Details of the Researcher

PHOTO

Takashi Nishioka
Section
Graduate School of Dentistry
Job title
Senior Assistant Professor
Degree

  • 博士（歯学）（東北大学）

e-Rad No.
50641875

Research History 4

  • 2021/08 - Present
    Tohoku University Graduate School of Dentistry　Lecturer

  • 2012/12 - 2021/07
    Tohoku University　Graduate School of Dentistry

  • 2012/06 - 2012/11
    東北大学病院　医員

  • 2008/10 - 2012/05
    Harvard Medical School, Beth Israel Deaconess Medical Center　Radiation Oncology

Committee Memberships 3

  • 日本歯科医学教育学会　代議員

    2025/07 - Present

  • 日本口腔診断学会　代議員

    2025/02 - Present

  • 公益社団法人 医療系大学間共用試験実施評価機構　歯学系臨床参加型臨床実習後客観的臨床能力試験 認定評価者小委員会（評価者等認定小委員会） 委員

    2020/04 - Present

Professional Memberships 8

  • Japanese Association for Disaster Medicine

  • JAPANESE SOCIETY FOR ORAL MEDICINE

  • JAPANESE DENTAL EDUCATION ASSOCIATION

  • Japan Endotoxin and Innate Immunity Society

  • JAPANESE ASSOCIATION FOR ORAL BIOLOGY

  • THE JAPANESE SOCIETY OF CONSERVATIVE DENTISTRY

  • The Japanese Society of Oral Diagnosis / Oral Medicine

  • JAPANESE SOCIETY FOR ORAL AND MAXILLOFACIAL RADIOLOGY

︎Show all ︎Show first 5

Research Areas 1

  • Life sciences / Oral medicine (pathology) /

Awards 1

  1. 東北開発記念財団海外派遣

    2013

Papers 47

  1. Lingual nerve revisited-A comprehensive review Part II: Surgery and radiology International-journal Peer-reviewed

    Kisho Ono, Takashi Nishioka, Kyoichi Obata, Yohei Takeshita, Chista Irani, Yuki Kunisada, Norie Yoshioka, Soichiro Ibaragi, R Shane Tubbs, Joe Iwanaga

    <B><I>Clinical anatomy<I><B>　2024/08/09

    DOI： 10.1002/ca.24211 　

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    The lingual nerve (LN) is a branch of the mandibular division of the fifth cranial nerve, the trigeminal nerve, arising in the infratemporal fossa. It provides sensory fibers to the mucous membranes of the floor of the mouth, the lingual gingiva, and the anterior two-thirds of the tongue. Although the LN should rarely be encountered during routine and basic oral surgical procedures in daily dental practice, its anatomical location occasionally poses the risk of iatrogenic injury. The purpose of this section is to consider this potential LN injury risk and to educate readers about the anatomy of this nerve and how to treat it.

  2. Neutrophil extracellular traps inhibit osteoclastogenesis Peer-reviewed

    Kento Numazaki, Hiroyuki Tada, Takashi Nishioka, Eiji Nemoto, Kenji Matsushita, Itaru Mizoguchi, Shunji Sugawara

    <B>Biochemical and Biophysical Research Communications<B>　705　149743　2024/02

    DOI： 10.1016/j.bbrc.2024.149743 　

  3. Macrophage migration inhibitory factor-mediated mast cell extracellular traps induce inflammatory responses upon Fusobacterium nucleatum infection Peer-reviewed

    Hiroyuki Tada, Takashi Nishioka, Rina Ishiyama, Li-Ting Song, Sakura Onoue, Kazuyoshi Kawahara, Eiji Nemoto, Kenji Matsushita, Shunji Sugawara

    <B>Biochemical and Biophysical Research Communications<B>　674　90-96　2023/06

    DOI： 10.1016/j.bbrc.2023.06.060 　

  4. Questionnaire survey on the satisfaction of SimEx dental education system Peer-reviewed

    Guang Hong, Chun Cheng Hung, Gen Mayanagi, Takashi Nishioka, Lu Sun, Eddie Hsiang Hua Lai, Ting Hsun Lan, Keiichi Sasaki, Nobuhiro Takahashi

    <B>Journal of Dental Sciences<B>　18　(2)　840-847　2023/04

    DOI： 10.1016/j.jds.2023.01.028 　

    ISSN： 1991-7902

    eISSN： 2213-8862

  5. Root canal narrowing patterns of mandibular first premolars on panoramic radiographs according to the number of root canals diagnosed on cone beam computed tomographic images. Peer-reviewed

    Yusuke Shimada, Ikuho Kojima, Takashi Nishioka, Maya Sakamoto, Masahiro Iikubo

    Odontology　111　(1)　172-177　2023/01

    DOI： 10.1007/s10266-022-00713-9 　

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    Understanding the variations in root canal morphology is important for successful endodontic treatment. Mandibular first premolars have a single root in 98% of cases, and multiple root canals are found in approximately 20% of cases. However, the details of these characteristic findings in panoramic radiography and their diagnostic accuracy are unknown. This study aimed to investigate the characteristic radiographic findings of mandibular first premolars possessing multiple root canals and determine the diagnostic accuracy using panoramic radiography. Four radiologists evaluated 347 mandibular first premolars in 186 patients who underwent panoramic radiography and dental cone beam computed tomography (CBCT). Two radiologists confirmed the existence of single or multiple root canals using CBCT. The remaining two radiologists evaluated the imaging findings of the root canal morphology and classified them into two patterns: gradual narrowing and sudden narrowing. In the case of the sudden narrowing pattern, the location of sudden narrowing was also evaluated. A gradual narrowing pattern was observed in 56% of teeth with a single root canal, and a sudden narrowing pattern was observed in 83% of teeth with multiple root canals. When sudden narrowing between the cervical and apical 1/4 of the root canal was used as the diagnostic criterion for multiple root canal teeth, the highest diagnostic performance was observed (sensitivity, 76.1%; specificity, 73.9%). The sudden narrowing between the cervical level and the apical 1/4 level of the root canal is a practical diagnostic criterion for mandibular first premolar with multiple root canals.

  6. Hericium erinaceus ethanol extract and ergosterol exert anti-inflammatory activities by neutralizing lipopolysaccharide-induced pro-inflammatory cytokine production in human monocytes International-journal Peer-reviewed

    Hiroyuki Tada, Kazuyoshi Kawahara, Hiraku Osawa, Li-Ting Song, Kento Numazaki, Junya Kawai, Sakura Onoue, Takashi Nishioka, Eiji Nemoto, Kenji Matsushita, Shunji Sugawara

    <B>Biochemical and Biophysical Research Communications<B>　636　(2)　1-9　2022/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbrc.2022.10.090 　

    ISSN： 0006-291X

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    Edible mushrooms are known to exert anti-inflammatory effects. In this study, the effects of ethanol extracts from edible mushrooms, such as Hericium erinaceus, and other edible mushrooms on inflammatory responses were investigated. Experiments were conducted using the inflammatory responses of human monocytes induced by lipopolysaccharide (LPS), a bacterial component, that provokes inflammation. Notably, we demonstrated that LPS mixed with ethanol and hot water extracts derived from edible mushrooms attenuated the production of inflammatory cytokines, such as interleukin (IL)-1β, -6, and -8, induced by LPS in human monocytic cell cultures. Moreover, we found that the ethanol extract of H. erinaceus contained ergosterol, which attenuated IL-8 production in LPS-stimulated cells. Subsequent component analysis of the ethanol extract of H. erinaceus revealed that ergosterol binds to lipid A to attenuate LPS-induced inflammation. Together, our findings suggest that ergosterol in ethanol extracts from edible mushrooms can prevent the induction of inflammation by binding to LPS.

  7. Mapping of dental graduates’ career paths in Hong Kong, Japan and mainland China Peer-reviewed

    Chloe Meng Jiang, Takashi Nishioka, Guang Hong, Hao Yu, Chang-Yuan Zhang, Chun Hung Chu

    <B>Frontiers in Oral Health<B>　3　994613　2022/11

    DOI： 10.3389/froh.2022.994613 　

    eISSN： 2673-4842

  8. PREVALENCE OF ALTERED TASTE AMONG THE ELDERLY IN BANGKOK, THAILAND Peer-reviewed

    Suteepichetpun I, Puengsurin D, Nishioka T, Satoh-Kuriwada S, Shoji N, Surarit R, Kiattavorncharoen S

    <B>Southeast Asian Journal of Tropical Medicine and Public Health<B>　53　(5)　457-468　2022/09

  9. COVID-19-Related Symptoms during the SARS-CoV-2 Omicron (B.1.1.529) Variant Surge in Japan. Peer-reviewed

    Tetsuya Akaishi, Shigeki Kushimoto, Yukio Katori, Noriko Sugawara, Hiroshi Egusa, Kaoru Igarashi, Motoo Fujita, Shigeo Kure, Shin Takayama, Michiaki Abe, Akiko Kikuchi, Minoru Ohsawa, Kota Ishizawa, Yoshiko Abe, Hiroyuki Imai, Yohei Inaba, Yoko Iwamatsu-Kobayashi, Takashi Nishioka, Ko Onodera, Tadashi Ishii

    The Tohoku journal of experimental medicine　258　(2)　103-110　2022/09

    DOI： 10.1620/tjem.2022.J067 　

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    The exact profiles of the clinical symptoms related to the SARS-CoV-2 Omicron variant (B.1.1.529) remain largely uncertain. Therefore, this study aimed to clarify the clinical manifestations of infection with this variant. We enrolled individuals who were tested by quantitative nasopharyngeal swab reverse transcription-polymerase chain reaction (RT-PCR) test at a large screening center in a city of Japan during the B.1.1.529 Omicron variant wave between January and May 2022, after contact with COVID-19 patients. Swab tests were planned to be performed approximately 4-5 days after contact. The presence of COVID-19-related symptoms was assessed at the swab test site. Among the 2,507 enrolled individuals, 943 (37.6%) were RT-PCR test-positive and 1,564 (62.4%) were test-negative. Among the 943 PCR test-positive participants, the prevalence of the symptoms was as follows: 47.3% with cough, 32.9% with sore throat, 18.4% with fatigability, 12.7% with fever of ≥ 37.5℃, 9.9% with dyspnea, 2.1% with dysosmia, and 1.4% with dysgeusia. The prevalence of cough, sore throat, dyspnea, and fatigability was higher among adults aged ≥ 18 years than among children and adolescents. The prevalence of dysosmia and dysgeusia remarkably decreased during the Omicron wave (1-3%) compared to during the pre-Omicron variant waves (15-25%). In summary, common COVID-19-related symptoms during the Omicron variant wave included cough and sore throat, followed by fatigability, fever, and dyspnea. The prevalence of most of these symptoms was higher in adults than in non-adults. The prevalence of dysosmia and dysgeusia remarkably decreased with the Omicron variant than with pre-Omicron variants.

  10. Effectiveness of third vaccine dose for coronavirus disease 2019 during the Omicron variant pandemic: a prospective observational study in Japan Peer-reviewed

    Tetsuya Akaishi, Shigeki Kushimoto, Yukio Katori, Noriko Sugawara, Hiroshi Egusa, Kaoru Igarashi, Motoo Fujita, Shigeo Kure, Shin Takayama, Michiaki Abe, Akiko Kikuchi, Minoru Ohsawa, Kota Ishizawa, Yoshiko Abe, Hiroyuki Imai, Yohei Inaba, Yoko Iwamatsu-Kobayashi, Takashi Nishioka, Ko Onodera, Tadashi Ishii

    Scientific Reports　12　(1)　13589　2022/08

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-022-17990-7 　

    eISSN： 2045-2322

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    Abstract The administration of a third booster dose of messenger ribonucleic acid (mRNA) vaccines against coronavirus disease 2019 (COVID-19) has progressed worldwide. Since January 2022, Japan has faced a nationwide outbreak caused by the Omicron variant, which occurred simultaneously with the progression of mass vaccination with the third booster dose. Therefore, this study evaluated the effectiveness of the third dose of vaccine by reverse transcription-polymerase chain reaction (RT-PCR) test using nasopharyngeal swab samples from adults aged ≥ 18 years tested after having close contact with COVID-19 cases between January and May 2022. Participants who completed only one dose were excluded from the study. Among the 928 enrolled participants, 139 had never been vaccinated, 609 had completed two doses, 180 had completed three doses before the swab test, and the overall RT-PCR test positivity rate in each group was 48.9%, 46.0%, and 32.2%, respectively. The vaccine effectiveness of the third dose to prevent infection after close contact was approximately 40% (95% confidence interval: 20–60%), which was the highest at 10–70 days after receiving the third dose. In conclusion, the effectiveness of the three-dose mRNA COVID-19 vaccine after close contact during the Omicron outbreak is approximately 40%.

  11. Effectiveness of an actuator-driven pulsed water jet for removal of softened carious dentin. Peer-reviewed

    Yuka Yokoyama-Sato, Takashi Nishioka, Yukihiro Naganuma, Masatoshi Takahashi, Atsuhiro Nakagawa, Nobuhiro Yoda, Keiichi Sasaki, Nobuhiro Takahashi, Teiji Tominaga, Masahiro Iikubo

    Dental materials journal　41　(4)　527-533　2022/07

    DOI： 10.4012/dmj.2021-234 　

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    The aim of this study was to assess the utility of an actuator-driven pulsed water jet (ADPJ) as a hardness-dependent carious dentin removal device by using different outputs. Thirty-six plane surface dental caries samples were treated with the ADPJ at 150, 200, and 250 voltage (12 teeth each). The Knoop hardness number (KHN) and Ca/P mass ratio were measured at 70 μm from the deepest point of the removing groove. Furthermore, three other teeth samples were manually treated with the ADPJ at the three above mentioned voltages (one tooth each) for 1 min. The KHN and Ca/P mass ratio were measured at 70 μm from the surface of the residual dentin part. In both the KHN and Ca/P mass ratio, higher residual dentin depended on the applied voltage of ADPJ. The ADPJ enabled the removal of softened carious dentin in an applied voltage-dependent manner.

  12. Porphyromonas gingivalis Gingipains-Mediated Degradation of Plasminogen Activator Inhibitor-1 Leads to Delayed Wound Healing Responses in Human Endothelial Cells. International-journal Peer-reviewed

    Li-Ting Song, Hiroyuki Tada, Takashi Nishioka, Eiji Nemoto, Takahisa Imamura, Jan Potempa, Chang-Yi Li, Kenji Matsushita, Shunji Sugawara

    Journal of innate immunity　14　(4)　306-319　2022/07

    DOI： 10.1159/000519737 　

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    Plasminogen activator inhibitor-1 (PAI-1), a serine protease inhibitor, is constitutively produced by endothelial cells and plays a vital role in maintaining vascular homeostasis. Chronic periodontitis is an inflammatory disease characterized by bleeding of periodontal tissues that support the tooth. In this study, we aimed to determine the role of PAI-1 produced by endothelial cells in response to infections caused by the primary periodontal pathogen Porphyromonas gingivalis. We demonstrated that P. gingivalis infection resulted in significantly reduced PAI-1 levels in human endothelial cells. This reduction in PAI-1 levels could be attributed to the proteolysis of PAI-1 by P. gingivalis proteinases, especially lysine-specific gingipain-K (Kgp). We demonstrated the roles of these degradative enzymes in the endothelial cells using a Kgp-specific inhibitor and P. gingivalis gingipain-null mutants, in which the lack of the proteinases resulted in the absence of PAI-1 degradation. The degradation of PAI-1 by P. gingivalis induced a delayed wound healing response in endothelial cell layers via the low-density lipoprotein receptor-related protein. Our results collectively suggested that the proteolysis of PAI-1 in endothelial cells by gingipains of P. gingivalis might lead to the deregulation of endothelial homeostasis, thereby contributing to the permeabilization and dysfunction of the vascular endothelial barrier.

  13. Reactivation of p53 by RITA Induces Apoptosis in Human Oral Squamous Cell Carcinoma Cells. International-journal Peer-reviewed

    Manabu Endo, Takashi Nishioka, Kento Numazaki, Hiroshi Hasegawa, Tetsu Takahashi, Shunji Sugawara, Hiroyuki Tada

    Anticancer research　42　(6)　2931-2937　2022/06

    DOI： 10.21873/anticanres.15775 　

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    BACKGROUND/AIM: Oral squamous cell carcinoma (OSCC) is one of the most common tumors of the head and neck region. The tumor suppressor gene p53 (TP53) is the most frequently mutated gene in OSCC and TP53 mutations are associated with decreased survival and resistance to chemotherapy in patients with OSCC. Therefore, therapeutic strategies targeting TP53 reactivation are required to effectively treat OSCC. In this study, we investigated the effect of various p53-reactivating small molecules (RITA, PRIMA-1, and CP-31398) on the proliferation of human OSCC cell lines (Ca9-22, HSC-2, HSC-3, and HSC-4) derived from human oral tissues bearing a mutant TP53 gene. MATERIALS AND METHODS: Apoptosis induction by RITA was assessed by measuring Annexin V and propidium iodide (PI)-positive cells using flow cytometry. p53 and murine double minute 2 (MDM2) phosphorylation and Bax expression were detected in the lysates of RITA-treated Ca9-22 cells using western blotting. RESULTS: RITA markedly inhibited the growth of Ca9-22, HSC-2, HSC-3, and HSC-4 cells. In Ca9-22 cells, RITA induced apoptosis and inhibited cell proliferation while increasing p53 phosphorylation and Bax expression; however, RITA did not induce MDM2 phosphorylation. CONCLUSION: The inhibitory effect of RITA on human OSCC cell proliferation is mediated by apoptosis induction through p53 and Bax.

  14. Effectiveness of mRNA COVID-19 Vaccines in Japan during the Nationwide Pandemic of the Delta Variant. Peer-reviewed

    Tetsuya Akaishi, Shigeki Kushimoto, Yukio Katori, Noriko Sugawara, Kaoru Igarashi, Motoo Fujita, Shigeo Kure, Shin Takayama, Michiaki Abe, Akiko Kikuchi, Kota Ishizawa, Yoshiko Abe, Hiroyuki Imai, Yohei Inaba, Yoko Iwamatsu-Kobayashi, Takashi Nishioka, Ko Onodera, Tadashi Ishii

    The Tohoku journal of experimental medicine　257　(1)　1-6　2022/05/13

    DOI： 10.1620/tjem.2022.J012 　

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    The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remained a major global health concern in 2021. To suppress the spread of infection, mass vaccinations have been performed across countries worldwide. In Japan, vaccinations of the first and second doses for most of the nation were performed during the nationwide outbreak of the B.1.617.2 (Delta) variant with the L452R spike protein mutation, and the effectiveness of the vaccinations to suppress the spread of COVID-19 among the people in Japan remains uncertain. In this study, adults aged ≥18 years, who were in contact with patients with COVID-19 and underwent nasopharyngeal swab reverse transcription-polymerase chain reaction (RT-PCR) tests during August and September 2021 at a mass screening test center in Japan, were enrolled. In this period, more than 95% of the COVID-19 infections were reportedly caused by the Delta variant. As a result, a total of 784 adults with recent contact history, including 231 (29.5%) RT-PCR test-positive cases, were enrolled. The test positivity rate was lower in individuals who had been vaccinated twice than in unvaccinated individuals (12.5% vs. 39.0%, p < 0.0001), with the risk ratio of 0.32 (95% confidence interval 0.23-0.46). The vaccine effectiveness was the highest between 7-90 days after the second vaccine dose. In conclusion, two doses of mRNA COVID-19 vaccines effectively suppressed transmission in Japan during the nationwide pandemic of the Delta variant, estimated to have prevented 50-80% of the infection.

  15. Impact of the COVID‐19 pandemic on dental clinical training and future prospects International-journal Peer-reviewed

    Takashi Nishioka, Gen Mayanagi, Yoko Iwamatsu‐Kobayashi, Guang Hong

    Journal of Dental Education　85　(3)　1999-2001　2021/12

    Publisher: Wiley

    DOI： 10.1002/jdd.12562 　

    ISSN： 0022-0337

    eISSN： 1930-7837

  16. COVID-19 Transmission at Schools in Japan. Peer-reviewed

    Tetsuya Akaishi, Shigeki Kushimoto, Yukio Katori, Noriko Sugawara, Kaoru Igarashi, Motoo Fujita, Shigeo Kure, Shin Takayama, Michiaki Abe, Junichi Tanaka, Akiko Kikuchi, Yoshiko Abe, Hiroyuki Imai, Yohei Inaba, Yoko Iwamatsu-Kobayashi, Takashi Nishioka, Ko Onodera, Tadashi Ishii

    The Tohoku journal of experimental medicine　255　(3)　239-246　2021/11

    DOI： 10.1620/tjem.255.239 　

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    The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains a global public health concern in 2021. However, the risk of attending schools during the pandemic remains unevaluated. This study estimated the secondary transmission rate at schools using the results of a real-time reverse transcription-polymerase chain reaction (RT-PCR) screening test performed between July 2020 and April 2021, before starting the nationwide mass vaccination. A total of 1,924 students (20 RT-PCR-positive; 1.0%) from 52 schools or preschools were evaluated, together with 1,379 non-adults (95 RT-PCR-positive; 6.9%) exposed to SARS-CoV-2 in non-school environments. Assuming that the infectious index cases were asymptomatic and the transmission at schools followed a Bernoulli process, we estimated the probability of transmission after each contact at school as approximately 0.005 (0.5% per contact) with the current infection prevention measures at schools in Japan (i.e., hand hygiene, physical distancing, wearing masks, and effective ventilation). Furthermore, assuming that all children are capable of carrying the infection, then contact between an index case and 20-30 students per day at schools would yield the expected value for secondary cases of ≥ 1.0, during the 10 days of the infectious period. In conclusion, with the current infection prevention measures at schools in Japan, secondary transmission at schools would occur in approximately every 200 contacts. When considering this rate, compliance with the current infection prevention measures at schools and early detection and quarantine of the index cases would be effective in preventing the spread of COVID-19 at schools.

  17. Discriminatory Value of Self-reported Olfactory Dysfunction in the Prediction of Coronavirus Disease 2019 Peer-reviewed

    Tetsuya Akaishi, Shigeki Kushimoto, Yukio Katori, Shigeo Kure, Kaoru Igarashi, Motoo Fujita, Shin Takayama, Michiaki Abe, Akiko Kikuchi, Junichi Tanaka, Yoshiko Abe, Hiroyuki Imai, Yohei Inaba, Yoko Iwamatsu-Kobayashi, Takashi Nishioka, Ko Onodera, Tadashi Ishii

    Internal Medicine　60　(18)　2905-2910　2021/09

    Publisher: Japanese Society of Internal Medicine

    DOI： 10.2169/internalmedicine.7238-21 　

    ISSN： 0918-2918

    eISSN： 1349-7235

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    Objective Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), remains the world's largest public health concern in 2021. A history of close contact with infectious patients is a factor that predicts a positive SARS-CoV-2 test result. Meanwhile, the precise predictive value of symptoms suggestive of COVID-19 has not been fully elucidated. This study aimed to clarify the predictive and discriminatory value of each clinical symptom suggestive of COVID-19. Methods This study enrolled participants who were tested for SARS-CoV-2 by reverse transcription polymerase chain reaction using a nasopharyngeal swab between November 2020 and January 2021. All enrolled patients were evaluated for data regarding the presence and closeness of contact with infectious patients and comprehensive clinical features (i.e., fever, cough, dyspnea, fatigue, dysosmia, and dysgeusia). Results Among the 1,744 tested participants, 144 tested positive for SARS-CoV-2. In the test-positive group, self-reported cough, fatigue, dysosmia, and dysgeusia were significant predictors of COVID-19, independent from a history of close contact. In particular, the presence of dysosmia was the strongest predictor of COVID-19 in both univariate and multivariate analyses. Among the 42 patients with self-reported dysosmia, 25 (59.5%) were SARS-CoV-2 test-positive. Self-reported dysosmia was reported by 25 (17.4%) of the 144 patients who tested positive for SARS-CoV-2, and 15 (60.0%) of the 25 COVID-19 patients with dysosmia had accompanying dysgeusia. Conclusion The presence of dysosmia was reported by 10-25% of patients with COVID-19, and is a significant predictor of COVID-19 infection, independent from a history of close contact.

  18. Impacts of Natural Environmental Factors and Prevalence of Airway Symptoms on the Local Spread of COVID-19: A Time-Series Analysis in Regional COVID-19 Epidemics Peer-reviewed

    Tadashi Ishii, Shigeki Kushimoto, Yukio Katori, Shigeo Kure, Kaoru Igarashi, Motoo Fujita, Noriko Sugawara, Shin Takayama, Michiaki Abe, Junichi Tanaka, Akiko Kikuchi, Yoshiko Abe, Hiroyuki Imai, Yohei Inaba, Yoko Iwamatsu-Kobayashi, Takashi Nishioka, Ko Onodera, Tetsuya Akaishi

    The Tohoku Journal of Experimental Medicine　254　(2)　89-100　2021/06

    Publisher: Tohoku University Medical Press

    DOI： 10.1620/tjem.254.89 　

    ISSN： 0040-8727

    eISSN： 1349-3329

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    Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the world's largest public health concern in 2021. This study evaluated the associations of the prevalence of airway symptoms among the tested individuals and data regarding the natural environmental factors with the weekly number of newly diagnosed COVID-19 patients in Sendai City (Nt). For the derivatives of the screening test results, data from individuals with a contact history who underwent nasopharyngeal swab reverse transcription-polymerase chain reaction (RT-PCR) testing between July 2020 and April 2021 (6,156 participants, including 550 test-positive patients) were used. The value of Nt correlated with the weekly RT-PCR test-positive rate after close contact, prevalence of cough symptoms in test-positive individuals or in test-negative individuals, lower air temperature, lower air humidity, and higher wind speed. The weekly test-positive rate correlated with lower air humidity and higher wind speed. In cross-correlation analyses, natural environmental factors correlated with the regional epidemic status on a scale of months, whereas the airway symptoms among non-COVID-19 population affected on a scale of weeks. When applying an autoregression model to the serial data of Nt, large-scale movements of people were suggested to be another factor to influence the local epidemics on a scale of days. In conclusion, the prevalence of cough symptoms in the local population, lower air humidity or higher wind speed, and large-scale movements of people in the locality would jointly influence the local epidemic status of COVID-19.

  19. Perturbation of cellular integrity by nicotine: A major component of e-cigarette smoke Peer-reviewed

    Suthakar Ganapathy, Qiang Zhang, Takashi Nishioka, Alexandros Makriyannis, Changyan Chen

    Journal of Cancer Biology　2　(1)　10-14　2021/02/03

    Publisher: ProBiologists LLC

    DOI： 10.46439/cancerbiology.2.017 　

  20. Predictors of SARS-CoV-2 Positivity based on RT-PCR Swab Tests at a Drive-Through Outpatient Clinic for COVID-19 Screening in Japan Peer-reviewed

    Ishii T, Kushimoto S, Katori Y, Kure S, Igarashi K, Fujita M, Takayama S, Abe M, Tanaka J, Kikuchi A, Abe Y, Imai H, Inaba Y, Iwamatsu-Kobayashi Y, Nishioka T, Onodera K, Akaishi T

    The Tohoku Journal of Experimental Medicine　253　(2)　101-108　2021/02

    DOI： 10.1620/tjem.253.101 　

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    In response to the COVID-19 pandemic caused by SARS-CoV-2 in 2020, we conducted drive-through nasopharyngeal swab testing for COVID-19 in Sendai city, Japan, since April 2020. All tested individuals were judged in advance by public health centers for the necessity of undergoing the test with possible contact history and/or symptoms suggestive of COVID-19. In this study, to identify the predictors of SARS-CoV-2 test positivity for more efficient and evidenced selection of suspected individuals, we enrolled 3,540 consecutive individuals, tested in the first 7 months of the testing program, with data regarding to the history of close contact with COVID-19 patients, including those involved in cluster outbreaks. This cohort included 284 foreign students (257 males and 27 females) from a vocational school involved in the largest cluster outbreak in the area. Close contact history was present in 952 (26.9%) of the participants. The reverse transcription-polymerase chain reaction (RT-PCR) test results showed that 164 participants (4.6%) were positive and 3,376 participants (95.4%) were negative for the SARS-CoV-2 nucleocapsid gene (N2). In the univariate and multivariate analyses, history of close contact with COVID-19 patients, higher age, cough symptoms, and non-native ethnicity were predictors for SARS-CoV-2 test positivity. However, the significance of age and foreign nationality disappeared or declined upon excluding the foreign students from the aforementioned largest cluster outbreak. In conclusion, a history of close contact with COVID-19 patients and the presence of cough symptoms are significant predictors of SARS-CoV-2 test positivity.

  21. Effect of exposure parameters and gutta-percha cone size on fracture-like artifacts in endodontically treated teeth on cone-beam computed tomography images. Peer-reviewed

    Iikubo M, Kagawa T, Fujisawa J, Kumasaka A, Nishioka T, Kojima I, Sakamoto M, Kobayashi K, Yuasa K

    Oral radiology　36　(4)　344-348　2020/10

    DOI： 10.1007/s11282-019-00411-3 　

    ISSN： 0911-6028

    eISSN： 1613-9674

  22. Florid Cemento-Osseous Dysplasia-Associated Simple Bone Cyst Showing Marked Irregular Border and High Apparent Diffusion Coefficient Value. International-journal Peer-reviewed

    Ikuho Kojima, Takashi Nishioka, Maya Sakamoto, Yuko Sai, Yushi Ezoe, Masahiro Iikubo, Hiroyuki Kumamoto, Tetsu Takahashi

    Case reports in dentistry　2020　8854428-8854428　2020/09

    DOI： 10.1155/2020/8854428 　

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    A simple bone cyst (SBC) is an intrabone cavity without epithelial lining, which occasionally occur with fibrous-osseous lesions. The imaging finding of the scalloped border and conserving the lamina dura, periodontal ligament spaces, or follicular spaces are considered radiographically pathognomonic of SBC. SBC has been reported to occur with fibrous-osseous lesions, including cemento-osseous dysplasia (COD). Computed tomography (CT) imaging findings are sparse, and there are no reports of magnetic resonance imaging (MRI) findings, including apparent diffusion coefficient (ADC) value calculated by diffusion-weighted MRI for the florid COD-associated SBC. We report a case of a 39-year-old woman who was referred to our hospital because a panoramic radiograph of her right mandible showed asymptomatic radiolucency in the apical molar area. CT images showed a low-density lesion in the periapical areas of the right mandible, which accompanied a well-defined, high-density lesion, and a mixed low/high-density lesion with an unusual marked irregular border in the left mandible. The MRI showed cyst-like extremely hyperintense signals on fat-suppressed T2-weighted images at the right mandibular low-density and the left mandibular mixed low/high-density areas. ADC map showed high values (over 2.5 × 10-3 mm2/s) at the cystic areas in the bilateral mandible. We performed incisional biopsies of the bilateral mandibular lesions and resections of the bilateral maxillary lesions. Surgical and histopathological findings established a diagnosis of florid COD in the bilateral mandible and maxilla, simultaneously complicated by multiple SBCs in the bilateral mandible. The ADC value of cystic component is useful for the diagnosis, if the COD-associated SBC exhibits solitary, nonspecific, or rare imaging findings.

  23. A case of parotid duct cyst mimicking benign tumor with cystic component Peer-reviewed

    Takashi Nishioka, Takenori Ogawa, Takaki Murata, Ikuho Kojima, Yusuke Shimada, Masahiro Iikubo, Maya Sakamoto

    Dental Radiology　60　(1)　24-26　2020/09

    Publisher:

    ISSN： 0389-9705

    eISSN： 2185-6311

  24. The effectiveness of an actuator-driven pulsed water jet for the removal of artificial dental calculus: a preliminary study Peer-reviewed

    Yuka Sato, Masahiro Iikubo, Takashi Nishioka, Nobuhiro Yoda, Tetsuya Kusunoki, Atsuhiro Nakagawa, Keiichi Sasaki, Teiji Tominaga

    BMC Oral Health　20　(1)　205　2020/07

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1186/s12903-020-01190-8 　

    eISSN： 1472-6831

  25. Nicotine exposure potentiates lung tumorigenesis by perturbing cellular surveillance Peer-reviewed

    Zhang Q, Ganapathy S, Avraham H, Nishioka T, Chen C

    British Journal of Cancer　122　(6)　904-911　2020/03

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41416-020-0730-0 　

    ISSN： 0007-0920

    eISSN： 1532-1827

  26. Porphyromonas gingivalis induces the production of interleukin-31 by human mast cells, resulting in dysfunction of the gingival epithelial barrier. International-journal Peer-reviewed

    Tada H, Nishioka T, Takase A, Numazaki K, Bando K, Matsushita K

    Cellular microbiology　21　(3)　e12972　2019/03

    DOI： 10.1111/cmi.12972 　

    ISSN： 1462-5814

    More details Close

    Interleukin (IL)-31 is important for innate immunity in mucosal tissues and skin, and increased IL-31 expression participates in the pathogenesis of chronic inflammatory diseases affecting the skin, airways, lungs, and intestines. We investigated the contribution of mast cells to the induction of IL-31 production following infection with the periodontal pathogen, Porphyromonas gingivalis. We found that oral infection with P. gingivalis increased IL-31 expression in the gingival tissues of wild-type mice but not in those of mast cell-deficient mice. The P. gingivalis-induced IL-31 production by human mast cells occurred through the activation of the JNK and NF-κB signalling pathways and was dependent on the P. gingivalis lysine-specific protease gingipain-K. P. gingivalis infection induced IL-31 receptor α and oncostatin M receptor β expression in human gingival epithelial cells. Notably, the P. gingivalis-induced IL-31 production by mast cells led to the downregulation of claudin-1, a tight junction molecule, in gingival epithelial cells, resulting in an IL-31-dependent increase in the paracellular permeability of the gingival epithelial barrier. These findings suggest that IL-31 produced by mast cells in response to P. gingivalis infection causes gingival epithelial barrier dysfunction, which may contribute to the chronic inflammation observed in periodontitis.

  27. Nicotine exposure induces the proliferation of oral cancer cells through the α7 subunit of the nicotinic acetylcholine receptor. International-journal Peer-reviewed

    Takashi Nishioka, Hiroyuki Tada, Soichiro Ibaragi, Changyan Chen, Takashi Sasano

    Biochemical and Biophysical Research Communications　509　(2)　514-520　2019/02

    DOI： 10.1016/j.bbrc.2018.12.154 　

    ISSN： 0006-291X

    More details Close

    Oral cancer and smoking are closely related, because the oral cavity, which is the route of ingestion of tobacco smoke, is in direct contact with the oral mucosa. Nicotine, one of the components of tobacco, can diffuse rapidly to the central nervous system and is responsible for tobacco addiction. Nicotine is present in high concentrations in the bloodstream of smokers; while the addictive effects of this alkaloid have extensively been studied, its effect on tumorigenesis is not clear yet. Therefore, in this study, we examined the effect of nicotine on cell proliferation and the signaling pathways it activates. The human oral squamous cell carcinoma cell line HSC-2 was used as a model system. We demonstrated the correlation between nicotine and epidermal growth factor receptor (EGFR) signaling. Nicotine treatment induced HSC-2 cell proliferation and migration and the phosphorylation of EGFR. Furthermore, nicotine treatment activated the EGFR downstream effectors phosphatidylinositol-3 kinase/AKT and p44/42 mitogen-activated protein kinases (ERK), which, in turn, promoted cell proliferation. Overall, our study suggests that nicotine promotes cell growth and migration through epidermal growth factor (EGF) signaling and plays an important role in oral cancer progression.

  28. Relationship of MR imaging of submandibular glands to hyposalivation in Sjögren's syndrome Peer-reviewed

    Ikuho Kojima, Maya Sakamoto, Masahiro Iikubo, Yusuke Shimada, Takashi Nishioka, Takashi Sasano

    Oral diseases　25　(1)　117-125　2019/01

    DOI： 10.1111/odi.12941 　

    ISSN： 1354-523X

    eISSN： 1601-0825

  29. Nicotine promotes lymph node metastasis and cetuximab resistance in head and neck squamous cell carcinoma. International-journal Peer-reviewed

    Rieko Shimizu, Soichiro Ibaragi, Takanori Eguchi, Daisuke Kuwajima, Shinichi Kodama, Takashi Nishioka, Tatsuo Okui, Kyoichi Obata, Kiyofumi Takabatake, Hotaka Kawai, Kisho Ono, Kuniaki Okamoto, Hitoshi Nagatsuka, Akira Sasaki

    International journal of oncology　54　(1)　283-294　2019/01

    DOI： 10.3892/ijo.2018.4631 　

    ISSN： 1019-6439

    More details Close

    Epidermal growth factor (EGF) is overexpressed in many cancers and is associated with worse prognosis. EGF binds to its cell surface receptor (EGFR), which induces EGFR phosphorylation. Phosphorylated EGFR (p‑EGFR) is translocated into the nucleus, which increases cancer cell activity. Nicotine, which is one of the main components of tobacco, is absorbed through pulmonary alveoli and mucosal epithelia in the head and neck region by smoking and moves into the blood. Nicotine in blood binds to nicotinic acetylcholine receptor (nAChR) in the central nervous system and serves a crucial role in tobacco addiction. Although nAChR localization is thought to be limited in the nervous system, nAChR is present in a wide variety of non‑neuronal cells, including cancer cells. Recent studies suggest that nicotine contributes to the metastasis and resistance to anti‑cancer drugs of various cancer cells. However, it remains unknown whether head and neck squamous cell carcinoma (HNSCC) cells can utilize nicotine‑nAChR signaling to metastasize and acquire resistance to anti‑cancer drugs, even though the mucosal epithelia of the head and neck region are the primary sites of exposure to tobacco smoke. To the best of our knowledge, the present study is the first to demonstrate the role of nicotine in metastasis and anti‑EGFR‑therapy resistance of HNSCC. The present findings demonstrated that nicotine increased proliferation, migration, invasion, p‑EGFR nuclear translocation and protein kinase B (Akt) phosphorylation in HNSCC cells. It was also demonstrated that nicotine restored cetuximab‑inhibited proliferation, migration and invasion of HNSCC cells. Finally, an in vivo experiment revealed that nicotine increased lymph node metastasis of xenografted tumors, whereas an nAChR inhibitor suppressed lymph node metastasis and p‑EGFR nuclear localization of xenografted tumors. Taken together, these results demonstrated that nicotine induced nuclear accumulation of p‑EGFR, and activation of Akt signaling. These signaling pathways elevated the activities of HNSCC cells, causing lymph node metastasis and serving a role in cetuximab resistance.

  30. P.gingivalis感染によりマスト細胞から産生されたIL-31はclaudin-1発現抑制を介して歯肉上皮バリア破綻を誘導する

    多田 浩之, 沼崎 研人, 西岡 貴志, 松下 健二, 菅原 俊二

    Journal of Oral Biosciences Supplement　2018　202-202　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  31. Influence of voxel size and scan field of view on fracture-like artifacts from gutta-percha obturated endodontically treated teeth on cone-beam computed tomography images Peer-reviewed

    Masahiro Iikubo, Takashi Nishioka, Shintaro Okura, Kaoru Kobayashi, Tsukasa Sano, Akitoshi Katsumata, Eiichiro Ariji, Ikuho Kojima, Maya Sakamoto, Takashi Sasano

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY　122　(5)　631-637　2016/11

    DOI： 10.1016/j.oooo.2016.07.014 　

    ISSN： 2212-4403

    eISSN： 1528-395X

  32. Porphyromonas gingivalis Gingipain-Dependently Enhances IL-33 Production in Human Gingival Epithelial Cells International-journal Peer-reviewed

    Hiroyuki Tada, Takashi Matsuyama, Takashi Nishioka, Makoto Hagiwara, Yusuke Kiyoura, Hidetoshi Shimauchi, Kenji Matsushita

    PLOS ONE　11　(4)　e0152794　2016/04

    DOI： 10.1371/journal.pone.0152794 　

    ISSN： 1932-6203

  33. Insulin-Like Growth Factor I Inhibits Alveolar Bone Loss Following Tooth Extraction in Rats Peer-reviewed

    Akira Kumasaka, Masahiro Iikubo, Takashi Nishioka, Ikuho Kojima, Noriaki Shoji, Maya Sakamoto, Takashi Sasano

    CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH　17　(6)　1174-1179　2015/12

    DOI： 10.1111/cid.12227 　

    ISSN： 1523-0899

    eISSN： 1708-8208

  34. Comparison of bisecting and parallel intraoral radiography and cone-beam computed tomography for detecting various horizontal angle root fractures Peer-reviewed

    Masahiro Iikubo, Takashi Kamio, Naoya Hashimoto, Takashi Nishioka, Mamoru Wakoh, Tsukasa Sano, Chinami Igarashi, Kaoru Kobayashi, Kenji Seki, Akitoshi Katsumata, Eiichiro Ariji, Takashi Sasano, Maya Sakamoto, Ikuho Kojima

    ORAL RADIOLOGY　31　(3)　173-180　2015/09

    DOI： 10.1007/s11282-015-0201-0 　

    ISSN： 0911-6028

    eISSN： 1613-9674

  35. Nicotine increases the resistance of lung cancer cells to cisplatin through enhancing Bcl-2 stability Peer-reviewed

    T. Nishioka, L-Y Luo, L. Shen, H. He, A. Mariyannis, W. Dai, C. Chen

    BRITISH JOURNAL OF CANCER　110　(7)　1785-1792　2014/04

    DOI： 10.1038/bjc.2014.78 　

    ISSN： 0007-0920

    eISSN： 1532-1827

  36. Increased Interleukin-18 in the Gingival Tissues Evokes Chronic Periodontitis after Bacterial Infection Peer-reviewed

    Kotaro Yoshinaka, Noriaki Shoji, Takashi Nishioka, Yumiko Sugawara, Tomoaki Hoshino, Shunji Sugawara, Takashi Sasano

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　232　(3)　215-222　2014/03

    DOI： 10.1620/tjem.232.215 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  37. Physiological Distal Drift in Rat Molars Contributes to Acellular Cementum Formation Peer-reviewed

    Shinobu Tsuchiya, Masahiro Tsuchiya, Takashi Nishioka, Osamu Suzuki, Yasuyuki Sasano, Kaoru Igarashi

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY　296　(8)　1255-1263　2013/08

    DOI： 10.1002/ar.22731 　

    ISSN： 1932-8486

    eISSN： 1932-8494

  38. Geminin Functions Downstream of p53 in K-ras-Induced Gene Amplification of Dihydrofolate Reductase Peer-reviewed

    Ling Shen, Takashi Nishioka, Jinjin Guo, Changyan Chen

    CANCER RESEARCH　72　(23)　6153-6162　2012/12

    DOI： 10.1158/0008-5472.CAN-12-1862 　

    ISSN： 0008-5472

  39. Nicotine Overrides DNA Damage-Induced G(1)/S Restriction in Lung Cells Peer-reviewed

    Takashi Nishioka, Daisuke Yamamoto, Tongbo Zhu, Jinjin Guo, Sung-Hoon Kim, Chang Yan Chen

    PLOS ONE　6　(4)　e18619　2011/04

    DOI： 10.1371/journal.pone.0018619 　

    ISSN： 1932-6203

  40. Sensitization of epithelial growth factor receptors by nicotine exposure to promote breast cancer cell growth. Peer-reviewed

    Nishioka T, Kim HS, Luo LY, Huang Y, Guo J, Chen CY

    Breast cancer research　13　(6)　R113　2011

    DOI： 10.1186/bcr3055 　

    ISSN： 1465-5411

  41. Ornithine Decarboxylase Antizyme Induces Hypomethylation of Genome DNA and Histone H3 Lysine 9 Dimethylation (H3K9me2) in Human Oral Cancer Cell Line Peer-reviewed

    Daisuke Yamamoto, Kaori Shima, Kou Matsuo, Takashi Nishioka, Chang Yan Chen, Guo-fu Hu, Akira Sasaki, Takanori Tsuji

    PLOS ONE　5　(9)　e12554　2010/09

    DOI： 10.1371/journal.pone.0012554 　

    ISSN： 1932-6203

  42. Differential sensitization of different prostate cancer cells to apoptosis. Peer-reviewed

    Guo J, Zhu T, Chen L, Nishioka T, Tsuji T, Xiao ZX, Chen CY

    Genes & cancer　1　(8)　836-846　2010/08

    DOI： 10.1177/1947601910381645 　

    ISSN： 1947-6019

  43. Phellinus Linteus Extract Sensitizes Advanced Prostate Cancer Cells to Apoptosis in Athymic Nude Mice Peer-reviewed

    Takanori Tsuji, Wei Du, Takashi Nishioka, Lihua Chen, Daisuke Yamamoto, Chang Yan Chen

    PLOS ONE　5　(3)　e9885　2010/03

    DOI： 10.1371/journal.pone.0009885 　

    ISSN： 1932-6203

  44. Nicotine, Through Upregulating Pro-Survival Signaling, Cooperates With NNK to Promote Transformation Peer-reviewed

    Takashi Nishioka, Jinjin Guo, Daisuke Yamamoto, Lihua Chen, Petra Huppi, Chang Yan Chen

    JOURNAL OF CELLULAR BIOCHEMISTRY　109　(1)　152-161　2010/01

    DOI： 10.1002/jcb.22392 　

    ISSN： 0730-2312

  45. Induction of serum IL-18 with Propionibacterium acnes and lipopolysaccharide in phagocytic macrophage-inactivated mice Peer-reviewed

    Takashi Nishioka, Toshinobu Kuroishi, Yumiko Sugawara, Zhiqian Yu, Takashi Sasano, Yasuo Endo, Shunji Sugawara

    JOURNAL OF LEUKOCYTE BIOLOGY　82　(2)　327-334　2007/08

    DOI： 10.1189/jlb.1006598 　

    ISSN： 0741-5400

    eISSN： 1938-3673

  46. A Case of Progressive Transformation of Germinal Centers with Multiple Swollen Cervical Lymph Nodes Peer-reviewed

    47　(1)　26-32　2007

  47. Involvement of neutrophil recruitment and protease-activated receptor 2 activation in the induction of IL-18 in mice Peer-reviewed

    K Ikawa, T Nishioka, ZQ Yu, Y Sugawara, J Kawagoe, T Takizawa, Primo, V, B Nikolic, T Kuroishi, T Sasano, H Shimauchi, H Takada, Y Endo, S Sugawara

    JOURNAL OF LEUKOCYTE BIOLOGY　78　(5)　1118-1126　2005/11

    DOI： 10.1189/jlb.0305151 　

    ISSN： 0741-5400

Show all ︎Show first 5

Misc. 54

  1. 災害医療人材教育におけるオンライン型実習の実際と課題

    菅野 武, 只野 恭教, 阿部 喜子, 藤田 基生, 西岡 貴志, 今井 浩之, 佐々木宏之, 江川 新一, 島田 二郎, 石井 正

    第28回日本災害医学会総会・学術集会　2023/03

  2. The Usefulness of the Novel Viscometer on Viscosity Measurement of Minor Salivary Glands: Pilot Study

    Boonnapa Temtanapa, Ittetsu Uchigasaki, Noriaki Shoji, Masashi Mizukami, Takashi Nishioka, Yoshinori Hattori, Masahiro Iikubo, Kazue Kurihara

    2022/12

  3. Neutrophil extracellular trapsのdouble-stranded DNAは破骨細胞分化を制御する

    沼崎研人, 西岡貴志, 松下健二, 多田浩之

    第27回日本エンドトキシン・自然免疫研究会　2022/11

  4. Porphyromonas gingivalisジンジパインはヒト血管内皮細胞のPAI-1を分解し，血管内皮の創傷治癒を遅延させる

    多田 浩之, 西岡 貴志, 松下 健二, 菅原 俊二

    第64回歯科基礎医学会学術大会　2022/09

  5. Panoramic radiographic features of mandibular first premolars with multiple root canals diagnosed on cone beam computed tomography

    Yusuke Shimada, Ikuho Kojima, Takashi Nishioka, Maya Sakamoto, Masahiro Iikubo

    The 13th Asian Congress of Oral and Maxillo-Facial Radiology & The 62nd general assembly and annual scientific congress of Japanese Society for Oral and Maxillo-Facial Radiology　2022/06

  6. Impact of the COVID-19 pandemic on dental clinical training in Tohoku University

    Takashi Nishioka

    2021/09/12

  7. パルスジェットを用いたう蝕軟化象牙質除去に関する研究

    佐藤由加, 西岡貴志, 長沼由泰, 依田信裕, 高橋正敏, 中川敦寛, 髙橋信博, 佐々木啓一, 冨永悌二, 飯久保正弘

    日本歯科放射線学会 第232回関東地方会・第40回北日本地方会・第28回合同地方会（2021年8月21日 Web開催）　2021/08

  8. ドライブスルー型新型コロナウイルス感染症検査外来の運用1 検査診療体制について

    稲葉洋平, 阿部喜子, 西岡貴志, 藤田基生, 石井正

    第26回日本災害医学会総会・学術集会　25　(3)　165-165　2021/03

    Publisher: (一社)日本災害医学会

    ISSN： 2189-4035

    eISSN： 2434-4214

  9. ドライブスルー型新型コロナウイルス感染症検査外来の運用2 ロジスティクスについて

    阿部喜子, 稲葉洋平, 西岡貴志, 藤田基生, 石井正

    第26回日本災害医学会総会・学術集会　25　(3)　166-166　2021/03

    Publisher: (一社)日本災害医学会

    ISSN： 2189-4035

    eISSN： 2434-4214

  10. 災害歯科保健医療研修に活用できる映像媒体の開発

    中久木康一, 西岡貴志, 鈴木敏彦, 洪光, 小坂健, 佐々木啓一

    第18回警察歯科医会全国大会　2021/02/06

  11. Color images showing the effective atomic number and electron density of the dentin caries using photon counting.

    飯久保正弘, 藤澤純也, 佐藤由加, 西岡貴志, 石幡浩志, 青木徹

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)　152nd　2020

  12. 耳下腺の非腫瘍性嚢胞様病変のMRI所見

    阪本真弥, 西岡貴志, 小川武則, 村田隆紀, 小嶋郁穂, 嶋田雄介, 飯久保正弘

    日本歯科放射線学会第24回臨床画像大会　2019/11

  13. 複数根管を有する下顎第一小臼歯のパノラマエックス線写真における特徴的画像所見の検討

    嶋田雄介, 小嶋郁穂, 西岡貴志, 飯久保正弘, 阪本真弥

    日本歯科放射線学会第24回臨床画像大会　2019/11

  14. ニコチンが口腔扁平上皮癌細胞における細胞周期やアポトーシスに与える影響

    西岡貴志, 伊原木聰一郎, 庄司憲明

    第12回日本口腔検査学会，第30回日本臨床口腔病理学会，第29回日本口腔内科学会，第32回日本口腔診断学会合同学術大会　32nd　2019/09

  15. Effect of exposure parameters on fracture-like artifacts of gutta-percha cones on cone-beam computed tomography images.

    Iikubo M, Kagawa T, Yuasa K, Kobayashi K, Fujisawa J, Sato Y, Kumasaka A, Nishioka T, Kojima I, Sakamoto M

    22th International Congress of Dental and Maxillofacial Radiology　2019/08

  16. Efficacy of the positron emission tomography scanner with semiconductor photomultiplier in diagnosis of lymph node metastases in oral cancer

    Kojima I, Iikubo M, Nishioka T, Sakamoto M

    22th International Congress of Dental and Maxillofacial Radiology　2019/08

  17. Porphyromonas gingivalisジンジパインによりマスト細胞が産生するIL-31は、歯肉上皮細胞のclaudin-1発現を抑制しバリア破綻を誘導する

    多田浩之, 西岡貴志, 沼崎研人, 根本英二, 松下健二, 菅原俊二

    第73回日本細菌学会東北支部会　2019/08

  18. パルスジェットを用いた歯の付着物除去装置の開発~噴射による人工歯石除去と歯の表面性状に関する検討~

    佐藤由加, 飯久保正弘, 西岡貴志, 依田信裕, 楠哲也, 中川敦寛, 冨永悌二

    日本生体医工学会大会プログラム・抄録集(Web)　58th　2019

  19. Fusobacterium nucleatum induces the production of extracellular traps by human mast cells, resulting in inflammatory responses

    多田浩之, 西岡貴志, 松下健二, 尾之上さくら, 川原一芳

    エンドトキシン・自然免疫研究(Web)　22　2019

    ISSN： 2434-1177

  20. パルスジェットを用いたインプラント周囲炎治療システムの開発~人工歯石の除去効果とインプラント表面性状に与える影響~

    佐藤由加, 飯久保正弘, 飯久保正弘, 西岡貴志, 依田信裕, 依田信裕, 中川敦寛

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)　151st　2019

  21. Fusobacterium nucleatum感染によるマスト細胞からの細胞外トラップ産生と炎症誘導

    多田浩之, 西岡貴志, 松下健二, 菅原俊二

    第61回歯科基礎医学会学術大会　2019　2019

    ISSN： 2187-9109

  22. 災害歯科医療学実地研修会の参加者に対するアンケート調査

    小林洋子, 西岡貴志, 冨士岳志, 齋藤恵一, 洪光, 江草宏, 佐々木啓一

    第38回日本歯科医学教育学会総会および学術大会　38th　2019

  23. 口腔癌頸部リンパ節転移の節外進展診断における3テスラblack-blood MR imaging法の有用性

    阪本真弥, 小嶋郁穂, 飯久保正弘, 西岡貴志, 笹野高嗣, 伊藤康一, 青木孝文, 小川武則, 森士朗, 小玉哲也

    歯科放射線　58　2018

    ISSN： 0389-9705

  24. 歯周病原細菌によるヒトマスト細胞からの細胞外トラップ産生と炎症誘導

    多田浩之, 西岡貴志, 松下健二

    第24回エンドトキシン・自然免疫研究会　24th　2018

  25. P.gingivalis感染によりマスト細胞から産生されたIL‐31はclaudin‐1発現抑制を介して歯肉上皮バリア破綻を誘導する

    多田浩之, 沼崎研人, 西岡貴志, 松下健二, 菅原俊二

    Journal of Oral Biosciences Supplement (Web)　2018　ROMBUNNO.O3‐13 (WEB ONLY)　2018

    ISSN： 2187-9109

  26. Porphyromonas gingivalisによるマスト細胞由来interleukin‐31を介した歯肉上皮細胞のclaudin‐1ダウンレギュレーション作用

    多田浩之, 西岡貴志, 根本英二, 松下健二

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)　149th　141 (WEB ONLY)　2018

  27. パルスジェットを用いた新たな歯周炎治療法の開発~人工歯石の除去効果に関する検討~

    佐藤由加, 飯久保正弘, 西岡貴志, 中川敦寛

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)　149th　157 (WEB ONLY)　2018

  28. 歯周病におけるneutrophil extracellular traps産生と血管内皮細胞の炎症誘導

    多田浩之, 西岡貴志, 松下健二, 尾之上さくら, 川原一芳

    第23回日本エンドトキシン・自然免疫研究会　2017/12

  29. 歯科用ＣＴアーチファクト除去ソフトが垂直歯根破折の診断精度に与える影響

    飯久保正弘, 泉田一賢, 熊坂 晃, 西岡貴志, 小嶋郁穂, 笹野高嗣

    日本歯科放射線学会第37回関西・九州合同地方会　2017/12

  30. Fracture-like artifacts of gutta-percha cones on CBCT images influenced by the voxel size and FOV

    Iikubo M, Nishioka T, Kobayashi K, Sasano T

    International Congress of Dento-Maxillo-Facial Radiology　2017/04

  31. The 2017 Japan-NIH Joint Symposium

    Hiroyuki Tada, Takashi Nishioka

    The 2017 Japan-NIH Joint Symposium　2017/02

  32. 歯周病関連細菌による好中球からのneutrophil extracellular traps産生を介した炎症反応の誘導

    多田浩之, 沼崎研人, 西岡貴志, 松下健二, 高田春比古

    エンドトキシン・自然免疫研究(Web)　20　2017

    ISSN： 2434-1177

  33. Comparison of intraoral radiography and CBCT for detection of various horizontal angle root fractures

    Iikubo M, Kamio T, Nishioka T, Igarashi C, Ariji E, Sasano T

    The 11th Asia Congress of Oral and Maxillo-Facial Radiology　2016/11

  34. Nicotine exposure through the nicotinic acetylcholine receptors α7 induces the proliferation of oral cancer cell

    Nishioka T, Tada H, Sasano T

    The 6th interface symposium for interface oral health science　2016/01

  35. Porphyromonas gingivalis-induced production of IL-31 by human mast cells resulting in dysfunction of gingival epithelial barrier

    Hiroyuki Tada, Aya Takase, Takashi Nishioka, Kenji Matsushita, Haruhiko Takada

    The 6th interface symposium for interface oral health science　2016/01

  36. 歯周病関連細菌による好中球のneutrophil extracellular traps産生を介した炎症反応の誘導

    多田浩之, 沼崎研人, 西岡貴志, 松下健二, 高田春比古

    日本エンドトキシン・自然免疫研究会プログラム・抄録集　22nd　29　2016

  37. Nicotine exposure increases the resistance of lung and breast cancer cells to cisplatin through enhancing Bcl-2 stability and promotes activation EGFR signal in oral cancer cells

    Nishioka T, Luo LY, Shen L, He H, Mariyannis A, Sasano T, Chen CY

    19th World Congress on Advances in Oncology and 17th International Symposium on Molecular Medicine 2014　2014/10

  38. 歯科用ＣＴにおける根管充填材のアーチファクトに関する検討―第2報 撮影モードおよび位置付けによる影響―

    飯久保正弘, 小佐野貴識, 小林 馨, 勝又明敏, 佐野 司, 有地榮一朗, 西岡貴志, 小嶋郁穂, 阪本真弥, 笹野高嗣

    第55回NPO法人日本歯科放射線学会　2014/06

  39. Nicotine exposure increases the resistance of cancer cells to cisplatin through enhancing Bcl-2 stability

    Nishioka T, Luo LY, Shen L, He H, Mariyannis A, Chen CY

    Thirteenth Scientific Symposium of the Flight Attendant Medical Research Institute　2014/05

  40. Nicotine exposure increases the resistance of cancer cells

    Takashi Nishioka, Ling-Yu Luo, Ling Shen, Huan He, Alexandros Mariyannis, Takashi Sasano, Changyan Chen

    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE　34　S126-S126　2014

    ISSN： 1107-3756

    eISSN： 1791-244X

  41. インプラント術前ＣＴ画像による下顎骨断面形態の検討

    古内 壽, 小嶋郁穂, 酒井 梓, 金田直人, 西岡貴志, 菅原由美子, 佐藤しづ子, 飯久保正弘, 庄司憲明, 阪本真弥, 笹野高嗣

    第54回NPO法人日本歯科放射線学会　2013/06

  42. 歯科放射線学に対する歯学部学生の意識調査

    田代瑞行, 内沼裕輔, 宮本祥行, 飯久保正弘, 酒井 梓, 西岡貴志, 小嶋郁穂, 菅原由美子, 佐藤しづ子, 古内 壽, 庄司憲明, 阪本真弥, 笹野高嗣

    第54回NPO法人日本歯科放射線学会　2013/06

  43. CT画像にみられる根管充填剤による歯根破折様アーチファクトについて

    飯久保正弘, 西岡貴志, 小林馨, 有地榮一朗, 笹野高嗣

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)　138th　2013/06

  44. 東北大学歯学部・歯学研究科における新機軸の教育研究の展開

    佐々木啓一, 竹内裕尚, 天雲太一, 洪光, 真柳弦, 西岡貴志, 鈴木治, 高橋信博, 小坂健

    みちのく歯学会雑誌　44　(1-2)　2013

    ISSN： 0385-0099

  45. Porphyromonas gingivalis-induced alveolar bone loss in interleukin-18 transgenic mice

    Noriaki Shoji, Kotaro Yoshinaka, Takashi Nishioka, Yumiko Sugawara, Shunji Sugawara, Takashi Sasano

    INTERFACE ORAL HEALTH SCIENCE 2009　220-+　2010

    DOI： 10.1007/978-4-431-99644-6_54 　

  46. The Interleukin-18 transgenic mice are highly susceptible to Porphyromonas gingivalis-induced alveolar bone loss

    Shoji N, Yoshinaka K, Nishioka T, Sugawara Y, Sugawara S, Sasano T

    Tohoku-Forsyth Symposium　2009/03

  47. Analysis of salivary gland disorder and autoantibody by IL-18 overexpression in mice

    Sato K, Kuroishi T, Tanaka Y, Nishioka T, Hoshino T, Sugawara Y, Sugawara S

    The 38th Annual Meeting of the Japanese Society for Immunology　2008/12

  48. Keratin 5 (K5) /IL-18 トランスジェニックマウスにおけるシェーグレン症候群類似唾液腺障害の発現とその解析

    西岡貴志, 黒石智誠, 菅原由美子, 田中志典, 笹野高嗣, 遠藤康男, 菅原俊二

    第50回歯科基礎医学会学術大会・総会　2008/09

  49. Impairment of salivary grands in keratin 5 /IL-18 transgenic mice

    Nishioka T, Kuroishi T, Sugawara Y, Tanaka Y, Sasano T, Endo Y, Sugawara S

    86th General Session & Exhibition of the IADR　2008/07

  50. Induction of serum IL-18 from keratinocytes/epithelial cells in mice treated with Propionibacterium acnes and LPS

    Nishioka T, Kuroishi T, Yu Z, Sugawara Y, Endo Y, Sugawara S

    2006/12

  51. 頸部リンパ節に多発した胚中心進展性異形成の一例

    飯久保正弘, 古内 寿, 阪本真弥, 菅原由美子, 西岡貴志, 小嶋郁穂, 示野陽一, 庄司憲明, 笹野高嗣

    第11回日本歯科放射線学会臨床画像大会　2006/11

  52. Induction of serum IL-18 from keratinocytes/epithelial cells through neutrophil serine protease-mediated protease-activated receptor 2 (PAR2) activation.

    Nishioka T, Ikawa K, Yu Z, Sugawara Y, Sasano T, Endo Y, Sugawara S

    84th General Session & Exhibition of the IADR (oral presentation)　2006/06

  53. Involvement of neutrophils and protease-activated receptor-2 (PAR-2) in the induction of IL-18 from mucosal cells in mice

    Nishioka T, Ikawa K, Yu Z, Sugawara Y, Kawagoe J, Takizawa T, Primo V, Nikolic B, Sasano T, Shimauchi H, Takada H, Endo Y, Sugawara S

    12th International Congress of Mucosal Immunology　2005/06

  54. In vivoにおけるprotease-activate receptor-2 (PAR-2) を介するIL-18産生制御

    伊川桂次, 西岡貴志, 菅原由美子, 笹野高嗣, 島内英俊, 高田春比古, 遠藤康男, 菅原俊二

    第34回日本免疫学会　2004/12

Show all ︎Show first 5

Books and Other Publications 1

  1. 歯周病におけるneutrophil extracellular traps産生と血管内皮細胞の炎症誘導

    多田浩之, 西岡貴志, 松下健二, 尾之上さくら, 川原一芳

    エンドトキシン・自然免疫研究21, 66~69, 医学図書出版株式会社　2018/11

Research Projects 23

  1. ニコチンによる口腔内好中球NETsの質的異常がもたらす口腔がん発生・増悪機構の解明

    西岡 貴志

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2023/04 - 2027/03

  2. 「うま味」感受性低下の原因解明を基軸とした高齢者健康維持の治療基盤構築

    庄司 憲明, 西岡 貴志, 佐藤 しづ子, 駒井 三千夫, 坪井 明人

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2020/04/01 - 2023/03/31

    More details Close

    我が国では超高齢化や食生活の変化などを背景に味覚障害を有する高齢者が急増している。味覚障害は単なる感覚障害に留まらず、食欲不振から体重減少、さらに体調不良に直結することから社会問題になっている。我々は、他の基本４味（甘、酸、塩、苦）の感受性は正常でありながら、「うま味」の感受性のみが低下するため極度の食欲不振に陥る高齢者を数多く経験し報告している。 2020-2021年度は、味覚障害を主訴として東北大学病院を受診した患者を対象として、以下①～③のデータ収集を行った。 ①医療面接―患者背景、年齢、性別、原疾患、既往歴、QOL-ACDによるQOL評価、アンケート調査による味覚・食事の変化、生活の変化、②味覚検査：a)濾紙ディスク法（酸味、苦味、塩味、甘味）テーストディスク; 三和化学研究所、濾紙ディスク法（うま味）、本研究グループ開発、b)電気味覚検査、③唾液検査：a)総唾液分泌量の測定、b)小唾液腺の唾液分泌量測定に関する

  3. NETsを起点とした口腔がん発生機序の解明～口腔細菌とニコチンのクロストーク～

    西岡 貴志

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04/01 - 2023/03/31

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    好中球の新たな機能として、好中球細胞外トラップ Neutrophil extracellular traps (NETs)が注目されている。近年、NETsは、好中球に細菌感染のみならずニコチンの刺激が加わることで誘導されること、NETs産生を起点としてがん細胞の増殖や転移が促進されることなどが明らかとなった。 本研究の目的は、細菌感染やニコチンによる口腔粘膜上皮バリア破綻、NETsによる炎症誘導を検証し、NETsを起点とした口腔がん発生のメカニズムを明らかにすることである。 本年度は、ヒト前骨髄球性細胞株（HL-60細胞）を使用し、ヒストンH3のシトルリン化の解析を行った。HL-60細胞をレチノイン酸で5日間培養し、好中球に分化させた後、ニコチン処理により刺激を加えて反応を確認した。ニコチンの刺激時間および刺激濃度については様々な組み合わせを検討し、ウェスタンブロット法にて解析した。NETs形成の特徴であるヒストンH3のシトルリン化は、低濃度、短時間刺激群では反応がみられなかったが、高濃度、長時間刺激群ではコントロール（無刺激）群と比べ発現上昇が認められた。今回、ニコチン単独刺激でもヒストンH3のシトルリン化が認められたが、さらに他の刺激（細菌など）と組み合わせることでさらなる発現上昇や発現のピークの変化、ニコチン低濃度刺激での発現など相乗的な増強反応が予想されるところであり、こちらについては現在解析を進めているところである。

  4. 苦味受容体と解毒酵素の多型解析と医療ビックデ－タによる発がんリスク評価法の開発

    庄司 憲明, 坪井 明人, 笹野 高嗣, 西岡 貴志, 佐藤 しづ子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的研究(萌芽)

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2019/06/28 - 2022/03/31

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    近年、ブロッコリーなどのアブラナ科植物の摂取量が多い集団では、肺がんや乳がんの発症率が低いという疫学調査が報告され(Cancer Epidemiol. Biomarks Prev.18, 184-195, 2009)、アブラナ科植物に含まれる苦味物質(isothiocyanate類)の発がん抑制作用が明らかになった。そこで、本研究では消化器がんを有する患者を対象とし、官能検査やアンケート調査、苦味の嗜好に関連する苦味受容体遺伝子や肝臓解毒酵素遺伝子の一塩基多型（SNPs）解析を行い、苦味感受性と肝臓解毒酵素に関する発がんリスクを明らかにすることを目的とした。 2021年度は東北メディカル・メガバンク機構（Tohoku Medical Megabank Organization (ToMMo)）のリファレンスパネル2000人規模のデータを利用して、苦味受容体の遺伝子多型と、アルコール飲料嗜好性等のアンケート調査データに記載された食習慣との関連性について解析した。

  5. 歯周病原細菌による好中球細胞外トラップ誘導を基軸とした歯肉上皮バリア破綻の解明

    多田 浩之, 西岡 貴志, 松下 健二

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2019/04/01 - 2022/03/31

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    慢性歯周炎において、歯周病原細菌により誘導される慢性炎症は歯周組織を破壊する主体となる。よって、歯周病原細菌の感染に対する宿主細胞の慢性炎症反応の機序を解明することは、歯周病の予防・治療法の開発基盤として重要な意義を示す。本研究は、歯周病原細菌の感染による好中球から産生される好中球細胞外トラップ(neutrophil extracellular traps; NETs)の誘導メカニズムならびにNETsによる口腔粘膜バリアが破綻する機序を解明することを目的とする。 昨年度の研究実績から、ヒト好中球は歯周病原細菌Fusobacterium nucleatum感染によりNETsを産生することを見出した。細胞外トラップは好中球のみならず、多様な免疫細胞から産生される。今年度は、マスト細胞に着目し、F. nucleatum感染によるヒトマスト細胞からの細胞外トラップの産生について解析した。その結果、F. nucleatum感染によりマスト細胞は細胞外トラップDNAを放出することが観察され、このDNA構造物はシトルリン化されたヒストンH3を含むことから、マスト細胞外トラップ(mast cell extracellular traps; MCETs)であることを明らかにした。次にF. nucleatum感染によりマスト細胞から放出されたMCETsに発現する分子群について、サイトカインアレイ法で解析した結果、F. nucleatum感染によりマスト細胞から放出されたMCETsには炎症の惹起を担うマクロファージ遊走阻止因子(macrophage migration inhibitory factor, MIF)が高レベルで発現することを見出した。

  6. Study on taste disorders and its treatment strategies: A comparative study between Japan and Thailand

    SHOJI NORIAKI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

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    Decreased salivary secretion is one of the main causes of taste disorder. It has been reported that improving salivary secretion function using umami stimulation may be a safe and reliable treatment for taste disorder. In the present study, we examined the relationship between the recognition threshold of umami and sour taste and the flow rate both of total and of minor saliva by gustatory-salivary reflex in Thai subjects with low sensitivity to umami in order to compare the data with those of Japanese subjects with high sensitivity to umami. The results suggested that umami stimulation was effective only for elders with hyposalivation, while acid stimulation was effective for all participants. These results suggest that acid stimulation is more effective than umami stimulation in taste disordered subjects with low sensitivity to umami threshold.

  7. Porphyromonas gingivalis induces the production of IL-31 by human mast cells, resulting in dysfunction of the gingival epithelial barrier

    Tada Hiroyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/04/01 - 2020/03/31

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    Interleukin (IL)-31 participates in the pathogenesis of chronic inflammatory diseases. We investigated the contribution of mast cells to the induction of IL-31 production following infection with the periodontal pathogen, Porphyromonas gingivalis. We found that oral infection with P. gingivalis increased IL-31 expression in the gingival tissues of mice. The P. gingivalis-induced IL-31 production by human mast cells was dependent on the P. gingivalis lysine-specific protease gingipain-K. Notably, the P. gingivalis-induced IL-31 production by mast cells led to the downregulation of claudin-1, a tight junction molecule, in gingival epithelial cells, resulting in an IL-31-dependent increase in the paracellular permeability of the gingival epithelial barrier. These findings suggest that IL-31 produced by mast cells in response to P. gingivalis infection causes gingival epithelial barrier dysfunction, which may contribute to the chronic inflammation observed in periodontitis.

  8. 味覚障害の病因解明と治療戦略の構築 ー日本ータイ二国間比較研究ー Competitive

    庄司 憲明

    Offer Organization: 文部科学省: 科学研究費 基盤研究(B)

    2017/04 - 2020/03

  9. New theory for chronic pain control by saliva-derived antinociceptive modulator.

    Shoji Noriaki, TSUCHIYA masahiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2019/03/31

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    Recently, patients with chronic pain have been increasing and recognized as social issues. This study was designed to determine the effect of sialorphin, which was saliva-derived antinociceptive modulator, on mechanical allodynia using the psychosocial stress mice model. Consequently, immunohistochemistry analysis could suggest the localization of sialorphin receptor in the schwann cells and/or satellite cells, although sialorphin had no obvious inhibitory effect on mechanical allodynia. These results suggested that signal induced by sialorphin could inhibit enhancement of trigeminal excitation.

  10. Elucidation of oral cancer cell proliferation mechanism by nicotine and enlightenment of smoking cessation based on scientific evidence.

    Nishioka Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2015/04/01 - 2019/03/31

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    In this study, I focused on the nicotine, which is one of the main components of tobacco. Although, the nicotine is present in high concentrations in the bloodstream of smokers, its effect on carcinogenesis is not clear yet. The nicotine was used to examine the effect the cell growth, division and differentiation of the oral cancer cells. The oral cancer cells were stimulated by nicotine, cells were analyzed using molecular biological techniques such as Western blotting. The results showed that the nicotine induced the phosphorylation of epidermal growth factor receptor (EGFR) through the nicotinic acetylcholine receptor (nAChR), and its downstream molecular promoter activation proteins were activated. These results suggest that the nicotine was activated the cell proliferation signals and might be potential to promote the proliferation and division in oral cancer cells.

  11. 歯周病原細菌によるマスト細胞のIL-31を基軸とした歯肉上皮バリア破綻機序の解明 Competitive

    多田 浩之

    Offer Organization: 文部科学省: 科学研究費 基盤研究(C)

    2016/04 - 2019/03

  12. Therapeutic innovation in oral mucosal leasions with erosion and ulcer by analyzing antigen presenting ability and autophagy

    Sugawara Yumiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2018/03/31

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    Retrospective study was performed at 12 facilities on efficacy and safety of Cepharanthin (CEP) long-term use for the patient with oral lichen planus (OLP). The degree of the severity classified to, before and after 4weeks and 6 months and at the end point of the CEP use was assessed. The mean dosage period of the CEP use was 415 days, and the daily average dosage was 11.6mg. The high improvement rate was accepted in almost all clinical types and the location of the lesions by CEP use. Long-term use showed the higher efficacy for improving OLP. On the other hand, the dosage of CEP showed no significant difference for the improvement of OLP. High improvement rate was recognized even in the case without steroid use. Finally, eighty percent of the OLP cases showed significant efficacy, in addition, there was no significant adverse events due to the side effects. Thus, CEP use is considered to have significant efficacy for the treatment of OLP without side effects.

  13. 唾液由来鎮痛物質による新たな慢性疼痛制御理論の確立 Competitive

    庄司 憲明

    Offer Organization: 文部科学省: 科学研究費 挑戦的萌芽研究

    2015/04 - 2018/03

  14. ニコチンによる口腔がん細胞増殖メカニズムの解明と科学的根拠に基づく禁煙の啓蒙 Competitive

    西岡 貴志

    Offer Organization: 文部科学省: 科学研究費 若手研究(B)

    2015/04 - 2018/03

  15. 口腔粘膜のびらん・潰瘍病変における抗原提示能とオートファジー解析による治療創生 Competitive

    菅原 由美子

    Offer Organization: 文部科学省: 科学研究費 基盤研究(C)

    2014/04 - 2018/03

  16. Comparative study on taste sensitivity between Japan and Thailand

    Shoji Noriaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2013/04/01 - 2017/03/31

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    Taste perception is influenced by several factors. However, it was unclear how food culture affects taste perception. In this study, we compared recognition taste thresholds between populations with different food culture, i.e. Thai and Japanese Adults. A matched case-control study was conducted in 168 healthy older adults (84 for each country). Recognition thresholds of sweet, salty, sour, bitter and umami were measured using Filter Paper Disc by stimulation at anterior and posterior tongue area. Spicy preference was measured by calibrated questionnaires. Interestingly, Thai older adults had much higher recognition thresholds of all tastes than those of Japanese (p<0.0001). Further, Thai adults had stronger preference on spicy food (p<0.0001). Our findings suggest that population from culture with stronger preference on spicy food such as Thai had higher recognition thresholds of all tastes than population with milder preference on spicy food like Japanese.

  17. Analysis of the human salivary-gland apoptotic pass way for Sjogren's syndrome.

    MUROI AZUSA

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2013/04/01 - 2017/03/31

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    Sjogren’s syndrome is an inflammatory autoimmune disease, predominantly characterized by lymphocytic infiltration of the exocrine glands. In this study, to establish prophylactic and therapeutic strategies for this disorder, we examined the association between interleukin 18 (IL-18) and the apoptosis pathway in the salivary gland tissues of humans. The HSG cell line expresses caspase-1, which processes the IL-18 precursor protein into its mature form. However, ASC and NLRP3, which together with caspase-1 constitute the imflammasome complex, were not detected in these cells. Furthermore, we detected no change in the activity of intracellular caspase-1, even when the cells were stimulated with ATP and nigericin to induce intracellular K+, a factor known to trigger the imflammasome. Therefore, we infer that the activation of IL-18 in this cell line occurs without the activation of the imflammasome.

  18. 日本－タイの二国間比較による味覚障害の病因解明と治療戦略の構築 Competitive

    庄司 憲明

    Offer Organization: 文部科学省: 科学研究費 基盤研究(B)

    2013/04 - 2017/03

  19. シェーグレン症候群の克服に向けたヒト唾液腺アポトーシスパスウェイの解析 Competitive

    室井 梓

    Offer Organization: 文部科学省: 科学研究費 基盤研究(C)

    2013/04 - 2017/03

  20. Elucidation of the molecular basis related to the nicotinic acetylcholine receptor involved in the development of oral cancer

    NISHIOKA TAKASHI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    The tobacco is one of the major risk factor for oral cancer. Nicotine is a main component of tobacco, but has not been demonstrated to affect directly carcinogenesis yet. In the present study, nicotine promotes phosphorylation of Epidermal Growth Factor Receptor (EGFR) signal, which pathways associated with mitogenic for cells, also the involvement of nicotinic acetylcholine receptor α7 was suggested.

  21. 口腔がんの発症に係るニコチン性アセチルコリンレセプターに関する分子基盤の解明 Competitive

    西岡 貴志

    Offer Organization: 文部科学省: 科学研究費 若手研究(B)

    2013/04 - 2016/03

  22. Impairment of masticatory ability would induce the metabolic syndrome.

    TSUCHIYA Masahiro, SASAKI Keiichi, KUROISHI Toshinobu, NISHIOKA Takashi, ENDO Yasuo, YAOITA Fukie

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    The hyperglycemia associated with long-term powdered-food feeding may lead to certain systemic illness signs, such as elevations of blood glucose and hypertension in mice. Mastication is very important for the maintenance of systemic health, via reduction in the levels of blood glucose and/or stress hormones . Chewing dysfunction was reported as a risk factor for metabolic syndrome. Our results suggest that the dietary habits of patients are as important as the nutritional dietary information that they receive. Diet therapy is critically important in the management of diabetes. Meanwhile, chewing ability is often neglected in the care of diabetic patients. Considering that people who are losing their teeth tends to prefer a soft diet, patients with chewing dysfunctions such as edentulous patients could not properly undergo diet therapy. With decreased chewing function, dental care, including implants, should be an effective choice compared with simple medication management.

  23. 咀嚼の崩壊は代謝性疾患の発症基盤－Network Medicineの視点から－ Competitive

    土谷 昌広

    Offer Organization: 文部科学省: 科学研究費 挑戦的萌芽研究

    2013/04 - 2015/03

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