TOHOKU UNIVERSITY Researchers - Shinichiro Nomura

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Shinichiro Nomura

Section

Graduate School of Engineering

Job title

Associate Professor

Degree

博士（理学）（京都大学）
修士（学術）（名古屋大学）

researchmap

https://researchmap.jp/SMNomura

J-GLOBAL ID

201101084507740982

Profile

日本学術振興会研究員（DC2, PD），21世紀COE「歯と骨の分子破壊と再構築」特任講師，京都大学WPI物質-細胞統合システム拠点iCeMS特定研究員，JSTさきがけ「界面の構造と機能」兼任などを経て2011年より現職．UWE visiting scholar (2019-2021)．

Research History 6

2011/04 - Present

Tohoku University　Graduate School of Engineering Department of Robotics　Associate Professor
2008/10 - 2012/03

JST　研究員
2008/04 - 2011/03

京都大学WPI 物質-細胞統合システム拠点(iCeMS)　WPI特定研究員
2003/10 - 2008/03

東京医科歯科大学 21世紀COE「歯と骨の分子破壊と再構築」　拠点形成特任講師
2002/04 - 2003/09

東京医科歯科大学生体材料工学研究所　日本学術振興会特別研究員（PD)
2001/04 - 2002/03

京都大学大学院理学研究科　日本学術振興会特別研究員(PD)

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Education 3

Kyoto University　Graduate School, Division of Natural Science　物理学・宇宙物理学

- 2002/03
Nagoya University　Graduate School, Division of Human Informatics

- 1999/03
静岡大学工学部材料精密化学科

1993/04 - 1997/03

Committee Memberships 4

International Confrerence on Manipulation, Automation and Robotics at Small Scales　Program Committee

2018/07 - Present
BIOMOD | International Biomolecular Design Competition　Judge

2011/09 - Present
生物物理学会　分野別専門委員

2008/03 - Present
iGEM 2011 Regional Jamboree – Asia Organizing Committee　Judge

2011/10 -

Professional Memberships 9

人工知能学会ナチュラルコンピューティング研究会
バイオマテリアル学会
バイオ・高分子研究会
日本機械学会
高分子学会
日本ロボット学会
分子ロボティクス研究会
「細胞を創る」研究会
日本生物物理学会

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Research Interests 3

Applied Biophysics
Molecular Robotics
Artificial Cells

Research Areas 4

Nanotechnology/Materials / Biochemistry /
Nanotechnology/Materials / Nanobioscience /
Nanotechnology/Materials / Nanomaterials /
Natural sciences / Bio-, chemical, and soft-matter physics /

Awards 3

2006 International Symposium on Micro-Nanomechatronics and Human Science (MHS2006) Best Paper Award

2006/11　IEEE
東京医科歯科大学生体材料工学研究所第3回 IBB Biofuture Research Encouragement Prize 若手研究者部門最優秀賞

2005/03　東京医科歯科大学生体材料工学研究所
第39回科学技術映像祭科学技術庁長官賞

1998/02　科学技術庁

Papers 121

Autonomous assembly and disassembly of gliding molecular robots regulated by a DNA-based molecular controller Peer-reviewed

Ibuki Kawamata, Kohei Nishiyama, Daiki Matsumoto, Shosei Ichiseki, Jakia J. Keya, Kohei Okuyama, Masatoshi Ichikawa, Arif Md. Rashedul Kabir, Yusuke Sato, Daisuke Inoue, Satoshi Murata, Kazuki Sada, Akira Kakugo, Shin-ichiro M. Nomura

Science Advances　10　(22)　2024/05/31
Publisher: American Association for the Advancement of Science (AAAS)
DOI： 10.1126/sciadv.adn4490 　

eISSN： 2375-2548

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In recent years, there has been a growing interest in engineering dynamic and autonomous systems with robotic functionalities using biomolecules. Specifically, the ability of molecular motors to convert chemical energy to mechanical forces and the programmability of DNA are regarded as promising components for these systems. However, current systems rely on the manual addition of external stimuli, limiting the potential for autonomous molecular systems. Here, we show that DNA-based cascade reactions can act as a molecular controller that drives the autonomous assembly and disassembly of DNA-functionalized microtubules propelled by kinesins. The DNA controller is designed to produce two different DNA strands that program the interaction between the microtubules. The gliding microtubules integrated with the controller autonomously assemble to bundle-like structures and disassemble into discrete filaments without external stimuli, which is observable by fluorescence microscopy. We believe this approach to be a starting point toward more autonomous behavior of motor protein–based multicomponent systems with robotic functionalities.
Scalable Synthesis of Planar Macroscopic Lipid-Based Multi-Compartment Structures. International-journal Peer-reviewed

Richard J Archer, Shogo Hamada, Ryo Shimizu, Shin-Ichiro M Nomura

Langmuir : the ACS journal of surfaces and colloids　2023/03/27

DOI： 10.1021/acs.langmuir.2c02859 　

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As life evolved, the path from simple single cell organisms to multicellular enabled increasingly complex functionalities. The spatial separation of reactions at the micron scale achieved by cellular structures allowed diverse and scalable implementation in biomolecular systems. Mimicking such spatially separated domains in a scalable approach could open a route to creating synthetic cell-like structured systems. Here, we report a facile and scalable method to create multicellular-like, multi-compartment (MC) structures. Aqueous droplet-based compartments ranging from 50 to 400 μm were stabilized and connected together by hydrophobic layers composed of phospholipids and an emulsifier. Planar centimeter-scale MC structures were formed by droplet deposition on a water interface. Further, the resulting macroscopic shapes were shown to be achieved by spatially controlled deposition. To demonstrate configurability and potential versatility, MC assemblies of both homogeneous and mixed compartment types were shown. Notably, magnetically heterogeneous systems were achieved by the inclusion of magnetic nanoparticles in defined sections. Such structures demonstrated actuated motion with structurally imparted directionality. These novel and functionalized structures exemplify a route toward future applications including compartmentally assembled "multicellular" molecular robots.
Spontaneous and driven growth of multicellular lipid compartments to millimeter size from porous polymer structures International-journal International-coauthorship Peer-reviewed

Shin-ichiro M. Nomura, Ryo Shimizu, Richard James Archer, Gen Hayase, Taro Toyota, Richard Mayne, Andrew Adamatzky

ChemSystemChem　2022/04/19
Publisher: Wiley
DOI： 10.1002/syst.202200006 　 10.26434/chemrxiv.12129114.v1 　

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This report describes a method to obtain multicellular shaped compartments made by lipids growing from a sponge-like porous structure. Each compartment is several tens of micrometers in diameter and separated by membranes comprised of phospholipid and amphipathic molecules. The multi-compartment structure spontaneously grew to a millimeter scale, driven by an ionic concentration difference between the interior and exterior environments of the sponge. These compartments can also easily incorporate hydrophilic species as a well as smaller materials such as liposomes. Additionally, we showed that mechanical squeezing of the sponge was also effective in producing multicellular bodies. These simple methods to obtain large-scale multicellular compartment of lipid membrane will help future designs and trials of chemical communications on artificial cells.
Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles Peer-reviewed

Yusuke Sato, Ken Komiya, Ibuki Kawamata, Satoshi Murata, Shin-ichiro M. Nomura

CHEMICAL COMMUNICATIONS　55　(62)　9084-9087　2019/08

DOI： 10.1039/c9cc03277k 　

ISSN： 1359-7345

eISSN： 1364-548X
Intra- and Interbead Communications by an Anchored DNA Structure and Cascaded DNA Reactions

Ibuki Kawamata, Satoru Yoshizawa, Keita Abe, Masahiro Takinoue, Shin-Ichiro M. Nomura, Satoshi Murata

ACS Synthetic Biology　2025/03/14
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acssynbio.4c00709 　

ISSN： 2161-5063

eISSN： 2161-5063
Menthyl acetate powered self-propelled Janus sponge Marangoni motors with self-maintaining surface tension gradients and active mixing Peer-reviewed

Richard J. Archer, Stephen J. Ebbens, Yujin Kubodera, Muneyuki Matsuo, Shin-Ichiro M. Nomura

Journal of Colloid and Interface Science　678　11-19　2025/01
Publisher: Elsevier BV
DOI： 10.1016/j.jcis.2024.08.213 　

ISSN： 0021-9797
A unified purification method for actin-binding proteins using a TEV-cleavable His-Strep-tag Peer-reviewed

Daichi Nakajima, Nozomi Takahashi, Takanari Inoue, Shin-ichiro M. Nomura, Hideaki T. Matsubayashi

MethodsX　13　102884-102884　2024/12
Publisher: Elsevier BV
DOI： 10.1016/j.mex.2024.102884 　

ISSN： 2215-0161
Light-guided actin polymerization drives directed motility in protocells International-coauthorship

Hideaki T. Matsubayashi, Shiva Razavi, T. Willow Rock, Daichi Nakajima, Hideki Nakamura, Daniel A. Kramer, Tomoaki Matsuura, Baoyu Chen, Satoshi Murata, Shinichiro M. Nomura, Takanari Inoue

BioRxiv　2024/10/15

DOI： 10.1101/2024.10.14.617543 　
Transmembrane DNA Sequence Signaling via Hybridization

Kai Yoshida, Keita Abe, Yusuke Sato, Ibuki Kawamata, Richard James Archer, Hideaki T. Matsubayashi, Shogo Hamada, Satoshi Murata, Shin-ichiro Nomura

2024/07/16

DOI： 10.26434/chemrxiv-2024-571kp 　
Primordial aqueous alteration recorded in water-soluble organic molecules from the carbonaceous asteroid (162173) Ryugu Peer-reviewed

Yoshinori Takano, Hiroshi Naraoka, Jason P. Dworkin, Toshiki Koga, Kazunori Sasaki, Hajime Sato, Yasuhiro Oba, Nanako O. Ogawa, Toshihiro Yoshimura, Kenji Hamase, Naohiko Ohkouchi, Eric T. Parker, José C. Aponte, Daniel P. Glavin, Yoshihiro Furukawa, Junken Aoki, Kuniyuki Kano, Shin-ichiro M. Nomura, Francois-Regis Orthous-Daunay, Philippe Schmitt-Kopplin, Aogu Furusho, Minako Hashiguchi, Kazuhiko Fukushima, Dan Aoki, Hannah L. McLain, Jamie E. Elsila, Heather V. Graham, John M. Eiler, Norbert Hertkorn, Alexander Ruf, Cédric Wolters, Junko Isa, Véronique Vuitton, Roland Thissen, Saburo Sakai, Haruna Sugahara, Hajime Mita, Yoshito Chikaraishi, Takaaki Yoshikawa, Satoru Tanaka, Mayu Morita, Morihiko Onose, Daisuke Araoka, Fumie Kabashima, Kosuke Fujishima, Hajime Sato, Tomoya Yamazaki, Yuki Kimura, Hisayoshi Yurimoto, Tomoki Nakamura, Takaaki Noguchi, Ryuji Okazaki, Hikaru Yabuta, Kanako Sakamoto, Toru Yada, Masahiro Nishimura, Aiko Nakato, Akiko Miyazaki, Kasumi Yogata, Masanao Abe, Tatsuaki Okada, Tomohiro Usui, Makoto Yoshikawa, Takanao Saiki, Satoshi Tanaka, Fuyuto Terui, Satoru Nakazawa, Sei-ichiro Watanabe, Yuichi Tsuda, Shogo Tachibana

Nature Communications　15　(1)　2024/07/10
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41467-024-49237-6 　

eISSN： 2041-1723

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Abstract We report primordial aqueous alteration signatures in water-soluble organic molecules from the carbonaceous asteroid (162173) Ryugu by the Hayabusa2 spacecraft of JAXA. Newly identified low-molecular-weight hydroxy acids (HO-R-COOH) and dicarboxylic acids (HOOC-R-COOH), such as glycolic acid, lactic acid, glyceric acid, oxalic acid, and succinic acid, are predominant in samples from the two touchdown locations at Ryugu. The quantitative and qualitative profiles for the hydrophilic molecules between the two sampling locations shows similar trends within the order of ppb (parts per billion) to ppm (parts per million). A wide variety of structural isomers, including α- and β-hydroxy acids, are observed among the hydrophilic molecules. We also identify pyruvic acid and dihydroxy and tricarboxylic acids, which are biochemically important intermediates relevant to molecular evolution, such as the primordial TCA (tricarboxylic acid) cycle. Here, we find evidence that the asteroid Ryugu samples underwent substantial aqueous alteration, as revealed by the presence of malonic acid during keto–enol tautomerism in the dicarboxylic acid profile. The comprehensive data suggest the presence of a series for water-soluble organic molecules in the regolith of Ryugu and evidence of signatures in coevolutionary aqueous alteration between water and organics in this carbonaceous asteroid.
Programmable Computational RNA Droplets Assembled via Kissing-Loop Interaction Peer-reviewed

Hirotake Udono, Minzhi Fan, Yoko Saito, Hirohisa Ohno, Shin-ichiro M. Nomura, Yoshihiro Shimizu, Hirohide Saito, Masahiro Takinoue

ACS Nano　18　(24)　15477-15486　2024/06/04
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acsnano.3c12161 　

ISSN： 1936-0851

eISSN： 1936-086X
Enantioselective three-dimensional high-performance liquid chromatographic determination of amino acids in the Hayabusa2 returned samples from the asteroid Ryugu

Aogu Furusho, Chiharu Ishii, Takeyuki Akita, Mai Oyaide, Masashi Mita, Hiroshi Naraoka, Yoshinori Takano, Jason P. Dworkin, Yasuhiro Oba, Toshiki Koga, Kazuhiko Fukushima, Dan Aoki, Minako Hashiguchi, Hajime Mita, Yoshito Chikaraishi, Naohiko Ohkouchi, Nanako O. Ogawa, Saburo Sakai, Daniel P. Glavin, Jamie E. Elsila, Eric T. Parker, José C. Aponte, Hannah L. McLain, Francois-Regis Orthous-Daunay, Véronique Vuitton, Roland Thissen, Cédric Wolters, Philippe Schmitt-Kopplin, Alexander Ruf, Junko Isa, Norbert Hertkorn, John M. Eiler, Toshihiro Yoshimura, Haruna Sugahara, Heather V. Graham, Yoshihiro Furukawa, Daisuke Araoka, Satoru Tanaka, Takaaki Yoshikawa, Fumie Kabashima, Kazunori Sasaki, Hajime Sato, Tomoya Yamazaki, Morihiko Onose, Mayu Morita, Yuki Kimura, Kuniyuki Kano, Junken Aoki, Kosuke Fujishima, Shin-ichiro Nomura, Shogo Tachibana

Journal of Chromatography Open　2024/05

DOI： 10.1016/j.jcoa.2024.100134 　

ISSN： 2772-3917
Liquid DNA Coacervates form Porous Capsular Hydrogels via Viscoelastic Phase Separation on Microdroplet Interface Peer-reviewed

Masamune Morita, Tetsuro Sakamoto, Shin‐ichiro M. Nomura, Satoshi Murata, Miho Yanagisawa, Masahiro Takinoue

Advanced Materials Interfaces　2024/05

DOI： 10.1002/admi.202300898 　
Polycyclic aromatic hydrocarbons in samples of Ryugu formed in the interstellar medium Peer-reviewed

Sarah S. Zeichner, José C. Aponte, Surjyendu Bhattacharjee, Guannan Dong, Amy E. Hofmann, Jason P. Dworkin, Daniel P. Glavin, Jamie E. Elsila, Heather V. Graham, Hiroshi Naraoka, Yoshinori Takano, Shogo Tachibana, Allison T. Karp, Kliti Grice, Alex I. Holman, Katherine H. Freeman, Hisayoshi Yurimoto, Tomoki Nakamura, Takaaki Noguchi, Ryuji Okazaki, Hikaru Yabuta, Kanako Sakamoto, Toru Yada, Masahiro Nishimura, Aiko Nakato, Akiko Miyazaki, Kasumi Yogata, Masanao Abe, Tatsuaki Okada, Tomohiro Usui, Makoto Yoshikawa, Takanao Saiki, Satoshi Tanaka, Fuyuto Terui, Satoru Nakazawa, Sei-ichiro Watanabe, Yuichi Tsuda, Kenji Hamase, Kazuhiko Fukushima, Dan Aoki, Minako Hashiguchi, Hajime Mita, Yoshito Chikaraishi, Naohiko Ohkouchi, Nanako O. Ogawa, Saburo Sakai, Eric T. Parker, Hannah L. McLain, Francois-Regis Orthous-Daunay, Véronique Vuitton, Cédric Wolters, Philippe Schmitt-Kopplin, Norbert Hertkorn, Roland Thissen, Alexander Ruf, Junko Isa, Yasuhiro Oba, Toshiki Koga, Toshihiro Yoshimura, Daisuke Araoka, Haruna Sugahara, Aogu Furusho, Yoshihiro Furukawa, Junken Aoki, Kuniyuki Kano, Shin-ichiro M. Nomura, Kazunori Sasaki, Hajime Sato, Takaaki Yoshikawa, Satoru Tanaka, Mayu Morita, Morihiko Onose, Fumie Kabashima, Kosuke Fujishima, Tomoya Yamazaki, Yuki Kimura, John M. Eiler

Science　382　(6677)　1411-1416　2023/12/22
Publisher: American Association for the Advancement of Science (AAAS)
DOI： 10.1126/science.adg6304 　

ISSN： 0036-8075

eISSN： 1095-9203

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Polycyclic aromatic hydrocarbons (PAHs) contain ≲20% of the carbon in the interstellar medium. They are potentially produced in circumstellar environments (at temperatures ≳1000 kelvin), by (~10 kelvin) interstellar clouds, or by processing of carbon-rich dust grains. We report isotopic properties of PAHs extracted from samples of the asteroid Ryugu and the meteorite Murchison. The doubly- ¹³ C substituted compositions (Δ2× ¹³ C values) of the PAHs naphthalene, fluoranthene, and pyrene are 9 to 51‰ higher than values expected for a stochastic distribution of isotopes. The Δ2× ¹³ C values are higher than expected if the PAHs formed in a circumstellar environment, but consistent with formation in the interstellar medium. By contrast, the PAHs phenanthrene and anthracene in Ryugu samples have Δ2× ¹³ C values consistent with formation by higher-temperature reactions.
Chemical evolution of primordial salts and organic sulfur molecules in the asteroid 162173 Ryugu

Toshihiro Yoshimura, Yoshinori Takano, Hiroshi Naraoka, Toshiki Koga, Daisuke Araoka, Nanako O. Ogawa, Philippe Schmitt-Kopplin, Norbert Hertkorn, Yasuhiro Oba, Jason P. Dworkin, José C. Aponte, Takaaki Yoshikawa, Satoru Tanaka, Naohiko Ohkouchi, Minako Hashiguchi, Hannah McLain, Eric T. Parker, Saburo Sakai, Mihoko Yamaguchi, Takahiro Suzuki, Tetsuya Yokoyama, Hisayoshi Yurimoto, Tomoki Nakamura, Takaaki Noguchi, Ryuji Okazaki, Hikaru Yabuta, Kanako Sakamoto, Toru Yada, Masahiro Nishimura, Aiko Nakato, Akiko Miyazaki, Kasumi Yogata, Masanao Abe, Tatsuaki Okada, Tomohiro Usui, Makoto Yoshikawa, Takanao Saiki, Satoshi Tanaka, Fuyuto Terui, Satoru Nakazawa, Sei-ichiro Watanabe, Yuichi Tsuda, Shogo Tachibana, Kenji Hamase, Aogu Furusho, Kazuhiko Fukushima, Dan Aoki, Daniel P. Glavin, Hannah L. McLain, Jamie E. Elsila, Heather V. Graham, John M. Eiler, Alexander Ruf, Francois-Regis Orthous-Daunay, Cédric Wolters, Junko Isa, Véronique Vuitton, Roland Thissen, Haruna Sugahara, Hajime Mita, Yoshihiro Furukawa, Yoshito Chikaraishi, Mayu Morita, Morihiko Onose, Fumie Kabashima, Kosuke Fujishima, Hajime Sato, Kazunori Sasaki, Kuniyuki Kano, Shin-ichiro M. Nomura, Junken Aoki, Tomoya Yamazaki, Yuki Kimura

Nature Communications　14　(1)　2023/09/18
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41467-023-40871-0 　

eISSN： 2041-1723

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Abstract Samples from the carbonaceous asteroid (162173) Ryugu provide information on the chemical evolution of organic molecules in the early solar system. Here we show the element partitioning of the major component ions by sequential extractions of salts, carbonates, and phyllosilicate-bearing fractions to reveal primordial brine composition of the primitive asteroid. Sodium is the dominant electrolyte of the salt fraction extract. Anions and NH₄⁺ are more abundant in the salt fraction than in the carbonate and phyllosilicate fractions, with molar concentrations in the order SO₄²⁻> Cl⁻> S₂O₃²⁻> NO₃⁻> NH₄⁺. The salt fraction extracts contain anionic soluble sulfur-bearing species such as S_n-polythionic acids (n < 6), C_n-alkylsulfonates, alkylthiosulfonates, hydroxyalkylsulfonates, and hydroxyalkylthiosulfonates (n < 7). The sulfur-bearing soluble compounds may have driven the molecular evolution of prebiotic organic material transforming simple organic molecules into hydrophilic, amphiphilic, and refractory S allotropes.
[Review] From Molecular Robotics to Molecular Cybernetics: The First Step Toward Chemical Artificial Intelligence Invited

Akinori Kuzuya, Shin-Ichiro M. Nomura, Taro Toyota, Takashi Nakakuki, Satoshi Murata

IEEE Transactions on Molecular, Biological, and Multi-Scale Communications　2023

DOI： 10.1109/TMBMC.2023.3304243 　
Surfactant‐Assisted Purification of Hydrophobic DNA Nanostructures

Shoji Iwabuchi, Shin-ichiro M Nomura, Yusuke Sato

ChemBioChem　2022/12/05
Publisher: Wiley
DOI： 10.1002/cbic.202200568 　

ISSN： 1439-4227

eISSN： 1439-7633
Autonomous and Programmable Strand Generator Implemented as DNA and Enzymatic Chemical Reaction Cascade

Ibuki Kawamata, Shin-ichiro M. Nomura, Satoshi Murata

New Generation Computing　40　(2)　723-736　2022/02/27
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s00354-022-00156-4 　

ISSN： 0288-3635

eISSN： 1882-7055
[Review] Molecular Cybernetics: Challenges toward Cellular Chemical Artificial Intelligence Peer-reviewed

S. Murata, T. Toyota, S.-i. M. Nomura, T. Nakakuki, A. Kuzuya

Advanced Functional Materials　2201866　2022

DOI： 10.1002/adfm.202201866 　
Construction of Artificial Cell-type Molecular Robots

Shoji IWABUCHI, Noriki FUKAMI, Yusuke SATO, Shin-ichiro M. NOMURA

Seibutsu Butsuri　62　(3)　178-180　2022
Publisher: Biophysical Society of Japan
DOI： 10.2142/biophys.62.178 　

ISSN： 0582-4052

eISSN： 1347-4219
System concentration shift as a regulator of transcription-translation system within liposomes. International-journal

Toshiki Akui, Kei Fujiwara, Gaku Sato, Masahiro Takinoue, Shin-Ichiro M Nomura, Nobuhide Doi

iScience　24　(8)　102859-102859　2021/08/20

DOI： 10.1016/j.isci.2021.102859 　

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Biochemical systems in living cells have their optimum concentration ratio among each constituent element to maintain their functionality. However, in the case of the biochemical system with complex interactions and feedbacks among elements, their activity as a system greatly changes by the concentration shift of the entire system irrespective of the concentration ratio among elements. In this study, by using a transcription-translation (TX-TL) system as the subject, we illustrate the principle of the nonlinear relationship between the system concentration and the activity of the system. Our experiment and simulation showed that shifts of the system concentration of TX-TL by dilution and concentration works as a switch of activity and demonstrated its ability to induce a biochemical system to confer the permeability of small molecules to liposomes. These results contribute to the creation of artificial cells with the switch and provide an insight into the emergence of protocells.
Online Biomolecular Design Competition Across University Boundaries

Satoshi MURATA, Akinori KUZUYA, Kei FUJIWARA, Junichi TAIRA, Ibuki KAWAMATA, Yusuke SATO, Masahiro TAKINOUE, Shin-ichiro M. NOMURA, Akira KAKUGO, Yutaka HORI, Keita ABE

Journal of JSEE　69　(4)　4_31-4_39　2021
Publisher: Japanese Society for Engineering Education
DOI： 10.4307/jsee.69.4_31 　

ISSN： 1341-2167

eISSN： 1881-0764
A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane Peer-reviewed

Shoji Iwabuchi, Ibuki Kawamata, Satoshi Murata, Shin-ichiro M. Nomura

Chemical Communications　57　(24)　2990-2993　2021
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d0cc07412h 　

ISSN： 1359-7345

eISSN： 1364-548X

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<p>DNA origami nanopore with large size of a 10 nm square, equipping a tunable lid, enables size-selective molecular transportation through the lipid membrane of giant vesicle.</p>
Formation of DNA micro-skeleton structures in water-in-oil microdroplets

Masamune Morita, Shin-Ichiro M. Nomura, Satoshi Murata, Miho Yanagisawa, Masahiro Takinoue

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017　159-160　2020
Publisher: Chemical and Biological Microsystems Society
DNA Origami "Quick" Refolding inside of a Micron-Sized Compartment Peer-reviewed

Taiki Watanabe, Yusuke Sato, Hayato Otaka, Ibuki Kawamata, Satoshi Murata, Shin-Ichiro M. Nomura

MOLECULES　25　(1)　2020/01

DOI： 10.3390/molecules25010008 　

eISSN： 1420-3049
Fabrication of refractive index sensors using polarization independent metamaterial and their application to chemical concentration measurement and DNA detection Peer-reviewed

Kanamori Yoshiaki, Shimizu Tomoki, Nomura Shin-Ichiro, Hane Kazuhiro

ELECTRONICS AND COMMUNICATIONS IN JAPAN　2019/08/13

DOI： 10.1002/ecj.12208 　

ISSN： 1942-9533
Macroporous Silicone Sheets Integrated with Meshes for Various Applications Peer-reviewed

Hayase Gen, Nomura Shin-ichiro M

ACS APPLIED POLYMER MATERIALS　1　(8)　2077-2082　2019/08

DOI： 10.1021/acsapm.9b00382 　
Pattern Formation on Discrete Gel Matrix Based on DNA Computing Peer-reviewed

Hosoya, T., Kawamata, I., Nomura, S.-I.M., Murata, S.

New Generation Computing　37　(1)　97-111　2019

DOI： 10.1007/s00354-018-0047-1 　

ISSN： 0288-3635
Fabrication of Refractive Index Sensors using Polarization Independent Metamaterial and Their Application to Chemical Concentration Measurement and DNA Detection,偏光無依存メタマテリアルを用いた屈折率センサの製作と化学物質濃度計測及び DNA 検出への適用 Peer-reviewed

Kanamori, Y., Shimizu, T., Nomura, S.-I., Hane, K.

IEEJ Transactions on Sensors and Micromachines　139　(7)　163-168　2019

DOI： 10.1541/ieejsmas.139.163 　

ISSN： 1341-8939
Programmable reactions and diffusion using DNA for pattern formation in hydrogel medium Peer-reviewed

Abe, K., Kawamata, I., Nomura, S.-I.M., Murata, S.

Molecular Systems Design and Engineering　4　(3)　639-643　2019

DOI： 10.1039/c9me00004f 　
Environment-Dependent Self-Assembly of DNA Origami Lattices on Phase-Separated Lipid Membranes Peer-reviewed

Yusuke Sato, Masayuki Endo, Masamune Morita, Masahiro Takinoue, Hiroshi Sugiyama, Satoshi Murata, Shin-ichiro M. Nomura, Yuki Suzuki

ADVANCED MATERIALS INTERFACES　5　(14)　2018/07

DOI： 10.1002/admi.201800437 　

ISSN： 2196-7350
Diffusion modulation of DNA by toehold exchange Peer-reviewed

Thanapop Rodjanapanyakul, Fumi Takabatake, Keita Abe, Ibuki Kawamata, Shinichiro M. Nomura, Satoshi Murata

Physical Review E　97　(5)　2018/05/31
Publisher: American Physical Society
DOI： 10.1103/PhysRevE.97.052617 　

ISSN： 2470-0053 2470-0045
Large-Scale Preparation of Giant Vesicles by Squeezing a Lipid-Coated Marshmallow-like Silicone Gel in a Buffer Peer-reviewed

Hayase, G., Nomura, S.-I.M.

Langmuir　34　(37)　11021-11026　2018

DOI： 10.1021/acs.langmuir.8b01801 　

ISSN： 0743-7463
Construction of T-Motif-Based DNA Nanostructures through Enzymatic Reactions International-journal Peer-reviewed

Kageyama, R., Kawamata, I., Tanabe, K., Suzuki, Y., Nomura, S.-I.M., Murata, S.

ChemBioChem　19　(8)　873-876　2018

DOI： 10.1002/cbic.201700682 　

ISSN： 1439-4227
Revolving Vernier Mechanism Controls Size of Linear Homomultimer Peer-reviewed

Takeo Uchida, Keita Abe, Yuma Endo, Shosei Ichiseki, Satoru Akita, Shiyun Liu, Sho Aradachi, Masataka Saito, Akihiko Fukuchi, Taiyo Kikkawa, Theo Dammaretz, Ibuki Kawamata, Yuki Suzuki, Shin-ichiro M. Nomura, Satoshi Murata

SMALL　13　(41)　2017/11

DOI： 10.1002/smll.201702158 　

ISSN： 1613-6810

eISSN： 1613-6829
Stepping operation of a rotary DNA origami device Peer-reviewed

Takahiro Tomaru, Yuki Suzuki, Ibuki Kawamata, Shin-ichiro M. Nomura, Satoshi Murata

CHEMICAL COMMUNICATIONS　53　(55)　7716-7719　2017/07

DOI： 10.1039/c7cc03214e 　

ISSN： 1359-7345

eISSN： 1364-548X
Micrometer-sized molecular robot changes its shape in response to signal molecules Peer-reviewed

Yusuke Sato, Yuichi Hiratsuka, Ibuki Kawamata, Satoshi Murata, Shin-ichiro M. Nomura

SCIENCE ROBOTICS　2　(4)　2017/03

DOI： 10.1126/scirobotics.aal3735 　

ISSN： 2470-9476
Non natural tiny-wet machine: Molecular Robot Peer-reviewed

2017 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)　2017

ISSN： 2474-378X
Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels Peer-reviewed

Cervantes-Salguero, K., Kawamata, I., Nomura, S.-I.M., Murata, S.

Physical Chemistry Chemical Physics　19　(21)　13414-13418　2017

DOI： 10.1039/c7cp02214j 　

ISSN： 1463-9076

eISSN： 1463-9084
In vitro transcription-Translation using bacterial genome as a template to reconstitute intracellular profile Peer-reviewed

Fujiwara, K., Sawamura, T., Niwa, T., Deyama, T., Nomura, S.-I.M., Taguchi, H., Doi, N.

Nucleic Acids Research　45　(19)　11449-11458　2017

DOI： 10.1093/nar/gkx776 　

ISSN： 0305-1048

eISSN： 1362-4962
DNA cytoskeleton for stabilizing artificial cells Peer-reviewed

Kurokawa, C., Fujiwara, K., Morita, M., Kawamata, I., Kawagishi, Y., Sakai, A., Murayama, Y., Nomura, S.-I.M., Murata, S., Takinoue, M., Yanagisawa, M.

Proceedings of the National Academy of Sciences of the United States of America　114　(28)　7228-7233　2017

DOI： 10.1073/pnas.1702208114 　

ISSN： 0027-8424
DNA integral controller learning from biological system Peer-reviewed

Yuki Tanaka, Takashi Nakakuki, Shin-ichiro M. Nomura

2017 56TH ANNUAL CONFERENCE OF THE SOCIETY OF INSTRUMENT AND CONTROL ENGINEERS OF JAPAN (SICE)　2017-November　781-786　2017

DOI： 10.23919/SICE.2017.8105607 　
How to Develop Students’ Creativity? ~A Case of Student Competition of Biomolecular Design Invited Peer-reviewed

Satoshi Murata, Ibuki Kawamata, Shin-ichiro M. Nomura

Proceeding of IEEE-ICCSE2016　13-20　2016/08
DNA Switcher for a Molecular Robot Peer-reviewed

Nishijima K, Nakakuki T, Sato Y, Nomura S

Proc. of ICTROBOT　1　2016
Reversible Gel–Sol Transition of a Photo-Responsive DNA Gel Peer-reviewed

Kandatsu, D., Cervantes-Salguero, K., Kawamata, I., Hamada, S., Nomura, S.-I.M., Fujimoto, K., Murata, S.

ChemBioChem　17　(12)　1118-1121　2016

DOI： 10.1002/cbic.201600088 　

ISSN： 1439-4227

eISSN： 1439-7633
Pattern Formation and Computation by Autonomous Chemical Reaction Diffusion Model Inspired by Cellular Automata Peer-reviewed

Ibuki Kawamata, Takuto Hosoya, Fumi Takabatake, Ken Sugawara, Shin-ichiro M. Nomura, Teijiro Isokawa, Ferdinand Peper, Masami Hagiya, Satoshi Murata

2016 FOURTH INTERNATIONAL SYMPOSIUM ON COMPUTING AND NETWORKING (CANDAR)　215-221　2016

DOI： 10.1109/CANDAR.2016.55 　

ISSN： 2379-1888
Trading polymeric microspheres: Exchanging DNA molecules via microsphere interaction Peer-reviewed

Nobuyuki Morimoto, Kanna Muramatsu, Shin-ichiro M. Nomura, Makoto Suzuki

COLLOIDS AND SURFACES B-BIOINTERFACES　128　94-99　2015/04

DOI： 10.1016/j.colsurfb.2015.02.014 　

ISSN： 0927-7765

eISSN： 1873-4367
Polymorphic ring-shaped molecular clusters made of shape-variable building blocks Peer-reviewed

Cervantes-Salguero, K., Hamada, S., Nomura, S.-I.M., Murata, S.

Nanomaterials　5　(1)　208-217　2015

DOI： 10.3390/nano5010208 　

ISSN： 2079-4991
Introducing Micrometer-Sized Artificial Objects into Live Cells: A Method for Cell-Giant Unilamellar Vesicle Electrofusion Peer-reviewed

Akira C. Saito, Toshihiko Ogura, Kei Fujiwara, Satoshi Murata, Shin-ichiro M. Nomura

PLOS ONE　9　(9)　e106853　2014/09

DOI： 10.1371/journal.pone.0106853 　

ISSN： 1932-6203
Temperature-responsive telechelic dipalmitoylglyceryl poly(N-isopropylacrylamide) vesicles: real-time morphology observation in aqueous suspension and in the presence of giant liposomes Peer-reviewed

Nobuyuki Morimoto, Yu Sasaki, Kouki Mitsunushi, Evgeniya Korchagina, Tetsuichi Wazawa, Xing-Ping Qiu, Shin-ichiro M. Nomura, Makoto Suzuki, Francoise M. Winnik

CHEMICAL COMMUNICATIONS　50　(61)　8350-8352　2014/08

DOI： 10.1039/c4cc03199g 　

ISSN： 1359-7345

eISSN： 1364-548X
Temperature-responsive telechelic dipalmitoylglyceryl poly(N-isopropylacrylamide) vesicles: real-time morphology observation in aqueous suspension and in the presence of giant liposomes Peer-reviewed

Nobuyuki Morimoto, Yu Sasaki, Kouki Mitsunushi, Evgeniya Korchagina, Tetsuichi Wazawa, Xing-Ping Qiu, Shin-ichiro M. Nomura, Makoto Suzuki, Francoise M. Winnik

CHEMICAL COMMUNICATIONS　50　(61)　8350-8352　2014/08

DOI： 10.1039/c4cc03199g 　

ISSN： 1359-7345

eISSN： 1364-548X
2P315 Toward creating an autonomous mobile artificial amoeba(28. Bioengineering,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Tanaka Yoshiaki, Hiratsuka Yuichi, Fujiwara Kei, Murata Satoshi, M. Nomura Shinichiro

Seibutsu Butsuri　54　(1)　S247　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S247_3 　
2P179 Introducing micro-meter size objects into live cells mediated by cell-GUV electrofusion(12. Cell biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Saito Akira C., Ogura Toshihiko, Fujiwara Kei, Murata Satoshi, Nomura Shinichiro M.

Seibutsu Butsuri　54　(1)　S224　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S224_5 　
DNAナノテクノロジーのためのアウトリーチツールの開発と評価 Peer-reviewed

吉澤慧, 藤原慶, 野村 M, 慎一郎, 村田智

形の科学会誌　29　(2)　87-96　2014
Reconstitution of intracellular environments in vitro and in artificial cells Peer-reviewed

Kei Fujiwara, Miho Yanagisawa, Shin-ichiro M. Nomura

BIOPHYSICS　10　43-48　2014

DOI： 10.2142/biophysics.10.43. 　
Condensation of Cell Extracts to Mimic Intracellular Condition

FUJIWARA Kei, M.NOMURA Shin-ichiro

Seibutsu Butsuri　53　(5)　262-263　2013/09/25
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.262 　

ISSN： 0582-4052
Construction of a remote-controlled supramolecular micro-crawler Peer-reviewed

D. Komatsu, K. Fujiwara, S.-i. M. Nomura

ADVANCES IN ARTIFICIAL LIFE, ECAL 2013　208-209　2013/09/02

DOI： 10.7551/978-0-262-31709-2-ch031 　
Molecular robotics approach for constructing an artificial cell model Peer-reviewed

S.-i. M. Nomura, Y. Sato, K. Fujiwara

ADVANCES IN ARTIFICIAL LIFE, ECAL 2013　488-491　2013/09/02
Publisher: MIT Press
DOI： 10.7551/978-0-262-31709-2-ch070 　
Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system Peer-reviewed

Kei Fujiwara, Tsutomu Katayama, Shin-Ichiro M. Nomura

Nucleic Acids Research　41　(14)　7176-7183　2013/08

DOI： 10.1093/nar/gkt489 　

ISSN： 0305-1048 1362-4962
2P217 How to send artifacts into the living cell inside? : Investigating GUV-Cell electro fusion method(13B. Biological & Artifical membrane: Dynamics,Poster)

Saito Akira C., Ogura Toshihiko, Nomura Shinichiro M.

Seibutsu Butsuri　53　(1)　S194　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S194_6 　
2P314 Stability of designed high-order DNA structures under unconventional conditions(28. Bioengineering,Poster)

Endo Masahiro, Fujiwara Kei, Murata Satoshi, Nomura Shin-ichiro

Seibutsu Butsuri　53　(1)　S211　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S211_1 　
2P315 Self-assembly and reconfiguration of multiple-sized closed structures made of DNA origami units(28. Bioengineering,Poster)

Cervantes Keitel, Hamada Shogo, Nomura Shin-ichiro, Murata Satoshi

Seibutsu Butsuri　53　(1)　S211　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S211_2 　
2P218 Generation of artificial cells that mimic living cells(13B. Biological & Artifical membrane: Dynamics,Poster)

Fujiwara Kei, Nishizawa Kenji, Yanagisawa Miho, Nomura Shin-ichiro M., Mizuno Daisuke

Seibutsu Butsuri　53　(1)　S195　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S195_1 　
3P124 Design and construction of a DNA microcapsule toward lightresponsive molecular robots(05A. Nucleic acid: Structure & Property,Poster)

Tsuganezawa Yuichi, Morita Masamune, Hamada Shogo, Nomura Shin-ichiro M., Fujimoto Kenzo, Murata Satoshi, Takinoue Masahiro

Seibutsu Butsuri　53　(1)　S232　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S232_3 　
1SBA-02 Molecular robotics approach for constructing an artificial cell model(1SBA The Exploration of the design principle of biomolecular robots based on biophysics,Symposium,The 51th Annual Meeting of the Biophysical Society of Japan)

Nomura M. Shin-ichiro, Fujiwara Kei

Seibutsu Butsuri　53　(1)　S83　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S83_3 　
Condensation of an Additive-Free Cell Extract to Mimic the Conditions of Live Cells Peer-reviewed

Kei Fujiwara, Shin-ichiro M. Nomura

PLOS ONE　8　(1)　e54155　2013/01

DOI： 10.1371/journal.pone.0054155 　

ISSN： 1932-6203
A bacterial salt sensor created by multiplying phenotypes of GroE-depleted Escherichia coli Peer-reviewed

Kei Fujiwara, Keita B. Aoi, Shin-ichiro M. Nomura

ANALYTICAL METHODS　5　(21)　5918-5922　2013

DOI： 10.1039/c3ay41092g 　

ISSN： 1759-9660

eISSN： 1759-9679
1PT226 Artificial Panspermia by Molecular Robots(The 50th Annual Meeting of the Biophysical Society of Japan)

Endo Masahiro, Fujiwara Kei, Hamada Syougo, Murata Satoshi, Nomura Shin-ichiro M

Seibutsu Butsuri　52　S107　2012
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.52.S107_1 　
3PT112 Adsorption of DNA ring onto Liposome(The 50th Annual Meeting of the Biophysical Society of Japan)

Kumagai Yumiko, Hamada Shogo, Nomura Shin-ichiro, Murata Satoshi

Seibutsu Butsuri　52　S159　2012
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.52.S159_4 　
3PT113 Substrate-Assisted Self-Assembly of DNA Nanostructure on lipid bilayer(The 50th Annual Meeting of the Biophysical Society of Japan)

Kudo Shogo, Hamada Shogo, Nomura Shin-ichiro, Murata Satoshi

Seibutsu Butsuri　52　S159　2012
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.52.S159_5 　
Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes Peer-reviewed

Takuma Ohtsuka, Satoshi Neki, Tamotsu Kanai, Kazunari Akiyoshi, Shin-ichiro M. Nomura, Takashi Ohtsuki

ANALYTICAL BIOCHEMISTRY　418　(1)　97-101　2011/11

DOI： 10.1016/j.ab.2011.06.026 　

ISSN： 0003-2697
Differentiating stem cells on patterned substrates for neural network formation

ChunXiong Luo, Li Liu, XiaoFang Ni, Li Wang, Shinichiro M. Nomura, Qi Ouyang, Yong Chen

MICROELECTRONIC ENGINEERING　88　(8)　1707-1710　2011/08

DOI： 10.1016/j.mee.2010.12.062 　

ISSN： 0167-9317
3J1358 A bacterial ion-sensing device without electrodes(Bioengineering2,The 49th Annual Meeting of the Biophysical Society of Japan)

Fujiwara Kei, Aoi Keita, Nomura M. Shin-ichiro

Seibutsu Butsuri　51　S142　2011
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.51.S142_4 　
Molecular Communication between Liposomes and Cells

NOMURA Shin-ichiro, MORITANI Yuki, AKIYOSHI Kazunari

JRSJ　28　(10)　1176-1177　2010/12
Publisher: The Robotics Society of Japan
DOI： 10.7210/jrsj.28.1176 　

ISSN： 0289-1824
Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation Peer-reviewed

Yuki Moritani, Shin-ichiro M. Nomura, Ikuo Morita, Kazunari Akiyoshi

FEBS JOURNAL　277　(16)　3343-3352　2010/08

DOI： 10.1111/j.1742-4658.2010.07736.x 　

ISSN： 1742-464X

eISSN： 1742-4658
A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate Peer-reviewed

Li Liu, Chunxiong Luo, Xiaofeng Ni, Li Wang, Kaori Yamauchi, Shinichi M. Nomura, Norio Nakatsuji, Yong Chen

BIOMEDICAL MICRODEVICES　12　(3)　505-511　2010/06

DOI： 10.1007/s10544-010-9407-4 　

ISSN： 1387-2176
Degassing-Assisted Patterning of Cell Culture Surfaces Peer-reviewed

Chunxiong Luo, Xiaofang Ni, Li Liu, Shin-ichiro M. Nomura, Yong Chen

BIOTECHNOLOGY AND BIOENGINEERING　105　(4)　854-859　2010/03

DOI： 10.1002/bit22586 　

ISSN： 0006-3592
3P232 Phenotypic behaviors of giant liposomes containing bacterial cell extracts and chromosomal DNA(Biol & Artifi memb.: Dynamics,The 48th Annual Meeting of the Biophysical Society of Japan)

Fujiwara Kei, Nomura Shin-ichiro

Seibutsu Butsuri　50　(2)　S186　2010
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.50.S186_1 　
Protein Synthesis in Giant Liposomes Using the In Vitro Translation System of Thermococcus kodakaraensis Peer-reviewed

Kazuaki Yamaji, Tamotsu Kanai, Shin-Ichiro M. Nomura, Kazunari Akiyoshi, Makiko Negishi, Yong Chen, Haruyuki Atomi, Kenichi Yoshikawa, Tadayuki Imanaka

IEEE TRANSACTIONS ON NANOBIOSCIENCE　8　(4)　325-331　2009/12

DOI： 10.1109/TNB.2009.2035278 　

ISSN： 1536-1241

eISSN： 1558-2639
Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes Peer-reviewed

Makoto Kaneda, Shin-ichiro M. Nomura, Shizuko Ichinose, Satoshi Kondo, Ken-ichi Nakahama, Kazunari Akiyoshi, Ikuo Morita

BIOMATERIALS　30　(23-24)　3971-3977　2009/08

DOI： 10.1016/j.biomaterials.2009.04.006 　

ISSN： 0142-9612

eISSN： 1878-5905
Giant liposome sorting/collection device: For individual analysis of artificial cell-models Peer-reviewed

Shin-Ichiro M. Nomura, Li Liu, Yong Chen, Hisataka Maruyama, Fumihito Arai

20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science　620-622　2009

DOI： 10.1109/MHS.2009.5352048 　
Artificial-Cellular Computing Peer-reviewed

Shin-Ichiro M. Nomura, Tsutomu Hamada

INTERNATIONAL JOURNAL OF UNCONVENTIONAL COMPUTING　5　(1)　87-102　2009

ISSN： 1548-7199

eISSN： 1548-7202
Artificial-Cellular Computing Peer-reviewed

S.-I. M. NOMURA, T. HAMADA

Int. Journ. of Unconventional Computing　2008
Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis Peer-reviewed

Shin-ichiro M. Nomura, Satoshi Kondoh, Wakiko Asayama, Akikazu Asada, Shigemichi. Nishikawa, Kazunari Akiyoshi

JOURNAL OF BIOTECHNOLOGY　133　(2)　190-195　2008/01

DOI： 10.1016/j.jbiotec.2007.08.023 　

ISSN： 0168-1656

eISSN： 1873-4863
1P324 Functional Membrane Protein Expression and Integration on Lipid Vesicles(Bioengineering, and new biophysical techniques and instrumentation,Oral Presentations)

NOMURA Shin-ichiro M., MORITANI Yuki, ASAYAMA Wakiko, MORITA Ikuo, AKIYOSHI Kazunari

Seibutsu Butsuri　47　S104　2007
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.47.S104_3 　
1P323 Channel Forming Protein Integrated Vesicle Creation for Molecular Communication(Bioengineering,Poster Presentations)

MORITANI Yuki, NOMURA Shin-ichiro M., HIYAMA Satoshi, SUDA Tatsuya, AKIYOSHI Kazunari

Seibutsu Butsuri　47　S104　2007
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.47.S104_2 　
Lipid-tubular network formation for biochemical reaction Peer-reviewed

Shin-ichiro M. Nomura, Kazunari Akiyoshi

2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE, VOLS 1 AND 2　322-326　2007

DOI： 10.1109/MHS.2007.4420874 　

ISSN： 2474-378X

eISSN： 2474-3798
A Molecular Communication Interface Using Liposomes with Gap Junction Proteins

Yuki Moritani, Shin-ichiro M. Nomura, Satoshi Hiyama, Kazunari Akiyoshi, Tatsuya Suda

2006 1ST BIO-INSPIRED MODELS OF NETWORK, INFORMATION AND COMPUTING SYSTEMS　2006
A molecular communication interface using liposomes with gap junction proteins Peer-reviewed

Yuki Moritani, Shin-ichiro M. Nomura, Satoshi Hiyama, Kazunari Akiyoshi, Tatsuya Suda

2006 FIRST INTERNATIONAL CONFERENCE ON COMMUNICATIONS AND NETWORKING IN CHINA　182-182　2006
Nanogel engineered designs for polymeric drug delivery Peer-reviewed

Nobuyuki Morimoto, Shin-ichiro M. Nomura, Naomi Miyazawa, Kazunari Akiyoshi

POLYMERIC DRUG DELIVERY II: POLYMERIC MATRICES AND DRUG PARTICLE ENGINEERING　924　88-101　2006

ISSN： 0097-6156

eISSN： 1947-5918
Self-organaized tubular-linked liposome network Peer-reviewed

Shin-ichiro M. Nomura, Kazunari Akiyoshi

2006 IEEE INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE　13-+　2006

DOI： 10.1109/MHS.2006.320309 　

ISSN： 2474-378X

eISSN： 2474-3798
Preparation and function of self-organaized lipid nanotubular micro network

Shin Ichiro M Nomura, Shin Ichiro M Nomura, Kazunari Akiyoshi, Kazunari Akiyoshi

Polymer Preprints, Japan　54　4803-4804　2005/12/01
Chemosensitive running droplet Peer-reviewed

Y Sumino, H Kitahata, K Yoshikawa, M Nagayama, SM Nomura, N Magome, Y Mori

PHYSICAL REVIEW E　72　(4)　2005/10

DOI： 10.1103/PhysRevE.72.041603 　

ISSN： 1539-3755

eISSN： 1550-2376
Nanogel-quantum dot hybrid nanoparticles for live cell imaging Peer-reviewed

U Hasegawa, SIM Nomura, SC Kaul, T Hirano, K Akiyoshi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　331　(4)　917-921　2005/06

DOI： 10.1016/j.bbrc.2005.03.228 　

ISSN： 0006-291X
Changes in the morphology of cell-size liposomes in the presence of cholesterol: Formation of neuron-like tubes and liposome networks Peer-reviewed

SM Nomura, Y Mizutani, K Kurita, A Watanabe, K Akiyoshi

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES　1669　(2)　164-169　2005/05

DOI： 10.1016/.j.bbamem.2005.02.005 　

ISSN： 0005-2736
3P318 Artificial cells equipped with cellular connection mechanism

Nomura S.-i.M., Kaneda M., Kondoh S., Nakahama K., Morita I., Akiyoshi K.

Seibutsu Butsuri　45　S283　2005
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.45.S283_2 　
2P253 Local morphological changes of liposome networks

Fukagawa A., Nomura M., Ito K., Akiyoshi K.

Seibutsu Butsuri　45　S183　2005
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.45.S183_1 　
Gene expression within cell-sized lipid vesicles Peer-reviewed

S Nomura, K Tsumoto, T Hamada, K Akiyoshi, Y Nakatani, K Yoshikawa

CHEMBIOCHEM　4　(11)　1172-1175　2003/11

DOI： 10.1002/cbic.200300630 　

ISSN： 1439-4227
Enhanced uptake of giant DNA in cell-sized liposomes Peer-reviewed

Y Sato, SM Nomura, K Yoshikawa

CHEMICAL PHYSICS LETTERS　380　(3-4)　279-285　2003/10

DOI： 10.1016/j.cplett.2003.08.050 　

ISSN： 0009-2614
Optically driven transport into a living cell Peer-reviewed

K Kubo, M Ichikawa, K Yoshikawa, Y Koyama, T Niidome, T Yamaoka, SIM Nomura

APPLIED PHYSICS LETTERS　83　(12)　2468-2470　2003/09

DOI： 10.1063/1.1613356 　

ISSN： 0003-6951
Nonlinear dynamics in a laser field: spontaneous oscillation of mesoscopic soft matter Peer-reviewed

SIM Nomura, H Mayama, K Yoshikawa

CHAOS SOLITONS & FRACTALS　17　(2-3)　419-423　2003/07

DOI： 10.1016/S0960-0779(02)00382-X 　

ISSN： 0960-0779
Gene Expression within Cell-Sized Liposomes

Hamada T., Nomura S.-i.M., Tsumoto K.

Seibutsu Butsuri　43　S206　2003
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.43.S206_2 　
Spontaneous Formation of Giant Liposome Network

Nomura S.-i.M., Motoike I.N., Akiyoshi K.

Seibutsu Butsuri　43　S110　2003
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.43.S110_3 　
Induction of neuron-like tubes and liposome networks by cooperative effect of gangliosides and phospholipids Peer-reviewed

K Akiyoshi, A Itaya, SM Nomura, N Ono, K Yoshikawa

FEBS LETTERS　534　(1-3)　33-38　2003/01

DOI： 10.1016/S0014-5793(02)03743-2 　

ISSN： 0014-5793
Rhythmic bursting in a cluster of microbeads driven by a continuous-wave laser beam Peer-reviewed

N Magome, H Kitahata, M Ichikawa, SIM Nomura, K Yoshikawa

PHYSICAL REVIEW E　65　(4)　2002/04

DOI： 10.1103/PhysRevE.65.045202 　

ISSN： 1539-3755
Autonomous swinging of a lipid tubule under stationary irradiation by a Nd³⁺:YAG laser

Nomura, S.-I.M., Harada, T., Yoshikawa, K.

Physical Review Letters　88　(9)　939031-939034　2002/03/04

ISSN： 0031-9007
Autonomous swinging of a lipid tubule under stationary irradiation by a Nd3+: YAG laser Peer-reviewed

SM Nomura, T Harada, K Yoshikawa

PHYSICAL REVIEW LETTERS　88　(9)　2002/03

DOI： 10.1103/PhysRevLett.88.093903 　

ISSN： 0031-9007
Autonomous swinging of a lipid tubule under stationary irradiation by a Nd3+: YAG laser

SM Nomura, T Harada, K Yoshikawa

PHYSICAL REVIEW LETTERS　88　(9)　4　2002/03

DOI： 10.1103/PhysRevLett.88.093903 　

ISSN： 0031-9007
3R1515 Flow Reactor for Giant Vosicle Zoo

Nomura S.-i. M.

Seibutsu Butsuri　42　(2)　S209　2002
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.42.S209_4 　
2L1330 Spontaneous Formation of Cell sized Lipsome Entrapping DNA

Sato Y., Nomura S.-i. M., Yoshikawa K.

Seibutsu Butsuri　42　(2)　S133　2002
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.42.S133_3 　
2M1615 Formation and Function of microcapsule made from Thermal Heterocomplex Molecules of Amino Acids

Sakurazawa S., Nomura S. M.

Seibutsu Butsuri　42　(2)　S139　2002
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.42.S139_3 　
Rhythmic bursting in a cluster of microbeads driven by a continuous-wave laser beam Peer-reviewed

Nobuyuki Magome, Hiroyuki Kitahata, Masatoshi Ichikawa, Shin-ichiro M. Nomura, Kenichi Yoshikawa

Physical Review E - Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics　65　(4)　4　2002

DOI： 10.1103/PhysRevE.65.045202 　

ISSN： 1063-651X
Towards proto-cells: "Primitive" lipid vesicles encapsulating giant DNA and its histone complex

SIM Nomura, K Tsumoto, K Yoshikawa, G Ourisson, Y Nakatani

CELLULAR & MOLECULAR BIOLOGY LETTERS　7　(2)　245-246　2002

ISSN： 1425-8153
Giant liposome as a biochemical reactor: Transcription of DNA and transportation by laser tweezers Peer-reviewed

K Tsumoto, SM Nomura, Y Nakatani, K Yoshikawa

LANGMUIR　17　(23)　7225-7228　2001/11

DOI： 10.1021/la010887s 　

ISSN： 0743-7463
Towards proto-cells: "Primitive" lipid vesicles encapsulating giant DNA and its histone complex Peer-reviewed

SM Nomura, Y Yoshikawa, K Yoshikawa, O Dannenmuller, S Chasserot-Golaz, G Ourisson, Y Nakatani

CHEMBIOCHEM　2　(6)　457-459　2001/06

DOI： 10.1002/1439-7633(20010601)2:6<457::AID-CBIC457>3.3.CO;2-6 　

ISSN： 1439-4227
Self-oscillating polymer chain Peer-reviewed

H Mayama, SM Nomura, H Oana, K Yoshikawa

CHEMICAL PHYSICS LETTERS　330　(3-4)　361-367　2000/11

DOI： 10.1016/S0009-2614(00)01076-9 　

ISSN： 0009-2614
Controlling the folding/unfolding transition of the DNA-histone H1 complex by direct optical manipulation

Y Yoshikawa, SM Nomura, T Kanbe, K Yoshikawa

CHEMICAL PHYSICS LETTERS　330　(1-2)　77-82　2000/11

DOI： 10.1016/S0009-2614(00)01075-7 　

ISSN： 0009-2614
Rhythmic Folding/Unfolding Transition in a Single Polymer Chain under Thermodynamically Open Condition

Nomura S.M., Mayama H.

Seibutsu Butsuri　40　S101　2000
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.40.S101_3 　

ISSN： 0582-4052
Controlling the folding/unfolding transition of the DNA-histon H1 complex by direct optical manipulation Peer-reviewed

Y. Yoshikawa, S.-i. M. Nomura, T. Kanbe, K. Yoshikawa

Chem. Phys. Lett.　2000

DOI： 10.1016/S0009-2614(00)01075-7 　
Giant Phospholipid Vesicles Entrapping Giant DNA Peer-reviewed

S.-i. Nomura, K. Yoshikawa

Giant Vesicles　2000
Laser induced Phospholipid-Tube Oscillation.

Nomura S.

Seibutsu Butsuri　39　S96　1999
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.39.S96_1 　

ISSN： 0582-4052

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Misc. 43

Size Controllable Linear Self-Assembly Module of DNA Origami

LIU Shiyun, 安部桂太, 内田健央, 遠藤佑真, 秋田賢, 市堰翔成, 荒舘笙, 斎藤正崇, 吉川太陽, 福地成彦, 川又生吹, 野村M.慎一郎, 村田智

形の科学シンポジウム講演予稿集(Web)　85th　23‐24 (WEB ONLY)　2018/06

ISSN： 2433-6173
Pattern formation using DNA reaction diffusion system in hydrogel field

安部桂太, 川又生吹, 鈴木勇輝, 野村M, 慎一郎, 村田智

形の科学シンポジウム講演予稿集(Web)　85th　39‐40 (WEB ONLY)　2018/06

ISSN： 2433-6173
Making pores on membrane using DNA origami: Design and function evaluation of .PHI.12 nm artificial channel

岩渕祥璽, 片山航一郎, 川又生吹, 鈴木勇輝, 村田智, 野村M.慎一郎

形の科学シンポジウム講演予稿集(Web)　85th　11‐12 (WEB ONLY)　2018/06

ISSN： 2433-6173
動け，アメーバ型分子ロボット Invited

佐藤佑介, 野村 M. 慎一郎

高分子　67　(6)　332-333　2018/06
分子ロボティクス：アメーバ型分子ロボットの開発 Invited

佐藤佑介, 野村 M. 慎一郎

生化学　90　(5)　728-733　2018/05
Non natural tiny-wet machine: Molecular robot

Sato Yusuke, Hiratsuka Yuichi, Kawamata Ibuki, Murata Satoshi, Nomura M. Shin-Ichiro

MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science　2018-January　1-3　2018/02/28

DOI： 10.1109/MHS.2017.8305293 　
環境応答能を有するらせん状DNAオリガミナノ構造体の構築

水野康平, 鈴木勇輝, 川又生吹, 野村慎一郎, 村田智

自律分散システム・シンポジウム(CD-ROM)　30th　ROMBUNNO.1A1‐3　2018/01/28
DNA反応拡散系によるゲル媒質中でのパターン形成

安部桂太, 川又生吹, 鈴木勇輝, 野村M, 慎一郎, 村田智

自律分散システム・シンポジウム(CD-ROM)　30th　ROMBUNNO.1A1‐2　2018/01/28
Generation of complex self-assembled DNA microstructures inside water-in-oil microdroplets

M. Morita, S. -I. M. Nomura, S. Murata, M. Yanagisawa, M. Takinoue

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS　46　S307-S307　2017/07

ISSN： 0175-7571

eISSN： 1432-1017
膜アンカー型温度応答性高分子が誘起する巨大リポソームの凝集と融合

飛田航, 森本展之, 川又生吹, 村田智, 野村M.慎一郎

高分子学会予稿集(CD-ROM)　66　(1)　ROMBUNNO.3Pd112　2017/05/15
アメーバ型分子ロボットの可能性 Invited

佐藤佑介, 川又生吹, 野村慎一郎

医療と検査機器・試薬　40　(3)　190-194　2017/03
No, friends, I love fictions! Peer-reviewed

Nomura, S.-I.

Kobunshi　66　(5)　228-228　2017

ISSN： 0454-1138
BIOMOD: Road to Molecular Robotics Invited

SATO Yusuke, TAKINOUE Masahiro, NOMURA Shin-ichiro M.

Seibutsu Butsuri　56　(5)　290-292　2016
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.56.290 　

ISSN： 0582-4052
Trading polymeric microspheres: Exchanging DNA molecules via microsphere interaction Peer-reviewed

Morimoto, N., Muramatsu, K., Nomura, S.I.M., Suzuki, M.

Colloids and Surfaces B: Biointerfaces　128　94-99　2015

DOI： 10.1016/j.colsurfb.2015.02.014 　

ISSN： 0927-7765

eISSN： 1873-4367
21pm1-F3 Toward construction an artificial molecular amoeba model

NOMURA M. Shin-ichiro, TANAKA Yoshiaki, HIRATSUKA Yuichi, FUJIWARA Kei, MURATA Satoshi

2014　(6)　"21pm1-F3-1"-"21pm1-F3-2"　2014/10/20
Publisher: The Japan Society of Mechanical Engineers

More details Close

We are aiming at creating molecular robots with controllable mobility. Here we report our attempt to build an autonomous mobile artificial amoeba-like structure, which is consists of linear motor proteins (kinesin/microtubules) and giant liposomes. It is known that liposomal membrane can be transformed by the combination of kinesin and microtubules. Adding ATP to the mixture of kinesin-coated liposomes with microtubules organized an aster-like structure. The bundled microtubules were observed by phase contrast microscopy. After formation of the structures, they started translational motion to random direction. We are planning to utilize the aster as a movable frame or a controllable skeleton of an amoeba-like molecular robot.
Design and Construction of an Artificial Cell Model

NOMURA Shin-ichiro M.

IEICE technical report. ME and bio cybernetics　113　(314)　25-25　2013/11/15
Publisher: The Institute of Electronics, Information and Communication Engineers
7AM2-C-5 Construction of artificial ameba changing its morphology by biological molecular motor

Hiratsuka Yuichi, Nitta Takahiro, Nomura Shin-ichiro M.

2013　(5)　247-248　2013/11/04
Publisher: The Japan Society of Mechanical Engineers

More details Close

In this study, we challenged to construct an ameba-type molecular robot, which was changed its morphology using activity of motor protein trigged by calcium signal. An artificial kinesin which we designed, was formed tetramer complex by calcium ion signal and transformed the microtubule networks to aster or stress fiber-like structures. We tried to synthesize a droplet or liposome containing this microtubule networks as artificial cytoskeleton.
細胞抽出液の濃縮技術-細胞再構築に向けた挑戦-

野村M. 慎一郎, 藤原慶

月刊バイオインダストリー　30　(8)　59-65　2013/08/12
Publisher: シーエムシー出版
ISSN： 0910-6545
Construction of an in vitro model of a living cellular system

K. Yoshikawa, S. M. Nomura, K. Tsumoto, K. Takiguchi

The Minimal Cell: The Biophysics of Cell Compartment and the Origin of Cell Functionality　173-193　2011
Publisher: Springer Netherlands
DOI： 10.1007/978-90-481-9944-0_10 　
Degassing-Assisted Patterning of Cell Culture Surfaces

Chunxiong Luo, Xiaofang Ni, Li Liu, Shin-ichiro M. Nomura, Yong Chen

BIOTECHNOLOGY AND BIOENGINEERING　105　(4)　854-859　2010/03

DOI： 10.1002/bit22586 　

ISSN： 0006-3592
GFP synthesis in giant liposomes using the in vitro translation system of Thermococcus kodaks

Kazuaki Yamaji, Tamotsu Kanai, Shin-Ichiro M. Nomura, Kazunari Akiyoshi, Makiko Negishi, Haruyuki Atomi, Kenichi Yoshikawa, Tadayuki Imanaka

20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science　176-181　2009

DOI： 10.1109/MHS.2009.5351893 　
細胞機能の実空間モデリング

野村 M, 慎一郎, 山田彩子, 吉川研一

生物物理　48　(3)　174-179　2008
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.48.174 　

ISSN： 0582-4052

More details Close

Cell-modeling research has been attracting increasing interests, as a potential methodology to understand the structure and function of living cells. We describe the recent development on the studies toward the construction of an artificial-cell-model. It is shown that protein synthesis of gene transcription/translation reaction proceeds within lipidic artificial-cell-models (in lipo), cell-sized liposomes, and water-in-oil droplets. As an experimental example, we describe the in lipo synthesis of functional membrane proteins. We also present the effects of confinement on the structural transition of actin filaments inside cell-sized compartments. We propose a theoretical model on the epigenetic control of the On/Off switching of genetic activity.<br>
24aPS-129 Localization of cell-free synthesized membrane protein in phase-separated giant liposome membrane.

Nomura M. Shin-ichiro, Akiyoshi Kazunari

Meeting abstracts of the Physical Society of Japan　62　(2)　395-395　2007/08/21
Publisher: The Physical Society of Japan (JPS)
ISSN： 1342-8349
Giant Phospholipid Vesicles Entrapping Giant DNA

Shin-Ichirou Nomura, Kenichi Yoshikawa

Giant Vesicles: Perspectives in Supramolecular Chemistry　6　313-317　2007/03/12
Publisher: wiley
DOI： 10.1002/9780470511534.ch23 　
A Communication Interface Using Vesicles Embedded with Channel Forming Proteins in Molecular Communication

Y. Moritani, S. Hiyama, S. M. Nomura, K. Akiyoshi, T. Suda

2007 2ND BIO-INSPIRED MODELS OF NETWORKS, INFORMATION AND COMPUTING SYSTEMS (BIONETICS)　140-+　2007

DOI： 10.1109/BIMNICS.2007.4610101 　
Cellularmembrane-like structure of tubular-linked liposome network

Shin Ichiro M Nomura, Shin Ichiro M Nomura, Kazunari Akiyoshi, Kazunari Akiyoshi

Polymer Preprints, Japan　55　5231-5232　2006/12/01
Function of in situ synthesized membrane protein in the presence of cell-size liposome

Wakiko Asayama, Wakiko Asayama, Satoshi Kondoh, Shin Ichiro M Nomura, Shin Ichiro M Nomura, Kazunari Akiyoshi, Kazunari Akiyoshi

Polymer Preprints, Japan　55　2016　2006/10/19
Constructing functional cell model: cell-sized liposome engineering

Annual reports of the Institute of Biomaterials and Bioengineering　40　27-31　2006
Publisher: 東京医科歯科大学生体材料工学研究所
ISSN： 1345-2886
Nanogel engineered designs for polymeric drug delivery

Nobuyuki Morimoto, Shin-ichiro M. Nomura, Naomi Miyazawa, Kazunari Akiyoshi

POLYMERIC DRUG DELIVERY II: POLYMERIC MATRICES AND DRUG PARTICLE ENGINEERING　924　88-101　2006

ISSN： 0097-6156

eISSN： 1947-5918
A molecular communication interface using Liposomes with gap junction proteins Peer-reviewed

Yuki Moritani, Shin-ichiro M. Nomura, Satoshi Hiyama, Kazunari Akiyoshi, Tatsuya Suda

2006 1ST BIO-INSPIRED MODELS OF NETWORK, INFORMATION AND COMPUTING SYSTEMS　154-154　2006

DOI： 10.1109/BIMNICS.2006.361816 　
Self-organaized tubular-linked liposome network

Shin-ichiro M. Nomura, Kazunari Akiyoshi

2006 IEEE INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE　13-+　2006

DOI： 10.1109/MHS.2006.320309 　

ISSN： 2474-378X

eISSN： 2474-3798
細胞機能をどこまで組み立てられるか? セルサイズリポソーム工学から人工細胞モデルヘ

野村 M, 慎一郎, 秋吉一成

化学と工業　2006
Characteristics of nanogel-DNA complexes

Shin Ichi Sawada, Naomi Miyazawa, Shin Ichiro M Nomura, Kazunari Akiyoshi

Polymer Preprints, Japan　54　2148　2005/12/01
Morphological dynamics and transcription/translation reactions in cell-sized lipid vesicles

T Hamada, Y Sato, SM Nomura, K Tsumoto, T Nagasaki, K Yoshikawa

BIOPHYSICAL JOURNAL　88　(1)　238A-238A　2005/01

ISSN： 0006-3495
Giant vesicle as a simple model of a living cell: Construction of biochemical microreactors;

Kanta Tsumoto, Shin-ichiro M. Nomura, Tsutomu Hamada, Koji Kubo, Tetsuro Yoshimura, Kenichi Yoshikawa

IEEE Proceedings of the International Symposium on Micro-NanoMechatronics and Human Science, MHS 2005 Micro-Nano COE　85-89　2005
14aPS-88 Lipid Nanotubular Network Connection : giant liposome organization

Nomura Shinichiro, Akiyoshi K., Motoike Ikuko

Meeting abstracts of the Physical Society of Japan　59　(2)　259-259　2004/08/25
Publisher: The Physical Society of Japan (JPS)
ISSN： 1342-8349
勝手に動く超分子システムをつくる-さらなる生物らしさをめざして-

野村 M. 慎一郎

生物物理　43　(3)　142-145　2003
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.43.142 　

ISSN： 0582-4052
神経細胞の「形」とガングリオシド

野村 M. 慎一郎, 板谷綾子, 小野直亮, 吉川研一, 秋吉一成

形の科学会誌 = Bulletin of the Society for Science on Form　17　(1)　33-34　2002/06/01

ISSN： 0915-6089
Transportation of a single giant DNA with optical tweezers (2)

Ichikawa M. S., Nomura M., Kubo K.

Meeting abstracts of the Physical Society of Japan　56　(1)　311-311　2001/03/09
Publisher: The Physical Society of Japan (JPS)
ISSN： 1342-8349
Transportation of a single giant DNA with optical tweezers (1)

Nomura M. S., Ichikawa M.

Meeting abstracts of the Physical Society of Japan　56　(1)　310-310　2001/03/09
Publisher: The Physical Society of Japan (JPS)
ISSN： 1342-8349
Periodic Bursting of Dispersed Beads in μm-Sized Laser-Field

77　(2)　334-335　2001
Publisher: 物性研究刊行会
ISSN： 0525-2997
Towards proto-cells: "Primitive" lipid vesicles encapsulating giant DNA and its histone complex

Shin-Ichirou M. Nomura, Yuko Yoshikawa, Kenichi Yoshikawa, Olivier Dannenmuller, Sylvette Chasserot-Golaz, Guy Ourisson, Yoichi Nakatani

ChemBioChem　2　(6)　457-459　2001
Publisher: Wiley-VCH Verlag
DOI： 10.1002/1439-7633(20010601)2:6<457::AID-CBIC457>3.0.CO;2-F 　

ISSN： 1439-4227
Dynamics in Morphorogical Change of Giant Liposome Formed with Natural Swelling

NOMURA S., MAGOME N., YOSHIKAWA K.

Biophysics　37　S196　1997/10
Publisher: The Biophysical Society of Japan General Incorporated Association
ISSN： 0582-4052

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Books and Other Publications 12

Systemization Technology for Molecular Robots. In: Murata, S. (eds) Molecular Robotics

Nomura, Si, M

Springer, Singapore　2022/08

ISBN: 9789811939877
Biomolecules for molecular robot structures, in Encyclopedia of Robotics.

Nomura, S.-i. M, Archer R. J

2022
Mechanism and Machine Science: Select Proceedings of Asian MMS 2018 (Lecture Notes in Mechanical Engineering)

Shin-ichiro M. Nomura

2020/07/02

ISBN: 981154476X
"Micron-sized Molecular Robots" in Stimuli-Responsive Polymers Handbook

NOMURA M. Shin-ichiro

NTS　2018/12
人工細胞モデルとコミュニケーション能，細胞を創る・生命システムを創る, 実験医学増刊

野村 M, 慎一郎, 上田泰己

0　2011
Construction of an In Vitro Model of a Living Cellular System, in The Minimal Cell

K. Yoshikawa, S.M. Nomura, K. Tsumoto, K. Takiguchi, Edited by, P.L. Luisi

0　2011
細胞計算によるDrug Delivery System，自己組織化ハンドブック第3編システム・デバイス編第4節システム

野村 M. 慎一郎

0　2009
ナノチューブ-リポソームネットワーク，有機・無機・金属ナノチューブ—非カーボンナノチューブ系の最新技術と応用展開

野村慎一郎, 秋吉一成

0　2008
タンパク質合成能を有する人工細胞とネットワーク化，リポソーム応用の新展開～人工細胞の開発に向けて～

野村 M, 慎一郎, 秋吉一成

0　2005
ドラッグデリバリシステム，「図解バイオ活用技術のすべて」

野村 M, 慎一郎, 秋吉一成

0　2004
人工細胞システムをつくる，「ナノテクノロジーハンドブック」

野村慎一郎, 秋吉一成

0　2003
Toward the Creation of an Artificial Cellular System，Precision Polymers and Nano-Organized Systems

K. Yoshikawa, S.-i. Nomura(Edited, by T. Kunitake, S. Nakahama, S. Takahashi, N. Toshim

0　2000

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Presentations 20

An amoeba-type cell-sized molecular robot and the beyond International-presentation Invited

The 79th Okazaki conference, Okazaki　2018/08/31
Development of an amoeba-type molecular robot International-presentation Invited

ICNN5　2018/08/22
Amoeba-type cell-sized molecular robot International-presentation Invited

2018 International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS2018)　2018/07/06
Molecular Robotics: yet another artificial cell International-presentation Invited

The Active Matter Workshop 2018　2018/01/19
Molecular Robot with Switching Molecular Motors International-presentation Invited

International Symposium on Nanomedicine 2017(ISNM2017)　2017/12/13
Non Natural Tiny-wet Machine: Molecular Robot International-presentation Invited

2017 International Symposium on Micro-NanoMechatronics and Human Science (MHS2017)　2017/12/03
アメーバ型分子ロボットの構築 Invited

野村慎一郎

バイオ高分子研究会　2017/09/22
物質転送ベシクル:細胞内への大規模物体導入法 Invited

野村慎一郎

ミトコンドリアサイエンスワークショップ2017　2017/07/10
物質転送ベシクル：細胞内への大規模物体導入法 Invited

野村慎一郎

第69回日本細胞生物学会大会　2017/06/13
Molecular Robot: An artificial molecular system constructed by integration of molecular machines International-presentation Invited

ICRA2017 workshop　2017/06/02
Molecular robots under construction: amoeba-type artificial cell model International-presentation Invited

野村慎一郎, 佐藤佑介, 平塚祐一, 川岸由, 小椋利彦, 川又生吹, 村田智

第64回応用物理学会春期学術講演会　2017/05/15
Making a cell model: membrane protein expression/functioning on giant liposome International-presentation

Synthetic biology: from the present into the future　2010/03
膜タンパク質の直接無細胞合成による細胞モデルの多機能化

「細胞を創る」研究会2.0　2009/10
無細胞膜タンパク質合成からはじめる人工細胞モデル

「細胞を創る」研究会1.0　2008/10
Artificial cell models of membrane protein synthesizing liposome International-presentation

International Workshop on Physical Phenomena in Multi-Component Membranes　2008/03
人工細胞的手法による膜タンパク質機能化リポソームの調製と評価

分子情報通信(Molecular Communication)のサイエンス基盤　2007/09
機能的細胞モデルの構成

人工知能学会ナチュラルコンピューティング研究会　2007/07
GapJunction機能を有するリポソームDDSの開発

再生医療・細胞治療研究会　2005/10
Making giant liposome: towards artificial cellular system I International-presentation

The first general meeting of the Korean Society for Synthetic Biology (KSSB2005)　2005/02
熱的非平衡条件下における単一高分子鎖の折りたたみ／解きほぐし周期振動

第10回非線形反応と共同現象研究会　2000/11

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Industrial Property Rights 11

細胞内へのミトコンドリア導入方法、外因性ミトコンドリアが導入された細胞及び動物の製造方法

小椋利彦, 野村慎一郎, 齋藤明

Property Type: Patent
細胞内へ物体を導入する方法

小椋利彦, 野村慎一郎, 齋藤明

Property Type: Patent
リポソームの製造方法

野村慎一郎

Property Type: Patent
リポソームの製造方法

野村慎一郎

特許第5669058号

Property Type: Patent
リポソーム及びこれを用いた細胞に対する物質注入方法

森田育男, 秋吉一成, 野村慎一郎

Property Type: Patent
リポソームを用いたタンパク質の製造方法

秋吉一成, 野村慎一郎, 朝田晃一

Property Type: Patent
DNA転写反応の制御方法

吉川研一, 湊元幹太, 秋田谷龍男, 山田彩子, 久保康児, 野村慎一郎, 牧田直子, ルッケル,フランソワ・バレンティン

Property Type: Patent
レーザー駆動微小モーター

眞山博幸, 野村慎一郎, 吉川研一, 新井史人

Property Type: Patent
レーザー駆動微小モーター

眞山博幸, 野村慎一郎, 吉川研一, 新井史人

特許第4175592号

Property Type: Patent
リポソーム及びその製造方法

吉川研一, 野村慎一郎

Property Type: Patent
リポソーム及びこれを用いた細胞に対する物質注入方法

森田育男, 秋吉一成, 野村慎一郎

特許第5057362号

Property Type: Patent

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Research Projects 25

Construction of Artificial Liquid Intelligence Based on Molecular-Digital Interactions

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (S)

Institution: Tokyo Institute of Technology

2024/04/01 - 2029/03/31
Philosohpical development of the behavioral inhibition network hypothesis of the mind

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Shinshu University

2023/06/30 - 2026/03/31
Management / Public Relations / International Activity Support of Molecular Cybernetics

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Transformative Research Areas (A)

Category: Grant-in-Aid for Transformative Research Areas (A)

Institution: Tohoku University

2020/11/19 - 2025/03/31
Development of Molecular Information Transmitting Devices for Minimal Artificial Brain

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Transformative Research Areas (A)

Category: Grant-in-Aid for Transformative Research Areas (A)

Institution: Tohoku University

2020/11/19 - 2025/03/31
AutoMatter: Toward creation and expansion of programmable micro-active matter

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S)

Category: Grant-in-Aid for Scientific Research (S)

Institution: Tohoku University

2020/08/31 - 2025/03/31
Construction and control of macrophage-like molecular robots by digital-to-molecular information transformation

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tokyo Institute of Technology

2020/04/01 - 2024/03/31
AMED-CREST「細胞質から核に至る力覚機構の解明と新技術開発から医学展開を目指す基礎研究」 Competitive

System: The Other Research Programs

2015/12 - 2021/03
Development of "artificial muscle' ink for 3D print of microrobots

Hiratsuka Yuichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Japan Advanced Institute of Science and Technology

2017/06/30 - 2019/03/31

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In recent years, the rapid development of three-dimensional printing (3-D printing) opens new technologies. On the other hands, the construction of actuator by 3-D printer is not realized, so that actuator parts will have to be assembled into the frame part fabricated by 3-D printer after printing. Recently, we succeeded in constructing an artificial muscle using motor proteins by UV light illumination similar to a 3-D photo printing. We also succeeded in the operation of millimeter scaled micro-robot using this artificial muscle. In this project, we developed ‘artificial muscle ink’ containing the motor proteins, and began to develop new techniques for the 3-D printing of actuator of micro-robot.
Multicellular analog/digital computing with cellular neighbouhood communication validated by Synthetic Biology

Yamamura Masayuki, SUZUKI Yasuhiro, NOMURA Shin-ichiro, ITO Hiroshi, SEKINE Ryoji

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tokyo Institute of Technology

2015/04/01 - 2018/03/31

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Synthetic Biology meets the stage to treat collective activity of mixed species cells. However, conventional researches stay in the level of controlling homogeneous spatial structure by using small chemicals. We proposed a computational model which can be validated by biological experiments with Synthetic Biology. We selected a layered mat structure of microorganisms in natural hot springs as a testbed. We performed a meta-genome analysis on microbe mats under artificially controlled environment by LED lights. The results show the species composition will be changed according to the strength of the light and suggest a method to control the function of collective activities of mixed species cells.
Developing DNA / RNA molecular devices for constructing a new generation of artificial cells. Competitive

NOMURA Shin-ichiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2015/04/01 - 2018/03/31

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In this project we developed functional molecular devices using DNA / RNA. It would lead to the realization of new generation artificial cells showing new functions in the future. We performed design and functional evaluation of artificial molecular device group and developed a method to actually introduce the device into the cell. As a result, we achieved the goal of constructing artificial RNP (complex of RNA and protein) nanostructures in mammalian cells, and controling the cell fate. In addition, artificial cells that switch between motive-state and stationary-state were constructed using DNA complex molecular system (molecular clutch) that controls on / off linkage with lipid membrane. In addition, all the results of the molecular devices we constructed were also published in the journal papers. The results of this project will be used as a basis for constructing a new generation of artificial cells.
Research and Development of Amoeba-type Molecular Robots

Konagaya Akihiko, TOYOTA Taro, HAYASHI Masahito

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Institution: Tokyo Institute of Technology

2012/06/28 - 2017/03/31

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The joint project between the amoeba robot team and other teams developed a giant liposome based amoeba-type molecular robot prototype whose shape can be changed by DNA signals by means of integrating various element technologies including molecular actuators and molecular control systems. As for the molecular actuators, the team developed (1) molecular crutch which control the connection between giant liposome and molecular motors, (2) photo-induced growth system of peptide nanofibers propelling giant liposomes with comet tail-like mechanism, (3) reversible morphological control system of tubulin-encapsulating giant liposomes by hydrostatic pressure, and (4) calcium concentration dependent morphological changing microtubule networks. As for the molecular control systems, the team developed (5)photo-sensitive DNA strands attached on microtubules, (6) microtubule motion pattern controlling system, and (7) particle-based real-time simulation system to reproduce the motion pattern.
感覚と知能を備えた分子ロボットの創成：アメーバ型分子ロボット実現のための要素技術開発とその統合 Competitive

System: Grant-in-Aid for Scientific Research

2012/09 - 2017/03
Towards constructing artificial cell model with cellular components

NOMURA Shin-ichiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2013/04/01 - 2015/03/31

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This project aims to construct a cell model by using giant unilamellar vesicle (GUV) with extracted live cell components. During the project, we developed the novel method for introducing micro objects (~1um) into live cells, by using electrofusion with GUVs. Briefly, dispersed cells and GUVs were placed into an electrofusion chamber, and were exposed to an AC field to align cells-GUVs, and were also exposed DC pulse to induce transient electrofusion. The processed cell reached confluence with entrapping specified sizes of fluorescent beads. The transfer efficiency was evaluated by using fluorescence microscopy and flow cytometry. Delivering magnetic beads, DNA origamis, plasmids were also implemented. Introduction of artificial objects into live cells is an important topic in biotechnology. We expect that our novel method will be used for elucidation of cell mechanisms and creation of artificial cell which consists of cellular components.
Development of molecular robotics based on DNA nanoengineering Competitive

MURATA Satoshi, KUZUYA Akinori, TAKINOUE Masahiro, SEKIYAMA Kosuke, NOMURA Shin-ichiro, FUJIMOTO Kenzo, TAKEUCHI Shyoji, KOBAYASHI Satoshi, KAWAMATA Ibuki, MORITA Masamune, HAMADA Shogo

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S)

Category: Grant-in-Aid for Scientific Research (S)

Institution: Tohoku University

2010/04/01 - 2015/03/31

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In this research project, aiming at developing fundamental techniques to construct molecular robots composed of molecular devices rigorously designed from scratch, which are able to adapt to various environmental changes. The developed technologies based on DNA nano-engineering include (1) Techniques to fabricate compartments which compose the body of molecular robot containing molecular devices, (2) Techniques to realize interface, which realizes molecular communication across the compartment, (3) Techniques to control the molecular computation in molecular robots and intra-and inter-molecular robot communication, (4) Techniques to design cooperative behaviors of swarm of multiple molecular robots.
自己複製能をもつRNA酵素を基盤とした人工生命体モデルの創出 Competitive

System: Grant-in-Aid for Scientific Research

2011/04 - 2013/03
高次構造制御による膜タンパク質機能発現リポソームの構築 Competitive

System: JST Basic Research Programs (Precursory Research for Embryonic Science and Technology :PRESTO)

2008/10 - 2012/03
Synthetic approaches to investigate artificial living systems by RNA self-replicating ribozymes

SAITO Hirohide, NOMURA Shin-ichiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Kyoto University

2011 - 2012

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One of the main goals in science is to solve the problem of origin of life: How evolvable living systems have emerged from chemical molecules such as nucleic acids? RNA can function both as an informational molecule and as a catalyst in living organisms. This duality is the premise of the RNA world hypothesis in the origin of life theory. Specially, it is believed that RNA ribozyme that facilitates self-replicating reaction is one of the most essential molecules in primitive life. To verify whether RNA has the potential for self-replication, we constructed a new RNA self-assembling ribozyme that could have conducted an evolvable RNA self-replication reaction. The artificially designed, in vitro selected ligase ribozyme was employed as a prototype for a self-assembling ribozyme. We propose that our system implies the significance of RNA tertiary motifs in the early RNA molecular evolution of the RNA world. Furthermore, we designed the synthetic RNA sequence for making higher-order membrane like structure. Giant membrane structure might be observed by using fluorescence microscopy. Their forming condition should be investigated and the result should be reported as a research article. These results may provide a possible RNA world in which both replicator and the compartment could be made by RNA. This concept might provide an alternative way to investigate the problem of the origin of life.
To develop neural chips using hPSC-derived neurons for facilitating in-vitro investigations of neural networks by nanofibers

CHEN Yong, LIU Li

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Kyoto University

2010 - 2012

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We construct well-designed neural networks by using either embryonic or induced pluripotent stem cells. Both topographic and biochemical cell growth cues are patterned to guide the growth and connation of derived axons and dendrites. In addition, aligned nanofibers-based topographic and microfluidic circuits as well as integrated microelectrode arrays are used for more active control and monitoring cellular network activities. If successful, the derived neuron chips can be useful as model system for advanced study of drug screening.
Physico-chemical approach on the construction of functional denovo cell

YOSHIKAWA Kenichi, IMANAKA Tadayuki, AKIYOSHI Kazunari, NOMURA Shinichiro, SETO Hidenori, KITAHATA Hiroyuki, ARUGA Takayuki, KANAI Tamotsu, INAGAKI Shio, SATO Yuko, ICHIKAWA Masatoshi, TAKINOUE Masahiro, IMAMURA Hisako

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Creative Scientific Research

Category: Grant-in-Aid for Creative Scientific Research

Institution: Kyoto University

2006 - 2010

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1) We have successfully constructed a model experimental system exhibiting robust on/off switching on genetic activity accompanied by the structural transition of a giant DNA molecule. 2) It is shown that micro-confinement causes specific phase-segregation. We demonstrated marked acceleration of gene-expression reaction in an artificial model cell. 3) We proposed a novel theoretical model to explain the emergent process of the morphology in multicellular systems.
機能性膜を自動生成する人工細胞モデルの構成と評価

野村慎一郎, 澤田晋一, 陳勇, 劉莉

Offer Organization: 日本学術振興会

System: 科学研究費助成事業特定領域研究

Category: 特定領域研究

Institution: 京都大学

2008 - 2009

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本研究は,機能性膜を自動生成する人工細胞モデルの構成と評価を目的に掲げた.具体的には,細胞サイズのリポソームを用い,その場で機能的な膜タンパク質を遺伝情報に従って無細胞的に合成・提示する系の構築手法と評価系の確立を行った.本研究では特に,膜タンパク質の1)発現(無細胞合成),2)局在(膜への挿入),3)配向,4)活性(機能)と,5)構成されたプロテオリポソームの特性の評価を通じて,機能性膜を自動生成する人工細胞モデルの標準的な調製法の提示を目指した.細胞間の物質輸送を司る4回膜貫通型タンパク質コネキシンについて,リポソーム環境における無細胞発現を行い,直接構成と機能化の測定を行った.その結果,リポソームから培養細胞への物質輸送が確認された(Biomaterials, 2009).さらにリポソームを構成する脂質組成による発現および挿入効率の違いを求め,サイズおよび膜に対する発現場所の違いによる配向性を示した.現在論文投稿中である.評価に関しては,細胞サイズリポソームを流路中で安定に捕捉し,直接観察を可能にするデバイスを構築した.これを利用した細胞サイズリポソームの抗体染色手法を開発し学会発表を行った.現在,論文投稿中である.これらの知見は,多種多様な生命現象は生体分子の集合体からいかにして構成されうるのか,という問いに答えるため,実験的に人工細胞モデル構築する研究において重要な進展であると考えられる.(加えて,研究グループとして新生タンパク質の安定保持を可能とする新奇材料である疎水化多糖ナノゲルを用いた薬剤輸送システムを開発し,査読つき論文として報告を行った.さらにMEMS技術を用いた培養細胞の局所制御システムの構築についても報告している.)
Drug delivery by liposome membrane phase separation mechanism

NOMURA Shinichiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

Category: Grant-in-Aid for Young Scientists (A)

Institution: Kyoto University

2007 - 2008
化学物質の伝達に基づき自律駆動する人工細胞ネットワークの構築

野村慎一郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業特定領域研究

Category: 特定領域研究

Institution: 東京医科歯科大学

2006 - 2007

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本研究は,細胞と部分的に接合して得たエネルギーを駆動力とする人工細胞ネットワークの構成を目的としたものである.具体的には,1)細胞との間に物質交換チャネルを形成する膜タンパク質を提示させるリポソームを構築し,2)細胞接着因子を導入してゲル内でリポソームネットワークを固定化した構造を構築する.3)1)と2)を組み合わせることにより,リポソームネットワークが培養細胞群の表面に接着して個々にチャネルを形成し,化学物質の交換を実現する研究を順次おこなってきた.1)について,基礎的な成果を論文(J. Biotechnol. 2008)および国際会議論文(ICBP2007, MHS2007)にて発表し,さらに細胞との物質交換チャネルを形成する膜タンパク質のリポソームへの導入について論文投稿中である.2)について,細胞接着因子(ペプチド融合脂質)を導入したリポソームネットワークをゲル中にて構築し,培養細胞との相互作用確認を行った.接着因子の有無およびネットワーク形成の有無によって,ゲル表面への細胞接着能の制御が確認された(論文作成中).また,3)について,リポソームと細胞との間での親水性蛍光色素分子の交換を確認(ICBP2007)している.これらの知見より,細胞からリポソームネットワーク側に流入したATP等の小分子を利用してリポソーム内に封入された反応系の持続的な進行が実現できると考えられる.自律的に駆動する人工細胞ネットワークは,培養細胞の化学情報の直接処理および利用を可能とする新規ツールとして働くと期待出来る.
脂質ナノチューブネットワークを用いた新規DDSの設計

野村慎一郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業若手研究(B)

Category: 若手研究(B)

Institution: 東京医科歯科大学

2005 - 2006

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本研究では,固定化された脂質ナノチューブネットワーク構造を新規DDSとして用いるための基礎データを収集すること目的としている.これまでに,親水性ゲル中に固定化された脂質ナノチューブネットワークの安定性ならびに外部刺激(温度,pH,コレステロールを一定割合で含むチューブネットワークに対するゲル外部からのシクロデキストリン添加等)による断片化による構造安定性の変化の調査を行った.これらの挙動にはゲルの強度と脂質膜の状態が大きく影響することが予想されたため,各サンプルの脂質膜および内水相の状態を確認するために随時,蛍光顕微鏡による直接観察を行った結果,ゲル強度および脂質濃度の初期条件に明確に依存したネットワーク形成能の違いが確認された.さらに,蛍光分光光度計を用いて,微量の蛍光脂質(脂溶性薬物モデル)により予めラベルした脂質チューブのゲル内へのネットワークの保持効率と,外部刺激による構造の崩壊〜外部溶液中への放出挙動を測定した.このネットワークを形成したチューブ内部には巨大分子(DNA,166kbp)をも封入可能であることが直接観察によって示された.また,封入されたDNA分子が,単一分子レベルでブラウン運動をおこなっている様子が高感度撮影により確認され,チューブ内部に親水性の空間が保持されていることが明らかになった.これらの結果を論文としてまとめたものをMHS2006にて発表した.
分子シャペロン機能を有するナノゲルキャリアによる新規DDSの開発

秋吉一成, 松田修, 岩崎泰彦, 渡辺昭彦, 森本展行, 野村慎一郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業特定領域研究

Category: 特定領域研究

Institution: 東京医科歯科大学

2005 - 2005

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本研究では、これまでの知見をもとに、独自に開発した分子シャペロン機能を有する自己組織化ナノゲル複合体を用いて、癌治療における新しいDDSの設計とその応用を目的とする。悪性腫瘍に対する治療の一つとして、各種の免疫療法が従来より試みられている。このような治療においてインターロイキン12(IL-12)は、高い抗腫瘍効果を持つサイトカインの一つとして注目されている。しかしながら、サイトカインを用いた免疫療法においては、その短い半減期に示される生体内での不安定性や、大量・反復投与による副作用などが、有効な抗腫瘍効果を得るための障壁となっているのも事実である。そこで我々は、IL-12の生体内投与時における安定性を向上する目的で、コレステロール基を修飾したプルラン(CHP)よりなるヒドロゲルナノ粒子(ナノゲル)(30nm)のキャリアとしての機能に着目した。マウスrecombinant IL-12とCHPとを種々の条件下で混合し、IL-12・ナノゲル複合体微粒子を調製した。その後ELISA法にてIL-12の活性を計測し、ナノゲルとの複合化条件を検討した。調製したIL-12・ナノゲル複合体微粒子の機能を評価するために、マウスの脾臓より採取したCD4陽性T細胞をin vitroで刺激し、産生されるIFN-γをELISAで経時的に測定した。さらにin vivoでの作用を以下の実験で検討した。Balb/cマウス由来の肉腫細胞、CSA1M細胞を同系マウスの皮下に接種し、皮下腫瘍モデルマウスを作成した。これにIL-12・ナノゲル複合体微粒子を投与し、腫瘍体積の変化を計測した。得られた結果より、IL-12はCHPナノゲルと相互作用して効率よく複合体微粒子を形成し、その後ナノゲルから生理活性を失うことなく放出できるシャペロン機能を発揮することが確認できた。またin vivo実験において、IL-12・ナノゲル複合体微粒子を6日間隔で3回という非常に少ない投与により、腫瘍の有意な増殖抑制が得られた。ナノゲル担体が優れた徐放機能を有していることがわかった。さらに、徐放性の優れた架橋ナノゲル(100-200nm)の調整法も確立した。本法は、サイトカイン免疫療法の欠点を克服するための新しいテクノロジーを切り開く可能性があると考えられる。
荷電高分子の相転移由来の非線形特性に基づき動作する分子機械モデルの研究

野村慎一郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業特別研究員奨励費

Category: 特別研究員奨励費

Institution: 東京医科歯科大学

2003 - 2005

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本研究は,荷電高分子鎖からなる分子機械(核酸や酵素など)の振る舞いを,環境との相互作用に基づく相転移挙動をキーワードに実験的に解き明かすことを目的としている.本研究期間においては,細胞サイズの脂質二分子膜胞(ジャイアントリポソーム:GL)におけるDNAを鋳型としたタンパク質(GFP)発現系の構築に成功した.その結果,GL内部におけるGFPの発現率がバルク溶液中よりも約10倍高いとの基礎的な知見を得た(CHEMBIOCHEM誌(2003)掲載).この機構に対するアプローチとして,反応の素過程を一つずつ詳細に確認してゆくという手法と,他のタンパク質発現系を用いた場合の結果から微小空間の効果を演繹的に議論する手法の二つが考えられるが,生体高分子機械の動作環境を知る研究においては後者のアプローチを行い,よりシンプルな酵素反応および高分子の相転移現象における微小体積の効果を測定することで環境との相互作用による分子機械の働きについての知見が得られると期待できる.関連して,自発的なリポソームの形成時に共存しているDNA分子が,リポソーム内部に濃縮されているという実験結果を得ている(Chem.Phys.Lett.誌(2003)掲載).(平行して,外部溶液よりDNA分子を細胞内へとレーザーを用いて非接触に導入する手法を開発し,植物細胞のプロトプラストにDNA分子を選択的に導入することに成功した(Appl.Phys.Lett誌(2003)掲載)).これらの実験結果は,細胞サイズの閉環境をつくる脂質膜構造が,内包したDNAやタンパク質等の高分子・低分子やイオンとの相互作用により(それらと同じ濃度に調製された)バルク溶液中とは大きく異なる環境をあたえうることを示すものである.これによって生物が荷電高分子機械を用いる場の特性と意義を知るための直接的な研究基盤が形成されたと考えられる.

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Teaching Experience 7

デザインとエンジニアリング　東北大学工学研究院
分子ロボティクス基礎　東北大学大学院
システム工学　東北大学
数学物理学演習I　東北大学
ロボティクス特論
機械力学II
熱力学II

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Social Activities 2

東工大主催「ナノバイオコース」講師

2013/01/22 -

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先端環境材料・デバイス創製スクールナノバイオコースにて特別講義「リポソームと人工細胞」を行った。
高大連携地域公開講座（仙南高校サマーカレッジ）

2011/07/19 -

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細胞はなぜ作れないのか？〜工学部からみた生命〜

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翻訳：数理生理学・日本評論社，J.Keener, J. Sneyd著，中垣俊之監訳