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Kaoru Hiramoto

Section

Frontier Research Institute for Interdisciplinary Sciences

Job title

Assistant Professor

Degree

博士（環境科学）（東北大学）
修士（環境科学）（東北大学）

researchmap

https://researchmap.jp/kaoru.hiramoto

J-GLOBAL ID

202201012586746862

e-Rad No.

40963038

ORCID

https://orcid.org/0000-0002-2210-7971

Research History 4

2022/04 - Present

Tohoku University　Frontier Research Institute for Interdisciplinary Sciences Creative Interdisciplinary Research Division　Assistant Professor
2020/04 - 2022/03

Tohoku University　Graduate School of Environmental Studies　Research Fellowship for Young Scientists
2019/05 - 2020/03

Tohoku University　Graduate School of Engineering
2012/04 - 2019/04

Panasonic Corporation

Education 3

Tohoku University　Graduate School of Environmental Studies

2019/10 - 2022/03
Tohoku University　Graduate School of Environmental Studies

2010/04 - 2012/03
Tohoku University　Faculty of Engineering　Department of Applied Chemistry, Chemical Engineering and Biomolecular Engineering

2006/04 - 2010/03

Committee Memberships 4

The Electrochemical Society of Japan　男女共同参画推進委員会

2024/01 - Present
化学とマイクロ・ナノシステム学会　第48回研究会実行委員

2023/04 - 2023/11
化学とマイクロ・ナノシステム学会　第47回研究会実行委員

2022/11 - 2023/05
化学とマイクロ・ナノシステム学会　第46回研究会若手企画実行委員長

2022/04 - 2022/11

Professional Memberships 3

化学とマイクロ・ナノシステム学会
International Society of Electrochemistry
電気化学会

Research Interests 4

3次元培養細胞
脂質膜
電気化学発光
Electrochemistry

Research Areas 3

Nanotechnology/Materials / Analytical chemistry /
Natural sciences / Bio-, chemical, and soft-matter physics /
Nanotechnology/Materials / Nanobioscience /

Awards 1

Lab on a Chip outstanding research award

2023/11

Papers 33

Electrochemiluminescence of [Ru(bpy)<inf>3</inf>]²⁺/tri-n-propylamine to visualize different lipid compositions in supported lipid membranes

Kaoru Hiramoto, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku

Chemical Communications　61　(23)　4495-4498　2025/02/02

DOI： 10.1039/d4cc06245k 　

ISSN： 1359-7345

eISSN： 1364-548X
Spectroelectrochemistry and light active process at nanointerface: general discussion

Ding, Z., Ewing, A.G., Fu, K.X., Hill, C.M., Hiramoto, K., Kanoufi, F., Kasianowicz, J.J., Kranz, C., Kuttner, C., Lang, S.-Y., Li, F., Liu, L., Lu, J., Mount, A.R., Ren, H., Shao, Y., Shen, M., Takahashi, Y., Wang, W., Xia, F., Zhang, G., Zhao, Z., Zhou, Y.

Faraday Discussions　257　2025

DOI： 10.1039/d4fd90070g 　

ISSN： 1359-6640 1364-5498
Electrochemiluminescence microscopy of diffusive biocompounds as co-reactants in cell spheroids with [Ru(bpy)3]2+

Mockaitis, T., Shikuwa, R., Utagawa, Y., Oba, K., Abe, H., Shiku, H., Ino, K., Morkvenaite-Vilkonciene, I., Hiramoto, K.

Sensors and Actuators B Chemical　441　2025

DOI： 10.1016/j.snb.2025.137944 　

ISSN： 0925-4005
Systems nanoelectrochemistry from single entity to ensemble: general discussion

Actis, P., Aoki, K., Baker, L.A., Caniglia, G., Dang, N., Ding, Z., Ewing, A.G., Fu, K.X., Gooding, J.J., Hiramoto, K., Jiang, D., Kanoufi, F., Kasianowicz, J.J., Kranz, C., Lang, S.-Y., Li, F., Li, H., Liu, L., Long, Y.-T., Lu, J., Lu, S.-M., McKelvey, K., Mount, A.R., Ren, H., Shao, Y., Shen, M., Siwy, Z.S., Takahashi, Y., Tang, J., Wang, H., Wang, J.-G., Wang, K., Wang, L., Wang, Y., Xia, F., Xie, R.-C., Ying, Y.-L., Zhang, G., Zhao, Z.

Faraday Discussions　257　2025

DOI： 10.1039/d4fd90068e 　

ISSN： 1359-6640 1364-5498
Extended Spherical Diffusion Theory: Electrochemiluminescence Imaging Analysis of Diffusive Molecules from Spherical Biosamples Peer-reviewed

Kosuke Ino, Miyu Mashiko, Yusuke Kanno, Yeyi Tang, Shuzo Masui, Takasi Nisisako, Kaoru Hiramoto, Hiroya Abe, Hitoshi Shiku

Analytical Chemistry　96　(48)　18967-18976　2024/11/18
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.4c03167 　

ISSN： 0003-2700

eISSN： 1520-6882
Electrochemical Evaluation of Three-dimensional Cell Models Peer-reviewed

Kaoru HIRAMOTO

Vacuum and Surface Science　67　(9)　450-455　2024/09/10
Publisher: Surface Science Society Japan
DOI： 10.1380/vss.67.450 　

ISSN： 2433-5835

eISSN： 2433-5843
Osteogenic differentiation of mesenchymal stem cell spheroids: A microfluidic device and electrochemiluminescence imaging study Peer-reviewed

Kaoru Hiramoto, An Konno, Yuji Nashimoto, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku

Electrochimica Acta　491　144291-144291　2024/04
Publisher: Elsevier BV
DOI： 10.1016/j.electacta.2024.144291 　

ISSN： 0013-4686
Optical Properties of Ultrathin Biohybrid Membranes: Implications for Optoelectronic Applications

Teng Ma, Kaito Watabe, Maki Komiya, Kaoru Hiramoto, Xingyao Feng, Daisuke Tadaki, Ayumi Hirano-Iwata

ACS Applied Nano Materials　7　(16)　18379-18385　2024/01/23
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acsanm.3c04080 　

ISSN： 2574-0970

eISSN： 2574-0970
Preface to Special Issue on Micro/Nanodevices for Electrochemical Bioanalysis

Kaoru Hiramoto, Fei Li

Electrochemical Science Advances　2024

DOI： 10.1002/elsa.202400044 　

eISSN： 2698-5977
Electrochemiluminescence microscopy for the investigation of peptides interactions within planar lipid membranes Peer-reviewed

Kaoru Hiramoto, Kosuke Ino, Ibuki Takahashi, Ayumi Hirano-Iwata, Hitoshi Shiku

Faraday Discussions　257　137-150　2024
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d4fd00137k 　

ISSN： 1359-6640

eISSN： 1364-5498
Vasculature-on-a-Chip with a Porous Membrane Electrode for In Situ Electrochemical Detection of Nitric Oxide Released from Endothelial Cells Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Kazuyuki Iwase, Yuji Nashimoto, Itaru Honma, Hitoshi Shiku

Analytical Chemistry　95　(49)　18158-18165　2023/11/28
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.3c03684 　

ISSN： 0003-2700

eISSN： 1520-6882
Simple, Rapid, and Large‐Scale Fabrication of Multi‐Branched Hydrogels Based on Viscous Fingering for Cell Culture Applications Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Hitoshi Shiku

Macromolecular Bioscience　23　(9)　2023/04/22
Publisher: Wiley
DOI： 10.1002/mabi.202300069 　

ISSN： 1616-5187

eISSN： 1616-5195
Effects of Ion Concentration on Ion Current Rectification Using a Glass Nanocapillary Peer-reviewed

Chisato ITO, Kosuke INO, Kaoru HIRAMOTO, Yuji NASHIMOTO, Hitoshi SHIKU

BUNSEKI KAGAKU　72　(3)　117-123　2023/03/05
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.72.117 　

ISSN： 0525-1931
Evaluation of respiratory and secretory activities of multicellular spheroids via electrochemiluminescence imaging International-journal Invited Peer-reviewed

Kaoru Hiramoto, Keika Komatsu, Ryota Shikuwa, An Konno, Yusuke Sato, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku

Electrochimica Acta　458　142507-142507　2023
Publisher: Elsevier BV
DOI： 10.1016/j.electacta.2023.142507 　

ISSN： 0013-4686
In vitro electrochemical assays for vascular cells and organs International-journal Invited Peer-reviewed

Utagawa, Y., Hiramoto, K., Nashimoto, Y., Ino, K., Shiku, H.

Electrochemical Science Advances　2　(5)　e2100089　2022/10

DOI： 10.1002/elsa.202100089 　

ISSN： 2698-5977

eISSN： 2698-5977
Electrochemical microwell sensor with Fe–N co-doped carbon catalyst to monitor nitric oxide release from endothelial cell spheroids Peer-reviewed

Kaoru Hiramoto, Kazuyuki Iwase, Yoshinobu Utagawa, Yuji Nashimoto, Itaru Honma, Kosuke Ino, Hitoshi Shiku

Analytical sciences　38　(10)　1297-1304　2022/07/27

DOI： 10.1007/s44211-022-00160-0 　

ISSN： 0910-6340

eISSN： 1348-2246
Electrochemical Glue for Binding Chitosan–Alginate Hydrogel Fibers for Cell Culture Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Tatsuki Kumagai, Kaoru Hiramoto, Masahiro Takinoue, Yuji Nashimoto, Hitoshi Shiku

Micromachines　13　(3)　2022/03

DOI： 10.3390/mi13030420 　

ISSN： 2072-666X
Electrochemiluminescence imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds Peer-reviewed

Ino, K., Komatsu, K., Hiramoto, K., Utagawa, Y., Nashimoto, Y., Shiku, H.

Electrochimica Acta　415　2022

DOI： 10.1016/j.electacta.2022.140240 　

ISSN： 0013-4686
Electrochemical Imaging of Endothelial Permeability Using a Large-Scale Integration-Based Device. International-journal Peer-reviewed

Kosuke Ino, Hao-Jen Pai, Kaoru Hiramoto, Yoshinobu Utagawa, Yuji Nashimoto, Hitoshi Shiku

ACS omega　6　(51)　35476-35483　2021/12/28

DOI： 10.1021/acsomega.1c04931 　

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It is important to clarify the transport of biomolecules and chemicals to tissues. Herein, we present an electrochemical imaging method for evaluating the endothelial permeability. In this method, the diffusion of electrochemical tracers, [Fe(CN)6]4-, through a monolayer of human umbilical vein endothelial cells (HUVECs) was monitored using a large-scale integration-based device containing 400 electrodes. In conventional tracer-based assays, tracers that diffuse through an HUVEC monolayer into another channel are detected. In contrast, the present method does not employ separated channels. In detail, a HUVEC monolayer is immersed in a solution containing [Fe(CN)6]4- on the device. As [Fe(CN)6]4- is oxidized and consumed at the packed electrodes, [Fe(CN)6]4- begins to diffuse through the monolayer from the bulk solution to the electrodes and the obtained currents depend on the endothelial permeability. As a proof-of-concept, the effects of histamine on the monolayer were monitored. Also, an HUVEC monolayer was cocultured with cancer spheroids, and the endothelial permeability was monitored to evaluate the metastasis of the cancer spheroids. Unlike conventional methods, the device can provide spatial information, allowing the interaction between the monolayer and the spheroids to be monitored. The developed method is a promising tool for organs-on-a-chip and drug screening in vitro.
Electrochemiluminescence imaging of respiratory activity of cellular spheroids using sequential potential steps International-journal Peer-reviewed

Kaoru Hiramoto

Biosensors and Bioelectronics　181　113123-113123　2021/06

DOI： 10.1016/j.bios.2021.113123 　

ISSN： 0956-5663

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The respiratory activity of cultured cells can be electrochemically monitored using scanning electrochemical microscopy (SECM) with high spatial resolution. However, in SECM, the electrode takes a long time to scan, limiting simultaneous measurements with large biological samples such as cell spheroids. Therefore, for rapid electrochemical imaging, a novel strategy is needed. Herein, we report electrochemiluminescence (ECL) imaging of spheroid respiratory activity for the first time using sequential potential steps. L-012, a luminol analog, was used as an ECL luminophore, and H2O2, a sensitizer for ECL of L-012, was generated by the electrochemical reduction of dissolved O2. The ECL imaging visualized spheroid respiratory activity-evidenced by ECL suppression-corresponding to O2 distribution around the spheroids. This method enabled the time-lapse imaging of respiratory activity in multiple spheroids with good spatial resolution comparable to that of SECM. Our work provides a promising high-throughput imaging strategy for elucidating spheroid cellular dynamics.
Electrodeposition of Thiolated Polymer-based Hydrogels via Disulfide Formation Using Electrogenerated Benzoquinone Peer-reviewed

Kosuke Ino, Ayako Tamura, Kaoru Hiramoto, Mika T. Fukuda, Yuji Nashimoto, Hitoshi Shiku

Chemistry Letters　50　(2)　256-259　2021/02/05

DOI： 10.1246/cl.200732 　

ISSN： 0366-7022

eISSN： 1348-0715
A droplet array device for electrochemical detection of methylene blue based on local redox cycling Peer-reviewed

Hiramoto, K., Komatsu, K., Yamada, Y., Nashimoto, Y., Matsue, T., Ino, K., Shiku, H.

Bunseki Kagaku　70　(3)　183-189　2021
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.70.183 　

ISSN： 0525-1931
Fabrication of three-dimensional calcium alginate hydrogels using sacrificial templates of sugar. Peer-reviewed

Kosuke Ino, Mika T Fukuda, Kaoru Hiramoto, Noriko Taira, Yuji Nashimoto, Hitoshi Shiku

Journal of bioscience and bioengineering　130　(5)　539-544　2020/11

DOI： 10.1016/j.jbiosc.2020.06.014 　

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Hydrogels are receiving increasing attention in bioapplications. Among hydrogels, calcium alginate (Ca-alginate) hydrogels are widely used for their biocompatibility, low toxicity, low cost, and rapid fabrication by simple mixing of Ca2+ and sodium alginate (Na-alginate). For bioapplications using hydrogels, it is necessary to construct designed hydrogel structures. Although several methods have been proposed for fabricating designed hydrogels, a simple and low-cost method is desirable. Therefore, we developed a new method using sacrificial templates of sugar structures to fabricate three-dimensional (3D) designed Ca-alginate hydrogels. In this method, Na-alginate solution is mixed with molten sugar, and the resulting highly viscous material used to mold 3D sugar structures as sacrificial templates. Since sugar constructs are easily handled compared to hydrogels, sugar templates are useful for preparing 3D constructs. Finally, the sugar and Na-alginate structure is immersed in a CaCl2 solution to simultaneously dissolve the template and form the Ca-alginate hydrogel. The resulting hydrogel takes the shape of the sugar template. By stacking and fusing various sugar structures, such as fibers and blocks, 3D designed Ca-alginate hydrogels can be successfully fabricated. This simple and low-cost method shows excellent potential for application to a variety of bioapplications.
Oxygen consumption rate of tumour spheroids during necrotic-like core formation. International-journal Peer-reviewed

Rei Mukomoto, Yuji Nashimoto, Takato Terai, Takuto Imaizumi, Kaoru Hiramoto, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Hitoshi Shiku

The Analyst　145　(19)　6342-6348　2020/09/28

DOI： 10.1039/d0an00979b 　

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Hypoxia is one of the major hallmarks of solid tumours and is associated with the poor prognosis of various cancers. A multicellular aggregate, termed a spheroid, has been used as a tumour model with a necrotic-like core for more than 45 years. Oxygen metabolism in spheroids has been studied using phosphorescence quenching and oxygen-sensitive electrodes. However, these conventional methods require chemical labelling and physical insertion of the electrode into each spheroid, which may be functionally and structurally disruptive. Scanning electrochemical microscopy (SECM) can non-invasively analyse oxygen metabolism. Here, we used SECM to investigate whether the changes of the internal structure of spheroids affect the oxygen metabolism. We investigated the oxygen consumption rate (OCR) of MCF-7 breast tumour spheroids with and without a necrotic-like core. A numerical simulation was used to describe a method for estimating the OCR of spheroids that settled at the bottom of the conventional culture plates. The OCR per spheroid volume decreased with increasing spheroid radius, indicating the limitation of the oxygen supply to the core of the MCF-7 spheroid. Formation of the necrotic-like core did not affect the oxygen metabolism significantly, implying that the core had minimal contribution to the OCR even before necrosis occurred. OCR analysis using SECM non-invasively monitors the change of oxygen metabolism in tumour spheroids. The approach is promising to evaluate various three-dimensional culture models.
Electrochemical measurement of respiratory activity for evaluation of fibroblast spheroids containing endothelial cell networks Peer-reviewed

Hiramoto, K., Pai, H.-J., Ino, K., Nashimoto, Y., Shiku, H.

Electrochimica Acta　340　2020

DOI： 10.1016/j.electacta.2020.135979 　
Closed Bipolar Electrode Array for On-Chip Analysis of Cellular Respiration by Cell Aggregates International-journal Peer-reviewed

Ino, K., Yaegaki, R., Hiramoto, K., Nashimoto, Y., Shiku, H.

ACS Sensors　5　(3)　740-745　2020

DOI： 10.1021/acssensors.9b02061 　
Biofabrication Using Electrochemical Devices and Systems International-journal Peer-reviewed

Ino, K., Ozawa, F., Dang, N., Hiramoto, K., Hino, S., Akasaka, R., Nashimoto, Y., Shiku, H.

Advanced Biosystems　4　(4)　e1900234　2020

DOI： 10.1002/adbi.201900234 　
Recent advances in electrochemiluminescence-based systems for mammalian cell analysis International-journal Peer-reviewed

Hiramoto, K., Villani, E., Iwama, T., Komatsu, K., Inagi, S., Inoue, K.Y., Nashimoto, Y., Ino, K., Shiku, H.

Micromachines　11　(5)　2020

DOI： 10.3390/mi11050530 　
電気化学計測デバイスを用いた細胞の消費物・分泌物評価 Invited

平本薫, 伊野浩介, 梨本裕司, 珠玖仁

Electrochemistry　87　323-328　2019/12

DOI： 10.5796/denkikagaku.19-FE0032 　
Genipin crosslinking of electrodeposited chitosan/gelatin hydrogels for cell culture Peer-reviewed

Tamura, A., Hiramoto, K., Ino, K., Taira, N., Nashimoto, Y., Shiku, H.

Chemistry Letters　48　(10)　2019

DOI： 10.1246/cl.190466 　
Electric and electrochemical microfluidic devices for cell analysis International-journal Peer-reviewed

Hiramoto, K., Ino, K., Nashimoto, Y., Ito, K., Shiku, H.

Frontiers in Chemistry　7　(JUN)　396-396　2019

DOI： 10.3389/fchem.2019.00396 　
Development of Oxygen Consumption Analysis with an on-Chip Electrochemical Device and Simulation International-journal Peer-reviewed

Hiramoto, K., Yasumi, M., Ushio, H., Shunori, A., Ino, K., Shiku, H., Matsue, T.

Analytical Chemistry　89　(19)　10303-10310　2017

DOI： 10.1021/acs.analchem.7b02074 　
Carbon-Ag/AgCl probes for detection of cell activity in droplets. International-journal Peer-reviewed

Kosuke Ino, Kaoru Ono, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Analytical chemistry　85　(8)　3832-5　2013/04/16

DOI： 10.1021/ac303569t 　

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In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.

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Misc. 3

Recent advances in electrochemiluminescence sensing for in vitro cell analysis: a review

Kosuke Ino, Tomas Mockaitis, Ryota Shikuwa, Kimiharu Oba, Kaoru Hiramoto, Inga Morkvenaite-Vilkonciene, Hiroya Abe, Hitoshi Shiku

Analytical Sciences　2025

DOI： 10.1007/s44211-025-00723-x 　

ISSN： 0910-6340

eISSN： 1348-2246
Porous membranes integrated into electrochemical systems for bioanalysis

Kosuke Ino, Yoshinobu Utagawa, Kaoru Hiramoto, Hiroya Abe, Hitoshi Shiku

Electrochemical Science Advances　4　(6)　2024

DOI： 10.1002/elsa.202300026 　

eISSN： 2698-5977
How Did I Manage Maternal Life Events as a Researcher?

HIRAMOTO Kaoru

Denki Kagaku　90　(3)　298-299　2022/09/05
Publisher: The Electrochemical Society of Japan
DOI： 10.5796/denkikagaku.22-ot0038 　

ISSN： 2433-3255

eISSN： 2433-3263

Presentations 18

電気化学発光による画像ベースのバイオ分析 Invited

平本薫

第34回日本MRS年次大会　2024/12/16
電気化学発光イメージングによる細胞・生体分子活性計測 Invited

平本薫

第55回セミコンファレンス　2024/12/15
ガラスナノピペットによる脂質膜コートの影響

楫千咲恵, 平本薫, 平野愛弓

化学とマイクロ・ナノシステム学会第50回研究会　2024/11/27
Electrochemiluminescence microscopy for the investigation of peptides interactions within planar lipid membranes International-presentation

Faraday Discussions　2024/10/15
脂質膜コートナノピペットの作製とエクソソーム検出

楫千咲恵, 平本薫, 平野愛弓

第85回応用物理学会秋季学術講演会　2024/09/20
脂質二分子膜－表面探索からセンサ応用に向けて－ Invited

平本薫

日本機械学会2024年度年次大会　2024/09/09
脂質膜コーティングナノピペットを用いたエクソソーム検出

楫千咲恵, 平本薫, 平野愛弓

化学とマイクロ・ナノシステム学会第49回研究会　2024/06/02
On the use of Electrochemiluminescence Microscopy: keys to visualizing biological phenomena International-presentation Invited

Kaoru Hiramoto

International Virtual Nanopore Weekly Meeting　2023/11/20
脂質二分子膜の新規電気化学発光イメージング

平本薫, 伊野浩介, 平野愛弓, 珠玖仁

化学とマイクロ・ナノシステム学会第48回研究会　2023/11/06
Electrochemical measurement techniques for the evaluation of 3D cell models Invited

Kaoru Hiramoto

The Japan Society of Vaccum and Surface Science　2023/11/02
なぜ私は大学で研究者になったのか Invited

平本薫

東北大学附置研究所等一般公開片平まつり2023　2023/10/07
Developing electrochemiluminescence imaging method for the study of supported lipid bilayers International-presentation

Kaoru Hiramoto, Kosuke Ino, Ayumi Hirano-Iwata, Hitoshi Shiku

74th Annual Meeting of the International Society of Electrochemistry　2023/09/05
細胞活性測定のための電気化学インターフェース Invited

平本薫

第11回Chem-Bio Joint Seminar　2023/08/04
Electrochemiluminescence Imaging of Respiratory Activity and Cellular Secretion of Three-Dimensional Cultured Cells

Kosuke Ino, Kaoru Hiramoto, Keika Komatsu, Yuji Nashimoto, Hitoshi Shiku

73rd Annual Meeting of the International Society of Electrochemistry　2022/09/15
Fe and N co-doped carbon catalyst modified electrode for real-time monitoring of nitric oxide release from cells

Kaoru Hiramoto, Kazuyuki Iwase, Yoshinobu Utagawa, Yuji Nashimoto, Itaru Honma, Kosuke Ino, Hitoshi Shiku

2021/11/22
HIGH RESOLUTION, ONE-SHOT IMAGING OF CELLULAR ACTIVITY OF SPHEROIDS BY OXYGEN DEPENDENT ELECTROCHEMILUMINESCENCE

Kaoru Hiramoto, Kosuke Ino, Keika Komatsu, Yuji Nashimoto, Hitoshi Shiku

MicroTAS 2021　2021/10/12
Electrochemiluminescence Imaging of Cell Spheroid and its Ex Vivo Microenvironment

Kaoru Hiramoto, Kosuke Ino, Keika Komatsu, Yuji Nashimoto, Hitoshi Shiku

2020/12/10
Effect of endothelial cell networks on respiratory activity of spheroids

Kaoru Hiramoto, Hao-Jen Pai, Kosuke Ino, Yuji Nashimoto, Hitoshi Shiku

71st Annual Meeting of the International Society of Electrochemistry　2020/08/31

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Industrial Property Rights 3

電気化学測定方法および電気化学測定装置

平本薫, 牛尾浩司, 安見正博

特許第6920642号

Property Type: Patent
電気化学測定システム、電気化学測定装置および電気化学測定方法

平本薫, 安見正博

特許第6492294号

Property Type: Patent
電気化学測定方法および電気化学測定装置

平本薫, 安見正博, 牛尾浩司, 守法篤

特許第6078799号

Property Type: Patent

Research Projects 8

Development of biomedical wetwares based on artificial neuronal networks

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Transformative Research Areas (A)

Institution: Tohoku University

2024/04/01 - 2029/03/31
神経細胞回路の電気・化学シグナル伝搬の相互可視化

Offer Organization: 上原記念生命科学財団

System: 研究奨励金

2024/04 - 2026/03
Modeling neurodegenerative diseases in artificial neuronal networks

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Institution: Tohoku University

2022/10 - 2026/03
センサ応用に向けた3次元脂質二分子膜評価系の開発

平本薫

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 若手研究

Institution: 東北大学

2023/04/01 - 2025/03/31
電気化学発光を利用した新規生体膜機能解析手法の開発

Offer Organization: 花王芸術・科学財団

System: 花王Crescentaward

2023/04 - 2025/03
3次元電気化学発光顕微鏡の開発

平本薫

Offer Organization: 公益財団法人旭硝子財団

System: 化学・生命分野研究奨励

2023/04 - 2025/03
生体膜機能を可視化する電気化学発光顕微鏡の創製

平本薫

Offer Organization: 日本学術振興会

System: 科学研究費助成事業研究活動スタート支援

Category: 研究活動スタート支援

Institution: 東北大学

2022/08 - 2024/03
電気化学とOrgan-on-a-chipの融合によるがん腫瘍モデル評価基盤の構築

平本薫

Offer Organization: 日本学術振興会

System: 科学研究費助成事業特別研究員奨励費

Category: 特別研究員奨励費

Institution: 東北大学

2020/04/24 - 2023/03/31

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三次元培養した細胞塊（スフェロイド）は生体に近い機能を持つため、モデル組織として移植医療や創薬への応用研究が推進されている。電気化学を利用した細胞組織評価基盤の確立のために、2つのアプローチをとった。①高度な生体組織モデルの作製および電気化学計測による機能評価と、②高解像度かつハイスループット測定を可能とする電気化学デバイスの開発である。 ①生体外で大きな細胞構造体を構築しようとすると、内部まで酸素や栄養素が供給されず細胞死が生じるという問題がある。細胞スフェロイドに血管構造を組みこむことで物質輸送が改善され、より生体組織の再現性が高まると考えられる。そこで血管内皮細胞と繊維芽細胞の共培養により血管様の細胞ネットワークを有するスフェロイドを作製し、走査型電気化学顕微鏡（SECM）および多点電極アレイデバイスを用いて、電気化学的な呼吸測定によりその機能評価を行った。作製したスフェロイドに、代表的な抗がん剤であるドキソルビシンを添加すると、血管内皮細胞の割合が大きいスフェロイドほど呼吸量が顕著に減少していることがわかった。血管構造によって薬剤浸透性が高まったことが示唆される。 ②代表的な電気化学測定ツールであるSECMは1本のプローブを走査するという特性上、高空間分解能であるがスループット性が低い。そこでスループットを向上させるアプローチとして、電気化学発光（Electrochemiluminescence: ECL）を利用した呼吸活性イメージングデバイスを作製した。1枚の平板電極上で酸素の還元と発光物質の酸化を交互に行うことで微小領域の酸素濃度を発光反応に変換するシステムを構築し、ミニ軟骨組織およびがん腫瘍モデルの複数同時の呼吸イメージングに成功した。ECLイメージングによってSECMと同程度の空間分解能を有しながら、スループット性を大幅に向上させることが可能となった。

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