In Situ Electrochemical Measurement of Alkaline Phosphatase Activity in Engineered Gut Models Using a Porous Membrane Electrode Device International-journal Peer-reviewed

Yoshinobu Utagawa, Takeo Miyake, Yasuhiko Shinoda, Masateru Yamazaki, Hiroya Abe, Hitoshi Shiku, Kosuke Ino

Lab on a Chip　2025

DOI： 10.1039/D5LC00341E 　

Electrochemiluminescence microscopy for the investigation of peptide interactions within planar lipid membranes International-journal Invited Peer-reviewed

Kaoru Hiramoto, Kosuke Ino, Ibuki Takahashi, Ayumi Hirano-Iwata, Hitoshi Shiku

Faraday Discussions　257　137-150　2025

DOI： 10.1039/d4fd00137k 　

ISSN： 1359-6640

eISSN： 1364-5498

Extended spherical diffusion theory: Electrochemiluminescence imaging analysis of diffusive molecules from spherical biosamples International-journal Peer-reviewed

Kosuke Ino, Miyu Mashiko, Yusuke Kanno, Yeyi Tang, Shuzo Masui, Takasi Nisisako, Kaoru Hiramoto, Hiroya Abe, Hitoshi Shiku

Analytical Chemistry　96　(48)　18967-18976　2024/11/19

DOI： 10.1021/acs.analchem.4c03167 　

ISSN： 0003-2700

eISSN： 1520-6882

Electrochemical permeability assays of hydrolyzed acetylsalicylic acid (aspirin) in engineered gut models International-journal Peer-reviewed

Chisato Ito, Kosuke Ino, Yoshinobu Utagawa, Kazuyuki Iwase, Yasuhiko Shinoda, Hiroya Abe, Hitoshi Shiku

Chemistry Letters　53　(9)　upae174　2024/08/30

DOI： 10.1093/chemle/upae174 　

ISSN： 0366-7022

eISSN： 1348-0715

Fabrication of two-layer microfluidic devices with porous electrodes using printed sacrificial layers International-journal Peer-reviewed

Kosuke Ino, An Konno, Yoshinobu Utagawa, Taiyo Kanno, Kazuyuki Iwase, Hiroya Abe, Hitoshi Shiku

Micromachines　15　(8)　1054-1054　2024/08/22

DOI： 10.3390/mi15081054 　

eISSN： 2072-666X

Enzyme-free in-situ electrochemical measurement using a porous membrane electrode for glucose transport into cell spheroids International-journal Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Yasuhiko Shinoda, Masateru Yamazaki, Hiroya Abe, Hitoshi Shiku

ACS Sensors　9　(8)　4248-4255　2024/07

DOI： 10.1021/acssensors.4c01230 　

Scanning electrochemical microscopy for determining oxygen consumption rates of cells in hydrogel fibers fabricated using an extrusion 3D bioprinter International-journal Peer-reviewed

Kosuke Ino, Mana Wachi, Yoshinobu Utagawa, An Konno, Masahiro Takinoue, Hiroya Abe, Hitoshi Shiku

Analytica Chimica Acta　1304　342539-342539　2024/05

DOI： 10.1016/j.aca.2024.342539 　

ISSN： 0003-2670

Osteogenic differentiation of mesenchymal stem cell spheroids: A microfluidic device and electrochemiluminescence imaging study International-journal Invited Peer-reviewed

Kaoru Hiramoto, An Konno, Yuji Nashimoto, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku

Electrochimica Acta　144291-144291　2024/04

DOI： 10.1016/j.electacta.2024.144291 　

ISSN： 0013-4686

Comprehensive cell adhesion analysis using electrochemiluminescence imaging and electrochemical impedance spectroscopy International-journal Invited Peer-reviewed

Kimiharu Oba, Kosuke Ino, Yoshinobu Utagawa, Hiroya Abe, Hitoshi Shiku

Electrochemistry　92　(2)　022009　2024/02

DOI： 10.5796/electrochemistry.23-68109 　

ISSN： 1344-3542

eISSN： 2186-2451

Microfluidic vascular formation model for assessing angiogenic capacities of single islets International-journal Peer-reviewed

Yuji Nashimoto, An Konno, Takuto Imaizumi, Kaori Nishikawa, Kosuke Ino, Takeshi Hori, Hirokazu Kaji, Hirofumi Shintaku, Masafumi Goto, Hitoshi Shiku

Biotechnology and Bioengineering　121　(3)　1050-1059　2024

DOI： 10.1002/bit.28631 　

ISSN： 0006-3592

eISSN： 1097-0290

Vasculature-on-a-chip with a porous membrane electrode for in situ electrochemical detection of nitric oxide released from endothelial cells International-journal Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Kazuyuki Iwase, Yuji Nashimoto, Itaru Honma, Hitoshi Shiku

Analytical Chemistry　95　(49)　18158-18165　2023/11/28

DOI： 10.1021/acs.analchem.3c03684 　

ISSN： 0003-2700

eISSN： 1520-6882

Effect of valinomycin on the respiration activity of zebrafish embryos using a large-scale-integration-based multiple amperometric biosensor International-journal Peer-reviewed

Kazuki Terao, Masato Suzuki, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Tomoyuki Yasukawa

Sensors and Materials　35　(10)　4781-4781　2023/10/25

DOI： 10.18494/sam4493 　

ISSN： 0914-4935

eISSN： 2435-0869

Fabrication and cell culture applications of core‐shell hydrogel fibers composed of chitosan/DNA interfacial polyelectrolyte complexation and calcium alginate: straight and beaded core variations International-journal Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Masahiro Takinoue, Hitoshi Shiku

Advanced Healthcare Materials　12　(31)　2302011　2023/08/03

DOI： 10.1002/adhm.202302011 　

ISSN： 2192-2640

eISSN： 2192-2659

Evaluation of respiratory and secretory activities of multicellular spheroids via electrochemiluminescence imaging International-journal Invited Peer-reviewed

Kaoru Hiramoto, Keika Komatsu, Ryota Shikuwa, An Konno, Yusuke Sato, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku

Electrochimica Acta　458　142507　2023/08

DOI： 10.1016/j.electacta.2023.142507 　

Topographical evaluation of human mesenchymal stem cells during osteogenic differentiation using scanning ion conductance microscopy International-journal Peer-reviewed

Kota Nozawa, Xuyang Zhang, Takuo Nakamura, Yuji Nashimoto, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku

Electrochimica Acta　449　142192　2023/05

DOI： 10.1016/j.electacta.2023.142192 　

Simple, rapid, and large‐scale fabrication of multi‐branched hydrogels based on viscous fingering for cell culture applications International-journal Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Hitoshi Shiku

Macromolecular Bioscience　23　(9)　2300069　2023/04/22

DOI： 10.1002/mabi.202300069 　

ISSN： 1616-5187

eISSN： 1616-5195

Effects of ion concentration on ion current rectification using a glass nanocapillary Peer-reviewed

Chisato Ito, Kosuke Ino, Kaoru Hiramoto, Yuji Nashimoto, Hitoshi Shiku

Bunseki Kagaku　72　(3)　117-123　2023/03

DOI： 10.2116/bunsekikagaku.72.117 　

Droplet-free digital immunoassay based on electrochemiluminescence Peer-reviewed

Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku

Biosensors and Bioelectronics: X　100312　2023/02

DOI： 10.1016/j.biosx.2023.100312 　

ISSN： 2590-1370

Oxygen metabolism analysis of a single organoid toward the noninvasive discrimination of cancer subpopulations with different growth capabilities International-journal Peer-reviewed

Yuji Nashimoto, Shotaro Shishido, Kunishige Onuma, Kosuke Ino, Masahiro Inoue, Hitoshi Shiku

Frontiers in Bioengineering and Biotechnology　11　1184325-1184325　2023

DOI： 10.3389/fbioe.2023.1184325 　

Highly sensitive electrochemical endotoxin sensor based on redox cycling using an interdigitated array electrode device Peer-reviewed

Kentaro Ito, Kumi Y. Inoue, Takahiro Ito-Sasaki, Miho Ikegawa, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku

Micromachines　14　(2)　327　2023/01

DOI： 10.3390/mi14020327 　

Electrochemical sensing of oxygen metabolism for a three-dimensional cultured model with biomimetic vascular flow International-journal Peer-reviewed

Yuji Nashimoto, Rei Mukomoto, Takuto Imaizumi, Takato Terai, Shotaro Shishido, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Kunishige Onuma, Masahiro Inoue, Hitoshi Shiku

Biosensors and Bioelectronics　219　114808-114808　2023/01

DOI： 10.1016/j.bios.2022.114808 　

ISSN： 0956-5663

High-sensitivity amperometric dual immunoassay using two cascade reactions with signal amplification of redox cycling in nanoscale gap International-journal Peer-reviewed

Kentaro Ito, Kumi Y Inoue, Kosuke Ino, Hitoshi Shiku

Analytical Chemistry　94　(47)　16451-16460　2022/11/04

DOI： 10.1021/acs.analchem.2c03921 　

ISSN： 0003-2700

eISSN： 1520-6882

Highly sensitive electrochemical immunoassay using signal amplification of the coagulation cascade International-journal Peer-reviewed

Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku

Analytical Chemistry　94　(36)　12427-12434　2022/08/26

DOI： 10.1021/acs.analchem.2c02241 　

ISSN： 0003-2700

eISSN： 1520-6882

Electrochemical microwell sensor with Fe-N co-doped carbon catalyst to monitor nitric oxide release from endothelial cell spheroids International-journal Peer-reviewed

Kaoru Hiramoto, Kazuyuki Iwase, Yoshinobu Utagawa, Yuji Nashimoto, Itaru Honma, Kosuke Ino, Hitoshi Shiku

Analytical Sciences　38　1297-1304　2022/07

DOI： 10.1007/s44211-022-00160-0 　

ISSN： 0910-6340

eISSN： 1348-2246

Electrochemiluminescence imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds International-journal Peer-reviewed

Kosuke Ino, Keika Komatsu, Kaoru Hiramoto, Yoshinobu Utagawa, Yuji Nashimoto, Hitoshi Shiku

Electrochimica Acta　415　140240　2022/03

DOI： 10.1016/j.electacta.2022.140240 　

Electrochemical glue for binding chitosan/alginate hydrogel fibers for cell culture International-journal Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Tatsuki Kumagai, Kaoru Hiramoto, Masahiro Takinoue, Yuji Nashimoto, Hitoshi Shiku

Micromachines　13　(3)　420-420　2022/03

DOI： 10.3390/mi13030420 　

Electrochemiluminescence imaging based on bipolar electrochemistry using commercially available anisotropic conductive films International-journal Peer-reviewed

Rise Akasaka, Kosuke Ino, Tomoki Iwama, Kumi Y. Inoue, Yuji Nashimoto, Hitoshi Shiku

Sensors and Materials　34　(8)　3113-3122　2022/01

DOI： 10.18494/SAM3745 　

Ion conductance-based perfusability assay of vascular vessel models in microfluidic devices International-journal Peer-reviewed

Rise Akasaka, Masashi Ozawa, Yuji Nashimoto, Kosuke Ino, Hitoshi Shiku

Micromachines　12　(12)　1491　2021/12

DOI： 10.3390/mi12121491 　

Electrochemical imaging of endothelial permeability using a large-scale integration-based device International-journal Peer-reviewed

Kosuke Ino, Hao-Jen Pai, Kaoru Hiramoto, Yoshinobu Utagawa, Yuji Nashimoto, Hitoshi Shiku

ACS Omega　6　(51)　35476-35483　2021/12

DOI： 10.1021/acsomega.1c04931 　

Electrochemical immunoassay with dual-signal amplification for redox cycling within a nanoscale gap International-journal International-coauthorship Peer-reviewed

Kentaro Ito, Kumi Y. Inoue, Takahiro Ito-Sasaki, Kosuke Ino, Hitoshi Shiku

ACS Applied Nano Materials　4　(11)　12393-12400　2021/10/22

DOI： 10.1021/acsanm.1c02837 　

ISSN： 2574-0970

eISSN： 2574-0970

Simultaneous monitoring of oxygen consumption and movement of zebrafish embryos based on an LSI-based electrochemical multiple-biosensor Peer-reviewed

Masato Suzuki, Yuka Iwaki, Kazuki Terao, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Tomoyuki Yasukawa

Bunseki Kagaku　70　(9)　535-540　2021/09

Topography and permeability analyses of vasculature-on-a-chip using scanning probe microscopies International-journal

Yuji Nashimoto, Minori Abe, Ryota Fujii, Noriko Taira, Hiroki Ida, Yasufumi Takahashi, Kosuke Ino, Javier Ramon-Azcon, Hitoshi Shiku

Advanced Healthcare Materials　e2101186　2021/08/19

DOI： 10.1002/adhm.202101186 　

Electrochemiluminescence imaging of respiratory activity of cellular spheroids using sequential potential steps International-journal Peer-reviewed

Kaoru Hiramoto, Kosuke Ino, Keika Komatsu, Yuji Nashimoto, Hitoshi Shiku

Biosensors and Bioelectronics　181　113123-113123　2021/06/01

DOI： 10.1016/j.bios.2021.113123 　

Micropipette-based navigation in a microvascular model for imaging endothelial cell topography using scanning ion conductance microscopy Peer-reviewed

Taira, Noriko, Nashimoto, Yuji, Ino, Kosuke, Ida, Hiroki, Imaizumi, Takuto, Kumatani, Akichika, Takahashi, Yasufumi, Shiku, Hitoshi

Analytical Chemistry　93　(11)　4902-4908　2021/03

DOI： 10.1021/acs.analchem.0c05174 　

Droplet array device for electrochemical detection of methylene blue based on local redox cycling Peer-reviewed

Kaoru Hiramoto, Keika Komatsu, Yuta Yamada, Yuji Nashimoto, Tomokazu Matsue, Kosuke Ino, Hitoshi Shiku

Bunseki Kagaku　70　(3)　183-189　2021/03

Electrodeposition of thiolated polymer-based hydrogels via disulfide formation using electrogenerated benzoquinone Peer-reviewed

Kosuke Ino, Ayako Tamura, Kaoru Hiramoto, Mika T. Fukuda, Yuji Nashimoto, Hitoshi Shiku

Chemistry Letters　50　(2)　256-259　2021/02/05

DOI： 10.1246/cl.200732 　

ISSN： 0366-7022

eISSN： 1348-0715

Fabrication of three-dimensional calcium alginate hydrogels using sacrificial templates of sugar Peer-reviewed

Kosuke Ino, Mika T Fukuda, Kaoru Hiramoto, Noriko Taira, Yuji Nashimoto, Hitoshi Shiku

Journal of Bioscience and Bioengineering　130　(5)　539-544　2020/08/02

DOI： 10.1016/j.jbiosc.2020.06.014 　

Oxygen consumption rate of tumour spheroids during necrotic-like core formation International-journal Peer-reviewed

Rei Mukomoto, Yuji Nashimoto, Takato Terai, Takuto Imaizumi, Kaoru Hiramoto, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Hitoshi Shiku

Analyst　145　6342-6348　2020/07

DOI： 10.1039/d0an00979b 　

ISSN： 0003-2654

eISSN： 1364-5528

Electrochemical measurement of respiratory activity for evaluation of fibroblast spheroids containing endothelial cell networks International-journal Invited Peer-reviewed

Kaoru Hiramoto, Hao-Jen Pai, Kosuke Ino, Yuji Nashimoto, Hitoshi Shiku

Electrochimica Acta　340　135979　2020/04

DOI： 10.1016/j.electacta.2020.135979 　

Closed Bipolar Electrode Array for On-Chip Analysis of Cellular Respiration by Cell Aggregates International-journal Peer-reviewed

Kosuke Ino, Ryosuke Yaegaki, Kaoru Hiramoto, Yuji Nashimoto, Hitoshi Shiku

ACS Sensors　5　(3)　740-745　2020/03/27

DOI： 10.1021/acssensors.9b02061 　

ISSN： 2379-3694

eISSN： 2379-3694

Real-time imaging of photosynthetic oxygen evolution from spinach using LSI-based biosensor Peer-reviewed

Shigenobu Kasai, Yamato Sugiura, Ankush Prasad, Kumi Y. Inoue, Teruya Sato, Tomohiro Honmo, Aditya Kumar, Pavel Pospíšil, Kosuke Ino, Yuka Hashi, Yoko Furubayashi, Masahki Matsudaira, Atsushi Suda, Ryota Kunikata, Tomokazu Matsue

Scientific Reports　9　12234　2019/08

DOI： 10.1038/s41598-019-48561-y 　

ISSN： 2045-2322

Genipin crosslinking of electrodeposited chitosan/gelatin hydrogels for cell culture Peer-reviewed

Ayako Tamura, Kaoru Hiramoto, Kosuke Ino, Noriko Taira, Yuji Nashimoto, Hitoshi Shiku

Chemistry Letters　48　(10)　1178-1180　2019/07

DOI： 10.1246/cl.190466 　

ISSN： 0366-7022 1348-0715

Site-specific cytosol sampling from a single cell in an intact tumor spheroid using an electrochemical syringe Peer-reviewed

Yuji Nashimoto, Masakuni Echigo, Kosuke Ino, Hitoshi Shiku

Analytical Chemistry　91　(14)　8772-8776　2019/06

DOI： 10.1021/acs.analchem.9b02062 　

Electrodeposition-based rapid bioprinting of 3D-designed hydrogels with a pin art device Peer-reviewed

Noriko Taira, Kosuke Ino, Hiroki Ida, Yuji Nashimoto, Hitoshi Shiku

Biofabrication　11　(3)　035018-035018　2019/05

DOI： 10.1088/1758-5090/ab166e 　

Electrochemical fabrication of fibrin gels via cascade reaction for cell culture Peer-reviewed

Noriko Taira, Kosuke Ino, Tatsuki Kumagai, Yuji Nashimoto, Hitoshi Shiku

Chemical Communications　55　5335-5338　2019/05

DOI： 10.1039/C9CC01576K 　

A highly sensitive endotoxin sensor based on redox cycling in a nanocavity Peer-reviewed

Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

Analyst　144　(11)　3659-3667　2019/04

DOI： 10.1039/C9AN00478E 　

ISSN： 0003-2654

eISSN： 1364-5528

Combination of double-mediator system with large-scale integration-based amperometric devices for detecting NAD(P)H:quinone oxidoreductase 1 activity of cancer cell aggregates Peer-reviewed

Kosuke Ino, Takehiro Onodera, Mika T. Fukuda, Yuji Nashimoto, Hitoshi Shiku

ACS Sensors　4　(6)　1619-1625　2019/04

DOI： 10.1021/acssensors.9b00344 　

Electrochemical imaging of cell activity in hydrogels embedded in grid-shaped polycaprolactone scaffolds using a large-scale integration (LSI)-based amperometric device Invited Peer-reviewed

Kosuke Ino, Yuki Yokokawa, Noriko Taira, Atsushi Suda, Ryota Kunikata, Yuji Nashimoto, Tomokazu Matsue, Hitoshi Shiku

Analytical Sciences　35　(1)　39-43　2019/01

DOI： 10.2116/analsci.18SDP01 　

ISSN： 0910-6340 1348-2246

Differential electrochemicolor imaging using LSI-based device for simultaneous detection of multiple analytes Invited Peer-reviewed

Kosuke Ino, Takehiro Onodera, Yuji Nashimoto, Hitoshi Shiku

Sensors and Materials　31　(1)　13-22　2019/01

DOI： 10.18494/SAM.2019.2035 　

ISSN： 0914-4935

Simultaneous and selective imaging of dopamine and glutamate using an enzyme-modified large-scale integration (LSI)-based amperometric electrochemical device Peer-reviewed

Hiroya Abe, Tomoki Iwama, Hiroshi Yabu, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Tomokazu Matsue

Electroanalysis　30　2841-2846　2018/09

DOI： 10.1002/elan.201800386 　

Electrochemical printing of calcium alginate/gelatin hydrogel Peer-reviewed

Noriko Taira, Kosuke Ino, Jordan Robert, Hitoshi Shiku

Electrochimica Acta　281　429-436　2018/08/10

DOI： 10.1016/j.electacta.2018.05.124 　

ISSN： 0013-4686

Local hydrogel fabrication based on electrodeposition with a large-scale integration (LSI)-based amperometric device Peer-reviewed

Kosuke Ino, Mayuko Terauchi, Mai Gakumasawa, Noriko Taira, Atsushi Suda, Ryota Kunikata, Tomokazu Matsue, Hitoshi Shiku

Sensors and Actuators B: Chemical　277　95-101　2018/08

DOI： 10.1016/j.snb.2018.08.135 　

Hydrogel electrodeposition based on bipolar electrochemistry Peer-reviewed

Kosuke Ino, Tomoaki Matsumoto, Noriko Taira, Tatsuki Kumagai, Yuji Nashimoto, Hitoshi Shiku

Lab on a Chip　18　2425-2432　2018/06

DOI： 10.1039/C8LC00465J 　

Electrochemicolor imaging of endogenous alkaline phosphatase and respiratory activities of mesenchymal stem cell aggregates in early-stage osteodifferentiation Invited Peer-reviewed

Kosuke Ino, Takehiro Onodera, Yusuke Kanno, Atsushi Suda, Ryota Kunikata, Tomokazu Matsue, Hitoshi Shiku

Electrochimica Acta　268　554-561　2018/04/01

DOI： 10.1016/j.electacta.2018.02.094 　

ISSN： 0013-4686

Micropatterning of nafion membranes on an electrode array using photolithographic and lift-off techniques for selective electrochemical detection and signal accumulation Peer-reviewed

Mayuko Terauchi, Kosuke Ino, Yusuke Kanno, Shunsuke Imai, Hitoshi Shiku, Tomokazu Matsue

Chemistry Letters　47　(2)　204-206　2018

DOI： 10.1246/cl.171031 　

ISSN： 1348-0715 0366-7022

Electrochemicolor Imaging Using an LSI-Based Device for Multiplexed Cell Assays Peer-reviewed

Yusuke Kanno, Kosuke Ino, Hiroya Abe, Chika Sakamoto, Takehiro Onodera, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　89　(23)　12778-12786　2017/12

DOI： 10.1021/acs.analchem.7b03042 　

ISSN： 0003-2700

eISSN： 1520-6882

Development of Oxygen Consumption Analysis with an on-Chip Electrochemical Device and Simulation Peer-reviewed

Kaoru Hiramoto, Masahiro Yasumi, Hiroshi Ushio, Atsushi Shunori, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　89　(19)　10303-10310　2017/10

DOI： 10.1021/acs.analchem.7b02074 　

ISSN： 0003-2700

eISSN： 1520-6882

Electrochemical Motion Tracking of Microorganisms Using a Large-Scale-Integration-Based Amperometric Device Peer-reviewed

Kosuke Ino, Yusuke Kanno, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　56　(24)　6818-6822　2017/06

DOI： 10.1002/anie.201701541 　

ISSN： 1433-7851

eISSN： 1521-3773

Cell Sheet Fabrication Using RGD Peptide-coupled Alginate Hydrogels Fabricated by an Electrodeposition Method Peer-reviewed

Fumisato Ozawa, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

CHEMISTRY LETTERS　46　(4)　605-608　2017/04

DOI： 10.1246/cl.170003 　

ISSN： 0366-7022

eISSN： 1348-0715

Binary-number-based digital electrochemical detection using a single working electrode with multiple sensors Peer-reviewed

Kosuke Ino, Yusuke Kanno, Yuta Yamada, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY COMMUNICATIONS　77　76-80　2017/04

DOI： 10.1016/j.elecom.2017.02.016 　

ISSN： 1388-2481

eISSN： 1873-1902

Amperometric detection of apoptosis by using p-methoxyaniline-conjugated substrate for caspase-3 Peer-reviewed

Sun Sixiang, Kumi Y. Inoue, Shusaku Shiomoto, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ChemElectroChem　4　(4)　941-946　2017/03/16

DOI： 10.1002/celc.201600700 　

Continuous collection and simultaneous detection of picoliter volume of nucleic acid samples using a mille-feuille probe Peer-reviewed

Hidenori Ito, Motoki Tanaka, Yuanshu Zhou, Yuji Nashimoto, Yasufumi Takahashi, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

Analytical and Bioanalytical Chemistry　409　(4)　961-969　2017/02

DOI： 10.1007/s00216-016-0006-y 　

Molecular electrochemical switching element based on diffusive molecular competition for multipoint electrochemical detection of respiration activity of cell aggregates Peer-reviewed

Kosuke Ino, Yuta Yamada, Yusuke Kanno, Shunsuke Imai, Hitoshi Shiku, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　234　201-208　2016/10

DOI： 10.1016/j.snb.2016.04.160 　

ISSN： 0925-4005

A new in vitro co-culture model using magnetic force-based nanotechnology Peer-reviewed

Hiroki Takanari, Keiko Miwa, XianMing Fu, Junichi Nakai, Akira Ito, Kousuke Ino, Hiroyuki Honda, Wataru Tonomura, Satoshi Konishi, Tobias Opthof, Marcel AG van, der Heyden, Itsuo Kodama, Jong-Kook

Journal of Cellular Physiology　231　(10)　2249-2256　2016/10

DOI： 10.1002/jcp.25342 　

Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture Peer-reviewed

Fumisato Ozawa, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

MATERIALS　9　(9)　744　2016/09

DOI： 10.3390/ma9090744 　

ISSN： 1996-1944

Evaluation of mRNA localization using double barrel scanning ion conductance microscopy Peer-reviewed

Yuji Nashimoto, Yasufumi Takahashi, Yuanshu Zhou, Hidenori Ito, Hiroki Ida, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

ACS Nano　10　(7)　6915-6922　2016/07/26

DOI： 10.1021/acsnano.6b02753 　

Imaging of enzyme activity using bio-LSI system enables simultaneous immunosensing of different analytes in multiple specimens Peer-reviewed

Toshiki Hokuto, Tomoyuki Yasukawa, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Fumio Mizutani

Biotechnology Journal　11　(6)　838-842　2016/06

DOI： 10.1002/biot.201500559 　

Sequential monitoring of oxygen consumption rate of mouse embryo bodies in glucose-depleted solution Peer-reviewed

Hitoshi Shiku, Nana Aoki, Toshiharu Arai, Yuanshu Zhou, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue

Electrochemistry　84　(5)　302-304　2016/05/05

DOI： 10.5796/electrochemistry.84.302 　

Redox Cycling-based Electrochemical Reporter Gene Assay for Single Cells Using a Scanning Electrochemical Microscope-microwell System Peer-reviewed

Hiroki Ida, Kosuke Ino, Junya Suzuki, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY　84　(5)　308-311　2016/05

DOI： 10.5796/electrochemistry.84.308 　

ISSN： 1344-3542

Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device Peer-reviewed

Hiroya Abe, Yusuke Kanno, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY　84　(5)　364-367　2016/05

DOI： 10.5796/electrochemistry.84.364 　

ISSN： 1344-3542

Potentiometric bioimaging with a large-scale integration (LSI)-based electrochemical device for detection of enzyme activity Peer-reviewed

Yusuke Kanno, Kosuke Ino, Chika Sakamoto, Kumi Y. Inoue, Masahki Matsudaira, Atsushi Suda, Ryota Kunikata, Tomohiro Ishikawa, Hiroya Abe, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　77　709-714　2016/03

DOI： 10.1016/j.bios.2015.10.021 　

ISSN： 0956-5663

eISSN： 1873-4235

Liquid-junction-free system for substitutional stripping voltammetry using a closed bipolar electrode system Peer-reviewed

Shinichiro Takano, Kumi Y. Inoue, Miho Ikegawa, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Electrochemistry Communications　66　34-37　2016/02/27

DOI： 10.1016/j.elecom.2016.02.014 　

Localized gene expression analysis during sprouting angiogenesis in mouse embryoid bodies using a double barrel carbon probe Peer-reviewed

Hidenori Ito, Yuji Nashimoto, Yuanshu Zhou, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Analytical Chemistry　88　(1)　610-613　2016/01/05

DOI： 10.1021/acs.analchem.5b04338 　

Evaluation of senescence in individual MCF-7 spheroids based on electrochemical measurement of senescence-associated β-galactosidase activity Peer-reviewed

Yuanshu Zhou, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Electrochimica Acta　186　449-454　2015/12/20

DOI： 10.1016/j.electacta.2015.10.115 　

Metabolic suppression during mesodermal differentiation of embryonic stem cells identified by single-cell comprehensive gene expression analysis Peer-reviewed

Yuanshu Zhou, Ikuma Fujisawa, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

Molecular BioSystems　11　(9)　2560-2567　2015/07/13

DOI： 10.1039/C5MB00340G 　

Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity Peer-reviewed

Yusuke Kanno, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL SCIENCES　31　(7)　715-719　2015/07

DOI： 10.2116/analsci.31.715 　

ISSN： 0910-6340

eISSN： 1348-2246

Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors Peer-reviewed

Hiroya Abe, Kosuke Ino, Chen-Zhong Li, Yusuke Kanno, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　87　(12)　6364-6370　2015/06

DOI： 10.1021/acs.analchem.5b01307 　

ISSN： 0003-2700

eISSN： 1520-6882

Feedback mode-based electrochemical imaging of conductivity and topography for large substrate surfaces using an LSI-based amperometric chip device with 400 sensors Peer-reviewed

Yusuke Kanno, Kosuke Ino, Kumi Y. Inoue, Mustafa Sen, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hiroya Abe, Chen-Zhong Li, Hitoshi Shiku, Tomokazu Matsue

JOURNAL OF ELECTROANALYTICAL CHEMISTRY　741　109-113　2015/03

DOI： 10.1016/j.jelechem.2015.01.020 　

ISSN： 1572-6657

eISSN： 1873-2569

Improving the electrochemical imaging sensitivity of scanning electrochemical microscopy-scanning ion conductance microscopy by using electrochemical Pt deposition Peer-reviewed

Mustafa Şen, Yasufumi Takahashi, Yoshiharu Matsumae, Yoshiko Horiguchi, Akichika Kumatani, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Analytical Chemistry　87　(6)　3484-3489　2015/02/09

DOI： 10.1021/acs.analchem.5b00027 　

Nanoscale imaging of an unlabeled secretory protein in living cells using scanning ion conductance microscopy Peer-reviewed

Yuji Nashimoto, Yasufumi Takahashi, Hiroki Ida, Yoshiharu Matsumae, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Analytical Chemistry　87　(5)　2542-2545　2015/02/03

DOI： 10.1021/ac5046388 　

Advanced LSI-based amperometric sensor array with light-shielding structure for effective removal of photocurrent and mode selectable function for individual operation of 400 electrodes Peer-reviewed

Kumi Y. Inoue, Masahki Matsudaira, Masanori Nakano, Kosuke Ino, Chika Sakamoto, Yusuke Kanno, Reyushi Kubo, Ryota Kunikata, Atsushi Kira, Atsushi Suda, Ryota Tsurumi, Toshihito Shioya, Shinya Yoshida, Masanori Muroyama, Tomohiro Ishikawa, Hitoshi Shiku, Shiro Satoh, Masayoshi Esashi, Tomokazu Matsue

Lab on a Chip　15　(3)　848-856　2015/01/22

DOI： 10.1039/C4LC01099J 　

A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies Peer-reviewed

Yusuke Kanno, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　15　(23)　4404-4414　2015

DOI： 10.1039/c5lc01016k 　

ISSN： 1473-0197

eISSN： 1473-0189

Nanoscale visualization of redox activity at lithium-ion battery cathodes Peer-reviewed

Yasufumi Takahashi, Akichika Kumatani, Hirokazu Munakata, Hirotaka Inomata, Komachi Ito, Kosuke Ino, Hitoshi Shiku, Patrick R. Unwin, Yuri E. Korchev, Kiyoshi Kanamura, Tomokazu Matsue

Nature Communications　5　5450　2014/11/17

DOI： 10.1038/ncomms6450 　

Electrochemical sensor with substitutional stripping voltammetry for highly sensitive endotoxin assay Peer-reviewed

Shinichiro Takano, Kumi Y. Inoue, Satoko Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Analyst　139　(19)　5001-5006　2014/10/07

DOI： 10.1039/C4AN00510D 　

Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique Peer-reviewed

Ahadian, S., Yamada, S., Ram{\'o}n-Azc{\'o}n, J., Ino, K., Shiku, H., Khademhosseini, A., Matsue, T.

Lab on a Chip　14　(19)　3690-4-3694　2014/10/07

DOI： 10.1039/C4LC00479E 　

Electrochemical monitoring of intracellular enzyme activity of single living mammalian cells by using a double-mediator system Peer-reviewed

Yoshiharu Matsumae, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Analytica Chimica Acta　842　20-26　2014/09/09

DOI： 10.1016/j.aca.2014.06.047 　

Electrochemical evaluation of sarcomeric alpha-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array Peer-reviewed

Mustafa Sen, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL METHODS　6　(16)　6337-6342　2014/08

DOI： 10.1039/c4ay00791c 　

ISSN： 1759-9660

eISSN： 1759-9679

Immuno nanoparticles integrated electrical control of targeted cancer cell development using whole cell bioelectronic device Peer-reviewed

Evangelia Hondroulis, Rui Zhang, Chengxiao Zhang, Chunying Chen, Kosuke Ino, Tomokazu Matsue, Chen-Zhong Li

Theranostics　4　(9)　919-930　2014/07/13

DOI： 10.7150/thno.8575 　

Electrochemical approach for the development of a simple method for detecting cell apoptosis based on caspase-3 activity Peer-reviewed

Shinichiro Takano, Shusaku Shiomoto, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Analytical Chemistry　86　(10)　4723-8-4728　2014/05/20

DOI： 10.1021/ac403394z 　

Nanoscale cell surface topography imaging using scanning ion conductance microscopy Peer-reviewed

Yasufumi Takahashi, Komachi Ito, Xiongwei Wang, Yoshiharu Matsumae, Hirokazu Komaki, Akichika Kumatani, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Electrochemistry　82　(5)　331-334　2014/05/05

DOI： 10.5796/electrochemistry.82.331 　

Densified Electrochemical Sensors Based on Local Redox Cycling between Vertically Separated Electrodes in Substrate Generation/Chip Collection and Extended Feedback Modes Peer-reviewed

Kosuke Ino, Yusuke Kanno, Taku Nishijo, Hirokazu Komaki, Yuta Yamada, Shinya Yoshida, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　86　(8)　4016-4023　2014/04

DOI： 10.1021/ac500435d 　

ISSN： 0003-2700

eISSN： 1520-6882

SU-8-based Flexible Amperometric Device with IDA Electrodes to Regenerate Redox Species in Small Spaces Peer-reviewed

Yusuke Kanno, Takehito Goto, Kosuke Ino, Kumi Y. Inoue, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL SCIENCES　30　(2)　305-309　2014/02

DOI： 10.2116/analsci.30.305 　

ISSN： 0910-6340

eISSN： 1348-2246

Isolation and quantification of messenger RNA from tissue models by using a double-barrel carbon probe Peer-reviewed

Yuji Nashimoto, Yasufumi Takahashi, Ryosuke Takano, Kosuke Miyashita, Shukuyo Yamada, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Analytical and Bioanalytical Chemistry　406　(1)　275-282　2014/01

DOI： 10.1007/s00216-013-7430-z 　

Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device Peer-reviewed

Kosuke Ino, Takehito Goto, Yusuke Kanno, Kumi Y. Inoue, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　14　(4)　787-794　2014

DOI： 10.1039/c3lc51156a 　

ISSN： 1473-0197

eISSN： 1473-0189

A screen-printed endotoxin sensor based on amperometry using novel p-aminophenol conjugated substrate for Limulus amebocyte lysate protease reaction Peer-reviewed

Kumi Y. Inoue, Shinichiro Takano, Satoko Takahashi, Yosuke Ishida, Kosuke Ino, Hitoshi Shikuabc, Tomokazu Matsue

Analyst　138　(21)　6523-6531　2013/11/07

DOI： 10.1039/c3an01202f 　

Noninvasive measurement of respiratory activity of mouse embryoid bodies and its correlation with mRNA levels of undifferentiation/differentiation markers Peer-reviewed

Hitoshi Shiku, Toshiharu Arai, Yuanshu Zhou, Nana Aoki, Taku Nishijo, Yoshiko Horiguchi, Kosuke Ino, Tomokazu Matsue

Molecular BioSystems　9　(11)　2701-2711　2013/11

DOI： 10.1039/c3mb70223e 　

Non-invasive measurement of alkaline phosphatase activity in embryoid bodies and co-culture spheroids with scanning electrochemical microscopy Peer-reviewed

Toshiharu Arai, Taku Nishijo, Yoshiharu Matsumae, Yuanshu Zhou, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Analytical Chemistry　85　(20)　9647-9654　2013/10/15

DOI： 10.1021/ac401824q 　

LSI-based amperometric sensor for real-time monitoring of embryoid bodies Peer-reviewed

Mustafa Sen, Kosuke Ino, Kumi Y. Inoue, Toshiharu Arai, Taku Nishijo, Atsushi Suda, Ryota Kunikata, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　48　12-18　2013/10

DOI： 10.1016/j.bios.2013.03.069 　

ISSN： 0956-5663

Electrochemical device with interdigitated ring array electrodes for investigating the relationship between cardiomyocyte differentiation from embryonic stem cells and alkaline phosphatase activity Peer-reviewed

Kosuke Ino, Taku Nishijo, Yusuke Kanno, Fumisato Ozawa, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Electrochemistry　81　(9)　682-687　2013/09

DOI： 10.5796/electrochemistry.81.682 　

ISSN： 1344-3542 2186-2451

Evaluation of the differentiation status of single embryonic stem cells by using scanning electrochemical microscopy Peer-reviewed

Yoshiharu Matsumae, Toshiharu Arai, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Chemical Communications　49　(58)　6498-6500　2013/07/25

DOI： 10.1039/c3cc43126f 　

Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene-expression profiles Peer-reviewed

Yuanshu Zhou, Toshiharu Arai, Yoshiko Horiguchi, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

Analytical Biochemistry　439　(2)　187-193　2013/04/27

DOI： 10.1016/j.ab.2013.04.020 　

Carbon-Ag/AgCl probes for detection of cell activity in droplets Peer-reviewed

Kosuke Ino, Kaoru Ono, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Analytical Chemistry　85　(8)　3832-3835　2013/04/16

DOI： 10.1021/ac303569t 　

ISSN： 0003-2700 1520-6882

Electrodeposition of alginate gels for construction of vascular-like structures Peer-reviewed

Fumisato Ozawa, Kosuke Ino, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Journal of Bioscience and Bioengineering　115　(4)　459-461　2013/04

DOI： 10.1016/j.jbiosc.2012.10.014 　

A contactless electrical stimulator: application to fabricate functional skeletal muscle tissue Peer-reviewed

Samad Ahadian, Javier Ramón-Azcón, Serge Ostrovidov, Gulden Camci-Unal, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

Biomedical Microdevices　15　(1)　109-115　2013/02

DOI： 10.1007/s10544-012-9692-1 　

Alginate gel microwell arrays using electrodeposition for three-dimensional cell culture Peer-reviewed

Fumisato Ozawa, Kosuke Ino, Toshiharu Arai, Javier Ramon-Azcon, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　13　(15)　3128-3135　2013

DOI： 10.1039/c3lc50455g 　

ISSN： 1473-0197

Cell pairing using a dielectrophoresis-based device with interdigitated array electrodes Peer-reviewed

Mustafa Sen, Kosuke Ino, Javier Ramon-Azcon, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　13　(18)　3650-3652　2013

DOI： 10.1039/c3lc50561h 　

ISSN： 1473-0197

eISSN： 1473-0189

Real-time monitoring biomarker expression of carcinoma cells by surface plasmon resonance biosensors Peer-reviewed

Chang Liu, Tingjun Lei, Kosuke Ino, Tomokazu Matsue, Nongjian Tao, Chen-Zhong Li

Chemical Communications　48　(84)　10389-10391　2012/10/28

DOI： 10.1039/c2cc34853e 　

LSI-based amperometric sensor for bio-imaging and multi-point biosensing Peer-reviewed

Kumi Y. Inoue, Masahki Matsudaira, Reyushi Kubo, Masanori Nakano, Shinya Yoshida, Sakae Matsuzaki, Atsushi Suda, Ryota Kunikata, Tatsuo Kimura, Ryota Tsurumi, Toshihito Shioya, Kosuke Ino, Hitoshi Shiku, Shiro Satoh, Masayoshi Esashi, Tomokazu Matsue

Lab on a Chip　12　(18)　3481-3490　2012/09/21

DOI： 10.1039/c2lc40323d 　

Interdigitated array of Pt electrodes for electrical stimulation and engineering of aligned muscle tissue Peer-reviewed

Samad Ahadian, Javier Ramón-Azcón, Serge Ostrovidov, Gulden Camci-Unal, Vahid Hosseini, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

Lab on a Chip　12　(18)　3491-3503　2012/09/21

DOI： 10.1039/c2lc40479f 　

Novel Electrochemical Methodology for Activity Estimation of Alkaline Phosphatase Based on Solubility Difference Peer-reviewed

Kosuke Ino, Yusuke Kanno, Toshiharu Arai, Kumi Y. Inoue, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　84　(18)　7593-7598　2012/09

DOI： 10.1021/ac301429n 　

ISSN： 0003-2700

Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells Peer-reviewed

Javier Ramón-Azcón, Samad Ahadian, Raquel Obregón, Gulden Camci-Unal, Serge Ostrovidov, Vahid Hosseini, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

Lab on a Chip　12　(16)　2959-2969　2012/08/21

DOI： 10.1039/c2lc40213k 　

A new electrochemical assay method for gene expression using hela cells with a secreted alkaline phosphatase (SEAP) reporter system Peer-reviewed

Mustafa Sen, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

BIOTECHNOLOGY AND BIOENGINEERING　109　(8)　2163-2167　2012/08

DOI： 10.1002/bit.24461 　

ISSN： 0006-3592

eISSN： 1097-0290

A Pt layer/Pt disk electrode configuration to evaluate respiration and alkaline phosphatase activities of mouse embryoid bodies Peer-reviewed

Raquel Obregon, Yoshiko Horiguchi, Toshiharu Arai, Shihomi Abe, Yuanshu Zhou, RyosukeTakahashi, Akiko Hisada, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Talanta　94　30-35　2012/05/30

DOI： 10.1016/j.talanta.2012.01.059 　

Development of an electrochemical Limulus amebocyte lysate assay technique for portable and highly sensitive endotoxin sensor Peer-reviewed

Kumi Y Inoue, Satoko Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Innate Immunity　18　(2)　343-349　2012/04/01

DOI： 10.1177/1753425911410337 　

Microfluidic heavy metal immunoassay based on absorbance measurement Peer-reviewed

Date, Y., Terakado, S., Sasaki, K., Aota, A., Matsumoto, N., Shiku, H., Ino, K., Watanabe, Y., Matsue, T., Ohmura, N.

Biosensors and Bioelectronics　1　(15)　106-112　2012/03/15

DOI： 10.1016/j.bios.2011.12.030 　

Local Redox-Cycling-Based Electrochemical Chip Device with Deep Microwells for Evaluation of Embryoid Bodies Peer-reviewed

Kosuke Ino, Taku Nishijo, Toshiharu Arai, Yusuke Kanno, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　51　(27)　6648-6652　2012

DOI： 10.1002/anie.201201602 　

ISSN： 1433-7851

Electrochemical detection for dynamic analyses of a redox component in droplets using a local redox cycling-based electrochemical (LRC-EC) chip device Peer-reviewed

Kosuke Ino, Yusuke Kanno, Taku Nishijo, Takehito Goto, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

CHEMICAL COMMUNICATIONS　48　(68)　8505-8507　2012

DOI： 10.1039/c2cc34264b 　

ISSN： 1359-7345

Accumulation and detection of secreted proteins from single cells for reporter gene assays using a local redox cycling-based electrochemical (LRC-EC) chip device Peer-reviewed

Mustafa Sen, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　12　(21)　4328-4335　2012

DOI： 10.1039/c2lc40674h 　

ISSN： 1473-0197

Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device Peer-reviewed

Xi Zhu, Kosuke Ino, Zhenyu Lin, Hitoshi Shiku, Guonan Chena, Tomokazu Matsue

Sensors & Actuators: B. Chemical　160　(1)　923-928　2011/12/15

DOI： 10.1016/j.snb.2011.09.004 　

Monitoring oxygen consumption of single mouse embryos using an integrated electrochemical microdevice Peer-reviewed

Yasumoto Date, Shinichiro Takano, Hitoshi Shiku, Kosuke Ino, Takahiro Ito-Sakaki, Masaki Yokoo, Hiroyuki Abe, Tomokazu Matsue

Biosensors and Bioelectronics　30　(1)　100-106　2011/12/15

DOI： 10.1016/j.bios.2011.08.037 　

Electrochemical chip integrating scalable ring-ring electrode array to detect secreted alkaline phosphatase Peer-reviewed

Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu Matsue

Analyst　136　(23)　4991-4996　2011/10/06

DOI： 10.1039/c1an15620a 　

Electrochemical detection of receptor-mediated endocytosis by scanning electrochemical microscopy Peer-reviewed

Yasufumi Takahashi, Takeshi Miyamoto, Hitoshi Shiku, Kosuke Ino, Tomoyuki Yasukawa, Ryutaro Asano, Izumi Kumagai, Tomokazu Matsue

Physical Chemistry Chemical Physics　13　(37)　16569-16573　2011/08/17

DOI： 10.1039/c1cp21886g 　

Electrorotation chip consisting of three-dimensional interdigitated array electrodes Peer-reviewed

Kosuke Ino, Atsuko Ishida, Kumi Y. Inoue, Masato Suzuki, Masahiro Koide, Tomoyuki Yasukawa, Hitoshi Shikua, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　153　(2)　468-473　2011/04

DOI： 10.1016/j.snb.2010.11.012 　

ISSN： 0925-4005

Influence of tip size on single yeast cell imaging using scanning electrochemical microscopy Peer-reviewed

Kuniaki Nagamine, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Electroanalysis　23　(5)　1168-1174　2011/03/18

DOI： 10.1002/elan.201000595 　

Addressable electrode array device with IDA electrodes for high-throughput detection Peer-reviewed

Kosuke Ino, Wataru Saito, Masahiro Koide, Taizo Umemura, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　11　(3)　385-388　2011

DOI： 10.1039/c0lc00437e 　

ISSN： 1473-0197

Chronoamperometric characterization of secreted alkaline phosphatase from single-cell entrapped in a poly(dimethylsiloxisane) microwell Peer-reviewed

Hitoshi Shiku, Junya Suzuki, Tatsuya Murata, Kosuke Ino, Tomokazu Matsue

Electrochimica Acta　27　(30)　8263-8267　2010/11

DOI： 10.1016/j.electacta.2010.04.011 　

Reporter gene expression at single-cell level characterized with real-time RT-PCR, chemiluminescence, fluorescence, and electrochemical imaging Peer-reviewed

Hitoshi Shiku, Daisuke Okazaki, Junya Suzuki, Yasufumi Takahashi, Tatsuya Murata, Hidetaka Akita, Hideyoshi Harashima, Kosuke Ino, Tomokazu Matsue

FEBS letters　584　(18)　4000-4008　2010/09

DOI： 10.1016/j.febslet.2010.08.008 　

Electrochemical detection of endotoxin using recombinant factor C zymogen Peer-reviewed

Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Electrochemistry Communications　12　(8)　1066-1069　2010/08

DOI： 10.1016/j.elecom.2010.05.028 　

Rapid and simple immunosensing system for simultaneous detection of tumor markers based on negative-dielectrophoretic manipulation of microparticles Peer-reviewed

Hyun Jung Lee, Sang Hyun Lee, Tomoyuki Yasukawa, Javier Ramón-Azcón, Fumio Mizutani, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Talanta　81　(1-2)　657-663　2010/04/15

DOI： 10.1016/j.talanta.2009.12.058 　

Electrochemical monitoring of hydrogen peroxide released from leucocytes on horseradish peroxidase redox polymer coated electrode chip Peer-reviewed

Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku, Shigenobu Kasai, Tomoyuki Yasukawa, Fumio Mizutani, Tomokazu Matsue

Biosensors and Bioelectronics　25　(7)　1723-1728　2010/03/15

DOI： 10.1016/j.bios.2009.12.014 　

Magnetic manipulation device for the optimization of cell processing conditions Invited Peer-reviewed

Hiroshi Ito, Ryuji Kato, Kosuke Ino, Hiroyuki Honda

Journal of Bioscience and Bioengineering　109　(2)　182-188　2010/02

DOI： 10.1016/j.jbiosc.2009.07.006 　

Electrochemical topography of a cell monolayer with an addressable microelectrode array Peer-reviewed

Zhenyu Lin, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Chemical Communications　46　(4)　559-561　2010/01

DOI： 10.1039/b915212a 　

Addressable electrochemiluminescence detection system based on redox-cycling of Ru(bpy)(3)(2+) Peer-reviewed

Zhenyu Lin, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue, Guonan Chen

Chemical Communications　46　(2)　243-245　2010/01

DOI： 10.1039/b915871e 　

Manipulation of Microparticles for Construction of Array Patterns by Negative Dielectrophoresis Using Multilayered Array and Grid Electrodes Peer-reviewed

Kosuke Ino, Hitoshi Shiku, Fumisato Ozawa, Tomoyuki Yasukawa, Tomokazu Matsue

BIOTECHNOLOGY AND BIOENGINEERING　104　(4)　709-718　2009/11

DOI： 10.1002/bit.22445 　

ISSN： 0006-3592

Microfluid-assisted dielectrophoretic alignment and device characterization of single ZnO wires Invited Peer-reviewed

Sang Hyun Lee, Hyun Jung Lee, Kosuke Ino, Hitoshi Shiku, Takafumi Yao, Tomokazu Matsue

Journal of Physical Chemistry C　113　(45)　19376-19381　2009/10/20

DOI： 10.1021/jp908161v 　

Detection of hormone active chemicals using genetically engineered yeast cells and microfluidic devices with interdigitated array electrodes Peer-reviewed

Kosuke Ino, Yusuke Kitagawa, Tsuyoshi Watanabe, Hitoshi Shiku, Masahiro Koide, Tomoaki Itayama, Tomoyuki Yasukawa, Tomokazu Matsue

ELECTROPHORESIS　30　(19)　3406-3412　2009/10

DOI： 10.1002/elps.200900244 　

ISSN： 0173-0835

Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays Peer-reviewed

Zhenyu Lin, Yasufumi Takahashi, Tatsuya Murata, Michiaki Takeda, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Angewandte Chemie International Edition　48　(11)　2009/02

DOI： 10.1002/anie.200805743 　

Application of Magnetic Force-Based Cell Patterning for Controlling Cell-Cell Interactions in Angiogenesis Peer-reviewed

Kosuke Ino, Mina Okochi, Hiroyuki Honda

BIOTECHNOLOGY AND BIOENGINEERING　102　(3)　882-890　2009/02

DOI： 10.1002/bit.22104 　

ISSN： 0006-3592

Plasmid DNA Transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet Peer-reviewed

Kosuke Ino, Tamayo Kawasumi, Akira Ito, Hiroyuki Honda

BIOTECHNOLOGY AND BIOENGINEERING　100　(1)　168-176　2008/05

DOI： 10.1002/bit.21738 　

ISSN： 0006-3592

Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis Peer-reviewed

Kosuke Ino, Mina Okochi, Nao Konishi, Masahiro Nakatochi, Rentaro Imai, Mitsuhiro Shikida, Akira Ito, Hiroyuki Honda

LAB ON A CHIP　8　(1)　134-142　2008

DOI： 10.1039/b712330b 　

ISSN： 1473-0197

Application of ultra-water-repellent surface to cell culture Peer-reviewed

Kosuke Ino, Akira Ito, Yunying Wu, Nagahiro Saito, Eri Hibino, Osamu Takai, Hiroyuki Honda

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　104　(5)　420-423　2007/11

DOI： 10.1263/jbb.104.420 　

ISSN： 1389-1723

Cell patterning using magnetite nanoparticles and magnetic force Peer-reviewed

Kosuke Ino, Akira Ito, Hiroyuki Honda

BIOTECHNOLOGY AND BIOENGINEERING　97　(5)　1309-1317　2007/08

DOI： 10.1002/bit.21322 　

ISSN： 0006-3592

Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering Peer-reviewed

Kosuke Ino, Akira Ito, Hirohito Kumazawa, Hideaki Kagami, Minoru Ueda, Hiroyuki Honda

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN　40　(1)　51-58　2007/01

DOI： 10.1252/jcej.40.51 　

ISSN： 0021-9592

The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting Peer-reviewed

Akira Ito, Kousuke Ino, Takeshi Kobayashi, Hiroyuki Honda

Biomaterials　26　(31)　6185-6193　2005/12

DOI： 10.1016/j.biomaterials.2005.03.039 　

Novel methodology for fabrication of tissue-engineered tubular constructs using magnetite nanoparticles and magnetic force Peer-reviewed

Akira Ito, Kousuke Ino, Masao Hayashida, Takeshi Kobayashi, Hiroshi Matsunuma, Hideki Kagami, Minoru Ueda, Hiroyuki Honda

Tissue Engineering　11　(9-10)　1553-1561　2005/09

DOI： 10.1089/ten.2005.11.1553