Details of the Researcher

PHOTO

Nobuharu Fujii
Section
Graduate School of Life Sciences
Job title
Associate Professor
Degree

  • Ph.D. (The University of Tokyo)

Research History 3

  • 1999/05 - 2000/05
    Department of Biochemistry, University of Missouri, USA　研究員

  • 1999/01 - 1999/03
    Department of Biochemistry, University of Missouri, USA　研究員

  • 1992/04 - 1995/03
    Fellow of the Japan Society for the Science for Japanese Junior Scientists.

Education 3

  • The University of Tokyo　Graduate School, Division of Science　植物学専攻

    - 1995/03

  • University of Tsukuba　Graduate School, Division of Environmental Science

    - 1992/03

  • University of Tsukuba　Second Cluster of College　生物学類

    - 1989/03

Committee Memberships 2

  • 東北植物学会　会計幹事

    2020 - Present

  • 宇宙生物科学会　副編集委員

    2009/04 - Present

Professional Memberships 5

  • Japanese Society for Plant Cell and Molecular Biology

  • The Molecular Biology Society of Japan

  • The Botanical Society of Japan

  • The Japanese Society of Plant Physiologists

  • Japanese Society for Biological Sciennces in Space

Research Interests 3

  • 植物ホルモン

  • 環境

  • 植物

Research Areas 2

  • Life sciences / Applied molecular and cellular biology /

  • Life sciences / Plants: molecular biology and physiology /

Papers 69

  1. Heterotic growth of hybrids of Arabidopsis thaliana is enhanced by elevated atmospheric CO2

    Masako Mishio, Emi Sudo, Hiroshi Ozaki, Riichi Oguchi, Ryo Fujimoto, Nobuharu Fujii, Kouki Hikosaka

    American Journal of Botany　2024/04

    DOI： 10.1002/ajb2.16317 　

  2. MIZU-KUSSEI1 (MIZ1) and GNOM/MIZ2 control not only positive hydrotropism but also phototropism in Arabidopsis roots

    Lei Pang, Akie Kobayashi, Yuka Atsumi, Yutaka Miyazawa, Nobuharu Fujii, Daniela Dietrich, Malcolm J Bennett, Hideyuki Takahashi

    Journal of Experimental Botany　2023/05/23

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/jxb/erad193 　

    ISSN： 0022-0957

    eISSN： 1460-2431

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    Abstract In response to unilateral blue light illumination, roots of some plant species such as Arabidopsis thaliana exhibit negative phototropism (bending away from light), which is important for light avoidance in nature. MIZU-KUSSEI1 (MIZ1) and GNOM/MIZ2 are essential for positive hydrotropism (i.e. in the presence of a moisture gradient, root bending towards greater water availability). Intriguingly, mutations in these genes also cause a substantial reduction in phototropism. Here, we examined whether the same tissue-specific sites of expression required for MIZ1- and GNOM/MIZ2-regulated hydrotropism in Arabidopsis roots are also required for phototropism. The attenuated phototropic response of miz1 roots was completely restored when a functional MIZ1–green fluorescent protein (GFP) fusion was expressed in the cortex of the root elongation zone but not in other tissues such as root cap, meristem, epidermis, or endodermis. The hydrotropic defect and reduced phototropism of miz2 roots were restored by GNOM/MIZ2 expression in either the epidermis, cortex, or stele, but not in the root cap or endodermis. Thus, the sites in root tissues that are involved in the regulation of MIZ1- and GNOM/MIZ2-dependent hydrotropism also regulate phototropism. These results suggest that MIZ1- and GNOM/MIZ2-mediated pathways are, at least in part, shared by hydrotropic and phototropic responses in Arabidopsis roots.

  3. Diversity of root hydrotropism among natural variants of Arabidopsis thaliana

    Boyuan Mao, Hiroki Takahashi, Hideyuki Takahashi, Nobuharu Fujii

    Journal of Plant Research　135　(6)　799-808　2022/09/23

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10265-022-01412-w 　

    ISSN： 0918-9440

    eISSN： 1618-0860

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    Abstract Root gravitropism affects root hydrotropism. The interference intensity of root gravitropism with root hydrotropism differs among plant species. However, these differences have not been well compared within a single plant species. In this study, we compared root hydrotropism in various natural variants of Arabidopsis under stationary conditions. As a result, we detected a range of root hydrotropism under stationary conditions among natural Arabidopsis variants. Comparison of root gravitropism and root hydrotropism among several Arabidopsis natural variants classified natural variants that decreased root hydrotropism into two types; namely one type that expresses root gravitropism and root hydrotropism weaker than Col-0, and the other type that expresses weaker root hydrotropism than Col-0 but expresses similar root gravitropism with Col-0. However, root hydrotropism of all examined Arabidopsis natural variants was facilitated by clinorotation. These results suggested that the interference of root gravitropism with root hydrotropism is conserved among Arabidopsis natural variants, although the intensity of root gravitropism interference with root hydrotropism differs.

  4. Circumnutational movement in rice coleoptiles involves the gravitropic response: analysis of an agravitropic mutant and space-grown seedlings. Peer-reviewed

    Kobayashi A, Kim HJ, Tomita Y, Miyazawa Y, Fujii N, Yano S, Yamazaki C, Kamada M, Kasahara H, Miyabayashi S, Shimazu T, Fusejima Y, Takahashi H

    Physiologia plantarum　2018/08

    DOI： 10.1111/ppl.12824 　

    ISSN： 0031-9317

  5. Root-tip-mediated inhibition of hydrotropism is accompanied with the suppression of asymmetric expression of auxin-inducible genes in response to moisture gradients in cucumber roots Peer-reviewed

    Nobuharu Fujii, Sachiko Miyabayashi, Tomoki Sugita, Akie Kobayashi, Chiaki Yamazaki, Yutaka Miyazawa, Motoshi Kamada, Haruo Kasahara, Ikuko Osada, Toru Shimazu, Yasuo Fusejima, Akira Higashibata, Takashi Yamazaki, Noriaki Ishioka, Hideyuki Takahashi

    PLoS ONE　13　(1)　e0189827　2018/01/01

    Publisher: Public Library of Science

    DOI： 10.1371/journal.pone.0189827 　

    ISSN： 1932-6203

  6. Gravitropism interferes with hydrotropism via counteracting auxin dynamics in cucumber roots: clinorotation and spaceflight experiments Peer-reviewed

    Keita Morohashi, Miki Okamoto, Chiaki Yamazaki, Nobuharu Fujii, Yutaka Miyazawa, Motoshi Kamada, Haruo Kasahara, Ikuko Osada, Toru Shimazu, Yasuo Fusejima, Akira Higashibata, Takashi Yamazaki, Noriaki Ishioka, Akie Kobayashi, Hideyuki Takahashi

    NEW PHYTOLOGIST　215　(4)　1476-1489　2017/09

    DOI： 10.1111/nph.14689 　

    ISSN： 0028-646X

    eISSN： 1469-8137

  7. Mutant selection in the self-incompatible plant radish (Raphanus sativus L. var. sativus) using two-step TILLING Peer-reviewed

    Kaori Kohzuma, Motoko Chiba, Soichiro Nagano, Toyoaki Anai, Miki U. Ueda, Riichi Oguchi, Kazumasa Shirai, Kousuke Hanada, Kouki Hikosaka, Nobuharu Fujii

    BREEDING SCIENCE　67　(3)　268-276　2017/06

    DOI： 10.1270/jsbbs.16200 　

    ISSN： 1344-7610

    eISSN： 1347-3735

  8. Auxin transport and response requirements for root hydrotropism differ between plant species Peer-reviewed

    Yusuke Nakajima, Yoshitaka Nara, Akie Kobayashi, Tomoki Sugita, Yutaka Miyazawa, Nobuharu Fujii, Hideyuki Takahashi

    JOURNAL OF EXPERIMENTAL BOTANY　68　(13)　3441-3456　2017/06

    DOI： 10.1093/jxb/erx193 　

    ISSN： 0022-0957

    eISSN： 1460-2431

  9. Root hydrotropism is controlled via a cortex-specific growth mechanism Peer-reviewed

    Daniela Dietrich, Lei Pang, Akie Kobayashi, John A. Fozard, Veronique Boudolf, Rahul Bhosale, Regina Antoni, Tuan Nguyen, Sotaro Hiratsuka, Nobuharu Fujii, Yutaka Miyazawa, Tae-Woong Bae, Darren M. Wells, Markus R. Owen, Leah R. Band, Rosemary J. Dyson, Oliver E. Jensen, John R. King, Saoirse R. Tracy, Craig J. Sturrock, Sacha J. Mooney, Jeremy A. Roberts, Rishikesh P. Bhalerao, Jose R. Dinneny, Pedro L. Rodriguez, Akira Nagatani, Yoichiroh Hosokawa, Tobias I. Baskin, Tony P. Pridmore, Lieven De Veylder, Hideyuki Takahashi, Malcolm J. Bennett

    NATURE PLANTS　3　(6)　17057　2017/06

    DOI： 10.1038/nplants.2017.57 　

    ISSN： 2055-026X

    eISSN： 2055-0278

  10. Root hydrotropism is controlled via a cortex-specific growth mechanism Peer-reviewed

    Daniela DIetrich, Lei Pang, Akie Kobayashi, John A. Fozard, Véronique Boudolf, Rahul Bhosale, Regina Antoni, Tuan Nguyen, Sotaro Hiratsuka, Nobuharu Fujii, Yutaka Miyazawa, Tae-Woong Bae, Darren M. Wells, Markus R. Owen, Leah R. Band, Rosemary J. Dyson, Oliver E. Jensen, John R. King, Saoirse R. Tracy, Craig J. Sturrock, Sacha J. Mooney, Jeremy A. Roberts, Rishikesh P. Bhalerao, José R. DInneny, Pedro L. Rodriguez, Akira Nagatani, Yoichiroh Hosokawa, Tobias I. Baskin, Tony P. Pridmore, Lieven De Veylder, Hideyuki Takahashi, Malcolm J. Bennett

    Nature Plants　3　2017/05/08

    Publisher: Palgrave Macmillan Ltd.

    DOI： 10.1038/nplants.2017.57 　

    ISSN： 2055-0278

  11. The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy Peer-reviewed

    Chiaki Yamazaki, Nobuharu Fujii, Yutaka Miyazawa, Motoshi Kamada, Haruo Kasahara, Ikuko Osada, Toru Shimazu, Yasuo Fusejima, Akira Higashibata, Takashi Yamazaki, Noriaki Ishioka, Hideyuki Takahashi

    NPJ MICROGRAVITY　2　16030　2016/09

    DOI： 10.1038/npjmgrav.2016.30 　

    ISSN： 2373-8065

  12. Gravitropic response and circumnutation in pea (Pisum sativum) seedling roots Peer-reviewed

    Hye-jeong Kim, Akie Kobayashi, Nobuharu Fujii, Yutaka Miyazawa, Hideyuki Takahashi

    PHYSIOLOGIA PLANTARUM　157　(1)　108-118　2016/05

    DOI： 10.1111/ppl.12406 　

    ISSN： 0031-9317

    eISSN： 1399-3054

  13. GNOM regulates root hydrotropism and phototropism independently of PIN-mediated auxin transport Peer-reviewed

    Teppei Moriwaki, Yutaka Miyazawa, Nobuharu Fujii, Hideyuki Takahashi

    PLANT SCIENCE　215　141-149　2014/02

    DOI： 10.1016/j.plantsci.2013.11.002 　

    ISSN： 0168-9452

  14. MIZ1-regulated hydrotropism functions in the growth and survival of Arabidopsis thaliana under natural conditions Peer-reviewed

    Satoru Iwata, Yutaka Miyazawa, Nobuharu Fujii, Hideyuki Takahashi

    Annals of Botany　112　(1)　103-114　2013/07

    DOI： 10.1093/aob/mct098 　

    ISSN： 0305-7364 1095-8290

  15. Overexpression of MIZU-KUSSEI1 enhances the root hydrotropic response by retaining cell viability under hydrostimulated conditions in arabidopsis thaliana Peer-reviewed

    Yutaka Miyazawa, Teppei Moriwaki, Mayumi Uchida, Akie Kobayashi, Nobuharu Fujii, Hideyuki Takahashi

    Plant and Cell Physiology　53　(11)　1926-1933　2012/11

    DOI： 10.1093/pcp/pcs129 　

    ISSN： 0032-0781 1471-9053

  16. Light and abscisic acid signalling are integrated by MIZ1 gene expression and regulate hydrotropic response in roots of Arabidopsis thaliana Peer-reviewed

    Teppei Moriwaki, Yutaka Miyazawa, Nobuharu Fujii, Hideyuki Takahashi

    Plant, Cell and Environment　35　(8)　1359-1368　2012/08

    DOI： 10.1111/j.1365-3040.2012.02493.x 　

    ISSN： 0140-7791 1365-3040

  17. MIZ1, an essential protein for root hydrotropism, is associated with the cytoplasmic face of the endoplasmic reticulum membrane in Arabidopsis root cells Peer-reviewed

    Tomokazu Yamazaki, Yutaka Miyazawa, Akie Kobayashi, Teppei Moriwaki, Nobuharu Fujii, Hideyuki Takahashi

    FEBS Letters　586　(4)　398-402　2012/02/17

    DOI： 10.1016/j.febslet.2012.01.008 　

    ISSN： 0014-5793 1873-3468

  18. A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots Peer-reviewed

    Mayumi Nakayama, Yasuko Kaneko, Yutaka Miyazawa, Nobuharu Fujii, Nahoko Higashitani, Shinya Wada, Hiroyuki Ishida, Kohki Yoshimoto, Ken Shirasu, Kenji Yamada, Mikio Nishimura, Hideyuki Takahashi

    Planta　236　(4)　999-1012　2012

    DOI： 10.1007/s00425-012-1655-5 　

    ISSN： 0032-0935 1432-2048

  19. Gravistimulation Changes the Accumulation Pattern of the CsPIN1 Auxin Efflux Facilitator in the Endodermis of the Transition Zone in Cucumber Seedlings Peer-reviewed

    Chiaki Watanabe, Nobuharu Fujii, Kenichi Yanai, Takuya Hotta, Dai-Hee Kim, Motoshi Kamada, Yuko Sasagawa-Saito, Takeshi Nishimura, Tomokazu Koshiba, Yutaka Miyazawa, Kyung-Min Kim, Hideyuki Takahashi

    PLANT PHYSIOLOGY　158　(1)　239-251　2012/01

    DOI： 10.1104/pp.111.188615 　

    ISSN： 0032-0889

  20. Hormonal regulation of lateral root development in Arabidopsis modulated by MIZ1 and requirement of GNOM activity for MIZ1 function Peer-reviewed

    Teppei Moriwaki, Yutaka Miyazawa, Akie Kobayashi, Mayumi Uchida, Chiaki Watanabe, Nobuharu Fujii, Hideyuki Takahashi

    Plant Physiology　157　(3)　1209-1220　2011/11

    DOI： 10.1104/pp.111.186270 　

    ISSN： 0032-0889 1532-2548

  21. Factors responsible for deep-sowing tolerance in wheat seedlings: Varietal differences in cell proliferation and the co-ordinated synchronization of epidermal cell expansion and cortical cell division for the gibberellin-mediated elongation of first internodes Peer-reviewed

    Fumie Kato, Masaru Araki, Yutaka Miyazawa, Nobuharu Fujii, Kazuyoshi Takeda, Hiroshi Suge, Hideyuki Takahashi

    Annals of Botany　108　(3)　439-447　2011/09

    DOI： 10.1093/aob/mcr173 　

    ISSN： 0305-7364 1095-8290

  22. Expression analysis of the auxin efflux carrier family in tomato fruit development Peer-reviewed

    Sogo Nishio, Ryo Moriguchi, Hiroki Ikeda, Hideki Takahashi, Hideyuki Takahashi, Nobuharu Fujii, Thomas J. Guilfoyle, Koki Kanahama, Yoshinori Kanayama

    PLANTA　232　(3)　755-764　2010/08

    DOI： 10.1007/s00425-010-1211-0 　

    ISSN： 0032-0935

  23. Differential accumulation of CsARF5 protein during peg formation in cucumber seedlings Peer-reviewed

    Sasagawa-Saito Y, Kitazawa D, Miyazawa Y, Fujii N, Takahashi H

    Biological Sciences in Space　24　153-162　2010

  24. A molecular mechanism unique to hydrotropism in roots Invited

    Yutaka Miyazawa, Yoshie Ito, Teppei Moriwaki, Akie Kobayashi, Nobuharu Fujii, Hideyuki Takahashi

    PLANT SCIENCE　177　(4)　297-301　2009/10

    DOI： 10.1016/j.plantsci.2009.06.009 　

    ISSN： 0168-9452

  25. Hormonal interactions during root tropic growth: hydrotropism versus gravitropism Invited Peer-reviewed

    Hideyuki Takahashi, Yutaka Miyazawa, Nobuharu Fujii

    PLANT MOLECULAR BIOLOGY　69　(4)　489-502　2009/03

    DOI： 10.1007/s11103-008-9438-x 　

    ISSN： 0167-4412

    eISSN： 1573-5028

  26. GNOM-Mediated Vesicular Trafficking Plays an Essential Role in Hydrotropism of Arabidopsis Roots Peer-reviewed

    Yutaka Miyazawa, Akiko Takahashi, Akie Kobayashi, Tomoko Kaneyasu, Nobuharu Fujii, Hideyuki Takahashi

    PLANT PHYSIOLOGY　149　(2)　835-840　2009/02

    DOI： 10.1104/pp.108.131003 　

    ISSN： 0032-0889

  27. Characterization of a novel gravitropic mutant of morning glory, weeping2 Peer-reviewed

    Daisuke Kitazawa, Yutaka Miyazawa, Nobuharu Fujii, Eiji Nitasaka, Hideyuki Takahashi

    ADVANCES IN SPACE RESEARCH　42　(6)　1050-1059　2008/09

    DOI： 10.1016/j.asr.2007.10.029 　

    ISSN： 0273-1177

  28. Effects of locally targeted heavy-ion and laser microbeam on root hydrotropism in Arabidopsis thaliana Peer-reviewed

    Yutaka Miyazawa, Tetsuya Sakashita, Tomoo Funayama, Nobuyuki Hamada, Hiroshi Negishi, Akie Kobayashi, Tomoko Kaneyasu, Atsushi Ooba, Keita Morohashi, Takehiko Kakizaki, Seiichi Wada, Yasuhiko Kobayashi, Nobuharu Fujii, Hideyuki Takahashi

    JOURNAL OF RADIATION RESEARCH　49　(4)　373-379　2008/07

    DOI： 10.1269/jrr.07131 　

    ISSN： 0449-3060

  29. The gravity-regulated growth of axillary buds is mediated by a mechanism different from decapitation-induced release Peer-reviewed

    Daisuke Kitazawa, Yutaka Miyazawa, Nobuharu Fujii, Atsushi Hoshino, Shigeru Iida, Eiji Nitasaka, Hideyuki Takahashi

    PLANT AND CELL PHYSIOLOGY　49　(6)　891-900　2008/06

    DOI： 10.1093/pcp/pcn063 　

    ISSN： 0032-0781

  30. Immunohistochemical analysis of auxin localization during gravimorphogenesis of cucumber seedlings

    Chiaki Watanabe, Nobuharu Fujii, Yutaka Miyazawa, Hideyuki Takahashi

    International Astronautical Federation - 59th International Astronautical Congress 2008, IAC 2008　1　380-384　2008

  31. p-Chlorophenoxyisobutyric acid impairs auxin response for gravity-regulated peg formation in cucumber (Cucumis sativus) seedlings Peer-reviewed

    Minobu Shimizu, Yutaka Miyazawa, Nobuharu Fujii, Hideyuki Takahashi

    JOURNAL OF PLANT RESEARCH　121　(1)　107-114　2008/01

    DOI： 10.1007/s10265-007-0121-0 　

    ISSN： 0918-9440

  32. Correlation between development of female flower buds and expression of the CS-ACS2 gene in cucumber plants Peer-reviewed

    Sayoko Saito, Nobuharu Fujii, Yutaka Miyazawa, Seiji Yamasaki, Seiji Matsuura, Hidemasa Mizusawa, Yukio Fujita, Hideyuki Takahashi

    JOURNAL OF EXPERIMENTAL BOTANY　58　(11)　2897-2907　2007/09

    DOI： 10.1093/jxb/erm141 　

    ISSN： 0022-0957

  33. A gene essential for hydrotropism in roots Peer-reviewed

    Akie Kobayashi, Akiko Takahashi, Yoko Kakimoto, Yutaka Miyazawa, Nobuharu Fujii, Atsushi Higashitani, Hideyuki Takahashi

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　104　(11)　4724-4729　2007/03

    DOI： 10.1073/pnas.0609929104 　

    ISSN： 0027-8424

  34. Light inhibits gravity-regulated peg formation and asymmetric mRNA accumulation of auxin-inducible CsIAA1 in the cortex of the transition zone in cucumber seedlings Invited Peer-reviewed

    Nobuharu Fujii, Yuko Saito, Yutaka Miyazawa, Hideyuki Takahashi

    ADVANCES IN SPACE RESEARCH　39　(7)　1147-1153　2007

    DOI： 10.1016/j.asr.2007.01.019 　

    ISSN： 0273-1177

  35. Auxin response, but not its polar transport, plays a role in hydrotropism of Arabidopsis roots Peer-reviewed

    Tomoko Kaneyasu, Akie Kobayashi, Mayumi Nakayama, Nobuharu Fujii, Hideyuki Takahashi, Yutaka Miyazawa

    JOURNAL OF EXPERIMENTAL BOTANY　58　(5)　1143-1150　2007

    DOI： 10.1093/jxb/erl274 　

    ISSN： 0022-0957

  36. Differential accumulation of the mRNA of the auxin-repressed gene CsGRP1 and the auxin-induced peg formation during gravimorphogenesis of cucumber seedlings Peer-reviewed

    Minobu Shimizu, Keita Suzuki, Yutaka Miyazawa, Nobuharu Fujii, Hideyuki Takahashi

    PLANTA　225　(1)　13-22　2006/12

    DOI： 10.1007/s00425-006-0324-y 　

    ISSN： 0032-0935

  37. Shoot circumnutation and winding movements require gravisensing cells Peer-reviewed

    D Kitazawa, Y Hatakeda, M Kamada, N Fujii, Y Miyazawa, A Hoshino, S Iida, H Fukaki, MT Morita, M Tasaka, H Suge, H Takahashi

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　102　(51)　18742-18747　2005/12

    DOI： 10.1073/pnas.0504617102 　

    ISSN： 0027-8424

  38. Possible involvement of CS-ACS1 and ethylene in auxin-induced peg formation of cucumber seedlings Peer-reviewed

    Y Saito, S Yamasaki, N Fujii, H Takahashi

    ANNALS OF BOTANY　95　(3)　413-422　2005/02

    DOI： 10.1093/aob/mci045 　

    ISSN： 0305-7364

  39. Isolation of cucumber CsARF cDNAs and expression of the corresponding mRNAs during gravity-regulated morphogenesis of cucumber seedlings Invited Peer-reviewed

    Y Saito, S Yamasaki, N Fujii, G Hagen, T Guilfoyle, H Takahashi

    JOURNAL OF EXPERIMENTAL BOTANY　55　(401)　1315-1323　2004/06

    DOI： 10.1093/jxb/erh144 　

    ISSN： 0022-0957

  40. Gravity-induced modification of auxin transport and distribution for peg formation in cucumber seedlings: possible roles for CS-AUX1 and CS-PIN1 Peer-reviewed

    M Kamada, S Yamasaki, N Fujii, A Higashitani, H Takahashi

    PLANTA　218　(1)　15-26　2003/11

    DOI： 10.1007/s00425-003-1072-x 　

    ISSN： 0032-0935

  41. Characterization of ethylene effects on sex determination in cucumber plants Peer-reviewed

    S Yamasaki, N Fujii, H Takahashi

    SEXUAL PLANT REPRODUCTION　16　(3)　103-111　2003/09

    DOI： 10.1007/s00497-003-0183-7 　

    ISSN： 0934-0882

  42. Photoperiodic regulation of CS-ACS2, CS-ACS4 and CS-ERS gene expression contributes to the femaleness of cucumber flowers through diurnal ethylene production under short-day conditions Peer-reviewed

    S Yamasaki, N Fujii, H Takahashi

    PLANT CELL AND ENVIRONMENT　26　(4)　537-546　2003/04

    DOI： 10.1046/j.1365-3040.2003.00984.x 　

    ISSN： 0140-7791

  43. Phototropic response of Arabidopsis roots accompanies a rapid reduction of starch in columella cells Peer-reviewed

    Yamazaki Yutaka, Fujii Nobuharu, Kobayashi Akie, Takahashi Hideyuki

    Plant and Cell Physiology Supplement　2003　(0)　689-689　2003

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2003.0.689.0 　

    More details Close

    We recently found that moisture gradient or water stress immediately degrades amyloplasts in the columella cells of Arabidopsis and Raphanus roots, which caused the reduced gravitropism. Roots thus exhibit strong hydrotropism by overcoming gravitropism. It is known that not only gravitropism or hydrotropism but also phototropism play important roles in orienting Arabidopsis roots. In the present study, we examined whether degradation of amyloplasts involves in the interaction between phototropism and gravitropism. We found that phototropic response accompanied a significant reduction of starch in columella cells of Arabidopsis roots. Phototropic response and amyloplast degradation occurred due to the unilateral irradiation of white, blue or red light. Bilateral irradiation of those lights resulted in neither root bending nor reduction of starch in the columella cells. These results suggest that Arabidopsis roots display phototropism by overcoming gravitropism, due to degradation of gravisensors, amyloplasts, in the columella cells, as does hydrotropism.

  44. Isolation and characterization of hydrotropic mutants in Arabidopsis roots Peer-reviewed

    Kobayashi Akie, Kakimoto Yoko, Yamazaki Yutaka, Fujii Nobuharu, Okada Kiyotaka, Takahashi Hideyuki

    Plant and Cell Physiology Supplement　2003　(0)　95-95　2003

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2003.0.95.0 　

    More details Close

    Roots display positive hydrotropism in response to a moisture gradient, which regulates the directional growth by interactions with other tropisms such as gravitropism. To dissect the molecular mechanism underlying hydrotropism in seedling roots, we established a method for screening Arabidopsis mutants defective in root hydrotropism, and we have successfully obtained 12 mutants of which root hydrotropism is reduced to various extents. Among them, roots of a mutant named rhy1 (root hydrotropism 1) were found to be unique because they showed ahydrotropic response although their growth rate was normal, and they normally displayed gravitropism, phototropism and waving response. Roots of other rhy mutants (rhy2, rhy3, rhy4) showed the reduced hydrotropism together with either a reduced gravitropism or a reduced waving response. Currently, we are attempting to clarify factor(s) responsible for hydrotropism by identifying the mutated genes in the rhy<br> mutants.

  45. Morphogenesis, hydrotropism, and distribution of auxin signals in cucumber seedlings grown in microgravity Invited

    Takahashi H, Kamada M, Saito Y, Kobayashi A, Higashitani A, Fujii N

    Plant Biotechnology 2002 and Beyond　359-362　2003

  46. [Gravity-induced asymmetry of localization of auxin-carrier proteins (CS-AUX1 and CS-PIN1) for peg formation in cucumber seedlings]. Peer-reviewed

    Kamada M, Fujii N, Higashitani A, Takahashi H

    Uchu Seibutsu Kagaku　16　(3)　153-154　2002/11

    ISSN： 0914-9201

  47. Hydrotropic response and expression pattern of auxin-inducible gene, CS-IAA1, in the primary roots of clinorotated cucumber seedlings Invited Peer-reviewed

    H Mizuno, A Kobayashi, N Fujii, M Yamashita, H Takahashi

    PLANT AND CELL PHYSIOLOGY　43　(7)　793-801　2002/07

    DOI： 10.1093/pcp/pcf093 　

    ISSN： 0032-0781

  48. Molecular approach to the study of sex determination in cucucmber plants Peer-reviewed

    S. Yamasaki, N. Fujii, H. Takahashi, H. Mizusawa, S. Matsuura

    Acta Horticulturae　588　309-312　2002

    Publisher: International Society for Horticultural Science

    DOI： 10.17660/ActaHortic.2002.588.49 　

    ISSN： 0567-7572

  49. A role for auxin in gravity's negative control of morphogenesis in cucumber seedlings Peer-reviewed

    M Kamada, N Fujii, H Takahashi, S Aizawa, S Kamigaichi, C Mukai, T Shimazu

    PROCEEDINGS OF THE IIND INTERNATIONAL SYMPOSIUM ON CUCURBITS　(588)　49-50　2002

    ISSN： 0567-7572

  50. [Auxin distribution and transport in the transition zone (peg region) between hypocotyl and root of cucumber seedlings]. Peer-reviewed

    Kamada M, Fujii N, Higashitani A, Takahashi H

    Uchu Seibutsu Kagaku　15　(3)　244-245　2001/10

    ISSN： 0914-9201

  51. [Analysis of circumnutation in the seedlings (correction of seedings) of agravitropic mutants]. Peer-reviewed

    Hatakeda Y, Takahashi N, Kamada M, Fujii N, Higashitani A, Suge H, Fukaki H, Tasaka M, Goto N, Takahashi H

    Uchu Seibutsu Kagaku　15　(3)　250-251　2001/10

    ISSN： 0914-9201

  52. The M locus and ethylene-controlled sex determination in andromonoecious cucumber plants Peer-reviewed

    S Yamasaki, N Fujii, S Matsuura, H Mizusawa, H Takahashi

    PLANT AND CELL PHYSIOLOGY　42　(6)　608-619　2001/06

    DOI： 10.1093/pcp/pce076 　

    ISSN： 0032-0781

  53. [The gravity-regulated formation of peg and auxin transport in cucumber seedlings]. Peer-reviewed

    Kamada M, Sakata T, Fujii N, Higashitani A, Takahashi H

    Uchu Seibutsu Kagaku　14　(3)　240-241　2000/10

    ISSN： 0914-9201

  54. Control of gravimorphogenesis by auxin: accumulation pattern of CS-IAA1 mRNA in cucumber seedlings grown in space and on the ground Peer-reviewed

    M Kamada, N Fujii, S Aizawa, S Kamigaichi, C Mukai, T Shimazu, H Takahashi

    PLANTA　211　(4)　493-501　2000/09

    DOI： 10.1007/s004250000321 　

    ISSN： 0032-0935

  55. The ethylene-regulated expression of CS-ETR2 and CS-ERS genes in cucumber plants and their possible involvement with sex expression in flowers Peer-reviewed

    S Yamasaki, N Fujii, H Takahashi

    PLANT AND CELL PHYSIOLOGY　41　(5)　608-616　2000/05

    ISSN： 0032-0781

  56. Differential accumulation of Aux/IAA mRNA during seedling development and gravity response in cucumber (Cucumis sativus L.) Peer-reviewed

    N Fujii, M Kamada, S Yamasaki, H Takahashi

    PLANT MOLECULAR BIOLOGY　42　(5)　731-740　2000/03

    DOI： 10.1023/A:1006379804678 　

    ISSN： 0167-4412

  57. Morphogenesis in cucumber seedlings is negatively controlled by gravity Peer-reviewed

    H Takahashi, M Kamada, Y Yamazaki, N Fujii, A Higashitani, S Aizawa, Yoshizaki, I, S Kamigaichi, C Mukai, T Shimazu, K Fukui

    PLANTA　210　(3)　515-518　2000/02

    DOI： 10.1007/s004250050039 　

    ISSN： 0032-0935

  58. A spaceflight experiment for the study of gravimorphogenesis and hydrotropism in cucumber seedlings Peer-reviewed

    H Takahashi, H Mizuno, M Kamada, N Fujii, A Higashitani, S Kamigaichi, S Aizawa, C Mukai, T Shimazu, K Fukui, M Yamashita

    JOURNAL OF PLANT RESEARCH　112　(1108)　497-505　1999/12

    DOI： 10.1007/PL00013906 　

    ISSN： 0918-9440

  59. Endoxyloglucan transferase cDNA isolated from pea roots and its fluctuating expression in hydrotropically responding roots Peer-reviewed

    M Takano, N Fujii, A Higashitani, K Nishitani, T Hirasawa, H Takahashi

    PLANT AND CELL PHYSIOLOGY　40　(2)　135-142　1999/02

    ISSN： 0032-0781

  60. Agravitropic mutant for the study of hydrotropism in seedling roots Peer-reviewed

    H. Takahashi, M. Takano, N. Fujii, A. Higashitani, M. Yamashita, T. Hirasawa, K. Nishitani

    Advances in Space Research　23　(12)　2021-2028　1999

    Publisher: Elsevier Ltd

    DOI： 10.1016/S0273-1177(99)00341-5 　

    ISSN： 0273-1177

  61. A role of cytoskeletal structure of cortical cells in the gravity-regulated formation of a peg in cucumber seedlings Peer-reviewed

    M Kobayashi, T Murata, N Fujii, M Yamashita, A Higashitani, H Takahashi

    LIFE SCIENCES: MICROGRAVITY RESEARCH II　24　(6)　771-773　1999

    ISSN： 0273-1177

  62. Pattern of DNA binding of nuclear proteins to the proximal Agrobacterium rhizogenes rolC promoter is altered during somatic embryogenesis of carrot Peer-reviewed

    N Fujii

    GENE　201　(1-2)　55-62　1997/11

    DOI： 10.1016/S0378-1119(97)00427-7 　

    ISSN： 0378-1119

  63. Molecular and biochemical characterization of a proteasome subunit from rice and carrot cells Peer-reviewed

    M Umeda, N Fujii, Y Manabe, H Uchimiya

    MOLECULAR & GENERAL GENETICS　255　(1)　19-27　1997/06

    DOI： 10.1007/s004380050470 　

    ISSN： 0026-8925

  64. Induction of hydrotropism in clinorotated seedling roots of Alaska pea, Pisum sativum L Peer-reviewed

    H Takahashi, M Takano, N Fujii, M Yamashita, H Suge

    JOURNAL OF PLANT RESEARCH　109　(1095)　335-337　1996/09

    DOI： 10.1007/BF02344481 　

    ISSN： 0918-9440

  65. IMMUNOLOGICAL ANALYSIS OF A SMALL GTP-BINDING PROTEIN IN HIGHER-PLANT CELLS Peer-reviewed

    T ANAI, ET ASPURIA, N FUJII, T UEDA, M MATSUI, K HASEGAWA, H UCHIMIYA

    JOURNAL OF PLANT PHYSIOLOGY　147　(1)　48-52　1995/10

    ISSN： 0176-1617

  66. Phenotypic instability of transgenic tobacco plants and their progenies expressing Arabidopsis thafiana small GTP-binding protein genes Peer-reviewed

    Evalour T. Aspuria, Toyoaki Anai, Nobuharu Fujii, Takashi Ueda, Masahiro Miyoshi, Minami Matsui, Hirofumi Uchimiya

    MGG Molecular &amp; General Genetics　246　(4)　509-513　1995/07

    Publisher: Springer-Verlag

    DOI： 10.1007/BF00290454 　

    ISSN： 0026-8925 1617-4623

  67. THE ROLC PROMOTER OF AGROBACTERIUM-RHIZOGENES RI PLASMID IS ACTIVATED BY SUCROSE IN TRANSGENIC TOBACCO PLANTS Peer-reviewed

    R YOKOYAMA, T HIROSE, N FUJII, ET ASPURIA, A KATO, H UCHIMIYA

    MOLECULAR & GENERAL GENETICS　244　(1)　15-22　1994/07

    DOI： 10.1007/BF00280182 　

    ISSN： 0026-8925

  68. ANALYSIS OF THE ROLC PROMOTER REGION INVOLVED IN SOMATIC EMBRYOGENESIS-RELATED ACTIVATION IN CARROT CELL-CULTURES Peer-reviewed

    N FUJII, R YOKOYAMA, H UCHIMIYA

    PLANT PHYSIOLOGY　104　(4)　1151-1157　1994/04

    DOI： 10.1104/pp.104.4.1151 　

    ISSN： 0032-0889

  69. CONDITIONS FAVORABLE FOR THE SOMATIC EMBRYOGENESIS IN CARROT CELL-CULTURE ENHANCE EXPRESSION OF THE ROLC PROMOTER-GUS FUSION GENE Peer-reviewed

    N FUJII, H UCHIMIYA

    PLANT PHYSIOLOGY　95　(1)　238-241　1991/01

    ISSN： 0032-0889

Show all ︎Show first 5

Misc. 100

  1. 2step‐TILLINGを用いた自家不和合植物ハツカダイコンの突然変異体選抜法

    上妻馨梨, 千葉元子, 千葉元子, 穴井豊昭, 上田実希, 小口理一, 花田耕介, 花田耕介, 彦坂幸毅, 彦坂幸毅, 藤井伸治, 藤井伸治

    育種学研究　19　16　2017/03

    ISSN： 1344-7629

  2. 自然変異に学ぶ適応のしくみ: アブラナ科植物の種内変異を用いたアプローチ

    彦坂幸毅, 森長真一, 小口理一, 永野聡一郎, 見塩昌子, 上妻馨梨, 藤井伸治, 須藤恵美, 石塚航, 田島直幸, 尾崎洋史, Wang Q-W, 花田耕介

    育種学研究　19　24-25　2017

  3. 重力屈性が関与するイネ幼葉鞘の回旋転頭運動:lazy 1突然変異体と宇宙実験による解析

    小林啓恵, 冨田優太, 金慧正, 藤井伸治, 宮沢豊, 矢野幸子, 山崎千秋, 鎌田源司, 笠原春夫, 嶋津徹, 伏島康男, 高橋秀幸

    日本植物学会大会研究発表記録　80th　202　2016/09/01

  4. イネ幼葉鞘の回旋転頭運動における重力応答とその宇宙実験による検証

    小林啓恵, 冨田優太, 金慧正, 藤井伸治, 宮沢豊, 矢野幸子, 山崎千秋, 鎌田源司, 笠原春夫, 嶋津徹, 嶋津徹, 伏島康男, 高橋秀幸

    日本宇宙航空環境医学会大会プログラム・予稿集　62nd　103　2016

  5. 9. L-Glutamate plays an important role in early hydrotropic response in Arabidopsis thaliana

    Iwata Satoru, Fujii Nobuharu, Kobayashi Akie, Takahashi Hideyuki

    48　24-24　2013/10/04

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

  6. 8. Root hydrotropism as affected by inhibitors of auxin transport, action and biosynthesis in rice seedlings

    Nakajima Yusuke, Fujii Nobuharu, Miyazawa Yutaka, Kobayashi Akie, Tkahashi hideyuki

    48　23-23　2013/10/04

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

  7. 微小重力下で亢進されるキュウリ根の水分屈性

    高橋秀幸, 諸橋恵太, 岡本美貴, 山崎千秋, 藤井伸治, 宮沢豊, 東端晃, 嶋津徹, 鎌田源司, 笠原春夫, 山崎丘, 長田郁子, 石岡憲昭, 伏島康男, 小林啓恵

    日本植物学会大会研究発表記録　77th　182　2013/08/20

  8. Polar Auxin Transport (eds by R. Chen, F. Baluska)

    Yamazaki C, Fujii N, Takahashi H

    2013/02/12

    Publisher: Springer

    DOI： 10.1007/978-3-642-35299-7_9 　

  9. The role of auxin transport and distribution in plant gravimorphogenesis Invited

    Yamazaki C, Fujii N, Takahashi H

    Polar Auxin Transport (eds by R. Chen, F. Baluska) Springer　179-199　2013

  10. キュウリ芽生えの内皮重力感受細胞で発現するCsPIN1タンパク質の蓄積の重力応答性

    渡邉千秋, 藤井伸治, 宮沢豊, 東端晃, 嶋津徹, 鎌田源司, 笠原春夫, 山崎丘, 渡邉郁子, 伏島康男, 石岡憲昭, 高橋秀幸

    日本植物学会大会研究発表記録　76th　128　2012/09/14

  11. Ethylene regulation of sex differentiation of flowers in cucurbitaceous plants and its genetic model

    Yamasaki Seiji, Manabe Kazuto, Fujii Nobuharu, Takahashi Hideyuki

    Regulation of Plant Growth & Development　47　(1)　24-33　2012/05/31

    Publisher: The Japanese Society for Chemical Regulation of Plants

    DOI： 10.18978/jscrp.47.1_24 　

    ISSN： 1346-5406

    More details Close

    The mechanism of sex differentiation in cucurbitaceous plants has been studied for more than 80 years. Sex expression in cucumber (Cucumis sativus L.) plants is genetically controlled by the F and M genes. Similarly, it is controlled by the A and G genes in melon (Cucumis melo L.) plants. These genes interact to produce four different sex phenotypes; gynoecious (M-F-) (A-gg), monoecious (M-ff) (A-G-), hermaphroditic (mmF-) (aagg), and andromonoecious (mmff) (aaG-). The gynoecious type produces only female flowers, whereas the monoecious type produces both male and female flowers on the same plant. The hermaphroditic type produces bisexual flowers with both staminate and pistillate organs, while the andromonoecious type produces bisexual and male flowers on the same plant. Sex expression in both cucumber and melon plants is mainly controlled by a plant hormone, ethylene. Thus, the mechanism of sex expression may be similar or even identical in cucumber and melon plants. The F gene was identified more than 10 years ago, whereas the M, A, and G genes were identified recently. The F, M, and A genes encode ACC synthase, which catalyzes the production of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) from S-adenosyl methionine (SAM). This article reviews the similarities and differences in the mechanism of sex expression in cucumber and melon plants and proposes a genetic model of sex expression.

  12. 53. MIZ1 integrates light and ABA signal so as to regulate hydrotropic response adequately

    Moriwaki Teppei, Kobayashi Akie, Miyazawa Yutaka, Fujii Nobuharu, Takahashi Hideyuki

    (45)　70-70　2010/10/01

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

    More details Close

    Roots show tropisms in response to environmental cues such as gravity, light, moisture gradient. Each tropism is affected by the other stimulations. For instance, graviresponse interferes root hydrotropism and phototropism. We have identified MIZ1 gene as a regulator of hydrotropism. However, regulation and function of MIZ1 have been unknown. Here, we showed that light and ABA signal affect hydrotropism via modulation of MIZ1 expression. We found that hydrotropism was reduced in dark-grown roots when compared with light-grown ones. In addition, we found that light signal was necessary for MIZ1-GFP expression at the root cap. Moreover, both of hydrotropism and MIZ1 expression are decreased in hy5 mutant. Because HY5 regulates light-dependent transcription, the result suggests that HY5-dependent light signaling is required for expression of MIZ1 and thus for appropriate hydrotropic response. It is also known that HY5 is involved in ABA signaling, and that ABA is an important player for hydrotropism. To assess the possibility that HY5 also regulates ABA-mediated MIZ1 expression, we analyzed MIZ1-GFP level in ABA-treated hy5 roots. The result shows that ABA could recover MIZ1-GFP level similar to that of WT. Moreover, ABA-treated hy5 showed normal hydrotropic response as did ABA-treated WT. These results suggest that HY5 is not involved in ABA-dependent regulation of hydrotropism which acts even in darkness. These machineries may profit for plants to acquire water adequately.

  13. Strategy of Frontier Organisms: Graviperception Systems for Plant Growth and

    Takahashi Hideyuki, Iino M., Ueda Junichi, Kamada Motoshi, Kamisaka Seiichiro, Kaneko Yasuko, Kitaya Yoshiaki, Soga Kouichi, Tasaka Masao, Fujii Nobuharu, Hoson Takayuki, Miyazawa Yutaka, Miyamoto Kensuke, Murata Takashi, Morita M., Yamashita Masamichi

    (26)　2010/02

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)

    More details Close

    Studies of our working group are aimed at understanding the graviperception mechanism and its interactions with mechanisms of other gravity-influenced phenomena of plant growth and development. Our collaborative works will bring about hypotheses on the molecular mechanisms underlying plant responses to gravity, which will be further verified by spaceflight experiments. This approach will also lead to the establishment of technology useful for controlling plant growth and development in space. We have continued our studies to reveal mechanisms underlying graviperception and its signal transduction in plants by using gravitropic mutants, and found various genes and molecules responsible for the mutations. Also, we have studied the gravity-influenced growth and development of plants; namely, gravimorphogenesis of cucumber seedlings, shoot circumnutation, and hydrotropism in seedling roots. The results obtained suggested molecular cascades for graviresponse and molecular networks for the interactions among the gravity-influenced phenomena. Based on these accomplishments, we are attempting to establish models and hypotheses to be verified by spaceflight experiments. Some of the subjects being dealt in this project are to be flown in this year. Currently, we are preparing to propose another spaceflight experiment on circumnutation and its relationship to graviresponse in plants.joint hosting: The Science Council of Japan

  14. 「Hydro Tropi」宇宙実験へ向けた給水システムのパラボリックフライトによる検証

    宮沢豊, 岡本美貴, 藤井伸治, 高橋秀幸, 石岡憲昭, 山崎丘, 鎌田源司, 嶋津徹

    宇宙利用シンポジウム　26th　2010

  15. キュウリ芽生えの重力応答によりネガティブに制御される形態形成におけるオーキシン勾配制御

    藤井伸治, 渡辺千秋, 矢内健一, 宮沢豊, 高橋秀幸, 西村岳志, 小柴共一, 山崎丘, 石岡憲昭, 鎌田源司, 嶋津徹

    宇宙利用シンポジウム　26th　2010

  16. 18. Changes in CsPIN1 localization and IAA distribution during gravimorphogenesis of cucumber seedlings

    Watanabe Chiaki, Fujii Nobuharu, Nishimura Takeshi, Miyazawa Yutaka, Koshiba Tomokazu, Takahashi Hideyuki

    (44)　32-32　2009/10/06

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

    More details Close

    When cucumber seeds germinate in a horizontal position, resulting seedlings develop a specialized protuberance, termed the peg, on the lower side of the root-hypocotyl transition zone, due to gravistimulation. We have shown that exogenously applied auxin induces peg formation and that content of a free form of auxin, indole-3-acetic acid (IAA), in the upper side of the gravistimulated transition zone is less than that in the lower side. In addition, we have shown that auxin efflux carrier CsPIN1 proteins are expressed in endodermal cells and after 120min gravistimulation, the localization pattern of CsPIN1 changed to transport more auxin to lower side than upper side. To reveal the contribution of CsPIN1 to auxin dynamics during gravistimulation in the transition zone, we analyzed the content and distribution of endogenous IAA in the gravistimulated transition zone. After 30min gravistimulation, a significant accumulation in IAA content was detected in lower side of transition zone compared with its upper side. In addition, our immunohistochemical analysis using anti-IAA antibody revealed that the distribution of IAA-signals changed within 2min after gravistimulation. These results suggest that during peg formation, the change in localization pattern of CsPIN1 due to gravistimulation has the secondary effect and the other mechanisms exist to induce an immediate asymmetric IAA distribution in the transition zone of cucumber seedlings.

  17. Strategy of frontier organisms: Graviperception systems for plant growth and development

    Takahashi Hideyuki, Ueda Junichi, Kamada Motoshi, Kamisaka Seiichiro, Kaneko Yasuko, Kitaya Yoshiaki, Soga Kouichi, Tasaka Masao, Fujii Nobuharu, Hoson Takayuki, Miyazawa Yutaka, Miyamoto Kensuke, Murata Takashi, Yamashita Masamichi

    (25)　2009/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)

    More details Close

    Studies of our working group are aimed at understanding the graviperception mechanism and its interactions with other gravity-influenced phenomena of growth and development of plants. Our collaborative works will bring about hypotheses on the molecular mechanisms underlying plant responses to gravity, which will be further verified by spaceflight experiments. This approach will also lead to the establishment of technology useful for controlling plant growth and development in space. We continued our studies to reveal mechanisms underlying graviperception in plants, and based on microarray analysis of a gravitropic mutant and its wild type Arabidopsis and newly screened gravitropic mutants of Arabidopsis we obtained novel graviresponse-related genes. In addition, we utilized two agravitropic mutants of morning glory defective in gravisensing cell differentiation and found that graviperception was involved in the bending-induced growth of lateral shoots and that the mechanisms underlying gravity- and decapitation-regulated release from apical dominance were distinct and unique. Furthermore, we studied automorphogenesis of pea epicotyls and hydrotropism in Arabidopsis roots, as gravity-influenced growth responses in plants. The results provided us with a clue to understand the interaction between automorphogenesis and gravitropism in relation to auxin transport. Also, identification of genes responsible for ahydrotropism of Arabidopsis mutant revealed molecular mechanisms unique to hydrotropism but independent of gravitropism.

  18. 第5回ライフサイエンス国際公募宇宙実験選定テーマ「Hydro Tropi」の実施へ向けた実験系の調整

    宮沢豊, 岡本美貴, 藤井伸治, 高橋秀幸, 石岡憲昭, 山崎丘, 鎌田源司, 嶋津徹

    宇宙利用シンポジウム　25th　2009

  19. 33. Mechanism of deep-sowing tolerance in wheat : the tissue specific effect of GA on first internode elongation

    Kato Fumie, Miyazawa Yutaka, Araki Masaru, Fujii Nobuharu, Suge Hiroshi, Takeda Kazuyoshi, Takahashi Hideyuki

    (43)　47-47　2008/10/06

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

    More details Close

    Wheat seedlings elongate their first internode to elevate the shoot apical meristem toward the soil surface when seeds are sown deep in the soil. We have shown that Hong Mang Mai wheat (HMM) elongates its first internode at an extreme rate and is able to emerge from much deeper soil than do others because of its super-responsiveness to GA. To understand the mechanisms underlying the deep-sowing tolerance of HMM, we investigated the effect of GA on cell elongation and cell proliferation in the first internode of HMM and compared them to those of ordinary cultivar with altered responsiveness to GA, namely Hope. In both HMM and Hope, GA treatment hardly affected cell length in cortical tissue, whereas its cell number increased in response to GA. In epidermis of the GA-treated first internodes, on the other hand, cell elongation was accelerated, whereas there was no substantial change in cell number. The enhanced rates of elongation of epidermal cells and proliferation of cortical cells due to GA treatment was greater in HMM, compared to those in Hope. These results suggest that in the GA-treated seedlings of wheat elongation of epidermal cells and proliferation of cortical cells collaborate for making first internode longer. The first internodes of HMM may possess the pronounced GA effects on cell elongation and proliferation, which could exert tolerance to deep-sowing conditions.

  20. 78. Changes in IAA distribution during gravimorphogenesis of cucumber seedlings

    Watanabe Chiaki, Fujii Nobuharu, Miyazawa Yutaka, Takahashi Hideyuki

    (43)　92-92　2008/10/06

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

    More details Close

    When cucumber seeds are germinated in a horizontal position, resulting seedlings develop a specialized protuberance, termed the peg, on the lower side of the root-hypocotyl transition zone (TR zone), because gravistimulation suppresses peg formation on the upper side of it. We have shown that 2min of gravistimulation is sufficient for the suppression of peg formation. Also, we have shown that redistribution of indole-3-acetic acid (IAA) plays an important role in regulation of this gravimorphogenesis. However, we do not know whether auxin redistribute within 2min following gravistimulation. To verify the possibility of this rapid redistribution of IAA upon gravistimulation, we investigated it by immunofluorescence method using anti-IAA monoclonal antibody. When the excised TR zone was treated with IAA, immunofluorescent signals of IAA were detected at higher intensity than those in the IAA-starved segments. These results indicated that our immunofluorescent analysis was valid for monitoring IAA. When cucumber seedlings grown in a vertical position were gravistimulated by orienting them in a horizontal position, distribution of IAA-signals changed within 2min after gravistimulation. The intensity increased in nuclei and cytoplasm of epidermal cells in the lower side of the TR zone, compared to that in the upper side. Thus, IAA redistribution commences to occur within 2min could be responsible for the gravity-regulated suppression of peg formation in cucumber seedlings.

  21. 79. Identification and immunohistochemical analysis of PIN homologues expressing in the transition zone of cucumber seedlings

    Fujii Nobuharu, Yanai Kenichi, Hotta Takuya, Watanabe Chiaki, Miyazawa Yutaka, Takahashi Hideyuki

    (43)　93-93　2008/10/06

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

    More details Close

    In Cucurbitaceae plants, a protuberance called a peg is formed on the transition zone between the hypocotyl and the root. When cucumber seeds germinate in a horizontal position, a peg forms on the lower side of the transition zone. We have suggested that auxin efflux is required for the differential decrease in auxin level in the gravistimulated transition zone of cucumber. To reveal a mechanism underlying the gravity-controlled peg formation, it is important to identify auxin efflux facilitators that are expressed in endodermis of the transition zone of cucumber seedlings. In this study, we isolated cDNAs encoding PIN auxin efflux facilitator family protein (CsPIN1? CsPIN6) from cucumber and examined the corresponding mRNA accumulation in the transition zone of cucumber seedlings. Our results of in situ hybridization indicate that CsPIN1, CsPIN4 and CsPIN6 mRNAs accumulate abundantly in endodermis/xylem, xylem and phloem cells of the transition zone, respectively. Furthermore, immunohistochemical staining with anti-CsPIN1 antibodies produced signals in vascular tissues and endodermis. Because endodermal cells have been reported to be responsible for sensing gravity for the lateral placement of the peg, CsPIN1 in endodermis could contribute to auxin redistribution that ultimately causes the suppression of peg formation on the upper side of the gravistimulated seedlings of cucumber.

  22. Strategy of frontier organisms: Graviperception systems for plant growth and development

    Takahashi Hideyuki, Ueda Junichi, Kamada Motoshi, Kamisaka Seiichiro, Kaneko Yasuko, Kitaya Yoshiaki, Soga Koichi, Tasaka Masao, Fujii Nobuharu, Hoson Takayuki

    401-404　2008/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)

    More details Close

    Studies of our working group are aimed at understanding the graviperception mechanism and its interactions with other gravity-influenced phenomena of growth and development of plants. Our collaborative works will bring about hypotheses on the molecular mechanisms underlying plant responses to gravity, which will be further verified by spaceflight experiments. This approach will also lead to the establishment of technology useful for controlling plant growth and development in space. Our achievements in this year include identification of novel genes responsible for gravitropic response in shoots and roots, finding the roles of auxin efflux carriers in automorphogenesis, gravimorphogenesis (peg formation) and hydrotropism, and demonstrating the gravity-dependent release from apical dominance.

  23. Analysis of auxin-responsive gene expression on gravimorphogenesis of cucumber seedlings

    SHIMIZU Minobu, MIYAZAWA Yutaka, FUJII Nobuharu, TAKAHASHI Hideyuki

    42　18-18　2007/10/05

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 1346-5406

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    Auxin is an important plant hormone for morphogenesis. Cucumber seedlings form a specialized protuberance, called peg, on the transition zone (TR-zone) between the hypocotyl and root. When cucumber seeds germinate in a horizontal position, seedlings develop a peg on the lower side of the TR-zone. We previously demonstrated that auxin was more abundant in the lower side than in the upper side of the gravistimulated TR-zone and suggested that this asymmetric auxin distribution induced differential auxin response to contribute to peg formation. Moreover, application of auxin or auxin-polar-transport inhibitor (TIBA) induced both expression of auxin-inducible gene expression and peg formation at both upper and lower side of the TR-zone, whereas application of anti-auxin (PCIB) induced neither. Recently, we found CsGRPl whose mRNA accumulated more abundantly in the upper side than in the lower side of the TR-zone. We found that this accumulation of CsGRPl mRNA was decreased by the application of either auxin or TIBA. On the other hand, application of PCIB induced CsGRPl gene expression in both upper and lower side of the TR-zone. These results suggest that gravistimulation increases not only the expression of auxin-inducible genes on the lower side of the TR-zone but also the expression of auxin-repressed genes, such as CsGRPl, on the upper side in cucumber seedlings to contribute to the one-sided formation of the peg.

  24. Regulation of auxin efflux is necessary for root hydrotropism in cucumber seedlings

    MIYAZAWA Yutaka, MOROHASHI Keita, FUJII Nobuharu, TAKAHASHI Hideyuki

    42　17-17　2007/10/05

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 1346-5406

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    Hydrotropism is a response of root to a moisture gradient. Because of the necessity of water acquisition, root hydrotropism has been considered as an important tropism. Recent analyses using Arabidopsis have emphasized the contribution of polar auxin transport to differential auxin distribution as well as to gravitropism. Our previous results also suggested that differential auxin distribution plays a pivotal role in root hydrotropism. Nonetheless, how auxin distribution is controlled in this phenomenon remains unclear. In search of the molecules that control auxin redistribution in hydrotropic response, we found that inhibitors of auxin efflux significantly reduced hydrotropism in cucumber seedlings. Moreover, our immunohistochemical analyses revealed that the expression of putative auxin efflux facilitator CsPIN5 was diminished at the dry side of the distal elongation zone of hydrotropically responding roots, whereas it was maintained at the corresponding region of the humid side. Considering the previous results that auxin-responsive gene expression is mainly occurred at the humid side of distal elongation zone of cucumber seedlings, our present results suggests that CsPIN5-mediated auxin efflux plays definite role in regulating auxin redistribution during hydrotropic response in cucumber seedlings.

  25. Comparative analysis of mechanisms for axillary bud growth induced by either gravistimulation or decapitation

    KITAZAWA Daisuke, MIYAZAWA Yutaka, FUJII Nobuharu, TAKAHASHI Hideyuki

    42　19-19　2007/10/05

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 1346-5406

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    The leading shoot dominates the growth of the axillary buds. After decapitation of the shoot apex, outgrowth of axillary buds begins. This phenomenon is known as apical dominance. Auxin basipetally derived from apical bud has an inhibitory effect on the growth of axillary buds, whereas cytokinin promotes axillary bud outgrowth. It is also proposed that graviresponse is involved in apical dominance. When the upper part of main shoot of morning glory (Pharbitis nil) is bent down, an axillary bud situated on the uppermost node starts to elongate. Using an agravitropic mutant of morning glory, weeping, we previously demonstrated that this release from apical dominance requires graviresponse. However, the molecular mechanism of this phenomenon is unclear. In this report, we examined the involvement of cytokinin and auxin in gravity-regulated axillary bud growth in comparison with that induced by decapitation. The newly isolated cytokinin biosynthetic gene, PnIPT2, was up-regulated by decapitation, whereas it was suppressed by auxin exogenously treated to the stump. PnIAAl, an auxin inducible gene, was down-regulated by decapitation. In contrast, neither PnIPT2 nor PnIAAl expression changed with shoot bending treatment. These results suggest that mechanism for gravity-influenced release from apical dominance is independent of that of decapitation.

  26. Strategy of frontier organisms: Graviperception systems for plant growth and development

    Takahashi Hideyuki, Ueda Junichi, Kamada Motoshi, Kamisaka Seiichiro, Kaneko Yasuko, Kitaya Yoshiaki, Soga Koichi, Takahashi Hideyuki, Tasaka Masao, Fujii Nobuharu

    367-370　2007/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)

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    Studies of our working group are aimed at understanding the graviperception mechanism and its interactions with other gravity-influenced phenomena of growth and development of plants. Our collaborative works will bring about hypotheses on the molecular mechanisms underlying plant responses to gravity, which will be further verified by spaceflight experiments. This approach will also lead to the establishment of technology useful for controlling plant growth and development in space. Here, we report our accomplishments in 2006, which includes studies on the roles of cytoskeleton in graviresponse/graviperception, novel gravitropic mutants, genes responsible for gravitropism, auxin transport system involved in gravitropism and automorphogenesis, circumnutation and release from apical dominance that require graviperception, and molecular pathways that work for hydrotropism independently of gravitropism.

  27. Physiological analysis on the mechanisms of gravityregulated axillary bud growth

    Daisuke Kitazawa, Yutaka Miyazawa, Nobuharu Fujii, Hideyuki Takahashi

    PLANT AND CELL PHYSIOLOGY　48　S161-S161　2007

    ISSN： 0032-0781

  28. CsPIN1 localization pattern modified by gravistimulation in endodermis of cucumber seedlings

    Nobuharu Fujii, Kenichi Yanai, Takuya Hotta, Yutaka Miyazawa, Hideyuki Takahashi

    PLANT AND CELL PHYSIOLOGY　48　S162-S162　2007

    ISSN： 0032-0781

  29. Distinctive auxin regulation in root hydrotropism of Arabidopsis thaliana

    Tomoko Kaneyasu, Akie Kobayashi, Mayumi Nakayama, Nobuharu Fujii, Hideyuki Takahashi, Yutaka Miyazawa

    PLANT AND CELL PHYSIOLOGY　48　S200-S200　2007

    ISSN： 0032-0781

  30. A novel gene responsible for hydrotropism in Arabidopsis roots

    Akie Kobayashi, Akiko Takahashi, Yoko Kakimoto, Yutaka Miyazawa, Nobuharu Fujii, Atsushi Higashitani, Hideyuki Takahashi

    PLANT AND CELL PHYSIOLOGY　48　S144-S144　2007

    ISSN： 0032-0781

  31. Effect of anti-auxin, PCIB, on gravirnorphogenesis of cucumber seedlings

    Minobu Shimizu, Yutaka Miyazawa, Nobuharu Fujii, Hideyuki Takahashi

    PLANT AND CELL PHYSIOLOGY　48　S162-S162　2007

    ISSN： 0032-0781

  32. キュウリ根の水分屈性におけるオーキシンとオーキシン排出キャリアの役割

    諸橋 恵太, 柿本 洋子, 宮沢 豊, 藤井 伸治, 高橋 秀幸

    根の研究 = Root research　15　(4)　2006/12/29

    ISSN： 0919-2182

  33. Characterization of Arabidopsis root hydrotropic mutant, miz4

    OOBA Atsushi, MIYAZAWA Yutaka, KOBAYASHI Akie, TAKAHASHI Akiko, FUJII Nobuharu, TAKAHASHI Hideyuki

    119　86-87　2006/12/01

    ISSN： 0918-9440

  34. Identification of a novel gene for hydrotropism in Arabidopsis

    KOBAYASHI Akie, TAKAHASHI Akiko, KAKIMOTO Yoko, MIYAZAWA Yutaka, FUJII Nobuharu, TAKAHASHI Hideyuki

    119　68-68　2006/12/01

    ISSN： 0918-9440

  35. Differential accumulation of CsARF5 protein during gravity-regulated morphogenesis of cucumber seedlings

    SAITO Yuko, MIYAZAWA Yutaka, FUJII Nobuharu, TAKAHASHI Hideyuki

    119　77-77　2006/12/01

    ISSN： 0918-9440

  36. Analyses of genes whose transcript levels are regulated during root hydrotropic response in Arabidopsis

    MIYAZAWA Yutaka, OOBA Atsushi, KOBAYASHI Akie, NAKAYAMA Mayumi, FUJII Nobuharu, TAKAHASHI Hideyuki

    119　87-87　2006/12/01

    ISSN： 0918-9440

  37. Characteristics of cell division and cell elongation that confer deep-seeding tolerance in wheat cultivar Hong Mang Mai

    KATO Fumie, MIYAZAWA Yutaka, ARAKI Masaru, FUJII Nobuharu, SUGE Hiroshi, TAKEDA Kazuyoshi, TAKAHASHI Hideyuki

    119　160-161　2006/12/01

    ISSN： 0918-9440

  38. 根の水分屈性と重力屈性に機能する細胞群とオーキシン

    金安 智子, 根岸 洋, 宮沢 豊, 仲村 和子, 藤井 伸治, 柿本 洋子, 高橋 秀幸

    根の研究 = Root research　15　(2)　72-72　2006/06/28

    ISSN： 0919-2182

  39. Strategy of Frontier Organisms: Graviperception Systems for Plant Growth and Development

    Ueda Junichi, Kamada Motoshi, Kamisaka Seiichiro, Kaneko Yasuko, Kitaya Yoshiaki, Soga Kouichi, Takahashi Hideyuki, Tasaka Masao, Fujii Nobuharu, Hoson Takayuki, Miyazawa Yutaka, Miyamoto Kensuke, Murata Takashi, Yamashita Masamichi

    (22)　2006/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)

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    Terrestrial plants control their orientation of growth for obtaining water and nutrients, harvesting light for photosynthesis, and anchoring themselves. This is the feature fundamentally important for plants to survive under various environmental conditions and to achieve efficient production of carbohydrates as primary energy of all organisms. To accomplish this task, plants respond to gravity for controlling their growth and development under terrestrial environment. For example, roots and stems display gravitropism. In addition, graviresponse has been implicated to be involved with circumnutation in plants. Another example of gravimorphogenesis is seen in the seedlings of cucurbitaceous plants; namely, unilateral positioning of a peg on the transition zone between the root and the hypocotyl. Plants also respond to various environmental cues, which interacts with graviresponse. However, mechanisms underlying the graviresponse and its relationship to other growth and development are not fully understood. Members of our working group have attempted to reveal the molecular mechanisms that essentially function in graviperception system and its involvement in various aspects of plant growth and development. We will further develop our research on the molecular dissections for obtaining general principles underlying the graviperception and its role in plant growth and development. Then, we will establish a model for spaceflight experiments to verify the hypothesis.Meeting sponsors: The Science Council of Japan, The Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (ISAS)(JAXA)

  40. Isolation of novel root gravitropic mutants of Arabidopsis using an interaction between gravitropism and phototropism

    N Fujii, Y Kanno, Y Miyazawa, H Takahashi

    PLANT AND CELL PHYSIOLOGY　47　S120-S120　2006

    ISSN： 0032-0781

  41. 根の重力屈性および水分屈性とオーキシンの輸送・分布

    藤井 伸治, 堀田 拓哉, KIM Dai-Hee, 宮沢 豊, KIM Kyung-Min, 高橋 秀幸

    根の研究 = Root research　14　(4)　176-176　2005/12/20

    ISSN： 0919-2182

  42. シロイヌナズナを用いた根の重力屈性と水分屈性の相互作用機作

    宮沢 豊, 根岸 洋, 金安 智子, 藤井 伸治, 高橋 秀幸

    根の研究 = Root research　14　(4)　2005/12/20

    ISSN： 0919-2182

  43. 5. Identification of molecules that dissect intimate relationship between gravitropism and hydrotropism

    Kaneyasu Tomoko, Kobayashi Akie, Takahashi Akiko, Miyazawa Yutaka, Fujii Nobuharu, Takahashi Hideyuki

    (40)　24-24　2005/10/13

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

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    Root systems are responsible for acquisition of all the water and nutrients from the soil. Therefore, it is important for plants to develop root systems for avoiding stressful environments and establishing their stand. Thus, roots have been shown to recognize many environmental signals that regulate root system architecture; namely, gravity, light, touch, and moisture gradients. Hydrotropism is the response of roots to a moisture gradient and thought to play an impotant role in avoiding water stress. However, only gravitropic response has been extensively studied among these responses, for gravitropism generally dominates other tropisms. In fact, gravitropic interference with hydrotropism has been shown in several plant species. At the same time, hydrotropism and gravitropism have been proved to share common features. But, how different stimuli lead to a common response remains unclear. To gain new insights on the problem, we searched the molecules that affect either or both gravitropism and hydrotropism. We treated Arabidopsis seedlings with various inhibitors and examined their effects on gravitropism and hydrotropism in roots. The results showed that inhibitors of polar auxin transport inhibited only gravitropism but not hydrotropism, while Ca^<2+> chelator was inhibitory to both tropisms. These results suggest that Ca^<2+> is essential for both gravitropism and hydrotropism, whereas polar auxin transport is necessary only for gravitropic response.

  44. 3. The role of auxin efflux carriers in gravimorphogenesis of cucumber seedlings

    Fujii Nobuharu, Kamada Motoshi, Hotta Takuya, Kim Dai Hee, Miyazawa Yutaka, Kim Kyung Min, Takahashi Hideyuki

    (40)　22-22　2005/10/13

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

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    Plant response to gravity is an important adaptive strategy to survive their environments. We have investigated the mechanism of the gravity response using cucumber seedlings. When cucumber seeds germinate in a horizontal position, a protuberance called a peg forms on the lower side of the transition zone between the root and the hypocotyl. The peg helps the cotyledons and the plumule to pull themselves out from the seed coat. In space-flight experiments, we found that cucumber seedlings grown in microgravity developed a peg on each side of the transition zone. This result suggests that gravity response results in the suppression of peg formation on the upper side of transition zone on Earth. It has been shown that auxin induces peg formation. When cucumber seedlings were treated with inhibitors of auxin efflux carrier, a peg was formed not only on the lower side but also on the upper side of the transition zone, suggesting that activation of auxin efflux carriers is required for the suppression of peg formation. To identify auxin efflux carriers involved in suppression of peg formation by graviresponse, we isolated 6 cucumber cDNAs of PIN auxin efflux carrier genes and investigated their mRNA accumulation by in situ hybridization. Our results showed that mRNAs of CsPIN1 and CsPIN6 accumulate in several tissues including endodermis postulated to be gravisensing cells, suggesting that CsPIN1 and CsPIN6 could play a role in the gravity-response of the transition zone.

  45. Development of experimental system for isolating novel Arabidopsis mutants of root gravitropism

    KANNO Yuji, FUJII Nobuharu, MIYAZAWA Yutaka, TAKAHASHI Hideyuki

    Biological sciences in space　19　(2)　95-97　2005/06/01

    Publisher: 日本宇宙生物科学会

    ISSN： 0914-9201

  46. Construction of an experimental system for separating hydrotropism from gravitropism in space

    MIYAZAWA Yutaka, KAKIMOTO Yoko, KANEYASU Tomoko, NAKAMURA Kazuko, FUJII Nobuharu, TAKAHASHI Hideyuki

    19　(2)　107-108　2005/06/01

    ISSN： 0914-9201

  47. Functional analysis of transcriptional regulation of cucumber CsARFs and CsIAAs

    Y Saito, D Kitazawa, Y Miyazawa, N Fujii, H Takahashi

    PLANT AND CELL PHYSIOLOGY　46　S115-S115　2005

    ISSN： 0032-0781

  48. Role of gibberellin-induced cell division in first internode elongation of a wheat variety tolerant to deep-sowing

    M Araki, L Chen, Y Miyazawa, N Fujii, H Suge, K Takeda, A Higashitani, H Takahashi

    PLANT AND CELL PHYSIOLOGY　46　S211-S211　2005

    ISSN： 0032-0781

  49. Tissue specific expression of cucumber PIN auxin efflux carrier genes

    N Fujii, T Hotta, DH Kim, M Kamada, Y Miyazawa, KM Kim, H Takahashi

    PLANT AND CELL PHYSIOLOGY　46　S115-S115　2005

    ISSN： 0032-0781

  50. 微小重力下における根の水分屈性とオーキシン動態 Invited

    高橋秀幸, 藤井伸治, 宮沢豊

    生物工学　83　560-564　2005

  51. Hormonal regulation of sex expression in plants

    S Yamasaki, N Fujii, H Takahashi

    PLANT HORMONES　72　79-110　2005

    DOI： 10.1016/S0083-6729(05)72003-3 　

    ISSN： 0083-6729

  52. Isolation and expression of a novel gravity-responsive gene of cucumber

    M Shimizu, K Suzuki, N Fujii, H Takahashi

    PLANT AND CELL PHYSIOLOGY　45　S183-S183　2004

    ISSN： 0032-0781

  53. Novel hydrotropic mutants of Arabidopsis roots: Hydrotropism, gravitropism, phototropism and waving response of rhy4 and rhy5

    A Takahashi, A Kobayashi, Y Kakimoto, N Fujii, H Takahashi

    PLANT AND CELL PHYSIOLOGY　45　S183-S183　2004

    ISSN： 0032-0781

  54. シロイヌナズナにおける水分屈性突然変異体(rhy1, rhy2, rhy3)の特性解析と突然変異遺伝子のマッピング

    小林 啓恵, 柿本 洋子, 藤井 伸治, 高橋 秀幸

    根の研究 = Root research　12　(4)　2003/12/19

    ISSN： 0919-2182

  55. シロイヌナズナの水分屈性突然変異体(rhy4, rhy5)の特性解析

    高橋 あき子, 小林 啓恵, 柿本 洋子, 藤井 伸治, 高橋 秀幸

    根の研究 = Root research　12　(4)　2003/12/19

    ISSN： 0919-2182

  56. Novel hydrotropism mutants of Arabidopsis thaliana and their altered waving response and phototropism

    TAKAHASHI Akiko, KOBAYASHI Akie, KAKIMOTO Yoko, FUJII Nobuharu, TAKAHASHI Hideyuki

    17　(3)　209-210　2003/10/01

    ISSN： 0914-9201

  57. The effect of light on the gravimorphogenesis of cucumber seedlings

    SAITO Yuko, FUJII Nobuharu, TAKAHASHI Hideyuki

    17　(3)　181-182　2003/10/01

    ISSN： 0914-9201

  58. Identification of the cDNA of differentially expressed genes in the transition zone of cucumber seedlings by fluorescent differential display

    SHIMIZU Minobu, SUZUKI Keita, FUJII Nobuharu, TAKAHASHI Hideyuki

    17　(3)　232-233　2003/10/01

    ISSN： 0914-9201

  59. Physiological and genetic characterization of hydrotropic mutants of Arabidopsis thaliana

    KOBAYASHI Akie, KAKIMOTO Yoko, FUJII Nobuharu, TAKAHASHI Hideyuki

    17　(3)　243-244　2003/10/01

    ISSN： 0914-9201

  60. Isolation of auxin efflux carrier cDNA from cucumber

    HOTTA Takuya, KIM Dai-Hee, KAMADA Motoshi, FUJII Nobuharu, KIM Kyung-Min, TAKAHASHI Hideyuki

    17　(3)　230-231　2003/10/01

    ISSN： 0914-9201

  61. Auxin-mediated response of cucumber seedlings to gravity

    Fujii N, Takahashi H

    Biological Sciences in Space　17　(2)　126-134　2003/08

    Publisher: 日本宇宙生物科学会

    DOI： 10.2187/bss.17.126 　

    ISSN： 0914-9201

  62. Gravity-induced asymmetry of localization of auxin-carrier proteins (CS-AUX1 and CS-PIN1) for gravimorphogenesis in cucumber seedlings

    M Kamada, N Fujii, A Higashitan, H Takahashi

    PLANT AND CELL PHYSIOLOGY　44　S197-S197　2003

    ISSN： 0032-0781

  63. Localization of CS-ARF mRNAs and CS-IAA rnRNAs in cucumber seedlings at the time of peg formation

    Y Saito, N Fujii, H Takahashi

    PLANT AND CELL PHYSIOLOGY　44　S197-S197　2003

    ISSN： 0032-0781

  64. Expression of CS-ARF genes at the stage of gravimorphogenesis in cucumber seedlings

    SAITO Yuko, FUJII Nobuharu, TAKAHASHI Hideyuki

    16　(3)　187-188　2002/11/01

    ISSN： 0914-9201

  65. Isolation and characterization of hydrotropic mutants in seedling roots of Arabidopsis thaliana

    KOBAYASHI Akie, KAKIMOTO Yoko, YAMAZAKI Yutaka, FUJII Nobuharu, TAKAHASHI Hideyuki

    16　(3)　151-152　2002/11/01

    ISSN： 0914-9201

  66. The effect of ethylene on the gravity-regulated formation of peg in cucumber seedlings

    SAITO Yuko, FUJII Nobuharu, TAKAHASHI Hideyuki

    16　(3)　185-186　2002/11/01

    ISSN： 0914-9201

  67. Determination of the developmental stage of flower buds for ethylene action on the sexual expression and expression analysis of ethylene receptor-related genes and ACC synthase gene in the flower buds of cucumber plants

    S Yamasaki, N Fujii, H Takahashi

    PLANT AND CELL PHYSIOLOGY　43　S134-S134　2002

    ISSN： 0032-0781

  68. Ethylene evolution and the responses to ethylene in a potential hermaphroditic cucumber plant, Tiv-1

    R Sawada, S Yamasaki, N Fujii, H Mizuno, S Matsuura, H Takahashi

    PLANT AND CELL PHYSIOLOGY　43　S134-S134　2002

    ISSN： 0032-0781

  69. Gravity-induced modification of auxin transport and distribution for pea formation in cucumber seedlings

    M Kamada, N Fujii, A Higashitani, H Takahashi

    PLANT AND CELL PHYSIOLOGY　43　S59-S59　2002

    ISSN： 0032-0781

  70. Roles of auxin and ethylene in peg formation of cucumber seedlings

    Y Saito, S Yamasaki, N Fujii, H Takahashi

    PLANT AND CELL PHYSIOLOGY　43　S59-S59　2002

    ISSN： 0032-0781

  71. Hydrotropism and differential expression of auxin-inducible genes in roots of cucumber seedlings

    KOBAYASHI Akie, MIZUNO Hidetoshi, FUJII Nobuharu, YAMASHITA Masamichi, TAKAHASHI Hideyuki

    15　(3)　246-247　2001/11

    ISSN： 0914-9201

  72. THE DAY LENGTH- AND ETHYLENE-CONTROLLED SEX EXPRESSION OF CUCUMBER FLOWERS

    Yamasaki Seiji, Fujii Nobuharu, Takahashi Hideyuki

    (36)　89-90　2001/10/09

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

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    Sex expression of cucumber plants is affected by the day length and a plant hormone, ethylene, Short-day conditions or the treatment with ethylene induce the femaleness whereas long-day conditions or the treatments with inhibitors of ethylene biosynthesis induce the maleness. In this study, we examined the correlation between the effects of day length and ethylene on sex expression of monoecious cucumber plants. The application of ethephone, an ethylene releasing agent, to the shoot apices of cucumber plants, when the leaf blade of the fourth leaf was approximately 2-cm long (defined as the 4-leaf stage), induced femaleness under the long-day (16h) condition. Also, the application of aminoethoxyvinyl glycine (AVG), an inhibitor of ethylene biosynthesis, to the shoot apices of cucumber plants at the 4-leaf stage induced maleness under the short-day (8h) condition. Ethephone treatment did not induce femalehess significantly under the short-day condition. Also, the cucumber plants grown under the short-day condition produced more ethylene than those grown under the long-day condition at the 4-leaf stage. Ethylene production from the shoot apices of cucumber plants and the expression of CS-ACS2 and CS-ERS showed circadian rhythm with a peak in the middle of the photoperiod. These results indicate that the femaleness of the cucumber flowers under the short-day condition mediate ethylene production. CS-ACS2 and CS-ERS genes may contribute to this ethylene signaling cascade in the femaleness of cucumber plants.

  73. Control of gravimorphogenesis by auxin: accumulation pattern of CS-IAA1 mRNA in cucumber seedlings grown in space and on the ground

    KAMADA Motoshi, FUJII Nobuharu, AIZAWA Sachiko, KAMIGAICHI Shigeki, MUKAI Chiaki, SHIMAZU Toru, TAKAHASHI Hideyuki

    Biological sciences in space　15　s239-s247　2001/10

    Publisher: 日本宇宙生物科学会

    ISSN： 0914-9201

  74. Morphogenesis in cucumber seedlings is negatively controlled by gravity

    TAKAHASHI Hideyuki, KAMADA Motoshi, YAMAZAKI Yutaka, FUJII Nobuharu, HIGASHITANI Atsushi, AIZAWA Sachiko, YOSHIZAKI Izumi, KAMIGAICHI Shigeki, MUKAI Chiaki, SHIMAZU Toru, FUKUI Keiji

    Biological sciences in space　15　s235-s238　2001/10

    Publisher: 日本宇宙生物科学会

    ISSN： 0914-9201

  75. THE GRAVITY-REGULATED FORMATION OF PEG IN CUCUMBER SEEDLINGS AND EXPRESSION OF AUXIN TANSPORTER GENES :

    KAMADA Motoshi, FUJII Nobuharu, HIGASHITANI Athushi, TAKAHASHI Hideyuki

    Plant and cell physiology　42　s112　2001

    Publisher: Japanese Society of Plant Physiologists

    ISSN： 0032-0781

  76. THE LIGHT INHIBITS THE PEG FORMATION IN CUCUMBER PLANTS :

    FUJII Nobuharu, KAMADA Motoshi, TAKAHASHI Hideyuki

    Plant and cell physiology　42　s112　2001

    Publisher: Japanese Society of Plant Physiologists

    ISSN： 0032-0781

  77. 根の屈性と植物ホルモン

    高橋 秀幸, 水野 英俊, 高橋 信行, 鎌田 源司, 藤井 伸治

    根の研究 = Root research　9　(4)　2000/12/20

    ISSN： 0919-2182

  78. The light effect on gravity-regulated morphogenesis in cucumber seedlings

    FUJII Nobuharu, KAMADA Motoshi, TAKAHASHI Hideyuki

    14　(3)　166-167　2000/10

    ISSN： 0914-9201

  79. 微小重力下における根の水分屈性

    高橋 秀幸, 水野 英俊, 鎌田 源司, 藤井 伸治, 東谷 篤志, 相澤 幸子, 上垣内 茂樹, 向井 千秋, 嶋津 徹

    根の研究 = Root research　9　(2)　2000/06/22

    ISSN： 0919-2182

  80. キュウリを用いた重力応答におけるオーキシンを介する転写制御機構の解析 (ライフサイエンス計画研究 地球型生命の原理の特殊性と普遍性) -- (植物の重力適応)

    高橋 秀幸, 藤井 伸治

    宇宙基地利用基礎実験費研究成果報告書　(1999)　171-174　2000/04

    Publisher: 宇宙科学研究所

  81. Gravimorphogenesis of Cucurbitaceae plants. Development of peg cells and graviperception mechanism in cucumber seedlings.

    Takahashi Hideyuki, Mizuno Hidetoshi, Kaneko Yasuko, Murata Takashi, Kamigaichi Shigeki, Aizawa Sachiko, Yoshizaki Izumi, Shimazu Toru, Fukui Keiji, Fujii Nobuharu, Kamada Motoshi, Higashitani Atsushi, Yamazaki Yutaka, Kobayashi Akie, Takano Mamoru, Yamasaki Seiji, Sakata Tadashi

    Biological Sciences in Space　14　(2)　64-74　2000

    Publisher: Japanese Society for Biological Sciences in Space

    DOI： 10.2187/bss.14.64 　

    ISSN： 0914-9201

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    We examined the effect of microgravity on the peg formation of cucumber seedlings for clarifying the mechanism of gravimorphogenesis in cucurbitaceous plants. The spaceflight experiments verified that gravity controls the formation of peg, hypocotyl hook and growth orientation of cucumber seedlings. Space-grown cucumber developed a peg on each side of the transition zone of the hypocotyl and root, indicating that on the ground peg formation is regulated negatively by gravity (Takahashi et al. 2000). It was found that the auxin-regulated gene, CS-IAA1, was strongly expressed in the transition zone where peg develops (Fujii et al. 2000). In the seedlings grown horizontally on the ground, CS-IAA1 transcripts were much abundant on the lower side of the transition zone, but no such differential expression of CS-IAA1 was observed in the space-grown cucumber (Kamada et al. 2000). These results imply that gravity plays a role in peg formation through auxin redistribution. By the negative control, peg formation on the upper side of the transition zone in the horizontally growing seedlings might be suppressed due to a reduction in auxin concentration. The threshold theory of auxin concentration accounted for the new concept, negative control of morphogenesis by gravity (Kamada et al. 2000). Anatomical studies have shown that there exists the target cells destined to be a peg and distinguishable at the early stage of the growth. Ultra-structural analysis suggested that endoplasmic reticulum develops well in the cells of the future peg. Furthermore, it was found that re-organization of cortical microtubules is required for the change in cell growth polarity in the process of peg formation. The spaceflight experiment with cucumber seedlings also suggested that in microgravity positive hydrotropic response of roots occurred without interference by gravitropic response (Takahashi et al. 1999b). Thus, this spaceflight experiment together with the ground-based studies has shown that cucumber seedling is an ideal for the study of gravimorphogenesis, hydrotropism and their interaction. Although peg formation is seen specifically in cucurbitaceous seedlings, it involves graviperception, auxin transport and redistribution and cytoskeletal modification for controlling cell growth polarity. This system could be a useful model for studying important current issues in plant biology.

  82. Microtubule reorganization in response to gravistimulation during cucumber peg development

    MURATA Takashi, KOBAYASHI Mayumi, FUJII Nobuharu, HIGASHITANI Atsushi, AIZAWA Sachiko, KAMIGAICHI Shigeki, SHIMAZU Toru, FUKUI Keiji, TAKAHASHI Hideyuki

    宇宙生物科学 = Biological sciences in space　13　(3)　282-283　1999/09

    ISSN： 0914-9201

  83. Negative control by gravity for the formation of peg in cucurbitaceous seedlings: Expession of the auixn-regulated genes in cucumber seedlings grown on the ground and under microgravity conditions

    KAMADA Motoshi, FUJII Nobuharu, HIGASHITANI Athushi, AIZAWA Sachiko, KAMIGAICHI Shigeki, SHIMAZU Toru, FUKUI Keiji, TAKAHASHI Hideyuki

    宇宙生物科学 = Biological sciences in space　13　(3)　280-281　1999/09

    ISSN： 0914-9201

  84. INVOVEMENT OF ENDO-XYLOGLUCAN TRANSFERASE (EXGT) GENE DURING THE DIFFERENTAL GROWTH OF PER(Pisum sativum L. ) ROOTS

    TAKANO Mamoru, FUJII Nobuharu, HIGASHITANI Atushi, HIRASAWA Tadashi, NISHITANI Kazuhiko, TAKAHASHI Hideyuki

    40　s146-s146　1999/03

    ISSN： 0032-0781

  85. DEVELOPMENTAL PATTERN OF PEG IN CUCUMBER SEEDLINGS AND ITS NEGATIVE CONTROL BY GRAVITY

    TAKAHASHI Hideyuki, FUJII Nobuharu, KAMADA Motoshi, TAKANO Mamoru, YAMAZAKI Yutaka, HIGASHITANI Atsushi, KANEKO Yasuko, KAMIGAICHI Shigeki, AIZAWA Sachiko, YOSHIZAKI Izumi, SHIMAZU Toru, FUKUI Keiji

    40　s144-s144　1999/03

    ISSN： 0032-0781

  86. Mechanism of the differential growth in hydrotropically responding pea roots

    TAKANO Mamoru, FUJII Nobuharu, HIGASHITANI Atushi, HIRASAWA Tadashi, NISHITANI Kazuhiko, TAKAHASHI Hideyuki

    7　(4)　126-126　1998/12/16

    ISSN： 0919-2182

  87. キュウリの花芽の雌雄性分化におけるエチレンレセプター様遺伝子群の発現解析

    山崎 聖司, 藤井 伸治, 高橋 秀幸

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan　62　135-135　1998/09/01

  88. A ROLE OF CYTOSKELTAL STRUCTURE OF CORTICAL CELLS IN PEG FORMATION OF CUCUMBER SEEDLINGS

    KOBAYASHI Mayumi, MURATA Takashi, FUJII Nobuharu, HIGASHITANI Atsushi, TAKAHASHI Hideyuki

    39　S90-S90　1998/05

    ISSN： 0032-0781

  89. GENE EXPRESSION IN THE GRAVITY-REGULATED MORPHOGENESIS OF CUCUMBER SEEDLINGS

    FUJII Nobuharu, TAKAHASHI Hideyuki

    39　S89-S89　1998/05

    ISSN： 0032-0781

  90. ウリ科植物の重力形態形成-キュウリ芽ばえのペグ形成機構について

    高橋 秀幸, 藤井 伸治, 菅 洋

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan　61　65-65　1997/09

  91. キュウリ由来オーキシン制御遺伝子とエチレン受容体(ETR1)類似遺伝子の構造解析

    藤井 伸治, 高橋 秀幸

    宇宙生物科学 = Biological sciences in space　11　(3)　246-247　1997/09

    ISSN： 0914-9201

  92. 微小重力下における根の姿勢制御関連遺伝子の発現

    高野 守, 藤井 伸治, 東谷 篤志, 高橋 秀幸, 西谷 和彦, 平沢 正, 山下 雅道

    宇宙生物科学 = Biological sciences in space　11　(3)　252-252　1997/09

    ISSN： 0914-9201

  93. Possible relationship between the expression of EXGT gene and differential growth in hydrotropically responding roots of ageotropum pea

    M Takano, N Fujii, K Nishitani, T Hirasawa, H Takahashi

    PLANT PHYSIOLOGY　114　(3)　64-64　1997/07

    ISSN： 0032-0889

  94. Expression of ETR1-like genes during gravity-regulated morphogenesis in cucumber seedlings.

    N Fujii, H Takahashi

    PLANT PHYSIOLOGY　114　(3)　619-619　1997/07

    ISSN： 0032-0889

  95. EXPRESSION OF ETR1-LIKE GENES DURING GRAVITY-REGULATED MORPHOGENESIS IN CUCUMBER SEEDLINGS

    FUJII Nobuharu, TAKAHASHI Hideyuki

    38　s79　1997/03

    ISSN： 0032-0781

  96. EXPRESSION OF ENDO-XYLOGLUCAN TRANSFERASE (EXGT) GENE DURING THE HYDROTROPIC RESPONSE OF PEA(Pisum sativum L.) ROOTS

    TAKANO Mamoru, FUJII Nobuharu, NISHITANI Kazuhiko, HIRASAWA Tadashi, TAKAHASHI Hideyuki

    Plant Cell Physiol.　38　s110　1997/03

    ISSN： 0032-0781

  97. キュウリ芽生えの重力形態形成における遺伝子発現の解析

    藤井 伸治, 高橋 秀幸, 菅 洋

    宇宙生物科学 = Biological sciences in space　10　(3)　216-217　1996/10

    ISSN： 0914-9201

  98. 突然変異体を利用したオオムギ芽生えの重力反応の解析

    阿部 清美, 藤井 伸治, 高橋 秀幸, 菅 洋

    宇宙生物科学 = Biological sciences in space　10　(3)　172-173　1996/10

    ISSN： 0914-9201

  99. 疑似微小重力下における根の水分屈性の誘導

    高野 守, 藤井 伸治, 高橋 秀幸, 菅 洋, 山下 雅道

    宇宙生物科学 = Biological sciences in space　9　(3)　190-191　1995/09

    ISSN： 0914-9201

  100. 重力応答を欠失したオオムギの突然変異体(serpentina)を用いた重力応答遺伝子単離の試み

    藤井 伸治, 高橋 秀幸, 菅 洋

    宇宙生物科学 = Biological sciences in space　9　(3)　188-189　1995/09

    ISSN： 0914-9201

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Presentations 20

  1. 微小重力環境がキュウリ芽生えの発生生理に与える影響

    藤井伸治, 山崎千秋, 宮沢豊, 鎌田源司, 笠原春夫, 長田郁子, 嶋津徹, 伏島康男, 東端晃, 山崎丘, 石岡憲昭, 高橋秀幸

    第31回宇宙環境利用シンポジウム　2017/01/16

  2. A Pathway that Laterally Transports Auxin from the Upper Side to the Lower Side of the Transition Zone of Cucumber Seedlings via Endodermal Layers is Formed Due to Gravitstimulation International-presentation

    Nobuharu Fujii, Chiaki Yamazaki, Yutaka Miyazawa, Motoshi Kamada, Haruo Kasahara, Ikuko Osada, Toru Shimazu, Yasuo Fusejima, Akira Higashibata, Takashi Yamazaki, Noriaki Ishioka, Hideyuki Takahashi

    11th Asian Microgravity Symposium　2016/10/25

  3. キュウリの根の重力屈性による水分屈性の抑制機構

    日本宇宙生物科学会第30回大会　2016/10/13

  4. キュウリの根の水分屈性と重力屈性に関わる遺伝子の網羅的発現解析

    藤井伸治, 宮林彩智子, 小林啓恵, 山崎千秋, 宮沢豊, 鎌田源司, 笠原春夫, 長田郁子, 嶋津徹, 伏島康男, 東端晃, 山崎丘, 石岡憲昭, 高橋秀幸

    第30回宇宙環境利用シンポジウム　2016/01/19

  5. キュウリの根の重力屈性時と水分屈性時に偏差的に発現する遺伝子の比較

    宮林彩智子, 藤井伸治, 小林啓恵, 山崎千秋, 宮沢豊, 鎌田源司, 笠原春夫, 長田郁子, 嶋津徹, 伏島康男, 東端晃, 山崎丘, 石岡憲昭, 高橋秀幸

    日本宇宙生物科学会第29回大会　2015/09/26

  6. 陸上植物の水獲得に機能する根の水応答機構の解明

    藤井伸治, 宮林彩智子, 中島祐介, 奈良敬孝, 諸橋恵太, 小林啓恵, 宮沢豊, 高橋秀幸

    文部科学省科学研究費新学術領域研究「ゲノム支援」拡大班会議　2015/09/06

  7. 過重力条件を利用したシロイヌナズナのarg1 pgm二重突然変異体の重力屈性を低下させる突然変異 (enhancer of arg1 pgm) の単離

    藤井伸治, 山川あゆみ, 菅村裕子, 宮沢豊, 山下雅道, 高橋秀幸

    第56回日本植物生理学会年会　2015/03/16

  8. キュウリ芽生えの重力形態形成の制御機構

    藤井伸治, 山崎千秋, 宮沢豊, 鎌田源司, 笠原春夫, 長田郁子, 嶋津 徹, 伏島康男, 東端晃, 山崎丘, 石岡憲昭, 高橋秀幸

    第29回宇宙環境利用シンポジウム　2015/01/24

  9. シロイヌナズナのarg1 pgm二重突然変異体に残存した重力屈性を低下させるenhancer of arg1 pgm突然変異体の単離

    藤井伸治, 山川あゆみ, 菅村裕子, 宮沢豊, 山下雅道, 高橋秀幸

    東北植物学会第4回大会　2014/12/13

  10. シロイヌナズナのarg1 pgm二重突然変異体の重力屈性を低下させる突然変異の単離

    藤井伸治, 山川あゆみ, 菅村裕子, 宮沢豊, 山下雅道, 高橋秀幸

    日本宇宙生物科学会第28回大会　2014/09/22

  11. キュウリ内皮細胞における CsPIN1 オーキシン排出キャリアの局在変化の重力応答性

    藤井伸治, 山崎千秋, 宮沢豊, 鎌田源司, 笠原春夫, 長田郁子, 嶋津徹, 伏島康男, 東端晃, 山崎丘, 石岡憲昭, 高橋秀幸

    東北植物学会第3回大会　2013/12/14

  12. シロイヌナズナのpgm arg1二重突然変異体を利用した新規重力屈性突然変異の単離の試み

    藤井伸治, 山川あゆみ, 宮沢豊, 山下雅道, 高橋秀幸

    日本宇宙生物科学会第27回大会　2013/09/27

  13. Graviresponse Induces Re-Localization of an Auxin Efflux Carrier, CsPIN1, during Gravimorphogenesis of Cucumber Seedlings International-presentation

    Nobuharu Fujii, Chiaki Yamazaki, Yutaka Miyazawa, Motoshi Kamada, Haruo Kasahara, Ikuko Osada, Toru Shimazu, Yasuo Fusejima, Akira Higashibata, Takashi Yamazaki, Noriaki Ishioka, Hideyuki Takahashi

    34th Annual Meeting International Gravitational Physiology　2013/06/23

  14. キュウリ芽ばえの内皮でのオーキ シン排出キャリアCsPIN1タンパク質局在パターンの重力応答性

    藤井伸治, 矢内健一, 堀田拓哉, 宮沢豊, 高橋秀幸

    第48回日本植物生理学会年会　2007/03/28

  15. キュウリ芽ばえの内皮におけるオーキシン排出キャリアCsPIN1タンパク質の重力応答性

    藤井伸治, 矢内健一, 堀田拓哉, 宮沢豊, 高橋秀幸

    宇宙利用シンポジウム (第23回)　2007/01/15

  16. シロイヌナズナの根における重力屈性と水分屈性の干渉作用

    藤井伸治, 中村和子, 宮沢豊, 高橋秀幸

    日本宇宙生物科学会第20回大会　2006/09/28

  17. キュウリのPIN(/)- オーキシン排出キャリア遺伝子の組織特異的発現

    堀田拓哉, Dai-Hee Kim, 鎌田源司, 宮沢豊, Kyung-Min Kim, 高橋秀幸

    日本植物生理学会 第46 回日本植物生理学会年会　2005/03/24

  18. キュウリのオーキシン排出キャリア遺伝子群cDNAの単離と発現解析

    堀田拓哉, Dai-Hee Kim, 鎌田源司, 宮沢豊, Kyung-Min Kim, 高橋秀幸

    宇宙利用シンポジウム (第21回)　2005/01/12

  19. 重力に対する植物の応答機構

    日本宇宙生物科学会第18回大会　2004/09/30

  20. キュウリのオーキシン排出キャリア遺伝子群の発現解析

    堀田拓哉, Dai-Hee Kim, 鎌田源司, Kyung-Min Kim, 高橋秀幸

    日本宇宙生物科学会第18回大会　2004/09/30

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Research Projects 15

  1. Auxin-mediated gravity response in plants Competitive

    System: Funded Research

    1995/04 - Present

  2. Identification and analysis of genetic variants that enhance the expression of gravitropism in Arabidopsis roots

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2023/04/01 - 2026/03/31

  3. The role of genetic variations in peptide hormone genes in the adaptive evolution of Arabidopsis

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Transformative Research Areas (A)

    Institution: Tohoku University

    2023/04/01 - 2025/03/31

  4. Effects of differences of properties of root hydrotropism on root system development and plant growth

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

  5. Does gene flow along environmental gradient inhibit adaptation? An approach using the optimality theory

    Hikosaka Kouki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

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    In different environments, selective pressures in each environment enhance adaptive differentiation, but gene flows from the different environment may inhibit evolution. Here, we tested whether gene flow along altitude inhibit adaptation to the respective altitude in Arabidopsis halleri. The growth analysis with optimization theory reveled that highland ecotype adapts to higher nitrogen conditions while lowland ecotype adapts to a wide range of nitrogen conditions. Genetic analysis showed that gene flow occurs but in some single nucleotide polymorphisms (SNP) there was a clear differentiation in allele frequency along altitude, probably as a result of strong selection. We conclude genetic differentiation occurs if environmental condition is very different between two subpopulations even when their distance was small.

  6. Analysis of a gravity-sensing pathway using Arabidopsis multiple mutants

    FUJII Nobuharu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2019/03/31

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    It has been believed that LAZY1 proteins play an important role on sense of gravity in plants. However, the molecular functions of LAZY1 proteins have not been revealed. In barley, agravitropic serpentina mutant were isolated by screening 600,000 M3 plants mutagenized by gamma ray. In this study, a missense mutation in HvLAZY1 gene was found in serpentina mutant. Furthermore, the linkage analysis using by 78 F2 plants that were crossed between WT (Chikurin-ibaraki No. 1) and serpentina mutant indicated that the missense mutation cosegregated with agravitropisc phenotype, suggesting a possibility that the missense mutation caused serpentina mutation.

  7. Analysis of pathways of gravity-sensing and gravity-signaling in plants

    FUJII NOBUHARU

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    Sensing of gravity in plants is composed of amyloplast sedimentation (PGM pathway) and ARG1 pathway. However, a gravity-sensing molecule that converts gravistimulation into intracellular signals has not been identified. In this study, we found that gravity-responses in arg1 pgm double mutants could be detected under the hypergravity condition that was created by a low-speed centrifuge. Therefore, we screened M2 seedlings of arg1 pgm double mutants that were mutagenized with EMS, and isolated 4 lines of enhancer of arg1 pgm mutants that exhibited less gravity-responses than arg1 pgm mutants did. Hereafter, we expect that analysis of these mutants will allow us to obtain a new insight of how plants sense gravity.

  8. Mechanism of root hydrotropism for water acquisition in land plants

    TAKAHASHI Hideyuki, MIYAZAWA Yutaka, FUJII Nobuharu, MIYAZAWA Yutaka, YAMAZAKI Tomokazu, KOBAYASHI Akie, MORITA Miyo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Tohoku University

    2010/04/01 - 2015/03/31

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    We attempted to understand the functions of MIZ1 and MIZ2 molecules essential for hydrotropism in roots, an overall pathway of hydrotropism, and its role in nature. We identified cells/tissues required for hydrotropism and novel regulators of hydrotropism in relation to MIZ1 and MIZ2 functions in Arabidopsis roots. It was shown that light and abscisic acid independently regulated MIZ1 transcription and thereby hydrotropic response and that MIZ1 might play a role in hydrotropism and lateral root formation by negatively regulating auxin content. Furthermore, we found that MIZ1-overexpressors enhanced hydrotropic response and the MIZ1-mediated hydrotropism could contribute to plant biomass production and survival in nature by regulating root-system development under water-limited conditions. On the other hand, analyses with other plants such as rice, lotus, and peas revealed the molecular mechanisms that differed among plant species.

  9. Modeling of CO2 response of plant functions

    HIROSE Tadaki, HIKOSAKA Kouki, KINUGASA Toshihiko, FUJII Nobuharu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    2009/07/23 - 2014/03/31

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    44 ecotypes of Arabidopsis thaliana were grown at two CO2 levels. We found that there was a large variation in CO2 response of growth rates among the ecotypes. The variation in photosynthetic nitrogen use efficiency was the main cause of the variation in the growth rates. 5 pairs of deciduous and evergreen woody species were grown at two CO2 levels. We found that CO2 responses of growth and photosynthesis were not significantly different among deciduous and evergreen plants. We studied leaf and nitrogen dynamics in rice canopy grown under the field CO2 enrichment experiment. We found that nitrogen availability significantly affected leaf and nitrogen dynamics whereas elevated CO2 did not. We constructed a new model of leaf dynamics in a leaf canopy introducing a game theory. The model successfully predicted environmental response of canopy structure, including CO2 responses.

  10. Analysis of molecular mechanism for root hydrotropism that plays a role in plant adaptation to drought conditions

    TAKAHASHI Hideyuki, FUJII Nobuharu, MIYAZAWA Yutaka

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2008 - 2011

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    We attempted to reveal molecular mechanism of hydrotropism in Arabidopsis roots by analyzing the functions of MIZ1 and MIZ2 that play essential roles in hydrotropism. First, we generated the overexpressors of MIZ1 (MIZ1OEs)and analyzed their phenotypes. We found that MIZ1OEs showed an enhanced hydrotropism and a reduced lateral root formation. These phenomena likely resulted form the decreased level of auxin content in MIZ1OEs. Also, it was shown that the miz2 mutation is epistatic to MIZ1 overexpression. MIZ1-GFP introduced into miz1 mutant complemented the mutant phenotype and was detected at high levels in cortical cells and lateral root cap cells. MIZ1-GFP was fractionated into a soluble protein fraction and an endoplasmic reticulum (ER)membrane fraction, where it was bound to the surface of the ER membrane at the cytosolic side. Furthermore, we succeeded in isolating proteins that bind MIZ1 by immunoprecipitation method and LC-MS/MS analysis. In addition, we obtained mutants of suppressors of both miz1 and miz2. This study showed that light and ABA signaling are independently integrated into MIZ1 expression and regulate hydrotropism. Then, we demonstrated that the MIZ1-regulated hydrotropism of lateral roots plays an important role in root system development in soil and contributes to drought avoidance, which results in better survival and yield under the water-limited conditions.

  11. Molecular mechanism conferring function of gravity-responses to columella cells in root tip

    FUJII Nobuharu, MIYAZAWA Yutaka

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2007 - 2008

  12. Plant growth regulation useful for plant production in arid environment

    TAKAHASHI Hideyuki, FUJII Nobuharu, YUTAKA Miyazawa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2004 - 2006

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    We isolated seven Arabidopsis mutants (mizu-kussei : miz) defective in root hydrotropism. We characterized their phenotypes and identified genes responsible for the mutations of miz1 and miz6. Roots of both miz1 and miz6 showed impaired hydrotropism, but their gravitropism, elongation growth and morphological feature were normal. These results suggest that the pathway inducing hydrotropism is independent of gravitropism. MIZ1 phenotype resulted from a single recessive mutation, which encoded a novel protein containing a domain (the MIZ domain) that was highly conserved among terrestrial plants. We hypothesized that MIZ1 evolved to play an important role in adaptation to terrestrial life. In addition, a pMIZ1::GEIS fusion gene was strongly expressed in columella cells of the root cap but not in the elongation zone, suggesting that MIZ1 functions in the early phase of the hydrotropic response. We found that auxin response is indispensable for both hydrotropism and gravitropism, but mechanisms for auxin redistribution differ between hydrotropism and gravitropism in Arabidopsis roots. A variety of wheat, Hong Mang Mai, exhibits tolerance to deep-sowing conditions by extreme elongation of the first internodes in soil. To exert this response, the first internodes of Hong Mang Mai possessed greater ability to produce cells, compared to other varieties. It was revealed that gibberellin plays roles in both cell elongation and cell division of the internodes. However, extraordinary elongation of the first internodes of Hong Mang Mai occurred due to cell division rather than cell elongation. Based on these results, we are now studying molecular mechanisms for the deep-sowing tolerance of Hong Mang Mai. Plants respond to gravity for controlling their growth orientation. We found that gravisensing is required for circumnutation and winding response in morning glory and that glycine-rich protein (CsGRP1) and auxin-mediated transcription (CsARF2 and CsARF5) play roles in the gravity regulation of peg formation in cucumber seedlings.

  13. Adaptive strategies of plants to drought and high-temperature conditions and their control for plant cultivation in arid area

    TAKAHASHI Hideyuki, FUJII Nobuharu, HIGASHITANI Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2001 - 2003

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    To obtain fundamental knowledge on the adaptive strategies of plants and to develop new methods and plants that could be used for plant cultivation in arid area, we studied the mechanisms for deep-sowing tolerance of seedlings, root hydrotropism, and high-temperature-induced male sterility. Wheat seedlings emerge from the soil by elongating their first internodes. A variety of wheat tolerant to deep-sowing conditions was more sensitive to gibberellin (GA); the first internodes treated with GA elongated longer than 40 cm in the dark. Accordingly, the seedlings successfully emerged even from 40-cm depth in the soil. This GA response of the wheat was achieved by accompanying simultaneous uptake of potassium from the culture media for cellular osmoregulaiion and spiral growth for reducing mechanical impedance in the soil. We established an experimental system for the study of hydrotropism in Arabidopsis roots and revealed the involvement of abscisic acid, auxin and waving response with hydrotropism. In addition, we found that moisture gradient or water stress caused immediate degradation of the starch anchors, amyloplasts, in columella cells of Arabidopsis roots, which reduced the responsiveness of roots to gravity and thus allowed them to exhibit hydrotropism without interference by gravitropism. Also, we isolated unique hydrotropic mutants of Arabidopsis, which enabled us to dissect molecular mechanism for hydrotropism in seedling roots. We found that high temperature during floral organogenesis induced male sterility in barley. In relation to the male sterility, high temperature inhibited the expression of histone H3,H4 and glycine-rich RNA binding protein genes whose expressions are dramatically induced just before anther development. Furthermore, it was suggested that the early development and differentiation of barley anthers are hypersensitive to high temperature stress due to the inhibited transcriptions.

  14. Plant growth and cultivation under environmental stresses

    TAKAHASHI Hideyuki, FUJII Nobuharu, NISHIZAWA Takeaki, HIGASHITANI Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B).

    Institution: Tohoku University

    1997 - 2000

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    We have studied the mechanisms useful for the adaptation of plants to the conditions of environmental stresses. (1) We found a new gravimorphism of plants using agravitropic mutants of rice and barley. Furthermore, we discovered that gravimorphogenesis of cucurbitaceous seedlings was negatively controlled by gravity, which was accounted by the threshold theory of auxin concentration. By cloning and expression analysis of auxin-inducible genes and its transcriptional factor, we established a working hypothesis for the auxin regulation of the gravity's negative control. (2) A variety of wheat, Hong Mang Mai, was found to be tolerant to deep-seeding conditions in semi-arid area because it was super-sensitive to gibberellins in inducing strong elongation of the first internode. Surprisingly, the presence of potassium in culture media was absolutely required for the GA-induced elongation. (3) We established the experimental system for the study of hydrotropism in the roots of cucumber and Arabidopsis. It was found that auxin transport and distribution, calcium ion, abscisic acid and endo-xyloglucan transferase played important roles in root hydrotropism. (4) We established a model system for the study of the high-temperature-induced injury of the reproductive growth in barley. It was suggested that the expression of a calcium-dependent protein kinase was involved in the male sterility caused by high-temperature stress. We also discovered some genes that could be induced or suppressed in response to high temperature by adopting the serial analysis of gene expression. We have cloned several genes responsible for above responses of plants, which accounted, at least in part, for the mechanisms of plant adaptation to particular environment such as gravitational field and arid area. The accomplishments of the present study may lead to the development of new plants and regulatory methods of plant growth for improving crop yield.

  15. Sex determination of flower in cucumber plants Competitive

    1998/04 -

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Social Activities 1

  1. 清真学園高等学校・中学校

    2006/07/08 -

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    講演テーマ「中高生に伝える、生物学の楽しみ ～NASAでの研究から～」

Other 1

  1. キュウリの花の性分化の制御機構に関する研究

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    キュウリの花の性表現はF(/)-遺伝子およびM(/)-遺伝子の優劣により決定され、F-M-(/)-は雌性型、ffM-(/)-は混性型、F-mm(/)-は両性花型、ffmm(/)-は雄性両性同株型となる。F(/)-遺伝子産物はエチレン生合成を制御し、M(/)-遺伝子産物はその下流（エチレンシグナル伝達経路）において雄蕊の発育抑制を担うことにより、キュウリでは多彩な性表現が遺伝的に制御されうることを明らかにした。さらに、雌性型（F-M-(/)-）と混性型（ffM-(/)-）を比較し、F(/)-が機能を失うと、雌蕊の発育抑制と雄蕊の発育促進が一部の花芽で生じる（雄花化）ことから、F(/)-遺伝子産物は雌蕊の発育促進と雄蕊の発育抑制（雌花化）を一部の花芽で担うと仮定し、各品種におけるエチレン、F(/)-およびM(/)-遺伝子による性表現制御を推測した。遺伝型がff(/)-の場合、一部の花芽で雌性化に十分なエチレンが合成される一方で、他の花芽においては雌性化に十分なエチレンが合成されずに、遺伝型M-(/)-と組み合わさることにより混性型が生じると推測される。このF(/)-遺伝子産物の想定される機能は、遺伝型ffmm(/)-が雄性両性同株型となることと矛盾しない。すなわち、遺伝型mm(/)-であるため、全ての花芽において雄蕊の発育抑制が生じず、遺伝型ff(/)-であるため、一部の花芽で雌蕊の発育促進に十分なエチレンが合成され両性花となり、他の雌蕊の発育促進に十分なエチレンが合成されない花芽では雄花を着生すると推測される。