Details of the Researcher

PHOTO

Yasuhiro Fukuda
Section
Graduate School of Agricultural Science
Job title
Assistant Professor
Degree

  • 理学博士 (Kanazawa University)

Education 3

  • Kanazawa University　Graduate School, Division of National Science and Technology　Division of Life science

    2006/04 - 2008/03

  • 金沢大学大学院　自然科学研究科　生物科学専攻 博士課程前期

    2004/04 - 2006/03

  • Kanazawa University　Faculty of Science　Department of Biology

    2000/03 - 2004/04

Committee Memberships 14

  • 日本原生生物学会　庶務補佐

    2024/11 - Present

  • Journal of Protistology　Editor in Chief

    2024/11 - Present

  • 日本原生生物学会　評議員

    2021/11 - Present

  • 日本畜産環境学会　編集副委員長

    2021/06 - Present

  • 日本原生生物学会　庶務

    2021/11 - 2024/11

  • International Society of Protistologists (ISOP)　Nominating Committee

    2023/08 - 2024/07

  • 第 4 回 ACOP　プログラム編集委員長

    2021/02 - 2021/11

  • 評議員

    2012/04 - 2018/11

  • 日本藻類学会　第 42 回仙台大会実行委員会

    2018/01 - 2018/03

  • 原生生物学会活性化委員会　委員長

    2013/11 - 2015/11

  • 日本原生生物学会　編集委員会 副編集長

    2010/11 - 2015/11

  • 原生生物学会仙台大会実行委員会　実行委員

    2013/11 - 2014/11

  • 日本原生動物学会 若手の会　役員

    2008/11 - 2013/11

  • The 18th meeting of International Society Of Evolutionary Protistology　Organizing Comitee

    2010/07 -

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Professional Memberships 6

  • 日本分子生物学会

    2014/06 - Present

  • 日本藻類学会

  • International Society of Evolutional Protistology

  • International society of protistologists

  • 日本進化原生生物研究会

  • Japan Society of Protistology

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Research Areas 2

  • Life sciences / Cell biology /

  • Life sciences / Evolutionary biology /

Awards 9

  1. 第39回日本分子生物学会年会優秀ポスター賞

    2016/12/01　日本分子生物学会

  2. 日本畜産環境学会奨励賞

    2015/06/20　日本畜産環境学会

  3. 日本原生動物学会ベストプレゼンテーション賞

    2013/11/10　日本原生動物学会

  4. 持続可能な農林水産業構築プロジェクト 個人型海外研究派遣

    2012/04　東北大学

  5. 日本原生動物学会 奨励賞

    2009/06/01　日本原生動物学会

  6. 日本原生動物学会国際交流助成

    2009/05/15　日本原生動物学会

  7. 第 20 回 加藤記念国際交流助成

    2009　加藤記念バイオサイエンス振興財団

  8. International Society of Evolutionary Protistology Travel Award

    2008/07/26　International Society of Evolutionary Protistology

  9. 第 19 回 加藤財団記念国際交流助成

    2008/05　加藤記念バイオサイエンス振興財団

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Papers 84

  1. Novel ultrastructural features of the nucleus of the ancestral dinoflagellate Oxyrrhis marina as revealed by freeze substitution fixation and volume electron microscopy Peer-reviewed

    Yasuhiro Fukuda, Toshinobu Suzaki, Kazuyoshi Murata, Chihong Song

    Frontiers in Protistology　3　2025/02

    DOI： 10.3389/frpro.2025.1512258 　

  2. Close cooperation between Semi1 and Semi2 proteins is essential for pronuclear positioning in Tetrahymena thermophila Peer-reviewed

    Takahiko Akematsu, Josef Loidl, Yasuhiro Fukuda, Masaaki Iwamoto

    Molecular Biology of the Cell　2025/01/09

    Publisher: American Society for Cell Biology (ASCB)

    DOI： 10.1091/mbc.e24-11-0503 　

    ISSN： 1059-1524

    eISSN： 1939-4586

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    During sexual reproduction in the ciliate Tetrahymena thermophila, meiosis occurs in the germline micronucleus, resulting in the formation of four haploid micronuclei. Of these, only one is selected to evade autophagy, and subsequently migrates to the membrane junction with the partner cell for reciprocal pronuclear exchange. We previously demonstrated that the transmembrane protein Semi1 is essential for this nuclear migration. Semi1 is specifically expressed in mating cells and localizes to the periphery of the selected nucleus. Loss of Semi1 disrupts nuclear attachment to the junction, leading to infertility. However, the mechanism by which Semi1 positions the nucleus at the junction remains unclear. Here, we report that the Semi1-interacting protein, Semi2, is also necessary for proper nuclear positioning. Deletion of Semi2 results in the same nuclear mislocalization phenotype and infertility observed in Semi1 mutant cells. Semi2 colocalizes with Semi1, but in the absence of Semi1, Semi2 fails to exhibit perinuclear localization. The selected nucleus anchors to microtubules prior to migration, a process dependent on both Semi1 and Semi2. We propose a model in which Semi1 recruits Semi2 to the selected nucleus, facilitating the interaction between the nucleus and microtubules required for proper nuclear positioning at the membrane junction.

  3. Molecular characterization and zoonotic risk assessment of Cryptosporidium spp. in Philippine bats Peer-reviewed

    Lin Xu, Yasuhiro Fukuda, Fumi Murakoshi, Phillip Alviola, Joseph Masangkay, Frances Cagayat Recuenco, Ayman Shehata, Tsutomu Omatsu, Hironori Bando, Hikaru Fujii, Yumi Une, Kentaro Kato

    Food and Waterborne Parasitology　e00249-e00249　2024/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.fawpar.2024.e00249 　

    ISSN： 2405-6766

  4. Discovery of a Microbial Carrier with High Adsorption Affinity for Syntrophic Long-Chain Fatty Acid-Degrading Microorganisms. International-journal Peer-reviewed

    Riku Sakurai, Yoshimi Yokoyama, Yasuhiro Fukuda, Masaki Kawakami, Satoshi Hashimoto, Chika Tada

    ACS omega　9　(38)　39643-39651　2024/09/24

    DOI： 10.1021/acsomega.4c03998 　

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    Long-chain fatty acid (LCFA) degradation primarily involves several species of Syntrophomonas and hydrogenotrophic methanogens, constituting the rate-limiting step in anaerobic digestion. It is crucial to augment their abundance to enhance LCFA degradation. Utilizing microbial carriers presents an effective strategy to maintain the microorganisms on the surface and prevent their washout from the digester. In this study, we aimed to identify a suitable microbial carrier with a superior adsorption capacity for LCFA-degrading microorganisms. We tested various polymers, poly(vinyl alcohol) (PVA), polypropylene (PP), polyethylene glycol (PEG), and polyvinylidene chloride (PVDC), adding them to the sludge at the concentration of 28.25 g L-1 and incubating with olive oil. The amplicon sequencing analysis revealed that PVDC retained Syntrophomonas more abundantly than the other polymers. Remarkably, PVDC predominantly adsorbed LCFA-degrading S. sapovorans and S. zehnderi, whereas medium- to short-chain fatty acid-degrading S. wolfei was abundant in the sludge. Moreover, hydrogenotrophic Methanospirillum hungatei was detected at 2.3-9.5 times higher abundance on PVDC compared to the sludge. Further analysis indicated that not only these LCFA-degrading syntrophic microbial communities but also Propionispira and Anaerosinus, which are capable of lipid hydrolysis and glycerol degradation, became dominant on PVDC. Actually, chemical analysis confirmed that adding PVDC promoted the olive oil degradation. These results underscore the potential of PVDC in promoting anaerobic LCFA degradation.

  5. Computational capability of ecological dynamics Peer-reviewed

    Masayuki Ushio, Kazufumi Watanabe, Yasuhiro Fukuda, Yuji Tokudome, Kohei Nakajima

    Royal Society Open Science　10　(4)　2023/04/19

    Publisher: The Royal Society

    DOI： 10.1098/rsos.221614 　

    eISSN： 2054-5703

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    Ecological dynamics is driven by complex ecological networks. Computational capabilities of artificial networks have been exploited for machine learning purposes, yet whether an ecological network possesses a computational capability and whether/how we can use it remain unclear. Here, we developed two new computational/empirical frameworks based on reservoir computing and show that ecological dynamics can be used as a computational resource. In silico ecological reservoir computing (ERC) reconstructs ecological dynamics from empirical time series and uses simulated system responses for information processing, which can predict near future of chaotic dynamics and emulate nonlinear dynamics. The real-time ERC uses real population dynamics of a unicellular organism, Tetrahymena thermophila . The temperature of the medium is an input signal and population dynamics is used as a computational resource. Intriguingly, the real-time ecological reservoir has necessary conditions for computing (e.g. synchronized dynamics in response to the same input sequences) and can make near-future predictions of empirical time series, showing the first empirical evidence that population-level phenomenon is capable of real-time computations. Our finding that ecological dynamics possess computational capability poses new research questions for computational science and ecology: how can we efficiently use it and how is it actually used, evolved and maintained in an ecosystem?

  6. Snf2 Proteins Are Required to Generate Gamete Pronuclei in Tetrahymena thermophila International-journal Peer-reviewed

    Yasuhiro Fukuda, Takahiko Akematsu, Hironori Bando, Kentaro Kato

    Microorganisms　10　(12)　2426-2426　2022/12/07

    Publisher: MDPI AG

    DOI： 10.3390/microorganisms10122426 　

    eISSN： 2076-2607

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    During sexual reproduction/conjugation of the ciliate Tetrahymena thermophila, the germinal micronucleus undergoes meiosis resulting in four haploid micronuclei (hMICs). All hMICs undergo post-meiotic DNA double-strand break (PM-DSB) formation, cleaving their genome. DNA lesions are subsequently repaired in only one ‘selected’ hMIC, which eventually produces gametic pronuclei. DNA repair in the selected hMIC involves chromatin remodeling by switching from the heterochromatic to the euchromatic state of its genome. Here, we demonstrate that, among the 15 Tetrahymena Snf2 family proteins, a core of the ATP-dependent chromatin remodeling complex in Tetrahymena, the germline nucleus specific Iswi in Tetrahymena IswiGTt and Rad5Tt is crucial for the generation of gametic pronuclei. In either gene knockout, the selected hMIC which shows euchromatin markers such as lysine-acetylated histone H3 does not appear, but all hMICs in which markers for DNA lesions persist are degraded, indicating that both IswiGTt and Rad5Tt have important roles in repairing PM-DSB DNA lesions and remodeling chromatin for the euchromatic state in the selected hMIC.

  7. Identification of the evolutionarily conserved nuclear envelope proteins Lem2 and MicLem2 in Tetrahymena thermophila Peer-reviewed

    Masaaki Iwamoto, Yasuhiro Fukuda, Hiroko sakada, Chie Mori, Yasushi Hiraoka, Tokuko Haraguchi

    Gene: X　1　100006　2019/02

    DOI： 10.1016/j.gene.2019.100006 　

  8. Pretreatment with rumen fluid improves methane production in the anaerobic digestion of paper sludge Peer-reviewed

    Shuhei Takizawa, Yasunori Baba, Chika Tada, Yasuhiro Fukuda, Yutaka Nakai

    Waste Management　78　379-384　2018/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.wasman.2018.05.046 　

    ISSN： 0956-053X

  9. Detection and molecular status of Isospora sp from the domestic pigeon (Columba livia domestica) Peer-reviewed

    Ryuma Matsubara, Yasuhiro Fukuda, Fumi Murakoshi, Osamu Nomura, Toru Suzuki, Chika Tada, Yutaka Nakai

    PARASITOLOGY INTERNATIONAL　66　(5)　588-592　2017/10

    DOI： 10.1016/j.parint.2017.05.004 　

    ISSN： 1383-5769

  10. Post-meiotic DNA double-strand breaks occur in Tetrahymena, and require Topoisomerase II and Spo11 Peer-reviewed

    Takahiko Akematsu, Yasuhiro Fukuda, Jyoti Garg, Jeffrey S. Fillingham, Ronald E. Pearlman, Josef Loidl

    ELIFE　6　2017/06

    DOI： 10.7554/eLife.26176 　

    ISSN： 2050-084X

  11. Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena Peer-reviewed

    Masaaki Iwamoto, Hiroko Osakada, Chie Mori, Yasuhiro Fukuda, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi

    JOURNAL OF CELL SCIENCE　130　(10)　1822-1834　2017/05

    DOI： 10.1242/jcs.199398 　

    ISSN： 0021-9533

    eISSN： 1477-9137

  12. Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam Peer-reviewed

    Sam Thi Nguyen, Yasuhiro Fukuda, Duc Tan Nguyen, Chika Tada, Yutaka Nakai

    TROPICAL ANIMAL HEALTH AND PRODUCTION　48　(4)　837-841　2016/04

    DOI： 10.1007/s11250-016-1013-x 　

    ISSN： 0049-4747

    eISSN： 1573-7438

  13. Role of the Cytosolic Heat Shock Protein 70 Ssa5 in the Ciliate Protozoan Tetrahymena thermophila Peer-reviewed

    Yasuhiro Fukuda, Takahiko Akematsu, Rizwan Attiq, Chika Tada, Yutaka Nakai, Ronald E. Pearlman

    JOURNAL OF EUKARYOTIC MICROBIOLOGY　62　(4)　481-493　2015/07

    DOI： 10.1111/jeu.12203 　

    ISSN： 1066-5234

    eISSN： 1550-7408

  14. Role of class III phosphatidylinositol 3-kinase during programmed nuclear death of Tetrahymena thermophila Peer-reviewed

    Takahiko Akematsu, Yasuhiro Fukuda, Rizwan Attiq, Ronald E. Pearlman

    AUTOPHAGY　10　(2)　209-225　2014/02

    DOI： 10.4161/auto.26929 　

    ISSN： 1554-8627

    eISSN： 1554-8635

  15. Molecular characterization of Cryptosporidium in pigs in central Vietnam. International-journal Peer-reviewed

    Sam Thi Nguyen, Yasuhiro Fukuda, Chika Tada, Rintaro Sato, Vu Vy Huynh, Duc Tan Nguyen, Yutaka Nakai

    Parasitology research　112　(1)　187-92　2013/01

    DOI： 10.1007/s00436-012-3124-2 　

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    Little information is available on the epidemiology of Cryptosporidium in pigs in central Vietnam. The aims of this study were to investigate the prevalence and to characterize the genotype distribution of Cryptosporidium isolates in pigs in this region. A total of 193 pig fecal samples were screened for the presence of Cryptosporidium oocysts using the modified Ziehl-Neelsen staining method, and 28 (overall prevalence 14.5 %) were identified as positive by microscopic observation. Positive samples were further analyzed by polymerase chain reaction amplification and sequencing. Genetic identification based on the 18S ribosomal RNA and 70 kDa heat shock protein genes revealed that pigs in Vietnam are infected with two species/genotypes (Cryptosporidium suis and Cryptosporidium pig genotype II). This study is the first molecular characterization of Cryptosporidium in pigs in Vietnam. The presence of these host-adapted species/genotypes suggests that pigs may not pose a significant public health risk in this area. More extensive studies are necessary to ascertain the zoonotic potential of Cryptosporidium in porcine hosts in Vietnam.

  16. Molecular characterization of Cryptosporidium in native beef calves in central Vietnam Peer-reviewed

    Sam Thi Nguyen, Yasuhiro Fukuda, Chika Tada, Rintaro Sato, Binh Duong, Duc Tan Nguyen, Yutaka Nakai

    PARASITOLOGY RESEARCH　111　(4)　1817-1820　2012/10

    DOI： 10.1007/s00436-012-3038-z 　

    ISSN： 0932-0113

    eISSN： 1432-1955

  17. Multilocus genetic analysis of Cryptosporidium parvum from Egypt Peer-reviewed

    Said Amer, Masoud Fayed, Hajime Honma, Yasuhiro Fukuda, Chica Tada, Yutaka Nakai

    PARASITOLOGY RESEARCH　107　(5)　1043-1047　2010/10

    DOI： 10.1007/s00436-010-1967-y 　

    ISSN： 0932-0113

  18. Phylogenetic analyses of the dinoflagellate Noctiluca scintillans based on beta-tubulin and Hsp90 genes Peer-reviewed

    Yasuhiro Fukuda, Hiroshi Endoh

    EUROPEAN JOURNAL OF PROTISTOLOGY　44　(1)　27-33　2008/02

    DOI： 10.1016/j.ejop.2007.07.001 　

    ISSN： 0932-4739

    eISSN： 1618-0429

  19. New details from the complete life cycle of the red-tide dinoflagellate Noctiluca scintillans (Ehrenberg) McCartney. Peer-reviewed

    Fukuda, Yasuhiro Endoh, Hiroshi

    Eur J Protistol　42　(3)　209-219　2006/09

    DOI： 10.1016/j.ejop.2006.05.003 　

  20. An eDNA survey of soil eukaryotic diversity on Minamitorishima Island (Marcus Island) at the easternmost point of Japan Peer-reviewed

    Yasuhiro Fukuda, Kozue Sawada, Satoshi Shimano

    Journal of Protistology　57　2026/05/28

    DOI： 10.18980/jop.e009 　

  21. Simulated Microgravity Causes Faster Replication During Acute Infection of <scp>ME49</scp> ‐ Toxoplasma Gondii and Alterations in Host Cells Peer-reviewed

    Musfika Tasnim Shirin, Takafumi Yamada, Iqra Zafar, Daisuke Kondoh, Hiroki Bochimoto, Hironori Bando, Oluyomi Stephen Adeyemi, Yasuhiro Fukuda, Kentaro Kato

    FASEB BioAdvances　8　(4)　2026/03/31

    Publisher: Wiley

    DOI： 10.1096/fba.2025-00287 　

    ISSN： 2573-9832

    eISSN： 2573-9832

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    ABSTRACT Spaceflight is important to explore the universe. However, astronauts face many health challenges in space including the increased risk of infection. During spaceflight, the microgravity weakens immunity, which causes opportunistic infections with pathogens such as herpesvirus. However, we have no information about the effects of microgravity on infections caused by other pathogens with a latent stage in their life cycle. In addition, there are no reports on host cell responses or the mechanisms that modulate changes under microgravity conditions. Due to the high cost and effort required for space study in actual microgravity, simulated microgravity plays an important role in mimicking conditions that allow researchers to study risks during spaceflights. Toxoplasma gondii is one of the top five important economic pathogens; it causes toxoplasmosis, may reactivate from the latent phase in immunocompromised individuals, and there is no active drug or vaccine. In this study, we observed the effect of simulated microgravity on T. gondii and on host cells. Simulated microgravity using a 3D clinostat induced host cell proliferation and caused aggregated cells to form three dimensional multicellular spheroids in Vero cells and HFF. Rapid egress and growth of tachyzoites during the acute phase of type II ME49 infection were observed under simulated microgravity.

  22. Identification of Cryptosporidium and Eimeria associated with diarrhea in calves in Japan (2020–2022) Peer-reviewed

    Mohammad Hazzaz Bin Kabir, Fumi Murakoshi, Yasuhiro Fukuda, Megumi Itoh, Yuto Kano, Reiichiro Sato, Konosuke Otomaru, Atsushi Fujikura, Takuto Endo, Fuminosuke Yamamura, Akira Goto, Kenichi Shibano, Nanako Mochizuki, Fumiaki Takahashi, Yosuke Maeda, Hiroshi Miura, Kazuhiro Bandai, Kentaro Kato

    Parasitology Research　125　(1)　2026/02/10

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00436-026-08638-w 　

    ISSN： 0932-0113

    eISSN： 1432-1955

  23. Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei. International-journal Peer-reviewed

    Mamoru Niikura, Yuichi Koyama, Yasuhiro Fukuda, Kentaro Kato, Toshiyuki Fukutomi

    Malaria journal　25　(1)　108-108　2026/02/03

    DOI： 10.1186/s12936-026-05786-3 　

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    BACKGROUND: Serine/arginine-rich splicing factor 1 (SR1), an orthologue of hypothetical RNA-binding protein (HRB1) in yeast, is essential to the asexual development of Plasmodium falciparum and P. berghei. However, their interacting proteins in malaria parasites remain unclear. METHODS: To identify SR1-interacting proteins in malaria parasites, transgenic Pb ANKA expressing green fluorescent protein-fused PbSR1 (PBANKA_1232100) was generated and performed immunoprecipitation coupled to mass spectrometry (IP-MS) using the transgenic parasites. To investigate the developmental stage at which PbSR1 and RhopH2 are expressed, transgenic parasites co-expressing the fusion proteins PbSR1::GFP and RhopH2::mCherry were generated and western blot analysis and live-cell fluorescence imaging were performed. RESULTS: The fluorescence signal of PbSR1::GFP was stronger in nuclei than the cytoplasm of malaria parasites. IP-MS of the transgenic parasites indicated that PbSR1 interacts with nuclear proteins, including RNA-binding protein and small nuclear ribonucleoprotein, and cytoplasmic proteins, such as RhopH1 (or Clag3), RhopH2, and RhopH3. Live-cell fluorescence imaging showed that co-localization of PbSR1 and RhopH2 in the cytoplasm was observed in trophozoites and gametocytes but not mature schizonts and merozoites. From these results, trophozoites, immature schizonts and gametocytes are candidate stages at which cytoplasmic PbSR1 interacts with RhopH2. CONCLUSIONS: PbSR1 interacts with nuclear proteins and rhoptry proteins.

  24. Culture-independent discovery of a novel thermotolerant lipase and its producer from mesophilic anaerobic digestion sludge Peer-reviewed

    Riku Sakurai, Yasuhiro Fukuda, Chika Tada

    Applied Microbiology and Biotechnology　109　(1)　2025/12/24

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00253-025-13674-0 　

    eISSN： 1432-0614

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    Abstract Anaerobic digestion of lipid-rich wastes holds significant potential for enhanced biomethane production, due to the high energy density of lipids. However, to fully harness this potential, a deeper understanding of lipolytic microorganisms is essential, as key microbial players involved in lipid hydrolysis remain largely unidentified. In this study, we employed an integrated approach combining zymography, metaproteomics, and metagenomics to identify the lipolytic microorganisms from anaerobic digester sludge. This activity-based strategy identified a novel lipase distantly related to known lipases. Besides, although this lipase originates from a mesophilic environment, it exhibited unexpected extremophilic-like properties, with maximal activity at 97.5 °C and pH 11. We further reconstructed a metagenome-assembled genome encoding this lipase and demonstrated that it likely represents a novel genus closely related to Candidatus Scatomorpha. Metabolic reconstruction suggested that this bacterium hydrolyzes extracellular lipids and utilizes the resulting hydrolysate, glycerol, to produce lactate and ethanol. Habitat analysis revealed that this bacterium is specifically detected in anaerobic digesters, particularly those processing lipid-rich waste. These findings highlight the pivotal role of this bacterium in anaerobic lipid degradation.

  25. Artifact-free preparation of biological samples for SEM by optimized water freeze-drying Peer-reviewed

    Hideki Ishida, Naoya Fukuda, Maho Shimada, Kousei Yamamoto, Liudmyla Gaponova, Rina Higuchi, Andrii Kolosiuk, Ryo Hoshina, Mikihiko Arikawa, Yasuhiro Fukuda, MD Shafiq Islam, Terue Harumoto, Yumeng Wan, Toshiki Itoh, Yoko Inai, Asuka Takeishi, Hitoshi Aonuma, Takuto Kikuchi, Shijo Nishigori, Tadashi Maruyama, Kenichi Ikeda, Hideyuki Iriko, Toshinobu Suzaki

    Scientific Reports　2025/12/10

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-025-30335-4 　

    eISSN： 2045-2322

  26. Circular metagenome-assembled genome of Candidatus Cloacimonadota recovered from anaerobic digestion sludge. International-journal Peer-reviewed

    Riku Sakurai, Yasuhiro Fukuda, Chika Tada

    Microbiology resource announcements　13　(7)　e0040324　2024/07/18

    DOI： 10.1128/mra.00403-24 　

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    This study reports a circular metagenome-assembled genome (cMAG) of Candidatus Cloacimonadota recovered from a mesophilic full-scale food waste treatment plant. The cMAG spans 2,298,113 bp, with 980× coverage and 1 contig.

  27. Circular metagenome-assembled genome of Candidatus Patescibacteria recovered from anaerobic digestion sludge. International-journal Peer-reviewed

    Riku Sakurai, Yasuhiro Fukuda, Chika Tada

    Microbiology resource announcements　13　(4)　e0008324　2024/04/11

    DOI： 10.1128/mra.00083-24 　

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    A single-contig, circular metagenome-assembled genome (cMAG) of Candidatus (Ca.) Patescibacteria was reconstructed from a mesophilic full-scale food waste treatment plant in Japan. The genome is of small size and lacks fundamental biosynthetic pathways. Taxonomic analysis using the Genome Taxonomy Database revealed that this cMAG belonged to the genus JAEZRQ01 (Ca. Parcubacteria).

  28. Goethite and Hematite Nanoparticles Show Promising Anti-Toxoplasma Properties. International-journal Peer-reviewed

    Kosei Ishii, Eiji Akahoshi, Oluyomi Stephen Adeyemi, Hironori Bando, Yasuhiro Fukuda, Tomoyuki Ogawa, Kentaro Kato

    Pharmaceutics　16　(3)　2024/03/18

    DOI： 10.3390/pharmaceutics16030413 　

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    Toxoplasma gondii is an intracellular parasitic protozoan with a high infection rate in mammals, including humans, and birds. There is no effective vaccine, and treatment relies on antiparasitic drugs. However, existing antiprotozoal drugs have strong side effects and other problems; therefore, new treatment approaches are needed. Metal nanoparticles have attracted increased interest in the biomedical community in recent years because of their extremely high surface area to volume ratio and their unique reactivity that could be exploited for medicinal purposes. Previously, we confirmed the anti-Toxoplasma effects of gold, silver, and platinum nanoparticles, in a growth inhibition test. Here, we asked whether the anti-Toxoplasma effect could be confirmed with less expensive metal nanoparticles, specifically iron oxide nanoparticles (goethite and hematite). To improve the selective action of the nanoparticles, we modified the surface with l-tryptophan as our previous findings showed that the bio-modification of nanoparticles enhances their selectivity against T. gondii. Fourier-Transform Infrared Spectroscopy (FTIR) analysis confirmed the successful coating of the iron oxide nanoparticles with l-tryptophan. Subsequently, cytotoxicity and growth inhibition assays were performed. L-tryptophan-modified nanoparticles showed superior anti-Toxoplasma action compared to their naked nanoparticle counterparts. L-tryptophan enhanced the selective toxicity of the iron oxide nanoparticles toward T. gondii. The bio-modified nanoparticles did not exhibit detectable host cell toxicity in the effective anti-Toxoplasma doses. To elucidate whether reactive oxygen species contribute to the anti-Toxoplasma action of the bio-modified nanoparticles, we added Trolox antioxidant to the assay medium and found that Trolox appreciably reduced the nanoparticle-induced growth inhibition.

  29. Author's correction: Relationship Between Rumen Microbial Composition and Fibrolytic Isozyme Activity During the Biodegradation of Rice Straw Powder Using Rumen Fluid.

    Shuhei Takizawa, Ryoki Asano, Kenichi Abe, Yasuhiro Fukuda, Yasunori Baba, Riku Sakurai, Chika Tada, Yutaka Nakai

    Microbes and environments　39　(2)　2024

    DOI： 10.1264/jsme2.ME23041e 　

  30. Toxoplasma gondii chitinase-like protein TgCLP1 regulates the parasite cyst burden. International-journal Peer-reviewed

    Hironori Bando, Yuho Murata, Yongmei Han, Tatsuki Sugi, Yasuhiro Fukuda, David J Bzik, Barbara A Fox, Kentaro Kato

    Frontiers in cellular and infection microbiology　14　1359888-1359888　2024

    DOI： 10.3389/fcimb.2024.1359888 　

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    Toxoplasma, an important intracellular parasite of humans and animals, causes life-threatening toxoplasmosis in immunocompromised individuals. Although Toxoplasma secretory proteins during acute infection (tachyzoite, which divides rapidly and causes inflammation) have been extensively characterized, those involved in chronic infection (bradyzoite, which divides slowly and is surrounded by a cyst wall) remain uncertain. Regulation of the cyst wall is essential to the parasite life cycle, and polysaccharides, such as chitin, in the cyst wall are necessary to sustain latent infection. Toxoplasma secretory proteins during the bradyzoite stage may have important roles in regulating the cyst wall via polysaccharides. Here, we focused on characterizing the hypothetical T. gondii chitinase, chitinase-like protein 1 (TgCLP1). We found that the chitinase-like domain containing TgCLP1 is partially present in the bradyzoite microneme and confirmed, albeit partially, its previous identification in the tachyzoite microneme. Furthermore, although parasites lacking TgCLP1 could convert from tachyzoites to bradyzoites and make an intact cyst wall, they failed to convert from bradyzoites to tachyzoites, indicating that TgCLP1 is necessary for bradyzoite reactivation. Taken together, our findings deepen our understanding of the molecular basis of recrudescence and could contribute to the development of novel strategies for the control of toxoplasmosis.

  31. Antiplasmodial and interferon-gamma-modulating activities of the aqueous extract of stone breaker (Phyllanthus niruri Linn.) in malaria infection. International-journal Peer-reviewed

    Temitope Olawale Jeje, Hironori Bando, Md Thoufic Anam Azad, Yasuhiro Fukuda, Ibukun Emmanuel Oluwafemi, Kentaro Kato

    Parasitology international　97　102789-102789　2023/07/18

    DOI： 10.1016/j.parint.2023.102789 　

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    Plasmodium falciparum parasites are the primary cause of malaria across Africa. The problem of drug resistance to malaria is ever growing and novel therapeutic strategies need to be developed, particularly those targeting the parasite and also the host or host-pathogen interaction. Previous studies have shown that the development of cerebral malaria (CM) is related to dysregulation of the immune system in a murine malaria model of experimental cerebral malaria. It involves a complex interaction of events and interferon-gamma seems to be the unifying factor. Therefore, the antiplasmodial activity targeting the parasite and immunomodulatory strategies that reduce overall host inflammation, with IFN-γ in focus, could delay CM onset and prove beneficial in malaria infection therapy. Phyllanthus niruri is used to treat fever and other symptoms of malaria in Nigeria. Its modes of action as an anti-malarial remedy have not been exhaustively investigated. This study therefore examined the aqueous extract of P. niruri (PE) for its antiplasmodial activity in vitro using the Plasmodium falciparum HB3 strain. Furthermore, in vivo murine malaria model using the Plasmodium berghei ANKA strain was used to investigate its anti-malarial effects. We showed that PE has multiple anti-malarial effects, including anti-parasitic and host immunomodulatory activities. Co-culture of P. falciparum with PE and some of its phytoconstituents drastically reduced parasite number. PE also decreased parasitemia, and increased the survival of infected mice. We also observed that the integrity of the blood-brain barrier was maintained in the PE-treated mice. The results confirmed that PE showed moderate antiplasmodial activity. In vivo murine malaria model using P. berghei ANKA for experimental cerebral malaria revealed that PE suppressed parasite growth, and modulate the production of interferon-gamma. The findings demonstrate that PE affects malaria progression, targeting parasites and host cells.

  32. Species-specific Primer and Probe Sets for Detection of Syntrophic Long-chain Fatty Acid-degrading Bacteria in Anaerobic Digestion Using Quantitative PCR Peer-reviewed

    Riku Sakurai, Yasuhiro Fukuda, Chika Tada

    Microbes and Environments　38　(2)　n/a-n/a　2023/06/24

    Publisher: Japanese Society of Microbial Ecology

    DOI： 10.1264/jsme2.me23023 　

    ISSN： 1342-6311

    eISSN： 1347-4405

  33. The role of atypical MAP kinase 4 in the host interaction with Cryptosporidium parvum. International-journal Peer-reviewed

    Nina Watanabe, Hironori Bando, Fumi Murakoshi, Riku Sakurai, Mohammad Hazzaz Bin Kabir, Yasuhiro Fukuda, Kentaro Kato

    Scientific reports　13　(1)　1096-1096　2023/01/19

    DOI： 10.1038/s41598-023-28269-w 　

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    Cryptosporidium parvum is an apicomplexan parasite that causes severe zoonotic diarrhea in humans and calves. Since there are no effective treatments or vaccines for infants or immunocompromised patients, it is important to understand the molecular mechanisms of the parasite-host interaction for novel drug discovery. Mitogen-activated protein kinase (MAP kinase) is a key host factor in interactions between host and various pathogens, including parasites. Although the function of conventional MAP kinases against parasite infection has been investigated, that of atypical MAP kinases remains largely unknown. Therefore, we focused on one of the atypical MAP kinases, MAPK4, and its effect on C. parvum infection in human intestinal cells. Here, we report that MAPK4-deficient intestinal cells showed a significant reduction in C. parvum infection. We also show that host MAPK4 has a role in host cell survival from C. parvum infection. In addition, we show that C. parvum requires host MAPK4 for its successful invasion and asexual reproduction. Taken together, our data suggest that MAPK4 is an important host factor contributing to C. parvum infection in human intestinal cells.

  34. Relationship Between Rumen Microbial Composition and Fibrolytic Isozyme Activity During the Biodegradation of Rice Straw Powder Using Rumen Fluid Peer-reviewed

    Shuhei Takizawa, Ryoki Asano, Kenichi Abe, Yasuhiro Fukuda, Yasunori Baba, Riku Sakurai, Chika Tada, Yutaka Nakai

    Microbes and Environments　38　(3)　2023

    Publisher: Japanese Society of Microbial Ecology

    DOI： 10.1264/jsme2.me23041 　

    ISSN： 1342-6311

    eISSN： 1347-4405

  35. Quantification of free and esterified long-chain fatty acids without extraction by high-performance liquid chromatography in anaerobic digestion sludge Peer-reviewed

    Riku Sakurai, Yoshimi Yokoyama, Yasuhiro Fukuda, Chika Tada

    Journal of Material Cycles and Waste Management　25　(2)　810-815　2022/12/13

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10163-022-01561-z 　

    ISSN： 1438-4957

    eISSN： 1611-8227

  36. Identification of potent anti-Cryptosporidium new drug leads by screening traditional Chinese medicines. International-journal Peer-reviewed

    Mohammad Hazzaz Bin Kabir, Frances Cagayat Recuenco, Nur Khatijah Mohd Zin, Nina Watanabe, Yasuhiro Fukuda, Hironori Bando, Kenichi Watanabe, Hiroki Bochimoto, Xuenan Xuan, Kentaro Kato

    PLoS neglected tropical diseases　16　(11)　e0010947　2022/11

    DOI： 10.1371/journal.pntd.0010947 　

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    Cryptosporidium spp. are gastrointestinal opportunistic protozoan parasites that infect humans, domestic animals, and wild animals all over the world. Cryptosporidiosis is the second leading infectious diarrheal disease in infants less than 5 years old. Cryptosporidiosis is a common zoonotic disease associated with diarrhea in infants and immunocompromised individuals. Consequently, cryptosporidiosis is considered a serious economic, veterinary, and medical concern. The treatment options for cryptosporidiosis are limited. To address this problem, we screened a natural product library containing 87 compounds of Traditional Chinese Medicines for anti-Cryptosporidium compounds that could serve as novel drug leads and therapeutic targets against C. parvum. To examine the anti-Cryptosporidium activity and half-maximal inhibitory doses (EC50) of these compounds, we performed in vitro assays (Cryptosporidium growth inhibition assay and host cell viability assay) and in vivo experiments in mice. In these assays, the C. parvum HNJ-1 strain was used. Four of the 87 compounds (alisol-A, alisol-B, atropine sulfate, and bufotalin) showed strong anti-Cryptosporidium activity in vitro (EC50 values = 122.9±6.7, 79.58±13.8, 253.5±30.3, and 63.43±18.7 nM, respectively), and minimum host cell cytotoxicity (cell survival > 95%). Furthermore, atropine sulfate (200 mg/kg) and bufotalin (0.1 mg/kg) also showed in vivo inhibitory effects. Our findings demonstrate that atropine sulfate and bufotalin are effective against C. parvum infection both in vitro and in vivo. These compounds may, therefore, represent promising novel anti-Cryptosporidium drug leads for future medications against cryptosporidiosis.

  37. Depletion of Intracellular Glutamine Pools Triggers Toxoplasma gondii Stage Conversion in Human Glutamatergic Neurons International-journal Peer-reviewed

    Hironori Bando, Yasuhiro Fukuda, Nina Watanabe, Jeje Temitope Olawale, Kentaro Kato

    Frontiers in Cellular and Infection Microbiology　11　788303-788303　2022/01/13

    Publisher: Frontiers Media SA

    DOI： 10.3389/fcimb.2021.788303 　

    eISSN： 2235-2988

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    <italic>Toxoplasma gondii</italic> chronically infects the brain as latent cysts containing bradyzoites and causes various effects in the host. Recently, the molecular mechanisms of cyst formation in the mouse brain have been elucidated, but those in the human brain remain largely unknown. Here, we show that abnormal glutamine metabolism caused by both interferon-γ (IFN-γ) stimulation and <italic>T. gondii</italic> infection induce cyst formation in human neuroblastoma cells regardless of the anti-<italic>T. gondii</italic> host factor nitric oxide (NO) level or Indoleamine 2,3-dioxygenase-1 (IDO1) expression. IFN-γ stimulation promoted intracellular glutamine degradation in human neuronal cells. Additionally, <italic>T. gondii</italic> infection inhibited the mRNA expression of the host glutamine transporters SLC38A1 and SLC38A2. These dual effects led to glutamine starvation and triggered <italic>T. gondii</italic> stage conversion in human neuronal cells. Furthermore, these mechanisms are conserved in human iPSC-derived glutamatergic neurons. Taken together, our data suggest that glutamine starvation in host cells is an important trigger of <italic>T. gondii</italic> stage conversion in human neurons.

  38. Shifts in xylanases and the microbial community associated with xylan biodegradation during treatment with rumen fluid International-journal Peer-reviewed

    Shuhei Takizawa, Ryoki Asano, Yasuhiro Fukuda, Yasunori Baba, Chika Tada, Yutaka Nakai

    Microbial Biotechnology　2021/12/28

    Publisher: Wiley

    DOI： 10.1111/1751-7915.13988 　

    ISSN： 1751-7915

    eISSN： 1751-7915

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    Treatment with rumen fluid improves methane production from non-degradable lignocellulosic biomass during subsequent methane fermentation; however, the kinetics of xylanases during treatment with rumen fluid remain unclear. This study aimed to identify key xylanases contributing to xylan degradation and their individual activities during xylan treatment with bovine rumen microorganisms. Xylan was treated with bovine rumen fluid at 37°C for 48 h under anaerobic conditions. Total solids were degraded into volatile fatty acids and gases during the first 24 h. Zymography showed that xylanases of 24, 34, 85, 180, and 200 kDa were highly active during the first 24 h. Therefore, these xylanases are considered to be crucial for xylan degradation during treatment with rumen fluid. Metagenomic analysis revealed that the rumen microbial community's structure and metabolic function temporally shifted during xylan biodegradation. Although statistical analyses did not reveal significantly positive correlations between xylanase activities and known xylanolytic bacterial genera, they positively correlated with protozoal (e.g., Entodinium, Diploplastron, and Eudiplodinium) and fungal (e.g., Neocallimastix, Orpinomyces, and Olpidium) genera and unclassified bacteria. Our findings suggest that rumen protozoa, fungi, and unclassified bacteria are associated with key xylanase activities, accelerating xylan biodegradation into volatile fatty acids and gases, during treatment of lignocellulosic biomass with rumen fluid.

  39. Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion Peer-reviewed

    Riku Sakurai, Shuhei Takizawa, Yasuhiro Fukuda, Chika Tada

    PLOS ONE　16　(9)　e0257651-e0257651　2021/09/30

    Publisher: Public Library of Science (PLoS)

    DOI： 10.1371/journal.pone.0257651 　

    eISSN： 1932-6203

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    Scum is formed by the adsorption of long-chain fatty acids (LCFAs) onto biomass surface in anaerobic digestion of oily substrates. Since scum is a recalcitrant substrate to be digested, it is disposed via landfilling or incineration, which results in biomass washout and a decrease in methane yield. The microbes contributing to scum degradation are unclear. This study aimed to investigate the cardinal microorganisms in anaerobic scum digestion. We pre-incubated a sludge with scum to enrich scum-degrading microbes. Using this sludge, a 1.3-times higher methane conversion rate (73%) and a faster LCFA degradation compared with control sludge were attained. Then, we analyzed the cardinal scum-degrading microbes in this pre-incubated sludge by changing the initial scum-loading rates. Increased 16S rRNA copy numbers for the syntrophic fatty-acid degrader <italic>Syntrophomonas</italic> and hydrogenotrophic methanogens were observed in scum high-loaded samples. 16S rRNA amplicon sequencing indicated that <italic>Syntrophomonas</italic> was the most abundant genus in all the samples. The amino-acid degrader <italic>Aminobacterium</italic> and hydrolytic genera such as <italic>Defluviitoga</italic> and <italic>Sporanaerobacter</italic> became more dominant as the scum-loading rate increased. Moreover, phylogenic analysis on <italic>Syntrophomonas</italic> revealed that <italic>Syntrophomonas palmitatica</italic>, which is capable of degrading LCFAs, related species became more dominant as the scum-loading rate increased. These results indicate that a variety of microorganisms that degrade LCFAs, proteins, and sugars are involved in effective scum degradation.

  40. Synergistic oocysticidal effect of Ortho-reagent and saturated calcium hydroxide solution against Cryptosporidium parvum Peer-reviewed

    Ryuma Matsubara, Fumi Murakoshi, Chika Tada, Yasuhiro Fukuda, Yutaka Nakai

    Journal of Animal Production Environment Science　21　(1)　2021/09

  41. Characteristics of various fibrolytic isozyme activities in the rumen microbial communities of Japanese Black and Holstein Friesian cattle under different conditions Peer-reviewed

    Shuhei Takizawa, Ryoki Asano, Yasuhiro Fukuda, Yasunori Baba, Chika Tada, Yutaka Nakai

    Animal Science Journal　92　(1)　2021/01

    Publisher: Wiley

    DOI： 10.1111/asj.13653 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  42. Change of Endoglucanase Activity and Rumen Microbial Community During Biodegradation of Cellulose Using Rumen Microbiota International-journal Peer-reviewed

    Shuhei Takizawa, Ryoki Asano, Yasuhiro Fukuda, Mengjia Feng, Yasunori Baba, Kenichi Abe, Chika Tada, Yutaka Nakai

    Frontiers in Microbiology　11　603818-603818　2020/12/18

    Publisher: Frontiers Media SA

    DOI： 10.3389/fmicb.2020.603818 　

    eISSN： 1664-302X

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    Treatment with rumen microorganisms improves the methane fermentation of undegradable lignocellulosic biomass; however, the role of endoglucanase in lignocellulose digestion remains unclear. This study was conducted to investigate endoglucanases contributing to cellulose degradation during treatment with rumen microorganisms, using carboxymethyl cellulose (CMC) as a substrate. The rate of CMC degradation increased for the first 24 h of treatment. Zymogram analysis revealed that endoglucanases of 52 and 53 kDa exhibited high enzyme activity for the first 12 h, whereas endoglucanases of 42, 50, and 101 kDa exhibited high enzyme activities from 12 to 24 h. This indicates that the activities of these five endoglucanases shifted and contributed to efficient CMC degradation. Metagenomic analysis revealed that the relative abundances of <italic>Selenomonas</italic>, <italic>Eudiplodinium</italic>, and <italic>Metadinium</italic> decreased after 12 h, which was positively correlated with the 52- and 53-kDa endoglucanases. Additionally, the relative abundances of <italic>Porphyromonas</italic>, <italic>Didinium</italic>, unclassified Bacteroidetes, <italic>Clostridiales</italic> family XI, <italic>Lachnospiraceae</italic> and <italic>Sphingobacteriaceae</italic> increased for the first 24 h, which was positively correlated with endoglucanases of 42, 50, and 101 kDa. This study suggests that uncharacterized and non-dominant microorganisms produce and/or contribute to activity of 40, 50, 52, 53, and 101 kDa endoglucanases, enhancing CMC degradation during treatment with rumen microorganisms.

  43. Remarkable Removal of Antibiotic-Resistant Bacteria During Dairy Wastewater Treatment Using Hybrid Full-scale Constructed Wetland Peer-reviewed

    Hosnia S. Abdel-Mohsein, Mengjia Feng, Yasuhiro Fukuda, Chika Tada

    Water, Air, & Soil Pollution　231　(8)　2020/08

    DOI： 10.1007/s11270-020-04775-9 　

    ISSN： 0049-6979

    eISSN： 1573-2932

  44. Development of a highly sensitive method for the detection of cryptosporidium parvum virus type 1 (Cspv1) Peer-reviewed

    Ayman Ahmed Shehata, Hironori Bando, Yasuhiro Fukuda, Mohammad Hazzaz Bin Kabir, Fumi Murakoshi, Megumi Itoh, Atsushi Fujikura, Hiroaki Okawa, Takuto Endo, Akira Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, Konosuke Otomaru, Kei Kazama, Mohamed Ibrahim Essa, Kentaro Kato

    Japanese Journal of Veterinary Research　68　(3)　159-170　2020/08

    DOI： 10.14943/jjvr.68.3.159 　

    ISSN： 0047-1917

  45. Identification of bacteria involved in the decomposition of lignocellulosic biomass treated with cow rumen fluid by metagenomic analysis Peer-reviewed

    Chol Gyu Lee, Yasunori Baba, Ryoki Asano, Yasuhiro Fukuda, Chika Tada, Yutaka Nakai

    Journal of Bioscience and Bioengineering　130　(2)　137-141　2020/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.jbiosc.2020.03.010 　

    ISSN： 1389-1723

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    We had developed a new pretreatment system using cow rumen fluid to improve the methane production from lignocellulosic substrates. However, the pretreatment conditions differ from the in-situ rumen environment, therefore different microbes may be involved in plant cell wall decomposition. In the current study, shotgun metagenomic analysis using MiSeq platform was performed to elucidate the bacteria which produce cellulase and hemicellulase in this pretreatment system. The rumen fluid which contained waste paper pieces (0.1% w/v) were incubated at 37°C during 120 h. The fluid samples were collected from the reactor at each time-point and analyzed for chemical properties. Rumen microbial DNA was extracted from 0-h and 60-h samples and subjected to shotgun-metagenomic analysis. After pretreatment, approximately half of cellulose and hemicellulose contents of the waste paper were decomposed and some volatile fatty acids were accumulated. Clostridia (e.g., Ruminococcus and Clostridium) were the predominant bacteria before and after 60-h pretreatment, and their relative abundance was increased during pretreatment. However, Prevotella and Fibrobacter, one of the most dominant bacteria in-situ rumen fluid, were observed less than 3% before incubation and they were decreased after pretreatment. Genes encoding cellulase and hemicellulase were mainly found in Ruminococcus, Clostridium, and Caldicellulosiruptor. Calicellulosiruptor, which had not been previously identified as the predominant genus in lignocellulose decomposition in in-situ rumen conditions, might be considered as the main fibrolytic bacterium in this system. Thus, this study demonstrated that the composition of fibrolytic bacteria in this system was greatly different from those in the in-situ rumen.

  46. Diversity of Sulfur-oxidizing Bacteria at the Surface of Cattle Manure Composting Assessed by an Analysis of the Sulfur Oxidation Gene soxB Peer-reviewed

    Yumi Mori, Chika Tada, Yasuhiro Fukuda, Yutaka Nakai

    Microbes and Environments　35　(3)　2020/07

    Publisher: Japanese Society of Microbial Ecology

    DOI： 10.1264/jsme2.me18066 　

    ISSN： 1342-6311

    eISSN： 1347-4405

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    Sulfur-oxidizing bacterial diversity at the surface of cattle manure was characterized throughout the composting process using a sulfur oxidation gene (soxB) clone library approach. In the mesophilic phase, clones related to the genera Hydrogenophaga and Hydrogenophilus were characteristically detected. In the thermophilic phase, clones related to the genera Hydrogenophaga and Thiohalobacter were predominant. In the cooling phase, the predominant soxB sequences were related to the genus Pseudaminobacter and a new sulfur-oxidizing bacterium belonging to the class Alphaproteobacteria. The present study showed changes in the community composition of sulfur-oxidizing bacteria at the surface of compost throughout the composting process.

  47. Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan. International-journal Peer-reviewed

    Mohammad Hazzaz Bin Kabir, Megumi Itoh, Ayman Ahmed Shehata, Hironori Bando, Yasuhiro Fukuda, Fumi Murakoshi, Atsushi Fujikura, Hiroaki Okawa, Takuto Endo, Akira Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, Konosuke Otomaru, Mohamed Ibrahim Essa, Kei Kazama, Xuenan Xuan, Kentaro Kato

    Parasitology international　78　102153-102153　2020/06/03

    DOI： 10.1016/j.parint.2020.102153 　

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    Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan.

  48. Recovery of the fibrolytic microorganisms from rumen fluid by flocculation for simultaneous treatment of lignocellulosic biomass and volatile fatty acid production Peer-reviewed

    Shuhei Takizawa, Kenichi Abe, Yasuhiro Fukuda, Mengjia Feng, Yasunori Baba, Chika Tada, Yutaka Nakai

    Journal of Cleaner Production　257　120626-120626　2020/06

    DOI： 10.1016/j.jclepro.2020.120626 　

    ISSN： 0959-6526

    eISSN： 1879-1786

  49. Sodium dodecyl sulfate improves the treatment of waste paper with rumen fluid at lower concentration but decreases at higher condition Peer-reviewed

    Shuhei Takizawa, Yasunori Baba, Chika Tada, Yasuhiro Fukuda, Yutaka Nakai

    Journal of Material Cycles and Waste Management　22　(3)　656-663　2020/05

    DOI： 10.1007/s10163-019-00957-8 　

    ISSN： 1438-4957

    eISSN： 1611-8227

  50. Toxoplasma gondii effectors TgIST and TgGRA15 differentially target host IDO1 to antagonize the IFN-γ-induced anti-T. gondii response in human cells.

    Bando, H, Fukuda, Y, Yamamoto, M, Kato K

    Journal of Integrated Field Science　17　37-37　2020/03

  51. Functional Group Distribution of the Carrier Surface Influences Adhesion of Methanothermobacter thermautotrophicus International-journal Peer-reviewed

    Masaki Umetsu, Takaaki Sunouchi, Yasuhiro Fukuda, Hideyuki Takahashi, Chika Tada

    Archaea　2020　1-8　2020/01/23

    Publisher: Hindawi Limited

    DOI： 10.1155/2020/9432803 　

    ISSN： 1472-3646

    eISSN： 1472-3654

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    Various support carriers are used for high-density retention of methanogenic archaea in anaerobic wastewater treatment systems. Although the physicochemical properties of carrier materials and microorganisms influence the adhesion of methanogenic archaea, details about the underlying mechanism remain poorly characterized. We applied seven types of chemical surface modifications to carbon felts to clarify the adhesion properties of <italic>Methanothermobacter thermautotrophicus</italic>, a representative thermophilic hydrogenotrophic methanogen. The relationship between carrier surface properties and methanogen adhesion was evaluated. <italic>M. thermautotrophicus</italic> adhesion was significantly increased up to 2.6 times in comparison with control on carbon felts treated with NaOH, HCl, H2SO4, or Na2HPO4. Treated carbon felts showed a lower water contact angle, but no correlation between the carrier surface contact angle and methanogen adhesion was observed. On the other hand, at the surface of the carrier that showed improved adhesion of methanogens, the ratio of -COOH : -OH was 1 : 0.65. Such a ratio was not observed with treated carriers for which methanogen adhesion was not improved. Therefore, in the adhesion of <italic>M. thermautotrophicus</italic>, the functional group abundance was important as well as physical surface properties such as the hydrophobicity. Hydrogenotrophic methanogens are involved in active methanation during the startup of anaerobic digestion. Additionally, these methanogenic archaea function as methanogenic cathode catalysts. Therefore, anaerobic digestion performance will greatly improve by controlling the adhesion of hydrogenotrophic methanogens such as <italic>M. thermautotrophicus</italic>.

  52. Electricity Generation by a Methanogen Cathode Microbial Fuel Cell Peer-reviewed

    Masaki UMETSU, Yasuhiro FUKUDA, Hideyuki TAKAHASHI, Chika TADA

    Journal of Animal Production Environment Science　2019/08

  53. Preservation of rumen fluid for the pretreatment of waste paper to improve methane production Peer-reviewed

    Shuhei Takizawa, Yasunori Baba, Chika Tada, Yasuhiro Fukuda, Yutaka Nakai

    Waste Management　87　672-678　2019/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.wasman.2019.02.043 　

    ISSN： 0956-053X

  54. Pretreatment of lignocellulosic biomass with cattle rumen fluid for methane production: Fate of added rumen microbes and indigenous microbes of methane seed sludge Peer-reviewed

    Yasunori Baba, Yu Matsuki, Shuhei Takizawa, Yoshihisa Suyama, Chika Tada, Yasuhiro Fukuda, Masanori Saito, Yutaka Nakai

    Microbes and Environments　34　(4)　421-428　2019

    DOI： 10.1264/jsme2.ME19113 　

    ISSN： 1342-6311

    eISSN： 1347-4405

  55. Resistance to 6-methylpurine is conferred by defective adenine phosphoribosyltransferase in Tetrahymena Peer-reviewed

    Takahiko Akematsu, Andrew Findlay, Yasuhiro Fukuda, Ronald E. Pearlman, Josef Loidl, Eduardo Orias, Eileen P. Hamilton

    Genes　9　(4)　2018/04/01

    DOI： 10.3390/genes9040179 　

    ISSN： 2073-4425

  56. Clinical, pathological, and molecular data concerning Coenurus cerebralis in Sheep in Egypt Peer-reviewed

    Said Amer, Ahmed ElKhatam, Yasuhiro Fukuda, Lamia I. Bakr, Shereif Zidan, Ahmed Elsify, Mostafa A. Mohamed, Chika Tada, Yutaka Nakai

    Data in Brief　16　1-9　2018/02/01

    Publisher: Elsevier Inc.

    DOI： 10.1016/j.dib.2017.10.070 　

    ISSN： 2352-3409

  57. 嫌気性LCFA分解微生物群集添加による油脂のメタン発酵の効率化 Peer-reviewed

    松本悠暉, 馮夢佳, 福田康弘, 中井裕, 多田千佳

    日本畜産環境学会　2018

  58. Prevalence, genotyping and risk factors of Giardia duodenalis from dogs in Vietnam Peer-reviewed

    Sam Thi Nguyen, Yasuhiro Fukuda, Duc Tan Nguyen, Ha Thanh Dao, Duc Quyet Le, Khanh Linh Bui, Chika Tada, Yutaka Nakai

    Journal of Veterinary Medical Science　80　(1)　92-97　2018

    Publisher: Japanese Society of Veterinary Science

    DOI： 10.1292/jvms.17-0498 　

    ISSN： 1347-7439 0916-7250

  59. Prevalence and etiology of mastitis in dairy cattle in El Oro Province, Ecuador Peer-reviewed

    Said Amer, Fernando Lenin Aguilar Gálvez, Yasuhiro Fukuda, Chika Tada, Ivan Ludeña Jimenez, Wunster Favian Maza Valle, Yutaka Nakai

    Journal of Veterinary Medical Science　80　(6)　861-868　2018

    Publisher: Japanese Society of Veterinary Science

    DOI： 10.1292/jvms.17-0504 　

    ISSN： 1347-7439 0916-7250

  60. Prevalence and Identity of Taenia multiceps cysts "Coenurus cerebralis" in Sheep in Egypt Peer-reviewed

    Said Amer, Ahmed ElKhatam, Yasuhiro Fukuda, Lamia I. Bakr, Shereif Zidan, Ahmed Elsify, Mostafa A. Mohamed, Chika Tada, Yutaka Nakai

    ACTA TROPICA　176　270-276　2017/12

    DOI： 10.1016/j.actatropica.2017.08.012 　

    ISSN： 0001-706X

    eISSN： 1873-6254

  61. Pretreatment of lignocellulosic biomass by cattle rumen fluid for methane production: Bacterial flora and enzyme activity analysis Peer-reviewed

    Yasunori Baba, Yu Matsuki, Yumi Mori, Yoshihisa Suyama, Chika Tada, Yasuhiro Fukuda, Masanori Saito, Yutaka Nakai

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING　123　(4)　489-496　2017/04

    DOI： 10.1016/j.jbiosc.2016.11.008 　

    ISSN： 1389-1723

    eISSN： 1347-4421

  62. Monitoring of pathogenic microorganisms originating from nomadic livestock feces in the Tuul River basin of Mongolia Peer-reviewed

    Chika Tada, Tomoe Kotogaoka, Moe Takada, Yasuhiro Fukuda, Yutaka Nakai

    Journal of Animal Production Environment Science　2017

  63. Observation of the Electrode Surface Microbes in Microbial Fuel Cells

    Masaki UMETSU, Yasuhiro FUKUDA, Yutaka NAKAI, Chika TADA

    Proceedings of the 13th International Symposium on Integrated Field Science “Conservation and Utilization of Biodiversity”　13　50-50　2016/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  64. Pretreatment of Paper Sludge with Rumen Fluid to Enhance Biogas Production

    Shuhei TAKIZAWA, Yasunori BABA, Chika TADA, Yasuhiro FUKUDA, Yutaka NAKAI

    Proceedings of the 13th International Symposium on Integrated Field Science “Conservation and Utilization of Biodiversity　13　49-49　2016/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  65. Site-specific phosphorylation of Tau protein is associated with deacetylation of microtubules in mouse spermatogenic cells during meiosis Peer-reviewed

    Hiroki Inoue, Yuuki Hiradate, Yoshiki Shirakata, Kenta Kanai, Keita Kosaka, Aina Gotoh, Yasuhiro Fukuda, Yutaka Nakai, Takafumi Uchida, Eimei Sato, Kentaro Tanemura

    FEBS LETTERS　588　(11)　2003-2008　2014/05

    DOI： 10.1016/j.febslet.2014.04.021 　

    ISSN： 0014-5793

    eISSN： 1873-3468

  66. Infectivity of Cryptosporidium andersoni and Cryptosporidium muris to Normal and Immunosuppressive Cynomolgus Monkeys Peer-reviewed

    Koichi Masuno, Yasuhiro Fukuda, Masahito Kubo, Ryo Ikarashi, Takeshi Kuraishi, Shosaku Hattori, Junpei Kimura, Chieko Kai, Tokuma Yanai, Yutaka Nakai

    JOURNAL OF VETERINARY MEDICAL SCIENCE　76　(2)　169-172　2014/02

    DOI： 10.1292/jvms.13-0350 　

    ISSN： 0916-7250

    eISSN： 1347-7439

  67. Bovine coccidia: classification and pathogenicity

    Nakai Yutaka, Sato Rintaro, Fukuda Yasuhiro, Chika Tada

    Japanese Journal of Large Animal Clinics　5　(2)　134-135　2014

    Publisher: Japanese Society of Large Animal Clinics / Large Animal Clinic Research Association

    DOI： 10.4190/jjlac.5.134 　

    ISSN： 1884-684X

  68. Enhancing methane production during the anaerobic digestion of crude glycerol using Japanese cedar charcoal Peer-reviewed

    Ryoya Watanabe, Chika Tada, Yasunori Baba, Yasuhiro Fukuda, Yutaka Nakai

    BIORESOURCE TECHNOLOGY　150　387-392　2013/12

    DOI： 10.1016/j.biortech.2013.10.030 　

    ISSN： 0960-8524

    eISSN： 1873-2976

  69. First description of heterogeneity in 18S rRNA genes in the haploid genome of Cryptosporidium andersoni Kawatabi type Peer-reviewed

    Makoto Ikarashi, Yasuhiro Fukuda, Hajime Honma, Kenji Kasai, Yoshiyasu Kaneta, Yutaka Nakai

    VETERINARY PARASITOLOGY　196　(1-2)　220-224　2013/09

    DOI： 10.1016/j.vetpar.2012.12.053 　

    ISSN： 0304-4017

  70. Detection and genotyping of Cryptosporidium spp. in large Japanese field mice, Apodemus speciosus. International-journal Peer-reviewed

    Fumi Murakoshi, Yasuhiro Fukuda, Ryuma Matsubara, Yuuki Kato, Rintaro Sato, Takako Sasaki, Chika Tada, Yutaka Nakai

    Veterinary parasitology　196　(1-2)　184-8　2013/09/01

    DOI： 10.1016/j.vetpar.2013.02.011 　

    More details Close

    The genus Cryptosporidium, which is an obligate intracellular parasite, infects various vertebrates and causes a diarrheal disease known as cryptosporidiosis. Wild rodents are naturally infected with zoonotic Cryptosporidium; thus, they are potential reservoirs of the parasites. Mice are common rodents frequently found in agricultural areas and have many opportunities to contact other wild animals, livestock, and humans. Irrespective of the potential risk, there are few epidemiologic studies of Cryptosporidium in wild mice because of their low economic importance and the difficulty in conducting surveys. Hence, the species and genotypes of Cryptosporidium in wild mice living around various areas remain unclear. We investigated the species and genotype distribution and prevalence of Cryptosporidium in the large Japanese field mouse (Apodemus speciosus) in an agricultural site in Osaki, Miyagi Prefecture, Japan. In total, 15 mice were captured and examined in this study. By microscopic analysis, only one mouse (JFM 3) was determined to be Cryptosporidium-positive, while the parasite were detected in four mice (JFM 3, 6, 10, and 15) by a molecular approach using partial SSU rRNA gene sequences. Based on nucleotide sequence and phylogenetic analysis, the Cryptosporidium isolates were identified as C. ubiquitum (from JFM 10) and C. muris (from JFM 3 and 6). In contrast, the Cryptosporidium in JFM 15 was not identified as a known species or genotype and is therefore proposed as a novel genotype; the Naruko genotype. More molecular data are necessary to elucidate the taxonomic identity of this novel Cryptosporidium genotype. The C. muris Japanese field mouse genotypes showed marked divergence compared to that in a previous report. The large Japanese field mouse might thus represent a reservoir of multiple Cryptosporidium spp.

  71. Anaerobic digestion of crude glycerol from biodiesel manufacturing using a large-scale pilot plant: Methane production and application of digested sludge as fertilizer Peer-reviewed

    Yasunori Baba, Chika Tada, Ryoya Watanabe, Yasuhiro Fukuda, Nobuyoshi Chida, Yutaka Nakai

    BIORESOURCE TECHNOLOGY　140　342-348　2013/07

    DOI： 10.1016/j.biortech.2013.04.020 　

    ISSN： 0960-8524

    eISSN： 1873-2976

  72. Prevalence and molecular characterization of Cryptosporidium in ostriches (Struthio camelus) on a farm in central Vietnam Peer-reviewed

    Sam Thi Nguyen, Yasuhiro Fukuda, Chika Tada, Vu Vy Huynh, Duc Tan Nguyen, Yutaka Nakai

    Experimental Parasitology　133　(1)　8-11　2013/01

    DOI： 10.1016/j.exppara.2012.10.010 　

    ISSN： 0014-4894 1090-2449

  73. Improvement of methane production from waste paper by pretreatment with rumen fluid Peer-reviewed

    Yasunori Baba, Chika Tada, Yasuhiro Fukuda, Yutaka Nakai

    Bioresource Technology　128　94-99　2013

    Publisher: Elsevier Ltd

    DOI： 10.1016/j.biortech.2012.09.077 　

    ISSN： 1873-2976 0960-8524

  74. Biological pretreatment of cellulosic biomass with waste cattle rumen fluid for methane production Peer-reviewed

    Baba Y, Tada C, Fukuda Y, Nakai Y

    Proceedings of 214th Symposium on Sustainable Humanosphere　57　2012/12/11

  75. Prevalence of Fasciola in cattle and of its intermediate host Lymnaea snails in central Vietnam Peer-reviewed

    Sam Thi Nguyen, Duc Tan Nguyen, Thoai Van Nguyen, Vu Vy Huynh, Duc Quyet Le, Yasuhiro Fukuda, Yutaka Nakai

    TROPICAL ANIMAL HEALTH AND PRODUCTION　44　(8)　1847-1853　2012/12

    DOI： 10.1007/s11250-012-0147-8 　

    ISSN： 0049-4747

  76. Novel method of methane production using waste cattle rumen fluids Peer-reviewed

    Baba Y, Tada C, Fukuda Y, Nakai Y

    Proceedings of THE 15th AAAP Animal Science Congress　305　2012/11/26

  77. Prevalence and risk factors associated with Cryptosporidium oocysts shedding in pigs in Central Vietnam Peer-reviewed

    Sam Thi Nguyen, Hajime Honma, Thomas Geurden, Makoto Ikarash, Yasuhiro Fukuda, Vu Vy Huynh, Duc Tan Nguyen, Yutaka Nakai

    RESEARCH IN VETERINARY SCIENCE　93　(2)　848-852　2012/10

    DOI： 10.1016/j.rvsc.2012.01.007 　

    ISSN： 0034-5288

    eISSN： 1532-2661

  78. Programmed nuclear death and its relation to apoptosis and autophagy during sexual reproduction in Tetrahymena thermophila Invited Peer-reviewed

    Takahiko AKEMATSU, Takashi KOBAYASHI, Eriko OSADA, Yasuhiro FUKUDA, Hiroshi ENDOH, Ronald E, PEARLMAN

    The Japanese Journal of Protozoology　45　(1-2)　1-15　2012/09/12

    Publisher: Japan Society of Protistology

    DOI： 10.18980/jjprotozool.45.1-2_1 　

    ISSN： 0388-3752

    eISSN： 2189-6747

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    「国際共同研究」

  79. Molecular characterization of Cryptosporidium spp. in grazing beef cattle in Japan Peer-reviewed

    Fumi Murakoshi, Lihua Xiao, Ryuma Matsubara, Rintaro Sato, Yuuki Kato, Takako Sasaki, Yasuhiro Fukuda, Chika Tada, Yutaka Nakai

    VETERINARY PARASITOLOGY　187　(1-2)　123-128　2012/06

    DOI： 10.1016/j.vetpar.2011.12.011 　

    ISSN： 0304-4017

    eISSN： 1873-2550

  80. MOLECULAR IDENTIFICATION OF FASCIOLA SPP. (DIGENEA: PLATYHELMINTHES) IN CATTLE FROM VIETNAM Peer-reviewed

    S. Nguyen, S. Amer, M. Ichikawa, T. Itagaki, Y. Fukuda, Y. Nakai

    PARASITE　19　(1)　85-89　2012/02

    ISSN： 1252-607X

    eISSN： 1776-1042

  81. Molecular identification and phylogenetic analysis of Trypanosoma evansi from dromedary camels (Camelus dromedarius) in Egypt, a pilot study Peer-reviewed

    Said Amer, Oishi Ryu, Chika Tada, Yasuhiro Fukuda, Noboru Inoue, Yutaka Nakai

    ACTA TROPICA　117　(1)　39-46　2011/01

    DOI： 10.1016/j.actatropica.2010.09.010 　

    ISSN： 0001-706X

    eISSN： 1873-6254

  82. Identification of Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers Peer-reviewed

    Said Amer, Yasser Dar, Madoka Ichikawa, Yasuhiro Fukuda, Chika Tada, Tadashi Itagaki, Yutaka Nakai

    PARASITOLOGY INTERNATIONAL　60　(1)　5-12　2011/01

    DOI： 10.1016/j.parint.2010.09.003 　

    ISSN： 1383-5769

  83. Cryptosporidium genotypes and subtypes in dairy calves in Egypt (vol 169, pg 382, 2010) Peer-reviewed

    Said Amer, Hajime Honma, Makoto Ikarashi, Chika Tada, Yasuhiro Fukuda, Yoshihisa Suyama, Yutaka Nakai

    VETERINARY PARASITOLOGY　172　(3-4)　361-361　2010/09

    DOI： 10.1016/j.vetpar.2010.05.021 　

    ISSN： 0304-4017

    eISSN： 1873-2550

  84. Molecular analysis of Cryptosporidium parvum HNJ-1 isolated in Japan. Peer-reviewed

    Said Amer, Honma H, Ikarashi M, Tada C, Fukuda Y, Suyama Y, Nakai Y

    J. Vet. Med. Sci　72　(12)　1647-1649　2010

    DOI： 10.1292/jvms.10-0118 　

Show all ︎Show first 5

Misc. 73

  1. フィリピンのコウモリにおけるCryptosporidium属の分子疫学調査と人獣共通感染症のリスク評価

    許林, 福田康弘, 村越ふみ, アルビオラ フィリップ, マサンカイ ジョセフ, シェハタ アイマン, 大松勉, 伴戸寛徳, 藤井ひかる, 宇根有美, 嘉手苅将, 松山亮太, 加藤健太郎

    日本寄生虫学会大会プログラム・抄録集　94th (CD-ROM)　2025

    ISSN： 1348-4613

  2. フィリピンの野生コウモリにおけるCryptosporidiumの分子疫学調査

    許林, 福田康弘, 村越ふみ, アルビオラ フィリップ, マサンカイ ジョセフ, シェハタ アイマン, 大松勉, 伴戸寛徳, 藤井ひかる, 宇根有美, 嘉手苅将, 松山亮太, 加藤健太郎

    日本獣医学会学術集会講演要旨集　167th　2024

    ISSN： 1347-8621

  3. 脂質高含有廃水処理リアクターで優占化したCandidatus Cloacimonadota細菌の環状メタゲノムアセンブリゲノムの構築とその特徴

    櫻井莉久, 櫻井莉久, 福田康弘, 多田千佳

    日本微生物生態学会大会(Web)　37th　2024

  4. Development of oil-degrading microbial preparation using vinylidene chloride carrier (PVDC) for promoting anaerobic digestion

    櫻井莉久, 福田康弘, 多田千佳, 津田精一, 宮崎泰輔

    日本水環境学会年会講演集　56th　2022

  5. Identification of potent drug candidates among the traditional Chinese medicine compounds for the treatment of Cryptosporidiosis

    ビンカビル モハメドハッジャジュ, ビンカビル モハメドハッジャジュ, レクエンコ フランセスカガヤット, モハメド ジンヌルカティージャ, 渡邊仁奈, 渡邉謙一, 福田康弘, 伴戸寛徳, 暮地本宙己, 玄学南, 加藤健太郎

    日本寄生虫学会大会プログラム・抄録集　91st　2022

    ISSN： 1348-4613

  6. Aqueous extract ofPhyllanthus niruriprotects against severe malaria by blocking erythrocyte invasion and modulating the host immune response

    Jeje Temitope Olawale, Hironori Bando, Yasuhiro Fukuda, Ibukun Emmanuel Oluwafemi, Kentaro Kato

    2021/07/10

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2021.07.09.451735 　

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    ABSTRACT Plasmodium falciparumparasites are the major cause of malaria across Africa. Due to the appearance of multi-drug resistant parasites, new antimalarial drugs are needed. The medicinal plantPhyllanthus niruriis being used to treat fever and other symptoms of malaria in Nigeria; however, little is known about its antimalarial mechanisms. Here, we show that aqueous extract ofP. niruri(PE) has multiple antimalarial effects, including anti-parasitic and host immunomodulatory activity. We found that co-culture ofP. falciparumwith PE drastically reduced parasite number, but PE did not inhibit parasite development or rupture; rather, it blocked erythrocytes invasion. In addition, we identified Astragalin as one of the antimalarial compounds which are contained in PE. Moreover, we found that PE suppresses the inflammatory activity and apoptosis of immune cells (T cells) and astrocytes and neurons in the central nervous system (CNS). Furthermore, we confirmed that oral administration of PE to mice suppressed parasite growth, excessive inflammation, CNS dysfunction, and the development of experimental cerebral malaria in anin vivomurine malaria model. Our findings demonstrate that PE has multiple effects on malaria progression, targeting both parasite and host cells.

  7. 1型クリプトスポリジウムウイルス(CSpV1)を直接検出するための、分子的な高感度法の開発(Development of a sensitive molecular method for direct detection of Cryptosporidium parvum Virus type 1(CSpV1))

    Shehata Ayman Ahmed, 伴戸 寛徳, 福田 康弘, Bin Kabir Mohammad Hazzaz, 村越 ふみ, 伊藤 めぐみ, 加藤 健太郎

    日本獣医学会学術集会講演要旨集　163回　207-207　2020/10

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  8. Development of a highly sensitive method for the detection of Cryptosporidium parvum virus type 1 (CSpV1)

    SHEHATA Ayman Ahmed, SHEHATA Ayman Ahmed, 伴戸寛徳, 福田康弘, KABIR Mohammad Hazzaz Bin, 村越ふみ, 伊藤めぐみ, ESSA Mohamed Ibrahim, 加藤健太郎, 加藤健太郎

    日本寄生虫学会大会プログラム・抄録集　89th　2020

    ISSN： 1348-4613

  9. 凝結凝集法がルーメン微生物の回収効率および植物系バイオマス分解特性におよぼす影響

    瀧澤修平, 阿部憲一, 福田康弘, 多田千佳, 中井裕

    廃棄物資源循環学会研究発表会講演集(Web)　30th　2019

  10. ルーメン微生物群集による植物系バイオマス分解過程における多糖分解酵素活性の推移

    瀧澤修平, 浅野亮樹, 福田康弘, 多田千佳, 中井裕

    日本畜産学会大会講演要旨　125th　2019

    ISSN： 1342-4688

  11. P-32 Effects of rumen protozoa on the pretreatmen with rumen fluid and the biogas production from paper sludge

    TAKIZAWA Shuhei, TADA Chika, FUKUDA Yasuhiro, NAKAI Yutaka

    Proceedings of the Conference on Biomass Science　13　109-110　2018

    Publisher: The Japan Institute of Energy

    DOI： 10.20550/jiebiomassronbun.13.0_109 　

    ISSN： 2423-8333

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    <p>Because paper sludge discharged from the waste paper recycling process contains high levels of lignin and ash, it is not converted into biogas effectively during methane fermentation. The aim of this study was to improve the efficiency of biogas production from paper sludge by the pretreatment with rumen fluid. We performed the pretreatment of paper sludge with rumen fluid at 37°C for 6 hours. After pretreatment, methane fermentation was conducted at 35°C for 45 days. Pretreatment with rumen fluid hydrolyzed paper sludge and increased the VFAs concentration during the pretreatment process. Methane production from pretreated paper sludge was 12.1 % higher than that from untreated paper sludge. These results revealed that pretreatment with rumen fluid improved the efficiency of methane production from paper sludge. Next, we investigated the effects of protozoa on methane fermentation of paper sludge. Protozoa was removed from rumen fluid with sodium dodecyl sulfate, and the pretreatment and methane fermentation of paper sludge was conducted. We observed the positive relationship between the number of protozoa and methane gas production. The results indicated that protozoa possessed positive effects on methane production from paper sludge.</p>

  12. P-32 Effects of rumen protozoa on the pretreatmen with rumen fluid and the biogas production from paper sludge

    瀧澤修平, 多田千佳, 福田康弘, 中井裕

    バイオマス科学会議発表論文集(Web)　13　2018

    ISSN： 2423-8341

  13. 繊毛虫テトラヒメナの配偶核形成に関与するクロマチン再構成因子の探索

    福田康弘, 明松隆彦, 多田千佳, 中井裕, 中井裕

    日本分子生物学会年会プログラム・要旨集(Web)　41st　2018

  14. 繊毛虫テトラヒメナの配偶核形成に関わるクロマチン再構成因子の探索

    福田康弘, 明松隆彦, 多田千佳, 中井裕, 中井裕

    日本原生生物学会大会講演要旨集　51st　2018

  15. メタン生成菌をカソード触媒として利用した微生物燃料電池の基礎的研究

    梅津将喜, 簾内崇彰, 福田康弘, 中井裕, 高橋英志, 多田千佳

    日本水環境学会年会講演集　51st　2017

  16. ウシルーメン液によるメタン発酵前処理過程の微生物群集構造解析

    馬場保徳, 馬場保徳, 馬場保徳, 松木悠, 馬場(森)裕美, 馬場(森)裕美, 陶山佳久, 多田千佳, 福田康弘, 三宅克英, 齋藤雅典, 中井裕

    日本生物工学会大会講演要旨集　69th　2017

  17. 腸管試料の遺伝子解析に基づくウシコクシジウム原虫の種別寄生部位の同定

    大坪大亮, 金森眞紀, 大脇啓嗣, 柳井徳磨, 多田千佳, 福田康弘, 中井裕

    日本寄生虫学会大会プログラム・抄録集　86th　2017

    ISSN： 1348-4613

  18. モンゴル国放牧家畜および環境水を介した病原体伝播の検証

    高田萌, 琴賀岡朋絵, バルダン トマー, 福田康弘, 中井裕, 多田千佳

    日本寄生虫学会大会プログラム・抄録集　86th　2017

    ISSN： 1348-4613

  19. 担体表面の化学処理によるMethanothermobacter thermautotrophicus strainΔHの付着性向上

    梅津将喜, 簾内崇彰, 福田康弘, 中井裕, 高橋英志, 多田千佳

    日本微生物生態学会大会(Web)　2017　2017

  20. ウシルーメン液による植物バイオマス可溶化処理およびその後のメタン発酵過程の微生物群集構造解析

    馬場保徳, 馬場保徳, 馬場保徳, 松木悠, 馬場(森)裕美, 馬場(森)裕美, 陶山佳久, 多田千佳, 福田康弘, 三宅克英, 齋藤雅典, 中井裕

    日本微生物生態学会大会(Web)　2017　2017

  21. 家畜ふん堆肥に由来するアンモニア酸化古細菌の生理学的特性

    馬場(森)裕美, 馬場(森)裕美, 多田千佳, 福田康弘, 三宅克英, 馬場保徳, 中井裕

    日本微生物生態学会大会(Web)　2017　2017

  22. ウシコクシジウム原虫の種特異的な腸管内寄生部位の探索

    大坪大亮, 金森眞紀, 大脇啓嗣, 柳井徳磨, 多田千佳, 福田康弘, 中井裕

    日本原生生物学会大会講演要旨集　50th　2017

  23. DNAプローブを用いた腸管組織切片上のコクシジウム原虫の種同定と寄生部位の解析

    福田康弘, 金森眞紀, 大坪大亮, 大脇啓嗣, 多田千佳, 柳井徳磨, 中井裕

    日本原生生物学会大会講演要旨集　50th　2017

  24. ルーメン液の保存が植物系バイオマスのバイオガス化におよぼす影響

    瀧澤修平, 多田千佳, 福田康弘, 中井裕

    廃棄物資源循環学会研究発表会講演集(Web)　28th　2017

  25. ホルマリン固定試料と遺伝子解析法を用いたウシコクシジウムの種別検出および腸管内感染部位の同定

    金森眞紀, 曽地雄一郎, 西清志, 多田千佳, 福田康弘, 中井裕

    日本寄生虫学会大会プログラム・抄録集　85th　2016

    ISSN： 1348-4613

  26. 繊毛虫テトラヒメナの半数体核に生じるDNA二重鎖切断とメカニズム

    福田康弘, 明松隆彦, PEARLMAN Ronald E., 多田千佳, LOIDL Josef, 中井裕

    日本原生生物学会大会講演要旨集　49th　2016

  27. 放射性セシウム汚染作物からのセシウム溶出特性とウシルーメン微生物処理・ナノ磁性粒子による除染

    馬場保徳, 馬場保徳, 日尾彰宏, 並木禎尚, 多田千佳, 福田康弘, 三宅克英, 齋藤雅典, 中井裕

    日本生物工学会大会講演要旨集　68th　2016

  28. 硫黄添加堆肥におけるpH低下およびアンモニア揮散抑制に寄与する硫黄酸化細菌の生態

    森裕美, 多田千佳, 福田康弘, 中井裕

    日本微生物生態学会大会(Web)　31st　2016

  29. テトラヒメナの配偶核形成におけるDNA二重鎖切断とその役割について

    福田康弘, 明松隆彦, PEARLMAN Ronald E., 多田千佳, LOIDL Josef, 中井裕

    日本分子生物学会年会プログラム・要旨集(Web)　39th　2016

  30. Escherichia coli in Tuul River, Mongolia

    TAKADA Moe, TADA Chika, YOSHIHARA Yu, FUKUDA Yasuhiro, BALDAN Tuner, NAKAI Yutaka

    Journal of Integrated Field Science　12　83-83　2015/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  31. Methane Production Using Cattle Rumen Fluids and Its Application to Reduce Volume of Radiation-polluted Biomass

    BABA Yasunori, TADA Chika, FUKUDA Yasuhiro, SAITO Masanori, NAKAI Yutaka

    Journal of Integrated Field Science　12　85-85　2015/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  32. Enrichment and Characterization of an Ammonia-Oxidizing Archaeon of Moderate Thermophilic Taumarchaeotal Group I.1a from Cattle Manure Compost

    MORI Yumi, TOJO Fuyumi, FUKUDA Yasuhiro, TADA Chika, NAKAI Yutaka

    Journal of Integrated Field Science　12　84-84　2015/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  33. ウシの第一胃内容液を活用したメタン発酵と資源循環システム

    馬場保徳, 多田千佳, 福田康弘, 中井裕

    バイオサイエンスとインダストリー　73　(2)　141-145　2015/03/01

    Publisher: バイオインダストリー協会

    ISSN： 0914-8981

  34. ウシルーメン液による植物バイオマス可溶化処理過程の16S rRNAメタゲノム解析:endo-/exo-glucanaseおよびxylanase生産細菌の推定

    馬場保徳, 馬場保徳, 松木悠, 森裕美, 陶山佳久, 多田千佳, 福田康弘, 齋藤雅典, 中井裕

    日本生物工学会大会講演要旨集　67th　2015

  35. テトラヒメナの半数体核に生じるDNA切断とクロマチン再構成について

    福田康弘, 明松隆彦, PEARLMAN Ronald E., LOIDL Josef, 多田千佳, 中井裕

    日本原生生物学会大会講演要旨集　48th　2015

  36. 牛ふん堆肥化過程における硫黄酸化細菌の分子生態学的研究

    森裕美, 福田康弘, 多田千佳, 中井裕

    日本微生物生態学会大会(Web)　30th　2015

  37. ウシ・ルーメン微生物のセルラーゼおよびキシラナーゼ活性を利用したメタン発酵システムの開発(水処理生態系(waste water treatment),一般講演,環境微生物系学会合同大会2014講演要旨)

    馬場 保徳, 李 哲揆, 多田 千佳, 福田 康弘, 齋藤 雅典, 中井 裕

    土と微生物　68　(2)　99-99　2014/10/01

    Publisher: 日本土壌微生物学会

    ISSN： 0912-2184

  38. Prevalence and Molecular Characterization of Cryptosporidium in Domestic Animals in Central Vietnam

    Nguyen Sam Thi, Fukuda Yasuhiro, Tada Chika, Vy Huynh Vu, Nguyen Due Tan, Nakai Yutaka

    Journal of Integrated Field Science　11　82-82　2014/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  39. Prevalence and Molecular Identification of Fasciola in Cattle in Central Vietnam.

    Nguyen Sam Thi, Fukuda Yasuhiro, Tada Chika, Huynh Vu Vy, Nguyen Duc Tan, Nakai Yutaka

    Journal of Integrated Field Science　11　83-83　2014/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  40. Phylogenetic Analysis of E. zuernii and E. bovis with Nuclear 18S rRNA and Mitochondrial CO1 Genes

    Sato Rintaro, Takahashi Tomoya, Numazu Keiji, Wakatsuki Takushi, Morita Yasuhiro, Komura Akimasa, Tada Chika, Fukuda Yasuhiro, Nakai Yutaka

    Journal of Integrated Field Science　11　81-81　2014/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  41. Molecular Epidemiological Survey for Zoonotic Parasite Infection in Livestock in the Tuul Basin in Mongolia

    Kotogaoka Tomoe, Fukuda Yasuhiro, Tada Chika, Nakai Yutaka

    Journal of Integrated Field Science　11　84-84　2014/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  42. Host Immune Response to Gastric Cryptosporidium

    Chiba Yoshiki, Fukuda Yasuhiro, Tada Chika, Nakai Yutaka

    Journal of Integrated Field Science　11　80-80　2014/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  43. Pretreatment of Lignocellulosic Rapeseed Waste by Rumen Fluid for Methane Production

    Baba Yasunori, Lee Chol Gyu, Tada Chika, Fukuda Yasuhiro, Nakai Yutaka

    Journal of Integrated Field Science　11　89-89　2014/03

    Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University

  44. マウス精子発生過程におけるTauの動態解析

    井上 弘貴, 平舘 裕希, 白形 芳樹, 福田 康弘, 中井 裕, 内田 隆史, 種村 健太郎

    日本畜産学会大会講演要旨集　118回　110-110　2014/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  45. P22-28 牛糞コンポスト由来アンモニア酸化古細菌集積培養系における微生物生態学的研究(ポスター発表)

    森 裕美, 東條 ふゆみ, 山本 希, 大石 竜, 多田 千佳, 福田 康弘, 中井 裕

    日本微生物生態学会講演要旨集　2014　224-224　2014

    Publisher: 日本微生物生態学会

  46. ウシ・ルーメン液によるリグノセルロース分解特性の解析とメタン発酵への応用

    馬場保徳, 馬場保徳, 多田千佳, LEE Chol Gyu, 福田康弘, 中井裕

    バイオマス科学会議発表論文集　9th　2014

  47. ウシルーメン液を用いたリグノセルロース系バイオマス前処理の連続試験におけるpH低下が微生物群集構造に与える影響

    LEE Chol Gyu, 馬場保徳, 福田康弘, 多田千佳, 中井裕

    バイオマス科学会議発表論文集　9th　2014

  48. モンゴルにおける放牧家畜および周辺水環境からの病原性微生物の検出

    琴賀岡朋絵, 福田康弘, 多田千佳, 中井裕

    日本水環境学会年会講演集　48th　2014

  49. 牛糞コンポスト由来アンモニア酸化古細菌集積培養系における微生物生態学的研究

    森裕美, 東條ふゆみ, 山本希, 大石竜, 多田千佳, 福田康弘, 中井裕

    日本土壌微生物学会講演要旨集　2014　2014

  50. ルーメン液による草本メタン発酵前処理およびその後のメタン発酵におけるリグニン分解特性

    馬場保徳, 馬場保徳, 多田千佳, 福田康弘, 中井裕

    日本生物工学会大会講演要旨集　66th　2014

  51. 牛ルーメン液を用いた古紙分解に関わる微生物群の解明

    李哲揆, 浅野亮樹, 稲元民夫, 馬場保徳, 多田千佳, 福田康弘, 中井裕

    日本土壌微生物学会講演要旨集　2014　2014

  52. ウシ・ルーメン微生物のセルラーゼおよびキシラナーゼ活性を利用したメタン発酵システムの開発

    馬場保徳, 馬場保徳, 李哲揆, 多田千佳, 福田康弘, 齋藤雅典, 中井裕

    日本土壌微生物学会講演要旨集　2014　2014

  53. ウシのルーメン液を使用した前処理がメタン発酵のセルラーゼおよびキシラナーゼ活性に及ぼす効果

    馬場保徳, 馬場保徳, 多田千佳, 福田康弘, 中井裕

    廃棄物資源循環学会研究発表会講演集(Web)　25th　2014

  54. 動物のクリプトスポリジウム病

    中井裕, 加藤佑樹, 福田康弘

    宮城県獣医師会誌　123-128　2013/06

  55. 人獣共通感染症としてのクリプトスポリジウム症

    中井裕, 福田康弘

    G.I.Research　21　(6)　28-35　2013/06

  56. モンゴルにおける放牧家畜の原虫感染状況と水質汚染状況

    琴賀岡朋絵, 福田康弘, 多田千佳, 中井裕

    日本獣医学会学術集会講演要旨集　156th　2013

    ISSN： 1347-8621

  57. テトラヒメナの配偶核における新奇なγH2AX形成

    明松隆彦, 福田康弘, 多田千佳, 中井裕, PEARLMAN Ronald E.

    日本原生動物学会プログラム講演要旨　46th　2013

  58. ウシ抗菌ペプチドBMAP-28のホモロジーモデリングによる構造解析と抗腫瘍活性

    黒田 健吾, 磯貝 恵美子, 高木 志秋, 加藤 裕樹, 福田 智一, 米山 裕, 安藤 太助, 中井 裕, 福田 康弘

    日本畜産学会大会講演要旨集　115回　247-247　2012/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  59. ウシ腸管上皮細胞を用いたウシコクシジウムの培養

    須藤佳子, 麻生久, 多田千佳, 福田康弘, 中井裕

    日本獣医学会学術集会講演要旨集　154th　2012

    ISSN： 1347-8621

  60. CathelicidinによるCryptosporidium殺滅と感染細胞のアポトーシス誘導

    加藤佑樹, 磯貝恵美子, 多田千佳, 福田康弘, 中井裕

    日本獣医学会学術集会講演要旨集　154th　2012

    ISSN： 1347-8621

  61. コクシジウム症を呈したウシから分離したオーシストの形態および遺伝子解析

    佐藤臨太郎, 高橋知也, 沼津敬治, 多田千佳, 福田康弘, 中井裕

    日本獣医学会学術集会講演要旨集　154th　2012

    ISSN： 1347-8621

  62. Crane coccidiosis

    中井裕, 本間一, 福田康弘

    動物の原虫病　26　(1)　7-11　2011/12/15

    Publisher: 動物の原虫病研究会

    ISSN： 0915-7506

  63. Molecular taxonomy of Eimeria sp. from pigeon (Columba livia domestica) in Japan

    松原立真, 福田康弘, 村越ふみ, 中井裕

    原生動物学雑誌　44　(1)　2011

    ISSN： 0388-3752

  64. Molecular epidemiology of bovine coccidiosis in northern Miyagi prefecture, Japan

    佐藤臨太郎, 村越ふみ, 高橋知也, 沼津敬治, 福田康弘, 中井裕

    原生動物学雑誌　44　(1)　2011

    ISSN： 0388-3752

  65. 放牧農場におけるクリプトスポリジウム原虫の分子疫学調査

    村越ふみ, 松原立真, 佐藤臨太郎, 加藤祐樹, 佐々木貴子, 福田康弘, 多田千佳, 中井裕

    日本獣医学会学術集会講演要旨集　152nd　2011

    ISSN： 1347-8621

  66. 消毒土壌におけるアンモニア酸化真正細菌および古細菌

    木村久美, 山本希, 多田千佳, 福田康弘, 中井裕

    土と微生物　65　(2)　2011

    ISSN： 0912-2184

  67. 実規模施設の牛ふんコンポスト化過程におけるアンモニア酸化古細菌群集の解析

    山本希, 大石竜, 浅野亮樹, 吉井啓貴, 福田康弘, 多田千佳, 中井裕

    日本畜産学会大会講演要旨　112th　2010

    ISSN： 1342-4688

  68. 家畜糞コンポスト化過程に存在するアンモニア酸化菌の培養試験

    大石竜, 浅野亮樹, 多田千佳, 三宅英成, 山本希, 福田康弘, 陶山佳久, 中井裕

    日本水環境学会年会講演集　44th　2010

  69. Cryptosporidium不活化に対する光触媒装置およびオゾン水の実用効果

    松原立真, 遠藤幹子, 村越ふみ, 本間一, 福田康弘, 多田千佳, 中井裕

    日本寄生虫学会大会プログラム・抄録集　79th　2010

    ISSN： 1348-4613

  70. マウス腸管上皮(MIE)細胞によるCryptosporidium parvum感染性評価方法の確立

    村越ふみ, 遠藤幹子, 松原立真, 本間一, 福田康弘, 多田千佳, 麻生久, 金谷高史, 中井裕

    日本寄生虫学会大会プログラム・抄録集　79th　2010

    ISSN： 1348-4613

  71. Cryptosporidium andersoniの遺伝子型解析および他宿主への感染能評価

    五十嵐亮, 福田康弘, 河西健二, 本間一, 金田善靖, 久保正仁, 服部正策, 柳井徳磨, 倉石武, 甲斐千恵子, 多田千佳, 中井裕

    日本寄生虫学会大会プログラム・抄録集　79th　2010

    ISSN： 1348-4613

  72. Complete life cycle of the red-tide dinoflagellate Noctiluca scintillans (ehrenberg) McCartney

    Yasuhiro Fukuda, Hiroshi Endoh

    ZOOLOGICAL SCIENCE　23　(12)　1160-1160　2006/12

    ISSN： 0289-0003

  73. ヤコウチュウの一生

    福田康弘, 遠藤浩

    うみうし通信　53　(53)　2-4　2006/12

    Publisher: 無脊椎動物研究所

    ISSN： 1341-5972

Show all ︎Show first 5

Books and Other Publications 4

  1. 原生生物学事典

    福田康弘

    朝倉書店　2023/05

    ISBN: 9784254171815

  2. 「アメーバのはなし -原生生物・人・感染症-」

    福田 康弘, 中井 裕

    朝倉書店　2018/09

  3. 人獣共通感染症第３版

    中井裕, 福田康弘

    医薬ジャーナル　2016/03/01

  4. Marine Protists

    Yasuhiro Fukuda, Toshinobu Suzaki

    Springer Japan　2015/10

    DOI： 10.1007/978-4-431-55130-0_2 　

    ISBN: 9784431551294

Presentations 37

  1. Effects of Fixation Methods on the Preservation of Subcellular Ultrastructure in the Dinoflagellate Oxyrrhis marina Invited

    Yasuhiro Fukuda

    2025/11/01

  2. Functional Analysis of the Macronuclear Pore Complex Associating Protein Mana1 in Tetrahymena thermophila

    FUKUDA Yasuhiro, IWAMOTO Masaaki, AKEMATSU Takahiko, KATO Kentaro

    2025/09/28

  3. Identification and characterization of Snf2 superfamily proteins involved in the early stages of conjugation in Tetrahymena thermophila Invited

    Yasuhiro Fukuda

    2025 Ciliate Molecular Biology Conference　2025/07/27

  4. Effect of Fixation Methods on Protist Ultrastructure: Freeze-Substitute vs. Conventional Chemical Fixation Invited

    Yasuhiro FUKUDA

    16th International Congress of Protistology 2025　2025/06/26

  5. 繊毛虫テトラヒメナの有性生殖における Snf2 の機能 Invited

    福田康弘

    第94回日本寄生虫学会大会 原生生物学共生生物学談話会　2025/03/17

  6. 繊毛虫テトラヒメナの配偶核形成における Semi1 と Semi2 タンパク質の協調作用

    明松隆彦, 福田康弘, 岩本政明

    第55回 日本原生生物学会大会　2022/09/02

  7. 繊毛虫テトラヒメナの二価染色体形成における Snf2 様タンパク質の役割

    福田康弘, 明松隆彦, 岩本政明, 伴戶寛徳, 加藤健太郎

    第44回日本分子生物学会年会　2021/12/01

  8. テトラヒメナの二価染色体形成における減数第一分裂前期特異的タンパク質 Mep1 の役割

    福田 康弘, 明松 隆彦, 岩本 政明, 伴戸 寛徳, 加藤 健太郎

    第 54 回 日本原生生物学会大会　2021/11/22

  9. Molecular mechanisms and predicted biological functions for the novel post-meiotic DSBs formation in the protist Tehetarhymena thermophila Invited

    Yasuhiro Fukuda

    MBSJ2020　2020/12/02

  10. Meiosis specific Snf2 chromatin remodeler in Tetrahymena possesses a pivotal role to generate gametic nuclei

    2019/10/26

  11. 繊毛虫テトラヒメナで見いだされた配偶核形成に不可欠な DNA 切断

    福田康弘, 明松隆彦

    第2回原生生物・寄生虫・進化セミナー　2018/11/28

  12. 配偶核形成から発見された新奇なDNA二重鎖切断の分子メカニズムとその役割

    福田康弘, 明松隆彦, 多田千佳, 中井裕

    第41回日本分子生物学会年会　2018/11/28

  13. 繊毛虫テトラヒメナの配偶核形成に関わるクロマチン再構成因子の探索

    福田康弘, 明松隆彦, 多田千佳, 中井裕

    第51回日本原生生物学会大会　2018/10/20

  14. 二核性生物テトラヒメナの大小核核膜孔複合体の違い

    岩本, 小坂田, 森, 長尾, 小布施, 平岡, 原口

    2017年度生命科学系学会合同年次大会（ConBio2017）　2017/12/06

  15. DNA プローブを用いた腸管組織切片上のコクシジウム原虫の種同定と寄生部位の解析

    金森 眞紀, 大坪 大亮, 大脇 啓嗣, 多田 千佳, 柳井 徳磨, 中井 裕

    第50回日本原生生物学会大会 第1回日本共生生物学会大会　2017/11/17

  16. ウシコクシジウム原虫の種特異的な腸管内寄生部位の探索

    大坪大亮, 金森眞紀, 大脇啓嗣, 柳井徳磨, 多田千佳, 中井裕

    第50回日本原生生物学会大会 第1回日本共生生物学会大会　2017/11/17

  17. 腸管試料の遺伝子解析に基づくウシコクシジウム原虫の種別寄生部位の同定

    大坪大亮, 金森眞紀, 大脇啓嗣, 柳井徳磨, 多田千佳, 福田康弘, 中井裕

    第86回日本寄生虫学会大会　2017/05/27

  18. モンゴル国放牧家畜および環境水を介した病原体伝播の検証

    高田 萌, 琴賀岡朋絵, バルダン トマー, 福田 康弘, 中井 裕, 多田 千佳

    第86回日本寄生虫学会大会　2017/05/27

  19. 渦鞭毛藻類 Oxyrrhis marina の染色体の３D構造解析

    洲崎敏伸, ソンチホン, 村田和義

    日本藻類学会第41回大会　2017/03/24

  20. A novel formation of double strand DNA breaks in the haploid germline nuclei of Tetrahymena thermophila

    福田 康弘, 明松 隆彦, Ronald E. Pearlman, 多田 千佳, Josef Loidl, 中井 裕

    第39回日本分子生物学会年会　2016/11/30

  21. 3-dimentional analysis of dinoflagellate nucleus by electron microscopy

    Yasuhiro Fukuda, Toshinobu Suzaki, Akihito Mori, Chihong Song, Kazuyoshi Murata

    生体ボリュームイメージ＆生理研研究会　2016/11/16

  22. 繊毛虫テトラヒメナの半数体核に生じる DNA 二重鎖切断とメカニズム

    明松 隆彦, Ronald E. Pearlman, 多田 千佳, Josef Loidl, 中井 裕

    第 49 回日本原生生物学会大会　2016/10/08

  23. ホルマリン固定試料と遺伝子解析法を用いたウシコクシジウムの種別検出および腸管内感染部位の同定

    金森眞紀, 曽地雄一郎, 西清志, 多田千佳, 中井裕

    寄生虫学会　2016/03/19

  24. テトラヒメナの半数体核に生じるDNA切断とクロマチン再構成について

    明松 隆彦, Ronald E. Pearlman, Josef Loidl, 多田 千佳, 中井 裕

    第48回日本原生生物学会大会　2015/11/06

  25. 3-dimentional analysis of dinoflagellate nucleus by electron microscopy International-presentation

    Chihong Song, Toshinobu Suzaki

    VII ECOP-ISOP Joint Meeting　2015/09/05

  26. Developmental transition of Histone H4 acetylations in micronuclei during early stage of conjugation of Tetrahymena thermophila International-presentation

    Takahiko Akematsu, Chika Tada, Yutaka Nakai

    Ciliate Molecular Biology 2015　2015/07/10

  27. A novel type of γ-H2AX formation in postmeiotic micronuclei and its relation to nuclear reprogramming in Tetrahymena thermophila International-presentation

    Takahiko Akematsu, Jyoti Garg, Jeffrey S. Fillingham, Ronald E. Pearlman, Josef Loidl

    Ciliate Molecular Biology 2015　2015/07/10

  28. いかにして繊毛虫の世代交代は運命づけられるのか？ Invited

    福田康弘, 明松隆彦, Ronald E. Pearlman

    日本動物学会第85回大会　2014/09/11

  29. 繊毛虫テトラヒメナの世代交代を約束する配偶核ゲノムのヒストン翻訳後修飾 Invited

    マトリョーシカ型生物学研究会　2014/07/11

  30. 環境 DNA や環境 RNA のメタゲノム解析による土壌原生生物の生態系の解明と新規分類群の探索

    Yasuhiro Fukuda, Satoshi Shimano

    発酵研究所第８回助成研究報告会　2014/06/05

  31. モデル原生生物のテトラヒメナを用いたプログラム核死に関わる分子制御の解明と生物の死を定めるシステムの探求 International-presentation

    若手SAP国際シンポジウム　2013/02/28

  32. ウイルスが真核生物の進化に与えるインパクト “渦鞭毛虫核は NCLDV が作り上げたのか？”

    中井 裕, 洲崎 敏伸

    日本原生動物学会第44回大会　2011/11/11

  33. Identification and Characterization of chromosomal protein in histone less eukaryote Oxyrrhis marina: virus infection might play a important role for dinoflagellate evolution International-presentation

    Tsohinobu SUZAKI

    International society for evolutionary protistology　2010/07/02

  34. 渦鞭毛虫の染色体構造解明に向けて：新規染色体タンパク質Np23 の同定と解析

    日本原生動物学会第42回大会　2009/10/30

  35. Nuclear proteins and chromosome structures of the ancestral dinoflagellate Oxyrrhis marina International-presentation

    Toshinobu SUZAKI

    International Congress of Protistology　2009/08/23

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    査読あり

  36. NEW DETAILS OF THE WHOLE LIFE CYCLE AND GAMETE MORPHOLOGY OF THE RED TIDE DINOFLAGELLATE NOCTILUCA SCINTILLANS (EHRENBERG) MCCARTNEY International-presentation

    Hiroshi ENDOH

    International Congress of Protistology　2009/08/23

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    査読あり

  37. 渦鞭毛虫の染色体構造の解明へ向けて（オキシリスの染色体タンパク質 Np23の解析）

    洲崎敏伸

    第４回日本進化原生生物研究会　2009/07/04

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Industrial Property Rights 2

  1. DNAプローブを用いたウシコクシジウム原虫の包括的および種特異的検出法

    中井裕, 福田康弘, 佐々木貴子, 多田千佳, 金森眞紀

    Property Type: Patent

  2. ルーメン液によるセルロース含有廃棄物を用いた有機酸発酵方法

    Yutaka Nakai, Yasunori Baba, Chika Tada, Yasuhiro Fukuda

    特許第5920728号

    Property Type: Patent

Research Projects 10

  1. 渦鞭毛藻だけがもつヒストンタンパク質非依存的クロマチンについて，その分子機構と成立に至る進化の解明

    Offer Organization: 公益財団法人 発酵研究所

    System: 一般研究助成

    2025/04 - 2027/03

  2. 未培養ルーメン微生物による非食用バイオマスの分解機構の解明とメタン発酵の高効率化

    中井 裕, 浅野 亮樹, 阿部 憲一, 馬場 保徳, 多田 千佳, 福田 康弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(A)

    Category: 基盤研究(A)

    Institution: 東北大学

    2021/04 - 2024/03

  3. 配偶核形成に必須の新奇なゲノム切断について，クロマチン再構築を伴う修復機構の研究

    福田康弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2021/04 - 2023/03

  4. Methane fermentation of inedible biomass by highly concentrated and compacted functional rumen microbial communities

    Yutaka Nakai

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    2017/04/01 - 2021/03/31

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    After the treatment of crop residues or waste paper by cattle rumen fluid, methane fermentation of these materials was accelerated. Enzymes and microbial communities in the process was investigated. Fiber‐degrading cellulase was diverse in the process; however, specific enzymes of xylanase showed high enzymatic activity continuously. Cellulase activity in the rumen treatment tank was continued for more than one month. At least five types of cellulases having different molecular sizes were existed. Microbes producing or involving these cellulases were unclassified species and not dominant in the microbial community.

  5. Molecular mechanisms of chromatin remodeling for the gamete-nuclei formation in Tetrahymena thermophila

    Yasuhiro Fukuda

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04 - 2021/03

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    In the ciliate Tetrahymena, the gamete-nuclei formation involves chromatin remodeling induced by a novel DNA double-strand break at the post-meiosis stage. Chromatin remodeling in the pronucleus during the gamete-nuclei formation is an evolutionarily conserved phenomenon. This occurs not only in ciliate but also in animals such as humans. However, the molecular mechanism of this phenomenon has been obscure. This study focused on the chromatin remodelers involved in the euchromatin formation for the gamete-nuclear formation and the changes in the epigenomic state associated with the gamete-nuclear formation. We identified some proteins involved in euchromatin formation and unclosed some parts in the system that controls it.

  6. A novel formation of double-strand DNA breaks in the haploid germline nuclei of Tetrahymena thermophila. Study for its molecular mechanisms and biological roles

    Yasuhiro Fukuda, AKEMATSU Takahiko, PEARLMAN E. Ronald

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2015/04 - 2018/03

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    In this study, molecular mechanisms and biological roles of a novel formation of post-meiotic DNA double-strand breaks (DSB) in the haploid micronuclei (pronuclei) of Tetrahymena thermophila were analyzed. After completion of meiosis, the TopII, and Spo11 mediated DSB formation in all the four pronuclei. Soon thereafter, DNA repair including histone replacement took place in the pronucleus that was adjacent to the mating-junction. This nucleus then began an additional mitosis as the selected pronucleus to produce gametic nuclei, while the other three unselected nuclei with persistent DSB eventually disappeared from the cytoplasm. Defective DSB formation in mutant strains abolishes not only nuclear selection but also histone replacement. These results suggest that the post-meiotic DSB is likely an intrinsic developmental program of the pronucleus and potentially contributes to chromatin remodeling to produce gametic nuclei capable of forming a zygotic nucleus.

  7. 環境 DNA や環境 RNA に基づく原生生物の分子生態系解析による、多様な土壌環境とその周辺水域における生態系の解明、および新規分類群の探索

    Offer Organization: 公益財団法人 発酵研究所

    System: 一般研究助成

    2012/04 - 2014/03

  8. Taxonomic investigation on the protozoan fauna of Japan, with special reference to the diversity crisis due to global climate change.

    SHIGENAKA Yoshinobu, SUZAKI Toshinobu, HAGA Nobuyuki, TSUKII Yuji, HORIGUCHI Tateo, ANDO Motonori, OTA Takashi, TANIGUCHI Akira, FUKUDA Yasuhiro, SUGIURA Mayumi, ISHIDA Hideki, SHIMANO Satoshi, HARUMOTO Terue, KITADE Osamu, KUSUOKA Yasushi, TAKAHASHI Tadao, IMAI Soichi, FUJISHIMA Masahiro, HORI Manabu, MATSUOKA Tatsuomi, ISHIDA Masaki, TAKAHASHI Mihoko, SUGIURA Mayumi, INAI Yoko, SUETOMO Yasutaka, AOKI Yoshiyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Hiroshima University

    2009 - 2012

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    Up to the present time, protozoan fauna in Japan has not been fully documented. Therefore, we, more than 20 protozoan specialists, have conducted a systematic survey on the Japanese protozoans by 1) collecting original bibliographic records 2) listing up species names and collection points, and 3) extracting reliable list of literature, in order to construct a comprehensive inventory database of Protozoan fauna of Japan. We have also conducted a nation -wide field survey of free-living and parasitic protozoans at more than 100 locations in Japan, including Abashiri area in Hokkaido and Minami Daito Island in Okinawa prefecture. The result of the survey has been presented on line at http://sites.google.com/site/protistsurvey/.

  9. 有機性廃棄物処理で生成されたコンポストおよび消化液の施肥による土壌生態系への影響と安全で安定した使用方法の検討

    Offer Organization: 公益財団法人 日本生命財団

    System: 環境問題研究 若手研究助成

    2010/05 - 2011/03

  10. 原生生物の進化と多様性 Competitive

    System: The Other Research Programs

    2010/04 -

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Other 4

  1. 環境DNAや環境RNAに基づく原生生物の分子生態系解析による、多様な土壌環境とその周辺水域における生態系の解明、および新規分類群の探索

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    本研究では、畑や水田、森林、草原、河川など様々な環境がある広大な東北大学農学研究科附属複合生態フィールド教育研究センター（以下FSC）に、主な複数のスタディサイトを儲ける。これにより、ほぼ同一の気候条件の異なる環境で生息する原生生物の生態構造をとらえられ、本研究の主要な目的である土壌周辺を含む生態系での原生生物の流れを詳しく解析できる。また、原生生物の探索は、環境DNAのライブラリによる分子生物学的手法を基軸に行い、その類縁解析は、最新で再精密の分子系統解析を用いる。また、原生生物の生理活性は、mRNAによる環境RNA解析を用いる。ここでは、DNA複製酵素など分裂と関わる分子の遺伝子、あるいはHSPなどの恒常的発現遺伝子などの遺伝子量と、またそれらの系統解析により、生態系で活発な原生生物種を同定できる。

  2. 排熱活用小型メタン発酵による分散型エネルギー生産と地域循環システムの構築

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    水産地域で排出される有機性廃棄物は水分を多く含むため、焼却に多大なエネルギーを要する。このような廃棄物の有効処理法としてメタン発酵がある。メタン発酵は嫌気性微生物の発酵によるメタンガス生産であるが、微生物活性を維持するために、反応槽を加温する必要がある。 これまでメタン発酵は、投入エネルギーと生産エネルギーの収支をプラスにするため、大規模システムが当然とされた。しかし、初期投資が数十億円と莫大であり、原料となる有機性廃棄物の調達コストが高い、原料不足が問題となっている。さらに、維持費も大きく、建設しても持続的維持が不可能になる課題があった。東北地方の沿岸域はリアス式海岸で、それぞれの港を含む水産地域は、小さな集落でまとまっているため、従来の大規模システムの導入は不適である。 本研究では、これまでメタン発酵で課題となっていた加温の投入エネルギーを水産加工業からの（煮蛸やしらすをゆでる時、乾燥,冷蔵冷凍時に無駄にされる）排熱を活用し、メタン発酵装置を温め、これまで不可能とされていたメタン発酵槽の小型化・省エネルギー化を可能にし、より安価で簡便にメタン発酵装置を小規模地域に導入し、地域内で水産地域由来の廃棄物系バイオマス処理をかねた分散型エネルギー生産を可能にすることを目的としている。 加えて、地域からの廃棄物を見える形で循環化し、町からの海への環境負荷を省コストに減らし、災害に強く、海を守りながら、低炭素型の持続可能な生活を可能にする町づくりを目的としている。 これにより、水産地域では、これまで廃棄物処理に係った処理コストを削減し、自立的エネルギー生産を可能になるだけでなく、いざというときの緊急電源および廃棄物処理システムを常備した町づくりにするものである。

  3. 有機性廃棄物処理で生成されたコンポストおよび消化液の施肥による土壌生態系への影響と、安全で安定した使用方法の検討

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    生活や生産で排出された有機性廃棄物の新たな処理方法として、微生物を利用した省エネルギーの処理が確立しつつあり、また生成品であるコンポストや消化液には肥料としての価値が期待されている。これら生成品について多くの研究が行われ、肥料として一定の評価が与えられた。だが、これら生成資材は莫大な微生物を含有し、投入による土壌生態系の撹乱は不可避と想定される。しかし生態系に対する影響、特に土壌の原生生物へ注目した研究はない。 本研究は、コンポストや消化液を肥料成分とした視点でなく莫大な微生物の集合体と見なし、使用に伴う土壌生態系の変化を、特に原生生物を中心に観る。解析は、生息する原生生物を直接観察する手法、土壌のDNAやRNA抽出による網羅的解析、また構築モデル系によるメタボローム解析など、新旧の生物学的手法を用いて行い、微生物資材の与えるミクロな生態系への影響を評価する。

  4. 生物学事典執筆