Details of the Researcher

PHOTO

Nobuhiro Suzuki
Section
Graduate School of Agricultural Science
Job title
Professor
Degree
Profile

Dr. Nobuhiro SUZUKI received his M.S. degree in phytopathology in 1985 and his Ph.D. degree in virology in 1989 from Tohoku University. Dr. Suzuki currently serves as a full professor at the Innovation Center for Future Agricultural Production and Food Industry, Graduate School of Agricultural Science, Tohoku University, and as an editor or editorial board member of Virus Research, Frontiers in Virology, mBio, Biology, and Journal of Virology. He has also served as a guest editor for PLoS Pathogens and Proceedings of the National Academy of Sciences.

The Suzuki Laboratory (Neovirology Laboratory) focuses on the characterization of diverse viruses infecting phytopathogenic fungi and on elucidating their interactions with their hosts. Recent achievements include the discovery of a neo-virus lifestyle exhibited by both a positive-sense single-stranded RNA virus and an unrelated double-stranded RNA virus in a plant-pathogenic fungus. Prior to moving to Sendai, Miyagi Prefecture, Dr. Suzuki served for 25 years as a professor and associate professor at the Institute of Plant Science and Resources (formerly the Institute of Plant Sciences and BioResources), Okayama University, beginning in 2001. Before joining Okayama University, he was a visiting fellow at the Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, from 1997 to 2001, where he studied the molecular biology of hypoviruses in the laboratory of Donald L. Nuss. Prior to his stay at UMBI, he served as an assistant professor and later as a lecturer at the Biotechnology Institute of the Akita Prefectural College of Agriculture from 1988 to 1998, where he conducted research on the molecular characterization of rice dwarf phytoreovirus, a member of the family Reoviridae. He has received awards from the Phytopathological Society of Japan and the Japanese Society for Virology in recognition of his outstanding contributions to plant virology.

Research History 9

  • 2025/10 - Present
    Graduate School of Agricultural Science, Tohoku University　Neovirology Laboratory, Innovation Center for Future Agricultural Production and Food Industry　Distinguished Professor

  • 2007/04 - 2026/03
    Institute of Plant Science and Resources　Group of Plant/Microbe Interactions　Professor

  • 2019/06 - 2019/11
    Swiss Federal Research Institute WSL　Forest Health & Biotic Interactions　WSL Fellow

  • 2001/04 - 2007/03
    Research Institute for Bioresources　Group of Plant/Microbe Interactions　Associate Professor

  • 1998/04 - 2001/03
    University of Maryland Biotechnology Institute　Center for Agricultural Biotechnology　Visiting Associate Professor

  • 1994/04 - 1998/03
    Akita Prefectural College of Agriculture　Biotechnology Institute,　Lecturer

  • 1996/04 - 1997/03
    University of Maryland Biotechnology Institute　Center for Agricultural Biotechnology　Visiting Scientist

  • 1994/04 - 1996/03
    National Agriculture Research Organization　Visiting Scientist

  • 1988/05 - 1990/03
    Akita Prefectural College of Agriculture　Biotechnology Institute　Assistant Professor

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Education 4

  • Tohoku University

    - 1989

  • Tohoku University　Graduate School, Division of Agriculture

    - 1989

  • Tohoku University　Faculty of Agriculture

    - 1983

  • Tohoku University　Faculty of Agriculture　Agronomy

    - 1983

Committee Memberships 38

  • mBio　Editor

    2025/07 - Present

  • International Committee on Taxonomy of Viruses (ICTV)　ICTV Executive Committee Member

    2020/10 - Present

  • Biology -Basel-　Editor

    2019/01 - Present

  • PLoS Pathogens　Guest Editor

    2017/01 - Present

  • Journal of Virology　Editorial Board Member

    2016/01 - Present

  • Frontiers in Virology　Editor

    2011/01 - Present

  • Virus Research　Editor

    2010/12 - Present

  • Virology　Editorial Board Member

    2015/01 - 2025/12

  • Proceedings of the National Academy of Science, U. S. A.　Guest Editor

    2014/01 - 2015/06

  • New Plant Protection　Editorial Board Member

    2025/07 - Present

  • Viruses　Editorial Board Member

    2022/09 - Present

  • Editor　Archives of Virology

    2022/01 - Present

  • The Japanese Society for Virology　Board of Directors

    2022/01 - Present

  • ICTV Study Group of the family Totiviridae　Study Group Chair

    2020/01 - Present

  • The Japanese Society for Virology　Board Member

    2015/01 - Present

  • ICTV Study Group of the family Chrysoviridae and Quadriviridae　Study Group Member

    2012 - Present

  • The Phytopathology Society of Japan　Plant Virus Taxonomy Committee of the Phytopathology Society of Japan

    2025/01 - 2026/03

  • Virology Journal　Editor

    2019/01 - 2025/03

  • The Phytopathological Society of Japan　Plant Virus Taxonomy Committee Chair

    2018/04 - 2024/12

  • The Phytopathological Society of Japan　Board of Directors

    2018/01 - 2024/12

  • ICTV Study Group of the family Hypoviridae　Study Group Member

    2000/01 - 2024/12

  • The Phytopathological Society of Japan　PSJ Plant Virus Disease Committee Member

    2004/04 - 2024/03

  • ICTV Study Group of the family Reoviridae　Study Group Member

    2012/01 - 2023/12

  • Journal of General Plant Pathology　sub-Editor-in-Chief

    2020/01 - 2021/12

  • Journal of General Plant Pathology　Editor

    2016/01 - 2020/12

  • ICTV Study Group of the family Hypoviridae　Study Group Chair

    2015/01 - 2020/12

  • ICTV Study Group of the family Partitivirus　Study Group Member

    2015/01 - 2020/12

  • The Japanese Society for Virology　Scientific Program Committee Member

    2019/04 - 2020/03

  • Frontiers in Cellular and Infection Microbiology　Guest Editor

    2018/01 - 2019/12

  • The Japanese Society for Virology　Screening Committee Member

    2018/01 - 2019/12

  • The Japanese Society for Virology　Board of Directors

    2016/01 - 2019/12

  • The Phytopathological Society of Japan　Plant Virus taxonomy Committee Member

    2004/04 - 2018/03

  • mBio　Guest Editor

    2017/01 - 2017/12

  • The Japanese Society for Virology　Scientific Program Committee Member

    2013/04 - 2017/03

  • ICTV Study Group of the family Partitiviridae　Study Group Chair

    2011/01 - 2014/12

  • The Japanese Society for Virology　Editor to UIRUS

    2010/01 - 2013/12

  • 日本ウイルス学会　広報委員会委員

    2009 - 2012

  • Virus Research　Editorial Board Member

    2007/12 - 2010/11

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Professional Memberships 3

  • 日本植物病理学会

  • 日本ウイルス学会

  • アメリカウイルス学会

Research Interests 7

  • antiviral defense

  • biocontrol

  • plant virus

  • fungal virus

  • RNA silencing

  • ウイルス学

  • Virology

Research Areas 1

  • Environmental science/Agricultural science / Conservation science (plants) /

Awards 10

  1. Springer Nature Author Service Award 2026

    2026/05　Springer Nature

  2. Springer Nature Editorial Contribution Award 2026

    2026/05　Springer Nature

  3. The Eighth Kanemitsu Award for Distinguished Scientific Achievements

    2025/11　Okayama University Alumni

  4. Editor of Distinction Awards 2025

    2025/05　Springer Nature

  5. Rob Goldbach Lecuture Award

    2024/10　Wageningen University

  6. Sanyo-Shimbun Scientific Achievement Award

    2024/01　Sanyo-Shimbun

  7. Top Peer Reviewer Award

    2019/09　Publon (Web of Science Group)

  8. 学会賞

    2017/03　日本植物病理学会

  9. 日本ウイルス学会奨励賞

    1997

  10. 日本植物病理学会奨励賞

    1995

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Papers 200

  1. RNA editing of genomic neighbors controls antiviral response in fungi

    Shinji Honda, Ayumi Yokoyama, Nobuhiro Suzuki

    Cell Host & Microbe　2025/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.chom.2025.02.016 　

    ISSN： 1931-3128

  2. Argonaute-independent, Dicer-dependent antiviral defense against RNA viruses. International-journal Peer-reviewed

    Yukiyo Sato, Hideki Kondo, Nobuhiro Suzuki

    Proceedings of the National Academy of Sciences of the United States of America　121　(25)　e2322765121　2024/06/18

    DOI： 10.1073/pnas.2322765121 　

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    Antiviral RNA interference (RNAi) is conserved from yeasts to mammals. Dicer recognizes and cleaves virus-derived double-stranded RNA (dsRNA) and/or structured single-stranded RNA (ssRNA) into small-interfering RNAs, which guide effector Argonaute to homologous viral RNAs for digestion and inhibit virus replication. Thus, Argonaute is believed to be essential for antiviral RNAi. Here, we show Argonaute-independent, Dicer-dependent antiviral defense against dsRNA viruses using Cryphonectria parasitica (chestnut blight fungus), which is a model filamentous ascomycetous fungus and hosts a variety of viruses. The fungus has two dicer-like genes (dcl1 and dcl2) and four argonaute-like genes (agl1 to agl4). We prepared a suite of single to quadruple agl knockout mutants with or without dcl disruption. We tested these mutants for antiviral activities against diverse dsRNA viruses and ssRNA viruses. Although both DCL2 and AGL2 worked as antiviral players against some RNA viruses, DCL2 without argonaute was sufficient to block the replication of other RNA viruses. Overall, these results indicate the existence of a Dicer-alone defense and different degrees of susceptibility to it among RNA viruses. We discuss what determines the great difference in susceptibility to the Dicer-only defense.

  3. Replication of single viruses across the kingdoms, Fungi, Plantae, and Animalia. International-journal Peer-reviewed

    Paul Telengech, Kiwamu Hyodo, Hiroaki Ichikawa, Ryusei Kuwata, Hideki Kondo, Nobuhiro Suzuki

    Proceedings of the National Academy of Sciences of the United States of America　121　(25)　e2318150121　2024/06/18

    DOI： 10.1073/pnas.2318150121 　

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    It is extremely rare that a single virus crosses host barriers across multiple kingdoms. Based on phylogenetic and paleovirological analyses, it has previously been hypothesized that single members of the family Partitiviridae could cross multiple kingdoms. Partitiviridae accommodates members characterized by their simple bisegmented double-stranded RNA genome; asymptomatic infections of host organisms; the absence of an extracellular route for entry in nature; and collectively broad host range. Herein, we show the replicability of single fungal partitiviruses in three kingdoms of host organisms: Fungi, Plantae, and Animalia. Betapartitiviruses of the phytopathogenic fungusRosellinia necatrix could replicate in protoplasts of the carrot (Daucus carota), Nicotiana benthamiana and Nicotiana tabacum, in some cases reaching a level detectable by agarose gel electrophoresis. Moreover, betapartitiviruses showed more robust replication than the tested alphapartitiviruses. One of the fungal betapartitiviruses, RnPV18, could persistently and stably infect carrot plants regenerated from virion-transfected protoplasts. Both alpha- and betapartitiviruses, although with different host preference, could replicate in two insect cell lines derived from the fall armyworm Spodoptera frugiperda and the fruit fly Drosophila melanogaster. Our results indicate the replicability of single partitiviruses in members of three kingdoms and provide insights into virus adaptation, host jumping, and evolution.

  4. Three-Layered Complex Interactions among Capsidless (+)ssRNA Yadokariviruses, dsRNA Viruses, and a Fungus Peer-reviewed

    Yukiyo Sato, Sakae Hisano, Carlos José López-Herrera, Hideki Kondo, Nobuhiro Suzuki

    mBio　2022/08/30

    Publisher: American Society for Microbiology

    DOI： 10.1128/mbio.01685-22 　

    eISSN： 2150-7511

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    A capsidless (+)ssRNA virus YkV1 (family Yadokariviridae ) highjacks the capsid of an unrelated dsRNA virus YnV1 (proposed family “ Yadonushiviridae ”) in a phytopathogenic ascomycete, while YkV1 trans -enhances YnV1 replication. Herein, we identified the dsRNA virus partners of three yadokariviruses (YkV3, YkV4a, and YkV4b) with genome organization different from YkV1 as being different from YnV1 at the suborder level.

  5. Mycovirus Diversity and Evolution Revealed/Inferred from Recent Studies. International-journal Invited

    Hideki Kondo, Leticia Botella, Nobuhiro Suzuki

    Annual review of phytopathology　2022/05/24

    DOI： 10.1146/annurev-phyto-021621-122122 　

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    High-throughput virome analyses with various fungi, from cultured or uncultured sources, have led to the discovery of diverse viruses with unique genome structures and even neo-lifestyles. Examples in the former category include splipalmiviruses and ambiviruses. Splipalmiviruses, related to yeast narnaviruses, have multiple positive-sense (+) single-stranded (ss) RNA genomic segments that separately encode the RNA-dependent RNA polymerase motifs, the hallmark of RNA viruses (members of the kingdom Orthornavirae). Ambiviruses appear to have an undivided ssRNA genome of 3∼5 kb with two large open reading frames (ORFs) separated by intergenic regions. Another narna-like virus group has two fully overlapping ORFs on both strands of a genomic segment that span more than 90% of the genome size. New virus lifestyles exhibited by mycoviruses include the yado-kari/yado-nushi nature characterized by the partnership between the (+)ssRNA yadokarivirus and an unrelated dsRNA virus (donor of the capsid for the former) and the hadaka nature of capsidless 10-11 segmented (+)ssRNA accessible by RNase in infected mycelial homogenates. Furthermore, dsRNA polymycoviruses with phylogenetic affinity to (+)ssRNA animal caliciviruses have been shown to be infectious as dsRNA-protein complexes or deproteinized naked dsRNA. Many previous phylogenetic gaps have been filled by recently discovered fungal and other viruses, which have provided interesting evolutionary insights. Phylogenetic analyses and the discovery of natural and experimental cross-kingdom infections suggest that horizontal virus transfer may have occurred and continue to occur between fungi and other kingdoms. Expected final online publication date for the Annual Review of Phytopathology, Volume 60 is August 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

  6. Proof of concept of the Yadokari nature: A capsidless replicase-encoding but replication-dependent positive-sense single-stranded RNA virus hosted by an unrelated double-stranded RNA virus Peer-reviewed

    Subha Das, Md Mahfuz Alam, Rui Zhang, Sakae Hisano, Nobuhiro Suzuki

    Journal of Virology　95　(17)　e00467-21　2021/09

    DOI： 10.1128/JVI.00467-21 　

    ISSN： 0022-538X

    eISSN： 1098-5514

  7. Establishment of Neurospora crassa as a model organism for fungal virology Peer-reviewed

    Shinji Honda, Ana Eusebio-Cope, Shuhei Miyashita, Ayumi Yokoyama, Annisa Aulia, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

    Nature Communications　11　(1)　5627　2020/12/01

    DOI： 10.1038/s41467-020-19355-y 　

    eISSN： 2041-1723

  8. Hadaka virus 1: A capsidless eleven-segmented positive-sense single-stranded RNA virus from a phytopathogenic fungus, Fusarium oxysporum International-journal Peer-reviewed

    Yukiyo Sato, Wajeeha Shamsi, Atif Jamal, Muhammad Faraz Bhatti, Hideki Kondo, Nobuhiro Suzuki, Reed B. Wickner

    mBio　11　(3)　e0045-20　2020/05

    DOI： 10.1128/mBio.00450-20 　

    ISSN： 2161-2129

    eISSN： 2150-7511

  9. Dicer functions transcriptionally and posttranscriptionally in a multilayer antiviral defense. Peer-reviewed

    Andika IB, Kondo H, Suzuki N

    Proceedings of the National Academy of Sciences of the United States of America　116　(6)　2274-2281　2019/02/05

    DOI： 10.1073/pnas.1812407116 　

    ISSN： 0027-8424

  10. SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing Peer-reviewed

    Ida Bagus Andika, Atif Jamal, Hideki Kondo, Nobuhiro Suzuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　114　(17)　E3499-E3506　2017/04

    DOI： 10.1073/pnas.1701196114 　

    ISSN： 0027-8424

  11. Harnessing host ROS-generating machinery for the robust genome replication of a plant RNA virus Peer-reviewed

    Kiwamu Hyodo, Kenji Hashimoto, Kazuyuki Kuchitsu, Nobuhiro Suzuki, Tetsuro Okuno

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　114　(7)　E1282-E1290　2017/02

    DOI： 10.1073/pnas.1610212114 　

    ISSN： 0027-8424

  12. A capsidless ssRNA virus hosted by an unrelated dsRNA virus Peer-reviewed

    Rui Zhang, Sakae Hisano, Akio Tani, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    Nature Microbiology　1　(1)　2016/01/11

    DOI： 10.1038/nmicrobiol.2015.1 　

    ISSN： 2058-5276

  13. Highly activated RNA silencing via strong induction of dicer by one virus can interfere with the replication of an unrelated virus Peer-reviewed

    Sotaro Chiba, Nobuhiro Suzuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　112　(35)　E4911-E4918　2015/09

    DOI： 10.1073/pnas.1509151112 　

    ISSN： 0027-8424

  14. Mycoreovirus genome rearrangements associated with RNA silencing deficiency Peer-reviewed

    Ana Eusebio-Cope, Nobuhiro Suzuki

    NUCLEIC ACIDS RESEARCH　43　(7)　3802-3813　2015/04

    DOI： 10.1093/nar/gkv239 　

    ISSN： 0305-1048

    eISSN： 1362-4962

  15. Virus Research Special Issue “Cross-kingdom infection by viruses” Invited Peer-reviewed

    Nobuhiro SUZUKI, Shin-Yi L. MARZANO, Taiyun WEI, Massimo TURINA

    Virus Research　370　199763-199763　2026/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.virusres.2026.199763 　

    ISSN： 0168-1702

  16. Tripartite Interaction Between Penicillium pinophilum‐ Host Plants and CMV‐Y: A Model for Endophyte‐Mediated Viral Biocontrol Peer-reviewed

    Sarah R. Ibiang, Ivan Galis, Hideki Kondo, Nobuhiro Suzuki

    Plant, Cell & Environment　49　70741　2026/07/17

    Publisher: Wiley

    DOI： 10.1111/pce.70741 　

    ISSN： 0140-7791

    eISSN： 1365-3040

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    ABSTRACT Plant‐fungus‐virus tripartite interactions represent complex ecological systems in which mutualistic endophytes can influence host physiology, yet the molecular basis of endophyte‐mediated defence remains poorly understood. Here, we demonstrate that the endophytic fungus Penicillium pinophilum EU0013 suppresses the yellow strain of cucumber mosaic virus (CMV‐Y) in Solanum lycopersicum and Nicotiana benthamiana . CMV‐Y infection induced pronounced oxidative and hormonal perturbations, which were mitigated by P. pinophilum colonisation. Endophyte‐associated plants exhibited early accumulation of jasmonate intermediates, consistent with the activation of jasmonate signalling. This response promoted the degradation of jasmonate‐ZIM‐domain proteins and release of core JA‐responsive transcription factors. Virus‐induced gene silencing identified MYC2 as a key regulator required for endophyte‐mediated antiviral protection, with WRKY17 contributing to defence modulation. In parallel, transcriptome analysis revealed the upregulation of a Dicer‐like gene, indicating enhanced engagement of RNA silencing, a primary antiviral mechanism targeting viral RNA. This coordinated activation occurred alongside significant induction of pathogenesis‐related proteins, particularly PR9, and improved control of reactive oxygen species. Salicylic acid‐responsive defences showed a host‐modulated pattern, with PR1 induced by CMV and PR2/PR5 preferentially enhanced in endophyte‐associated plants. Collectively, these findings demonstrate that P. pinophilum enhances antiviral immunity through coordinated defence integration pathways, providing a framework for endophyte‐based viral disease management.

  17. Toward in planta studies of persistent fungal viruses in a model plant. International-journal

    Paul Telengech, Sakae Hisano, Francesco Favaretto, Hiroaki Ichikawa, Kazuyuki Maruyama, Kiwamu Hyodo, Hideki Kondo, Nobuhiro Suzuki

    Virus research　199761-199761　2026/06/07

    DOI： 10.1016/j.virusres.2026.199761 　

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    A model plant, Nicotiana benthamiana, was examined as a host for persistent fungal viruses capable of crossing organismal kingdoms. Protoplasts of N. benthamiana were transfected with a mixture of virions of a betapartitivirus, Rosellinia necatrix partitivirus 18 (RnPV18), and an alphapartitivirus, RnPV19, and were then subjected to plantlet regeneration. Primary RT-PCR-based screening showed that nearly 100% of the resulting calli tested positive for RnPV18, whereas approximately 90% were positive for RnPV19. However, secondary screening performed at a later stage of tissue culture revealed that only 6% of the calli retained RnPV19, whereas approximately 33% retained RnPV18. These results suggest that the calli were chimeric, consisting of virus-infected and virus-free sectors, and that the partitiviruses were progressively lost during callus maintenance. It is also possible that these fungal partitiviruses were unable to fully adapt to, or counteract, host defense responses sufficiently to establish stable infection. We succeeded in obtaining RnPV18-positive calli and suspension cultures that maintained the virus at detectable levels, as shown by northern blotting, after prolonged subculture for at least 9 months. High-throughput small RNA analyses revealed both similarities and differences in virus-derived small RNA profiles among protoplasts, calli, and suspension cultures. Viral genome analyses further revealed developmental stage-specific and stage-independent substitutions compared with the RnPV18 genomic sequence maintained in the original fungal host. Interestingly, a C-to-U mutation in the RNA-dependent RNA polymerase-encoding region of RnPV18 was detected much more frequently in a particular line, designated B21, than in another stably RnPV18-infected line, P8, irrespective of whether the virus was maintained in suspension cultures or calli. This may explain why virus accumulation in B21 calli and suspension cultures was much lower than that in P8 cell cultures, as RNA-seq analyses showed 159 K counts per million for P8 versus 44 K counts per million for B21. Taken together, this study provides a platform for investigating partitiviruses and other ubiquitous persistent viruses, which are generally difficult to inoculate experimentally.

  18. Mycoviruses diversity in the black kōji mold, Aspergillus luchuensis (section Nigri) isolated from liquor-production environments in Japan. International-journal

    Hideki Kondo, Misaki Nanaji, Hitomi Sugahara, Miki Fujita, Ida Bagus Andika, Nobuhiro Suzuki, Fujimori Fumihiro

    Virus research　199724-199724　2026/04/04

    DOI： 10.1016/j.virusres.2026.199724 　

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    Some fungal species in the genus Aspergillus are economically important due to their role in the production of liquors and various foods; however, their viromes, which may affect their performance, remain unexplored. Therefore, this study examined the viromes of nine strains of Aspergillus luchuensis (section Nigri), the black kōji mold used in the production of shochu (a traditional Japanese liquor) in Japan. It identified virus-like sequences related to alterna-, partiti-, curvula, botourmia-, narna-like, and umbra-like viruses. Some sequences appear to represent new variants (e.g., alterna- and gammapartitiviruses), while many others correspond to novel viral species within established or proposed mycoviral families. All A. luchuensis strains harbored multiple virus infections, with 2 to 7 viruses per strain. Three alternavirus strains with four-segmented double-stranded RNA (dsRNA) genomes were confirmed, along with minor variants co-present with the predominant strains. Notably, a gammapartitivirus appears to have two additional dsRNA genome segments, along with two satellite-like short dsRNA segments in some fungal isolates. Furthermore, at least five short RNAs (0.48-1.31 kb) were identified, three of which are possibly satellite-like RNAs associated with novel single-stranded RNA viruses (botourmia- and umbra-like viruses). These findings reveal the great diversity of mycoviruses in A. luchuensis populations and lay the foundation for further investigation into their impact on fungal phenotypes and liquor production.

  19. 2b proteins of highly virulent cucumber mosaic virus strains are associated with suppression of DCL4 activity Peer-reviewed

    Toshiyuki Fukuhara, Shuhei Miyashita, Nobuhiro Suzuki, Hideki Takahashi

    Plant and Cell Physiology　67　(7)　1099-1110　2026/03/23

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/pcp/pcag036 　

    ISSN： 0032-0781

    eISSN： 1471-9053

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    Abstract RNA silencing constitutes a fundamental antiviral defense mechanism in plants. Although its role in virus resistance has been extensively studied, the relationship between the dicing activity of Dicer—an essential enzyme in RNA silencing—and antiviral defense remains unclear. To address this knowledge gap, we previously established a simple in vitro dicing assay that enables the direct assessment of the activities of two Dicers, DCL3 and DCL4. We hypothesized that this assay could reveal how viral infection modulates DCL3 and DCL4 activities. Here, we report a negative correlation between NbDCL4 activity and symptom severity using the Nicotiana benthamiana–cucumber mosaic virus (CMV) system. We demonstrate that NbDCL4 activity is specifically suppressed in plants infected with highly virulent subgroup I (sg I) strains of CMV. In vitro dicing assays and small RNA sequencing analyses further reveal that NbDCL4 activity toward long double-stranded RNA (dsRNA) remains unaffected, whereas its activity toward shorter dsRNAs (~50 nt) is markedly inhibited in CMV(sg I)-infected plants. Subsequent analyses using a 2b-deficient disarmed mutant and a less virulent subgroup II strain of CMV indicate that this inhibition is mediated by the sg I 2b protein, a viral suppressor of RNA silencing. Taken together, these findings provide mechanistic insights into the molecular basis of CMV virulence, suggesting that the severe symptoms induced by highly virulent CMV strains are, at least in part, attributable to 2b-mediated inhibition of DCL4 activity.

  20. Virome of the fungi associated with mushroom dry bubble disease. International-journal

    Lóránt Hatvani, Sakae Hisano, Hideki Kondo, Hitomi Sugahara, Paul Telengech, Sabitree Shahi, Sarah Remi Ibiang, Sándor Kocsubé, Tünde Kartali, David A Fitzpatrick, Helen Grogan, Nobuhiro Suzuki

    Virus research　367　199714-199714　2026/03/18

    DOI： 10.1016/j.virusres.2026.199714 　

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    Dry bubble disease, attributed to the filamentous fungus Lecanicillium fungicola (Cordycipitaceae) results in huge yield losses in mushroom (Agaricus bisporus) cultivation worldwide. The possibilities for controlling the disease using commercial fungicides are highly limited, and therefore, there is an increasing demand for novel, alternative means of pest management. Our research objective was the comprehensive examination of viruses in the causal agents of dry bubble disease, which may open up an avenue for its virocontrol in the future. Out of 57 fungal isolates obtained from dry bubble-affected A. bisporus crops in various countries, 47 (82%) were confirmed by ITS (Internal Transcribed Spacer) sequence analysis as L. fungicola. In addition, different members of the genera Akanthomyces and Simplicillium (7 and 3 isolates, respectively), yet unknown to cause dry bubble symptoms, have also been detected. Cellulose column chromatography revealed the presence of double-stranded (ds) RNA in seven L. fungicola and three Akanthomyces sp. isolates, suggesting viral infection. The ten dsRNA-positive and eight randomly selected dsRNA-negative fungal strains were subjected to rRNA-depletion high-throughput RNA-sequencing analysis. The presence of seven new viruses representing four new species in the established families, Partitiviridae, Polymycoviridae, Botourmiaviridae and the narna-like virus group, and three previously established/proposed species in the families Chrysoviridae and "Mycovirgaviridae" were confirmed. The impact of the detected and identified viruses on their host fungi, and their potential applicability for virocontrol purposes will be examined in the future. This study provides the first detailed report on viruses of mushroom pathogenic fungi.

  21. Core virome shapes adaptation of a phytopathogenic fungus to climate and cropping patterns Peer-reviewed

    Kang Zhou, Yue Deng, Chenghuan Zhu, Long Yang, Jing Zhang, Weidong Chen, Nobuhiro Suzuki, Guoqing Li, Mingde Wu

    The ISME Journal　20　(1)　2026/01/12

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/ismejo/wrag001 　

    ISSN： 1751-7362

    eISSN： 1751-7370

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    Abstract Despite extensive exploration of fungal viromes (mycoviromes), the ecological roles of mycoviruses remain poorly understood. Hence, we investigated the virome of Leptosphaeria biglobosa (an important fungal pathogen of rapeseed) from different geographic origins to determine the impacts of external factors on virome composition and their role in fungal ecological adaptation. The viromes of different L. biglobosa groups were investigated, and viral diversity correlated positively with field disease incidence and host diversity, but negatively with the altitude of the strain collection sites. A positive single-stranded RNA virus, namely, Leptosphaeria biglobosa letobirnavirus 1 (LbLV1), one of the core virome members (predominant viruses that constitute the majority of the viral community), has a significantly high incidence in L. biglobosa populations in winter rapeseed in southern China but a low incidence in L. biglobosa populations in spring rapeseed in northern China. Further laboratory and field tests revealed that LbLV1 could increase the ability of L. biglobosa to oversummer at average temperatures ranging from 23°C to 34°C in the winter rapeseed region of China. Therefore, the variation in LbLV1 incidence between winter and spring rapeseed should be a consequence of LbLV1-mediated adaptation to climate and cropping patterns. Furthermore, one gene, namely Lbhsp12, significantly induced by the hypothetical protein of LbLV1, is responsible for LbLV1-mediated thermal tolerance. Our findings indicate that mycovirome composition reflects environmental constraints, and core viruses can drive ecological adaptation by modulating host stress responses.

  22. “Tobaliviridae”, a new family of filamentous mycoviruses in the order Martellivirales

    Sead Sabanadzovic, Nina Aboughanem-Sabanadzovic, Massimo Turina, Nobuhiro Suzuki, Mart Krupovic

    Archives of Virology　171　(2)　2026/01/10

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-025-06500-5 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  23. Thorough characterization of a new curvulavirid from a Japanese strain of Cryphonectria nitschkei Peer-reviewed

    Sabitree Shahi, Sakae Hisano, Wasiatus Sa'diyah, Yoshihiro Takaki, Hideki Kondo, Nobuhiro Suzuki

    Journal of General Virology　106　(12)　002177　2025/12/17

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002177 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    A new curvulavirid was isolated from a Japanese strain of the filamentous ascomycete Cryphonectria nitschkei and thoroughly characterized. The virus termed Cryphonectria nitschkei curvulavirus 1 (CnCvV1) has a bi-segmented dsRNA genome. CnCvV1 dsRNA1 encodes an RNA-dependent RNA polymerase (592 amino acids), while dsRNA2 possesses two ORFs, one that encodes a protein associated with the genomic dsRNA and the other that encodes a hypothetical protein of unknown function. CnCvV1 could be experimentally introduced into another virus-free strain of C. nitschkei and two strains of different fungal species within the genus Cryphonectria ( Cryphonectria parasitica and Cryphonectria carpinicola ). Based on phenotypic comparison, the virus caused asymptomatic infection in the three newly established fungal strains. However, there was a reduced colony growth rate and increased CnCvV1 accumulation in an RNA silencing-deficient mutant (Δ dcl2 ), relative to the wt strain EP155 of a model virus host fungus ( C. parasitica ). These findings suggest that CnCvV1 is targeted by RNA silencing in C. parasitica . This study provides a foundation for further exploration of curvulavirids that have been biologically understudied.

  24. A Viral RNA Silencing Suppressor Modulates Reactive Oxygen Species Levels to Induce the Autophagic Degradation of Dicer‐Like and Argonaute‐Like Proteins Peer-reviewed

    Shiyu Zhai, Tianxing Pang, Shiyu Peng, Shenshen Zou, Zhiping Deng, Nobuhiro Suzuki, Zhensheng Kang, Ida Bagus Andika, Liying Sun

    Advanced Science　e06572　2025/09/08

    Publisher: Wiley

    DOI： 10.1002/advs.202506572 　

    ISSN： 2198-3844

    eISSN： 2198-3844

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    Abstract Mounting evidence indicates that viruses exploit elevated reactive oxygen species (ROS) levels to promote replication and pathogenesis, yet the mechanistic underpinnings of this viral strategy remain elusive for many viral systems. This study uncovers a sophisticated viral counter‐defense mechanism in the Cryphonectria hypovirus 1 (CHV1)‐Fusarium graminearum system, where the viral p29 protein subverts host redox homeostasis to overcome antiviral responses. That p29 directly interacts with and inhibits the enzymatic activity of fungal NAD(P)H‐dependent FMN reductase 1 (FMR1), leading to increased ROS accumulation and subsequent autophagy activation is demonstrated. Strikingly, this ROS‐induced autophagy selectively targets for degradation two core antiviral RNA silencing components against CHV1 in F. graminearum, Dicer‐like 2 (DCL2) and Argonaute‐like 1 (AGL1), thereby compromising the host's primary antiviral defense system. Genetic analysis confirms this coordinated hijacking of host machineries, as CHV1 shows enhanced accumulation in the FMR1 knockout and reduced accumulation in autophagy‐deficient fungal strains. This work reveals a tripartite interplay among oxidative stress, autophagy, and RNA silencing that CHV1 manipulates through p29 multifunctional activity. These findings provide a model for how viruses coordinately regulate distinct host defense systems to optimize infection.

  25. Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Archaeal Viruses Subcommittee, 2025 Invited Peer-reviewed

    Mart Krupovic, Diana P. Baquero, Eduardo A. Bignon, Ariane Bize, Guillaume Borrel, Mingwei Cai, Lanming Chen, Marion Coves, Changhai Duan, Simonetta Gribaldo, Eugene V. Koonin, Meng Li, Lirui Liu, Yang Liu, Sofia Medvedeva, Yimin Ni, Apoorva Prabhu, Christian Rinke, Yongjie Wang, Tianqi Xu, Shuling Yan, Qinglu Zeng, Rui Zhang

    Journal of General Virology　106　(7)　002117　2025/07/25

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002117 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    The International Committee on Taxonomy of Viruses (ICTV) holds a ratification vote annually following the review of newly proposed taxa by ICTV Study Groups and members of the virology community. This article reports changes to the taxonomy of viruses infecting archaea that were approved and ratified by the ICTV in March 2025. Six new families of head-tailed viruses expanded the order Caudoviricetes (realm Duplodnaviria); one new family of filamentous viruses was added to the order Ligamenvirales (realm Adnaviria); one new family of viruses with pleomorphic virions was included within a new phylum, new order and new class in the kingdom Trapavirae (realm Monodnaviria); finally, three new families were created for spindle-shaped viruses that remain unassigned to higher level taxa. The 25 new species represent viruses infecting a broad range of archaea, including members of the classes Archaeoglobi, Bathyarchaeia, Methanobacteria, Methanomicrobia, Nitrososphaeria and Poseidoniia. Most of these viruses have been discovered by metagenomics in samples derived from diverse environments, including ambient and extreme marine ecosystems, the gastrointestinal tract of humans and animals, anaerobic digesters and terrestrial hot springs. Following this taxonomic update, archaeal viruses are officially classified into a total of 163 virus species in 94 genera within 62 families.

  26. Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) – General taxonomy proposals, 2025 Invited Peer-reviewed

    F. Murilo Zerbini, Anya Crane, Jens H. Kuhn, Peter Simmonds, Elliot J. Lefkowitz

    Journal of General Virology　106　(7)　002116　2025/07/25

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002116 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    During the 56th annual meeting of the International Committee on Taxonomy of Viruses (ICTV), held in Bari, Italy, in August 2024, two technical proposals were presented. The first called for amended versions of accepted taxonomic proposals to be named in such a way to ensure that they are readily accessible on the ICTV website (2024.001G). The second proposed a substantial reformatting of the ICTV statutes and codes to produce a more unified text after the numerous changes made to both documents in previous years (2024.002G). Finally, the ICTV Executive Committee (EC) nominated Professor Stuart Siddell as a Life Member of the ICTV for his work over four decades on virus taxonomy, including 16 years as a member of the EC (2024.003G).

  27. Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Fungal and Protist Viruses Subcommittee, 2025 Invited Peer-reviewed

    Sead Sabanadzovic, Chantal Abergel, Marı́a A. Ayllón, Leticia Botella, Marta Canuti, Yuto Chiba, Jean-Michel Claverie, Robert H.A. Coutts, Stefania Daghino, Livia Donaire, Marco Forgia, Ondřej Hejna, Jichun Jia, Daohong Jiang, Ioly Kotta-Loizou, Mart Krupovic, Andrew S. Lang, Matthieu Legendre, Shin-Yi Lee Marzano, Luca Nerva, Judit Pénzes, Anna Poimala, Sofia Rigou, Yukiyo Sato, Wajeeha Shamsi, Nobuhiro Suzuki, Massimo Turina, Syun-ichi Urayama, Eeva J. Vainio, Jiatao Xie

    Journal of General Virology　106　(7)　002115　2025/07/25

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002115 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    The Fungal and Protist Viruses Subcommittee (SC) of the International Committee on Taxonomy of Viruses (ICTV) has received a total of eight taxonomic proposals for the 2024 annual cycle. The extent of proposed changes varied, including nomenclatural updates, creation of new taxa and reorganization of established taxa. Following the ICTV procedures, all proposals were reviewed and voted upon by the members of the Executive Committee with ratification in March 2025. As a result, a total of 52 species in the families Botourmiaviridae and Marnaviridae were renamed to comply with the mandated binomial format. A new genus has been added to the dsRNA virus family Amalgaviridae, while two new families, Splipalmiviridae (Wolframvirales) and Mycoalphaviridae (Hepelivirales), were created to classify new groups of positive-sense (+) RNA mycoviruses. The class Arfiviricetes (Cressdnaviricota) was expanded by a new order Lineavirales and a new family Oomyviridae of ssDNA viruses. Additionally, a new class Orpoviricetes was created in the kingdom Orthornavirae to classify a group of bisegmented (+)RNA viruses reported from fungi and oomycetes. Finally, the order Pimascovirales was reorganized to better depict evolutionary relationships of pithoviruses and related viruses with large dsDNA genomes. The summary of updates in the taxonomy of fungal and protist viruses presented here is limited to taxa within the remit of this Subcommittee. For information on taxonomy changes on other fungal viruses closely related to animal and/or plant viruses, please see reports from sister ICTV Subcommittees (i.e. Plant Virus SC and Animal dsRNA and ssRNA(−) Viruses SC).

  28. Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses from the Plant Viruses Subcommittee, 2025 Invited Peer-reviewed

    Luisa Rubino, Peter Abrahamian, Wenxia An, Miguel A. Aranda, José T. Ascencio-Ibañez, Nicolas Bejerman, Arnaud G. Blouin, Thierry Candresse, Tomas Canto, Mengji Cao, John P. Carr, Won Kyong Cho, Fiona Constable, Indranil Dasgupta, Humberto Debat, Ralf G. Dietzgen, Michele Digiaro, Livia Donaire, Toufic Elbeaino, Denis Fargette, Fiona Filardo, Matthias G. Fischer, Nuria Fontdevila, Adrian Fox, Juliana Freitas-Astua, Marc Fuchs, Andrew D.W. Geering, Mahan Ghafari, Anders Hafrén, John Hammond, Rosemarie Hammond, Beata Hasiów-Jaroszewska, Eugenie Hebrard, Carmen Hernández, Jean-Michel Hily, Ahmed Hosseini, Roger Hull, Alice K. Inoue-Nagata, Ramon Jordan, Hideki Kondo, Jan F. Kreuze, Mart Krupovic, Kenji Kubota, Jens H. Kuhn, Scott Leisner, Jean-Michel Lett, Chengyu Li, Fan Li, Jun Min Li, Paola M. López-Lambertini, Juan J. Lopez-Moya, Francois Maclot, Kristiina Mäkinen, Darren Martin, Sebastien Massart, W. Allen Miller, Musa Mohammadi, Dimitre Mollov, Emmanuelle Muller, Tatsuya Nagata, Jesús Navas-Castillo, Yutaro Neriya, Francisco M. Ochoa-Corona, Kazusato Ohshima, Vicente Pallás, Hanu Pappu, Karel Petrzik, Mikhail Pooggin, Maria Isabella Prigigallo, Pedro L. Ramos-González, Simone Ribeiro, Katja R. Richert-Pöggeler, Philippe Roumagnac, Avijit Roy, Sead Sabanadzovic, Dana Šafářová, Pasquale Saldarelli, Hélène Sanfaçon, Cecilia Sarmiento, Takahide Sasaya, Kay Scheets, Willem E.W. Schravesande, Susan Seal, Yoshifumi Shimomoto, Merike Sõmera, Livia Stavolone, Lucy R. Stewart, Pierre-Yves Teycheney, John E. Thomas, Jeremy R. Thompson, Antonio Tiberini, Yasuhiro Tomitaka, Ioannis Tzanetakis, Marie Umber, Cica Urbino, Harrold A. van den Burg, René A.A. Van der Vlugt, Arvind Varsani, Adriaan Verhage, Dan Villamor, Susanne von Bargen, Peter J. Walker, Thierry Wetzel, Anna E. Whitfield, Stephen J. Wylie, Caixia Yang, F. Murilo Zerbini, Song Zhang

    Journal of General Virology　106　(7)　002114　2025/07/25

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002114 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    In March 2025, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote, newly proposed taxa were added to those under the mandate of the Plant Viruses Subcommittee. In brief, 1 new order, 3 new families, 6 new genera, 2 new subgenera and 206 new species were created. Some taxa were reorganized. Genus Cytorhabdovirus in the family Rhabdoviridae was abolished and its taxa were redistributed into three new genera Alphacytorhabdovirus, Betacytorhabdovirus and Gammacytorhabdovirus. Genus Waikavirus in the family Secoviridae was reorganized into two subgenera (Actinidivirus and Ritunrivirus). One family and four previously unaffiliated genera were moved to the newly established order Tombendovirales. Twelve species not assigned to a genus were abolished. To comply with the ICTV mandate of a binomial format for virus species, eight species were renamed. Demarcation criteria in the absence of biological information were defined in the genus Ilarvirus (family Bromoviridae). This article presents the updated taxonomy put forth by the Plant Viruses Subcommittee and ratified by the ICTV.

  29. Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Animal DNA Viruses and Retroviruses Subcommittee, 2025 Peer-reviewed

    Arvind Varsani, Adly M.M. Abd-Alla, Niklas Arnberg, Kelly S. Bateman, Mária Benkő, Annie Bézier, Philippe Biagini, Jamie Bojko, Anamarija Butkovic, Marta Canuti, Vladimír Celer, Jean-Michel Drezen, Laszlo Egyed, Matthias G. Fischer, Sarah François, Benjamin Guinet, Balázs Harrach, Robert L. Harrison, Elisabeth A. Herniou, Michael Hess, Jia Hu, Johannes A. Jehle, Győző L. Kaján, Adrianna E. Kajon, Eugene V. Koonin, Simona Kraberger, Peter J. Krell, Mart Krupovic, Jens H. Kuhn, Chengfeng Lei, Matthieu Leobold, Fabrizio Maggi, Suresh K. Mittal, Hiroaki Okamoto, Tanja Opriessnig, Xiaowei Peng, Judit Pénzes, Iva I. Podgorski, Thomas S. Postler, Bergmann M. Ribeiro, Carmen San Martín, Maria Söderlund-Venermo, Xiulian Sun, András Surján, Zoltán L. Tarján, Julien Varaldi, Márton Z. Vidovszky, Göran Wadell, Hidemi Watanabe, Natalya Yutin, Monique M. van Oers

    Journal of General Virology　106　(7)　002113　2025/07/25

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002113 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    The International Committee on Taxonomy of Viruses (ICTV) holds a ratification vote annually after review of newly proposed taxa by ICTV Study Groups and members of the virology community. In March 2025, the vote outcome of the 11 proposals within the mandate of the Animal DNA Viruses and Retroviruses Subcommittee was made public. Here, we provide a summary of the newly accepted proposals. These include reorganization of taxa in the realm Varidnaviria, classification of the ‘polinton-like’ viruses into a new family (Phypoliviridae) within a new order Archintovirales; establishment of a new phylum (Commensaviricota) in the kingdom Shotokuvirae; the establishment of a new family called Filamentoviridae with two new genera and three new species; the addition of four new genera in the family Anelloviridae with 70 new species; and the addition of 85 new species in the families Adenoviridae (n=16), Baculoviridae (n=5), Circoviridae (n=5), Parvoviridae (n=55) and Polyomaviridae (n=4). Also, in the family Belpaoviridae, 11 species were renamed to comply with the binomial requirement for species names.

  30. Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Animal dsRNA and ssRNA(−) Viruses Subcommittee, 2025 Invited Peer-reviewed

    Holly R. Hughes, Matthew J. Ballinger, Yiming Bao, Nicolas Bejerman, Kim R. Blasdell, Thomas Briese, Julia Brignone, Jean Paul Carrera, Lander De Coninck, William Marciel de Souza, Humberto Debat, Ralf G. Dietzgen, Ralf Dürrwald, Mert Erdin, Anthony R. Fooks, Kristian M. Forbes, Juliana Freitas-Astúa, Jorge B. Garcia, Jemma L. Geoghegan, Rebecca M. Grimwood, Masayuki Horie, Timothy H. Hyndman, Reimar Johne, John D. Klena, Hideki Kondo, Eugene V. Koonin, Alexei Y. Kostygov, Mart Krupovic, Jens H. Kuhn, Michael Letko, Jun-Min Li, Yiyun Liu, Maria Laura Martin, Nathaniel Mull, Yael Nazar, Norbert Nowotny, Márcio Roberto Teixeira Nunes, Arnfinn Lodden Økland, Dennis Rubbenstroth, Brandy J. Russell, Eric Schott, Stephanie Seifert, Carina Sen, Elizabeth Shedroff, Tarja Sironen, Teemu Smura, Camila Prestes Dos Santos Tavares, Robert B. Tesh, Natasha L. Tilston, Noël Tordo, Nikos Vasilakis, Peter J. Walker, Fei Wang, Anna E. Whitfield, Shannon L.M. Whitmer, Yuri I. Wolf, Han Xia, Gong-Yin Ye, Zhuangxin Ye, Vyacheslav Yurchenko, Mingli Zhao

    Journal of General Virology　106　(7)　002112　2025/07/25

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002112 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    RNA viruses are ubiquitous in the environment and are important pathogens of humans, animals and plants. In 2024, the International Committee on Taxonomy of Viruses Animal dsRNA and ssRNA(−) Viruses Subcommittee submitted 18 taxonomic proposals for consideration. These proposals expanded the known virosphere by classifying 9 new genera and 88 species for newly detected virus genomes. Of note, newly established species expand the large family of Rhabdoviridae to 580 species. A new species in the family Arenaviridae includes a virus detected in Antarctic fish with a unique split nucleoprotein ORF. Additionally, four new species were established for historically isolated viruses with previously unsequenced genomes. Furthermore, three species were abolished due to incomplete genome sequence information, and one family was moved from being unassigned in the phylum Negarnaviricota into a subphylum and order. Herein, we summarize the 18 ratified taxonomic proposals and the general features of the current taxonomy, thereby supporting public and animal health responses.

  31. Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Bacterial Viruses Subcommittee, 2025 Invited Peer-reviewed

    Dann Turner, Evelien M. Adriaenssens, Rudolf I. Amann, Pavol Bardy, Nina Bartlau, Jakub Barylski, Stanisław Błażejak, Majid Bouzari, Ariane Briegel, Yves Briers, Daniel Carrillo, Xia Chen, Dennis Claessen, Ryan Cook, Marco A. Crisci, Arnaud Dechesne, Paulina Deptula, Bas E. Dutilh, Bert Ely, Lars Fieseler, Paul C.M. Fogg, Akihito Fukudome, Mohammed Saeed Ganjoor, Iwona Gientka, Karin Holmfeldt, Panos G. Kalatzis, Kathryn M. Kauffman, Annabel Kempff, Petar Knezevic, Eugene V. Koonin, Andrew M. Kropinski, Mart Krupovic, Ipek Kurtböke, Kai Lambon, Rob Lavigne, Susan M. Lehman, H.-T. Liu, Cedric Lood, Rudi Lurz, Sari Mäntynen, Cole B. Matrishin, Mathias Middelboe, Andrew D. Millard, Cristina Moraru, Dennis S. Nielsen, Franklin L. Nobrega, Takuro Nunoura, Hanna M. Oksanen, Véronique Ongenae, Boris Parra, Célia Pas, Joseph Pogliano, Minna M. Poranen, Siravudh Potipimpanon, Amy Prichard, Hannah V. Pye, Daniela Rothschild-Rodriguez, Daniel E. Rozen, Joanne M. Santini, Yuandong Sha, Dziyana Shymialevich, Barbara Sokołowska, Abbas Soleimani-Delfan, Paulina Średnicka, Paulo Tavares, Andrea Telatin, Igor Tolstoy, Shyun-ichi Urayama, Vera van Neer, Finn K. Vogensen, Qiannan Wen, Antje Wichels, Michał Wójcicki

    Journal of General Virology　106　(7)　002111　2025/07/25

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002111 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    This article summarises the activities of the International Committee on Taxonomy of Viruses Bacterial Viruses Subcommittee, detailing developments in the classification of bacterial viruses. We provide here an overview of all new, abolished, moved and renamed taxa proposed in 2024, approved by the Executive Committee, and ratified by membership vote in 2025. Through the collective efforts of 74 international contributors of taxonomy proposals in this round, 43 ratified proposals have led to the creation of one new phylum, one class, four orders, 33 families, 14 subfamilies, 194 genera and 995 species. These proposals mark significant progress in refining the taxonomy of bacterial viruses. Key updates include the creation of new orders and families that include existing taxa to better reflect genomic and evolutionary relationships. As sequencing and bioinformatics approaches continue to advance, further expansion and refinements in viral taxonomy can be anticipated in the coming years.

  32. Virus taxonomy proposal summaries: a searchable and citable resource to disseminate virus taxonomy advances Invited Peer-reviewed

    Richard Mayne, Peter Simmonds, Donald B. Smith, Evelien M. Adriaenssens, Elliot J. Lefkowitz, Hanna M. Oksanen, Francisco Murilo Zerbini, Poliane Alfenas-Zerbini, Frank O Aylward, Juliana Freitas-Astúa, R. Curtis Hendrickson, Holly R. Hughes, Mart Krupovic, Jens H. Kuhn, Małgorzata Łobocka, Arcady R. Mushegian, Judit Penzes, Alejandro Reyes Muñoz, David L. Robertson, Simon Roux, Luisa Rubino, Sead Sabanadzovic, Nobuhiro Suzuki, Dann Turner, Koenraad Van Doorslaer, Arvind Varsani

    Journal of General Virology　106　(7)　002079　2025/07/25

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.002079 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    Taxonomic classification of cellular organisms requires the publication of descriptions and proposed names of species and the deposition of specimens. Virus taxonomy is developed through a different system of annual submission of formal taxonomy proposals (TPs) that can be submitted by anyone but are typically prepared by a study group appointed by the International Committee on Taxonomy of Viruses (ICTV) and consisting of experts on a particular group of viruses. These are initially evaluated by an expert subcommittee and by the executive committee (EC) of the ICTV. EC-approved TPs are then submitted for evaluation and a ratification vote by the wider ICTV membership. Following ratification, the new taxonomy is annually updated in the Master Species List, associated databases and bioinformatic resources. The process is consistent, creates traceability in assignments and supports a fully evaluated, hierarchical classification and nomenclature of all taxonomic ranks from species to realms. The structure also facilitates large-scale and coordinated changes to virus taxonomy, such as the recent introduction of a binomial species nomenclature. TPs are available on the ICTV website after ratification, but they are not indexed in bibliographic databases and are not easily cited. Authors of TPs do not receive citation credit for adopted proposals, and their voluntary contributions are largely invisible in the published literature. For greater visibility of TPs and their authors, the ICTV will commence the annual publication of summaries of all TPs from each ICTV subcommittee. These summaries will provide a searchable compendium of all annual taxonomy changes and additions as well as direct links to the Master Species List and other ICTV bioinformatic resources. Their publication will provide due credit and citations for their authors, form the basis for disseminating taxonomy decisions and promote greater visibility and accessibility to taxonomy changes for the virology community.

  33. Virus species names have been standardized; virus names remain unchanged Invited Peer-reviewed

    F. Murilo Zerbini, Peter Simmonds, Evelien M. Adriaenssens, Elliot J. Lefkowitz, Hanna M. Oksanen, Poliane Alfenas-Zerbini, Frank O. Aylward, Juliana Freitas-Astúa, Holly R. Hughes, Małgorzata Łobocka, Mart Krupovic, Jens H. Kuhn, Arcady Mushegian, Judit J. Penzes, Alejandro Reyes, David L. Robertson, Simon Roux, Luisa Rubino, Sead Sabanadzovic, Donald B. Smith, Nobuhiro Suzuki, Dann Turner, Koenraad Van Doorslaer, Arvind Varsani

    mSphere　10　(5)　e00020-25　2025/05/27

    Publisher: American Society for Microbiology

    DOI： 10.1128/msphere.00020-25 　

    eISSN： 2379-5042

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    ABSTRACT Virus taxonomy, comprising classification and nomenclature, is regulated by the International Committee on Taxonomy of Viruses (ICTV). Taxon names are standardized to facilitate recognition and communication, with defined suffixes for each rank (e.g., the names of orders, families, and genera end in -virales , - viridae, and -virus , respectively). However, until recently, a standard format for species names was lacking. In 2021, following extensive discussion and community consultation, the ICTV decided to adopt a standardized binomial (Linnaean) format for virus species names, consisting of the genus name followed by a “freeform” species epithet. Previously assigned virus species names that were non-compliant with the binomial format have been fully updated. In contrast to taxon names regulated by the ICTV, the names of viruses, or “common” names, such as yellow fever virus or human immunodeficiency virus, are not under the remit of the ICTV and have not been changed.

  34. A capsidless (+)RNA yadokarivirus hosted by a dsRNA virus is infectious as particles, cDNA, and dsRNA. International-journal

    Muhammad Fadli, Sakae Hisano, Guy Novoa, José R Castón, Hideki Kondo, Nobuhiro Suzuki

    Journal of virology　99　(3)　e0216624　2025/03/18

    DOI： 10.1128/jvi.02166-24 　

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    Capsidless yadokariviruses (members of the order Yadokarivirales) with (+)RNA genomes divert the capsid of their partner icosahedral double-stranded RNA (dsRNA) viruses in different families of the order Ghabrivirales into the replication site. A yadokarivirus, AfSV2, has been reported from a German strain of the ascomycete fungus Aspergillus foetidus coinfected by two dsRNA viruses, a victorivirus (AfSV1, family Pseudototiviridae) and an alternavirus (AfFV, family Alternaviridae). Here, we identified AfSV1 as the partner of AfSV2 in a Japanese A. foetidus strain after showing the infectiousness of AfSV2 in three forms: virus particles (heterocapsid), transforming full-length complementary DNA (cDNA), and purified replicated form (RF) dsRNA that is believed to be inactive as a translational template. Virion transfection of virus-free A. foetidus protoplasts resulted in the generation of two strains infected either by AfSV1 alone or by both AfSV1 and AfSV2. Transformants with AfSV2 full-length cDNA launched AfSV2 infection only in the presence of AfSV1, but not those with AfSV2 RNA-directed RNA polymerase mutant cDNA. The purified fractions containing AfSV2 RF dsRNA also launched infection when transfected into protoplasts infected by AfSV1. Treatment with dsRNA-specific RNase III, but not with proteinase K, S1 nuclease, or DNase I, abolished the infectivity of AfSV2 RF dsRNA. Furthermore, we confirmed the infectiousness of gel-purified AfSV2 RF dsRNA in the presence of AfSV1. Taken together, our results show the unique infectious entity of AfSV2 and the expansion of yadokarivirus partners in the family Pseudototiviridae and provide interesting evolutionary insights.IMPORTANCEThe viral phylum Pisuviricota accommodates members with both double-stranded RNA (dsRNA) and (+)RNA genomes. Some members of the second group display peculiar virus lifestyles. These include (+)RNA yadkariviruses, which are capsidless and highjack the capsid of their partner dsRNA viruses in the order Ghabrivirales of a different phylum Duplornaviricota. We identified the partner dsRNA virus (AfSV1, a victorivirus) of a yadokarivirus (AfSV2) from the ascomycete Aspergillus foetidus. AfSV2 is infectious in the presence of AfSV1 in three forms: purified particles, transforming full-length complementary DNA, and, surprisingly, the purified replicative form dsRNA. These combined results expand yadokarivirus partner viruses to the family Pseudototiviridae and provide evidence for AfSV2 as a unique infectious entity as well as interesting evolutionary insights.

  35. Evidence for the replication of a plant rhabdovirus in its arthropod mite vector. International-journal

    Hideki Kondo, Miki Fujita, Paul Telengech, Kazuyuki Maruyam, Kiwamu Hyodo, Aline Daniele Tassi, Ronald Ochoa, Ida Bagus Andika, Nobuhiro Suzuki

    Virus research　351　199522-199522　2025/01/04

    DOI： 10.1016/j.virusres.2024.199522 　

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    Transmission of plant viruses that replicate in the insect vector is known as persistent-propagative manner. However, it remains unclear whether such virus-vector relationships also occur between plant viruses and other biological vectors such as arthropod mites. In this study, we investigated the possible replication of orchid fleck virus (OFV), a segmented plant rhabdovirus, within its mite vector (Brevipalpus californicus s.l.) using quantitative RT-qPCR, western blotting and next-generation sequencing. Time-course RT-qPCR and western blot analyses showed an increasing OFV accumulation pattern in mites after virus acquisition. Since OFV genome expression requires the transcription of polyadenylated mRNAs, polyadenylated RNA fractions extracted from the viruliferous mite samples and OFV-infected plant leaves were used for RNA-seq analysis. In the mite and plant datasets, a large number of sequence reads were aligned to genomic regions of OFV RNA1 and RNA2 corresponding to transcribed viral gene mRNAs. This includes the short polyadenylated transcripts originating from the leader and trailer regions at the ends of the viral genome, which are believed to play a crucial role in viral transcription/replication. In contrast, a low number of reads were mapped to the non-transcribed regions (gene junctions). These results strongly suggested that OFV gene expression occurs both in mites and plants. Additionally, deep sequencing revealed the accumulation of OFV-derived small RNAs in mites, although their size profiles differ from those found in plants. Taken together, our results indicated that OFV replicates within a mite vector and is targeted by the RNA-silencing mechanism.

  36. Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2024) Peer-reviewed

    Peter Simmonds, Evelien M. Adriaenssens, Elliot J. Lefkowitz, Hanna M. Oksanen, Stuart G. Siddell, Francisco Murilo Zerbini, Poliane Alfenas-Zerbini, Frank O. Aylward, Donald M. Dempsey, Bas E. Dutilh, Juliana Freitas-Astúa, María Laura García, R. Curtis Hendrickson, Holly R. Hughes, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Arcady R. Mushegian, Judit Penzes, Alejandro Reyes Muñoz, David L. Robertson, Simon Roux, Luisa Rubino, Sead Sabanadzovic, Donald B. Smith, Nobuhiro Suzuki, Dann Turner, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani

    Archives of Virology　169　(11)　2024/11/03

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-024-06143-y 　

    ISSN： 0304-8608

    eISSN： 1432-8798

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    Abstract This article reports changes to virus taxonomy and taxon nomenclature that were approved and ratified by the International Committee on Taxonomy of Viruses (ICTV) in April 2024. The entire ICTV membership was invited to vote on 203 taxonomic proposals that had been approved by the ICTV Executive Committee (EC) in July 2023 at the 55th EC meeting in Jena, Germany, or in the second EC vote in November 2023. All proposals were ratified by online vote. Taxonomic additions include one new phylum (Ambiviricota), one new class, nine new orders, three new suborders, 51 new families, 18 new subfamilies, 820 new genera, and 3547 new species (excluding taxa that have been abolished). Proposals to complete the process of species name replacement to the binomial (genus + species epithet) format were ratified. Currently, a total of 14,690 virus species have been established.

  37. Virus Research: 40 years and still going strong Invited Peer-reviewed

    Ben Berkhout, Esteban Domingo, Nobuhiro Suzuki

    Virus Research　350　199493-199493　2024/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.virusres.2024.199493 　

    ISSN： 0168-1702

  38. Ancient environmental microbiomes and the cryosphere Peer-reviewed

    Alexander D. Williams, Vivian W. Leung, Julian W. Tang, Nishimura Hidekazu, Nobuhiro Suzuki, Andrew C. Clarke, David A. Pearce, Tommy Tsan-Yuk Lam

    Trends in Microbiology　2024/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.tim.2024.09.010 　

    ISSN： 0966-842X

  39. New lineages of RNA viruses from clinical isolates of Rhizopus microsporus revealed by fragmented and primer-ligated dsRNA sequencing (FLDS) analysis. International-journal Peer-reviewed

    Wasiatus Sa'diyah, Yan-Jie Zhao, Yuto Chiba, Hideki Kondo, Nobuhiro Suzuki, Sayaka Ban, Takashi Yaguchi, Syun-Ichi Urayama, Daisuke Hagiwara

    mSphere　9　e0034524　2024/07/29

    DOI： 10.1128/msphere.00345-24 　

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    Rhizopus microsporus is a species in the order Mucorales that is known to cause mucormycosis, but it is poorly understood as a host of viruses. Here, we examined 25 clinical strains of R. microsporus for viral infection with a conventional double-stranded RNA (dsRNA) assay using agarose gel electrophoresis (AGE) and the recently established fragmented and primer-ligated dsRNA sequencing (FLDS) protocol. By AGE, five virus-infected strains were detected. Then, full-length genomic sequences of 12 novel RNA viruses were revealed by FLDS, which were related to the families Mitoviridae, Narnaviridae, and Endornaviridae, ill-defined groups of single-stranded RNA (ssRNA) viruses with similarity to the established families Virgaviridae and Phasmaviridae, and the proposed family "Ambiguiviridae." All the characterized viruses, except a potential phasmavirid with a negative-sense RNA genome, had positive-sense RNA genomes. One virus belonged to a previously established species within the family Mitoviridae, whereas the other 11 viruses represented new species or even new genera. These results show that the fungal pathogen R. microsporus harbors diverse RNA viruses and extend our understanding of the diversity of RNA viruses in the fungal order Mucorales, division Mucoromycota. Identifying RNA viruses from clinical isolates of R. microsporus may expand the repertoire of natural therapeutic agents for mucormycosis in the future.IMPORTANCEThe diversity of mycoviruses in fungal hosts in the division Mucoromycota has been underestimated, mainly within the species Rhizopus microsporus. Only five positive-sense RNA genomes had previously been discovered in this species. Because current sequencing methods poorly complete the termini of genomes, we used fragmented and primer-ligated double-stranded RNA sequencing to acquire the full-length genomes. Eleven novel mycoviruses were detected in this study, including the first negative-sense RNA genome reported in R. microsporus. Our findings extend the understanding of the viral diversity in clinical strains of Mucoromycota, may provide insights into the pathogenesis and ecology of this fungus, and may offer therapeutic options.

  40. Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses. International-journal Peer-reviewed

    Michael Louie R Urzo, Timothy D Guinto, Ana Eusebio-Cope, Bernard O Budot, Mary Jeanie T Yanoria, Gilda B Jonson, Masao Arakawa, Hideki Kondo, Nobuhiro Suzuki

    Viruses　16　(7)　2024/07/17

    DOI： 10.3390/v16071152 　

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    Rice sheath blight, caused by the soil-borne fungus Rhizoctonia solani (teleomorph: Thanatephorus cucumeris, Basidiomycota), is one of the most devastating phytopathogenic fungal diseases and causes yield loss. Here, we report on a very high prevalence (100%) of potential virus-associated double-stranded RNA (dsRNA) elements for a collection of 39 fungal strains of R. solani from the rice sheath blight samples from at least four major rice-growing areas in the Philippines and a reference isolate from the International Rice Research Institute, showing different colony phenotypes. Their dsRNA profiles suggested the presence of multiple viral infections among these Philippine R. solani populations. Using next-generation sequencing, the viral sequences of the three representative R. solani strains (Ilo-Rs-6, Tar-Rs-3, and Tar-Rs-5) from different rice-growing areas revealed the presence of at least 36 viruses or virus-like agents, with the Tar-Rs-3 strain harboring the largest number of viruses (at least 20 in total). These mycoviruses or their candidates are believed to have single-stranded RNA or dsRNA genomes and they belong to or are associated with the orders Martellivirales, Hepelivirales, Durnavirales, Cryppavirales, Ourlivirales, and Ghabrivirales based on their coding-complete RNA-dependent RNA polymerase sequences. The complete genome sequences of two novel RNA viruses belonging to the proposed family Phlegiviridae and family Mitoviridae were determined.

  41. Exploration of the yadokari/yadonushi nature of YkV3 and RnMBV3 in the original host and a model filamentous fungus. International-journal Peer-reviewed

    Yukiyo Sato, Sakae Hisano, Nobuhiro Suzuki

    Virus research　334　199155-199155　2023/06/27

    DOI： 10.1016/j.virusres.2023.199155 　

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    The yadokari/yadonushi nature is a recently discovered virus lifestyle; "yadokari" refers to the ability of capsidless positive-sense (+) RNA viruses (yadokariviruses) to utilize the capsids of phylogenetically distant double-stranded RNA (dsRNA) viruses possibly as the replication site, while "yadonushi" refers to the ability of dsRNA viruses to provide capsids to yadokariviruses. This virus-virus interaction, however, has been only studied with limited pathosystems. Here, we established a new study model with a capsidless (+)RNA yadokarivirus YkV3 (family Yadokariviridae) and its capsid donor RnMBV3 (family Megabirnaviridae) in the original host fungus Rosellinia necatrix and a model filamentous fungal host Cryphonectria parasitica. YkV3 has a simple genome structure with one open reading frame of 4305 nucleotides encoding a single polyprotein with an RNA-dependent RNA polymerase and a 2A-like self-cleavage peptide domain. Reverse genetics of YkV3 in R. necatrix showed that YkV3 tolerates a nucleotide substitution in the extreme 5'-terminus. The insertion of two termination codons immediately downstream of the 2A-like cleavage site abolished YkV3 viability, suggesting the importance of the C-terminal portion of the polyprotein of unknown function. Transfection of RnMBV3 and YkV3 into an RNA silencing-deficient mutant Δdcl2 of C. parasitica showed the replication competency of both viruses. Comparison between the wild-type and Δdcl2 strains of C. parasitica in virus accumulation suggested that RnMBV3 and YkV3 are susceptible to RNA silencing in C. parasitica. Taken together, we have established a platform to further explore the yadokari/yadonushi nature using genetically manipulable host fungal and virus strains.

  42. Continued mycovirus discovery expanding our understanding of virus lifestyles, symptom expression, and host defense. International-journal Peer-reviewed

    Yukiyo Sato, Nobuhiro Suzuki

    Current opinion in microbiology　75　102337-102337　2023/06/19

    DOI： 10.1016/j.mib.2023.102337 　

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    High-throughput sequencing technologies have greatly expanded the RNA virome in general and have led to an exponential increase in new fungal viruses, also known as mycoviruses. Mycoviruses are omnipresent in fungi and usually induce symptomless infections. Some mycoviruses infecting fungi pathogenic to plants, insects, and mammals are known to modify host virulence positively and negatively and attract particular interests. In addition, fungal viruses continue to provide intriguing research materials and themes that lead to discoveries of peculiar viruses as infectious entities and insights into virus evolution and diversity. In this review, we outline the diversity and neolifestyle of recently discovered fungal RNA viruses, and phenotypic alterations induced by them. Furthermore, we discuss recent advances in research regarding the fungal antiviral defense and viral counterdefense, which are closely associated with host phenotype alterations. We hope that this article will enhance understanding of the interesting and growing fungal virology field.

  43. Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2023)

    Francisco Murilo Zerbini, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas‑Zerbini, Donald M. Dempsey, Bas E. Dutilh, María Laura García, R. Curtis Hendrickson, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne‑Mieke Vandamme, Arvind Varsani

    Archives of Virology　168　(7)　175　2023/06/10

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-023-05797-4 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  44. ICTV Virus Taxonomy Profile: Hypoviridae 2023 Peer-reviewed

    Sotaro Chiba, Leonardo Velasco, María A. Ayllón, Nobuhiro Suzuki, Shin-Yi Lee-Marzano, Lying Sun, Sead Sabanadzovic, Massimo Turina

    Journal of General Virology　104　(5)　001848　2023/05/31

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.001848 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    Hypoviridae is a family of capsidless viruses with positive-sense RNA genomes of 7.3–18.3 kb that possess either a single large open reading frame (ORF) or two ORFs. The ORFs appear to be translated from genomic RNA by non-canonical mechanisms, i.e. internal ribosome entry site- and stop/restart translation. This family includes the genera Alphahypovirus, Betahypovirus, Gammahypovirus, Deltahypovirus, Epsilonhypovirus, Zetahypovirus, Thetahypovirus and Etahypovirus. Hypovirids have been detected in ascomycetous and basidiomycetous filamentous fungi and are considered to replicate in host, Golgi apparatus-derived, lipid vesicles that contain virus dsRNA as the replicative form. Some hypovirids induce hypovirulence to host fungi, while others do not. This is a summary of the ICTV report on the family Hypoviridae, which is available at www.ictv.global/report/hypoviridae.

  45. Sequence and phylogenetic analysis of a novel alphaendornavirus, the first virus described from the oomycete plant pathogen Phytophthora heveae Peer-reviewed

    Milica Raco, Thomas Jung, Marilia Horta Jung, Nguyen Minh Chi, Leticia Botella, Nobuhiro Suzuki

    Archives of Virology　168　(6)　2023/05/11

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-023-05786-7 　

    ISSN： 0304-8608

    eISSN： 1432-8798

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    Abstract Here, we report the discovery and complete genome sequence of a novel virus, designated as “Phytophthora heveae alphaendornavirus 1” (PhAEV1), from a single isolate of the plant pathogenic oomycete Phytophthora heveae (kingdom Stramenipila) isolated from a tropical evergreen lowland rainforest in northern Vietnam. PhAEV1 was detected by both cellulose affinity chromatography of dsRNA and high-throughput sequencing of total RNA, and its presence and sequence were confirmed by RT-PCR and Sanger sequencing. The PhAEV1 genome, 12,820 nucleotides (nt) in length, was predicted to encode a single large polyprotein with the catalytic core domain of viral (superfamily 1) RNA helicase (HEL, amino acid [aa] positions 1,287-1,531), glycosyltransferase (GT, aa positions ca. 2,800-3,125), and RNA-directed RNA polymerase (RdRp, aa positions 3,875-4,112). PhAEV1 is the most similar to Phytophthora cactorum alphaendornavirus 3, sharing 39.4% and 39.1% nt and aa sequence identity, respectively. In addition to the first 5′-terminal AUG codon, three additional in-frame methionine codons were found in close proximity (nt 14-16, 96-98, and 176-178), suggesting potential additional translation initiation sites. Conserved RdRp motifs (A-E) similar to those detected in related endornaviruses were identified in PhAEV1, as well as in several previously described alphaendornaviruses from other Phytophthora species in which these motifs had not been identified previously. Phylogenetic analysis showed that PhAEV1 clusters with members of the genus Alphaendornavirus in the family Endornaviridae and is basal to two other alphaendornaviruses described from another oomycete, Phytophthora cactorum. PhAEV1 is the first virus reported in P. heveae.

  46. Virus taxonomy and the role of the International Committee on Taxonomy of Viruses (ICTV) Peer-reviewed

    Stuart G. Siddell, Donald B. Smith, Evelien Adriaenssens, Poliane Alfenas-Zerbini, Bas E. Dutilh, Maria Laura Garcia, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Elliot J. Lefkowitz, Małgorzata Łobocka, Arcady R. Mushegian, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, F. Murilo Zerbini

    Journal of General Virology　104　(5)　2023/05/04

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.001840 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    The taxonomy of viruses is developed and overseen by the International Committee on Taxonomy of Viruses (ICTV), which scrutinizes, approves and ratifies taxonomic proposals, and maintains a list of virus taxa with approved names (https://ictv.global). The ICTV has approximately 180 members who vote by simple majority. Taxon-specific Study Groups established by the ICTV have a combined membership of over 600 scientists from the wider virology community; they provide comprehensive expertise across the range of known viruses and are major contributors to the creation and evaluation of taxonomic proposals. Proposals can be submitted by anyone and will be considered by the ICTV irrespective of Study Group support. Thus, virus taxonomy is developed from within the virology community and realized by a democratic decision-making process. The ICTV upholds the distinction between a virus or replicating genetic element as a physical entity and the taxon category to which it is assigned. This is reflected by the nomenclature of the virus species taxon, which is now mandated by the ICTV to be in a binomial format (genus + species epithet) and is typographically distinct from the names of viruses. Classification of viruses below the rank of species (such as, genotypes or strains) is not within the remit of the ICTV. This article, authored by the ICTV Executive Committee, explains the principles of virus taxonomy and the organization, function, processes and resources of the ICTV, with the aim of encouraging greater understanding and interaction among the wider virology community.

  47. Structural features of T-DNA that induce transcriptional gene silencing during agroinfiltration Peer-reviewed

    Emi Iida, Kazunori Kuriyama, Midori Tabara, Atsushi Takeda, Nobuhiro Suzuki, Hiromitsu Moriyama, Toshiyuki Fukuhara

    2023/04/14

    Publisher: Research Square Platform {LLC}

    DOI： 10.21203/rs.3.rs-2796710/v1 　

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    <jats:title>Abstract</jats:title> <jats:p><jats:italic>Agrobacterium tumefaciens</jats:italic> (<jats:italic>Rhizobium radiobacter</jats:italic>) is used for the transient expression of foreign genes by the agroinfiltration method, but the introduction of foreign genes often induces transcriptional and/or post-transcriptional gene silencing (TGS and/or PTGS). In this study, we characterized the structural features of T-DNA that induce TGS during agroinfiltration. When <jats:italic>A. tumefaciens</jats:italic>cells harboring an empty T-DNA plasmid containing the cauliflower mosaic virus (CaMV) 35S promoter were infiltrated into the leaves<jats:italic> </jats:italic>of <jats:italic>Nicotiana benthamiana</jats:italic> line 16c with a GFP gene over-expressed under the control of the same promoter, no small interfering RNAs (siRNAs) were derived from the GFP sequence. However, siRNAs derived from the CaMV 35S promoter were detected, indicating that TGS against the GFP gene was induced. When the GFP gene was inserted into the T-DNA plasmid, PTGS against the GFP gene was induced whereas TGS against the CaMV 35S promoter was suppressed. In other words, depending on the combination of promoter and coding sequences on T-DNA and the host nuclear genome, either or both TGS and/or PTGS could be induced by agroinfiltration. We also showed the importance of terminator sequences in T-DNA for gene silencing and the possible involvement of three siRNA-producing Dicers in the induction of TGS. These results are valuable for controlling gene expression by agroinfiltration.</jats:p>

  48. Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan Peer-reviewed

    Hideki Kondo, Hitomi Sugahara, Miki Fujita, Kiwamu Hyodo, Ida Bagus Andika, Hiroshi Hisano, Nobuhiro Suzuki

    Pathogens　12　(3)　358-358　2023/02/21

    Publisher: MDPI AG

    DOI： 10.3390/pathogens12030358 　

    eISSN： 2076-0817

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    Many aphid-borne viruses are important pathogens that affect wheat crops worldwide. An aphid-transmitted closterovirus named wheat yellow leaf virus (WYLV) was found to have infected wheat plants in Japan in the 1970s; however, since then, its viral genome sequence and occurrence in the field have not been investigated. We observed yellowing leaves in the 2018/2019 winter wheat-growing season in an experimental field in Japan where WYLV was detected five decades ago. A virome analysis of those yellow leaf samples lead to the discovery of a closterovirus together with a luteovirus (barley yellow dwarf virus PAV variant IIIa). The complete genomic sequence of this closterovirus, named wheat closterovirus 1 isolate WL19a (WhCV1-WL19a), consisted of 15,452 nucleotides harboring nine open reading frames. Additionally, we identified another WhCV1 isolate, WL20, in a wheat sample from the winter wheat-growing season of 2019/2020. A transmission test indicated that WhCV1-WL20 was able to form typical filamentous particles and transmissible by oat bird-cherry aphid (Rhopalosiphum pad). Sequence and phylogenetic analyses showed that WhCV1 was distantly related to members of the genus Closterovirus (family Closteroviridae), suggesting that the virus represents a novel species in the genus. Furthermore, the characterization of WhCV1-WL19a-derived small RNAs using high-throughput sequencing revealed highly abundant 22-nt-class small RNAs potentially derived from the 3′-terminal end of the WhCV1 negative-strand genomic RNA, indicating that this terminal end of the WhCV1 genome is likely particularly targeted for the synthesis of viral small RNAs in wheat plants. Our results provide further knowledge on closterovirus diversity and pathogenicity and suggest that the impact of WhCV1 on wheat production warrants further investigations.

  49. Four principles to establish a universal virus taxonomy Peer-reviewed

    Peter Simmonds, Evelien M. Adriaenssens, F. Murilo Zerbini, Nicola G. A. Abrescia, Pakorn Aiewsakun, Poliane Alfenas-Zerbini, Yiming Bao, Jakub Barylski, Christian Drosten, Siobain Duffy, W. Paul Duprex, Bas E. Dutilh, Santiago F. Elena, Maria Laura García, Sandra Junglen, Aris Katzourakis, Eugene V. Koonin, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Elliot J. Lefkowitz, Małgorzata Łobocka, Cédric Lood, Jennifer Mahony, Jan P. Meier-Kolthoff, Arcady R. Mushegian, Hanna M. Oksanen, Minna M. Poranen, Alejandro Reyes-Muñoz, David L. Robertson, Simon Roux, Luisa Rubino, Sead Sabanadzovic, Stuart Siddell, Tim Skern, Donald B. Smith, Matthew B. Sullivan, Nobuhiro Suzuki, Dann Turner, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, Nikos Vasilakis

    PLOS Biology　21　(2)　e3001922-e3001922　2023/02/13

    Publisher: Public Library of Science (PLoS)

    DOI： 10.1371/journal.pbio.3001922 　

    eISSN： 1545-7885

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    A universal taxonomy of viruses is essential for a comprehensive view of the virus world and for communicating the complicated evolutionary relationships among viruses. However, there are major differences in the conceptualisation and approaches to virus classification and nomenclature among virologists, clinicians, agronomists, and other interested parties. Here, we provide recommendations to guide the construction of a coherent and comprehensive virus taxonomy, based on expert scientific consensus. Firstly, assignments of viruses should be congruent with the best attainable reconstruction of their evolutionary histories, i.e., taxa should be monophyletic. This fundamental principle for classification of viruses is currently included in the International Committee on Taxonomy of Viruses (ICTV) code only for the rank of species. Secondly, phenotypic and ecological properties of viruses may inform, but not override, evolutionary relatedness in the placement of ranks. Thirdly, alternative classifications that consider phenotypic attributes, such as being vector-borne (e.g., “arboviruses”), infecting a certain type of host (e.g., “mycoviruses,” “bacteriophages”) or displaying specific pathogenicity (e.g., “human immunodeficiency viruses”), may serve important clinical and regulatory purposes but often create polyphyletic categories that do not reflect evolutionary relationships. Nevertheless, such classifications ought to be maintained if they serve the needs of specific communities or play a practical clinical or regulatory role. However, they should not be considered or called taxonomies. Finally, while an evolution-based framework enables viruses discovered by metagenomics to be incorporated into the ICTV taxonomy, there are essential requirements for quality control of the sequence data used for these assignments. Combined, these four principles will enable future development and expansion of virus taxonomy as the true evolutionary diversity of viruses becomes apparent.

  50. Capsid structure of a fungal dsRNA megabirnavirus reveals its previously unidentified surface architecture Peer-reviewed

    Wang, H, Salaipeth, L, Miyazaki, N, Suzuki, N, Okamoto, K

    PLoS Pathogens　19　e1011162-e1011162　2023/02

    DOI： 10.1371/journal.ppat.1011162 　

  51. ICTV Virus Taxonomy Profile: Hadakaviridae. Invited Peer-reviewed

    Sato, Y, Turina, M, Chiba, S, Okada, R. A, Bhatti, M. F, Kotta-Loizou, I, Coutts, R. H. A, Kondo, H, Sabanadzovic, S, Suzuki, N, ICTV Repor, Consortium

    Journal of General Virology　104　001820　2023/02

  52. ICTV Virus Taxonomy Profile: Yadokariviridae 2023 Peer-reviewed

    Yukiyo Sato, Subha Das, Leonardo Velasco, Massimo Turina, Hideki Osaki, Ioly Kotta-Loizou, Robert H. A. Coutts, Hideki Kondo, Sead Sabanadzovic, Nobuhiro Suzuki

    Journal of General Virology　104　(1)　001826　2023/01/27

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.001826 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    The family Yadokariviridae, with the genera Alphayadokarivirus and Betayadokarivirus, includes capsidless non-segmented positive-sense (+) RNA viruses that hijack capsids from phylogenetically distant double-stranded RNA viruses. Yadokarivirids likely replicate inside the hijacked heterocapsids using their own RNA-directed RNA polymerase, mimicking dsRNA viruses despite their phylogenetic placement in a (+) RNA virus lineage. Yadokarivirids can have negative or positive impacts on their host fungi, through interactions with the capsid donor dsRNA viruses. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) report on the family Yadokariviridae, which is available at ictv.global/report/yadokariviridae.

  53. The simplest RNA replicons, viroids: A tribute to Ricardo Flores Invited

    Vicente Pallas, Francesco Di Serio, Nobuhiro Suzuki

    Virus Research　323　198996-198996　2023/01

    Publisher: Elsevier BV

    DOI： 10.1016/j.virusres.2022.198996 　

    ISSN： 0168-1702

  54. ICTV Virus Taxonomy Profile: Spinareoviridae 2022 Peer-reviewed

    Jelle Matthijnssens, Houssam Attoui, Krisztián Bányai, Corina P. D. Brussaard, Pranav Danthi, Mariana del Vas, Terence S. Dermody, Roy Duncan, Qín Fāng, Reimar Johne, Peter P. C. Mertens, Fauziah Mohd Jaafar, John T. Patton, Takahide Sasaya, Nobuhiro Suzuki, Taiyun Wei

    Journal of General Virology　103　(11)　2022/11/01

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.001781 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    Spinareoviridae is a large family of icosahedral viruses that are usually regarded as non-enveloped with segmented (9–12 linear segments) dsRNA genomes of 23–29 kbp. Spinareovirids have a broad host range, infecting animals, fungi and plants. Some have important pathogenic potential for humans (e.g. Colorado tick fever virus), livestock (e.g. avian orthoreoviruses), fish (e.g. aquareoviruses) and plants (e.g. rice ragged stunt virus and rice black streaked dwarf virus). This is a summary of the ICTV Report on the family Spinareoviridae, which is available at ictv.global/report/spinareoviridae.

  55. Recent changes to virus taxonomy ratified by the International Committee on Taxonomy of Viruses (2022) Peer-reviewed

    Peter J. Walker, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Donald M. Dempsey, Bas E. Dutilh, María Laura García, R. Curtis Hendrickson, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Hanna M. Oksanen, Richard J. Orton, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani, Francisco Murilo Zerbini

    Archives of Virology　167　(11)　2429-2440　2022/11

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-022-05516-5 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  56. Identification of novel totiviruses from the ascomycetous fungus Geotrichum candidum. International-journal

    Haris Ahmed Khan, Hideki Kondo, Sabitree Shahi, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of virology　2022/10/22

    DOI： 10.1007/s00705-022-05611-7 　

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    Mycoviruses are widely distributed across the kingdom Fungi, including ascomycetous yeast strains of the class Saccharomycetes. Geotrichum candidum is an important fungal pathogen belonging to Saccharomycetes and has a diverse host range. Here, we report the characterization of four new classical totiviruses from two distinct Geotrichum candidum strains from Pakistan. The four identified viruses were tentatively named "Geotrichum candidum totivirus 1, 2, 3a, and 3b" (GcTV1-3b). The complete dsRNA genomes of the identified totiviruses are 4621, 4592, 4576, and 4576 bp in length, respectively. All totivirus genomes have two open reading frames, encoding a capsid protein (CP) and an RNA-dependent RNA polymerase (RdRP), respectively. The downstream RdRP domain is assumed to be expressed as a CP-RdRP fusion product via -1 frameshifting mediated by a heptameric slippery site. Sequence comparisons and phylogenetic analysis showed that each of the discovered viruses belongs to a new species of the genus Totivirus in the family Totiviridae, with GcTV1 and GcTV3 (a and b strains) clustering in one subgroup and GcTV2 in another subgroup.

  57. ICTV Virus Taxonomy Profile: Sedoreoviridae 2022 Peer-reviewed

    Jelle Matthijnssens, Houssam Attoui, Krisztián Bányai, Corina P. D. Brussaard, Pranav Danthi, Mariana del Vas, Terence S. Dermody, Roy Duncan, Qín Fāng (方勤), Reimar Johne, Peter P. C. Mertens, Fauziah Mohd Jaafar, John T. Patton, Takahide Sasaya (笹谷孝英), Nobuhiro Suzuki (鈴木信弘), Taiyun Wei (魏太云)

    Journal of General Virology　103　(10)　2022/10/10

    Publisher: Microbiology Society

    DOI： 10.1099/jgv.0.001782 　

    ISSN： 0022-1317

    eISSN： 1465-2099

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    Sedoreoviridae is a large family of icosahedral viruses that are usually regarded as non-enveloped with segmented (10–12 linear segments) dsRNA genomes of 18–26 kbp. Sedoreovirids have a broad host range, infecting mammals, birds, crustaceans, arthropods, algae and plants. Some of them have important pathogenic potential for humans (e.g. rotavirus A), livestock (e.g. bluetongue virus) and plants (e.g. rice dwarf virus). This is a summary of the ICTV Report on the family Sedoreoviridae, which is available at ictv.global/report/sedoreoviridae.

  58. A Transfectable Fusagravirus from a Japanese Strain of Cryphonectria carpinicola with Spherical Particles. International-journal Peer-reviewed

    Subha Das, Sakae Hisano, Ana Eusebio-Cope, Hideki Kondo, Nobuhiro Suzuki

    Viruses　14　(8)　2022/08/04

    DOI： 10.3390/v14081722 　

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    A novel dsRNA virus (Cryphonectria carpinicola fusagravirus 1, CcFGV1), isolated from a Japanese strain (JS13) of Cryphonectria carpinicola, was thoroughly characterized. The biological comparison of a set of isogenic CcFGV1-infected and -free (JS13VF) strains indicated asymptomatic infection by CcFGV1. The sequence analysis showed that the virus has a two open reading frame (ORF) genome of 9.6 kbp with the RNA-directed RNA polymerase domain encoded by ORF2. The N-terminal sequencing and peptide mass fingerprinting showed an N-terminally processed or degraded product (150 kDa) of the 5'-proximal ORF1-encoded protein (1462 amino acids) to make up the CcFGV1 spherical particles of ~40 nm in diameter. Interestingly, a portion of CcFGV1 dsRNA co-fractionated with a host protein of 70 kDa. The purified CcFGV1 particles were used to transfect protoplasts of JS13VF as well as the standard strain of an experimental model filamentous fungal host Cryphonectria parasitica. CcFGV1 was confirmed to be associated with asymptomatic infection of both fungi. RNA silencing was shown to target the virus in C. parasitica, resulting in reduced CcFGV1 accumulation by comparing the CcFGV1 content between RNA silencing-competent and -deficient strains. These results indicate the transfectability of spherical particles of a fusagravirus associated with asymptomatic infection.

  59. Mycovirus Hunting Revealed the Presence of Diverse Viruses in a Single Isolate of the Phytopathogenic Fungus Diplodia seriata From Pakistan Peer-reviewed

    Haris Ahmed Khan, Paul Telengech, Hideki Kondo, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Frontiers in Cellular and Infection Microbiology　12　2022/06/29

    Publisher: Frontiers Media SA

    DOI： 10.3389/fcimb.2022.913619 　

    eISSN： 2235-2988

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    Diplodia seriata in the family Botryosphaeriaceae is a cosmopolitan phytopathogenic fungus and is responsible for causing cankers, fruit rot and leaf spots on economically important plants. In this study, we characterized the virome of a single Pakistani strain (L3) of D. seriata. Several viral-like contig sequences were obtained via a previously conducted next-generation sequencing analysis. Multiple infection of the L3 strain by eight RNA mycoviruses was confirmed through RT-PCR using total RNA samples extracted from this strain; the entire genomes were determined via Sanger sequencing of RT-PCR and RACE clones. A BLAST search and phylogenetic analyses indicated that these eight mycoviruses belong to seven different viral families. Four identified mycoviruses belong to double-stranded RNA viral families, including Polymycoviridae, Chrysoviridae, Totiviridae and Partitiviridae, and the remaining four identified mycoviruses belong to single-stranded RNA viral families, i.e., Botourmiaviridae, and two previously proposed families “Ambiguiviridae” and “Splipalmiviridae”. Of the eight, five mycoviruses appear to represent new virus species. A morphological comparison of L3 and partially cured strain L3ht1 suggested that one or more of the three viruses belonging to Polymycoviridae, “Splipalmiviridae” and “Ambiguiviridae” are involved in the irregular colony phenotype of L3. To our knowledge, this is the first report of diverse virome characterization from D. seriata.

  60. Differentiating between viruses and virus species by writing their names correctly Peer-reviewed

    Francisco Murilo Zerbini, Stuart G. Siddell, Arcady R. Mushegian, Peter J. Walker, Elliot J. Lefkowitz, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Bas E. Dutilh, María Laura García, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani

    Archives of Virology　167　(4)　1231-1234　2022/04

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-021-05323-4 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  61. A novel deltapartitivirus from red clover. International-journal Peer-reviewed

    Paul Telengech, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki

    Archives of virology　167　(4)　1201-1204　2022/04

    DOI： 10.1007/s00705-022-05372-3 　

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    The family Partitiviridae has five genera, among which is the genus Deltapartitivirus. We report here the complete genome sequence of a deltapartitivirus from red clover, termed "red clover cryptic virus 3" (RCCV3). RCCV3 has a bisegmented double-stranded (ds) RNA genome. dsRNA1 and dsRNA2 are 1580 and 1589 nucleotides (nt) in length and are predicted to encode an RNA-directed RNA polymerase (RdRP) and a capsid protein (CP), respectively. The RCCV3 RdRP shares the highest sequence identity with the RdRP of a previously reported deltapartitivirus, Medicago sativa deltapartitivirus 1 (MsDPV1) (76.5%), while the RCCV3 CP shows 50% sequence identity to the CP of MsDPV1. RdRP- and CP-based phylogenetic trees place RCCV3 into a clade of deltapartitiviruses. The sequence and phylogenetic analyses clearly indicate that RCCV3 represents a new species in the genus Deltapartitivirus. RCCV3 was detectable in all three tested cultivars of red clover.

  62. Plant viruses and viroids in Japan Invited Peer-reviewed

    Shin-ichi Fuji, Tomofumi Mochizuki, Mitsuru Okuda, Shinya Tsuda, Satoshi Kagiwada, Ken-Taro Sekine, Masashi Ugaki, Keiko T. Natsuaki, Masamichi Isogai, Tetsuo Maoka, Minoru Takeshita, Nobuyuki Yoshikawa, Kazuyuki Mise, Takahide Sasaya, Hideki Kondo, Kenji Kubota, Yasuyuki Yamaji, Toru Iwanami, Kazusato Ohshima, Kappei Kobayashi, Tatsuji Hataya, Teruo Sano, Nobuhiro Suzuki

    Journal of General Plant Pathology　88　(2)　105-127　2022/03

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10327-022-01051-y 　

    ISSN： 1345-2630

    eISSN： 1610-739X

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    Abstract An increasing number of plant viruses and viroids have been reported from all over the world due largely to metavirogenomics approaches with technological innovation. Herein, the official changes of virus taxonomy, including the establishment of megataxonomy and amendments of the codes of virus classification and nomenclature, recently made by the International Committee on Taxonomy of Viruses were summarized. The continued efforts of the plant virology community of Japan to index all plant viruses and viroids occurring in Japan, which represent 407 viruses, including 303 virus species and 104 unclassified viruses, and 25 viroids, including 20 species and 5 unclassified viroids, as of October 2021, were also introduced. These viruses and viroids are collectively classified into 81 genera within 26 families of 3 kingdoms (Shotokuvirae, Orthornavirae, Pararnavirae) across 2 realms (Monodnaviria and Riboviria). This review also overviewed how Japan’s plant virus/viroid studies have contributed to advance virus/viroid taxonomy.

  63. A novel victorivirus from the phytopathogenic fungus Neofusicoccum parvum Peer-reviewed

    Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of Virology　2022/02/03

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-021-05304-7 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  64. A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe Peer-reviewed

    Yukiyo Sato, Sabitree Shahi, Paul Telengech, Sakae Hisano, Carolina Cornejo, Daniel Rigling, Hideki Kondo, Nobuhiro Suzuki

    Virus Research　307　198606-198606　2022/01

    Publisher: Elsevier BV

    DOI： 10.1016/j.virusres.2021.198606 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  65. Assessment of mycoviral diversity in Pakistani fungal isolates revealed infection by 11 novel viruses of a single strain of Fusarium mangiferae isolate SP1. International-journal

    Haris Ahmed Khan, Wajeeha Shamsi, Atif Jamal, Memoona Javaied, Mashal Sadiq, Tehsin Fatma, Aqeel Ahmed, Maleeha Arshad, Mubashra Waseem, Samra Babar, Midhat Mustafa Dogar, Nasar Virk, Hussnain Ahmed Janjua, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

    The Journal of general virology　102　(12)　2021/12

    DOI： 10.1099/jgv.0.001690 　

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    An extensive screening survey was conducted on Pakistani filamentous fungal isolates for the identification of viral infections. A total of 396 fungal samples were screened, of which 36 isolates were found double-stranded (ds) RNA positive with an overall frequency of 9% when analysed by a classical dsRNA isolation method. One of 36 dsRNA-positive strains, strain SP1 of a plant pathogenic fungus Fusarium mangiferae, was subjected to virome analysis. Next-generation sequencing and subsequent completion of the entire genome sequencing by a classical Sanger sequencing method showed the SP1 strain to be co-infected by 11 distinct viruses, at least seven of which should be described as new taxa at the species level according to the ICTV (International Committee on Taxonomy of Viruses) species demarcation criteria. The newly identified F. mangiferae viruses (FmVs) include two partitivirids, one betapartitivirus (FmPV1) and one gammapartitivirus (FmPV2); six mitovirids, three unuamitovirus (FmMV2, FmMV4, FmMV6), one duamitovirus (FmMV5), and two unclassified mitovirids (FmMV1, FmMV3); and three botourmiavirids, two magoulivirus (FmBOV1, FmBOV3) and one scleroulivirus (FmBOV2). The number of coinfecting viruses is among the largest ones of fungal coinfections. Their molecular features are thoroughly described here. This represents the first large virus survey in the Indian sub-continent.

  66. Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand Peer-reviewed

    Sokty Neang, Santiti Bincader, Sansern Rangsuwan, Pisut Keawmanee, Soriya Rin, Lakha Salaipeth, Subha Das, Hideki Kondo, Nobuhiro Suzuki, Ikuo Sato, Daigo Takemoto, Chainarong Rattanakreetakul, Ratiya Pongpisutta, Masao Arakawa, Sotaro Chiba

    Viruses　13　(11)　2269-2269　2021/11/12

    Publisher: MDPI AG

    DOI： 10.3390/v13112269 　

    eISSN： 1999-4915

  67. A new double-stranded rna mycovirus in cryphonectria naterciae is able to cross the species barrier and is deleterious to a new host Peer-reviewed

    Carolina Cornejo, Sakae Hisano, Helena Bragança, Nobuhiro Suzuki, Daniel Rigling

    Journal of Fungi　7　(10)　861　2021/10

    DOI： 10.3390/jof7100861 　

    eISSN： 2309-608X

  68. Changes to virus taxonomy and to the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2021) Peer-reviewed

    Peter J. Walker, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Andrew J. Davison, Donald M. Dempsey, Bas E. Dutilh, María Laura García, Balázs Harrach, Robert L. Harrison, R. Curtis Hendrickson, Sandra Junglen, Nick J. Knowles, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Małgorzata Łobocka, Max L. Nibert, Hanna M. Oksanen, Richard J. Orton, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Dooerslaer, Anne-Mieke Vandamme, Arvind Varsani, Francisco Murilo Zerbini

    Archives of Virology　166　(9)　2633-2648　2021/09

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-021-05156-1 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  69. A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai Peer-reviewed

    Haris Ahmed Khan, Yukiyo Sato, Hideki Kondo, Atif Jamal, Muhammad Faraz Bhatti, Nobuhiro Suzuki

    Archives of Virology　166　2711-2722　2021/07/27

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00705-021-05176-x 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  70. In-tree behavior of diverse viruses harbored in the chestnut blight fungus, Cryphonectria parasitica Peer-reviewed

    Nobuhiro Suzuki, Carolina Cornejo, Annisa Aulia, Sabitree Shahi, Bradley I. Hillman, Daniel Rigling

    Journal of Virology　95　(6)　e01962-20　2021/03

    DOI： 10.1128/JVI.01962-20 　

    ISSN： 0022-538X

    eISSN： 1098-5514

  71. Identification of an RNA silencing suppressor encoded by a symptomless fungal hypovirus, Cryphonectria hypovirus 4 Peer-reviewed

    Annisa Aulia, Kiwamu Hyodo, Sakae Hisano, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Biology　10　(2)　1-16　2021/02

    DOI： 10.3390/biology10020100 　

    eISSN： 2079-7737

  72. Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range Peer-reviewed

    Sabitree Shahi, Sotaro Chiba, Hideki Kondo, Nobuhiro Suzuki

    Virology　554　55-65　2021/02

    DOI： 10.1016/j.virol.2020.11.011 　

    ISSN： 0042-6822

    eISSN： 1096-0341

  73. Identification of a Novel Quinvirus in the Family Betaflexiviridae That Infects Winter Wheat. International-journal Peer-reviewed

    Hideki Kondo, Naoto Yoshida, Miki Fujita, Kazuyuki Maruyama, Kiwamu Hyodo, Hiroshi Hisano, Tetsuo Tamada, Ida Bagus Andika, Nobuhiro Suzuki

    Frontiers in microbiology　12　715545　2021

    DOI： 10.3389/fmicb.2021.715545 　

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    Yellow mosaic disease in winter wheat is usually attributed to the infection by bymoviruses or furoviruses; however, there is still limited information on whether other viral agents are also associated with this disease. To investigate the wheat viromes associated with yellow mosaic disease, we carried out de novo RNA sequencing (RNA-seq) analyses of symptomatic and asymptomatic wheat-leaf samples obtained from a field in Hokkaido, Japan, in 2018 and 2019. The analyses revealed the infection by a novel betaflexivirus, which tentatively named wheat virus Q (WVQ), together with wheat yellow mosaic virus (WYMV, a bymovirus) and northern cereal mosaic virus (a cytorhabdovirus). Basic local alignment search tool (BLAST) analyses showed that the WVQ strains (of which there are at least three) were related to the members of the genus Foveavirus in the subfamily Quinvirinae (family Betaflexiviridae). In the phylogenetic tree, they form a clade distant from that of the foveaviruses, suggesting that WVQ is a member of a novel genus in the Quinvirinae. Laboratory tests confirmed that WVQ, like WYMV, is potentially transmitted through the soil to wheat plants. WVQ was also found to infect rye plants grown in the same field. Moreover, WVQ-derived small interfering RNAs accumulated in the infected wheat plants, indicating that WVQ infection induces antiviral RNA silencing responses. Given its common coexistence with WYMV, the impact of WVQ infection on yellow mosaic disease in the field warrants detailed investigation.

  74. A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix Peer-reviewed

    Juan M. Arjona-López, Paul Telengech, Nobuhiro Suzuki, Carlos J. López-Herrera

    Fungal Biology　125　(1)　69-76　2021/01

    DOI： 10.1016/j.funbio.2020.10.007 　

    ISSN： 1878-6146

  75. Biochemical characterization of the dicing activity of Dicer-like 2 in the model filamentous fungus Neurospora crassa Peer-reviewed

    Midori Tabara, Hisashi Koiwa, Nobuhiro Suzuki, Toshiyuki Fukuhara

    Fungal Genetics and Biology　146　103488　2021/01

    DOI： 10.1016/j.fgb.2020.103488 　

    ISSN： 1087-1845

    eISSN： 1096-0937

  76. Molecular Characterization of a Novel Polymycovirus From Penicillium janthinellum With a Focus on Its Genome-Associated PASrp Peer-reviewed

    Yukiyo Sato, Atif Jamal, Hideki Kondo, Nobuhiro Suzuki

    Frontiers in Microbiology　11　e0045-20　2020/10/20

    DOI： 10.3389/fmicb.2020.592789 　

    eISSN： 1664-302X

  77. Coinfection of Rosellinia necatrix by a partitivirus and a virga-like virus is associated with hypovirulence Peer-reviewed

    Juan M. Arjona-López, Paul Telengech, Nobuhiro Suzuki, Carlos J. López-Herrera

    European Journal of Plant Pathology　158　(1)　111-119　2020/09/01

    DOI： 10.1007/s10658-020-02058-x 　

    ISSN： 0929-1873

    eISSN： 1573-8469

  78. Dicer monitoring in a model filamentous fungus host, Cryphonectria parasitica Peer-reviewed

    Annisa Aulia, Midori Tabara, Paul Telengech, Toshiyuki Fukuhara, Nobuhiro Suzuki

    Current Research in Virological Science　100001-100001　2020/07

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.crviro.2020.100001 　

  79. Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix Peer-reviewed

    Paul Telengech, Sakae Hisano, Cyrus Mugambi, Kiwamu Hyodo, Juan Manuel Arjona-López, Carlos José López-Herrera, Satoko Kanematsu, Hideki Kondo, Nobuhiro Suzuki

    Frontiers in Microbiology　11　1064　2020/06/26

    DOI： 10.3389/fmicb.2020.01064 　

    eISSN： 1664-302X

  80. Virome Analysis of Aphid Populations That Infest the Barley Field: The Discovery of Two Novel Groups of Nege/Kita-Like Viruses and Other Novel RNA Viruses International-journal Peer-reviewed

    Hideki Kondo, Miki Fujita, Hiroshi Hisano, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki

    Frontiers in Microbiology　11　509　2020/04

    DOI： 10.3389/fmicb.2020.00509 　

    eISSN： 1664-302X

  81. Editorial: Frontiers in Fungal Virus Research Peer-reviewed

    Liying Sun, Nobuhiro Suzuki, Daohong Jiang, Massimo Turina, Jiatao Xie

    Frontiers in Cellular and Infection Microbiology　9　456　2020/01/22

    DOI： 10.3389/fcimb.2019.00456 　

    eISSN： 2235-2988

  82. Structure and assembly of double-stranded RNA mycoviruses Peer-reviewed

    Carlos P. Mata, Javier M. Rodríguez, Nobuhiro Suzuki, José R. Castón

    Advances in Virus Research　108　213-247　2020/01

    DOI： 10.1016/bs.aivir.2020.08.001 　

    ISSN： 0065-3527

    eISSN： 1557-8399

  83. Neo-virology: The raison d'etre of viruses International-journal Peer-reviewed

    Tokiko Watanabe, Nobuhiro Suzuki, Keizo Tomonaga, Hirofumi Sawa, Yoshiharu Matsuura, Yasushi Kawaguchi, Hideki Takahashi, Keizo Nagasaki, Yoshihiro Kawaoka

    Virus Research　274　197751-197751　2019/12

    DOI： 10.1016/j.virusres.2019.197751 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  84. ICTV Virus Taxonomy Profile: Megabirnaviridae. International-journal Peer-reviewed

    Sato, Y, Miyazaki, N, Kanematsu, S, Ghabrial, S. A, Hillman, B. I, Suzuki, N, ICTV Report, Consortium

    Journal of General Virology　100　(9)　269-1270　2019/09

    DOI： 10.1099/jgv.0.001297 　

    ISSN： 0022-1317

  85. Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae Peer-reviewed

    Shuhei Ohkita, Yui Lee, Quyet Nguyen, Kenichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

    Virology　534　25-35　2019/08

    DOI： 10.1016/j.virol.2019.05.015 　

    ISSN： 0042-6822

    eISSN： 1096-0341

  86. Isolation and characterization of a novel mycovirus infecting an edible mushroom, Grifola frondosa Peer-reviewed

    Akiko Komatsu, Hideki Kondo, Masayuki Sato, Atsushi Kurahashi, Kozo Nishibori, Nobuhiro Suzuki, Fumihiro Fujimori

    Mycoscience　60　(4)　211-220　2019/07

    DOI： 10.1016/j.myc.2019.01.005 　

    ISSN： 1340-3540

    eISSN： 1618-2545

  87. Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes) Peer-reviewed

    Yu Hsin Lin, Miki Fujita, Sotaro Chiba, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki, Hideki Kondo

    Virology　533　125-136　2019/07

    DOI： 10.1016/j.virol.2019.05.008 　

    ISSN： 0042-6822

    eISSN： 1096-0341

  88. A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing Peer-reviewed

    Annisa Aulia, Ida Bagus Andika, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Virology　533　99-107　2019/07

    DOI： 10.1016/j.virol.2019.05.004 　

    ISSN： 0042-6822

    eISSN： 1096-0341

  89. Novel victorivirus from a pakistani isolate of alternaria alternata lacking a typical translational stop/restart sequence signature Peer-reviewed

    Atif Jamal, Yukiyo Sato, Sabitree Shahi, Wajeeha Shamsi, Hideki Kondo, Nobuhiro Suzuki

    Viruses　11　(6)　577　2019/06

    DOI： 10.3390/v11060577 　

    ISSN： 1999-4915

    eISSN： 1999-4915

  90. Molecular and biological characterization of a novel botybirnavirus identified from a Pakistani isolate of Alternaria alternata International-journal Peer-reviewed

    Wajeeha Shamsi, Yukiyo Sato, Atif Jamal, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

    Virus Research　263　119-128　2019/04/02

    DOI： 10.1016/j.virusres.2019.01.006 　

    ISSN： 0168-1702

  91. A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes. Peer-reviewed

    Kondo H, Chiba S, Maruyama K, Andika IB, Suzuki N

    Virus research　262　37-47　2019/03

    DOI： 10.1016/j.virusres.2017.11.020 　

    ISSN： 0168-1702

  92. "Integration of viral sequences into eukaryotic host genomes: legacy of ancient infections". Peer-reviewed

    Tomonaga K, Suzuki N, Berkhout B

    Virus research　262　1　2019/03

    DOI： 10.1016/j.virusres.2018.12.012 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  93. Hijacking a host scaffold protein, RACK1, for replication of a plant RNA virus. Peer-reviewed

    Hyodo K, Suzuki N, Okuno T

    The New phytologist　221　(2)　935-945　2019/01

    DOI： 10.1111/nph.15412 　

    ISSN： 0028-646X

  94. Investigation of Host Range of and Host Defense against a Mitochondrially Replicating Mitovirus Peer-reviewed

    Sabitree Shahi, Ana Eusebio-Cope, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

    Journal of Virology　93　(6)　2019/01

    DOI： 10.1128/JVI.01503-18 　

    ISSN： 0022-538X

  95. The biological attributes, genome architecture and packaging of diverse multi-component fungal viruses International-journal Peer-reviewed

    Yukiyo Sato, Jos{\'{e, R Cast{\'{o } }n, Nobuhiro Suzuki

    Current Opinion in Virology　33　55-65　2018/12

    DOI： 10.1016/j.coviro.2018.07.009 　

    ISSN： 1879-6257

  96. ICTV Virus Taxonomy Profile: Quadriviridae. Peer-reviewed

    Chiba S, Castón JR, Ghabrial SA, Suzuki N, Ictv Report, Consortium

    The Journal of general virology　99　(11)　1480-1481　2018/11

    DOI： 10.1099/jgv.0.001152 　

    ISSN： 0022-1317

  97. Novel Mitoviruses and a Unique Tymo-Like Virus in Hypovirulent and Virulent Strains of the Fusarium Head Blight Fungus, Fusarium boothii Peer-reviewed

    Mizutani Y, Abraham A, Uesaka K, Kondo H, Suga H, Suzuki N, Chiba S

    Viruses　10　(11)　2018/10

    DOI： 10.3390/v10110584 　

  98. Capsid Structure of dsRNA Fungal Viruses. Peer-reviewed

    Luque D, Mata CP, Suzuki N, Ghabrial SA, Castón JR

    Viruses　10　(9)　2018/09

    DOI： 10.3390/v10090481 　

    ISSN： 1999-4915

  99. ICTV virus taxonomy profile: Hypoviridae Peer-reviewed

    Nobuhiro Suzuki, Said A. Ghabrial, Kook-Hyung Kim, Michael Pearson, Shin-Yi L. Marzano, Hajime Yaegashi, Jiatao Xie, Lihua Guo, Hideki Kondo, Igor Koloniuk, Bradley I. Hillman, Elliot J. Lefkowitz, Andrew J. Davison, Stuart G. Siddell, Sead Sabanadzovic, Donald B. Smith, Richard J. Orton, Peter Simmonds, ICTV Report Consortium

    Journal of General Virology　99　(5)　615-616　2018/05/01

    DOI： 10.1099/jgv.0.001055 　

    ISSN： 1465-2099 0022-1317

  100. Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix: insights into evolutionary biology of fungal viruses Peer-reviewed

    Juan Manuel Arjona-Lopez, Paul Telengech, Atif Jamal, Sakae Hisano, Hideki Kondo, Mery Dafny Yelin, Isabel Arjona-Girona, Satoko Kanematsu, Carlos José Lopez-Herrera, Nobuhiro Suzuki

    Environmental Microbiology　20　(4)　1464-1483　2018/04/01

    DOI： 10.1111/1462-2920.14065 　

    ISSN： 1462-2920 1462-2912

  101. Erratum: A fungal Argonaute interferes with RNA interference (Nucleic acids research (2018) 46 5 (2495-2508))

    Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba V. Vu, Kana Yokoya, Ai Matsubara, Ken-Ichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

    Nucleic acids research　46　(5)　2698　2018/03/16

    Publisher: NLM (Medline)

    DOI： 10.1093/nar/gky078 　

    ISSN： 1362-4962

  102. A fungal Argonaute interferes with RNA interference. International-journal Peer-reviewed

    Nguyen Q, Iritani A, Ohkita S, Vu BV, Yokoya K, Matsubara A, Ikeda KI, Suzuki N, Nakayashiki H

    Nucleic acids research　46　(5)　2495-2508　2018/03

    DOI： 10.1093/nar/gkx1301 　

    ISSN： 0305-1048

    More details Close

    Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and MoAGO3 additively or redundantly played roles in hairpin RNA- and retrotransposon (MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (Δmoago2) showed elevated levels of gene silencing. Consistently, transcript levels of MAGGY and mycoviruses were drastically reduced in Δmoago2, supporting the idea that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis revealed that repeat- and mycovirus-derived small interfering RNAs were mainly associated with MoAGO2 and MoAGO3, and their populations were very similar based on their size distribution patterns and positional base preference. Site-directed mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2 was essential for the ability to diminish the efficacy of RNAi. Overall, these results suggest a possible interplay between distinct sRNA-mediated gene regulation pathways through a competition for sRNA.

  103. First evidence for internal ribosomal entry sites in diverse fungal virus genomes Peer-reviewed

    Sotaro Chiba, Atif Jamal, Nobuhiro Suzuki

    mBio　9　(2)　2018/03/01

    DOI： 10.1128/mBio.02350-17 　

    ISSN： 2150-7511 2161-2129

  104. A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in an unrelated dsRNA virus: Taxonomic and evolutionary considerations Peer-reviewed

    Sakae Hisano, Rui Zhang, Md. Iqbal Faruk, Hideki Kondo, Nobuhiro Suzuki

    Virus Research　244　75-83　2018/01/15

    DOI： 10.1016/j.virusres.2017.11.006 　

    ISSN： 1872-7492 0168-1702

  105. ICTV virus taxonomy profile: Partitiviridae Peer-reviewed

    Eeva J. Vainio, Sotaro Chiba, Said A. Ghabrial, Edgar Maiss, Marilyn Roossinck, Sead Sabanadzovic, Nobuhiro Suzuki, Jiatao Xie, Max Nibert, Elliot J. Lefkowitz, Andrew J. Davison, Stuart G. Siddell, Donald B. Smith, Richard J. Orton, Peter Simmonds, ICTV Report Consortium

    Journal of General Virology　99　(1)　17-18　2018/01/01

    DOI： 10.1099/jgv.0.000985 　

    ISSN： 1465-2099 0022-1317

  106. Viruses of Plant-Interacting Fungi Peer-reviewed

    Bradley I. Hillman, Aulia Annisa, Nobuhiro Suzuki

    Advances in Virus Research　100　99-116　2018/01/01

    DOI： 10.1016/bs.aivir.2017.10.003 　

    ISSN： 1557-8399 0065-3527

  107. Acquisition of functions on the outer capsid surface during evolution of double-stranded RNA fungal viruses Peer-reviewed

    Carlos P. Mata, Daniel Luque, Josue Gomez-Blanco, Javier M. Rodriguez, Jose M. Gonzalez, Nobuhiro Suzuki, Said A. Ghabrial, Jose L. Carrascosa, Benes L. Truss, Jose R. Caston

    PLOS PATHOGENS　13　(12)　e1006755　2017/12

    DOI： 10.1371/journal.ppat.1006755 　

    ISSN： 1553-7366

    eISSN： 1553-7374

  108. A possible occurrence of genome reassortment among bipartite rhabdoviruses Peer-reviewed

    Hideki Kondo, Keisuke Hirota, Kazuyuki Maruyama, Ida Bagus An'dika, Nobuhiro Suzuki

    VIROLOGY　508　18-25　2017/08

    DOI： 10.1016/j.virol.2017.04.027 　

    ISSN： 0042-6822

  109. Roles of superoxide anion and hydrogen peroxide during replication of two unrelated plant RNA viruses in Nicotiana benthamiana Peer-reviewed

    Kiwamu Hyodo, Nobuhiro Suzuki, Kazuyuki Mise, Tetsuro Okuno

    Plant Signaling and Behavior　12　(6)　e1338223　2017/06/03

    DOI： 10.1080/15592324.2017.1338223 　

    ISSN： 1559-2324 1559-2316

  110. Heterodimers as the Structural Unit of the T=1 Capsid of the Fungal Double-Stranded RNA Rosellinia necatrix Quadrivirus 1 Peer-reviewed

    Daniel Luque, Carlos P. Mata, Fernando Gonzalez-Camacho, Jose M. Gonzalez, Josue Gomez-Blanco, Carlos Alfonso, German Rivas, Wendy M. Havens, Satoko Kanematsu, Nobuhiro Suzuki, Said A. Ghabrial, Benes L. Trus, Jose R. Caston

    JOURNAL OF VIROLOGY　90　(24)　11220-11230　2016/12

    DOI： 10.1128/JVI.01013-16 　

    ISSN： 0022-538X

    eISSN： 1098-5514

  111. Reprint of “Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi” Peer-reviewed

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    Virus Research　219　39-50　2016/07/02

    DOI： 10.1016/j.virusres.2016.05.011 　

    ISSN： 1872-7492 0168-1702

  112. The victorivirus Helminthosporium victoriae virus 1905 is the primary cause of disease/hypovirulence in its natural host and a heterologous host (Reprinted from Virus Res., vol 213, pg 238-245, 2016) Peer-reviewed

    Jiatao Xie, Wendy M. Havens, Yu-Hsin Lin, Nobuhiro Suzuki, Said A. Ghabrial

    VIRUS RESEARCH　219　100-107　2016/07

    DOI： 10.1016/j.virusres.2016.05.010 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  113. A novel betapartitivirus RnPV6 from Rosellinia necatrix tolerates host RNA silencing but is interfered by its defective RNAs Peer-reviewed

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    VIRUS RESEARCH　219　62-72　2016/07

    DOI： 10.1016/j.virusres.2015.10.017 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  114. Characterization of a new megabirnavirus that confers hypovirulence with the aid of a co-infecting partitivirus to the host fungus, Rosellinia necatrix Peer-reviewed

    Atsuko Sasaki, Hitoshi Nakamura, Nobuhiro Suzuki, Satoko Kanernatsu

    VIRUS RESEARCH　219　73-82　2016/07

    DOI： 10.1016/j.virusres.2015.12.009 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  115. The world of diverse viruses in the kingdom Fungi Foreword Peer-reviewed

    Nobuhiro Suzuki

    VIRUS RESEARCH　219　1-1　2016/07

    DOI： 10.1016/j.virusres.2016.05.022 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  116. Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi Peer-reviewed

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    VIRUS RESEARCH　213　353-364　2016/02

    DOI： 10.1016/j.virusres.2015.11.015 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  117. The victorivirus Helminthosporium victoriae virus 190S is the primary cause of disease/hypovirulence in its natural host and a heterologous host Peer-reviewed

    Jiatao Xie, Wendy M. Havens, Yu-Hsin Lin, Nobuhiro Suzuki, Said A. Ghabrial

    VIRUS RESEARCH　213　238-245　2016/02

    DOI： 10.1016/j.virusres.2015.12.011 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  118. Large-scale multiplex polymerase chain reaction assay for diagnosis of viral reactivations after allogeneic hematopoietic stem cell transplantation Peer-reviewed

    Natsuko Inazawa, Tsukasa Hori, Naoki Hatakeyama, Masaki Yamamoto, Yuko Yoto, Masanori Nojima, Nobuhiro Suzuki, Norio Shimizu, Hiroyuki Tsutsumi

    JOURNAL OF MEDICAL VIROLOGY　87　(8)　1427-1435　2015/08

    DOI： 10.1002/jmv.24161 　

    ISSN： 0146-6615

    eISSN： 1096-9071

  119. Megabirnavirus structure reveals a putative 120-subunit capsid formed by asymmetrical dimers with distinctive large protrusions Peer-reviewed

    Naoyuki Miyazaki, Lakha Salaipeth, Satoko Kanematsu, Kenji Iwasaki, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY　96　(8)　2435-2441　2015/08

    DOI： 10.1099/vir.0.000182 　

    ISSN： 0022-1317

    eISSN： 1465-2099

  120. Cymbidium chlorotic mosaic virus, a new sobemovirus isolated from a spring orchid (Cymbidium goeringii) in Japan Peer-reviewed

    Hideki Kondo, Shogo Takemoto, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY　160　(8)　2099-2104　2015/08

    DOI： 10.1007/s00705-015-2460-9 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  121. Different Dicer-like protein components required for intracellular and systemic antiviral silencing in Arabidopsis thaliana Peer-reviewed

    Ida Bagus Andika, Kazuyuki Maruyama, Liying Sun, Hideki Kondo, Tetsuo Tamada, Nobuhiro Suzuki

    PLANT SIGNALING & BEHAVIOR　10　(8)　e1039214　2015/08

    DOI： 10.1080/15592324.2015.1039214 　

    ISSN： 1559-2316

    eISSN： 1559-2324

  122. 50-plus years of fungal viruses Peer-reviewed

    Said A. Ghabrial, Jose R. Caston, Daohong Jiang, Max L. Nibert, Nobuhiro Suzuki

    VIROLOGY　479　356-368　2015/05

    DOI： 10.1016/j.virol.2015.02.034 　

    ISSN： 0042-6822

  123. Editorial: Viruses threatening stable production of cereal crops Peer-reviewed

    Nobuhiro Suzuki, Takahide Sasaya, Il-Ryong Choi

    FRONTIERS IN MICROBIOLOGY　6　470　2015/05

    DOI： 10.3389/fmicb.2015.00470 　

    ISSN： 1664-302X

  124. The chestnut blight fungus for studies on virus/host and virus/virus interactions: From a natural to a model host Peer-reviewed

    Ana Eusebio-Cope, Liying Sun, Toru Tanaka, Sotaro Chiba, Shin Kasahara, Nobuhiro Suzuki

    VIROLOGY　477　164-175　2015/03

    DOI： 10.1016/j.virol.2014.09.024 　

    ISSN： 0042-6822

  125. Differential contributions of plant Dicer-like proteins to antiviral defences against potato virus X in leaves and roots Peer-reviewed

    Ida Bagus Andika, Kazuyuki Maruyama, Liying Sun, Hideki Kondo, Tetsuo Tamada, Nobuhiro Suzuki

    PLANT JOURNAL　81　(5)　781-793　2015/03

    DOI： 10.1111/tpj.12770 　

    ISSN： 0960-7412

    eISSN： 1365-313X

  126. Detection and analysis of non-retroviral RNA virus-like elements in plant, fungal, and insect genomes. International-journal Peer-reviewed

    Hideki Kondo, Sotaro Chiba, Nobuhiro Suzuki

    Methods in molecular biology (Clifton, N.J.)　1236　73-88　2015

    DOI： 10.1007/978-1-4939-1743-3_7 　

    ISSN： 1064-3745

    More details Close

    Endogenous non-retroviral RNA like sequences (NRVSs) have been discovered in the genome of a wide range of eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms, and in many cases, those fossils are estimated to be millions-of-years-old. It is likely that the number of NRVS records will increase rapidly due to the growing availability of whole-genome sequences for many kinds of eukaryotes. Discovery of the novel NRVSs and understanding of their phylogenetic relationship with modern viral relatives provide important information on deep evolutionary history of RNA virus-host interactions. In this chapter, therefore, the common strategies for the identification and characterization of endogenous NRVSs from plants, insects, and fungi are described.

  127. Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research Peer-reviewed

    Max L. Nibert, Said A. Ghabrial, Edgar Maiss, Till Lesker, Eeva J. Vainio, Daohong Jiang, Nobuhiro Suzuki

    VIRUS RESEARCH　188　128-141　2014/08

    DOI： 10.1016/j.virusres.2014.04.007 　

    ISSN： 0168-1702

    eISSN： 1872-7492

  128. A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses Peer-reviewed

    Rui Zhang, Shengxue Liu, Sotaro Chiba, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    FRONTIERS IN MICROBIOLOGY　5　360　2014/07

    DOI： 10.3389/fmicb.2014.00360 　

    ISSN： 1664-302X

  129. Biological properties and expression strategy of rosellinia necatrix megabirnavirus 1 analysed in an experimental host, Cryphonectria parasitica Peer-reviewed

    Lakha Salaipeth, Sotaro Chiba, Ana Eusebio-Cope, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY　95　(Pt 3)　740-750　2014/03

    DOI： 10.1099/vir.0.058164-0 　

    ISSN： 0022-1317

    eISSN： 1465-2099

  130. Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus Peer-reviewed

    Hideki Kondo, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    VIROLOGY　452　166-174　2014/03

    DOI： 10.1016/j.virol.2014.01.007 　

    ISSN： 0042-6822

  131. Genome rearrangement of a mycovirus Rosellinia necatrix megabirnavirus 1 affecting its ability to attenuate virulence of the host fungus Peer-reviewed

    Satoko Kanematsu, Takeo Shimizu, Lakha Salaipeth, Hajime Yaegashi, Atsuko Sasaki, Tsutae Ito, Nobuhiro Suzuki

    VIROLOGY　450　308-315　2014/02

    DOI： 10.1016/j.virol.2013.12.002 　

    ISSN： 0042-6822

  132. Complete genome sequence of Habenaria mosaic virus, a new potyvirus infecting a terrestrial orchid (Habenaria radiata) in Japan Peer-reviewed

    Hideki Kondo, Takanori Maeda, I. Wayan Gara, Sotaro Chiba, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY　159　(1)　163-166　2014/01

    DOI： 10.1007/s00705-013-1784-6 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  133. [Cryphonectria parasitica as a host of fungal viruses: a tool useful to unravel the mycovirus world]. Peer-reviewed

    Suzuki N

    Uirusu　64　(1)　11-24　2014

    DOI： 10.2222/jsv.64.11 　

    ISSN： 0042-6857

  134. [Diverse double-stranded RNA viruses infecting fungi]. Peer-reviewed

    Chiba S, Suzuki N

    Uirusu　64　(2)　225-238　2014

    Publisher: The Japanese Society for Virology

    DOI： 10.2222/jsv.64.225 　

    ISSN： 0042-6857

    More details Close

    Most of reported fungal viruses (mycoviruses) have double-stranded RNA (dsRNA) genomes. This may reflect the simple, easy method for mycovirus hunting that entails detection of dsRNAs as a sign of viral infections. There are an increasing number of screens of various fungi, particularly phytopathogenic fungi for viruses pathogenic to host fungi or able to confer hypovirulence to them. This bases on an attractive research field of biological control of fungal plant diseases using viruses (virocontrol), mainly targeting important phytopathogenic fungi. While isolated viruses usually induce asymptomatic symptoms, they show a considerably high level of diversity. As of 2014, fungal dsRNA viruses are classified into six families: Reoviridae, Totiviridae, Chrysoviridae, Partitiviridae, Megabirnaviridae and Quadriviridae. These exclude unassigned mycoviruses which will definitely be placed into distinct families and/or genera. In this review article, dsRNA viruses isolated from the kingdom Fungi including as-yet-unclassified taxa are overviewed. Some recent achievements in the related field are briefly introduced as well.

  135. Nyamiviridae: Proposal for a new family in the order Mononegavirales Peer-reviewed

    Jens H. Kuhn, Sadia Bekal, Yingyun Cai, Anna N. Clawson, Leslie L. Domier, Marieke Herrel, Peter B. Jahrling, Hideki Kondo, Kris N. Lambert, Kathie A. Mihindukulasuriya, Norbert Nowotny, Sheli R. Radoshitzky, Urs Schneider, Peter Staeheli, Nobuhiro Suzuki, Robert B. Tesh, David Wang, Lin-Fa Wang, Ralf G. Dietzgen

    ARCHIVES OF VIROLOGY　158　(10)　2209-2226　2013/10

    DOI： 10.1007/s00705-013-1674-y 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  136. Orchid fleck virus structural proteins N and P form intranuclear viroplasm like structures in the absence of viral infection Peer-reviewed

    Hideki Kondo, Sotaro Chiba, Ida Bagus Andika, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    Journal of Virology　87　(13)　7423-7434　2013/07

    DOI： 10.1128/JVI.00270-13 　

    ISSN： 0022-538X 1098-5514

  137. A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing Peer-reviewed

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY　87　(12)　6727-6738　2013/06

    DOI： 10.1128/JVI.00557-13 　

    ISSN： 0022-538X

  138. A second quadrivirus strain from the phytopathogenic filamentous fungus Rosellinia necatrix Peer-reviewed

    Yu-Hsin Lin, Sakae Hisano, Hajime Yaegashi, Satoko Kanematsu, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY　158　(5)　1093-1098　2013/05

    DOI： 10.1007/s00705-012-1580-8 　

    ISSN： 0304-8608

  139. Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system Peer-reviewed

    Zoe Sanchez, Akio Tani, Nobuhiro Suzuki, Reiko Kariyama, Hiromi Kumon, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING　115　(3)　326-331　2013/03

    DOI： 10.1016/j.jbiosc.2012.09.018 　

    ISSN： 1389-1723

    eISSN： 1347-4421

  140. Effects of Defective Interfering RNA on Symptom Induction by, and Replication of, a Novel Partitivirus from a Phytopathogenic Fungus, Rosellinia necatrix Peer-reviewed

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY　87　(4)　2330-2341　2013/02

    DOI： 10.1128/JVI.02835-12 　

    ISSN： 0022-538X

  141. Evidence for negative-strand RNA virus infection in fungi Peer-reviewed

    Hideki Kondo, Sotaro Chiba, Kazuhiro Toyoda, Nobuhiro Suzuki

    Virology　435　(2)　201-209　2013/01/20

    DOI： 10.1016/j.virol.2012.10.002 　

    ISSN： 0042-6822 1096-0341

  142. Viruses of the white root rot fungus, Rosellinia necatrix. International-journal Peer-reviewed

    Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    Advances in virus research　86　177-214　2013

    DOI： 10.1016/B978-0-12-394315-6.00007-6 　

    ISSN： 0065-3527

  143. A novel quadripartite dsRNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix Peer-reviewed

    Yu-Hsin Lin, Sotaro Chiba, Akio Tani, Hideki Kondo, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    VIROLOGY　426　(1)　42-50　2012/04

    DOI： 10.1016/j.virol.2012.01.013 　

    ISSN： 0042-6822

  144. Mycoreovirus genome alterations: similarities to and differences from rearrangements reported for other reoviruses Peer-reviewed

    Toru Tanaka, Ana Eusebio-Cope, Liying Sun, Nobuhiro Suzuki

    FRONTIERS IN MICROBIOLOGY　3　186　2012

    DOI： 10.3389/fmicb.2012.00186 　

    ISSN： 1664-302X

  145. Rearrangements of mycoreovirus 1 S1, S2 and S3 induced by the multifunctional protein p29 encoded by the prototypic hypovirus Cryphonectria hypovirus 1 strain EP713 Peer-reviewed

    Toru Tanaka, Liying Sun, Kouhei Tsutani, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY　92　(Pt 8)　1949-1959　2011/08

    DOI： 10.1099/vir.0.031138-0 　

    ISSN： 0022-1317

  146. Widespread endogenization of genome sequences of non-retroviral rna viruses into plant genomes

    Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

    PLoS Pathogens　7　(7)　2011/07

    DOI： 10.1371/journal.ppat.1002146 　

    ISSN： 1553-7366

    eISSN： 1553-7374

  147. Rice Dwarf Viruses with Dysfunctional Genomes Generated in Plants Are Filtered Out in Vector Insects: Implications for the Origin of the Virus Peer-reviewed

    Yingying Pu, Akira Kikuchi, Yusuke Moriyasu, Masatoshi Tomaru, Yan Jin, Haruhisa Suga, Kyoji Hagiwara, Fusamichi Akita, Takumi Shimizu, Osamu Netsu, Nobuhiro Suzuki, Tamaki Uehara-Ichiki, Takahide Sasaya, Taiyun Wei, Yi Li, Toshihiro Omura

    JOURNAL OF VIROLOGY　85　(6)　2975-2979　2011/03

    DOI： 10.1128/JVI.02147-10 　

    ISSN： 0022-538X

    eISSN： 1098-5514

  148. [Mycoviruses and virocontrol]. Peer-reviewed

    Chiba S, Kondo H, Kanematsu S, Suzuki N

    Uirusu　60　(2)　163-176　2010/12

    DOI： 10.2222/jsv.60.163 　

    ISSN： 0042-6857

    More details Close

    Viruses are widespread in all major groups of fungi. The transmission of fungal viruses occurs intracellularly during cell division, sporogenesis, and cell fusion. They apparently lack an extracellular route for infection. Recent searches of the collections of field fungal isolates have detected an increasing number of novel viruses and lead to discoveries of novel genome organizations, expression strategies and virion structures. Those findings enhanced our understanding of virus diversity and evolution. The majority of fungal viruses have dsRNA genomes packaged in spherical particles, while ssRNA mycoviruses, possessing or lacking the ability to form particles, have increasingly been reported. This review article discusses the current status of mycovirus studies and virocontrol (biocontrol) of phytopathogenic fungi using viruses that infect them and reduce their virulence. Selected examples of virocontrol-associated systems include the chestnut/chestnut blight/hypovirus and fruit trees/white root rot fungus/mycoviruses. Natural dissemination and artificial introduction of hypovirulent fungal strains efficiently contributed to virocontrol of chestnut blight in European forests. Attempts to control white root rot with hypovirulence-conferring mycoviruses are now being made in Japan.

  149. Complexity in Influenza Virus Targeted Drug Design: Interaction with Human Sialidases Peer-reviewed

    Leonard M. G. Chavas, Ryuichi Kato, Nobuhiro Suzuki, Mark von Itzstein, Maretta C. Mann, Robin J. Thomson, Jeffrey C. Dyason, Jennifer McKimm-Breschkin, Paola Fusi, Cristina Tringali, Bruno Venerando, Guido Tettamanti, Eugenio Monti, Soichi Wakatsuki

    JOURNAL OF MEDICINAL CHEMISTRY　53　(7)　2998-3002　2010/04

    DOI： 10.1021/jm100078r 　

    ISSN： 0022-2623

    eISSN： 1520-4804

  150. Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction Peer-reviewed

    Ana Eusebio-Cope, Liying Sun, Bradley I. Hillman, Nobuhiro Suzuki

    VIROLOGY　397　(2)　399-408　2010/02

    DOI： 10.1016/j.virol.2009.11.035 　

    ISSN： 0042-6822

  151. Overexpression of microRNA395c or 395e affects differently the seed germination of arabidopsis thaliana under stress conditions Peer-reviewed

    Joo Yeol Kim, Hwa Jung Lee, Hyun Ju Jung, Kazuyuki Maruyama, Nobuhiro Suzuki, Hunseung Kang

    Planta　232　(6)　1447-1454　2010

    DOI： 10.1007/s00425-010-1267-x 　

    ISSN： 0032-0935 1432-2048

  152. A Novel Bipartite Double-Stranded RNA Mycovirus from the White Root Rot Fungus Rosellinia necatrix: Molecular and Biological Characterization, Taxonomic Considerations, and Potential for Biological Control Peer-reviewed

    Sotaro Chiba, Lakha Salaipeth, Yu-Hsin Lin, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY　83　(24)　12801-12812　2009/12

    DOI： 10.1128/JVI.01830-09 　

    ISSN： 0022-538X

  153. Characteristics of Hemophagocytic Lymphohistiocytosis in Neonates: A Nationwide Survey in Japan Peer-reviewed

    Nobuhiro Suzuki, Akira Morimoto, Shouichi Ohga, Kazuko Kudo, Yasushi Ishida, Eiichi Ishii

    JOURNAL OF PEDIATRICS　155　(2)　235-238　2009/08

    DOI： 10.1016/j.jpeds.2009.02.050 　

    ISSN： 0022-3476

    eISSN： 1097-6833

  154. Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713 Peer-reviewed

    Li-hua Guo, Liying Sun, Sotaro Chiba, Hiroyuki Araki, Nobuhiro Suzuki

    NUCLEIC ACIDS RESEARCH　37　(11)　3645-3659　2009/06

    DOI： 10.1093/nar/gkp224 　

    ISSN： 0305-1048

  155. Occurrence of the African subgroup (Ia) of BK polyomavirus in younger Japanese children Peer-reviewed

    Kaori Tanaka, Tsukasa Hori, Naoki Hatakeyama, Masaki Yamamoto, Rumiko Takayama, Yuko Yoto, Nobuhiro Suzuki, Toshiaki Hayashi, Yukiho Ikeda, Hiroshi Ikeda, Tadao Ishida, Hiroyuki Tsutsumi

    MICROBIOLOGY AND IMMUNOLOGY　53　(6)　319-322　2009/06

    DOI： 10.1111/j.1348-0421.2009.00129.x 　

    ISSN： 0385-5600

  156. Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica Peer-reviewed

    Ana Eusebio-Cope, Nobuhiro Suzuki, Hamid Sadeghi-Garmaroodi, Masatoki Taga

    FUNGAL GENETICS AND BIOLOGY　46　(4)　342-351　2009/04

    DOI： 10.1016/j.fgb.2009.01.005 　

    ISSN： 1087-1845

  157. Intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus CHV1-EP713 Peer-reviewed

    Liying Sun, Nobuhiro Suzuki

    RNA-A PUBLICATION OF THE RNA SOCIETY　14　(12)　2557-2571　2008/12

    DOI： 10.1261/rna.1125408 　

    ISSN： 1355-8382

  158. Characterization of mutants of the chestnut blight fungus (Cryphonectria parasitica) with unusual hypovirus symptoms Peer-reviewed

    Md. Iqbal Faruk, Masatoshi Izumimoto, Nobuhiro Suzuki

    JOURNAL OF GENERAL PLANT PATHOLOGY　74　(6)　425-433　2008/12

    DOI： 10.1007/s10327-008-0121-5 　

    ISSN： 1345-2630

  159. A host factor involved in hypovirus symptom expression in the chestnut blight fungus, Cryphonectria parasitica Peer-reviewed

    M. Iqbal Faruk, Ana Eusebio-Cope, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY　82　(2)　740-754　2008/01

    DOI： 10.1128/JVI.02015-07 　

    ISSN： 0022-538X

  160. Baculovirus expression of the 11 mycoreovirus-1 genome segments and identification of the guanylyltransferase-encoding segment

    S. Supyani, Bradley I. Hillman, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY　88　342-350　2007/01

    DOI： 10.1099/vir.0.82318-0 　

    ISSN： 0022-1317

  161. Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713 Peer-reviewed

    Liying Sun, Donald L. Nuss, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY　87　3703-3714　2006/12

    DOI： 10.1099/vir0.82213-0 　

    ISSN： 0022-1317

  162. Pns4 of rice dwarf virus is a phosphoprotein, is localized around the viroplasm matrix, and forms minitubules Peer-reviewed

    T. Wei, A. Kikuchi, N. Suzuki, T. Shimizu, K. Hagiwara, H. Chen, T. Omura

    Archives of Virology　151　(9)　1701-1712　2006/09

    DOI： 10.1007/s00705-006-0757-4 　

    ISSN： 0304-8608

  163. The spread of Rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures Peer-reviewed

    Taiyun Wei, Akira Kikuchi, Yusuke Moriyasu, Nobuhiro Suzuki, Takumi Shimizu, Kyoji Hagiwara, Hongyan Chen, Mami Takahashi, Tamaki Ichiki-Uehara, Toshihiro Omura

    JOURNAL OF VIROLOGY　80　(17)　8593-8602　2006/09

    DOI： 10.1128/JVI.00537-06 　

    ISSN： 0022-538X

  164. Pns12 protein of Rice dwarf virus is essential for formation of viroplasms and nucleation of viral-assembly complexes Peer-reviewed

    Taiyun Wei, Takumi Shimizu, Kyoji Hagiwara, Akira Kikuchi, Yusuke Moriyasu, Nobuhiro Suzuki, Hongyan Chen, Toshihiro Omura

    Journal of General Virology　87　(2)　429-438　2006/02

    DOI： 10.1099/vir.0.81425-0 　

    ISSN： 0022-1317

  165. Complete genome sequence of Mycoreovirus-1/Cp9B21, a member of a novel genus within the family Reoviridae, isolated from the chestnut blight fungus Cryphonectria parasitica Peer-reviewed

    N Suzuki, S Supyani, K Maruyama, BI Hillman

    JOURNAL OF GENERAL VIROLOGY　85　3437-3448　2004/11

    DOI： 10.1099/vir.0.80293-0 　

    ISSN： 0022-1317

  166. Viruses of the Chestnut Blight Fungus, Cryphonectria parasitica

    Bradley I. Hillman, Nobuhiro Suzuki

    Advances in Virus Research　63　423-472　2004

    DOI： 10.1016/S0065-3527(04)63007-7 　

  167. A reovirus of the fungus Cryphonectria parasitica that is infectious as particles and related to the Coltivirus genus of animal pathogens Peer-reviewed

    BI Hillman, S Supyani, H Kondo, N Suzuki

    JOURNAL OF VIROLOGY　78　(2)　892-898　2004/01

    DOI： 10.1128/JVI.78.2.892-898.2004 　

    ISSN： 0022-538X

  168. Hypovirus papain-like protease p29 functions in trans to enhance viral double-stranded RNA accumulation and vertical transmission

    N Suzuki, K Maruyama, M Moriyama, DL Nuss

    JOURNAL OF VIROLOGY　77　(21)　11697-11707　2003/11

    DOI： 10.1128/JVI.77.21.11697-11707.2003 　

    ISSN： 0022-538X

  169. Contribution of protein p40 to hypovirus-mediated modulation of fungal host phenotype and viral RNA accumulation

    N Suzuki, DL Nuss

    JOURNAL OF VIROLOGY　76　(15)　7747-7759　2002/08

    DOI： 10.1128/JVI.76.15.7747-7759.2002 　

    ISSN： 0022-538X

  170. Extending chestnut blight hypovirus host range within diaporthales by biolistic delivery of viral cDNA Peer-reviewed

    A Sasaki, M Onoue, S Kanematsu, K Suzaki, M Miyanishi, N Suzuki, DL Nuss, K Yoshida

    MOLECULAR PLANT-MICROBE INTERACTIONS　15　(8)　780-789　2002/08

    DOI： 10.1094/MPMI.2002.15.8.780 　

    ISSN： 0894-0282

  171. Engineering hypoviruses for fundamental and practical applications

    Donald L. Nuss, Baoshan Chen, Lynn M. Geletka, Todd B. Parsley, Nobuhiro Suzuki

    dsRNA Genetic Elements: Concepts and Applications in Agriculture, Forestry, and Medicine　145-163　2001/01/01

    DOI： 10.1201/9781420039122 　

  172. Essential and dispensable virus-encoded replication elements revealed by efforts to develop hypoviruses as gene expression vectors

    N Suzuki, LM Geletka, DL Nuss

    JOURNAL OF VIROLOGY　74　(16)　7568-7577　2000/08

    DOI： 10.1128/JVI.74.16.7568-7577.2000 　

    ISSN： 0022-538X

  173. Similarity and divergence among viruses in the genus Furovirus

    Y Shirako, N Suzuki, RC French

    VIROLOGY　270　(1)　201-207　2000/04

    DOI： 10.1006/viro.2000.0251 　

    ISSN： 0042-6822

  174. Mapping of a hypovirus p29 protease symptom determinant domain with sequence similarity to potyvirus HC-Pro protease

    Nobuhiro Suzuki, Baoshan Chen, Donald L. Nuss

    Journal of Virology　73　9478-9484　1999/10/27

    ISSN： 0022-538X

  175. In vivo and in vitro phosphorylation of rice dwarf phytoreovirus Pns12 cytoplasmic nonstructural protein

    N Suzuki, D Hosokawa, Y Matsuura, A Kikuchi, T Omura

    ARCHIVES OF VIROLOGY　144　(7)　1371-1380　1999

    DOI： 10.1007/s007050050593 　

    ISSN： 0304-8608

  176. The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus

    Masatoshi Tomaru, Wakako Maruyama, Akira Kikuchi, Jin Yan, Yafeng Zhu, Nobuhiro Suzuki, Masamichi Isogai, Yuzuru Oguma, Ikuo Kimura, Toshihiro Omura

    Journal of Virology　71　(10)　8019-8023　1997

    ISSN： 0022-538X

  177. Polycistronic (tri- or bicistronic) phytoreoviral segments translatable in both plant and insect cells

    N Suzuki, M Sugawara, DL Nuss, Y Matsuura

    JOURNAL OF VIROLOGY　70　(11)　8155-8159　1996/11

    ISSN： 0022-538X

  178. Novel NTP binding property of rice dwarf phytoreovirus minor core protein P5

    N Suzuki, T Kusano, Y Matsuura, T Omura

    VIROLOGY　219　(2)　471-474　1996/05

    DOI： 10.1006/viro.1996.0273 　

    ISSN： 0042-6822

  179. DNA cloning, sequence analysis of segment 7 of rice dwarf virus genome and its high expression in E. coli.(共著) Peer-reviewed

    Qu, L, Li, Y, Quan, S, Ding, S, Suzuki, N, Chen, Z

    Acta Microbiologica Sinica　36　335-343　1996

  180. A MAIZE DNA-BINDING FACTOR WITH A BZIP MOTIF IS INDUCED BY LOW-TEMPERATURE

    T KUSANO, T BERBERICH, M HARADA, N SUZUKI, K SUGAWARA

    MOLECULAR & GENERAL GENETICS　248　(5)　507-517　1995/09

    DOI： 10.1007/BF02423445 　

    ISSN： 0026-8925

  181. Molecular Analysis of the Rice Dwarf Phytoreovirus Genome

    SUZUKI Nobuhiro

    Annals of the Phytopathological Society of Japan　61　(3)　176-176　1995/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    DOI： 10.3186/jjphytopath.61.176 　

    ISSN： 0031-9473

  182. Molecular analysis of the rice dwarf virus genome

    Nobuhiro Suzuki

    Seminars in Virology　6　(2)　89-95　1995

    DOI： 10.1006/smvy.1995.0011 　

    ISSN： 1044-5773

  183. Immunodetection of Rice Dwarf Phytoreoviral Proteins in Both Insect and Plant Hosts

    Nobuhiro Suzuki, Mikiko Sugawara, Tomonobu Kusano, Hajime Mori, Yoshiharu Matsuura

    Virology　202　(1)　41-48　1994

    DOI： 10.1006/viro.1994.1320 　

    ISSN： 1096-0341 0042-6822

    eISSN： 1096-0341

  184. NUCLEOTIDE-SEQUENCE OF RICE DWARF PHYTOREOVIRUS GENOME SEGMENT-2 - COMPLETION OF SEQUENCE ANALYSES OF RICE DWARF VIRUS

    UYEDA, I, N SUDA, N YAMADA, H KUDO, K MURAO, H SUGA, KIMURA, I, E SHIKATA, Y KITAGAWA, T KUSANO, M SUGAWARA, N SUZUKI

    INTERVIROLOGY　37　(1)　6-11　1994/01

    DOI： 10.1159/000150348 　

    ISSN： 0300-5526

  185. LOW-TEMPERATURE-DEPENDENT EXPRESSION OF A RICE GENE ENCODING A PROTEIN WITH A LEUCINE-ZIPPER MOTIF

    K AGUAN, K SUGAWARA, N SUZUKI, T KUSANO

    MOLECULAR & GENERAL GENETICS　240　(1)　1-8　1993/07

    DOI： 10.1007/BF00276876 　

    ISSN： 0026-8925

  186. Molecular analysis of rice dwarf phytoreovirus segment S1: Interviral homology of the putative RNA-dependent RNA polymerase between plant- and animal-infecting reoviruses

    Nobuhiro Suzuki, Masako Tanimura, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa, Narushi Suda, Hiroshi Kudo, Ichiro Uyeda, Eishiro Shikata

    Virology　190　(1)　240-247　1992

    DOI： 10.1016/0042-6822(92)91210-L 　

    ISSN： 1096-0341 0042-6822

  187. Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro

    Nobuhiro Suzuki, Mikiko Sugawara, Tomonobu Kusano

    Virology　191　(2)　992-995　1992

    DOI： 10.1016/0042-6822(92)90279-X 　

    ISSN： 1096-0341 0042-6822

  188. ISOLATION OF GENES FOR LOW-TEMPERATURE-INDUCED PROTEINS IN RICE BY A SIMPLE SUBTRACTIVE METHOD

    K AGUAN, K SUGAWARA, N SUZUKI, T KUSANO

    PLANT AND CELL PHYSIOLOGY　32　(8)　1285-1289　1991/12

    ISSN： 0032-0781

  189. MOLECULAR ANALYSIS OF RICE DWARF PHYTOREOVIRUS SEGMENT-S11 CORRESPONDING TO WOUND TUMOR PHYTOREOVIRUS SEGMENT-S12 Peer-reviewed

    N SUZUKI, M HARADA, T KUSANO

    JOURNAL OF GENERAL VIROLOGY　72　2233-2237　1991/09

    ISSN： 0022-1317

  190. Outer capsid protein heterogeneity of rice dwarf phytoreovirus

    N. Suzuki, M. Sugawara

    Journal of General Virology　72　(9)　2239-2242　1991

    DOI： 10.1099/0022-1317-72-9-2239 　

    ISSN： 0022-1317

  191. Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12

    N. Suzuki, M. Harada, T. Kusano

    Journal of General Virology　72　(9)　2233-2237　1991

    DOI： 10.1099/0022-1317-72-9-2233 　

    ISSN： 0022-1317

  192. MOLECULAR-CLONING AND EXPRESSION OF THIOBACILLUS-FERROOXIDANS CHROMOSOMAL RIBULOSE BISPHOSPHATE CARBOXYLASE GENES IN ESCHERICHIA-COLI

    T KUSANO, K SUGAWARA, C INOUE, N SUZUKI

    CURRENT MICROBIOLOGY　22　(1)　35-41　1991/01

    DOI： 10.1007/BF02106210 　

    ISSN： 0343-8651

  193. AN IMPROVED METHOD FOR THE CONSTRUCTION OF HIGH-EFFICIENCY CDNA LIBRARY IN PLASMID OR LAMBDA VECTOR Peer-reviewed

    K AGUAN, T KUSANO, N SUZUKI, Y KITAGAWA

    NUCLEIC ACIDS RESEARCH　18　(4)　1071-1071　1990/02

    DOI： 10.1093/nar/18.4.1071 　

    ISSN： 0305-1048

  194. A simple method for elimination of non-specific reactions in non-precoated and electroblot enzyme-linked immunosorbent assay procedures used for detection of zucchini yellow mosaic virus Peer-reviewed

    Suzuki, N, Shirako, Y, Ehara, Y

    Annals of the Phytopathological Society of Japan　56　337-341　1990

  195. Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa Peer-reviewed

    Nobuhiro Suzuki, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa

    Virology　179　(1)　455-459　1990

    DOI： 10.1016/0042-6822(90)90314-H 　

    ISSN： 1096-0341 0042-6822

  196. Sequence analysis of rice dwarf phytoreovirus genome segments S4, S5, and S6: Comparison with the equivalent wound tumor virus segments Peer-reviewed

    Nobuhiro Suzuki, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa

    Virology　179　(1)　446-454　1990

    DOI： 10.1016/0042-6822(90)90313-G 　

    ISSN： 1096-0341 0042-6822

  197. ISOLATION AND SEROLOGICAL COMPARISON OF VIRUS-CODED PROTEINS OF 3 POTYVIRUSES INFECTING CUCURBITACEOUS PLANTS Peer-reviewed

    N SUZUKI, Y SHIRAKO, Y EHARA

    INTERVIROLOGY　31　(1)　43-49　1990/01

    DOI： 10.1159/000150133 　

    ISSN： 0300-5526

  198. NUCLEOTIDE-SEQUENCE OF RICE DWARF VIRUS SEGMENT-5

    N SUZUKI, Y WATANABE, T KUSANO, Y KITAGAWA

    NUCLEIC ACIDS RESEARCH　17　(21)　8858-8859　1989/11

    DOI： 10.1093/nar/17.21.8858 　

    ISSN： 0305-1048

  199. DISTRIBUTION OF CYLINDRICAL INCLUSION, AMORPHOUS INCLUSION AND CAPSID PROTEINS OF WATERMELON MOSAIC VIRUS-2 IN SYSTEMICALLY INFECTED PUMPKIN LEAVES Peer-reviewed

    N SUZUKI, T KUDO, Y SHIRAKO, Y EHARA, T TACHIBANA

    JOURNAL OF GENERAL VIROLOGY　70　1085-1091　1989/05

    ISSN： 0022-1317

  200. The use of SDS-polyacrylamide gel electrophoresis to assess purification procedure of three potyviruses infecting cucurbitaceous plants. Peer-reviewed

    Suzuki, N, Shirako, Y, Ehara, Y

    Annals of the Phytopathological Society of Japan　55　(5)　586-593　1989

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

    More details Close

    We examined the effects of concentration methods, extraction buffers, clarification reagents, and resuspension buffers on virus yield and purity by using SDS-polyacrylamide gel electrophoresis (SDS-PAGE), so as to establish optimal purification procedures of zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus 2 (WMV2) and the watermelon mosaic virus 1 strain of papaya ringspot virus (PRSV-W). We then investigated the physicochemical properties of the three viruses. SDS-PAGE proved very useful to monitor virus purification in a small scale. The three viruses were purified with similar yields of 5-10 mg per 100 g of infected pumpkin leaves by the respective optimal methods. The procedures basically involved clarification with Triton X-100, differential centrifugation using a 20% sucrose pad, and 20-50% sucrose density gradient centrifugation. From WMV2 and PRSV-W purified preparations, capsid protein (CP) of 34,000 (34K) was predominantly detected in addition to minor degradation products of 27K to 33K, respectively. A breakdown product of 28K was, however, as detectabel as intact CP of 33K from ZYMV purified preparation. The RNAs of WMV2 and PRSV-W, which were a little larger than ZYMV RNA, were indistinguishable in size as evaluated with 1.7% agarose gel electrophoresis in denaturing conditions.

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    鈴木信弘

    ネオウイルス学（河岡義裕編）集英社　2021/03

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    佐藤有希代, SHAMSI W., SHAMSI W., JAMAL A., BHATTI M.F., 近藤秀樹, 鈴木信弘

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    近藤秀樹, 藤田美貴, 久野裕, 兵頭究, 鈴木信弘

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    植物病理学第２版（眞山滋志・土佐幸雄編）文永堂　2020/03

  14. Molecular characterization of a novel phenui-like mycovirus in the cultivated mushroom, Lentinula edodes

    藤田美貴, LIN Y-H., 鈴木信弘, 近藤秀樹

    日本植物病理学会大会プログラム・講演要旨予稿集　2020　2020

  15. Inter-colony horizontal transmission of a capsidless 11-segmented RNA virus in Fusarium oxysporum: Are all segments transmitted?

    佐藤有希代, SHAMSI W., SHAMSI W., JAMAL A., BHATTI M. F., 近藤秀樹, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2020　2020

  16. 栽培シイタケから見いだされた新規菌類マイナス鎖RNAウイルスの塩基配列解析

    近藤秀樹, LIN Y.-H., 藤田美貴, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2019　2019

  17. Fusarium boothii病原性衰退株BL13に感染する新規Tymovirales目ウイルスの性状解析

    水谷行善, ABRAHAM Adane, 上坂一馬, 近藤秀樹, 須賀晴久, 鈴木信弘, 千葉壮太郎

    日本植物病理学会大会プログラム・講演要旨予稿集　2019　2019

  18. ネオライフスタイル(裸性)を持つ可能性がある新規ポリマイコウイルスの同定

    佐藤有希代, SHAMSI W., SHAMSI W., JAMAL A., JAMAL A., BHATTI M. F., 近藤秀樹, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2019　2019

  19. 植物ウイルス感染の抗糸状菌植物免疫への影響

    兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2019　2019

  20. 宿主足場タンパク質RACK1のハイジャックによる植物ウイルス増殖機構

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会報　85　(1)　2019

    ISSN： 0031-9473

  21. Characterization of diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix

    MICHENI C. M., TELENGECH P. K., HISANO S., KONDO H., ARJONA-LOPEZ J. M., LOPEZ-HERRERA C., KANEMATSU S., SUZUKI N.

    日本植物病理学会大会プログラム・講演要旨予稿集　2018　2018

  22. 宿主足場タンパク質RACK1は植物RNAウイルスの増殖を正に制御する

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会大会プログラム・講演要旨予稿集　2018　2018

  23. Frontiers in fungal virology

    Nobuhiro Suzuki

    Journal of General Plant Pathology　83　(6)　419-423　2017/11/01

    DOI： 10.1007/s10327-017-0740-9 　

    ISSN： 1345-2630

    eISSN： 1610-739X

  24. アカクローバーうどんこ病菌より見いだされた新規トティウイルス

    近藤秀樹, 久野昌, 千葉壮太郎, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2017　2017

  25. 植物ラブドウイルスの分節化と進化

    近藤秀樹, 鈴木信弘

    日本進化学会大会プログラム・講演要旨集(Web)　19th　2017

  26. 温度依存的に蓄積するGrifola frondosa RNA virus1(GfRV1)がマイタケ宿主遺伝子群の発現に及ぼす影響

    小松あき子, 近藤秀樹, 佐藤真之, 鈴木信弘, 藤森文啓

    日本微生物生態学会大会(Web)　2017　ROMBUNNO.O‐019 (WEB ONLY)　2017

  27. 食用キノコの子実体形成にウイルス感染が果たす役割についての研究(3)

    小松あき子, 佐藤真之, 佐藤真之, 佐藤真之, 近藤秀樹, 西堀耕三, 鈴木信弘, 藤森文啓, 藤森文啓

    東京家政大学生活科学研究所研究報告　39　57‐61-61　2016/07

    Publisher: 東京家政大学生活科学研究所

    ISSN： 0914-5192

  28. RNAサイレンシング活性化による異種菌類ウイルス間の干渉作用(II)

    LIN Y.-H., 千葉壮太郎, 千葉壮太郎, 近藤秀樹, 兼松聡子, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2016　2016

  29. Diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix

    TELENGECH P. K., HISANO S., KONDO H., KANEMATSU S., SUZUKI N.

    日本植物病理学会大会プログラム・講演要旨予稿集　2016　2016

  30. 活性酸素種は植物RNAウイルス増殖を正に制御する

    兵頭究, 鈴木信弘, 奥野哲郎

    日本植物病理学会大会プログラム・講演要旨予稿集　2016　2016

  31. マイタケに感染するマイコウイルスの機能解析研究

    小松あき子, 佐藤真之, 近藤秀樹, 鈴木信弘, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)　39th　ROMBUNNO.1P‐0009 (WEB ONLY)　2016

  32. Advances in Fungal Virus Research Invited Peer-reviewed

    SUZUKI Nobuhiro

    Virus Research　219　1-180　2016

  33. Functional Analysis of Viral Infection in the Fruit-Body Formation of Edible Mushroom(2)

    Komatsu Akiko, Sato Masayuki, Kondo Hideki, Sumi Mariko, Tsuchiya Yuki, Kurahashi Atsushi, Nishibori Kozo, Suzuki Nobuhiro, Fujimori Fumihiro

    東京家政大学生活科学研究所研究報告 = Bulletin of Research Institute of Domestic Science, Tokyo Kasei University　38　79-84　2015/07

    Publisher: 東京家政大学生活科学研究所

    ISSN： 0914-5192

    More details Close

    東京家政大学生活科学研究所研究報告は、本研究所の本年度の活動成果を取りまとめたものです。本研究報告の内容の一部は、別途学会誌等に発表されることがありますのでご了承ください。

  34. RNAサイレンシングとDI-RNAによるパルティティウイルスRnPV6病原性・複製への影響

    千葉壮太郎, 千葉壮太郎, LIN Y.-H., 近藤秀樹, 兼松聡子, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2015　2015

  35. Deep sequencing解析により明らかになったBotrytis tulipaeの9種ウイルスによる混合感染

    近藤秀樹, 久野昌, LIN Y. H., 千葉壮太郎, 千葉壮太郎, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2015　2015

  36. マイタケから見出されたマイコウイルスの性状解析

    小松あき子, 佐藤真之, 角真理子, 下田隆史, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓

    日本生化学会大会(Web)　88th　2015

  37. マイタケ内在マイコウイルスが宿主の形質に及ぼす影響の解析

    佐藤真之, 小松あき子, 下田隆史, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓

    日本きのこ学会大会講演要旨集　19th　2015

  38. 宿主ゲノム上に存在する RNA ウイルス感染記録を紐解く. Invited

    近藤秀樹, 千葉壮太郎, 鈴木信弘

    植物感染生理談話会論文集　50　133-142　2015

  39. Functional Analysis of Viral Infection in the Fruit-Body Formation of Edible Mushroom

    Komatsu Akiko, Kondo Hideki, Sato Masayuki, Tsuchiya Yuki, Kurahashi Atsushi, Nishibori Kozo, Suzuki Nobuhiro, Fujimori Fumihiro

    東京家政大学生活科学研究所研究報告 = Bulletin of Research Institute of Domestic Science, Tokyo Kasei University　37　109-114　2014/07

    Publisher: 東京家政大学生活科学研究所

    ISSN： 0914-5192

    More details Close

    東京家政大学生活科学研究所研究報告は、本研究所の本年度の活動成果を取りまとめたものです。本研究報告の内容の一部は、別途学会誌等に発表されることがありますのでご了承ください。

  40. 2分節マイナス鎖RNAウイルスであるランえそ斑紋ウイルス(OFV)のmRNAおよびleader RNAの特性

    近藤秀樹, 丸山和之, 千葉壮太郎, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2014　2014

  41. 糸状菌におけるデュアルルシフェラーゼ実験系の確立とマイコウイルスIRESエレメントの同定

    千葉壮太郎, 近藤秀樹, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2014　2014

  42. Rosellinia necatrix fusarivirus 1:白紋羽病菌から分離された新規RNAウイルス

    ZHANG Rui, LIU Shengxue, 千葉壮太郎, 近藤秀樹, 兼松聡子, 鈴木信弘

    日本植物病理学会報　80　(1)　2014

    ISSN： 0031-9473

  43. 植物および昆虫の核ゲノム上に見いだされたベニウイルス様配列

    近藤秀樹, 千葉壮太郎, 鈴木信弘

    日本植物病理学会報　80　(1)　2014

    ISSN： 0031-9473

  44. マイタケPartitivirus GfPV1の性状と生物学的特性に関する研究

    小松あき子, 佐藤真之, 佐藤真之, 佐藤真之, 土屋有紀, 土屋有紀, 倉橋敦, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓, 藤森文啓

    日本菌学会大会講演要旨集　57th　2013

  45. 新規ビクトリウイルスの自然および実験宿主菌への人工接種で明らかになったウイルス-宿主間相互作用

    千葉壮太郎, LIN Y.H., 近藤秀樹, 兼松聡子, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2013　2013

  46. DI-RNAと宿主RNAサイレンシングは菌類パルティティウイルスの複製を抑制する

    LIN Y.-H., 千葉壮太郎, 近藤秀樹, 兼松聡子, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2013　2013

  47. マイナス鎖RNAウイルスの菌類への感染の可能性

    近藤秀樹, 千葉壮太郎, 豊田和弘, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2013　2013

  48. 新規ビクトリウイルスの宿主域拡大とウイルス・宿主相互作用の解析

    千葉壮太郎, LIN Yu-Hsin, 近藤秀樹, 兼松聡子, 鈴木信弘

    日本ウイルス学会学術集会プログラム・抄録集　61st　2013

  49. 新規benyvirus・burdock mottle virus(BdMoV)と植物・昆虫ゲノム上のbenyvirus類似配列の同定

    近藤秀樹, 千葉壮太郎, ANDIKA Ida Bagus, 鈴木信弘, 玉田哲男

    日本ウイルス学会学術集会プログラム・抄録集　61st　2013

  50. Rosellinia necatrix(白紋羽病菌)から分離された新規RNAウイルス

    ZHANG Rui, LIU Shengxue, 千葉壮太郎, 近藤秀樹, 兼松聡子, 兼松聡子, 鈴木信弘

    日本ウイルス学会学術集会プログラム・抄録集　61st　2013

  51. Defective interfering(DI)RNAのRosellinia necatrix partitivirus2複製への影響

    LIU Yu-Hsin, 千葉壮太郎, 近藤秀樹, 兼松聡子, 鈴木信弘

    日本植物病理学会報　79　(1)　2013

    ISSN： 0031-9473

  52. マイタケに存在する新規partitivirusの性状と生物学的特性に関する研究

    小松あき子, 佐藤真之, 佐藤真之, 佐藤真之, 角真理子, 土屋(内田)有紀, 倉橋敦, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)　36th　2013

  53. マイタケに発見された新規RNAウイルスの性状と生物学的特性に関する研究

    角真理子, 佐藤真之, 小松あき子, 土屋(内田)有紀, 倉橋敦, 近藤秀樹, 鈴木信弘, 西堀耕三, 藤森文啓

    日本分子生物学会年会プログラム・要旨集(Web)　36th　2P-0014 (WEB ONLY)　2013

  54. 白紋羽病菌とクリ胴枯病菌のマイコウイルス―病原力低下因子とヴァイロコントロール―農作物生産におけるウイルスの有効利用 Invited

    鈴木 信弘

    JATAFF ジャーナル　1　4-8　2013

  55. Hypovirus Cysteine Proteases p29 and p48

    Nobuhiro Suzuki

    Handbook of Proteolytic Enzymes　2　2192-2195　2013

    Publisher: Elsevier Ltd

    DOI： 10.1016/B978-0-12-382219-2.00492-0 　

  56. マイナス鎖RNAウイルスの菌類への感染の可能性

    近藤秀樹, 千葉壮太郎, 鈴木信弘

    日本ウイルス学会学術集会プログラム・抄録集　60th　2012

  57. 植物の核ゲノム上に見いだされるマイナス鎖RNAウイルス様配列

    近藤秀樹, 千葉壮太郎, 梅林絵里, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2012　2012

  58. 植物ゲノムへ水平伝播された太古のパルティティウイルス配列

    千葉壮太郎, 近藤秀樹, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本植物病理学会報　78　(1)　2012

    ISSN： 0031-9473

  59. Cymbidium chlorotic mosaic virusはソベモウイルス属の新規ウイルス種である

    近藤秀樹, 前田孚憲, 鈴木信弘

    日本植物病理学会報　77　(1)　2011

    ISSN： 0031-9473

  60. 非レトロRNAウイルス由来の植物染色体配列

    近藤秀樹, 千葉壮太郎, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2011　2011

  61. 2La13 Establishment and application of a method for classification of Methylobacterium sp. by MALDI-TOF/MS

    Tani Akio, Okumura Marie, Kimbara Kazuhide, Suzuki Nobuhiro

    63　198-198　2011

    Publisher: 日本生物工学会

  62. Family Reoviridae. Invited

    Attoui, H, Mertens, P.P.C, Becnel, J, Belaganahalli, S, Bergoin, M, Brussaard, C.P, Chappell, J.D, Ciarlet, M, del Vas, M, Dermody, T.S, Dormitzer, P.R, Duncan, R, Fcang, Q, Graham, R, Guglielmi, K.M, Harding, R.M, Hillman, B, Makkay, A, Marzachì, C, Matthijnssens, J, Milne, R.G, Mohd Jaafar, F, Mori, H, Noordeloos, A.A, Omura, T, Patton, J.T, Rao, S, Maan, M, Stoltz, D, Suzuki, N, Upadhyaya, N.M, Wei, C, Zhou, H

    Virus Taxonomy: Ninth Report of the International Committee for the Taxonomy of Viruses.　2011

    Publisher: Elsevier Academic Press

  63. ランえそ斑紋ウイルス(OFV)の核内viroplasm形成にはNおよびP蛋白質が必要である

    近藤秀樹, 野田瑞紀, 廣門知紗, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2010　2010

  64. サギソウモザイクウイルスRNAの全塩基配列の決定

    丸山和之, 近藤秀樹, 前田孚憲, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集　2010　2010

  65. 非パラレトロRNAウイルス由来の植物染色体遺伝子

    千葉壮太郎, 近藤秀樹, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

    日本ウイルス学会学術集会プログラム・抄録集　58th　2010

  66. シロイヌナズナDicer-like遺伝子の変異がPotato virus X(PVX)の蓄積に及ぼす影響

    ANDIKA I. B., 近藤秀樹, 鈴木信弘

    日本植物病理学会報　75　(3)　2009

    ISSN： 0031-9473

  67. 徳島県のシンビジウムから分離されたランえそ斑紋ウイルス(OFV)の塩基配列の解析

    近藤秀樹, 野田瑞紀, 広田恵介, 前田孚憲, 玉田哲男, 鈴木信弘

    日本植物病理学会報　75　(3)　2009

    ISSN： 0031-9473

  68. ランえそ斑紋ウイルス(OFV)のヌクレオキャプシドタンパク質遺伝子(N)を用いた分子系統解析

    近藤秀樹, 野田瑞紀, 前田孚憲, 玉田哲男, 鈴木信弘

    日本植物病理学会報　75　(1)　2009

    ISSN： 0031-9473

  69. Potato virus X(PVX)サブゲノムRNAの蓄積はRNAサイレンシングにより根特異的に抑制される

    ANDIKA I. B., 近藤秀樹, 玉田哲男, 鈴木信弘

    日本植物病理学会報　75　(1)　2009

    ISSN： 0031-9473

  70. Viral and host factors involved in symptom induction and replication of RNA viruses infecting phytopathogenic fungi.

    Salaipeth, L, Lin, Y.-H, Eusebio-Cope, A, Tanaka, T, Chiba, S, Suzuki, N

    Proceeding of the International Symposium on Fungal Genetics and Genomics　92-94　2009

  71. (265) Induction of Mycoreovirus 1 Genome Rearrangements by the Multifunctional Protein p29 of the Prototype Hypovirus CHV1-EP713(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Suzuki N., Sun L.-Y.

    Annals of the Phytopathological Society of Japan　74　(3)　237-237　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  72. (286) Soil-borne Virus-encoded Cysteine-rich Proteins Suppress RNA Silencing in Roots(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Andika I.B., Kondo H., Tamada T., Suzuki N.

    Annals of the Phytopathological Society of Japan　74　(3)　243-243　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  73. (266) Assembly of Orchid Fleck Virus-like Particles in Nuclei is Associated with Formation of Viroplasm-like Structure(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kondo H., Noda M., Tamada T., Suzuki N.

    Annals of the Phytopathological Society of Japan　74　(3)　237-238　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  74. (32)Breakdown of RNA Silencing-mediated Resistance in BNYVV 54 kDa-RTD ORF Transgenic Plants by Internal Deletion of 54 kDa-RTD in the Viral Genome(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Andika I. B., Kondo H., Tamada T., Suzuki N.

    Annals of the Phytopathological Society of Japan　74　(1)　64-64　2008/02/20

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  75. (31)The Pathogenicity of M Structural Protein of Orchid Fleck Virus in Nicotiana benthamiana is Suppressed by the Interaction with Nucleocapsid Protein N(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Kondo H., Tamada T., Suzuki N.

    Annals of the Phytopathological Society of Japan　74　(1)　64-64　2008/02/20

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  76. ランえそ斑紋ウイルスの構造タンパク質が誘導する核内viroplasm様領域と粒子形成との関連性

    近藤秀樹, 廣門知紗, 玉田哲男, 鈴木信弘

    日本ウイルス学会学術集会プログラム・抄録集　56th　2008

  77. 土壌伝染性ウイルスBNYVVおよびTRVは根におけるRNAサイレンシングを効果的に抑制する

    ANDIKA Ida Bagus, 近藤秀樹, 玉田哲男, 鈴木信弘

    日本ウイルス学会学術集会プログラム・抄録集　56th　2008

  78. Fungal viruses Invited

    Ghabrial, S, Suzuki, N

    Encyclopedia of Virology 3rd Edition　2　284-291　2008

    Publisher: Elsevier

  79. (229) Characterization of Strains of the Chestnut Blight Fungus, Cryphonectria Parasitica that are Silenced for Transgenes(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Maruyama K., Suzuki N.

    Annals of the Phytopathological Society of Japan　73　(3)　236-236　2007/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  80. (372) The Spread oi Rice dwarf virus among Cells of Its Insect Vector Exploits Virus-induced Tubular Structures(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Omura T., Wei Taiyun, Kikuchi A., Moriyasu Y., Suzuki N., Shimizu T., Hagiwara K., Chen Hongyan, Takahashi M., Ichiki-Uehara T.

    Annals of the Phytopathological Society of Japan　72　(4)　301　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  81. (370) Pns4 of Rice dwarf virus Is a Phosphoprotein, Is Localized around the Viroplasm Matrix, and Forms Minitubules(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Hagiwara K., Wei Taiyun, Kikuchi A., Suzuki N., Shimizu T., Chen Hongyan, Omura T.

    Annals of the Phytopathological Society of Japan　72　(4)　300-301　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  82. (341) Further Functional Analysis of the Papain-like Protease Encoded by Cryphonectria Hypovirus 1(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Sun L. Y., Maruyama K., Suzuki N.

    Annals of the Phytopathological Society of Japan　71　(3)　276-276　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  83. マイコウイルス学の新展開 Invited

    SUZUKI Nobuhiro

    144-157　2005

    Publisher: ソフトサイエンス社

  84. Family Hypoviridae. Invited

    Nuss, D. L, Hillman, B. I, Rigling, D, Suzuki, N

    Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.　597-601　2005

    Publisher: Academic Press

  85. Genus Mycoreovirus Invited

    Mertens, P. P. C, Wei, C, Suzuki, N, Hillman, B. I

    Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.　556-560　2005

  86. Functinal Domains of Papain-like Protease p29 Encoded by the Prototype Hypovirus CHV1-EP713(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Suzuki N., Maruyama K.

    Annals of the Phytopathological Society of Japan　69　(3)　315-316　2003/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  87. ウイルスプロテアーゼ。

    鈴木信弘

    化学と生物 学会出版センター刊　41, 183-192.　(5)　335-344　2003

    Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry

    DOI： 10.1271/kagakutoseibutsu1962.41.335 　

    ISSN： 0453-073X

  88. (210)Resistance to BNYVV in Transgenic Plants with 54 k Protein Coding Domain Is Mediated by the RNA Silencing Mechanism

    Andika I.B., Kondo H., Suzuki N., Tamada T.

    Annals of the Phytopathological Society of Japan　68　(2)　212-212　2002/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  89. ウイルスをもってカビを制す

    鈴木 信弘

    化学と生物 (日本農芸化学会編)　36　524-526　1998

  90. (312) Immunogold Localization of Rice Dwarf Virus Proteins, Pns4 and Pns10, Expressed by Recombinant Baculoviruses in Insect Cells(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    HOSOKAWA D., SUZUKI N.

    Annals of the Phytopathological Society of Japan　63　(3)　268-269　1997/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  91. (311) Phosphorylation and Intracellular Localization of a Rice Dwarf Phytoreovirus Nonstructural Protein, Pns12(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    SUZUKI N., HOSOKAWA D., MATSUURA Y., SHIRAKO Y., KIKUCHI A., OMURA T.

    Annals of the Phytopathological Society of Japan　63　(3)　268-268　1997/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  92. Structure/function analysis of rice dwarf phytoreovirus genome and its proteins.

    Suzuki Nobuhiro

    Uirusu　47　(2)　145-154　1997

    Publisher: The Japanese Society for Virology

    DOI： 10.2222/jsv.47.145 　

    ISSN： 0042-6857

  93. Purification of Rice Dwarf Phytoreoviral Proteins Highly Expressed in Spodoptera frugiperda Cells

    SUZUKI N., SUGAWARA M.

    Annals of the Phytopathological Society of Japan　62　(6)　600-600　1996/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  94. Immunogold Localization of Rice Dwarf Phytoreovirus-coded Nonstructural Proteins in Infected Barley Cells

    HOSOKAWA D., SUZUKI N.

    Annals of the Phytopathological Society of Japan　62　(3)　339-339　1996/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  95. Polycistronic Phytoreovirus Genome Segment

    SUZUKI N., SUGAWARA M., NUSS D.L., MATSUURA Y.

    Annals of the Phytopathological Society of Japan　62　(3)　339-339　1996/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  96. Detection of Rice Dwarf Phytoreovirus RNA by RT-PCR

    KOBAYASHI J., KONDOH N., KATSUMOTO K., SUZUKI N., MIYAJIMA S.

    Annals of the Phytopathological Society of Japan　61　(3)　281-281　1995/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  97. Characterization of Rice Dwarf Phytoreovirus P5

    SUZUKI N., SUGAWARA M., KUSANO T., MATSUURA Y., OMURA T.

    Annals of the Phytopathological Society of Japan　61　(3)　281-281　1995/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  98. A Structural Protein Encoded by Rice Dwarf Phytoreovirus Segment S5

    SUZUKI N, SUGAWARA M

    Annals of the Phytopathological Society of Japan　60　(6)　763-763　1994/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  99. Quantitative Detection of Rice Dwarf Phytoreovirus-Coded Proteins in Both Plant and Insect Hosts

    SUZUKI N., MORI H., SUGAWARA M., KUSANO T., MATSUURA Y.

    Annals of the Phytopathological Society of Japan　60　(3)　382-383　1994/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  100. Immunodetection of Rice Dwarf Phytoreovirus Nonstructural Proteins in Both Infected Rice and Leafhopper

    SUZUKI N., SUGAWARA M., KUSANO T., MATSUURA Y.

    Annals of the Phytopathological Society of Japan　59　(6)　763-764　1993/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  101. Rice Dwarf Phytoreovirus S12 Transcript is Tricistronic in vitro

    SUZUKI N., SUGAWARA M., KUSANO T.

    Annals of the Phytopathological Society of Japan　59　(3)　342-343　1993/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  102. Detection of Rice Dwarf Phytoreovirus-Coded Proteins in Viruliferous Leafhoppers

    SUZUKI N., MORI H., SUGAWARA M.

    Annals of the Phytopathological Society of Japan　59　(3)　328-329　1993/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  103. Molecular Analysis of Rice Dwarf Phytoreovirus Segment S1 : Interviral Homo1ogy of the Putative RNA-Dependent RNA Polymerase between Plant- and Animal-Infecting Reoviruses

    SUZUKI S., TANIMURA M., WATANABE Y., KUSANO T., KITAGAWA Y., SUDA N., KUDO H., UYEDA I., SHIKATA E.

    Annals of the Phytopathological Society of Japan　58　(4)　630-631　1992/10/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  104. Reactivities of Monoclonal Antibodies to Cylindrical Inclusion, Amorphous Inclusion, and Capsid Proteins of Watermelon Mosaic Virus 2 with Corresponding Proteins of Other Potyviruses

    SUZUKI Nobuhiro, SHIRAKO Yukio, EHARA Yoshio

    Annals of the Phytopathological Society of Japan　57　(5)　706-710　1991/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  105. Molecular analysis of rice dwarf phytoreovirus.

    Seminars in Virology　6　89-95

  106. Virus Taxonomy : Seventh Report of the International Committee for the Taxonomy of Viruses.(共著)

    M.H.V. van Regenmortel et al., eds. Academic Press　395-480

  107. Fungal disease control by viruses.

    Kagaku-to-seibutu. Gakkai Shuppan Sentah

  108. Structure/function analysis of rice swarf phytoreovirus genome and its protein.

    Virus　47　145-154

    DOI： 10.2222/jsv.47.145 　

  109. Virus Taxonomy : Seventh Report of the International Committee for the Taxonomy of Viruses.(共著)

    M.H.V. van Regenmortel et al., eds. Academic Press　395-480

Show all ︎Show first 5

Books and Other Publications 22

  1. ゲノム近傍の RNA 編集酵素・転写因子遺伝子に よる真菌の抗ウイルス応答の制御

    本田信治, 鈴木信弘

    実験医学　2025/09

  2. Go viral.

    Hatvani, L., Suzuki, N., Fitzpatrick, D., Grogan, H.

    TResearch Spring Volume 20, Number 1　2025/04

  3. 国際ウイルス分類委員会による植物ウイルスの分類と命名

    鈴木信弘

    植物防疫　2025/02

  4. 別種のウイルスから「殻」を借りる：「ヤドカリ」ウイルスのなぞ

    鈴木信弘

    国立科学博物館 Milsil 「ミルシル」　2024/05

  5. ネオウイルス学

    河岡, 義裕, 川口, 寧, 高橋, 英樹, 澤, 洋文, 朝長, 啓造, 松浦, 善治, 堀江, 真行, 佐藤, 佳, 鈴木, 信弘, 長崎, 慶三, 中川, 草, 野田, 岳志, 村田, 和義, 牧野, 晶子, 渡辺, 登喜子, 望月, 智弘, 大場, 靖子, 西浦, 博, 岩見, 真吾, 古瀬, 祐気

    集英社　2021/03

    ISBN: 9784087211597

  6. Structure of dsRNA Mycoviruses. Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Caston, J. R, Suzuki, N, Ghabrial, S. A

    Academic Press, Elsevier　2021/03

  7. Megabirnaviruses (Megabirnaviridae). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Sato, Y, Suzuki, N

    Academic Press, Elsevier　2021/03

  8. Quadriviruses (Quadriviridae). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Kondo, H, Caston, J. R, Suzuki, N

    Academic Press, Elsevier　2021/03

  9. Yado-kari virus 1 and yado-nushi virus 1 (Unassigned). Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Das, S, Suzuki, N

    Academic Press, Elsevier　2021/03

  10. An introduction to fungal viruses. Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman).

    Suzuki, N.

    2021/03

  11. Viruses as Infectious Agents: Fungal, invertebrate, bacterial archaeal viruses Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman)

    Suzuki, N, Krell, P, Feiss, M, Prangishvili, M

    Academic Press, Elsevier　2021/03

  12. 植物病理学第２版（眞山滋志・土佐幸雄編）

    鈴木信弘・大木理・上田一郎

    文永堂　2020/03

  13. Integration of viral sequences into eukaryotic host genomes: legacy of ancient infections

    2019/03

  14. Advances in Fungal Virus Research

    2016

  15. Viruses threatening stable production of cereal crops.

    2015

  16. Ninth Report of the International Committee for the Taxonomy of Viruses. A. M. Q. King et al., eds.

    Attoui, H., Mertens, P.P.C., Becnel, J., Belaganahalli, S., Bergoin, M., Brussaard, C.P., Chappell, J.D., Ciarlet, M., del Vas, M., Dermody, T.S., Dormitzer, P.R., Duncan, R., Fcang, Q., Graham, R., Guglielmi, K.M., Harding, R.M., Hillman, B., Makkay, A., Marzachì, C., Matthijnssens, J., Milne, R.G., Mohd Jaafar, F., Mori, H., Noordeloos, A.A., Omura, T., Patton, J.T., Rao, S., Maan, M., Stoltz, D., Suzuki, N., Upadhyaya, N.M., Wei, C. and Zhou, H.

    Elsevier, Academic Press　2011

  17. The Springer index of viruses

    Tidona, Christian A, Darai, Gholamreza

    Springer　2011

    ISBN: 9780387959184

  18. Encyclopedia of Virology 3rd Edition,

    Elsevier　2008

  19. Virus Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses.

    Academic Press, San Diego.　2005

  20. 作物保護の新展開ﾑバイオサイエンスのかけはしー

    ソフトサイエンス社　2005

  21. "Fungal dsRNA elements: concepts and application in agriculture, forestry and medicine."

    CRC Press. Boca Raton, Florida, USA.　2001

  22. Virus Taxonomy: Seventh Report of the International Committee for the Taxonomy of Viruses.

    Academic Press, NY.　2000

Show all Show first 5

Presentations 60

  1. From neovirology to phytopathology.

    Suzuki, N

    The first Chinese Mycovirus Symposium　2023/11/25

  2. Yadokari/yadonushi nature: A virus in a virus in a fungus in a plant. Invited

    Sato, Y, Suzuki, N

    The Second International Molecular Plant Protection Congress. Turkey.　2023/05/15

  3. Experimental and practical control measures for white root rot in fruit trees in Japan. Invited

    Suzuki, N

    Yangling International Agri-science Forum.

  4. Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous? Invited

    Suzuki, N

    Awaji International Forum on Immunity and Infection.

  5. Dual functionality of fungal Dicer in multilayer antiviral defense. International-presentation Invited

    SUZUKI Nobuhiro

    International Symposium on Virus Diseases of Important Crops.　2019/09

  6. Dicer functions transcriptionally and post-transcriptionally in a multilayer antiviral defense International-presentation Invited

    Suzuki, N, Andika, I. B, Kondo, H

    European Congress of Virology 2019　2019/05

  7. Another nude virus: a capsidless ssRNA virus hosted by an unrelated dsRNA virus. International-presentation Invited

    SUZUKI Nobuhiro

    13th International dsRNA Virus Symposium　2018/09

  8. Viruses as biological control (virocontrol) agents of plant fungal pathogens. Invited

    Suzuki, N., and Kanematsu, S.

    The First International Congress of Biological Control.　2018/05

  9. A neo-virus-lifestyle exhibited by a (+)RNA virus hosted by an unrelated dsRNA virus in a phytopathogenic fungus. Invited

    Suzuki, N

    The Spring Meeting of the Korean Society of Plant Pathology (KSPP 2018)　2018/04/26

  10. A neo-virus-lifestyle exhibited by a (+)RNA virus hosted in an unrelated dsRNA virus Invited

    N. Suzuki.

    The 16th Awaji International Forum on Infection and Immunity.　2017/09

  11. Mutualist Interactions between a Capsidless (+) ssRNA Virus and a dsRNA Virus Occurring in a Phytopathogenic Fungus. Invited

    Suzuki, N.

    ASM Microbe 2017　2017/06

  12. Fungal RNA silencing involved in antiviral defense and RNA recombination. Invited

    Suzuki, N

    ASV 2016 Annual Meeting　2016/06/18

  13. Antiviral defense in the phytopathogenic filamentous ascomycete, Cryphonectria parasitica. Invited

    Suzuki, N

    Swiss Society for Microbiology Annual Assembly and Meeting　2016/06/14

  14. Another nude virus: a capsidless ssRNA virus hosted by an unrelated dsRNA virus. Invited

    Suzuki, N

    International Symposium on Virology and Fungal Genetics　2016/01/15

  15. Highly activated RNA silencing via strong induction of dicer by one virus can interfere with the replication of an unrelated virus. Invited

    Chiba, S, Suzuki, N

    BMB2015 Biochemistry and Molecular Biology　2015/12/02

  16. A new virus lifestyle challenging the virus rules and concepts. Invited

    A new virus, lifestyle challenging the virus rules, concepts

    JICA Africa-ai-Japan Project/JSPS-AASP Sponsored Workshop on Plant Science and Resource Innovative Research Core with Pan African University.　2015/10/19

  17. From virology to plant disease control. Invited

    Suzuki, N

    JSPS Core-to-Core Program Sponsored Workshop on Crop Science and Innovation for Agriculture.　2015/10/15

  18. A new virus life style challenging the virus rules and concepts. Invited

    Zhang, R, Hisano, S, Tani, A, Kondo, H, Kanematsu, S, Suzuki, N

    13th Spanish Virology Congress　2015/06/08

  19. Virus interference in plants and fungal hosts. Invited

    Suzuki N

    9th JKUAT Scientific and Technological Conference 2014　2014/11/13

  20. The chestnut blight fungus for studies on virus/host and virus/virus interactions: from a natural to a model host. Invited

    Suzuki N

    EMBO Conference Viruses of microbes: Structure and function, from molecules to communities.　2014/07/16

  21. Transcription factor ZAO1 plays a key role in antiviral symptom mitigation in Cryphonectria parasitica.

    Zhang, Z., Honda, S., Suzuki, N.

    The 6th International Mycovirus Symposium　2026/06/11

  22. Myco-immunity: Sophisticated antiviral defense in filamentous fungi. Invited

    Suzuki, N.

    The Third Chinese Mycovirus Symposium　2025/11/22

  23. Viruses spread without borders: Cross-kingdom infections in agroecosystems. Invited

    Kondo, H., Andika, I. B., Sun, L., Suzuki, N.

    Symposium: Plant viruses and viroids on the move: Old routes, new insights. The 71st Annual Meeting of the Japanese Society for Virology　2025/10/25

  24. モデル生物アカパンカビでの抗ウイルス「環境応答」で連動するクロマチン制御とRNA編集機構 Invited

    本田 信治, 横山 歩, 鈴木 信弘

    第97回日本遺伝学会 ワークショップ 「菌類・酵母のクロマチン制御と環境応答」　2025/09/10

  25. ヴァイロコントロール：ウイルスをもってカビを制す

    鈴木 信弘

    第 45 回糸状菌遺伝子研究　2025/06/25

  26. Effects of several mycoviruses on gene expression in different isolates of Cryphonectria parasitica

    Benito-Delgado, A., Pastor-Durántez, E., del Campo-Calvo, R., Suzuki, N., Diez, J.J.

    6th International Mycovirus Symposium　2025/06/11

  27. RNA editing of genomic neighbors controls antiviral response in fungi.

    Honda, S., Yokoyama, A., Suzuki, N.

    The 6th International Mycovirus Symposium　2025/06/11

  28. A capsidless (+)RNA yadokarivirus hosted by a dsRNA virus is infectious as particles, cDNA, and dsRNA.

    Fadli, M., Hisano, S., Novoa, G., Castón, J. R., Kondo, H., Suzuki, N.

    The 6th International Mycovirus Symposium　2025/06/11

  29. Virus-mediated transposon regulation in the chestnut blight fungus.

    Sato, Y., Suzuki, N.

    The 6th International Mycovirus Symposium　2025/06/11

  30. Thorough characterization of a new curvulavirid from Japanese strains of Cryphonectria nitschkei.

    Shahi, S., Hisano, S., Sa'diyah, W., Takaki, Y. Kondo, H., Suzuki, N.

    The 6th International Mycovirus Symposium　2025/06/11

  31. Mycoviruses in Aspergillus luchuensis isolated from fermentation environments in Japan.

    Kondo, H., Nanaji, M., Fujita, M., Sugahara, H., Suzuki, N., Fujimori, F.

    The 6th International Mycovirus Symposium　2025/06/11

  32. Cryo-EM structure of yadonushi/yadokari viruses in Rosellinia necatrix and Aspergillus foetidus. Invited

    Castón, J. R., Novoa, G., Suzuki, N.

    The 6th International Mycovirus Symposium,　2025/06/11

  33. A capsidless (+)RNA yadokarivirus hosted by a dsRNA virus is infectious as particles, cDNA, and dsRNA

    Muhammad Fadli, Sakae Hisano, Guy Novoa, José, R. Castón, Hideki Kondo, Nobuhiro Suzuki

    16th PSJ Plant Virus Disease Workshop: Interface between plant and fungal viruses III,　2025/03/29

  34. Evidence for the replication of a bipartite plant rhabdovirus in its arthropod mite vector.

    Kondo, H., Hyodo, K., Suzuki, N.

    The 5th Korea-Japan Joint Symposium on Plant Pathology　2025/03/25

  35. Co-option of host V-ATPase and autophagy pathways for the replication of a plant RNA virus.

    Hyodo, K., Kondo, H., Suzuki, N.

    The 5th Korea-Japan Joint Symposium on Plant Pathology　2025/03/25

  36. Infection of mycovirus FbLFV1 and its defective RNA induces hypovirulence by a loss of fungal membrane integrity in Fusarium boothii.

    Abbhi, V., Sato, Y., Mizutani, Y., Sato, I., Takemoto, D., Kondo, H., Suzuki, N., Chiba, S.

    The 5th Korea-Japan Joint Symposium on Plant Pathology.　2025/03/25

  37. The World of Fungal Viruses Invited

    Nobuhiro SUZUKI

    Rob Goldbach Lecture Event at Wageningen University　2024/10/31

  38. Virocontrol measures for phylopathogenic fungi. Invited

    Suzuki, N

    Special seminar series for phytopathology.　2023/11/23

  39. Autophagy promotes the replication of red clover necrotic mosaic virus.

    Hyodo, K, Sudo, K, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2020. June 24-28, 2023.　2023/06/25

  40. Possible yadonusi/yadokari nature observed in a Japanese strain NBRC 4031 of Aspergillus foetidus.

    Fadli, M, Hisano, S, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2023　2023/06/26

  41. Replication of single partitiviruses in hosts across three kingdoms; Fungi, Plantae and Animalia

    Telengech, P, Hyodo, K, Ichikawa, H, Kondo, H, Suzuki, N

    The 42nd Annual Meeting of the American Society for Virology 2023　2023/06/25

  42. Viruses in the causal agents of dry bubble disease: Novel candidates for biological candidates for biological pest management.

    Lóránt Hatvani, Hideki Kondo, Nobuhiro Suzuki, Sándor Kocsubé, Helen Grogan

    Mushroom Days Brabanthallen 's-Hertogenbosch　2023/05/11

  43. Structure and assembly of fungal dsRNA viruses

    Gil-Cantero1, D, Novoa, G, Martínez-Romero, J. M, Luque, D, Nobuhiro Suzuki, N, Castón, J. R

    8th European Congress of Virology.　2023/05/05

  44. Mycoviruses: A novel approach for controlling mushroom pathogenic fungi.

    Hatvani, L, Kondo, H, Shahi, S, Suzuki, N, Grogan, H

    Power of Fungi and Mycotoxins in Climate Change　2022/09/16

  45. Fusagraviruses in Cryphonectria species

    Carolina Cornejo, Sandra Gaehwiler, Wajeeha Shamsi, Hideki Kondo, Nobuhiro Suzuki, Daniel Rigling

    The fifth International Mycovirus Symposium　2022/06/01

  46. Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous? Invited

    Yukiyo Sato, Sakae Hisano, Carlos Lopez Hererra, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium　2022/05/31

  47. A transfectable fusagravirus from Cryphonectria carpinicola with spherical particles

    Sakae Hisano, Subha Das, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium.　2022/06/01

  48. Argonaute-independent antiviral RNA interference in the chestnut blight fungus, Cryphonectria parasitica.

    Yukiyo Sato, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium. Gargnano,　2022/05/31

  49. Low-level IRES activity likely contributes to the replication restriction of Cryphonectria hypovirus 1 in Cryphonectria carpinicola Invited

    Sabitree Shahi, Sotaro Chiba, Yoshihiro Takaki, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium　2022/05/31

  50. A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe

    Paul Telengech, Yukiyo Sato, Sabitree Shahi, Sakae Hisano, Carolina Cornejo, Daniel Rigling, Hideki Kondo, Nobuhiro Suzuki

    The fifth International Mycovirus Symposium　2022/06/01

  51. Synonymous codon usage bias in Cryphonectria parasitica viruses: Insights on evolution and host adaptation Invited

    Zeeshan Zahid, Alanna B. Cohen, Nobuhiro Suzuki, Bradley I. Hillman

    The fifth International Mycovirus Symposium　2022/06/01

  52. Partnership between capsidless (+)ssRNA yadokariviruses and their host dsRNA viruses: Promiscuous or virtuous?

    Yukiyo Sato, Sakae Hisano, Subha Das, Carlos Lopez Hererra, Hideki Kondo, Nobuhiro Suzuki

    The 40th Annual Meeting of the American Society for Virology 2020.

  53. Diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix.

    Telengech, P, Hisano, S, Micheni, C. M, Hyodo, K, Arjona-Lopez, J.M, Lopez-Herrera, C, Kanematsu, S, Kondo, H, Suzuki, N

    The 40th Annual Meeting of the American Society for Virology 2020.

  54. Prevalence, diversity and impact of mycoviruses infecting plant pathogenic fungi pf rice in the Philippines.

    Guinto, T, Cope, A, Urzo, M, Budot, B, Yanoria, M. J, Jonson, G, Oliva, R, Kondo, H, Suzuki, N

    Philippine Phytopathological Society Pest Management Council of the Philippines, Inc. PMCP 2021

  55. 2A-mediated co-translational cleavage is prerequisite for the viability of yado-kari virus 1 hosted by yado-nushi virus 1.

    Das, S, Alam, M, Zhang, R, Hisano, S, Sato, Y, Kondo, H, Suzuki, N

    The 39th Annual Meeting of the American Society for Virology 2020

  56. Maintenance and loss of dispensable genomic segments of a polymyco-like capsidless fungal virus with an eleven-segmented RNA genome during its infection cycle.

    Sato, Y, Shamsi, W, Jamal3, A, Bhatti, M. F, Kondo, H, Suzuki, N

    The 39th Annual Meeting of the American Society for Virology 2020

  57. In-tree behavior of diverse viruses infecting the chestnut blight fungus.

    Suzuki, N, Aulia, A, Shahi, S, Hillman, B. I, Cornerjo, C, Rigling, D

    The 39th Annual Meeting of the American Society for Virology 2020.

  58. Mycoviruses in C. parasitica and their biological control potential. International-presentation Invited

    SUZUKI Nobuhiro

    WSL Colloquium.　2019/10/30

  59. Vigorous induction of dicer-like 2 gene by a virus interferes with an unrelated virus in the model phytopathogenic fungus Cryphonectria parasitica. Invited

    Chiba, S, Suzuki, N

    12th PSJ Plant Virus Disease Workshop:The Interface between plant and fungal viruses II.　2016/03/14

  60. A new virus life style challenging the virus rules and concepts. Invited

    R. Zhang, S. Hisano, A. Tani, H. Kondo, S. Kanematsu, N. Suzuki

    63rd Annual Meeting of the Japanese Society for Virology　2015/11/23

Show all Show first 5

Research Projects 43

  1. Exploration of vertically transmitted RNA viruses and establishment of a novel gene expression system in insects

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: National Agriculture and Food Research Organization

    2026/04/01 - 2031/03/31

  2. 菌類免疫研究で実現する多層性抗ウイルス機構の深層理解と植物免疫との接点

    鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(S)

    Institution: 岡山大学

    2026/04/01 - 2031/03/31

  3. ネオウイルス学から現代ウイルス学への挑戦

    鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(開拓)

    Institution: 岡山大学

    2025/06/27 - 2029/03/31

  4. 作物超個体における根圏RNAウイルス叢の実体解明とその生態学的役割

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 岡山大学

    2023/04/01 - 2027/03/31

  5. Studies on the rhizospheric virome of crop holobiants and its ecological roles

    Offer Organization: JSPS (Japan Society for the Promotion of Science

    2023/04 - 2027/03

  6. Antiviral symptom mitigation pathway conserved in ascomycetes and plants

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for JSPS Fellows

    Institution: Okayama University

    2024/04/23 - 2026/03/31

  7. Frontiers in myco-immunity research and new development in relevant phytopathology

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (S)

    Institution: Okayama University

    2021/07/05 - 2026/03/31

  8. Platform construction for research into viruses infecting phytopathogenic obligate fungi and challenge for their biological control

    Offer Organization: JSPS (Japan Society for the Promotion of Science

    2023/06 - 2026/03

  9. ３つの生物界を跨いだウイルス感染

    鈴木 信弘, TELENGECH PAUL

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 特別研究員奨励費

    Institution: 岡山大学

    2023/03/08 - 2025/03/31

  10. Virus infection across the three kingdoms Plantae, Fungi, and Animalia: defense/counter-defense and host adaptation

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Pioneering)

    Institution: Okayama University

    2021/07/09 - 2025/03/31

  11. Frontier in mycoimmunity research and its new developments in phytopathology

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Okayama University

    2021/04/05 - 2025/03/31

  12. Infection across three kingdoms by single viruses

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows

    Category: Grant-in-Aid for JSPS Fellows

    Institution: Okayama University

    2022/07 - 2025/03

  13. 宿主アカパンカビの抗ウイルス免疫暴走化機序の解明

    本田 信治, 鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 福井大学

    2021/04/01 - 2024/03/31

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    本研究代表らは構築したモデル糸状菌アカパンカビのウイルス感染系（Honda et al, Nature Comm, 2020）を用いて、宿主がウイルス感染に対して過剰に免疫応答することで、病気になることを発見した。本研究の目的は、この免疫暴走化に関わる「ウイルス認識機構とその下流因子」、免疫暴走の鍵となる「病的原因遺伝子」を同定し、その分子機序を解明することである。 これまでの解析により、アカパンカビのDICERであるDCL-2を欠損させると、免疫暴走化が抑制されて病気から回復することを見出している。この結果から、DCL-2が有するdsRNA結合部位がウイルスのゲノムdsRNAを認識し、下流の免疫暴走の鍵となる「病的原因遺伝子」の転写を活性化すると考えた。そこで、研究計画に沿って種々の機能変異DCL-2を有する形質変換株を作製し、解析を行った。その結果、DCL-2のdsRNA結合部位、及び、DICER活性が免疫暴走化に関与することを確認した。 また、クリ胴枯病菌ではSAGA複合体がウイルス応答遺伝子群の転写活性化に関与していることが報告されていることから（Andika et al, PNAS, 2017）、SAGA複合体を介する転写活性化と免疫暴走化の関係を調査した。その結果、DCL-2欠損と同様に、SAGA複合体の構成因子を欠損させると、免疫暴走化が抑制されることから、アカパンカビとクリ胴枯病菌に共通するウイルス応答機構の存在が示唆された。

  14. In-tree behavior of diverse viruses harbored in the chestnut blight fungus Cryphonectria parasitica and their biocontrol potential

    Offer Organization: JSPS

    2022/04 - 2024/03

  15. Investigation into the potential role of fungal viruses in the dry bubble disease of cultivated mushroom – towards biological pest management

    System: European Commission (EU) Research Leaders 2025/Marie Skłodowska Curie Fellowship (2022-2025)

    2022/04 - 2023/10

  16. モデル糸状菌アカパンカビを用いたウイルス研究フロンティア

    近藤 秀樹, 鈴木 信弘, 本田 信治

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 岡山大学

    2021/07/09 - 2023/03/31

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    本課題では、菌類ウイルス学のモデル実験系を構築するため、代表的な実験系糸状菌の一種であるアカパンカビを用いてウイルス学を展開するための基盤整備を進めている。以下に本年度の研究実績を示す。 柱① アカパンカビの感染性ウイルスを用いたウイルス学：先の報告で見出されたアカパンカビに自然感染する新規ウイルスの性状解析を進を進めている（Hondaら, Nature Comm 2020、未発表）。これまでに、Neurospora crassaのNGSデータでデルタフレキシ及び新奇RNAウイルス(配列断片）が見出された菌株をついて、感染ウイルスのゲノム配列解析を進めている。さらに、Neurospora intermediaのNGS解析で見出されたウイルス様コンテイグ（フザリウイルス、ミトウイルス、デルタフレキシウイルス）について、元菌株のRT-PCRによりその存在確認と部分配列の決定をおこなった。一部ウイルスについては、3'RLM-RACEによる末端配列を進めている。これらのウイルスのRdRP配列に基づく分子系統樹(最尤法)を作成し、系統学的位置付けを検討した。 柱② ウイルス病徴発現・複製、防御機構/ウイルス反撃に関与する因子の同定と機構解明：N. crassaの抗ウイルスRNAi機構の鍵酵素であるdcl1, dcl2 qde2の単独欠損変異株(dcl1、dcl2, qde2)、二重欠損変異株（dcl1/dcl2, qde2/dcl1, qde2/dcl2）、三重欠損変異体（qde2/dcl1/dcl2）および野生型を用いて、異なるゲノムタイプの二種マイコウイルス(一本鎖RNAウイルスと分節型dsRNAウイルス）の混合感染株をそれぞれ作出した。これらの混合感染株について全RNAを抽出し、小分子RNA分画のRNA-seq解析を行った。現在，得られたRNA-seqの配列データの詳細解析を進めている。

  17. Host range determinants of a virocontrol agent for the chestnut blight disease

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows

    Category: Grant-in-Aid for JSPS Fellows

    Institution: Okayama University

    2021/04/28 - 2023/03/31

  18. 作物根圏におけるウイルス叢の多様性とその感染動態から紐解く生態的意義

    近藤 秀樹, 久野 裕, 鈴木 信弘, 兵頭 究

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 岡山大学

    2020/04/01 - 2023/03/31

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    本課題では、ムギ類生存圏(特に根系)におけるウイルス叢の多様性・普遍性や動態を紐解き、さらにそれらの未知なる生態学的役割の一端に迫ることを目指している。以下に本年度の研究実績を示す。 柱①根圏に存在する主要な菌類・昆虫ウイルス群の宿主探索：初年度に引き続き、ムギ類（オオムギ・コムギ）の地上部・根系、さらにアブラムシ、うどんこ病菌などのRNAseqデータを取得した。得られたデータセットはウイルス配列の確認作業や分子系統解析を進めている。さらに、北海道のコムギサンプルより見出された新規フレキシウイルス（WVQ）の解析の結果，当該圃場に三系統が存在し、ライムギにも感染できること、さらに土壌伝染性ウイルスの可能性が高いことを見出している。根系ウイルス叢のリザーバー探索については、本年度はオオムギ根のRNAseqデータに含まれる微生物リードの抽出と配列相同性解析により、微生物叢の概要を調査した。 柱②根圏ウイルスの生物界を跨ぐ水平伝搬ポテンシャルと宿主への影響：昨年度より継続中のルテオウイルスなど新規植物ウイルスのゲノム解析を進めており、RT-PCRおよびRLM-RACE解析により全長ゲノムRNA配列を確認・決定した。また、圃場のうどんこ病菌のウイルスについては、今年度はdsRNAを鋳型にした全長cDNAのPCR増幅法の検討を進め、そのアンプリコン配列解析により未同定のゲノム末端配列の解析を進めている。 柱③根圏ウイルス叢の年次変動とムギ類ウイルスの病原性の(再)評価：核酸抽出を伴わない簡易ウイルス診断法（RT-PCRをベースとする）の条件を設定し、一部圃場サンプル(葉)でルテオウイルス等の検出を進めている。現在、圃場オオムギサンプル（保存している過年度のを含む）について網羅的に検定すすめている。また、アブラムシ媒介性新規植物ウイルスについては、チャンバー内試験にて病原性評価を進めている。

  19. ネオウイルス学：生命の源流から超個体、そしてエコ・スフィアーへ

    河岡 義裕, 朝長 啓造, 澤 洋文, 松浦 善治, 川口 寧, 渡辺 登喜子, 鈴木 信弘, 高橋 英樹, 長崎 慶三

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東京大学

    2021/04/01 - 2022/03/31

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    「ネオウイルス学」領域内の計画研究および公募研究で得られた各班それぞれの研究テーマについて研究成果を取りまとめ、さらに班間、あるいは、「共進化」、「共生」、「多様性」ユニット間の連携テーマの重要性・意義を考慮しつつ、領域全体の研究成果を取りまとめた。業績集を作成し、冊子体として印刷して領域内外で配布することによって、本領域の研究成果を発信した。報告内容は、出来るだけ分かりやすい記述を心がけ、学術分野だけでなく、一般国民にも読んでもらえるような業績集を作成した。また、高校生、大学生、一般の国民に向けた講演会やセミナー、そして領域ホームページとTwitterによって、領域の活動内容や研究成果を報告することによって、国民に向けた研究成果の発信に努めた。また、新しい試みとして「ウイルスおりがみ」を作成した。ウイルスおりがみは、おりがみを折ることで、一般国民にウイルスの面白さを知ってもらうことを目的とした、科学コミュニケーションツールであり、公募研究班の牧野晶子助教（京都大）、デザイン・ユニットCOCHAE、合同会社SHIBUYA PUBLISHING & BOOKSELLERS、三宅文子アート・プランナーらのグループによって作成された。ウイルスおりがみには領域内の研究者による解説も加えられており、ウイルスの基本、構造の面白さ、役に立つウイルス、生き物の中にいる多様なウイルスについて学ぶことができる。ウイルスおりがみの配布の公募をしたところ2288部の応募があり、抽選で500部を無償で配布した。日本デザイン振興会が主催するグッドデザイン賞へウイルスおりがみを出品中である。

  20. Frontier of research on antiviral immunity in phytopathogenic filamentous fugi

    Suzuki Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Okayama University

    2017/04/01 - 2021/03/31

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    The objective of this project is to explore RNA silencing (RNAi) against fungal viruses in plant pathogenic filamentous fungi. A great progress has been made, leading to several breakthroughs: 1) Discovery of RNAi mediated antiviral defense that requires Dicer but not AGO in the chestnut blight fungus, 2) Identification of the SAGA complex as a transcriptional regulator of fungal RNA silencing, 3) Discovery of a novel antiviral defense mechanism by which virus-induced symptom expression is alleviated by transcriptional upregulation of many host genes upon virus infection in C. parasitica, and 4) Identification of Dicer and SAGA (universal transcriptional coactivator) as the key transcriptional regulators in the new defense, indicating the dual role of Dicer at the post-transcriptional and transcriptional levels. These findings will bring about a paradigm shift in RNAi. Fungi are now being established as the third position next to animals and plants in antiviral defense research.

  21. Neo-virology: the raison d'etre of viruses

    Kawoaka Yoshihiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: The University of Tokyo

    2016/06/30 - 2021/03/31

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    We have established a new academic field, designated as ‘Neo-virology’, in which we define a virus as a component of the global ecosystem and aim to elucidate its key roles in host organisms and the global ecosystem. We have constructed a system to support research activities, facilitate collaborations among research groups, develop human resources, plan and manage scientific meetings, and support public relations activities, all of which have resulted in accelerating this new field of research. Our efforts have produced numerous noteworthy publications and have helped develop many young investigators, who will continue the growth of Neo-Virology into the future.

  22. Neo-lifestyle of fungal viruses

    Suzuki Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Okayama University

    2016/06/30 - 2021/03/31

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    The objective of this project is to establish two neo-lifestyles of fungal viruses and explore their generality and ecological significance. First, we have proved the yadokari/ yadonushi nature in Japanese strains of Rosellinia necatrix and revealed its molecular basis. Capsidless yadokari virus 1 (YkV1) highjacks the capsid of yadnushi virus 1 (YnV1) to replicate in the heterocapsid using its own RNA-dependent RNA polymerase, while in return YkV1 trans-enhances the replication of YnV1. For the hadaka nature, we have shown that hadaka virus 1 (HadV1) from a Pakistani strain of Fusarium oxysporum exists as a capsidless naked form at least in mycelial homogenates that is accessible by RNase A and unable to be pelleted by untracentrifugation. While the yadonushi/yadokari nature has been observed not only foreign fungal strains but also in plants,the hadaka nature has also been detected in other filamentous fungi, suggesting suggesting the generality of the two viral neo-lifestyles.

  23. 「ネオウイルス学」の国際活動支援

    河岡 義裕, 朝長 啓造, 澤 洋文, 高橋 英樹, 川口 寧, 渡辺 登喜子, 松浦 善治, 鈴木 信弘, 長崎 慶三

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東京大学

    2016/06/30 - 2021/03/31

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    国際活動支援班では、各研究班の海外における研究活動を促進するために、 (1) 国際研究ネットワークの整備および強化、(2) 円滑な海外での研究試料の採取支援・提供、(3) 国際シンポジウムの開催および海外研究者の招聘による人的連携の促進、(4) サンプル収集のための海外への研究者の派遣の支援、(5) 最先端研究を行う先進諸国への若手研究者の中長期的派遣の支援、(6) 国際研究者ネットワークへの積極的な情報発信を行なっている。 2019年度は、国内で国際学会を4回 [Thermophiles国際学会、第18回あわじ感染と免疫国際フォーラム、日本ウイルス学会学術集会、Asian Mycological Congress 2019]、また、ワークショップを3回 [ウイルス情報解析ワークショップ、Thermophiles Workshop、ネオウイルス学ワークショップ]を共催して、海外から総計10名の研究者を招聘した。招聘した研究者と計画研究班、および公募研究班班員は学会後も共同研究を継続しており、人的連携を構築した。 また、南米のボリビア共和国に生息する節足動物の採集とウイルス調査を目的として、計画研究班の若手研究者を2名派遣して、サンプルの収集を実施させた。本活動には、計画研究班からさらに2名の研究者も参加して、蚊、ダニを採集した。また、本活動によるガブリエル・レネ・モレノ国立自治大学のJuan Antonio Pereira博士との共同研究に基づいて、計画研究班の若手研究者の研究室に所属する大学院生が、文部科学省の官民協働海外留学創出プロジェクト「トビタテ！留学JAPAN」を活用し、ガブリエル・レネ・モレノ国立自治大学に短期留学を実施し、現地での共同研究を推進した。 2018年度に引き続き、本領域のホームページの英語版を通して国際社会に本領域の活動を発信している。

  24. ヤドカリウイルスの新奇生活スタイル（宿借性）の証明

    鈴木 信弘, DAS SUBHA

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 岡山大学

    2018/04/25 - 2020/03/31

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    果樹白紋羽病菌のウイルスハンティングの過程で、ユニークなウイルス/ウイルス間相互作用を発見した。本菌から得られるウイルス精製画分からは、ヤドヌシウイルス(YnV1), ヤドカリウイルス(YkV1)が検出された。YnV1は非分節型dsRNAウイルスで、 キャプシド蛋白質(CP)とRNA合 成酵素(RdRp)をコードする。YkV1は RdRpドメインとプロテアーゼドメインをコードするプラス(+ )1本鎖RNAウイルスである。 具体的に下記の項目を実施し、「ヤドカリウイルスYkV1の宿借性]のコンセプトの確立に努めた。 1) 昨年作成したYkV1 RdRp GDDモティーフの変異株をさらに増やし、同様の解析を進め、「宿借性」にYkV1 RdRpが必須であることを確認した。 2) 昨年合成した６つの2A様プロテアーゼ変異株に加え、さらに６つの変異をYkV1感染性cDNA上で変異を導入し、感染性の有無とプロテアーゼ活性を調べた。その結果、バキュロウイルスを用いた昆虫細胞内でのプロテアーゼ活性と宿主菌でのウイルス複製能が一致した。すなわち、プロテアーゼ活性を消失した変異株は、白紋羽病菌での複製能も消失した。興味深いことに、弱いプロテアーゼ活性を有する変異株は、長期間維持すると2A様プロテアーゼが野生型に復帰したウイルス株が出現した。以上の結果は、YkV1の複製には、RdRp領域がポリプロテインから切断される必要があることを強く示唆する。 3) YkV1/YnV1感染株から塩化セシウム平衡密度勾配遠心法により、粒子を分画した結果、それぞれ単独で粒子化されていることを示唆するデータを得た。

  25. A new virus lifestyle: a ss(+)RNA virus hosted by a dsRNA virus

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows

    Category: Grant-in-Aid for JSPS Fellows

    Institution: Okayama University

    2015/11/09 - 2018/03/31

  26. Virome of the phytopathogenic fungi: metagenomic analysis and the possible utilization

    Kodno Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Okayama University

    2015/04/01 - 2018/03/31

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    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. However, virus discovery in obligate biotrophs such as powdery mildews (ascomycetes) and rust fungi (basidiomycetes) is still very limited. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least nine novel totivirus (genus Totivirus) in this fungus. Similar totvirus-like sequences are found in public transcriptome shotgun assembly (TSA) libraries of the bean rust fungi. Our data suggests that the horizontal transmission of ancestral totiviruses might have occurred across quite different fungal phyla, between powdery mildews and rust fungi and later evolved within the new host fungus separately.

  27. Exploration of infection mechanism and utilization of a mitochondrially replicating virus

    SUZUKI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Okayama University

    2015/04/01 - 2018/03/31

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    Mitoviruses are a unique group of mitochondrially replicating viruses with positive-sense, single stranded RNA genomes. Among them is Cryphonectria mitovirus 1 (CpMV1) which infects one of the three most destructive tree pathogens, chestnut blight fungus. This pathosystem involves two host/parasite interactions (chestnut vs. chestnut blight fungus) and chestnut blight fungus vs. CpMV1). This study represents the first example in which the host range, lateral transmissibility, symptom expression and counter-defense mechanism of a mitovirus was thoroughly analyzed.

  28. Antiviral innate immunity in phytopathogenic fungi

    SUZUKI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Okayama University

    2013/04/01 - 2017/03/31

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    The objective of this project is to explore RNA silencing (RNAi) as the antiviral innate defense against fungal viruses in plant pathogenic filamentous fungi. Consequently, a great progress has been made, leading to breakthroughs: 1) Discovery of RNAi mediated antiviral defense that requires Dicer but not AGO in Cryphonectria parasitica, 2) Detection of inhibitory effects on RNA silencing activities against RNA and DNA viruses, and transgenes in Pyricularia oryzae, 3) Development of a screening protocol for host factors required for virus perception through transcription induction of key genes of RNAi upon virus infection in C. parasitica, 4) Identification of the SAGA complex as a transcriptional regulator of fungal RNA silencing. These findings will bring about a paradigm shift in RNAi. Fungi are now established as the third position next to animals and plants in antiviral defense research. Note that most of the obtained data were published in international, high-quality journals.

  29. 白紋羽病菌のヴァイロコントロールに向けた新規２分節ｄｓＲＮＡの性状解析

    鈴木 信弘, JAMAL Atif

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 岡山大学

    2013/04/01 - 2015/03/31

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    白紋羽菌、Rosellinia necatrixは日本の果樹に重大な被害を齎している植物糸状菌病である。本研究では、白紋羽菌に感染し、その病原力を衰退させる新規ウイルス(Rosellinia necatrix megabirnavirus 1, RnMBV1)のウイルス学的性状とヴァイロコントロール（生物防除）因子としての潜在力を調べ、マイコウイルス学、ヴァイロコントールへの貢献を目指し、以下の成果を記述する。1-1ウイルス粒子トランスフェクション法を利用し、菌細胞質不和合性の複数の白紋羽病菌への感染性ならびに病原力低下効果を確認した。 1-2 RnMBV1を目が異なるクリ胴枯病菌への感染性ならびに病原力低下効果を確認した。RnMBV1のヴァイロコントロール因子としての可能性を広げる成果である(JAMAL氏が来日する以前に前倒しで実施し、論文掲載にこぎ着けた)。 1-3ヴァイロコントロール因子の長期安定保存が不可欠であるが、精製RnMBV1粒子は -80Cで１年以上安定に感染性を保持していた。 2-1 RnMBV1ゲノムセグメントはそれぞれ極めて長い(約1.6 kb)の非翻訳領域（ミニシストロンを複数含む）と２つのORFをもつ。5’側ORFがどのような機構で完全長mRNAから翻訳されるかを、リポーター系を確立した。 2-2 5’非翻訳領域がIRES（内部リボゾームエントリー部位）活性を持つことを確認し、さらにそのコア活性領域をORF1領域近傍の５００塩基の絞り込みに成功した。 2-3感染性RnMBV1核酸の合成を試みたが、成功には至らなかった。

  30. Endogenization process of non-retroviral RNA virus elements into plant genomes and their pathological significance

    KONDO Hideki, SUZUKI Nobuhiro, MARUYAMA Kazuyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Okayama University

    2012/04/01 - 2015/03/31

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    Endogenous non-retroviral RNA virus-like sequences (NRVSs) have been discovered in the genome of the vertebrates and other eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms. In this study, several novel NRVSs from the genome of the plants, insects and fungi were discovered. The presence of benyvirus-like sequences in the chickpea chromosomes is a second example of plant NRVSs related to positive-sense (+)ssRNA viruses. Benyvirus-related sequences were also found in the chromosomes of blood-sucking insect. In addition, the first (-)RNA virus infection in fungus was evidenced based on a discovery of mononegavirus L-like sequences in the genome of the powdery mildew fungus. Our findings may provide novel insights into the origin and evolution of ssRNA viruses. The possible endogenization process of these NRVSs into the genome and its biological significance on the host were discussed.

  31. Development of Neurospora crassa as a model filamentous fungus for studying virus/host interactions.

    SUZUKI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Okayama University

    2012/04/01 - 2015/03/31

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    As a model system for genetics, biochemistry or developmental biology, Neurospora crassa has greater advantages over any other filamentous fungi due to its even short life cycle time, ease of crossing, and well-established tools and resources represented by a number of gene knockout strains available from the Fungal Genetics Stock Center. This study aimed at screening of previously reported fungal viruses for N. crassa-infectious viruses. We confirmed that N. crassa supports the replication of two heterologous viruses, mycoreovirus 1 (MyRV1) and Rosellinia necatrix partitivirus 2 (RnPV2) from other ascomycetes, Cryphonectrial parasitica and R. necatrix. The two viruses could be maintained not only in the wild-type strain but also RNA silencing defective mutants of N. crassa. It is now being investigated what effects of deletions of RNA silencing-related genes on virus replication are, and how these genes respond to virus infection.

  32. ハイポウイルスの多機能性蛋白質ｐ２９により誘導されるレオウイルスゲノム再編成

    鈴木 信弘, COPE A.E.

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 岡山大学

    2011 - 2013

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    クリ胴枯病菌(Cryphonectria parasitica)は子のう菌の一種でクリ樹を枯死させる植物病原菌である。クリ胴枯病菌は少なくとも5科のメンバーの宿主となっている。本研究では、異種ウイルス(ハイポウイルス)の蛋白質(p29)で誘発可能なレオウイルス(MyRV1)ゲノムセグメントの遺伝子再編成を多方向から解析した。今年度は上記目的のため以下の3項目を実施した(申請書に記載した実施項目3,4の実施状況については昨年度のC-7-1を参照されたい)。 5p29のmiRNAへの影響 糸状菌ではsiRNAは同定されているが、miRNAは未だ発見されていない。そこで、miRNAのクローニングを試みた。しかし、それらの同定には至らなかった。但し、これを相殺する成果として、アカパンカビあるいは植物でsiRNAの増幅に関与すると考えられるRNA依存RNA合成酵素遺伝子(rdr1)の破壊株を得るのに成功し、破壊株では再編成誘導が起らないことを明らかにすることに成功した。 6植物ウイルス由来RNAサイレンシング抑制蛋自質の遣伝子属編成誘導能 RNAサイレンシング抑制能と遺伝子再編成誘導能との関連が示された場合は、他のRNAサイレンシング抑制蛋白質(例えば、植物ウイルス由来のPotyvirus HC-Pro, cucumovirus 2b, tombusvirus p19)のMyRV1遺伝子再編成誘導能を検定した。しかし、結果は陰性であった。

  33. Studies on the mechanism of viroplasm formation during plant negative-strand RNA virus infection

    KONDO Hideki, SUZUKI Nobuhiro, MARUYAMA Kazuyuki, IDA Bagus andika

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Okayama University

    2009 - 2011

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    Orchid fleck virus(OFV), a unique two-segmented negative-sense RNA virus, induces an intranuclear electron-lucent viroplasm(inclusion) in infected plant cells. We studied the molecular mechanism by which OFV viroplasms are produced in vivo. Transiently expression examinations by Agrobacterium-infiltration reveled that coexpression of nucleocapsid protein N and the putative phosphoprotein P, in the absence of virus infection, was sufficient for the formation of an intranuclear electron-lucent viroplasm-like structure in Nicotiana benthamiana cells.

  34. 根におけるRNAサイレンシング調節機構の解明とウイルス抵抗性植物創成への応用

    鈴木 信弘, ANDIKA I.

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 岡山大学

    2007 - 2008

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    本研究では,「根でのRNAサイレンシング活性に関与する宿主因子とそれを低減する因子」を解析した.まず,第一にトランスジーンやウイルスに対するサイレンシング活性が根で低いという現象の一般化を検討した.すなわち,数種のウイルスと宿主植物の組み合わせで調べ,根におけるウイルスのサイレンシング活性抑制効果についても詳細に解析した.次に,トランスジーン,ウイルス由来のsiRKAの根での蓄積量が低かったことから,その産生に関与するDCL群などの宿主因子を中心に,アラビドプシスの葉と根での発現パターンを解析した.さらに,候補となる宿主因子の変異体について,トランスジーンやウイルス等に対する影響を評価した. 1.[根でのRNAサイレンシングにより影響される複製レベルはウィルスにより異なる] トランスジーンに対するサイレンシングが根で低いことは植物種を越えて一般化できるが,ウイルスに対するサイレング効果はウイルス種により異なっていた. 2.[RNAサイレンシング関連遺伝子の変異体とウイルス感受性] PVXをアラビドプシス(非宿主とされていた)のdcl変異体へ接種したところ,DCL2とDCL4の変異体で効率的に全身移行したが,根ではDCL2,DCL4に加えDCL3もPVXの蓄積に影響することが示唆された. 3.[サイレンシング関連遺伝子変異体における内在型siRNAの蓄積とトランスジーンサイレンシングへの影響] 現在実施中. 4.[サイレンシング活性低減要因(遺伝子)の根における強制的発現ラインの作出] 活性の低減に関わる候補遺伝子BNYVV p14をN.benthamiana根で強制的に発現させた.

  35. Genome transport mechanisms of plant negative-stranded RNA virus

    KONDO Hideki, SUZUKI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Okayama University

    2007 - 2008

  36. Analysis of RNA silencing suppressor activities of viral proteins in a chestnut blight fungus system

    SUZUKI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: OKAYAMA UNIVERSITY

    2002 - 2004

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    Post transcriptional gene silencing(PTGS), first discovered in plants independently as cosuppresion and RNA-mediated virus resistance, is a sequence-specific RNA degradation system which is now believed to be ubiquitous in eukaryotic organisms from fungi to mammals. Double-stranded RNA is key molecules triggering PTGS and being digested by RNaseIII type nuclease to 22-25 nucleotides (siRNA, a hall mark of PTGS) which guide RISC (RNA inducing silencing complex, AGO family member being a major component) to target RNA and lead to the degradation of the RNA. PTGS is conceptualized as an anti-virus host defense mechanism by several lines of evidence. As its counterattack strategy plant and animal viruses as well encode PTGS suppressors which seem to act in different ways. Most of them are derived from positive and negative strand RNA or DNA viruses. Here in this project dsRNA viruses were subjected to anti-PTGS activity assay in a filamentous fungus, Cryphonectria parasitica (the chestnut blight fungus) and animal. Proteins tested included those encoded by the prototype hypovirus, CHV1-EP713, two plant reoviruses, dicot plant-infecting wound tumor virus(WTV) and monocot plant-infecting rice dwarf virus(RDV), the latter two of witch are also insect (animal)-infecting. 1)Some RNA silencing suppressors are known to enhance replication of heterologous viruses. Here suppressive activities of candidate proteins of different virus origins were evaluated based on the levels of MYRV1, a member of a newly established genus within the family Reoviridae. By this assay the papain-like proteinase p29 derived from the prototype hypovirus (CHV1), was shown to elevate the replication and vertical transmission levels of MYRV1, indicative the RNA silencing suppressive activity of p29, while no such activity by p40 of CHV1, reported to augment the homologous virus replication, was not found. Viral proteins encoded by plant- and insect-infecting reoviruses, i.e., rice dwarf phytoreovirus and wound tumor virus were tested. However, no elevation of MYRV1 replication was found by those proteins. 2)Trangenic C parasitica lines silenced for Hygromycin resistance gene(HygR) were attempted to be generated. To this end, C parasitica spheroplasts were transformed with HygR under the control of constitutive promoter. Resulting transgenic lines were doubly transformed with an inverted repeat of HygR with an intron spacer. Out of more than 200 independent transformants, approximately 10-20 showed Hyg succeptible, probably because of RNA silecing triggered by the dsRNA form of HygR. These isolates were being used for RNA silencing suppressor assay in which the candidate proteins as above are examined for the ability to reverting their phenotype to HygR.

  37. イネ萎縮ウイルス複製酵素複合体の再構成

    鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 秋田県立農業短期大学

    1995 - 1995

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    イネ萎縮ウイルスはファイトレオウイルスんい属するウイルスで、12本の文節2本鎖RNAをゲノムに持つ。昨年全ゲノムの構造解析が完了し(植物レオウイルスとしては最初である)、各ゲノムが4塩基の末端保存配列、逆反復配列、単一のORFから構成されることが示された。末端保存配列、逆反復配列の末端配列ドメインはゲノムセグメントの粒子への詰め込みと複製・転写に関与することが示唆されているが、明らかとなっていない。本研究では末端保存配列と相互作用するであろうウイルス蛋白質、宿主蛋白質の同定、さらにそれら蛋白質と相互作用するシス配列の同定を最終目的に、RNA合成活性を持つRDVコア粒子の再構成を試みた。 RDVのコア粒子を構成する蛋白質遺伝子全て(S1、S3、S5 S7)を昆虫(夜盗蛾)細胞、Spodoptera frugiperdaで発現させることに成功した。さらに疑似コア粒子と考えられる構造物の部分精製も行った。但し、現在までのところ、この構造物のRNA合成活性は確認されていない。今後、コア粒子様構造物の精製法、鋳型導入法等の検討を要する。 研究過程でRDV複製に関与するであろう酵素について興味深い発見をした。以下に記す。これまで、P2、P5はアウタ-レイヤー蛋白質と考えられていたが、昨年度の奨励研究Aによりマイナ-コア蛋白質をコードすることが明らかとなった。本研究によりこのP5がGTPと共有結合することが示された。P5はmRNA転写の際のキャッピング酵素、すなわちグアニル酸転移酵素あるることが示唆された。また、P5はGTPのほかにATP,UTPとの結合活性を持っていた。この活性はこれまで報告されているグアニル酸転移酵素とは大きく異なる性質である。ATP,UTP,GTPの結合サイトは同じか?、ATP,UTP結合能の生物学的意義は何か?等、今後の大きな検討課題である。

  38. イネ萎縮ウイルスのヨコバイ細胞感染性を支配するウイルス因子の同定

    鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 秋田県立農業短期大学

    1994 - 1994

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    1)他の植物レオウイルスで得られた過去の結果と予備実験の結果から、S2、S5がウイルス粒子表面に存在する蛋白質(P2、P5)をコードすること、さらにこれらがウイルス精製過程で脱落するすることが示唆された。まず、これをP2、P5に対する抗体を用いて証明した。 RDV S2(一部)、S5(完全長)のcDNAをそれぞれ夜盗蛾培養細胞中で発現させた。P2のカルボキシル基末端半分(60 kDa)と89kDaのP5の大量発現が確認された。これらのRDV由来の蛋白質をウサギに免疫し、抗血清を得た。免疫電顕法を用いてこれらの抗体と粒子との反応性を調べた結果、P2血清は粒子と反応したが、P5血清は反応しなかった。次にウエスタン法を用いて精製粒子、精製コア(内殻)粒子との反応性を調べた。P2血清は精製粒子中の130 kD蛋白質と反応したが、コア粒子中には反応する蛋白質は検出されなかった。一方、P5血清は精製粒子中の89kDマイナ-蛋白質と反応した。また、この蛋白質はP5抗体によってコア粒子からも微量検出された。 これらの結果から、P2は予想された通り粒子の外殻粒子に存在するがP5については外殻蛋白質ではなく内殻粒子を構成する微量成分であることが明らかになった。S5については従来S2と同様に外殻蛋白質をコードすると考えられてきた。しかし、興味深いことにコア蛋白質をコードすることが証明された。植物レオウイルスの中で遺伝子-産物の全ての対応が明らかとなったのはRDVが初めてであり、世界的に評価されうる大きな成果である。 従って、P2とこれまでに外殻蛋白質と同定されているP8がヨコバイ培養細胞感染性を支配することが示唆された。 2)ヨコバイ培養細胞株の樹立。 木村の方法(1987年)によってツマグロヨコバイ卵細胞の株化を試みた。初代培養には成功したが、安定継代株はえられていない。 今後、安定なヨコバイ培養細胞株を樹立し、P2・P8抗体を用いたRDVの感染中和試験を行う必要がある。また、RDVS2コードのP2を完全な状態で組み換えバキュロウイルス発現夜盗蛾細胞から精製し、その精製P2を用いて完全粒子の再構成系を確立する必要がある。

  39. 虫媒伝染性欠損に関与するイネ萎縮ウイルスの同定

    鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 秋田県立農業短期大学

    1993 - 1993

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    (1)イネ萎縮ウイルス(RDV)はヨコバイで永続的に媒介され、しかも虫体内で増殖し高率に経卵伝染する。しかし、本ウイルスを数年イネ植物体だけで継代することによって(感染イネを株分けで栽培し続ける)、伝染性が失われるという興味深い現象が見いだされる。本研究ではまず、この伝染性が欠落したRDV感染イネを得た。 (2)野性型とヨコバイ伝染性欠損型RDVでゲノムセグメントの電気泳動像に違いがあるかどうかまず調べた結果、両者に差は認められなかった。 (3)RDVゲノムセグメントがコードする蛋白質をバキュロウイルスベクターを用いて発現させ、それらに対する抗体を作製した。 (4)3)で作製した抗体を用いて野性型、伝染性欠損型感染イネ中のRDV蛋白質の検出を行なった。イネ個体間で検出量に差は認められたが、野性型と欠損型との間では明確な違いは認められなかった。 これらの結果は、他のレオウイルスで報告されているような特定セグメントの欠失によるのではなく、他のメカニズム(例えば点突然変異)によってRDVのヨコバイ伝染性欠損が起こることを示唆する。

  40. イネのロイシンジッパ-型DNA結合タンパク質の遺伝子構造と機能

    草野 友延, 鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 重点領域研究

    Category: 重点領域研究

    Institution: 秋田県立農業短期大学

    1991 - 1993

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    我々は,種々の環境ストレスのうち低温(5℃)がイネ植物に及ぼす影響を遺伝子発現のレベルの違いで検出しようと研究を進めてきた。独自に開発したcDNAライブラリ-作成法及びサブトラクション法〔Nucleic Acids Res.18:1071(1990),Plaut Cell Physiol.32:in press(1991)〕により低温処理で特異的に誘導される3種のイネcDNAクロ-ンの分離に成功してきた。これら3種のクロ-ンは、低温処理との関連が示唆されているアブシジン酸の処理あるいは高浸透圧(0.5Mマニト-ル)処理によっては誘導はおこらず,また熱ショク処理によっても誘導されない。更には1度低温で誘導されたものを常温25℃に戻してやると転写レベルでの誘導が全くおこっていない事も確認している。従ってこれらのクロ-ンは低温処理により特異的に発現が誘導されると結論した。 これらのクロ-ンのうちLip19と命名したものは1323塩基対からなり,1次構造解析及びセンス鎖の同定などより約19,000の分子量のタンパク質をコ-ドし,その遺伝子産物はタンパク質デ-タベ-ス検索の結果トウモロコシ由来のジッパ-モチ-フをもつDNA結合タンパク質であることが明らかになっている。 ゲノミックサザン法によりlip19遺伝子は1コピ-であることが判明した。λgtloの部分ライブラリ-より,ゲノミッククロ-ンを4kbのEcoRI断片として単離した。物理地図及び1次構造の解析により,lip19は翻訳領域,非翻訳領域を問わず挿入配列をもたないことが明らかとなった。現在,E.ColiのTクポリメラ-ゼ/Tクプロモ-タ-発現系で作らせたLip19を用い,DNA認識配列の同定を行っている。

  41. ライボザイムを利用したイネ委縮病の防除

    鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 秋田県立農業短期大学

    1992 - 1992

  42. イネ委縮ウイルスmRNAのイネおよび昆虫培養細胞への導入

    鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 秋田県立農業短期大学

    1990 - 1990

  43. イネ萎縮ウイルスの遺伝的解析

    鈴木 信弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 秋田県立農業短期大学

    1989 - 1989

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Teaching Experience 15

  1. ウイルス学（分担、オムニバス）　岡山大学医学部

  2. 発展ウイルス学

  3. ウイルス分子生物学演習

  4. Advances in Plant Stress Science　Okayama University Grad School for Environemntal and Life Science

  5. 植物－微生物／細菌相互作用　岡山大学環境生命科学研究科

  6. 植物ウイルス学　岡山大学農学部

  7. Food and Environment Issues　Graduate School of Environmental and Life Science

  8. ASEAN人材育成特別プログラム（オムニバス）　岡山大学環境生命研究科

  9. 環境生命研究科専攻概論（オムニバス）　岡山大学環境生命科学研究科

  10. 植物－微生物／昆虫相互作用　岡山大学環境生命科学研究科

  11. 第13回 生産・環境生物学特別講義 「菌類ウイルス学への招待」　東京大学農学部

  12. 第13回 生産・環境生物学特別講義「ウイルスいろいろ：役立つウイルス、目立たないウイルス」　東京大学農学部

  13. 教養特別講義

  14. 微生物・ウイルス機能解析学　岡山大学自然科学研究科

  15. 植物医科学入門　岡山大学 一般教養科目（３コマ）

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Social Activities 15

  1. 今だから聞けるウイルス学講座「菌類ウイルスに魅せられて」

    湯河原ウイルスキャンプ

    2018/06/06 - 2018/06/07

  2. 岡山大学先端研究講座 ネオウイルス学:ウイルスと生きる

    岡山大学先端研究講座

    2017/06/17 - 2017/06/17

  3. これでもウイルス？

    みちのくウイルス塾

    2016/07/16 - 2016/07/17

  4. 職場体験

    2013/10/16 - 2013/10/19

  5. ウイルスいろいろ：目立たないウイルス、植物の守り神となるウイルス

    みちのくウイルス塾

    2012/07/14 - 2012/07/15

  6. ヴァイロコントロール：悪玉カビを病気にする善玉ウイルス

    ウイルス学キャンプ

    2010/08/09 - 2010/08/10

  7. ミクロの世界へようこそ、ウイルス学入門

    Summer Science School SSS2021

    2021/08/02 -

  8. ウイルスと生きる：SARS-CoV-2.

    Summer Science School online 2020

    2020/11/21 -

  9. 研究室紹介

    高校生研究所訪問

    2017/03/14 -

  10. ウイルスの有効利用〜ウイルスで病気を制す：「ヴァイロコントール」〜

    2016/11/11 -

  11. 日本学術振興会（JSPS）サイエンス・ダイアログ プログラム

    2013/02/14 -

  12. マイコウイルスとヴァイロコントロール

    平成24年度植物感染生理談話会

    2012/08/30 -

  13. ウイルスいろいろ：役立つウイルス、目立たないウイルス

    平成24年度 公開講座「ウイルス -摩訶不思議な不完全生命体-」

    2012/05/26 -

  14. 病害虫防除の最前線」植物の病気の生物防除=ウイルスをもってカビを制す=

    吉備創生カレッジ講座

    2009/06/27 -

  15. 賢いウイルスとその遺伝子

    岡山大学公開講座

    2002/07 -

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