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Megumi Nakamura

Section

Graduate School of Dentistry

Job title

Senior Assistant Professor

Degree

博士（歯学）（東北大学）

researchmap

https://researchmap.jp/tissuebiology

J-GLOBAL ID

200901051121642257

Research History 7

2021/12 - Present

Tohoku University Graduate School of Dentistry　Craniofacial Development and Tissue Biology　Senior Assistant Professor
2017/04 - Present

宮城高等歯科衛生士学院　非常勤講師
2013/04 - 2023/03

仙台保健福祉専門学校　歯科衛生士科　非常勤講師
2019/04 - 2021/11

Tohoku University　Graduate School of Dentistry
2007/04 - 2019/03

Tohoku University Graduate School of Dentistry　Division of Craniofacial Development and Regeneration　Assistant professor
2006/04 - 2007/03

Tohoku University　Graduate School of Dentistry
2005/04 - 2006/03

東北大学歯学部付属病院　口腔外科研修医

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Education 2

Tohoku University　Graduate School, Division of Dental Research

2001/04 - 2005/03
Tohoku University　Faculty of Dentistry　歯学科

1995/04 - 2001/03

Committee Memberships 3

歯科基礎医学会　代議員

2024 - Present
日本解剖学会　認定解剖組織技術者資格審査委員会委員

2023 - Present
日本学術振興会　科学研究費委員会専門委員

2021/11 - 2022/10

Professional Memberships 4

JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Japanese Association of Anatomists(2008/06-)
歯科基礎医学会(2004/07-)
日本口腔外科学会(2002/12-2009/04) (2017/4-)

Research Interests 7

Resorption of Meckel's cartilage
Expression of MMPs and ADAMTSs during development and aging
Effects of aging on oral tissues
Developmnet of bone and teeth
Roles of osteoclasts in the process of bone development and healing
Tooth germ transplantation
Bone healing process

Research Areas 2

Life sciences / Surgical dentistry /
Life sciences / Oral medicine /

Awards 2

Outstanding award (poster presentation)

2024/10　2024 TU-HKU-FJMU International Symposium on Oral Health Science)　Mineral concentration and distribution in enamel mineralization analyzed by SEM/EDS
優秀口演発表賞ノミネート(10演題)

2017/10　第62回日本口腔外科学会学術大会　歯胚移植における凍結保存の実験的応用

Papers 24

Mineral Content and Extracellular Matrix Protein Expression in Mouse Growth Plates During Epiphyseal Fusion: An Observational Study Peer-reviewed

Xinhang Yu, Megumi Nakamura, Miyuki Mayanagi, Itaru Mizoguchi, Yasuyuki Sasano

Calcified Tissue International　2025/06/06

DOI： 10.1007/s00223-025-01391-9 　
Immunohistochemical analysis of MMP-9 and MMP-13 expression in ligature-induced periodontitis mouse model Peer-reviewed

菅野千春, 中村恵, 大方広志, 山本寿則, 笹野泰之

東北大学歯学雑誌　42　(2)　2023/12

ISSN： 0287-3915
Remodeling during standardized parietal bone defect repair in mice Peer-reviewed

猪狩洋平, 中村恵, 服部佳功, 笹野泰之

東北大学歯学雑誌　42　(2)　2023/12

ISSN： 0287-3915
Age-Related Gene and Protein Expression in Mouse Mandibular Condyle Analyzed by Cap Analysis of Gene Expression and Immunohistochemistry. International-journal Peer-reviewed

Mu-Chen Yang, Megumi Nakamura, Yoko Kageyama, Yohei Igari, Yasuyuki Sasano

Gerontology　69　(11)　1295-1306　2023/09

DOI： 10.1159/000533921 　

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INTRODUCTION: Aging, an inevitable physiological process, leads to morphological and histological degenerative changes in the mandibular condylar cartilage (MCC); however, the molecular mechanism has not yet been elucidated, and little information is available on age-related factors. Therefore, this study was designed to identify age-related factors by investigating the age-related differentially expressed genes (DEGs) and localization of their translated protein expression in the mandibular condyle. METHODS: Mandibular condyles were collected from 10- and 50-week-old mice. Total RNA was extracted from the samples and then analyzed using cap analysis of gene expression (CAGE) to identify age-related DEGs. Gene ontology (GO) enrichment analysis was performed to determine which biological processes were most affected by aging in terms of gene expression using Metascape. The mandibular condyle samples were processed for histology to investigate morphological changes caused by aging and for immunohistochemistry to localize the protein expression encoded by age-related genes identified with CAGE. Semi-quantitative immunohistochemistry was performed to assess age-related extracellular matrix (ECM) protein levels in the MCC. The histological sections were also used for Alcian blue histochemistry to detect glycosaminoglycans (GAGs). RESULTS: GO enrichment analysis revealed that the genes related to "extracellular matrix organization," including Acan, Col1a1, Col1a2, Col2a1, Mmp3, Mmp9, and Mmp13, were most differentially expressed in the aged mandibular condyle. Among these seven genes, Mmp3 was upregulated, and the others were downregulated with aging. Histological examination showed the age-related morphological and histological changes in the MCC. Immunohistochemical investigation showed the localization of matrix metalloproteinases (MMPs)-3, -9, and -13 and their substrate proteins, aggrecan, type I collagen, and type II collagen, in the mandibular condyle at 10 and 50 weeks, indicating different localizations between the young and the aged. In the aged MCC, semi-quantitative immunohistochemistry showed a significant decrease in the aggrecan protein level, and Alcian blue histochemistry showed a decrease in GAGs. CONCLUSION: MMP-3, MMP-9, and MMP-13 contribute to the remodeling of the ECM of the MCC and subchondral bone during aging by degrading ECM proteins at specific times and sites under the regulation of their production and secretion.
Visualization of the localization of phospholipids in developing rat teeth by matrix-assisted laser desorption/ionization imaging mass spectrometry Peer-reviewed

Yasuyuki SASANO, Alu KONNO, Megumi NAKAMURA, Akiko HENMI, Miyuki MAYANAGI, Mu-Chen YANG, Ikuko YAO

Biomedical Research　44　(4)　173-179　2023/08/03
Publisher: Biomedical Research Press
DOI： 10.2220/biomedres.44.173 　

ISSN： 0388-6107

eISSN： 1880-313X
Immunohistochemical characterization of the mandibular condyle for type I and II collagen, aggrecan, MMP-9, and MMP-13 in MMP-2-deficient mice Peer-reviewed

Mu-Chen YANG, Megumi NAKAMURA, Miyuki MAYANAGI, Yasuyuki SASANO

Biomedical Research　44　(2)　65-72　2023/04/02
Publisher: Biomedical Research Press
DOI： 10.2220/biomedres.44.65 　

ISSN： 0388-6107

eISSN： 1880-313X
The visualization of the mineral and protein distribution in the same histological sections of rat calcified growth plate cartilage Peer-reviewed

Miyuki Mayanagi, Megumi Nakamura, Akiko Henmi, Yasuyuki Sasano

Journal of Oral Biosciences　2022/09
Publisher: Elsevier BV
DOI： 10.1016/j.job.2022.08.005 　

ISSN： 1349-0079
Expression of matrix metalloproteinase‐3 and ‐10 is up‐regulated in the periodontal tissues of aged mice International-journal Peer-reviewed

Yoko Kageyama, Megumi Nakamura, Yohei Igari, Satoshi Yamaguchi, Akiko Oguchi, Yasuhiro Murakawa, Yoshinori Hattori, Yasuyuki Sasano

Journal of Periodontal Research　57　(4)　733-741　2022/08
Publisher: Wiley
DOI： 10.1111/jre.12996 　

ISSN： 0022-3484

eISSN： 1600-0765

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OBJECTIVE: The present study was designed to investigate the whole transcriptome of periodontal tissues of both young and aged mice to identify the characteristic up-regulation of protease genes with aging and to localize their translated protein products in the periodontal tissues. BACKGROUND: The metzincin protease superfamily is composed of matrix metalloproteinases (MMPs), a disintegrin and metalloproteinases, and a disintegrin and metalloproteinases with thrombospondin motifs. Up-regulation of these extracellular matrix-degrading proteases has been implicated in senescence of tissues and organs, including the skin. However, few studies have investigated the expression profiles of these proteases and potential involvement in aging of periodontal tissues. METHODS: Periodontal tissues with the surrounding mandibular bones were collected from 50- and 10-week-old mice. Total RNA was extracted from the periodontal tissue and analyzed by cap analysis of gene expression (CAGE) to identify differentially expressed genes encoding the metzincin proteases. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the CAGE results, and the phenotypic expression of proteases involved in aging was localized via immunohistochemical analysis. RESULTS: The CAGE results showed that the expression levels of MMP-3, -10, and -12 were up-regulated at 50 weeks. Subsequent qRT-PCR analysis showed that the gene expression levels of MMP-3 and -10 were significantly increased with age. MMP-10 immunoreactivity was localized exclusively in the cementum and alveolar bone adjacent to the periodontal ligament and was stronger and broader in aged mice than young mice. MMP-3 immunoreactivity was localized in the periodontal ligaments at both 10 and 50 weeks. CONCLUSION: In the present study, we demonstrated that the expression of MMP-3 and -10 increased with aging and identified their characteristic localizations in aged periodontal tissues.
Calcification and resorption of mouse Meckel’s cartilage analyzed by von Kossa and tartrate-resistant acid phosphatase histochemistry and scanning electron microscopy/energy-dispersive X-ray spectrometry Peer-reviewed

Megumi Nakamura, Mu-Chen Yang, Keijyu Ashida, Miyuki Mayanagi, Yasuyuki Sasano

Anatomical Science International　97　(2)　213-220　2022/03
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s12565-021-00643-6 　

ISSN： 1447-6959

eISSN： 1447-073X

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Meckel's cartilage is essential for the normal development of the mandible. The fate of the intermediate portion of Meckel's cartilage is unique as most of it disappears soon after birth except for the part that forms the sphenomandibular ligament. The mechanism of the disappearance of Meckel's cartilage is unknown; therefore, this study was designed to investigate the process of Meckel's cartilage degradation, focusing on cartilage matrix calcification and the appearance of chondroclasts. Developing mouse mandibles at embryonic days 15, 16, 17, and 18, and postnatal day 2 were processed for whole-mount staining with alcian blue and alizarin red. The mandibles on embryonic days 15, 16, 17, and 18 were fixed and embedded in paraffin. Adjacent sections were processed for von Kossa and tartrate-resistant acid phosphatase (TRAP) histochemistry and scanning electron microscopy/energy-dispersive X-ray spectrometry (SEM/EDS). Calcification and the element concentrations of calcium, phosphorus, and carbon were examined with von Kossa histochemistry and SEM/EDS. The involvement of chondroclasts was investigated using TRAP histochemistry. The results demonstrated that the intermediate portion of Meckel's cartilage is resorbed by chondroclasts after chondrocyte hypertrophy and cartilage matrix calcification and that the mineral concentration of calcified Meckel's cartilage is comparable to that of the surrounding bone. This study contributes to the understanding of the mechanism of Meckel's cartilage resorption and provides useful insights into the development of the mandible.
Three-dimensional visualization of osteoclasts in embryonic mouse mandibles using SEM array tomography International-journal Peer-reviewed

Liao Caiyu, Megumi Nakamura, Miyuki Mayanagi, Atsuko Kayaba, Yasuyuki Sasano

Journal of Oral Biosciences　63　(4)　401-407　2021/12
Publisher: Elsevier BV
DOI： 10.1016/j.job.2021.10.003 　

ISSN： 1349-0079

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OBJECTIVE: Three-dimensional (3-D) images of osteoclasts in vivo have been elusive, due to their large size and intricate morphology. The present study was designed to reconstruct the 3-D morphology of whole osteoclasts in developing mouse mandibles using scanning electron microscopy (SEM) array tomography. METHODS: Mandibles of 16 days post coitum mouse embryos were fixed and embedded in epoxy resin after decalcification. Epoxy blocks were trimmed, and serial sections of 1 μm in thickness were cut with an ultramicrotome and mounted on glass microscope slides. Consecutive images of every fourth or fifth serial section were obtained by SEM after electron staining and platinum coating. Three dimensional reconstruction of osteoclasts was performed using these consecutive images. RESULTS: Multinucleated osteoclasts were observed to cluster around developing bone in the embryonic mouse mandible. The outlines of osteoclasts and their sealing zones were identified in the serial sections. The reconstructed 3-D image revealed whole osteoclast morphology with the sealing zone. Osteoclasts were adherent to bone with the anchoring structure between the osteoclast and the bone. CONCLUSIONS: SEM array tomography with our modification revealed 3-D imagery of a whole osteoclast and its sealing zone in vivo for the first time. This methodology could provide useful information on in vivo structures and dynamics of large cells, such as osteoclasts.
Application of cryopreservation to tooth germ transplantation for root development and tooth eruption Peer-reviewed

Xinghan Li, Megumi Nakamura, Weidong Tian, Yasuyuki Sasano

Scientific Reports　11　(1)　2021/05
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41598-021-88975-1 　

eISSN： 2045-2322

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<title>Abstract</title>We cryopreserved mouse tooth germs with widely open cervical margins of the enamel organ to overcome difficulties in cryoprotectant permeation and tested their efficacy by transplanting them into recipient mice. The upper right first molar germs of 8-day-old donor mice were extracted and categorized into the following four groups according to cryopreservation time: no cryopreservation, 1 week, 1 month, and 3 months. The donor tooth germs were transplanted into the upper right first molar germ sockets of the 8-day-old recipient mice. The upper left first molars of the recipient mice were used as controls. The outcome of the transplantation was assessed at 1, 2, and 3 weeks after transplantation. Stereomicroscopic evaluation revealed that most of the transplanted teeth erupted by 3 weeks after transplantation. Micro-computed tomography analysis revealed root elongation in the transplanted groups as well as in the controls. There was no significant difference between the cryopreserved and non-cryopreserved transplanted teeth, but the roots of the cryopreserved teeth were significantly shorter than those of the control teeth. Histological examination revealed root and periodontal ligament formations in all the transplanted groups. These results suggest that the transplantation of cryopreserved tooth germs facilitates subsequent root elongation and tooth eruption.
Expression of tartrate-resistant acid phosphatase and cathepsin K during osteoclast differentiation in developing mouse mandibles Peer-reviewed

Megumi NAKAMURA, Naoki AOYAMA, Satoshi YAMAGUCHI, Yasuyuki SASANO

Biomedical Research　42　(1)　13-21　2021/02/10
Publisher: Biomedical Research Press
DOI： 10.2220/biomedres.42.13 　

ISSN： 0388-6107

eISSN： 1880-313X
分析走査電子顕微鏡を用いた硬組織の石灰化の進行と成熟過程の検討 Peer-reviewed

笹野泰之, 中村恵, 逸見晶子, 大方広志, 眞栁みゆき

顕微鏡　54　(3)　2019/11
Degradation of extracellular matrices propagates calcification during development and healing in bones and teeth. International-journal Peer-reviewed

Yasuyuki Sasano, Megumi Nakamura, Akiko Henmi, Hiroshi Okata, Osamu Suzuki, Atsuko Kayaba, Miyuki Mayanagi

Journal of oral biosciences　61　(3)　149-156　2019/09

DOI： 10.1016/j.job.2019.07.004 　

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BACKGROUND: Bone, dentin, and enamel are tissues formed through calcification, a process involving deposition of calcium phosphate minerals on extracellular organic matrices. Calcification, the underlying mechanism of which is unknown, is initiated with mineral deposition followed by advancing of the deposit and subsequent maturation of the mineral crystal. HIGHLIGHT: We have reviewed the current knowledge of how calcification proceeds during bone development, bone healing, and enamel and dentin development, based on reported studies. Previous studies reported by us and by other authors have suggested that degradation of some extracellular matrix (ECM) proteins is involved in calcification during bone and dentin development and bone healing in a manner similar to that previously reported for enamel development. CONCLUSION: The ECM proteins may inhibit mineral deposition and calcification, similar to the role of amelogenin during enamel development. The candidates for the amelogenin equivalents in bone and dentin have not been identified. Further studies are required to elucidate the regulatory mechanisms of bone and dentin calcification in light of specific ECM proteins that prevent calcification and enzymes that degrade these ECM proteins.
加齢マウス脛骨における成長板軟骨の組織構造と皮質骨の石灰化に関する検討 Peer-reviewed

猪狩洋平, 影山曜子, 萱場敦子, 眞栁みゆき, 中村恵, 服部佳功, 笹野泰之

東北大学歯学雑誌　36/37　32-36　2018/12
Experimental Comparison of the Performance of Cutting Bone and Soft Tissue between Piezosurgery and Conventional Rotary Instruments. International-journal Peer-reviewed

Otake Y, Nakamura M, Henmi A, Takahashi T, Sasano Y

Scientific reports　8　(1)　17154-17154　2018/11

DOI： 10.1038/s41598-018-35295-6 　

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Piezosurgery is an innovative technique widely used for osteotomies in the field of oral and maxillofacial surgery. The surgical technique has been clinically supposed to cut mineralized bone selectively with reducing the risk of damage to adjacent soft tissues. However, none of the previous literature has reported any evidence of scientific experiments to examine performance of the piezoelectric device, i.e. the time required for cutting bone and the effect on soft tissues under the standardized conditions. This study was designed to test the hypothesis that cutting time of the piezoelectric device is longer than that of rotary instruments while the cut surface of bone is smoother and soft tissues are less damaged with piezosurgery under the standardized experimental system. We measured the time for cutting bone and soft tissues of rats with the piezoelectric device and rotary instruments. Damage to soft tissues was examined histologically, and the cut surface of bone was investigated using scanning electron microscopy. Our study demonstrated experimentally that piezosurgery provides a smooth cut bony surface with no damage to soft tissues and takes longer time to cut bone than conventional drillings. We propose that piezosurgery is beneficial for medical safety and usability.
Calcification during bone healing in a standardised rat calvarial defect assessed by micro-CT and SEM-EDX Peer-reviewed

H. Okata, M. Nakamura, A. Henmi, S. Yamaguchi, Y. Mikami, H. Shimauchi, Y. Sasano

ORAL DISEASES　21　(1)　74-82　2015/01

DOI： 10.1111/odi.12212 　

ISSN： 1354-523X

eISSN： 1601-0825
Remodeling of extracellular matrices initiates and advances calcification during development and healing of bones and teeth Peer-reviewed

Yasuyuki Sasano, Megumi Nakamura, Hiroshi Okata, Akiko Henmi, Yasuto Mikami

Journal of Oral Biosciences　54　(1)　25-29　2012
Publisher: Japanese Association for Oral Biology
DOI： 10.1016/j.job.2011.06.001 　

ISSN： 1349-0079
Involvement of sensory neurons in bone defect repair in rats Peer-reviewed

Akiko Henmi, Megumi Nakamura, Seishi Echigo, Yasuyuki Sasano

Journal of Electron Microscopy　60　(6)　393-400　2011/12

DOI： 10.1093/jmicro/dfr075 　

ISSN： 0022-0744
Root and periodontal tissue development after allogenic tooth transplantation between rat littermates Peer-reviewed

K. Andou, M. Nakamura, Y. Ina, K. Sasaki, Y. Sasano

ORAL DISEASES　17　(4)　379-386　2011/05

DOI： 10.1111/j.1601-0825.2010.01761.x 　

ISSN： 1354-523X

eISSN： 1601-0825
Bone formation in rat calvaria ceases within a limited period regardless of completion of defect repair Peer-reviewed

T. Honma, T. Itagaki, M. Nakamura, S. Kamakura, I. Takahashi, S. Echigo, Y. Sasano

ORAL DISEASES　14　(5)　457-464　2008/07

DOI： 10.1111/j.1601-0825.2007.01401.x 　

ISSN： 1354-523X
Analysis of appositional bone formation using a novel rat experimental model Peer-reviewed

D. Nakajima, S. Kamakura, M. Nakamura, O. Suzuki, S. Echigo, Y. Sasano

ORAL DISEASES　14　(4)　308-313　2008/05

DOI： 10.1111/j.1601-0825.2007.01377.x 　

ISSN： 1354-523X
Expression of versican and ADAMTS1, 4, and 5 during bone development in the rat mandible and hind limb Peer-reviewed

M Nakamura, S Sone, Takahashi, I, Mizoguchi, I, S Echigo, Y Sasano

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY　53　(12)　1553-1562　2005/12

DOI： 10.1369/jhc.5A6669.2005 　

ISSN： 0022-1554
Expression of versican and ADAMTS during rat tooth eruption Peer-reviewed

S Sone, M Nakamura, Y Maruya, Takahashi, I, Mizoguchi, I, H Mayanagi, Y Sasano

JOURNAL OF MOLECULAR HISTOLOGY　36　(4)　281-288　2005/05

DOI： 10.1007/s10735-005-5534-2 　

ISSN： 1567-2379

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Misc. 32

Enamel biomineralization and proteolysis of amelogenin during mouse firstmolar development

韓宇, 中村恵, 笹野泰之

Journal of Oral Biosciences Supplement　2024　[O2-06]　2024/11
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
New insights into growth plate closure: effects of aging on growth plate morphology, mineral content, and extracellular matrix in mice

余心航, 中村恵, 笹野泰之

Journal of Oral Biosciences Supplement　2024　[O3-05]　2024/11
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
マウス実験的歯周炎におけるMMP9とMMP13の発現に関する検討

菅野千春, 中村恵, 大方広志, 山本寿則, 笹野泰之

日本歯周病学会会誌　65　(秋季特別)　128-128　2023/10
Publisher: (NPO)日本歯周病学会
ISSN： 0385-0110

eISSN： 1880-408X
マウス実験的歯周炎モデルを用いた加齢と炎症が及ぼす歯槽骨吸収への影響

中村恵, Yang Mu-Chen, 山口哲史, 大方広志, 笹野泰之

日本歯周病学会会誌　65　(秋季特別)　129-129　2023/10
Publisher: (NPO)日本歯周病学会
ISSN： 0385-0110

eISSN： 1880-408X
マウス実験的歯周炎における歯槽骨吸収に対する加齢と炎症の影響

中村恵, Yang Mu-Chen, 笹野泰之

Journal of Oral Biosciences Supplement　2023　[P2-12]　2023/09
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
骨形成の場を整える吸収系細胞の検討下顎の発生過程において石灰化組織吸収に寄与する細胞(Cells responsible for resorption of calcified tissues during mandibular development)

中村恵, Yang Mu-Chen, 笹野泰之

Journal of Oral Biosciences Supplement　2022　64-64　2022/09
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
高齢マウスの顎関節における網羅的遺伝子解析(Whole transcriptome profiling and immunohistochemical analysis in the TMJ condyle of aged mice)

楊牧蓁, 中村恵, 笹野泰之

Journal of Oral Biosciences Supplement　2021　121-121　2021/10
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
分子組織細胞化学の最前線と未来への展望硬組織石灰化進行過程の分析走査電子顕微鏡による元素イメージング解析

笹野泰之, 中村恵, 逸見晶子, 大方広志, 鈴木治, 真柳みゆき

日本組織細胞化学会総会・学術集会講演プログラム・予稿集　61回　31-31　2020/12
Publisher: 日本組織細胞化学会
オーラル・バイオロジーの老化研究への新たな挑戦マウス歯周組織における老化関連細胞外マトリックス分解酵素の探索トランスクリプトーム解析を用いたアプローチ

中村恵, 笹野泰之

Journal of Oral Biosciences Supplement　2020　141-141　2020/09
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
網羅的遺伝子解析を用いた高齢マウスの下顎における細胞外マトリックス分解酵素の発現

影山曜子, 中村恵, 猪狩洋平, 山口哲史, 服部佳功, 笹野泰之, 小口綾貴子, 村川泰裕

Journal of Oral Biosciences Supplement　2019　230-230　2019/10
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
組織細胞化学的アプローチで解く硬組織の新たな構造と機能凍結保存歯胚の移植における歯の萠出と歯根形成

中村恵, 笹野泰之

日本組織細胞化学会総会・学術集会講演プログラム・予稿集　60回　64-64　2019/09
Publisher: 日本組織細胞化学会
組織化学イメージングで探る硬組織の細胞機能発生・修復に伴う硬組織石灰化の元素イメージング

笹野泰之, 逸見晶子, 大方広志, 中村恵, 鈴木治

日本組織細胞化学会総会・学術集会講演プログラム・予稿集　59回　43-43　2018/09
Publisher: 日本組織細胞化学会
マウス下顎骨発生過程では破骨細胞は石灰化に先行して出現する

青山直樹, 中村恵, 笹野泰之

Journal of Oral Biosciences Supplement　2017　395-395　2017/09
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
凍結保存歯胚を移植すると、歯根の伸長と歯の萠出が起こる

中村恵, 笹野泰之, Li Xinghan

Journal of Oral Biosciences Supplement　2017　425-425　2017/09
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
骨と軟組織に対するピエゾサージェリーの切削能に関する実験的検討

大竹義雄, 中村恵, 逸見晶子, 高橋哲, 笹野泰之

Journal of Oral Biosciences Supplement　2017　315-315　2017/09
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
骨欠損修復における骨基質の石灰化に関する検討

大方広志, 中村恵, 逸見晶子, 島内英俊, 笹野泰之

Journal of Oral Biosciences Supplement　2012　119-119　2012/09
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
Effects of Capsaicin Treatment on Nociception and Structure of Trigeminal Nerve Fibers Peer-reviewed

Kato A, Nakamura M, Echigo S, Sasano Y

Interface Oral Health Science 2011　140-142　2011/03
Root Elongation and Periodontal Tissue Formation in Tooth Germs Allogeneically Transplanted Between Rat Littermates Peer-reviewed

Ando K, Nakamura M, Ina Y, Sasaki K, Sasano Y

Interface Oral Health Science 2011　147-149　2011/03
マイクロCTを利用した同腹他個体ラット間移植における歯根伸長の検討

安藤浩二, 中村恵, 笹野泰之

解剖学雑誌　85　(2)　55-55　2010/06
Publisher: (一社)日本解剖学会
ISSN： 0022-7722
カプサイシンを利用した骨修復における感覚神経の役割の検討

加藤晶子, 中村恵, 越後成志, 笹野泰之

解剖学雑誌　85　(Suppl.)　111-111　2010/03
Publisher: (一社)日本解剖学会
ISSN： 0022-7722
同腹他個体ラット間の根未形成歯移植における歯根伸長と歯周組織形成に関する検討

安藤浩二, 中村恵, 伊奈慶典, 佐々木啓一, 笹野泰之

Journal of Oral Biosciences　51　(Suppl.)　92-92　2009/08
Publisher: (一社)歯科基礎医学会
ISSN： 1349-0079

eISSN： 1880-3865
カプサイシンの全身投与が知覚および三叉神経線維の形態に及ぼす影響

加藤晶子, 中村恵, 越後成志, 笹野泰之

Journal of Oral Biosciences　51　(Suppl.)　99-99　2009/08
Publisher: (一社)歯科基礎医学会
ISSN： 1349-0079

eISSN： 1880-3865
ラット頭蓋骨のcriticalとnon-criticalな欠損の修復過程に関するX線学的定量解析と遺伝子組織学検討

本間崇裕, 板垣智子, 中村恵, 鎌倉慎治, 越後成志, 笹野泰之

日本口腔外科学会雑誌　54　(1)　28-28　2008/01
Publisher: (公社)日本口腔外科学会
ISSN： 0021-5163
Quantitative-radiographic and molecular-histological analysis of bone repair in critical and non-critical size rat calvarial bone defects Peer-reviewed

Honma T, Itagaki T, Nakamura M, Kamakura S, Takahashi I, Echigo S, Sasano Y

Interface oral Health Science 2007　371-372　2007/09
Expression of bone matrix proteins and matrix metalloproteinases during repair of rat calvarial bone defects Peer-reviewed

Tomoko Itagaki, Takahiro Honma, Megumi Nakamura, Ichiro Takahashi, Seishi Echigo, Yasuyuki Sasano

Interface Oral Health Science 2007　335-340　2007/09
骨欠損修復過程における骨基質タンパクとMMPの発現に関する定量的解析と局在検索

板垣智子, 本間崇裕, 中村恵, 高橋一郎, 越後成志, 笹野泰之

Journal of Oral Biosciences　49　(Suppl.)　114-114　2007/08
Publisher: (一社)歯科基礎医学会
ISSN： 1349-0079

eISSN： 1880-3865
骨欠損修復過程における骨芽細胞分化と骨基質タンパク産生に関する細胞分子生物学的検討

板垣智子, 本間崇裕, 中村恵, 高橋一郎, 越後成志, 笹野泰之

Journal of Oral Biosciences　48　(Suppl.)　127-127　2006/09
Publisher: (一社)歯科基礎医学会
ISSN： 1349-0079

eISSN： 1880-3865
骨欠損の残存の有無にかかわらず、骨修復は一定期間内で停止する

本間崇裕, 板垣智子, 中村恵, 鎌倉慎治, 越後成志, 笹野泰之

Journal of Oral Biosciences　48　(Suppl.)　112-112　2006/09
Publisher: (一社)歯科基礎医学会
ISSN： 1349-0079

eISSN： 1880-3865
Versican and ADAMTSs are involved in bone development Peer-reviewed

M. Nakamura, I. Takahashi, S. Echigo, Y. Sasano

International Congress Series　1284　336-337　2005/09
Publisher: Elsevier BV
DOI： 10.1016/j.ics.2005.06.015 　

ISSN： 0531-5131
下顎と下肢の骨発生過程におけるversicanとADAMTS1,4,5の発現

中村恵, 笹野泰之

解剖学雑誌　80　(1)　12-12　2005/03
Publisher: (一社)日本解剖学会
ISSN： 0022-7722
ラット臼歯の萌出過程におけるversican及びADAMTSの発現

曽根信哉, 中村恵, 丸谷由里子, 高橋一郎, 溝口到, 真柳秀昭, 笹野泰之

Journal of oral biosciences　46　(5)　380-380　2004/09/01
Publisher: 歯科基礎医学会
ISSN： 1349-0079
下顎と下肢の骨発生・成長過程におけるADAMTS1、4、5及びversican、aggrecanの発現

中村恵, 曽根信哉, 高橋一郎, 溝口到, 越後成志, 笹野泰之

Journal of oral biosciences　46　(5)　403-403　2004/09/01
Publisher: 歯科基礎医学会
ISSN： 1349-0079

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Presentations 54

The epiphyseal fusion region of the mouse growth plate is composed of calcified cartilage instead of bone

Xinhang Yu, Megumi Nakamura, Miyuki Mayanagi, Itaru Mizoguchi, Yasuyuki Sasano

APPW2025　2025/03/18
Temporal study of enamel biomineralization and amelogenin degradation by MMP-20 in developing teeth

Yu Han, Megumi Nakamura, Miyuki Mayanagi, Yasuyuki Sasano

APPW2025　2025/03/18
Enamel Biomineralization and Proteolysis of Amelogenin during Mouse First Molar Development

Yu HAN, Megumi NAKAMURA, Miyuki MAYANAGI, Yasuyuki SASANO

2024/11/03
New insights into growth plate closure: effects of aging on growth plate morphology, mineral content, and extracellular matrix in mice

Xinhang Yu, Megumi Nakamura, Yasuyuki Sasano

2024/11/04
Mineral concentration and distribution in enamel mineralization analyzed by scanning electron microscopy/energy dispersive X-ray spectrometry

Yu Han, Megumi Nakamura, Miyuki Mayanagi, Jiang Chen, Yasuyuki Sasano

2024/10/19
A search for age-related matrix metalloproteinases in mouse mandible using whole transcriptome analysis Invited

Megumi Nakamura

【国際】Tohoku Bhakta Dental Symposium (TBDS)　2024/06/25
マウス実験的歯周炎モデルを用いた加齢と炎症が及ぼす歯槽骨吸収への影響

中村恵, Mu-Chen Yang, 山口哲史, 大方広志, 笹野泰之

第66回秋季日本歯周病学会学術大会　2023/10/13
マウス実験的歯周炎におけるMMP9とMMP13の発現に関する検討

菅野千春, 中村恵, 大方広志, 山本寿則, 笹野泰之

第66回秋季日本歯周病学会学術大会　2023/10/13
Immunohistochemical localization of MMP-9, MMP-13, and extracellular matrix proteins in the mandibular condyle of MMP-2-deficient mice

Mu Chen Yang, Megumi Nakamura, Yasuyuki Sasano

2023/09/16
マウス実験的歯周炎における歯槽骨吸収に対する加齢と炎症の影響

中村恵, Yang Mu-Chen, 笹野泰之

第65回歯科基礎医学会学術大会　2023/09/17
下顎骨発生における破骨細胞・破軟骨細胞の役割と意義 Invited

中村恵, Mu-Chen Yang, 笹野泰之

第128回日本解剖学会総会・全国学術集会シンポジウム「骨発生・成長における破骨細胞と周囲の細胞群の連携機構」　2023/03/18
Whole transcriptome analysis with CAGE revealed that MMPs-3, -9, and -13 were differentially expressed in the mandibular condyle of aged mice

Yang Mu-Chen, Megumi Nakamura, Yasuyuki Sasano

2023/03/20
Cells responsible for resorption of calcified tissues during mandibular developmen Invited

Nakamura M, Yang MC, Sasano Y

2022/09/18
Whole transcriptome profiling and immunohistochemical analysis in the TMJ condyle of aged mice

2021/10/09
CAGE解析を用いたマウス歯周組織における老化関連細胞外マトリックス分解酵素の探索

中村恵, 笹野泰之

日本解剖学会第67回東北・北海道連合支部学術集会　2021/09/04
硬組織石灰化進行過程の分析走査電子顕微鏡による元素イメージング解析 Invited

笹野泰之, 中村恵, 逸見晶子, 大方広志, 鈴木治, 真柳みゆき

第61回日本組織細胞化学会総会・学術集会シンポジウム「分子組織細胞化学の最前線と未来への展望」　2020/12/17
マウス歯周組織における老化関連細胞外マトリックス分解酵素の探索―トランスクリプトーム解析を用いたアプローチ― Invited

中村恵, 笹野泰之

第62回歯科基礎医学会学術大会「オーラル・バイオロジーの老化研究への新たな挑戦」　2020/10/03
マウス下顎のトランスクリプトーム解析による老化関連細胞外マトリックス分解酵素の同定

影山曜子, 中村恵, 猪狩洋平, 山口哲史, 服部佳功, 笹野泰之

第125回日本解剖学会総会・全国学術集会　2020/03/26
網羅的遺伝子解析を用いた高齢マウスの下顎における細胞外マトリックス分解酵素の発現

影山曜子, 中村恵, 猪狩洋平, 山口哲史, 服部佳功, 笹野泰之

第61回歯科基礎医学会学術大会　2019/10/12
凍結保存歯胚の移植における歯の萌出と歯根形成 Invited

中村恵, 笹野泰之

第60回日本組織細胞化学会総会・学術集会ワークショップ「組織細胞化学的アプローチで解く硬組織の新たな構造と機能」　2019/09/19
凍結保存歯胚移植に伴う硬組織形成の形態学的解析 Invited

中村恵, 笹野泰之

第１２4回日本解剖学会総会・全国学術集会シンポジウム「硬組織の細胞分化と再生におけるニューパラダイム：形態学的アプローチ」　2019/03/27
発生・修復に伴う硬組織石灰化の元素イメージング Invited

笹野泰之, 逸見晶子, 大方広志, 中村恵, 鈴木治

第59回日本組織細胞化学会総会・学術集会ワークショップ「組織化学イメージングで探る硬組織の細胞機能」　2018/09/29
Morphogensis of Osteoclasts during Mandibular Bone Development

Naoki Aoyama, Megumi Nakamura, Faculty Advisor

2018/08/24
Three-dimensional visualization of embryonic bone development with serial-semithin-section SEM International-presentation

Liao Caiyu, Atsuko Kayaba, Miyuki Mayanagi, Megumi Nakamura, Yasuyuki Sasano

2018/08/05
骨と軟組織の切削におけるピエゾサージェリーと回転切削器具の実験的比較検討

大竹義雄, 中村恵, 逸見晶子, 笹野泰之, 高橋哲

第62回日本口腔外科学会・学術大会　2017/10/20
歯胚移植における凍結保存の実験的応用

Megumi Nakamura

第62回日本口腔外科学会総会・学術大会　2017/10/20

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優秀口演賞ノミネート演題
凍結保存歯胚を移植すると、歯根が伸長し歯が萌出する

中村恵, 笹野泰之

第59回歯科基礎医学会学術大会　2017/09/16
マウス下顎骨発生過程では破骨細胞は石灰化に先行して出現する

青山直樹, 中村恵, 笹野泰之

第59回歯科基礎医学会学術大会　2017/09/16
骨と軟組織に対するピエゾサージェリーの切削能に関する実験的検討

大竹義雄, 中村恵, 逸見晶子, 高橋哲, 笹野泰之

第59回歯科基礎医学会学術大会　2017/09/16
Transplantation of cryopreserved mouse tooth germ

Li Xinghan, Megumi Nakamura, Yasuyuki Sasano

第122回日本解剖学会総会・全国学術集会　2017/03/28
骨の発生・修復に伴う石灰化成熟 Invited

笹野泰之, 逸見晶子, 大方広志, 中村恵, 鈴木治

第121回日本解剖学会総会・全国学術集会、シンポジウム「石灰化研究の新展開」　2016/03/28
Calcification in healing calvarial bone assessed by micro-CT and SEM-EDX International-presentation

Okata H, Nakamura M, Henmi A, Yamaguchi S, Miakami Y, Sasano Y

【国際】第5回インターフェイス口腔健康科学国際シンポジウム　2014/01/20
Analysis of calcification in bone healing with a standardized rat calvarial defect model International-presentation

国際】Okata H, Nakamura M, Henmi A, Yamaguchi S, Mikami Y, Shimauchi H, Sasano Y

International Symposium Anatomical Science for adavance in health and clinical therapy (ASahct) 2013　2013/08/27
硬組織の発生・修復における細胞外基質リモデリングと石灰化 Invited

笹野泰之, 逸見晶子, 大方広志, 三上靖人, 中村恵

第118回日本解剖学会総会・全国学術集会研究集会・懇話会（歯の発生の会）　2013/03/27
骨密度と構成元素の解析を利用した修復骨基質の石灰化の検討

大方広志, 中村恵, 逸見晶子, 島内英俊, 笹野泰之

第58回東北・北海道連合支部学術集会　2012/09/22
骨欠損修復における骨基質の石灰化に関する検討

大方広志, 中村恵, 逸見晶子, 島内英俊, 笹野泰之

第５４回歯科基礎医学会学術大会　2012/09/15
実験的骨欠損における修復骨形成過程の石灰化進行に関する検討

大方広志, 中村恵, 逸見晶子, 島内英俊, 笹野泰之

第117回日本解剖学会総会・全国学術大会　2012/03/26
Effects of capsaicin treatment on nociception and structure of trigeminal nerve fibers in adult rats International-presentation

Akiko Kato, Megumi Nakamura, Seishi Echigo, Yasuyuki Sasano

【国際】第４回インターフェイス口腔健康科学国際シンポジウム　2011/03/07
Root Elongation and Periodontal Tissue Formation in Tooth Germs Allogeneically Transplanted Between Rat Littermates International-presentation

Koji Ando, Megumi Nakamura, Yoshinori Ina, Keiichi Sasaki, Yasuyuki Sasano

【国際】第４回インターフェイス口腔健康科学国際シンポジウム　2011/03/07
カプサイシンを利用した骨修復における感覚神経の役割の検討

加藤晶子, 中村恵, 越後成志, 笹野泰之

第115回日本解剖学会総会・全国学術集会　2010/03/28
マイクロＣＴを利用した同腹他個体ラット間移植における歯根伸長の検討

安藤浩二, 中村恵, 笹野泰之

第55回東北・北海道連合支部学術集会　2009/09/26
カプサイシンの全身投与が知覚および三叉神経線維の形態に及ぼす影響

加藤晶子, 中村恵, 越後成志, 笹野泰之

第51回歯科基礎医学会学術大会　2009/09/09
同腹他個体ラット間の根未形成歯移植における歯根伸長と歯周組織形成に関する検討

安藤浩二, 中村恵, 伊奈慶典, 佐々木啓一, 笹野泰之

第51回歯科基礎医学会学術大会　2009/09/09
骨欠損修復過程における骨基質タンパクとMMPの発現に関する定量的解析と局在検索

板垣智子, 本間崇裕, 中村恵, 高橋一郎, 越後成志, 笹野泰之

第49回歯科基礎医学会総会　2007/08/30
Osteoblasts and osteocytes cease bone formation and ECM remodeling ithin a limited period regardless of comletion of bone healing in the defect International-presentation

Sasano Yasuyuki, Itagaki Tomoko, Honma Takahiro, Nakamura Megumi, Takahashi Ichiro

【国際】Gordon Research Conferences　2007/07/15
Quantitative-radiographic and molecular-histological analysis of bone repair in critical and non-critical size rat calvarial bone defects International-presentation

Honma T, Itagaki T, Nakamura M, Kamakura S, Takahashi I, Echigo S, Sasano Y

【国際】第2回インターフェイス口腔健康科学国際シンポジウム　2007/02
Expression of bone matrix proteins and matrix metalloproteinases during repair of rat calvarial bone defects International-presentation

Itagaki T, Honma T, Nakamura M, Takahashi I, Echigo S, Sasano Y

【国際】第2回インターフェイス口腔健康科学国際シンポジウム　2007/02
骨欠損の残存にかかわらず、骨修復は一定期間で停止する

本間崇裕, 板垣智子, 中村恵, 鎌倉慎治, 越後成志, 笹野泰之

第48回歯科基礎医学会　2006/09/21
骨欠損修復過程における骨芽細胞分化と骨基質タンパク産生に関する細胞分子生物学的検討

板垣智子, 本間崇裕, 中村恵, 高橋一郎, 越後成志, 笹野泰之

第48回歯科基礎医学会　2006/09/21
Versican and ADAMTSs are involved in bone development International-presentation

Nakamura, Megumi, Takahashi, Ichiro, Echigo, Seishi, Sasano, Yasuyuki

【国際】インターフェイス口腔健康科学国際シンポジウム　2005/02/02
下顎と下肢の骨発生過程におけるversicanとADAMTS1,4,5の発現

中村恵, 笹野泰之

日本解剖学会第50回東北・北海道連合地方会　2004/10/16
ラット臼歯の萌出過程におけるversican及びADMATSの発現

曽根信哉, 中村恵, 丸谷由里子, 高橋一郎, 溝口到, 真柳秀昭, 笹野泰之

第46回歯科基礎医学会学術大会　2004/09/23
下顎と下肢の骨発生・成長過程におけるADMATS1,4,5及びversican,aggrecanの発現

中村恵, 曽根信哉, 高橋一郎, 溝口到, 越後成志, 笹野泰之

第46回歯科基礎医学会学術大会　2004/09/23
Identification of age-related extracellular proteases in periodontal tissues of aged mice using whole transcriptome analysis with CAGE International-presentation Invited

Megumi Nakamura

【国際】21st Yonsei Dental International Symposium, Innovation and challenge of Geriatric dentistry　2022/12/16

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Industrial Property Rights 1

三次元画像処理装置、三次元画像処理方法及びプログラム

山口哲史, 中村恵

Property Type: Patent

Research Projects 12

プラズマFIB-SEMとCAGEを用いたDAP12/FcRγ欠損マウスの歯の萌出に関する研究

中村恵

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2025/04/01 - 2028/03/31
GFPマウス歯胚移植における歯根膜再生メカニズムの解明

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2021/04 - 2025/03
Experimental investigation of bone repair using a mouse model lacking osteoclast differentiation: are osteoclasts necessary for bone repair?

Nakamura Megumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2018/04/01 - 2022/03/31

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The incidence of c-fos deficient (-/-) mice was examined by genotyping with PCR using DNA extracted from embryos obtained from mating between c-fos heterozygous (+/-) mice. The results showed that heterozygous (+/-) mice accounted for more than half of the total number of mice (61.2%), while the incidence of c-fos deficient (-/-) mice was low at 14.3%. Micro-CT analysis was performed at 4, 8, and 12 weeks after creation of a standardized defect in the parietal bone of wild-type mice to examine the process of bone defect repair. The results showed that the amount of repaired bone increased over time, and that repaired bone was not only formed from the margins of the defect, but also sporadically formed within the defect. Furthermore, our histological analysis with hematoxylin and eosin staining showed consistent findings with the results of micro-CT analysis.
Identification of ECM proteins that inhibit calcification in bone and dentin using matrix-assisted laser desorption/ionization imaging mass spectrometry

SASANO YASUYUKI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2015/04 - 2019/03

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The study was designed to investigate matrix molecules characteristic for bone and dentin using matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS). We examined methods to apply IMS for the calcified tissues and adopted 4% paraformaldehyde fixation - freeze with or without decalcification in 10% EDTA since information about the tissue preparation was not available. Developing rat teeth at 3 days were processed for IMS analysis. The data was acquired in the positive ion mode with 75μm and 10μm pitches and a mass range setting of 0 to 1000. Characteristic localization of signals in the tooth germs was shown at m/z 706 and 732. The signals were localized in the area of dental papillae and enamel epithelia in the tooth germ. The data base search indicated that the corresponding molecules are phosphatidylcholines. Sudan Black B staining showed comparable localization of lipids in the tooth germ.
網羅的遺伝子解析を用いた破骨細胞分化抑制マウスにおける歯の萌出機序の解明 Competitive

中村恵

Offer Organization: 日本学術振興会科学研究費助成事業

System: 基盤研究(C)

2015/04 - 2018/03
Analysis of bone healing process with the in vivo targeting system of NFkB decoy

Nakamura Megumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

Category: Grant-in-Aid for Young Scientists (B)

Institution: Tohoku University

2012/04 - 2016/03

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This study was designed to investigate bone healing process of the standardized calvarial defect in rats with downregulation of inflammation-related molecules by nuclear factor-kappa B (NFkB) decoy to elucidate the role of inflammation in bone repair. So far, we succeeded to establish the in vivo targeting system using type I collagen sponge as a carrier. In addition, we showed the mRNA expression of proinflammatory cytokines in developing bone of which bone formation process is comparable to that of healing bone.
Investigation of the role of MMP in bone healing with mineral and proteome analyses

SASANO YASUYUKI, NAKAMURA Megumi, TAKAHASHI Nobuhiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2011/04 - 2015/03

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The present study was designed to investigate the process of calcification during bone healing in a standardized rat calvarial bone defect model. A standard defect with diameter 3.8 mm was made on the parietal bone of 12-week-old male Wistar rats under anesthesia. The healing bone was analyzed with micro-computed tomography and energy dispersive X-ray spectroscopy in weeks 1,2,4 and 8. The bone mineral density and the Ca/P ratio increased, whereas the C/Ca and C/P ratios decreased during bone healing. The present study demonstrated that the mineral components increase in density and mature in quality while organic components decrease. Our following study revealed the comparable calcification process during development of rat calvarial bone development.
石灰化と骨成熟におけるMMPの機能の探索 Competitive

笹野泰之, 高藤泰子, 中村恵, 三上靖人

Offer Organization: 独立行政法人科学技術振興機構

System: 研究シーズ探索プログラム

2011/04 - 2012/03
Study of involvement of sensory neurons in bone defect repair in rats with capsaicin-induced sensory denervation

NAKAMURA Megumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

Category: Grant-in-Aid for Young Scientists (B)

Institution: Tohoku University

2008 - 2011

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We investigated bone repair in sensory-denervated rats. Nine-week-old male Wistar rats received subcutaneous injections of capsaicin to denervate sensory neurons. Rats treated with the same amount of vehicle served as controls. A standardized bone defect was created on the parietal bone. We measured the amount of repaired bone with quantitative radiographic analysis and the mRNA expressions of osteocalcin and cathepsin K with real-time PCR. The results suggested that capsaicin-induced sensory denervation affects the bone defect repair and remodeling.
A role of MMP family molecules in remodeling ECM and advancing calcification

SASANO Yasuyuki, NAKAMURA Megumi, AKITA Hirotoshi, SUZUKI Osamu

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2008 - 2010

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An organ culture system to model the physiological calcification process was designed using rat embryonic calvaria as a device for analyzing its mechanism. Standardized calvarial explants were dissected from rat embryos aged day 18 and cultured for 1, 3, and 5 days. The Ca content was examined with atomic absorption spectrophotometry and distribution of Ca and the concentration of Ca, P and C were examined with von Kossa stain and/or SEM-EDX. The result indicates that the organ culture system using E18 calvaria is useful for modeling of the physiological calcification process in vitro.
顎骨の発生・成長におけるIL-1及びTNF一αのADAMTS発現誘導機構の解析

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (Start-up)

Category: Grant-in-Aid for Young Scientists (Start-up)

Institution: Tohoku University

2006 - 2007
移植歯胚への遣伝子およびsiRNA導入を利用した歯根形成誘導機構の解明

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Exploratory Research

Category: Grant-in-Aid for Exploratory Research

Institution: Tohoku University

2005 - 2007

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Teaching Experience 10

Life science C　Tohoku University
発生学（講義）　東北大学
Oral histology（Lectures）　Tohoku University
Oral embryology（Lectures）　Tohoku University
Histology（Lectures）　Tohoku University
Basic research course　Tohoku University
Oral embryology（Labs）　Tohoku University
Oral histology（Labs）　Tohoku University
Histology（Labs）　Tohoku University
再生・創建医歯学　Tohoku University

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Media Coverage 3

歯の凍結保存歯髄損傷せず移植に成功

日本歯科新聞

2021/05/25

Type: Newspaper, magazine
歯胚の凍結保存移植で歯が萌出東北大

WHITE CROSS 歯科医師向け情報サイト

2021/05/21

Type: Internet
東北大、歯根形成前の歯胚を用いた凍結保存移植で移植後に歯根の伸長と歯根膜の形成が起こり歯が萌出することを明らかに

日本経済新聞（電子版）

2021/05/10

Type: Newspaper, magazine