Details of the Researcher

PHOTO

Ryo Hamai
Section
Graduate School of Dentistry
Job title
Assistant Professor
Degree

  • 博士（工学）（九州工業大学）

  • 修士（工学）（九州工業大学）

e-Rad No.
00824004

Research History 2

  • 2018/04 - Present
    Tohoku University　Graduate School of Dentistry　Assistant Professor

  • 2017/04 - 2018/03
    Japan Society for the Promotion of Science

Education 4

  • Kyushu Institute of Technology　Graduate School of Life Science and Systems Engineering　Department of Life Science and Systems Engineering

    2015/04 - 2018/03

  • Kyushu Institute of Technology　Graduate School of Life Science and Systems Engineering　Biological Functions and Engineering

    2013/04 - 2015/03

  • National Institute of Technology, Toyama College

    2011/04 - 2013/03

  • 富山工業高等専門学校　環境材料工学科

    2006/04 - 2011/03

Committee Memberships 2

  • 日本バイオマテリアル学会　評議員

    2022 - Present

  • 日本歯科理工学会　代議員

    2020/04 - Present

Professional Memberships 3

  • THE JAPANESE SOCIETY FOR BIOMATERIALS

  • THE JAPANESE SOCIETY FOR DENTAL MATERIALS AND DEVICES

  • The Ceramic Society of Japan

Research Interests 2

  • 有機－無機複合体

  • 生体材料

Research Areas 1

  • Nanotechnology/Materials / Composite materials and interfaces /

Awards 5

  1. Japanese and Korean Biomaterials Societies Young Scientist Exchange Program Award (2022)

    2022/11　The Japanese Society for Biomaterials

  2. 特定セッション若手優秀発表賞

    2021/09　日本セラミックス協会第34回秋季シンポジウム 特定セッション「先進セラミックバイオマテリアルの新展開と次世代型材料機能設計指針の構築」

  3. 第73回春期学術講演会 企業賞（カボデンタルシステムズジャパン株式会社賞）

    2019/04　日本歯科理工学会

  4. Award for Encouragement of Research in IUMRS-ICAM 2017 Symposium D-5

    2017/11

  5. 特定セッション学生優秀発賞

    2016/09　日本セラミックス協会第29回秋季シンポジウム 特定セッション「生体との調和を生み出すセラミックスの開発と評価」　「分子構造の異なるリン酸基を含有する有機‐無機複合体のアパタイト形成能」

Papers 39

  1. C2C12 myoblast differentiation stimulated by octacalcium phosphate material Peer-reviewed

    Howard Tu-Shen CHEN, Yukari SHIWAKU, Ryo HAMAI, Kaori TSUCHIYA, Kensuke YAMAUCHI, Tetsu TAKAHASHI, Osamu SUZUKI

    Dental Materials Journal　44　(4)　446-455　2025/07/25

    Publisher: Japanese Society for Dental Materials and Devices

    DOI： 10.4012/dmj.2024-382 　

    ISSN： 0287-4547

    eISSN： 1881-1361

  2. Osteoclast formation in the co-presence of octacalcium phosphate and inorganic phosphorus-containing gelatin in vitro Peer-reviewed

    Ryo HAMAI, Kenya TOMITSUKA, Masahiro OKADA, Kaori TSUCHIYA, Tai KIMURA, Susumu SAKAI, Kensuke YAMAUCHI, Osamu SUZUKI

    Dental Materials Journal　44　(4)　414-426　2025/07/25

    Publisher: Japanese Society for Dental Materials and Devices

    DOI： 10.4012/dmj.2024-367 　

    ISSN： 0287-4547

    eISSN： 1881-1361

  3. Effect of octacalcium phosphate on the release behavior of osteogenic factors from PLGA/PCL microspheres Peer-reviewed

    Tai KIMURA, Ryo HAMAI, Masahiro OKADA, Kaori TSUCHIYA, Kensuke YAMAUCHI, Osamu SUZUKI

    Dental Materials Journal　44　(4)　386-393　2025/07/25

    Publisher: Japanese Society for Dental Materials and Devices

    DOI： 10.4012/dmj.2024-366 　

    ISSN： 0287-4547

    eISSN： 1881-1361

  4. An MSC Chemotactic and Bone-Replaceable PLGA Material Involving Chemokine Loading OCP for Orthopedic Bone Defect Repair Peer-reviewed

    Yasuaki Kuriyama, Ryo Hamai, Yu Mori, Ryuichi Kanabuchi, Kazuyoshi Baba, Kaori Tsuchiya, Toshimi Aizawa, Osamu Suzuki

    ACS Applied Bio Materials　8　(7)　5869-5882　2025/06/23

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsabm.5c00549 　

    ISSN： 2576-6422

    eISSN： 2576-6422

  5. Effect of Octacalcium Phosphate on Osteogenic Differentiation of Induced Pluripotent Stem Cells in a 3D Hybrid Spheroid Culture Peer-reviewed

    Yuki Sugai, Ryo Hamai, Yukari Shiwaku, Takahisa Anada, Kaori Tsuchiya, Tai Kimura, Manami Tadano, Kensuke Yamauchi, Tetsu Takahashi, Hiroshi Egusa, Osamu Suzuki

    Biomimetics　10　(4)　205　2025/03/26

    Publisher: MDPI AG

    DOI： 10.3390/biomimetics10040205 　

    eISSN： 2313-7673

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    Octacalcium phosphate (OCP) has been shown to exhibit an osteogenic property and, therefore, has been utilized recently as a bone substitute, clinically. However, the stimulatory capacity for induced pluripotent stem (iPS) cells is not known. This study investigated whether OCP enhances osteoblastic differentiation of three-dimensionally cultured spheroids of iPS cells compared to hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). Mouse iPS cells were mixed with smaller (less than 53 μm) or larger (300–500 μm) sizes of calcium phosphate (CaP) granules and cultured in a laboratory-developed oxygen-permeable culture chip under minimizing hypoxia for up to 21 days. Osteoblastic differentiation was estimated by the cellular alkaline phosphatase (ALP) activities. The degree of supersaturation (DS) with respect to CaP phases was determined from the media chemical compositions. Incubated CaP materials were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The culture promoted well the formation of hybrid spheroids of CaP materials and iPS cells regardless of the type of materials and their granule sizes. The ALP activity of OCP was about 1.5 times higher than that of β-TCP and HA in smaller granule sizes. FTIR, XRD, and DS analyses showed that larger OCP granules tended to hydrolyze to HA slightly faster than smaller granules with time while HA and β-TCP materials tended to remain unchanged. In conclusion, the results suggest that OCP enhances the osteogenic differentiation of iPS cells more than HA and β-TCP through a mechanism of hydrolyzing to HA. This inherent material property of OCP is essential for enhancing the osteoblastic differentiation of iPS cells.

  6. Enhanced osteogenic capacity of octacalcium phosphate involving adsorption of stromal-derived factor-1 in a standardized defect of a rat femur Peer-reviewed

    Ryuichi Kanabuchi, Ryo Hamai, Yu Mori, Soshi Hamada, Yukari Shiwaku, Yuko Sai, Kaori Tsuchiya, Toshimi Aizawa, Osamu Suzuki

    Journal of Materials Science: Materials in Medicine　36　(1)　23　2025/02/28

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10856-025-06872-9 　

    eISSN： 1573-4838

  7. Adsorption of Serum Fetuin onto Octacalcium Phosphate and Its Relation to Osteogenic Property Peer-reviewed

    Yuki Tsuboi, Ryo Hamai, Kyosuke Okuyama, Kaori Tsuchiya, Yukari Shiwaku, Kensuke Yamauchi, Osamu Suzuki

    International Journal of Molecular Sciences　26　(3)　1391　2025/02/06

    Publisher: MDPI AG

    DOI： 10.3390/ijms26031391 　

    eISSN： 1422-0067

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    This study aimed to investigate how the chemical elements in relation to octacalcium phosphate (OCP) hydrolysis affect the osteoblastic differentiation in the presence of serum fetuin. The adsorption of fetuin onto OCP was examined in buffers having different degrees of supersaturation (DS) with respect to OCP and hydroxyapatite (HA) at pH 7.4 and 37 °C. The osteoblastic differentiation of mesenchymal stem cells (MSCs) was evaluated in cultures with OCP and 0 to 0.8 mg/mL of fetuin. The amount of fetuin adsorbed increased with increasing DS in the buffer. In the MSC culture, the coexistence of OCP and 0.2–0.4 mg/mL of fetuin close to serum level increased alkaline phosphatase activity; however, the activity was suppressed by 0.2–0.8 mg/mL of fetuin. Transmission electron microscopy revealed de novo crystal formation on OCP in supersaturated buffer and culture media with respect to OCP and HA at lower fetuin concentrations. Infrared spectroscopy and DS estimation indicate that the hydrolysis of OCP with de novo apatite formation was promoted in the culture media at 0.2–0.4 mg/mL of fetuin. These results suggest that OCP may promote osteoblastic differentiation if the suitable conditions are attained regarding the chemical elements and fetuin adsorption around OCP.

  8. Enhancement of the Cytokine Adsorption Capacity of Octacalcium Phosphate by Interaction-Controlled Glycosaminoglycan Modification to Promote Osteoblastic Differentiation Peer-reviewed

    Ryo Hamai, Kaori Tsuchiya, Osamu Suzuki

    Langmuir　40　(52)　27253-27269　2024/12/18

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.langmuir.4c03067 　

    ISSN： 0743-7463

    eISSN： 1520-5827

  9. Efficacy of Octacalcium Phosphate/Gelatin (OCP/Gel) Composite Implantation for Miniature Swine Lumbar Interbody Fusion Peer-reviewed

    Yu Mori, Ryo Hamai, Ryuichi Kanabuchi, Takahiro Onoki, Kohei Takahashi, Ko Hashimoto, Toshimi Aizawa, Osamu Suzuki

    The Tohoku Journal of Experimental Medicine　2024

    Publisher: Tohoku University Medical Press

    DOI： 10.1620/tjem.2024.j138 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  10. Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity Peer-reviewed

    Takanori Hatakeyama, Ryo Hamai, Yukari Shiwaku, Takahisa Anada, Susumu Sakai, Tomoya Sato, Kazuyoshi Baba, Keiichi Sasaki, Osamu Suzuki

    Science and Technology of Advanced Materials　24　(1)　2277675　2023/11/27

    Publisher: Informa UK Limited

    DOI： 10.1080/14686996.2023.2277675 　

    ISSN： 1468-6996

    eISSN： 1878-5514

  11. Adsorption behavior and osteoblastic cellular activity of metronidazole molecule with octacalcium phosphate and hydroxyapatite materials Peer-reviewed

    Danupong Chaiariyakul, Ryo Hamai, Yukari Shiwaku, Kaori Tsuchiya, Osamu Suzuki

    Journal of Drug Delivery Science and Technology　89　105046-105046　2023/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.jddst.2023.105046 　

    ISSN： 1773-2247

  12. The material design of octacalcium phosphate bone substitute: increased dissolution and osteogenecity Peer-reviewed

    Osamu Suzuki, Ryo Hamai, Susumu Sakai

    Acta Biomaterialia　158　1-11　2023/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.actbio.2022.12.046 　

    ISSN： 1742-7061

  13. Effect of Octacalcium Phosphate Crystals on the Osteogenic Differentiation of Tendon Stem/Progenitor Cells In Vitro Peer-reviewed

    Xianchen Liu, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki

    International Journal of Molecular Sciences　24　(2)　1235-1235　2023/01/08

    Publisher: MDPI AG

    DOI： 10.3390/ijms24021235 　

    eISSN： 1422-0067

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    Synthetic octacalcium phosphate (OCP) activates bone tissue-related cells, such as osteoblasts, osteoclasts, and vascular endothelial cells. However, the effect of OCP on tendon-related cell activation remains unknown. This study examined the response of rat tendon stem/progenitor cells (TSPCs) to OCP and related calcium phosphate crystals in vitro. TSPCs were cultured with OCP and Ca-deficient hydroxyapatite (CDHA) obtained from the original OCP hydrolysis to assess the activity of alkaline phosphatase (ALP) and the expression of osteogenesis-related genes. Compared with CDHA, the effect of OCP on promoting the osteogenic differentiation of TSPCs was apparent: the ALP activity and mRNA expression of RUNX2, Col1a1, OCN, and OPN were higher in OCP than in CDHA. To estimate the changes in the chemical environment caused by OCP and CDHA, we measured the calcium ion (Ca2+) and inorganic phosphate (Pi) ion concentrations and pH values of the TSPCs medium. The results suggest that the difference in the osteogenic differentiation of the TSPCs is related to the ionic environment induced by OCP and CDHA, which could be related to the progress of OCP hydrolysis into CDHA. These results support the previous in vivo observation that OCP has the healing function of rabbit rotator cuff tendon in vivo.

  14. Impact of Octacalcium Phosphate/Gelatin (OCP/Gel) Composite on Bone Repair in Refractory Bone Defects Peer-reviewed

    Yu Mori, Ryo Hamai, Toshimi Aizawa, Osamu Suzuki

    The Tohoku Journal of Experimental Medicine　2023

    Publisher: Tohoku University Medical Press

    DOI： 10.1620/tjem.2023.j040 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  15. Osteogenic capacity of octacalcium phosphate involving macrophage polarization Peer-reviewed

    Linghao Xiao, Yukari Shiwaku, Ryo Hamai, Kazuyoshi Baba, Kaori Tsuchiya, Satoshi Imazato, Keiichi Sasaki, Osamu Suzuki

    Journal of Biomedical Materials Research Part A　111　(7)　1006-1020　2022/12/27

    Publisher: Wiley

    DOI： 10.1002/jbm.a.37484 　

    ISSN： 1549-3296

    eISSN： 1552-4965

  16. Octacalcium Phosphate/Gelatin Composite (OCP/Gel) Enhances Bone Repair in a Critical-Sized Transcortical Femoral Defect Rat Model Peer-reviewed

    Soshi Hamada, Yu Mori, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Kazuyoshi Baba, Itsuki Oizumi, Ryuichi Kanabuchi, Naohisa Miyatake, Toshimi Aizawa, Osamu Suzuki

    Clinical Orthopaedics and Related Research　480　(10)　2043-2055　2022/05/30

    Publisher: Ovid Technologies (Wolters Kluwer Health)

    DOI： 10.1097/corr.0000000000002257 　

    ISSN： 0009-921X

    eISSN： 1528-1132

  17. Differentiation of committed osteoblast progenitors by octacalcium phosphate compared to calcium-deficient hydroxyapatite in Lepr-cre/Tomato mouse tibia International-journal Peer-reviewed

    Kyosuke Okuyama, Yukari Shiwaku, Ryo Hamai, Toshihide Mizoguchi, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki

    Acta Biomaterialia　142　332-344　2022/04

    Publisher: Elsevier BV

    DOI： 10.1016/j.actbio.2022.02.016 　

    ISSN： 1742-7061

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    This study aimed to investigate the accumulation and differentiation of mesenchymal stem cells (MSCs) around octacalcium phosphate (OCP) compared with those around calcium-deficient hydroxyapatite (CDHA), a material obtained through hydrolysis of the original OCP. Leptin receptor (Lepr)-expressing bone marrow-derived MSCs around the OCP and CDHA were pursued utilizing genetically modified Lepr-cre/Tomato mice. OCP and CDHA granules were implanted into the tibia defect of the mice for 10 weeks and subjected to histomorphometric and immunohistochemical analyses. The structural properties of OCP and CDHA after inoculation into mouse subcutaneous tissue (until 4 weeks) or culture mediums (14 days) were analyzed using physicochemical techniques. In vitro osteoblastic differentiation of primary MSCs was examined with the materials for 14 days. While Lepr-cre/Tomato positive cells (red) accumulated around both OCP and CDHA, Lepr and osteocalcin double-positive osteoblastic cells (yellow) were significantly more abundant around OCP than around CDHA in the early implantation period. OCP enhanced the osteoblastic differentiation of MSCs more than CDHA in vitro. Physicochemical and structual analyses provided evidence that OCP tended to convert to the apatitic phase in the tested physiological environments. The higher osteoconductivity of OCP originated from a capacity-enhancing osteoblastic differentiation of committed osteoblast progenitors in bone marrow accompanied by OCP hydrolysis. STATEMENT OF SIGNIFICANCE: MSCs play a key role in bone regeneration through osteoblastic differentiation. Calcium phosphates have been widely applied as bone substitute materials, and OCP has a better ability to promote osteoblast differentiation of MSCs than that of HA in vitro. However, it is not clear how MSCs accumulate in the bone marrow and differentiate into osteoblasts during bone regeneration in vivo. In this study, we focused on the leptin receptor, a marker of bone marrow-derived MSCs. Using genetically modified mice labeled with the red fluorescent protein Tomato, we observed the accumulation of MSCs around calcium phosphates implanted in tibia bone defects and their differentiation into osteoblasts.

  18. Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity Peer-reviewed

    Ryo Hamai, Susumu Sakai, Yukari Shiwaku, Takahisa Anada, Kaori Tsuchiya, Takuya Ishimoto, Takayoshi Nakano, Osamu Suzuki

    Applied Materials Today　26　101279-101279　2022/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.apmt.2021.101279 　

    ISSN： 2352-9407

  19. Angio-osteogenic capacity of octacalcium phosphate co-precipitated with copper gluconate in rat calvaria critical-sized defect International-journal Peer-reviewed

    Shinki Koyama, Ryo Hamai, Yukari Shiwaku, Tsuyoshi Kurobane, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki

    Science and Technology of Advanced Materials　23　(1)　120-139　2022/02/01

    Publisher: Informa UK Limited

    DOI： 10.1080/14686996.2022.2035193 　

    ISSN： 1468-6996

    eISSN： 1878-5514

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    The objective of this study is to investigate the effects of octacalcium phosphate (OCP)-induced bone regeneration on angiogenesis regulated by the inclusion of copper ions in OCP in vitro and in vivo. Calcium (Ca)-deficient Cu-OCPs, containing 0.01 wt% Cu (low-Cu-OCP) and 0.12 wt% Cu (high-Cu-OCP), were synthesized with co7pper gluconate salt. The lattice parameters of Cu-OCPs tended to decrease slightly with Cu inclusion, as estimated by Rietveld analysis. Cu ions were released in OCP when the materials were incubated in the medium for human umbilical vein endothelial cells (HUVECs). The solubility of Cu-OCPs, estimated by the degree of supersaturation, was slightly higher than that of the original OCP. Cu-OCP tended to hydrolyze to an apatite structure while maintaining the crystal plate-like morphology when incubated with mesenchymal stem D1 cells in osteogenic media for 14 days. The specimens were characterized by selected area electron diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. Low-Cu-OCP significantly enhanced the HUVEC capillary cross-linking density. D1 cell differentiation was inhibited with the inclusion of Cu, even at low concentrations. The composite of low-Cu-OCP with a gelatin sponge (low-Cu-OCP/Gel) significantly enhanced angiogenesis coupled with bone regeneration when implanted in a rat calvarial critical-sized defect for 4 weeks, compared with the corresponding amount of Cu-containing Gel (Cu/Gel) or OCP/Gel materials through angiography and tissue histomorphometry. These results support the proposition that angiogenesis stimulated by low-Cu-OCP is closely related with enhanced bone regeneration.

  20. Macrophage Polarization Related to Crystal Phases of Calcium Phosphate Biomaterials Peer-reviewed

    Linghao Xiao, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Keiichi Sasaki, Osamu Suzuki

    International Journal of Molecular Sciences　22　(20)　11252-11252　2021/10/19

    Publisher: MDPI AG

    DOI： 10.3390/ijms222011252 　

    eISSN： 1422-0067

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    Calcium phosphate (CaP) materials influence macrophage polarization during bone healing. However, the effect of the crystal phase of CaP materials on the immune response of bone remains unclear. In this study, the effect of the crystal phases of CaP materials on the regulation of macrophage polarization was investigated. Human THP-1 cells and mouse RAW 264 cells were cultured with octacalcium phosphate (OCP) and its hydrolyzed form Ca-deficient hydroxyapatite to assess the expression of pro-inflammatory M1 and anti-inflammatory M2 macrophage-related genes. OCP inhibited the excessive inflammatory response and switched macrophages to the anti-inflammatory M2 phenotype, which promoted the expression of the interleukin 10 (IL10) gene. In contrast, HL stimulated an excessive inflammatory response by promoting the expression of pro-inflammatory M1 macrophage-related genes. To observe changes in the microenvironment induced by OCP and HL, inorganic phosphate (Pi) and calcium ion (Ca2+) concentrations and pH value in the medium were measured. The expression of the pro-inflammatory M1 macrophage-related genes (tumor necrosis factor alpha (TNFα) and interlukin 1beta (IL1β)) was closely related to the increase in ion concentration caused by the increase in the CaP dose. Together, these results suggest that the microenvironment caused by the crystal phase of CaP materials may be involved in the immune-regulation capacity of CaP materials.

  21. Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area International-journal Peer-reviewed

    Yukari Shiwaku, Ryo Hamai, Shinichi Sato, Susumu Sakai, Kaori Tsuchiya, Kazuyoshi Baba, Tetsu Takahashi, Osamu Suzuki

    International Journal of Molecular Sciences　22　(18)　9770-9770　2021/09/09

    Publisher: MDPI AG

    DOI： 10.3390/ijms22189770 　

    eISSN： 1422-0067

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    The microstructure of biomaterials influences the cellular and biological responses in the bone. Octacalcium phosphate (OCP) exhibits higher biodegradability and osteoconductivity than hydroxyapatite (HA) during the conversion process from OCP to HA. However, the effect of the microstructure of OCP crystals on long tubular bones has not been clarified. In this study, two types of OCPs with different microstructures, fine-OCP (F-OCP) and coarse-OCP (C-OCP), were implanted in rat tibia for 4 weeks. F-OCP promoted cortical bone regeneration compared with C-OCP. The osteoclasts appearance was significantly higher in the C-OCP group than in the control group (defect only) at 1-week post-implantation. To investigate whether the solubility equilibrium depends on the different particle sizes of OCPs, Nano-OCP, which consisted of nanometer-sized OCPs, was prepared. The degree of supersaturation (DS) tended to decrease modestly in the order of C-OCP, F-OCP, and Nano-OCP with respect to HA and OCP in Tris-HCl buffer. F-OCP showed a higher phosphate ion concentration and lower calcium ion concentration after immersion in the buffer than C-OCP. The crystal structures of both OCPs tended to be converted to HA by rat abdominal implantation. These results suggest that differences in the microstructure of OCPs may affect osteoclastogenesis and result in osteoconductivity of this material in long tubular bone by altering dissolution behavior.

  22. Involvement of distant octacalcium phosphate scaffolds in enhancing early differentiation of osteocytes during bone regeneration International-journal Peer-reviewed

    Shizu Saito, Ryo Hamai, Yukari Shiwaku, Tomoka Hasegawa, Susumu Sakai, Kaori Tsuchiya, Yuko Sai, Ryosuke Iwama, Norio Amizuka, Tetsu Takahashi, Osamu Suzuki

    Acta Biomaterialia　129　309-322　2021/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.actbio.2021.05.017 　

    ISSN： 1742-7061

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    This study hypothesized that distant octacalcium phosphate (OCP) scaffolds may enhance osteocyte differentiation in newly formed bone matrices. The results obtained were compared with those of Ca-deficient hydroxyapatite (OCP hydrolyzate, referred to as HL hereafter). Granular OCP and HL, 300-500 µm in diameter, were implanted in critical-sized rat calvarial defects for eight weeks and subjected to histology, immunohistochemistry, histomorphometry, and transmission electron microscopy (TEM). Early osteocyte differentiation from an osteoblastic cell line (IDG-SW3) was examined using materials without contacting the surfaces for 10 days. The material properties and the medium composition were analyzed through selected area electron diffraction (SAED) using TEM observation and curve fitting of Fourier transform infrared (FT-IR) spectroscopy. The number of positive cells of an osteocyte earlier differentiation marker podoplanin (PDPN) in bone matrices, along the direction of bone formation, was significantly higher in OCP than that in HL. The ultrastructure around the OCP surfaces observed by TEM showed the infiltration of some cells, including osteocytes adjacent to the OCP surface layers. The OCP structure remained unchanged by SAED analysis. Nanoparticle deposition and hydrolysis on OCP surfaces were detected by TEM and FT-IR, respectively, during early osteocyte differentiation in vitro. The medium saturation degree varied in accord with ionic dissolution, resulting in possible hydroxyapatite formation on OCP but not on HL. These results suggested that OCP stimulates early osteocyte differentiation in the bone matrix from a distance through its metastable chemical properties. STATEMENT OF SIGNIFICANCE: This study demonstrated that octacalcium phosphate (OCP) implanted in critical-sized rat calvaria bone defects is capable of enhancing the early differentiation of osteocytes embedded in newly formed bone matrices, even when the surface OCP is separated from the osteocytes. This prominent bioactive property of OCP was demonstrated by comparing the in vivo and in vitro performances with a control material, Ca-deficient hydroxyapatite (OCP hydrolyzate). The findings were elucidated by histomorphometry, which analyzed the differentiation of osteocytes along the parallel direction of new bone growth by osteoblasts. Therefore, OCP should stimulate osteocyte differentiation through ionic dissolution even in vivo owing to its metastable chemical properties, as previously reported in an in vitro study (Acta Biomater 69:362, 2018).

  23. Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration Peer-reviewed

    Hisashi Ozaki, Ryo Hamai, Yukari Shiwaku, Susumu Sakai, Kaori Tsuchiya, Osamu Suzuki

    Science and Technology of Advanced Materials　22　(1)　345-362　2021/04/16

    Publisher: Informa UK Limited

    DOI： 10.1080/14686996.2021.1916378 　

    ISSN： 1468-6996

    eISSN： 1878-5514

  24. Impact of simultaneous hydrolysis of OCP and PLGA on bone induction of a PLGA-OCP composite scaffold in a rat femoral defect Peer-reviewed

    Itsuki Oizumi, Ryo Hamai, Yukari Shiwaku, Yu Mori, Takahisa Anada, Kazuyoshi Baba, Naohisa Miyatake, Soshi Hamada, Kaori Tsuchiya, Shin-nosuke Nishimura, Eiji Itoi, Osamu Suzuki

    Acta Biomaterialia　124　358-373　2021/02

    Publisher: Elsevier BV

    DOI： 10.1016/j.actbio.2021.01.048 　

    ISSN： 1742-7061

    eISSN： 1878-7568

  25. Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials. Peer-reviewed

    Osamu Suzuki, Yukari Shiwaku, Ryo Hamai

    Dental materials journal　39　(2)　187-199　2020/03/31

    DOI： 10.4012/dmj.2020-001 　

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    Octacalcium phosphate (OCP) is a material that can be converted to hydroxyapatite (HA) under physiological environments and is considered a mineral precursor to bone apatite crystals. The structure of OCP consists of apatite layers stacked alternately with hydrated layers, and closely resembles the structure of HA. The performance of OCP as a bone substitute differs from that of HA materials in terms of their osteoconductivity and biodegradability. OCP manifests a cellular phagocytic response through osteoclast-like cells similar to that exhibited by the biodegradable material β-tricalcium phosphate (β-TCP). The use of OCP for human cranial bone defects involves using its granule or composite form with one of the natural polymers, viz., the reconstituted collagen. This review article discusses the differences and similarities in these calcium phosphate (Ca-P)-based materials from the viewpoint of the structure and their material chemistry, and attempts to elucidate why Ca-P materials, particularly OCP, display unique osteoconductive property.

  26. Chemical Stability-Sensitive Osteoconductive Performance of Octacalcium Phosphate Bone Substitute in an Ovariectomized Rat Tibia Defect Peer-reviewed

    Kazuyoshi Baba, Yukari Shiwaku, Ryo Hamai, Yu Mori, Takahisa Anada, Kaori Tsuchiya, Itsuki Oizumi, Naohisa Miyatake, Eiji Itoi, Osamu Suzuki

    ACS Applied Bio Materials　3　(3)　1444-1458　2020

    DOI： 10.1021/acsabm.9b01091 　

    eISSN： 2576-6422

  27. Comparative analysis of bovine serum albumin adsorption onto octacalcium phosphate crystals prepared using different methods Peer-reviewed

    Kaori TSUCHIYA, Ryo HAMAI, Susumu SAKAI, Osamu SUZUKI

    Dental Materials Journal　39　(5)　883-891　2020

    Publisher: Japanese Society for Dental Materials and Devices

    DOI： 10.4012/dmj.2019-250 　

    ISSN： 0287-4547

    eISSN： 1881-1361

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    This study compared bovine serum albumin (BSA) adsorption onto octacalcium phosphate (OCP) materials prepared from two wet preparations in the absence (w-OCP) and presence (c-OCP) of gelatin. Raman spectroscopy was used to analyze the BSA adsorption onto OCPs in a 150 mM Tris-HCl buffer containing 0.5 mM calcium and inorganic phosphate (Pi) ions at pH 7.4 and at 37°C. The degree of supersaturation of the supernatants after the adsorption was determined by measuring the ion composition. The results showed that BSA adsorption onto w-OCP was higher than that for c-OCP. The calcium ion concentration of the supernatant decreased for both w-OCP and c-OCP, whereas the Pi ion concentration increased, approaching OCP equilibria at different saturation levels. BSA adsorbed even onto c-OCP, which included a small amount of gelatin during c-OCP preparation. These results indicate that the biodegradability of w-OCP and c-OCP may be modulated through interactions with serum proteins.

  28. Adsorption of serum albumin onto octacalcium phosphate in supersaturated solutions regarding calcium phosphate phases Peer-reviewed

    Ryo Hamai, Kaori Tsuchiya, Osamu Suzuki

    Materials　12　(14)　2333　2019/07/01

    DOI： 10.3390/ma12142333 　

    eISSN： 1996-1944

  29. Angiogenesis involvement by octacalcium phosphate-gelatin composite-driven bone regeneration in rat calvaria critical-sized defect. International-journal Peer-reviewed

    Tsuyoshi Kurobane, Yukari Shiwaku, Takahisa Anada, Ryo Hamai, Kaori Tsuchiya, Kazuyoshi Baba, Masahiro Iikubo, Tetsu Takahashi, Osamu Suzuki

    Acta biomaterialia　88　514-526　2019/04/01

    DOI： 10.1016/j.actbio.2019.02.021 　

    ISSN： 1742-7061

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    Effect of octacalcium phosphate/gelatin composite (OCP/Gel) on angiogenesis was studied by its implantation in rat calvaria critical-sized defect in relation to bone regeneration for 2 and 4 weeks. The implantation of OCP/Gel disks was analyzed by radiomorphometry using a radiopaque material perfusion (Microfil®) method and histomorphometry by hematoxylin and eosin-staining before and after the decalcification. Effect of the OCP dose in the range up to 4 mg per well on the capillary-like tube formation by human umbilical vein endothelial cells (HUVECs) was also examined in a transwell cell culture. The results showed that the blood vessels formation by OCP/Gel group was significantly higher at 2 weeks than other groups but decreased at 4 weeks during the advancement of new bone formation. The capillary-like tube formation was highest in an OCP dose of 1 mg per well while other OCP doses above or below 1 mg did not show such a stimulatory effect. The results established both in vivo and in vitro confirmed that OCP has a positive effect on angiogenesis during bone regeneration in a suitable dose ranges, suggesting that the angiogenesis stimulated by OCP could be involved in the OCP/Gel-enhanced bone regeneration. STATEMENT OF SIGNIFICANCE: We have reported that octacalcium phosphate (OCP) materials display stimulatory capacities on the bone tissue-related cells. However, the effect of OCP on the angiogenesis and its relation to the OCP-enhanced bone regeneration is unknown. This study confirmed the capacity of OCP on angiogenesis before increasing the new bone mass after the implantation of a composite of OCP and gelatin (OCP/Gel). The blood vessels formation took place associated with the area beginning of the new bone formation, which finally decreased together with development of bone formation. Because OCP was ascertained stimulating the capillary-like tube formation in HUVEC culture with a certain OCP dose, the present study is the first report showing the capacity of OCP on angiogenesis during the OCP/Gel-enhanced bone regeneration.

  30. Culture of hybrid spheroids composed of calcium phosphate materials and mesenchymal stem cells on an oxygen-permeable culture device to predict in vivo bone forming capability. International-journal Peer-reviewed

    Tomoya Sato, Takahisa Anada, Ryo Hamai, Yukari Shiwaku, Kaori Tsuchiya, Susumu Sakai, Kazuyoshi Baba, Keiichi Sasaki, Osamu Suzuki

    Acta biomaterialia　88　477-490　2019/04/01

    DOI： 10.1016/j.actbio.2019.03.001 　

    ISSN： 1742-7061

    More details Close

    Three-dimensional (3-D) cell culture can better mimic physiological conditions in which cells interact with adjacent cells and the extracellular matrix than monolayer culture. We have developed a 3-D cell culture device, the Oxy chip, which can be used to generate and supply oxygen to cell spheroids to prevent hypoxia. Here, we used the Oxy chip to generate hybrid spheroids comprising calcium phosphate (CaP) particles (hydroxyapatite (HA), β-tricalcium phosphate (β-TCP) or octacalcium phosphate (OCP)) and mesenchymal stem cells (MSCs, C3H10T1/2 cells or D1 cells) that can be used to analyze cell differentiation mechanisms. We showed that the 3-D cell-cell and cell-material interactions and oxygenation offered by the Oxy chip promoted osteoblastic differentiation of MSCs. We also used histomorphometric analysis of hematoxylin and eosin staining, quality analyses by μCT and collagen orientation observation with picrosirius red staining in bone regeneration following implantation of three CaPs in a critical-sized defect in mouse calvaria. The in vivo bone formation capacity of the three tested CaP materials was OCP ≥ β-TCP > HA: the newly formed bone by OCP had a structure relatively close to that of the calvaria intact bone. When MSCs were 3-D cultured with the CaP materials using the Oxy chip, the in vitro osteogenic capacity of these materials was highly similar to trends observed in vivo. The in vitro alkaline phosphatase activity of D1 cells had the highest correlation with in vivo bone volume (R = 0.900). Chemical and FTIR spectroscopic analyses confirmed that differentiation of D1 cells could be associated with amorphous calcium phosphate (ACP) precipitation concomitant with OCP hydrolysis. Taken together, hybrid spheroid cultures using the Oxy chip can be used to screen and predict bone forming potential of bone substitute materials. STATEMENT OF SIGNIFICANCE: An oxygen permeable-culture chip (Oxy chip) can be used to induce formation of cell spheroids by mesenchymal stem cells (MSCs). Use of the Oxy chip avoids hypoxia in the spheroid core and enhances MSC osteoblastic differentiation relative to conventional spheroid culture methods. The present study showed that the Oxy chip mimics the in vivo environment associated with bone formation and can be used to generate hybrid spheroids consisting of calcium phosphates and MSCs that are useful for analyzing cell differentiation mechanisms. Bone formation analysis following implantation of calcium phosphate materials in mouse calvaria defects showed positive correlation with the in vitro results. We propose that hybrid spheroids cultured on the Oxy chip can be used to screen and predict the bone forming potential of bone substitute materials.

  31. Structural Effects of Sulfur-Containing Functional Groups on Apatite Formation on Ca2+-Modified Copolymers in a Simulated Body Environment Peer-reviewed

    Ryo Hamai, Yuki Shirosaki, Toshiki Miyazaki

    ACS Omega　3　(5)　5627-5633　2018/05/31

    DOI： 10.1021/acsomega.8b00694 　

    ISSN： 2470-1343

    eISSN： 2470-1343

  32. Structural effects of phosphate groups on apatite formation in a copolymer modified with Ca2+ in a simulated body fluid Peer-reviewed

    Ryo Hamai, Hirotaka Maeda, Hikaru Sawai, Yuki Shirosaki, Toshihiro Kasuga, Toshiki Miyazaki

    Journal of Materials Chemistry B　6　(1)　174-182　2018

    DOI： 10.1039/c7tb02363d 　

    ISSN： 2050-7518

    eISSN： 2050-750X

  33. Apatite formation on a hydrogel containing sulfinic acid group under physiological conditions Peer-reviewed

    Ryo Hamai, Yuki Shirosaki, Toshiki Miyazaki

    Journal of Biomedical Materials Research - Part B Applied Biomaterials　105　(7)　1924-1929　2017/10

    DOI： 10.1002/jbm.b.33732 　

    ISSN： 1552-4973

    eISSN： 1552-4981

  34. Apatite-forming ability of vinylphosphonic acid-based copolymer in simulated body fluid: effects of phosphate group content Peer-reviewed

    Ryo Hamai, Yuki Shirosaki, Toshiki Miyazaki

    Journal of Materials Science: Materials in Medicine　27　(10)　152　2016/10/01

    DOI： 10.1007/s10856-016-5761-y 　

    ISSN： 0957-4530

    eISSN： 1573-4838

  35. Biomineralization behavior of a vinylphosphonic acid-based copolymer added with polymerization accelerator in simulated body fluid Peer-reviewed

    Ryo Hamai, Yuki Shirosaki, Toshiki Miyazaki

    Journal of Asian Ceramic Societies　3　(4)　407-411　2015/12

    DOI： 10.1016/j.jascer.2015.09.001 　

    ISSN： 2187-0764

    eISSN： 2187-0764

  36. Morphology changing at incipient crystallization condition Peer-reviewed

    Takeshi Toshima, Ryo Hamai, Saya Fujita, Yuka Takemura, Saori Takamatsu, Masamoto Tafu

    Journal of Physics: Conference Series　596　(1)　2015

    DOI： 10.1088/1742-6596/596/1/012009 　

    ISSN： 1742-6588

    eISSN： 1742-6596

  37. Morphology control of brushite prepared by aqueous solution synthesis Peer-reviewed

    T. Toshima, R. Hamai, M. Tafu, Y. Takemura, S. Fujita, T. Chohji, S. Tanda, S. Li, G. W. Qin

    Journal of Asian Ceramic Societies　2　(1)　52-56　2014/03

    DOI： 10.1016/j.jascer.2014.01.004 　

    ISSN： 2187-0764

  38. Flash synthesis of non-trivial shaped oxide materials Peer-reviewed

    T. Toshima, T. Nakamura, R. Hamai, M. Tafu, T. Choji, Y. Nakammura, L. Song, Q.W. Gaowu

    Journal of Ecotechnology Research　17　(2)　89-93　2014

    Publisher: International Association of Ecotechnology Research

    DOI： 10.11190/jer.17.89 　

    ISSN： 1881-9982

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    In this study, we report spherical shaped oxide materials obtained by flash synthesis. The diameter of spherical particles is in the range 100nm to 100 μm and their surface structure is nano-fiber network, aggregation of nano-cubes and smooth types. This method is simple combustion synthesis in which a mixture of starting materials is combusted by external heating and ignited one end, combustion wave propagates from the end to the other. The starting mixtures are evaporated and form droplet (spherical shape), and then quenched on the surface of collection trap. Because of the quenching, global structure (spherical shape) is kept and the shape of droplet acts as template. We have succeeded the synthesis of spherical shaped SrTiO3, TiO2, CaTiO3 and Fe2O3 particles. Especially, in the case of synthesis with Ti powder in starting mixture, we also succeeded the synthesis of spherical hollow shaped particles.

  39. Effect of anions on morphology control of brushite particles Peer-reviewed

    Ryo Hamai, Takeshi Toshima, Masamoto Tafu, Takaaki Masutani, Tetsuji Chohji

    Key Engineering Materials　529-530　(1)　55-60　2013

    DOI： 10.4028/www.scientific.net/KEM.529-530.55 　

    ISSN： 1013-9826

    eISSN： 1662-9795

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Misc. 26

  1. 共存する材料・生体分子の物理化学的挙動の調節を介したリン酸八カルシウムによる骨再生 Invited

    濱井 瞭, 鈴木 治

    バイオマテリアル-生体材料-　41　132-135　2023/04

  2. リン酸八カルシウムと骨組織の相互作用がもたらす骨形成化学環境

    濱井 瞭, 尾崎 尚, 鈴木 治

    バイオマテリアル-生体材料-　41　26-27　2023/01

  3. リン酸八カルシウム（OCP）による骨再生のメカニズム解明と新機能開拓への取り組み

    鈴木 治, 濱井 瞭, 塩飽由香利

    バイオマテリアル-生体材料-　40　198-205　2022/07

  4. Regulation of Dissolution Behaviors and Bone Regeneration Capacities of Octacalcium Phosphate-based Composites Invited

    Ryo Hamai

    57　(7)　481-482　2022/07

  5. トピックス 転位導入によるリン酸八カルシウム 骨補填材の組織再生能強化

    鈴木 治, 濱井 瞭

    セラミックス　57　(4)　287　2022/04

  6. 生体内リン酸八カルシウムによる骨分化誘導効果

    奥山喬介, 奥山喬介, 塩飽由香利, 塩飽由香利, 濱井瞭, 溝口利英, 土屋香織, 高橋哲, 鈴木治

    日本再生医療学会総会(Web)　21st　2022

  7. リン酸八カルシウム周囲の間葉系幹細胞の局在と分化

    奥山喬介, 塩飽由香利, 濱井瞭, 溝口利英, 土屋香織, 高橋哲, 鈴木治

    日本口腔科学会学術集会プログラム・抄録集　76th　2022

  8. Effects of octacalcium phosphate on mouse bone marrow-derived mesenchymal stem cell differentiation and bone regeneration.

    奥山喬介, 奥山喬介, 奥山喬介, 塩飽由香利, 濱井瞭, 溝口利英, 土屋香織, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　44th　2022

  9. Role of mesenchymal stem cells in bone regeneration by octacalcium phosphate

    奥山喬介, 塩飽由香利, 塩飽由香利, 濱井瞭, 溝口利英, 高橋哲, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　43rd　2021

  10. Bone regeneration and osteocyte differentiation induced by octacalcium phosphate (OCP)

    塩飽由香利, 塩飽由香利, 齊藤志都, 濱井瞭, 長谷川智香, 網塚憲生, 蔡優広, 高橋哲, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　43rd　2021

  11. リン酸八カルシウム(OCP)が新生骨基質の骨細胞分化に及ぼす影響の検討

    齊藤志都, 齊藤志都, 塩飽由香利, 濱井瞭, 長谷川智香, 網塚憲生, 高橋哲, 鈴木治

    日本再生医療学会総会(Web)　20th　2021

  12. Effect of Surrounding Chemical Environment on Adsorption and Accumulation of Serum Protein onto Octacalcium Phosphate Crystals Peer-reviewed

    Ryo HAMAI, Kaori TSUCHIYA, Osamu SUZUKI

    Journal of the Japan Society of Powder and Powder Metallurgy　67　(5)　258-263　2020/05/15

    Publisher: Japan Society of Powder and Powder Metallurgy

    DOI： 10.2497/jjspm.67.258 　

    ISSN： 0532-8799

    eISSN： 1880-9014

  13. Analysis of osteocyte differentiation during bone formation by OCP implantation

    齊藤志都, 塩飽由香利, 濱井瞭, 高橋哲, 鈴木治, 長谷川智香, 網塚憲生

    日本セラミックス協会年会講演予稿集(CD-ROM)　2020　2020

  14. リン酸オクタカルシウムによって誘起される骨再生における早期組織応答

    黒羽根壮, 穴田貴久, 塩飽由香利, 濱井瞭, 飯久保正弘, 高橋哲, 鈴木治

    セラミックス基礎科学討論会講演要旨集　57th　206　2019/01

  15. 調製方法の異なるOCP/Gel複合体のラット頭蓋冠での骨形成の評価

    酒井進, 穴田貴久, 塩飽由香利, 濱井瞭, 土屋香織, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　40th　130 (WEB ONLY)　2018

  16. ハイブリッドスフェロイド培養法を用いたリン酸カルシウム系材料の骨再生能の評価

    佐藤智哉, 穴田貴久, 塩飽由香利, 濱井瞭, 土屋香織, 佐々木啓一, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　40th　424 (WEB ONLY)-424　2018

    Publisher: 日本バイオマテリアル学会

  17. 骨粗鬆症モデルラットによるリン酸オクタカルシウムの骨組織応答性に関する研究

    馬場一慈, 塩飽由香利, 穴田貴久, 濱井瞭, 森優, 井樋栄二, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　40th　218 (WEB ONLY)-218　2018

    Publisher: 日本バイオマテリアル学会

  18. 分子構造の異なるリン酸基を含有する有機‐無機複合体のアパタイト形成能

    濱井瞭, 城崎由紀, 宮崎敏樹

    日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM)　1F20　2016/08

  19. リン酸基含有有機‐無機ハイブリッドの作製と擬似体液中でのアパタイト形成能評価:リン酸基含有量の影響

    濱井瞭, 城崎由紀, 宮崎敏樹

    日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM)　1O18　2015/09

  20. 液相合成の混合法制御によるDCPD粒子の形状変化

    藤田沙也, 豊嶋剛司, 袋布昌幹, 竹村由香, 高松さおり, 濱井瞭, 横井太史, 丹田聡, LI S, QIN G.W

    日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM)　27th　1PQ05　2014/08

  21. リン酸基含有有機‐無機ハイブリッドの作製と擬似体液環境下におけるアパタイト形成能評価:重合促進剤の影響

    濱井瞭, 城崎由紀, 宮崎敏樹

    日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM)　2O06　2014/08

  22. 環境技術を指向したリン酸カルシウム塩の粒子形状制御技術

    袋布昌幹, 濱井瞭, 豊嶋剛司, 丁子哲治

    日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM)　26th　2A02　2013/08

  23. リン酸カルシウム複合体と水溶液中微量フッ素化合物との反応性評価

    桝谷峰旦, 濱井瞭, 豊嶋剛司, 袋布昌幹, 丁子哲治

    日本セラミックス協会秋季シンポジウム講演予稿集　24th　191-793　2011/09/07

    Publisher: The Ceramic Society of Japan

    DOI： 10.14853/pcersj.2011F.0.793.0 　

  24. Flashing法による球および球殻形状のSrTiO3結晶合成

    豊嶋剛司, 中村友一, 濱井瞭, 袋布昌幹, 丁子哲治, 中村雄一

    応用物理学会学術講演会講演予稿集(CD-ROM)　72　NO.30A-P2-1　2011/08

  25. Flashing法によるSrTiO3結晶合成と光物性評価

    大家雄太, 中村友一, 濱井瞭, 吉川文恵, 豊嶋剛司, 中村雄一

    応用物理学会学術講演会講演予稿集(CD-ROM)　72　NO.30A-P2-2　2011/08

  26. Reactivity of calcium phosphate hybrid with small amount of fluoride ion in an aqueous solution

    MASUTANI TAKAAKI, HAMAI RYO, TOSHIMA TAKESHI, TAFU MASAMOTO, CHOHJI TETSUJI

    Preprints of Annual Meeting of The Ceramic Society of Japan<br>Preprints of Fall Meeting of The Ceramic Society of Japan　2011　(0)　793-793　2011

    Publisher: The Ceramic Society of Japan

    DOI： 10.14853/pcersj.2011F.0.793.0 　

    More details Close

    リン酸水素カルシウム二水和物(DCPD)は，水溶液中で粒子表面に前駆体相の微粒子を生成した後，微量フッ化物イオンと選択的反応してフッ素アパタイト(FAp)を生成する．この反応を用いることにより汚染土壌，排水等のフッ素化合物の不溶化・処理が可能であることが見いだされているが，その製造条件等により反応性に大きな差違がみられる。 本報では種々の方法で合成・製造したDCPD塩を用いてそのフッ化物イオンの反応性を評価した。また，DCPDに水酸アパタイト（HA）など他のリン酸塩を複合化させる効果について検討した。

Show all ︎Show first 5

Books and Other Publications 3

  1. Octacalcium Phosphate Biomaterials

    R Hamai, T Anada, O Suzuki

    Elsevier　2019/11

  2. 無機 / 有機材料の表面処理・改質による生体適合性付与

    鈴木 治, 濱井 瞭

    シーエムシー出版　2019/05

  3. 再生医療の開発戦略と最新研究事例集

    鈴木 治, 濱井 瞭

    情報技術協会　2019/02

Presentations 65

  1. ケモカイン担持リン酸八カルシウムの加水分解が間葉系幹細胞の遊走に及ぼす影響の検討

    濱井 暸, 金淵龍一, 森 優, 濵田壮志, 塩飽由香利, 蔡 優広, 土屋香織, 相澤俊峰, 鈴木 治

    日本セラミックス協会2025年年会　2025/03/06

  2. 薬剤放出能を付与したリン酸八カルシウム/生体吸収性合成高分子複合体の作製

    木村太, 濱井 瞭, 岡田正弘, 土屋香織, 山内健介, 鈴木 治

    日本セラミックス協会2025年年会　2025/03/05

  3. Protein adsorption and osteoblastic differentiation on octacalcium phosphate via different hydrolysis processes

    Ryo Hamai, Masahiro Okada, Kaori Tsuchiya, Osamu Suzuki

    22nd Asian BioCeramics Symposium (ABC2024)　2024/12/04

  4. 薬剤放出能を付与したリン酸八カルシウム/PLGA 複合体による骨芽細胞分化

    木村 太, 濱井 瞭, 岡田正弘, 土屋香織, 山内健介, 鈴木 治

    日本バイオマテリアル学会シンポジウム2024　2024/10/29

  5. 間質由来因子-1 担持リン酸八カルシウムの骨髄由来細胞の遊走・分化の 調節と骨再生

    金淵龍一, 濱井 瞭, 塩飽由香利, 森 優, 土屋香織, 蔡 優広, 濵田壮志, 栗山恭明, 相澤俊峰, 鈴木治

    日本バイオマテリアル学会シンポジウム2024　2024/10/29

  6. Gelatin多孔体の分解産物による血管新生と骨再生

    畠山高徳, 濱井 瞭, 塩飽由香利, 穴田貴久, 酒井 進, 佐々木啓一, 鈴木 治

    日本バイオマテリアル学会シンポジウム2024　2024/10/28

  7. 荷重部への応用を目指したOCP/PLGA 複合体の検討

    林智洋, 濱井瞭, 叶内彩, 細谷敬三, 岡田正弘, 鈴木治

    日本バイオマテリアル学会シンポジウム2024　2024/10/28

  8. リン酸八カルシウムの加水分解プロセスがタンパク質吸着と骨芽細胞分 化に及ぼす影響

    濱井 瞭, 土屋香織, 鈴木 治

    日本バイオマテリアル学会シンポジウム2024　2024/10/28

  9. ケモカインを担持したリン酸八カルシウム/PLGA 複合体への骨組織応答の評価

    栗山 恭明, 濱井 瞭, 森 優, 馬場 一慈, 土屋 香織, 金淵 龍一, 原田 健登, 相澤, 俊峰, 鈴木 治

    日本バイオマテリアル学会シンポジウム2024　2024/10/28

  10. ケモカイン担持によるリン酸八カルシウム/PLGA複合体の骨再生能強化の検討

    栗山恭明, 濱井瞭, 森優, 馬場一慈, 土屋香織, 金淵龍, 原田健登, 相澤俊峰, 鈴木治

    セラミックス協会第37回秋季シンポジウム　2024/09/12

  11. Gelatin 多孔体の分解が血管新生と骨再生に及ぼす影響の検討

    濱井 瞭, 畠山高徳, 塩飽由香利, 穴田貴久, 酒井 進, 佐々木啓一, 鈴木 治

    日本歯科理工学会 令和6年度第82回学術講演会　2024/04/20

  12. リン酸八カルシウムと血清由来糖タンパク質との相互作用による間葉系幹細胞の骨芽細胞分化促進

    濱井 瞭, 坪井裕紀, 土屋香織, 山内健介, 鈴木 治

    日本セラミックス協会2024年会　2024/03/15

  13. ラット大腿骨両側皮質骨貫通欠損の修復に及ぼす リン酸八カルシウム/PLGA骨補填材とケモカインの併用効果

    栗山恭明, 濱井瞭, 森優, 馬場一慈, 土屋香織, 金淵龍一, 原田健登, 相澤俊峰, 鈴木治

    第45回東北骨代謝・骨粗鬆症研究会　2024/02/03

  14. リン酸八カルシウム周囲に存在するfetuinと骨伝導性に関する研究

    坪井裕紀, 濱井 瞭, 奥山喬介, 土屋香織, 山内健介, 鈴木 治

    第45回東北骨代謝・骨粗鬆症研究会　2024/02/03

  15. リン酸八カルシウムと⽣体材料の溶解・分解の相互調節と⾻再⽣ Invited

    濱井 瞭, 鈴木 治

    2023年度 東北大学金属材料研究所共同研究ワークショップ・日本バイオマテリアル学会東北ブロック交流会　2023/12/22

  16. ラット長管骨欠損におけるリン酸八カルシウムと間質由来因子1の骨再生に関する相乗効果

    金淵龍一, 濱井暸, 塩飽由香利, 森優, 土屋香織, 蔡優広, 濵田壮志, 栗山恭明, 原田健登, 相澤俊峰, 鈴木治

    第25回生体関連セラミックス討論会　2023/12/01

  17. ケモカイン担持リン酸八カルシウムのラット長管骨欠損修復能の評価

    金淵龍一, 濱井 瞭, 塩飽由香利, 森 優, 土屋香織, 蔡 優広, 濵田壮志, 栗山恭明, 原田健登, 相澤俊峰, 鈴木治

    第45回日本バイオマテリアル学会大会　2023/11/06

  18. ラット大腿骨皮質骨欠損におけるOCP/Gel, Gel, β-TCPの骨修復能の検討

    森優, 金淵龍一, 濱井瞭, 土屋香織, 馬場一慈, 濱田壮志, 相澤俊峰, 鈴木治

    第45回日本バイオマテリアル学会大会　2023/11/06

  19. リン酸八カルシウム（OCP）の連続フロー合成法の検討

    丸子夕夏, 林智洋, 濱井瞭, 鈴木治, 細谷敬三

    第45回日本バイオマテリアル学会大会　2023/11/06

  20. リン酸八カルシウム/iPS細胞ハイブリッドスフェロイドの3D培養による骨分化評価

    菅井優生, 濱井 瞭, 塩飽由香利, 穴田貴久, 土屋香織, 伹野愛実, 山内健介, 高橋 哲, 江草 宏, 鈴木 治

    第45回日本バイオマテリアル学会大会　2023/11/06

  21. 硫酸化多糖類修飾リン酸八カルシウムのサイトカイン吸着と骨芽細胞の活性化

    濱井 瞭, 土屋香織, 鈴木 治

    第45回日本バイオマテリアル学会大会　2023/11/06

  22. Continuous-Flow Synthesis of Octacalcium Phosphate

    Tomohiro Hayashi, Yuka Maruko, Keizo Hosoya, Ryo Hamai, Osamu Suzuki

    Bioceramics33　2023/10/18

  23. Increased osteogenecity of octacalcium phosphate by dislocation introduction obtained through a co-precipitation with gelatin molecules

    Osamu Suzuki, Ryo Hamai, Susumu Sakai, Yukari Shiwaku, Kaori Tsuchiya

    Bioceramics 33　2023/10/17

  24. Bioactivity and bioresorbability of octacalcium phosphate involving hydrolysis into apatite crystals

    Osamu Suzuki, Ryo Hamai, Yuakri Shiwaku

    2023/10/17

  25. 多糖類修飾リン酸八カルシウム表面における成長因子の局在状態調節と骨芽細胞の活性評価

    濱井 瞭, 土屋香織, 鈴木 治

    日本セラミック協会 第36回秋季シンポジウム　2023/09/08

  26. Antibacterial Agent Adsorption onto Octacalcium Phosphate and its Osteoblastic Differentiation Capacity

    Danupong Chaiariyakul, Ryo Hamai, Yukari Shiwaku, Kaori Tsuchiya, Osamu Suzuki

    2023/09/07

  27. リン酸八カルシウム/ゼラチン複合体における有機マトリックスの分解が骨再生に及ぼす影響の検討

    畠山高徳, 飛田 翼, 濱井 瞭, 塩飽由香利, 穴田貴久, 佐々木啓一, 鈴木 治

    日本セラミックス協会2023年会　2023/03/08

  28. Response of tendon stem/progenitor cells in the microenvironment of OCP hydrolysis

    Xianchen Liu, Yukari Shiwaku, Ryo Hamai, Tetsu Takahashi, Osamu Suzuki

    2023/02/04

  29. OCP/Gelatin複合体のラット大腿骨両側皮質骨欠損モデルに対する骨修復能の放射線学的，組織学的評価

    金淵龍一, 森優, 濱井瞭, 土屋香織, 宮武尚央, 馬場一慈, 大泉樹, 濵田壮志, 相澤俊峰, 鈴木治

    第44回 東北骨代謝・骨粗鬆症研究会　2023/02/04

  30. Osteogenesis and angiogenesis induced by octacalcium phosphate releasing copper ion Invited

    Ryo Hamai, Shinki Koyama, Yukari Shiwaku, Tsuyoshi Kurobane, Kaori Tsuchiya, Osamu Suzuki

    JSPS-NRF Bilateral Program Mini-Symposium on Advanced Biomaterials for Tissue Engineering, and Tissue Synthesis and Manipulation　2023/01/17

  31. Response of tendon stem/progenitor cells by octacalcium phosphate in vitro

    Xianchen Liu, Yukari Shiwaku, Ryo Hamai, Tetsu Takahashi, Osamu Suzuki

    20th Asian BioCeramics Symposium 2022　2022/12/11

  32. Investigation of the relationship between angiogenesis and osteogenesis induced by octacalcium phosphate containing copper ion

    Ryo Hamai, Shinki Koyama, Yukari Shiwaku, Tsuyoshi Kurobane, Kaori Tsuchiya, Osamu Suzuki

    20th Asian BioCeramics Symposium　2022/12/12

  33. リン酸オクタカルシウム/ゼラチン多孔質複合体を用いたラット大腿骨難治性骨欠損に対する骨修復効果について

    金淵龍一, 森優, 濱井瞭, 土屋香織, 宮武尚央, 馬場一慈, 大泉樹, 濵田壮志, 相澤俊峰, 鈴木治

    第41回 整形外科バイオマテリアル研究会　2022/12/10

  34. Evaluation of Antibiotic Adsorption and Release in Octacalcium Phosphate (OCP) and its Related Materials

    Danupong CHAIARIYAKUL, Ryo HAMAI, Yukari SHIWAKU, Kaori TSUCHIYA, Osamu SUZUKI

    2022/11/21

  35. 調整方法の異なるリン酸八カルシウム(OCP)の溶解特性と骨置換性の比較検討

    酒井 進, 濱井 瞭, 土屋 香織, 鈴木 治

    第44回日本バイオマテリアル学会大会　2022/11/22

  36. マウス骨髄由来間葉系幹細胞の分化と骨再生に及ぼすリン酸八カルシウムの効果

    奥山喬介, 塩飽由香利, 濱井 瞭, 溝口利英, 土屋香織, 鈴木 治

    第44回日本バイオマテリアル学会大会　2022/11/22

  37. 銅イオン含有リン酸八カルシウムの溶解が 血管新生に及ぼす影響の検討

    濱井 瞭, 髙山 慎騎, 塩飽 由香利, 黒羽根 壮, 土屋 香織, 鈴木 治

    第44回日本バイオマテリアル学会大会　2022/11/21

  38. Comparative study of dissolution and biodegradable properties of octacalcium phosphate (OCP) obtained through different preparation

    Susumu SAKAI, Ryo HAMAI, Osamu SUZUKI

    INTERNATIONAL DENTAL MATERIALS CONGRESS (IDMC) 2022　2022/11/05

  39. Adsorption and Release Behavior of Antibacterial Agent in Octacalcium Phosphate (OCP) and its Related Materials

    Danupong CHAIARIYAKUL, Ryo HAMAI, Yukari SHIWAKU, Osamu SUZUKI

    INTERNATIONAL DENTAL MATERIALS CONGRESS (IDMC) 2022　2022/11/05

  40. Mutual formation of osteogenic chemical environment by octacalcium phosphate and bone tissue Invited

    Ryo Hamai, Hisashi Ozaki, Kaori Tsuchiya, Osamu Suzuki

    2022 KSBM Fall Meeting & Tutorial Symposium　2022/09/29

  41. Analysis of osteoblastic differentiation and bone substitution by octacalcium phosphate synthesized in the presence of gelatin molecules

    O. Suzuki, R. Hamai, S. Sakai, Y. Shiwaku, K. Tsuchiya, T. Anada, T. Ishimoto, T. Nakano

    2022/09/16

  42. Analysis of lattice defects in octacalcium phosphate synthesized in the presence of gelatin molecules and evaluation of its dissolution behavior under physiological conditions

    Ryo Hamai, Kaori Tsuchiya, Osamu Suzuki

    2022/09/16

  43. Effect of angiogenesis induced by lower amount of copper ion on octacalcium phosphate induced-bone regeneration

    髙山慎騎, 濱井 瞭, 塩飽由香利, 黒羽根壮, 高橋 哲, 鈴木 治

    2022/05/22

  44. リン酸八カルシウムと骨髄間葉系幹細胞の挙動

    奥山喬介, 塩飽由香利, 濱井 瞭, 高橋 哲, 鈴木 治

    第79 回日本歯科理工学会学術講演会　2022/05/22

  45. Effects of OCP/PLGA Composites on MSC Differentiation and The Materials Hydrolyses

    Ryo Hamai, Itsuki Oizumi, Yukari Shiwaku, Yu Mori, Takahisa Anada, Kazuyoshi Baba, Naohisa Miyatake, Shin-nosuke Nishimura, Toshimi Aizawa, Eiji Itoi, Osamu Suzuki

    2022 JSB/SFB Joint Symposium　2022/01/08

  46. リン酸八カルシウム(OCP)による骨再生と骨細胞分化

    塩飽 由香利, 齊藤 志都, 濱井 瞭, 長谷川 智香, 網塚 憲生, 蔡 優広, 高橋 哲, 鈴木 治

    2021/11/30

  47. リン酸八カルシウムによる骨再生における血管新生の役割について

    髙山 慎騎, 濱井 瞭, 塩飽 由香利, 黒羽 根 壮, 土屋 香織, 高橋 哲, 鈴木 治

    2021/11/29

  48. リン酸八カルシウムによる骨再生における未分化 間葉系幹細胞の役割に関する研究

    奥山 喬介, 塩飽 由香利, 濱井 瞭, 溝口 利英, 高橋 哲, 鈴木 治

    2021/11/29

  49. Investigation of the effects of coexistence of octacalcium phosphate with bone tissue on mutual chemical behavior in vitro

    2021/09/01

  50. OCP/PLGA 複合体によるラット大腿骨規格化欠損の組織形態計測による骨再生評価

    2021/04/10

  51. Observation of bone regeneration by implantation of octacalcium phosphate material mixed with autologous bone in rat calvarial bone defect model

    H. Ozaki, R. Hamai, Y. Shiwaku, O. Suzuki

    2021/04/10

  52. リン酸八カルシウムの加水分解反応がポリ乳酸–グリコール酸共重合体の分解挙動に及ぼす影響の検討

    濱井 瞭, 大泉 樹, 塩飽由香利, 森 優, 穴田貴久, 馬場一慈, 土屋香織, 西村慎之介, 井樋栄二, 鈴木 治

    日本セラミックス協会 2021年会　2021/03/24

  53. リン酸八カルシウム（OCP）が新生骨基質の骨細胞分化に及ぼす影響の検討

    齊藤志都, 塩飽由香利, 濱井 瞭, 長谷川智香, 網塚憲生, 髙橋 哲, 鈴木 治

    第20回日本再生医療学会総会

  54. リン酸八カルシウム/PLGA複合体によるラット大腿骨貫通型骨欠損の修復に関する検討

    大泉樹, 濱井瞭, 塩飽由香利, 森優, 馬場一慈, 穴田貴久, 井樋栄二, 鈴木治

    第20回日本再生医療学会総会

  55. Comparative study of capacity of protein adsorption onto octacalcium phosphate prepared by different synthesis conditions

    Ryo Hamai, Kaori Tsuchiya, Susumu Sakai, Osamu Suzuki

    The 15th International Workshop on Biomaterials in Interface Science / The 11th Symposium on Innovative Dental-Engineering Alliance (IDEA)　2020/12/14

  56. タンパク質吸着能の変化がリン酸八カルシウムの溶解性に及ぼす影響の検討

    濱井 瞭, 土屋香織, 酒井 進, 鈴木 治

    日本セラミックス協会 第33回秋季シンポジウム

  57. 天然高分子との共沈により調節されるリン酸八カルシウム結晶の溶解性とタンパク質吸着挙動

    土屋 香織, 濱井 瞭, 酒井 進, 鈴木 治

    日本セラミックス協会 2020年年会　2020/03/18

  58. Dual Effect of Serum Protein Adsorption on Additional Crystal Formation on Octacalcium Phosphate in Supersaturated Condition

    Ryo Hamai, Kaori Tsuchiya, Osamu Suzuki

    2019/12/09

  59. 過飽和溶液中で血清タンパク質が吸着した リン酸八カルシウム表面での新生結晶形成挙動

    濱井瞭, 土屋香織, 鈴木治

    第41回バイオマテリアル学会大会　2019/11/25

  60. 【招待講演】リン酸八カルシウム結晶周囲の化学環境が血清タンパク質吸着と集積に及ぼす影響の検討 Invited

    濱井 瞭, 土屋 香織, 鈴木 治

    粉体粉末冶金協会2019 年度秋季大会　2019/10/24

  61. 合成方法の異なるリン酸八カルシウムの表面化学状態の比較

    酒井 進, 濱井 瞭, 塩飽由香利, 鈴木 治

    第74 日本歯科理工学会 秋季学術講演会　2019/10/05

  62. Evaluation of Surface Phosphate Species of Octacalcium Phosphate in Physiological Solutions

    Ryo Hamai, Susumu Sakai, Yukari Shiwaku, Kaori Tsuchiya, Osamu Suzuki

    The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-4)　2019/10/03

  63. リン酸カルシウムに対する過飽和度がリン酸八カルシウムのタンパク質吸着挙動に及ぼす影響の評価

    濱井瞭, 鈴木治

    第73回 日本歯科理工学会 春期学術講演会　2019/04/21

  64. リン酸八カルシウムと 薬剤含有生体吸収性高分子の複合体に関する研究

    木村 太, 濱井 瞭, 岡田正弘, 土屋香織, 山内健介, 鈴木 治

    令和6（2024）年度北海道・東北地方会セミナー　2024/10/05

  65. OCP骨補填材の適用範囲拡大を目指した検討について

    林智洋, 濱井瞭, 叶内 彩, 細谷敬三, 岡田正弘, 鈴木治

    令和6（2024）年度北海道・東北地方会セミナー　2024/10/05

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Research Projects 9

  1. Bone substitute materials that promote the materials resorption and new bone formation

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2024/04/01 - 2027/03/31

  2. Development of bone graft substitutes with both osteogenic potential and high strength

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2023/04/01 - 2026/03/31

  3. リン酸カルシウムの骨再生能強化と生体用高分子の吸収性制御を両立した複合材料の開発

    濱井 瞭

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究

    Category: 若手研究

    Institution: 東北大学

    2023/04/01 - 2026/03/31

  4. 骨再生能と高弾性を有する骨補填材開発の試み

    鈴木 治, 濱井 瞭, 塩飽 由香利, 森 優

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 東北大学

    2021/04/01 - 2024/03/31

  5. Fabrication of a bioactive calcium phosphate implant material

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2021/07/09 - 2023/03/31

  6. Evaluation of relationship between growth factor adsorption onto calcium phosphates exhibiting different solubility and osteoblastic differentiation

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Category: Grant-in-Aid for Early-Career Scientists

    Institution: Tohoku University

    2020/04 - 2023/03

  7. A study on bioactive ceramic biomaterials

    Suzuki Osamu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2018/04/01 - 2021/03/31

    More details Close

    The present study was designed to investigate about how physicochemical environments affect the activity of calcium phosphate materials. Calcium phosphate materials, including octacalcium phosphate (OCP), cultured with mesenchymal stem cells in the spheroid form, enhanced its osteoblastic differentiation in vitro. The cellular activity was well correlated with mouse calvaria defect bone formation by the materials. OCP/gelatin composite was capable of enhancing angiogenesis followed by bone formation in rat calvaria defect. The dose of OCP in the composite affected bone formation ability in rat model under osteoporotic serum environment. The chemical environment surrounding the bone substitute materials may affect the activity of calcium phosphate materials in particular OCP.

  8. 骨形成因子結合多糖の空間分布が異なるリン酸八カルシウム表面での骨形成挙動解明

    濱井 瞭

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 研究活動スタート支援

    Category: 研究活動スタート支援

    Institution: 東北大学

    2018/08/24 - 2020/03/31

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    本年度は，グリコサミノグリカン(GAG)とリン酸カルシウムとの結合形成の起点となるアミノ基の表面修飾方法について検討した。アミノ基を有する分子として，11-aminoundecylphosphonic acid (11-AUPA)または3-aminopropyltrimethoxysilane (3-APTS)を選択し，基材となるリン八酸カルシウム(OCP)表面へのアミノ基修飾を試みた。具体的には，11-AUPAもしくは3-APTSを溶解させた有機溶媒中でOCPを処理することで，表面修飾処理を施した。X線光電子分光やラマン分光分析により，OCP表面にそれぞれのアミノ基含有分子が修飾されたことが認められた。加えて，表面に修飾したアミノ基とGAGとの静電的相互作用による結合もしくは共有結合の形成に先立ち，修飾したアミノ基のリン酸緩衝生理食塩水(PBS)中における安定性を評価した。その結果，OCPの溶解もしくは修飾分子-基材間の結合の弱さに起因し，修飾した分子の安定性が乏しいことが示唆された。 このことから，修飾したアミノ基の安定性を向上させるため，化学修飾処理後のOCPの加熱処理もしくは，OCPよりも溶解度の低い基材への修飾を試みた。その結果，加熱処理によって，OCPに修飾した11-AUPAおよび3-APTSの安定性が改善することが示唆された。また，基材の溶解度によっても安定性が向上する傾向にあった。加えて，PBS中でのゼータ電位測定により，アミノ基の修飾量は，選択した修飾試薬の種類によって異なることも示唆された。 これら本年度に実施した検討により，基材固定型もしくは徐放型骨形成タンパク質結合GAGモデルの設計指針を見出すことができた。

  9. Quantitative elucidation of mechanism for osteoconductive induced by phosphorus-containing functional groups using polymer surface model

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows

    Category: Grant-in-Aid for JSPS Fellows

    Institution: Kyushu Institute of Technology

    2017/04/26 - 2019/03/31

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