Details of the Researcher

PHOTO

Keishi Suga
Section
Graduate School of Engineering
Job title
Associate Professor
Degree

  • Ph. D. (Osaka University)

e-Rad No.
00709800

Research History 2

  • 2020/04 - Present
    Tohoku University　Graduate School of Engineering

  • 2013/04 - 2020/03
    Osaka University　Graduate School of Engineering Science　Assistant Professor

Education 3

  • Osaka University　Graduate School of Engineering Science　Division of Chemical Engineering

    2010/04 - 2013/03

  • Osaka University　Graduate School of Engineering Science　Division of Chemical Engineering

    2008/04 - 2010/03

  • Osaka University　School of Engineering Science

    2004/04 - 2008/03

Committee Memberships 4

  • 日本膜学会　編集委員

    2024/06 - Present

  • 化学工学会東北支部　幹事

    2023/04 - Present

  • 分離技術会　総務委員

    2018/04 - Present

  • 化学工学会バイオ部会　庶務幹事

    2018/04 - 2022/03

Professional Memberships 4

  • 分離技術会

  • 日本膜学会

  • 日本化学会

  • 化学工学会

Research Areas 3

  • Nanotechnology/Materials / Composite materials and interfaces /

  • Nanotechnology/Materials / Thin-film surfaces and interfaces /

  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering /

Awards 5

  1. 2024年度膜学研究奨励賞

    2024/06　日本膜学会　脂質膜界面評価を基盤とするバイオインターフェイスの設計に関する研究

  2. 膜誌論文賞（2020年発行）

    2021/06　Silver Nanoparticle–Phospholipid Self–Assembly Systems for Membrane Surface–Enhanced Raman Spectroscopy Analysis

  3. H30年度化学工学会研究奨励賞

    2019/03　化学工学会　Cubosomalゲルの作製・評価方法の開発とその応用に関する研究

  4. 若手口頭講演賞

    2017/10　日本化学会コロイドおよび界面化学部会　膜表面増強ラマン分光法の開発～金ナノ粒子を活用する高感度脂質膜分析～

  5. 優秀講演賞（学術）

    2017/04　日本化学会　Multi-Focal Characterization for Micro-Phase Separation Behavior of Liposome Membrane

Papers 109

  1. A Quantitative Approach to Characterize the Surface Modification on Nanoparticles Based on Localized Dielectric Environments Peer-reviewed

    Taketo Mochizuki, Shota Sampei, Keishi Suga, Kanako Watanabe, Tom A. J. Welling, Daisuke Nagao

    Analytical Chemistry　2024/02/27

    DOI： 10.1021/acs.analchem.3c03593 　

  2. Gel-Phase-like Ordered Membrane Properties Observed in Dispersed Oleic Acid/1-Oleoylglycerol Self-Assemblies: Systematic Characterization Using Raman Spectroscopy and a Laurdan Fluorescent Probe Peer-reviewed

    Keishi Suga, Yoko Otsuka, Yukihiro Okamoto, Hiroshi Umakoshi

    Langmuir　34　(5)　2081-2088　2018/02/06

    DOI： 10.1021/acs.langmuir.7b04044 　

    ISSN： 1520-5827 0743-7463

    eISSN： 1520-5827

  3. Interaction forces and membrane charge tunability: Oleic acid containing membranes in different pH conditions Peer-reviewed

    James Kurniawan, Keishi Suga, Tonya L. Kuhl

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES　1859　(2)　211-217　2017/02

    DOI： 10.1016/j.bbamem.2016.11.001 　

    ISSN： 0005-2736

    eISSN： 0006-3002

  4. Membrane Surface-Enhanced Raman Spectroscopy for Sensitive Detection of Molecular Behavior of Lipid Assemblies Peer-reviewed

    Keishi Suga, Tomohiro Yoshida, Haruyuki Ishii, Yukihiro Okamoto, Daisuke Nagao, Mikio Konno, Hiroshi Umakoshi

    ANALYTICAL CHEMISTRY　87　(9)　4772-4780　2015/05

    DOI： 10.1021/ac5048532 　

    ISSN： 0003-2700

    eISSN： 1520-6882

  5. Detection of Nanosized Ordered Domains in DOPC/DPPC and DOPC/Ch Binary Lipid Mixture Systems of Large Unilamellar Vesicles Using a TEMPO Quenching Method Peer-reviewed

    Keishi Suga, Hiroshi Umakoshi

    LANGMUIR　29　(15)　4830-4838　2013/04

    DOI： 10.1021/la304768f 　

    ISSN： 0743-7463

  6. Surface Modification of Silica-Supported Supraparticles to Control Their Optical Properties and Mobility in Electric Fields

    Keisuke Kurioka, Natsuho Tsunetomi, Hikaru Namigata, Keishi Suga, Kanako Watanabe, Daisuke Nagao, Tom A. J. Welling

    Langmuir　2026/06/16

    DOI： 10.1021/acs.langmuir.6c00880 　

  7. Designing hollow-structured nanoreactors for effective use of catalytic nanoparticles by balancing mass transport and reaction kinetics

    Hana Aizawa, Tom A.J. Welling, Shin Saito, Hikaru Namigata, Keishi Suga, Kanako Watanabe, Daisuke Nagao

    Chemical Engineering Journal　536　175913-175913　2026/05

    Publisher: Elsevier BV

    DOI： 10.1016/j.cej.2026.175913 　

    ISSN： 1385-8947

  8. Moderately clustered gold nanoparticles preserved in in-situ polymerized hydrogels towards facile SERS sensing

    Hikaru Namigata, Kanako Watanabe, Tom A. J. Welling, Keishi Suga, Daisuke Nagao

    COLLOID AND POLYMER SCIENCE　2026/01/20

    DOI： 10.1007/s00396-026-05568-y 　

    ISSN： 0303-402X

    eISSN： 1435-1536

  9. Surface-Enhanced Raman Scattering in Au Nanostructures with Submolecular Gaps for Molecular Size Detection

    Nobutomo Nakamura, Nozomi Morishita Watanabe, Karin Hattori, Kaichi Yatsugi, Keishi Suga

    ACS Omega　2025/12/23

    DOI： 10.1021/acsomega.5c09293 　

  10. Molecular Enrichment in the Cavity of Hollow Mesoporous Silica Particles: the Effect of the Cavity Size

    Shin Saito, Hana Aizawa, Kanako Watanabe, Tom A. J. Welling, Hikaru Namigata, Keishi Suga, Daisuke Nagao

    Particle & Particle Systems Characterization　2025/11/23

    Publisher: Wiley

    DOI： 10.1002/ppsc.202500188 　

    ISSN： 0934-0866

    eISSN： 1521-4117

    More details Close

    ABSTRACT Hollow particles have attracted attention in various applications such as adsorbents and catalytic supports owing to their unique functions derived from their morphology. Molecules are reported to be concentrated within hollow cavities (i.e., molecular enrichment), which improves the performance of functional materials such as adsorbents and catalysts. However, the concentration of molecules localized within hollow cavities has not been experimentally quantified so far. In the present work, the enrichment of rhodamine B (RhB) in the cavities of hollow mesoporous silica particles (Hollow m SiO 2 ) is quantitatively demonstrated. Three different types of particles (spherical, core‐shell, and hollow particles) with comparable sizes are prepared to estimate the molecular concentration in the hollow cavity. Through adsorption experiments of RhB with each particle, it is revealed that the RhB concentration in the hollow cavity is much higher than in the bulk solutions. The enrichment is also found to be enhanced with a decrease in cavity size. The quantitative methodology of molecular concentration in hollow cavities is applicable to other molecules such as reactants for catalytic reactions. This reveals the impact of the enrichment effect on the performance of various applications, achieving appropriate design of hollow‐structured materials.

  11. Effects of Mesoporous Silica Particle Loading on the Properties of PDMS-Based Mixed Matrix Membrane

    Tomoya Yoshihara, Keishi Suga, Hikaru Namigata, Kanako Watanabe, Tom A.J. Welling, Kumiko Hayashi, Lester C. Geonzon, Koichi Mayumi, Daisuke Nagao

    Chemical Engineering Journal Advances　100803-100803　2025/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.ceja.2025.100803 　

    ISSN： 2666-8211

  12. Visualization of the packing structure of packed beds with micron-sized particles using confocal microscopy to estimate their pressure drop

    Hiroki Nishida, Tom A.J. Welling, Hikaru Namigata, Kousuke Hiromori, Keishi Suga, Daisuke Nagao, Kanako Watanabe

    Chemical Engineering Journal　515　163519-163519　2025/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.cej.2025.163519 　

    ISSN： 1385-8947

  13. Supraparticles with Tailored Absorption and Their Optical Performance as Photonic Pigments in Sustainable Water-Based Paint

    Tom A. J. Welling, Keisuke Kurioka, Gantulga Tuguldur, Natsuho Tsunetomi, Hikaru Namigata, Keishi Suga, Kanako Watanabe, Daisuke Nagao

    ACS Applied Optical Materials　2025/04/25

    DOI： 10.1021/acsaom.5c00080 　

  14. Amyloid beta (Aβ) fibrillation kinetics and its impact on membrane polarity. International-journal

    Arun Ajaikumar, Nozomi Morishita Watanabe, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    Journal of bioenergetics and biomembranes　57　(1)　1-10　2025/02

    DOI： 10.1007/s10863-024-10046-7 　

    More details Close

    Fibrillation of the amyloid beta (Aβ) peptide has often been associated with neurodegenerative pathologies such as Alzheimer's disease. In this study we examined the influence of several potential compositions of the lipid membrane on Aβ fibrillation by using liposomes as a basic model membrane. Firstly, it was revealed that Aβ fibrillation kinetics were enhanced and had the potential to occur at a faster rate on more fluid membranes compared to solid membranes. Next, the extent of fibril-related damage to membranes was examined with analysis of membrane polarity via the steady-state emission spectra of 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan). It was revealed that there was slight hydration behavior of the membrane during the lag phase (tlag) of the kinetic process, possibly coinciding with Aβ monomer binding. However, as the fibrillation kinetic process continued the membrane gradually dehydrated. Hydration states of membranes during and after Aβ fibrillation processes were further examined via deconvolution analysis of the obtained Laurdan spectra. This allows a mapping of membrane hydration from the interior to exterior regions of the lipid membrane. Results revealed slight but definitive variations in deeper region membrane polarity during the time course of Aβ fibrillation, suggesting Aβ aggregation impacts not only the surface level aggregating region but also the inner regions of the membrane. These results can ultimately contribute to the future investigations of the nature of the membrane damage caused by Aβ aggregation.

  15. Quantitative Characterization of Localized Hydrophobicity Surrounding Polymer Particle Surfaces for Aqueous Antibacterial Colloids

    Akira Nagasawa, Kensuke Fujishima, Keishi Suga, Kanako Watanabe, Daisuke Nagao

    Langmuir　2025/01/14

    DOI： 10.1021/acs.langmuir.4c03896 　

  16. A practical process for effective thickening of silica shells formed onto spherical cores by considering the variation in ionic strength during sol-gel reaction Peer-reviewed

    Kanako Watanabe, Ryuto Fujinuma, Hana Aizawa, Hikaru Namigata, Tom A.J. Welling, Keishi Suga, Daisuke Nagao

    Microporous and Mesoporous Materials　379　113274-113274　2024/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.micromeso.2024.113274 　

    ISSN： 1387-1811

  17. Colloidal photonic crystals with tunable reflection wavelengths or intensities derived from their reconfigurable structures Peer-reviewed

    Hikaru Namigata, Kanako Watanabe, Tom A.J. Welling, Keishi Suga, Daisuke Nagao

    Colloid and Interface Science Communications　62　100806-100806　2024/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.colcom.2024.100806 　

    ISSN： 2215-0382

  18. Bio‐Inspired Polydiacetylene Vesicles for Controlling Stimulus Sensitivity Peer-reviewed

    Shota Nakayama, Keishi Suga, Tatsuya Kamata, Kanako Watanabe, Hikaru Namigata, Tom A. J. Welling, Daisuke Nagao

    Macromolecular Reaction Engineering　2024/07/29

    Publisher: Wiley

    DOI： 10.1002/mren.202400016 　

    ISSN： 1862-832X

    eISSN： 1862-8338

    More details Close

    Abstract Polydiacetylene (PDA) is a kind of photopolymerizable polymer, which exhibits a unique color transition in response to external stimuli such as heat, pH, and solvent. PDAs are attractive as eye‐detection stimulus sensors with excellent time performance; however, the sensitivity of PDAs should be improved. Considering the biological membrane‐like structure of diacetylene (DA) vesicles, their modification by incorporating membrane lipids (e.g., diacylphosphocholine, PC) can be used to control the membrane fluidity, and consequently molecular ordering of DAs in the vesicle. Inspired by biological membrane systems, lipid vesicles are employed as platforms to generate PDA, and essential factors that influence the sensitivity of PDA are investigated. By lowering the polymerization temperature, the generation of PDA becomes slower, while the sensitivity improves. By adding PCs at the molar ratio of lipid:DA = 1:1, the sensitivity of PDA can be varied: the PCs with lower phase transition temperatures (Tm) made PDA insensitive, while the PCs with higher Tm improved the sensitivity as compared to pure poly(PCDA). It is concluded that the photopolymerization of DAs with a lower membrane fluidity induces highly sensitive PDA, while the photopolymerization of DAs with a higher membrane fluidity induces insensitive PDA with robustness toward stimuli.

  19. Double-inverse-opal structured films of a hydrogel framework and mobile inorganic particles Peer-reviewed

    Hikaru Namigata, Kanako Watanabe, Tom A.J. Welling, Keishi Suga, Daisuke Nagao

    Colloids and Surfaces A: Physicochemical and Engineering Aspects　690　133781-133781　2024/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.colsurfa.2024.133781 　

    ISSN： 0927-7757

  20. Switchable Bragg Reflections via Controllable Inner Particle Motion in Yolk–Shell Colloidal Crystals Peer-reviewed

    Hikaru Namigata, Tom A. J. Welling, Kanako Watanabe, Keishi Suga, Arnout Imhof, Alfons van Blaaderen, Daisuke Nagao

    ACS Applied Optical Materials　2023/12/20

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsaom.3c00392 　

    ISSN： 2771-9855

    eISSN： 2771-9855

  21. Highly Reflective and Transparent Shell-Index-Matched Colloidal Crystals of Core–Shell Particles for Stacked RGB Films Peer-reviewed

    Tom A.J. Welling, Keisuke Kurioka, Hikaru Namigata, Keishi Suga, Daisuke Nagao, Kanako Watanabe

    ACS Applied Nano Materials　2023/11/07

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsanm.3c03940 　

    ISSN： 2574-0970

    eISSN： 2574-0970

  22. Dispersibility and surface properties of hydrocortisone-incorporated self-assemblies Peer-reviewed

    Ward Wakileh, Nozomi Watanabe, Keishi Suga, Naoki Ikushima, Naoko Kajimura, Kaoru Mitsuoka, Yukihiro Okamoto, Hiroshi Umakoshi

    Colloids and Surfaces A: Physicochemical and Engineering Aspects　665　131217-131217　2023/05

    Publisher: Elsevier BV

    DOI： 10.1016/j.colsurfa.2023.131217 　

    ISSN： 0927-7757

  23. Surface lattice resonance in three-dimensional plasmonic arrays fabricated via self-assembly of silica-coated gold nanoparticles Peer-reviewed

    Masashi Hasegawa, Kanako Watanabe, Hikaru Namigata, Tom A.J. Welling, Keishi Suga, Daisuke Nagao

    Journal of Colloid and Interface Science　633　226-232　2023/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.jcis.2022.11.077 　

    ISSN： 0021-9797

  24. Intracellular Magnetic Control over the Reversible Assembly of Silica-Based Magnetic Composite Particles for Photothermal Therapy Peer-reviewed

    Ryota Kameda, Taisei Suzuki, Ikumi Kato, Keishi Suga, Kanako Watanabe, Kimiko Nagino, Kumiko Hayashi, Satoshi Arai, Daisuke Nagao

    ACS Applied Nano Materials　2023/02/23

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsanm.2c05550 　

    ISSN： 2574-0970

    eISSN： 2574-0970

  25. Independent control over sizes and surface properties of polystyrene-based particles using multiple comonomers Peer-reviewed

    Akira Nagasawa, Kanako Watanabe, Keishi Suga, Daisuke Nagao

    Colloids and Surfaces A: Physicochemical and Engineering Aspects　656　130376-130376　2023/01

    Publisher: Elsevier BV

    DOI： 10.1016/j.colsurfa.2022.130376 　

    ISSN： 0927-7757

  26. Fluorescence Spectroscopic Analysis of Lateral and Transbilayer Fluidity of Exosome Membranes Peer-reviewed

    Tomokazu Yasuda, Hirofumi Watanabe, Koichiro M. Hirosawa, Kenichi G. N. Suzuki, Keishi Suga, Shinya Hanashima

    Langmuir　2022/11/24

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.langmuir.2c02258 　

    ISSN： 0743-7463

    eISSN： 1520-5827

  27. Alternative fouling analysis of PVDF UF membrane for surface water treatment: The credibility of silver nanoparticles Peer-reviewed

    Diyana Kamarudin, N. Awanis Hashim, Boon Hoong Ong, Miftah Faried, Keishi Suga, Hiroshi Umakoshi, Wan Adibah Wan Mahari

    Journal of Membrane Science　661　120865-120865　2022/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.memsci.2022.120865 　

    ISSN： 0376-7388

  28. Preferential adsorption of L-tryptophan by L-phospholipid coated porous polymer particles Peer-reviewed

    Hayato Takase, Keishi Suga, Hideki Matsune, Hiroshi Umakoshi, Koichiro Shiomori

    Colloids and Surfaces B: Biointerfaces　216　112535-112535　2022/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.colsurfb.2022.112535 　

    ISSN： 0927-7765

  29. Dependence of the Core–Shell Structure on the Lipid Composition of Nanostructured Lipid Carriers: Implications for Drug Carrier Design Peer-reviewed

    Ni’matul Izza, Nozomi Watanabe, Yukihiro Okamoto, Keishi Suga, Yusuf Wibisono, Naoko Kajimura, Kaoru Mitsuoka, Hiroshi Umakoshi

    ACS Applied Nano Materials　5　(7)　9958-9969　2022/07/22

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsanm.2c02214 　

    ISSN： 2574-0970

    eISSN： 2574-0970

  30. Surface Characteristics of Antibacterial Polystyrene Nanoparticles Synthesized Using Cationic Initiator and Comonomers Peer-reviewed

    Keishi Suga, Makina Murakami, Shota Nakayama, Kanako Watanabe, Sayuri Yamada, Toshikazu Tsuji, Daisuke Nagao

    ACS Applied Bio Materials　5　(5)　2202-2211　2022/05/16

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsabm.2c00046 　

    ISSN： 2576-6422

    eISSN： 2576-6422

  31. Preparation of Planar Lipid Bilayer Membrane by Utilizing Bicelles and Its Characterization Peer-reviewed

    Shogo Taguchi, Yukihiro Okamoto, Keishi Suga, Ho Sup Jung, Hiroshi Umakoshi

    Kagaku Kogaku Ronbunshu　48　(5)　175-181　2022

    Publisher: Society of Chemical Engineers, Japan

    DOI： 10.1252/kakoronbunshu.48.175 　

    ISSN： 0386-216X

    eISSN： 1349-9203

  32. Insight into the Exosomal Membrane: From Viewpoints of Membrane Fluidity and Polarity Peer-reviewed

    Keishi Suga, Daiki Matsui, Nozomi Watanabe, Yukihiro Okamoto, Hiroshi Umakoshi

    Langmuir　37　(38)　11195-11202　2021/09/28

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.langmuir.1c00687 　

    ISSN： 0743-7463

    eISSN： 1520-5827

  33. Correlation of Secondary Particle Number with the Debye–Hückel Parameter for Thickening Mesoporous Silica Shells Formed on Spherical Cores Peer-reviewed

    Kota Fujimoto, Shunho Ishikawa, Kanako Watanabe, Haruyuki Ishii, Keishi Suga, Daisuke Nagao

    ACS Omega　6　(27)　17734-17740　2021/07/13

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsomega.1c02293 　

    ISSN： 2470-1343

    eISSN： 2470-1343

  34. Modulation of the Belousov–Zhabotinsky Reaction with Lipid Bilayers: Effects of Lipid Head Groups and Membrane Properties Peer-reviewed

    Michael S. Chern, Nozomi Watanabe, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    Langmuir　37　(22)　6811-6818　2021/06/08

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.langmuir.1c00915 　

    ISSN： 0743-7463

    eISSN： 1520-5827

  35. Systematic Characterization of Nanostructured Lipid Carriers from Cetyl Palmitate/Caprylic Triglyceride/Tween 80 Mixtures in an Aqueous Environment Peer-reviewed

    Ni'Matul Izza, Keishi Suga, Yukihiro Okamoto, Nozomi Watanabe, Tham Thi Bui, Yusuf Wibisono, Cut Rifda Fadila, Hiroshi Umakoshi

    Langmuir　37　(14)　4284-4293　2021/04

    DOI： 10.1021/acs.langmuir.1c00270 　

    ISSN： 0743-7463

    eISSN： 1520-5827

  36. Pore expanding effect of hydrophobic agent on 100 nm-sized mesoporous silica particles estimated based on Hansen solubility parameters Peer-reviewed

    Kota Fujimoto, Kanako Watanabe, Shunho Ishikawa, Haruyuki Ishii, Keishi Suga, Daisuke Nagao

    Colloids and Surfaces A: Physicochemical and Engineering Aspects　609　125647　2021/01/20

    DOI： 10.1016/j.colsurfa.2020.125647 　

    ISSN： 0927-7757

    eISSN： 1873-4359

  37. Double-inverse-opal-structured particle assembly as a novel immobilized photocatalytic material Peer-reviewed

    Hikaru Namigata, Kanako Watanabe, Saya Okubo, Masashi Hasegawa, Keishi Suga, Daisuke Nagao

    Materials　14　(1)　1-9　2021/01/01

    DOI： 10.3390/ma14010028 　

    eISSN： 1996-1944

  38. Structure and properties characterization of amphiphilic dendrons modified lipid membrane Peer-reviewed

    Jin Han, Yosuke Iimure, Yukihiro Okamoto, Keishi Suga, Hiroshi Umakoshi

    Chemistry Letters　50　(1)　187-190　2021

    DOI： 10.1246/CL.200633 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  39. Investigation of Quercetin interaction behaviors with lipid bilayers: Toward understanding its antioxidative effect within biomembrane Peer-reviewed

    Jin Han, Misaki Amau, Yukihiro Okamoto, Keishi Suga, Hiroshi Umakoshi

    Journal of Bioscience and Bioengineering　132　(1)　49-55　2021

    DOI： 10.1016/j.jbiosc.2021.03.004 　

    ISSN： 1389-1723

    eISSN： 1347-4421

  40. Characterization of pH-Responsive Self-Assembly Behaviors of Fatty Acid-Functionalized Prodrug Peer-reviewed

    Jin Han, Keita Hayashi, Yukihiro Okamoto, Keishi Suga, Hiroshi Umakoshi

    Biochemical Engineering Journal　164　107794　2020/12/15

    DOI： 10.1016/j.bej.2020.107794 　

    ISSN： 1369-703X

    eISSN： 1873-295X

  41. Changes Caused by Liposomes to the Belousov-Zhabotinsky Reaction Peer-reviewed

    Michael S. Chern, Yukihiro Okamoto, Keishi Suga, Nozomi Watanabe, Hiroshi Umakoshi

    Journal of Physical Chemistry B　124　(44)　9862-9869　2020/11/05

    DOI： 10.1021/acs.jpcb.0c06413 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  42. A novel method of vesicle preparation by simple dilution of bicelle solution Peer-reviewed

    Shogo Taguchi, Bong-Su Kang, Keishi Suga, Yukihiro Okamoto, Ho-Sup Jung, Hiroshi Umakoshi

    Biochemical Engineering Journal　162　107725-107725　2020/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.bej.2020.107725 　

    ISSN： 1369-703X

    eISSN： 1873-295X

  43. Site Specific Analysis of Anionic Lipid by Membrane Surface-enhanced Raman Spectroscopy with Different Sized Gold Nanoparticles Peer-reviewed

    Miftah Faried, Seiya Ando, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    Chemistry Letters　49　(9)　1107-1110　2020/09/05

    Publisher: The Chemical Society of Japan

    DOI： 10.1246/cl.200389 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  44. Enzymatic hydrolysis of cellulose recovered from ionic liquid-salt aqueous two-phase system Peer-reviewed

    Kazuhiko Tanimura, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    Journal of Bioscience and Bioengineering　129　(5)　624-631　2020/05

    Publisher: Elsevier BV

    DOI： 10.1016/j.jbiosc.2019.10.012 　

    ISSN： 1389-1723

    eISSN： 1347-4421

  45. Evaluation of Molecular Ordering in Bicelle Bilayer Membranes Based on Induced Circular Dichroism Spectra Peer-reviewed

    Keishi Suga, Kazuki Kitagawa, Shogo Taguchi, Yukihiro Okamoto, Hiroshi Umakoshi

    Langmuir　36　(12)　3242-3250　2020/03/31

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.langmuir.9b03710 　

    ISSN： 0743-7463

    eISSN： 1520-5827

  46. Silver Nanoparticle–Phospholipid Self–Assembly Systems for Membrane Surface– Enhanced Raman Spectroscopy Analysis Peer-reviewed

    Faried Miftah, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    MEMBRANE　45　(4)　187-192　2020

    Publisher: The Membrane Society of Japan

    DOI： 10.5360/membrane.45.187 　

    ISSN： 0385-1036

    eISSN： 1884-6440

  47. Membrane Surface-Enhanced Raman Spectroscopy for Cholesterol-Modified Lipid Systems: Effect of Gold Nanoparticle Size Peer-reviewed

    Miftah Faried, Keishi Suga, Yukihiro Okamoto, Kamyar Shameli, Mikio Miyake, Hiroshi Umakoshi

    ACS Omega　4　(9)　13687-13695　2019/08

    Publisher:

    DOI： 10.1021/acsomega.9b01073 　

    eISSN： 2470-1343

  48. Ergosterol-Induced Ordered Phase in Ternary Lipid Mixture Systems of Unsaturated and Saturated Phospholipid Membranes Peer-reviewed

    Tham Thi Bui, Suga Keishi, Umakoshi Hiroshi

    JOURNAL OF PHYSICAL CHEMISTRY B　123　(29)　6161-6168　2019/07/25

    DOI： 10.1021/acs.jpcb.9b03413 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  49. Melting Temperature Dependent Interactions of Ergosterol with Unsaturated and Saturated Lipids in Model Membranes Peer-reviewed

    Tham Thi Bui, Keishi Suga, Tonya L. Kuhl, Hiroshi Umakoshi

    Langmuir　35　(32)　10640-10647　2019/07

    Publisher:

    DOI： 10.1021/acs.langmuir.9b01538 　

    ISSN： 0743-7463

    eISSN： 1520-5827

  50. Potential Interaction Behavior of Lanosterol and Unsaturated Phosphocholine in Monolayer Membrane Peer-reviewed

    Tham Thi Bui, Keishi Suga, Hiroshi Umakoshi

    Membrane　44　199-203　2019/07

    Publisher:

    DOI： 10.5360/membrane.44.199 　

  51. Characterization of Ionic Liquid Aqueous Two-Phase Systems: Phase Separation Behaviors and the Hydrophobicity Index between the Two Phasese Peer-reviewed

    Kazuhiko Tanimura, Misaki Amau, Ryosuke Kume, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    J. Phys. Chem. B　123　(27)　5866-5874　2019/06

    Publisher:

    DOI： 10.1021/acs.jpcb.9b04848 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  52. Lipid-Surrounding Water Molecules Probed by Time-Resolved Emission Spectra of Laurdan. International-journal Peer-reviewed

    Nozomi Watanabe, Keishi Suga, J Peter Slotte, Thomas K M Nyholm, Hiroshi Umakoshi

    Langmuir : the ACS journal of surfaces and colloids　35　(20)　6762-6770　2019/05/21

    DOI： 10.1021/acs.langmuir.9b00303 　

    More details Close

    The hydration states of the interfacial region of lipid bilayers were investigated on the basis of the time-resolved emission spectra (TRES) analysis of 6-lauroyl-2-dimethylamino naphthalene (Laurdan), a common fluorescence probe used to analyze membrane hydration. TRES derived from long and short lifetime components were extracted from samples of different lipid species: 1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl- sn-glycero-3-phosphocholine (DOPC), d- erythro- N-palmitoyl-sphingosylphosphorylcholine (PSM), and a DOPC/PSM binary bilayer system. Neither lifetime component (short or long) corresponded with the hydration properties; the short lifetime component of DOPC (1.97 ns) exhibited a peak at 440 nm, and the long lifetime components of DPPC and PSM (7.76 and 7.77 ns, respectively) exhibited peaks at the same wavelength. This similarity arose from the competition between the collisional quenching and the hydration effects of water molecules. Herein, this phenomenon was investigated using a plot of the lifetime τ and the peak position λ (τ vs λ plot), simultaneously visualizing both effects by deconvoluting the TRES. On the basis of collisional quenching theory, the distribution of the water population per lipid (water map) was generated. According to this theory, the τ vs λ plot was applied to the water map and the calculation of the number of water molecules per lipid, which is consistent with previous reports. This approach provides novel insights for the analysis of molecular hydration states using the fluorescence of Laurdan.

  53. Solvatochromic Modeling of Laurdan for Multiple Polarity Analysis of Dihydrosphingomyelin Bilayer. International-journal Peer-reviewed

    Nozomi Watanabe, Yuka Goto, Keishi Suga, Thomas K M Nyholm, J Peter Slotte, Hiroshi Umakoshi

    Biophysical journal　116　(5)　874-883　2019/03/05

    DOI： 10.1016/j.bpj.2019.01.030 　

    More details Close

    The hydration properties of the interface between lipid bilayers and bulk water are important for determining membrane characteristics. Here, the emission properties of a solvent-sensitive fluorescence probe, 6-lauroyl-2-dimethylamino naphthalene (Laurdan), were evaluated in lipid bilayer systems composed of the sphingolipids D-erythro-N-palmitoyl-sphingosylphosphorylcholine (PSM) and D-erythro-N-palmitoyl-dihydrosphingomyelin (DHPSM). The glycerophospholipids 1-palmitoyl-2-palmitoyl-sn-glycero-3-phosphocholine and 1-oleoyl-2-oleoyl-sn-glycero-3-phosphocholine were used as controls. The fluorescence properties of Laurdan in sphingolipid bilayers indicated multiple excited states according to the results obtained from the emission spectra, fluorescence anisotropy, and the center-of-mass spectra during the decay time. Deconvolution of the Laurdan emission spectra into four components based on the solvent model enabled us to identify the varieties of hydration and the configurational states derived from intermolecular hydrogen bonding in sphingolipids. Sphingolipids showed specific, interfacial hydration properties stemming from their intra- and intermolecular hydrogen bonds. Particularly, the Laurdan in DHPSM revealed more hydrated properties compared to PSM, even though DHPSM has a higher Tm than PSM. Because DHPSM forms hydrogen bonds with water molecules (in 2NH configurational functional groups), the interfacial region of the DHPSM bilayer was expected to be in a highly polar environment. The careful analysis of Laurdan emission spectra through the four-component deconvolution in this study provides important insights for understanding the multiple polarity in the lipid membrane.

  54. Smart Preparation of Polydiacetylene Hydrogel Based on Self-Assembly of Tricosadiynoic Acid and 1-Oleoylglycerol (Monoolein) Peer-reviewed

    Keishi Suga, Yoko Otsuka, Kengo Yoshida, Hiroshi Umakoshi

    Journal of Chemical Engineering of Japan　52　(3)　311-316　2019/01

    DOI： 10.1252/jcej.18we095 　

    ISSN： 0021-9592

  55. Detection of L-Proline-Catalyzed Michael Addition Reaction in Model Biomembrane Peer-reviewed

    Masanori Hirose, Shigenori Sugisaki, Keishi Suga, Hiroshi Umakoshi

    Journal of Chemistry　2019　4926435　2019/01

    Publisher:

    DOI： 10.1155/2019/4926435 　

    ISSN： 2090-9063

    eISSN： 2090-9071

  56. Functional Hydration Behavior: Interrelation between Hydration and Molecular Properties at Lipid Membrane Interfaces Invited Peer-reviewed

    Nozomi Watanabe, Keishi Suga, Hiroshi Umakoshi

    Journal of Chemistry　2019　4867327　2019/01

    Publisher:

    DOI： 10.1155/2019/4867327 　

    ISSN： 2090-9063

    eISSN： 2090-9071

  57. Aggregation of chlorophyll a induced in self-assembled membranes composed of DMPC and DHPC Peer-reviewed

    Shogo Taguchi, Keishi Suga, Keita Hayashi, Makoto Yoshimoto, Yukihiro Okamoto, Hidemi Nakamura, Hiroshi Umakoshi

    Colloids and Surfaces B: Biointerfaces　175　403-408　2018/12

    Publisher:

    DOI： 10.1016/j.colsurfb.2018.12.008 　

    ISSN： 0927-7765

    eISSN： 1873-4367

  58. Systematic Characterization of DMPC/DHPC Self-Assemblies and Their Phase Behaviors in Aqueous Solution Peer-reviewed

    Shogo Taguchi, Keishi Suga, Keita Hayashi, Yukihiro Okamoto, Ho-Sup Jung, Hidemi Nakamura, Hiroshi Umakoshi

    Colloids and Interfaces　2　(4)　73　2018/12

    Publisher:

    DOI： 10.3390/colloids2040073 　

    eISSN： 2504-5377

  59. Direct Observation of Amyloid β Behavior at Phospholipid Membrane Constructed on Gold Nanoparticles Invited Peer-reviewed

    Keishi Suga, Ying-Chen Lai, Miftah Faried, Hiroshi Umakoshi

    International Journal of Analytical Chemistry　2018　2571808　2018/12

    Publisher:

    DOI： 10.1155/2018/2571808 　

    ISSN： 1687-8760

    eISSN： 1687-8779

  60. Effective Concentration of Ionic Liquids for Enhanced Saccharification of Cellulose Peer-reviewed

    Kazuhiko Tanimura, Yoshiko Ooe, Keishi Suga, Hiroshi Umakoshi

    ChemEngineering　2　(4)　47-8　2018/10

    Publisher:

    DOI： 10.3390/chemengineering2040047 　

    eISSN： 2305-7084

  61. Liposome Membranes Assist the L-Proline-catalyzed Aldol Reaction of Acetone and p-Nitrobenzaldehyde in Water Peer-reviewed

    Hirose Masanori, Fujiwara Shimpei, Ishigami Takaaki, Suga Keishi, Okamoto Yukihiro, Umakoshi Hiroshi

    CHEMISTRY LETTERS　47　(7)　931-934　2018/07

    DOI： 10.1246/cl.180180 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  62. Hydrolase-Like Activity Provided by Zinc(II) and Oleoyl-Histidine at Liposome Membrane Surface Peer-reviewed

    Atsushi Tauchi, Keishi Suga, Hiroshi Umakoshi

    Colloids and Interfaces　2　(2)　24　2018/06

    DOI： 10.3390/colloids2020024 　

    ISSN： 2504-5377

    eISSN： 2504-5377

  63. Characterization of DDAB/Cholesterol Vesicles and Its Comparison with Lipid/Cholesterol Vesicles Peer-reviewed

    Iwasaki Fumihiko, Suga Keishi, Okamoto Yukihiro, Umakoshi Hiroshi

    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY　18　(3)　1989-1994　2018/03

    DOI： 10.1166/jnn.2018.14252 　

    ISSN： 1533-4880

  64. Solvatochromic Property in Lipid Bilayer Interphases Evaluated from the Deconvolution of Time Resolved Emission Spectrum of Laurdan Peer-reviewed

    Watanabe Nozomi, Nyholm Thomas K. M, Slotte J. Peter, Suga Keishi, Umakoshi Hiroshi

    BIOPHYSICAL JOURNAL　114　(3)　261A　2018/02/02

    ISSN： 0006-3495

  65. Preparation and Characterization of Poly-N-isopropylacrylamide Cryogels containing Liposomes and Their Adsorption Properties of Tryptophan Peer-reviewed

    Hiramure Yui, Suga Keishi, Umakoshi Hiroshi, Matsumoto Jin, Shiomori Koichiro

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN　25　(1)　37-46　2018

    DOI： 10.15261/serdj.25.37 　

    ISSN： 1341-7215

    eISSN： 2188-4765

  66. Design of Pyrene-Fatty Acid Conjugates for Real-Time Monitoring of Drug Delivery and Controllability of Drug Release Peer-reviewed

    Keita Hayashi, Yuma Mitsuyoshi, Toshiyuki Kamei, Toshinori Shimanouchi, Keishi Suga, Yukihiro Okamoto, Hidemi Nakamura, Hiroshi Umakoshi

    ACS Omega　3　(3)　3572-3580　2018

    DOI： 10.1021/acsomega.7b02061 　

    ISSN： 2470-1343

    eISSN： 2470-1343

  67. Comparison of Physicochemical Membrane Properties of Vesicles Modified with Guanidinium Derivatives Peer-reviewed

    Nozomi Watanabe, Keishi Suga, Hiroshi Umakoshi

    JOURNAL OF PHYSICAL CHEMISTRY B　121　(39)　9213-9222　2017/10

    DOI： 10.1021/acs.jpcb.7b04007 　

    ISSN： 1520-6106

  68. Development of Time-course Oxygen Binding Analysis for Hemoglobin-based Oxygen Carriers Peer-reviewed

    Yukihiro Okamoto, Shigenori Sugisaki, Keishi Suga, Hiroshi Umakoshi

    ANALYTICAL SCIENCES　33　(8)　953-956　2017/08

    DOI： 10.2116/analsci.33.953 　

    ISSN： 0910-6340

    eISSN： 1348-2246

  69. Characterization of Liposome Membrane Containing Chlorophyll a Molecules and Its Photosensitized Functions Peer-reviewed

    Shogo Taguchi, Keishi Suga, Keita Hayashi, Makoto Yoshimoto, Yukihiro Okamoto, Hidemi Nakamura, Hiroshi Umakoshi

    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY　17　(7)　4888-4893　2017/07

    DOI： 10.1166/jnn.2017.13708 　

    ISSN： 1533-4880

    eISSN： 1533-4899

  70. Enantioselective C - C Bond Formation Enhanced by Self-Assembly of Achiral Surfactants Peer-reviewed

    Fumihiko Iwasaki, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    ACS Omega　2　(4)　1447-1453　2017/04/30

    DOI： 10.1021/acsomega.7b00034 　

    ISSN： 2470-1343

    eISSN： 2470-1343

  71. Induction of Chiral Recognition with Lipid Nanodomains Produced by Polymerization Peer-reviewed

    Yukihiro Okamoto, Yusuke Kishi, Keishi Suga, Hiroshi Umakoshi

    BIOMACROMOLECULES　18　(4)　1180-1188　2017/04

    DOI： 10.1021/acs.biomac.6b01859 　

    ISSN： 1525-7797

    eISSN： 1526-4602

  72. Preferential Adsorption of L-Histidine onto DOPC/Sphingomyelin/3 beta[N-(N ',N '-dimethylaminoethane)carbamoylicholesterol Liposomes in the Presence of Chiral Organic Acids Peer-reviewed

    Keishi Suga, Atsushi Tauchi, Takaaki Ishigami, Yukihiro Okamoto, Hiroshi Umakoshi

    LANGMUIR　33　(15)　3831-3838　2017/04

    DOI： 10.1021/acs.langmuir.6b03264 　

    ISSN： 0743-7463

  73. Multi-Level Characterization of the Membrane Properties of Resveratrol-Incorporated Liposomes Peer-reviewed

    Jin Han, Keishi Suga, Keita Hayashi, Yukihiro Okamoto, Hiroshi Umakoshi

    JOURNAL OF PHYSICAL CHEMISTRY B　121　(16)　4091-4098　2017/04

    DOI： 10.1021/acs.jpcb.7b00368 　

    ISSN： 1520-6106

  74. Adsorption Behavior of Propranolol on Negatively-Charged Liposomes and Its Influence on Membrane Fluidity and Polarity Peer-reviewed

    Takaaki Ishigami, Michael Sun Chern, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY　17　(3)　1721-1728　2017/03

    DOI： 10.1166/jnn.2017.12823 　

    ISSN： 1533-4880

    eISSN： 1533-4899

  75. Fluorescent Probe Study of AOT Vesicle Membranes and Their Alteration,upon Addition of Aniline or the Aniline Dimer p-Aminodiphenylamine (PADPA) Peer-reviewed

    Fumihiko Iwasaki, Sandra Luginbuhl, Keishi Suga, Peter Walde, Hiroshi Umakoshi

    LANGMUIR　33　(8)　1984-1994　2017/02

    DOI： 10.1021/acs.langmuir.6b04480 　

    ISSN： 0743-7463

  76. Liposomes Can Achieve Enantioselective C-C Bond Formation of an α-Amino Acid Derivative in Aqueous Media Peer-reviewed

    Fumihiko Iwasaki, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    ACS Omega　2　(1)　91-97　2017/01/31

    DOI： 10.1021/acsomega.6b00479 　

    ISSN： 2470-1343

    eISSN： 2470-1343

  77. Adsorption properties of tryptophan with cryogel containing liposomes

    Y. Hiramure, K. Suga, H. Umakoshi, K. Shiomori

    Proceedings of The 29th International Symposium on Chemical Engineering　PF16　2016/12/03

  78. Liposomes modified with cardiolipin can act as a platform to regulate the potential flux of NADP+-dependent isocitrate dehydrogenase Peer-reviewed

    Keishi Suga, Akari Hamasaki, Junpei Chinzaka, Hiroshi Umakoshi

    Metabolic Engineering Communications　3　8-14　2016/12/01

    Publisher: Elsevier

    DOI： 10.1016/j.meteno.2015.11.002 　

    ISSN： 2214-0301

    eISSN： 2214-0301

  79. Characterization of Aqueous Oleic Acid/Oleate Dispersions by Fluorescent Probes and Raman Spectroscopy Peer-reviewed

    Keishi Suga, Dai Kondo, Yoko Otsuka, Yukihiro Okamoto, Hiroshi Umakoshi

    LANGMUIR　32　(30)　7606-7612　2016/08

    DOI： 10.1021/acs.langmuir.6b02257 　

    ISSN： 0743-7463

  80. In Situ Cell Surface Modification for Surface-enhanced Raman Analysis of Cell Membrane Peer-reviewed

    Yukihiro Okamoto, Takumi Ikeda, Keishi Suga, Hiroshi Umakoshi

    CHEMISTRY LETTERS　45　(6)　622-624　2016/06

    DOI： 10.1246/cl.160137 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  81. Roles of Sterol Derivatives in Regulating the Properties of Phospholipid Bilayer Systems Peer-reviewed

    Tham Thi Bui, Keishi Suga, Hiroshi Umakoshi

    LANGMUIR　32　(24)　6176-6184　2016/06

    DOI： 10.1021/acs.langmuir.5b04343 　

    ISSN： 0743-7463

  82. Effect of Boundary Edge in DOPC/DPPC/Cholesterol Liposomes on Acceleration of L-Histidine Preferential Adsorption Peer-reviewed

    Takaaki Ishigami, Atsushi Tauchi, Keishi Suga, Hiroshi Umakoshi

    LANGMUIR　32　(24)　6011-6019　2016/06

    DOI： 10.1021/acs.langmuir.5b04626 　

    ISSN： 0743-7463

  83. Effect of Stearylguanidinium-Modified POPC Vesicles on the Melting Behavior of tRNA Molecules Peer-reviewed

    Keishi Suga, Nozomi Watanabe, Hiroshi Umakoshi

    JOURNAL OF PHYSICAL CHEMISTRY B　120　(25)　5662-5669　2016/06

    DOI： 10.1021/acs.jpcb.6b04198 　

    ISSN： 1520-6106

  84. Quantitative Monitoring of Microphase Separation Behaviors in Cationic Liposomes Using HHC, DPH, and Laurdan: Estimation of the Local Electrostatic Potentials in Microdomains Peer-reviewed

    Keishi Suga, Kei Akizaki, Hiroshi Umakoshi

    LANGMUIR　32　(15)　3630-3636　2016/04

    DOI： 10.1021/acs.langmuir.5b04682 　

    ISSN： 0743-7463

  85. Chiral Selective Adsorption of Ibuprofen on a Liposome Membrane Peer-reviewed

    Yukihiro Okamoto, Yusuke Kishi, Takaaki Ishigami, Keishi Suga, Hiroshi Umakoshi

    JOURNAL OF PHYSICAL CHEMISTRY B　120　(10)　2790-2795　2016/03

    DOI： 10.1021/acs.jpcb.6b00840 　

    ISSN： 1520-6106

  86. Liposome membrane can induce self-cleavage of RNA that models the core fragments of hammerhead ribozyme Peer-reviewed

    Keishi Suga, Seishiro Tanaka, Hiroshi Umakoshi

    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS　45　(1)　55-62　2016/01

    DOI： 10.1007/s00249-015-1076-z 　

    ISSN： 0175-7571

    eISSN： 1432-1017

  87. Liposome Membrane as a Platform for the L-Pro-Catalyzed Michael Addition of trans-beta-Nitrostyrene and Acetone Peer-reviewed

    Masanori Hirose, Takaaki Ishigami, Keishi Suga, Hiroshi Umakoshi

    LANGMUIR　31　(47)　12968-12974　2015/12

    DOI： 10.1021/acs.langmuir.5b03439 　

    ISSN： 0743-7463

  88. Homochiral oligomerization of L-histidine in the presence of liposome membranes Peer-reviewed

    Takaaki Ishigami, Yoshinori Kaneko, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    COLLOID AND POLYMER SCIENCE　293　(12)　3649-3653　2015/12

    DOI： 10.1007/s00396-015-3764-8 　

    ISSN： 0303-402X

    eISSN： 1435-1536

  89. High performance optical resolution with liposome immobilized hydrogel Peer-reviewed

    Takaaki Ishigami, Kazuma Sugita, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    COLLOIDS AND SURFACES B-BIOINTERFACES　136　256-261　2015/12

    DOI： 10.1016/j.colsurfb.2015.09.009 　

    ISSN： 0927-7765

    eISSN： 1873-4367

  90. Preparation of Smart Vesicle Modified with C-Glycoside Ketohydrazon for the Development of Novel DDS Peer-reviewed

    Keita Hayashi, Madoka Kiriishi, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

    Journal of Drug Delivery　2015/10

    DOI： 10.1155/2015/481670 　

  91. Chiral Recognition of L-Amino Acids on Liposomes Prepared with L-Phospholipid Peer-reviewed

    Takaaki Ishigami, Keishi Suga, Hiroshi Umakoshi

    ACS APPLIED MATERIALS & INTERFACES　7　(38)　21065-21072　2015/09

    DOI： 10.1021/acsami.5b07198 　

    ISSN： 1944-8244

  92. Characterization of the physicochemical properties of phospholipid vesicles prepared in CO2/water systems at high pressure Peer-reviewed

    Hidemi Nakamura, Shogo Taguchi, Keishi Suga, Keita Hayashi, Ho-Sup Jung, Hiroshi Umakoshi

    BIOINTERPHASES　10　(3)　2015/09

    DOI： 10.1116/1.4928722 　

    ISSN： 1934-8630

    eISSN： 1559-4106

  93. Pseudo-Interphase of Liposome Promotes 1,3-Dipolar Cycloaddition Reaction of Benzonitrile Oxide and N-Ethylmaleimide in Aqueous Solution Peer-reviewed

    Fumihiko Iwasaki, Keishi Suga, Hiroshi Umakoshi

    JOURNAL OF PHYSICAL CHEMISTRY B　119　(30)　9772-9779　2015/07

    DOI： 10.1021/acs.jpcb.5b03762 　

    ISSN： 1520-6106

  94. Microfluidic platforms with monolithically integrated hierarchical apertures for the facile and rapid formation of cargo-carrying vesicles Peer-reviewed

    Hyesung Cho, Junsoo Kim, Keishi Suga, Takaaki Ishigami, Hyunchul Park, Jung Won Bang, Soonmin Seo, Mansoo Choi, Pahn-Shick Chang, Hiroshi Umakoshi, Sup Jung, Kahp-Yang Suh

    LAB ON A CHIP　15　(2)　373-377　2015

    DOI： 10.1039/c4lc01096e 　

    ISSN： 1473-0197

    eISSN： 1473-0189

  95. Partitioning of Hydrophobic Molecules to Liposome Membranes Can Induce Variations in their Micro-Polarity and Micro-Viscosity Peer-reviewed

    Fumihiko Iwasaki, Keishi Suga, Dai Kondo, Hiroshi Umakoshi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN　22　79-85　2015

    DOI： 10.15261/serdj.22.79 　

    ISSN： 1341-7215

  96. Investigation of Fatty Acid Ketohydrazone Modified Liposome's Properties as a Drug Carrier Peer-reviewed

    Hayashi Keita, Kiriishi Madoka, Suga Keishi, Okamoto Yukihiro, Umakoshi Hiroshi

    JOURNAL OF DRUG DELIVERY　2015

    DOI： 10.1155/2015/481670 　

    ISSN： 2090-3014

  97. Systematical Characterization of Phase Behaviors and Membrane Properties of Fatty Acid/Didecyldimethylammonium Bromide Vesicles Peer-reviewed

    Keishi Suga, Tomoya Yokoi, Dai Kondo, Keita Hayashi, Seiichi Morita, Yukihiro Okamoto, Toshinori Shimanouchi, Hiroshi Umakoshi

    LANGMUIR　30　(43)　12721-12728　2014/11

    DOI： 10.1021/la503331r 　

    ISSN： 0743-7463

  98. Heterogeneous Cationic Liposomes Modified with 3 beta-{N-[(N ',N '-Dimethylamino)ethyl]carbamoyl}cholesterol Can Induce Partial Conformational Changes in Messenger RNA and Regulate Translation in an Escherichia coli Cell-Free Translation System Peer-reviewed

    Keishi Suga, Tomoyuki Tanabe, Hiroshi Umakoshi

    LANGMUIR　29　(6)　1899-1907　2013/02

    DOI： 10.1021/la3050576 　

    ISSN： 0743-7463

  99. Use Liposome as a Designable Platform for Molecular Recognition similar to from "Statistical Separation" to "Recognitive Separation" Peer-reviewed

    Hiroshi Umakoshi, Keishi Suga

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN　20　1-13　2013

    ISSN： 1341-7215

  100. Hydrophobic Properties of tRNA with Varied Conformations Evaluated by an Aqueous Two-Phase System Peer-reviewed

    Keishi Suga, Hibiki Tomita, Seishiro Tanaka, Hiroshi Umakoshi

    INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES　8　(8)　1188-1196　2012

    DOI： 10.7150/ijbs.5059 　

    ISSN： 1449-2288

  101. Conformational change of single-stranded RNAs induced by liposome binding Peer-reviewed

    Keishi Suga, Tomoyuki Tanabe, Hibiki Tomita, Toshinori Shimanouchi, Hiroshi Umakoshi

    NUCLEIC ACIDS RESEARCH　39　(20)　8891-8900　2011/11

    DOI： 10.1093/nar/gkr568 　

    ISSN： 0305-1048

  102. Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System Peer-reviewed

    Hiroshi Umakoshi, Tomoyuki Tanabe, Keishi Suga, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

    INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES　7　(3)　253-260　2011

    DOI： 10.7150/ijbs.7.253 　

    ISSN： 1449-2288

  103. Cationic liposome can interfere mRNA translation in an E. coli cell-free translation system Peer-reviewed

    Huong Thi Bui, Hiroshi Umakoshi, Keishi Suga, Tomoyuki Tanabe, Kien Xuan Ngo, Toshinori Shimanouchi, Ryoichi Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL　52　(1)　38-43　2010/10

    DOI： 10.1016/j.bej.2010.07.001 　

    ISSN： 1369-703X

    eISSN： 1873-295X

  104. Liposomes destabilize tRNA during heat stress Peer-reviewed

    Keishi Suga, Hiroshi Umakoshi, Hibiki Tomita, Tomoyuki Tanabe, Toshinori Shimanouchi, Ryoichi Kuboi

    BIOTECHNOLOGY JOURNAL　5　(5)　526-529　2010/05

    DOI： 10.1002/biot.200900289 　

    ISSN： 1860-6768

  105. Charged liposome affects the translation and folding steps of in vitro expression of green fluorescent protein Peer-reviewed

    Hiroshi Umakoshi, Keishi Suga, Huong Thi Bui, Masato Nishida, Toshinori Shimanouchi, Ryoichi Kuboi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING　108　(5)　450-454　2009/11

    DOI： 10.1016/j.jbiosc.2009.05.012 　

    ISSN： 1389-1723

  106. Negatively charged liposome as a potent inhibitor of post-translation during in vitro synthesis of green fluorescent protein Peer-reviewed

    Huong Thi Bui, Hiroshi Umakoshi, Keishi Suga, Masato Nishida, Toshinori Shimanouchi, Ryoichi Kuboi

    BIOCHEMICAL ENGINEERING JOURNAL　46　(2)　154-160　2009/10

    DOI： 10.1016/j.bej.2009.05.002 　

    ISSN： 1369-703X

  107. Cationic Liposome Inhibits Gene Expression in an E. coli Cell-Free Translation System Peer-reviewed

    Bui, H. T, Umakoshi, H, Suga, K, Tanabe, T, Shimanouchi, T, Kuboi, R

    Membrane　34　(3)　146-151　2009/03

    Publisher:

  108. Characterization of Green Fluorescent Protein Using Aqueous Two-Phase Systems Peer-reviewed

    Hiroshi Umakoshi, Masato Nishida, Keishi Suga, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

    SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN　16　145-150　2009

    ISSN： 1341-7215

  109. Morphological Estimation of DMPC/DHPC Self-Assemblies in Diluted Condition: Based on Physicochemical Membrane Properties

    Keishi Suga

    DOI： 10.20944/preprints201809.0242.v1 　

Show all ︎Show first 5

Misc. 10

  1. Chiral Recognition / Conversion on Liposome

    馬越大, 菅恵嗣

    膜　44　(2)　69‐75(J‐STAGE)　2019

    DOI： 10.5360/membrane.44.69 　

    ISSN： 0385-1036

  2. Characterization of Molecular Behaviors on Phospholipid Membrane Surface based on Membrane Surface-Enhanced Raman Spectroscopy Method

    菅恵嗣, 馬越大

    表面と真空　62　(4)　194‐197(J‐STAGE)　2019

    DOI： 10.1380/vss.62.194 　

    ISSN： 2433-5835

  3. クロロフィルa会合体の形成に対するディスク状脂質膜の効果および会合体の光捕集

    田口翔悟, 菅恵嗣, 林啓太, 岡本行広, 中村秀美, 馬越大

    化学工学会年会研究発表講演要旨集(CD-ROM)　83rd　2018

  4. 構造依存的特性に基づいた薬剤カプセル~薬剤の封入と細胞への応用~

    村田祐輔, 盤井秀香, 林啓太, 菅恵嗣, 岡本行広, 中村秀美, 馬越大

    日本膜学会年会講演要旨集　40th　2018

  5. 脂質分子集合体界面におけるクロロフィルaの凝集挙動の評価ならびに光エネルギー変換への応用

    田口翔悟, 菅恵嗣, 林啓太, 岡本行広, 中村秀美, 馬越大

    化学工学会年会研究発表講演要旨集(CD-ROM)　82nd　2017

  6. ディスク状脂質膜の相挙動に基づくクロロフィルaの分子配向制御とその応用

    田口翔悟, 菅恵嗣, 林啓太, 岡本行広, 中村秀美, 馬越大

    化学工学会秋季大会研究発表講演要旨集(CD-ROM)　49th　2017

  7. 吸着分離材のメゾスケールデザイン ソフトマター界面の秩序構造を活用するキラル医薬品/RNAの分離

    菅恵嗣, 馬越大

    分離技術　46　(6)　357‐362-362　2016/11/30

    Publisher: 分離技術会

    ISSN： 1343-7860

  8. 超臨界二酸化炭素を用いたリポソームの調製とその膜特性の解析

    田口翔悟, 菅恵嗣, 岡本行広, 馬越大, 林啓太, 中村秀美

    化学工学会秋季大会研究発表講演要旨集(CD-ROM)　46th　2014

  9. 非溶媒超臨界逆相蒸発法により調製したリポソームの特性解析

    田口翔悟, 菅恵嗣, 馬越大, 林啓太, 中村秀美

    化学工学会秋季大会研究発表講演要旨集(CD-ROM)　45th　2013

  10. A “Membranome”―Based Approach toward “Bio‐Inspired Membrane”

    馬越大, 島内寿徳, 菅恵嗣

    膜　37　(6)　264-269　2012/11/01

    Publisher: THE MEMBRANE SOCIETY OF JAPAN

    DOI： 10.5360/membrane.37.264 　

    ISSN： 0385-1036

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    It is important to establish a methodology to design and develop a "Bio-Inspired Membrane", which can be defined as an "Artificial Membrane" inspired by structures and functions observed in "Biomembrane". Several approaches relating to this research area are reviewed based on a hierarchical view about the structures and characteristics of the above membranes. A "Membranome" research utilizing a gel membrane immobilizing liposome membrane at high concentration is herewith introduced as one of several approaches to create the "Bio-Inspired Membrane". Some possible data required for the "Bio-Inspired Membrane" are finally discussed, focusing on the physicochemical property of self-assembly included in a total system.

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Presentations 12

  1. 脂質膜界面評価を基盤とするバイオインターフェイスの設計に関する研究 Invited

    菅恵嗣

    日本膜学会第46年会　2024/06/12

  2. 脂質の自己集合から発想する新奇な機能性粒子の開発 Invited

    菅恵嗣

    第19回日本セラミックス協会MFD研究会　2024/03/27

  3. Design of Soft Matter Interface at Nanoscale: toward Developments of Bioactive Nanomaterials

    Keishi Suga

    2022/09/18

  4. リポソーム膜におけるミクロ～メゾスケール相分離挙動の評価

    菅 恵嗣

    生物工学Webシンポジウム2020　2020/09/02

  5. 物質を認識して分けるための膜材料開発

    菅 恵嗣

    分離技術会年会2019　2019/05/25

  6. 菅 恵嗣 Invited

    2019/03/14

  7. 膜表面増強ラマン法(MSERS)を活用するリン脂質膜における分子挙動解析手法の開発

    菅 恵嗣

    2018年日本表面真空学会学術講演会　2018/11/21

  8. Development of fatty acid-based self-assembly platform: ~ from nano capsule to hydrogel~ Invited

    Keishi Suga

    2018 KosFoST International Symposium and Annual Meeting　2018/06/27

  9. Development and Characterization of Fatty Acid Assembly ~ From Nano Capsule to Hierarchical Gel ~

    Keishi Suga, Hiroshi Umakoshi

    2017 Agricultural Biotechnology Symposium　2017/11/09

  10. ソフトマター界面の秩序構造を活用するキラル医薬品/RNAの分離

    菅 恵嗣, 馬越 大

    第22回関西地区分離技術会講演会　2016/12/12

  11. 自己組織化膜を基盤とする分子認識プロセスの設計 Invited

    菅 恵嗣

    材料化学システム工学討論会2015　2015/09/01

  12. Membranomeを基盤とするBio-Inspired化学工学 ～リポソーム膜を活用する生体分子の認識・機能制御を目指して～

    馬越 大, 菅 恵嗣

    最先端光計測とライフサイエンスの近未来 -バイオ・ラマン2017-　2013/08/08

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Research Projects 12

  1. ナノギャップエンジニアリングの基盤構築

    中村 暢伴, 菅 恵嗣

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 大阪大学

    2024/04/01 - 2027/03/31

  2. 形状依存ビルディングブロック集積による細胞内ナノ粒子の高効率機能発現

    長尾 大輔, 林 久美子, 新井 敏, 菅 恵嗣, 山内 紀子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(A)

    Institution: 東北大学

    2023/04 - 2027/03

  3. Continuous synthesis of cubosomal self-assembly for macromolecular drug deliverys

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2022/04/01 - 2026/03/31

  4. Continuous synthesis of cubosomal self-assembly for macromolecular drug deliverys

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2022/04 - 2026/03

  5. Establish a Strategy to Utilize "Membrane Platform" for Expanding the World of Bio-Inspired Chemical Engineering

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Osaka University

    2021/04 - 2026/03

  6. 分子選択性を有する表面増強ラマン散乱用金属ナノ粒子の開発

    Nakamura Nobutomo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Osaka University

    2020/07 - 2022/03

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    In this study, we developed metallic nanoparticles for surface-enhanced Raman scattering with molecular selectivity using the originally developed piezoelectric resonance method. We fabricated nanoparticles with extremely narrow gaps on substrate, and evaluated detectability of molecules with changing the gap size. As a result, we confirmed that it is possible to evaluate the molecular size using the developed metallic nanoparticles.

  7. Development of Separation Process Using a Hierarchical Structure of Cubosomal Self-Assembly Gel

    Suga Keishi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Category: Grant-in-Aid for Early-Career Scientists

    2019/04 - 2022/03

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    Cubosomal self-assembly was successfully prepared by fatty acid (e.g., oleic acid) and monoolein. Raman spectroscopy analysis revealed that lipids in cubosome were tightly packed, which could be one of essential properties of hierarchical cubosome assembly. In the gel preparation process, a high-speed stirring (Re > 2000) was necessary, wherein long-time shear stress application could promote the fluid dynamic aggregation of cubosome particles. The obtained cubosomal gel encapsulated hydrophobic drugs and metal nanoparticles. It is expected that cubosomal self-assembly (particle, gel) can be applied for drug delivery system and separation device.

  8. Microscopic and Macroscopic Characterization of Self-Assembled Materials for Concerted Molecular Recognition

    Suga Keishi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Osaka University

    2016/04 - 2019/03

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    In the present research project, a novel membrane-based material has been developed. The ordered phase of self-assembled lipid membrane showed significant molecular recognition ability of L-amino acid. By using multiple fluorescent probes, various kinds of self-assembly systems were systematically characterized. In oleic acid/monoolein systems, a gel-phase like ordered membrane properties was observed (in microscopic view): these properties were common to cubic phase (cubosome) assemblies. These systems are gel states (in macroscopic view), thus they can be utilized as hierarchical membrane materials. To increase molecular recognition for protein (ex, amyloid beta) and nucleic acid (ex, tRNA), the membranes modified with phosphoserine and guanidinium ligand were effective, respectively. In their interactions between target, the membrane was dehydrated together with concerted conformational changes of target molecules.

  9. Membrane-on-Membrane ~Creation of Hierarchical Self-Organizing Materials~

    Umakoshi Hiroshi, Ishigami Takaaki, Iwasaki Fumihiko, Hirose Masanori, Taguchi Shogo, Watanabe Nozomi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Osaka University

    2014/04 - 2019/03

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    In this project, a novel membranous devise (material) was developed. Membrane-on-Membrane (MoM) was hierarchically composed by self-assembled membranes. Liposome was selected as core unit of MoM, because it showed unique functions such as recognition of L-amino acid, promoting organic reaction in water, and molecular orientation of chlorophyll. In addition, the methodologies, multiple-fluorescent probe analyses, membrane surface-enhanced Raman spectroscopy, dielectric dispersion (relaxation) spectroscopy, were developed to characterize the physicochemical properties of membranes (in micro- and mesoscale). As an example of our achievements, the “ordered” membrane property plays a crucial role to improve selectivity in chiral recognition of amino acid. When the liposome possessing ordered membrane property was applied to MoM device (for example, liposome-immobilized polyacrylamide hydrogel system), the chiral recognition ability could be maintained.

  10. 抗酸化物質から成るCubosomeの開発およびミクロ・メソ・マクロ特性の評価

    Offer Organization: 公益財団法人コーセーコスメトロジー研究財団

    System: 第27回コスメトロジー研究助成

    Institution: 大阪大学

    2016/04 - 2018/03

  11. ベシクル膜による“アンサンブル型”分子認識・変換反応

    Offer Organization: 日本学術振興会

    System: 研究活動スタート支援

    Category: 研究活動スタート支援

    Institution: 大阪大学

    2013/10 - 2015/03

  12. "生体膜干渉"とその応用に関する研究

    菅 恵嗣

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 特別研究員奨励費

    Institution: 大阪大学

    2011 - 2012

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    本研究では,モデル生体膜であるリボソーム膜を「場」とする分子の振舞いに着目し,脂質膜上における生体高分子十本鎖RAA,ポリペプチド,他)の認識機構ならびに高次構造・機能制御について取り組んだ.特に,リボソーム膜は一本鎖RNA分子と選択的に相互作用する事を明らかにし,モデル生体膜が直接的にRNA機能を制御する"生体膜干渉"という概念を見出した(cf.RNA干渉). まず,生体高分子を認識するための「場」のデザインとして,種々のリン脂質およびコレステロールより構成されるリボソーム膜を調製し,その物理化学的な膜特性解析を行った.各種分光解析(蛍光分光,赤外分光,ラマン分光)により,脂質膜の相状態およびミクロ相分離挙動について検討を行い,粒径100nmのリボソーム膜上に10-30Åのナノドメインが形成されることを明らかにした.以上の結果は投稿論文2報に掲載済みである(Langmuir,29(15),4830-4838(2013);Solv.Extr.Res.Dev.Jpn.,20,1-13(2013).また,これら知見について各学会にて発表を行い,専門家らとの意見交換を行った(国内学会計3件). 次に,表層デザインリポソーム膜を利用して生体高分子の認識機構について検討を行った結果,ナノドメイン性リボソーム膜は複数の相互作用(静電相互作用,疎水性相互作用,水素結合,表層環境の変化(脱水和),表面構造の合致)により一本鎖RNA分子を高選択的に膜表層へ誘導する事を明らかにした.さらにバルク環境とは異なる膜表層環境において,anA分子の高次構造が制御され,RNA機能(mRNA翻訳,Hammerhead Ribozyme自己切断)が制御可能である事を明らかにした.1RNA分子の表面特性および高次構造の解析手法を確立(円二色性分光,ラマン分光)し,脂質膜が生体高分子認識のための「場」として活用できる事を明らかにした.以上の結果は投稿論文に掲載済みである(Int'l.J.Bio1.Sci.,8(8),1188-1196(2012);Langmuir,29(6),1899-1907(2013);膜,37(6),264-269(2012).また,これら知見について各学会にて発表を行い,専門家らとの意見交換を行った(国内学会計5件).

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