Details of the Researcher

PHOTO

Tokiha Ozawa
Section
Graduate School of Life Sciences
Job title
Degree
  • Ph.D. (Science) (Yokohama City University)

e-Rad No.
30866330

Research History 7

  • 2024/04 - Present
    Tohoku University Graduate School of Life Sciences JSPS Research Fellow

  • 2021/09 - 2024/03
    Tohoku University Graduate School of Life Sciences

  • 2020/07 - 2021/08
    Tohoku University Frontier Research Institute for Interdisciplinary Sciences Creative Interdisciplinary Research Division

  • 2019/05 - 2020/03
    The University of Tokyo Research Center for Advanced Science and Technology

  • 2010/02 - 2012/06
    University of Illinois at Urbana-Champaign Department of Biochemistry Research associate

  • 2009/04 - 2010/01
    RIKEN

  • 2005/04 - 2008/03
    RIKEN

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Education 3

  • Yokohama City University

    2004/04 - 2009/03

  • Yokohama City University

    2002/04 - 2004/03

  • Japan Women's University Faculty of Science

    1998/04 - 2002/03

Research Interests 5

  • 標的タンパク質分解

  • PROTAC

  • DNA修復

  • 相同組換え

  • タンパク質

Research Areas 2

  • Nanotechnology/Materials / Chemical biology /

  • Life sciences / Molecular biology /

Papers 8

  1. Indirect ubiquitination independent of endogenous ubiquitination machinery for targeted protein degradation

    Takafumi Furuhata, Kazuki Yoshida, Ryoka Fujita, Jotaro Miyamoto, Chiharu Moriyama, Tokiha Masuda-Ozawa, Hikaru Tsuchiya, Yasushi Saeki, Akimitsu Okamoto

    Communications Chemistry 2026/01/29

    DOI: 10.1038/s42004-026-01895-x  

    ISSN: 2399-3669

  2. Well-to-well transfer of HaloTag ligand 6-chlorohexan-1-ol and formaldehyde in a multi-well plate

    Yugo Mishima, Saori Yasuda, Aoi Kunitomi, Tokiha Masuda-Ozawa, Shusuke Tomoshige, Shunji Kato, Yuki Kadono, Kiyotaka Nakagawa, Minoru Ishikawa

    Scientific Reports 2025/11/29

    DOI: 10.1038/s41598-025-29372-w  

    ISSN: 2045-2322

    More details Close

    <jats:title>Abstract</jats:title> <jats:p>When volatile organic compounds are handled in multi-well plates, their volatilization is known to affect non-treated wells in the plate. In this paper, we report two cases in which the HaloTag ligand 6-chlorohexan-1-ol and formaldehyde were volatilized and transferred to other wells in a multi-well plate. We found that competition between the HaloTag-targeting PROTAC and 6-chlorohexan-1-ol affected the other wells of the plate. A multifaceted analysis revealed that this phenomenon resulted from well-to-well transfer of 6-chlorohexan-1-ol into untreated wells on the plate. In addition, the formaldehyde used for cell fixation was toxic to cells in the surrounding wells. Therefore, even these reagents, which are widely used in life science research, require careful handling in multi-well plates.</jats:p>

  3. siRNA-mediated gene knockdown via electroporation in hydrozoan jellyfish embryos

    Tokiha Masuda-Ozawa, Sosuke Fujita, Ryotaro Nakamura, Hiroshi Watanabe, Erina Kuranaga, Yu ichiro Nakajima

    Scientific Reports 12 (1) 2022/12

    DOI: 10.1038/s41598-022-20476-1  

    eISSN: 2045-2322

  4. Nonfilament-forming RecA dimer catalyzes homologous joint formation

    Takeshi Shinohara, Naoto Arai, Yukari Iikura, Motochika Kasagi, Tokiha Masuda-Ozawa, Yuuki Yamaguchi, Kayo Suzuki-Nagata, Takehiko Shibata, Tsutomu Mikawa

    Nucleic Acids Research 46 (20) 10855-10869 2018

    DOI: 10.1093/nar/gky877  

    ISSN: 0305-1048

    eISSN: 1362-4962

  5. Single-molecule sorting reveals how ubiquitylation affects substrate recognition and activities of FBH1 helicase

    Tokiha Masuda-Ozawa, Trish Hoang, Yeon Soo Seo, Lin Feng Chen, Maria Spies

    Nucleic Acids Research 41 (6) 3576-3587 2013/04

    DOI: 10.1093/nar/gkt056  

    ISSN: 0305-1048

    eISSN: 1362-4962

  6. Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein

    Tokiha Masuda, Feng Ling, Takehiko Shibata, Tsutomu Mikawa

    FEBS Journal 277 (6) 1440-1452 2010/03

    DOI: 10.1111/j.1742-4658.2010.07574.x  

    ISSN: 1742-464X

    eISSN: 1742-4658

  7. A non-canonical DNA structure enables homologous recombination in various genetic systems

    Tokiha Masuda, Yutaka Ito, Tohru Terada, Takehiko Shibata, Tsutomu Mikawa

    Journal of Biological Chemistry 284 (44) 30230-30239 2009/10/30

    DOI: 10.1074/jbc.M109.043810  

    ISSN: 0021-9258

    eISSN: 1083-351X

  8. Purification and characterization of the Thermus thermophilus HB8 RecX protein

    Koutaro Jimbo, Jin Inoue, Tokiha Masuda, Takehiko Shibata, Tsutomu Mikawa

    Protein Expression and Purification 51 (2) 320-323 2007/02

    DOI: 10.1016/j.pep.2006.09.001  

    ISSN: 1046-5928

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Research Projects 2

  1. 神経変性疾患の原因凝集性タンパク質を分解する新規分解薬の創製

    小澤 ときは

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 特別研究員奨励費

    Institution: 東北大学

    2024/04/01 - 2027/03/31

  2. 転写因子を標的とした新規プロテインノックダウン法の開発

    小澤 ときは

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 若手研究

    Institution: 東京大学

    2020/04/01 - 2022/03/31