Details of the Researcher

PHOTO

Sanshiro Kanazawa
Section
Graduate School of Dentistry
Job title
Senior Assistant Professor
e-Rad No.
60823466

Committee Memberships 1

  • The Japanese Society for Regenerative Medicine　a member of delegation

    - Present

Professional Memberships 5

  • 日本口腔科学会

    2024/07 - Present

  • 日本骨免疫学会

    2021/06 - Present

  • International Cartilage Repair Society

    2010/09 - Present

  • 日本再生医療学会

    2008/10 - Present

  • 日本口腔外科学会

    2005/04 - Present

Research Interests 3

  • cleft lip with palate

  • development of tooth

  • bone marrow

Research Areas 1

  • Life sciences / Surgical dentistry /

Awards 2

  1. award of excellence

    2023/06　Japanese Society of Osteoimmunology

  2. award of excellence

    2021/06　Japanese Society of Osteoimmunology

Papers 21

  1. Dental pulp cell–derived conditioned culture media promotes periodontal repair through immunomodulation and bone regeneration Peer-reviewed

    Sanshiro Kanazawa, Sayaka Ono, Hiroki Shinchi, Hiroka Kanno, Koji Ueda, Naozumi Ishimaru, Kunio Takemoto, Kazuto Hoshi

    Regenerative Therapy　33　101144-101144　2026/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.reth.2026.101144 　

    ISSN： 2352-3204

  2. Prevention of oral mucositis in patients undergoing cancer chemotherapy using betamethasone mouthwash: A multicenter randomized controlled trial protocol Peer-reviewed

    Sakiko Soutome, Tomofumi Hamada, Hiromi Nishi, Eiji Iwata, Hirokazu Nakahara, Toshiro Yamamoto, Tatsushi Matsumura, Masaya Nishikawa, Kei Tomihara, Koichiro Ueki, Mamoru Noda, Makoto Shimanishi, Madoka Funahara, Masami Kawahara, Yoshiko Yamamura, Shinpei Matsuda, Kensuke Yoshida, Sanshiro Kanazawa, Minako Otsuji, Nagato Natsume, Masahiro Umeda

    PLOS One　21　(4)　e0345991-e0345991　2026/04/08

    Publisher: Public Library of Science (PLoS)

    DOI： 10.1371/journal.pone.0345991 　

    eISSN： 1932-6203

    More details Close

    Background Oral mucositis is a frequent adverse effect of chemotherapy that significantly affects patients’ quality of life and treatment continuity. Despite its clinical relevance, effective preventive strategies remain limited. This study aims to compare the efficacy and safety of betamethasone mouthwash with those of sodium gualenate hydrate mouthwash for preventing chemotherapy-induced oral mucositis. Methods This Phase II, multicenter, open-label, randomized controlled trial aims to enroll 296 adult patients (≥18 years) undergoing chemotherapy for solid tumors (excluding head and neck and hematological cancers). Participants will be randomized 1:1 into two arms: a betamethasone mouthwash (intervention) group and a sodium gualenate hydrate mouthwash (control) group. The primary outcome is the prevention of Grade 1 oral mucositis, assessed using the Common Terminology Criteria for Adverse Events v3.0 and v5.0. Secondary outcomes include prevention of Grade 2–3 oral mucositis, the incidence of oral candidiasis, and chemotherapy completion rates. Data will be analyzed using Kaplan–Meier survival curves, log-rank tests, and Cox regression models. Expected Outcomes This study aims to establish betamethasone mouthwash as an effective preventive strategy for potentially enhancing the quality of life of patients with cancer. Trial registration This study was registered with the Japan Registry of Clinical Trials on September 20, 2024 (jRCTs071240060).

  3. Hepatocyte growth factor in biosheets promotes autonomous regeneration of cutaneous tissue after transplantation onto a full-thickness skin defect

    Keisuke Suzuki, Hiroko Komura, Ryo Konno, Yusuke Kawashima, Eiichiro Watanabe, Hiroki Sato, Kunio Matsumoto, Ryoko Inaki, Sanshiro Kanazawa, Yukiyo Asawa, Atsuhiko Hikita, Kazuto Hoshi, Jun Fujishiro, Makoto Komura

    Materials Today Bio　37　102969-102969　2026/04

    Publisher: Elsevier BV

    DOI： 10.1016/j.mtbio.2026.102969 　

    ISSN： 2590-0064

  4. intra-single cell sequencing (iSCseq) spotlights transcriptomic and epigenetic heterogeneity inside multinucleated osteoclast

    Hiroyuki Okada, Yuta Terui, Yasunori Omata, Masahide Seki, Shoichiro Tani, Junya Miyahara, Kenta Makabe, Asuka Terashima, Sanshiro Kanazawa, Masahiro Hosonuma, Fumiko Yano, Hiroshi Kajiya, Taku Saito, Yutaka Suzuki, Koji Okabe, Roland Baron, Sakae Tanaka, Ung-il Chung, Hironori Hojo

    2022/09/06

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2022.09.05.506360 　

    More details Close

    Abstract Multinucleated giant cells like osteoclasts (OCs) often contain >10 nuclei. This raises the question of whether nuclei residing in the same cytoplasm are all regulated similarly or whether they are heterogeneous, expressing a different set of genes regulated by nucleus-specific epigenetic mechanisms. We combined imaging of living cells with confocal microscope, picking of intracellular components inside a single cell using the Single Cellome™ System SS2000 (Yokogawa, Japan), allowing next generation sequencing at high resolution for mRNA (iscRNA-seq) and for DNA methylation (iscWGBS; whole genome bisulfite sequencing) of any cellular components including a single OC nucleus. Our results reveal that individual nuclei within the same cell are heterogeneous in terms of gene expression and epigenetic regulation, possibly affecting regional cell function.

  5. Hematopoietic-Mesenchymal Signals Regulate the Properties of Mesenchymal Stem Cells. International-journal

    Sanshiro Kanazawa, Hiroyuki Okada, Dan Riu, Yo Mabuchi, Chihiro Akazawa, Junichi Iwata, Kazuto Hoshi, Atsuhiko Hikita

    International journal of molecular sciences　23　(15)　2022/07/26

    DOI： 10.3390/ijms23158238 　

    eISSN： 1422-0067

  6. 生体内組織形成術iBTAの発展とbiosheet/biotubeによる臓器再生の小児外科疾患への応用の未来

    鈴木 啓介, 藤代 準, 吉田 真理子, 高澤 慎也, 古村 浩子, 浅輪 幸世, 金澤 三四朗, 中山 泰秀, 古村 眞

    日本外科学会定期学術集会抄録集　122回　DP-4　2022/04

    Publisher: (一社)日本外科学会

  7. Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties. International-journal

    Sanshiro Kanazawa, Hiroyuki Okada, Hironori Hojo, Shinsuke Ohba, Junichi Iwata, Makoto Komura, Atsuhiko Hikita, Kazuto Hoshi

    Scientific reports　11　(1)　15811-15811　2021/08/04

    DOI： 10.1038/s41598-021-94186-5 　

    ISSN： 2045-2322

  8. β-TCP/RCPハイブリッド足場に搭載する骨髄細胞の骨芽細胞分化培養期間の最適化

    梅山 遼, 山脇 孝徳, 金澤 三四朗, 高戸 毅, 疋田 温彦, 星 和人

    日本口腔科学会雑誌　70　(2)　127-127　2021/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  9. Optimization of culture duration of bone marrow cells before transplantation with a β-tricalcium phosphate/recombinant collagen peptide hybrid scaffold. International-journal

    Ryo Umeyama, Takanori Yamawaki, Dan Liu, Sanshiro Kanazawa, Tsuyoshi Takato, Kazuto Hoshi, Atsuhiko Hikita

    Regenerative therapy　14　284-295　2020/06

    DOI： 10.1016/j.reth.2020.04.005 　

    ISSN： 2352-3204

  10. Glial Fibrillary Acidic Protein as Biomarker Indicates Purity and Property of Auricular Chondrocytes. International-journal

    Satoru Nishizawa, Sanshiro Kanazawa, Yuko Fujihara, Yukiyo Asawa, Satoru Nagata, Motohiro Harai, Atsuhiko Hikita, Tsuyoshi Takato, Kazuto Hoshi

    BioResearch open access　9　(1)　51-63　2020

    DOI： 10.1089/biores.2019.0058 　

    ISSN： 2164-7860

  11. Three-dimensional changes of noses after transplantation of implant-type tissue-engineered cartilage for secondary correction of cleft lip–nose patients International-journal

    Kazuto Hoshi, Yuko Fujihara, Hideto Saijo, Kumiko Kurabayashi, Hideyuki Suenaga, Yukiyo Asawa, Satoru Nishizawa, Sanshiro Kanazawa, Sakura Uto, Ryoko Inaki, Mariko Matsuyama, Tomoaki Sakamoto, Makoto Watanabe, Madoka Sugiyama, Kazumichi Yonenaga, Atsuhiko Hikita, Tsuyoshi Takato

    Regenerative Therapy　7　72-79　2017/12

    DOI： 10.1016/j.reth.2017.09.001 　

    ISSN： 2352-3204

  12. Biological roles of glial fibrillary acidic protein as a biomarker in cartilage regenerative medicine. International-journal

    Sanshiro Kanazawa, Satoru Nishizawa, Tsuyoshi Takato, Kazuto Hoshi

    Journal of cellular physiology　232　(11)　3182-3193　2017/11

    DOI： 10.1002/jcp.25771 　

    ISSN： 0021-9541

    eISSN： 1097-4652

  13. Implant-type Tissue-engineered Cartilage for Secondary Correction of Cleft Lip-nose Patients: An Exploratory First-in-human Trial

    Kazuto Hoshi, Yuko Fujihara, Hideto Saijo, Yukiyo Asawa, Satoru Nishizawa, Sanshiro Kanazawa, Sakura Uto, Ryoko Inaki, Mariko Matsuyama, Tomoaki Sakamoto, Makoto Watanabe, Madoka Sugiyama, Kazumichi Yonenaga, Atsuhiko Hikita, Tsuyoshi Takato

    Journal of Clinical Trials　07　(03)　2017

    Publisher: OMICS Publishing Group

    DOI： 10.4172/2167-0870.1000315 　

    eISSN： 2167-0870

  14. Production of three-dimensional tissue-engineered cartilage through mutual fusion of chondrocyte pellets Peer-reviewed

    K. Hoshi, Y. Fujihara, Y. Mori, Y. Asawa, S. Kanazawa, S. Nishizawa, M. Misawa, T. Numano, H. Inoue, T. Sakamoto, M. Watanabe, M. Komura, T. Takato

    International Journal of Oral and Maxillofacial Surgery　45　(9)　1177-1185　2016/09/01

    Publisher: Churchill Livingstone

    DOI： 10.1016/j.ijom.2016.03.002 　

    ISSN： 1399-0020 0901-5027

  15. Preclinical and clinical research on bone and cartilage regenerative medicine in oral and maxillofacial region

    Tsuyoshi Takato, Yoshiyuki Mori, Yuko Fujihara, Yukiyo Asawa, Satoru Nishizawa, Sanshiro Kanazawa, Toru Ogasawara, Hideto Saijo, Takahiro Abe, Masanobu Abe, Hideyuki Suenaga, Yuki Kanno, Madoka Sugiyama, Kazuto Hoshi

    ORAL SCIENCE INTERNATIONAL　11　(2)　45-51　2014/05

    DOI： 10.1016/S1348-8643(14)00008-1 　

    ISSN： 1348-8643

    eISSN： 1881-4204

  16. Regenerative Cartilage made by Fusion of Cartilage Elements derived from Chondrocyte Sheets prepared in Temperature-Responsive Culture Dishes

    Yoshiyuki Mori, Sanshiro Kanazawa, Yukiyo Asawa, Tomoaki Sakamoto, Ryoko Inaki, Kazumi Okubo, Satoru Nagata, Makoto Komura, Tsuyoshi Takato, Kazuto Hoshi

    Journal of Hard Tissue Biology　23　(1)　101-110　2014

    DOI： 10.2485/jhtb.23.101 　

    ISSN： 1341-7649

    eISSN： 1880-828X

  17. Usefulness of Agarose Mold as a Storage Container for Three-Dimensional Tissue-Engineered Cartilage Peer-reviewed

    Yoshiyuki Mori, Sanshiro Kanazawa, Makoto Watanabe, Hideyuki Suenaga, Kazumi Okubo, Satoru Nagata, Yuko Fujihara, Tsuyoshi Takato, Kazuto Hoshi

    Materials Sciences and Applications　04　(08)　73-78　2013

    Publisher: Scientific Research Publishing, Inc.

    DOI： 10.4236/msa.2013.48a010 　

    ISSN： 2153-117X

    eISSN： 2153-1188

  18. Recent trends in cartilage regenerative medicine and its application to oral and maxillofacial surgery

    Kazuto Hoshi, Yuko Fujihara, Yukiyo Asawa, Satoru Nishizawa, Sanshiro Kanazawa, Tomoaki Sakamoto, Makoto Watanabe, Toru Ogasawara, Hideto Saijo, Yoshiyuki Mori, Tsuyoshi Takato

    ORAL SCIENCE INTERNATIONAL　10　(1)　15-19　2013/01

    DOI： 10.1016/S1348-8643(12)00049-3 　

    ISSN： 1348-8643

    eISSN： 1881-4204

  19. Tissue responses against tissue-engineered cartilage consisting of chondrocytes encapsulated within non-absorbable hydrogel. International-journal

    Sanshiro Kanazawa, Yuko Fujihara, Tomoaki Sakamoto, Yukiyo Asawa, Makoto Komura, Satoru Nagata, Tsuyoshi Takato, Kazuto Hoshi

    Journal of tissue engineering and regenerative medicine　7　(1)　1-9　2013/01

    DOI： 10.1002/term.458 　

    ISSN： 1932-6254

  20. Application of floating cells for improved harvest in human chondrocyte culture

    Kazumichi Yonenaga, Satoru Nishizawa, Yuko Fujihara, Yukiyo Asawa, Sanshiro Kanazawa, Satoru Nagata, Tsuyoshi Takato, Kazuto Hoshi

    Biomedical Research　33　(5)　281-289　2012

    DOI： 10.2220/biomedres.33.281 　

    ISSN： 0388-6107

    eISSN： 1880-313X

  21. The optimal conditions of chondrocyte isolation and its seeding in the preparation for cartilage tissue engineering. International-journal

    Kazumichi Yonenaga, Satoru Nishizawa, Yuko Fujihara, Yukiyo Asawa, Kanazawa Sanshiro, Satoru Nagata, Tsuyoshi Takato, Kazuto Hoshi

    Tissue engineering. Part C, Methods　16　(6)　1461-9　2010/12

    DOI： 10.1089/ten.TEC.2009.0597 　

    ISSN： 1937-3384

    eISSN： 1937-3392

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Misc. 16

  1. Osteocyte Subsets through the Lens of Subcellular Spatial and Interspecies Single Cell Atlas-level Transcriptomics for Bone

    Hiroyuki Okada, Shoko Onodera, Ayako Suzuki, Yoshiaki Kitaura, Takehiro Matsubara, Akinori Kanai, Shigeto Seno, Tomoaki Kurashige, Yohei Oshiro, Yasunori Omata, Asuka Terashima, Sanshiro Kanazawa, Fumiko Yano, Taku Saito, Masahide Seki, Francesca Gori, Junichi Kikuta, Toshifumi Azuma, Yutaka Suzuki, Sakae Tanaka, Roland Baron, Ung-il Chung, Hironori Hojo

    JOURNAL OF BONE AND MINERAL RESEARCH　39　376-376　2024/11

    ISSN： 0884-0431

    eISSN： 1523-4681

  2. 生きた細胞内シーケンスで迫る破骨細胞内ネットワーク

    岡田寛之, 岡田寛之, 岡田寛之, 寺島明日香, 谷彰一郎, 谷彰一郎, 金澤三四朗, 細沼雅弘, 小野寺晶子, 矢野文子, 小俣康徳, 小俣康徳, 鍛治屋浩, FRANSCESCA Gori, 斎藤琢, 岡部幸司, BARON Roland, 鄭雄一, 鄭雄一, 田中栄, 北條宏徳, 北條宏徳

    日本骨代謝学会学術集会プログラム抄録集(CD-ROM)　42nd　2024

    ISSN： 1349-0761

  3. 2種のサブセルラートランスクリプトームで硬組織を深掘り

    岡田寛之, 岡田寛之, 岡田寛之, 照井悠太, 関真秀, 鈴木絢子, 金井昭教, 瀬尾茂人, 寺島明日香, 小野寺晶子, 金澤三四朗, 矢野文子, 鍛治屋浩, 斎藤琢, GORI Francesca, 菊田順一, 鈴木穣, BARON Roland, 田中栄, 鄭雄一, 鄭雄一, 北條宏徳, 北條宏徳

    日本分子生物学会年会プログラム・要旨集(Web)　47th　2024

  4. Interventional and integrative subcellular sequencing (i2scseq) clarifies the multi differentiation stages embedded inside mature osteoclast

    Hiroyuki Okada, Yuta Terui, Yasunori Omata, Masahide Seki, Shoichiro Tani, Junya Miyahara, Kenta Makabe, Asuka Terashima, Sanshiro Kanazawa, Masahiro Hosonuma, Shoko Onodera, Fumiko Yano, Hiroyuki Kajiya, Taku Saito, Yutaka Suzuki, Koji Okabe, Roland Baron, Sakae Tanaka, Ung-il Chung, Hironori Hojo

    JOURNAL OF BONE AND MINERAL RESEARCH　38　52-53　2023/11

    ISSN： 0884-0431

    eISSN： 1523-4681

  5. Interventional and integrative subcellular sequencing (i2scseq) clarifies the multi differentiation stages embedded inside mature osteoclast

    Hiroyuki Okada, Yuta Terui, Yasunori Omata, Masahide Seki, Shoichiro Tani, Junya Miyahara, Kenta Makabe, Asuka Terashima, Sanshiro Kanazawa, Masahiro Hosonuma, Shoko Onodera, Fumiko Yano, Hiroyuki Kajiya, Taku Saito, Yutaka Suzuki, Koji Okabe, Roland Baron, Sakae Tanaka, Ung-il Chung, Hironori Hojo

    JOURNAL OF BONE AND MINERAL RESEARCH　38　334-335　2023/11

    ISSN： 0884-0431

    eISSN： 1523-4681

  6. Interventional and integrative subcellular sequencing (i2scseq) clarifies the multi differentiation stages embedded inside mature osteoclast

    Hiroyuki Okada, Yuta Terui, Yasunori Omata, Masahide Seki, Shoichiro Tani, Junya Miyahare, Kenta Makabe, Asuka Terashima, Sanshiro Kanazawa, Masahiro Hosonuma, Shoko Onodera, Fumiko Yano, Hiroyuki Kajiya, Taku Saito, Yutaka Suzuki, Koji Okabe, Roland Baron, Sakae Tanaka, Ung-Il Chung, Hironori Hojo

    JOURNAL OF BONE AND MINERAL RESEARCH　38　10-10　2023/11

    ISSN： 0884-0431

    eISSN： 1523-4681

  7. 頭頸部癌治療における再生医療の最前線 3Dプリンタを用いた組織・臓器構築

    疋田 温彦, 金澤 三四郎, 藤原 夕子, 鈴木 茂樹, 鄭 雄一, 星 和人, 高戸 毅

    頭頸部癌　42　(2)　135-135　2016/05

    Publisher: 日本頭頸部癌学会

    ISSN： 1349-5747

    eISSN： 1881-8382

  8. 人工臓器における3Dプリンターの応用 3Dプリンターによる外科用インプラントの創生と顎顔面領域への応用

    高戸 毅, 藤原 夕子, 金澤 三四朗, 菅野 勇樹, 鄭 雄一, 星 和人

    人工臓器　44　(2)　S-64　2015/10

    Publisher: (一社)日本人工臓器学会

    ISSN： 0300-0818

    eISSN： 1883-6097

  9. "医療機器"としての3Dプリンターの活用 その現状と将来 3Dプリンターによる外科用インプラントの創生

    高戸 毅, 藤原 夕子, 金澤 三四朗, 星 和人

    医療機器学　85　(2)　108-109　2015/04

    Publisher: (一社)日本医療機器学会

    ISSN： 1882-4978

    eISSN： 1884-054X

  10. 間葉-造血相互作用によるMSCの特性維持機構の評価

    金澤 三四朗, 馬渕 洋, 高戸 毅, 星 和人

    移植　49　(6)　440-440　2014/12

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

  11. 3Dプリンターが変える未来の医療 3Dプリンターによる外科用インプラントの創生

    高戸 毅, 藤原 夕子, 金澤 三四朗, 星 和人

    日本臨床外科学会雑誌　75　(増刊)　275-275　2014/10

    Publisher: 日本臨床外科学会

    DOI： 10.3919/jjsa.75.S275 　

    ISSN： 1345-2843

    eISSN： 1882-5133

  12. 3次元再生軟骨を培養・運搬するためのアガロースモールドの検討

    渡邊真, 金澤三四朗, 朴澤大樹, 森良之, 高戸毅, 星和人

    再生医療　12　2013

    ISSN： 1347-7919

  13. Research and development of implant-type tissue-engineered cartilage clinically applied for the nasal correction of the cleft lip and palate patients

    K. Hoshi, Y. Fujihara, Y. Asawa, S. Nishizawa, S. Kanazawa, T. Sakamoto, M. Watanabe, H. Saijo, Y. Mori, T. Takato

    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE　6　1-2　2012/09

    ISSN： 1932-6254

  14. 培養耳介軟骨細胞における基質産生の指標となるGFAPの分子生物学的な意義とそれを用いた品質管理法の確立

    金澤 三四朗, 星 和人, 西澤 悟, 永田 悟, 高戸 毅

    日本口腔科学会雑誌　61　(1)　118-118　2012/01

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

  15. 足場素材に対する液性免疫反応が軟骨再生に及ぼす影響

    金澤 三四朗, 藤原 夕子, 浅輪 幸世, 坂本 朋昭, 古村 眞, 高戸 毅, 星 和人

    移植　45　(3)　305-305　2010/06

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  16. 弾性軟骨におけるGFAPの機能(The functions of glial fibrillary acidic protein in the chondrocytes of elastic cartilage)

    金澤 三四朗, 西澤 悟, 高戸 毅, 星 和人

    日本細胞生物学会大会講演要旨集　62回　133-133　2010/05

    Publisher: (一社)日本細胞生物学会

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Books and Other Publications 2

  1. バイオ・医療への3Dプリンティング技術の開発最前線 = The frontiers and development of bio-medical 3D printing

    シーエムシー・リサーチ　2016/12

    ISBN: 9784904482322

  2. 再生医療用語ハンドブック

    岡野, 光夫, 浅島, 誠, 日本再生医療学会

    メディカルトリビューン　2015/03

    ISBN: 9784895894784

Presentations 34

  1. 病態メカニズム１

    日本再生医療学会総会　2026/03/20

  2. データ駆動研究現場の会 Invited

    日本再生医療学会総会　2026/03/19

  3. Elucidation of Risk Factors for Cleft Lip with Palate Through Advanced Transcriptomic Profiling Invited

    Sanshiro Kanazawa

    Lab Seminar for Washington University St. Louis　2025/09/03

  4. Expectations for exosome as a next generation therapy Invited

    Sanshiro Kanazawa

    2024/07/21

  5. シングルセルトランスクリプトミクス解析技術を 駆使した口唇口蓋裂リスク因子の特定

    金澤三四朗, 岡田寛之, 北條宏徳, 長村登紀子, 星和人

    第42回 日本骨代謝学会学術集会　2024/07/01

  6. ヒト臍帯由来間質細胞のシングルセル解析による口唇口蓋裂リスク因子の特定

    金澤三四朗, 岡田寛之, 北條宏徳, 長村登紀子, 星和人

    第23回 日本再生医療学会総会　2024/03/21

  7. ディープオミクスから眺める新たな生命現象 1生細胞内シーケンスで多核巨細胞の遺伝子発現制御に挑む

    岡田 寛之, 照井, 悠太, 小俣, 康徳, 関, 真秀 谷, 彰一郎, 宮原, 潤也, 真壁, 健太, 寺島, 明日香, 金澤, 三四朗, 細沼, 雅弘, 矢野, 文子, 小野寺, 晶子, 鍛治屋, 浩, 斎藤, 琢, 鈴木, 穣, 岡部, 幸司, Baron Rola, 田中 栄, 鄭, 雄一, 北條 宏徳

    第96回 日本生化学会大会　2023/10

  8. 運動器科学のCutting Edge 運動器発のサブセルラーシーケンスで、細胞生物学の常識に挑む

    岡田 寛之, 照井, 悠太, 小俣, 康徳, 関, 真秀 谷, 彰一郎, 宮原, 潤也, 真壁, 健太, 寺島, 明日香, 金澤, 三四朗, 細沼, 雅弘, 矢野, 文子, 小野寺, 晶子, 鍛治屋, 浩, 斎藤, 琢, 鈴木, 穣, 岡部, 幸司, Baron Rola, 田中 栄, 鄭, 雄一, 北條 宏徳

    第41回 日本骨代謝学会学術集会　2023/07

  9. ヒト臍帯組織の単一細胞トランスクリプトームプロファイリングによる口蓋裂リスク因子の特定

    金澤三四朗, 岡田寛之, 北條宏徳, 長村登紀子, 古村眞, 星和人

    第8回 日本骨免疫学会　2023/06/26

  10. 骨髄微小環境における間葉系間質細胞の転写およびエピゲノム解析による特性解明

    金澤三四朗, 西條英人, 星和人

    第67回 日本口腔外科学会総会　2022/11/05

  11. intra-single cell sequencing（iSC-seq）による破骨細胞の多核間制御メカニズム解明

    岡田寛之, 照井悠太, 小俣康徳, 関真秀, 谷彰一郎, 宮原潤也, 真壁健太, 寺島明日香, 金澤三四朗, 細沼雅弘, 矢野文子, 鍛治屋浩, 斎藤琢, 鈴木穣, 岡部幸司, Roland Baron, 田中栄, 鄭雄一, 北條宏徳

    NGS expo 2022　2022/10/18

  12. 造血-間葉相互作用による間葉系幹細胞の特性調節機序の解明

    金澤 三四朗, 岡田 寛之, 劉 丹, 馬渕 洋, 星 和人, 疋田 温彦

    第7回 日本骨免疫学会　2022/06/30

  13. Mesenchymal stromal cells in the bone marrow niche consist of multiple populations with distinct transcriptional and epigenetic properties

    Kanazawa S, Okada H, Hojo H, Ohba S, Iwata J, Komura M, Hikita A, Hoshi K

    ASBMR 2021 Annual Meeting　2021/10/02

  14. 骨髄ニッチを構成する間葉系間質細胞の転写およびエピジェネティック解析による特性解明 Invited

    金澤三四朗, 岡田寛之, 北條宏徳, 大庭伸介, 古村眞, 疋田温彦, 星和人

    第6回 日本骨免疫学会　2021/07/01

  15. Identification of Bone Marrow Mesenchymal Stromal Cell Population and Stem Cell Niche Signal

    Kanazawa S, Hojo H, Ohba S, Hikita A, Hoshi K

    ISSCR 2019　2019/06/20

  16. 間葉系幹細胞特性に影響を与える造血-間葉シグナルの同定

    金澤三四朗, 疋田温彦, 劉丹, 高戸毅, 星和人

    第16回日本再生医療学会総会　2017/03/08

  17. 3Dプリンターを用いた生体組織造形技術の開発 Invited

    金澤三四朗, 星和人, 高戸毅

    CMCリサーチセミナー　2016/12/15

  18. Analysis of Signals Involved in Stem Cell Property of MSC by Hematopoietic-Mesenchymal Interaction

    Kanazawa S, Hikita A, Takato T, Hoshi K

    13th ICRS World Congress　2016/09/25

  19. 3D Printer and Clinical Application Invited

    Sanshiro Kanazawa

    1st Northeast Aisa medical 3D print conference　2016/08/13

  20. Development of Surgical Implant Using 3D Printer and Clinical Application Invited

    Sanshiro Kanazawa, Kazuto Hoshi, Tsuyoshi Takato

    2016 Shanghai International 3D Printing & Intelligent Manufacturing Forum　2016/07/08

  21. Development of Surgical Implant Using 3D Printer and Clinical Application Invited

    Sanshiro Kanazawa, Kazuto Hoshi, Tsuyoshi Takato

    One-day Symposium on Advanced 3D Printing Technology and Innovative Applications　2015/09/29

  22. Analysis of Signal Involved in Maintenance of Stem Cell Property by Hematopoietic-Mesenchymal Interaction

    Kanazawa S, Takato T, Hoshi K

    12th World Congress of International Cartilage Repair Society　2015/05/09

  23. 造血‐間葉相互作用によるMSCの特性維持に関わるシグナル解析

    金澤三四朗, 高戸毅, 星和人

    第14回日本再生医療学会総会　2015/03/21

  24. 間葉‐造血相互作用によるMSCの特性維持機構の評価

    金澤三四朗, 馬渕洋, 高戸毅, 星和人

    第33回日本運動器移植・再生医学研究会　2014/09/27

  25. Maintenance of Stem-ness in Mesencymal Stem Cells by mesenchymal-hematopoietic interaction

    Kanazawa S, Mabuchi Y, Akazawa C, Takato T, Hoshi K

    12th International Society for Stem Cell Research　2014/06/19

  26. 間葉‐造血相互作用により増殖したMSCの軟骨分化誘導後の成熟評価

    金澤三四朗, 馬渕洋, 正本庸介, 荒井俊也, 高戸毅, 星和人

    第13回日本再生医療学会総会　2014/03/05

  27. Evaluation on chondrogenic maturity of proliferated MSC by mesenchymal-hematopoietic interaction

    Kanazawa S, Takato T, Hoshi K

    11th World Congress of International Cartilage Repair Society　2013/09/18

  28. Biological Roles of Glial Fibrillary Acidic Protein as a Biomarker in Cartilage Regenerative Medicine

    Kanazawa K, Takato T, Hoshi K

    Frontiers 2013 EPFL Symposium　2013/06/21

  29. 造血・間葉相互作用を用いたMSCの増殖促進の検討

    金澤三四朗, 高戸毅, 星和人

    第12回日本再生医療学会総会　2013/03/22

  30. 任意形状設計により作製したbox-type β-TCP/PLGC複合体を用いた再生軟骨の成熟度評価

    金澤三四朗, 菊池正紀, 狩生景基, 高戸毅, 星和人

    第12回日本再生医療学会総会　2013/03/22

  31. 軟骨再生医療に用いる培養耳介軟骨細胞の指標となるGFAPの分子生物学的な意義と品質管理法の確立

    金澤三四朗, 西澤悟, 高戸毅, 星和人

    第21回日本形成外科学会基礎学術集会　2012/10/04

  32. Biological Roles of Glial Fibrillary Acidic Protein as a Biomarker Indicating Matrix Production of Cultured Chondrocytes in Cartilage Regenerative Medicine

    Kanazawa S, Takato T, Hoshi K

    2th TERMIS World Congress　2012/09/07

  33. 軟骨基質産生の指標となりうるグリア線維性酸性タンパクの耳介軟骨細胞における役割

    金澤三四朗, 西澤悟, 高戸毅, 星和人

    第10回日本再生医療学会　2011/03/01

  34. Glial Fibrillary Acidic Protein Maintains Nuclear Morphology and Supports the Resistance to Severe Mechanical Stress in the Chondrocytes of Elastic Cartilage

    Kanazawa S, Nishizawa S, Takato T, Hoshi K

    9st world congress of International Cartilage Repair Society　2010/09/26

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Industrial Property Rights 1

  1. 骨膜および骨再生用組成物

    金澤三四朗, 竹本国夫, 星和人

    Property Type: Patent

Research Projects 9

  1. エンハンサー配列変異が神経堤細胞特性に与える影響と顎顔面形態形成機構の統合的理解

    金澤三四朗

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 国際共同研究加速基金

    Institution: 東京大学医学部附属病院

    2026/04 - 2028/03

  2. 骨折予後改善のための臨床リアルワールドエビデンスと成熟破骨細胞治療ターゲット探索

    岡田寛之, 金澤三四朗

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究（C)

    Institution: 東京大学医学部附属病院

    2025/04 - 2027/03

  3. ヒト臍帯組織のエピゲノム解析による間葉系細胞の分化指向の立証と骨再生医療への展開

    金澤三四朗 岡田寛之 北條宏徳 長村登紀子 成田理香

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究（C)

    Institution: 東京大学医学部附属病院

    2025/04 - 2027/03

  4. 慢性歯周炎に対する抗炎症作用と組織再生を目的とした溶解型マイクロニードルを用いたエクソソーム投与技術の開発

    金澤三四朗

    Offer Organization: 国立研究開発法人日本医療研究開発機構（AMED）

    System: 橋渡し研究プログラム

    Institution: 東京大学医学部附属病院

    2025/04 - 2027/03

  5. Evaluation of properties by epigenome analysis for human umbilical cord-derived stromal cells and research for bone regenerative medicine

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: The University of Tokyo

    2022/04 - 2025/03

  6. Elucidation of stem cell signal using genome structure as an index and application to bone regenerative medicine

    Hoshi Kazuto

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: The University of Tokyo

    2018/04 - 2021/03

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    One of the important cell lines derived from bone marrow is the MSC. In recent years, markers of MSC isolation have been reported, but they are still heterogeneous cell populations. Therefore, it is difficult to accurately evaluate the MSC characteristics. In this study, scRNA-seq analysis was used to identify seven genetically distinct subpopulations of mouse bone marrow MSCs. ATAC-seq analysis also revealed epigenetic landscapes, genetic similarities and functional characteristics. These results elucidate stem cell niche signals in the bone marrow microenvironment, reconstitute bone marrow in vitro, and are potentially important for subpopulations identified in various clinical applications in the treatment of bone and bone marrow-related disorders. It made it possible to shed light on the role.

  7. Elucidation of bone marrow niche signal using epigenome analysis and establishment of bone marrow reconstitution culture method

    Kanazawa Sanshiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Research Activity Start-up

    2019/04 - 2020/03

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    The author examined to isolate mesenchymal cell populations by analyzing MSCs at the single cell level. The MSCs were analyzed by scRNA-Seq, and seven specific clusters were confirmed from the gene expression profile. In addition, each cluster were confirmed that they could be separated using FACS from the gene expression profile. Furthermore, author revealed that seven clusters were independent cell populations by RNA-Seq and ATAC-seq analysis. Next, author performed in vitro characterization of these clusters. As a result, aouthor found that some of the clusters had a function closely stem cells. Author aim to identify stem cell-like cell populations by direct comparison with ES cells in the future.

  8. Elucidation of mesenchymal stem cell maintenance mechanism by hematopoietic-mesenchymal interaction and application of bone regenerative medicine

    SUGIYAMA Madoka

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: The University of Tokyo

    2016/04 - 2019/03

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    In this study, we will elucidate stemness signals of MSCs induced by hematopoietic stem cells and aim to establish a method to achieve the promotion and maintenance of bone formation by utilizing signal related molecules in recombinant human (rh) BMP-2 induced regenerated bone. In addition, we aim at application to bone regeneration by realizing functional bone regeneration that can maintain bone volume and bone quality for a long time. Eventually, we studied with the aim of contributory to develop oral surgery and regenerative medicine. From the results of in vivo and in vitro studies, we indicated in order to maintain stem cell properties, mobilize and establish bone marrow cells, persistent release of stemness factor is required. Therefore, we suggest that be necessary to study the continuing release of the factor in the future.

  9. Fundamental study on bone regeneration by allogeneic adipose-derived stem cells utilizing immunotolerance of stem cells

    Takahashi Naoko, TAKATO Tshuyoshi, HOSHI Kazuto, KANAZAWA Sanshiro, UTO Sakura

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: The University of Tokyo

    2016/04/01 - 2018/03/31

    More details Close

    We examined the factors associated in immune tolerance by cytokine assay. However, ASC isolated by FACS was remained an issue, as these cells were heterogeneity cell population. Therefore, we verified whether there are cells involved in immune tolerance in the population using single cell RNA-Seq. As a result, ASC isolated by the conventionally method revealed that were consists of ten populations from genetic similarity. Furthermore, through global gene analysis, expression of a genes related in an immune tolerance were upregulated in a specific cell population X. When this cell population was co-cultured with CD3-positive T cells in LPS-supplemented medium, we confirmed differentiation of CD3 positive T cells to CD4/25/Foxp3 positive Treg was significantly increased. In addition, the signal existing between population X and CD3 positive T cells was analyzed by RNA-Seq. As a result, we identified candidate factors associated in the immune tolerance.

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Teaching Experience 1

  1. クリニカルクラークシップ（歯学概論）　東京大学医学部 Undergraduate (specialized)

Media Coverage 1

  1. origin of future

    2015/06

    Type: TV or radio program