TOHOKU UNIVERSITY Researchers - Yasuhiro Ishimaru

Details of the Researcher

Home

日本語 English

Yasuhiro Ishimaru

Section

Graduate School of Engineering

Job title

Associate Professor

Degree

博士（農学）（東京大学）
修士（農学）（東京大学）

researchmap

https://researchmap.jp/read0143827

J-GLOBAL ID

200901039157335920

e-Rad No.

80590207

Research History 5

2019/05 - Present

東北大学
2011/04 - 2021/04

The University of Tokyo　Graduate School of Agricultural and Life Sciences, Global Agricultural Sciences
2011/04 - 2019/04

Tohoku University　Graduate School of Science, Department of Chemistry
2006/08 - 2011/03

The University of Tokyo　Graduate School of Agricultural and Life Sciences, Global Agricultural Sciences
2006/04 - 2006/07

The University of Tokyo　Graduate School of Agricultural and Life Sciences, Global Agricultural Sciences

Education 4

The University of Tokyo　Graduate School of Agricultural and Life Sciences　Global Agricultural Sciences

2003/04 - 2006/03
The University of Tokyo　Graduate School of Agricultural and Life Sciences　Global Agricultural Sciences

2001/04 - 2003/03
The University of Tokyo

1999/04 - 2001/03
The University of Tokyo　College of Arts and Sciences

1997/04 - 1999/03

Committee Memberships 7

日本植物生理学会　第64回日本植物生理学会年会実行委員

2021/04 - 2023/03
日本農芸化学会　東北支部幹事

2019/05 - 2021/05
日本農芸化学会　大会実行委員会実行委員

2019/08 - 2021/03
日本生物工学会　第72回日本生物工学会大会実行委員

2019/04 - 2020/09
日本農芸化学会　東北支部選挙管理委員会委員

2019/08 - 2020/05
日本農芸化学会　東北支部大会実行委員会実行委員

2018/04 - 2019/03
日本土壌肥料学会　選挙管理委員会委員

2010/04 - 2011/03

Show all ︎Show first 5

Professional Memberships 3

THE JAPANESE SOCIETY FOR CHEMICAL REGULATION OF PLANTS
JAPANESE SOCIETY OF SOIL SCIENCE AND PLANT NUTRITION
JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

Research Interests 3

ion channel
transporter
plant

Research Areas 1

Life sciences / Plant nutrition, soil science /

Awards 6

農芸化学奨励賞

2018/12　日本農芸化学会
日本農芸化学会東北支部奨励賞

2018/08　日本農芸化学会
B.B.B.論文賞

2018/03　日本農芸化学会
Japan Prize in Agricultural Sciences, Achievement Award for Young Scientists

2011/09　JABEE
Encouraging prize

2009/09　Japanese Society of Soil Science and Plant Nutrition
Poster awards

2008/09　FASEB Trace Micronutrient

Show all ︎Show 5

Papers 71

Structure reveals a regulation mechanism of plant outward-rectifying K+ channel GORK by structural rearrangements in the CNBD-Ankyrin bridge. International-journal

Taro Yamanashi, Yuki Muraoka, Tadaomi Furuta, Tsukasa Kume, Natsuko Sekido, Shunya Saito, Shota Terashima, Takeshi Yokoyama, Yoshikazu Tanaka, Atsushi Miyamoto, Kanane Sato, Tomoyuki Ito, Hikaru Nakazawa, Mitsuo Umetsu, Ellen Tanudjaja, Masaru Tsujii, Ingo Dreyer, Julian I Schroeder, Yasuhiro Ishimaru, Nobuyuki Uozumi

Proceedings of the National Academy of Sciences of the United States of America　122　(30)　e2500070122　2025/07/29

DOI： 10.1073/pnas.2500070122 　

More details Close

Guard cells, which regulate stomatal apertures in plants, possess a sophisticated mechanism for regulating turgor pressure. The outward-rectifying "K+out" channel GORK, expressed in guard cells of the plant Arabidopsis thaliana, is a central component that promotes stomatal closure by releasing K+ to the extracellular space, thereby lowering turgor pressure. To date, the structural basis underlying the regulation of the K+ transport activity of GORK is unclear. Using cryo-EM, we determined the structures of the GORK outward-rectifying K+ channel with a resolution of 3.16 to 3.27 Å in five distinct conformations that differ significantly in their C-terminal cyclic nucleotide binding domain (CNBD) and ankyrin repeat (ANK) domain. The C-linker connects the transmembrane domains to the C-terminal domains, i.e., CNBD, CNBD-Ankyrin bridge, and ANK. The structural changes and interactions in the C-linker determine whether the closed state of GORK is closer to the preopen state or in a more removed state from the open state of the channel. In particular, interconversion in the short sequence within the CNBD-Ankyrin bridge plays a decisive role in this determination. This region forms an α-helix in the preopened state, while it adopts a nonhelical structure in further distant closed states. The dynamics of the cytosolic region strongly suggest that the K+ channel activity of GORK is regulated by cytosolic signaling factors during stomatal closure.
Functional characterization of an additional transmembrane domain unique to TrkG and TrkH in Escherichia coli. International-journal

Ellen Tanudjaja, Haoyu Zhang, Tadaomi Furuta, Masaru Tsujii, Yasuhiro Ishimaru, Nobuyuki Uozumi

Bioscience, biotechnology, and biochemistry　2025/07/15

DOI： 10.1093/bbb/zbaf101 　

More details Close

Escherichia coli TrkG and TrkH transporters contain a unique N-terminal Domain-0 (D0). Our findings reveal that D0 supports both the function and stability of TrkG, enabling K+ and Na+ uptake, whereas it is not essential for TrkH-mediated K+ uptake. This difference can be attributed to D0 role in stabilizing polar residues within TrkG core transmembrane domains.
Na+-driven pH regulation by Na+/H+ antiporters promotes photosynthetic efficiency in cyanobacteria International-journal

Masaru Tsujii, Ayumu Kobayashi, Ayaka Kano, Kota Kera, Tomoko Takagi, Noriko Nagata, Seiji Kojima, Kouki Hikosaka, Riichi Oguchi, Kintake Sonoike, Chihiro Azai, Tomomi Inagaki, Yasuhiro Ishimaru, Nobuyuki Uozumi

Plant Physiology　197　(1)　2024/10/24
Publisher: Oxford University Press (OUP)
DOI： 10.1093/plphys/kiae562 　

ISSN： 0032-0889

eISSN： 1532-2548

More details Close

Abstract Photosynthetic organisms have developed mechanisms to regulate light reactions in response to varying light conditions. Photosynthetic electron transport leads to the formation of a ΔpH across the thylakoid membrane, which is crucial for regulating electron transport. However, other pH modulators remain to be identified, particularly in cyanobacteria. In this study, we evaluated the potential involvement of six Na+/H+ antiporters (NhaS1–NhaS6) in control of pH in the cyanobacterium Synechocystis sp. PCC 6803. Synechocystis showed a strong requirement for Na+ at high light intensities, with ΔnhaS1 and ΔnhaS2 strains unable to grow under high light conditions. We analyzed Na+ efflux–driven H+-uptake activities of NhaS1–NhaS6 in inverted membranes of Escherichia coli. Biological fractionation and immunoelectron microscopy revealed that NhaS1 localizes to both the plasma and thylakoid membranes while NhaS2 localizes to the plasma membrane. Measurement of photosynthesis activity indicated that NhaS2 promotes ATP production and electron transport from PQ to P700. Measurements of pH outside of the cells and in the cytoplasm suggested that both NhaS1 and NhaS2 are involved in plasma membrane–mediated light-dependent H+ uptake and cytoplasmic acidification. NhaS1 and NhaS2 were also found to prevent photoinhibition under high light treatment. These results indicate that H+ transport mediated by NhaS1 and NhaS2 plays a role in regulating intracellular pH and maintaining photosynthetic electron transport.
Comparison of in-vacuum micro-PIXE and in-air micro-PIXE for unfixed plant sample Peer-reviewed

Misako Miwa, Ayumi Nakatsuma, Shigeo Matsuyama, Sho Toyama, Takeshi Uchiyama, Yasuhiro Ishimaru, Nobuyuki Uozumi

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms　2024/09/28

DOI： 10.1016/j.nimb.2024.165536 　

ISSN： 0168-583X
Dissecting structure and function of the monovalent cation/H ⁺ antiporters Mdm38 and Ylh47 in Saccharomyces cerevisiae International-journal

Masaru Tsujii, Ellen Tanudjaja, Haoyu Zhang, Haruto Shimizukawa, Ayumi Konishi, Tadaomi Furuta, Yasuhiro Ishimaru, Nobuyuki Uozumi

Journal of Bacteriology　206　(8)　e0018224　2024/07/31
Publisher: American Society for Microbiology
DOI： 10.1128/jb.00182-24 　

ISSN： 0021-9193

eISSN： 1098-5530

More details Close

ABSTRACT Saccharomyces cerevisiae Mdm38 and Ylh47 are homologs of the Ca ²⁺ /H ⁺ antiporter Letm1, a candidate gene for seizures associated with Wolf-Hirschhorn syndrome in humans. Mdm38 is important for K ⁺ /H ⁺ exchange across the inner mitochondrial membrane and contributes to membrane potential formation and mitochondrial protein translation. Ylh47 also localizes to the inner mitochondrial membrane. However, knowledge of the structures and detailed transport activities of Mdm38 and Ylh47 is limited. In this study, we conducted characterization of the ion transport activities and related structural properties of Mdm38 and Ylh47. Growth tests using Na ⁺ /H ⁺ antiporter-deficient Escherichia coli strain TO114 showed that Mdm38 and Ylh47 had Na ⁺ efflux activity. Measurement of transport activity across E. coli -inverted membranes showed that Mdm38 and Ylh47 had K ⁺ /H ⁺ , Na ⁺ /H ⁺ , and Li ⁺ /H ⁺ antiport activity, but unlike Letm1, they lacked Ca ²⁺ /H ⁺ antiport activity. Deletion of the ribosome-binding domain resulted in decreased Na ⁺ efflux activity in Mdm38. Structural models of Mdm38 and Ylh47 identified a highly conserved glutamic acid in the pore-forming membrane-spanning region. Replacement of this glutamic acid with alanine, a non-polar amino acid, significantly impaired the ability of Mdm38 and Ylh47 to complement the salt sensitivity of E. coli TO114. These findings not only provide important insights into the structure and function of the Letm1-Mdm38-Ylh47 antiporter family but by revealing their distinctive properties also shed light on the physiological roles of these transporters in yeast and animals. IMPORTANCE The inner membrane of mitochondria contains numerous ion transporters, including those facilitating H ⁺ transport by the electron transport chain and ATP synthase to maintain membrane potential. Letm1 in the inner membrane of mitochondria in animals functions as a Ca ²⁺ /H ⁺ antiporter. However, this study reveals that homologous antiporters in mitochondria of yeast, Mdm38 and Ylh47, do not transport Ca ²⁺ but instead are selective for K ⁺ and Na ⁺ . Additionally, the identification of conserved amino acids crucial for antiporter activity further expanded our understanding of the structure and function of the Letm1-Mdm38-Ylh47 antiporter family.
Utilizing plasma-generated N2O5 gas from atmospheric air as a novel gaseous nitrogen source for plants. International-journal

Taro Yamanashi, Shouki Takeshi, Shota Sasaki, Keisuke Takashima, Toshiro Kaneko, Yasuhiro Ishimaru, Nobuyuki Uozumi

Plant molecular biology　114　(2)　35-35　2024/04/08

DOI： 10.1007/s11103-024-01438-9 　

More details Close

Fixing atmospheric nitrogen for use as fertilizer is a crucial process in promoting plant growth and enhancing crop yields in agricultural production. Currently, the chemical production of nitrogen fertilizer from atmospheric N2 relies on the energy-intensive Haber-Bosch process. Therefore, developing a low-cost and easily applicable method for fixing nitrogen from the air would provide a beneficial alternative. In this study, we tested the utilization of dinitrogen pentoxide (N2O5) gas, generated from oxygen and nitrogen present in ambient air with the help of a portable plasma device, as a nitrogen source for the model plant Arabidopsis thaliana. Nitrogen-deficient plants supplied with medium treated with N2O5, were able to overcome nitrogen deficiency, similar to those provided with medium containing a conventional nitrogen source. However, prolonged direct exposure of plants to N2O5 gas adversely affected their growth. Short-time exposure of plants to N2O5 gas mitigated its toxicity and was able to support growth. Moreover, when the exposure of N2O5 and the contact with plants were physically separated, plants cultured under nitrogen deficiency were able to grow. This study shows that N2O5 gas generated from atmospheric nitrogen can be used as an effective nutrient for plants, indicating its potential to serve as an alternative nitrogen fertilization method for promoting plant growth.
An outward-rectifying plant K+ channel SPORK2 exhibits temperature-sensitive ion-transport activity. International-journal

Yuki Muraoka, Gangqiang Yang, Shintaro Munemasa, Yusuke Takeuchi, Yasuhiro Ishimaru, Yoshiyuki Murata, Nobuyuki Uozumi, Minoru Ueda

Current biology : CB　33　(24)　5488-5494　2023/11/17

DOI： 10.1016/j.cub.2023.10.057 　

More details Close

Temperature sensing is critical for the survival of living organisms.1,2 Thermosensitive transient receptor-potential (TRP) cation channels function as thermosensors in mammals.2,3,4,5,6 In contrast to animals, land plants lack TRP genes.7,8,9 Previous patch-clamp studies in plant cells suggested the presence of ion channels whose activities are related to temperature, implying the presence of TRP-like channels.10,11,12,13,14 However, the molecular entities of such temperature-sensitive ion channels were still unknown in land plants. In this study, we observed that the unique rainfall-induced leaf-folding movement of the legume tree Samanea saman15 was temperature-sensitive by using a rainfall-mimicking assay. Chilling-induced leaf folding in S. saman was shown to be related to the swelling of the motor cells16,17 at the base of the leaflet. This swelling suggested involvement of temperature-sensitive inactivation of K+ currents, independent of fluctuations in ion channel gene expression in motor cells. These findings led us to examine the temperature sensitivity of an outward-rectifying K+ channel, SPORK2, which was reported as an ion channel responsible for the nyctinastic (circadian-rhythmic) leaf movement of S. saman.18 We also discovered that SPORK2 exhibits temperature-sensitive K+ transport activity in the Xenopus oocyte expression system. Using chimeric channels, we showed that two domains of SPORK2 regulated the temperature sensitivity. Furthermore, heterologously expressed SPORK2 in Arabidopsis guard cells induced temperature-dependent stomatal closure. Therefore, SPORK2 is an ion channel in land plants with temperature-sensitive ion-transport activity that functions similarly to mammalian TRP channels. Our current findings advance the molecular understanding of temperature-sensing mechanisms in plants.
The HKT1 Na+ transporter protects plant fertility by decreasing Na+ content in stamen filaments. International-journal

Takeshi Uchiyama, Shunya Saito, Taro Yamanashi, Megumi Kato, Kosuke Takebayashi, Shin Hamamoto, Masaru Tsujii, Tomoko Takagi, Noriko Nagata, Hayato Ikeda, Hidetoshi Kikunaga, Toshimi Suda, Sho Toyama, Misako Miwa, Shigeo Matsuyama, Mitsunori Seo, Tomoaki Horie, Takashi Kuromori, Mutsumi Yamagami, Yasuhiro Ishimaru, Nobuyuki Uozumi

Science advances　9　(22)　eadg5495　2023/06/02

DOI： 10.1126/sciadv.adg5495 　

More details Close

Salinity stress can greatly reduce seed production because plants are especially sensitive to salt during their reproductive stage. Here, we show that the sodium ion transporter AtHKT1;1 is specifically expressed around the phloem and xylem of the stamen in Arabidopsis thaliana to prevent a marked decrease in seed production caused by salt stress. The stamens of AtHKT1;1 mutant under salt stress overaccumulate Na+, limiting their elongation and resulting in male sterility. Specifically restricting AtHKT1;1 expression to the phloem leads to a 1.5-fold increase in the seed yield upon sodium ion stress. Expanding phloem expression of AtHKT1;1 throughout the entire plant is a promising strategy for increasing plant productivity under salinity stress.
Two cyanobacterial response regulators with diguanylate cyclase activity, Rre2 and Rre8, participate in biofilm formation International-journal

Ayumu Kobayashi, Masamune Nakamura, Masaru Tsujii, Kohei Makino, Tatsuya Nagayama, Kensuke Nakamura, Kei Nanatani, Kera Kota, Yuki Furuuchi, Shunsuke Kayamori, Tadaomi Furuta, Iwane Suzuki, Yoshihiro Hayakawa, Tanudjaja Ellen, Yasuhiro Ishimaru, Nobuyuki Uozumi

Molecular Microbiology　119　(5)　599-611　2023/03
Publisher: Wiley
DOI： 10.1111/mmi.15057 　

ISSN： 0950-382X

eISSN： 1365-2958

More details Close

Phototrophic bacteria face diurnal variations of environmental conditions such as light and osmolarity, that affect their carbon metabolism and ability to generate organic compounds. The model cyanobacterium, Synechocystis sp. PCC 6803 forms a biofilm when it encounters extreme conditions like high salt stress, but the molecular mechanisms involved in perception of environmental changes that lead to biofilm formation are unknown. Here, we studied two two-component regulatory systems (TCSs) that contain diguanylate cyclases (DGCs), which produce the second messenger c-di-GMP, as potential components of the biofilm-inducing signaling pathway in Synechocystis. Analysis of single mutants provided evidence for involvement of Rre2 and Rre8 in biofilm formation. A bacterial two-hybrid assay showed that the response regulators, Rre2 and Rre8 each formed a TCS with a specific histidine kinase, Hik12 and Hik14, respectively. The in vitro assay showed that Rre2 had DGC activity regardless of its de/phosphorylation status, whereas Rre8 required phosphorylation for DGC activity. Hik14-Rre8 likely functioned as an inducible sensing system in response to environmental change. Biofilm assays with Synechocystis mutants suggested that pairs of hik12-rre2 and hik14-rre8 responded to high salinity-induced biofilm formation. Inactivation of hik12-rre2 and hik14-rre8 did not affect the performance of the light reactions of photosynthesis. These data suggest that Hik12-Rre2 and Hik14-Rre8 participate in biofilm formation in Synechocystis by regulating c-di-GMP production via the DGC activity of Rre2 and Rre8.
Potassium transporter KUP9 participates in K+ distribution in roots and leaves under low K+ stress. International-journal

Taro Yamanashi, Takeshi Uchiyama, Shunya Saito, Taiki Higashi, Hayato Ikeda, Hidetoshi Kikunaga, Mutsumi Yamagami, Yasuhiro Ishimaru, Nobuyuki Uozumi

Stress biology　2　(1)　52-52　2022/12/12
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s44154-022-00074-x 　

eISSN： 2731-0450

More details Close

Abstract Potassium (K) is a major essential element in plant cells, and KUP/HAK/KT-type K⁺ transporters participate in the absorption of K⁺ into roots and in the long-distance transport to above-ground parts. In Arabidopsis thaliana, KUP9 is involved in the transport of K⁺ and Cs⁺ in roots. In this study, we investigated KUP9 function in relation to the K⁺ status of the plant. The expression of KUP9 was upregulated in older leaves on K⁺-depleted medium, compared to the expression of the other 12 KUP genes in the KUP/HAK/KT family in Arabidopsis. When grown on low K⁺ medium, the kup9 mutant had reduced chlorophyll content in seedlings and chlorosis in older rosette leaves. Tissue-specific expression of KUP9 determined by KUP9 promoter:GUS assay depended on the K⁺ status of the plants: In K⁺ sufficient medium, KUP9 was expressed in the leaf blade towards the leaf tip, whereas in K⁺ depleted medium expression was mainly found in the petioles. In accordance with this, K⁺ accumulated in the roots of kup9 plants. The short-term ⁴³K⁺ tracer measurement showed that ⁴³K was transferred at a lower rate in roots and shoots of kup9, compared to the wild type. These data show that KUP9 participates in the distribution of K⁺ in leaves and K⁺ absorption in roots under low K⁺ conditions.
Two Trk/Ktr/HKT-type potassium transporters, TrkG and TrkH perform distinct functions in Escherichia coli K-12 International-journal

Ellen Tanudjaja, Naomi Hoshi, Kaneyoshi Yamamoto, Kunio Ihara, Tadaomi Furuta, Masaru Tsujii, Yasuhiro Ishimaru, Nobuyuki Uozumi

Journal of Biological Chemistry　299　(2)　102846-102846　2022/12
Publisher: Elsevier BV
DOI： 10.1016/j.jbc.2022.102846 　

ISSN： 0021-9258

More details Close

Escherichia coli K-12 possesses two versions of Trk/Ktr/HKT-type potassium ion (K+) transporters, TrkG and TrkH. The current paradigm is that TrkG and TrkH have largely identical characteristics, and little information is available regarding their functional differences. Here, we show using cation uptake experiments with K+ transporter knockout mutants that TrkG and TrkH have distinct ion transport activities and physiological roles. K+-transport by TrkG required Na+, whereas TrkH-mediated K+ uptake was not affected by Na+. An aspartic acid located five residues away from a critical glycine in the third pore-forming region might be involved in regulation of Na+-dependent activation of TrkG. In addition, we found that TrkG but not TrkH had Na+ uptake activity. Our analysis of K+ transport mutants revealed that TrkH supported cell growth more than TrkG; however, TrkG was able to complement loss of TrkH-mediated K+ uptake in E. coli. Furthermore, we determined that transcription of trkG in E. coli was downregulated but not completely silenced by the xenogeneic silencing factor H-NS (histone-like nucleoid structuring protein or heat-stable nucleoid-structuring protein). Taken together, the transport function of TrkG is clearly distinct from that of TrkH, and TrkG seems to have been accepted by E. coli during evolution as a K+ uptake system that coexists with TrkH.
Green Tea Catechins, (-)-Catechin Gallate, and (-)-Gallocatechin Gallate are Potent Inhibitors of ABA-Induced Stomatal Closure. International-journal Peer-reviewed

Kanane Sato, Shunya Saito, Kohsuke Endo, Masaru Kono, Taishin Kakei, Haruka Taketa, Megumi Kato, Shin Hamamoto, Matteo Grenzi, Alex Costa, Shintaro Munemasa, Yoshiyuki Murata, Yasuhiro Ishimaru, Nobuyuki Uozumi

Advanced science (Weinheim, Baden-Wurttemberg, Germany)　9　(21)　e2201403　2022/05/07

DOI： 10.1002/advs.202201403 　

More details Close

Stomatal movement is indispensable for plant growth and survival in response to environmental stimuli. Cytosolic Ca2+ elevation plays a crucial role in ABA-induced stomatal closure during drought stress; however, to what extent the Ca2+ movement across the plasma membrane from the apoplast to the cytosol contributes to this process still needs clarification. Here the authors identify (-)-catechin gallate (CG) and (-)-gallocatechin gallate (GCG), components of green tea, as inhibitors of voltage-dependent K+ channels which regulate K+ fluxes in Arabidopsis thaliana guard cells. In Arabidopsis guard cells CG/GCG prevent ABA-induced: i) membrane depolarization; ii) activation of Ca2+ permeable cation (ICa ) channels; and iii) cytosolic Ca2+ transients. In whole Arabidopsis plants co-treatment with CG/GCG and ABA suppressed ABA-induced stomatal closure and surface temperature increase. Similar to ABA, CG/GCG inhibited stomatal closure is elicited by the elicitor peptide, flg22 but has no impact on dark-induced stomatal closure or light- and fusicoccin-induced stomatal opening, suggesting that the inhibitory effect of CG/GCG is associated with Ca2+ -related signaling pathways. This study further supports the crucial role of ICa channels of the plasma membrane in ABA-induced stomatal closure. Moreover, CG and GCG represent a new tool for the study of abiotic or biotic stress-induced signal transduction pathways.
12-Hydroxyjasmonic acid glucoside causes leaf-folding of Samanea saman through ROS accumulation. International-journal Peer-reviewed

Gangqiang Yang, Yasuhiro Ishimaru, Shunji Hoshino, Yuki Muraoka, Nobuyuki Uozumi, Minoru Ueda

Scientific reports　12　(1)　7232-7232　2022/05/04

DOI： 10.1038/s41598-022-11414-2 　

More details Close

Foliar nyctinasty, a circadian rhythmic movement in plants, is common among leguminous plants and has been widely studied. Biological studies on nyctinasty have been conducted using Samanea saman as a model plant. It has been shown that the circadian rhythmic potassium flux from/into motor cells triggers cell shrinking/swelling to cause nyctinastic leaf-folding/opening movement in S. saman. Recently, 12-hydroxyjasmonic acid glucoside (JAG) was identified as an endogenous chemical factor causing leaf-folding of S. saman. Additionally, SPORK2 was identified as an outward-rectifying potassium channel that causes leaf-movement in the same plant. However, the molecular mechanism linking JAG and SPORK2 remains elusive. Here, we report that JAG induces leaf-folding through accumulation of reactive oxygen species in the extensor motor cells of S. saman, and this occurs independently of plant hormone signaling. Furthermore, we show that SPORK2 is indispensable for the JAG-triggered shrinkage of the motor cell. This is the first report on JAG, which is believed to be an inactivated/storage derivative of JA, acting as a bioactive metabolite in plant.
Challenges and opportunities to regulate mineral transport in rice. International-journal Peer-reviewed

Khurram Bashir, Yasuhiro Ishimaru

Bioscience, biotechnology, and biochemistry　86　(1)　12-22　2021/12/22

DOI： 10.1093/bbb/zbab180 　

More details Close

Iron (Fe) is an essential mineral for plants, and its deficiency as well as toxicity severely affects plant growth and development. Although Fe is ubiquitous in mineral soils, its acquisition by plants is difficult to regulate particularly in acidic and alkaline soils. Under alkaline conditions, where lime is abundant, Fe and other mineral elements are sparingly soluble. In contrast, under low pH conditions, especially in paddy fields, Fe toxicity could occur. Fe uptake is complicated and could be integrated with copper (Cu), manganese (Mn), zinc (Zn), and cadmium (Cd) uptake. Plants have developed sophisticated mechanisms to regulate the Fe uptake from soil and its transport to root and above-ground parts. Here, we review recent developments in understanding metal transport and discuss strategies to effectively regulate metal transport in plants with a particular focus on rice.
Plant nyctinasty - who will decode the 'Rosetta Stone'? International-journal Peer-reviewed

Minoru Ueda, Yasuhiro Ishimaru, Yusuke Takeuchi, Yuki Muraoka

The New phytologist　223　(1)　107-112　2019/07

DOI： 10.1111/nph.15717 　

ISSN： 0028-646X

More details Close

Nyctinasty is the circadian rhythmic nastic movement of leguminous plants in response to the onset of darkness, a unique and intriguing phenomenon that has attracted attention for centuries. The movement itself is caused by the asymmetric volume change of motor cells between the adaxial and abaxial sides of the leaflet. Recently, we identified the ion channels responsible for the volume change of motor cells during the leaf-opening process of Samanea saman; the asymmetric expression of SsSLAH1, which is under the control of SsCCA1, was found to play a key role in this process. Here, we summarize the history of the study of nyctinasty, our current results and several insights for further study.
A rationally designed JAZ subtype-selective agonist of jasmonate perception. International-journal Peer-reviewed

Takaoka Y, Iwahashi M, Chini A, Saito H, Ishimaru Y, Egoshi S, Kato N, Tanaka M, Bashir K, Seki M, Solano R, Ueda M

Nature communications　9　(1)　3654-3654　2018/09

DOI： 10.1038/s41467-018-06135-y 　

ISSN： 2041-1723
Jasmonic Acid Inhibits Auxin-Induced Lateral Rooting Independently of the CORONATINE INSENSITIVE1 Receptor. International-journal Peer-reviewed

Yasuhiro Ishimaru, Kengo Hayashi, Takeshi Suzuki, Hidehiro Fukaki, Justyna Prusinska, Christian Meester, Mussa Quareshy, Syusuke Egoshi, Hideyuki Matsuura, Kosaku Takahashi, Nobuki Kato, Erich Kombrink, Richard M Napier, Ken-Ichiro Hayashi, Minoru Ueda

Plant physiology　177　(4)　1704-1716　2018/08

DOI： 10.1104/pp.18.00357 　

ISSN： 0032-0889
Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman International-journal Peer-reviewed

Takaya Oikawa, Yasuhiro Ishimaru, Shintaro Munemasa, Yusuke Takeuchi, Kento Washiyama, Shin Hamamoto, Nobuyuki Yoshikawa, Yoshiyuki Mutara, Nobuyuki Uozumi, Minoru Ueda

Current Biology　28　(14)　2230-2238.e7　2018
Publisher: Cell Press
DOI： 10.1016/j.cub.2018.05.042 　

ISSN： 0960-9822
The alkyne-tag Raman imaging of coronatine, a plant pathogen virulence factor, in: Commelina communis and its possible mode of action International-journal Peer-reviewed

Minoru Ueda, Kengo Hayashi, Syusuke Egoshi, Yasuhiro Ishimaru, Yousuke Takaoka, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka

Organic and Biomolecular Chemistry　16　(18)　3348-3352　2018

DOI： 10.1039/c8ob00097b 　

ISSN： 1477-0520
Dimerization of GTR1 regulates their plasma membrane localization International-journal Peer-reviewed

Yasuhiro Ishimaru, Kento Washiyama, Takaya Oikawa, Shin Hamamoto, Nobuyuki Uozumi, Minoru Ueda

Plant Signaling and Behavior　12　(6)　e1334749　2017/06/03

DOI： 10.1080/15592324.2017.1334749 　

ISSN： 1559-2324 1559-2316
Noncanonical Function of a Small-Molecular Virulence Factor Coronatine against Plant Immunity: An In Vivo Raman Imaging Approach International-journal Peer-reviewed

Minoru Ueda, Syusuke Egoshi, Kosuke Dodo, Yasuhiro Ishimaru, Hiroyuki Yamakoshi, Takeshi Nakano, Yousuke Takaoka, Shinya Tsukiji, Mikiko Sodeoka

ACS CENTRAL SCIENCE　3　(5)　462-472　2017/05

DOI： 10.1021/acscentsci.7b00099 　

ISSN： 2374-7943

eISSN： 2374-7951
A new transgenic rice line exhibiting enhanced ferric iron reduction and phytosiderophore production confers tolerance to low iron availability in calcareous soil International-journal Peer-reviewed

Hiroshi Masuda, Erika Shimochi, Tatsuro Hamada, Takeshi Senoura, Takanori Kobayashi, May Sann Aung, Yasuhiro Ishimaru, Yuko Ogo, Hiromi Nakanishi, Naoko K. Nishizawa

PLOS ONE　12　(3)　e0173441　2017/03

DOI： 10.1371/journal.pone.0173441 　

ISSN： 1932-6203
GTR1 is a jasmonic acid and jasmonoyl-L-isoleucine transporter in Arabidopsis thaliana International-journal Peer-reviewed

Yasuhiro Ishimaru, Takaya Oikawa, Takeshi Suzuki, Syohei Takeishi, Hideyuki Matsuura, Kosaku Takahashi, Shin Hamamoto, Nobuyuki Uozumi, Takafumi Shimizu, Mitsunori Seo, Hiroyuki Ohta, Minoru Ueda

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　81　(2)　249-255　2017/02

DOI： 10.1080/09168451.2016.1246174 　

ISSN： 0916-8451

eISSN： 1347-6947
AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes International-journal Peer-reviewed

Yuri Kanno, Takaya Oikawa, Yasutaka Chiba, Yasuhiro Ishimaru, Takafumi Shimizu, Naoto Sano, Tomokazu Koshiba, Yuji Kamiya, Minoru Ueda, Mitsunori Seo

NATURE COMMUNICATIONS　7　13245-13245　2016/10

DOI： 10.1038/ncomms13245 　

ISSN： 2041-1723
Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots International-journal Peer-reviewed

Takanori Kobayashi, Reiko Nakanishi Itai, Takeshi Senoura, Takaya Oikawa, Yasuhiro Ishimaru, Minoru Ueda, Hiromi Nakanishi, Naoko K. Nishizawa

PLANT MOLECULAR BIOLOGY　91　(4-5)　533-547　2016/07

DOI： 10.1007/s11103-016-0486-3 　

ISSN： 0167-4412

eISSN： 1573-5028
Knocking down mitochondrial iron transporter (MIT) reprograms primary and secondary metabolism in rice plants International-journal Peer-reviewed

Gianpiero Vigani, Khurram Bashir, Yasuhiro Ishimaru, Martin Lehmann, Fabio Marco Casiraghi, Hiromi Nakanishi, Motoaki Seki, Peter Geigenberger, Graziano Zocchi, Naoko K. Nishizawa

JOURNAL OF EXPERIMENTAL BOTANY　67　(5)　1357-1368　2016/03

DOI： 10.1093/jxb/erv531 　

ISSN： 0022-0957

eISSN： 1460-2431
Protein ligand-tethered synthetic calcium indicator for localization control and spatiotemporal calcium imaging in plant cells International-journal Peer-reviewed

Yousuke Takaoka, Miyuki Shigenaga, Masaki Imai, Yuuki Nukadzuka, Yasuhiro Ishimaru, Kei Saito, Ryusuke Yokoyama, Kazuhiko Nishitani, Minoru Ueda

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　26　(1)　9-14　2016/01

DOI： 10.1016/j.bmcl.2015.11.055 　

ISSN： 0960-894X

eISSN： 1464-3405
Dual function of coronatine as a bacterial virulence factor against plants: possible COI1-JAZ-independent role Peer-reviewed

Syusuke Egoshi, Yousuke Takaoka, Hiroaki Saito, Yuuki Nukadzuka, Kengo Hayashi, Yasuhiro Ishimaru, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka, Minoru Ueda

RSC ADVANCES　6　(23)　19404-19412　2016

DOI： 10.1039/c5ra20676f 　

ISSN： 2046-2069
Iron deficiency regulated OsOPT7 is essential for iron homeostasis in rice International-journal Peer-reviewed

Khurram Bashir, Yasuhiro Ishimaru, Reiko Nakanishi Itai, Takeshi Senoura, Michiko Takahashi, Gynheung An, Takaya Oikawa, Minoru Ueda, Aiko Sato, Nobuyuki Uozumi, Hiromi Nakanishi, Naoko K. Nishizawa

PLANT MOLECULAR BIOLOGY　88　(1-2)　165-176　2015/05

DOI： 10.1007/s11103-015-0315-0 　

ISSN： 0167-4412

eISSN： 1573-5028
Elevated Levels of CYP94 Family Gene Expression Alleviate the Jasmonate Response and Enhance Salt Tolerance in Rice Peer-reviewed

Ken-ichi Kurotani, Kenji Hayashi, Saki Hatanaka, Yosuke Toda, Daisuke Ogawa, Hiroaki Ichikawa, Yasuhiro Ishimaru, Ryo Tashita, Takeshi Suzuki, Minoru Ueda, Tsukaho Hattori, Shin Takeda

PLANT AND CELL PHYSIOLOGY　56　(4)　779-789　2015/04

DOI： 10.1093/pcp/pcv006 　

ISSN： 0032-0781

eISSN： 1471-9053
The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis International-journal Peer-reviewed

Hikaru Saito, Takaya Oikawa, Shin Hamamoto, Yasuhiro Ishimaru, Miyu Kanamori-Sato, Yuko Sasaki-Sekimoto, Tomoya Utsumi, Jing Chen, Yuri Kanno, Shinji Masuda, Yuji Kamiya, Mitsunori Seo, Nobuyuki Uozumi, Minoru Ueda, Hiroyuki Ohta

NATURE COMMUNICATIONS　6　6095-6095　2015/02

DOI： 10.1038/ncomms7095 　

ISSN： 2041-1723
Functional importance of the sugar moiety of jasmonic acid glucoside for bioactivity and target affinity International-journal Peer-reviewed

Minoru Ueda, Gangqiang Yang, Yuuki Nukadzuka, Yasuhiro Ishimaru, Satoru Tamura, Yoshiyuki Manabe

ORGANIC & BIOMOLECULAR CHEMISTRY　13　(1)　55-58　2015/01

DOI： 10.1039/c4ob02106a 　

ISSN： 1477-0520

eISSN： 1477-0539
From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields International-journal Peer-reviewed

Ryuichi Takahashi, Yasuhiro Ishimaru, Hugo Shimo, Khurram Bashir, Takeshi Senoura, Kazuhiko Sugimoto, Kazuko Ono, Nobuo Suzui, Naoki Kawachi, Satomi Ishii, Yong-Gen Yin, Shu Fujimaki, Masahiro Yano, Naoko K. Nishizawa, Hiromi Nakanishi

PLOS ONE　9　(6)　e98816　2014/06

DOI： 10.1371/journal.pone.0098816 　

ISSN： 1932-6203
Exploiting new tools for iron bio-fortification of rice International-journal Peer-reviewed

Khurram Bashir, Tomoko Nozoye, Yasuhiro Ishimaru, Hiromi Nakanishi, Naoko K. Nishizawa

BIOTECHNOLOGY ADVANCES　31　(8)　1624-1633　2013/12

DOI： 10.1016/j.biotechadv.2013.08.012 　

ISSN： 0734-9750

eISSN： 1873-1899
Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene International-journal Peer-reviewed

Hiroshi Masuda, Takanori Kobayashi, Yasuhiro Ishimaru, Michiko Takahashi, May S. Aung, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

FRONTIERS IN PLANT SCIENCE　4　132-132　2013/05

DOI： 10.3389/fpls.2013.00132 　

ISSN： 1664-462X
The knockdown of OsVIT2 and MIT affects iron localization in rice seed International-journal Peer-reviewed

Khurram Bashir, Ryuichi Takahashi, Shamim Akhtar, Yasuhiro Ishimaru, Hiromi Nakanishi, Naoko K. Nishizawa

Rice　6　(1)　1-6　2013

DOI： 10.1186/1939-8433-6-31 　

ISSN： 1939-8425 1939-8433
Molecular mechanisms of zinc uptake and translocation in rice Peer-reviewed

Khurram Bashir, Yasuhiro Ishimaru, Naoko K. Nishizawa

PLANT AND SOIL　361　(1-2)　189-201　2012/12

DOI： 10.1007/s11104-012-1240-5 　

ISSN： 0032-079X
The OsHMA2 transporter is involved in root-to-shoot translocation of Zn and Cd in rice International-journal Peer-reviewed

Ryuichi Takahashi, Yasuhiro Ishimaru, Hugo Shimo, Yuko Ogo, Takeshi Senoura, Naoko K. Nishizawa, Hiromi Nakanishi

PLANT CELL AND ENVIRONMENT　35　(11)　1948-1957　2012/11

DOI： 10.1111/j.1365-3040.2012.02527.x 　

ISSN： 0140-7791
Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice International-journal Peer-reviewed

Satoru Ishikawa, Yasuhiro Ishimaru, Masato Igura, Masato Kuramata, Tadashi Abe, Takeshi Senoura, Yoshihiro Hase, Tomohito Arao, Naoko K. Nishizawa, Hiromi Nakanishi

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　109　(47)　19166-19171　2012/11

DOI： 10.1073/pnas.1211132109 　

ISSN： 0027-8424
Hybrid stereoisomers of a compact molecular probe based on a jasmonic acid glucoside: Syntheses and biological evaluations International-journal Peer-reviewed

Minoru Ueda, Gangqiang Yang, Yasuhiro Ishimaru, Tetsuya Itabashi, Satoru Tamura, Hiromasa Kiyota, Shigefumi Kuwahara, Sho Inomata, Mitsuru Shoji, Takeshi Sugai

BIOORGANIC & MEDICINAL CHEMISTRY　20　(19)　5832-5843　2012/10

DOI： 10.1016/j.bmc.2012.08.003 　

ISSN： 0968-0896
OsYSL16 plays a role in the allocation of iron International-journal Peer-reviewed

Yusuke Kakei, Yasuhiro Ishimaru, Takanori Kobayashi, Takashi Yamakawa, Hiromi Nakanishi, Naoko K. Nishizawa

PLANT MOLECULAR BIOLOGY　79　(6)　583-594　2012/08

DOI： 10.1007/s11103-012-9930-1 　

ISSN： 0167-4412
Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition International-journal Peer-reviewed

Hiroshi Masuda, Yasuhiro Ishimaru, May Sann Aung, Takanori Kobayashi, Yusuke Kakei, Michiko Takahashi, Kyoko Higuchi, Hiromi Nakanishi, Naoko K. Nishizawa

SCIENTIFIC REPORTS　2　543-543　2012/07

DOI： 10.1038/srep00543 　

ISSN： 2045-2322
Regulatory mechanism of plant nyctinastic movement: An ion channel-related plant behavior Peer-reviewed

Yasuhiro Ishimaru, Shin Hamamoto, Nobuyuki Uozumi, Minoru Ueda

Plant Electrophysiology: Signaling and Responses　125-142　2012/02/01
Publisher: Springer-Verlag Berlin Heidelberg
DOI： 10.1007/978-3-642-29110-4_5 　
Characterizing the role of rice NRAMP5 in Manganese, Iron and Cadmium Transport International-journal Peer-reviewed

Yasuhiro Ishimaru, Ryuichi Takahashi, Khurram Bashir, Hugo Shimo, Takeshi Senoura, Kazuhiko Sugimoto, Kazuko Ono, Masahiro Yano, Satoru Ishikawa, Tomohito Arao, Hiromi Nakanishi, Naoko K. Nishizawa

SCIENTIFIC REPORTS　2　286-286　2012/02

DOI： 10.1038/srep00286 　

ISSN： 2045-2322
OsNRAMP5, a major player for constitutive iron and manganese uptake in rice International-journal Peer-reviewed

Yasuhiro Ishimaru, Khurram Bashir, Hiromi Nakanishi, Naoko K. Nishizawa

Plant Signaling and Behavior　7　(7)　1-4　2012
Publisher: Landes Bioscience
DOI： 10.4161/psb.20510 　

ISSN： 1559-2324 1559-2316
The role of heavy-metal ATPases, HMAs, in zinc and cadmium transport in rice International-journal Peer-reviewed

Ryuichi Takahashi, Khurram Bashir, Yasuhiro Ishimaru, Naoko K. Nishizawa, Hiromi Nakanishi

Plant Signaling and Behavior　7　(12)　1605-1607　2012
Publisher: Landes Bioscience
DOI： 10.4161/psb.22454 　

ISSN： 1559-2324 1559-2316
Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron Peer-reviewed

Khurram Bashir, Yasuhiro Ishimaru, Hugo Shimo, Yusuke Kakei, Takeshi Senoura, Ryuichi Takahashi, Yutaka Sato, Yuki Sato, Nobuyuki Uozumi, Hiromi Nakanishi, Naoko K. Nishizawa

SOIL SCIENCE AND PLANT NUTRITION　57　(6)　803-812　2011/12

DOI： 10.1080/00380768.2011.637305 　

ISSN： 0038-0768
Role of the iron transporter OsNRAMP1 in cadmium uptake and accumulation in rice International-journal Peer-reviewed

Ryuichi Takahashi, Yasuhiro Ishimaru, Hiromi Nakanishi, Naoko K. Nishizawa

Plant Signaling and Behavior　6　(11)　1813-1816　2011/11

DOI： 10.4161/psb.6.11.17587 　

ISSN： 1559-2316 1559-2324
Low cadmium (LCD), a novel gene related to cadmium tolerance and accumulation in rice International-journal Peer-reviewed

Hugo Shimo, Yasuhiro Ishimaru, Gynheung An, Takashi Yamakawa, Hiromi Nakanishi, Naoko K. Nishizawa

JOURNAL OF EXPERIMENTAL BOTANY　62　(15)　5727-5734　2011/11

DOI： 10.1093/jxb/err300 　

ISSN： 0022-0957
Identification and characterization of the major mitochondrial Fe transporter in rice International-journal Peer-reviewed

Khurram Bashir, Yasuhiro Ishimaru, Naoko K. Nishizawa

Plant Signaling and Behavior　6　(10)　1591-1593　2011/10

DOI： 10.4161/psb.6.10.17132 　

ISSN： 1559-2316 1559-2324
The OsNRAMP1 iron transporter is involved in Cd accumulation in rice International-journal Peer-reviewed

Ryuichi Takahashi, Yasuhiro Ishimaru, Takeshi Senoura, Hugo Shimo, Satoru Ishikawa, Tomohito Arao, Hiromi Nakanishi, Naoko K. Nishizawa

JOURNAL OF EXPERIMENTAL BOTANY　62　(14)　4843-4850　2011/10

DOI： 10.1093/jxb/err136 　

ISSN： 0022-0957
A Rice Phenolic Efflux Transporter Is Essential for Solubilizing Precipitated Apoplasmic Iron in the Plant Stele International-journal Peer-reviewed

Yasuhiro Ishimaru, Yusuke Kakei, Hugo Shimo, Khurram Bashir, Yutaka Sato, Yuki Sato, Nobuyuki Uozumi, Hiromi Nakanishi, Naoko K. Nishizawa

JOURNAL OF BIOLOGICAL CHEMISTRY　286　(28)　24649-24655　2011/07

DOI： 10.1074/jbc.M111.221168 　

ISSN： 0021-9258
The rice mitochondrial iron transporter is essential for plant growth International-journal Peer-reviewed

Khurram Bashir, Yasuhiro Ishimaru, Hugo Shimo, Seiji Nagasaka, Masaru Fujimoto, Hideki Takanashi, Nobuhiro Tsutsumi, Gynheung An, Hiromi Nakanishi, Naoko K. Nishizawa

NATURE COMMUNICATIONS　2　322-322　2011/05

DOI： 10.1038/ncomms1326 　

ISSN： 2041-1723
Zn Uptake and Translocation in Rice Plants Peer-reviewed

Yasuhiro Ishimaru, Khurram Bashir, Naoko K. Nishizawa

RICE　4　(1)　21-27　2011/03

DOI： 10.1007/s12284-011-9061-3 　

ISSN： 1939-8425
The role of rice phenolics efflux transporter in solubilizing apoplasmic iron International-journal Peer-reviewed

Yasuhiro Ishimaru, Khurram Bashir, Hiromi Nakanishi, Naoko K. Nishizawa

Plant Signaling and Behavior　6　(10)　1624-1626　2011
Publisher: Landes Bioscience
DOI： 10.4161/psb.6.10.17694 　

ISSN： 1559-2324 1559-2316
Iron Uptake and Loading into Rice Grains Peer-reviewed

Khurram Bashir, Yasuhiro Ishimaru, Naoko K. Nishizawa

RICE　3　(2-3)　122-130　2010/09

DOI： 10.1007/s12284-010-9042-y 　

ISSN： 1939-8425
Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese International-journal Peer-reviewed

Yasuhiro Ishimaru, Hiroshi Masuda, Khurram Bashir, Haruhiko Inoue, Takashi Tsukamoto, Michiko Takahashi, Hiromi Nakanishi, Naohiro Aoki, Tatsuro Hirose, Ryu Ohsugi, Naoko K. Nishizawa

PLANT JOURNAL　62　(3)　379-390　2010/05

DOI： 10.1111/j.1365-313X.2010.04158.x 　

ISSN： 0960-7412

eISSN： 1365-313X
In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu Peer-reviewed

Michiko Takahashi, Tomoko Nozoye, Nobuyuki Kitajima, Naoki Fukuda, Akiko Hokura, Yasuko Terada, Izumi Nakai, Yasuhiro Ishimaru, Takanori Kobayashi, Hiromi Nakanishi, Naoko K. Nishizawa

PLANT AND SOIL　325　(1-2)　39-51　2009/12

DOI： 10.1007/s11104-009-0045-7 　

ISSN： 0032-079X

eISSN： 1573-5036
Overexpression of the Barley Nicotianamine Synthase Gene HvNAS1 Increases Iron and Zinc Concentrations in Rice Grains Peer-reviewed

Hiroshi Masuda, Kanako Usuda, Takanori Kobayashi, Yasuhiro Ishimaru, Yusuke Kakei, Michiko Takahashi, Kyoko Higuchi, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

RICE　2　(4)　155-166　2009/12

DOI： 10.1007/s12284-009-9031-1 　

ISSN： 1939-8425

eISSN： 1939-8433
Rice-Specific Mitochondrial Iron-Regulated Gene (MIR) Plays an Important Role in Iron Homeostasis International-journal Peer-reviewed

Yasuhiro Ishimaru, Khurram Bashir, Masaru Fujimoto, Gynheung An, Reiko Nakanishi Itai, Nobuhiro Tsutsumi, Hiromi Nakanishi, Naoko K. Nishizawa

MOLECULAR PLANT　2　(5)　1059-1066　2009/09

DOI： 10.1093/mp/ssp051 　

ISSN： 1674-2052

eISSN： 1752-9867
OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints International-journal Peer-reviewed

Takahiro Aoyama, Takanori Kobayashi, Michiko Takahashi, Seiji Nagasaka, Kanako Usuda, Yusuke Kakei, Yasuhiro Ishimaru, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

PLANT MOLECULAR BIOLOGY　70　(6)　681-692　2009/08

DOI： 10.1007/s11103-009-9500-3 　

ISSN： 0167-4412

eISSN： 1573-5028
Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect International-journal Peer-reviewed

Kanako Usuda, Yasuaki Wada, Yasuhiro Ishimaru, Takanori Kobayashi, Michiko Takahashi, Hiromi Nakanishi, Yasuo Nagato, Satoshi Mori, Naoko K. Nishizawa

PLANT BIOTECHNOLOGY JOURNAL　7　(1)　87-95　2009/01

DOI： 10.1111/j.1467-7652.2008.00374.x 　

ISSN： 1467-7644
Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants Peer-reviewed

Yasuhiro Ishimaru, Motofumi Suzuki, Yuko Ogo, Michiko Takahashi, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

SOIL SCIENCE AND PLANT NUTRITION　54　(3)　417-423　2008/06

DOI： 10.1111/j.1747-0765.2008.00259.x 　

ISSN： 0038-0768
[Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil]. Peer-reviewed

Ishimaru Y, Nishizawa NK

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　53　(1)　65-71　2008/01
Publisher:
ISSN： 0039-9450
Overexpression of the OsZIP4 zinc transporter confers disarrangement of zinc distribution in rice plants International-journal Peer-reviewed

Yasuhiro Ishimaru, Hiroshi Masuda, Motofumi Suzuki, Khurram Bashir, Michiko Takahashi, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

JOURNAL OF EXPERIMENTAL BOTANY　58　(11)　2909-2915　2007/09

DOI： 10.1093/jxb/erm147 　

ISSN： 0022-0957

eISSN： 1460-2431
Cloning of a novel glutathione transporter, OsGTL1 from rice Peer-reviewed

Khurram Bashir, Yasuhiro Ishimaru, Takahiro Aoyama, Seiji Nagasaka, Michiko Takahashi, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

AMERICAN JOURNAL OF HEMATOLOGY　82　(6)　516-516　2007/06

ISSN： 0361-8609
The expression of iron homeostasis-related genes during rice germination International-journal Peer-reviewed

Tomoko Nozoye, Haruhiko Inoue, Michiko Takahashi, Yasuhiro Ishimaru, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

PLANT MOLECULAR BIOLOGY　64　(1-2)　35-47　2007/05

DOI： 10.1007/s11103-007-9132-4 　

ISSN： 0167-4412
Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil International-journal Peer-reviewed

Yasuhiro Ishimaru, Suyeon Kim, Takashi Tsukamoto, Hiroyuki Oki, Takanori Kobayashi, Satoshi Watanabe, Shinpei Matsuhashi, Michiko Takahashi, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　104　(18)　7373-7378　2007/05

DOI： 10.1073/pnas.0610555104 　

ISSN： 0027-8424
Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+ transporters OsIRT1 and OsIRT2 in rice Peer-reviewed

Hiromi Nakanishi, Ippei Ogawa, Yasuhiro Ishimaru, Satoshi Mori, Naoko K. Nishizawa

SOIL SCIENCE AND PLANT NUTRITION　52　(4)　464-469　2006/08

DOI： 10.1111/j.1747-0765.2006.00055.x 　

ISSN： 0038-0768
Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+. International-journal Peer-reviewed

Yasuhiro Ishimaru, Motofumi Suzuki, Takashi Tsukamoto, Kazumasa Suzuki, Mikio Nakazono, Takanori Kobayashi, Yasuaki Wada, Satoshi Watanabe, Shinpei Matsuhashi, Michiko Takahashi, Hiromi Nakanishi, Satoshi Mori, Naoko K Nishizawa

The Plant journal : for cell and molecular biology　45　(3)　335-46　2006/02

DOI： 10.1111/j.1365-313X.2005.02624.x 　

ISSN： 0960-7412

More details Close

Only graminaceous monocots possess the Strategy II iron (Fe)-uptake system in which Fe is absorbed by roots as an Fe3+-phytosiderophore. In spite of being a Strategy II plant, however, rice (Oryza sativa) contains the previously identified Fe2+ transporter OsIRT1. In this study, we isolated the OsIRT2 gene from rice, which is highly homologous to OsIRT1. Real-time PCR analysis revealed that OsIRT1 and OsIRT2 are expressed predominantly in roots, and these transporters are induced by low-Fe conditions. When expressed in yeast (Saccharomyces cerevisiae) cells, OsIRT2 cDNA reversed the growth defects of a yeast Fe-uptake mutant. This was similar to the effect of OsIRT1 cDNA. OsIRT1- and OsIRT2-green fluorescent protein fusion proteins localized to the plasma membrane when transiently expressed in onion (Allium cepa L.) epidermal cells. OsIRT1 promoter-GUS analysis revealed that OsIRT1 is expressed in the epidermis and exodermis of the elongating zone and in the inner layer of the cortex of the mature zone of Fe-deficient roots. OsIRT1 expression was also detected in the ccompanion cells. Analysis using the positron-emitting tracer imaging system showed that rice plants are able to take up both an Fe3+-phytosiderophore and Fe2+. This result indicates that, in addition to absorbing an Fe3+-phytosiderophore, rice possesses a novel Fe-uptake system that directly absorbs the Fe2+, a strategy that is advantageous for growth in submerged conditions.
OsZIP4, a novel zinc-regulated zinc transporter in rice International-journal Peer-reviewed

Y Ishimaru, M Suzuki, T Kobayashi, M Takahashi, H Nakanishi, S Mori, NK Nishizawa

JOURNAL OF EXPERIMENTAL BOTANY　56　(422)　3207-3214　2005/12

DOI： 10.1093/jxb/eri317 　

ISSN： 0022-0957

eISSN： 1460-2431

Show all ︎Show first 5

Misc. 61

Analysis of K and Na transporters in plants

内山剛志, 山梨太郎, 池田隼人, 菊永英寿, 石丸泰寛, 魚住信之

アイソトープ・放射線研究発表会(Web)　60th　2023

ISSN： 2436-4487
Na輸送体AtHKT1の生殖時における耐塩性機構の解明

内山剛志, 加藤恵, 池田隼人, 菊永英寿, 須田利美, 石丸泰寛, 魚住信之

日本土壌肥料学会講演要旨集(Web)　69　2023

ISSN： 2424-0575
空気を気体窒素肥料として応用する新規植物生育技術

山梨太郎, 山梨太郎, 武士将熙, 佐々木渉太, 高島圭介, 金子俊郎, 山上睦, TANUDJAJA Ellen, 石丸泰寛, 魚住信之

日本土壌肥料学会講演要旨集(Web)　68　2022

ISSN： 2424-0575
Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice (vol 109, pg 19166, 2012)

Satoru Ishikawa, Yasuhiro Ishimaru, Masato Igura, Masato Kuramata, Tadashi Abe, Takeshi Senoura, Yoshihiro Hase, Tomohito Arao, Naoko K. Nishizawa, Hiromi Nakanishi

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　115　(21)　E4950-E4951　2018/05

DOI： 10.1073/pnas.1806782115 　

ISSN： 0027-8424
Protein ligand-tethered synthetic calcium indicator for localization control and spatiotemporal calcium imaging in plant cells (vol 26, pg 9, 2016) International-journal

Yousuke Takaoka, Miyuki Shigenaga, Masaki Imai, Yuuki Nukadzuka, Yasuhiro Ishimaru, Kei Saito, Ryusuke Yokoyama, Kazuhiko Nishitani, Minoru Ueda

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　26　(12)　2974-2974　2016/06

DOI： 10.1016/j.bmcl.2016.04.050 　

ISSN： 0960-894X

eISSN： 1464-3405
ジャスモン酸共受容体サブタイブ選択的アゴニストの合理的設計とin vivo機能評価

高岡洋輔, 岩橋万奈, 斎藤大明, 江越脩祐, 加藤信樹, 石丸泰寛, 上田実

植物の生長調節　51　(Supplement)　2016

ISSN： 1346-5406
植物ホルモン受容体を標的としたサブタイプ選択的PPI誘起リガンドの開発

岩橋万奈, 江越脩祐, 齋藤大明, 石丸泰寛, 高岡洋輔, 上田実

天然有機化合物討論会講演要旨集(Web)　58th　2016

ISSN： 2433-1856
S09 Development of subtype selective agonist of Jasmonate Co-receptor

Takaoka Yousuke, Iwashita Toshiki, Iwahashi Mana, Hayashi Kengo, Suzuki Takeshi, Ishimaru Yasuhiro, Egoshi Shusuke, Ueda Minoru

50　31-31　2015/10/01
Publisher: The Japanese Society for Chemical Regulation of Plants
ISSN： 0919-1887
P075 GTR1 is involved in attenuation of excess JA responses by the JA-Ile translocation from wounded to undamaged leaves

Oikawa Takaya, Ishimaru Yasuhiro, Suzuki Takeshi, Takeishi Syohei, Matsuura Hideyuki, Takahashi Kousaku, Hamamoto Shin, Uozumi Nobuyuki, Shimizu Takafumi, Seo Mitsunori, Ohta Hiroyuki, Ueda Minoru

50　93-93　2015/10/01
Publisher: The Japanese Society for Chemical Regulation of Plants
ISSN： 0919-1887
P076 Jasmonic acid negatively regulates lateral root formation through the site-specific stabilization of Aux/IAAs in Arabidopsis thaliana

Suzuki Takeshi, Ishimaru Yasuhiro, Meesters Christian, Kombrink Erich, Chinen Takumi, Usui Takeo, Takahashi Kosaku, Matsuura Hideyuki, Hayashi Ken-ichiro, Fukaki Hidehiro, Ueda Minoru

50　94-94　2015/10/01
Publisher: The Japanese Society for Chemical Regulation of Plants
ISSN： 0919-1887
4-2-1 イネ鉄欠乏応答におけるユビキチンリガーゼHRZとジャスモン酸経路の関与(4-2 植物の微量栄養素,2015年度京都大会)

小林高範, 板井玲子, 瀬野浦武志, 及川貴也, 石丸泰寛, 上田実, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(61)　65-65　2015/09/09
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
植物毒素コロナチンの誘導体化による植物ホルモン受容体サブタイプ選択的アゴニスト開発と未知標的探索

高岡洋輔, 岩下利基, 岩橋万奈, 鈴木健史, 林謙吾, 石丸泰寛, 江越脩祐, 上田実

天然有機化合物討論会講演要旨集(Web)　57th　2015

ISSN： 2433-1856
CYP94ファミリー遺伝子の発現上昇はイネの塩処理条件下での生存能を高める

黒谷賢一, 林憲志, 畑中彩希, 戸田陽介, 小川大輔, 市川裕章, 石丸泰寛, 田下諒, 鈴木健史, 上田実, 服部束穂, 武田真

日本植物生理学会年会要旨集　56th　2015
84. Comprehensive analysis of Samanea K^+ channels involved in nyctinasty

Oikawa Takaya, Ishimaru Yasuhiro, Hamamoto Shin, Uozumi Nobuyuki, Ueda Minoru

48　99-99　2013/10/04
Publisher: The Japanese Society for Chemical Regulation of Plants
ISSN： 0919-1887
41. Synthesis of alkyne-containing coronatines : towards application to in vivo Raman imaging

Egoshi Syusuke, Tashita Ryo, Yamakoshi Hiroyuki, Dodo Kosuke, Ishimaru Yasuhiro, Genji Takahisa, Sodeoka Mikiko, Ueda Minoru

48　56-56　2013/10/04
Publisher: The Japanese Society for Chemical Regulation of Plants
ISSN： 0919-1887
4-2-12 ムギネ酸類生合成系遺伝子とフェリチン遺伝子の同時導入によるイネの鉄栄養の強化(4-2 植物の微量栄養素)

増田寛志, 小林高範, 石丸泰寛, 高橋美智子, Aung May Sann, 中西啓仁, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(59)　70-70　2013/09/11
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
P10-3 イネの根から地上部へのZn、Cd輸送におけるHMA2トランスポーターの役割(10.植物の微量栄養素,2012年度鳥取大会)

高橋竜一, 石丸泰寛, Shimo Hugo, 小郷裕子, 瀬野浦武志, 西澤直子, 中西啓仁

日本土壌肥料学会講演要旨集　(58)　67-67　2012/09/04
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
P11-3 イオンビーム照射でカドミウム極低コシヒカリの作出に成功(11.植物の有害元素,2012年度鳥取大会)

石川覚, 安部匡, 倉俣正人, 井倉将人, 石丸泰寛, 長谷純宏, 荒尾知人, 西澤直子, 中西啓仁

日本土壌肥料学会講演要旨集　(58)　84-84　2012/09/04
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
P10-5 鉄欠乏誘導性転写因子OsIRO2の高発現と改変型三価鉄還元酵素遺伝子refre1/372を組み合わせた石灰質士壌耐性イネの作出第二報(10.植物の微量栄養素,2012年度鳥取大会)

下地絵理香, 増田寛志, 濱田達朗, 小林高範, 石丸泰寛, 小郷裕子, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(58)　68-68　2012/09/04
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
低カドミウム形質を持つコシヒカリ変異体の特徴(2)分子・遺伝的解析

中西啓仁, 石丸泰寛, シモウーゴ, 瀬野浦武志, 伊倉将人, 安部匡, 倉俣正人, 石川覚, 西澤直子, 西澤直子

日本植物生理学会年会要旨集　53rd　2012
低カドミウム形質を持つコシヒカリ変異体の特徴(1)農業形質・生理的解析

石川覚, 井倉将人, 安部匡, 倉俣正人, 石丸泰寛, 中西啓仁, 西澤直子, 西澤直子

日本植物生理学会年会要旨集　53rd　2012
OsYSL16はイネ三価鉄体内移行に関わる

筧雄介, 石丸泰寛, 小林高範, 山川隆, 中西啓仁, 西澤直子, 西澤直子

日本植物生理学会年会要旨集　53rd　2012
11-5 イネのカドミウム吸収に関わる鉄トランスポーターの解析第3報(11.植物の有害元素)

高橋竜一, 石丸泰寛, 瀬野浦武志, Shimo Hugo, 石川覚, 荒尾知人, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(57)　80-80　2011/08/08
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
11-22 ナスおよび低カドミウム蓄積性ナス属トルバムにおけるカドミウム処理による遺伝子発現変化(11.植物の有害元素)

瀬野浦武志, 石丸泰寛, 高橋竜一, 福岡浩之, 荒尾知人, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(57)　86-86　2011/08/08
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
11-18 遺伝子組換えによるカドミウムファイトレメディエーション用イネの創製(11.植物の有害元素)

石丸泰寛, Shimo Hugo, 高橋竜一, 瀬野浦武志, 杉本和彦, 小野和子, 矢野昌裕, An Gynheung, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(57)　84-84　2011/08/08
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
10-2 鉄欠乏誘導性転写因子の発現強化と鉄還元能力の強化による石灰質アルカリ土壌耐性イネの作出(10.植物の微量栄養素)

下地絵理香, 増田寛志, 濱田達朗, 石丸泰寛, 小郷裕子, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(57)　69-69　2011/08/08
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
イネのプロトカテク酸放出トランスポーター

石丸泰寛, 筧雄介, 佐藤豊, 佐藤祐樹, 魚住信之, 中西啓仁, 西澤直子, 西澤直子

日本植物生理学会年会要旨集　52nd　2011
III-6 Fe栄養の分子生理(III 植物の必須元素の栄養生理,シンポジウム,2010年度北海道大会)

中西啓仁, 石丸泰寛, Bashir Khurram, 西澤直子

日本土壌肥料学会講演要旨集　(56)　216-216　2010/09/07
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
P11-9 イネのカドミウム吸収に関わる鉄トランスポーターの解析第2報(ポスター紹介,11.植物の有害元素,2010年度北海道大会)

高橋竜一, 石丸泰寛, 瀬野浦武志, Shimo Hugo, 石川覚, 荒尾知人, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(56)　87-87　2010/09/07
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
11-1 シナマンネングサ(Sedum alfredii)におけるカドミウム超蓄積機構の解析(11.植物の有害元素,2010年度北海道大会)

Zhang Min, 瀬野浦武志, Yang Xiao-E, 石丸泰寛, 高橋竜一, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(56)　75-75　2010/09/07
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
10-12 鉄の体内輸送と蓄積能を同時に強化した高鉄米の隔離圃場検定(10.植物の微量栄養素,2010年度北海道大会)

増田寛志, 石丸泰寛, メイサンアウン, 小林高範, 高橋美智子, 樋口恭子, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(56)　71-71　2010/09/07
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
10-3 イネのプロトカテク酸放出トランスポーター(10.植物の微量栄養素,2010年度北海道大会)

石丸泰寛, 筧雄介, バシルクーラム, 佐藤祐樹, 魚住信之, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　56　(56)　68-68　2010/09/07
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
奨-1 イネの鉄・亜鉛トランスポーターに関する研究(日本土壌肥料学会奨励賞記念講演,2009年度京都大会)

石丸泰寛

日本土壌肥料学会講演要旨集　(55)　237-237　2009/09/15
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
11-22 イネのカドミウム吸収に関わる鉄トランスポーターの解析(11.植物の有害元素,2009年度京都大会)

高橋竜一, 石丸泰寛, 瀬野浦武志, Shimo Hugo, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(55)　96-96　2009/09/15
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
P11-11 ナス属由来ZIPトランスポーターの機能解析(ポスター紹介,11.植物の有害元素,2009年度京都大会)

瀬野浦武志, 石丸泰寛, 高橋竜一, Shimo Hugo, 福岡浩之, 荒尾知人, 石川覚, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(55)　101-101　2009/09/15
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
P11-12 Low cadmium (LCD), a novel gene related to cadmium tolerance and accumulation in rice seeds

Shimo Hugo, Ishimaru Yasuhiro, Takahashi Ryuichi, Senoura Takeshi, An Gynheung, Nakananishi Hiromi, Nishizawa Naoko

Abstracts of the meeting, the Society of the Science of Soil and Manure　(55)　102-102　2009/09/15
Publisher: Japanese Society of Soil Science and Plant Nutrition
ISSN： 0288-5840
P10-6 イネの根における鉄欠乏初期の遺伝子発現解析(ポスター紹介,10.植物の微量栄養素,2009年度京都大会)

板井玲子, 小郷裕子, 石丸泰寛, 小林高範, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(55)　88-88　2009/09/15
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
10-8 鉄の体内輸送と蓄積能を同時に強化した高鉄米の作出(10.植物の微量栄養素,2009年度京都大会)

増田寛志, 石丸泰寛, 小林高範, 高橋美智子, 大杉立, 青木直大, 吉原利一, 高岩文雄, 樋口恭子, 中西啓仁, 西澤直子

日本土壌肥料学会講演要旨集　(55)　82-82　2009/09/15
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
9-28 植物由来の血圧降下物質ニコチアナミン高蓄積米の創製(9.植物の無機栄養,2007年度東京大会)

臼田華奈子, 和田泰明, 高橋美智子, 石丸泰寛, 中西啓仁, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(53)　72-72　2007/08/22
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
9-12 カドミウムに対するイネ遺伝子発現応答(9.植物の無機栄養,2007年度東京大会)

小川一平, 中西啓仁, 小林高範, 石丸泰寛, 高橋美智子, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(53)　66-66　2007/08/22
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
イネ種子における金属分布と金属輸送

高橋美智子, 北島信行, 保倉明子, 寺田靖子, 長坂征治, 井上晴彦, 石丸泰寛, 中西啓仁, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　53　(53)　71-71　2007/08/22
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
イネ種子胚における金属分布と胚への金属輸送

高橋美智子, 北島信行, 北島信行, 保倉明子, 寺田靖子, 鈴木基史, 井上晴彦, 石丸泰寛, 中西啓仁, 森敏, 西澤直子, 西澤直子

日本植物生理学会年会要旨集　48th　2007
生殖成長および種子成熟期における金属動態の解明

高橋美智子, 北島信行, 保倉明子, 寺田靖子, 井上晴彦, 石丸泰寛, 中西啓仁, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　53　2007

ISSN： 0288-5840
Overexpression of the OsZIP4 zinc transporter induces disarrangement of zinc distribution in rice.

Yasuhiro Ishimaru, Hiroshi Masuda, Motofumi Suzuki, Michiko Takahashi, Hiromi Nakanishi, Satishi Mori, Naoko Nishizawa

PLANT AND CELL PHYSIOLOGY　48　S89-S89　2007

ISSN： 0032-0781
How are metal nutrients transported to the embryo in rice plant?

TAKAHASHI Michiko, INOUE Haruhiko, ISHIMARU Yasuhiro, NAKANISHI Hiromi, MORI Satoshi, NISHIZAWA Naoko

119　173-173　2006/12/01

ISSN： 0918-9440
P9-13 イネにおける金属元素の種子への輸送(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

高橋美智子, 井上晴彦, 石丸泰寛, 中西啓仁, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(52)　78-78　2006/09/05
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
P9-12 イネのカドミウム吸収における鉄の影響(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

小川一平, 中西啓仁, 石丸泰寛, 高橋美智子, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(52)　77-77　2006/09/05
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
9-22 Characterization of Glutathione transporter in rice

Bashir Khurram, Ishimaru Yasuhiro, Inoue Haruhiko, Nagasaka Seiji, Takahashi Michiko, Nakanishi Hiromi, Mori Satoshi, Nishizawa Naoko

Abstracts of the meeting, the Society of the Science of Soil and Manure　(52)　58-58　2006/09/05
Publisher: Japanese Society of Soil Science and Plant Nutrition
ISSN： 0288-5840
9 鉄吸収機構を強化した高鉄含有米の創製 (第1報)(関東支部講演会, 2005年度各支部会講演要旨)

増田寛志, 石丸泰寛, 小林高範, 高橋美智子, 中西啓仁, 吉原利一, 高岩文雄, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(52)　258-258　2006/09/05
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
Expression analysis of rice genes involved in iron acquisition during seed germination

Tomoko Nozoye, Haruhiko Inoue, Yasuhiro Ishimaru, Michiko Takahashi, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

13th International Symposium on Iron Nutrition and Interactions in Plants　2006/06
9-40 鉄の吸収機構を強化した高鉄含有米の創製(第二報)(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

増田寛志, 小林高範, 石丸泰寛, 高橋美智子, 中西啓仁, 吉原利一, 高岩文雄, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　52　64-64　2006
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
Directed evolution of yeast ferric chelate reductase confers enhanced tolerance of rice to iron deficiency in a calcareous soil

Y Ishimaru, S Kim, T Tsukamoto, M Takahashi, H Nakanishi, S Mori, N Nishizawa

PLANT AND CELL PHYSIOLOGY　47　S157-S157　2006

ISSN： 0032-0781
The role of nicotianamine and mugineic acid in metal transport at reproductive stage

M Takahashi, H Inoue, Y Ishimaru, H Nakanishi, S Mori, N Nishizawa

PLANT AND CELL PHYSIOLOGY　47　S230-S230　2006

ISSN： 0032-0781
Iron-fortification of rice seeds by expressing ferritin genes along with enhancing iron absorption and translocation

H Masuda, T Kobayashi, Y Ishimaru, M Takahashi, H Nakanishi, T Yoshihara, H Takaiwa, S Mori, N Nishizawa

PLANT AND CELL PHYSIOLOGY　47　S203-S203　2006

ISSN： 0032-0781
Fe-deficiency enhanced Cd uptake and translocation by Fe2+-transporters, OsIRT1 and OsIRT2, in rice

Ogawa, I, H Nakanishi, Y Ishimaru, M Takahashi, S Mori, NK Nishizawa

PLANT AND CELL PHYSIOLOGY　47　S231-S231　2006

ISSN： 0032-0781
9-18 イネにおける3価鉄還元酵素遺伝子の解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

石丸泰寛, 鈴木基史, 塚本崇志, 金透蓮, 高橋美智子, 中西啓仁, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(51)　63-63　2005/09/06
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
Microarray analysis on genes induced by Zn deficiency in rice

M Suzuki, Y Ishimaru, H Inoue, M Takahashi, H Nakanishi, S Mori, N Nishizawa

PLANT AND CELL PHYSIOLOGY　46　S247-S247　2005

ISSN： 0032-0781
血圧降下作用を持った機能性食品の開発にむけて : ニコチアナミン高蓄積米の創製(9. 植物の無機栄養, 2004年度大会講演要旨集)

和田泰明, 石丸泰寛, 中西啓仁, 高橋美智子, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(50)　81-81　2004/09/14
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
イネのOsIRT1ホモログ (OsIRT) 遺伝子群の解析(9. 植物の無機栄養, 2004年度大会講演要旨集)

石丸泰寛, 鈴木基史, 高橋美智子, 中西啓仁, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(50)　77-77　2004/09/14
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
9-20 イネの二価鉄イオントランスポーター(OsIRT1,OsIRT2)の解析(9.植物の無機栄養)

石丸泰寛, 中西啓仁, 高橋美智子, 鈴木基史, 和田泰明, 森敏, 西澤直子

日本土壌肥料学会講演要旨集　(49)　71-71　2003/08/20
Publisher: 一般社団法人日本土壌肥料学会
ISSN： 0288-5840
OsIRT1 and OsIRT2, rice transporters essential for iron uptake from the soil and for plant growth

Y Ishimaru, H Nakanishi, M Takahashi, H Yamaguchi, B Naimatullah, S Mori, N Nishizawa

PLANT AND CELL PHYSIOLOGY　44　S96-S96　2003

ISSN： 0032-0781

Show all ︎Show first 5

Research Projects 10

超硫黄脂質の脱着による気孔の可逆的運動の解明

石丸泰寛

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2025/04/01 - 2028/03/31
Management of international relation and facility for promotion of research on sulfur biology

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Transformative Research Areas (A)

Category: Grant-in-Aid for Transformative Research Areas (A)

Institution: Tohoku University

2021/09/10 - 2026/03/31
光合成における超硫黄分子の貢献

石丸泰寛

Offer Organization: 日本学術振興会

System: 科学研究費助成事業学術変革領域研究(A)

Category: 学術変革領域研究(A)

Institution: 東北大学

2021/09/10 - 2026/03/31
超硫黄修飾がもたらす気孔閉鎖の多段的認識機構の解明

石丸泰寛

Offer Organization: 日本学術振興会

System: 科学研究費助成事業挑戦的研究(萌芽)

Category: 挑戦的研究(萌芽)

Institution: 東北大学

2022/06/30 - 2025/03/31
Study on the molecular mechanism of Ca signaling mediated by plant K channels

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Institution: Tohoku University

2020/10/27 - 2024/03/31
Elucidation of hierarchical functional control of plant ion transporters driven by intracellular regulatory factors

Uozumi Nobuyuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2019/04/01 - 2022/03/31

More details Close

I Living cells need to adapt to various changes in the environment, and have a mechanism to maintain homeostasis in response to drought, dehydration, salt damage, etc. The plant ion transporter functions as a molecular mechanism, and performs nutrient absorption, osmoregulation, membrane potential formation, and activation and inactivation of intracellular enzymes. Ion transport system located in biological membrane play important roles in maintaining homeostasis, proliferation and differentiation in response to environmental changes. In this study, we have elucidated the functions and roles of ion transport system, which have not been well characterized, and their control mechanism. We have identified ion transport system to adapt to environmental changes and obtained knowledge on the control mechanism of membrane ion transport.
Analysis of ion transport of nyctinasty in leguminous plants

Ishimaru Yasuhiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2019/04/01 - 2022/03/31

More details Close

We specifically analyzed Flexor motility cells of the leguminous Samanea Saman(Fabaceae), a model plant for nyctinasty. To understand the regulation mechanism of nyctinasty via ion channels, we identified Ca2+ channels as the starting point of nyctinasty and examined why nyctinasty is necessary by capturing the subsequent signal transduction. Using transformed plants expressing a fluorescent Ca2+ sensor, we failed to capture the moment of Ca2+ influx by Ca2+ channels in Flexor motility cells and its subsequent propagation. We have found an ion channel inhibitor for Arabidopsis thaliana, evaluated the performance of the compound, and succeeded in applying the compound to Samanea Saman.
気孔閉口運動を制御するカルシウムイオン輸送体の解析

石丸泰寛

Offer Organization: 日本学術振興会

System: 科学研究費助成事業国際共同研究加速基金(国際共同研究強化(A))

Category: 国際共同研究加速基金(国際共同研究強化(A))

Institution: 東北大学

2021 - 2021
Development of selective antagonist of COI1-JAZ receptor

Yasuhiro Ishimaru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

Category: Grant-in-Aid for Young Scientists (B)

Institution: Tohoku University

2015/04/01 - 2017/03/31

More details Close

In this study, JAZ subtype-selective agonists, which selectively activate useful functions among diverse physiological roles by jasmonic acids signaling, are developed based on coronatine which is a mimic of jasmonoyl-L-isoleucine with potent activities. A lead compound used for JAZ selective agonist development was found using an evaluation system of pull-down assay system COI1-JAZ interaction using JAZ fragment peptide which is a binding domain with COI1 of JAZ. Based on the knowledge, we designed several compounds that are thought to be more selective improvement by in silico docking study. By a similar pull-down assay, two subtype-selective agonists that bind to some of JAZ were found among them.
Circadian rhythm changes and biological phenomenon by endogenous biological activator Competitive

ISHIMARU Yasuhiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up

Category: Grant-in-Aid for Research Activity Start-up

Institution: Tohoku University

2011 - 2012

More details Close

The molecular mechanism is hardly revealed in Samanea saman which shows nyctinastic movement. In this research, the measurement method of the smaller amount of the compounds for the nyctinasty was established in Samanea saman, and the concentration change according to nyctinasty was measured. In addition, the full length complete cDNAs, which would be related to nyctinasty, were isolated. Their gene expression patterns were also analyzed by qPCR.

Show all Show first 5