Details of the Researcher

PHOTO

Takahiro Eitsuka
Section
Graduate School of Agricultural Science
Job title
Associate Professor
Degree

  • 博士（農学） (Tohoku University)

Professional Memberships 9

  • 日本食品科学工学会

  • 日本食品分析学会

  • 日本過酸化脂質抗酸化物質学会

  • 日本脂質生化学会

  • 日本メイラード学会

  • 日本油化学会

  • 日本ビタミン学会

  • 日本栄養・食糧学会

  • 日本農芸化学会

︎Show all ︎Show first 5

Research Interests 2

  • 栄養生化学

  • 食品機能学

Research Areas 1

  • Life sciences / Food sciences /

Awards 5

  1. 奨励賞

    2020/06　日本ビタミン学会　トコトリエノールのがん抑制作用とその高機能化に関する研究

  2. オレオサイエンス賞

    2018/09　日本油化学会　こめ油成分を活用した相乗的ながん抑制効果

  3. 栄養・食糧学会奨励賞

    2017/05　日本栄養・食糧学会　脂溶性栄養成分による癌抑制に関する食品学的研究

  4. ICRBO2016 Best Oral Presentation Award

    2016/09　International Association of Rice Bran Oil

  5. 日本農芸化学会東北支部若手奨励賞

    2005/10　日本農芸化学会東北支部

Papers 64

  1. Engineering Immortalized Bovine Granulosa Cells Using a Triple‐Gene Approach: Mutant CDK4, Cyclin D1, and TERT

    Lanlan Bai, Minami Takahashi, Jin Kobayashi, Takahiro Eitsuka, Himari Matsusaka, Taku Ozaki, Eriko Sugano, Hiroshi Tomita, Yuan Xu, Kazuhiro Kawamura, Kiyotaka Nakagawa, Tohru Kiyono, Tomokazu Fukuda

    Cell Biology International　50　(2)　2026/01/21

    Publisher: Wiley

    DOI： 10.1002/cbin.70126 　

    ISSN： 1065-6995

    eISSN： 1095-8355

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    ABSTRACT Advancing reproductive technologies in livestock is essential to improve both productivity and genetic potential of cattle. Despite this importance, application of reproductive biotechnologies in cattle breeding remains limited. Bovine granulosa cells (bGCs), which are key components of the ovarian follicle, are critical in female reproduction as they produce steroid hormones and growth factors necessary for oocyte development. However, primary bGCs exhibit restricted proliferative capacity in vitro, limiting their utility in large‐scale studies on mechanisms related to follicular development. To address this limitation, we attempted to immortalize bGCs by co‐expressing human mutant cyclin‐dependent kinase 4 (CDK4 R24C ), cyclin D1, and telomerase reverse transcriptase (TERT) using lentiviral vectors. The resulting immortalized cells (bGCs‐K4DT) displayed extended proliferative lifespans, surpassing 100 population doublings without exhibiting signs of senescence. The transduced cells demonstrated a more active cell cycle profile and higher telomerase activity relative to parental bGCs. Importantly, they retained the bGC‐specific marker, aromatase, albeit at reduced expression levels. This immortalized bGC offers a promising model for investigating the role of bioactive components of platelet‐rich plasma (PRP) in follicular activation and growth, thereby supporting innovations in livestock reproductive technologies.

  2. 1‐Deoxynojirimycin attenuates pathological markers of Alzheimer's disease in the in vitro model of neuronal insulin resistance

    Isabella Supardi Parida, Soo Takasu, Junya Ito, Takahiro Eitsuka, Kiyotaka Nakagawa

    The FASEB Journal　38　(13)　2024/07/09

    Publisher: Wiley

    DOI： 10.1096/fj.202302600r 　

    ISSN： 0892-6638

    eISSN： 1530-6860

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    Abstract Insulin resistance, the hallmark of type 2 diabetes mellitus (T2DM), has emerged as a pathological feature in Alzheimer's disease (AD). Given the shared role of insulin resistance in T2DM and AD, repurposing peripheral insulin sensitizers is a promising strategy to preserve neuronal insulin sensitivity and prevent AD. 1‐Deoxynojirimycin (DNJ), a bioactive iminosugar, exhibited insulin‐sensitizing effects in metabolic tissues and was detected in brain tissue post‐oral intake. However, its impact on brain and neuronal insulin signaling has not been described. Here, we investigated the effect of DNJ treatment on insulin signaling and AD markers in insulin‐resistant human SK‐N‐SH neuroblastoma, a cellular model of neuronal insulin resistance. Our findings show that DNJ increased the expression of insulin signaling genes and the phosphorylation status of key molecules implicated in insulin resistance (Y1146‐pIRβ, S473‐pAKT, S9‐GSK3B) while also elevating the expression of glucose transporters Glut3 and Glut4, resulting in higher glucose uptake upon insulin stimuli. DNJ appeared to mitigate the insulin resistance‐driven increase in phosphorylated tau and Aβ1‐42 levels by promoting insulin‐induced phosphorylation of GSK3B (a major tau kinase) and enhancing mRNA expression of the insulin‐degrading enzyme (IDE) pivotal for insulin and Aβ clearance. Overall, our study unveils probable mechanisms underlying the potential benefits of DNJ for AD, wherein DNJ attenuates tau and amyloid pathologies by reversing neuronal insulin resistance. This provides a scientific basis for expanding the use of DNJ‐containing products for neuroprotective purposes and prompts further research into compounds with similar mechanisms of action.

  3. Sheep-derived cell immortalization through the expression of cell cycle regulators and biological characterization using transcriptomes. International-journal

    Noe Kikuchi, Himari Matsusaka, Lanlan Bai, Hiroaki Sano, Takahiro Eitsuka, Kiyotaka Nakagawa, Eriko Sugano, Taku Ozaki, Hiroshi Tomita, Tohru Kiyono, Tomokazu Fukuda

    Cell biology international　47　(9)　1491-1501　2023/09

    DOI： 10.1002/cbin.12034 　

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    Sheep are important domestic animals for the production of wool and meat. Although numerous cultured cell lines from humans and mice have been established, the number of cell lines derived from sheep is limited. To overcome this issue, the efficient establishment of a sheep-derived cell line and its biological characterization is reported. Mutant cyclin-dependent kinase 4, cyclin D1, and telomerase reverse transcriptase were introduced into sheep muscle-derived cells in an attempt to immortalize primary cells using the K4DT method. Furthermore, the SV40 large T oncogene was introduced into the cells. The successful immortalization of sheep muscle-derived fibroblasts was shown using the K4DT method or SV40 large T antigen. Furthermore, the expression profile of established cells showed close biological characteristics of ear-derived fibroblasts. This study provides a useful cellular resource for veterinary medicine and cell biology.

  4. The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin. International-journal

    Takashi Nakatomi, Mayuko Itaya-Takahashi, Yosuke Horikoshi, Naoki Shimizu, Isabella Supardi Parida, Mirinthorn Jutanom, Takahiro Eitsuka, Yoshikazu Tanaka, Jean-Marc Zingg, Tatsuya Matsura, Kiyotaka Nakagawa

    Scientific reports　13　(1)　7392-7392　2023/05/06

    DOI： 10.1038/s41598-023-34584-z 　

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    Vitamin E is classified into tocopherol (Toc) and tocotrienol (T3) based on its side chains. T3 generally has higher cellular uptake than Toc, though the responsible mechanism remains unclear. To elucidate this mechanism, we hypothesized and investigated whether serum albumin is a factor that induces such a difference in the cellular uptake of Toc and T3. Adding bovine serum albumin (BSA) to serum-depleted media increased the cellular uptake of T3 and decreased that of Toc, with varying degrees among α-, β-, γ-, and δ-analogs. Such enhanced uptake of α-T3 was not observed when cells were incubated under low temperature (the uptake of α-Toc was also reduced), suggesting that Toc and T3 bind to albumin to form a complex that results in differential cellular uptake of vitamin E. Fluorescence quenching study confirmed that vitamin E certainly bound to BSA, and that T3 showed a higher affinity than Toc. Molecular docking further indicated that the differential binding energy of Toc or T3 to BSA is due to the Van der Waals interactions via their side chain. Overall, these results suggested that the affinity of Toc and T3 to albumin differs due to their side chains, causing the difference in their albumin-mediated cellular uptake. Our results give a better mechanistic insight into the physiological action of vitamin E.

  5. Immortalization of primary cells derived from the endangered Ryukyu long-furred rat

    Lanlan Bai, Noe Kikuchi, Takahiro Eitsuka, Himari Matsusaka, Kiyotaka Nakagawa, Masafumi Katayama, Keiko Ito, Miho Inoue-Murayama, Tohru Kiyono, Tomkazu Fukuda

    In Vitro Cellular & Developmental Biology - Animal　2023/03/27

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s11626-023-00757-3 　

    ISSN： 1071-2690

    eISSN： 1543-706X

  6. Correction to: Immortalization of primary cells derived from the endangered Ryukyu long-furred rat (In Vitro Cellular & Developmental Biology - Animal, (2023), 59, 3, (224-233), 10.1007/s11626-023-00757-3)

    Lanlan Bai, Noe Kikuchi, Takahiro Eitsuka, Himari Matsusaka, Kiyotaka Nakagawa, Masafumi Katayama, Keiko Ito, Miho Inoue-Murayama, Tohru Kiyono, Tomokazu Fukuda

    In Vitro Cellular and Developmental Biology - Animal　59　(3)　234　2023/03

    DOI： 10.1007/s11626-023-00767-1 　

    ISSN： 1071-2690

    eISSN： 1543-706X

  7. Immortalization of common marmoset-derived fibroblasts via expression of cell cycle regulators using the piggyBac transposon. International-journal

    Ai Orimoto, Haruka Shinohara, Takahiro Eitsuka, Kiyotaka Nakagawa, Erika Sasaki, Tohru Kiyono, Tomokazu Fukuda

    Tissue & cell　77　101848-101848　2022/06/02

    DOI： 10.1016/j.tice.2022.101848 　

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    Common marmosets are non-human primate models used in biomedical research and genome editing technology. This study aimed to establish cell lines from common marmosets and evaluate their characteristics. We obtained normal fibroblasts derived from muscle tissues of two common marmosets and immortalized them with the introduction of a mutat form of cyclin-dependent kinase 4 (CDK4R24C), Cyclin D1, and telomere reverse transcriptase (TERT) using the piggyBac transposon. Compared to parental cells, the immortalized cell lines (named K4DT cells) showed telomerase activity and an accelerated cell proliferation rate. To our knowledge, this is the first study describing the successful establishment of immortalized common marmoset-derived fibroblasts using piggyBac transposition of CDK4R24C, Cyclin D1, and TERT. Our generated cell lines might be a beneficial tool for future studies on disease modeling and targeted gene therapies.

  8. Ethanolamine Plasmalogen Suppresses Apoptosis in Human Intestinal Tract Cells in Vitro by Attenuating Induced Inflammatory Stress. International-journal Peer-reviewed

    Ephantus Nguma, Shinji Yamashita, Kei Kumagai, Yurika Otoki, Ayaka Yamamoto, Takahiro Eitsuka, Kiyotaka Nakagawa, Teruo Miyazawa, Mikio Kinoshita

    ACS omega　6　(4)　3140-3148　2021/02/02

    DOI： 10.1021/acsomega.0c05545 　

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    Ethanolamine plasmalogen (PlsEtn) is a subtype of ethanolamine glycerophospholipids (EtnGpl). Recently, PlsEtn has attracted increasing research interest due to its beneficial effects in health and disease; however, its functional role in colonic health has not been well established. This study was conducted to determine the mechanism underlying the antiapoptotic effect of PlsEtn in human intestinal tract cells under induced inflammatory stress. Lipopolysaccharide induced apoptosis of differentiated Caco-2 cells, which was suppressed by EtnGpl in a dose-dependent manner. Cells treated with ascidian muscle EtnGpl containing high levels of PlsEtn demonstrated a lower degree of apoptosis, and downregulated TNF-α and apoptosis-related proteins compared to those treated with porcine liver EtnGpl containing low PlsEtn. This indicates that PlsEtn exerted the observed effects, which provided protection against induced inflammatory stress. Overall, our results suggest that PlsEtn with abundant vinyl ether linkages is potentially beneficial in preventing the initiation of inflammatory bowel disease and colon cancer.

  9. Structural changes of ethanolamine plasmalogen during intestinal absorption. International-journal Peer-reviewed

    Takumi Takahashi, Reina Kamiyoshihara, Yurika Otoki, Junya Ito, Shunji Kato, Takuji Suzuki, Shinji Yamashita, Takahiro Eitsuka, Ikuo Ikeda, Kiyotaka Nakagawa

    Food & function　11　(9)　8068-8076　2020/09/23

    DOI： 10.1039/d0fo01666g 　

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    Considerable attention has been paid to the absorption mechanisms of plasmalogen (Pls) because its intake has been expected to have preventive effects on brain-related diseases. Possible structural changes of Pls during absorption (i.e., preferential arachidonic acid re-esterification at the sn-2 position and base conversion of ethanolamine Pls (PE-Pls) into choline Pls (PC-Pls)) have previously been proposed. Since the physiological functions of Pls differ according to its structure, further elucidation of such structural changes during absorption is important to understand how Pls exerts its physiological effects in vivo. Hence, the absorption mechanism of Pls was investigated using the lymph-cannulation method and the everted jejunal sac model, with a focus on Pls molecular species. In the lymph-cannulation method, relatively high amounts of PE-Pls 18:0/20:4 and PC-Pls 18:0/20:4 were detected from the lymph even though these species were minor in the administered emulsion. Moreover, a significant increase of PE-Pls 18:0/20:4 and PC-Pls 18:0/20:4 in the intestinal mucosa was also confirmed by the everted jejunal sac model. Therefore, structural changes of PE-Pls in the intestinal mucosa were strongly suggested. The results of this study may provide an understanding of the relationship between intestinal absorption of Pls and exertion of its physiological functions in vivo.

  10. Intestinal Absorption and Tissue Distribution of Aza-Sugars from Mulberry Leaves and Evaluation of Their Transport by Sugar Transporters. International-journal Peer-reviewed

    Soo Takasu, Isabella S Parida, Junya Ito, Yoshihiro Kojima, Takahiro Eitsuka, Toshiyuki Kimura, Kiyotaka Nakagawa

    Journal of agricultural and food chemistry　68　(24)　6656-6663　2020/06/17

    DOI： 10.1021/acs.jafc.0c03005 　

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    Mulberry leaves are rich in aza-sugars, particularly 1-deoxynojirimycin (DNJ), fagomine, and 2-O-α-d-galactopyranosyl-1-deoxynojirimycin (GAL-DNJ), which have antidiabetes and antiobesity properties. To help us understand the mechanisms of action of aza-sugars, pharmacokinetic studies are necessary. Therefore, in this study, we evaluated and compared the absorption and organ distribution of these aza-sugars in rats. Following oral intake, DNJ exhibited the highest plasma concentration followed by fagomine and GAL-DNJ. Meanwhile, similar amounts of DNJ and fagomine were present in organs, while GAL-DNJ was hardly detected, suggesting the diversity in absorption and distribution characteristics of these aza-sugars. We then investigated the role of the sodium-glucose cotransporter and the glucose transporter (GLUT) in the transport of aza-sugars and found that both are involved in DNJ transport, while transport of fagomine is solely facilitated by the GLUT. These findings provide insight into the bioavailability and bioactive mechanisms of these aza-sugars.

  11. Primary and immortalized cell lines derived from the Amami rabbit (Pentalagus furnessi) and evolutionally conserved cell cycle control with CDK4 and Cyclin D1. International-journal Peer-reviewed

    Ai Orimoto, Masafumi Katayama, Tetsuya Tani, Keiko Ito, Takahiro Eitsuka, Kiyotaka Nakagawa, Miho Inoue-Murayama, Manabu Onuma, Tohru Kiyono, Tomokazu Fukuda

    Biochemical and biophysical research communications　525　(4)　1046-1053　2020/05/14

    DOI： 10.1016/j.bbrc.2020.03.036 　

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    The Amami rabbit (Pentagulus furnessi) is a dark brown-furred rabbit classified as an endangered species and only found in the Amami Islands of Japan. They are often called living fossils because they retain primitive characteristics of ancient rabbits that lived approximately 1 million years ago, such as short feet and hind legs and small ears. Although the ancient rabbit has disappeared due to the competition with European rabbit (Oryctolagus cuniculus) in the most of the Asian area, Amami rabbit survived since Amami Islands has isolated from Japan and Taiwan. Although Amari rabbit is one of the protected animals, their population decreases each year due to human activities, such as deforestation and roadkill. In this study, we collected roadkill samples of Amami rabbits and established primary and immortalized fibroblast cell lines. Combined expression of human-derived mutant Cyclin-dependent kinase 4, Cyclin D1, and hTERT allowed us to immortalize fibroblasts successfully in three individuals of Amami rabbits. The immortalized fibroblasts dramatically extended the cell culture period, when it was compared with the cell culture period of wild type cells. Furthermore, the immortalized cells maintained their normal chromosomal pattern (2n = 46). Our results suggest that cellular senescence which mainly regulated by p16-RB signaling pathway is conserved in animal evolution at least from 1 million years ago.

  12. Supplementation of Bacillus amyloliquefaciens AS385 culture broth powder containing 1-deoxynojirimycin in a high-fat diet altered the gene expressions related to lipid metabolism and insulin signaling in mice epididymal white adipose tissue. International-journal Peer-reviewed

    Isabella Supardi Parida, Soo Takasu, Junya Ito, Ryoichi Ikeda, Kenji Yamagishi, Toshiyuki Kimura, Takahiro Eitsuka, Kiyotaka Nakagawa

    Food & function　11　(5)　3926-3940　2020/05/01

    DOI： 10.1039/d0fo00271b 　

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    1-Deoxynojirimycin (DNJ) has been known for its functional properties, such as its anti-hyperglycemic and anti-obesity activities. Previously, we developed a sustainable procedure to produce culture broth powder (CBP) containing DNJ using Bacillus amyloliquefaciens AS385 and demonstrated its regulatory effect on the blood glucose and lipid parameters in C57BL/6J mice. The present study was aimed to determine the molecular mechanism underlying the physiological effects of CBP intake in different concentrations (low, medium and high) towards the development of high-fat diet (HFD)-induced metabolic disorders. Ten-week consumption of CBP-supplemented diets ameliorated HFD-induced adiposity, glucose intolerance, and reduced insulin sensitivity in C57BL/6J mice. To investigate how these physiological events could take place, we analyzed the expression of genes involved in lipid metabolism and insulin signaling in epididymal white adipose tissue and found that CBP had a regulatory effect on the expression of genes related to lipid metabolism (Pparγ, Srebf1c, Acc, Scd, Hsl, Lpl), adiponectin secretion (Foxo1 and Sirt1), and insulin signaling (Irs1 and Akt2). Next, we confirmed that DNJ acted as the main active component in CBP and detected the dose-dependent DNJ uptake in vital metabolic tissues, which may explain the dose-dependent alteration in the metabolic parameters and related gene expressions following the CBP intake in this study. Collectively, our results suggested that DNJ intake in the form of CBP prevented the progression of HFD-induced metabolic disorders through regulation of adipocyte gene expression involved in lipid metabolism and insulin signaling.

  13. Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor International-journal Peer-reviewed

    Tomokazu Fukuda, Kouhei Takahashi, Shin Takase, Ai Orimoto, Takahiro Eitsuka, Kiyotaka Nakagawa, Tohru Kiyono

    Frontiers in Cell and Developmental Biology　8　157-157　2020/03/18

    Publisher: Frontiers Media SA

    DOI： 10.3389/fcell.2020.00157 　

    eISSN： 2296-634X

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    Androgenetic alopecia (AGA) is the most common type of hair loss, and is mainly caused by the biological effects of testosterone on dermal papilla cells (DPCs). In vitro culturing of DPCs might be a useful tool for the screening of target molecule of AGA. However, primary DPCs cannot continuously proliferate owing to cellular senescence and cell culture stress. In this study, we introduced mutant cyclin-dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT) into DPCs. We confirmed protein expression of CDK4 and Cyclin D1, and enzymatic activity of TERT. Furthermore, we found the established cell line was free from cellular senescence. We also introduced the androgen receptor gene using a recombinant retrovirus, to compensate the transcriptional suppressed endogenous androgen receptor in the process of cell proliferation. Furthermore, we detected the efficient nuclear translocation of androgen receptor into the nucleus after the treatment of dihydrotestosterone, indicating the functionality of our introduced receptor. Our established cell line is a useful tool to identify the downstream signaling pathway, which activated by the testosterone.

  14. Analysis of oxidation products of α-tocopherol in extra virgin olive oil using liquid chromatography-tandem mass spectrometry. International-journal Peer-reviewed

    Rena Tanno, Shunji Kato, Naoki Shimizu, Junya Ito, Shuntaro Sato, Yusuke Ogura, Masayoshi Sakaino, Takashi Sano, Takahiro Eitsuka, Shigefumi Kuwahara, Teruo Miyazawa, Kiyotaka Nakagawa

    Food chemistry　306　125582-125582　2020/02/15

    DOI： 10.1016/j.foodchem.2019.125582 　

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    When α-tocopherol (α-Toc) exerts its antioxidative effect, a portion of α-Toc is converted to certain oxidation products. Although accumulation of such oxidation products is considered to cause a deterioration in the quality of foods, their distribution and generation in food samples have been still unknown. In this study, we tried to analyze α-Toc hydroperoxide (Toc-OOH) stereoisomers and tocopherylquinone (TQ) in extra virgin olive oil (EVOO) using liquid chromatography-tandem mass spectrometry. Photo-irradiation (5000 lx) to EVOO increased Toc-OOH stereoisomers but not TQ. In contrast, thermal oxidation (150 °C) of EVOO increased TQ but not Toc-OOH. We considered that the generation of Toc-OOH and TQ were due to the [4+2]-cycloaddition reaction and proton donation from the phenolic hydrogen, respectively. Our data and method would be helpful for understanding of α-Toc oxidation mechanisms in edible oil samples or the estimation of food quality.

  15. Author Correction: Definitive evidence of the presence of 24-methylenecycloartanyl ferulate and 24-methylenecycloartanyl caffeate in barley. International-journal Peer-reviewed

    Junya Ito, Kazue Sawada, Yusuke Ogura, Fan Xinyi, Halida Rahmania, Tomoyo Mohri, Noriko Kohyama, Eunsang Kwon, Takahiro Eitsuka, Hiroyuki Hashimoto, Shigefumi Kuwahara, Teruo Miyazawa, Kiyotaka Nakagawa

    Scientific reports　10　(1)　974-974　2020/01/17

    DOI： 10.1038/s41598-020-57690-8 　

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    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

  16. Correction: Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon). International-journal Peer-reviewed

    Tetsuya Tani, Takahiro Eitsuka, Masafumi Katayama, Takashi Nagamine, Yumiko Nakaya, Hajime Suzuki, Tohru Kiyono, Kiyotaka Nakagawa, Miho Inoue-Murayama, Manabu Onuma, Tomokazu Fukuda

    PloS one　15　(5)　e0234054　2020

    DOI： 10.1371/journal.pone.0234054 　

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    [This corrects the article DOI: 10.1371/journal.pone.0221364.].

  17. Efficient immortalization of human dental pulp stem cells with expression of cell cycle regulators with the intact chromosomal condition. International-journal Peer-reviewed

    Ai Orimoto, Seiko Kyakumoto, Takahiro Eitsuka, Kiyotaka Nakagawa, Tohru Kiyono, Tomokazu Fukuda

    PloS one　15　(3)　e0229996　2020

    DOI： 10.1371/journal.pone.0229996 　

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    Clinical studies have recently demonstrated that autologous transplantation of mobilized dental pulp stem cells is a safe and efficacious potential therapy for pulp regeneration. However, some limitations need to be addressed, such as the high cost of the safety and quality control tests for isolated individual dental pulp cell products before transplantation. Therefore, more efficient in vitro culturing of human dental pulp stem cells might be useful for providing low cost and high reliability testing for pulp regeneration therapy. In this study, we established a novel immortalized dental pulp stem cell line by co-expressing a mutant cyclin-dependent kinase 4 (CDK4R24C), Cyclin D1, and telomerase reverse transcriptase (TERT). The established cell line maintained its original diploid chromosomes and stemness characteristics and exhibited an enhanced proliferation rate. In addition, we showed the immortalized human dental pulp stem cells still keeps their osteogenic and adipogenic differentiation abilities under appropriate culture conditions even though the cell proliferation was accelerated. Taken together, our established cell lines could serve as a useful in vitro tool for pulp regeneration therapy, and can contribute to reproducibility and ease of cell handling, thereby saving time and costs associated with safety and quality control tests.

  18. Evaluation of squalene oxidation mechanisms in human skin surface lipids and shark liver oil supplements. International-journal Peer-reviewed

    Naoki Shimizu, Junya Ito, Shunji Kato, Takahiro Eitsuka, Tetsuo Saito, Hiroshi Nishida, Teruo Miyazawa, Kiyotaka Nakagawa

    Annals of the New York Academy of Sciences　1457　(1)　158-165　2019/12

    DOI： 10.1111/nyas.14219 　

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    Squalene is a terpenoid found in human skin surface lipids (SSLs) and foods that possesses beneficial properties. However, since oxidation of squalene causes various complications, it is necessary to identify the mechanisms by which squalene is oxidized. In this study, we aimed to determine the oxidation mechanisms of squalene in SSLs and shark liver oil (SLO) supplements by the analysis of squalene monohydroperoxide (SQOOH) isomers, on the basis of our previous finding that different oxidation mechanisms yield different SQOOH isomers. Liquid chromatography-tandem mass spectrometry analysis of SQOOH isomers revealed that squalene in human SSLs was oxidized by singlet oxygen oxidation, whereas squalene in SLO was oxidized mainly by free radicals. As a result, we have presented the first evidence suggesting that the analysis of SQOOH isomers enables estimation of oxidation mechanisms. Estimating oxidation mechanisms by analyzing SQOOH isomers may provide a foundation for the prevention of skin diseases and food deterioration via regulation of squalene oxidation.

  19. Definitive evidence of the presence of 24-methylenecycloartanyl ferulate and 24-methylenecycloartanyl caffeate in barley. International-journal Peer-reviewed

    Junya Ito, Kazue Sawada, Yusuke Ogura, Fan Xinyi, Halida Rahmania, Tomoyo Mohri, Noriko Kohyama, Eunsang Kwon, Takahiro Eitsuka, Hiroyuki Hashimoto, Shigefumi Kuwahara, Teruo Miyazawa, Kiyotaka Nakagawa

    Scientific reports　9　(1)　12572-12572　2019/08/29

    DOI： 10.1038/s41598-019-48985-6 　

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    γ-Oryzanol (OZ), which has a lot of beneficial effects, is a mixture of ferulic acid esters of triterpene alcohols (i.e., triterpene alcohol type of OZ (TTA-OZ)) and ferulic acid esters of plant sterols (i.e., plant sterol type of OZ (PS-OZ)). Although it has been reported that OZ is found in several kinds of cereal typified by rice, TTA-OZ (e.g., 24-methylenecycloartanyl ferulate (24MCA-FA)) has been believed to be characteristic to rice and has not been found in other cereals. In this study, we isolated a compound considered as a TTA-OZ (i.e., 24MCA-FA) from barley and determined the chemical structure using by HPLC-UV-MS, high-resolution MS, and NMR. Based on these results, we proved for the first time that barley certainly contains 24MCA-FA (i.e., TTA-OZ). During the isolation and purification of 24MCA-FA from barley, we found the prospect that a compound with similar properties to OZ (compound-X) might exist. To confirm this finding, the compound-X was also isolated, determined the chemical structure, and identified as a caffeic acid ester of 24-methylenecycloartanol (24MCA-CA), which has rarely been reported before. We also quantified these compounds in various species of barley cultivars and found for the first time the existence of 24MCA-FA and 24MCA-CA in various barley. Through these findings, it opens the possibility to use barley as a new source of 24MCA-FA and 24MCA-CA.

  20. The differential cellular uptake of curcuminoids in vitro depends dominantly on albumin interaction. International-journal Peer-reviewed

    Mayuko Itaya, Taiki Miyazawa, Jean-Marc Zingg, Takahiro Eitsuka, Angelo Azzi, Mohsen Meydani, Teruo Miyazawa, Kiyotaka Nakagawa

    Phytomedicine : international journal of phytotherapy and phytopharmacology　59　152902-152902　2019/06

    DOI： 10.1016/j.phymed.2019.152902 　

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    BACKGROUND: Curcuminoids, mainly present in the plant rhizomes of turmeric (Curcuma longa), consist of mainly three forms (curcumin (CUR), bisdemethoxycurcumin (BDMC) and demethoxycurcumin (DMC)). It has been reported that different forms of curcuminoids possess different biological activities. However, the mechanisms associated with these differences are not well-understood. Recently, our laboratory found differences in the cellular uptake of these curcuminoids. Therefore, it has been inferred that these differences contribute to the different biological activities. PURPOSE: In this study, we investigated the mechanisms of differential cellular uptake of these curcuminoids. METHOD: Based on our previous study, we hypothesized the differential cellular uptake is caused by (I) polarity, (II) transporters, (III) metabolism rate of curcuminoids and (IV) medium components. These four hypotheses were each investigated by (I) neutralizing the polarities of curcuminoids by encapsulation into poly(lactic-co-glycolic) acid nanoparticles (PLGA-NPs), (II) inhibition of polyphenol-related absorption transporters, (III) analysis of the cellular curcuminoids and their metabolites by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and (IV) use of different mediums in cell study. RESULTS: The differential cellular uptake was not affected by (I-III). However, when investigating (IV), not only CUR but also BDMC and DMC were incorporated into cells when serum free media was used. Furthermore, when we used the serum free medium containing bovine serum albumin (BSA), only CUR was taken up but BDMC and DMC were not. Therefore, we identified that the differential cellular uptake of curcuminoids is caused by the medium components, especially BSA. Also, the fluorescence quenching study suggested that differential cellular uptake is due to the different interaction between BSA and each curcuminoid. CONCLUSION: The differential cellular uptake of curcuminoids was caused by the different interaction between curcuminoids and BSA. The results from this study might give clues on the mechanisms by which curcuminoids exhibit different physiological activities.

  21. Comparison of Blood Profiles of γ-Oryzanol and Ferulic Acid in Rats after Oral Intake of γ-Oryzanol. International-journal Peer-reviewed

    Takumi Kokumai, Junya Ito, Eri Kobayashi, Naoki Shimizu, Hiroyuki Hashimoto, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    Nutrients　11　(5)　2019/05/25

    DOI： 10.3390/nu11051174 　

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    γ-Oryzanol (OZ), a bioactive phytochemical abundant in cereals such as rice, has been reported to be mainly hydrolyzed to ferulic acid (FA) in the body. Meanwhile, in our previous study, we revealed that a part of OZ is absorbed into the body and exists in its intact form. However, the comprehensive absorption profile of OZ and its metabolites (e.g., FA) after OZ intake has not been fully elucidated yet. Therefore, in this study, we measured the concentrations of OZ, FA, and FA conjugates (i.e., FA sulfate and glucuronide) in the blood of rats with the use of HPLC-MS/MS after a single oral administration of 300 µmol/kg body weight of rice bran OZ (RBOZ). As a result, intact OZ along with FA and FA conjugates existed in the blood, which implied that these constituents may all contribute to the physiological effects under OZ intake. Additionally, when an equimolar amount of FA (300 µmol/kg body weight) was administered, it was found that the absorption profile of FA was significantly different from that when RBOZ was administered.

  22. Expression of mutant Cyclin-dependent-kinase, CyclinD, and TERT extends the proliferation period until senescence in chicken and Okinawa rail derived fibroblasts. Peer-reviewed

    Katayama M, Kiyono T, Ohmaki H, Eitsuka T, Endo D, Inoue-Murayama M, Nakajima N, Onuma M, Fukuda T

    Journal of Cellular Physiology　2019/05

  23. Extended proliferation of chicken- and Okinawa rail-derived fibroblasts by expression of cell cycle regulators. International-journal Peer-reviewed

    Masafumi Katayama, Tohru Kiyono, Hitomi Ohmaki, Takahiro Eitsuka, Daiji Endoh, Miho Inoue-Murayama, Nobuyoshi Nakajima, Manabu Onuma, Tomokazu Fukuda

    Journal of cellular physiology　234　(5)　6709-6720　2019/05

    DOI： 10.1002/jcp.27417 　

    ISSN： 0021-9541

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    Although immortalized cultured cells are useful for various functional assays or transcriptome analysis, highly efficient and reproducible immortalization methods have not been developed in avian-derived cells. We introduced the simian virus 40 T antigen (SV40T) and human papillomavirus (HPV)-E6E7 to chick and Okinawa rail (endangered species)derived fibroblast. As a result, neither the SV40T nor E6E7 genes could induce avian cell immortality. Accordingly, we attempted to use a recently developed immortalization method, which involved the coexpression of mutant cyclin-dependent kinase 4 (CDK4), Cyclin D, and TERT (K4DT method) in these avian cells. Although the K4DT method could not efficiently induce the efficient immortalization in mass cell population, cellular division until the senescence was significantly extended by K4DT, we succeeded to obtain the immortalized avian cells (chick K4DT: one clone, Okinawa rail K4DT: three clones, Okinawa rail K4DT + telomerase RNA component: one clone) with K4DT expression. We conclude that K4DT expression is used to extend the cell division and immortalization of avian-derived cells.

  24. Evaluation of γ-oryzanol Accumulation and Lipid Metabolism in the Body of Mice Following Long-Term Administration of γ-oryzanol. International-journal Peer-reviewed

    Eri Kobayashi, Junya Ito, Naoki Shimizu, Takumi Kokumai, Shunji Kato, Kazue Sawada, Hiroyuki Hashimoto, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    Nutrients　11　(1)　2019/01/06

    DOI： 10.3390/nu11010104 　

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    γ-Oryzanol (OZ), abundant in rice bran oil, has gained attention due to its physiological activities (e.g., lipid-lowering effects). However, the absorption and metabolism of orally ingested OZ have not yet been fully elucidated. In this study, diets containing normal or high contents of OZ were fed to obesity model mice for 8 weeks, and OZ concentrations in plasma and organs were analyzed by HPLC-MS/MS. To evaluate the relationship between OZ accumulation and lipid metabolism in vivo, lipid concentrations in the mice plasma and liver were also measured. As a result, the accumulation of intact OZ in plasma and organs was seen in mice fed diets containing OZ, where mice fed diets containing higher OZ contents demonstrated higher levels of OZ accumulation and lower amounts of plasma lipids. These results, in combination with our additional data from a single oral administration test, suggest the possibility that intact OZ, along with its metabolites (e.g., ferulic acid), is biologically-active.

  25. Physiological Effects and Organ Distribution of Bacillus amyloliquefaciens AS385 Culture Broth Powder Containing 1-Deoxynojirimycin in C57BL/6J Mice. Peer-reviewed

    Isabella Supardi Parida, Soo Takasu, Junya Ito, Ryoichi Ikeda, Kenji Yamagishi, Toshiyuki Kimura, Teruo Miyazawa, Takahiro Eitsuka, Kiyotaka Nakagawa

    Journal of nutritional science and vitaminology　65　(2)　157-163　2019

    DOI： 10.3177/jnsv.65.157 　

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    1-Deoxynojirimycin (DNJ) has been known as a potent α-glucosidase inhibitor from mulberry leaves and considered beneficial in prevention of type 2 diabetes. Due to limited amount of DNJ in mulberry leaves, recent studies have focused in finding alternative source that can produce higher amount of DNJ. Previously, we produced a high DNJ-containing culture medium from Bacillus amyloliquefaciens AS385 and constructed a concentration method of bacterial culture medium using cation exchange column. However, less complicated concentration procedure is necessary to save time and cost during the large-scale production. Therefore, we developed a simpler concentration method using anion exchange resin to yield B. amyloliquefaciens AS385 culture broth powder (CBP; 1% DNJ) and evaluated the physiological effects of 5-wk dietary CBP intake in C57BL/6J mice. CBP intake tended to suppress the elevation of blood glucose level during oral glucose tolerance test. Moreover, CBP intake significantly lowered the fasting plasma glucose level and white adipose tissue mass. Next, we evaluated the absorption and distribution of DNJ in mice organs after daily CBP intake. We found detectable amount of DNJ in organs with intestine and kidney as the major targeted organs. We concluded that the DNJ content in CBP is absorbed from digestive tract, distributed and accumulated in organs, which most likely to contribute to the alteration of blood glucose regulation and adiposity in C57BL/6J mice. Our study was the first to report the physiological effects of CBP produced from B. amyloliquefaciens AS385 and the organ distribution of DNJ from CBP.

  26. The Adjuvant Effect of Squalene, an Active Ingredient of Functional Foods, on Doxorubicin-Treated Allograft Mice. International-journal Peer-reviewed

    Hari Narayan Bhilwade, Naoto Tatewaki, Tetsuya Konishi, Miyako Nishida, Takahiro Eitsuka, Hironobu Yasui, Osamu Inanami, Osamu Handa, Yuji Naito, Nobuo Ikekawa, Hiroshi Nishida

    Nutrition and cancer　71　(7)　1153-1164　2019

    DOI： 10.1080/01635581.2019.1597900 　

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    Many functional foods or physiologically active ingredients derived from plants and animals are actively being investigated for their role in chronic disease prevention. Squalene (SQ) is found as active ingredient in the functional foods predominantly present in olive oil and shark liver oil. It is known that during chemotherapy anticancer drugs induce inflammation. SQ has been thought to prevent and suppress inflammation; however, there is little direct evidence available. We examined the adjuvant effect of SQ on tumor-transplanted mice along with anticancer drug doxorubicin (DOX). SQ significantly suppressed the DOX-induced increase in prostaglandin E2 (PGE2) concentration (P < 0.05) in plasma of tumor-bearing mice. SQ inhibited the numbers of writhing response (P < 0.05), formalin-induced pain and decreased COX-2 and substance P expression in the tumor tissue compared to control mice and also enhanced the antitumor efficacy of DOX in allograft mice. Thus, SQ reduces inflammation through modulation of PGE2 production indicating its potential as an adjuvant during chemotherapy in tumor-bearing mice.

  27. Human-Derived Corneal Epithelial Cells Expressing Cell Cycle Regulators as a New Resource for in vitro Ocular Toxicity Testing. International-journal Peer-reviewed

    Tomokazu Fukuda, Ryo Gouko, Takahiro Eitsuka, Ryusei Suzuki, Kohei Takahashi, Kiyotaka Nakagawa, Eriko Sugano, Hiroshi Tomita, Tohru Kiyono

    Frontiers in genetics　10　587-587　2019

    DOI： 10.3389/fgene.2019.00587 　

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    The Draize test has been used on rabbits since the 1960s to evaluate the irritation caused by commercial chemicals in products such as cosmetics or hairdressing products. However, since 2003, such tests, including the Draize test for cosmetics, have been prohibited in European countries because they are considered problematic to animal welfare. For this reason, replacement of in vivo methods with the alternative in vitro methods has become an important goal. In this study, we established a corneal epithelial cell line co-expressing a mutant cyclin-dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT). The established cell line maintained its original morphology and had an enhanced proliferation rate. Furthermore, the cells showed a significant, dose-dependent decrease in viability in an irritation test using glycolic acid and Benzalkonium chloride. These cells can now be shared with toxicology scientists and should contribute to increasing the reproducibility of chemical testing in vitro.

  28. Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon). International-journal Peer-reviewed

    Tetsuya Tani, Takahiro Eitsuka, Masafumi Katayama, Takashi Nagamine, Yumiko Nakaya, Hajime Suzuki, Tohru Kiyono, Kiyotaka Nakagawa, Miho Inoue-Murayama, Manabu Onuma, Tomokazu Fukuda

    PloS one　14　(8)　e0221364　2019

    DOI： 10.1371/journal.pone.0221364 　

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    The Bonin flying fox (Pteropus pselaphon) is one of the most critically endangered species of animals. The number of this species is estimated to be around 150; being classified at the top rank in the list by International Union of Animal Conservation. Our group previously showed that expression of CDK4, CYCLIN D1, and telomerase reverse transcriptase (TERT) efficiently induce immortalization of human, bovine, swine, monkey, and buffalo-derived cells. In this manuscript, we successfully established the primary cells from Bonin flying fox. We introduced CDK4, CYCLIN D1, and TERT into the primary cells. The established cells showed efficient expression of introduced genes at the protein level. Furthermore, the established cells were free from senescence, indicating it reached to immortalization. Moreover, we showed that interspecies somatic cell nuclear transfer of Bonin flying fox derived cell into bovine embryo allowed the development of the embryo to 8 cell stages. Our established cell has the potential to contribute to species conservation.

  29. Significance of Squalene in Rice Bran Oil and Perspectives on Squalene Oxidation. Peer-reviewed

    Naoki Shimizu, Junya Ito, Shunji Kato, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    Journal of nutritional science and vitaminology　65　(Supplement)　S62-S66　2019

    DOI： 10.3177/jnsv.65.S62 　

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    As an intermediate metabolite during the biosynthesis of sterols, squalene is found ubiquitously in plants and animals. In rice, squalene is contained in rice bran, and consequently, squalene in rice bran oil has gained attention. Studies have shown that the intake of squalene from food sources demonstrate various physiological benefits such as the prevention of cancer and cardiovascular disease. Squalene is also known as an effective antioxidant in edible oils. However, due to its chemical structure, squalene is susceptible to oxidation, which may cause a decline in the nutraceutical and antioxidative effects of squalene in edible oils. Oxidative degradation of squalene also results in the formation of scission products (i.e., aldehydes and ketones) which may lead to off-flavor. Since the rate of squalene oxidation depends on the factors that induce its oxidation (i.e., light or heat), emphasis on oxidation mechanisms is necessary. It has been demonstrated in previous studies that the oxidation products formed by the singlet oxygen oxidation and free radical oxidation of squalene are different, and more recently, we demonstrated that different squalene monohydroperoxide isomers are formed by each oxidation mechanism. We herein discuss the significance of squalene in rice bran oil as well as the oxidative degradation of squalene in edible oils with focus on oxidation mechanisms.

  30. Efficient immortalization of cells derived from critically endangered Tsushima leopard cat (Prionailurus bengalensis euptilurus) with expression of mutant CDK4, Cyclin D1, and telomerase reverse transcriptase. International-journal Peer-reviewed

    Ryo Gouko, Manabu Onuma, Takahiro Eitsuka, Masafumi Katayama, Kouhei Takahashi, Kiyotaka Nakagawa, Miho Inoue-Murayama, Tohru Kiyono, Tomokazu Fukuda

    Cytotechnology　70　(6)　1619-1630　2018/12

    DOI： 10.1007/s10616-018-0254-0 　

    ISSN： 0920-9069

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    Tsushima leopard cat is the subspecies of Amur cats, and it is classified as the most highest class of critically endangered animals. Although the protection activity is highly recognized, the number of animals is decreasing due to the human activity and invasion of domestic cats and infectious disease. In this study, we succeeded primary culture of normal fibroblasts derived from the Tsushima leopard cat (Prionailurus bengalensis euptilurus). Furthermore, we introduced the human derived mutant Cyclin Dependent Kinase 4, Cyclin D1, and telomere reverse transcriptase. We showed that the expression of these three genes efficiently immortalized cells derived from Tsushima leopard cat. Furthermore, we showed that the chromosome pattern of the established cells is identical with the original one. These data indicate that our method of immortalization is useful to establish cell lines from critically endangered cats, which potentially contributes to the re-generation of critically endangered animals from cultured cell with reproductive technique, such as somatic cloning.

  31. Absorption and Metabolism of Luteolin in Rats and Humans in Relation to in Vitro Anti-inflammatory Effects. International-journal Peer-reviewed

    Natsumi Hayasaka, Naoki Shimizu, Toshikazu Komoda, Satoshi Mohri, Tojiro Tsushida, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    Journal of agricultural and food chemistry　66　(43)　11320-11329　2018/10/31

    DOI： 10.1021/acs.jafc.8b03273 　

    ISSN： 0021-8561

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    Luteolin is a flavonoid present in plants in the form of aglycone or glucosides. In this study, luteolin glucosides (i.e., luteolin-7- O-β-d-glucoside, luteolin-7- O-[2-(β-d-apiosyl)-β-d-glucoside], and luteolin-7- O-[2-(β-d-apiosyl)-6-malonyl-β-d-glucoside]) prepared from green pepper leaves as well as luteolin aglycone were orally administered to rats. Regardless of the administered luteolin form, luteolin glucuronides were mainly detected from plasma and organs. Subsequently, luteolin aglycone, the most absorbed form of luteolin in rats, was orally administered to humans. As a result, luteolin-3'- O-sulfate was mainly identified from plasma, suggesting that not only luteolin form but also animal species affect the absorption and metabolism of luteolin. When LPS-treated RAW264.7 cells were treated with luteolin glucuronides and luteolin sulfate (the characteristic metabolites identified from rats and humans, respectively), the different luteolin conjugates were metabolized in different ways, suggesting that such difference in metabolism results in their difference in anti-inflammatory effects.

  32. Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize fibroblasts derived from loggerhead sea turtle (Caretta caretta). International-journal Peer-reviewed

    Tomokazu Fukuda, Takahiro Eitsuka, Kenichiro Donai, Masanori Kurita, Tomomi Saito, Hitoshi Okamoto, Kodzue Kinoshita, Masafumi Katayama, Hiroshi Nitto, Takafumi Uchida, Manabu Onuma, Hideko Sone, Miho Inoue-Murayama, Tohru Kiyono

    Scientific reports　8　(1)　9229-9229　2018/06/20

    DOI： 10.1038/s41598-018-27271-x 　

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    Conservation of the genetic resources of endangered animals is crucial for future generations. The loggerhead sea turtle (Caretta caretta) is a critically endangered species, because of human hunting, hybridisation with other sea turtle species, and infectious diseases. In the present study, we established primary fibroblast cell lines from the loggerhead sea turtle, and showed its species specific chromosome number is 2n = 56, which is identical to that of the hawksbill and olive ridley sea turtles. We first showed that intensive hybridization among multiple sea turtle species caused due to the identical chromosome number, which allows existence of stable hybridization among the multiple sea turtle species. Expressions of human-derived mutant Cyclin-dependent kinase 4 (CDK4) and Cyclin D dramatically extended the cell culture period, when it was compared with the cell culture period of wild type cells. The recombinant fibroblast cell lines maintained the normal chromosome condition and morphology, indicating that, at the G1/S phase, the machinery to control the cellular proliferation is evolutionally conserved among various vertebrates. To our knowledge, this study is the first to demonstrate the functional conservation to overcome the negative feedback system to limit the turn over of the cell cycle between mammalian and reptiles. Our cell culture method will enable the sharing of cells from critically endangered animals as research materials.

  33. Oxidation of squalene by singlet oxygen and free radicals results in different compositions of squalene monohydroperoxide isomers. International-journal Peer-reviewed

    Naoki Shimizu, Junya Ito, Shunji Kato, Yurika Otoki, Masashi Goto, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    Scientific reports　8　(1)　9116-9116　2018/06/14

    DOI： 10.1038/s41598-018-27455-5 　

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    Oxidation of squalene (SQ) causes a decline in the nutritional value of SQ in foods, as well as an accumulation of SQ oxidation products in skin lipids which lead to adverse skin conditions. However, mechanistic insights as to how SQ is oxidized by different oxidation mechanisms have been limited, and thus effective measures towards the prevention of SQ oxidation have not been identified. In this study, we oxidized SQ by either singlet oxygen oxidation or free radical oxidation, and monitored the formation of the six SQ monohydroperoxide (SQOOH) isomers, the primary oxidation products of SQ, at the isomeric level. While singlet oxygen oxidation of SQ resulted in the formation of similar amounts of the six SQOOH isomers, free radical oxidation of SQ mainly formed two types of isomers, 2-OOH-SQ and 3-OOH-SQ. The addition of β-carotene during singlet oxygen oxidation, and the addition of α-tocopherol during free radical oxidation lead to a dose-dependent decrease in the formation of SQOOH isomers. Such results suggest that the analysis of SQOOH at the isomeric level allows for the determination of the cause of SQ oxidation in various samples, and provides a foothold for future studies concerning the prevention of SQ oxidation.

  34. Modulation of Telomerase Activity in Cancer Cells by Dietary Compounds: A Review. International-journal Peer-reviewed

    Takahiro Eitsuka, Kiyotaka Nakagawa, Shunji Kato, Junya Ito, Yurika Otoki, Soo Takasu, Naoki Shimizu, Takumi Takahashi, Teruo Miyazawa

    International journal of molecular sciences　19　(2)　2018/02/06

    DOI： 10.3390/ijms19020478 　

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    Telomerase is expressed in ~90% of human cancer cell lines and tumor specimens, whereas its enzymatic activity is not detectable in most human somatic cells, suggesting that telomerase represents a highly attractive target for selective cancer treatment. Accordingly, various classes of telomerase inhibitors have been screened and developed in recent years. We and other researchers have successfully found that some dietary compounds can modulate telomerase activity in cancer cells. Telomerase inhibitors derived from food are subdivided into two groups: one group directly blocks the enzymatic activity of telomerase (e.g., catechin and sulfoquinovosyldiacylglycerol), and the other downregulates the expression of human telomerase reverse transcriptase (hTERT), the catalytic subunit of human telomerase, via signal transduction pathways (e.g., retinoic acid and tocotrienol). In contrast, a few dietary components, including genistein and glycated lipid, induce cellular telomerase activity in several types of cancer cells, suggesting that they may be involved in tumor progression. This review summarizes the current knowledge about the effects of dietary factors on telomerase regulation in cancer cells and discusses their molecular mechanisms of action.

  35. Determination of triacylglycerol oxidation mechanisms in canola oil using liquid chromatography-tandem mass spectrometry. International-journal Peer-reviewed

    Shunji Kato, Naoki Shimizu, Yasuhiko Hanzawa, Yurika Otoki, Junya Ito, Fumiko Kimura, Susumu Takekoshi, Masayoshi Sakaino, Takashi Sano, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    NPJ science of food　2　1-1　2018

    DOI： 10.1038/s41538-017-0009-x 　

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    Triacylglycerol (TG), the main component of edible oil, is oxidized by thermal- or photo- oxidation to form TG hydroperoxide (TGOOH) as the primary oxidation product. Since TGOOH and its subsequent oxidation products cause not only the deterioration of oil quality but also various toxicities, preventing the oxidation of edible oils is essential. Therefore understanding oxidation mechanisms that cause the formation of TGOOH is necessary. Since isomeric information of lipid hydroperoxide provides insights about oil oxidation mechanisms, we focused on dioleoyl-(hydroperoxy octadecadienoyl)-TG (OO-HpODE-TG) isomers, which are the primary oxidation products of the most abundant TG molecular species (dioleoyl-linoleoyl-TG) in canola oil. To secure highly selective and sensitive analysis, authentic OO-HpODE-TG isomer references (i.e., hydroperoxide positional/geometrical isomers) were synthesized and analyzed with HPLC-MS/MS. With the use of the method, photo- or thermal- oxidized edible oils were analyzed. While dioleoyl-(10-hydroperoxy-8E,12Z-octadecadienoyl)-TG (OO-(10-HpODE)-TG) and dioleoyl-(12-hydroperoxy-9Z,13E-octadecadienoyl)-TG (OO-(12-HpODE)-TG) were characteristically detected in photo-oxidized oils, dioleoyl-(9-hydroperoxy-10E,12E-octadecadienoyl)-TG and dioleoyl-(13-hydroperoxy-9E,11E-octadecadienoyl)-TG were found to increase depending on temperature in thermal-oxidized oils. These results prove that our methods not only evaluate oil oxidation in levels that are unquantifiable with peroxide value, but also allows for the determination of oil oxidation mechanisms. From the analysis of marketed canola oils, photo-oxidized products (i.e., OO-(10-HpODE)-TG and OO-(12-HpODE)-TG) were characteristically accumulated compared to the oil analyzed immediately after production. The method described in this paper is valuable in the understanding of oil and food oxidation mechanisms, and may be applied to the development of preventive methods against food deterioration.

  36. Analysis of Lutein in Mugwort (Artemisia princeps Pamp.) Paste and Evaluation of Manufacturing Processes Peer-reviewed

    Marina Komuro, Naoki Shimizu, Ryo Onuma, Yurika Otoki, Junya Ito, Shunji Kato, Ohki Higuchi, Keiichi Sudo, Seiichiro Suzuki, Teruo Miyazawa, Takahiro Eitsuka, Kiyotaka Nakagawa

    JOURNAL OF OLEO SCIENCE　66　(11)　1257-1262　2017/11

    DOI： 10.5650/jos.ess17117 　

    ISSN： 1345-8957

    eISSN： 1347-3352

  37. High purity tocotrienols attenuate atherosclerotic lesion formation in apoE-KO mice Peer-reviewed

    Akira Shibata, Teiko Kobayashi, Akira Asai, Takahiro Eitsuka, Shinichi Oikawa, Teruo Miyazawa, Kiyotaka Nakagawa

    JOURNAL OF NUTRITIONAL BIOCHEMISTRY　48　44-50　2017/10

    DOI： 10.1016/j.jnutbio.2017.06.009 　

    ISSN： 0955-2863

    eISSN： 1873-4847

  38. Cover Image, Volume 118, Number 3, March 2017.

    Fukuda T, Tani T, Haraguchi S, Donai K, Nakajima N, Uenishi H, Eitsuka T, Miyagawa M, Song S, Onuma M, Hoshino Y, Sato E, Honda A

    Journal of cellular biochemistry　118　(3)　i　2017/03

    DOI： 10.1002/jcb.25855 　

    ISSN： 0730-2312

  39. Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes Peer-reviewed

    Tomokazu Fukuda, Tetsuya Tani, Seiki Haraguchi, Kenichiro Donai, Nobuyoshi Nakajima, Hirohide Uenishi, Takahiro Eitsuka, Makoto Miyagawa, Sanghoun Song, Manabu Onuma, Yumi Hoshino, Eimei Sato, Arata Honda

    JOURNAL OF CELLULAR BIOCHEMISTRY　118　(3)　537-553　2017/03

    DOI： 10.1002/jcb.25727 　

    ISSN： 0730-2312

    eISSN： 1097-4644

  40. Mass Spectrometric Discrimination of Squalene Monohydroperoxide Isomers Peer-reviewed

    Naoki Shimizu, Hannah Bersabe, Junya Ito, Shunji Kato, Ryo Towada, Takahiro Eitsuka, Shigefumi Kuwahara, Teruo Miyazawa, Kiyotaka Nakagawa

    JOURNAL OF OLEO SCIENCE　66　(3)　227-234　2017/03

    DOI： 10.5650/jos.ess16159 　

    ISSN： 1345-8957

    eISSN： 1347-3352

  41. A Combination of delta-Tocotrienol and Ferulic Acid Synergistically Inhibits Telomerase Activity in DLD-1 Human Colorectal Adenocarcinoma Cells Peer-reviewed

    Takahiro Eitsuka, Naoto Tatewaki, Hiroshi Nishida, Kiyotaka Nakagawa, Teruo Miyazawa

    JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY　62　(5)　281-287　2016/10

    DOI： 10.3177/jnsv.62.281 　

    ISSN： 0301-4800

    eISSN： 1881-7742

  42. Synergistic Anticancer Effect of Tocotrienol Combined with Chemotherapeutic Agents or Dietary Components: A Review Invited Peer-reviewed

    Takahiro Eitsuka, Naoto Tatewaki, Hiroshi Nishida, Kiyotaka Nakagawa, Teruo Miyazawa

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES　17　(10)　2016/10

    DOI： 10.3390/ijms17101605 　

    ISSN： 1422-0067

  43. The fruit of Acanthopanax senticosus (Rupr. et Maxim.) Harms improves insulin resistance and hepatic lipid accumulation by modulation of liver adenosine monophosphate-activated protein kinase activity and lipogenic gene expression in high-fat diet-fed obese mice Peer-reviewed

    Tetsuo Saito, Miyako Nishida, Masafumi Saito, Akari Tanabe, Takahiro Eitsuka, Shi-Hua Yuan, Nobuo Ikekawa, Hiroshi Nishida

    NUTRITION RESEARCH　36　(10)　1090-1097　2016/10

    DOI： 10.1016/j.nutres.2016.09.004 　

    ISSN： 0271-5317

  44. Cellular conservation of endangered midget buffalo (Lowland Anoa, Bubalus quarlesi) by establishment of primary cultured cell, and its immortalization with expression of cell cycle regulators Peer-reviewed

    Tomokazu Fukuda, Yuuka Iino, Takahiro Eitsuka, Manabu Onuma, Masafumi Katayama, Koichi Murata, Miho Inoue-Murayama, Kumiko Hara, Emiko Isogai, Tohru Kiyono

    CYTOTECHNOLOGY　68　(5)　1937-1947　2016/10

    DOI： 10.1007/s10616-016-0004-0 　

    ISSN： 0920-9069

    eISSN： 1573-0778

  45. Squalene Inhibits ATM-Dependent Signaling in gamma IR-Induced DNA Damage Response through Induction of Wip1 Phosphatase Peer-reviewed

    Naoto Tatewaki, Tetsuya Konishi, Yuki Nakajima, Miyako Nishida, Masafumi Saito, Takahiro Eitsuka, Toshiyuki Sakamaki, Nobuo Ikekawa, Hiroshi Nishida

    PLOS ONE　11　(1)　e0147570-e0147570　2016/01

    DOI： 10.1371/journal.pone.0147570 　

    ISSN： 1932-6203

  46. Establishment of an immortalized cell line derived from the prairie vole via lentivirus-mediated transduction of mutant cyclin-dependent kinase 4, cyclin D, and telomerase reverse transcriptase Peer-reviewed

    Masafumi Katayama, Tohru Kiyono, Kengo Horie, Takashi Hirayama, Takahiro Eitsuka, Kengo Kuroda, Kenichiro Donai, Shizu Hidema, Katsuhiko Nishimori, Tomokazu Fukuda

    EXPERIMENTAL ANIMALS　65　(1)　87-96　2016/01

    DOI： 10.1538/expanim.15-0061 　

    ISSN： 1341-1357

    eISSN： 1881-7122

  47. delta and gamma tocotrienols suppress human hepatocellular carcinoma cell proliferation via regulation of Ras-Raf-MEK-ERK pathway-associated upstream signaling Peer-reviewed

    Gregor Carpentero Burdeos, Junya Ito, Takahiro Eitsuka, Kiyotaka Nakagawa, Fumiko Kimura, Teruo Miyazawa

    FOOD & FUNCTION　7　(10)　4170-4174　2016

    DOI： 10.1039/c6fo00826g 　

    ISSN： 2042-6496

    eISSN： 2042-650X

  48. Metabolism and cytotoxic effects of phosphatidylcholine hydroperoxide in human hepatoma HepG2 cells Peer-reviewed

    Yuuri Suzuki, Kiyotaka Nakagawa, Shunji Kato, Naoto Tatewaki, Shunsuke Mizuochi, Junya Ito, Takahiro Eitsuka, Hiroshi Nishida, Teruo Miyazawa

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　458　(4)　920-927　2015/03

    DOI： 10.1016/j.bbrc.2015.02.063 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  49. Establishment of Cell Lines Derived From the Genus Macaca Through Controlled Expression of Cell Cycle Regulators Peer-reviewed

    Kengo Kuroda, Tohru Kiyono, Takahiro Eitsuka, Hiroshi Isogai, Koichi Takahashi, Kenichiro Donai, Emiko Isogai, Tomokazu Fukuda

    JOURNAL OF CELLULAR BIOCHEMISTRY　116　(2)　205-211　2015/02

    DOI： 10.1002/jcb.24963 　

    ISSN： 0730-2312

    eISSN： 1097-4644

  50. Synergistic inhibition of cancer cell proliferation with a combination of delta-tocotrienol and ferulic acid Peer-reviewed

    Takahiro Eitsuka, Naoto Tatewaki, Hiroshi Nishida, Tadao Kurata, Kiyotaka Nakagawa, Teruo Miyazawa

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　453　(3)　606-611　2014/10

    DOI： 10.1016/j.bbrc.2014.09.126 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  51. Bovine and porcine fibroblasts can be immortalized with intact karyotype by the expression of mutant cyclin dependent kinase 4, cyclin D, and telomerase Peer-reviewed

    Kenichiro Donai, Tohru Kiyono, Takahiro Eitsuka, Yijie Guo, Kengo Kuroda, Hideko Sone, Emiko Isogai, Tomokazu Fukuda

    JOURNAL OF BIOTECHNOLOGY　176　50-57　2014/04

    DOI： 10.1016/j.jbiotec.2014.02.017 　

    ISSN： 0168-1656

    eISSN： 1873-4863

  52. Persimmon leaf flavonols enhanced anti-cancer effec tof heavy ion radiotherapy on murine xenograft tumors. Peer-reviewed

    K. Kawakami, H. Nishida, N. Tatewaki, K. Eguchi-Kasai, K. Anzai, T. Eitsuka, T. Konishi, M. Hirayama

    J Cancer Ther　4　(07)　1150-1157　2013

    Publisher: Scientific Research Publishing, Inc.

    DOI： 10.4236/jct.2013.47133 　

    ISSN： 2151-1934

    eISSN： 2151-1942

  53. Non-thermal DNA damage of cancer cells using near-infrared irradiation Peer-reviewed

    Yohei Tanaka, Naoto Tatewaki, Hiroshi Nishida, Takahiro Eitsuka, Nobuo Ikekawa, Jun Nakayama

    CANCER SCIENCE　103　(8)　1467-1473　2012/08

    DOI： 10.1111/j.1349-7006.2012.02310.x 　

    ISSN： 1347-9032

  54. Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment Peer-reviewed

    Tomokazu Fukuda, Masafumi Katayama, Takayuki Yoshizawa, Takahiro Eitsuka, Hiroaki Mizukami, Kiyotaka Nakagawa, Hisao Ito, Homika Komagata, Sanghoun Song, Sanggun Roh, Yumi Hoshino, Eimei Sato, Hirofumi Hanada, Katsuhiko Nishimori, Teruo Miyazawa, Takafumi Uchida

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　76　(7)　1372-1377　2012/07

    DOI： 10.1271/bbb.120155 　

    ISSN： 0916-8451

    eISSN： 1347-6947

  55. Amadori-glycated phosphatidylethanolamine up-regulates telomerase activity in PANC-1 human pancreatic carcinoma cells Peer-reviewed

    Takahiro Eitsuka, Kiyotaka Nakagawa, Yuichi Ono, Naoto Tatewaki, Hiroshi Nishida, Tadao Kurata, Naoki Shoji, Teruo Miyazawa

    FEBS LETTERS　586　(16)　2542-2547　2012/07

    DOI： 10.1016/j.febslet.2012.06.027 　

    ISSN： 0014-5793

  56. Antiangiogenic and anticancer potential of unsaturated vitamin E (tocotrienol) Invited Peer-reviewed

    Teruo Miyazawa, Akira Shibata, Phumon Sookwong, Yuki Kawakami, Takahlro Eitsuka, Akira Asai, Shinichi Oikawa, Kiyotaka Nakagawa

    JOURNAL OF NUTRITIONAL BIOCHEMISTRY　20　(2)　79-86　2009/02

    DOI： 10.1016/j.jnutbio.2008.09.003 　

    ISSN： 0955-2863

  57. Down-regulation of telomerase activity in DLD-1 human colorectal adenocarcinoma cells by tocotrienol Peer-reviewed

    Takahiro Eitsuka, Kiyotaka Nakagawa, Teruo Miyazawa

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　348　(1)　170-175　2006/09

    DOI： 10.1016/j.bbrc.2006.07.029 　

    ISSN： 0006-291X

  58. Polyunsaturated fatty acids inhibit telomerase activity in DLD-1 human colorectal adenocarcinoma cells: A dual mechanism approach Peer-reviewed

    T Eitsuka, K Nakagawa, T Suzuki, T Miyazawa

    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS　1737　(1)　1-10　2005/10

    DOI： 10.1016/j.bbalip.2005.08.017 　

    ISSN： 1388-1981

  59. Inhibitory effect of conjugated eicosapentaenoic acid on mammalian DNA polymerase and topoisomerase activities and human cancer cell proliferation Peer-reviewed

    Y Yonezawa, T Hada, K Uryu, T Tsuzuki, T Eitsuka, T Miyazawa, C Murakami-Nakai, H Yoshida, Y Mizushina

    BIOCHEMICAL PHARMACOLOGY　70　(3)　453-460　2005/08

    DOI： 10.1016/j.bcp.2005.05.008 　

    ISSN： 0006-2952

  60. Inhibitory effect of conjugated eicosapentaenoic acid on human DNTA topoisomerases I and II Peer-reviewed

    Y Yonezawa, T Tsuzuki, T Eitsuka, T Miyazawa, T Hada, K Uryu, C Murakami-Nakai, H Ikawa, Kuriyama, I, M Takemura, M Oshige, H Yoshida, K Sakaguchi, Y Mizushina

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS　435　(1)　197-206　2005/03

    DOI： 10.1016/j.abb.2004.12.011 　

    ISSN： 0003-9861

  61. Inhibitory action of conjugated C-18-fatty acids on DNA polymerases and DNA topoisomerases Peer-reviewed

    Y Mizushina, T Tsuzuki, T Eitsuka, T Miyazawa, K Kobayashi, H Ikawa, Kuriyama, I, Y Yonezawa, M Takemura, H Yoshida, K Sakaguchi

    LIPIDS　39　(10)　977-983　2004/10

    DOI： 10.1007/s11745-004-1319-y 　

    ISSN： 0024-4201

  62. Telomerase inhibition by sulfoquinovosyldiacylglycerol from edible purple laver (Porphyra yezoensis) Peer-reviewed

    T Eitsuka, K Nakagawa, M Igarashi, T Miyazawa

    CANCER LETTERS　212　(1)　15-20　2004/08

    DOI： 10.1016/j.canlet.2004.03.019 　

    ISSN： 0304-3835

  63. Dual mechanisms for telomerase inhibition in DLD-1 human colorectal adenocarcinoma cells by polyunsaturated fatty acids Peer-reviewed

    T Eitsuka, K Nakagawa, T Miyazawa

    BIOFACTORS　21　(1-4)　19-21　2004

    ISSN： 0951-6433

  64. DNA chip analysis of comprehensive food function: Inhibition of angiogenesis and telomerase activity with unsaturated vitamin E, tocotrienol Peer-reviewed

    K Nakagawa, T Eitsuka, H Inokuchi, T Miyazawa

    BIOFACTORS　21　(1-4)　5-10　2004

    ISSN： 0951-6433

Show all ︎Show first 5

Misc. 67

  1. ニワトリとオオハクチョウ由来線維芽細胞の細胞老化制御の試み

    片山雅史, 清野透, 永塚貴弘, 村山美穂, 大沼学, 福田智一

    日本家禽学会誌　58　2021

    ISSN： 0029-0254

  2. α-トコフェロールとα-トコトリエノールの細胞内取り込み量の違い:培地中のアルブミンによる影響

    中冨毅, 板谷麻由子, 堀越洋輔, 永塚貴弘, 松浦達也, 仲川清隆

    ビタミン　95　(4)　2021

    ISSN： 0006-386X

  3. A study on anticancer effects of tocotrienol and its synergistic action

    94　(12)　2020/12

    ISSN： 0006-386X

  4. Amount of lipid glycation products absorbed into the body of rats

    伊藤隼哉, 梅津直生, 乙木百合香, 加藤俊治, 永塚貴弘, 仲川清隆

    日本メイラード学会学術集会プログラム・抄録集　30th (CD-ROM)　2020

  5. アルツハイマー病モデルマウス(AppNL-G-F mice)における脳内遺伝子発現の加齢による変動

    宮崎苑子, 宮崎苑子, 野間口早紀, 中澤京子, 乙木百合香, 伊藤隼哉, 永塚貴弘, 西道隆臣, 斎藤貴志, 斎藤貴志, 池川繁樹, 仲川清隆, 加藤久典

    栄養学雑誌　78　(5 Supplement)　2020

    ISSN： 0021-5147

  6. 主要リポタンパクに含まれるコレステロールエステルおよびホスファチジルコリンのヒドロペルオキシド異性体解析

    大須賀 佑輔, 加藤 俊治, 伊藤 隼哉, 永塚 貴弘, 仲川 清隆

    脂質生化学研究　61　218-220　2019/06

    Publisher: 日本脂質生化学会

    ISSN： 0285-1520

  7. 研究室紹介 Invited

    伊藤隼哉、永塚貴弘

    オレオサイエンス　19　(6)　264-265　2019/06

  8. LC-MS/MS分析を用いたピロロキノリンキノン存在形態の評価:細胞試験における生理作用発現機構の解明に向けて

    加藤 主税, 伊藤 隼哉, 永塚 貴弘, 仲川 清隆

    ビタミン　93　(4)　213-213　2019/04

    Publisher: (公社)日本ビタミン学会

    ISSN： 0006-386X

  9. ラットにおけるγ-オリザノールの吸収と代謝

    伊藤 隼哉, 國米 匠, 橋本 博之, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本栄養・食糧学会大会講演要旨集　73回　280-280　2019/04

    Publisher: (公社)日本栄養・食糧学会

  10. 腸管吸収過程におけるプラズマローゲンの構造変化

    高橋 巧, 乙木 百合香, 鈴木 拓史, 山下 慎司, 永塚 貴弘, 池田 郁男, 宮澤 陽夫, 仲川 清隆

    日本栄養・食糧学会大会講演要旨集　73回　208-208　2019/04

    Publisher: (公社)日本栄養・食糧学会

  11. サメ肝油中スクアレンの酸化評価とα-トコフェロールによる酸化抑制

    清水 直紀, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 齋藤 哲男, 西田 浩志, 仲川 清隆

    ビタミン　93　(4)　203-203　2019/04

    Publisher: (公社)日本ビタミン学会

    ISSN： 0006-386X

  12. Anti-inflammatory effects of rice bran components Invited

    EITSUKA Takahiro

    Nutrition Reviews　27　(2)　97-106　2019/02

  13. こめ油と含有されるγ-オリザノールの魅力：機能性表示化の動き Invited

    伊藤隼哉、永塚貴弘、仲川清隆

    アグリバイオ　3　865-867　2019

  14. プラズマローゲンの腸管吸収過程における構造変化

    高橋巧, 乙木百合香, 鈴木拓史, 山下慎司, 永塚貴弘, 池田郁男, 宮澤陽夫, 仲川清隆

    日本栄養・食糧学会東北支部大会およびシンポジウム講演要旨集　52nd　25　2018/11/17

  15. Extended life-span of chick fibroblasts by expression of cell cycle regulators

    Masafumi KATAYAMA, Tohru KIYONO, Takahiro EITSUKA, Miho INOUE-MURAYAMA, Manabu ONUMA, Tomokazu FUKUDA

    Program &amp; Abstracts. Annual and International Meeting of the Japanese Association for Animal Cell Technology　31st　137　2018/11

  16. 質量分析を用いたオリーブ油の酸化メカニズム推定

    高橋隼登, 加藤俊治, 加藤俊治, 伊藤隼哉, 境野眞善, 佐野貴士, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本食品科学工学会大会講演集　65th　136　2018/08/22

  17. スクアレンの酸化機構と抗酸化物質によるスクアレン酸化抑制効果の評価

    清水直紀, 伊藤隼哉, 加藤俊治, 加藤俊治, 後藤昌史, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    脂質生化学研究　60　231-233　2018/05

    Publisher: 日本脂質生化学会

    ISSN： 0285-1520

  18. 過酸化リン脂質の立体異性体解析に向けたLC‐IMS‐MS/MS法の開発

    伊藤隼哉, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    脂質生化学研究　60　228-230　2018/05

    Publisher: 日本脂質生化学会

    ISSN： 0285-1520

  19. マウスにおけるこめ油γ‐オリザノールの体内分布:生理作用発現との関係性

    伊藤隼哉, 小林エリ, 加藤俊治, 澤田一恵, 橋本博之, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本栄養・食糧学会大会講演要旨集　72回　200-200　2018/04

    Publisher: (公社)日本栄養・食糧学会

  20. 油脂に含まれるトコフェロール酸化物,とくにトコフェロールヒドロペルオキシドの解析

    丹野玲菜, 加藤俊治, 伊藤隼哉, 小倉由資, 永塚貴弘, 桑原重文, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本栄養・食糧学会大会講演要旨集　72回　296-296　2018/04

    Publisher: (公社)日本栄養・食糧学会

  21. ホスファチジルコリンヒドロペルオキシド(PCOOH)の代謝機構とPCOOHが惹起する細胞死へのビタミンEの影響

    仲川清隆, 勝野真美, 伊藤隼哉, 加藤俊治, 永塚貴弘, 今井浩孝, 宮澤陽夫, 宮澤陽夫

    ビタミン　92　(4)　204-204　2018/04

    Publisher: (公社)日本ビタミン学会

    ISSN： 0006-386X

  22. イオンモビリティーを活用した過酸化リン脂質のLC‐MS/MS立体異性体解析法

    伊藤隼哉, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本農芸化学会大会講演要旨集(Web)　2018　ROMBUNNO.2B06p02 (WEB ONLY)　2018/03/05

    ISSN： 2186-7976

  23. ヒト皮脂中のスクアレン酸化機構の解析および抗酸化物質によるスクアレン酸化抑制効果の評価

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本農芸化学会大会講演要旨集(Web)　2018　ROMBUNNO.2B06p01 (WEB ONLY)　2018/03/05

    ISSN： 2186-7976

  24. ラット胸管リンパカニュレーション試験によるγ‐オリザノールの腸管吸収の評価

    小林エリ, 伊藤隼哉, 澤田一恵, 橋本博之, 加藤俊治, 永塚貴弘, 池田郁男, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本農芸化学会大会講演要旨集(Web)　2018　ROMBUNNO.2B04a12 (WEB ONLY)　2018/03/05

    ISSN： 2186-7976

  25. 6因子発現によるブタiPS細胞のX染色体の活性化と遺伝子発現プロファイリング

    福田 智一, 谷 哲弥, 原口 清輝, 土内 憲一郎, 中嶋 信美, 上西 博英, 永塚 貴弘, 宮川 誠, 宋 相憲, 大沼 学, 星野 由美, 佐藤 英明, 本多 新

    日本畜産学会大会講演要旨集　124回　215-215　2018/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  26. 6因子発現によるブタiPS細胞のX染色体の活性化と遺伝子発現プロファイリング

    福田智一, 谷哲弥, 原口清輝, 土内憲一郎, 中嶋信美, 上西博英, 永塚貴弘, 宮川誠, 宋相憲, 大沼学, 星野由美, 佐藤英明, 本多新

    日本畜産学会大会講演要旨　124回　215-215　2018/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  27. アカウミガメ由来の培養細胞の作製、核型分析、組み換え細胞による細胞増殖の亢進について

    福田智一, 永塚貴弘, 土内憲一郎, 栗田正徳, 斉藤知己, 岡本仁, 木下こずえ, 片山雅史, 日登弘, 内田隆史, 大沼学, 曽根秀子, 村山美穂, 清野透

    第24回日本野生動物医学会大会 2018年8月31日～9月2日 日大阪府立大学 りんくうキャンパス(大阪市）　2018

  28. ホスファチジルコリンヒドロペルオキシド(PCOOH)の代謝機構とPCOOHが惹起する細胞死との関係性

    仲川清隆, 内野正, 伊藤隼哉, 加藤俊治, 永塚貴弘, 今井浩孝, 竹腰進, 宮澤陽夫, 宮澤陽夫

    日本過酸化脂質・抗酸化物質学会年会講演要旨集　26th　9　2018

  29. 第17回日本油化学会オレオサイエンス賞受賞によせて Invited

    永塚 貴弘

    オレオサイエンス　18　402　2018

  30. 粉乳に含まれる脂質糖化産物の分析法の構築と腸管吸収の評価

    伊藤隼哉, 小舘愛, 梅津直生, 乙木百合香, 加藤俊治, 加藤俊治, 池田郁男, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本メイラード学会学術集会プログラム・抄録集　27th　30　2017/11/18

  31. ビタミンE酸化物の検出と抗酸化機序の評価:食用油脂への応用

    丹野玲菜, 加藤俊治, 加藤俊治, 清水直紀, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本食品科学工学会大会講演集　64th　117　2017/08/28

  32. 過酸化リン脂質(PCOOH)が誘導する細胞死メカニズムの検証

    伊藤隼哉, 三上優依, 勝野真美, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    脂質生化学研究　59　37-38　2017/06

    Publisher: 日本脂質生化学会

    ISSN： 0285-1520

  33. 過酸化脂質の異性体解析を通じた皮脂の酸化機構の解明

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    脂質生化学研究　59　253-254　2017/06

    Publisher: 日本脂質生化学会

    ISSN： 0285-1520

  34. リプログラミング6因子の発現によるブタiPS細胞の作製と特徴

    福田智一, 谷哲弥, 原口清輝, 土内憲一郎, 中嶋信美, 上西博英, 永塚貴弘, 宮川誠, 宋相憲, 大沼学, 星野由美, 佐藤英明, 本多新

    日本実験動物学会総会講演要旨集　64th　227　2017/04/28

  35. ビタミンEが過酸化脂質により誘導されるフェロトーシスに与える影響

    伊藤隼哉, 三上優依, 勝野真美, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    ビタミン　91　(4)　265-265　2017/04

    Publisher: (公社)日本ビタミン学会

    ISSN： 0006-386X

  36. ヒト皮脂中のスクアレンヒドロペルオキシド異性体の分析

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本栄養・食糧学会大会講演要旨集　71回　265-265　2017/04

    Publisher: (公社)日本栄養・食糧学会

  37. リノール酸ヒドロペルオキシドとフェロトーシスの関係解明:異性体の違いに着目して

    伊藤隼哉, 三上優依, 勝野真美, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本栄養・食糧学会大会講演要旨集　71回　265-265　2017/04

    Publisher: (公社)日本栄養・食糧学会

  38. リノール酸過酸化物を指標とした食品の酸化機構の評価

    伊藤隼哉, 塩見大樹, 加藤俊治, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本栄養・食糧学会東北支部大会およびシンポジウム講演要旨集　51st　26　2017

  39. アルカリ金属を使用した脂質過酸化物の解析

    加藤俊治, 加藤俊治, 清水直紀, 乙木百合香, 伊藤隼哉, 北谷佳那恵, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 竹腰進, 仲川清隆

    日本過酸化脂質・抗酸化物質学会年会講演要旨集　25th　7　2017

  40. LC‐MS/MSを用いたヒト皮脂中のスクアレン酸化機構の解析

    清水直紀, 伊藤隼哉, 加藤俊治, 加藤俊治, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本過酸化脂質・抗酸化物質学会年会講演要旨集　25th　6　2017

  41. こめ油成分を活用した相乗的ながん抑制効果

    永塚貴弘, 西田浩志, 仲川清隆, 宮澤陽夫

    オレオサイエンス　17　(6)　261-268　2017

    Publisher: 日本油化学会

    ISSN： 1345-8949

  42. 被災地農産物パプリカの機能性の担保に向けて パプリカに含まれるルテオリンの吸収動態の評価

    仲川清隆, 早坂夏実, 永塚貴弘, 庄子真樹, 畑中咲子, 津志田藤二郎, 毛利哲

    農耕と園藝　72　(9)　18-21　2017

    Publisher: 誠文堂新光社

    ISSN： 1345-8833

  43. 脂溶性栄養成分によるがん抑制に関する食品学的研究

    永塚貴弘

    日本栄養・食糧学会誌　70　263-269　2017

    DOI： 10.4327/jsnfs.70.263 　

  44. The 3rd International Conference on Rice Bran Oil 参加報告

    永塚貴弘, 中島成生

    ILSI JAPAN　129　(129)　44-49　2017

    Publisher: 日本国際生命科学協会

    ISSN： 1347-3239

  45. 書評「健康寿命を延ばそう！機能性脂肪酸入門－アルツハイマー病, がん, 糖尿病, 記憶力回復への効果－」

    永塚貴弘, 仲川清隆

    オレオサイエンス　17　340　2017

  46. 学会見聞記 日本農芸化学会 食品（食品機能・栄養）

    永塚貴弘

    バイオサイエンスとインダストリー　75　334-335　2017

  47. 絶滅危惧スイギュウの一種、ローランドアノア由来の無限分裂細胞の作成

    福田 智一, 飯野 佑佳, 永塚 貴弘, 大沼 学, 片山 雅史, 村田 浩一, 村山 美穂, 原 久美子, 清野 透

    東北畜産学会報　66　(2)　22-22　2016/08

    Publisher: 東北畜産学会

    ISSN： 1341-626X

  48. ヒト肝癌細胞HepG2細胞におけるホスファチジルコリンヒドロペルオキシドの代謝と毒性

    鈴木優里, 仲川清隆, 加藤俊治, 舘脇直人, 水落俊介, 伊藤隼哉, 永塚貴弘, 西田浩志, 宮澤陽夫

    ビタミン　90　(5-6)　274-280　2016/06

    Publisher: (公社)日本ビタミン学会

    ISSN： 0006-386X

  49. スクアレンヒドロペルオキシド異性体の高選択的な分析法の構築

    清水直紀, BERSABE Hannah, 伊藤隼哉, 加藤俊治, 十和田諒, 桑原重文, 永塚貴弘, 宮澤陽夫, 宮澤陽夫, 仲川清隆

    日本栄養・食糧学会東北支部大会およびシンポジウム講演要旨集　50th　14　2016

  50. 染色体異常を伴わないローランドアノア線維芽細胞由来の不死化細胞樹立の試み

    飯野佑佳, 清野透, 永塚貴弘, 片山雅史, 土内憲一郎, 黒田健吾, 村田浩一, 大沼学, 福田智一

    日本野生動物医学会大会・講演要旨集　21st　106　2015/07

  51. ヒト肝癌細胞における過酸化リン脂質PCOOHの代謝と作用:PCOOHの作用へのビタミンEの影響

    仲川清隆, 鈴木優里, 加藤俊治, 舘脇直人, 永塚貴弘, 西田浩志, 宮澤陽夫

    ビタミン　89　(4)　216　2015/04/25

    ISSN： 0006-386X

  52. 米粉の先進地・新潟

    永塚貴弘

    JAFS News Letter　21　2015

  53. Squalene Inhibits Inflammation in Tumor Transplanted Mice through the Suppression of PGE(2) Production

    Naoto Tatewaki, Takahiro Eitsuka, Hiroshi Nishida

    FREE RADICAL BIOLOGY AND MEDICINE　76　S133-S133　2014/11

    DOI： 10.1016/j.freeradbiomed.2014.10.216 　

    ISSN： 0891-5849

    eISSN： 1873-4596

  54. 日本農芸化学会大会学会見聞録

    大坪嘉行, 此木敬一, 榎本 賢, 松沢智彦, 永塚貴弘, 都築 毅, 戸部隆太, 渡部 昭, 小酒井 貴晴, 岡澤敦司

    バイオサイエンスとインダストリー　75　(4)　331-340　2014/07/10

    Publisher: バイオサイエンスインダストリー協会

    More details Close

    ISSN 0914-8981

  55. Primary cell culture and immortalized cells from Loggerhead sea turtle, and establishment as the ideal material for the genomic analysis with next generation sequencing.

    Fukuda T, Donai K, Eitsuka T, Kurita M, Saito T, Okamoto H, Kinoshita K, Katayama M, Soichi M, Uchida T, Onuma M, Sone H, Inoue-Murayama M, Kiyono T

    Unraveling Biodiversity from DNA: -From the Management of Databases to the Use of Next Generation Sequencers- 2014年9月19日、つくば　2014

  56. Squalene inhibits DNA damage response through the induction of Wip1 phosphatase in A549 adenocarcinoma cells

    N. Tatewaki, T. Eitsuka, T. Konishi, H. Nishida

    MOLECULAR BIOLOGY OF THE CELL　24　2013

    ISSN： 1059-1524

    eISSN： 1939-4586

  57. Strawberry polyphenols attenuate the production of inflammatory mediators in U937 cells

    M. Saito, M. Nishida, M. Kokubo, T. Eitsuka, H. Nishida

    MOLECULAR BIOLOGY OF THE CELL　24　2013

    ISSN： 1059-1524

    eISSN： 1939-4586

  58. Amadori-glycated phosphatidylethanolamineincreases cellular telomerase activity: A link between diabetes and cancer

    T. Eitsuka, K. Nakagawa, H. Nishida

    IMARS Highlights　8　24-25　2013

  59. 他学会だよりThe 6th International Niigata Symposium on Diet and Health に参加して

    永塚貴弘

    JMARS News Letter　3-4　2013

  60. 国際交流助成報告 Diet and Optimum Health

    永塚貴弘

    日本栄養・食糧学会誌　66　(6)　327　2013

  61. Antiangiogenic effects of tocotrienol

    Takahiro Eitsuka, Kiyotaka Nakagawa, Teruo Miyazawa

    Tocotrienols: Vitamin E beyond Tocopherols, Second Edition　79-88　2012/01/01

    DOI： 10.1201/b12502 　

  62. 最近の文献紹介

    西田浩志, 永塚貴弘

    JMARS News Letter　5-6　2011

  63. 米ぬかトコトリエノールと他成分による相乗的生理作用向上の機構解明

    仲川清隆, 永塚貴弘, 川上祐生

    飯島記念食品科学振興財団年報　2008　2010

    ISSN： 1346-0625

  64. 米ぬかトコトリエノールと他成分による相乗的生理作用向上の機構解明—Study on synergistic effect of tocotrienol and other constituents in rice bran

    仲川 清隆, 永塚 貴弘, 川上 祐生

    年報 / 飯島記念食品科学振興財団 [編] = Annual report / The Iijima Memorial Foundation for the Promotion of Food Science and Technology　2008年度　248-252　2008

    Publisher: 市川 : 飯島記念食品科学振興財団

    ISSN： 1346-0625

  65. Study on synergistic effect of tocotrienol and other constituents in rice bran

    2008　248-252　2008

    Publisher: 飯島記念食品科学振興財団

    ISSN： 0913-2341

  66. Telomerase inhibition

    T. Eitsuka, K. Nakagawa, T. Miyazawa

    Tocotrienols: Vitamin E Beyond Tocopherols　209-218　2008

  67. Polyunsaturated fatty acids inhibit telomerase activity in DLD-1 human colorectal adenocarcinoma cells: a dual mechanism approach. International-journal

    Takahiro Eitsuka, Kiyotaka Nakagawa, Toshihide Suzuki, Teruo Miyazawa

    Biochimica et biophysica acta　1737　(1)　1-10　2005/10/15

    ISSN： 0006-3002

    More details Close

    As high telomerase activity is detected in most cancer cells, telomerase represents a promising cancer therapeutic target. We investigated the inhibitory effect of various fatty acids on telomerase, with particular emphasis on those with antitumor properties, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). To evaluate the direct effect of fatty acids on telomerase, cell lysates of DLD-1 human colorectal adenocarcinoma cells were mixed with sample fatty acids, and the telomerase activity was determined. Saturated fatty acids and trans-fatty acids showed very weak or no inhibition of telomerase. In contrast, cis-unsaturated fatty acids significantly inhibited the enzyme, and the inhibitory potency was elevated with an increase in the number of double bonds. Accordingly, polyunsaturated fatty acids (PUFAs), like EPA and DHA, appeared to be powerful telomerase inhibitors. To assess the transcriptional effect, DLD-1 cells were cultured in the presence of sample fatty acids, and telomerase activity and gene expression were subsequently evaluated. Culturing DLD-1 cells with either EPA or DHA resulted in a remarkable decrease in telomerase activity. EPA and DHA inhibited telomerase by down-regulating human telomerase reverse transcriptase (hTERT) and c-myc expression via protein kinase C inhibition. These results indicate that PUFAs can directly inhibit the enzymatic activity of telomerase as well as modulate the telomerase at the transcriptional level.

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Books and Other Publications 8

  1. 脂質・脂肪酸関連物質の使いこなし方 : 素材開発・機能創生・応用技術

    永塚貴弘

    2020/09

    ISBN: 9784924728868

  2. 食品の機能化学

    永塚貴弘

    2020/06

  3. 食品機能性成分の吸収・代謝・作用機序

    永塚 貴弘

    シーエムシー出版　2018/04

  4. 食品機能性成分の吸収・代謝機構

    永塚貴弘

    シーエムシー出版　2013

  5. 機能性食品の作用と安全性百科

    永塚貴弘, 仲川清隆

    丸善出版　2012

  6. 新訂 食品の機能化学

    永塚貴弘

    アイ・ケイ コーポレーション　2010

  7. 新訂 食品の機能化学

    永塚貴弘

    アイ・ケイ コーポレーション　2010

  8. 新訂 食品の機能化学

    永塚貴弘

    アイ・ケイ コーポレーション　2010

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Presentations 83

  1. トコフェロールとトコトリエノールのアルブミン複合体の細胞内取り込み量の違いのメカニズム

    中冨毅, 堀越洋輔, 髙橋麻由子, 永塚貴弘, 松浦達也, 仲川清隆

    お米の未来を考えるシンポジウム ～これまでのお米、これからのお米。～

  2. ヒツジ筋肉由来線維芽細胞の無限分裂および生物学的特徴の解明

    福田 智一, 菊地 乃愛, 松坂 ひまり, 白 蘭蘭, 佐野 宏明, 永塚 貴弘, 仲川 清隆, 菅野 江里子, 尾﨑 拓, 冨田 浩史, 清野 透

    日本畜産学会大131回大会　2023/09/20

  3. Lipid oxidation stress: Botanical anthioxidant strategies for health

    Kiyotaka Nakagawa, Jina Hong, Arun Rajgopal, Hirono Totsuka, Junya Ito, Shunji Kato, Takahiro Eitsuka

    14th Asian Congress of Nutrition

  4. トコフェロールとトコトリエノールの細胞内取り込み量の違い：アルブミンとの結合を介したメカニズムの解析

    中冨毅, 堀越洋輔, 髙橋麻由子, 永塚貴弘, 松浦達也, 仲川清隆

    第65回日本脂質生化学会

  5. 絶滅危惧種アマミノクロウサギ由来の無限分裂齊藤および体細胞クローンの作製

    福田智一, 折本愛, 片山雅史, 谷哲弥, 伊藤圭子, 永塚貴弘, 仲川清隆, 村山美穂, 大沼学, 清野透

    日本畜産学会第128回大会　2021/03/28

  6. ニワトリとオオハクチョウ由来線維芽細胞の細胞老化制御の試み

    片山雅史, 清野透, 永塚貴弘, 村山美穂, 大沼学, 福田智一

    日本家禽学会 2021 年度春季大会　2021/03/27

  7. 1-Deoxynojirimycin from Bacillus amyloliquefaciens AS385 ameliorates high-fat diet-induced metabolic disorders in C57BL/6J mice

    Isabella Supardi Parida, 高須蒼生, 伊藤隼哉, 池田亮一, 山岸賢治, 木村俊之, 永塚貴弘, 仲川清隆

    日本栄養・食糧学会 東北支部第53回支部大会およびシンポジウム　2020/10/31

  8. トコトリエノールのがん抑制作用とその高機能化に関する研究 Invited

    永塚貴弘

    日本ビタミン学会第72回大会　2020/06/19

  9. プラズマローゲンの細胞内動態と神経保護作用の評価

    熊谷桂, 乙木百合香, 伊藤隼哉, 仲川清隆, 永塚貴弘

    第74回日本栄養・食糧学会大会　2020/05/16

  10. スクアレン酸化物の合成・構造解析とヒト表皮角化細胞HaCaTに与える影響の評価

    Khalifa Saoussane, 加藤俊治, 清水直紀, 伊藤隼哉, 永塚貴弘, 仲川清隆

    第74回日本栄養・食糧学会大会　2020/05/16

  11. 脂質メイラード産物の体内吸収の評価: LC-MS/MS を用いた解析を通して

    伊藤隼哉, 梅津直生, 乙木百合香, 加藤俊治, 永塚貴弘, 仲川清隆

    第74回日本栄養・食糧学会大会　2020/05/16

  12. 細胞周期制御因子を用いたニワトリ線維芽細胞の細胞寿命制御

    片山 雅史, 清野透, 永塚貴弘, 村山美穂, 大沼学, 福田智一

    日本家禽学会 2020 年度春季大会　2020/03/28

  13. Squalene oxidation products and their effect on the human keratinocyte cell line HaCaT

    Saoussane Khalifa, Naoki Shimizu, Shunji Kato, Junya Ito, Takahiro Eitsuka, Kiyotaka Nakagawa

    日本農芸化学会2020年度大会　2020/03/27

  14. Squalene oxidation and its effect on human keratinocyte cell line HaCaT

    Saoussane Khalifa, Naoki Shimizu, Junya Ito, Takahiro Eitsuka, Kiyotaka Nakagawa

    The 7th International Conference on Food Factors (ICoFF 2019)　2019/12/03

  15. Screening of compounds that inhibit the interaction between curcumin and bovine serum albumin to improve the physiological activity of curcumin

    Mayuko Itaya, Taiki Miyazawa, Takahiro Eitsuka, Atsushi Imaizumi, Kiyotaka Nakagawa

    The 7th International Conference on Food Factors (ICoFF 2019)　2019/12/03

  16. プラズマローゲンの細胞内動態と神経保護作用

    熊谷桂, 乙木百合香, 伊藤隼哉, 仲川清隆, 永塚貴弘

    日本栄養・食糧学会東北支部 (第53回大会)　2019/11/17

  17. ヒト皮脂中のスクアレンの酸化機構解析

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚貴弘, 仲川清隆

    日本農芸化学会東北支部第154大会　2019/11/09

  18. LC-MS/MSを用いた脂質糖化産物の腸管吸収の評価

    梅津直生, 伊藤隼哉, 加藤俊治, 永塚貴弘, 仲川清隆

    第29回日本メイラード学会年会　2019/10/27

  19. リノール酸由来の6種のヒドロペルオキシド異性体とグリシンのメイラード反応

    仲川清隆, 伊藤隼哉, 加藤俊治, 永塚貴弘

    第29回日本メイラード学会年会　2019/10/27

  20. カチオン-π電子相互作用に基づく脂質ヒドロペルオキシドの異性体解析

    加藤俊治, 乙木百合香, 伊藤隼哉, 永塚貴弘, 仲川清隆

    日本油化学会第58回年会　2019/09/25

  21. Physiological Effects and Organ Distribution of 1-Deoxynojirimycin From Bacillus amyloliquefaciens AS385 in C57Bl/6J Mice

    Isabella Supardi Parida, Soo Takasu, Junya Ito, Ryoichi Ikeda, Kenji Yamagishi, Toshiyuki Kimura, Teruo Miyazawa, Takahiro Eitsuka, Kiyotaka Nakagawa

    Asian Congress of Nutrition 2019　2019/08/05

  22. Evaluation of γ-Oryzanol Accumulation and Lipid Metabolism of Mouse Following A Long-Term Administration of γ-Oryzanol

    Asian Congress of Nutrition 2019　2019/08/05

  23. 主要リポタンパクに含まれるコレステロールエステルおよびホスファチジルコリンのヒドロペルオキシド異性体解析

    大須賀佑輔, 加藤俊治, 伊藤隼哉, 永塚貴弘, 仲川清隆

    第61回 日本脂質生化学会　2019/07/05

  24. サメ肝油中スクアレンの酸化評価とα-トコフェロールによる酸化抑制

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚貴弘, 齋藤哲男, 西田浩志, 仲川清隆

    日本ビタミン学会第71回大会　2019/06/08

  25. LC-MS/MS分析を用いたピロロキノリンキノン存在形態の評価：細胞試験における生理作用発現機構の解明に向けて

    加藤主税, 伊藤隼哉, 永塚貴弘, 仲川清隆

    日本ビタミン学会第71回大会　2019/06/08

  26. ラットにおける γ-オリザノールの吸収と代謝

    伊藤隼哉, 國米匠, 橋本博之, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第73回日本栄養・食糧学会大会　2019/05/18

  27. クルクミノイドと血清アルブミンの結合を競合的に阻害できる成分の探索

    板谷麻由子, 宮澤大樹, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第73回日本栄養・食糧学会大会　2019/05/18

  28. 腸管吸収過程におけるプラズマローゲンの構造変化

    高橋巧, 乙木百合香, 鈴木拓史, 山下慎司, 永塚貴弘, 池田郁男, 宮澤陽夫, 仲川清隆

    第73回日本栄養・食糧学会大会　2019/05/18

  29. ピロロキノリンキノン（PQQ）のLC-MS/MS分析：牛乳およびヒト母乳の測定

    加藤主税, 河合笑子, 小林俊二郎, 伊藤隼哉, 永塚貴弘, 仲川清隆

    日本農芸化学会2019年度大会　2019/03/24

  30. LC-MS/MSを用いたオリーブ油の酸化メカニズム推定

    高橋隼登, 加藤俊治, 伊藤隼哉, 境野眞喜, 佐野貴士, 永塚貴弘, 宮澤陽夫, 仲川清隆

    日本農芸化学会2019年度大会　2019/03/24

  31. 質量分析を用いた油脂初期酸化の新規評価法

    加藤俊治, 伊藤隼哉, 境野眞善, 佐野貴士, 永塚貴弘, 宮澤陽夫, 仲川清隆

    日本農芸化学会2019年度大会　2019/03/24

  32. γ-オリザノールの吸収代謝：生理作用発現との関係解明に向けて

    國米匠, 小林エリ, 伊藤隼哉, 橋本博之, 永塚貴弘, 宮澤陽夫, 仲川清隆

    平成30年度第3回油化学セミナー　2019/01/25

  33. 製造や保存、調理におけるオリーブ油の酸化：酸化抑制へのビタミンEの寄与

    高橋隼登, 加藤俊治, 伊藤隼哉, 境野眞善, 佐野貴士, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第30回ビタミンＥ研究会　2019/01/12

  34. ロールドオーツ由来の未利用副産物における脂質やビタミンE等の機能性成分

    樊欣熠, 清水直紀, 高橋巧, 糸山隆一, 石原克之, 伊藤隼哉, 永塚貴弘, 仲川清隆

    第30回ビタミンＥ研究会　2019/01/12

  35. 油脂中のトコフェロール酸化物、とくにヒドロペルオキシド型の解析

    丹野玲菜, 加藤俊治, 伊藤隼哉, 小倉由資, 永塚貴弘, 桑原重文, 宮澤陽夫, 仲川清隆

    第30回ビタミンＥ研究会　2019/01/12

  36. α-トコフェロールによるスクアレンの酸化抑制と抑制機構の評価

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚隆弘, 宮澤陽夫, 仲川清隆

    第30回ビタミンＥ研究会　2019/01/12

  37. Physiological Effects and Organ Distribution of Microbial 1-Deoxynojirimycin in C57BL/6J Mice International-presentation

    Isabella Supardi Parida, Soo Takasu, Junya Ito, Ryoichi Ikeda, Kenji Yamagishi, Toshiyuki Kimura, Teruo Miyazawa, Takahiro Eitsuka, Kiyotaka Nakagawa

    Asia-Pacific Nutrigenomics and Nutrigenetics Organization (APNNO) 2018　2018/12/02

  38. Squalene Oxidation Mechanisms in Skin Surface Lipids and Its Prevention by Antioxidants International-presentation

    Naoki Shimizu, Junya Ito, Shunji Kato, Tomomi Ayasu, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    Asia-Pacific Nutrigenomics and Nutrigenetics Organization (APNNO) 2018　2018/12/02

  39. Determination of Triacylglycerol Oxidation Mechanisms Using Liquid Chromatography-tandem Mass Spectrometry International-presentation

    Shunji Kato, Naoki Shimizu, Yurika Otoki, Junya Ito, Masayoshi Sakaino, Takashi Sano, Takahiro Eitsuka, Susumu Takekoshi, Teruo Miyazawa, Kiyotaka Nakagawa

    The 3rd International Symposium on Rice Science in Global Health　2018/11/29

  40. Evaluation and prevention of Squalene Oxidation International-presentation

    Naoki Shimizu, Junya Ito, Shunji Kato, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    The 3rd International Symposium on Rice Science in Global Health　2018/11/29

  41. プラズマローゲンの腸管吸収過程における構造変化

    髙橋 巧, 乙木 百合香, 鈴木 拓史, 山下 慎司, 永塚 貴弘, 池田 郁男, 宮澤 陽夫, 仲川 清隆

    日本栄養・食糧学会北海道支部東北支部合同支部大会（第52回日本栄養・食糧学会東北支部大会）　2018/11/17

  42. LC-MS/MSを用いたγ-オリザノールの経口摂取時における血中フェルラ酸および代謝物の解析

    國米 匠, 伊藤 隼哉, 橋本 博之, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    平成30年度日本食品科学工学会 東北支部大会および市民フォーラム　2018/10/13

  43. クルクミノイドの細胞内取り込み機構の解明とその制御に向けて

    板谷 麻由子, 宮澤 大樹, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本農芸化学会東北・北海道合同支部大会（東北支部第153回大会）　2018/09/22

  44. スクアレン（SQ）の酸化機構の解明と抗酸化物質によるSQ酸化の抑制

    清水 直紀, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本農芸化学会東北・北海道合同支部大会（東北支部第153回大会）　2018/09/22

  45. イオンモビリティーMS（IMMS）による過酸化脂質の立体異性体分離

    伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本農芸化学会東北・北海道合同支部大会（東北支部第153回大会）　2018/09/22

  46. こめ油成分による相乗的な癌細胞増殖阻害

    永塚 貴弘, 仲川 清隆, 宮澤 陽夫

    日本油化学会第57回年会　2018/09/04

  47. ラットへの γ- オリザノールの経口投与と血中フェルラ酸および代謝物の分析

    國米 匠, 伊藤 隼哉, 橋本 博之, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本油化学会第57回年会　2018/09/04

  48. 質量分析を用いたオリーブ油の酸化メカニズム推定

    高橋 隼登, 加藤 俊治, 伊藤 隼哉, 境野 眞善, 佐野 貴士, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本食品科学工学会第65回大会　2018/08/22

  49. 米に特有と考えられていた γ-オリザノール分子種を大麦から検出

    伊藤 隼哉, Fan Xinyi, 橋本 博之, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本食品科学工学会第65回大会　2018/08/22

  50. ロールドオーツ由来の未利用副産物に含まれる機能性成分の解析

    Fan Xinyi, 清水 直紀, 高橋 巧, 糸山 隆一, 石原 克之, 伊藤 隼哉, 永塚 貴弘, 仲川 清隆

    日本食品科学工学会第65回大会　2018/08/22

  51. ホスファチジルコリンヒドロペルオキシド（PCOOH）の代謝機構とPCOOHが惹起する細胞死との関係性

    仲川 清隆, 内野 正, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 今井 浩孝, 竹腰 進, 宮澤 陽夫

    日本過酸化脂質・抗酸化物質学会第26回年会　2018/08/19

  52. ホスファチジルコリンヒドロペルオキシド（PCOOH）の代謝機構とPCOOHが惹起する細胞死へのビタミンEの影響

    仲川 清隆, 勝野 真美, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 今井 浩孝, 宮澤 陽夫

    日本ビタミン学会第70回大会　2018/06/22

  53. クルクミノイドの細胞内取り込み機構の解明に向けて：細胞外物質の影響

    板谷 麻由子, 宮澤 大樹, Jean-Marc Zingg, 永塚 貴弘, Mohsen Meydani, Angelo Azzi, 宮澤 陽夫, 仲川 清隆

    日本ビタミン学会第70回大会　2018/06/22

  54. スクアレンの酸化機構と抗酸化物質によるスクアレン酸化抑制効果の評価

    清水 直紀, 伊藤 隼哉, 加藤 俊治, 後藤 昌史, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    第60回 日本脂質生化学会　2018/05/31

  55. 過酸化リン脂質の立体異性体解析に向けた LC-IMS-MS/MS 法の開発

    伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    第60回 日本脂質生化学会　2018/05/31

  56. 油脂に含まれるトコフェロール酸化物、とくにトコフェロールヒドロペルオキシドの解析

    丹野 玲菜, 加藤 俊治, 伊藤 隼哉, 小倉 由資, 永塚 貴弘, 桑原 重文, 宮澤 陽夫, 仲川 清隆

    第72回日本栄養・食糧学会大会　2018/05/11

  57. マウスにおけるこめ油γ-オリザノールの体内分布：生理作用発現との関係性

    伊藤 隼哉, 小林 エリ, 加藤 俊治, 澤田 一恵, 橋本 博之, 永塚 貴弘, 宮澤 陽夫, 仲川 清

    第72回日本栄養・食糧学会大会　2018/05/11

  58. イオンモビリティーを活用した過酸化リン脂質のLC-MS/MS立体異性体解析法

    伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本農芸化学会2018年度（平成30年度）大会　2018/03/15

  59. ヒト皮脂中のスクアレン酸化機構の解析および抗酸化物質によるスクアレン酸化抑制効果の評価

    清水 直紀, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆

    日本農芸化学会2018年度（平成30年度）大会　2018/03/15

  60. ラット胸管リンパカニュレーション試験によるγ-オリザノールの腸管吸収の評価

    小林 エリ, 伊藤 隼哉, 澤田 一恵, 橋本 博之, 加藤 俊治, 永塚 貴弘, 池田 郁男, 宮澤 陽夫, 仲川 清隆

    日本農芸化学会2018年度（平成30年度）大会　2018/03/15

  61. トコトリエノールとフェルラ酸による相乗的な癌抑制作用

    永塚貴弘, 仲川清隆, 宮澤陽夫

    第29回ビタミンE研究会　2018/01/27

  62. トコフェロール酸化物の検出と抗酸化機序の評価：食用油脂への応用

    丹野玲菜, 加藤俊治, 清水直紀, 伊藤隼哉, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第29回ビタミンE研究会　2018/01/27

  63. 粉乳に含まれる脂質糖化産物の分析法の構築と腸管吸収の評価

    伊藤隼哉, 小舘愛, 梅津直生, 乙木百合香, 加藤俊治, 池田郁男, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第27回日本メイラード学会年会　2017/11/18

  64. 脂溶性栄養成分による癌抑制に関する食品学的研究

    第51回日本栄養・食糧学会東北支部大会　2017/10/14

  65. リノール酸過酸化物を指標とした食品の酸化機構の評価

    伊藤隼哉, 塩見大樹, 加藤俊治, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第51回日本栄養・食糧学会東北支部大会　2017/10/14

  66. Determination of triacylglycerol oxidation mechanisms using liquid chromatography-mass spectrometrytandem International-presentation

    Shunji Kato, Naoki Shimizu, Yasuhiko Hanzawa, Yurika Otoki, Junya Ito, Fumiko Kimura, Susumu Takekoshi, Masayoshi Sakaino, Takashi Sano, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    The Asian Conference on Oleo Science 2017 (ACOS2017)　2017/09/11

  67. ビタミンE酸化物の検出と抗酸化機序の評価：食用油脂への応用

    丹野玲菜, 加藤俊治, 清水直紀, 永塚貴弘, 宮澤陽夫, 仲川清隆

    日本食品科学工学会第64回大会　2017/08/28

  68. クルクミノイドの細胞内吸収の違い：生理作用発現機構の解明に向けて

    板谷麻由子, 宮澤大樹, Jean-Marc Zingg, 永塚貴弘, Mohsen Meydani, Angelo Azzi, 宮澤 陽夫, 仲川清隆

    日本食品科学工学会第64回大会　2017/08/28

  69. Suppression of angiogenesis and telomerase by tocotrienol International-presentation

    4th International Conference on Rice Bran Oil (ICRBO 2017)　2017/08/24

  70. LC-MS/MSを用いたヒト皮脂中のスクアレン酸化機構の解析

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚貴弘, 宮澤陽夫, 仲川清隆

    日本過酸化脂質・抗酸化物質学会第25回年会　2017/08/19

  71. アルカリ金属を使用した脂質過酸化物の解析

    加藤俊治, 清水直紀, 乙木百合香, 伊藤隼哉, 北谷佳那恵, 永塚貴弘, 宮澤陽夫, 竹越進, 仲川清隆

    日本過酸化脂質・抗酸化物質学会第25回年会　2017/08/19

  72. ホスファチジルコリンヒドロペルオキシドの細胞毒性に及ぼす抗酸化物質の影響

    内野正, 仲川清隆, 勝野真美, 伊藤隼哉, 永塚貴弘, 宮澤陽夫, 秋山卓美, 五十嵐良明

    日本過酸化脂質・抗酸化物質学会第25回年会　2017/08/19

  73. HPLC-MS and HPLC-UV/V is analysis of lutein in mugwort paste International-presentation

    Marina Komuro, Naoki Shimizu, Ryo Onuma, Yurika Otoki, Junya Ito, Shunji Kato, Ohki Higuchi, K Sudo, S Suzuki, Teruo Miyazawa, Takahiro Eitsuka, Kiyotaka Nakagawa

    The Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  74. Detection of oxidation products of vitamin E and evaluation of anti-oxidation mechanisms: Application to edible oils International-presentation

    Rena Tanno, Shunji Kato, Naoki Shimizu, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    The Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  75. Investigation of the mechanisms of differential cellular uptake of curcuminoids International-presentation

    Mayuko Itaya, Taiki Miyazawa, Jean Marc Zingg, Takahiro Eitsuka, Mohsen Meydani, Angelo Azzi, Teruo Miyazawa, Kiyotaka Nakagawa

    The Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  76. LC-MS/MS analysis of squalene hydroperoxide isomers in human skin surface lipids International-presentation

    Naoki Shimizu, Junya Ito, Shunji Kato, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa

    The Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  77. 過酸化脂質の異性体解析を通じた皮脂の酸化機構の解明

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第59回日本脂質生化学会　2017/06/15

  78. 過酸化リン脂質（PCOOH）が誘導する細胞死メカニズムの検証

    伊藤隼哉, 三上優依, 勝野真美, 加藤俊治, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第59回日本脂質生化学会　2017/06/15

  79. ビタミン E が過酸化脂質により誘導されるフェロトーシスに与える影響

    伊藤隼哉, 三上優依, 勝野真美, 加藤俊治, 永塚貴弘, 宮澤陽夫, 仲川清隆

    日本ビタミン学会第69回大会　2017/06/09

  80. 脂溶性栄養成分による癌抑制に関する食品学的研究

    第71回日本栄養・食糧学会大会　2017/05/19

  81. ヒト皮脂中のスクアレンヒドロペルオキシド異性体の分析

    清水直紀, 伊藤隼哉, 加藤俊治, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第71回日本栄養・食糧学会大会　2017/05/19

  82. リノール酸ヒドロペルオキシドとフェロトーシスの関係解明：異性体の違いに着目して

    伊藤隼哉, 三上優依, 勝野真美, 加藤俊治, 永塚貴弘, 宮澤陽夫, 仲川清隆

    第71回日本栄養・食糧学会大会　2017/05/19

  83. 米ぬか成分を活用した相乗的な癌細胞増殖阻害

    永塚貴弘, 舘脇直人, 西田浩志, 仲川清隆, 宮澤陽夫

    日本農芸化学会2017年度（平成29年度）大会　2017/03/17

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Research Projects 14

  1. 食品成分の新規機能性評価とその分子機構解明 Competitive

    2017/04 - Present

  2. Peroxide-omicsの体系化で脂質酸化の全貌の先(食品の未来品質・ヒトの健康社会)へ迫る

    仲川 清隆, 永塚 貴弘, 伊藤 隼哉, 加藤 俊治, 乙木 百合香

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2025/04/01 - 2028/03/31

  3. Induction of cellular senescence by lipid hydroperoxides and its regulation by food components

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2023/04/01 - 2026/03/31

  4. 脂質酸化全貌情報の精緻化から創造する食品の未来品質・ヒトの健康社会

    仲川 清隆, 永塚 貴弘, 加藤 俊治, 伊藤 隼哉, 乙木 百合香

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

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    食品劣化や疾病に繋がる脂質酸化のメカニズムの全貌解明は、世界の過酸化脂質研究者の究極目標である。我々は独自のLC-MS/MS法を駆使し、ラジカル酸化や酵素酸化、一重項酸素酸化、それぞれから特徴的に生成する脂質ヒドロペルオキシドの構造に厳密に応じて、さらに多種多様で膨大な酸化二次生成物が生じることを確信し、この情報の精緻化で脂質酸化経路の全貌を捉えられると着想した。そして、マップを活かし特定の経路を制御し、食品の未来品質・ヒトの健康社会に繋げることを発想して、これを具現化する本研究を構想した。これらの課題を達成し、脂質酸化の徹底管理による食の安全性担保とフードロス削減、酸化物の構造制御によるヒトを魅了する味・香りのプロデュース、そして疾病の新規診断・治療技術の開発やその予防に向けた新機能性食品の創成へ結びつける。こうした基盤的研究を推進し、本研究領域に関する未来社会（プレシンギュラリティ時代）へ立ち向かえる基盤価値を創造する。 上記の課題達成のため、本研究では4つの課題（課題A, 課題B-1, 課題B-2, 課題C）を設定し、本年度は課題A、課題B-1に加え、課題B-2に取り組んだ。また、課題Cについて前年度の結果をベースにさらに研究を進めた。

  5. 脂質酸化全貌情報の精緻化から創造する食品の未来品質・ヒトの健康社会

    仲川 清隆, 永塚 貴弘, 加藤 俊治, 伊藤 隼哉, 乙木 百合香

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  6. Effect of lipid hydroperoxide on cancer progression and its mechanism of action

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2020/04/01 - 2023/03/31

  7. Elucidation of the lipid oxidation mechanisms and their optimal control leading to a healthy- and food-based society of the future through the analysis of lipid hydroperoxides isomers

    Nakagawa Kiyotaka

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    Lipid molecules in vivo are oxidized by several mechanisms (e.g., radical oxidation, enzymatic oxidation, and singlet oxygen oxidation), and the formation of lipid hydroperoxides leads to disturbances in biological functions. Oxidative degradation of oils/fats in foods is deeply related to food quality. Therefore, it is vital to determine oxidative mechanisms in vivo and food products. We have attempted to identify the lipid oxidation mechanisms by analyzing lipid hydroperoxides at the isomeric level using LC-MS/MS. In this study, we tried to develop analytical methods for lipid hydroperoxides and applied them to a wide range of biological and food samples. In addition, we have strived to elucidate the degradation mechanisms of these hydroperoxides.

  8. Challenges into the definite control of human odor by regulating the oxidation of a newly conceptualized terpenoid "squalene"

    Nakagawa Kiyotaka

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2019/06/28 - 2021/03/31

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    To prove that human odor is derived from the oxidation of squalene, we conducted (1) evaluation of the oxidation of squalene and fatty acids in human sebum, (2) analysis of odor components derived from squalene oxidation products, and (3) screening of antioxidants effective towards the regulation of human odor via controlling squalene oxidation. From (1), we were able to identify that squalene oxidation is a potential cause of human odor as in the known case of fatty acids. In (2), we analyzed the chemical structures of odor components derived from the oxidation of squalene, and partially estimated their generation pathways. In (3), we identified antioxidants that are effective towards preventing squalene oxidation, which potentially may be effective towards controlling human odor.

  9. Synergistic anti-cancer effects of rice bran components and their mechanisms of action

    Eitsuka Takahiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

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    Rice bran consists of many functional compounds, including phytosterols, vitamin E (tocopherol and tocotrienol), ferulic acid, and gamma-oryzanol. We clarified the synergistic anti-cancer action (cell growth suppression and telomerase inhibition) of tocotrienol and ferulic acid. This synergistic effect is attributed to an increase in the cellular concentration of tocotrienol through inhibition of its metabolism by ferulic acid. Ferulic acid analogs containing a phenylpropanoid backbone can also synergize with tocotrienol. Our findings suggest the application of rice bran components to cancer prevention and therapy.

  10. Elucidation of the mechanism for lipid hydroperoxide generation, its involvement in diseases, and its suppression by dietary components

    Kiyotaka Nakagawa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    We have developed analytical methods of measuring lipid hydroperoxides in vivo and elucidated the link between lipid hydroperoxides and several diseases. In this study, sensitive and selective methods were established for detection of individual molecular species of fatty acid hydroperoxides and stereoisomer of phospholipid hydroperoxides. Using these methods, we clarified the mechanism for lipid oxidation induced by high-fat diet in animal study, and also revealed that ferroptosis, a newly discovered type of programmed cell death, is involved in deterioration of cell function by lipid hydroperoxides and the cell death is attenuated by α-tocopherol treatment.

  11. Elucidation of new physiological functions such as anti-obesity and anti-inflammation of food Maillard reaction products

    Miyazawa Teruo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2013/04/01 - 2018/03/31

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    In this study, the in vivo physiological effects (i.e. alleviation of hypertension and oxidative stress) of soy sauce, a Japanese food containing food Maillard reaction products, was examined in an animal study. Additionally, the absorption mechanism of food Maillard reaction products were evaluated, since understanding such mechanisms are important to identify how such physiological functions are expressed in vivo. Using Amadori-PE as a marker of food Maillard reaction products, it was revealed that Amadori-PE surely move into the body via intestinal absorption. Moreover, as a new perspective, we developed a novel liposome that utilizes food Maillard reaction products as a base material for nanoparticles (liposomes); such liposomes demonstrated high stability and excellent uptake ability into cells.

  12. Up-regulation of cellular telomerase activity by Amadori-glycated phosphatidylethanolamine: a link between diabetes and cancer

    Eitsuka Takahiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Nigata University of Phermacy and Applied Life Sciences

    2013/04/01 - 2016/03/31

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    Numerous epidemiologic studies suggest that type 2 diabetes significantly increases cancer risk. Amadori-glycated phosphatidylethanolamine (Amadori-PE) plays a key role in the development of diabetic complications. We hypothesized that Amadori-PE may participate not only in the pathogenesis of diabetic complications but also in tumor progression. In the present study, this hypothesis was investigated in cell-culture study, with particular emphasis on the effect of Amadori-PE on telomerase activity which contributes to the infinite replicative potential of cancer cells. Amadori-PE activated MAPK pathway by inducing oxidative stress, leading to up-regulation of c-Myc and hTERT expressions, thereby increasing cellular telomerase activity. Telomerase activity was decreased by a combination treatment of Amadori-PE and alpha-tocopherol, an antioxidant vitamin. These results provide experimental evidence for a novel role of lipid glycation in linking diabetes and cancer.

  13. Telomerase inhibition by carotenoid and its synergistic effect of other constituents in food stuffs.

    EITSUKA Takahiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Nigata University of Phermacy and Applied Life Sciences

    2010 - 2011

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    We screened potential telomerase inhibitors in food stuffs, and found that β-carotene, the most common carotenoid, significantly inhibited telomerase in cell-culture study. After treatment of the cells with β-carotene and vitamin E(α-tocopherol), telomerase activity was synergistically reduced. Furthermore, we ascertained the anti-telomerase action of β-carotene using in vivo tumor models.

  14. Anti-tumor effect of functional foods via telomerase inhibition.

    EITSUKA Takahiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Nigata University of Phermacy and Applied Life Sciences

    2007 - 2008

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Other 3

  1. 米ぬか成分によるエピジェネティック制御を介した癌抑制作用に関する研究

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    米ぬかトコトリエノールによるエピジェネティックな制御機構を介したがん抑制研究

  2. 米ぬか成分によるテロメア短縮抑制を介した細胞老化の防止

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    米ぬかに含まれる各種機能性成分を活用したアンチエイジング研究

  3. 米ぬかトコトリエノールと他成分による相乗的生理作用向上の機構解明

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    米ぬかのトコトリエノールとその他の成分を組み合わせた相乗的な癌抑制作用