Details of the Researcher

PHOTO

Shun Itai
Section
Graduate School of Biomedical Engineering
Job title
Assistant Professor
Degree
  • 博士(工学)(慶應義塾大学)

  • 修士(工学)(慶應義塾大学)

e-Rad No.
90969007

Research History 7

  • 2025/04 - Present
    Keio University Graduate School of Science and Technology

  • 2025/04 - Present
    Tohoku University Graduate School of Biomedical Engineering Assistant Professor

  • 2024/07 - Present
    慶應義塾湘南藤沢中高等部 臨時職員

  • 2022/04 - 2025/03
    Keio University Graduate School of Science and Technology

  • 2022/04 - 2025/03
    Japan Society for the Promotion of Science Research Fellowship for Young Scientists PD

  • 2020/04 - 2022/03
    Japan Society for the Promotion of Science Research Fellowship for Young Scientists DC2

  • 2019/04 - 2020/03
    Keio University Graduate School of Science and Technology Assistant Professor (Defined term, Research Encouraged)

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Education 3

  • Keio University Graduate School of Science and Technology School of Integrated Design Engineering

    2019/04 - 2022/03

  • Keio University Graduate School of Science and Technology School of Integrated Design Engineering

    2017/04 - 2019/03

  • Keio University Faculty of Science and Technology Department of Mechanical Engineering

    2013/04 - 2017/03

Committee Memberships 3

  • 日本生体医工学会 若手WG幹事

    2024/05 - Present

  • 日本生体医工学会 理事会幹事

    2023/09 - Present

  • Japanese Society for Medical and Biological Engineering Working group of young researcheres

    2022/06 - Present

Professional Memberships 4

  • Japanese Society for Medical and Biological Engineering

    2021/05 - Present

  • The Japanese Society for Regenerative Medicine

    2020/02 - Present

  • 化学とマイクロ・ナノシステム学会

    2018/08 - Present

  • The Japan Society of Mechanical Engineers

    2017/05 - Present

Research Interests 5

  • 機械学習

  • 血流解析

  • Tissue Engineered Blood Vessel

  • Biomedical Engineering

  • Tissue Engineering

Research Areas 3

  • Nanotechnology/Materials / Material fabrication and microstructure control /

  • Life sciences / Biomedical engineering /

  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Machine materials and mechanics /

Awards 11

  1. Inoue Research Award for Young Scientists

    2025/02 Inoue Foundation for Science

  2. 優秀研究賞

    2024/11 化学とマイクロ・ナノシステム学会 螺旋状の高密度平滑筋層を含有し生理的収縮弛緩機能を実現したiPS由来人工血管による老化病態再現

  3. Catalyst Award

    2024/09 U.S. National Academy of Medicine Aiming for Vascular Regeneration and Rejuvenation Using iPSC-3D Vasculature

  4. CBMS Student/Young Researcher Travel Grant

    2024/09 Chemical and Biological Microsystems Society AN iPSC-BLOOD VESSEL WITH SPIRALLY ORIENTED SMOOTH MUSCLE MIMIC AGING PHENOTYPES IN PHYSIOLOGICAL FUNCTION AND METABOLITES

  5. ベストポスターアワード

    2024/09 日本生体医工学会 螺旋状に配向した高密度平滑筋層を持つiPS由来人工血管を用いた血管収縮・弛緩機能における病態再現

  6. Fujiwara Award

    2022/03 Keio University

  7. Grand Prize of Young Investigator’s Award

    2021/06 Japanese Society for Medical and Biological Engineering

  8. Outstanding Presentation Award

    2018/11 Society for Chemistry and Micro-Nano Systems

  9. Outstanding Presentation Award of Young Fellow

    2018/06 The Japan Society of Mechanical Engineers

  10. Outstanding poster award

    2017/11 The Institute of Electrical Engineers of Japan

  11. Kito Award

    2017/03 Keio University

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Papers 17

  1. Maturation of Human iPSC‐Derived Cardiac Microfiber with Electrical Stimulation Device Peer-reviewed

    Akari Masuda, Yuta Kurashina, Hidenori Tani, Yusuke Soma, Jumpei Muramatsu, Shun Itai, Shugo Tohyama, Hiroaki Onoe

    Advanced Healthcare Materials 2024/05/29

    Publisher: Wiley

    DOI: 10.1002/adhm.202303477  

    ISSN: 2192-2640

    eISSN: 2192-2659

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    Abstract Here an electrical stimulation system is described for maturing microfiber‐shaped cardiac tissue (cardiac microfibers, CMFs). The system enables stable culturing of CMFs with electrical stimulation by placing the tissue between electrodes. The electrical stimulation device provides an electric field covering whole CMFs within the stimulation area and can control the beating of the cardiac microfibers. In addition, CMFs under electrical stimulation with different frequencies are examined to evaluate the maturation levels by their sarcomere lengths, electrophysiological characteristics, and gene expression. Sarcomere elongation (14% increase compared to control) is observed at day 10, and a significant upregulation of electrodynamic properties such as gap junction protein alpha 1 (GJA1) and potassium inwardly rectifying channel subfamily J member 2 (KCNJ2) (maximum fourfold increase compared to control) is observed at day 30. These results suggest that electrically stimulated cultures can accelerate the maturation of microfiber‐shaped cardiac tissues compared to those without electrical stimulation. This model will contribute to the pathological research of unexplained cardiac diseases and pharmacologic testing by stably constructing matured CMFs.

  2. Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force Measurement Peer-reviewed

    Yuta Kurashina, Keisuke Fukada, Shun Itai, Shuichi Akizuki, Ryo Sato, Akari Masuda, Hidenori Tani, Jun Fujita, Keiichi Fukuda, Shugo Tohyama, Hiroaki Onoe

    Advanced Science 10 (35) 2023/10/17

    Publisher: Wiley

    DOI: 10.1002/advs.202301831  

    ISSN: 2198-3844

    eISSN: 2198-3844

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    Abstract In vitro reconstruction of highly mature engineered heart tissues (EHTs) is attempted for the selection of cardiotoxic drugs suitable for individual patients before administration. Mechanical contractile force generated in the EHTs is known to be a critical indicator for evaluating the EHT response. However, measuring contractile force requires anchoring the EHT in a tailored force‐sensing cell culture chamber, causing technical difficulties in the stable evaluation of contractile force in long‐term culture. This paper proposes a hydrogel‐sheathed human induced pluripotent stem cell (hiPSC)‐derived heart microtissue (H3M) that can provide an anchor‐free contractile force measurement platform in commonly used multi‐well plates. The contractile force associated with tissue formation and drug response is calculated by motion tracking and finite element analysis on the bending angle of the hydrogel sheath. From the experiment of the drug response, H3M is an excellent drug screening platform with high sensitivity and early testing capability compared to conventionally anchored EHT. This unique platform would be useful and versatile for regenerative therapy and drug discovery research in EHT.

  3. Schwann cell-encapsulated chitosan-collagen hydrogel nerve conduit promotes peripheral nerve regeneration in rodent sciatic nerve defect models Peer-reviewed

    Hiroaki Takeya, Shun Itai, Hiroo Kimura, Yuta Kurashina, Tsuyoshi Amemiya, Narihito Nagoshi, Takuji Iwamoto, Kazuki Sato, Shinsuke Shibata, Morio Matsumoto, Hiroaki Onoe, Masaya Nakamura

    Scientific Reports 13 (1) 2023/07/24

    Publisher: Springer Science and Business Media LLC

    DOI: 10.1038/s41598-023-39141-2  

    eISSN: 2045-2322

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    Abstract Chitosan has various tissue regeneration effects. This study was designed to investigate the nerve regeneration effect of Schwann cell (SC)-encapsulated chitosan-collagen hydrogel nerve conduit (CCN) transplanted into a rat model of sciatic nerve defect. We prepared a CCN consisting of an outer layer of chitosan hydrogel and an inner layer of collagen hydrogel to encapsulate the intended cells. Rats with a 10-mm sciatic nerve defect were treated with SCs encapsulated in CCN (CCN+), CCN without SCs (CCN−), SC-encapsulated silicone tube (silicone+), and autologous nerve transplanting (auto). Behavioral and histological analyses indicated that motor functional recovery, axonal regrowth, and myelination of the CCN+ group were superior to those of the CCN− and silicone+ groups. Meanwhile, the CCN− and silicone+ groups showed no significant differences in the recovery of motor function and nerve histological restoration. In conclusion, SC-encapsulated CCN has a synergistic effect on peripheral nerve regeneration, especially axonal regrowth and remyelination of host SCs. In the early phase after transplantation, SC-encapsulated CCNs have a positive effect on recovery. Therefore, using SC-encapsulated CCNs may be a promising approach for massive peripheral nerve defects.

  4. Unusual Selective Monitoring of N,N-Dimethylformamide in a Two-Dimensional Material Field-Effect Transistor Peer-reviewed

    Akito Fukui, Keigo Matsuyama, Hiroaki Onoe, Shun Itai, Hidekazu Ikeno, Shunsuke Hiraoka, Kousei Hiura, Yuh Hijikata, Jenny Pirillo, Takahiro Nagata, Kuniharu Takei, Takeshi Yoshimura, Norifumi Fujimura, Daisuke Kiriya

    ACS Nano 2023/07/17

    Publisher: American Chemical Society (ACS)

    DOI: 10.1021/acsnano.3c03915  

    ISSN: 1936-0851

    eISSN: 1936-086X

  5. Flexibly Deformable Collagen Hydrogel Tube Reproducing Immunological Tissue Deformation of Blood Vessels as a Pharmacokinetic Testing Model Peer-reviewed

    Shun Itai, Hiroaki Onoe

    Advanced Healthcare Materials 11 (1) 2101509-2101509 2022/10/25

    Publisher: Wiley

    DOI: 10.1002/adhm.202101509  

    ISSN: 2192-2640

    eISSN: 2192-2659

  6. Janus Hydrogel Microbeads for Glucose Sensing with pH Calibration Peer-reviewed

    Maru Ando, Mio Tsuchiya, Shun Itai, Tomomi Murayama, Yuta Kurashina, Yun Jung Heo, Hiroaki Onoe

    Sensors 21 (14) 4829-4829 2021/07/15

    Publisher: MDPI AG

    DOI: 10.3390/s21144829  

    eISSN: 1424-8220

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    We present fluorescent Janus hydrogel microbeads for continuous glucose sensing with pH calibration. The Janus hydrogel microbeads, that consist of fluorescent glucose and pH sensors, were fabricated with a UV-assisted centrifugal microfluidic device. The microbead can calibrate the pH values of its surroundings and enables accurate measurements of glucose within various pH conditions. As a proof of concept, we succeeded in obtaining the accurate value of glucose concentration in a body-fluid-like sample solution. We believe that our fluorescent microbeads, with pH calibration capability, could be applied to fully implantable sensors for continuous glucose monitoring.

  7. Endothelial Chemical Reaction and Drug Test Reproduced on Molded Flexible Collagen Hydrogel Tube

    Shun Itai, Hiroaki Onoe

    21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021 283-286 2021/06/20

    Publisher: Institute of Electrical and Electronics Engineers Inc.

    DOI: 10.1109/Transducers50396.2021.9495658  

  8. Travelling ultrasound promotes vasculogenesis of three‐dimensional‐monocultured human umbilical vein endothelial cells Peer-reviewed

    Chikahiro Imashiro, Tetsuya Azuma, Shun Itai, Taiki Kuribara, Kiichiro Totani, Hiroaki Onoe, Kenjiro Takemura

    Biotechnology and Bioengineering 118 (10) 3760-3769 2021/06/10

    Publisher: Wiley

    DOI: 10.1002/bit.27852  

    ISSN: 0006-3592

    eISSN: 1097-0290

  9. Cell-encapsulated chitosan-collagen hydrogel hybrid nerve guidance conduit for peripheral nerve regeneration Peer-reviewed

    Shun Itai, Karin Suzuki, Yuta Kurashina, Hiroo Kimura, Tsuyoshi Amemiya, Kazuki Sato, Masaya Nakamura, Hiroaki Onoe

    Biomedical Microdevices 22 (4) 2020/12

    Publisher: Springer Science and Business Media LLC

    DOI: 10.1007/s10544-020-00536-x  

    ISSN: 1387-2176

    eISSN: 1572-8781

  10. ECM-based microfluidic gradient generator for tunable surface environment by interstitial flow Peer-reviewed

    Azusa Shimizu, Wei Huang Goh, Shun Itai, Rahul Karyappa, Michinao Hashimoto, Hiroaki Onoe

    Biomicrofluidics 14 (4) 044106-044106 2020/07/16

    Publisher: AIP Publishing

    DOI: 10.1063/5.0010941  

    eISSN: 1932-1058

  11. ECM-based microchannel for culturing in vitro vascular tissues with simultaneous perfusion and stretch Peer-reviewed

    Azusa Shimizu, Wei Huang Goh, Shun Itai, Michinao Hashimoto, Shigenori Miura, Hiroaki Onoe

    Lab on a Chip 20 (11) 1917-1927 2020/04/20

    Publisher: Royal Society of Chemistry (RSC)

    DOI: 10.1039/d0lc00254b  

    ISSN: 1473-0197

    eISSN: 1473-0189

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    <p>A perfusable and stretchable gelatin-based microfluidic system that can apply both simultaneous fluidic shear stress and stretch stress to in vitro endothelial 3D tissues is presented.</p>

  12. Wireless and battery-free digestible sensor for intestinal bacteria monitoring

    Ayaka Inami, Erika Iyama, Shun Itai, Hiroaki Onoe

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 575-576 2020

    Publisher: Chemical and Biological Microsystems Society

  13. Double-layer collagen microtube for perfusable heterogeneous culture

    Shun Itai, Hiroaki Onoe

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 1137-1138 2020

    Publisher: Chemical and Biological Microsystems Society

  14. Stretching motion-driven ECM-based pulsatile flow generator for mimicking venous blood flow in vivo

    Azusa Shimizu, Wei Huang Goh, Shun Itai, Michinao Hashimoto, Sigenori Miura, Hiroaki Onoe

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 314-315 2019

    Publisher: Chemical and Biological Microsystems Society

  15. Cell-encapsulating chitosan-collagen hybrid hydrogel conduit for peripheral nerve regeneration

    Shun Itai, Karin Suzuki, Yuta Kurashina, Hiroo Kimura, Tsuyoshi Amemiya, Kazuki Sato, Masaya Nakamura, Hiroaki Onoe

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 880-881 2019

    Publisher: Chemical and Biological Microsystems Society

  16. Thin Layered Heterogeneous Vascularized 3D Tissue Models Constructed with Separated-Layer Collagen Microtube

    Shun Itai, Hiroaki Onoe

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) 2019- 118-119 2019/01/01

    Publisher: Institute of Electrical and Electronics Engineers Inc.

    DOI: 10.1109/MEMSYS.2019.8870866  

    ISSN: 1084-6999

  17. Double-layer perfusable collagen microtube device for heterogeneous cell culture Peer-reviewed

    Shun Itai, Hisatsugu Tajima, Hiroaki Onoe

    Biofabrication 11 (1) 015010-015010 2018/11/30

    Publisher: IOP Publishing

    DOI: 10.1088/1758-5090/aaf09b  

    eISSN: 1758-5090

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Books and Other Publications 1

  1. ケミカルエンジニアリング バイオテクノロジーが拓く新時代

    板井駿, 尾上弘晃

    化学工業社 2018/11

Presentations 22

  1. In vitro Tube-shaped Intestinal Model with a Crypt-like Inner Surface Created by Electrolytic Microbubbles

    Shuma Tanaka, Shun Itai, Hiroaki Onoe

    The 10th International Conference on BioSensors, BioElectronics, BioMedical Devices, BioMEMS/NEMS & Applications (Bio4Apps 2024)

  2. An iPSC-blood vessel with spirally oriented smooth muscle mimic aging phenotypes in physiological function and metabolites

    S. Itai, T. Toyohara, H. Onoe, T. Abe

    The 28th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2024) 2024/10/15

  3. 3D perfusable in vitro intestinal tube-shaped device with cryptic structure covered by mucin layer for bacterial co-culture

    S. Uramoto, S. Tanaka, S. Itai, H. Onoe

    The 28th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2024) 2024/10/15

  4. Mucin-layer-secreting in vitro intestinal tube-shaped device with crypt structure for bacterial co-culture

    Shota Uramoto, Shuma Tanaka, Shun Itai, Hiroaki Onoe

    The 37th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2024)

  5. Real-Time Assessment of Maturity by Microfiber Shaped Ipscs-Derived Cardiac Tissue

    Akari Masuda, Shun Itai, Yuta Kurashina, Shugo Tohyama, Hiroaki Onoe

    The 22nd International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2023)

  6. An engineered human iPSC-blood vessel with organized spiral oriented smooth muscle layers as a new functional model for cardiovascular diseases

    Shun Itai, Mayuko Korekata, Takafumi Toyohara, Hiroaki Onoe, Takaaki Abe

    International society for stem cell research (ISSCR) annual meeting 2023

  7. Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells

    Chisaki Yamagata, Shun Itai, Yuta Kurashina, Makoto Asai, Ayuko Hoshino, Hiroaki Onoe

    The 36th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2023)

  8. Controlling Firing Point of Microfiber-Shaped Hipsc-Derived Cardiac Tissue with Localized Electrical Stimulation Device

    Akari Masuda, Shun Itai, Yuta Kurashina, Shugo Tohyama, Hiroaki Onoe

    The 36th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2023)

  9. A THREE-DIMENSIONAL ARTIFICIAL INTESTINAL TUBE WITH A CRYPT-LIKE INNER SURFACE

    Shuma Tanaka, Shun Itai, Hiroaki Onoe

    The 36th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2023)

  10. UNIDIRECTIONAL ANALYSIS OF CARDIAC PROPAGATION VELOCITY BY HUMAN IPSC-DERIVED CARDIAC CORE-SHELL MICROFIBER

    Akari Masuda, Shun Itai, Yuta Kurashina, Shugo Tohyama, Hiroaki Onoe

    The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2022)

  11. OIL-SEALED HYDROGEL MICROWELL ARRAY FOR ANALYSIS ON SECRETORY COMPONENTS FROM CONFINED SINGLE CELLS

    Chisaki Yamagata, Shun Itai, Yuta Kurashina, Makoto Asai, Ayuko Hoshino, Hiroaki Onoe

    The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2022)

  12. A THREE-DIMENSIONAL ARTIFICIAL INTESTINAL TUBE WITH A CRYPT-LIKE INNER SURFACE FORMED BY ELECTROLYTIC MICROBUBBLES

    Shuma Tanaka, Shun Itai, Hiroaki Onoe

    The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2022) 2022/10/25

  13. Fixation-free evaluation of cardiac contractile force by human iPSC-derived cardiac core-shell microfiber

    Akari Masuda, Keisuke Fukada, Shun Itai, Yuta Kurashina, Shuichi Akizuki, Shugo Tohyama, Jun Fujita, Hiroaki Onoe

    The 35th International Conference on Micro Electro Mechanical Systems (MEMS 2022) 2022/01/12

  14. Tube-shaped in-vitro intestinal gut model with 3D isotropic medium supply for bacterial symbiosis

    Mitsuki Kawahara, Shun Itai, Hiroaki Onoe

    The 35th International Conference on Micro Electro Mechanical Systems (MEMS 2022) 2022/01/12

  15. In vitro artery model with circumferentially aligned & contractible smooth muscle by unfixed molding & screwing fabrication

    Shun Itai, Hiroaki Onoe

    The 35th International Conference on Micro Electro Mechanical Systems (MEMS 2022) 2022/01/12

  16. GLUCOSE-MONITORING JANUS HYDROGEL MICROBEADS WITH COMPENSATION FUNCIONS FOR SURROUNDING PH ENVIRONMENT

    Maru Ando, Mio Tsuchiya, Shun Itai, Yun Jung Heo, Hiroaki Onoe

    The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2021)

  17. Endothelial chemical reaction and drug test reproduced on molded flexible collagen hydrogel tube

    Shun Itai, Hiroaki Onoe

    The 21st International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2021) 2021/06/23

  18. Wireless and battery-free digestible sensor for intestinal bacteria monitoring

    Ayaka Inami, Erika Iyama, Shun Itai, Hiroaki Onoe

    The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicorTAS 2020) 2020/10/05

  19. Stretching motion-driven ECM-based pulsatile flow generator for mimicking venous blood flow in vivo

    Azusa Shimizu, Wei Huang Goh, Shun Itai, Michinao Hashimoto, Shigenori Miura, Hiroaki Onoe

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicorTAS 2019) 2019/10/29

  20. Cell-encapsulating chitosan-collagen hybrid hydrogel conduit for peripheral nerve regeneration

    Shun Itai, Karin Suzuki, Yuta Kurashina, Hiroo Kimura, Tsuyoshi Amemiya, Kazuki Sato, Masaya Nakamura, Hiroaki Onoe

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicorTAS 2019) 2019/10/29

  21. Thin layered heterogeneous vascularized 3D tissue models constructed with separated-layer collagen microtube

    Shun Itai, Hiroaki Onoe

    The 32nd International Conference on Micro Electro Mechanical Systems (MEMS 2019) 2019/01/29

  22. Double-layer collagen microtube for perfusable heterogeneous culture

    Shun Itai, Hiroaki Onoe

    The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicorTAS 2017) 2017/10/23

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Industrial Property Rights 7

  1. 多層構造体とその製造方法及び利用方法

    板井駿, 尾上弘晃

    特許7165330

    Property Type: Patent

  2. 管腔構造体,及び管腔構造体の製造方法

    尾上弘晃, 田中秀磨, 板井駿, 豊原敬文

    Property Type: Patent

  3. 神経再生を促進するための管状構造体

    尾上弘晃, 鈴木果林, 板井駿, 倉科佑太, 木村洋朗, 雨宮剛, 佐藤和毅, 中村雅也

    Property Type: Patent

  4. 分岐人工血管モデルの製造方法

    板井駿, 是方真悠子, 豊原敬文, 尾上弘晃

    Property Type: Patent

  5. 幹細胞を用いた人工組織チューブ及びその製造方法

    板井駿, 豊原敬文

    Property Type: Patent

  6. 人工組織チューブ及びその製造方法

    尾上弘晃, 板井駿

    Property Type: Patent

  7. マイクロウェルアレイ,その製造方法,及び単一細胞由来エクソソームの解析方法

    尾上弘晃, 山形智咲, 板井駿, 倉科佑太, 星野歩子

    Property Type: Patent

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Research Projects 9

  1. 動脈硬化性腎動脈狭窄患者CT画像を基にした生理的に収縮・弛緩可能なiPS由来人工分枝血管の開発による動脈硬化再現と病態解明

    板井駿

    Offer Organization: 武田科学振興財団

    System: 医学系研究助成(基礎)

    2025/07 - 2030/03

  2. 「生きた」人工血管の社会実装

    豊原敬文, 板井駿

    Offer Organization: みちのくアカデミア発スタートアップ共創プラットフォーム(MASP)

    System: みちのくGAPファンド(スタートアップ・エコシステム共創プログラム)

    2025/04 - 2028/03

  3. 自在に収縮・弛緩可能なiPSC人工老化血管モデルの構築による老化治療法研究

    板井 駿

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 若手研究

    Institution: 東北大学

    2023/04 - 2026/03

  4. iPS 細胞由来 3D 血管を用いた血管再生治療の開発と社会実装

    豊原敬文, 板井駿

    Offer Organization: 東北大学

    System: ビジネス・インキュベ-ション・プログラム(BIP)

    2024/04 - 2025/03

  5. 細胞加工ゲルチューブを用いた腸・血管・リンパ共培養による腸内細菌共生人工腸の構築

    板井 駿

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東北大学

    2022/04 - 2025/03

  6. 血管老化治療法開発に向けた自在変形人工血管モデルの開発

    板井駿

    Offer Organization: 公益財団法人 テルモ生命科学振興財団

    System: 研究開発助成金

    Category: ⑤予防医療研究

    Institution: 東北大学

    2023/01 - 2024/03

  7. 多段階ゲル型内組織成熟モールディング法(GG-3M 法)による大血管組織モデル構築

    板井駿

    Offer Organization: 慶應義塾大学

    System: 潮田記念基金による慶應義塾博士課程学生研究支援プログラム

    Institution: 慶應義塾大学

    2021/04 - 2022/03

  8. アトピー性皮膚炎の炎症再現が可能なin vitro皮膚組織モデルの構築

    板井 駿

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 慶應義塾大学

    2020/04 - 2022/03

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    本研究の目的は,灌流共培養可能なコラーゲンチューブ用いて,組織化させた血管の周囲に免疫細胞及び表皮細胞を培養することで血管内包皮膚炎組織モデルを構築し,アレルギー反応に代表される炎症反応を生体外で再現することである. 炎症反応の高いレベルでの再現を達成するためには,血管組織において,細胞レベルのみならず組織レベルでのマクロな炎症応答の模倣が求められる.令和2年度はこれを達成するため,私がこれまでに開発してきたコラーゲンチューブデバイスを改良することで,細胞・組織レベル双方での血管の免疫化学応答の再現を達成した.さらに薬剤流入による薬効試験の模倣にも成功したことで,目指している炎症病理モデルとしての有用性を示した. 具体的には,コラーゲンとシリコーンチューブ,さらにガラス管が直接接合されたデバイスを構築することで,炎症反応や薬剤流入時のデバイスの安定性を飛躍的に向上させた.また,数日の培養により安定的に血管組織の構築(ヒト臍帯静脈内皮細胞(HUVEC)を使用)がなされ,デバイスの寸法精度も変動係数3.3%と非常に高いことが確認された. 炎症反応の再現は,アトピー性皮膚炎の炎症メディエータであるヒスタミンを用いて行った.ヒスタミンに曝露された血管組織は,細胞に発生した力学的な収縮力により組織レベルでマクロな収縮を起こし,生体内と近い挙動を示した.また,組織・細胞双方のスケールでの収縮が起きたことにより,チューブ内壁に占める細胞面積はほとんど変化しておらず,従来報告されている固定された培養足場と比較しても有意差が認められた(p < 0.01)).最後に,抗ヒスタミン薬の成分でもあるオロパタジンを血管モデル内部に流入させ,薬剤反応試験を行った.その結果オロパタジンの流入によって,血管収縮が抑制され,ヒスタミンによる炎症反応を阻害できることが確認された.

  9. 灌流共培養可能なコラーゲンチューブを用いたin vitro 皮膚炎組織モデル構築

    板井駿

    Offer Organization: 慶應義塾大学

    System: 潮田記念基金による慶應義塾博士課程学生研究支援プログラム

    Institution: 慶應義塾大学

    2019/04 - 2020/03

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