Plant Species Biology　34　(3)　127-137　2019

DOI： 10.1111/1442-1984.12239 　

ISSN： 0913-557X

eISSN： 1442-1984
The complete chloroplast genome of mock strawberry, Duchesnea indica (Andrews) Th.Wolf (Rosaceae). Peer-reviewed

Heo, K.-I, Kim, Y, Maki, M, Park, J

Mitochondrial DNA Part B: Resources　4　560-562　2019
Hybridization, coexistence and possible reproductive interferance between native Oxalis corniculata and O. dellenii in Japan. Peer-reviewed

Fukatsu, M, Horie, S, Maki, M, Dohozono I

Plant Systematics and Evolution　305　127-137　2019
Crassula peduncularis and C. saginoides (Crassulacaede), newly naturalized plants in Japan, and their genetic differences from C. aquatica. Peer-reviewed

Fujii, S, Yamashiro, T, Horie, S, Maki, M

Acta Phytotaxonomica et Geobotanica　70　119-127　2019
Comparison of whole plastome sequences between thermogenic skunk cabbage Symplocarpus renifolius and nonthermogenic Symplocarpus nipponicus (Orontioideae: Araceae) in East Asia. Peer-reviewed

Kim, S.-H, Yang, J. Y, Park, J, Yamada, T, Maki, M, Kim, S.-C

International Journal of Molecular Sciences　20　4678　2019
Multiple origins and the population genetic structure of Rubus takesimensis (Rosaceae) on Ulleung Island: implications for the genetic consequences of anagenetic speciation. Peer-reviewed

Yang, J. Y, Pak, J.-H, Maki, M, Kim, S.-C

PLoS ONE　14　e0222707　2019
Phylogeographic Analysis and Genetic Structure of an Endemic Sino-Japanese Disjunctive Genus Diabelia (Caprifoliaceae). International-journal Peer-reviewed

Kun-Kun Zhao, Sven Landrein, Russell L Barrett, Shota Sakaguchi, Masayuki Maki, Wei-Xue Mu, Ting Yang, Zhi-Xin Zhu, Huan Liu, Hua-Feng Wang

Frontiers in plant science　10　913-913　2019

DOI： 10.3389/fpls.2019.00913 　

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The Sino-Japanese Floristic Region (SJFR) is a key area for plant phylogeographical research, due to its very high species diversity and disjunct distributions of a large number of species and genera. At present, the root cause and temporal origin of the discontinuous distribution of many plants in the Sino-Japanese flora are still unclear. Diabelia (Caprifoliaceae; Linnaeoideae) is a genus endemic to Asia, mostly in Japan, but two recent discoveries in China raised questions over the role of the East China Sea (ECS) in these species' disjunctions. Chloroplast DNA sequence data were generated from 402 population samples for two regions (rpl32-trnL, and trnH-psbA) and 11 nuclear microsatellite loci were screened for 549 individuals. Haplotype, population-level structure, combined analyses of ecological niche modeling, and reconstruction of ancestral state in phylogenies were also performed. During the Last Glacial Maximum (LGM) period after the Tertiary, Diabelia was potentially widely distributed in southeastern China, the continental shelf of the East China Sea and Japan (excluding Hokkaido). After LGM, all populations in China have disappeared except those in Zhejiang which may represent a Glacial refuge. Populations of Diabelia in Japan have not experienced significant bottleneck effects, and populations have maintained a relatively stable state. The observed discontinuous distribution of Diabelia species between China and Japan are interpreted as the result of relatively ancient divergence. The phylogenetic tree of chloroplast fragments shows the characteristics of multi-origin evolution (except for D. sanguinea). STRUCTURE analysis of nuclear Simple Sequence Repeat (nSSR) showed that the plants of the Diabelia were divided into five gene pools: D. serrata, D. spathulata, D. sanguinea, D. ionostachya (D. spathulata var. spathulata-Korea), and populations of D. ionostachya var. ionostachya in Yamagata prefecture, northern Japan. Molecular evidence provides new insights of Diabelia into biogeography, a potential glacial refuge, and population-level genetic structure within species. In the process of species differentiation, ECS acts as a corridor for two-way migration of animals and plants between China and Japan during glacial maxima, providing the possibility of secondary contact for discontinuously distributed species between China and Japan, or as a filter (creating isolation) during glacial minima. The influence of the ECS in speciation and biogeography of Diabelia in the Tertiary remains unresolved in this study. Understanding origins, evolutionary histories, and speciation will provide a framework for the conservation and cultivation of Diabelia.
Development and characterization of EST-SSR markers for Carex angustisquama (Cyperaceae), an extremophyte in solfatara fields. International-journal Peer-reviewed

Koki Nagasawa, Hiroaki Setoguchi, Masayuki Maki, Hayato Goto, Keitaro Fukushima, Yuji Isagi, Shota Sakaguchi

Applications in plant sciences　6　(10)　e01185　2018/10

DOI： 10.1002/aps3.1185 　

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Premise of the Study: Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed for Carex angustisquama (Cyperaceae) to investigate the evolutionary history of this plant that is endemic to solfatara fields in northern Japan. Methods and Results: Using RNA-Seq data generated by the Illumina HiSeq 2000, 20 EST-SSR markers were developed. Polymorphisms were assessed in C. angustisquama and the closely related species C. doenitzii and C. podogyna. In C. angustisquama, many loci were monomorphic within populations; the average number of alleles ranged from one to five, and levels of expected heterozygosity ranged from 0.000 to 0.580, while all markers were polymorphic in a population of C. doenitzii. This indicates that low genetic polymorphism of C. angustisquama is likely due to the species' population dynamics, rather than to null alleles at the developed markers. Conclusions: These markers will be used to assess genetic diversity and structure and to investigate evolutionary history in future studies of C. angustisquama and related species.
Effect of historical factors on genetic variation in three terrestrial Cephalanthera species (Orchidaceae) with different breeding system on the Korean Peninsula Peer-reviewed

Mi Yoon Chung, Nhan Thien Lu, Jordi Lopez-Pujol, Sonia Herrando-Moraira, Jae Min Chung, Huai Zhen Tian, Kenji Suetsugu, Takayuki Kawahara, Tomohisa Yukawa, Masayuki Maki, Pankaj Kumar, Young-Dong Kim, Myong Gi Chung

NORDIC JOURNAL OF BOTANY　36　(7)　2018/07

DOI： 10.1111/njb.01862 　

ISSN： 0107-055X

eISSN： 1756-1051
Development of microsatellite markers for the endangered semi-shrub Chimaphila umbellata (Ericaceae) Peer-reviewed

Ai Kikuchi, Taisuke Miyazaki, Masayuki Maki

Plant Species Biology　33　(2)　140-143　2018/04/01
Publisher: Blackwell Publishing
DOI： 10.1111/1442-1984.12198 　

ISSN： 1442-1984 0913-557X
Phylogeographic analysis of the East Asian goldenrod (Solidago virgaurea complex, Asteraceae) reveals hidden ecological diversification with recurrent formation of ecotypes. International-journal Peer-reviewed

Shota Sakaguchi, Takuma Kimura, Ryuta Kyan, Masayuki Maki, Takako Nishino, Naoko Ishikawa, Atsushi J Nagano, Mie N Honjo, Masaki Yasugi, Hiroshi Kudoh, Pan Li, Hyeok Jae Choi, Olga A Chernyagina, Motomi Ito

Annals of botany　121　(3)　489-500　2018/03/05

DOI： 10.1093/aob/mcx182 　

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Background and Aims: The processes and mechanisms underlying lineage diversification are major topics in evolutionary biology. Eurasian goldenrod species of the Solidago virgaurea complex show remarkable morphological and ecological diversity in the Japanese Archipelago, with ecotypic taxa well adapted to specific environments (climate, edaphic conditions and disturbance regimes). The species complex is a suitable model to investigate the evolutionary processes of actively speciating plant groups, due to its ability to evolve in relation to environmental adaptation and its historical population dynamics. Methods: Two chloroplast markers, 18 nuclear microsatellite markers and ddRAD-sequencing were used to infer population genetic demography of S. virgaurea complex with its related species/genera. Key Results: Our analysis showed that populations in Japan form an evolutionary unit, which was genetically diverged from adjacent continental populations. The phylogenetic structure within the archipelago strongly corresponds to the geography, but interestingly there is no concordance between genetic structure and ecotypic boundaries; neighbouring populations of distinct ecotypes share a genetic background. Conclusions: We propose that the traits specific to the ecotypic entities are maintained by natural selection or are very recently generated and have little effect on the genomes, making genome-wide genetic markers unsuitable for detecting ecotypic differentiation. Furthermore, some sporadically distributed taxa (found as rheophytes and alpine plants) were repeatedly generated from a more widespread taxon in geographically distant areas by means of selection. Overall, this study showed that the goldenrod complex has a high ability to evolve, enabling rapid ecological diversification over a recent timeframe.
The giant mycoheterotrophic orchid Erythrorchis altissima is associated mainly with a divergent set of wood-decaying fungi Peer-reviewed

Yuki Ogura-Tsujita, Gerhard Gebauer, Hui Xu, Yu Fukasawa, Hidetaka Umata, Kenshi Tetsuka, Miho Kubota, Julienne M.-I. Schweiger, Satoshi Yamashita, Nitaro Maekawa, Masayuki Maki, Shiro Isshiki, Tomohisa Yukawa

Molecular Ecology　27　(5)　1324-1337　2018/03/01
Publisher: Blackwell Publishing Ltd
DOI： 10.1111/mec.14524 　

ISSN： 1365-294X 0962-1083
Fine-scale genetic structure in populations of the spring ephemeral herb Megaleranthis saniculifolia (Ranunculaceae) Peer-reviewed

Mi Yoon Chung, John D. Nason, Jordi López-Pujol, Jae Min Chung, Ki-Joong Kim, Masayuki Maki, Myong Gi Chung

Flora: Morphology, Distribution, Functional Ecology of Plants　240　16-24　2018/03/01
Publisher: Elsevier GmbH
DOI： 10.1016/j.flora.2017.12.006 　

ISSN： 0367-2530
Chromosome number of Microtropis japonica (Celastraceae) in Japan. Peer-reviewed

Fukuda, T, Yamada, T, Maki, M

Journal of Japanese Botany　93　209-211　2018
Natural hybridization between endangered herb Artemisia gilvescens (Asteraceae) and common congener, Artemisia indica var. maximowiczii in Japan. Peer-reviewed

Yamashiro, T, Ogawa, M, Yamashiro, A, Maki, M

Acta Phytotaxonomica et Geobotanica　69　(2)　109-117　2018

DOI： 10.18942/apg.201802 　

ISSN： 1346-7565

eISSN： 2189-7042
Development of microsatellite markers for the wind cave-associated shrub Lonicera alpigena subsp. glehnii (Caprifoliaceae) Peer-reviewed

Taisuke Miyazaki, Takuma Kimura, Kentaro Kamata, Masayuki Maki

Applications in Plant Sciences　6　(1)　e1014　2018/01/01
Publisher: Wiley-Blackwell
DOI： 10.1002/aps3.1014 　

ISSN： 1537-2197 2168-0450
Development and characterization of EST-SSR markers in an East Asian temperate plant genus Diabelia (Caprifoliaceae) Peer-reviewed

Kun-Kun Zhao, Hua-Feng Wang, Shota Sakaguchi, Sven Landrein, Yuji Isagi, Masayuki Maki, Zhi-Xin Zhu

PLANT SPECIES BIOLOGY　32　(3)　247-251　2017/07

DOI： 10.1111/1442-1984.12143 　

ISSN： 0913-557X

eISSN： 1442-1984
Isolation and characterization of microsatellite loci in the endangered perennial Eleocharis parvula (Cyperaceae), emerging in the 2011 tsunami inundation areas Peer-reviewed

Takuma Kimura, Shohei Yamazaki, Kunihiko Uno, Takayuki Yamada, Masayuki Maki

PLANT SPECIES BIOLOGY　32　(2)　169-172　2017/04

DOI： 10.1111/1442-1984.12135 　

ISSN： 0913-557X

eISSN： 1442-1984
Chloroplast DNA Assessment of Anagenetic Speciation in Rubus takesimensis (Rosaceae) on Ulleung Island, Korea Peer-reviewed

Woong Lee, JiYoung Yang, Keum-Sun Jung, Jae-Hong Pak, Masayuki Maki, Seung-Chul Kim

JOURNAL OF PLANT BIOLOGY　60　(2)　163-174　2017/04

DOI： 10.1007/s12374-016-0421-9 　

ISSN： 1226-9239

eISSN： 1867-0725
Microsatellite records for volume 9, issue 1 Peer-reviewed

Ana Castillo-Paez, Axayacatl Rocha-Olivares, Celia Isabel Bisbal-Pardo, Dongfa Zhu, Dongmei Zhu, Hajime Itoh, Hiroaki Fukumori, Joichiro Koseki, John Carlos Garza, Jotaro Urabe, Yefeng Lv, Masayuki Maki, Miguel Angel del Rio-Portilla, Satoshi Chiba, Wataru Makino, Weimin Wang, Wen Song, Xuejun Chai, Yasunori Kano, Yuebin Wang, Zehui Hu

CONSERVATION GENETICS RESOURCES　9　(1)　165-171　2017/03

DOI： 10.1007/s12686-017-0689-1 　

ISSN： 1877-7252

eISSN： 1877-7260
The diversity of mycorrhizal fungi in Japanese Cephalanthera species Peer-reviewed

Yuki Sakamoto, Jun Yamazaki, Takayuki Yamada, Jun Yokoyama, Yuki Ogura-Tsujita, Masayuki Maki

PLANT SPECIES BIOLOGY　32　(1)　81-86　2017/01

DOI： 10.1111/1442-1984.12124 　

ISSN： 0913-557X

eISSN： 1442-1984
PHYLOGENY OF GALIUM L. (RUBIACEAE) FROM KOREA AND JAPAN BASED ON CHLOROPLAST DNA SEQUENCE Peer-reviewed

Keum Seon Jeong, Jae Kwon Shin, Masayuki Maki, Jae-Hong Pak

BANGLADESH JOURNAL OF PLANT TAXONOMY　23　(2)　237-246　2016/12

ISSN： 1028-2092

eISSN： 2224-7297
The tiny-leaved orchid Cephalanthera subaphylla obtains most of its carbon via mycoheterotrophy Peer-reviewed

Yuki Sakamoto, Yuki Ogura-Tsujita, Kinuko Ito, Kenji Suetsugu, Jun Yokoyama, Jun Yamazaki, Tomohisa Yukawa, Masayuki Maki

JOURNAL OF PLANT RESEARCH　129　(6)　1013-1020　2016/11

DOI： 10.1007/s10265-016-0856-6 　

ISSN： 0918-9440

eISSN： 1618-0860
Genetic Diversity and Divergence in Populations of the Threatened Grassland Perennial Vincetoxicum atratum (Apocynaceae-Asclepiadoideae) in Japan Peer-reviewed

Tadashi Yamashiro, Asuka Yamashiro, Masahito Inoue, Masayuki Maki

JOURNAL OF HEREDITY　107　(5)　455-462　2016/09

DOI： 10.1093/jhered/esw034 　

ISSN： 0022-1503

eISSN： 1465-7333
Invasion history of Cardamine hirsuta in Japan inferred from genetic analyses of herbarium specimens and current populations Peer-reviewed

Saeko Matsuhashi, Hiroshi Kudoh, Masayuki Maki, Maria Cartolano, Miltos Tsiantis, Tomoyuki Itagaki, Satoki Sakai

BIOLOGICAL INVASIONS　18　(7)　1939-1951　2016/07

DOI： 10.1007/s10530-016-1139-9 　

ISSN： 1387-3547

eISSN： 1573-1464
Differential self-fertilization rates in response to variation in floral traits within inflorescences of Aquilegia buergeriana var. oxysepala (Ranunculaceae) Peer-reviewed

T. Itagaki, M. K. Kimura, M. Maki, S. Sakai

BOTANICAL JOURNAL OF THE LINNEAN SOCIETY　181　(2)　294-304　2016/06

DOI： 10.1111/boj.12410 　

ISSN： 0024-4074

eISSN： 1095-8339
Characterization of 22 polymorphic microsatellite loci for Cryptoblepharus nigropunctatus. Peer-reviewed

Koseki J, Makino W, Maki M, Urabe J, Chiba S

Conservation Genetics Resources　9　(1)　2016
Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of secondary contact between two Vincetoxicum species (Apocynaceae-Asclepiadoideae) Peer-reviewed

Yue Li, Fumito Tada, Tadashi Yamashiro, Masayuki Maki

BMC EVOLUTIONARY BIOLOGY　16　20　2016/01

DOI： 10.1186/s12862-016-0587-2 　

ISSN： 1471-2148
Phylogeography of Arabis serrata (Brassicaceae) in the Japanese Archipelago based on chloroplast DNA variations Peer-reviewed

Ayumi Kizawa, Masayuki Maki

PLANT SPECIES BIOLOGY　31　(1)　65-72　2016/01

DOI： 10.1111/1442-1984.12072 　

ISSN： 0913-557X

eISSN： 1442-1984
Molecular identification of alien species of Vallisneria (Hydrocharitaceae) species in Japan with a special emphasis on the commercially traded accessions and the discovery of hybrid between nonindigenous V. spiralis and native V. denseserrulata Peer-reviewed

Hayato Wasekura, Sachiko Horie, Shinji Fujii, Masayuki Maki

AQUATIC BOTANY　128　1-6　2016/01

DOI： 10.1016/j.aquabot.2015.09.002 　

ISSN： 0304-3770

eISSN： 1879-1522
Origins of Hosta Cultivars Based on Sequence Variations in Chloroplast DNA Peer-reviewed

Sangryong Lee, Masayuki Maki

HORTICULTURE JOURNAL　84　(4)　350-354　2015/10

DOI： 10.2503/hortj.MI-040 　

ISSN： 2189-0102

eISSN： 2189-0110
DEVELOPMENT AND CHARACTERIZATION OF MICROSATELLITE MARKERS FOR VERATRUM MAACKII (MELANTHIACEAE) Peer-reviewed

Yue Li, Satoshi Iijima, Masayuki Maki

APPLICATIONS IN PLANT SCIENCES　3　(6)　1500030　2015/06

DOI： 10.3732/apps.1500030 　

ISSN： 2168-0450
Variation in the Frequency and Extent of Hybridization between Leucosceptrum japonicum and L. stellipilum (Lamiaceae) in the Central Japanese Mainland Peer-reviewed

Yue Li, Masayuki Maki

PLOS ONE　10　(3)　e0116411　2015/03

DOI： 10.1371/journal.pone.0116411 　

ISSN： 1932-6203
Morphological and genetic variation in populations in a hybrid zone between Leucosceptrum japonicum and L-stellipilum (Lamiaceae) in the central Japanese mainland Peer-reviewed

Yue Li, Tomomi Itoi, Hiroshi Takahashi, Masayuki Maki

PLANT SYSTEMATICS AND EVOLUTION　301　(3)　1029-1041　2015/03

DOI： 10.1007/s00606-014-1134-5 　

ISSN： 0378-2697

eISSN： 1615-6110
Mycorrhizal diversity of the orchid Cephalanthera longibracteata in Japan Peer-reviewed

Yuki Sakamoto, Jun Yokoyama, Masayuki Maki

MYCOSCIENCE　56　(2)　183-189　2015/03

DOI： 10.1016/j.myc.2014.06.002 　

ISSN： 1340-3540

eISSN： 1618-2545
Relationships between floral morphology and pollinator fauna in insular and main island populations of Hosta longipes ( Liliaceae) Peer-reviewed

Takayuki Yamada, Masayuki Maki

PLANT SPECIES BIOLOGY　29　(2)　117-128　2014/05

DOI： 10.1111/1442-1984.12002 　

ISSN： 0913-557X

eISSN： 1442-1984
Floral morphology and pollinator fauna characteristics of island and mainland populations of Ligustrum ovalifolium (Oleaceae) Peer-reviewed

Takayuki Yamada, Kosei Kodama, Masayuki Maki

BOTANICAL JOURNAL OF THE LINNEAN SOCIETY　174　(3)　489-501　2014/03

DOI： 10.1111/boj.12092 　

ISSN： 0024-4074

eISSN： 1095-8339
Phylogeny and historical biogeography of Isodon (Lamiaceae): Rapid radiation in south-west China and Miocene overland dispersal into Africa Peer-reviewed

Xiang-Qin Yu, Masayuki Maki, Bryan T. Drew, Alan J. Paton, Hsi-Wen Li, Jian-Li Zhao, John G. Conran, Jie Li

Molecular Phylogenetics and Evolution　77　(1)　183-194　2014
Publisher: Academic Press Inc.
DOI： 10.1016/j.ympev.2014.04.017 　

ISSN： 1095-9513 1055-7903
Rapid radiation in the Gengduan Mountains region and into Africa with allopolyploidy of Isodon (Lamiaceae). Peer-reviewed

Yu, X.-Q, Maki, M, Drew, B. T, Paton, A. J, Li, H.-W, Zhao, J.-L, Cornan, J. G, Li, J

Molecular Phylogenetics and Evolution　77　183-194　2014

DOI： 10.1016/j.ympev.2014.04.017 　
Population history of the terrestrial orchid Cremastra appendiculata var. variabilis from Korea, inferred from levels and distribution of genetic diversity Peer-reviewed

Mi Yoon Chung, Jordi Lopez-Pujol, Myung-Ok Moon, Masayuki Maki, Tomohisa Yukawa, Naoto Sugiura, Myong Gi Chung

BOTANICAL JOURNAL OF THE LINNEAN SOCIETY　173　(4)　721-732　2013/12

DOI： 10.1111/boj.12109 　

ISSN： 0024-4074

eISSN： 1095-8339
Was Jeju Island a glacial refugium for East Asian warm-temperate plants? insights from the homosporous fern Selliguea hastata (Polypodiaceae) Peer-reviewed

Mi Yoon Chung, Myung-Ok Moon, Jordi López-Pujol, Masayuki Maki, Tadashi Yamashiro, Tomohisa Yukawa, Naoto Sugiura, Yung-I Lee, Myong Gi Chung

American Journal of Botany　100　(11)　2240-2249　2013/11

DOI： 10.3732/ajb.1300134 　

ISSN： 0002-9122
Comparative phylogeographic study of Hosta sieboldiana and Hosta albomarginata (Asparagaceae) in Japan Peer-reviewed

Sangryong Lee, Masayuki Maki

Ecology and Evolution　3　(14)　4767-4785　2013/11

DOI： 10.1002/ece3.838 　

ISSN： 2045-7758
DEVELOPMENT OF MICROSATELLITE MARKERS FOR LEUCOSCEPTRUM JAPONICUM AND L. STELLIPILUM (LAMIACEAE) Peer-reviewed

Yue Li, Masayuki Maki

APPLICATIONS IN PLANT SCIENCES　1　(10)　e1300018　2013/10

DOI： 10.3732/apps.1300038 　

ISSN： 2168-0450
DEVELOPMENT OF MICROSATELLITE MARKERS FOR ISODON LONGITUBUS (LAMIACEAE) Peer-reviewed

Tadashi Yamashiro, Asuka Yamashiro, Ikumi Dohzono, Masayuki Maki

APPLICATIONS IN PLANT SCIENCES　1　(10)　1300028　2013/10

DOI： 10.3732/apps.1300028 　

ISSN： 2168-0450
Genetic variation and structure within 3 endangered calanthe species (Orchidaceae) from Korea: Inference of population-establishment history and implications for conservation Peer-reviewed

Mi Yoon Chung, Jordi López-Pujol, Masayuki Maki, Myung-Ok Moon, Jin Oh Hyun, Myong Gi Chung

Journal of Heredity　104　(2)　248-262　2013/03

DOI： 10.1093/jhered/ess088 　

ISSN： 0022-1503 1465-7333
Pattern of population genetic structure revealed by nuclear simple sequence repeat markers in the understory perennial Veratrum album ssp oxysepalum (Melanthiaceae) with a disjunct pattern of chloroplast DNA haplotypes Peer-reviewed

Ryo Kikuchi, Jae-Hong Pak, Hirosho Takahashi, Masayuki Maki

BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY　108　(2)　278-293　2013/02

DOI： 10.1111/j.1095-8312.2012.02023.x 　

ISSN： 0024-4066
Genetic Diversity in the Common Terrestrial Orchid Oreorchis patens and Its Rare Congener Oreorchis coreana: Inference of Species Evolutionary History and implications for Conservation Peer-reviewed

Mi Yoon Chung, Jordi Lopez-Pujol, Masayuki Maki, Ki-Joong Kim, Jae Min Chung, Byung-Yun Sun, Myong Gi Chung

JOURNAL OF HEREDITY　103　(5)　692-702　2012/09

DOI： 10.1093/jhered/ess032 　

ISSN： 0022-1503
ISOLATION AND CHARACTERIZATION OF MICROSATELLITE MARKERS FROM TIARELLA POLYPHYLLA (SAXIFRAGACEAE) Peer-reviewed

Keum Seon Jeong, Masayuki Maki, Jae-Hong Pak

AMERICAN JOURNAL OF BOTANY　99　(6)　E255-E257　2012/06

DOI： 10.3732/ajb.1100553 　

ISSN： 0002-9122
Impact of geographical isolation on genetic differentiation in insular and mainland populations of Weigela coraeensis (Caprifoliaceae) on Honshu and the Izu Islands Peer-reviewed

Takayuki Yamada, Masayuki Maki

JOURNAL OF BIOGEOGRAPHY　39　(5)　901-917　2012/05

DOI： 10.1111/j.1365-2699.2011.02634.x 　

ISSN： 0305-0270
Quantitative morphological analysis of populations in a hybrid zone of Epimedium diphyllum and E. sempervirens var. rugosum (Berberidaceae) Peer-reviewed

Sachiko Horie, Kazuo Suzuki, Masayuki Maki

PLANT ECOLOGY AND EVOLUTION　145　(1)　88-95　2012

DOI： 10.5091/plecevo.2012.637 　

ISSN： 2032-3913
Seed abortion due to inbreeding depression in the threatened saprophyte Petrosavia sakuraii (Petrosaviaceae) Peer-reviewed

Takahashi H, Maki M

Journal of Phytogeography and Taxonomy　59　(1)　47-50　2011/12
Publisher:
ISSN： 0388-6212
Characterization of polymorphic microsatellite markers isolated from the relict perennial Veratrum stamineum var. micranthum Satake (Melanthiaceae) Peer-reviewed

Ryo Kikuchi, Masayuki Maki

CONSERVATION GENETICS RESOURCES　3　(3)　507-509　2011/07

DOI： 10.1007/s12686-011-9390-y 　

ISSN： 1877-7252
Isolation and characterization of microsatellite loci for Menziesia goyozanensis, an endangered shrub species endemic to Mt. Goyo in northern Japan Peer-reviewed

Harue Abe, Masayuki Maki, Sachiko Horie, Yoshihisa Suyama

CONSERVATION GENETICS RESOURCES　3　(3)　569-571　2011/07

DOI： 10.1007/s12686-011-9406-7 　

ISSN： 1877-7252
Comparison of genetic variation and differentiation using microsatellite markers among three rare threatened and one widespread toad lily species of Tricyrtis section Flavae (Convallariaceae) in Japan Peer-reviewed

Yasuhiro Takahashi, Hiroshi Takahashi, Masayuki Maki

PLANT SPECIES BIOLOGY　26　(1)　13-23　2011/01

DOI： 10.1111/j.1442-1984.2010.00297.x 　

ISSN： 0913-557X
Lack of allozyme diversity in populations of the endangered perennial Senecio scandens (Asteraceae) in Japan: comparison with a population in Taiwan. Peer-reviewed

Maki, M, Kokubugata, G, Yamashiro, T

Journal of Phytogeography and Taxonomy　58　(1)　39-42　2010/12
Publisher:
ISSN： 0388-6212
Molecular phylogeny of Isodon (Lamiaceae) in Japan using chloroplast DNA sequences: recent rapid radiations or ancient introgressive hybridization? Peer-reviewed

Masayuki Maki, Tadashi Yamashiro, Ikumi Dohzono, Kazuo Suzuki

PLANT SPECIES BIOLOGY　25　(3)　240-248　2010/09

DOI： 10.1111/j.1442-1984.2010.00290.x 　

ISSN： 0913-557X
Genetic diversity and population genetic differentiation in the endangered annual weed, Bidens cernua (Compositae), and two common congeners in Japan Peer-reviewed

Ai Tanahara, Masayuki Maki

WEED BIOLOGY AND MANAGEMENT　10　(2)　113-119　2010/06

DOI： 10.1111/j.1445-6664.2010.00374.x 　

ISSN： 1444-6162
Floral differentiation among insular and mainland populations of Weigela coraeensis (Caprifoliaceae) Peer-reviewed

T. Yamada, T. Kashiwagi, M. Sawamura, M. Maki

Plant Systematics and Evolution　288　(1)　113-125　2010

DOI： 10.1007/s00606-010-0317-y 　

ISSN： 0378-2697

eISSN： 1615-6110
Disjunct distribution of chloroplast DNA haplotypes in the understory perennial Veratrum album ssp. oxysepalum (Melanthiaceae) in Japan as a result of ancient introgression Peer-reviewed

Ryo Kikuchi, Pak Jae-Hong, Hiroshi Takahashi, Masayuki Maki

NEW PHYTOLOGIST　188　(3)　879-891　2010

DOI： 10.1111/j.1469-8137.2010.03398.x 　

ISSN： 0028-646X
Intraspecific differentiation of Limonium wrightii (Plumbaginaceae) on northwestern Pacific Islands: Rate heterogeneity in nuclear rDNA and its distance-independent geographic structure Peer-reviewed

Shun'ichi Matsumura, Jun Yokoyama, Tatsuya Fukuda, Masayuki Maki

MOLECULAR PHYLOGENETICS AND EVOLUTION　53　(3)　1032-1036　2009/12

DOI： 10.1016/j.ympev.2009.06.011 　

ISSN： 1055-7903
Origin of the disjunct distribution of flower colour polymorphism within Limonium wrightii (Plumbaginaceae) in the Ryukyu Archipelago Peer-reviewed

Shun'Ichi Matsumura, Jun Yokoyama, Tatsuya Fukuda, Masayuki Maki

Biological Journal of the Linnean Society　97　(4)　709-717　2009/08

DOI： 10.1111/j.1095-8312.2009.01253.x 　

ISSN： 0024-4066 1095-8312
Development of microsatellite markers for the endangered grassland perennial herb Vincetoxicum atratum (Apocynaceae-Asclepiadoideae) Peer-reviewed

Fumito Tada, Tadashi Yamashiro, Masayuki Maki

CONSERVATION GENETICS　10　(4)　1057-1059　2009/08

DOI： 10.1007/s10592-008-9687-x 　

ISSN： 1566-0621
Development of SCAR markers for sex determination in the dioecious shrub Aucuba japonica (Cornaceae) Peer-reviewed

Masayuki Maki

GENOME　52　(3)　231-237　2009/03

DOI： 10.1139/G08-120 　

ISSN： 0831-2796
Development and characterization of 10 microsatellite markers for the semi-evergreen tree species, Ligustrum ovalifolium (Oleaceae) Peer-reviewed

Kosei Kodama, Takayuki Yamada, Masayuki Maki

MOLECULAR ECOLOGY RESOURCES　8　(5)　1008-1010　2008/09

DOI： 10.1111/j.1755-0998.2008.02136.x 　

ISSN： 1755-098X
Allozyme diversity and history of distribution expansion in the maritime perennial plant Hedyotis strigulosa (Rubiaceae), distributed over the wide latitudes in the Japanese Archipelago Peer-reviewed

Masayuki Maki, Shun'ichi Matsumua, Tadashi Yamashiro

BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY　93　(4)　679-688　2008/04

DOI： 10.1111/j.1095-8312.2007.00894.x 　

ISSN： 0024-4066
Morphological aspects and phylogenetic analyses of pollination systems in the Tylophora-Vincetoxicum complex (Apocynaceae-Asclepiadoideae) in Japan Peer-reviewed

Tadashi Yamashiro, Asuka Yamashiro, Jun Yokoyama, Masayuki Maki

BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY　93　(2)　325-341　2008/02

DOI： 10.1111/j.1095-8312.2007.00896.x 　

ISSN： 0024-4066

eISSN： 1095-8312
Isolation and characterization of microsatellite loci from Weigela coraeensis (Caprifoliaceae) Peer-reviewed

Takayuki Yamada, Masayuki Maki

MOLECULAR ECOLOGY RESOURCES　8　(1)　155-157　2008/01

DOI： 10.1111/j.1471-8286.2007.01908.x 　

ISSN： 1471-8278
Isolation and characterization of microsatellite markers from the hosta species Hosta albomarginata (Liliaceae) Peer-reviewed

Hidetoshi Yamamoto, Takayuki Yamada, Masayuki Maki

MOLECULAR ECOLOGY RESOURCES　8　(1)　185-187　2008/01

DOI： 10.1111/j.1471-8286.2007.01918.x 　

ISSN： 1471-8278
Molecular phylogeny and reticulate evolution of Asarum sect. Asiasarum (Aristolochiaceae) documented by chloroplat DNA sequences. Peer-reviewed

Yamaji, H. Fukuda, T. Yokoyama, J. Pak, J.-H. Kondo, K. Morota, T. Maki

Acta Phytotaxomica et Geobotanica　58　(2)　87-96　2007/12
Publisher: The Japanese Society for Plant Systematics
DOI： 10.18942/apg.KJ00004808382 　

ISSN： 1346-7565

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The phylogenetic relationships of Asarum sect. Asiasarum based on genetic variation in non-coding chloroplast DNA sequences were examined. A total of ca. 960 bp sequences of three non-coding regions, namely trnL (UAA) intron, rps16 intron and psbC-trnS (UGA) intergenic region, were analyzed for 37 accessions from 13 taxa and one geographic race. In the maximum-parsimony (MP) tree, four types were recognized. Among the 13 taxa and one geographic race, 10 taxa were identified as belonging to a single type, while the remaining three taxa and one geographic race, A. patens, A. sieboldii f. sieboldii, A. heterotropoides var. seoulense (from Korea) and A. versicolor, belonged to two types. The types all corresponded to more than one ribotype recognized in previous of nrDNA, ITS sequences. The phylogenetic tree topologies were difficult to compare. Chloroplast polymorphisms within a taxon and incongruence of the phylogenetic tree topologies between chloroplast and ITS were considered to be caused by chloroplast capture following hybridization. Reticulate evolution in sect. Asiasarum was therefore inferred to be more extensive than implied from ITS sequence data. Asarum maruyamae was speculated as being of hybrid origin from crosses between A. sieboldii f. sieboldii and A. mikuniense.
Characterization of microsatellite loci for a barrenwort species (Epimedium diphyllum, Berberidaceae) Peer-reviewed

Sachiko Horie, Kazuo Suzuki, Masayuki Maki

Molecular Ecology Notes　7　(6)　1172-1174　2007/11

DOI： 10.1111/j.1471-8286.2007.01820.x 　

ISSN： 1471-8278 1471-8286
Isolation and characterization of microsatellite loci in the threatened wild toad lily Tricyrtis flava (Liliaceae) Peer-reviewed

Yasuhiro Takahashi, Masayuki Maki

MOLECULAR ECOLOGY NOTES　7　(6)　1299-1301　2007/11

DOI： 10.1111/j.1471-8286.2007.01861.x 　

ISSN： 1471-8278
Reticulate evolution and phylogeography in Asarum sect. Asiasarum (Aristolochiaceae) documented in internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA Peer-reviewed

Hiroki Yamaji, Tatsuya Fukuda, Jun Yokoyama, Jae-Hong Pak, Chang-Zheng Zhou, Chun-Shu Yang, Kenji Kondo, Takashi Morota, Shuichi Takeda, Hiroshi Sasaki, Masayuki Maki

MOLECULAR PHYLOGENETICS AND EVOLUTION　44　(2)　863-884　2007/08

DOI： 10.1016/j.ympev.2007.01.011 　

ISSN： 1055-7903
Taxonomic study of Asarum sect. Asiasarum (Aristolochiaceae) in Japan. Peer-reviewed

Yamaji, H. Nakamura, T. Yokoyama, J. Kondo, K. Morota, T. Takeda, S. Sakai, M. Maki

Japanese Journal of Botany　82　57-78　2007/02
Morphological analysis of Asarum sect. Asiasarum (Aristolochiaceae) in Japan, with special reference to multivariate analyses of flowers. Peer-reviewed

Yamaji, H. Nakamura, T. Yokoyama, J. Kondo, K. Morota, T. Takeda, S. Sakai, M. Maki

Japanese Journal of Botany　82　79-105　2007/02
Morphological and molecular (RAPD) analyses confirm the hybrid origin of the diploid grass Calamagrostis longiseta var. longe-aristata (Gramineae) Peer-reviewed

Kaku Saitou, Tatsuya Fukuda, Jun Yokoyama, Masayuki Maki

FOLIA GEOBOTANICA　42　(1)　63-76　2007

DOI： 10.1007/BF02835102 　

ISSN： 1211-9520
Intraspecific variation of flower colour and its distribution within a sea lavender, Limonium wrightii (Plumbaginaceae), in the northwestern Pacific Islands Peer-reviewed

Shun-ichi Matsumura, Jun Yokoyama, Yoichi Tateishi, Masayuki Maki

JOURNAL OF PLANT RESEARCH　119　(6)　625-632　2006/11

DOI： 10.1007/s10265-006-0022-7 　

ISSN： 0918-9440
The structure and expression of SEPALLATA-like genes in Asparagus species (Asparagaceae) Peer-reviewed

Akira Kanno, Homare Hienuki, Takuro Ito, Toru Nakamura, Tatsuya Fukuda, Pil-Yong Yun, In-Ja Song, Taichi Kamimura, Toshinori Ochiai, Jun Yokoyama, Masayuki Maki, Toshiaki Kameya

SEXUAL PLANT REPRODUCTION　19　(3)　133-144　2006/09

DOI： 10.1007/s00497-006-0030-8 　

ISSN： 0934-0882

eISSN： 1432-2145
Morphological and habitat divergence in the intertidal limpet Patelloida pygmaea Peer-reviewed

Shizuko Nakai, Osamu Miura, Masayuki Maki, Satoshi Chiba

MARINE BIOLOGY　149　(3)　515-523　2006/06

DOI： 10.1007/s00227-005-0241-9 　

ISSN： 0025-3162
Concordant clines and significant correlation between floral and pollen characters in Asarum heterotropoides var. heterotropoides (Aristolochiaceae) Peer-reviewed

H Yamaji, J Yokoyama, H Ohashi, M Maki

PLANT SYSTEMATICS AND EVOLUTION　259　(1)　1-17　2006/05

DOI： 10.1007/s00606-005-0405-6 　

ISSN： 0378-2697
A comparative study of the reproductive character and genetic diversity of an autogamous Tylophora matsumurae and its progenitor Tylophora tanakae (Apocynaceae-Asclepiadoideae) Peer-reviewed

T Yamashiro, M Maki

PLANT SYSTEMATICS AND EVOLUTION　256　(1-4)　55-67　2005/11

DOI： 10.1007/s00606-005-0360-2 　

ISSN： 0378-2697
Chromosome numbers of Isodon (Lamiaceae) in Japan Peer-reviewed

Yamashiro, T. Suzuki, K. Maki

Acta Phytotaxomica et Geobotanica　56　(3)　241-246　2005/09
Publisher: The Japanese Society for Plant Systematics
DOI： 10.18942/apg.KJ00004623255 　

ISSN： 1346-7565

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Somatic chromosome numbers were counted in seven species and six varieties of Isodon occurring in Japan. All of the taxa had 2n=24 chromosomes, indicating that they are diploid. For all taxa except I. umbrosus var. hakusanensis and I. japonicus, chromosome numbers are reported here for the first time. The results suggest that diversification in Japanese taxa of Isodon has occurred at the diploid level, even though some reticulation had been suggested by a preliminary molecular study.
Characterization of TrcMADS1 gene of Trillium camtschatcense (Trilliaceae) reveals functional evolution of the SOC1/TH3-like gene family Peer-reviewed

T Nakamura, IJ Song, T Fukuda, J Yokoyama, M Maki, T Ochiai, T Kameya, A Kanno

JOURNAL OF PLANT RESEARCH　118　(3)　229-234　2005/06

DOI： 10.1007/s10265-005-0215-5 　

ISSN： 0918-9440
Molecular phylogeny and evolution of alcohol dehydrogenase (Adh) genes in legumes Peer-reviewed

Tatsuya Fukuda, Jun Yokoyama, Toru Nakamura, In-Ja Song, Takuro Ito, Toshinori Ochiai, Akira Kanno, Toshiaki Kameya, Masayuki Maki

BMC PLANT BIOLOGY　5　Art. No6　2005/04

DOI： 10.1186/1471-2229-5-6 　

ISSN： 1471-2229
No genetic differentiation between Asarum muculatum and A. sieboldii in Korea (Aristolochiaceae). Peer-reviewed

Yamaji, H, Yokoyama, J, Pak, J.-H, Maki, M

Acta Phytotaxnomica et Geobotanica　55　(3)　199-205　2004/12
Publisher: The Japanese Society for Plant Systematics
DOI： 10.18942/apg.KJ00004622829 　

ISSN： 1346-7565

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Asarum maculatum Nakai, a perennial herb endemic to southern Korea, is unique in its variegated leaves. However, the independence of this species is doubtful because the species co-occurs with A. sieboldii Miq. var. sieboldii in the same geographical area in Korea, and no significant difference is found between the two taxa in external morphology, except for leaf variegation. We examined the genetic differentiation between A. maculatum and A. sieboldii van sieboldii using enzyme electrophoresis. The genetic differentiation between the two taxa in the same locality was not greater than that among the intraspecific populations. Moreover, there is little genetic differentiation between the mixed populations of the two taxa and the populations composed only of A. sieboldii var. sieboldii. Consequently, A. maculatum is not differentiated genetically from A. sieboldii var. sieboldii, and the two taxa should be regarded as the same species. Hence, a new combination, A. sieboldii f. maculatum (Nakai) Yamaji (comb, et stat. nov.), is proposed.
Expansion of distribution range and its pollinators of Canavalia rosea (Leguminosae) in the Ryukyu Archipelago, subtropical Eastern Asia. Peer-reviewed

Matsumura, S.-I, Tateishi, Y, Yokoyama, J, Maki, M

Acta Phytotaxonomica et Geobotanica　55　(3)　207-212　2004/12
Publisher: The Japanese Society for Plant Systematics
DOI： 10.18942/apg.KJ00004622830 　

ISSN： 1346-7565

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New localities of Canavalia rosea were found on Okinawa and in the Amami Islands of the Ryukyu Archipelago. The locality on Amami-Oshima marks the northern-most limit of the species in Asia and extends its range approximately 490 km northeastward. At its newly discovered locations, C. rosea is pollinated by female leaf cutter bees and bears fruit. We suspect that the presence of three closely related species of Canavalia with flowers morphologically similar to those of C. rosea is probably one of the main reasons for the visitation by leaf cutter bees to C. rosea in the Ryukyu Archipelago. The female bees exhibited two patterns of behavior during flower visitations and effectively pollinated the flowers of C. rosea only when collecting both pollen and nectar. Pollinators of C. rosea have been reported as being anthopholid bees elsewhere, but are leaf cutter bees in the Ryukyu Archipelago.
Evolution of floral dimorphism in a cleistogamous annual, Impatiens noli-tangere L. occurring under different environmental conditions Peer-reviewed

M Masuda, T Yahara, M Maki

ECOLOGICAL RESEARCH　19　(6)　571-580　2004/11

DOI： 10.1111/j.1440-1703.2004.00673.x 　

ISSN： 0912-3814
An under-appreciated difficulty: sampling of plant populations for analysis using molecular markers Peer-reviewed

JI Suzuki, T Herben, M Maki

EVOLUTIONARY ECOLOGY　18　(5-6)　625-646　2004/09

DOI： 10.1007/s10682-004-5147-3 　

ISSN： 0269-7653
Two new species of Vincetoxicum(Apocynaceae-Asclepioideae) from Japan. Peer-reviewed

Yamashiro, T, Yokoyama, J, Maki, M

Acta Phytotaxonomica et Geobotanica　55　(1)　1-8　2004/06
Publisher: The Japanese Society for Plant Systematics
DOI： 10.18942/apg.KJ00004622799 　

ISSN： 1346-7565

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Two new species, Vincetoxicum izuense and V. hoyoense, are described here. Vincetoxicum izuense is a twining perennial herb on rocky beaches and on the margins of thickets near the sea and is endemic to the southern Izu Peninsula. It resembles V. sublanceolatum and V. japonicum, but V. izuense is distinguished from them by the leaves, follicles and trichomes on the lower surface of the leaves. Vincetoxicum hoyoense is a large, perennial, twining herb on rocks and on the margins of thickets facing the sea and is endemic to the area around the Bungo Channel. Vincetoxicum hoyoense resembles V. japonicum f. puncticulatum and V. sublanceolatum, but V. hoyoense can be distinguished from them by the larger, elliptic or ovate leaf blades with shallowly cordate base and the longer corolla lobes. Although V. hoyoense also resembles V. izuense in flower, morphology and leaf shape, V. hoyoense is easily distinguished from it by the large ovate or elliptic leaves with shallowly cordate base and the large corollas.
Molecular phylogeny of Vincetoxicum (Apocynaceae-Asclepladoideae) based on the nucleotide sequences of cpDNA and nrDNA Peer-reviewed

T Yamashiro, T Fukuda, J Yokoyama, M Maki

MOLECULAR PHYLOGENETICS AND EVOLUTION　31　(2)　689-700　2004/05

DOI： 10.1016/j.ympev.2003.08.016 　

ISSN： 1055-7903
Transferance of Vincetoxicum matsumurae (Yamazaki) Ohashi to Tylophora. Peer-reviewed

Yamashiro, T, Tateishi, Y, Denda, T, Yokota, M, Maki, M

Japanese Journal of Botany　78　(6)　349-354　2003/12
Publisher:
ISSN： 0022-2062
Molecular evolution of cycloidea-like genes in Fabaceae Peer-reviewed

T Fukuda, J Yokoyama, M Maki

JOURNAL OF MOLECULAR EVOLUTION　57　(5)　588-597　2003/11

DOI： 10.1007/s00239-003-2498-2 　

ISSN： 0022-2844
High levels of genetic diversity in island populations of the island endemic Suzukia luchuensis (Labiatae) Peer-reviewed

M Maki, T Yamashiro, S Matsumura

HEREDITY　91　(3)　300-306　2003/09

DOI： 10.1038/sj.hdy.6800311 　

ISSN： 0018-067X
Vincetoxicum yonakuniense, a new combination of Asclepiadaceae and a new record on Senkaku Islands, Ryukyu Archipelago. Acta Phytotaxonomica et Geobotanica Peer-reviewed

Yamashiro, T, Tateishi, Y, Maki, M

Acta Phytotaxonomica et Geobotanica　54　(1)　31-36　2003/06
Publisher: The Japanese Society for Plant Systematics
DOI： 10.18942/apg.KJ00004623203 　

ISSN： 1346-7565

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Cynanchum yonakuniense, a perennial twining herb, endemic to Yonaguni Island, the Ryukyu Archipelago has never been recorded since its original materials collected there. We rediscovered it on the island and moreover found a herbarium specimen of the species on Senkaku Islands in the same archipelago. In this paper we added the morphologies of root, fruit and seed to the description of this species. On the basis of morphological re-examinations, we propose a new combination Vincetoxicum yonakuniense.
Population genetics of threatened wild plants in Japan Invited Peer-reviewed

M Maki

JOURNAL OF PLANT RESEARCH　116　(2)　169-174　2003/04

DOI： 10.1007/s10265-003-0083-9 　

ISSN： 0918-9440
Chromosome numbers of Japanese Asclepiadaceae Peer-reviewed

Yamashiro, T, Yokota, M, Denda, T, Tateishi, Y, Yokoyama, M, Maki, M

Acta Phytotaxonomica et Geobotanica　53　(2)　143-152　2002/06
Publisher: The Japanese Society for Plant Systematics
DOI： 10.18942/apg.KJ00003256682 　

ISSN： 1346-7565

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Somatic chromosome numbers were examined for 28 species and two varieties of seven genera in Asclepiadaceae collected in Japan. The chromosome numbers of 21 species and two varieties were newly reported here. All taxa examined had chromosome numbers of 2n=22 except for 2n-44 in Cynanchum caudatum var. caudatum, Vincetoxicum ambiguum, V. sublanceolatum var. sublanceolatum, V. sublanceolatum var. macranthum and 2n=24 for Cynanchum boudieri. In Cynanchum caudatum, chromosome number of 2n=22 was also found as well as 2n=44, indicating that intraspecific polyploidy exists in the species.
The intersectional hybrid between Weigela hortensis and W. maximowiczii (Caprifoliaceae) Peer-reviewed

Jun Yokoyama, Tatsuya Fukuda, Akiko Yokoyama, Masayuki Maki

Botanical Journal of the Linnean Society　138　(3)　369-380　2002/03

DOI： 10.1046/j.1095-8339.2002.00033.x 　

ISSN： 0024-4074
Comparison of genetic diversity between narrowly endemic shrub Menziesia goyozanensis and its widespread congener M-pentandra (Ericaceae) Peer-reviewed

M Maki, S Horie, J Yokoyama

CONSERVATION GENETICS　3　(4)　421-425　2002

DOI： 10.1023/A:1020546516111 　

ISSN： 1566-0621
An ESS model for the mixed production of cleistogamous and chasmogamous flowers in a facultative cleistogamous plant. Peer-reviewed

Masuda, M, Yahara, T, Maki, M

Evolutionary Ecology Research　3　429-439　2001/06
Allozyme analysis of the hybrid origin of Arisaema ehimense (Araceae) Peer-reviewed

Masayuki Maki, Jin Murata

Heredity　86　(1)　87-93　2001

DOI： 10.1046/j.1365-2540.2001.00813.x 　

ISSN： 0018-067X
Genetic differentiation within and among island populations of the endangered plant Aster miyagii (Asteraceae), an endemic to the Ryukyu Islands Peer-reviewed

Masayuki Maki

American Journal of Botany　88　(12)　2189-2194　2001
Publisher: Botanical Society of America Inc.
DOI： 10.2307/3558380 　

ISSN： 0002-9122
Random amplified polymorphic DNA (RAPD) variation among populations of the insular endemic plant Campanula microdonta (Campanulaceae) Peer-reviewed

S. Oiki, T. Kawahara, K. Inoue, M. Ohara, M. Maki

Annals of Botany　87　(5)　661-667　2001
Publisher: Academic Press
DOI： 10.1006/anbo.2001.1389 　

ISSN： 0305-7364
Effects of salinity and temperature on seed germination in a Japanese endangered halophyte Triglochin maritimum (Juncaginaceae) Peer-reviewed

M Masuda, M Maki, T Yahara

JOURNAL OF PLANT RESEARCH　112　(1108)　457-461　1999/12

DOI： 10.1007/PL00013901 　

ISSN： 0918-9440
The effect of geographic range and dichogamy on genetic variability and population genetic structure in Tricyrtis section Flavae (Liliaceae) Peer-reviewed

M Maki, H Morita, S Oiki, H Takahashi

AMERICAN JOURNAL OF BOTANY　86　(2)　287-292　1999/02

DOI： 10.2307/2656945 　

ISSN： 0002-9122
Genetic diversity in the threatened insular endemic plant Aster asa-grayi (Asteraceae) Peer-reviewed

M Maki

PLANT SYSTEMATICS AND EVOLUTION　217　(1-2)　1-9　1999

ISSN： 0378-2697
Random amplified polymorphic DNA (RAPD) markers reveal less genetic variation in the endangered plant Cerastium fischerianum var. molle than in the widespread conspecific C-fischerianum var. fischerianum (Caryophyllaceae) Peer-reviewed

M Maki, S Horie

MOLECULAR ECOLOGY　8　(1)　145-150　1999/01

DOI： 10.1046/j.1365-294X.1999.00517.x 　

ISSN： 0962-1083
Inbreeding depression and outcrossing rate in the endangered autotetraploid plant Aster kantoensis (Asteraceae) Peer-reviewed

K Inoue, M Masuda, M Maki

JOURNAL OF HEREDITY　89　(6)　559-562　1998/11

ISSN： 0022-1503
Identification of conservation measures to protect the Japanese endangered plant species Aster kantoensis Peer-reviewed

K Inoue, N Kuramoto, M Maki, M Masuda, Washitani, I

ECOLOGICAL RESEARCH　13　(2)　141-149　1998/08

DOI： 10.1046/j.1440-1703.1998.00253.x 　

ISSN： 0912-3814
High genetic variability revealed by allozymic loci in the narrow endemic fern Polystichum otomasui (Dryopteridaceae) Peer-reviewed

M Maki, YJ Asada

HEREDITY　80　604-610　1998/05

ISSN： 0018-067X
Genetic variation in relic and isolated populations of Chionanthus retusus (Oleaceae) of Tsushima Island and the Tono region, Japan Peer-reviewed

A Soejima, M Maki, K Ueda

GENES & GENETIC SYSTEMS　73　(1)　29-37　1998/02

DOI： 10.1266/ggs.73.29 　

ISSN： 1341-7568

eISSN： 1880-5779
Genetic diversity in island and mainland populations of Aster spathulifolius (Asteraceae) Peer-reviewed

M. Maki, H. Morita

International Journal of Plant Sciences　159　(1)　148-152　1998

DOI： 10.1086/297532 　

ISSN： 1058-5893
Spatial structure of genetic variation in a population of the endangered plant Cerastium fischerianum var. molle (caryophyllaceae) Peer-reviewed

M Maki, T Yahara

GENES & GENETIC SYSTEMS　72　(4)　239-242　1997/08

DOI： 10.1266/ggs.72.239 　

ISSN： 1341-7568

eISSN： 1880-5779
Chromosome numbers of two Japanese Impatiens. Peer-reviewed

Tagawa, K, Araki, H, Maki, M

Journal of Phytogeography and Taxonomy　45　32-34　1997/06
Genetic diversity and hierarchical population structure of a rare autotetraploid plant, Aster kantoensis (Asteraceae) Peer-reviewed

M Maki, M Masuda, K Inoue

AMERICAN JOURNAL OF BOTANY　83　(3)　296-303　1996/03

DOI： 10.2307/2446164 　

ISSN： 0002-9122
Differences in plant size and flower production between hermaphrodites and females of two gynodioecious Chionographis (Liliaceae) Peer-reviewed

Masayuki Maki

Canadian Journal of Botany　74　(1)　150-153　1996
Publisher: National Research Council of Canada
DOI： 10.1139/b96-020 　

ISSN： 0008-4026
Tetrasomic segregation of allozyme markers in an endangered plant, Aster kantoensis Peer-reviewed

M. Maki, M. Masuda, K. Inoue

Journal of Heredity　87　(5)　378-380　1996
Publisher: Oxford University Press
DOI： 10.1093/oxfordjournals.jhered.a023017 　

ISSN： 0022-1503
Different responses of pollinating bees to size variation and sexual phases in flowers of Campanula Peer-reviewed

Ken Inoue, Masayuki Maki, Michiko Masuda

Ecological Research　10　(3)　267-273　1995/12
Publisher: Springer-Verlag
DOI： 10.1007/BF02347852 　

ISSN： 0912-3814 1440-1703
SPATIAL GENETIC-STRUCTURE WITHIN 2 POPULATIONS OF A SELF-INCOMPATIBLE PERENNIAL, CHIONOGRAPHIS-JAPONICA VAR JAPONICA (LILIACEAE) Peer-reviewed

M MAKI, M MASUDA

JOURNAL OF PLANT RESEARCH　107　(1087)　283-287　1994/09

DOI： 10.1007/BF02344256 　

ISSN： 0918-9440
EFFECTS OF BIPARENTAL INBREEDING ON THE EVOLUTION OF GYNODIOECY - A MODEL AND A CASE-STUDY IN CHIONOGRAPHIS-JAPONICA VAR KUROHIMENSIS Peer-reviewed

T YAHARA, M MAKI

JOURNAL OF PLANT RESEARCH　106　(1083)　279-281　1993/09

ISSN： 0918-9440
FLORAL SEX-RATIO VARIATION IN HERMAPHRODITES OF GYNODIOECIOUS CHIONOGRAPHIS-JAPONICA VAR KUROHIMENSIS AJIMA-ET-SATOMI (LILIACEAE) Peer-reviewed

M MAKI

JOURNAL OF PLANT RESEARCH　106　(1082)　181-186　1993/06

ISSN： 0918-9440
Outcrossing and fecundity advantage of females in gynodioecious Chionographis japonica var. kurohimensis (Liliaceae). Peer-reviewed

Maki M

American Journal of Botany　80　629-634　1993/05

DOI： 10.2307/2445432 　
Pollen‐mediated Gene Flow in a Population of Cynanchum grandifolium var. nikoense (Asclepiadaceae) Peer-reviewed

MASAYUKI MAKI, MICHIKO MASUDA

Plant Species Biology　8　(1)　45-50　1993

DOI： 10.1111/j.1442-1984.1993.tb00233.x 　

ISSN： 1442-1984 0913-557X
Spatial autocorrelation of genotypes in a gynodioecious population of Chionographis japonica var. kurohimensis (Liliaceae) Peer-reviewed

M. Maki, M. Masuda

International Journal of Plant Sciences　154　(4)　467-472　1993

DOI： 10.1086/297130 　

ISSN： 1058-5893
Allozyme variation in Japanese species of Chionographis(Liliaceae). Peer-reviewed

Maki, M

Botanical Magazine Tokyo　105　149-160　1992/09
FIXATION INDEXES AND GENETIC DIVERSITY IN HERMAPHRODITIC AND GYNODIOECIOUS POPULATIONS OF JAPANESE CHIONOGRAPHIS (LILIACEAE) Peer-reviewed

M MAKI

HEREDITY　68　329-336　1992/04

ISSN： 0018-067X
Sex Allocation Ratio in a Wind‐Pollinated Self‐Incompatible Monoecious Tree, Alnus firma Sieb. et Zucc. (Betulaceae) Peer-reviewed

NORIAKI MURAKAMI, MASAYUKI MAKI

Plant Species Biology　7　(2-3)　97-101　1992

DOI： 10.1111/j.1442-1984.1992.tb00223.x 　

ISSN： 1442-1984 0913-557X

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Misc. 29

A record of Chrysosplenium nagasei var. nagasei from Oki Islands Peer-reviewed

Shinji FUJII, Kanna TACHIBANA, Masayuki MAKI

J. Phytogeogr. Taxon.　71　(2)　175-178　2023/11

DOI： 10.18942/chiribunrui.0712-13 　
長野県産マダイオウの標本(国立科学博物館蔵)を見いだす．長野県植物研究会誌

藤井伸二, 牧雅之

長野県植物研究会誌　51　1-2　2018/05
コウガイセキショウモを沖縄本島に記録する

藤井伸二, 赤井賢成, 牧雅之

水草研究会誌　107　41-42　2018
熊本県で観察されたオオカナダモの殖芽様シュートの形成

藤井伸二, 牧雅之

水草研究会誌　107　39-40　2018
New records of naturalized population of Vallisneria × pseudorosulata from Kanagawa and Okayama Prefecture Peer-reviewed

17　(1)　43-47　2017
Publisher: 日本植物分類学会
DOI： 10.18942/bunrui.01701-05 　

ISSN： 1346-6852
マダイオウと雑種ノダイオウの混乱

藤井伸二, 牧雅之

長野県植物研究会誌　50　31-35　2017
宮城県の野生植物を用いた遺伝学的解析

牧雅之

宮城の植物　41　1-4　2016
Newly naturalized plant Vallisneria × pseudorosulata, and identification of V. australis

103　(103)　8-12　2016
Publisher: 水草研究会
ISSN： 1348-4761
愛媛県伊予三島産のハマネナシカズラ標本を見いだす．

藤井伸二, 小林真吾, 牧雅之

エヒメアヤメ（愛媛植物研究会誌）　46　13-14　2015
New records of three plant species (Eleocharis dulcis, Rumex longifolius and Persicaria erectominor) from Shimane Pref. with some notes on P. erectominor var. erectominor f. viridiflora Peer-reviewed

FUJII Shinji, MAKI Masayuki, KUNII Hidenobu

BUNRUI　14　(2)　169-176-176　2014
Publisher: Japanese Society for Plant Systematics
DOI： 10.18942/bunrui.KJ00009573000 　

ISSN： 1346-6852

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Eleocharis dulcis, Rumex longifolius and Persicaria erectominor were newly found from Shimane Pref. The Hii River in Izumo City revealed the western limit of the distribution of R. longifolius and the Lake Nakaumi is the second record of P. erectominor from the area along the Sea of Japan in Kinki-Chugoku District. Persicaria erectominor var. erectominor f. viridiflora showed some difference from f. erectominor on phenology, morphology and distribution.
Brief introduction of molecular phylogenetics in plants Invited Peer-reviewed

49　(49)　17-20　2014
Publisher: 日本植物園協会
ISSN： 0389-5246
Genetic differentiation among the populations of Epimedium diphyllum (C. Morren et Decne) Lodd. var. kitamuranum (T. Yamanaka) K. Suzuki in Awaji-shima and Shikoku

Plants of Hyogo　20　(20)　27-30　2010
Publisher: 兵庫県植物誌研究会
Natural hybridizations in the plant species occurring in Tohoku District

MAKI Masayuki

BUNRUI　10　(1)　23-29　2010
Publisher: Japanese Society for Plant Systematics
DOI： 10.18942/bunrui.KJ00006159261 　

ISSN： 1346-6852
日本の絶滅危惧樹木シリーズ（3）ヒトツバタゴ

牧雅之

林木の育種　204　33-34　2002/04
日本産稀少野生植物の集団遺伝学的研究

牧雅之

生物科学ニュース　370　植55-植56　2002/04
多摩川のカワラノギクのための緊急アピール Peer-reviewed

倉本宣, 石濱史子, 鷲谷いづみ, 嶋田正和, 可知直毅, 井上健, 加賀屋美津子, 牧雅之, 竹中明夫, 増田理子

保全生態学研究　5　(2)　191-196　2000/06
被子植物における性表現の可塑性

牧雅之

蛋白質核酸酵素　43　1112-1118　1998/10
植物の性表現-利己的遺伝子としての雄性不稔

牧雅之

遺伝　52　34-38　1998/01
Recent Progresses in Studies on Recombinational Speciation

MAKI Masayuki

13　(1)　17-23　1997/11/20

ISSN： 0911-6052
遺伝子レベルから見た生物多様性とその保全

牧雅之

遺伝　別冊9　(9)　23-30　1997/06
Publisher: エヌ・ティー・エス
ISSN： 1340-7376
組み換え種分化に関する研究の現状

牧雅之

日本植物分類学会報　13　34-38　1997/06
花の性-なぜ性があるのか

牧雅之

週刊朝日百科「植物の世界」　12　293　1996/12
シライトソウ属の繁殖様式

牧雅之

週刊朝日百科「植物の世界」　10　139　1996/10
Genetic diversity and its conservation in populations of rare wild plants

MAKI M.

37　31-34　1995/11
生物集団の遺伝的多様性の減少

牧雅之, 増田理子

科学　64　(10)　641-648　1994/10
Publisher: 岩波書店
ISSN： 0022-7625
The Seed Population Dynamics of Aster kantoensis in the Floodplain of the Tama River(PAPERS OF THE 12th SCIENTIFIC RESEARCH MEETING) Peer-reviewed

KURAMOTO Noboru, WASHITANI Izumi, MAKI Masayuki, MASUDA Michiko, INOUE Ken

Journal of The Japanese Institute of Landscape Architecture　57　(5)　127-132　1994
Publisher: The Japanese Institute of Landscape Architecture
DOI： 10.5632/jila1934.57.5_127 　

ISSN： 0387-7248

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Seed population dynamics of A. kantoensis were investigated in the floodplain of Tama River during 1991 and 1993. Mean number of seeds (achenes) per head and mean percentage of fertile seeds was 67.6 and 55.3%, respectively. Seedrains (number of fertile seeds per unit area) at the eight populations were estimated to be as 1,400-12,000/m^2. Seed predation by Coleophora were observed to reduce mature seeds. Hence, the effective seed-rains (fertile seeds-preda-ted seeds) were estimated to be as 1,200-11,OOO/m^2. Most (95-99%) of seeds were germinated in spring, remaining a small seed-bank of this species.
植物の非対称な性表現

牧雅之, 矢原徹一

化学と生物　31　242-245　1993/10

DOI： 10.1271/kagakutoseibutsu1962.31.242 　
植物の性発現

牧雅之

遺伝　47　54-58　1993/02
<Bull. Natn. Sci. Mus., Tokyo>A Study in Distribution, Habitat, Character Variation of Dryopteris erythrosora (Aspidiaceae) of the Izu Peninsula

NAKAIKE Toshiyuki, YAMAMOTO Akira, MAKI MASAYUKI

Bulletin of the National Science Museum. Series B, Botany　10　(4)　191-202　1984/12
Publisher: National Science Museum
ISSN： 0385-2431

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The lzu Peninsula is the type locality of Dryopteris erythrosora. In this region, 86 specimens of this species were collected from 68 areas for description; discussion of distribution, habitat, condition, character variation; and examination of reproductive type. Distribution map is shown. The habitat areas lie between 5 and 680 m above sea level, on the floor of an artificial forest of Cryptomeria japonica, Chamaecyparis abtusa and bamboo, along roadsides, on the edge of a Quercus serrata grove, and on the floor of a Castanopsis cuspidata var. sieboldii grove, etc. The number of stocks of this species were observed in each area. In the 10 areas, only 1 stock of this species was discoved. All specimens belong to the apogamous type. The following characters are observed and described in each specimen : color scale at the basal part of stipe, color of lower side of stipe, shape of pinnule, color of indusium, length of basal pinnule and second pinnule of anterior side of basal pinna, and position of sorus. As a result, 24 characters are observed. The 86 specimens are divided into 57 groups based on the combinations of characters mentioned above. No relationships are observed between the external morphology, distribution, and habitat condition.

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Books and Other Publications 7

Flora of Japan volume IVb

Iwatsuki, K, Yamazaki, T, Boufford, D, Ohba

Kodansha　2016
DNAから生き物の過去がよみがえる

種生物学会

2013
新しい植物分類学I

戸部博, 田村実

2012/03
保全と復元の生物学

種生物学会

文一総合出版　2002/10
森の木の100不思議

日本林業技術協会

日本林業技術協会　1996/02
Floral Biology

Lloyd, D. G, Barrett, S. C. H

Chapman and Hall　1995/01
昆虫を誘い寄せる戦略

井上健, 湯本貴和

1992/11

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Research Projects 35

沿岸海洋島における固有植物の初期的種分化の解明：いつ，どこで，なぜ，どのように？

堀江佐知子, 藤井伸二, 牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2022/04/01 - 2025/03/31
近交弱勢と繁殖形質の集団内変異：その維持要因と進化的意義

酒井聡樹, 牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2022/04/01 - 2025/03/31
大規模撹乱と野生植物集団の遺伝的多様性：巨大津波を想定した前向き・後ろ向き研究

牧雅之, 伊東拓朗, 藤井伸二, 山城考

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2022/04/01 - 2025/03/31
宍道湖流域の水草相の過去から現在～環境DNAを用いた広域解析～

小室隆, 藤井伸二, 牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 国立研究開発法人海上・港湾・航空技術研究所

2021/04/01 - 2024/03/31
多様な繁殖段階で生じる形質置換の進化過程の解明

堂囿いくみ, 牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東京学芸大学

2020/04/01 - 2024/03/31

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種間の繁殖干渉によって生じる生殖的形質置換は，種分化や多種共存の重要な要因である。形質置換は交配前の段階に生じることが多いが，交配後・接合前の段階に生じる場合も報告されつつある。しかし，形質置換が生じる段階がなぜさまざまなのか，どのような要因によってもたらされるのかについては，研究例が少ない。本研究では，イヌヤマハッカ（シソ科ヤマハッカ属）と近縁２種それぞれとの共存域における繁殖干渉と生殖的形質置換を明らかにし，多様な形質置換の背景にある生態学的，進化学的プロセスを検証することを目的として研究をおこなった。昨年度同様，長野県北部にて，イヌヤマハッカとクロバナヒキオコシが同所的に分布する野外調査をおこなった。訪花頻度の観察と交配実験（異種交配と混合花粉交配交配による種子生産）を行った。また，異種交配と混合花粉交配による花粉管の伸長を解析している。
地球温暖化の生物間相互作用への影響－環境DNA解析で植物利用者の変化を解明する

牧雅之, 藤井伸二, 森長真一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的研究(萌芽)

Institution: 東北大学

2020/07/30 - 2023/03/31

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近年憂慮されている地球温暖化は，野生生物の生存についても大きな影響を与えると考えられている．植物は動物と比較して移動能力に乏しいため，気温の上昇に即応して分布を移動させることが困難であるが，植物と共生関係にある多くの動物は，気温の上昇に対して，分布を迅速に北上させることが可能である．このミスマッチが生物間相互作用を破綻させ，植物集団の存続を危うくする可能性がある．本研究では，環境DNAの手法を植物体そのものに応用して，分布の南端における生物間相互作用が，分布中心域とどのように違うのかを定量評価して，地球温暖化が植物集団へ及ぼす影響を予測することを目指す．そのために，環境DNAによる生物間相互作用の解析手法の確立を目指し，植物とそれを利用する既知の動物の遺伝的バーコーディングを行い，種判別法を確立する．すでに採取済みのサンプルを用いて，これまでによく用いられてきた分子種を用いて，既知の動物が種判別できることを確認しておく．また，メタゲノムバーコーディングを行うためのDNA抽出ならびにPCR増幅の条件設定について検討する必要がある．さらに，植物と共生する動物が気候変動に伴い，植物の分布変遷とどのように協調して，分布を変遷させてきたかを明らかにすることも重要な課題である．平成21年度は，前年度から引き続き，イヌビワの嚢果からDNAを抽出し，共生すると考えられる動物のミトコンドリアDNAを増幅するプライマーを探索し，実験条件を検討した．結果として，イヌビワコバチ以外にいくつかの節足動物種のDNAが検出された．また，イヌビワとイヌビワを利用するイヌビワシギゾウムシを国内全域からサンプリングし，両者の集団系統樹を作成した．その結果，イヌビワとイヌビワシギゾウムシは，過去の気候変動下において，同調的に分布変遷を行っている可能性が示唆された．
バック・トゥ・ザ・ホンシュウ：伊豆諸島起源植物の本州への逆移入と定着過程を探る

堀江佐知子, 藤井伸二, 牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2019/04/01 - 2023/03/31

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伊豆諸島は本州と陸続きになったことがない海洋島で，いくつかの固有植物種が知られている．それらは本州に分布する祖先種が伊豆諸島に移入した後，島嶼域での隔離によって生じたものと考えられる．しかし，本州と伊豆諸島の距離を考えると伊豆諸島で分化した固有種が再び本州に逆移入して定着する可能性があり得るが，実証的な研究例はない．本研究で研究対象とするユキタノシタ科の多年生草本であるイズノシマダイモンジソウ（Saxifraga fortuneii var. jotanii(Honda) Wakab.）は，伊豆諸島全島と房総半島のみに分布する植物である．この分布パターンは，この種が祖先種であるダイモンジソウ（国内に広く分布）から伊豆諸島で分化した後，本州（房総半島）へ逆移入・定着してきたことを想起させる．本研究では，(1)イズノシマダイモンジソウでは伊豆諸島から本州への逆移入が起きたのか，(2)房総半島ではなぜダイモンジソウは分布せず，イズノシマダイモンジソウだけが分布するのか，(3)イズノシマダイモンジソウが祖先種のダイモンジソウから分化してからどれくらいの時間が経過しているのかを，分子データと人工交配実験を用いて明らかにすることを目指している．これまでの遺伝学的解析により，イズノシマダイモンジソウとその母種であるダイモンジソウは，遺伝的に明確に区別することができ，狭義ダイモンジソウ集団のうち伊豆半島の集団は，伊豆諸島に近いにもかかわらずイズノシマダイモンジソウ集団との分化が大きく，伊豆半島と伊豆諸島の集団間では遺伝的交流は限定的であると推測された．今後，さらに詳細な遺伝学的解析および人工交配実験を行うことでイズノシマダイモンジソウが伊豆諸島で分化した後に本州に逆移入・定着したことを検証する．
Assessment of the impact of spreading agriculture on aquatic plants by phytogeographical methods

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: University of Human Environments

2019/04/01 - 2023/03/31
自殖と近交弱勢の関係の新理論を基軸とした、植物の繁殖戦略の多様性の統一理解

酒井聡樹, 牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2019/04/01 - 2022/03/31

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進化生物学における一つの常識的な知見として、「自殖集団では有害遺伝子が排除される」というものがある。自殖をすると、劣勢有害遺伝子がホモになって生存力が低下する種子が出現しやすい。そうした種子が死亡することで有害遺伝子が排除されるわけである。ところが現実には、自殖率が高いのに近交弱勢も高い（有害遺伝子が多数保持されている）植物が非常に多い。この矛盾は今日まで、進化生物学における大きな謎とされている。本研究では、モデルを用いて、自殖集団においても劣勢有害遺伝子が排除されないメカニズムを提唱する。【モデルの仮定】胚発生の初期に関わる早期発現遺伝子と、以降の発達成長に関わる後期発現遺伝子とを考える。早期および後期発現の遺伝子に、劣勢の有害な遺伝子が生じる。胚珠は余剰生産されており、早期発現の有害遺伝子がホモとなり死亡した胚がある場合、その替わりに他の胚が発生する。発達した種子は、次世代に向けて生存競争を行う。後期発現の有害遺伝子がより多くホモとなっている種子ほど生存率が低く、この競争において不利である。【結果】シミュレーションの結果、自殖率が高い集団においても、早期発現・後期発現の有害遺伝子が多数保持されることがわかった。これは、「早期発現ホモかつ後期発現ホモ」の胚があらかじめ排除され、他の胚が種子に発達するためである。結果として、早期および後期の有害遺伝子をヘテロで持つ胚が発達しやすくなり、集団中から排除されにくくなる。さらには、有害遺伝子をヘテロで持つ種子が多くなるため、有害遺伝子を多数保有しながら生存率は高い種子が多くなる。そのため、集団の絶滅率も低くなるであろう。【結論】早期発現の有害遺伝子が「適応的」に働いて後期発現の有害遺伝子ホモ胚を排除するので、結果として、早期および後期発現の有害遺伝子が保有されることになる。
Conservation, taxonomy and ecology of the plants specific to reservoir environments.

MAKI MASAYUKI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2019/04/01 - 2022/03/31

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Reservoirs used for rice cultivation are drained during the winter, providing a unique habitats for plants for a short period of time. The existence of plant groups that are considered to be adapted to such environments has been recognized relatively recently. The objective of this study was to collect basic information to conserve such unique flora. The plants found specific to reservoirs include both species that are clearly differentiated from the species found in non-reservoir environments and those that are not. Those species tend to have high phenotypic plasticity and are well adapted to the special environment of post-drainage reservoirs. There found genetically differentiated and non-differentiated species among the plants specific to reservoirs environments.
Application of environmetal DNA methods to detect aquatic plants

Masayuki Maki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Tohoku University

2017/06/30 - 2021/03/31

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Although there are many rare species in aquatic plants, it is not easy to identify them. However, there is a possibility that the environmental DNA method, which has been rapidly developed in recent years, can be used to detect aquatic plants in water samples taken from surveyed areas. The purpose of this study was to establish a method to apply environmental DNA to the detection of aquatic plants and to investigate its applicability in lakes and ponds in wild. Chloroplast DNA is often used for plant DNA barcoding, but the water in lakes and ponds contains a large number of algae, which can also be detected. In this study, we developed PCR primers to detect vascular plants efficiently. When we applied this method to the field lake and ponds, we were able to detect vascular plants, including those that were actually growing in the lakes and ponds.
Reproductive interference in geographic cline of corolla tube length in Isodon umbrosus

Dohzono Ikumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tokyo Gakugei University

2017/04/01 - 2020/03/31

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We investigated whether reproductive interference between different species resulted in the geographic cline of corolla length of Isodon umbrosus. Isodon trichocarpus with very short corolla coexists with I. umbrosus with short corolla, and they shared a common bumblebee pollinator, Bombus honshuensis. In sympatric site, fertilization of I. umbrosus was reduced by pollen deposition from I. trichocarpus, suggesting reproductive interference between species. Precedence of conspecific pollen was detected in sympatric populations in I. trichocarpus, suggesting character displacement in pollen-pistil interactions. In addition, we found two genetic groups in I. umbrosus, suggesting that a historical process, the secondary contact between two diverged populations, caused geographic cline of corolla length.
How to avoid self-fertiliization: clarifying its evolution

Sakai Satoki, Itagaki Tomoyuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2016/04/01 - 2019/03/31

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To avoid self-fertilization, two types of self-incompatibilities, herkogamy, and dichogamy have evolved. I examined the evolution of these strategies, particularly examining self-incompatibilities. I proposed a new hypothesis that self-incompatibilities have evolved not to avoid self-fertilization but to avoid kin-fertilization.
Studies on ecotypic speciation mechanism in plants: Comparative genomic analysis in Solidago virgaurea group

Ito Motomi, Ishikawa Naoko, Nishino Takako

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: The University of Tokyo

2016/04/01 - 2019/03/31

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We have read total genome sequences for the purpose to clarify the mechanism of speciation for the Solidago which has undergone large-scale ecological speciation in the Japanese archipelago. Then, resequencing was performed for each ecotype, and mapping and mutation detection analyses were performed. As a result, it was revealed that the genetic differentiation of ion transporters such as Mg2 +, K +, NO3- and heavy metals, and flowering related genes is high between populations of serpentine soil and non-serpentine soil. On the other hand, in the comparison of alpine type and forest floor type, the degree of differentiation of flowering genes is low, and candidate genes found from serpentine types may not be involved in early-flowering of alpine type.
Conservation genetics of the rare plants emerging after the 2011 huge tsunami

MAKI MASAYUKI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2016/04/01 - 2019/03/31

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The tsunami following the Great East Japan Earthquake in 2011 caused extensive damage and heavily disturbed ground surfaces in north‐eastern Japan, severely affecting the biota of the area. Unexpectedly, many plant species established new populations in the tsunami inundation areas.The species newly emerging in the disturbed areas included several endangered species. In this study, we examined the genetic diversity and genetic differentiation among the populations of the rare plant species emerging newly after the 2011 tsuhami. In Eleocharis parvula, some populations emerging after the tsunami in Tohoku showed genetic similarity to those persisting before the tsunami while other populations emerging after tsunami showed genetic uniqueness. The former populations are considered to have originated from other persisting populations by long dispersal while the latter ones may have established from seed bank formed in the past. We also examined the genetic diversity in a few other species.
Genetic disturbance of native plants by alien species examined by specimens in herbaria

Maki Masayuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2015/04/01 - 2018/03/31

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In general, it is difficult to examine past ecological events, but specimens deposited in herbaria would be useful for such study because the specimens keep their DNA as when they were collected. These materials can be used to examine past hybridization between indigenous plant species and invasive alien species. We examined the possible values of the specimens in herbaria for study on past natural hybridization between native and invasive plants. In addition, field surveys and molecular genetic studies on on-going hybridization between two Rumex species, of which one is native and other is exotic, were conducted.
A mechanism in the maintenance of androdioecy from the view of a sex-specific marker transmission

MIYAKE Takashi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Gifu University

2015/04/01 - 2018/03/31

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Using NGS, we developed a sex-specific marker for androdioecious Schizopepon bryoniaefolius. This can be used in the reproductive stage of heterozygotic males, although there is a restriction in using in natural populations. From the view of the transmission advantage, we investigated the ratio of a sex-specific marker in pollen grains of heterozygotic males. Because of large differences among individuals, the ratio was not deviate from 1:1. However, it may imply that there remains a possibility that some males produce more pollen with the male-determinant gene. We did not find significant differences between sexes in pollen germination rate, growth rate, and survival rate.
Study on the scientific reference based on the aquatic plants of Nymphoides peltata

Fujii Shinji

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: University of Human Environments

2014/04/01 - 2018/03/31

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Genetic diversity of Nymphoides peltata, an aquatic plant widely distributed in Japan except Hokkaido, was very low and had no geographical structure of genetic variation. Each of the two populations of Notogawa in Lake Biwa and Tomitahama in Lake Kasumigaura possessed the greater part of the genetic diversity in Japan. In the view point of complimentary, conserving one of the two populations above with a population in Kahokugata or a population in Saga Plain could be effective for keeping the genetic diversity. In Lake Kasumigaura, only small area of the communities of floating plants was found based on the photos taken by US army in 1947. Nymphoides peltata grows on the wet land habitats both natural and cultivated and supposed to greatly change its growing places and distribution ranges in short period under the human activities.
Ecological differentiation in plants via character displacement avoiding reproductive interference

Dohzono Ikumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tokyo Gakugei University

2014/04/01 - 2017/03/31

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We analysed reproductive isolation, reproductive interference, and character displacement in Isodon umrosus with the short corolla and I. effusus with the long corolla. Artificial pollination between two species revealed that seed production and seed germination were very low, suggesting that hybridization between these two species is maladaptive. Seed production of wild plants in sympatric population was lower than that in allopatric population, indicating that the reproductive interference between the species occur in the field. In a sympatric site (Kanoto-iwa), flowering phenology differed between the species, which may play a role in avoiding their reproductive interference. The molecular phylogenetic analysis based on nuclear DNA sequences supported the previous taxonomic treatment of the genus at the species level.
Two problems in the evolution of self-incompatibility:theoretical and empirical studies

Sakai Satoki, MAKI MASAYUKI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2013/04/01 - 2016/03/31

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We modeled the initial invasion of the first pollen and pistil alleles in gametophytic SI to determine whether these alleles can stably co-exist in a population without tight linkage. We assume pollen and pistil loci each carry an incompatibility allele S and an allele without an incompatibility function N. We assume that pollen with an S allele are incompatible with pistils carrying S alleles, whereas other crosses are compatible. Ovules in pistils carrying an S allele suffer viability costs because recognition consumes resources. We found that the cost of carrying a pistil S allele allows pollen and pistil S alleles to co-exist in a stable equilibrium if linkage is partial. This occurs because parents that carry pistil S alleles but are homozygous for pollen N alleles cannot avoid self-fertilization; however, they suffer viability costs. Hence, pollen N alleles are selected again.
Genetic pollutin of Rumex species indigenous to Japan by alien invaders

Maki Masayuki, SHINJI FUJII, FUMIKO ISHIHAMA, SATOKI SAKAI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2012/04/01 - 2016/03/31

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Recent human activity enabled wild plants to move from their indigenous places to other areas in which they are not distributed naturally. Such alien species may hybridize with native congeners, resulting in genetic pollution in gene pools of the latter species. In this study, I investigated natural hybridization between Rumex species native to Japan and their congeneric alien species using flow cytometry and genetic markers. Based on previous morphological observations, it is known that Rumex longifolius, native species in Japan, often hybridized with the alien species R. obtusifolius and R. crispus. I certified the natural hybridization between R. longifolius and the two alien species by FCM and population genetic analyses. The data suggested that, in addition to F1 hybridization, back-crossings and F2 or later hybridization may have occurred between the native and the alien species. It is very concerned that genetic pollution of the native Rumex by the alien congeners occurs.
Phylogeography of "wind-hole plants": finding footprints of glacier era in lowland.

MAKI Masayuki, YAMASHIRO Tadashi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2012/04/01 - 2015/03/31

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Temperatures of areas around wind-holes are maintained to be very low even in summer. Therefore, plant species, which normally occurs in area at more high latitude or altitude, are uniquely found near wind-holes. It is generally considered that such unique plants (hereafter, wind-hole plants) once widely spread in the Japanese Archipelago during past colder ages but retreated to high latitude or altitude area although some populations near wind-holes are maintained after climate warming after the last glacier era. Thus, wind-hole areas may be refugia for wind-hole plants. In this study, the genetic differentiation between populations near wind-hole areas and in higher latitude/altitude areas in wind-hole plants were examined using molecular genetic markers to test the hypothesis above. There was no distinct difference in genetic constitution between wind-hole area populations and higher latitude/altitude ones, suggesting that wind-hole area may not be refugia for wind-hole plants.
Ecological and genetic differentiation among parapatric populations of bumblebee-pollinated plants.

DOHZONO Ikumi, MAKI Masayuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tokyo Gakugei University

2011 - 2013

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The corolla tube length of Isodon shikokianus shows geographical variation, corresponding with the proboscis length of two bumblebee pollinator species. We hypothesized that altitudinal variation in the pollinator assemblage is a principal factor mediating morphological and genetic differentiation among I. shikokianus populations. The differentiation in corolla tube length was explained by altitudinal difference. However, visitation frequency of two bumblebee species vary among years and pollination effectiveness do not depend on correspondence between the lengths of the flower corolla and the mouthparts of the bumblebees. Genetic distance was explained by altitudinal differentiation and relative frequency of two bumblebee species. These results suggest that floral and genetic divergence of I. shikokianus occurred in different altitude range, and the altitudinal cline of corolla tube length was a result of secondary contact between the long and short corolla populations.
Do natural hybridizations between the same species in different habitats lead to different evolutionary consequences?

MAKI Masayuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2009 - 2011

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Natural hybridization is not a rare phenomenon in plants. Interspecific hybridization is expected to occur in contact zones where two parental species co-occur. However such contact zones may not be similar in environmental condition, leading to different evolutionary consequences because the different environmental conditions likely influence the fitness of the individuals originated from natural hybridization even between the same species pairs. In this study, I addressed the question if natural hybridizations between the same species in different habitats lead to different evolutionary consequences. In a few hybridizing species pairs examined, gene flow patterns between the species were largely different among the populations. The patterns in phenotypic constitution in populations were also largely different among the populations. These differences may suggest that the hybridization between the different habitats results in different evolutionary consequences.
A basic research for conservation of endangered plant, Petrosavia sakuraii

TAKAHASHI Hiroshi, TOBE Hiroshi, YUKAWA Tomohisa, MAKI Masayuki, TSUDA Satoshi, IWASHINA Tsukasa, YAMATO Masahide

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Gifu University

2009 - 2011

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Pertrosavia sakuraii was a myco-heterotrophic plant associating with AM fungi of the genus Gromus. The plants grew in the fairly acid and moist soil on dark floor of Camaecyparis obtusa forests in landscape in central Japan, though they were few where many broad-leaved trees invaded. We also elucidated that development of the reproductive organs and embryo, movement of the subterranean stems, pollinators, seed germination characteristics, pollen-ovule ratios, inbreeding depression and phenology of P. sakuraii
Morphological, ecological and genetic study on the differentiation in the plants endemic to the Izu Islands

MAKI Masayuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2007 - 2008
Phylogenetical analysis of plants which have diverged rapidly based on nucleotide variations in nuclear genes

MAKI Masayuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2004 - 2005

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Phylogenetical analyses of the genus Hosta (Liliaceae) and Isodon (Labiatae) were conducted based on nucleotide variations in nuclear genes. Both Hosta and Isodon are considered to be have diverged rapidly in Japan. For Hosta, I determined a total of ca. 2300 bases of two alcohol dehydrogenase genes (Adh-land Adh-2) and a glyceraldehyde dehydrogenase (Gap). Based on the data, I carried out phylogenetical analyses. Compared to chloroplast DNA variation, many variations are found in these regions. Thus, it became clear that these nuclear genes have more phylogenetical information than cpDNA has. Most of genes cloned from a same taxon formed monophyletic groups, although some exceptions are found. Natural hybridizations or lineage sorting seems to relevant to this phylogeneical incongruity. For Isodon, I determined the nucleotide sequences in cpDNA, 18S-26S ITS, and 5S ITS. In cpDNA phylogeny, many incongruities were found, although such incongruities were less frequently found in nuclear DNA phylogeny. Natural hybridization seems to be involved in the divergence of the genus.
Mechanisms of reproductive isolation between two naturally hybridizing Epimedium diphyllum and E. sempervirens (Berberdiaceae)

MAKI Masayuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2001 - 2002

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Reproductive isolation between two naturally hybridizing Epimedium diphyllum and E. sempervires (Berberidaceae) was examined. In field observation, seasonal isolation of flowering was very weak, although E. sempervirens flowered slightly earlier than R diphyllum. The seasonal isolation does not seem to work for reproductive isolation between the two hybridizing species. In artificial crossing experiments, percentage of pollen germination was not different between intra-and interspecific crossing. Pollen tube growth was also not significantly different between intra-and interspecific crossing, although pollen tube growth in intraspecific crossing slightly larger than that in interspecific crossing. Thus, stage of pollen germination and pollen tube growth also does not seem to work for reproductive isolation between the two species. In addition, molecular phylogenetic analyses were done for the Japanese Epimedium. In the phylogenetic tree of chloro plast DNA, E. sempervkens and E. diplyllum belongs to different clades, suggesting that these two species contacted secondary and form the hybrid zone. In the phylogenetic tree of ITS and ETS region of 18S-26S rDNA, the phylogeny of Epimedium species was not fully solved due to scarcity of variation of these regions.
種分化による多様性創出のメカニズムの解明

河田雅圭, 横山潤, 牧雅之, 千葉聡, 久保田耕平, 曽田貞滋

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2000 - 2000

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生殖隔離がどのようなメカニズムで進化したかという問題の解明は、地球上での多様性創出の原因を考える上でも重要なテーマの一つである。この問題を「種分化のメカニズムの解明」と「種分化と生物の多様性の関係」から探る研究を押し進めるために、2回の会合を東北大学(仙台)で開催した(11月と2月)。会合では、植物、動物、微生物の対象生物について、理論的研究、分子生物学的研究、生態学的研究、生物地理学的研究、古生物学的研究について討論が行われた。討論の結果、種分化メカニズムの解明のための研究には、(1)交配や資源利用にかかわる遺伝メカニズムを分子レベルで解明する必要性であること、(2)自然界での多様性のプロセスを解明するためには、グローバルな視点からの多様性のパターンの解析が必要であり、そのためには個体をベースとたデータベースが必要であるということ、(3)どのように生態的な要因が種分化の促進に関わっているかを実証研究すること、(4)実際の生物に応用できる理論的研究の発展がのぞましいこと、などが確認された。また、今後の新たな研究計画に利用できるように、種分化研究の文献データベースの作成や一部計測データのデータベースの作成を行った。今回の成果は、日本生態学会でのシンポジウム「生物多様性の進化機構の解明」などで公開されるほか、雑誌での特集記事として企画している。また、継続的に論議し、今後、大きなプロジェクト研究として計画予定である。
野生植物における組み換え種分化の分子分類学的再検討

牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 奨励研究(A)

Institution: 東北大学

1998 - 1999

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組み換え種分化によって生じたと考えられる2種の野生植物,エヒメテンナンショウ(Arisaema ehimense)とヒメイカリソウ(Epimedium trifoliatobinatum)が,交雑起源であるかどうかを分子マーカーを用いて検討した。エヒメテンナンショウとその推定母種2種の複数集団からサンプリングを行い,酵素多型解析を行った。エヒメテンナンショウには,推定母種に見られないようなユニークな対立遺伝子はほとんど見られなかったことから,この種が交雑起源である可能性は高い。ただし、母種に特異的なマーカーが見つからなかったことから,雑種起源以外の仮説を完全に排除することはできない。ヒメイカリソウについては,RAPD(Random Amplified Polymorphic DNA)を用いて,交雑起源の検討を行った。ヒメイカリソウとその推定母種2種の集団から複数個体からサンプリングを行い,複数のランダムプライマーを用いてPCRを行い,各個体の遺伝子型を決定した。RAPDでは酵素多型を用いる場合よりも高い変異を検出することができる。ヒメイカリソウでは,母種に見られないバンドが検出された。これは,ヒメイカリソウが交雑によって起源してから長い時間が経っている可能性,ヒメイカリソウが交雑起源ではない可能性,RAPDの突然変異率が極端に高い可能性の3つを示唆しているが,そのどれであるかは今回の研究からだけでは明らかにすることができない。今後,葉緑体DNAや核DNAのITS領域の塩基配列変異を解析することによって,これらの仮説のどれが正しいかを明らかにすることが出来ると思われる。
草本植物における遺伝的変異・サイズ構造と多様性

原登志彦, 牧雅之, 鈴木和雄, 鈴木準一郎, 堀良通

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 北海道大学

1997 - 1998

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平成9-10年度の5月から9月にかけて以下の調査地で研究を行った。調査した植物材料はすべてマルハナバチ媒花である。 (1)北海道および九州:多年生草本コバノギボウシのサイズ、マルハナバチの訪花パターン、種子生産量の測定 (2)奥多摩:一年生草本ママコナの生長、種子生産量の測定 (3)北陸から山陰地方:多年生草本イカリソウの生長パターン(株立ちタイプと地下茎伸長タイプ)の調査 (4)会津田島:多年生草本クルマバハグマの生長、種子生産量の測定以上の調査地では各個体から葉のサンプリングも行い、酵素多型やDNAを用いて集団の遺伝的構造の解析を行った。生長パターンや種子生産量といった生態的特性に遺伝的特性がどの程度関係しているのかを解析した。また、コバノギボウシとイカリソウに関しては、広範囲の地域における調査とサンプリングを行ったので、これらの種の生態的特性と集団の遺伝的構造の地理的分化の関係も解析した。これらのデータ解析に基づき、モデリングなどの理論的研究も行った。研究成果は、8編の投稿論文としてまとめた。そのうち、1編がすでに印刷され、3編が印刷中、残り4編は平成12年3月現在投稿中である。
Evolutionary Ecology of Sex

YAHARA Tetsukazu, KOBAYASHI Ichizo, ONOSATO Hiroshi, TERACHI Toru, SASAKI Akira, IWASA Yoh

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: KYUSHU UNIVERSITY

1996 - 1998

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Adaptive significance of sexual reproduction remains uncertain. One of the purposes of this project was to unravel this mystery. Pathogen-driven selection has been regarded as a likely factor that can provide a general advantage for sexual reproduction (Red Queen hypothesis). T.Yahara tested this hypothesis using Eupatorium-geminivirus system by comparingsequence divergence of ORF 04, a host-range determinant of geminivirus between viruses infecting sexual and asexual host populations. Frequency of amino acid replacements was significantly higher in sexual host populations, supporting the hypothesis. A.Sasaki also developed a new model for gene-for-gene interactions between hosts and parasites and showed that antagonistic coevolution between hosts and parasites conserve fitness variation and make sexual reproduction advantageous. I.Kobayashi demonstrated that restriction-modification systems are parasites of bacterial genome and developed a new hypothesis that homologous recombination evolved as an counter-adaptation against gemomic parasites. Another target of this project was to describe some genetic systems under genetic conflicts and elucidate dynamics and outcomes of genetic interactions between genetic elements with conflicting interests in their transmission. Under suxual reproduction, Mendelian inheritance is a rule, but this rule is often violated by selfish behaviours of cytoplasmic genetic elements. In Raphanus, for example, male sterility is induced by a mitocondrial gene, but is restored by a nuclear gene. Outcomes of this interaction was examined in the field. Sex ratio distortion system in azuki-bean beetles, that is caused by Infection of microorganisms, was examined and complete nuclear control of sex ratio was confirmed in spite that infection of microorganism was very high. We also studied various phenomena of genomic imprinting. Y.Iwasa developed several models which can explain some unique properties of genomic inprinting.
Biological interactions on Primula sieboldii

WASHITANI Izumi, TOQUENAGA Yukihiko, MAKI Masayuki, SUZUKI Kazuo, KATO Makoto, OGUSHI Takayuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: University of Tsukuba

1996 - 1998

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Seed set and various biological and other environmental factors potentially attecting reproductive success were compared among 20 P.sieboldii local populations in a fragmented landscape of Hidaka region, southern Hokkaido and also in a metapopulation of a mountainous region in central Honshu . Although various biological agents including herbivores and pathogens affected the fertility of the plants, seed set of the populations was strongly dependent on the population size and pollinator availability which can be assessed by craw marks left on the flower petals by long-tongued bumblebee queens. Among the 20 populations investigated in Hidaka region, negligible seeds were set in smaller populations consisting of less than four genets, while in larger populations, population mean seed set strongly depended on pollinator availability especially in the year with generally low pollinator activities. Not only quantity of the seeds, but also their quality, i.e., fitness of the seeds which were barely produced on isolated mother plants of P sieboldii are also significantly lower than those of the seeds from less isolated mother plants having potential mating partner(s) in their vicinity.
キク科カワラノギクの集団構造に関する分子分類学的研究

牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 奨励研究(A)

Institution: 福岡教育大学

1996 - 1996

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絶滅危惧植物であるキク科カワラノギクの集団遺伝構造を明らかにすることを目的として、葉緑体DNAの遺伝子間領域及びイントロンにおける変異を解析した。カワラノギクの分布する複数の水系に属する集団から、1集団当たり数個体から数十個体の個体の成葉をサンプリングした。サンプリングした成葉からCTAB法を用いて、トータルDNAを抽出し、これをテンプレートDNAとして用いた。増幅を行った領域は、主に葉緑体DNA上のtrnT(UGU)とtrnL(UAA)の遺伝子間領域、trnL(UAA)とtrnF(GAA)の遺伝子間領域およびtrnL(UAA)のイントロンで、これ以外に一部のサンプルについてはtrnH(GUG)とtrnK(UUU)の遺伝子間領域についても増幅を行った。得られた増幅断片の長さにおいては、集団内および集団間で変異が見られなかった。また、得られた増幅断片を、複数の4塩基配列認識酵素で処理して、制限酵素断片長多型(RFLP)の検出を試みたが、集団内、集団間ともに変異は検出できなかった。以上の処理に加えて、DGGE(Denaturing Gradient Gel Electrophoresis)法によって、最小1塩基における置換を検出することを試みたが、今回増幅を行った断片については多型を検出することができなかった。これまでの葉緑体DNAの種内多型解析では、少ないながらも多型が検出されていることが多いことから考えても、カワラノギクが分化する際に強いピン首効果を受けたことによる影響が示唆される。現在、これらの増幅断片に本当に多型が存在しないのか、あるいはDGGE法における実験条件に問題があるのかを検討するために、DNAオートシーケンサーを用いて増幅断片の塩基配列を完全に決定し、多型の存在を確認する作業を進めている。
カワラナデシコにおける雌性両性花異株の進化と両性個体の自家交配の関係に関する研究

牧雅之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 奨励研究(A)

Institution: 福岡教育大学

1995 - 1995

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1.雌性両性花異株であるカワラナデシコの関東・中部地方の7集団について、両性個体の自家交配率を複数の酵素多型遺伝子座を用いた多重遺伝視座推定法で推定した。調査対象とした集団の雌性個体の頻度は約5%から約50%までの大きな変異を示していた。 2.カワラナデシコの両性個体は雄性先熟であるため、自家交配は起きにくいと予測されたが、ある程度の割合で自家交配が起きているこが確認された。この理由としては、同花受粉が起きている可能性と隣花受粉が起きている可能性の両方が考えられる。カワラナデシコは、最盛期には同一個体内で複数の花が同時に咲き、訪花昆虫は位置的に近い花を順々に訪れる傾向があるため隣家受粉が起こりうる。また、雄性先熟ではあるが、袋かけをして、訪花昆虫を排除してやっても、種子を生産しうるので、同花受粉も可能である。どちらの受粉様式が自家交配の主な要因となっているかは今後の課題である。 3.集団における雌性個体の頻度と両性個体の自家交配率との間には、強いとはいえないものの、相関関係が見られた。これは近交弱勢や両性型間での種子生産量には集団間で大きい違いがないものと仮定すれば、理論的に予測される結果と一致する。今後、近交弱勢や種子生産量の相対比の集団間の違いがどの程度であるかを推定する必要がある。

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