Details of the Researcher

PHOTO

Hideaki Takeuchi
Section
Graduate School of Life Sciences
Job title
Professor
Degree

  • 博士（薬学）（東京大学）

  • 修士（薬学）（東京大学）

e-Rad No.
00376534
Profile

To clarify both molecular/neural basis and brain information processing underlying social interactions in vertebrates, we have focused on Medaka fish and established novel behavior systems to assess social interactions (schooling behavior, individual recognition, group-escape and group-learning). Using these systems we are planning to identify internal factors (genes, neural networks, and brain regions) essential for vertebrate social interactions using transgenic medaka systems. On the other hand, to estimate behavior rules underlying social interactions, we developed a hypothesis-independent data mining, which could explain actual fish movement. Our eventual purpose is to how the internal factors (genes, neural networks, and brain regions) influences animal behaviors which can serve as the basis for the emergence of sociality.
Furthermore I have investigated the molecular/neural basis underlying invertebrate social behavior (honeybee) and compare neural/ social mechanisms between the vertebrate and invertebrate.

Research History 19

  • 2022/04 - Present
    Utsunomiya University　Center for Optical Research and Education

  • 2019/11 - Present
    Tohoku University　Graduate School of Life Sciences　Professor

  • 2019/11 - 2021/03
    Okayama University　Specially-appointed Professor

  • 2015/04 - 2019/10
    Okayama University　Graduate School of Natural Science and Technology　Associate Professor

  • 2006/04 - 2015/03
    The University of Tokyo　Graduate School of Science, Department of Biological Sciences

  • 2004/04 - 2015/03
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo　Assistant Professor

  • 2004/04 - 2006/03
    The University of Tokyo　Graduate School of Science, Department of Biological Sciences

  • 2003/04 - 2004/03
    The University of Tokyo　Graduate School of Science

  • 1999/04 - 2004/03
    Postdoctral fellow (Supervisor: Prof. Takeo Kubo)

  • 2000/05 - 2003/03
    生物系特定産業技術研究推進機構　派遣研究員

  • 2000/04 - 2000/05
    日本学術振興会未来開拓事業　リサーチアソシエイト

  • 1999/04 - 2000/03
    日本学術振興会平成10年度採用特別研究員　PD

  • 1998/04 - 1999/03
    日本学術振興会平成10年度採用特別研究員　DC2

  • 1996/04 - 1999/03
    Graduate School of Pharmaceutical Sciences, The University of Tokyo　Doctor (Pharmacy)

  • 1996/04 - 1999/03
    The University of Tokyo　Graduate School of Pharmaceutical Science Doctoral Program, Department of Pharmaceutical Biology

  • 1994/04 - 1996/03
    Graduate School of Pharmaceutical Sciences, The University of Tokyo　Master (Pharmacy)

  • 1994/04 - 1996/03
    The University of Tokyo　Graduate School of Pharmaceutical Sciences

  • 1992/04 - 1994/03
    Faculty of Pharmaceutical Sciences, The University of Tokyo　Bachelor (Pharmacy)

  • 1992/04 - 1994/03
    The University of Tokyo　Faculty of Pharmaceutical Sciences

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Professional Memberships 3

  • Japanese Society for Behavioral Neuroendocrinology

  • The Japan Neuroscience Society

  • 日本動物学会

Research Interests 6

  • 包括脳ネットワーク

  • 脳

  • 神経

  • 行動生物学

  • 分子生物学

  • 社会性行動

Research Areas 1

  • Life sciences / Neuroscience - general /

Papers 90

  1. Establishment of a transgenic strain for the whole brain calcium imaging in larval medaka fish ( Oryzias latipes )

    Takahide Seki, Kazuhiro Miyanari, Asuka Shiraishi, Sachiko Tsuda, Satoshi Ansai, Hideaki Takeuchi

    BioRxiv　2025/04/10

    DOI： 10.1101/2025.04.09.647916 　

  2. The Medaka Approach to Evolutionary Social Neuroscience International-journal Invited Peer-reviewed

    Satoshi ANSAI, Towako HIRAKI-KAJIYAMA, Ryutaro UEDA, Takahide SEKI, Saori YOKOI, Takafumi KATSUMURA, Hideaki TAKEUCHI

    Neuroscience Research　2024/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.neures.2024.10.005 　

    ISSN： 0168-0102

  3. Unilateral Chasing and Courtship-Like Display as Dominance Signals for Hierarchy Maintenance in Male Medaka Fish

    Yuji Mitsutsuji, Mayuko Suzuki, Ryo Umeda, Satoshi Ansai, Fumitaka Ueoka, Zou Jingyi, Anna Yamaguchi, Towako Hiraki-Kajiyama, Tetsuro Takeuchi, Maho Ogoshi, Takafumi Katsumura, Saori Yokoi, Hideaki Takeuchi

    Research Square　2024/09

    DOI： 10.21203/rs.3.rs-4837620/v1 　

  4. Rapid body colouration changes in Oryzias celebensis as a social signal influenced by environmental background. International-journal

    Ryutaro Ueda, Satoshi Ansai, Hideaki Takeuchi

    Biology letters　20　(7)　20240159-20240159　2024/07

    DOI： 10.1098/rsbl.2024.0159 　

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    Rapid body colouration changes in some animals, such as chameleons and octopuses, serve dual functions: camouflage and intraspecific communication. It has been hypothesized that these colouration changes originally evolved to provide camouflage and subsequently were co-opted as social signals; however, experimental model systems that are suitable for studying such evolutionary processes are limited. Here, we investigated the relationship between rapid colouration changes of the blackened markings and aggressive behaviours in male Oryzias celebensis, an Indonesian medaka fish, under triadic relationships (two males and one female) or three males conditions with two different environmental backgrounds. In an algae-covered tank, mimicking the common laboratory rearing conditions, males with blackened markings exhibited more frequent attacks towards different conspecific individuals compared with non-blackened males and females. The blackened males were seldom attacked by non-blackened males and females. By contrast, neither aggressive behaviours nor black colouration changes were observed in the transparent background condition with a brighter environment. These indicated that the blackened markings in O. celebensis serve as a social signal depending on the environmental backgrounds. Considering that such colouration changes for camouflage are widely conserved among teleost fishes, the traits are likely to be co-opted for displaying social signals in O. celebensis.

  5. Unique spatially and temporary-regulated/sex-specific expression of a long ncRNA, Nb-1, suggesting its pleiotropic functions associated with honey bee lifecycle. International-journal

    Hiroto Tadano, Hiroki Kohno, Hideaki Takeuchi, Takeo Kubo

    Scientific reports　14　(1)　8701-8701　2024/04/15

    DOI： 10.1038/s41598-024-59494-6 　

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    Honey bees are social insects, and each colony member has unique morphological and physiological traits associated with their social tasks. Previously, we identified a long non-coding RNA from honey bees, termed Nb-1, whose expression in the brain decreases associated with the age-polyethism of workers and is detected in some neurosecretory cells and octopaminergic neurons, suggesting its role in the regulation of worker labor transition. Herein, we investigated its spatially and temporary-regulated/sex-specific expression. Nb-1 was expressed as an abundant maternal RNA during oogenesis and embryogenesis in both sexes. In addition, Nb-1 was expressed preferentially in the proliferating neuroblasts of the mushroom bodies (a higher-order center of the insect brain) in the pupal brains, suggesting its role in embryogenesis and mushroom body development. On the contrary, Nb-1 was expressed in a drone-specific manner in the pupal and adult retina, suggesting its role in the drone visual development and/or sense. Subcellular localization of Nb-1 in the brain during development differed depending on the cell type. Considering that Nb-1 is conserved only in Apidae, our findings suggest that Nb-1 potentially has pleiotropic functions in the expression of multiple developmental, behavioral, and physiological traits, which are closely associated with the honey bee lifecycle.

  6. Epigenetically distinct synaptic architecture in clonal compartments in the teleostean dorsal pallium. International-journal

    Yasuko Isoe, Ryohei Nakamura, Shigenori Nonaka, Yasuhiro Kamei, Teruhiro Okuyama, Naoyuki Yamamoto, Hideaki Takeuchi#, Hiroyuki Takeda#, #These authors have equally supervised the work

    eLife　12　2023/07/25

    DOI： 10.7554/eLife.85093 　

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    The dorsal telencephalon (i.e. the pallium) exhibits high anatomical diversity across vertebrate classes. The non-mammalian dorsal pallium accommodates various compartmentalized structures among species. The developmental, functional, and evolutional diversity of the dorsal pallium remain unillustrated. Here, we analyzed the structure and epigenetic landscapes of cell lineages in the telencephalon of medaka fish (Oryzias latipes) that possesses a clearly delineated dorsal pallium (Dd2). We found that pallial anatomical regions, including Dd2, are formed by mutually exclusive clonal units, and that each pallium compartment exhibits a distinct epigenetic landscape. In particular, Dd2 possesses a unique open chromatin pattern that preferentially targets synaptic genes. Indeed, Dd2 shows a high density of synapses. Finally, we identified several transcription factors as candidate regulators. Taken together, we suggest that cell lineages are the basic components for the functional regionalization in the pallial anatomical compartments and that their changes have been the driving force for evolutionary diversity.

  7. Optogenetic control of medaka behavior with channelrhodopsin.

    Takahide Seki, Hideaki Takeuchi, Satoshi Ansai

    Development, growth & differentiation　2023/06/24

    DOI： 10.1111/dgd.12872 　

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    Optogenetics enables the manipulation of neural activity with high spatiotemporal resolution in genetically defined neurons. The method is widely used in various model animals in the neuroscience and physiology fields. Channelrhodopsins are robust tools for optogenetic manipulation, but they have not yet been used for studies in medaka. In the present study, we used the CRISPR/Cas9-mediated knock-in approach to establish a transgenic medaka strain expressing the Chloromonas oogama channelrhodopsin (CoChR) in the ISL LIM homeobox 1 (isl1) locus. We demonstrated that light stimuli elicited specific behavioral responses such as bending or turning locomotion in the embryos and pectoral fin movements in the larvae and adults. The response probabilities and intensities of these movements could be controlled by adjusting the intensity, duration, or wavelength of each light stimulus. Furthermore, we demonstrated that the pectoral fin movements in the adult stage could be elicited using a laser pointer to irradiate region including the caudal hind brain and the rostral spinal cord. Our results indicate that CoChR allows for manipulation of medaka behaviors by activating targeted neurons, which will further our understanding of the detailed neural mechanisms of motor control or social behaviors in medaka. This article is protected by copyright. All rights reserved.

  8. Spatio-temporal control of targeted gene expression in combination with CRISPR/Cas and Tet-On systems in Medaka. International-journal

    Daichi Kayo, Sayaka Kimura, Touko Yamazaki, Kiyoshi Naruse, Hideaki Takeuchi, Satoshi Ansai

    Genesis (New York, N.Y. : 2000)　e23519　2023/05/25

    DOI： 10.1002/dvg.23519 　

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    Spatial and temporal control of transgene expression is a powerful approach to understand gene functions in specific cells and tissues. The Tet-On system is a robust tool for controlling transgene expression spatially and temporally; however, few studies have examined whether this system can be applied to postembryonic stages of Medaka (Oryzias latipes) or other fishes. Here, we first improved a basal promoter sequence on the donor vector for a nonhomologous end joining (NHEJ)-based knock-in (KI) system. Next, using transgenic Medaka for establishing the Tet-On system by KI, we demonstrated that doxycycline administration for four or more days by feeding can be a stable and efficient method to achieve expression of the transduced reporter gene in adult fish. From these analyses, we propose an optimized approach for a spatio-temporal gene-expression system in the adult stage of Medaka and other small fishes.

  9. Mating experiences with the same partner enhanced mating activities of naïve male medaka fish. International-journal

    Masahiro Daimon, Takafumi Katsumura, Hirotaka Sakamoto, Satoshi Ansai, Hideaki Takeuchi

    Scientific reports　12　(1)　19665-19665　2022/11/16

    DOI： 10.1038/s41598-022-23871-w 　

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    Mating experience shapes male mating behavior across species, from insects, fish, and birds, to rodents. Here, we investigated the effect of multiple mating experiences on male mating behavior in "naïve" (defined as sexually inexperienced) male medaka fish. The latency to mate with the same female partner significantly decreased after the second encounter, whereas when the partner was changed, the latency to mate was not decreased. These findings suggest that mating experiences enhanced the mating activity of naïve males for the familiar female, but not for an unfamiliar female. In contrast, the mating experiences of "experienced" (defined as those having mated > 7 times) males with the same partner did not influence their latency to mate. Furthermore, we identified 10 highly and differentially expressed genes in the brains of the naïve males after the mating experience and revealed 3 genes that are required for a functional cascade of the thyroid hormone system. Together, these findings suggest that the mating experience of naïve male medaka fish influences their mating behaviors, with neural changes triggered by thyroid hormone activation in the brain.

  10. Collective Decision-making in Response to Visual Looming Stimuli in Medaka Fish

    Ryohei Nakahata, Hideaki Takeuchi

    BioRxiv　2022/06/16

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2022.06.14.494464 　

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    Various social animals synchronously make behavioral choices among alternatives (i.e., collective decision-making) to efficiently migrate, forage, or escape from predators. Here, we demonstrated that medaka fish, Oryzias latipes, make collective decisions in response to visual looming stimuli (LS), a simulated predator attack. The group-level response to the LS could be classified into 2 patterns: freezing synchronously or not freezing, indicating that medaka selected their response to the LS from 2 alternative choices. Furthermore, the behavioral response to the LS persisted over multiple behavioral trials for 3 days, indicating that each group made a consistent decision to freeze or not to freeze. Finally, we showed that the group response could be predicted by the maximum swimming speed among individuals during the LS presentation. If a group included at least 1 individual that responded to the LS with high-speed movement (>7.4 cm/s), the group tended to freeze synchronously in response to the LS. If no member of the group was moving at high-speed during the LS presentation, the group tended to continue moving after the LS presentation. Our findings suggest that the presence of 1 or more individual medaka with a strong fear response in a group determines the group-level decision of how to respond to a threatening stimulus.

  11. Quantifying social Interactions in medaka fish

    Yokoi S, Isoe Y, Wang MY, Daimon M, Okuyama T, Takeuchi H

    Behavioral Neurogenetics　93-105　2022

    DOI： 10.1007/978-1-0716-2321-3_7 　

    ISSN： 0893-2336

    eISSN： 1940-6045

  12. A modified Tet-ON system minimizing leaky expression for cell-type specific gene induction in medaka fish.

    Osamu Hosoya, Myung Chung, Satoshi Ansai, Hideaki Takeuchi, Mary Miyaji

    Development, growth & differentiation　2021/08/10

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    The Tet-ON system is an important molecular tool for temporally and spatially-controlled inducible gene expression. Here, we developed a Tet-ON system to induce transgene expression specifically in the rod photoreceptors of medaka fish. Our modified reverse tetracycline-controlled transcriptional transactivator (rtTAm) with 5 amino acid substitutions dramatically improved the leakiness of the transgene in medaka fish. We generated a transgenic line carrying a self-reporting vector with the rtTAm gene driven by the Xenopus rhodopsin promoter and a tetracycline response element (TRE) followed by the green fluorescent protein (GFP) gene. We demonstrated that GFP fluorescence was restricted to the rod photoreceptors in the presence of doxycycline in larval fish (9 days post-fertilization). The GFP fluorescence intensity was enhanced with longer durations of doxycycline treatment up to 72 h and in a dose-dependent manner (5-45 μg/ml). These findings demonstrate that the Tet-ON system using rtTAm allows for spatiotemporal control of transgene expression, at least in the rod photoreceptors, in medaka fish.

  13. Sexually dimorphic role of oxytocin in medaka mate choice. International-journal Peer-reviewed

    Saori Yokoi, Kiyoshi Naruse, Yasuhiro Kamei, Satoshi Ansai, Masato Kinoshita, Mari Mito, Shintaro Iwasaki, Shuntaro Inoue, Teruhiro Okuyama, Shinichi Nakagawa, Larry J Young, Hideaki Takeuchi

    Proceedings of the National Academy of Sciences of the United States of America　117　(9)　4802-4808　2020/03/03

    DOI： 10.1073/pnas.1921446117 　

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    Oxytocin is a central neuromodulator required for facilitating mate preferences for familiar individuals in a monogamous rodent (prairie vole), irrespective of sex. While the role of oxytocin in mate choice is only understood in a few monogamous species, its function in nonmonogamous species, comprising the vast majority of vertebrate species, remains unclear. To address this issue, we evaluated the involvement of an oxytocin homolog (isotocin, referred herein as oxt) in mate choice in medaka fish (Oryzias latipes). Female medaka prefer to choose familiar mates, whereas male medaka court indiscriminately, irrespective of familiarity. We generated mutants of the oxt ligand (oxt) and receptor genes (oxtr1 and oxtr2) and revealed that the oxt-oxtr1 signaling pathway was essential for eliciting female mate preference for familiar males. This pathway was also required for unrestricted and indiscriminate mating strategy in males. That is, either oxt or oxtr1 mutation in males decreased the number of courtship displays toward novel females, but not toward familiar females. Further, males with these mutations exhibited enhanced mate-guarding behaviors toward familiar females, but not toward novel females. In addition, RNA-sequencing (seq) analysis revealed that the transcription of genes involved in gamma-amino butyric acid metabolism as well as those encoding ion-transport ATPase are up-regulated in both oxt and oxtr1 mutants only in female medaka, potentially explaining the sex difference of the mutant phenotype. Our findings provide genetic evidence that oxt-oxtr1 signaling plays a role in the mate choice for familiar individuals in a sex-specific manner in medaka fish.

  14. Aerial display opens a new field of biology

    Hirotsugu Yamamoto, Erina Abe, Masaki Yasugi, Eiji Watanabe, Hideaki Takeuchi

    Proceedings of SPIE - The International Society for Optical Engineering　11520　2020

    Publisher: SPIE

    ISSN： 1996-756X 0277-786X

  15. Development of omnidirectional aerial display with aerial imaging by retro-reflection (AIRR) for behavioral biology experiments Peer-reviewed

    Erina Abe, Masaki Yasugi, Hideaki Takeuchi, Eiji Watanabe, Yasuhiro Kamei, Hirotsugu Yamamoto

    Optical Review　26　(1)　221-229　2019/02

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10043-019-00502-w 　

    ISSN： 1340-6000

    eISSN： 1349-9432

  16. Aquatic information display and its applications for behavioral biology experiments

    Hirotsugu Yamamoto, Erina Abe, Masaki Yasugi, Eiji Watanabe, Hideaki Takeuchi

    Proceedings of SPIE - The International Society for Optical Engineering　10997　2019

    Publisher: SPIE

    DOI： 10.1117/12.2521225 　

    ISSN： 1996-756X 0277-786X

  17. Clonal analysis of construction mechanism of the adult telencephalon mediated by post-hatch neurogenesiss in medaka fish Peer-reviewed

    Yasuko Isoe, Ryohei Nakamura, Yasuhiro Kamei, Shigenori Nonaka, Teruhiro Okuyama, Atsushi Shimizu, Takeo Kubo, Hiroyuki Takeda, Hideaki Takeuchi

    Mechanisms of Development　145　S118-S119　2017/07

    DOI： 10.1016/j.mod.2017.04.320 　

    ISSN： 0925-4773

    eISSN： 1872-6356

  18. Individual recognition and the 'face inversion effect' in medaka fish (Oryzias latipes) Peer-reviewed

    Mu-Yun Wang, Hideaki Takeuchi

    ELIFE　6　2017/07

    DOI： 10.7554/eLife.24728 　

    ISSN： 2050-084X

  19. Molecular basis of social competence in medaka fish. Peer-reviewed

    Teruhiro Okuyama, Saori Yokoi, Hideaki Takeuchi

    Development, growth & differentiation　59　(4)　211-218　2017/05

    DOI： 10.1111/dgd.12359 　

    ISSN： 0012-1592

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    Oryzias latipes (Medaka) is an established vertebrate model for studying developmental genetics, genomics, and evolutionary biology. The physiology, embryology, and genetics of this species have been extensively investigated for centuries. Medaka fish recently attracted attention in the field of social neuroscience. This review introduces recent advances in medaka behavioral studies, focusing on female mating preferences and male mate-guarding behaviors. The medaka female has the ability to discriminate male individuals and prefers to mate with socially familiar males (female mating preference). In triadic relationships (two males and one female), the dominant male remains closer to the female and repels the other male (mate-guarding). Interestingly, mate-guarding blocks female social familiarization of the rival male, which can increase the mating success of the dominant male. Importantly, behavioral analyses using a series of medaka mutants revealed critical roles of neuropeptide neuromodulatory systems in regulating their social behaviors. The extra-hypothalamic gonadotropin-releasing hormone system has a central role in activating female mating preference. The arginine-vasotocin system is required for the emergence of mate-guarding behavior.

  20. Omnidirectional aerial display for medaka

    Erina Abe, Sho Onose, Hideaki Takeuchi, Eiji Watanabe, Yasuhiro Kamei, Hirotsugu Yamamoto

    Proceedings of the International Display Workshops　2　932-934　2017

    Publisher: International Display Workshops

    ISSN： 1883-2490

  21. Adrenomedullin 2 and 5 activate the calcitonin receptor-like receptor (clr) - Receptor activity-modifying protein 3 (ramp3) receptor complex in Xenopus tropicalis. International-journal

    Maho Ogoshi, Mikoto Takahashi, Kota Aoyagi, Kazuyoshi Ukena, Sayaka Aizawa, Hideaki Takeuchi, Sumio Takahashi, Sakae Takeuchi

    General and comparative endocrinology　306　113752-113752　2021/05/15

    DOI： 10.1016/j.ygcen.2021.113752 　

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    The adrenomedullin (AM) family is involved in diverse biological functions, including cardiovascular regulation and body fluid homeostasis, in multiple vertebrate lineages. The AM family consists of AM1, AM2, and AM5 in tetrapods, and the receptor for mammalian AMs has been identified as the complex of calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 2 (RAMP2) or RAMP3. However, the receptors for AM in amphibians have not been identified. In this study, we identified the cDNAs encoding calcrl (clr), ramp2, and ramp3 receptor components from the western clawed frog (Xenopus tropicalis). Messenger RNAs of amphibian clr and ramp2 were highly expressed in the heart, whereas that of ramp3 was highly expressed in the whole blood. In HEK293T cells expressing clr-ramp2, cAMP response element luciferase (CRE-Luc) reporter activity was activated by am1. In HEK293T cells expressing clr-ramp3, CRE-Luc reporter activity was increased by the treatment with am2 at the lowest dose, but with am5 and am1 at higher dose. Our results provided new insights into the roles of AM family peptides through CLR-RAMP receptor complexes in the tetrapods.

  22. DNA methylation site loss for plasticity-led novel trait genetic fixation

    Takafumi Katsumura, Suguru Sato, Kana Yamashita, Shoji Oda, Takashi Gakuhari, Shodai Tanaka, Kazuko Fujitani, Toshiyuki Nishimaki, Tadashi Imai, Yasutoshi Yoshiura, Hirohiko Takeshima, Yasuyuki Hashiguchi, Hiroshi Mitani, Motoyuki Ogawa, Hideaki Takeuchi, Hiroki Oota

    2020/07/10

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2020.07.09.194738 　

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    <title>Abstract</title>Many organisms exhibit phenotypic plasticity that changes their traits in response to their environment. Although whether or not this plasticity contributes to adaptive evolution is a fundamental question in evolutionary biology, various studies report that natural populations adapt to rapid environmental changes via plasticity, which leads to novel adaptive traits as “triggers.” Namely, phenotypic plasticity has considered allowing an accumulation of genetic mutations to fix the alternative phenotypes induced by nongenetic perturbations that include gene expression noise or epigenetic modification caused by environmental change. However, because the molecular mechanism of phenotypic plasticity is unknown, verification of the process from phenotypic plasticity to genetic fixation remains insufficient. Here we show that decrease in methylated CpG sites leads to loss of plasticity, which triggers genetic fixation of novel traits, in medaka fish (<italic>Oryzias latipes</italic>). We found that the gut length was correlated with the number of methylated CpG sites upstream of the <italic>Plxnb3</italic> gene, which is involved in the developmental process of nerve axons. The medaka, in which the methylated DNA region is deleted by CRISPR/Cas9, showed a loss of plasticity in gut length and a lower survival rate caused by nonoptimal feeding environments. Moreover, standing variation in the promoter region of another gene, <italic>Ppp3r1</italic>, which is also related to nerve axon development, raised the gene expression and made a longer gut stably in wild medaka groups that lost the gut-length plasticity. Furthermore, our phylogenetic analysis revealed the timing of these evolutionary events, indicating that the loss of phenotypic plasticity by nucleotide substitutions initiates the process of genetic fixation of the novel trait. That is, while phenotypic plasticity plays a role as a buffer of evolution and contributes to environmental adaptation, as previously thought, our molecular data suggest that mutation on CpG site causing the loss of phenotypic plasticity, is the trigger for a generation of novel traits.

  23. メダカにおいてバソトシン系は配偶者防衛行動を制御する(Vasotocin system regulates mate-guarding behavior in medaka fish)

    Yokoi Saori, Okuyama Teruhiro, Ansai Satoshi, Kamei Yasuhiro, Taniguchi Yoshihito, Kinoshita Masato, Young Larry J., Takemori Nobuaki, Kubo Takeo, Naruse Kiyoshi, Takeuchi Hideaki

    比較生理生化学　33　(4)　149-149　2016/12

    Publisher: 日本比較生理生化学会

    ISSN： 0916-3786

    eISSN： 1881-9346

  24. Production of Knockout Mutants by CRISPR/Cas9 in the European Honeybee, Apis mellifera L. Peer-reviewed

    Hiroki Kohno, Shota Suenami, Hideaki Takeuchi, Tetsuhiko Sasaki, Takeo Kubo

    ZOOLOGICAL SCIENCE　33　(5)　505-512　2016/10

    DOI： 10.2108/zs160043 　

    ISSN： 0289-0003

  25. Ontogeny and Sexual Differences in Swimming Proximity to Conspecifics in Response to Visual Cues in Medaka Fish Peer-reviewed

    Yasuko Isoe, Yumi Konagaya, Saori Yokoi, Takeo Kubo, Hideaki Takeuchi

    ZOOLOGICAL SCIENCE　33　(3)　246-254　2016/06

    DOI： 10.2108/zs150213 　

    ISSN： 0289-0003

  26. Analysis of the Differentiation of Kenyon Cell Subtypes Using Three Mushroom Body-Preferential Genes during Metamorphosis in the Honeybee (Apis mellifera L.) Peer-reviewed

    Shota Suenami, Rajib Kumar Paul, Hideaki Takeuchi, Genta Okude, Tomoko Fujiyuki, Kenichi Shirai, Takeo Kubo

    PLOS ONE　11　(6)　e0157841　2016/06

    DOI： 10.1371/journal.pone.0157841 　

    ISSN： 1932-6203

  27. Mate-guarding behavior enhances male reproductive success via familiarization with mating partners in medaka fish. International-journal Peer-reviewed

    Saori Yokoi, Satoshi Ansai, Masato Kinoshita, Kiyoshi Naruse, Yasuhiro Kamei, Larry J Young, Teruhiro Okuyama, Hideaki Takeuchi

    Frontiers in zoology　13　21-21　2016

    DOI： 10.1186/s12983-016-0152-2 　

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    BACKGROUND: Male-male competition and female mating preference are major mechanisms of sexual selection, which influences individual fitness. How male-male competition affects female preference, however, remains poorly understood. Under laboratory conditions, medaka (Oryzias latipes) males compete to position themselves between a rival male and the female (mate-guarding) in triadic relationships (male, male, and female). In addition, females prefer to mate with visually familiar males. In the present study, to examine whether mate-guarding affects female preference via visual familiarization, we established a novel behavioral test to simultaneously quantify visual familiarization of focal males with females and mate-guarding against rival males. In addition, we investigated the effect of familiarization on male reproductive success in triadic relationships. RESULTS: Three fish (female, male, male) were placed separately in a transparent three-chamber tank, which allowed the male in the center (near male) to maintain closer proximity to the female than the other male (far male). Placement of the wild-type male in the center blocked visual familiarization of the far male by the female via mate-guarding. In contrast, placement of an arginine-vasotocin receptor mutant male, which exhibits mate-guarding deficits, in the center, allowing for maintaining close proximity to the female, did not block familiarization of the far male by the female. We also demonstrated that the reproductive success of males was significantly decreased by depriving females visual familiarization with the males. CONCLUSIONS: Our findings indicated that, at least in triadic relationships, dominance in mate-guarding, not simply close proximity, allows males to gain familiarity with the female over their rivals, which may enhance female preference for the dominant male. These findings focusing on the triadic relationships of medaka may contribute to our understanding of the adaptive significance of persistent mate-guarding, as well as female preference for familiar mates.

  28. [Exploring evolutionary roots of "social brain": Central role of terminal nerve GnRH3 neurons in social decision making]. Peer-reviewed

    Teruhiro Okuyama, Hideaki Takeuchi

    Seikagaku. The Journal of Japanese Biochemical Society　87　(5)　605-8　2015/10

    ISSN： 0037-1017

  29. Changes in the Gene Expression Profiles of the Hypopharyngeal Gland of Worker Honeybees in Association with Worker Behavior and Hormonal Factors Peer-reviewed

    Takayuki Ueno, Hideaki Takeuchi, Kiyoshi Kawasaki, Takeo Kubo

    PLOS ONE　10　(6)　e0130206　2015/06

    DOI： 10.1371/journal.pone.0130206 　

    ISSN： 1932-6203

  30. An Essential Role of the Arginine Vasotocin System in Mate-Guarding Behaviors in Triadic Relationships of Medaka Fish (Oryzias latipes) International-journal Peer-reviewed

    Saori Yokoi, Teruhiro Okuyama, Yasuhiro Kamei, Kiyoshi Naruse, Yoshihito Taniguchi, Satoshi Ansai, Masato Kinoshita, Larry J. Young, Nobuaki Takemori, Takeo Kubo, Hideaki Takeuchi

    PLOS GENETICS　11　(2)　e1005009-Takeuchi　2015/02

    DOI： 10.1371/journal.pgen.1005009 　

    ISSN： 1553-7404

  31. A neural mechanism underlying mating preferences for familiar individuals in medaka fish. International-journal Peer-reviewed

    Teruhiro Okuyama, Saori Yokoi, Hideki Abe, Yasuko Isoe, Yuji Suehiro, Haruka Imada, Minoru Tanaka, Takashi Kawasaki, Shunsuke Yuba, Yoshihito Taniguchi, Yasuhiro Kamei, Kataaki Okubo, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Yoshitaka Oka, Takeo Kubo, Hideaki Takeuchi

    Science (New York, N.Y.)　343　(6166)　91-4　2014/01/03

    Publisher: 6166

    DOI： 10.1126/science.1244724 　

    ISSN： 0036-8075

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    Social familiarity affects mating preference among various vertebrates. Here, we show that visual contact of a potential mating partner before mating (visual familiarization) enhances female preference for the familiarized male, but not for an unfamiliarized male, in medaka fish. Terminal-nerve gonadotropin-releasing hormone 3 (TN-GnRH3) neurons, an extrahypothalamic neuromodulatory system, function as a gate for activating mating preferences based on familiarity. Basal levels of TN-GnRH3 neuronal activity suppress female receptivity for any male (default mode). Visual familiarization facilitates TN-GnRH3 neuron activity (preference mode), which correlates with female preference for the familiarized male. GnRH3 peptides, which are synthesized specifically in TN-GnRH3 neurons, are required for the mode-switching via self-facilitation. Our study demonstrates the central neural mechanisms underlying the regulation of medaka female mating preference based on visual social familiarity.

  32. Genetic control of startle behavior in medaka fish. International-journal Peer-reviewed

    Satomi Tsuboko, Tetsuaki Kimura, Minori Shinya, Yuji Suehiro, Teruhiro Okuyama, Atsuko Shimada, Hiroyuki Takeda, Kiyoshi Naruse, Takeo Kubo, Hideaki Takeuchi

    PloS one　9　(11)　e112527　2014

    Publisher: 11

    DOI： 10.1371/journal.pone.0112527 　

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    Genetic polymorphisms are thought to generate intraspecific behavioral diversities, both within and among populations. The mechanisms underlying genetic control of behavioral properties, however, remain unclear in wild-type vertebrates, including humans. To explore this issue, we used diverse inbred strains of medaka fish (Oryzias latipes) established from the same and different local populations. Medaka exhibit a startle response to a visual stimulus (extinction of illumination) by rapidly bending their bodies (C-start) 20-ms after the stimulus presentation. We measured the rates of the response to repeated stimuli (1-s interval, 40 times) among four inbred strains, HNI-I, HNI-II, HO5, and Hd-rR-II1, and quantified two properties of the startle response: sensitivity (response rate to the first stimulus) and attenuation of the response probability with repeated stimulus presentation. Among the four strains, the greatest differences in these properties were detected between HNI-II and Hd-rR-II1. HNI-II exhibited high sensitivity (approximately 80%) and no attenuation, while Hd-rR-II1 exhibited low sensitivity (approximately 50%) and almost complete attenuation after only five stimulus presentations. Our findings suggested behavioral diversity of the startle response within a local population as well as among different populations. Linkage analysis with F2 progeny between HNI-II and Hd-rR-II1 detected quantitative trait loci (QTL) highly related to attenuation, but not to sensitivity, with a maximum logarithm of odds score of 11.82 on linkage group 16. The three genotypes (homozygous for HNI-II and Hd-rR-II1 alleles, and heterozygous) at the marker nearest the QTL correlated with attenuation. Our findings are the first to suggest that a single genomic region might be sufficient to generate individual differences in startle behavior between wild-type strains. Further identification of genetic polymorphisms that define the behavioral trait will contribute to our understanding of the neural mechanisms underlying behavioral diversity, allowing us to investigate the adaptive significance of intraspecific behavioral polymorphisms of the startle response.

  33. Correction: Novel Middle-Type Kenyon Cells in the Honeybee Brain Revealed by Area-Preferential Gene Expression Analysis. Peer-reviewed

    Kaneko K, Ikeda T, Nagai M, Hori S, Umatani C, Tadano H, Ugajin A, Nakaoka T, Paul RK, Fujiyuki T, Shirai K, Kunieda T, Takeuchi H, Kubo T

    PloS one　8　2013/11

    Publisher: 11

    DOI： 10.1371/annotation/1fa31a02-1b58-4361-98eb-5c213e5d5336 　

  34. Correction: A New Data-Mining Method to Search for Behavioral Properties That Induce Alignment and Their Involvement in Social Learning in Medaka Fish (Oryzias Latipes). Peer-reviewed

    Ochiai T, Suehiro Y, Nishinari K, Kubo T, Takeuchi H

    PloS one　8　2013/11

    Publisher: 11

    DOI： 10.1371/annotation/a895afc8-446c-4e3e-a71b-52f2baa9df10 　

  35. A New Data-Mining Method to Search for Behavioral Properties That Induce Alignment and Their Involvement in Social Learning in Medaka Fish (Oryzias Latipes) Peer-reviewed

    Takashi Ochiai, Yuji Suehiro, Katsuhiro Nishinari, Takeo Kubo, Hideaki Takeuchi

    PLOS ONE　8　(9)　e71685　2013/09

    DOI： 10.1371/journal.pone.0071685 　

    ISSN： 1932-6203

  36. Novel Middle-Type Kenyon Cells in the Honeybee Brain Revealed by Area-Preferential Gene Expression Analysis Peer-reviewed

    Kumi Kaneko, Tsubomi Ikeda, Mirai Nagai, Sayaka Hori, Chie Umatani, Hiroto Tadano, Atsushi Ugajin, Takayoshi Nakaoka, Rajib Kumar Paul, Tomoko Fujiyuki, Kenichi Shirai, Takekazu Kunieda, Hideaki Takeuchi, Takeo Kubo

    PLOS ONE　8　(8)　e71732　2013/08

    DOI： 10.1371/journal.pone.0071732 　

    ISSN： 1932-6203

  37. Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes. International-journal Peer-reviewed

    Teruhiro Okuyama, Yasuko Isoe, Masahito Hoki, Yuji Suehiro, Genki Yamagishi, Kiyoshi Naruse, Masato Kinoshita, Yasuhiro Kamei, Atushi Shimizu, Takeo Kubo, Hideaki Takeuchi

    PloS one　8　(6)　e66597　2013

    Publisher: 6

    DOI： 10.1371/journal.pone.0066597 　

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    BACKGROUND: Genetic mosaic techniques have been used to visualize and/or genetically modify a neuronal subpopulation within complex neural circuits in various animals. Neural populations available for mosaic analysis, however, are limited in the vertebrate brain. METHODOLOGY/PRINCIPAL FINDINGS: To establish methodology to genetically manipulate neural circuits in medaka, we first created two transgenic (Tg) medaka lines, Tg (HSP:Cre) and Tg (HuC:loxP-DsRed-loxP-GFP). We confirmed medaka HuC promoter-derived expression of the reporter gene in juvenile medaka whole brain, and in neuronal precursor cells in the adult brain. We then demonstrated that stochastic recombination can be induced by micro-injection of Cre mRNA into Tg (HuC:loxP-DsRed-loxP-GFP) embryos at the 1-cell stage, which allowed us to visualize some subpopulations of GFP-positive cells in compartmentalized regions of the telencephalon in the adult medaka brain. This finding suggested that the distribution of clonally-related cells derived from single or a few progenitor cells was restricted to a compartmentalized region. Heat treatment of Tg(HSP:Cre x HuC:loxP-DsRed-loxP-GFP) embryos (0-1 day post fertilization [dpf]) in a thermalcycler (39°C) led to Cre/loxP recombination in the whole brain. The recombination efficiency was notably low when using 2-3 dpf embyos compared with 0-1 dpf embryos, indicating the possibility of stage-dependent sensitivity of heat-inducible recombination. Finally, using an infrared laser-evoked gene operator (IR-LEGO) system, heat shock induced in a micro area in the developing brains led to visualization of clonally-related cells in both juvenile and adult medaka fish. CONCLUSIONS/SIGNIFICANCE: We established a noninvasive method to control Cre/loxP site-specific recombination in the developing nervous system in medaka fish. This method will broaden the neural population available for mosaic analyses and allow for lineage tracing of the vertebrate nervous system in both juvenile and adult stages.

  38. p53 Mutation suppresses adult neurogenesis in medaka fish (Oryzias latipes). International-journal Peer-reviewed

    Yasuko Isoe, Teruhiro Okuyama, Yoshihito Taniguchi, Takeo Kubo, Hideaki Takeuchi

    Biochemical and biophysical research communications　423　(4)　627-31　2012/07/13

    DOI： 10.1016/j.bbrc.2012.05.125 　

    ISSN： 0006-291X

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    Tumor suppressor p53 negatively regulates self-renewal of neural stem cells in the adult murine brain. Here, we report that the p53 null mutation in medaka fish (Oryzias latipes) suppressed neurogenesis in the telencephalon, independent of cell death. By using 5-bromo-29-deoxyuridine (BrdU) immunohistochemistry, we identified 18 proliferation zones in the brains of young medaka fish; in situ hybridization showed that p53 was expressed selectively in at least 12 proliferation zones. We also compared the number of BrdU-positive cells present in the whole telencephalon of wild-type (WT) and p53 mutant fish. Immediately after BrdU exposure, the number of BrdU-positive cells did not differ significantly between them. One week after BrdU-exposure, the BrdU-positive cells migrated from the proliferation zone, which was accompanied by an increased number in the WT brain. In contrast, no significant increase was observed in the p53 mutant brain. Terminal deoxynucleotidyl transferase (dUTP) nick end-labeling revealed that there was no significant difference in the number of apoptotic cells in the telencephalon of p53 mutant and WT medaka, suggesting that the decreased number of BrdU-positive cells in the mutant may be due to the suppression of proliferation rather than the enhancement of neural cell death. These results suggest that p53 positively regulates neurogenesis via cell proliferation.

  39. Neural mechanisms of female sexual preference in medaka Peer-reviewed

    Okuyama T, Yokoi S, Abe H, Taniguchi Y, Okubo K, Kamei Y, Shimada A, Naruse K, Takeda H, Oka Y, Kubo T, Takeuchi H

    Society for Neuroscience Abstract Viewer and Itinerary Planner　42　2012

  40. Ecdysteroid biosynthesis in workers of the European honeybee Apis mellifera L. Peer-reviewed

    Yurika Yamazaki, Makoto Kiuchi, Hideaki Takeuchi, Takeo Kubo

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY　41　(5)　283-293　2011/05

    DOI： 10.1016/j.ibmb.2011.01.005 　

    ISSN： 0965-1748

  41. Induction of c-fos transcription in the medaka brain (Oryzias latipes) in response to mating stimuli. International-journal Peer-reviewed

    Teruhiro Okuyama, Yuji Suehiro, Haruka Imada, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    Biochemical and biophysical research communications　404　(1)　453-7　2011/01/07

    Publisher: 1

    DOI： 10.1016/j.bbrc.2010.11.143 　

    ISSN： 0006-291X

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    Immediate-early genes (IEGs) are useful for mapping active brain regions in various vertebrates. Here we identified a c-fos homologue gene in medaka and demonstrated that the amounts of c-fos transcripts and proteins in the medaka brain increased in relation to an artificially evoked seizure, suggesting that the homologue gene has the characteristics of IEGs, which are used as markers of neural activity. Next, quantitative reverse-transcription-polymerase chain reaction revealed that female mating behaviors upregulated c-fos transcription in some brain regions including the telencephalon, optic tectum, and cerebellum. In addition, we performed in situ hybridization with a c-fos intron probe to detect the de novo synthesis of c-fos transcripts and confirmed induction of c-fos transcription in these brain regions after mating. This is the first report of IEG induction in response to mating stimuli in teleost fish. Our results indicated that c-fos expression was induced in response to behavioral stimuli in the medaka brain and that medaka c-fos could be a useful marker of neural activity.

  42. Neural mechanism for female mating preference of medaka mediated by visual information Peer-reviewed

    Teruhiro Okuyama, Yuji Suehiro, Haruka Imada, Hideki Abe, Atsuko Shimada, Minoru Tanaka, Kiyoshi Naruse, Hiroyuki Takeda, Yoshitaka Oka, Takeo Kubo, Hideaki Takeuchi

    NEUROSCIENCE RESEARCH　71　E267-E267　2011

    DOI： 10.1016/j.neures.2011.07.1166 　

    ISSN： 0168-0102

  43. Analysis of the molecular and neural mechanism of female mating acceptance depending on visual information in small fish medaka Peer-reviewed

    Okuyama T, Suehiro Y, Imada H, Shimada A, Abe H, Tanaka M, Naruse K, Takeda H, Oka Y, Kubo T, Takeuchi H

    Society for Neuroscience Abstract Viewer and Itinerary Planner　41　2011

  44. Expression of two microRNAs, ame-mir-276 and-1000, in the adult honeybee (Apis mellifera) brain Peer-reviewed

    Sayaka Hori, Kumi Kaneko, Takeshi H. Saito, Hideaki Takeuchi, Takeo Kubo

    APIDOLOGIE　42　(1)　89-102　2011/01

    DOI： 10.1051/apido/2010032 　

    ISSN： 0044-8435

    eISSN： 1297-9678

  45. Transient and permanent gene transfer into the brain of the teleost fish medaka (Oryzias latipes) using human adenovirus and the Cre-loxP system. International-journal Peer-reviewed

    Yuji Suehiro, Masato Kinoshita, Teruhiro Okuyama, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Mitsuhiro Hashimoto, Hideaki Takeuchi

    FEBS letters　584　(16)　3545-9　2010/08/20

    DOI： 10.1016/j.febslet.2010.06.047 　

    ISSN： 0014-5793

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    In this study, we demonstrated that human type-5 adenovirus infected the brain of the teleost fish, medaka (Oryzias latipes), in vivo. Injection of adenoviral vector into the mesencephalic ventricle of medaka larvae induced the expression of reporter genes in some parts of the telencephalon, the periventricular area of the mesencephalon and diencephalon, and the cerebellum. Additionally, the Cre-loxP system works in medaka brains using transgenic medaka carrying a vector containing DsRed2, flanked by loxP sites under control of the beta-actin promoter and downstream promoterless enhanced green fluorescent protein (EGFP). We demonstrated that the presence of green fluorescence depended on injection of adenoviral vector expressing the Cre gene and confirmed that EGFP mRNA was transcribed in the virus-injected larvae.

  46. Coordinated and cohesive movement of two small conspecific fish induced by eliciting a simultaneous optomotor response. International-journal Peer-reviewed

    Haruka Imada, Masahito Hoki, Yuji Suehiro, Teruhiro Okuyama, Daisuke Kurabayashi, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    PloS one　5　(6)　e11248　2010/06/22

    DOI： 10.1371/journal.pone.0011248 　

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    BACKGROUND: In animal groups such as herds, schools, and flocks, a certain distance is maintained between adjacent individuals, allowing them to move as a cohesive unit. Proximate causations of the cohesive and coordinated movement under dynamic conditions, however, have been poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: We established a novel and simple behavioral assay using pairs of small fish (medaka and dwarf pufferfish) by eliciting a simultaneous optomotor response (OMR). We demonstrated that two homospecific fish began to move cohesively and maintained a distance of 2 to 4 cm between them when an OMR was elicited simultaneously in the fish. The coordinated and cohesive movement was not exhibited under a static condition. During the cohesive movement, the relative position of the two fish was not stable. Furthermore, adult medaka exhibited the cohesive movement but larvae did not, despite the fact that an OMR could be elicited in larvae, indicating that this ability to coordinate movement develops during maturation. The cohesive movement was detected in homospecific pairs irrespective of body-color, sex, or albino mutation, but was not detected between heterospecific pairs, suggesting that coordinated movement is based on a conspecific interaction. CONCLUSIONS/SIGNIFICANCE: Our findings demonstrate that coordinated behavior between a pair of animals was elicited by a simultaneous OMR in two small fish. This is the first report to demonstrate induction of a schooling-like movement in a pair of fish by an OMR and to investigate the effect of age, sex, body color, and species on coordination between animals under a dynamic condition.

  47. Proliferation zones in adult medaka (Oryzias latipes) brain. International-journal Peer-reviewed

    Yoshiko Kuroyanagi, Teruhiro Okuyama, Yuji Suehiro, Haruka Imada, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    Brain research　1323　33-40　2010/04/06

    DOI： 10.1016/j.brainres.2010.01.045 　

    ISSN： 0006-8993

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    Cell proliferation in the adult mammalian brain is maintained at a low rate, but cell proliferation in the adult fish brain is prominent. To compare the distribution of proliferating cells among fish species, mutants, and under different growing environments, we mapped the zones of cell proliferation in the adult medaka (Oryzias latipes) brain and identified 17 proliferation zones in both male and female brains. These zones were distributed in the telencephalon (4 zones), preoptic area (2 zones), pineal body (1 zone), hypophysis (1 zone), habenular nucleus (1 zone), optic tectum (2 zones), third ventricular zone (1 zone), ventromedial nucleus (1 zone), hypothalamus (1 zone), and cerebellum (3 zones). Of the 17 zones, 16 corresponded to brain regions where cells proliferate in the zebrafish brain, suggesting that the persistence of the generation of new cells, at least in these zones, might be conserved among some fish species. We then compared the distribution of proliferation zones using two body-color mutant medaka, the T5 and Quintet, the latter of which is an albino mutant that completely lacks pigmentation. There was no apparent difference in the distribution pattern among these mutant strains. Finally, we compared these proliferation zones in the brains of isolated- and group-reared fish and detected no significant difference between the two groups. These findings demonstrate that there is persistent cell proliferation in at least these 16 zones of the adult medaka brain, irrespective of sex, body-color, and growth environment, suggesting that proliferation capacity in the 16 zones is maintained robustly in the adult medaka brain.

  48. Structural diversity and evolution of the N-terminal isoform-specific region of ecdysone receptor-A and-B1 isoforms in insects Peer-reviewed

    Takayuki Watanabe, Hideaki Takeuchi, Takeo Kubo

    BMC EVOLUTIONARY BIOLOGY　10　40　2010/02

    DOI： 10.1186/1471-2148-10-40 　

    ISSN： 1471-2148

  49. In Situ Hybridization Analysis of the Expression of Futsch, Tau, and MESK2 Homologues in the Brain of the European Honeybee (Apis mellifera L.) Peer-reviewed

    Kumi Kaneko, Sayaka Hori, Mai M. Morimoto, Takayoshi Nakaoka, Rajib Kumar Paul, Tomoko Fujiyuki, Kenichi Shirai, Akiko Wakamoto, Satomi Tsuboko, Hideaki Takeuchi, Takeo Kubo

    PLOS ONE　5　(2)　e9213　2010/02

    DOI： 10.1371/journal.pone.0009213 　

    ISSN： 1932-6203

  50. Age- and division-of-labour-dependent differential expression of a novel non-coding RNA, Nb-1, in the brain of worker honeybees, Apis mellifera L. Peer-reviewed

    H. Tadano, Y. Yamazaki, H. Takeuchi, T. Kubo

    INSECT MOLECULAR BIOLOGY　18　(6)　715-726　2009/12

    DOI： 10.1111/j.1365-2583.2009.00911.x 　

    ISSN： 0962-1075

  51. Distribution of Kakugo Virus and Its Effects on the Gene Expression Profile in the Brain of the Worker Honeybee Apis mellifera L. Peer-reviewed

    Tomoko Fujiyuki, Emiko Matsuzaka, Takayoshi Nakaoka, Hideaki Takeuchi, Akiko Wakamoto, Seii Ohka, Kazuhisa Sekimizu, Akio Nomoto, Takeo Kubo

    JOURNAL OF VIROLOGY　83　(22)　11560-11568　2009/11

    DOI： 10.1128/JVI.00519-09 　

    ISSN： 0022-538X

  52. Differential Gene Expression in the Hypopharyngeal Glands of Worker Honeybees (Apis mellifera L.) Associated with an Age-Dependent Role Change Peer-reviewed

    Takayuki Ueno, Takayoshi Nakaoka, Hideaki Takeuchi, Takeo Kubo

    ZOOLOGICAL SCIENCE　26　(8)　557-563　2009/08

    DOI： 10.2108/zsj.26.557 　

    ISSN： 0289-0003

  53. Mass spectrometric map of neuropeptide expression and analysis of the gamma-prepro-tachykinin gene expression in the medaka (Oryzias latipes) brain. International-journal Peer-reviewed

    Yuji Suehiro, Akikazu Yasuda, Teruhiro Okuyama, Haruka Imada, Yoshiko Kuroyanagi, Takeo Kubo, Hideaki Takeuchi

    General and comparative endocrinology　161　(1)　138-45　2009/03

    Publisher: 1

    DOI： 10.1016/j.ygcen.2008.12.001 　

    ISSN： 0016-6480

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    Neuropeptides have important roles in modulating behavioral patterns such as social interaction. With the aim to determine the presence of neuropeptides known to be involved in social interaction as well as novel peptides, we used MALDI-TOF/MS to analyze neuropeptide profiles in some medaka brain regions. In the telencephalon, hypothalamus, and pituitary gland, 3, 6, and 10 peaks, respectively, were identified as neuropeptides (Arg-vasotocin [AVT], growth hormone-releasing hormone [GHRH], neuropeptide FF, substance P [SP], somatostatin-1 and -2, melanin-concentrating hormone [MCH], MCH gene-related peptide [Mgrp], melanocyte-stimulating hormone [MSH], corticotropin-like intermediate lobe peptide [CLIP], and beta-endorphin). The neuropeptide profile of telencephalon similar to that of the hypothalamus, but completely different from that of pituitary gland. For the future genetic analysis, we identified cDNAs encoding precursor proteins for the identified peptides. We also detect its expression of gamma-prepro-tachykinin gene encoding a SP precursor protein in both the telencephalon and hypothalamus. Our results indicated that the medaka brain contains some neuropeptides (AVT, SP, and somatostatins) that may be involved in modulating medaka behaviors such as social interaction.

  54. [New approach toward understanding of neural basis of the honeybee "dance communication"]. Peer-reviewed

    Kiya T, Hori S, Takeuchi H, Kubo T

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　53　(11)　1368-1374　2008/09

    Publisher: 11

    ISSN： 0039-9450

  55. Gene expression profile in cerebrum in the filial imprinting of domestic chicks (Gallus gallus domesticus) Peer-reviewed

    Shinji Yamaguchi, Ikuko Fujii-Taira, Sachiko Katagiri, Ei-Ichi Izawa, Yasuyuki Fujimoto, Hideaki Takeuchi, Tatsuya Takano, Toshiya Matsushima, Koichi J. Homma

    BRAIN RESEARCH BULLETIN　76　(3)　275-281　2008/06

    DOI： 10.1016/j.brainresbull.2008.02.002 　

    ISSN： 0361-9230

  56. Laying workers in queenless honeybee (Apis mellifera L.) colonies have physiological states similar to that of nurse bees but opposite that of foragers Peer-reviewed

    Takayoshi Nakaoka, Hideaki Takeuchi, Takeo Kubo

    JOURNAL OF INSECT PHYSIOLOGY　54　(5)　806-812　2008/05

    DOI： 10.1016/j.jinsphys.2008.02.007 　

    ISSN： 0022-1910

  57. メダカの視運動反応の情報処理過程とその神経基盤の解析

    末廣勇司, 奥山輝大, 今田はるか, 成瀬清, 島田敦子, 武田洋幸, 久保健雄, 竹内秀明

    第18回 インテリジェントシステムシンポジウム講演論文集　501-504　2008

  58. Associative learning and discrimination of motion cues in the harnessed honeybee Apis mellifera L. Peer-reviewed

    Sayaka Hori, Hideaki Takeuchi, Takeo Kubo

    JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY　193　(8)　825-833　2007/08

    DOI： 10.1007/s00359-007-0234-x 　

    ISSN： 0340-7594

    eISSN： 1432-1351

  59. EcR-A expression in the brain and ovary of the honeybee (Apis mellifera L.) Peer-reviewed

    Hideaki Takeuchi, Rajib Kumar Paul, Emiko Matsuzaka, Takeo Kubo

    ZOOLOGICAL SCIENCE　24　(6)　596-603　2007/06

    DOI： 10.2108/zsj.24.596 　

    ISSN： 0289-0003

  60. Gene expression in the honeybee mushroom body and its gene orthologues

    Takeuchi, H.

    Evolution of Nervous Systems　1　2007

    DOI： 10.1016/B0-12-370878-8/00188-9 　

  61. Identification of proteins whose expression is up- or down-regulated in the mushroom bodies in the honeybee brain using proteomics Peer-reviewed

    Yuko Uno, Tomoko Fujiyuki, Mizue Morioka, Hideaki Takeuchi, Takeo Kubo

    FEBS LETTERS　581　(1)　97-101　2007/01

    DOI： 10.1016/j.febslet.2006.12.004 　

    ISSN： 0014-5793

  62. Expression of two ecdysteroid-regulated genes, Broad-Complex and E75, in the brain and ovary of the honeybee (Apis mellifera L.) Peer-reviewed

    Rajib Kumar Paul, Hideaki Takeuchi, Takeo Kubo

    ZOOLOGICAL SCIENCE　23　(12)　1085-1092　2006/12

    DOI： 10.2108/zsj.23.1085 　

    ISSN： 0289-0003

  63. Prevalence and phylogeny of Kakugo virus, a novel insect picorna-like virus that infects the honeybee (Apis mellifera L.), under various colony conditions Peer-reviewed

    Tornoko Fujiyuki, Seii Ohka, Hideaki Takeuchi, Masato Ono, Akio Nomoto, Takeo Kubo

    JOURNAL OF VIROLOGY　80　(23)　11528-11538　2006/12

    DOI： 10.1128/JVI.00754-06 　

    ISSN： 0022-538X

    eISSN： 1098-5514

  64. Carbohydrate metabolism genes and pathways in insects: insights from the honey bee genome Peer-reviewed

    T. Kunieda, T. Fujiyuki, R. Kucharski, S. Foret, S. A. Ament, A. L. Toth, K. Ohashi, H. Takeuchi, A. Kamikouchi, E. Kage, M. Morioka, M. Beye, T. Kubo, G. E. Robinson, R. Maleszka

    INSECT MOLECULAR BIOLOGY　15　(5)　563-576　2006/10

    DOI： 10.1111/j.1365-2583.2006.00677.x 　

    ISSN： 0962-1075

  65. Insights into social insects from the genome of the honeybee Apis mellifera Peer-reviewed

    George M. Weinstock, Gene E. Robinson, Richard A. Gibbs, Kim C. Worley, Jay D. Evans, Ryszard Maleszka, Hugh M. Robertson, Daniel B. Weaver, Martin Beye, Peer Bork, Christine G. Elsik, Klaus Hartfelder, Greg J. Hunt, Evgeny M. Zdobnov, Gro V. Amdam, Marcia M. G. Bitondi, Anita M. Collins, Alexandre S. Cristino, H. Michael G. Lattorff, Carlos H. Lobo, Robin F. A. Moritz, Francis M. F. Nunes, Robert E. Page, Zila L. P. Simoes, Diana Wheeler, Piero Carninci, Shiro Fukuda, Yoshihide Hayashizaki, Chikatoshi Kai, Jun Kawai, Naoko Sakazume, Daisuke Sasaki, Michihira Tagami, Stefan Albert, Geert Baggerman, Kyle T. Beggs, Guy Bloch, Giuseppe Cazzamali, Mira Cohen, Mark David Drapeau, Dorothea Eisenhardt, Christine Emore, Michael A. Ewing, Susan E. Fahrbach, Sylvain Foret, Cornelis J. P. Grimmelikhuijzen, Frank Hauser, Amanda B. Hummon, Jurgen Huybrechts, Andrew K. Jones, Tatsuhiko Kadowaki, Noam Kaplan, Robert Kucharski, Gerard Leboulle, Michal Linial, J. Troy Littleton, Alison R. Mercer, Timothy A. Richmond, Sandra L. Rodriguez-Zas, Elad B. Rubin, David B. Sattelle, David Schlipalius, Liliane Schoofs, Yair Shemesh, Jonathan V. Sweedler, Rodrigo Velarde, Peter Verleyen, Evy Vierstraete, Michael R. Williamson, Seth A. Ament, Susan J. Brown, Miguel Corona, Peter K. Dearden, W. Augustine Dunn, Michelle M. Elekonich, Tomoko Fujiyuki, Irene Gattermeier, Tanja Gempe, Martin Hasselmann, Tatsuhiko Kadowaki, Eriko Kage, Azusa Kamikouchi, Takeo Kubo, Robert Kucharski, Takekazu Kunieda, Marce D. Lorenzen, Natalia V. Milshina, Mizue Morioka, Kazuaki Ohashi, Ross Overbeek, Christian A. Ross, Morten Schioett, Teresa Shippy, Hideaki Takeuchi, Amy L. Toth, Judith H. Willis, Megan J. Wilson, Karl H. J. Gordon, Ivica Letunic, Kevin Hackett, Jane Peterson, Adam Felsenfeld, Mark Guyer, Michel Solignac, Richa Agarwala, Jean Marie Cornuet, Monique Monnerot, Florence Mougel, Justin T. Reese, Dominique Vautrin, Joseph J. Gillespie, Jamie J. Cannone, Robin R. Gutell, J. Spencer Johnston, Michael B. Eisen, Venky N. Iyer, Vivek Iyer, Peter Kosarev, Aaron J. Mackey, Victor Solovyev, Alexandre Souvorov, Katherine A. Aronstein, Katarina Bilikova, Yan Ping Chen, Andrew G. Clark, Laura I. Decanini, William M. Gelbart, Charles Hetru, Dan Hultmark, Jean-Luc Imler, Haobo Jiang, Michael Kanost, Kiyoshi Kimura, Brian P. Lazzaro, Dawn L. Lopez, Jozef Simuth, Graham J. Thompson, Zhen Zou, Pieter De Jong, Erica Sodergren, Miklos Csuroes, Aleksandar Milosavljevic, Kazutoyo Osoegawa, Stephen Richards, Chung-Li Shu, Laurent Duret, Eran Elhaik, Dan Graur, Juan M. Anzola, Kathryn S. Campbell, Kevin L. Childs, Derek Collinge, Madeline A. Crosby, C. Michael Dickens, L. Sian Grametes, Christina M. Grozinger, Peter L. Jones, Mireia Jorda, Xu Ling, Beverly B. Matthews, Jonathan Miller, Craig Mizzen, Miguel A. Peinado, Jeffrey G. Reid, Susan M. Russo, Andrew J. Schroeder, Susan E. St Pierre, Ying Wang, Pinglei Zhou, Huaiyang Jiang, Paul Kitts, Barbara Ruef, Anand Venkatraman, Lan Zhang, Gildardo Aquino-Perez, Charles W. Whitfield, Susanta K. Behura, Stewart H. Berlocher, Walter S. Sheppard, Deborah R. Smith, Andrew V. Suarez, Neil D. Tsutsui, Xuehong Wei, David Wheeler, Paul Havlak, Bingshan Li, Yue Liu, Erica Sodergren, Angela Jolivet, Sandra Lee, Lynne V. Nazareth, Ling-Ling Pu, Rachel Thorn, Viktor Stolc, Thomas Newman, Manoj Samanta, Waraporn A. Tongprasit, Charles Claudianos, May R. Berenbaum, Sunita Biswas, Dirk C. de Graaf, Rene Feyereisen, Reed M. Johnson, John G. Oakeshott, Hilary Ranson, Mary A. Schuler, Donna Muzny, Joseph Chacko, Clay Davis, Huyen Dinh, Rachel Gill, Judith Hernandez, Sandra Hines, Jennifer Hume, LaRonda Jackson, Christie Kovar, Lora Lewis, George Miner, Margaret Morgan, Ngoc Nguyen, Geoffrey Okwuonu, Heidi Paul, Jireh Santibanez, Glenford Savery, Amanda Svatek, Donna Villasana, Rita Wright

    NATURE　443　(7114)　931-949　2006/10

    DOI： 10.1038/nature05260 　

    ISSN： 0028-0836

    eISSN： 1476-4687

  66. Associative visual learning, color discrimination, and chromatic adaptation in the harnessed honeybee Apis mellifera L. Peer-reviewed

    Sayaka Hori, Hideaki Takeuchi, Kentaro Arikawa, Michiyo Kinoshita, Naoko Ichikawa, Masami Sasaki, Takeo Kubo

    JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY　192　(7)　691-700　2006/07

    DOI： 10.1007/s00359-005-0091-4 　

    ISSN： 0340-7594

    eISSN： 1432-1351

  67. Division of labor in the honey bee (Apis mellifera): the role of tyramine beta-hydroxylase Peer-reviewed

    Herman K. Lehman, David J. Schulz, Andrew B. Barron, Lydia Wraight, Chris Hardison, Sandra Whitney, Hideaki Takeuchi, Rajib K. Paul, Gene E. Robinson

    JOURNAL OF EXPERIMENTAL BIOLOGY　209　(14)　2774-2784　2006/07

    DOI： 10.1242/jeb.02296 　

    ISSN： 0022-0949

  68. Differential expression of HR38 in the mushroom bodies of the honeybee brain depends on the caste and division of labor Peer-reviewed

    Y Yamazaki, K Shirai, RK Paul, T Fujiyuki, A Wakamoto, H Takeuchi, T Kubo

    FEBS LETTERS　580　(11)　2667-2670　2006/05

    DOI： 10.1016/j.febslet.2006.04.016 　

    ISSN： 0014-5793

  69. Functional analysis of MbLK-1, a transcription factor preferentially expressed in the mushroom body of the honeybee brain Peer-reviewed

    Korefumi Nakamura, Takekazu Kunieda, Hideaki Takeuchi, Akiko Wakamoto, Takeo Kubo

    ZOOLOGICAL SCIENCE　22　(12)　1482-1482　2005/12

    ISSN： 0289-0003

  70. Cytochrome P450CYP307A1/spook: A regulator for ecdysone synthesis in insects Peer-reviewed

    T Namiki, R Niwa, T Sakudoh, K Shirai, H Takeuchi, H Kataoka

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　337　(1)　367-374　2005/11

    DOI： 10.1016/j.bbrc.2005.09.043 　

    ISSN： 0006-291X

  71. MBR-1, a novel helix-turn-helix transcription factor, is required for pruning excessive neurites in Caenorhabditis elegans Peer-reviewed

    E Kage, Y Hayashi, H Takeuchi, T Hirotsu, H Kunitomo, T Inoue, H Arai, Y Iino, T Kubo

    CURRENT BIOLOGY　15　(17)　1554-1559　2005/09

    DOI： 10.1016/j.cub.2005.07.057 　

    ISSN： 0960-9822

  72. Identification and characterization of P450 Cyp307a1 from the silkworm, Bombyx mori and the fruit fly, Drosophila melanogaster. Peer-reviewed

    T. Namiki, R. Niwa, K. Shirai, H. Takeuchi, H. Kataoka

    Biochem. Biophys. Res. Commun.　337, 367-374　2005

    DOI： 10.1016/j.bbrc.2005.09.043 　

  73. Gene expression of ecdysteroid-regulated gene E74 of the honeybee in ovary and brain Peer-reviewed

    RK Paul, H Takeuchi, Y Matsuo, T Kubo

    INSECT MOLECULAR BIOLOGY　14　(1)　9-15　2005/01

    DOI： 10.1111/j.1365-2583.2004.00524.x 　

    ISSN： 0962-1075

  74. [Molecular ethology using the honeybee as a model animal]. Peer-reviewed

    Takeuchi H, Kunieda T, Tokuhiro-Sawata M, Park JM, Fujiyuki T, Kubo T

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　49　(16)　2542-2548　2004/12

    Publisher: 16

    ISSN： 0039-9450

  75. Identification and analysis of the minimal promoter activity of a novel noncoding nuclear RNA gene, AncR-1, from the honeybee (Apis mellifera L.) Peer-reviewed

    M Sawata, H Takeuchi, T Kubo

    RNA-A PUBLICATION OF THE RNA SOCIETY　10　(7)　1047-1058　2004/07

    DOI： 10.1261/rna.5231504 　

    ISSN： 1355-8382

  76. Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera Peer-reviewed

    H Takeuchi, A Yasuda, Y Yasuda-Kamatani, M Sawata, Y Matsuo, A Kato, A Tsujimoto, T Nakajima, T Kubo

    CELL AND TISSUE RESEARCH　316　(2)　281-293　2004/05

    DOI： 10.1007/s00441-004-0865-y 　

    ISSN： 0302-766X

  77. Novel insect picorna-like virus identified in the brains of aggressive worker honeybees Peer-reviewed

    T Fujiyuki, H Takeuchi, M Ono, S Ohka, T Sasaki, A Nomoto, T Kubo

    JOURNAL OF VIROLOGY　78　(3)　1093-1100　2004/02

    DOI： 10.1128/JVI.78.3.1093-1100.2004 　

    ISSN： 0022-538X

  78. Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads Peer-reviewed

    H Takeuchi, A Yasuda, Y Yasuda-Kamatani, T Kubo, T Nakajima

    INSECT MOLECULAR BIOLOGY　12　(3)　291-298　2003/06

    DOI： 10.1046/j.1365-2583.2003.00414.x 　

    ISSN： 0962-1075

  79. Identification and characterization of Mlr1,2: two mouse homologues of Mblk-1, a transcription factor from the honeybee brain Peer-reviewed

    T Kunieda, JM Park, H Takeuchi, T Kubo

    FEBS LETTERS　535　(1-3)　61-65　2003/01

    DOI： 10.1016/S0014-5793(02)03858-9 　

    ISSN： 0014-5793

  80. Identification and punctate nuclear localization of a novel noncoding RNA, Ks-1, from the honeybee brain Peer-reviewed

    M Sawata, D Yoshino, H Takeuchi, A Kamikouchi, K Ohashi, T Kubo

    RNA-A PUBLICATION OF THE RNA SOCIETY　8　(6)　772-785　2002/06

    DOI： 10.1017/S1355838202028790 　

    ISSN： 1355-8382

  81. Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray Peer-reviewed

    H Takeuchi, T Fujiyuki, K Shirai, Y Matsuo, A Kamikouchi, Y Fujinawa, A Kato, A Tsujimoto, T Kubo

    FEBS LETTERS　513　(2-3)　230-234　2002/02

    DOI： 10.1016/S0014-5793(02)02319-0 　

    ISSN： 0014-5793

  82. DNA-binding properties of Mblk-1, a putative transcription factor from the honeybee Peer-reviewed

    JM Park, T Kunieda, H Takeuchi, T Kubo

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　291　(1)　23-28　2002/02

    DOI： 10.1006/bbrc.2002.6397 　

    ISSN： 0006-291X

  83. Identification of a novel gene, Mblk-1, that encodes a putative transcription factor expressed preferentially in the large-type Kenyon cells of the honeybee brain Peer-reviewed

    H Takeuchi, E Kage, M Sawata, A Kamikouchi, K Ohashi, M Ohara, T Fujiyuki, T Kunieda, K Sekimizu, S Natori, T Kubo

    INSECT MOLECULAR BIOLOGY　10　(5)　487-494　2001/10

    DOI： 10.1046/j.0962-1075.2001.00288.x 　

    ISSN： 0962-1075

  84. Concentrated expression of Ca2+/calmodulin-dependent protein kinase II and protein kinase C in the mushroom bodies of the brain of the honeybee Apis mellifera L Peer-reviewed

    A Kamikouchi, H Takeuchi, M Sawata, S Natori, T Kubo

    JOURNAL OF COMPARATIVE NEUROLOGY　417　(4)　501-510　2000/02

    DOI： 10.1002/(SICI)1096-9861(20000221)417:4<501::AID-CNE8>3.0.CO;2-4 　

    ISSN： 0021-9967

  85. Molecular biology concerning to the honeybee sociality: how can we approach the evolution of insect sociality?

    Kubo, T., Ohashi, K., Sawata, M., Kamikouchi, A., Takeuchi, H.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　45　(7)　2000

    ISSN： 0039-9450

  86. Soldier caste-specific gene expression in the mandibular glands of Hodotermopsis japonica (Isoptera : Termopsidae) Peer-reviewed

    T Miura, A Kamikouchi, M Sawata, H Takeuchi, S Natori, T Kubo, T Matsumoto

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　96　(24)　13874-13879　1999/11

    DOI： 10.1073/pnas.96.24.13874 　

    ISSN： 0027-8424

  87. Preferential expression of the gene for a putative inositol 1,4,5-trisphosphate receptor homologue in the mushroom bodies of the brain of the worker honeybee Apis mellifera L. Peer-reviewed

    A Kamikouchi, H Takeuchi, M Sawata, K Ohashi, S Natori, T Kubo

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　242　(1)　181-186　1998/01

    DOI： 10.1006/bbrc.1997.7870 　

    ISSN： 0006-291X

  88. Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II Peer-reviewed

    T Ito, QH Xu, H Takeuchi, T Kubo, S Natori

    FEBS LETTERS　385　(1-2)　21-24　1996/04

    DOI： 10.1016/0014-5793(96)00340-7 　

    ISSN： 0014-5793

  89. Molecular cloning of cDNA and analysis of expression of the gene for alpha-glucosidase from the hypopharyngeal gland of the honeybee Apis mellifera L Peer-reviewed

    K Ohashi, M Sawata, H Takeuchi, S Natori, T Kubo

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　221　(2)　380-385　1996/04

    DOI： 10.1006/bbrc.1996.0604 　

    ISSN： 0006-291X

  90. Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L) with age and/or role Peer-reviewed

    T Kubo, M Sasaki, J Nakamura, H Sasagawa, K Ohashi, H Takeuchi, S Natori

    JOURNAL OF BIOCHEMISTRY　119　(2)　291-295　1996/02

    ISSN： 0021-924X

Show all ︎Show first 5

Misc. 65

  1. 配偶者選択におけるオキシトシンの役割の性的二形性

    横井佐織, 成瀬清, 亀井保博, 安齋賢, 木下政人, 水戸麻理, 岩崎信太郎, 井ノ上俊太郎, 奥山輝大, 中川真一, YOUNG Larry J., 竹内秀明

    日本分子生物学会年会プログラム・要旨集(Web)　43rd　2020

  2. メダカの行動学 研究の最先端 Invited

    王 牧芸, 横井佐織, 奥山 輝大, 竹内 秀明

    生物の科学 遺伝　72　2018/11

  3. Neural mechanism underlying mate preference mediated by social memory

    奥山 輝大, 竹内 秀明

    生体の科学　67　(1)　71-75　2016/01

    Publisher: 金原一郎記念医学医療振興財団 ; 1949-

    DOI： 10.11477/mf.2425200403 　

    ISSN： 0370-9531

  4. Requirement of vasotocin for being a winner in love triangle relationships (two males and one female) of medaka fish Invited

    Saori Yokoi, Tatsuya Sakamoto, Hirotaka Sakamoto, Hideaki Takeuchi

    37　(6)　591-597　2015/12

    Publisher: 生物研究社

    ISSN： 0285-4376

  5. 「社会脳」の進化的起源の解明を目指して~社会的意思決定を担う終神経GnRH3ニューロン~

    奥山輝大, 竹内秀明

    生化学　87　(5)　605-608　2015/10/25

    DOI： 10.14952/SEIKAGAKU.2015.870605 　

    ISSN： 0037-1017

  6. 愛と分子(9)「異性の好み」を生み出す分子と神経 : 記憶・経験に基づく選択

    奥山 輝大, 竹内 秀明

    現代化学　(534)　28-31　2015/09

    Publisher: 東京化学同人

    ISSN： 0386-961X

  7. Exploring the neural geography of the social brain using medaka fish

    OKUYAMA TERUHIRO, TAKEUCHI HIDEAKI

    比較生理生化学　31　(3)　106-112　2014/09/30

    Publisher: 日本比較生理生化学会

    DOI： 10.3330/hikakuseiriseika.31.106 　

    ISSN： 0916-3786

    More details Close

    Within group-living animals, individuals appropriately tailor their attitudes and responses to other group members according to the social context and external environment. At the simplest level, the behavioral output can be described as approach and affiliation (positive response) versus agonistic behavior and avoidance (negative response). The neural substrate that works between sensory input and behavioral output, or the integrative circuits underlying decision-making processes, however, is vast and mysterious. To address this issue, we have focused on medaka fish, a model animal used mainly in the field of molecular genetics. Previously, we demonstrated that medaka females recognize familiar males following prior visual exposure, and social familiarity influences female mating receptivity. Medaka females exhibit a positive response (high receptivity) to familiar males, and a negative response (low receptivity) to unfamiliar males. Further, we demonstrated the essential role of a subpopulation of gonadotropin-releasing hormone-producing neurons (GnRH3 neurons) in switching from low to high female receptivity. Recently we established a new methodology for heat-inducible Cre/LoxP recombination in the medaka brain. Using the IR-LEGO system, heat shock induced in a very small area of the developing brains leads to spatially controlled recombination of progeny cells in adult medaka fish, which allows for genetic modulation and/or visualization of neuronal populations of interest. Using this system, medaka may serve as an ideal model animal to understand how the "social brain" works at molecular and neuronal levels.

  8. セイヨウミツバチApis mellifera L.脳でキノコ体に高度に選択的に発現する3遺伝子の同定

    末次翔太, 竹内秀明, 國枝武和, 久保健雄

    日本動物学会大会予稿集　84th　158　2013/08/12

  9. メダカの配偶者選択の分子神経基盤

    奥山輝大, 横井佐織, 阿部秀樹, 磯江泰子, 末廣勇司, 今田はるか, 島田敦子, 川崎隆史, 弓場俊輔, 谷口善仁, 亀井保博, 田中実, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明

    日本動物学会大会予稿集　84th　137　2013/08/12

  10. Analysis of neural mechanism for mate-guarding behavior, using small fish medaka

    Saori Yokoi, Teruhiro Okuyama, Hideaki Takeuchi, Takeo Kubo

    NEUROSCIENCE RESEARCH　71　E265-E265　2011

    DOI： 10.1016/j.neures.2011.07.1156 　

    ISSN： 0168-0102

  11. Neural basis underlying sexual selection in medaka fish

    Hideaki Takeuchi, Saori Yokoi, Yuji Suehiro, Haruka Imada, Minoru Tanaka, Takashi Kawasaki, Atsuko Shimada, Hideki Abe, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Teruhiro Okuyama

    NEUROSCIENCE RESEARCH　71　E24-E24　2011

    DOI： 10.1016/j.neures.2011.07.100 　

    ISSN： 0168-0102

  12. p53 mutation caused suppression of neurogenesis in the juvenile medaka fish brain

    Yasuko Isoe, Teruhiro Okuyama, Yoshihito Taniguchi, Takeo Kubo, Hideaki Takeuchi

    NEUROSCIENCE RESEARCH　71　E239-E240　2011

    DOI： 10.1016/j.neures.2011.07.1048 　

    ISSN： 0168-0102

  13. 視覚依存の配偶者選別において行動異常を示すメダカ変異体の同定

    奥山輝大, 末廣勇司, 今田はるか, 阿部秀樹, 島田敦子, 田中実, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明

    生化学　ROMBUNNO.1P-0845　2010

    ISSN： 0037-1017

  14. Analysis of the brain substructures using region-preferential genes of the honeybee

    2009/12

  15. Analysis of neural mechanism for the optomotor response of the medaka fish Peer-reviewed

    Yuji Suehiro, Teruhiro Okuyama, Haruka Imada, Kiyoshi Naruse, Daisuke Kurabayashi, Atsuko Shimada, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    Proceeding of the Third International Symposium on Mobiligence （査読有り国際紀要）　308-311　2009/11

  16. Analysis of Schooling-like Behavior of Medaka Fish and Identification of a Telencephalon-and-Hypothalamus-Preferential Gene in the Medaka Brain Peer-reviewed

    Haruka Imada, Yuji Suehiro, Teruhiro Okuyama, Daisuke Kurabayashi, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    Proceeding of the Third International Symposium on Mobiligence （査読有り国際紀要）　295-298　2009/11

  17. 2A1-E15 Distribution Shift and Task Allocation of Distributed Autonomous System by Double Repressing model

    HARUYAMA Hiroki, KURABAYASHI Daisuke, TAKEUCHI Hideaki

    2009　"2A1-E15(1)"-"2A1-E15(4)"　2009/05/25

    Publisher: The Japan Society of Mechanical Engineers

    More details Close

    Honeybee which is social insect changes the work with age in day and does division of labor in a colony. A feature of this division is one of the suitability of creatures, a model of controlling juvenile hormone of honeybee which increases with age in day was proposed as a conventional research. In this model, there are 2 repressing effects for juvenile hormone, one is generated in inside, the other is generated when contact with other honeybees. But contact is not defined because this model is not considered about spatial relation. So we expand the model to be considered about contact on a colony, and define 2 task modes with hormone content for express division of labor. Next, we derive local and whole rates of division of labor in a colony by analyzing equilibrium conditions of the hormone equations. And we verify that these rates have stability for disturbance.

  18. New approach toward understanding of neural basis of the honeybee "dance communication"

    Protein, nucleic acid and enzyme　53　(11)　1368-1374　2008/09

    Publisher: 共立出版

    ISSN： 0039-9450

  19. Differential expression of hormone receptor-like in 38 (HR38) in the honeybee brain depending on the caste differentiation and division of labor of the workers

    Y. Yamazaki, K. Shirai, R. K. Paul, T. Fujiyuki, A. Wakamoto, H. Takeuchi, T. Kubo

    FEBS JOURNAL　275　144-144　2008/06

    ISSN： 1742-464X

  20. Identification and analysis of genes that are expressed strongly in the optic lobes in the honeybee brain

    K. Kaneko, S. Hori, H. Takeuchi, R. K. Paul, T. Fujiyuki, K. Shirai, A. Wakamoto, T. Kubo

    FEBS JOURNAL　275　147-147　2008/06

    ISSN： 1742-464X

  21. G227 ミツバチ脳の視葉で主要に発現する2種類の遺伝子の解析

    金子九美, 堀沙耶香, 竹内秀明, PaulRajib Kumar, 藤幸知子, 白井健一, 若本明子, 久保健雄

    日本応用動物昆虫学会大会講演要旨　(52)　129-129　2008/03/12

    Publisher: 日本応用動物昆虫学会

  22. Analysis of Molecular/Neural Basis Underlying Information Processing Involved in Adaptive Behaviors (Optomotor Response and Schooling) of Small Fish

    TAKEUCHI Hideaki, SUEHIRO Yuji, IMADA Haruka, OKUYAMA Teruhiro, KUBO Takeo

    Journal of The Society of Instrument and Control Engineers　46　(12)　922-927　2008/01

    Publisher: The Society of Instrument and Control Engineers

    DOI： 10.11499/sicejl1962.46.922 　

    ISSN： 0453-4662

  23. 線虫C.elegansをモデルとした神経突起除去の分子機構の解析

    HAYASHI YU, HIROTSU TAKAAKI, NAKADAI ERIKO, TAKEUCHI HIDEAKI, KUNITOMO HIROFUMI, IINO YUICHI, KUBO TAKEO

    生化学　4P-0722　2007

    ISSN： 0037-1017

  24. Analysis of adaptive behaviors of small fishes by using combination of mathematical modeling and molecular biological approach. Peer-reviewed

    Suehiro Y, Imada H, Okuyama T, Kubo T, Takeuchi H†, †Corresponding

    Proceeding of the Second International Symposium on Mobiligence（査読有り国際紀要）.　219-222　2007

  25. Quantitative analysis of developmental processes of following behaviors in medaka using a newly established assay system

    Haruka Imada, Kenichi Shirai, Yuuji Suehiro, Kazuki Horikawa, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    ZOOLOGICAL SCIENCE　23　(12)　1187-1187　2006/12

    ISSN： 0289-0003

  26. Analysis of mathematical principle of information processing and the molecular/neural basis that underlie optomotor response of small fishes

    Yuuji Suehiro, Haruka Imada, Kazuki Horikawa, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    ZOOLOGICAL SCIENCE　23　(12)　1187-1187　2006/12

    ISSN： 0289-0003

  27. Physiological transition of honeybee workers induced by loss of the queen

    Takayoshi Nakaoka, Hideaki Takeuchi, Kenichi Shirai, Rajib Paul, Tomoko Fujiyuki, Akiko Wakamoto, Takeo Kubo

    ZOOLOGICAL SCIENCE　23　(12)　1225-1226　2006/12

    ISSN： 0289-0003

  28. Identification of candidate genes involved in social behavior in the honeybee brain. Using honeybee genome data base

    Hideaki Takeuchi

    ZOOLOGICAL SCIENCE　23　(12)　1129-1129　2006/12

    ISSN： 0289-0003

  29. Identification and analysis of genes that are expressed strongly in the optic lobes in the honeybee brain

    Kumi Kaneko, Sayaka Hori, Hideaki Takeuchi, Rajib Paul, Tomoko Fujiyuki, Kenichi Shirai, Akiko Wakamoto, Takeo Kubo

    ZOOLOGICAL SCIENCE　23　(12)　1191-1191　2006/12

    ISSN： 0289-0003

  30. Analysis of the significance and mechanism of neurite elimination using C-elegans as a model

    Yu Hayashi, Takaaki Hirotsu, Eriko Kage, Hideaki Takeuchi, Hirofumi Kunitomo, Yuichi Iino, Takeo Kubo

    NEUROSCIENCE RESEARCH　55　S185-S185　2006

    ISSN： 0168-0102

    eISSN： 1872-8111

  31. Prevalence of Kakugo virus in the honeybee Apis mellifera L. population analyzed by quantitative reverse transcription-PCR

    Tomoko Fujiyuki, Hideaki Takeuchi, Seii Ohka, Masato Ono, Akio Nomoto, Takeo Kubo

    ZOOLOGICAL SCIENCE　22　(12)　1482-1482　2005/12

    ISSN： 0289-0003

  32. Identification and analysis of genes whose expression change in the brains of the workers in queenless colonies

    Takayoshi Nakaoka, Hideaki Takeuchi, Takeo Kubo

    ZOOLOGICAL SCIENCE　22　(12)　1514-1514　2005/12

    ISSN： 0289-0003

  33. Identification of brain protein(s) expressed differentially in the worker honeybees in relation to their division of labors using proteome analysis

    Yuko Uno, Tomoko Fujiyuki, Mizue Morioka, Hideaki Takeuchi, Takeo Kuhn

    ZOOLOGICAL SCIENCE　22　(12)　1482-1482　2005/12

    ISSN： 0289-0003

  34. Acetylcholinesterase is queen-selectively transcribed in honeybee brain and ovary

    Yuichi Takeuchi, Hideaki Takeuchi, Maya Ohara, Kenta Asahina, Takeo Kubo

    ZOOLOGICAL SCIENCE　22　(12)　1510-1510　2005/12

    ISSN： 0289-0003

  35. 線虫 C.elegans をモデルとした神経突起除去の分子機構の解析

    HAYASHI YU, KAGE ERIKO, HIROTSU TAKAAKI, KUNITOMO HIROFUMI, TAKEUCHI HIDEAKI, IINO YUICHI, KUBO TAKEO

    日本分子生物学会年会講演要旨集　28th　294　2005/11/25

  36. 線虫において神経突起のせん断及びきゅう覚行動可塑性に関わる新規転写因子MBR‐1によって発現制御される遺伝子群の同定

    MITA YOSHIHISA, KAGE ERIKO, HAYASHI YU, KUNITOMO HIROFUMI, IINO YUICHI, KUBO TAKEO, TAKEUCHI HIDEAKI

    日本分子生物学会年会講演要旨集　28th　294　2005/11/25

  37. A318 光刺激-口吻伸展反射連合学習系を利用したセイヨウミツバチ(Apis mellifera L.)の波長識別能力と残像現象の解析(一般講演)

    堀沙耶香, 竹内秀明, 蟻川謙太郎, 木下充代, 佐々木正己, 市川直子, 久保健雄

    日本応用動物昆虫学会大会講演要旨　(49)　64-64　2005/03/01

    Publisher: 日本応用動物昆虫学会

  38. Kakugo virus from brains of aggressive worker honeybees

    T Fujiyuki, H Takeuchi, M Ono, S Ohka, T Sasaki, A Nomoto, T Kubo

    ADVANCES IN VIRUS RESEARCH, VOL 65　65　1-+　2005

    DOI： 10.1016/S0065-3527(05)65001-4 　

    ISSN： 0065-3527

  39. Analysis of molecular basis of associative visual learning in the honeybee Apis mellifera L.

    Sayaka Hori, Hideaki Takeuchi, Kentaro Arikawa, Michiyo Kinoshita, Masami Sasaki, Naoko Ichikawa, Takeo Kubo

    ZOOLOGICAL SCIENCE　21　(12)　1315-1316　2004/12

    ISSN： 0289-0003

  40. Identification and characterization of a novel gene, QM1, expressed preferentially in the brain of the queen honeybee (Apis mellifera L.)

    Kenichi Shirai, Maya Ohara, Hideaki Takeuchi, Takeo Kubo

    ZOOLOGICAL SCIENCE　21　(12)　1343-1343　2004/12

    ISSN： 0289-0003

  41. A novel C-elegans transcription factor MBR-1 is involved in olfactory plasticity and axon pruning

    Yu Hayashi, Eriko Kage, Takaaki Hirotsu, Hirofumi Kunitomo, Hideaki Takeuchi, Yuichi Iino, Takeo Kubo

    ZOOLOGICAL SCIENCE　21　(12)　1318-1318　2004/12

    ISSN： 0289-0003

  42. ミツバチをモデル生物とした分子行動生物学

    竹内秀明, 國枝武和, 徳廣, 澤, 美由紀

    蛋白質核酸酵素　49　(16)　2542-2548　2004/12

    Publisher: 共立出版

    ISSN： 0039-9450

  43. 線虫C.elegansの嗅覚可塑性に関わる転写因子MBR-1の軸索除去における機能解析(Analysis of the function of MBR-1,a C.elegans transcription factor involved in olfactory plasticity,in axon pruning)

    林 悠, 鹿毛 枝里子, 広津 崇亮, 國友 博文, 竹内 秀明, 飯野 雄一, 久保 健雄

    神経化学　43　(2-3)　526-526　2004/08

    Publisher: 日本神経化学会

    ISSN： 0037-3796

  44. セイヨウミツバチにおける光刺激-口吻伸展反射連合学習．

    堀沙耶香, 竹内秀明, 市川直子, 佐々木正己, 久保健雄

    日本応用動物昆虫学会大会講演要旨．　114　2004

  45. セイヨウミツバチの攻撃的な働き蜂の脳から同定されたKakugoウイルス

    藤幸知子, 竹内秀明, 久保健雄

    ミツバチ科学　25　(4)　145-151　2004

    Publisher: 玉川大学ミツバチ科学研究施設

    ISSN： 0388-2217

  46. 線虫 C.elegans のきゅう覚可塑性に関わる転写因子MBR‐1の軸索形成における機能解析

    HAYASHI YU, KAGE ERIKO, HIROTSU TAKAAKI, KUNITOMO HIROFUMI, TAKEUCHI HIDEAKI, IINO YUICHI, KUBO TAKEO

    日本分子生物学会年会プログラム・講演要旨集　26th　898　2003/11/25

  47. IDENTIFICATION OF HONEYBEE BRAIN REGION-SELECTIVE GENES BY A COMBINATION OF DIFFERENTIAL DISPLAY METHOD AND cDNA MICROARRAY(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Kumar Paul Rajib, Takeuchi Hideaki, Fujiyuki Tomoko, Matsuo Yuko, Kato Azusa, Tsujimoto Atsumi, Kubo Takeo

    Zoological science　20　(12)　1581-1581　2003

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  48. PREPRO-TACHYKININ GENE EXPRESSION IN THE HONEYBEE APIS MELLIFERA L. BRAIN(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Takeuchi Hideaki, Yasuda Akikazu, Yasuda Yoshimi, Matsuo Yuko, Kato Azusa, Tsujimoto Atsumi, Nakajima Terumi, Kubo Takeo

    Zoological science　20　(12)　1588-1588　2003

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  49. ASSOCIATIVE VISUAL LEARNING IN HONEYBEES APIS MELLIFERA L.(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Hori Sayaka, Takeuchi Hideaki, Sasaki Masami, Ichikawa Naoko, Kubo Takeo

    Zoological science　20　(12)　1587-1587　2003

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  50. IDENTIFICATION AND CHARACTERIZATION OF A NOVEL GENE, TERMED QM1, EXPRESSED PREFERENTIALLY IN THE BRAIN OF THE QUEEN HONEYBEE (APIS MELLIFERA L.)(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Shirai Kenichi, Ohara Maya, Takeuchi Hideaki, Kubo Takeo

    Zoological science　20　(12)　1587-1587　2003

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  51. セイヨウミツバチにおける光刺激-PER連合学習

    堀沙耶香, 竹内秀明, 佐々木正己, 市川直子, 久保健雄

    日本応用動物昆虫学会大会講演要旨．　93　2003

  52. A HONEYBEE (APIS MELLIFERA L.) BRAIN MAP DISSECTED AT THE MOLECULAR LEVEL USING DIFFERENTIAL DISPLAY AND DNA MICROARRAY METHOD(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Takeuchi Hideaki, Rajib Kumar Paul, Fujiyuki Tomoko, Matsuo Yuko, Shirai Kennichi, Kato Azusa, Tsujimoto Atsumi, Kubo Takeo

    Zoological science　19　(12)　1472-1472　2002

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  53. IDENTIFICATION AND CHARACTERIZATION OF A NOVEL NUCLEAR RNA FACTOR, KS-1, EXPRESSED IN THE HONEYBEE BRAIN(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Sawata Miyuki, Yoshino Daisuke, Takeuchi Hideaki, Kamikouchi Azusa, Ohashi Kazuaki, Kubo Takeo

    Zoological science　19　(12)　1472-1472　2002

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  54. IDENTIFICATION OF A NOVEL PICORNA-LIKE VIRUS GENOME RNA IN THE BRAIN OF THE AGGRESSIVE HONEYBEES(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Fujiyuki Tomoko, Takeuchi Hideaki, Ono Masato, Ohka Seii, Nomoto Akio, Sasaki Tetsuhiko, Kubo Takeo

    Zoological science　19　(12)　1472-1472　2002

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  55. Identification of two mouse homologs of Mblk-1, that encodes a mushroom body-specific transcription factor of the honeybee.(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Kunieda T., Takeuchi H., Kage E., Park J., Kubo T.

    Zoological science　18　106-106　2001

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  56. Characterization of the C. elegans homolog of Mblk-1 encoding a putative transcription factor that is expressed in the honey bee brain(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Kage Eriko, Takeuchi Hideaki, Inoue Takao, Suzuki Yuka, Sugimoto Asako, Yamamoto Masayuki, Kubo Takeo

    Zoological science　18　106-106　2001

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  57. Identification and characterization of genes induced in brains of the honeybees that have attacked the hornets.(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Fujiyuki T., Ono M., Takeuchi H., Kubo T.

    Zoological science　18　106-106　2001

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  58. Identification of a novel gene, termed QM1, expressed preferentially in the brain of the queen honeybee(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Shirai K., Ohara M., Takeuchi H., Kubo T.

    Zoological science　18　106-106　2001

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  59. Identification of the gene for a putative transcription factor (Mblk-1) expressed preferentially in the large-type Kenyon cells of the honey bee brain(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Takeuchi H., Kage E., Kamikouchi A., Kubo T.

    Zoological science　18　91-91　2001

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  60. ミツバチの高次行動を支える脳の分子機構の解析

    竹内秀明, 上川内あづさ, 鹿毛枝理子, 久保健雄

    ミツバチ科学　22　(3)　113-120　2000/12

    Publisher: 玉川大学

    ISSN： 0388-2217

  61. ミツバチの社会性をめぐる分子生物学——昆虫の社会性の進化にどのようにアプローチするか

    久保健雄, 大橋一晶, 澤田美由紀, 上川内あづさ, 竹内秀明

    蛋白質核酸酵素　45　(7)　1229-1236　2000/05

    Publisher: 7

    ISSN： 0039-9450

  62. Identification and analysis of the gene which selectively appears to the mushroom body of the honeybee.

    TAKEUCHI Hideaki, KAMIKOUCHI Azusa, OHARA Maya, SAWATA Miyuki, YOSHINO Daisuke, KUBO Takeo

    Dobutsu seiri　16　(4)　266-277　1999/12

    Publisher: THE JAPANESE SOCIETY FOR COMPARATIVE PHYSIOLOGY AND BIOCHEMISTRY

    DOI： 10.3330/hikakuseiriseika.16.266 　

    ISSN： 0916-3786 1881-9346

  63. Molecular Sociobiology of the Honeybee

    KUBO Takeo, OHASHI Kazuaki, TAKEUCHI Hideaki, SAWATA Miyuki, KAMIKOUCHI Azusa, NATORI Shunji

    KAGAKU TO SEIBUTSU　34　(12)　793-798　1997/01/25

    Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry

    DOI： 10.1271/kagakutoseibutsu1962.34.793 　

    ISSN： 0453-073X

  64. セイヨウミツバチ(Apis mellifera)脳のキノコ体のLarge type Kenyon細胞に特異的に発現する遺伝子の同定と解析

    竹内 秀明, 大橋 一晶, 澤田 美由紀, 名取 俊二, 久保 健雄

    日本分子生物学会年会プログラム・講演要旨集　19　528-528　1996/08/01

  65. ミツバチ働き蜂の行動変化に伴う下咽頭腺由来ロイヤルゼリー蛋白およびα-グルコシダーゼの遺伝子発現解析

    大橋 一晶, 竹内 秀明, 澤田 美由紀, 名取 俊二, 久保 健雄

    日本分子生物学会年会プログラム・講演要旨集　19　827-827　1996/08/01

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Books and Other Publications 7

  1. Perspectives on the Function of Oxytocin in Fish and Mammals (Chapter7)

    Yokoi S, Young LJ, Takeuchi H

    "Spectrum of Sex - The Molecular Bases that Induce Various Sexual Phenotypes" edited by M Tanaka and M Tachibana, Springer Nature　2022

  2. 動物行動の分子生物学 (新・生命科学シリーズ)

    久保 健雄, 上川内 あづさ, 竹内 秀明, 奥山 輝大, 太田 次郎, 浅島 誠, 長田 敏行, 赤坂 甲治

    裳華房　2014/07/30

    ISBN: 4785358580

  3. 身近な動物を使った実験〈4〉ミツバチ・コオロギ・スズメガ

    三共出版　2009/10

    ISBN: 4782705832

  4. 分子昆虫学 ―ポストゲノムの昆虫研究―

    共立出版　2009/08/25

    ISBN: 4320056957

  5. 昆虫ミメティックス―昆虫の設計に学ぶ (Advanced Biomimetics Series)

    下澤 楯夫, 針山 孝彦

    エヌティーエス　2008/10/10

    ISBN: 4860431979

  6. 生物薬科学実験講座「タンパク質」4.cDNAクローニング」

    廣川書店　2008/04

    ISBN: 9784567170109

  7. “Gene expression in the Honeybee Brain Mushroom Body and its Gene Orthologues” Evolution of Nervous Systems

    Hideaki Takeuchi, Edited by Kaas H

    Elsevier　2006/12

    ISBN: 9780123925602

    More details Close

    http://japan.elsevier.com/products/books/ENS.pdf

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Presentations 61

  1. My Research Strategy for Medaka fish: A Lesson from Larry Invited

    Hideaki Takeuchi

    International Symposium on the Social Brain Tribute to Dr. Larry J. Young　2025/03/26

  2. The role of oxytocin in social preference of medaka fish Invited

    Hideaki Takeuchi

    Molecular Neurobiology of Social Behavior: from animal model to human life　2024/11/27

  3. Elucidating the behavioral choice and molecular neural basis through visually-mediated social cognition in medaka fish. Invited

    Hideaki Takeuchi

    Social Memory : Neural Basis of Communication　2024/09/05

  4. Exploring Medaka Visual Mate Preference and its Diversity From Molecular Mechanisms to Evolutionary Neuroscience Invited

    Hideaki Takeuchi

    The 3rd Taiwan Society for Neuroscience Meeting　2024/08/25

  5. Clonal unit lineages construct the anatomical compartments of the medaka telencephalon Invited

    Yasuko Isoe, Hideaki Takeuchi

    2024/06/19

  6. 魚類の配偶者選択機構 Invited

    竹内秀明

    ジェンダード・イノベーション入門/お茶の水女子大学・東京大学・東北大学連携　2024/02/13

  7. Elucidating the behavioral choice and molecular neural basis through visually-mediated social cognition in medaka fish. Invited

    2023/10/09

  8. 研究ツールとしてのメダカ Invited

    東北大学Research Showcase vol.1 研究ツールとしての生物モデル ～ "これまで"の活用例と"これから"の可能性 ～　2023/07/04

  9. メダカのオスはどのようにメスの気を引くのか？ Invited

    竹内秀明

    世界脳週間イベント2022 主催：東北大学脳科学センター　2023/03/21

  10. Exploring the neural geography of the fish social brain Invited

    Hideaki Takeuchi

  11. Exploring the neural geography of the fish social brain Invited

    Commemorative Symposium for the 38th International Prize for Biology "Biology of Fishes: Ecology, Evolution and Development"

  12. メダカの視覚的な社会認知を介した行動選択とその分子神経基盤の解明 Invited

    竹内秀明

    第48回日本神経内分泌学会学術集会　2022/10/29

  13. Exploring the neural geography of the social brain using medaka fish Invited

    Hideaki Takeuchi

    2022 The International Congress Neuroethology (Lisbon)

  14. メダカの三角関係を生み出すホルモンと脳の仕組み Invited

    竹内秀明

    世界脳週間オンラインサイエンスカフェ　2022/03/13

  15. Future Aspects of Social Neuroscience Using Medaka Fish

    Hideaki Takeuchi

    社会脳シンポジウム：ポストコロナ時代の社会神経科学　2021/09/30

  16. メダカの個体認知を介した配偶者選択に関わる脳の分子神経基盤 Invited

    竹内秀明

    日本化学会　2021/03/19

  17. 性的パートナー選択にバイアスを与える脳の内部状態の神経基盤 Invited

    竹内秀明

    日本認知科学会第36回大会　2019/09/05

  18. メダカの恋の分子神経基盤 －恋ごころの進化的ルーツを探る－ Invited

    Hideaki Takeuchi

    日本科学者会議岡山支部例会よもやま話の会　2016/02/29

  19. Exploring the neural geography of the social brain using medaka fish International-presentation Invited

    Hideaki Takeuchi, Keynote Lecture

    17th Australia & New Zealand Zebrafish Meeting　2016/02/05

  20. Exploring the neural geography of the social brain using medaka fish International-presentation Invited

    Hideaki Takeuchi, Keynote Lecture

    3rd Medaka Strategic Meeting, Flinders, Australia　2016/02/01

  21. Neural mechanism of female preference mediated by social memory International-presentation Invited

    Hideaki Takeuchi

    Symposium on Memory and Mind, Tohoku Forum for Creativity　2015/09/29

  22. Molecular basis underlying Love triangle relationship Invited

    Hideaki Takeuchi

    Takeuchi　2015/09/19

  23. Molecular basis underlying Love triangle relationship Invited

    Hideaki Takeuchi

    Takeuchi　2015/08/01

  24. Proximate and ultimate causes of socially-regulated mating preference in medaka fish International-presentation Invited

    Hideaki Takeuchi

    2nd Strategic Meeting for Medaka Research　2014/04/10

  25. Socially-regulated mating preference in medaka fish and its neural mechanism International-presentation Invited

    Hideaki Takeuchi

    The 35th Annual Meeting of The Japanese Society for Comparative Physiology and Biochemistry 2013　2013/07/13

  26. Integrative analysis of sexual behaviors in medaka fish: From neurons to behavioral evolution Invited

    Hideaki Takeuchi

    "Evolution of the Brain” supported by CPIS, SOKENDAI　2012/11/13

  27. メダカから探る恋心の神経機構 Invited

    竹内秀明

    第22回東京大学理学部公開講演会 「三者三様 理学が挑む」　2012/11/04

  28. Neural Basis Underlying Sexual Selection in Medaka Fish International-presentation Invited

    Hideaki Takeuchi

    2012/01

  29. Neural Basis Underlying Sexual Selection in Medaka Fish International-presentation Invited

    Hideaki Takeuchi

    The 1st Strategic Meeting for Medaka Research　2011/11/23

  30. Neural basis underlying sexual selection in medaka fish International-presentation Invited

    Hideaki Takeuchi

    The 34th Annual Meeting of the Japan Neuroscience Society "Vertebrate brain functions elucidated in zebrafish and medaka"　2011/09/15

  31. Neural basis underlying female mating preference in medaka fish International-presentation Invited

    Hideaki Takeuchi

    the 8th International Congress on Comparative Physiology and Biochemistry　2011/06/02

  32. 配偶行動の分子神経基盤：実験動物の研究からヒトの 恋愛感情はどこまで理解できるのか？

    竹内秀明

    基礎生物学研究所重点共同利用シンポジウム　2011/03

  33. 「サービス・イノベーションへの期待」 International-presentation

    竹内秀明, パネリスト

    「理学は実業の問題を解決できるか」　2011/02

  34. Analysis of Social behaviors in Adult Medaka Fish International-presentation Invited

    竹内秀明

    The BRH Seminar "Evolution & Diversity of Fishes"　2010/06

  35. メダカの社会性行動を生み出す脳機能の解析 Invited

    竹内秀明

    日本分子生物学会春季シンポジウム「身体・脳システムの心理表出行動と分子動態基盤」　2010/06

  36. Molecular/ neural basis involved in social interaction among medaka fish

    竹内秀明

    第87回日本生理学会大会「比較的単純なモデル動物を用いた運動・行動研究」　2010/05

  37. Analysis of Social Interactions Using Adult Medaka Fish Invited

    Hideaki Takeuchi

    Common Themes and New Concepts in Sensory Formation, The 18th CDB Meeting.　2009/04

  38. メダカの個体間相互作用を定量する行動実験系の確立

    竹内秀明

    北大行動知能講演会　2009/03

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    メダカは日本国内で江戸時代からペットとして飼育され、「メダカの学校」と呼ばれる群れ行動や攻撃行動、また雄の求愛円舞（配偶行動）など多様な個体間相互作用の様式が観察されている。一方でメダカは発生や生殖生物学のモデル生物として遺伝子導入法やノックアウトの作出など洗練された分子遺伝学的手法が確立している。私達は将来的にメダカの行動様式に関わる脳機能を分子遺伝学的手法で解析する目的で、メダカの個体間相互作用を定量化する行動実験系の確立を行っている。これまでにメダカの配偶行動における雄と雌の性的動機を定量化する行動アッセイ系や二匹のメダカの同調的行動を定量化する行動アッセイ系を確立した。本講演ではこれらの個体間相互作用に影響を与える要因やその生物学的意義について議論していきたいと考えている。

  39. セイヨウミツバチ脳におけるエクダイソンに関わる遺伝子群の発現様式 Invited

    竹内秀明

    第53回日本応用動物昆虫学会大会　2009/03

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    第１回昆虫ホルモン談話会~幼若ホルモンとエクジソンの生理機能研究の新展開~

  40. Analysis of information processing and neural/ molecular basis underlying adaptive behaviors (optomotor response and coordinated behavior) of small fish

    Hideaki Takeuchi

    第18回インテリジェント・システム・シンポジウム　2008/10

  41. Analysis of Social Interactions Using Adult Medaka Fish International-presentation Invited

    Hideaki Takeuchi

    The 54th NIBB Conference, New Frontiers for the Medaka Model, Genome, Bioresources and Biology　2008

  42. ミツバチとショウジョウバエの比較分子神経生物学

    竹内秀明

    生研センターシンポジウム　2007/07

  43. ミツバチの社会性行動とゲノムデーターベース

    Hideaki Takeuchi

    ニューロエソロジー談話会　2006/09

  44. Molecular basis underlyning the mushroom body function of the honeybee brain Invited

    Hideaki Takeuchi

    Bee Vision and Learning, SECOND EUROPEAN CONFERENCE OF APIDOLOGY , Praha, Czech Republic　2006/09

  45. Candidate genes involved in honeybee social behaviors Invited

    Hideaki Takeuchi

    Special Talk at Centre de Recherches sur la Cognition Animale CNRS - Université Paul Sabatier - Toulouse III, France　2006/09

  46. Candidate genes involved in honeybee social behaviors Invited

    Hideaki Takeuchi

    Special Talk at Lehrstuhl für Neurobiologie Universität Konstanz, Germany　2006/03

  47. Gene expression in honeybee mushroom bodies and its gene orthologues in non-social insects/invertebrates International-presentation Invited

    Hideaki Takeuchi

    3rd European Congress on Social Insects, Meeting of European Sections of the IUSSI, St. Petersburg, Russia　2005/08

  48. Honeybee as a new model system for molecular neurobiology International-presentation Invited

    Hideaki Takeuchi

    第77回日本遺伝学会 公開講演会：神経遺伝学の現状と展望　2005

  49. Future aspect of molecular neurobiology using a honeybee as a model system International-presentation Invited

    Hideaki Takeuchi

    The Gordon Research Conference, Neuroethology: Behavior, Evolution & Neurobiology (Oxford, United Kingdom)　2005

  50. Honeybee as a new model system for molecular neurobiology International-presentation

    Hideaki Takeuchi

    Special Talk at Institute of Zoology, Department of Animal Physiology, University of Cologne, Germany　2005

  51. Future aspect of molecular neurobiology using a honeybee mushroom body as a model system

    Hideaki Takeuchi

    Neuro 2004　2004

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    日本神経化学会大会、日本神経科学大会、合同大会

  52. Honeybee as a new model system for molecular neurobiology Invited

    Hideaki Takeuchi

    International Symposium on Chemical Senses and Insect Behavior　2004

  53. Honeybee as a new model system for molecular neurobiology Invited

    Hideaki Takeuchi

    Special Talk at Arizona Research Laboratories Division of Neurobiology, Arizona University　2004

  54. Honeybee as a new model system for molecular neurobiology

    Hideaki Takeuchi

    Special Talk at San Diego University of California　2004

  55. ミツバチが拓く脳分子生物学の未来

    竹内秀明

    基生研研究会「新しいモデル生物が拓く生物科学フロンティア」　2004

  56. Molecular basis underlying honeybee social behavior.

    Hideaki Takeuchi

    International Union of Biological Sciences - Towards An Integrative Biology Symposium on "Biology of Learning"　2003

  57. Molecular dissection of the mushroom body in the honeybee brain

    Hideaki Takeuchi

    Special Talk at Department of Entomology, University of Illinois　2003

  58. ミツバチ脳において高次中枢（キノコ体）で機能する遺伝子群の同定

    竹内秀明

    Zoological Conference, 東京大学大学院理学系講義　2003

  59. オスとメスの「恋ごころ」の違いを動物の脳の仕組みから考える Invited

    竹内秀明

    世界脳週間イベント2023 主催：東北大学脳科学センター、共催：東北大学大学院生命科学研究科　2024/03/20

  60. 最先端技術で迫る脳の働きと多様性 Invited

    竹内秀明

    適応回路センサスに基づく 適応機能の構築と遷移バイオメカニズム 令和5年度 公開シンポジウム　2024/03/10

  61. Study on the molecular/ neural mechanism of how the brain and behavioral state influences social-decision making using medaka fish as an animal model. Invited

    2020/08/22

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Research Projects 29

  1. 脳バリアシステムを介した行動変容機構の解明

    竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 学術変革領域研究(A)

    Institution: 東北大学

    2025/04/01 - 2027/03/31

  2. 視覚シグナルを介した種内コミュニケーションの脳の分子神経基盤の解明

    竹内 秀明, 安齋 賢

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2024/06 - 2026/03

  3. 視覚を介した配偶者選択における脳の性差と進化の分子基盤の解明

    竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 学術変革領域研究(A)

    Institution: 東北大学

    2024/04 - 2026/03

  4. A neural mechanism underlying female mate preference using a ornamental signal

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    2023/04 - 2026/03

  5. Molecular/genetic basis underlying the fish social vision

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2021/04 - 2025/03

  6. 3次元モダリティー解析法による配偶戦略の性差を生み出す分子神経基盤の解明

    竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 学術変革領域研究(A)

    Institution: 東北大学

    2022/06/16 - 2024/03/31

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    2022年度はメダカの終脳と視蓋のオスとメスの両方のサンプルを用いて実験を行い、細胞分離過程のストレスで最初期遺伝子の発現が誘導されない条件で、メダカ脳のscRNA-seqの実施に成功した。方法は摘出したメダカ終脳を低温条件下のプロテアーゼ（真菌由来のプロテアーゼ）処理後、物理的な攪拌（先の開いたチップによるピペッテング）により１細胞レベルに分離した（細胞生存率80%以上）。分離した細胞を10X Genomics Chromiumにより、分取、バーコーディング、ライブラリー作製を行った。そして次世代シークエンサーを用いて3’部分配列のシーケンス（バーコード26塩基+125塩基）を行った。結果はメス・終脳（7,383細胞）、メス・視蓋（5,346細胞）、オス・終脳（7,078細胞）、オス・視蓋（8,597細胞）を回収し、そのトランスクリプトームデータを得ることに成功した。また1細胞あたりのリード数は41,694リード/cell（メス・終脳）、57,513リード/cell（メス・視蓋）、47,769リード/cell（オス・終脳）、41,766リード/cell（オス・視蓋）、1細胞あたりの遺伝子数805（メス・終脳）、484（メス・視蓋）、832（オス・終脳）、646（オス・視蓋）、1細胞あたりの UMIは1,608（メス・終脳）、1,074（メス・視蓋）、1,744（オス・終脳）、1,542（オス・視蓋）UMI/cellのリファレンスデータを取得できた。1細胞あたりの遺伝子数が少ないのは、メダカゲノムデータベースを確認したところ、3’部分配列（polyA付近）が遺伝子モデルに含まれないケースが（３割程度）多いことがわかり、2023年度はゲノムデータベースの整備も必要になることがわかった。

  7. 社会脳ネットワークの全容解明 〜シングルセルトランスクリプトーム解析による高解 像度機能地図の作成〜

    Offer Organization: 公益財団法人 武田科学振興財団

    System: 生命科学研究助成

    Category: 竹内 秀明

    Institution: 東北大学

    2020/04 - 2023/03

  8. 配偶戦略の性スペクトラムを生み出す分子神経基盤の解明

    竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 岡山大学

    2020/04/01 - 2022/03/31

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    メダカにおいてはオキシトシンシステムは配偶戦略の性差が発現するために必須のシステムであり（Yokoi et al 2020 PNAS)、オキシトシンシステムの標的ニューロンの性差を解明すれば、配偶戦略の性差を生み出す分子神経基盤を解明できる可能性がある。本研究ではオキシトシンシステムの標的ニューロン（oxtr1 発現ニューロン）に性差があることを検証することを目的として、 oxtr1 発現ニューロンが存在する終脳/視さく前野と視蓋において scRNA-seq(シングルセルトランスクリプトーム)を行なった。研究開始当初、魚類ではゼブラフィッシュ稚魚でscRNA-seqが実施されているが (Nat Biotechnol 2018) 、成魚脳でscRNA-seqを実施された例がなかった。そこで、本研究ではまずメダカ成体脳を用いて、scRNA-seqの実験系構築を行った。メダカ終脳と視蓋を用いて細胞分離条件の検討を行い、最終的に、メダカ終脳メスから 4,786 細胞を回収し、 1 細胞あたり、 79,738 リード/cell、1,040 遺伝子/cell, 2,198UMI/cell のデータを取得できた。過去のゼブラフィッシュ幼魚の全脳 を用いた報告と比較して、メダカ成魚脳でscRNA-seqの実験系の確立に成功したと判断した。また終脳はクラスタリング解析から 14 種類に分類できることがわかり、oxtr1 発現ニューロンを含む細胞腫を同定した。次に、終脳/視さく前野と視蓋を対象にオスとメスのシングルセルトランスクリプトームを実施した。それぞれの組織から分離した細胞を 10X Genomics Chromium により、分取、バーコーディング、ライブラリー作製することに成功したが、現在、次世代シークエンサーを用いてシーケンスを実施中である。

  9. 魚類「社会脳」の進化的起源と神経 動作原理の解明

    Offer Organization: 公益財団法人 三菱財団

    System: 自然科学研究助成・一般助成

    2020/04 - 2022/03

  10. Study on molecular/neural basis underlying face recognition in medaka fish

    TAKEUCHI Hideaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    2018/04/01 - 2021/03/31

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    Medaka fish have visual ability to discriminate between familiar/unfamiliar mates. We found that the oxytocin signaling pathway (oxt-oxtr1) is required for the mate preference for familiar conspecifics in both sexes. This pathway is essential for eliciting female mate preference for familiar males, while it is also required for the unrestricted and indiscriminate preference of males. Next, we showed that oxtr1 (oxytocin receptor) was expressed in the primary visual center (optic tectum), which is a homolog to the mammalian superior colliculus (SC). Interestingly, primate oxtr is also expressed in the SC and the SC is involved in infant monkeys' rapid and innate face detection. Thus the optic tectum is one of the candidates for brain regions for face detection in medaka fish. In addition, we revealed that visual stimuli from conspecifics activated one brain region (Vd) in the pallium. To identify gene markers for this brain region, we conducted RNA-seq made a gene expression profile.

  11. The basic design of post-hatch development of the pallium and examination of its emergent properties

    TAKEUCHI Hideaki, ISOE Yasuko, KAMEI Yasuhiro, NONAKA Shigenori

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Okayama University

    2016/07/19 - 2019/03/31

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    The telencephalon can be subdivided into the pallium (dorsal part) and subpallium (ventral part). The pallium consists of multiple compartmentalized anatomical regions. In this study, we found that the individual anatomical regions of the pallium comprise multiple clonal units of new-born neurons. Each clonal unit was generated from a single progenitor cell during the post-hatch stage. Here we dissected clonal units (about 30 units), which were genetically labeled with GFP, and compared open chromatin profiles using ATAC-Seq. Here we showed significant difference of profiles between the pallium and subpallium. In addition, individual clonal units have specific profiles, suggesting that epigenetic modification might regulate gene expression of the clonal units and “lineage diversity” could generate compartmentalized structures of the pallium.

  12. Genetic and neural basis of social behaviors in vertebrates

    Naruse Kiyoshi, Takeuchi Hideaki, Yokoi Saori, Fukamachi Shoji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: National Institute for Basic Biology

    2016/04 - 2019/03

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    We have established the KI(c-fos/egr1: DD_mClover3) lines and analyzed the up regulation of DD_mClover3 by TMP(epilepsy inducer). We have detected DD_mClover3 expression 1.5-6 times higher than untreated medaka in KI(c-fos: DD_mClover3) line and 1.9 times higher than untreated medaka in KI(egr1: DD_mClover3) line. These two lines may be useful for visualization of neural networks based on the specific behavior using DD_mClover3 expression. We successfully established KO individuals of the SWS1, SWS2 and LWS genes by CRISPR-Cas9. In addition, we could obtaine SWS1 specific monoclonal antibody.

  13. Clonal analysis in the medaka telencephalon

    Yasuko Isoe, TAKEUCHI Hideaki, NAKAMURA Ryohei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up

    Category: Grant-in-Aid for Research Activity Start-up

    Institution: Okayama University

    2016/08/26 - 2018/03/31

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    The telencephalon is considered as the center of higher brain function. It is constituted by the pallium and sub-pallium. It is reported that the pallium is highly diverse among vertebrates, and the sub-pallium is relatively conserved. It remains unknown how this difference has evolutionally emerged. Since the structure of brain at the developmental stage is similar among species, we focused on the post-hatch neurogenesis and used medaka as an a model. First, we systematically identify clonal units of new-born neurons and found that their morphology were different between the pallium and subpallium. Next, to investigate possible molecular mechanisms, we performed an integrative and genome-wide epigenomic analysis. It is suggested that the expression of genes of the axon guidance cascade were differentially regulated between the pallium and subpallium. It is assumed that different molecular mechanisms are involved in neurogenesis-mediated brain construction in the teleost telencephalon.

  14. 個体記憶が異性の好みを生み出す神経動作原理の解明

    竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 岡山大学

    2016/04/01 - 2018/03/31

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    集団で生活するいくつかの動物は個体認知能力を介して社会適応する。例えば，鳥類では種の90%以上は一夫一婦制を営み，お互いにパートナーを認知している。またいくつかの魚類も個体認知能力を持つ。順位制を持つ熱帯魚の一種（シクリッド）は集団メンバーの順位を記憶して，上位個体からは逃避し，下位個体には接近する。仲間を見分ける上で，ヒトやサルにおいては視覚系が最も重要であり，顔認知に特化した脳領域（顔領域）が大脳皮質に存在する。平成28年度はメダカの社会行動を解析した結果，メダカは個体認知を介して社会に適応していることを発見した。メダカのオスはメスを巡って競争し、メスは長時間そばにいた優位なオスを記憶・識別して性的パートナーとして受け入れる傾向がある。平成29年度はメダカの個体認知の視覚的な手がかりを探索した。体の一部を隠したオスと「お見合い」させることで，メスがオスを識別する際には，「顔」の視覚情報が特に重要であることを見出した。次にプリズムを使って上下逆さまになったオスとお見合いしても識別できないことを示した。さらにメスの識別能力を電気ショック学習実験により検定した。水槽を３つに区切り，中央にメス１匹，両端にオスを１匹ずつ入れ，メスに２匹の異なるオスを視覚的に提示した。 この状態で一方のオスに近づいた時に電気ショックを与える訓練を繰り返すと，メスはそのオスを見分けて避けるようになった。次にプリズムを使ってオスを上下逆さまに提示する と，メスはオスの識別能力は低下した。興味深いことに，メダカオスの代わりに２つの異なる物体を提示した場合，倒立しても物体識別能力は低下しなかった。このことからメダカの顔認知機構は物体認知機構と異なっていることが示唆された。よってメダカ脳においてもヒト同様に顔認知に特化した脳領域（顔領域）が存在する可能性がある。本研究成果は elife に掲載された。

  15. Functional mapping of fish social brain Competitive

    Takeuchi Hideaki, KAMEI Yasuhiro, Isoe Yasuko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    2014/04/01 - 2017/03/31

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    Male medaka fish in a triad relationship (two males and one female) hold a position between the rival male and a female (mate-guarding), which results in improvement of mating success. Behavioral analysis of mutant males revealed that vasotocin system is required for dominance in mate-guarding (PLoS Genetics 2015). Our study also suggested mate-guarding behavior has an effect on visual familiarity and female mate preference. Previously we found that female medaka prefer to mate with visually-familiarized males (Science 2014). We showed that mate-guarding behaviors blocked visual familiarization of a female with the rival in a triad relationship, suggesting that female mate preference for familiar mates may help ascertain social dominance in males（Frontiers in Zoology, 2016).

  16. 社会認知を介した意思決定に関わる神経ネットワークの解明 Competitive

    竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東京大学

    2014/04/01 - 2016/03/31

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    ＜最初期遺伝子を検索・同定＞本年度はメダカの個体記憶を介した配偶者選択に関わる神経ネットーワークを包括的に同定する目的で、メダカ脳で最初期遺伝子を検索・同定した。方法はPTZ（GABA阻害剤）添加によって誘導される癲癇依存に発現が誘導される遺伝子群をマイクロアレイによって検索した。約15,000遺伝子について発現量を比較したところ、18種類の遺伝子が有為に発現上昇することを定量的RT-PCRで確認した。そのうち２つの遺伝子を用いて脳活動のマッピングを行ったところ、メダカメスがオスと長時間見ていた時に活性化している脳領域（終脳背側の一部）を同定した。 ＜脳領域特異的な条件的遺伝子操作法の確立＞これまでに、メダカの条件的遺伝子操作法を確立し、成体脳において細胞系譜単位（単一の神経幹細胞から発生したニューロン群）を遺伝学的にラベルすることに成功している。さらに一部の細胞系譜単位は終脳背側ではブロック化していることを見出している（PLoS One 8:e66597,2013）。本年度は終脳背側の一つの細胞系譜単位が(1)で同定した活性化脳領域に対応することを見出した。 ＜個体記憶を介した行動を定量化する新規行動実験系の確立＞本年度は新規な行動実験系を２つ確立し、以下の２点を示した。(1) メダカの三角関係（オス、オス、メス）において、オスはライバルオスに配偶者防衛行動を示すことで、メスがライバルオスを記憶することを妨害している（Frontier in Zoology in press） 。メダカメスはそばにいたオスを記憶して、配偶者として選択する傾向があるので、メスがライバルオスを選択することを妨害していると考えられる。(2) メスは排卵前には視覚記憶したオスに選択的に近づき行動を示すが、排卵後はオスに対する選択性がなくなる（Zoological Science in press）。

  17. 花粉媒介行動の脳の分子神経基盤とその生態系への影響の解明

    竹内 秀明, 久保 健雄, WANG MU-YUN, WANG Mu-Yun

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    2013/04/26 - 2016/03/31

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    外国人特別研究員のWang博士はマルハナバチのコロニーの中には「注意深い(careful) 個体」と「衝動的な(impulsive) 個体」が存在することを、採餌戦略の違いから明らかにした。注意深い個体は時間をかけて蜜源を選択するが、花蜜量が多く外敵が少ない蜜源を正確に選択する。一方で衝動的な個体は短時間で蜜源を選択するが、質の高い蜜源を正確に選択できないこと(Speed-accuracy tradeoffs)を見出した（Wang et al 2015）。さらに採餌戦略における個体差が脊椎動物にもある否かを確認する目的で、Wang博士はメダカを用いて行動実験系を確立した。メダカは遺伝的背景が同一である近交系が多数、確立・維持されている。そのため近交系ごとに採餌戦略が異なることを示せれば、採餌戦略の違いは遺伝的背景の違いによると考察することができる。先行研究では、メダカに餌を与えた時に視覚刺激を同時に与えると、視覚刺激と餌の情報を連合して学習し、訓練後は視覚刺激依存に近づき行動を示すことがわかっていた（PLoS ONE 8(9): e71685, 2013）。Wang博士は、別の視覚刺激に対して電気ショックを与える実験装置を付加し、報酬と罰の両方の情報が存在する環境で、採餌行動を定量化する行動実験を確立した。Wang博士は５種類の近交系メダカの採餌行動を比較した結果、系統ごとに採餌戦略が異なる傾向があったことから、遺伝学的背景が採餌戦略を規定している可能性が考えられる。

  18. Searching for brain regions involved in individual recognition using medaka fish Competitive

    TAKEUCHI Hideaki, KAMEI Yasuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: The University of Tokyo

    2013/04/01 - 2015/03/31

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    Medaka females can discriminate between socially-familiarized and unfamiliar males and prefer to mate with the former. Here we showed that GnRH3 neurons have a central role in the mate preference for the familiar males. In addition, we established a gene manipulation technique for site-specific Cre/loxP recombination in the telencephalon and a novel behavioral system to investigate an ability of individual recognition.

  19. Analysis of the endocrine and neural mechanisms that synergistically regulate the behavior and physiology of the worker honeybees.

    KUBO Takeo, TAKEUCHI Hideaki, KUNIEDA Takekazu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: The University of Tokyo

    2011/04/01 - 2014/03/31

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    In this study, we aimed to unveil brain neural mechanisms and physiological mechanisms that underlie the honeybee social behaviors. Here, we unveiled novel cellular characteristics of the large-type Kenyon cells (KCs) and identified novel 'middle-type' KCs, both of which compose the mushroom bodies, a higher brain center. The neural activities of the small-type and some 'middle-type' KCs are enhanced in the foragers, whereas those of the class II KCs are enhanced in the Ja@anese worker honeybees that are forming a 'hot defensive bee ball' against the hornets. Furthermore, we showed that the royal jelly that is secreted from young workers (nurse bees) is a cocktail of secretory proteins derived from three exocrine glands and contains cell growth factors that may affect physiological status of the other nest mates.

  20. Study on the molecular/neural basis underlying social behavior in medaka fish Competitive

    TAKEUCHI Hideaki, KAMEI Yasuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: The University of Tokyo

    2011/04/01 - 2014/03/31

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    Medaka females recognize familiar males following prior visual exposure, and prefer to mate with the familiarized males. A subpopulation of gonadotropin-releasing hormone-producing neurons (GnRH3 neurons) is essential for the decision-making switch in the mating choice.

  21. 脊椎度物の社会性を生み出す脳神経基盤と行動法則の解明を目指した生医工連携研究の確立 Competitive

    竹内秀明

    Offer Organization: 基礎生物学研究所

    System: 重点共同利用研究

    2011/04 - 2013/03

  22. Identification of genetic factors that define behavioral differences, and study on their ecological significance for social formation Competitive

    TAKEUCHI Hideaki, NARUSE Kiyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: The University of Tokyo

    2011 - 2012

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    Intraspecific variety of behavioral properties is affected by not only postnatal environments, but also genetic factors. Little attention, however, has been paid on the genetic basis underlying intra-species behavioral difference. Medaka (Oryzias latipes) has high intraspecific genetic variety. We have searched for genetic loci related to behavioral traits, as there are some behavioral differences among medaka strains. Here we presented visual stimuli to two inbred strains, HNI-II and HdrR-II, and compared their responses. As a result, HNI-II showed higher response rate and lower adaptation than HdrR-II. Next, we performed quantitative trait loci (QTL) analysis with F2 individuals between HNI-II and HdrR-II. The result suggested the presence of “un-adaptivity” related loci onchromosome 16.

  23. ヒトの社会を支える脳神経基盤と行動法則の解明を目指した生医工連携研究の確立 Competitive

    竹内秀明

    Offer Organization: 基礎生物学研究所

    System: 重点共同利用研究

    2010/04 - 2011/03

  24. Search for genes and neural circuits involved in medaka interindividual interaction. Competitive

    TAKEUCHI Hideaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: The University of Tokyo

    2009 - 2010

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    We established a behavioral system to induce medaka coordinated movements. Furthermore we established a method for the conditional gene manipulation in the medaka brain and identified some genes encoding neuropeptides as candidate genes involved in medaka social behavior.

  25. 昆虫の社会行動を司る生理機能をモデルとした移動知研究 Competitive

    三浦 徹, 竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特定領域研究

    Category: 特定領域研究

    Institution: 北海道大学

    2008 - 2009

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    シロアリなどの社会性昆虫のコロニーには様々な機能を果たすカーストが存在し,複雑なコミュニケーションに基づく精巧な分業を行うことで社会行動を成立させている.また,メダカに見られるような群れ行動においても個体間の相互作用が重要な役割を果たす.我々は,動物にみられる組織的な行動とその分子基盤を明らかにすることで,生物社会の仕組みと進化を理解し,新たな生命システムを見いだすことを目的として,多角的に研究を進めている.本年度は,シロアリの分業を制御すると考えられるフェロモンの分析を精力的に行い,カースト特異的なタンパクや炭化水素の同定を行った.更に,社会行動の1つである防衛行動に焦点を当て,カーストによる行動の違いを見いだし,状況に応じて可塑的にカーストの行動が制御されていることを明らかとした.さらに,フェロモン分析が進展し,発生のゆらぎにより遺伝子ネットワークが構築されるというモデルの結果も得られた.更に魚の群れ行動についても行動学的および神経生物学的な知見が蓄積された.今後も引き続きそれらの知見や実験系を駆使し,社会行動の総合的な理解につなげたい.更に,包括的な社会システムの理解を目指し,新しい自律分散システムの設計など工学への応用に関しても貢献していきたい.

  26. 分子生物学の新しいモデルとしてのミツバチの開発と利用 （研究担当者） Competitive

    久保健雄

    Offer Organization: 生物系特定産業技術研究支援センター

    System: 生物系特定産業技術研究支援センター新技術・新分野創出のための基礎研究推進事業 平成16年度～20年度

    2004 - 2008

  27. 昆虫の社会行動を統御するための脳分化の解析 Competitive

    三浦 徹, 竹内 秀明, 尾崎 まみこ

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特定領域研究

    Category: 特定領域研究

    Institution: 北海道大学

    2006 - 2007

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    本年度は,昨年度同様,社会性昆虫であるオオシロアリを材料にして,ワーカーとソルジャーの分化に伴う脳機能の分化の解析を行った.行動観察により,カーストによる行動走性の相違を検証し,分子基盤や神経生理の解析を後に行う際の機能マッセイ系が構築された.また,この2つのカースト間において,脳の解剖学的相違,組織切片作成による詳細な細胞学的解析により食道下神経節に存在する大顎筋運動ニューロンが兵隊分化に伴い巨大化することを明らかにした.さらに,行動多型を維持する分子基盤の解明を具体的に推し進めるため,2者間の脳における遺伝子発現の相違をディファレンシャルディスプレイ法などにより検出した.その結果発現量に差のある有力な候補遺伝子がいくつか同定された.現在,遺伝子候補に関して,リアルタイムqPCRなどにより発現動態や発現部位の特定を試みるに至った.また,分担者の尾崎は,クロオオアリを用いて,巣仲間認識に関わる体表炭化水素組成の分析およびこの組成を認識する感覚毛における生理学的解析を進め,脳内の触角葉の糸球体において,感覚毛からの情報が集積されることが明らかとなった.また,分担者の竹内はミツバチのキノコ体特異的に発現する遺伝子の解析を精力的に進めた.その結果キノコ体ではエクダイソンシグナルに関わる遺伝子の発現が分業などにより特異的に変化することが突き止やられた.また行動アッセイ法を確立した.これらの知見を集積し,昆虫の高度な社会性を構築する脳内の分子基盤について詳しい考察がされ,成果を国際誌に投稿した.

  28. 小型魚類の群れ行動に関わる脳領域の同定と分子的基盤の解明 Competitive

    竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽研究

    Category: 萌芽研究

    Institution: 東京大学

    2006 - 2007

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    多くの魚は自然条件下では水流の中で周りの景色に定位するために、視野を一定に保とうと運動する性質がある(視運動反応)。視運動反応は研究室内で簡単に誘導することができる。方法は固定した円形水槽に魚を入れ、水槽外側で縦縞模様が描かれた円柱をモータで回転させると、水槽内の魚は周りの背景に定位するために縦縞を追いかけて回転運動をする。1972年にPartridgeらは大型水槽(直径10m)でタラなどの海水魚の魚群(20匹)を用いて視運動反応を誘導すると協調的に集団遊泳することを示している。本年度は小型水槽(直径15cm)で2個体のメダカを用いて視運動反応を同時に誘導すると初期状態によらず個体間距離が常に一定(2〜4cm)に保って遊泳することを発見した。また小型淡水フグ(体長3cm)でも同様の結果が得られ、本実験系は様々な魚類で用いることができることが分かった。また淡水フグとメダカの間では協調的行動が生じなかったことから、この協調的行動は同種個体間認識を介していることも示された(論文準備中)。本研究では申請者が独自に開発した実験系と分子遺伝学が駆使できるメダカを用いる事で魚類の協調的行動の脳の分子及び神経基盤を世界に先駆けて解明できることが期待される

  29. ミツバチの脳のキノコ体に特異的に発現するM5遺伝子の解析

    竹内 秀明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東京大学

    1998 - 1999

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