Details of the Researcher

PHOTO

Shinya Nakamura
Section
Graduate School of Life Sciences
Job title
Specially Appointed Associate Professor(Research)
Degree

  • 生命科学博士 (Tohoku University)

Research History 5

  • 2024/04 - Present
    Tohoku University　Graduate School of Life Sciences　Project Associate Professor

  • 2023/04 - 2024/03
    National Institute for Physiological Sciences　Division of Neural Dynamics　Project Assistant Professor

  • 2014/01 - 2023/03
    Tohoku University　Graduate School of Life Sciences　Project Assistant Professor

  • 2010/04 - 2013/12
    University of Colorado Boulder　Institute for Behavioral Genetics　Research Associate

  • 2009/04 - 2010/03
    Tohoku University　Graduate School of Life Sciences　Project Assistant Professor

Education 3

  • Tohoku University　Graduate School of Life Sciences

    2006/04 - 2009/03

  • Tohoku University　Graduate School of Life Sciences

    2004/04 - 2006/03

  • Kyushu University　School of Sciences　Department of Biology

    2000/04 - 2004/03

Professional Memberships 4

  • 日本心理学会

  • 日本生理学会

  • The Japan Neuroscience Society

  • Society for Neuroscience

Research Interests 6

  • self-body recognition

  • working memory

  • motivation

  • emotion

  • monkey

  • transcranial magnetic stimulation

Research Areas 4

  • Humanities & social sciences / Experimental psychology /

  • Life sciences / Basic brain sciences /

  • Life sciences / Nervous system function /

  • Life sciences / Neuroscience - general /

Awards 1

  1. 公益社団法人日本心理学会学術大会特別優秀発表賞

    2024/01　日本心理学会　サルは訓練によりVirtual Reality上の身体像を自己身体として認識する

Papers 16

  1. Anterior cingulate cortex projections to the amygdala in primates: topographic and layer-specific organization underlying emotion and mood regulation

    Kei Kimura, Yuki Soga, Rintaro Yoshino, Andi Zheng, Satoshi Nonomura, Gaoge Yan, Soshi Tanabe, Shinya Nakamura, Shinya Ohara, Ken-ichi Inoue, Masahiko Takada, Ken-Ichiro Tsutsui

    Journal of Neuroscience　e0671262026-e0671262026　2026/08/14

    Publisher: Society for Neuroscience

    DOI： 10.1523/jneurosci.0671-26.2026 　

    ISSN： 0270-6474

    eISSN： 1529-2401

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    Emotion and mood regulation critically depends on interactions between the anterior cingulate cortex (ACC) and the amygdala. However, the detailed architecture of ACC projections to their major targets, the basal (BA) and accessory (AcBA) basal nuclei of the amygdala, remains unclear. To address this issue, a combined retrograde and anterograde tracing with viral vectors were performed in macaques of either sex to map the projection patterns from the pregenual (pgACC), subgenual (sgACC), and dorsal (dACC) subareas. Data revealed that ACC neurons projecting to the BA arose predominantly from the superficial layers (II/III) of all subareas and the deep layers (V/VI) of the sgACC, whereas ACC neurons projecting to the AcBA originated mainly in the deep layers of the sgACC and dACC. The present study defines the topographic and layer-specific organization of ACC–amygdala connectivity in primates and subserves to provide an anatomical basis for future causal and translational approaches, such as targeted interventions against ACC-related mood disorders. Significance Statement Emotion and mood regulation critically depends on interactions between the anterior cingulate cortex (ACC) and the amygdala, and their dysfunctions have been implicated in mood disorders such as depression. However, the detailed organization of primate ACC–amygdala circuitry remains to be fully understood. Notably, the existence of projections from the pregenual ACC (pgACC) to the basal nucleus of the amygdala (BA) has been controversial. Using viral vector-based neural tracings in macaques, we have identified a distinct pgACC–BA pathway and shown that this pathway arises predominantly from the superficial layers of the cortex. Such unexpected laminar origin, atypical for cortico–subcortical projections, reveals a previously unrecognized circuit architecture and challenges prevailing models of cortico–amygdala linkage involved in mood disorders.

  2. Depression induced by low-frequency repetitive transcranial magnetic stimulation to ventral medial frontal cortex in monkeys Peer-reviewed

    Shinya Nakamura, Yodai Kishimoto, Masaki Sekino, Motoaki Nakamura, Ken-Ichiro Tsutsui

    Experimental Neurology　357　114168-114168　2022/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.expneurol.2022.114168 　

    ISSN： 0014-4886

  3. Laminar Organization of the Entorhinal Cortex in Macaque Monkeys Based on Cell-Type-Specific Markers and Connectivity Peer-reviewed

    Shinya Ohara, Rintaro Yoshino, Kei Kimura, Taichi Kawamura, Soshi Tanabe, Andi Zheng, Shinya Nakamura, Ken-ichi Inoue, Masahiko Takada, Ken-Ichiro Tsutsui, Menno P. Witter

    Frontiers in Neural Circuits　15　2021/12/07

    Publisher: Frontiers Media SA

    DOI： 10.3389/fncir.2021.790116 　

    eISSN： 1662-5110

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    The entorhinal cortex (EC) is a major gateway between the hippocampus and telencephalic structures, and plays a critical role in memory and navigation. Through the use of various molecular markers and genetic tools, neuron types constituting EC are well studied in rodents, and their layer-dependent distributions, connections, and functions have also been characterized. In primates, however, such cell-type-specific understandings are lagging. To bridge the gap between rodents and primates, here we provide the first cell-type-based global map of EC in macaque monkeys. The laminar organization of the monkey EC was systematically examined and compared with that of the rodent EC by using immunohistochemistry for molecular markers which have been well characterized in the rodent EC: reelin, calbindin, and Purkinje cell protein 4 (PCP4). We further employed retrograde neuron labeling from the nucleus accumbens and amygdala to identify the EC output layer. This cell-type-based approach enabled us to apply the latest laminar definition of rodent EC to monkeys. Based on the similarity of the laminar organization, the monkey EC can be divided into two subdivisions: rostral and caudal EC. These subdivisions likely correspond to the lateral and medial EC in rodents, respectively. In addition, we found an overall absence of a clear laminar arrangement of layer V neurons in the rostral EC, unlike rodents. The cell-type-based architectural map provided in this study will accelerate the application of genetic tools in monkeys for better understanding of the role of EC in memory and navigation.

  4. 霊長類のうつ病モデル

    中村晋也, 筒井健一郎

    臨床神経科学　39　(8)　1020-1023　2021/08

  5. Changes in beta and high-gamma power in resting-state electrocorticogram induced by repetitive transcranial magnetic stimulation of primary motor cortex in unanesthetized macaque monkeys International-journal Peer-reviewed

    Yasutaka Honda, Shinya Nakamura, Kentaro Ogawa, Rintaro Yoshino, Philippe N. Tobler, Yukio Nishimura, Ken-Ichiro Tsutsui

    Neuroscience Research　171　41-48　2021/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.neures.2021.02.002 　

    ISSN： 0168-0102

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    Repetitive transcranial magnetic stimulation (rTMS) is now widely used as a means of neuromodulation, but the details of the mechanisms by which rTMS works remain unclarified. As a step forward to unveiling the neural phenomena occurring underneath the TMS coil, we conducted an electrophysiological study using awake and unanesthetized monkeys with subdural electrocorticogram (ECoG) electrodes implanted over the primary motor cortex (MI). We evaluated the effects of low-frequency (1 Hz) and high-frequency (10 Hz) rTMS on the resting-state ECoG signals in the stimulated MI, as well as the motor evoked potentials (MEPs) in the contralateral hand. Following the 1-Hz rTMS application, the ECoG beta band power and the MEP amplitude were significantly decreased. Following the 10-Hz rTMS application, the ECoG high-gamma power and the MEP amplitude significantly increased. Given that beta and high-gamma activities in the ECoG reflect the synchronous firing and the firing frequency of cell assemblies, respectively, in local neural circuits, these results suggest that low-frequency rTMS inhibits neural activity by desynchronizing the firing activity of local circuits, whereas high-frequency rTMS facilitates neural activity by increasing the firing rate of cell assemblies in the local circuits.

  6. 経頭蓋磁気刺激による大脳皮質機能研究の新たな展開

    筒井健一郎, 中村晋也

    Clinical Neuroscience　38　(2)　193-195　2020/02

  7. rTMSで探る内側前頭皮質の気分・情動制御メカニズム

    中村晋也, 筒井健一郎

    臨床精神医学　47　(8)　883-887　2018/08

  8. The Role of Monkey Medial Frontal Cortex in the Regulation of Affect and Mood Examined by Repetitive Trans-cranial Magnetic Stimulation (rTMS)

    Tsutsui Ken-Ichiro, Nakamura Shinya

    Japanese Journal of Psychosomatic Medicine　57　(9)　916-921　2017

    Publisher: Japanese Society of Psychosomatic Medicine

    DOI： 10.15064/jjpm.57.9_916 　

    ISSN： 0385-0307

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    <p>TMS is a powerful tool for manipulating brain activity. However, how exactly it works still remains poorly understood. Here, we recorded electrocorticograms (ECoG) before and after repetitive trans-cranial magnetic stimulation (rTMS). We also recorded motor evoked potentials (MEP) evoked by single-pulse TMS before and after rTMS. After low-frequency (1 Hz) rTMS, MEP amplitude was suppressed and we observed a decrease in beta-band power of the ECoG ; conversely, after high-frequency (10 Hz) rTMS, MEP amplitude was enhanced and we observed an increase of gamma-band power in the ECoG. These results indicate that low-frequency and high-frequency rTMS induce systematic changes in the background cortical neural activity. We next examined the function of the medial frontal cortex (MFC) by applying low-frequency (1 Hz) rTMS inhibiting local neural activity. We used a double cone coil to stimulate the ventral part of the MFC including subgenual anterior cingulate cortex (sgACC), and figure-of-eight coil to stimulate only the dorsal part of MFC as control. Stimulation had a clear and profound impact on behavior only when a double-cone coil was used. Specifically the monkey exhibited changes in physiological and behavioral measures that indicated sustained depression of mood and emotion, such as elevated blood cortisol levels, decreased within-cage spontaneous activity, and social withdrawal (unwillingness to interact with research staff). These results reveal a critical involvement of the ventral part of MFC in the regulation of mood and motivation.</p>

  9. Comparative Overview of Visuospatial Working Memory in Monkeys and Rats

    Ken-Ichiro Tsutsui, Kei Oyama, Shinya Nakamura, Toshio Iijima

    Frontiers in Systems Neuroscience　10　2016/12/16

    Publisher: Frontiers Media SA

    DOI： 10.3389/fnsys.2016.00099 　

    eISSN： 1662-5137

  10. Control over stress accelerates extinction of drug seeking via prefrontal cortical activation Peer-reviewed

    Michael V. Baratta, Matthew B. Pomrenze, Shinya Nakamura, Samuel D. Dolzani, Donald C. Cooper

    Neurobiology of Stress　2　20-27　2015

    Publisher: Elsevier BV

    DOI： 10.1016/j.ynstr.2015.03.002 　

    ISSN： 2352-2895

  11. A novel variable delay Go/No-Go task to study attention, motivation and working memory in the head-fixed rodent Peer-reviewed

    Samuel D Dolzani, Shinya Nakamura, Donald C Cooper

    F1000Research　2　125-125　2014/03/19

    Publisher: F1000 Research Ltd

    DOI： 10.12688/f1000research.2-125.v2 　

    eISSN： 2046-1402

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    <ns4:p>In order to parse the causal elements underlying complex behaviors and decision-making processes, appropriate behavioral methods must be developed and used in concurrence with molecular, pharmacological, and electrophysiological approaches. Presented is a protocol for a novel Go/No-Go behavioral paradigm to study the brain attention and motivation/reward circuitry in awake, head-restrained rodents. This experimental setup allows: (1) Pharmacological and viral manipulation of various brain regions via targeted guide cannula; (2) Optogenetic cell-type specific activation and silencing with simultaneous electrophysiological recording and; (3) Repeated electrophysiological single and multiple unit recordings during ongoing behavior. The task consists of three components. The subject first makes an observing response by initiating a trial by lever pressing in response to distinctive Go or No-Go tones.  Then, after a variable delay period, the subject is presented with a challenge period cued by white noise during which they must respond with a lever press for the Go condition or withhold from lever pressing for the duration of the cue in the No-Go condition. After correctly responding during the challenge period (Challenge) and a brief delay, a final reward tone of the same frequency as the initiation tone is presented and sucrose reward delivery is available and contingent upon lever pressing. Here, we provide a novel procedure and validating data set that allows researchers to study and manipulate components of behavior such as attention, motivation, impulsivity, and reward-related working memory during an ongoing operant behavioral task while limiting interference from non task-related behaviors.</ns4:p>

  12. A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons <em>In vivo</em> Peer-reviewed

    Shinya Nakamura, Michael V. Baratta, Donald C. Cooper

    Journal of Visualized Experiments　(79)　2013/09/02

    Publisher: MyJove Corporation

    DOI： 10.3791/50291 　

    eISSN： 1940-087X

  13. Cocaine self-administration in mice with forebrain knock-down of trpc5 ion channels Peer-reviewed

    Matthew B Pomrenze, Michael V Baratta, Kristin C Rasmus, Brian A Cadle, Shinya Nakamura, Lutz Birnbaumer, Donald C Cooper

    F1000Research　2　53-53　2013/02/15

    Publisher: F1000 Research Ltd

    DOI： 10.12688/f1000research.2-53.v1 　

    eISSN： 2046-1402

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    <ns4:p>Canonical transient receptor potential (TRPC) channels are a family of non-selective cation channels that play a crucial role in modulating neuronal excitability due to their involvement in intracellular Ca2+ regulation and dendritic growth. TRPC5 channels a) are one of the two most prevalent TRPC channels in the adult rodent brain; b) are densely expressed in deep layer pyramidal neurons of the prefrontal cortex (PFC); and c) modulate neuronal persistent activity necessary for working memory and attention. In order to evaluate the causal role of TRPC5 in motivation/reward-related behaviors, conditional forebrain TRPC5 knock-down (trpc5-KD) mice were generated and trained to nose-poke for intravenous cocaine. Here we present a data set containing the first 6 days of saline or cocaine self-administration in wild type (WT) and trpc5-KD mice. In addition, we also present a data set showing the dose-response to cocaine after both groups had achieved similar levels of cocaine self-administration. Compared to WT mice, trpc5-KD mice exhibited an apparent increase in self-administration on the first day of cocaine testing without prior operant training. There were no apparent differences between WT and trpc5-KD mice for saline responding on the first day of training. Both groups showed similar dose-response sensitivity to cocaine after several days of achieving similar levels of cocaine intake.</ns4:p>

  14. High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo Peer-reviewed

    Shinya Nakamura, Michael V Baratta, Matthew B Pomrenze, Samuel D Dolzani, Donald C Cooper

    F1000Research　1　7-7　2012/07/30

    Publisher: F1000 Research Ltd

    DOI： 10.12688/f1000research.1-7.v1 　

    eISSN： 2046-1402

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    <ns4:p>Precise spatial and temporal manipulation of neural activity in specific genetically defined cell populations is now possible with the advent of optogenetics. The emerging field of optogenetics consists of a set of naturally-occurring and engineered light-sensitive membrane proteins that are able to activate (e.g. channelrhodopsin-2, ChR2) or silence (e.g. halorhodopsin, NpHR) neural activity. Here we demonstrate the technique and the feasibility of using novel adeno-associated viral (AAV) tools to activate (AAV-CaMKllα-ChR2-eYFP) or silence (AAV-CaMKllα-eNpHR3.0-eYFP) neural activity of rat prefrontal cortical prelimbic (PL) pyramidal neurons <ns4:italic>in vivo</ns4:italic>. <ns4:italic>In vivo</ns4:italic> single unit extracellular recording of ChR2-transduced pyramidal neurons showed that delivery of brief (10 ms) blue (473 nm) light-pulse trains up to 20 Hz via a custom fiber optic-coupled recording electrode (optrode) induced spiking with high fidelity at 20 Hz for the duration of recording (up to two hours in some cases). To silence spontaneously active neurons, we transduced them with the NpHR construct and administered continuous green (532 nm) light to completely inhibit action potential activity for up to 10 seconds with 100% fidelity in most cases. These versatile photosensitive tools, combined with optrode recording methods, provide experimental control over activity of genetically defined neurons and can be used to investigate the functional relationship between neural activity and complex cognitive behavior.</ns4:p>

  15. Difference in the functional significance between the lemniscal and paralemniscal pathways in the perception of direction of single-whisker stimulation examined by muscimol microinjection Peer-reviewed

    Shinya Nakamura, Takaaki Narumi, Ken-Ichiro Tsutsui, Toshio Iijima

    Neuroscience Research　64　(3)　323-329　2009/07

    DOI： 10.1016/j.neures.2009.04.005 　

    ISSN： 0168-0102

  16. Impairment of the discrimination of the direction of single-whisker stimulation induced by the lemniscal pathway lesion Peer-reviewed

    Takaaki Narumi, Shinya Nakamura, Ichiro Takashima, Shinji Kakei, Ken-Ichiro Tsutsui, Toshio Iijima

    Neuroscience Research　57　(4)　579-586　2007/04

    DOI： 10.1016/j.neures.2007.01.003 　

    ISSN： 0168-0102

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Presentations 10

  1. Monkeys recognize the body image on the Virtual Reality as their own body through training

    Shinya Nakamura, Taro Takane, Daisuke Tajima, Hiroki Uchida, Ken-Ichiro Tsutsui, Shunichi Kasahara

    2023/09/15

  2. Exploring neural mechanisms of emotion regulation by using repetitive transcranial magnetic stimulation in monkeys

    Shinya Nakamura

    2023/08/02

  3. 腹内側前頭皮質への低頻度反復経頭蓋磁気刺激がサルの社交性に与える影響

    中村 晋也, 中村 元昭, 筒井 健一郎

    NEURO2022

  4. サル内側前頭皮質への低頻度反復経頭蓋磁気刺激はうつ様状態を誘起する

    中村晋也, 筒井健一郎

    第99回日本生理学会大会

  5. 細胞種特異的分子マーカーと神経トレーサーを用いたマカクザル嗅内皮質の構造解析

    吉野倫太郎, 川村太一, 中村晋也, 木村慧, 田辺創思, Andi Zheng, 井上謙一, 高田昌彦, Menno Witte, 筒井健一郎, 大原慎也

    第44回日本神経科学大会

  6. Subregions of medial frontal cortex project differently to nucleus accumebns and amygdala in macaque monkey

    Rintaro Yoshino, Kei Kimura, Soshi Tanabe, Shinya Ohara, Shinya Nakamura, Ken-Ichi Inoue, Masahiko Takada

    The 43rd Annual Meeting of the Japan Neuroscience Society　2020/07/31

  7. Neural mechanisms of emotional and social behavior in the monkey frontal cortex: a TMS study

    Shinya Nakamura

    The 43rd Annual Meeting of the Japan Neuroscience Society　2020/07/30

  8. Differential effects of rTMS to medial frontal and dorsolateral prefrontal cortices on competitive food picking behavior in monkeys

    Yoshiaki Ikeda, Takayuki Hosokawa, Hitoshi Nagano, Atsuhiro Saita, Shinya Nakamura, Kenichiro Tsutsui

    2019/07/26

  9. Effect of single-pulse transcranial magnetic stimulation on local neural activity in the primary motor cortex and its relation to motor evoked potential (MEP) examined by simultaneously recordings of electrocorticogram (ECoG) and MEP in monkeys.

    Yasutaka Honda, Shinya Nakamura, Kentaro Ogawa, Toshio Iijima, Yukio Nishimura, Ken-Ichiro Tsutsui

    The 42nd Annual Meeting of the Japan Neuroscience Society　2019/07/27

  10. Behavioral and physiological measures reflecting positive and negative emotions elicited by conditioned stimuli in a probabilistic Pavlovian conditioning task

    Shinya Nakamura, Toshiki Moritani, Yasutaka Honda, Ken-Ichiro Tsutsui

    The 42nd Annual Meeting of the Japan Neuroscience Society　2019/07/27

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Research Projects 1

  1. 反復経頭蓋磁気刺激による脳の長期的な可塑性誘導を支える神経生理学的基盤の解明

    中村 晋也

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2021/04 - 2024/03

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    多点硬膜下皮質表面電位（ECoG）記録のためのシート電極を埋め込んだサルの一次運動野（M1）に反復経頭蓋磁気刺激（rTMS）を施し、その前後において、脳活動（安静時ECoG）を経時的に記録するとともに、M1への単発TMSにより誘発される筋電位変化（運動誘発電位：MEP）をM1の機能の指標として同時記録することで、rTMSによって引き起こされる脳機能変化とそれにともなう脳活動変化の関係を探る実験を行った。 rTMSによって脳の可塑的変化を誘導するための最適な刺激頻度を調べるために、0.5から10Hzまでの5つの刺激頻度を用いて実験を行った。その結果、1HzでMEPの振幅の著しい減弱が、10HzでMEPの振幅の著しい増強が認められたことから、これらの刺激頻度がそれぞれ、rTMSによる脳機能の抑制と増強に適していることが示唆された。また、この時、1Hzでは安静時ECoGのβ帯域のパワーの減弱が、10HzではHigh-γ帯域のパワーの増強が認められたことから、これらの脳機能の抑制・増強には異なる神経メカニズムが関与していることが示唆された。さらに、単発TMSによって誘発されるECoG変化（TMS誘発電位）には、刺激から極短時間のうちにMEPの発生に関連した特徴的な電位変化が観察されており、その関係性について解析を進めている。rTMSを複数セッション行うことによって引き起こされる重畳効果についても、これらの指標の変化を総合的に評価することで検討を行っている。