Details of the Researcher

PHOTO

Katsuya Uchida
Section
Graduate School of Information Sciences
Job title
Assistant Professor
Degree

  • Ph.D. (Saitama University)

Committee Memberships 12

  • Frontiers in Endocrinology　Editorial Board of Neuroendocrine Science (Review Editor)

    2022/09 - Present

  • Frontiers in Neuroscience　Editorial Board of Neurodevelopment (Review Editor)

    2022/04 - Present

  • 日本神経内分泌学会　評議員

    2012/09 - Present

  • 19th International Congress of Comparative Endocrinology　実行委員

    2018/11 - 2025/11

  • 日本比較内分泌学会　第44回比較内分泌学会 埼玉大会実行委員

    2018/08 - 2019/10

  • 日本内分泌学会　第36回内分泌代謝学サマーセミナー事務局長

    2017/09 - 2018/09

  • 日本神経内分泌学会•第４２回学術集会　実行委員

    2013/10 - 2015/09

  • 日本動物学会・第８５回仙台大会　実行委員

    2013/04 - 2014/09

  • 日本動物学会　支部会計監事

    2011/01 - 2012/09

  • 日本神経科学学会　プログラム委員

    2009/12 - 2011/09

  • 日本動物学会　支部大会実行委員

    2009/01 - 2009/08

  • 日本動物学会　支部会計監事

    2004/04 - 2008/03

Show all ︎Show first 5

Professional Memberships 6

  • JAPAN SOCIETY FOR COMPARATIVE ENDOCRINOLOGY

  • ニューロン新生懇談会

  • 日本甲状腺学会

  • 日本神経内分泌学会

  • Japan Neuroscience Society

  • The Zoological Society of Japan

︎Show all ︎Show first 5

Research Interests 6

  • adult neurogenesis

  • the bed nucleus of the stria terminalis

  • CRH

  • thyroid hormone

  • sex diffrences

  • neurodevelopmental disorder

Research Areas 3

  • Life sciences / Morphology, anatomy /

  • Life sciences / Neuroscience - general /

  • Life sciences / Metabolism and endocrinology /

Awards 1

  1. 成茂神経科学研究助成基金

    2002/09　日本神経科学学会　歯状回における神経細胞の新生に対するプロタグランジンの作用

Papers 40

  1. Congenital Hypothyroidism and Brain Development: Association With Other Psychiatric Disorders Invited Peer-reviewed

    Katsuya Uchida, Mao Suzuki

    Frontiers in Neuroscience　15　2021/12/09

    Publisher: Frontiers Media SA

    DOI： 10.3389/fnins.2021.772382 　

    eISSN： 1662-453X

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    Thyroid hormones play an important role in brain development, and thyroid hormone insufficiency during the perinatal period results in severe developmental delays. Perinatal thyroid hormone deficiency is clinically known as congenital hypothyroidism, which is caused by dysgenesis of the thyroid gland or low iodine intake. If the disorder is not diagnosed or not treated early, the neuronal architecture is perturbed by thyroid hormone insufficiency, and neuropathological findings, such as abnormal synapse formation, defects in neuronal migration, and impairment of myelination, are observed in the brains of such patients. Furthermore, the expression of psychiatric disorder-related molecules, especially parvalbumin, is significantly decreased by thyroid hormone insufficiency during the perinatal period. Animal experiments using hypothyroidism models display decreased parvalbumin expression and abnormal brain architecture, and these experimental results show reproducibility and stability. These basic studies reinforce the results of epidemiological studies, suggesting the relevance of thyroid dysfunction in psychiatric disorders. In this review, we discuss the disruption of brain function associated with congenital hypothyroidism from the perspective of basic and clinical research.

  2. Thyroid hormone insufficiency alters the expression of psychiatric disorder-related molecules in the hypothyroid mouse brain during the early postnatal period International-journal Peer-reviewed

    Katsuya Uchida, Kentaro Hasuoka, Toshimitsu Fuse, Kenichi Kobayashi, Takahiro Moriya, Mao Suzuki, Norihiro Katayama, Keiichi Itoi

    Scientific Reports　11　(1)　1-10　2021/03/24

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-021-86237-8 　

    eISSN： 2045-2322

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    <title>Abstract</title>The functional role of thyroid hormone (TH) in the cortex and hippocampus of mouse during neuronal development was investigated in this study. TH insufficiency showed a decrease in the expression of parvalbumin (PV) in the cortex and hippocampus of pups at postnatal day (PD) 14, while treatment with thyroxine from PD 0 to PD 14 ameliorated the PV loss. On the other hand, treatment with antithyroid agents in adulthood did not result in a decrease in the expression of PV in these areas. These results indicate the existence of a critical period of TH action during the early postnatal period. A decrease in MeCP2-positive neuronal nuclei was also observed in the cortical layers II–IV of the cerebral cortex. The brains were then stained with CUX1, a marker for cortical layers II–IV. In comparison with normal mice, CUX1 signals were decreased in the somatosensory cortex of the hypothyroid mice, and the total thickness of cortical layers II–IV of the mice was lower than that of normal mice. These results suggest that TH insufficiency during the perinatal period strongly and broadly affects neuronal development.

  3. Female-Biased Sexual Dimorphism of Corticotropin-Releasing Factor Neurons in the Bed Nucleus of the Stria Terminalis International-journal Peer-reviewed

    Katsuya Uchida, Hiroko Otsuka, Masahiro Morishita, Shinji Tsukahara, Tatsuya Sato, Kenji Sakimura, Keiichi Itoi

    Biology of Sex Differences　10　(6)　1-11　2019/01

    DOI： 10.1186/s13293-019-0221-2 　

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    BACKGROUND: The bed nucleus of the stria terminalis (BNST) contains the highest density of corticotropin-releasing factor (CRF)-producing neurons in the brain. CRF-immunoreactive neurons show a female-biased sexual dimorphism in the dorsolateral BNST in the rat. Since CRF neurons cannot be immunostained clearly with available CRF antibodies in the mouse, we used a mouse line, in which modified yellow fluorescent protein (Venus) was inserted to the CRF gene, and the Neo cassette was removed, to examine the morphological characteristics of CRF neurons in the dorsolateral BNST. Developmental changes of CRF neurons were examined from postnatal stages to adulthood. Gonadectomy (GDX) was carried out in adult male and female mice to examine the effects of sex steroids on the number of CRF neurons in the dorsolateral BNST. METHODS: The number of Venus-expressing neurons, stained by immunofluorescence, was compared between male and female mice over the course of development. GDX was carried out in adult mice. Immunohistochemistry, in combination with Nissl staining, was carried out, and the effects of sex or gonadal steroids were examined by estimating the number of Venus-expressing neurons, as well as the total number of neurons or glial cells, in each BNST subnucleus, using a stereological method. RESULTS: Most Venus-expressing neurons co-expressed Crf mRNA in the dorsolateral BNST. They constitute a group of neurons without calbindin immunoreactivity, which makes a contrast to the principal nucleus of the BNST that is characterized by calbindin immunostaining. In the dorsolateral BNST, the number of Venus-expressing neurons increased across developmental stages until adulthood. Sexual difference in the number of Venus-expressing neurons was not evident by postnatal day 5. In adulthood, however, there was a significant female predominance in the number of Venus expressing neurons in two subnuclei of the dorsolateral BNST, i.e., the oval nucleus of the BNST (ovBNST) and the anterolateral BNST (alBNST). The number of Venus-expressing neurons was smaller significantly in ovariectomized females compared with proestrous females in either ovBNST or alBNST, and greater significantly in orchiectomized males compared with gonadally intact males in ovBNST. The total number of neurons was also greater significantly in females than in males in ovBNST and alBNST, but it was not affected by GDX. CONCLUSION: Venus-expressing CRF neurons showed female-biased sexual dimorphism in ovBNST and alBNST of the mouse. Expression of Venus in these subnuclei was controlled by gonadal steroids.

  4. Amelioration of improper differentiation of somatostatin-positive interneurons by triiodothyronine in a growth-retarded hypothyroid mouse strain Peer-reviewed

    Katsuya Uchida, Yusuke Taguchi, Chika Sato, Hidetaka Miyazaki, Kenichi Kobayashi, Tetsuya Kobayashi, Keiichi Itoi

    NEUROSCIENCE LETTERS　559　111-116　2014/01

    DOI： 10.1016/j.neulet.2013.11.052 　

    ISSN： 0304-3940

    eISSN： 1872-7972

  5. Stimulatory effects of prostaglandin E2 on neurogenesis in the dentate gyrus of the adult rat Peer-reviewed

    K Uchida, K Kumihashi, S Kurosawa, T Kobayashi, K Itoi, T Machida

    ZOOLOGICAL SCIENCE　19　(11)　1211-1216　2002/11

    DOI： 10.2108/zsj.19.1211 　

    ISSN： 0289-0003

  6. Acetylsalicylic acid reduces ischemia-induced proliferation of dentate cells in gerbils. Peer-reviewed

    Kentarou Kumihashi, Katsuya Uchida, Hidetaka Miyazaki, Junji Kobayashi, Takashi Tsuyshima, Takeo Machida

    Neuroreport　12　915-917　2001/01

    DOI： 10.1097/00001756-200104170-00010 　

  7. Long-lasting expression of FosB/ΔFosB immunoreactivity following acute stress in the paraventricular and supraoptic nuclei of the rat hypothalamus. International-journal

    Katsuya Uchida, Gopal Das, Ashraf H Talukder, Kazunori Kageyama, Keiichi Itoi

    Neuroscience research　217　104911-104911　2025/08

    DOI： 10.1016/j.neures.2025.104911 　

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    We examined expression profiles of FosB/∆FosB immunoreactivity and fosB gene transcripts in the paraventricular nucleus of the hypothalamus (PVH) and the supraoptic nucleus (SON) of rats following acute surgical stress (SS) and restraint stress (RS) and compared them with those of c-Fos immunoreactivity and c-fos mRNA. Following SS, the number of FosB/ΔFosB-ir cells markedly increased, the time course of which was slow-onset and long-lasting, in contrast with rapid-onset and short-lived c-Fos expression. Characteristically long-lasting FosB/ΔFosB expression was also observed following RS. On the other hand, fosB mRNA was short-lived, and its time course not much different from that of c-fos mRNA; thus, the long-lasting expression of FosB/∆FosB immunoreactivity may be attributed to the longer half-life of FosB proteins, and not to the persistent expression of fosB gene transcripts. Following SS, FosB/ΔFosB immunoreactivity was present mainly in PVH corticotropin-releasing factor (CRF) neurons and SON vasopressin (AVP) neurons, while c-Fos immunoreactivity in either PVH CRF neurons, or AVP and oxytocin neurons in PVH and SON. Following RS, FosB/ΔfosB- and c-Fos expression was almost restricted to PVH CRF neurons. The present study raises the possibility that FosB proteins in discrete populations of hypothalamic neuroendocrine neurons may play roles in forming adaptability to and/or resilience against stress, which takes longer than the acute phase response.

  8. Dual action of serotonin on local excitatory and inhibitory neural circuits regulating the corticotropin‐releasing factor neurons in the paraventricular nucleus of the hypothalamus

    Takayuki Sato, Takuma Sugaya, Ashraf Hossain Talukder, Yuki Tsushima, Shotaro Sasaki, Katsuya Uchida, Tatsuya Sato, Yoko Ikoma, Kenji Sakimura, Atsuo Fukuda, Ko Matsui, Keiichi Itoi

    Journal of Neuroendocrinology　35　(12)　2023/10/30

    Publisher: Wiley

    DOI： 10.1111/jne.13351 　

    ISSN： 0953-8194

    eISSN： 1365-2826

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    Abstract Serotonergic neurons originating from the raphe nuclei have been proposed to regulate corticotropin‐releasing factor (CRF) neurons in the paraventricular nucleus of the hypothalamus (PVH). Since glutamate‐ and γ‐aminobutyric acid (GABA)‐containing neurons, constituting the hypothalamic local circuits, innervate PVH CRF neurons, we examined whether they mediate the actions of serotonin (5‐hydroxytryptamine [5‐HT]) on CRF neurons. Spontaneous excitatory postsynaptic currents (sEPSCs) or spontaneous inhibitory postsynaptic currents (sIPSCs) were recorded in PVH CRF neurons, under whole cell patch‐clamp, using the CRF‐modified yellow fluorescent protein (Venus) ΔNeo mouse. Serotonin elicited an increase in the frequency of sEPSCs in 77% of the cells and a decrease in the frequency of sIPSCs in 71% of the cells, tested in normal medium. Neither the amplitude nor decay time of sEPSC and sIPSC was affected, thus the site(s) of action of serotonin may be presynaptic. In the presence of tetrodotoxin (TTX), serotonin had no significant effects on either parameter of sEPSC or sIPSC, indicating that the effects of serotonin are action potential‐dependent, and that the presynaptic interneurons are largely intact within the slice; distant neurons may exist, though, since some 20%–30% of neurons did not respond to serotonin without TTX. We next examined through what receptor subtype(s) serotonin exerts its effects on presynaptic interneurons. DOI (5‐HT2A/2C agonist) mimicked the action of serotonin on the sIPSCs, and the serotonin‐induced decrease in sIPSC frequency was inhibited by a selective 5‐HT2C antagonist RS102221. 8‐OH‐DPAT (5‐HT1A/7 agonist) mimicked the action of serotonin on the sEPSCs, and the serotonin‐induced increase in sEPSC frequency was inhibited by a selective 5‐HT7 antagonist SB269970. Thus, serotonin showed a dual action on PVH CRF neurons, by upregulating glutamatergic‐ and downregulating GABAergic interneurons; the former may partly be mediated by 5‐HT7 receptors, whereas the latter by 5‐HT2C receptors. The CRF‐Venus ΔNeo mouse was useful for the electrophysiological examination.

  9. Vasopressin Expressed in Hypothalamic CRF Neurons Causes Impaired Water Diuresis in Secondary Adrenal Insufficiency

    Satoshi Yamagata, Ashraf H Talukder, Shingo Murasawa, Kanako Niioka, Naoya Kumagai, Mao Takagi, Meiko Kawamura, Rie Natsume, Manabu Abe, Katsuya Uchida, Tatsuya Sato, Akira Kurose, Kazunori Kageyama, Makoto Daimon, Kenji Sakimura, Keiichi Itoi

    Endocrinology　164　(8)　2023/06/26

    Publisher: The Endocrine Society

    DOI： 10.1210/endocr/bqad109 　

    eISSN： 1945-7170

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    Abstract Patients with secondary adrenal insufficiency can present with impaired free water excretion and hyponatremia, which is due to the enhanced secretion of vasopressin (AVP) despite increased total body water. AVP is produced in magnocellular neurons in the paraventricular nucleus of the hypothalamus (PVH) and supraoptic nucleus and in parvocellular corticotropin-releasing factor (CRF) neurons in the PVH. This study aimed to elucidate whether magnocellular AVP neurons or parvocellular CRF neurons coexpressing AVP are responsible for the pathogenesis of hyponatremia in secondary adrenal insufficiency. The number of CRF neurons expressing copeptin, an AVP gene product, was significantly higher in adrenalectomized AVP-floxed mice (AVPfl/fl) than in sham-operated controls. Adrenalectomized AVPfl/fl mice supplemented with aldosterone showed impaired water diuresis under ad libitum access to water or after acute water loading. They became hyponatremic after acute water loading, and it was revealed under such conditions that aquaporin-2 (AQP2) protein levels were increased in the kidney. Furthermore, translocation of AQP2 to the apical membrane was markedly enhanced in renal collecting duct epithelial cells. Remarkably, all these abnormalities observed in the mouse model for secondary adrenal insufficiency were ameliorated in CRF-AVP−/− mice that lacked AVP in CRF neurons. Our study demonstrates that CRF neurons in the PVH are responsible for the pathogenesis of impaired water excretion in secondary adrenal insufficiency.

  10. Downstream projection of Barrington's nucleus to the spinal cord in mice Peer-reviewed

    Masahiro Kawatani, William deGroat, Keiichi Itoi, Katsuya Uchida, Kenji Sakimura, Akihiro Yamanaka, Takayuki Yamashita, Masahito Kawatani

    Journal of Neurophysiology　126　(6)　1959-1977　2021/11/03

    Publisher: American Physiological Society

    DOI： 10.1152/jn.00026.2021 　

    ISSN： 0022-3077

    eISSN： 1522-1598

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    Barrington's nucleus (Bar) which controls micturition behavior through downstream projections to the spinal cord contains two types of projection neurons BarCRH and BarESR1 that have different functions and target different spinal circuitry. Both types of neurons project to the L6-S1 spinal intermediolateral (IML) nucleus while BarESR1 neurons also project to the dorsal commissural nucleus (DCN). To obtain more information about the spinal circuits targeted by Bar, we used patch-clamp recording in spinal slices from adult mice in combination with optogenetic stimulation of Bar terminals. Recording of opto-evoked excitatory post synaptic currents (oEPSCs) in DiI-labeled lumbosacral preganglionic neurons (LS-PGN) revealed that both Bar neuronal populations make strong glutamatergic monosynaptic connections with LS-PGN, while BarESR1 neurons also elicited smaller amplitude glutamatergic polysynaptic oEPSCs or polysynaptic inhibitory post synaptic currents (oIPSCs) in some LS-PGN. Optical stimulation of BarCRH and BarESR1 terminals also elicited monosynaptic oEPSCs and polysynaptic oIPSCs in sacral DCN neurons, some of which must include interneurons projecting either to the IML or ventral horn. Application of capsaicin increased opto-evoked firing during repetitive stimulation of Bar terminals through the modulation of spontaneous post synaptic currents in LS-PGN. In conclusion, our experiments have provided insights into the synaptic mechanisms underlying the integration of inputs from Bar to autonomic circuitry in the lumbosacral spinal cord that may control micturition.

  11. Cholinergic modulation of CRH and non-CRH neurons in Barrington’s nucleus of the mouse Peer-reviewed

    Masahiro Kawatani, Keiichi Itoi, Ashraf Hossain Talukder, Katsuya Uchida, Kenji Sakimura, Masahito Kawatani

    Journal of Neurophysiology　124　(2)　443-457　2020/08/01

    Publisher: American Physiological Society

    DOI： 10.1152/jn.00342.2019 　

    ISSN： 0022-3077

    eISSN： 1522-1598

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    This study investigates the effects of bath-applied cholinergic agonists on Barrington’s nucleus (BarN) neurons in vitro. They were either excitatory, through nicotinic receptors, or inhibitory, through muscarinic receptors. Putative corticotropin-releasing hormone (CRH)-expressing neurons in BarN, as well as putative non-CRH-expressing neurons, responded to cholinergic agonists.

  12. Correction to: Female-biased sexual dimorphism of corticotropin-releasing factor neurons in the bed nucleus of the stria terminalis. International-journal

    Katsuya Uchida, Hiroko Otsuka, Masahiro Morishita, Shinji Tsukahara, Tatsuya Sato, Kenji Sakimura, Keiichi Itoi

    Biology of sex differences　10　(1)　10-10　2019/02/19

    DOI： 10.1186/s13293-019-0224-z 　

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    Following publication of the original article [1], we noticed a number of errors.

  13. Genome-Wide Analysis of Glucocorticoid-Responsive Transcripts in the Hypothalamic Paraventricular Region of Male Rats. International-journal Peer-reviewed

    Keiichi Itoi, Ikuko Motoike, Ying Liu, Sam Clokie, Yasumasa Iwasaki, Katsuya Uchida, Tatsuya Sato, Greti Aguilera

    Endocrinology　160　(1)　38-54　2019/01/01

    DOI： 10.1210/en.2018-00535 　

    ISSN： 0013-7227

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    Glucocorticoids (GCs) are essential for stress adaptation, acting centrally and in the periphery. Corticotropin-releasing factor (CRF), a major regulator of adrenal GC synthesis, is produced in the paraventricular nucleus of the hypothalamus (PVH), which contains multiple neuroendocrine and preautonomic neurons. GCs may be involved in diverse regulatory mechanisms in the PVH, but the target genes of GCs are largely unexplored except for the CRF gene (Crh), a well-known target for GC negative feedback. Using a genome-wide RNA-sequencing analysis, we identified transcripts that changed in response to either high-dose corticosterone (Cort) exposure for 12 days (12-day high Cort), corticoid deprivation for 7 days (7-day ADX), or acute Cort administration. Among others, canonical GC target genes were upregulated prominently by 12-day high Cort. Crh was upregulated or downregulated most prominently by either 7-day ADX or 12-day high Cort, emphasizing the recognized feedback effects of GC on the hypothalamic-pituitary-adrenal (HPA) axis. Concomitant changes in vasopressin and apelin receptor gene expression are likely to contribute to HPA repression. In keeping with the pleotropic cellular actions of GCs, 7-day ADX downregulated numerous genes of a broad functional spectrum. The transcriptome response signature differed markedly between acute Cort injection and 12-day high Cort. Remarkably, six immediate early genes were upregulated 1 hour after Cort injection, which was confirmed by quantitative reverse transcription PCR and semiquantitative in situ hybridization. This study may provide a useful database for studying the regulatory mechanisms of GC-dependent gene expression and repression in the PVH.

  14. Behavioral testing and resting-state brain functional connectivity analysis of the methimazole-induced transient hypothyroidism mice Peer-reviewed

    Yuto Yoshida, Ryusei Uchida, Katsuya Uchida, Keiichi Itoi, Mitsuyuki Nakao, Norihiro Katayama

    IEICE Technical Report　MBE2018-33　31　2018/10

  15. golgi method Invited Peer-reviewed

    UCHIDA Katsuya

    https://bsd.neuroinf.jp/wiki/ゴ　2018/04

    DOI： 10.14931/bsd.7511 　

  16. Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse Peer-reviewed

    Junko Kono, Kohtarou Konno, Ashraf Hossain Talukder, Toshimitsu Fuse, Manabu Abe, Katsuya Uchida, Shuhei Horio, Kenji Sakimura, Masahiko Watanabe, Keiichi Itoi

    BRAIN STRUCTURE & FUNCTION　222　(4)　1705-1732　2017/05

    DOI： 10.1007/s00429-016-1303-0 　

    ISSN： 1863-2653

    eISSN： 1863-2661

  17. Visualization of Corticotropin-Releasing Factor Neurons by Fluorescent Proteins in the Mouse Brain and Characterization of Labeled Neurons in the Paraventricular Nucleus of the Hypothalamus Peer-reviewed

    Keiichi Itoi, Ashraf Hossain Talukder, Toshimitsu Fuse, Takuji Kaneko, Ryo Ozawa, Takayuki Sato, Takuma Sugaya, Katsuya Uchida, Maya Yamazaki, Manabu Abe, Rie Natsume, Kenji Sakimura

    ENDOCRINOLOGY　155　(10)　4054-4060　2014/10

    DOI： 10.1210/en.2014-1182 　

    ISSN： 0013-7227

    eISSN： 1945-7170

  18. Selective Ablation of Dopamine Beta-Hydroxylase Neurons (Subpopulation of TH Neurons) in the Brain: New Insights into Brain Catecholaminergic Circuitry and Catecholamine-Related Diseases Peer-reviewed

    Keiichi Itoi, Naoya Sugimoto, Katsuya Uchida, Toshimitsu Fuse, Gopal Das, Shinji Ohara, Kazuto Kobayashi

    Catecholamine Research in the 21st Century: Abstracts and Graphical Abstracts, 10th International Catecholamine Symposium, 2012　27-27　2013/12

    Publisher: Elsevier Inc.

    DOI： 10.1016/B978-0-12-800044-1.00023-4 　

  19. Surgical effects on soft tissue produced by a 405-nm violet diode laser in vivo Peer-reviewed

    H. Miyazaki, J. Kato, S. Kawai, H. Hatayama, K. Uchida, M. Otsuki, J. Tagami, S. Yokoo

    LASER PHYSICS　21　(12)　2128-2131　2011/12

    DOI： 10.1134/S1054660X11210158 　

    ISSN： 1054-660X

  20. バソプレシンおよびオキシトシンの中枢作用と創薬ターゲツトとしてのバソプレシン受容体, オキシトシン受容体の意義 Invited Peer-reviewed

    井樋慶一, 布施俊光, 内田克哉

    1　(2)　134-141　2011/10

  21. Targeting of Locus Ceruleus Noradrenergic Neurons Expressing Human Interleukin-2 Receptor alpha-Subunit in Transgenic Mice by a Recombinant Immunotoxin anti-Tac(Fv)-PE38: A Study for Exploring Noradrenergic Influence upon Anxiety-Like and Depression-Like Behaviors Peer-reviewed

    Keiichi Itoi, Naoya Sugimoto, Saya Suzuki, Keisuke Sawada, Gopal Das, Katsuya Uchida, Toshimitsu Fuse, Shinji Ohara, Kazuto Kobayashi

    JOURNAL OF NEUROSCIENCE　31　(16)　6132-6139　2011/04

    DOI： 10.1523/JNEUROSCI.5188-10.2011 　

    ISSN： 0270-6474

  22. Identification of novel transcription factors for catecholamine gene expression based upon the comprehensive analyses of transcripts in fetal pontine noradrenergic neurons using trasngenic mice expressing green fluorescent protein under the promoter of tyrosine hydroxylase Peer-reviewed

    Haruka Yokohashi, Katsuya Uchida, Toshimitsu Fuse, Yasumasa Iwasaki, Kengo Kinoshita, Akira Okazaki, Gopal Das, Keisuke Sawada, Chiaki Tanaka, Hiroaki Tomita, Shinji Ohara, Kazuto Kobayashi, Keiichi Itoi

    NEUROSCIENCE RESEARCH　71　E120-E120　2011

    Publisher: ELSEVIER IRELAND LTD

    DOI： 10.1016/j.neures.2011.07.514 　

  23. 出血性低血圧ショックストレス時の視床下部室傍核活性化における延髄プロラクチン放出ペプチド含有ニューロンの関与 Peer-reviewed

    内田克哉, 小林大輔, ゴーパルダス, 尾仲達史, 井上金治, 井樋慶一

    ACTH RELATED PEPTIDES　21　25-27　2010/03

    Publisher:

    ISSN： 1340-4512

  24. Participation of the Prolactin-Releasing Peptide-Containing Neurones in Caudal Medulla in Conveying Haemorrhagic Stress-Induced Signals to the Paraventricular Nucleus of the Hypothalamus Peer-reviewed

    K. Uchida, D. Kobayashi, G. Das, T. Onaka, K. Inoue, K. Itoi

    JOURNAL OF NEUROENDOCRINOLOGY　22　(1)　33-42　2010/01

    DOI： 10.1111/j.1365-2826.2009.01935.x 　

    ISSN： 0953-8194

  25. Glucocorticoid Dependency of Surgical Stress-Induced FosB/Delta FosB Expression in the Paraventricular and Supraoptic Nuclei of the Rat Hypothalamus Peer-reviewed

    G. Das, K. Uchida, K. Kageyama, Y. Iwasaki, T. Suda, K. Itoi

    JOURNAL OF NEUROENDOCRINOLOGY　21　(10)　822-831　2009/10

    DOI： 10.1111/j.1365-2826.2009.01902.x 　

    ISSN： 0953-8194

  26. Suppression of immunoreactive FosB/delta FosB by glucocorticoid in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of the rat hypothlamus. Peer-reviewed

    Das G, Uchida K, Itoi K

    ACTH Related Peptides　19　47-51　2009

  27. 甲状腺機能低下による大脳皮質および海馬における神経学的異常 Peer-reviewed

    内田 克哉, 小林 哲也, 町田 武生, 井樋 慶一

    日本内分泌学会雑誌　84　(2)　542-542　2008/09

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  28. Quantitative analysis of thyroid-stimulating hormone messenger RNA and heterogeneous nuclear RNA in hypothyroid rats Peer-reviewed

    Koreaki Sugimoto, Kouki Mori, Katsuva Uchida, Daisuke Kobayashi, Keiichi Itoi

    BRAIN RESEARCH BULLETIN　74　(1-3)　142-146　2007/09

    DOI： 10.1016/j.brainresbull.2007.06.004 　

    ISSN： 0361-9230

  29. Modulatory action of prolactin-releasing peptide on norepinephrine-induced activation of neuroendocrine neurons in the hypothalamus. Peer-reviewed

    Itoi K, Onaka T, Kobayashi D, Uchida K, Hamaoka S, Suzuki S, Nakamura H, Ishii Y, Das G

    ACTH Related Peptides　(17)　33-36　2006

  30. Topographic analysis of cell proliferation in the hippocampus of the adult mouse Peer-reviewed

    K Uchida, S Nakamura, T Kobayashi, T Machida

    NEUROREPORT　16　(18)　2033-2036　2005/12

    DOI： 10.1097/00001756-200512190-00013 　

    ISSN： 0959-4965

  31. Impaired neurogenesis in the growth-retarded mouse is reversed by T-3 treatment Peer-reviewed

    K Uchida, M Yonezawa, S Nakamura, T Kobayashi, T Machida

    NEUROREPORT　16　(2)　103-106　2005/02

    DOI： 10.1097/00001756-200502080-00005 　

    ISSN： 0959-4965

  32. 出血ショック時、視床下部室傍核活性化へのプロラクチン放出ペプチドニューロンの関与. Invited

    井樋慶一, 小林大輔, 内田克哉

    ACTH Related Peptides　(16)　103-110　2005

  33. Differential effects of forced swim-stress on the corticotropin-releasing hormone and vasopressin gene transcription in the parvocellular division of the paraventricular nucleus of rat hypothalamus Peer-reviewed

    YQ Jiang, H Kawashima, Y Iwasaki, K Uchida, K Sugimoto, K Itoi

    NEUROSCIENCE LETTERS　358　(3)　201-204　2004/04

    DOI： 10.1016/j.neulet.2004.01.041 　

    ISSN： 0304-3940

  34. 脱血ショックによるストレス情報を視床下部に伝達する神経路の検討. Peer-reviewed

    小林大輔, 内田克哉, 井樋慶一

    ACTH Related Peptides　2004

    ISSN： 1340-4512

  35. The Present Research on Adult Neurogenesis and Neural Stem Cells. Invited Peer-reviewed

    内田克哉

    SUNDAI FORUM　(22)　175-187　2004

  36. 成長遅延症マウス海馬歯状回における甲状腺ホルモンによる神経新生 Peer-reviewed

    米沢 昌子, 内田 克哉, 小林 哲也, 町田 武生

    日本内分泌学会雑誌　79　(2)　379-379　2003/09

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  37. Regulation of corticotropin releasing hormone receptor (CRH-R) in the rat anterior pituitary as assessed by radioimmunoassay Invited Peer-reviewed

    Takeshi Nigawara, Nobuo Horiba, Fumiko Tozawa, Yoko Kosagi, Katsuya Uchida, Yasumasa Iwasaki, Toshihiro Suda

    Pituitary　6　(2)　67-73　2003

    DOI： 10.1023/B:PITU.0000004796.71734.e4 　

    ISSN： 1386-341X

  38. Basic properties and annual changes of follicle-stimulating hormone receptors in the testis of horseshoe bats, Rhinolophus ferrumequinum Peer-reviewed

    T Hayashi, K Uchida, K Kawamoto

    JOURNAL OF EXPERIMENTAL ZOOLOGY　292　(3)　304-313　2002/02

    DOI： 10.1002/jez.10012 　

    ISSN： 0022-104X

  39. プロスタグランジンE2の歯状回細胞増殖に対する効果 Peer-reviewed

    内田 克哉, 組橋 堅太郎, 黒沢 怜史, 町田 武生

    神経化学　40　(2-3)　457-457　2001/09

    Publisher: 日本神経化学会

    ISSN： 0037-3796

  40. Pyrogens: Techniques of Research into Fever-inducing Agents. Peer-reviewed

    内田克哉

    SUNDAI FORUM　(16)　51-64　1998

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Misc. 30

  1. 第44回大会実行委員主催シンポジウム「腸内環境とホメオスタシス」開催記 Invited Peer-reviewed

    Takafumi Sakai, Kastsuya Uchida

    Comparative Endocrinology　46　(169)　36-37　2020

    Publisher: Japan Society for Comparative Endocrinology

    DOI： 10.5983/nl2008jsce.46.36 　

    ISSN： 1882-6636

    eISSN： 1882-6644

  2. Behavioral testing and resting-state brain functional connectivity analysis of the methimazole-induced transient hypothyroidism mice

    吉田侑冬, 吉田侑冬, 内田竜生, 内田克哉, 井樋慶一, 中尾光之, 片山統裕

    電子情報通信学会技術研究報告　118　(257(MBE2018 27-35)(Web))　31‐36 (WEB ONLY)　2018/10/13

    ISSN： 0913-5685

  3. 視床下部コルチコトロピン放出因子(CRF)ニューロンを調節する神経性入力の同定

    杉原史章, 籠谷勇人, 佐々木昭太郎, 内田克哉, 佐藤達也, 松井広, 崎村建司, 井樋慶一

    日本下垂体研究会学術集会プログラム・講演要旨集　33rd　50　2018/09

  4. 分界条床核コルチコトロピン放出因子含有ニューロンの性差

    内田克哉, 大塚寛子, 森下雅大, 塚原伸治, 佐藤達也, 井樋慶一

    日本内分泌学会雑誌　94　(1)　371-371　2018/04/01

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  5. マウス甲状腺機能低下症モデルの大脳皮質感覚野におけるmethyl‐CpG binding protein2発現低下に関する報告

    内田克哉, 蓮岡健太郎, 守屋孝洋, 小林健一, 井樋慶一

    日本内分泌学会雑誌　94　(4 (Web))　1226(J‐STAGE)　2018

    ISSN： 0029-0661

  6. クレチン病モデルマウスにおける体内時計の機能異常と甲状腺ホルモン補充療法による改善効果

    蓮岡健太郎, 内田克哉, 對馬千沙都, 對馬千沙都, 井上莉香, 井上莉香, 木村隼也, 木村隼也, 井樋慶一, 平澤典保, 守屋孝洋, 守屋孝洋

    日本薬理学会北部会プログラム・抄録集　68th　39　2017

  7. 脳発達期の一時的な甲状腺ホルモン欠乏がもたらすパルブアルブミン遺伝子発現と概日リズムへの影響

    内田克哉, 井上梨香, 蓮岡健太郎, 守屋孝洋, 佐藤達也, 井樋慶一

    日本内分泌学会雑誌　93　(4 (Web))　1166(J‐STAGE)　2017

    ISSN： 0029-0661

  8. 副腎不全に伴う視床下部室傍核CRFニューロンにおけるバゾプレシン発現について

    山形聡, TALUKDER Ashraf Hossain, 内田克哉, 佐藤達也, 夏目里恵, 阿部学, 崎村建司, 井樋慶一

    バゾプレシン研究会プログラム・講演抄録　27th　23　2016/12/01

  9. 分界条床核コルチコトロピン放出因子(CRF)産生ニューロンの性差―CRF‐VenusΔNeoマウスを用いた検討

    大塚寛子, 内田克哉, 布施俊光, 崎村建司, 井樋慶一

    日本神経内分泌学会学術集会プログラム・抄録集　43rd　66　2016

  10. 幼若期の甲状腺ホルモン欠乏はパルブアルブミン発現を抑制し自発運動量の変化を誘発する

    内田克哉, 井上莉香, 守屋孝洋, 布施俊光, 井樋慶一

    日本神経内分泌学会学術集会プログラム・抄録集　43rd　66　2016

  11. 脳発達期の甲状腺ホルモン欠乏はマウスの大脳皮質および海馬歯状回におけるパルブアルブミン陽性GABAニューロンの発達を遅延させる

    内田克哉, 小林健一, 布施俊光, 井樋慶一

    日本内分泌学会雑誌　91　(2 Suppl.)　97　2015/11/20

    ISSN： 0029-0661

  12. CRF‐VenusΔNeoノックインマウスを用いたCRF含有ニューロンの個体発生とその脳内分布の神経解剖学的解析

    大塚寛子, 内田克哉, 布施俊光, 崎村建司, 井樋慶一, 井樋慶一

    日本内分泌学会雑誌　91　(2)　526　2015/09/20

    ISSN： 0029-0661

  13. 視床下部コルチコトルピン放出因子(CRF)ニューロンへの抑制性入力とセロトニン(5‐HT)による制御機序に関する研究

    佐藤隆幸, 菅谷琢磨, 福田敦夫, 杉本直哉, 布施俊光, 内田克哉, 阿部学, 山崎真弥, 崎村建司, 井樋慶一, 井樋慶一

    日本内分泌学会雑誌　91　(2)　526　2015/09/20

    ISSN： 0029-0661

  14. バソプレシンおよびオキシトシンの中枢作用と創薬ターゲットとしてのバソプレシン受容体、オキシトシン受容体の意義

    井樋慶一, 布施俊光, 内田克哉

    Fluid Management Renaissance　1　(2)　28-33　2011/10/01

    Publisher: メディカルレビュー社

  15. Selective ablation of locus coeruleus noradrenergic neurons expressing human interleukin-2 receptor alpha-subunit in transgenic mice by a recombinant immunotoxin: Exploring noradrenergic influence upon anxiety-like and depression-like behaviors

    Keiichi Itoi, Naoya Sugimoto, Saya Suzuki, Keisuke Sawada, Gopal Das, Katsuya Uchida, Toshimitsu Fuse, Shinji Ohara, Kazuto Kobayashi

    NEUROSCIENCE RESEARCH　71　E188-E188　2011

    DOI： 10.1016/j.neures.2011.07.812 　

    ISSN： 0168-0102

  16. Gamma-aminobutyric acid interneuron hypoplasia in the hypothyroidal growth-retarded mouse brain is improved by triiodothyronine treatment at early stage of the postnatal life

    Katsuya Uchida, Yusuke Taguchi, Chika Sato, Hidetaka Miyazaki, Toshimitsu Fuse, Tetsuya Kobayashi, Keiichi Itoi

    NEUROSCIENCE RESEARCH　71　E398-E399　2011

    DOI： 10.1016/j.neures.2011.07.1747 　

    ISSN： 0168-0102

  17. 出血性低血圧ショックストレス時の視床下部室傍核(PVN)活性化における延髄プロラクチン放出ペプチド(PrRP)含有ニューロンの関与(Participation of the Prolactin-Releasing Peptide-Containing Neurones in Caudal Medulla in Conveying Haemorrhagic Stress-Induced Signals to the Paraventricular Nucleus of the Hypothalamus)

    内田 克哉, 小林 大輔, ダス・ゴーパル, 尾仲 達史, 井上 金治, 井樋 慶一

    神経化学　49　(2-3)　650-650　2010/08

    Publisher: 日本神経化学会

    ISSN： 0037-3796

  18. Participation of the prolactin-releasing peptide-containing neurons in caudal medulla in conveying hemorrhagic stress-induced signals to the paraventricular nucleus of the hypothalamus

    Keiichi Itoi, Katsuya Uchida, Gopal Das, Kinji Inoue, Tatsushi Onaka

    ENDOCRINE JOURNAL　57　S538-S538　2010/03

    ISSN： 0918-8959

  19. Participation of the prolactin-releasing peptide-containing neurones in caudal medulla in conveying haemorrhagic stress-induced signals to the paraventricular nucleus of the hypothalamus

    Katsuya Uchida, Daisuke Kobayashi, Gopal Das, Tatsushi Onaka, Kinji Inoue, Keiichi Itoi

    NEUROSCIENCE RESEARCH　68　E282-E282　2010

    DOI： 10.1016/j.neures.2010.07.1255 　

    ISSN： 0168-0102

  20. The role of glucocorticoid on neuronal FosB/Delta FosB-Iike immunoreactivity in the rat brain following adrenalectomy

    Gopal Das, Katsuya Uchida, Keiichi Itoi

    NEUROSCIENCE RESEARCH　61　S188-S188　2008

    ISSN： 0168-0102

  21. Long-term effects of voluntary running on neurogenesis of adult mice

    Katsuya Uchida, Keiichi Itoi

    NEUROSCIENCE RESEARCH　58　S209-S209　2007

    ISSN： 0168-0102

  22. 「脳の性分化」（山内兄人 新井康充 著 裳華房） Peer-reviewed

    内田 克哉

    生物科学ニュース　(5)　2006/05

  23. SELECTIVE COLLECTION OF CATECHOLAMINERGIC (CA) NEURONS IN THE BRAIN AND ITS APPLICATION TO FUNCTIONAL ANALYSES USING TYROSINE HYDROXYLASE (TH)-GREEN FLUORESCENT PROTEIN (GFP) TRANSGENIC MICE

    NAKAMURA Hiroaki, ISHII Yoshiyuki, UCHIDA Katsuya, HOMMA Yu, KOBAYASHI Kazuto, ITOI Keiichi

    (20)　40-40　2005/11/12

    ISSN： 0913-9036

  24. In vivo dynamics of TSH beta gene transcription in hypothyroidism by quantitative real-time PCR

    K Sugimoto, D Kobayashi, K Uchida

    JOURNAL OF PSYCHOSOMATIC RESEARCH　58　(6)　S60-S60　2005/06

    ISSN： 0022-3999

  25. 「カフェインの科学 -コーヒー，茶，チョコレートの薬理作用」 （栗原 久著 学会出版センター）

    内田 克哉

    日本家政学会誌　55　(3)　644-644　2005/03

    Publisher: 日本家政学会

  26. Effects of prostaglandin receptor stimuli on neurogenesis in the dentate gyrus of the adult rat

    K Uchida, K Itoi

    JOURNAL OF PHARMACOLOGICAL SCIENCES　94　55P-55P　2004

    ISSN： 1347-8613

  27. NEUROGENESIS OF HIPPOCAMPAL CELLS BY THYROID HORMONE IN THE HYPOTHYROIDAL MUTANT MOUSE(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Yonezawa Masako, Kumihashi Kentarou, Uchida Katsuya, Kobayashi Tetsuya, Machida Takeo

    Zoological science　20　(12)　1582-1582　2003

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  28. EFFECTS OF PROSTAGLANDIN RECEPTOR STIMULI ON NEUROGENESIS IN THE DENTATE GYRUS OF THE ADULT RAT(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Uchida Katsuya, Machida Takeo, Itoi Keiichi

    Zoological science　20　(12)　1588-1588　2003

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  29. HEMORRHAGIC SHOCK-INDUCED PHOSPHORYLATION OF CRE-BINDING PROTEIN IN THE RAT BRAIN(Endocrinology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Kobayashi Daisuke, Uchida Katsuya, Machida Takeo, Jang Yaqiu, Kawashima Hisayoshi, Sugimoto Koreaki, Isono Kunio, Itoi Keiichi

    Zoological science　20　(12)　1598-1599　2003

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  30. Prostaglandin E2 increases the proliferation of granule cell precursors in the dentate gyrus of the adult rat(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Kumihashi Kentarou, Uchida Katsuya, Machida Takeo

    Zoological science　18　98-98　2001

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

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Books and Other Publications 3

  1. ヒト扁桃体研究ハンドブック

    章, 井樋慶一, 内田克哉, 布施俊光, 章, 内田克哉, 井樋慶一, 坂本敏郎

    西村書店　2015/09

    ISBN: 9784890134557

  2. Progress in Cell Growth Process Research

    Uchida K

    Nova Science Publishers Inc　2008/08

    ISBN: 9781604563252

    More details Close

    in press

  3. Neurogenesis Research Advances

    Uchida K, Kobayashi T, Machida T

    Nova Science Publishers Inc　2008/04/01

    ISBN: 1600216765

Presentations 8

  1. マウス甲状腺機能低下症モデルの大脳皮質感覚野におけるmethyl-CpG binding protein 2発現低下に関する報告

    内田 克哉, 蓮岡健太郎, 守屋孝洋, 小林健一, 井樋慶一

    日本甲状腺学会　2018/11/23

  2. メチマゾール誘発一過性甲状腺機能低下症マウスの行動試験と安静時脳機能結合の解析

    吉田侑冬, 内田竜生, 内田克哉, 井樋慶一, 中尾光之, 片山統裕

    電子情報通信学会　2018/10/20

  3. 幼若期の甲状腺ホルモンの欠乏はマウス大脳皮質感覚野における methyl-CpG binding protein 2 発現を低下させる

    内田 克哉, 蓮岡, 健太郎, 守屋, 孝洋, 小林, 健一, 井樋 慶一

    日本動物学会第８９回札幌大会　2018/10/09

  4. シンポジウム；神経・内分泌システムにおける細胞の運命制御

    UCHIDA Katsuya

    日本動物学会 第８４回岡山大会　2014/09/26

  5. シンポジウム：哺乳動物の脳神経系およびホルモン研究に関する最近の話題

    第81回日本動物学会 東京大会　2010/09/23

  6. シンポジウム：脳の病態とプロスタグランジン 成体海馬におけるプロスタグランジンの役割 Invited

    内田 克哉, 井樋 慶一

    第７７回日本薬理学会年会　2004/03/10

  7. 「海馬神経細胞の新生を制御する物質 -特にプロスタグランディンについて-」 Invited

    Katsuya Uchida

    東京都老人総合研究所 運動・自律機能相関研究グループセミナー　2003/04

  8. 「脳の神経細胞の新生を促進する物質と抑制する物質」 Invited

    Uchida Katsuya

    東北大学電気通信研究所講演会　2002/03

Show all Show first 5

Research Projects 21

  1. 全環境トランスクリプトームの種間比較による実験動物適用限界の明瞭化

    大林 武, 内田 克哉

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2023/04/01 - 2026/03/31

    More details Close

    本研究では、比較トランスクリプトーム解析を用いて実験動物の適用限界を明瞭化する。トランスクリプトームの種間比較を行うには、生物種を超えた遺伝子の対応付けだけでなく、サンプルの対応付けも必要である。異なる生物種では、遺伝子の種類、各組織における遺伝子発現、器官を構成する組織の割合、個体の成長速度など、多くの相違が存在する。これらの階層的な相違を考慮に入れるため、本研究では各生物種の発現量データを種間比較可能な形式に変換して解析する。ヒト、マウス、ラットを対象に、公共レポジトリから得たRNAseqデータを基に遺伝子発現データを構築し、主成分分析を用いてサンプルの線形変換を行った結果、合成されたメタサンプルの寄与率が冪乗的に減衰することが観察された。この冪乗的減衰は生物学的に本質的な特性である可能性がある一方で、データ処理の不完全さが原因である可能性もあるため、RNAseqデータから遺伝子発現量データを導出するマッピング方法について検討を行った。特に、生物種間で遺伝子が多対多に対応している場合、マッピング方法が遺伝子共発現関係に有意な影響を与えることが明らかになった。 並行して、比較トランスクリプトーム法の検証として、脳における遺伝子発現と機能の解析方法の高速化を行った。In situ hybridization法や免疫染色法は、組織上での遺伝子やタンパク質の発現を可視化する重要なツールであるが、大量の遺伝子について繰り返し測定を行うには時間がかかる。そこで、電解攪拌技術を応用し、反応時間を短縮する試みを行った結果、免疫染色法においては、従来2日間を要していた工程を2時間以内に短縮することができ、大量解析の可能性が示された。

  2. Development and application of optical imaging measurement of brain metabolite clearance characteristics

    Katayama Norihiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    2019/04/01 - 2022/03/31

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    This study modified the functional integrative optical imaging (fIOI) method developed by the principal investigators to quantify temporal changes in brain clearance characteristics to improve temporal resolution and signal-to-noise ratio. The improved method was applied to in vivo mice that spontaneously repeat sleep-wake states to quantify the sleep state dependence. The results showed that clearance was more rapid during the sleep phase than during the wake phase. This result is similar to previous reports using real-time micro-iontophoresis. The fIOI experimental system was mathematically modeled, and numerical simulations revealed some of the limitations of the performance of this experimental system.

  3. Involvement of Thyroid Hormones in Integrative Brain Function -Relevance for Psychiatric Disorders-

    Category: Multifunctional electrodes and other physiological tools

    2021/07 - 2022/03

  4. 統合的脳機能を支える甲状腺ホルモンの精神基盤構築への接点 Competitive

    内田 克哉

    Offer Organization: 日本学術振興会

    System: 科学研究補助金基盤研究C

    2019/04 - 2022/03

  5. Functional diversity of the subnuclei of PVH CRF neurons

    Itoi Keiichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    2018/04/01 - 2021/03/31

    More details Close

    We discovered that corticotropin-releasing factor (CRF) neurons in the paraventricular nucleus of the hypothalamus (PVH) project to multiple brain regions besides the median eminence, and that the cell bodies of the PVH CRF neurons comprises multiple subnuclei. We injected retrograde viral vector HiRet to the respective primary projection area, using the CRF-iCreDeltaNeo mouse, and elucidated the topographical characteristics of each subnucleus. Furthermore, functional roles of each subnucleus were uncovered by optogenetic or chemogenetic methods.

  6. 不快情動の脳内情報伝達中継核である分界条床核外側核におけるコルチコトロピン放出因子発現の性差とその生物学的意義 Competitive

    内田 克哉

    Offer Organization: 東北大学大学院情報科学研究科

    System: 情報科学研究科教育研究充実経費

    2018/10 - 2019/03

  7. 行動発現の神経基盤を制御する甲状腺ホルモン作用の新機軸 Competitive

    内田 克哉

    Offer Organization: 日本学術振興会

    System: 科学研究補助金基盤研究C

    2016/04 - 2019/03

  8. 不快情動の情報伝達における性スペクトラム 光遺伝学的手法によるコルチコトロピン放出ホルモン産生ニューロンの人為的制御からの検討 Competitive

    内田 克哉

    Offer Organization: 東北大学大学院情報科学研究科

    System: 情報科学研究科教育研究充実経費

    2017/10 - 2018/03

  9. 幼若期の一時的な内部環境破綻が成熟個体の大脳皮質活動パターンにもたらす影響とその病理-分子組織学的手法とマクロイメージング法の融合- Competitive

    内田 克哉

    Offer Organization: 東北大学大学院情報科学研究科

    System: 「学際的研究プロジェクト」開拓支援経費

    2017/04 - 2018/03

  10. ストレスによるCRF神経細胞のシナプス再構成―蛍光可視化マウスを用いた検討

    布施 俊光, 井樋 慶一, 内田 克哉

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2015/04/01 - 2017/03/31

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    ストレスに対する脳・神経系の応答は個体の機能維持のみならずヒトの行動，ひいてはヒトの社会活動にも影響をおよぼす重要な生体システムであり，その破綻は重大な結果をもたらすと考えられる．しかしながらその破綻機構の詳細は未だ解明されておらず，その分子メカニズムの解明はその予防，破綻の解消に繋がると期待される． 分子メカニズムの解明のため，研究代表者等によって開発されたストレス応答の中枢である視床下部コルチコトロピン放出因子神経細胞の蛍光可視化マウス(Itoi et al., 2014)を用いて，ストレス負荷にともなって同神経細胞のシナプスの形態や分子局在の再構築機構に迫る研究をおこなった． 平成２８年度は，前年度に引き続いて１．マウス個体を用いた慢性多様ストレス負荷の実験を行い，２．可視化されたシナプスタンパク質について分布を観察した．さらに，用いている視床下部コルチコトロピン放出因子分泌神経細胞可視化マウス(CRF-Venus)の改良型(CRF-VenusΔNeo)について詳細な解剖学的検討をおこない，視床下部以外の脳の領域においてもCRFがストレス応答について関与している可能性を検討した(Kono et al., 2016 accepted)．これらの結果，用いているCRF-Venusマウスの有用性が確認されるとともに，視床下部におけるストレス応答破綻メカニズムの検討が進んだ．CRF-Venusマウスの有用性については国内外の研究者に注目されており，多くの共同研究に発展したことからこの分野の研究が今後一層発展することが期待される．

  11. Exploring the regulatory mechanism of hypothalamic local circuitry using a mouse line for visualizing CRF neurons by a fluorescent protein

    Itoi Keiichi, WAKAMORI MINORU, SAKIMURA KENJI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    Hypothalamic local circuitry was examined using the CRF-Venus mouse in which CRF was labeled by modified yellow fluorescent protein. Hypothalamic CRF neurons receive direct glutamatergic and GABAergic inputs, and the glutamatergic EPSC frequency was increased by noradrenaline (NA). Thus, NA acitivates CRF neurons via glutamatergic interneurons in the hypothalamus. The Neo cassette was deleted from the genome of the CRF-Venus mouse, and CRF-VenusDeltaNeo mouse was generated. Venus fluorescent intensity, as well as its specificity for CRF neurons, was much improved. The distribution of CRF neurons in the whole brain of the mouse was disclosed for the first time using the CRF-VenusDeltaNeo.

  12. 不快情動の情報伝達には性的な差が認められるか?分界条床核におけるコルチコトロピン放出ホルモン産生ニューロンの性差からの検討 Competitive

    内田 克哉

    Offer Organization: 東北大学大学院情報科学研究科

    System: 情報科学研究科教育研究充実経費

    2016/10 - 2017/03

  13. カテコールアミンと神経疾患に関する研究助成 ノルアドレナリン神経が黒室・線条体ドパミン神経の変性・脱落に及ぼす影響—イムノトキシン神経ターゲティング法による検討 Competitive

    内田 克哉

    Offer Organization: 大日本住友製薬

    System: カテコールアミンと神経疾患に関する研究助成

    2014/04 - 2015/03

  14. ＧＡＢＡインターニューロンの分化・成熟を制御する甲状腺ホルモンの分子基盤の解明 Competitive

    内田 克哉

    Offer Organization: 日本学術振興会

    System: 科学研究補助金基盤研究C

    2012/04 - 2015/03

  15. Regulatory mechanism for processing acute stress responses in the hypothalamus by prolacin-releasing peptide

    ITOI Keiichi, FUSE Toshimitsu, UCHIDA Katsuya, TSUBOKAWA Hiroshi, SAKIMURA Kenji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2011 - 2013

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    Prolactin-releasing peptide (PrRP) is colocalized with noradernaline (NA) in the medulla. We examined whether PrRP and/or NA are involved in activation of neuroendocrine neurons, including corticotropin-releasing factor (CRF) neurons,in the paraventricular nucleus of the hypothalamus (PVN). It was demonstrated that PrRP-containing NA neurons are activated by stress, and that NA and PrRP work in a cooperative manner in the PVN. Using a PVN slice of the CRF-Venus konckin mouse, it was shown that NA stimulates CRF neurons via glutamatergic inputs.

  16. ＧＡＢＡインターニューロン形成を制御する甲状腺ホルモンの新規作用機序の解明 Competitive

    内田 克哉

    Offer Organization: 日本学術振興会

    System: 科学研究補助金基盤研究C

    2010/04 - 2012/03

  17. 動物のスケールフリー行動の一般性とその基本原理の探索 Competitive

    内田 克哉

    Offer Organization: 東北大学学際科学国際高等研究センター

    System: 共同研究助成金

    2009/04 - 2011/03

  18. Introduction of the less invasive diagnosis and treatment technique by using 405nm diode laser into the filed of oral and maxilla of acilal surgery

    MIYAZAKI Hidetaka, UCHIDA Katsuya, KATO Juniji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Gunma University

    2009 - 2011

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    A visible diode laser module which oscillates at the wavelength of 405nm is a newly developed industrial device. We have examined the variety of characteristics of 405 nm laser for aiming the clinical use. The emitted light is efficiently absorbed by porphyrins, which are compounds associated with proteins such as hemoglobin, myoglobin, or melanin pigment, but is not absorbed by water. Due to these absorption characteristics, we observed ex vivo that the 405 nm diode laser could be applied to incision and coagulation of soft tissue at a low power density of irradiation, with little or no carbonization. As the ablation and hemostatic abilitiesof the 405 nm laser should be clarified in vivo, the surgical performance of the 405 nm diode laser was evaluated, using living rat liver tissue. Laser-ablated liver tissue was smooth with observable signs of remnant carbonization and easily acquired hemostasis. The thickness of the denatured layer increased in proportion to the output power ; the coagulation layer did not thicken accordingly. Bleeding could not be stopped for tissues incised with the stainless scalpel. The 405 nm diode laser thus proved to be effective for ablating soft tissue with high hemostatic ability at low power. In the oral and maxillofacial surgery field, an approach to the inferior orbital rim or the orbital floor is a vital procedure for removing orbital tumours. Several techniques have been devised to obtain a large surgical field while avoiding complications such as scar formation and lower eyelid deformity. The swinging eyelid procedure completely avoids the above matters. However, it is not a well-known procedure in other fields, except in the ophthalmology field. We focused on the procedure and introduced it in the field of oral and maxillofacial surgery. We tried to apply the classification of International Society the classification of Vascular Anomlies to oral vascular anomalies, and suggested that in the treatment of vascular lesion, the wavelength characteristics of each type of laser need to be considered for each clinical the type of laser should be carefully selected based on its characteristics of vascular anomalies. We are now studying the wavelength characteristics of 405 nm laser

  19. 成体海馬神経細胞の新生に対するプロスタグランジンE2の作用機序 Competitive

    内田 克哉

    Offer Organization: 日本科学協会

    System: 笹川科学研究助成金

    2003/04 - 2004/03

  20. 歯状回における神経細胞の新生に対するプロスタグランジンの作用 Competitive

    内田 克哉

    Offer Organization: NARISHIGE

    System: 公益信託 成茂神経科学研究助成基金

    2002/04 - 2003/03

  21. 黒色素胞刺激ホルモンの発熱抑制機構—インターロイキンとグルココルチコイドの関与について—1 Competitive

    内田 克哉

    Offer Organization: 日本科学協会

    System: 笹川科学研究助成金

    1998/04 - 1999/03

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Teaching Experience 7

  1. systems bioinfomatics　Tohoku University

  2. 解剖生理学　東京都板橋看護専門学校

  3. 基礎医学修練　東北大学

  4. 創造工学研修　東北大学

  5. 情報倫理学　東北大学

  6. ゲノム情報学　東北大学

  7. 情報生物学　東北大学

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Social Activities 14

  1. 仙台市立第二中学校 二中会 本部役員

    2021/04/01 - Present

  2. 公益財団法人 開高健記念会

    2016/04/01 - Present

  3. 仙台市立立町小学校PTA副会長

    2020/04/10 - 2023/03/31

  4. 仙台市立立町小学校学校関係者評価委員

    2020/04/01 - 2022/03/31

  5. 仙台市立立町小学校PTA広報委員長

    2018/04/01 - 2019/03/31

  6. 仙台市立立町小学校PTA学年委員

    2016/04/01 - 2017/03/31

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    学年行事の企画立案

  7. 仙台市立立町小学校PTA広報委員

    2015/04/01 - 2016/03/31

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    年間行事の編集発行

  8. 東北大学川内けやき保育園保護者会役員会 幹事

    2013/05/11 - 2014/03/31

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    川内けやき保育園保護者会役員会において会計業務を担当

  9. 東北大学川内けやき保育園保護者会役員会 会長

    2012/06 - 2013/05/10

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    保護者会会長

  10. 東北大学川内けやき保育園保護者会役員会 副会長

    2011/04/01 - 2012/06

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    川内けやき保育園 保護者会副会長

  11. 第５回東北シニアビジネス協議会設立準備プレセミナー「脳と心の繋がり〜その時、認知症患者の脳で何が起きているのか？〜」

    2019/11/19 -

  12. 一票に託す

    2017/10/19 -

  13. 東北大学の研究について

    2007/06 -

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    富山第一高等学校にて講演

  14. 脳研究の現在

    2004/06 -

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    埼玉県立上尾原市中学校にて卒業生として「脳研究の現在」と題して講演

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Media Coverage 1

  1. 一票に託す

    毎日新聞　毎日新聞

    2017/10/19

    Type: Newspaper, magazine