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Hiroshi Egusa

Section

Graduate School of Dentistry

Job title

Professor

Degree

博士（歯学）（広島大学）

researchmap

https://researchmap.jp/HiroshiEgusa

J-GLOBAL ID

200901019174016632

Research History 26

2025/01 - Present

Chulalongkorn University,　Faculty of Dentistry　Honorary Professor
2023/04 - Present

Tohoku University Graduate School of Dentistry　Joint Research Department of Next-Generation Dental Material Engineering　Professor
2022/04 - Present

Tohoku University Hospital　General Vice-Director
2022/04 - Present

Head of Dental Division, Tohoku University Hospital
2020/04 - Present

Tohoku University Graduate School of Dentistry　Center for Advanced Stem Cell and Regenerative Research　Director
2014/04 - Present

Tohoku University Graduate School of Dentistry　Division of Molecular and Regenerative Prosthodontics　Professor and Chair
2018/04 - 2022/03

Tohoku University Hospital　Vice-Director
2007/04 - 2014/03

Osaka University　Graduate School of Dentistry
2004/04 - 2007/03

Osaka University　Graduate School of Dentistry
2002/06 - 2004/05

University of California, Los Angels (UCLA), The Jane and Jerry Weintraub Center for Reconstructive Biotechnology　Visiting Scholar (Postdoctoral Research Fellow)
2002/04 - 2004/03

Japan Society for the Promotion of Science (JSPS)　Japanese Government Special Postdoctoral Research Fellow
1999/01 - 1999/07

University of Hong Kong, Department of Oral Microbiology　Technical Assistant
2024/06 - Present

東北大学特定認定再生医療等委員会副委員長
2024/06 - Present

東北大学認定再生医療等委員会副委員長
2023/04 - Present

Institute of Science Tokyo (formerly Tokyo Institute of Technology)　Institute of Innovative Research　Visiting Professor
2022/04 - Present

東北大学　教育研究評議会　評議員
2022/04 - Present

Tohoku University Hospital　Dental Safety and System Management　Chair
2021/04 - Present

Tohoku University Hospital　Dental Laboratory Unit　Chief
2016/04 - Present

Hiroshima University　Faculty of Dentistry　Visiting Professor
2015/04 - Present

Showa University　School of Dentistry　Visiting Professor
2014/04 - Present

Tohoku University Hospital　Fixed Prosthodontics　Chair
2018/04 - 2023/03

Tohoku University　Provost Staff
2018/04 - 2023/03

Tohoku University Graduate School of Dentistry　Liaison Center for Innovative Dentistry Director　Director
2021/04 - 2022/03

Tohoku University Hospital　Dental Hygiene Unit　Chief
2018/04 - 2021/03

Tohoku University Hospital　Infection Control Unit (Dentistry)　Chair
2001/05 - 2002/03

Hiroshima University　Graduate School of Dental Science

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Education 2

Hiroshima University　Graduate School　PhD: Division of Dental Science

1998/04 - 2002/03
Hiroshima University　DDS: Faculty of Dentistry

1992/04 - 1998/03

Committee Memberships 42

Japanese Association for Dental, Oral, and Craniofacial Research (JADR)　Vice-President (President-Elect)

2025/01 - Present
日本歯科専門医機構　共通研修企画実施委員会委員

2024/07 - Present
東北大学特定認定再生医療等委員会　副委員長

2024/06 - Present
東北大学認定再生医療等委員会　副委員長

2024/06 - Present
Japanese Stomatological Society　Director

2024/06 - Present
Oral Frontier Science　Mentor

2024/05 - Present
The Japanese Society for Dental Materials and Devices　Director

2024/04 - Present
Japanese Society for Biomaterials　Director

2024/04 - Present
Japan Association for Oral Infectious Diseases　Director

2023/11 - Present
Science Council of Japan　Associate Members

2023/10 - Present
Frontiers in Physiology (Craniofacial Biology and Dental Research)　Associate Editor

2022/06 - Present
Japanese Society of Oral Implantology　代議員

2022/06 - Present
Japan Prosthodontic Society　JPR Editorial Committee, Chair

2021/06 - Present
Journal of Prosthodontic Research (2022 IF: 3.6, Q1)　Editor-in-Chief

2021/06 - Present
厚生労働省　歯科医師試験委員

2021/04 - Present
Japanese Association for Dental Research (JADR)　Director

2021/01 - Present
International Journal of Oral Science　Editorial Board

2019/10 - Present
先端歯学国際教育研究ネットワーク　歯学政策研究プラットフォーム委員

2019/04 - Present
Journal of Artificial Organs　Editorial Board

2017/06 - Present
日本歯科理工学会　代議員

2016/04 - Present
日本再生医療学会　代議員

2014/11 - Present
日本補綴歯科学会　代議員

2013/04 - Present
Journal of Investigative and Clinical Dentistry　Editorial Board (Associate Editor)

2011/09 - Present
文部科学省大学設置・学校法人審議会（設置計画履行状況等調査委員会）　専門委員

2022/04 - 2024/03
日本バイオマテリアル学会　評議員

2018/04 - 2024/03
日本歯科医学会　学術研究委員会委員

2019/07 - 2021/06
東北大学歯学会　理事

2018/06 - 2021/06
日本補綴歯科学会　学術委員会副委員長

2017/06 - 2021/06
文部科学省大学設置・学校法人審議会（歯学専門委員会）　専門委員

2018/11 - 2020/10
IADR Young Investigator Award Committee　Member

2016/06 - 2019/06
Journal of Prosthodontic Research　: Editorial Board (2013-2017 Senior Scientific Editor)

2011/04 - 2019/05
国際歯科研究学会日本支部（JADR）　理事（Hatton Award選考委員長）

2015/01 - 2018/12
医薬品医療機器総合機構（PMDA）　専門委員

2016/04 - 2018/03
日本学術振興会科学研究費委員会　専門委員

2017/06 - 2017/11
日本補綴歯科学会　Journal of Prosthodontic Research 編集委員会委員

2011/04 - 2017/06
日本学術振興会科学研究費委員会　専門委員

2016/06 - 2016/11
文部科学省　歯学教育の改善・充実に関する調査研究協力者会議フォローアップ小委員会委員

2014/10 - 2016/03
日本学術振興会科学研究費委員会　専門委員

2013/12 - 2014/11
日本学術振興会科学研究費委員会　専門委員

2012/12 - 2013/11
Annual Scientific Meeting of Japanese Society of Oral Implantology Tohoku-Hokkaido Branch　Congress President

2025/09 -
Future Leaders in Prosthodontics (FLiP) 14　Chair, Local Organization Committee

2025/05 -
Annual Scientific Meeting of Japan Prosthodontic Society Tohoku-Hokkaido Branch　Congress President

2017/10 -

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Professional Memberships 11

日本口腔感染症学会
THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE
THE JAPANESE SOCIETY FOR BIOMATERIALS
International College of Prosthodontists
JAPANESE DENTAL EDUCATION ASSOCIATION
JAPANESE SOCIRTY OF ORAL IMPLANTOLOGY
日本歯科理工学会
日本骨代謝学会
歯科基礎医学会
日本補綴歯科学会
International Association for Dental Research（IADR）

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Research Interests 7

バイオマテリアル
口腔インプラント学
歯科補綴学
再生医療
院内感染防止対策
デジタル歯科
骨生物学

Research Areas 3

Life sciences / Regenerative dentistry and dental engineering /
Life sciences / Prosthodontics /
Life sciences / Molecular biology /

Awards 20

Academic Award

2025/05　Japan Prosthodontic Scociety
JPR Best Paper Award（Review Article）

2025/05　Japan Prosthodontic Scociety
JPR Best Paper Award (Original Article)

2023/05　Japan Prosthodontic Society
JPR Best Paper Award（Review Article）

2020/06　Japan Prosthodontic Society
Distinguished Paper Award

2019/05　Japan Prosthodontic Society
科研費審査委員表彰

2014/10　日本学術振興会
Distinguished Achievement and Contributions Award

2013/08　Osaka University
Distinguished Scientist Award (Young Investigator Award)

2012/06　International Association for Dental Research (IADR)
Distinguished Achievement and Contributions Award for Research

2011/08　Osaka University
Award for Distinguished Research in the Oral Sciences

2011/03　Advances in Oral Frontier Science
Distinguished Faculty Award for Research (Yumikura Award)

2010/11　Osaka University School of Dentistry
Best Oral Presentation Award (1st Prize)

2010/11　Asian Academy of Osseointegration
Outstanding Poster Presentation Award

2010/10　Japanese Association of Regenerative Dentistry
Outstanding Research Publication Award

2010/06　Japan Prosthodontic Society
Outstanding Poster Presentation Award

2009/07　Japanese Society for Bone and Mineral Research
Best Poster Presentation Award

2009/04　Asian Academy of Prosthodontics
Winner, Oral Presentation Award Competition

2004/05　Japan Prosthodontic Society
First Place Winner, Arthur R. Frechette Research Award Competition in Prosthodontics

2004/03　International Association for Dental Research (IADR)
First Place Winner, Edward H. Hatton Awards Competition (Senior Category)

2002/03　International Association for Dental Research (IADR)
Unilever Division Travel Award (finalist of JADR)

2001/07　Japanese Association for Dental Research (JADR)

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Papers 220

Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells Peer-reviewed

Kyawsoewin M., Manokawinchoke J., Termkwanchareon C., Lwin HY, Yaklai S., Limjeerajarus N., Limjeerajarus CN, Egusa H., Osathanon T., Limraksasin P.

Sci Rep　15　(1)　26589　2025/07
Inhibition of histone deacetylase 3 in dental mesenchyme regulates the development of tooth root Peer-reviewed

Niibe K., Begun DL, Doi-Fujimura K., Nagasaki A., Zars E., Li X., Taylor EL, MacDougall MB, Egusa H., Westendorf JJ

J Bone Miner Res　2025/07
Shear Stress Regulates Osteogenic Differentiation of Human Dental Pulp Stem Cells via the p38 Pathway Peer-reviewed

Hnin Yu Lwin, Watcharaphol Tiskratok, Maythwe Kyawsoewin, Jeeranan Manokawinchoke, Chutimon Termkwanchareon, Nuttapol Limjeerajarus, Chalida Nakalekha Limjeerajarus, Hiroshi Egusa, Thanaphum Osathanon, Phoonsuk Limraksasin

Int J Mol Sci　2025/06/13

DOI： 10.3390/ijms26125667 　
Removable partial denture, complete denture, and fixed partial denture use and health-related quality of life among older adults with tooth loss: A JAGES cross-sectional study Peer-reviewed

Hoshi-Harada M., Takeuchi K., Kusama T., Aida J., Egusa H., Osaka K.

J Prosthodont Res　2025/06
Production and Biobanking of Dental Stem Cells for Clinical Applications in Regenerative Dentistry: Current Practices and Future Perspectives – A Narrative Review Peer-reviewed

Shuntaro Yamada, Christoph Malkmus, Elise Aasebø, Kamal Mustafa, Hiroshi Egusa, Ana Angelova Volponi

J Dent　2025/06

DOI： 10.1016/j.jdent.2025.105934 　
Moderate heating of waterline improves dental unit water quality by activating bactericidal properties of tap water Peer-reviewed

Fushimi K., Yamada M., Watanabe J., Washio J., Takahashi N., Egusa H.

J Dent Sci　2025/06

DOI： 10.1016/j.jds.2025.05.023 　
Shaking culture attenuates circadian rhythms in induced pluripotent stem cells during osteogenic differentiation through the TEAD-Fbxl3-CRY axis Peer-reviewed

Yunyu Fu, Hiroko Okawa, Naruephorn Vinaikosol, Satomi Mori, Phoonsuk Limraksasin, Praphawi Nattasit, Yu Tahara, Hiroshi Egusa

Cell Death Discovery　11　(1)　252　2025/05/24

DOI： 10.1038/s41420-025-02533-6 　
Shear stress preconditioning enhances periodontal ligament stem cell survival Peer-reviewed

Suwittayarak R., Klincumhom N., Phrueksotsai C., Limjeerajarus N., Limjeerajarus CN, Egusa H., Osathanon T.

Arch Oral Biol　173　106232-106232　2025/05
Publisher: Elsevier BV
DOI： 10.1016/j.archoralbio.2025.106232 　

ISSN： 0003-9969
Mechanical force induces osteogenic differentiation of murine induced pluripotent stem cells via TGF-β signalling Peer-reviewed

Manokawinchoke J., Limraksasin P., Limjeerajarus CN, Limjeerajarus N., Samaranayake LP, Egusa H., Osathanon T.

Orthod Craniofac Res　2025/04
CAD-CAM-fabricated zirconia removable dental prosthesis with telescopic crowns: A case report Peer-reviewed

KondoT., Bhattarai S., Hatakeyama M., Egusa H.

Int J Prosthodont　2025/04

DOI： 10.11607/ijp.9262 　
Effect of Octacalcium Phosphate on Osteogenic Differentiation of Induced Pluripotent Stem Cells in a 3D Hybrid Spheroid Culture Peer-reviewed

Yuki Sugai, Ryo Hamai, Yukari Shiwaku, Takahisa Anada, Kaori Tsuchiya, Tai Kimura, Manami Tadano, Kensuke Yamauchi, Tetsu Takahashi, Hiroshi Egusa, Osamu Suzuki

Biomimetics　10　(4)　205-205　2025/03/26
Publisher: MDPI AG
DOI： 10.3390/biomimetics10040205 　

eISSN： 2313-7673

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Octacalcium phosphate (OCP) has been shown to exhibit an osteogenic property and, therefore, has been utilized recently as a bone substitute, clinically. However, the stimulatory capacity for induced pluripotent stem (iPS) cells is not known. This study investigated whether OCP enhances osteoblastic differentiation of three-dimensionally cultured spheroids of iPS cells compared to hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). Mouse iPS cells were mixed with smaller (less than 53 μm) or larger (300–500 μm) sizes of calcium phosphate (CaP) granules and cultured in a laboratory-developed oxygen-permeable culture chip under minimizing hypoxia for up to 21 days. Osteoblastic differentiation was estimated by the cellular alkaline phosphatase (ALP) activities. The degree of supersaturation (DS) with respect to CaP phases was determined from the media chemical compositions. Incubated CaP materials were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The culture promoted well the formation of hybrid spheroids of CaP materials and iPS cells regardless of the type of materials and their granule sizes. The ALP activity of OCP was about 1.5 times higher than that of β-TCP and HA in smaller granule sizes. FTIR, XRD, and DS analyses showed that larger OCP granules tended to hydrolyze to HA slightly faster than smaller granules with time while HA and β-TCP materials tended to remain unchanged. In conclusion, the results suggest that OCP enhances the osteogenic differentiation of iPS cells more than HA and β-TCP through a mechanism of hydrolyzing to HA. This inherent material property of OCP is essential for enhancing the osteoblastic differentiation of iPS cells.
Postoperative rehabilitation with a CAD/CAM-fabricated occlusal splint following plate reconstruction after hemimandibulectomy and condylectomy: A case report International-journal Peer-reviewed

Tagaino R., Sato N., Yoda N., Koyama S., Egusa H.

Int J Prosthodont　25　(2)　264-267　2025/03

DOI： 10.11607/ijp.8904 　

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Mandibular deviation (MD) is a common reconstruction sequela after segmental mandibulectomy. Although proper postoperative rehabilitation is critical for MD management and minimization, the information available is limited. This report describes postoperative rehabilitation with an occlusal splint fabricated using computer-aided design (CAD) and computer-aided manufacturing (CAM) (CAD/CAM-OS) and the results of a threedimensional occlusal analysis using an intraoral scanner after hemimandibulectomy and plate reconstruction. Despite the short follow-up, adherence to postoperative rehabilitation with CAD/CAM-OS for MD correction, even during radiotherapy, was demonstrated by the digital workflow and analysis results.
Scalable production of anti-inflammatory exosomes from three-dimensional cultures of canine adipose-derived mesenchymal stem cells: production, stability, bioactivity, and safety assessment Peer-reviewed

Thongsit A., Oontawee S., Siriarchavatana P., Rodprasert W., Somparn P., Na Nan D., Osathanon T., Egusa H., Sawangmake C.

BMC Vet Res　21　(1)　81　2025/02

DOI： 10.1186/s12917-025-04517-1 　
Three-dimensional in vivo and finite element analyses of peri-implant bone remodeling after superstructure placement Peer-reviewed

Assoratgoon I., Wan B., Tenkumo T., Sato T., Kawata T., Hardani Putra R., Wu C., Egusa H., Li Q., Sasaki K., Nobuhiro Y.

J Prosthet Dent　133　(6)　1535-1544　2025/02

DOI： 10.1016/j.prosdent.2025.01.015 　
Establishment of subcutaneous transplantation platform for delivering induced pluripotent stem cell-derived insulin-producing cells Peer-reviewed

Tran HT, Rodprasert W., Padeta I., Oontawee S., Purbantoro SD, Thongsit A., Siriarchavatana P., Srisuwatanasagul S., Egusa H., Osathanon T., Sawangmake C.

PLoS One　20　(1)　e0318204-e0318204　2025/01/30
Publisher: Public Library of Science (PLoS)
DOI： 10.1371/journal.pone.0318204 　

eISSN： 1932-6203

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Potential trend of regenerative treatment for type I diabetes has been introduced for more than a decade. However, the technologies regarding insulin-producing cell (IPC) production and transplantation are still being developed. Here, we propose the potential IPC production protocol employing mouse gingival fibroblast-derived induced pluripotent stem cells (mGF-iPSCs) as a resource and the pre-clinical approved subcutaneous IPC transplantation platform for further clinical confirmation study. With a multi-step induction protocol, the functional and matured IPCs were generated by 13 days with a long-term survival capability. Further double encapsulation of mGF-iPSC-derived IPCs (mGF-iPSC-IPCs) could preserve the insulin secretion capacity and the transplantation potential of the generated IPCs. To address the potential on IPC transplantation, a 2-step subcutaneous transplantation procedure was established, comprising 1) vascularized subcutaneous pocket formation and 2) encapsulated IPC bead transplantation. The in vivo testing confirmed the safety and efficiency of the platform along with less inflammatory response which may help minimize tissue reaction and graft rejection. Further preliminary in vivo testing on subcutaneous IPC-bead transplantation in an induced type I diabetic mouse model showed beneficial trends on blood glucose control and survival rate sustainability of diabetic mice. Taken together, an established mGF-iPSC-IPC generation protocol in this study will be the potential backbone for developing the iPSC-derived IPC production employing human and animal cell resources. As well as the potential further development of IPC transplantation platform for diabetes treatment in human and veterinary practices using an established subcutaneous encapsulated IPC-bead transplantation platform presented in this study.
Small extracellular vesicles derived from sequential stimulation of canine adipose-derived mesenchymal stem cells enhance anti-inflammatory activity Peer-reviewed

Oontawee S., Siriarchavatana P., Rodprasert W., Padeta I., Pamulang YV, Somparn P., Pisitkun T., Nambooppha B., Sthitmatee N., Na Nan D., Osathanon T., Egusa H., Sawangmake C.

BMC Vet Res　21　(1)　2025/01/21
Publisher: Springer Science and Business Media LLC
DOI： 10.1186/s12917-024-04465-2 　

eISSN： 1746-6148
Fabrication of hard tissue constructs from induced pluripotent stem cells for exploring mechanisms of hereditary tooth/skeletal dysplasia Peer-reviewed

Kondo T., Thaweesapphithak S., Ambo S., Otake K., Ohori-Morita Y., Mori S., Vinaikosol N., Porntaveetus T., Egusa H.

Int J Mol Sci　26　(2)　804　2025/01/18

DOI： 10.3390/ijms26020804 　
Influence of dual-cure resin-cement curing modes on gingival cytotoxicity and inflammatory responses Peer-reviewed

Kondo T., Kakinuma H., Ambo S., Otake K., Sato Y., Egusa H.

J Dent Sci　20　(1)　586-595　2025/01
Publisher: Elsevier BV
DOI： 10.1016/j.jds.2024.04.019 　

ISSN： 1991-7902
Reduction of aerosol and droplet dispersions using intraoral and extraoral vacuums for dental treatments with face-up, diagonal and upright positions Peer-reviewed

Tomonari Kajita, Kenji Kikuchi, Hiromitsu Morishima, Jun Watanabe, Yoko Iwamatsu-Kobayashi, Hiroyasu Kanetaka, Wataru Yashiro, Kensuke Yamauchi, Hiroshi Egusa

BMC Oral Health　24　(1)　2024/11/17
Publisher: Springer Science and Business Media LLC
DOI： 10.1186/s12903-024-04911-5 　

eISSN： 1472-6831
Anisotropic patterns of nanospikes induces anti-biofouling and mechano-bactericidal effects of titanium nanosurfaces with electrical cue Peer-reviewed

Eiji Kato, Masahiro Yamada, Eitoyo Kokubu, Hiroshi Egusa, Kazuyuki Ishihara

Materials Today Bio　2024/11

DOI： 10.1016/j.mtbio.2024.101352 　
Current perspectives on the dynamic culture of mesenchymal stromal/stem cell spheroids Peer-reviewed

Ohori-Morita Y., Ashry A., NiibeK., Egusa H.

Stem Cells Transl Med　accepted　2024/11

DOI： 10.1093/stcltm/szae093 　
Effects of inorganic phosphate on stem cells isolated from human exfoliated deciduous teeth Peer-reviewed

Suwittayarak R., Nowwarote N., Kornsuthisopon C., Sukarawan W., Foster BL, Egusa H., Osathanon T.

Sci Rep　14　(1)　24282　2024/10
Effects of flushing of dental waterlines in portable dental units on water quality management Peer-reviewed

Fushimi K., Watanabe J., Yamada M., Washio J., Takahashi N., Egusa H.

J Dent Sci　19　S61-S69　2024/10

DOI： 10.1016/j.jds.2024.10.014 　
Induced pluripotent stem cell-derived neural stem cells promote bone formation in mice with calvarial defects Peer-reviewed

Yukari Shiwaku, Hiroko Okawa, Ikuro Suzuki, Susumu Sakai, Hiroshi Egusa, Osamu Suzuki

Acta Biomaterialia　188　93-102　2024/10
Publisher: Elsevier BV
DOI： 10.1016/j.actbio.2024.08.054 　

ISSN： 1742-7061
Titanium nanotopography enhances mechano-response of osteocyte three-dimensional network toward osteoblast activation

Xindie He, Masahiro Yamada, Jun Watanabe, Qu Pengyu, Jiang Chen, Hiroshi Egusa

Biomaterials Advances　163　213939-213939　2024/10
Publisher: Elsevier BV
DOI： 10.1016/j.bioadv.2024.213939 　

ISSN： 2772-9508
Intermittent compressive force regulates matrix metalloproteinases and tissue inhibitors of metalloproteinases expression in human periodontal ligament cells Peer-reviewed

Pakpahan ND, Kyawsoewin M., Manokawinchoke J., Namangkalakul W., Termkwancharoen C., Egusa H., Limraksasin P., Osathanon T.

Arch Oral Biol　165　213939　2024/09

DOI： 10.1016/j.archoralbio.2024.106011 　
Mechanical force modulates inflammation and immunomodulation in periodontal ligament cells

Chansaenroj J., Suwittayarak R., Egusa H., Samaranayake LP, Osathanon T.

Medical Review　4　(6)　544-548　2024/08/13
Publisher: Walter de Gruyter GmbH
DOI： 10.1515/mr-2024-0034 　

eISSN： 2749-9642

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Abstract Mechanical forces control a multitude of biological responses in various cells and tissues. The periodontal ligament, located between the tooth’s root and alveolar bone, is a major tissue compartment that is incessantly subjected to such mechanical stimulation through either normal or abnormal oral functionality. It is now known that mechanical stimulation activates periodontal ligament stem cells (PDLSCs) to modulate periodontal immunity and regulate inflammation – a basic feature of periodontal disease that affects virtually every human during their lifetime. For instance, shear stress induces the expression of immunomodulatory-related gene, indoleamine 2,3-dioxygenase (IDO). IDO cleaves l-tryptophan, resulting in increased l-kynurenine levels that, in turn, further promote regulatory T-cell differentiation and inhibit T cell proliferation. These and other related data reinforce the notion that mechanical stimulation plays a crucial role in controlling inflammation and immunomodulation of periodontal tissues. Further investigations, however, are warranted to evaluate the immunomodulatory features of PDLSCs so as to understand the pathological basis of periodontal disease and translate these into clinical interventions.
Effect of circadian clock disruption on type 2 diabetes Peer-reviewed

Tran H, Kondo T, Ashry A, Fu Y, Okawa H, Sawangmake C, Egusa H

Front Physiol　15　1435848　2024/08

DOI： 10.3389/fphys.2024.1435848 　
東北大学病院嚥下治療センター・歯科における筋萎縮性側索硬化症患者に対する介入の実態舌接触補助床の適用について Peer-reviewed

互野亮, 白石成, 千葉智弘, 砂田慶一, 小川徹, 江草宏, 小山重人

顎顔面補綴　47　(1)　14-20　2024/06
Publisher: (一社)日本顎顔面補綴学会
ISSN： 0389-4045

eISSN： 2435-0389
Effects of mechanical loading on matrix homeostasis and differentiation potential of periodontal ligament cells: A scoping review Peer-reviewed

Pakpahan ND, Kyawsoewin M., Manokawinchoke J., Termkwancharoen C., Egusa H., Limraksasin P., Osathanon T.

J Periodont Res　59　(5)　877-906　2024/05/12

DOI： 10.1111/jre.13284 　
Current status and future prospects of CAD/CAM resin crown treatment Invited Peer-reviewed

Hiroshi Egusa

The Journal of the Japanese Society for Dental Materials and Devices　43　(2)　71-74　2024/05
Zinc- and fluoride-releasing bioactive glass as a novel bone substitute Peer-reviewed

Kondo T., Otake K., Kakinuma H., Sato Y., Ambo S., Egusa H.

J Dent Res　103　(5)　526-535　2024/04/06

DOI： 10.1177/00220345241231772 　
Mechanism underlying ultraviolet-irradiation-induced discoloration of dental zirconia ceramics stabilized with 3 and 5 mol% yttria Peer-reviewed

Kurihara A., Nakamura K., Shishido S., Inagaki R., Harada A., Kanno T., Egusa H.

Ceram Int　50　(7)　12136-12145　2024/04
Publisher: Elsevier BV
DOI： 10.1016/j.ceramint.2024.01.117 　

ISSN： 0272-8842
In vitro evaluation of shape-memory hydrogels for removable dental prostheses and optimization of phase-transition temperature for intraoral use. International-journal

Hiroaki Kakinuma, Shigeto Koyama, Takeru Kondo, Takayuki Harata, Hidemitsu Furukawa, Hiroshi Egusa

The Journal of prosthetic dentistry　2024/02/20

DOI： 10.1016/j.prosdent.2024.01.016 　

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STATEMENT OF PROBLEM: Removable dental prostheses require periodic relining with the loss of intaglio surface fit because of mucosal shape changes over time. Therefore, a new material with high adaptability to tissue changes over time would be beneficial. PURPOSE: This study focused on a shape-memory gel (SMG) that softens when heated, retains its shape when cooled, and returns to its original shape when reheated. The purpose was to optimize SMG for intraoral use by controlling the ratio of 2 acrylate monomers and to evaluate the changes in the shape memory and physical properties of SMG with temperature and to evaluate biocompatibility. MATERIAL AND METHODS: SMG specimens were synthesized using the following mixing ratios of 2 monomers, docosyl acrylate (DA) and stearyl acrylate (SA): 0:100, 25:75, 50:50, 75:25, and 100:0. SMG specimens were photopolymerized using a fluorescent light-polymerizing unit. To evaluate shape memory as a function of temperature, permanent deformation was measured based on the standardized compression set test for thermoplastic rubber. For evaluation of the physical properties and cytotoxicity, a 3-dimensionally printed denture base material was used as the control material. All assessments were compared between the groups by using 1-way analysis of variance followed by the Tukey-Kramer multiple comparison test (α=.05). RESULTS: SMGs with a higher amount of DA maintained their compressed shape at room and intraoral temperatures. However, the SMG matrices softened and recovered their original shapes above 60 °C. SMGs showed Shore A hardness equivalent to that of the denture-base polymer material at intraoral temperatures because of the high phase-transition temperature. The low water solubility of SMGs supported the biocompatibility test results. CONCLUSIONS: SMG, in which the phase-transition temperature was controlled by mixing acrylate monomers with different melting points, exhibited shape memory in the intraoral environment. The results indicate the feasibility of applying SMG for the fabrication of removable dental prostheses because of its high adaptability to tissue changes over time and biocompatibility.
Innate immune regulation in dental implant osseointegration. Peer-reviewed

Kondo T., Yamada M., Egusa H.

J Prosthodont Res　68　(4)　511-521　2024/02/13

DOI： 10.2186/jpr.JPR_D_23_00198 　

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PURPOSE: Dental implant osseointegration comprises two types of bone formation-contact and distance osteogenesis-which result in bone formation originating from the implant surface or bone edges, respectively. The physicochemical properties of the implant surface regulate initial contact osteogenesis by directly tuning the osteoprogenitor cells in the peri-implant environment. However, whether these implant surface properties can regulate osteoprogenitor cells distant from the implant remains unclear. Innate immune cells, including neutrophils and macrophages, govern bone metabolism, suggesting their involvement in osseointegration and distance osteogenesis. This narrative review discusses the role of innate immunity in osseointegration and the effects of implant surface properties on distant osteogenesis, focusing on innate immune regulation. STUDY SELECTION: The role of innate immunity in bone formation and the effects of implant surface properties on innate immune function were reviewed based on clinical, animal, and in vitro studies. RESULTS: Neutrophils and macrophages are responsible for bone formation during osseointegration, via inflammatory mediators. The microroughness and hydrophilic status of titanium implants have the potential to alleviate this inflammatory response of neutrophils, and induce an anti-inflammatory response in macrophages, to tune both contact and distance osteogenesis through the activation of osteoblasts. Thus, the surface micro-roughness and hydrophilicity of implants can regulate the function of distant osteoprogenitor cells through innate immune cells. CONCLUSIONS: Surface modification of implants aimed at regulating innate immunity may be useful in promoting further osteogenesis and overcoming the limitations encountered in severe situations, such as early loading protocol application.
Visualization of droplets and aerosols in simulated dental treatments to clarify the effectiveness of oral suction devices Peer-reviewed

Jun Watanabe, Yoko Iwamatsu-kobayashi, Kenji Kikuchi, Tomonari Kajita, Hiromitsu Morishima, Kensuke Yamauchi, Wataru Yashiro, Hidekazu Nishimura, Hiroyasu Kanetaka, Hiroshi Egusa

J Prosthodont Res　68　(1)　85-91　2024
Publisher: Japan Prosthodontic Society
DOI： 10.2186/jpr.jpr_d_23_00013 　

ISSN： 1883-1958

eISSN： 1883-9207
Impact of implant location on load distribution of implant-assisted removable partial dentures: a review of in vitro model and finite-element analysis studies Peer-reviewed

Ichikawa H, Yoda N, Ogawa T, Iwamoto M, Kawata T, Egusa H, Sasaki K

Int J Implant Dent　9　(1)　31　2023/09
Roles of extracellular adenosine triphosphate on the functions of periodontal ligament cells Peer-reviewed

Kyawsoewin M, Manokawinchoke J, Namangkalakul W, Egusa H, Limraksasin P, Osathanon T

BDJ Open　9　(1)　28　2023/07
Stiffness-tunable hydrogel-sandwich culture modulates the YAP-mediated mechanoresponse in induced-pluripotent stem cell embryoid bodies and augments cardiomyocyte differentiation Peer-reviewed

Nattasit P, Niibe K, Yamada M, Ohori-Morita Y, Limraksasin P, Tiskratok W, Yamamoto M, Egusa H

Macromol Biosci　23　(7)　e2300021-e2300021　2023/07
Publisher: Wiley
DOI： 10.1002/mabi.202300021 　

ISSN： 1616-5187

eISSN： 1616-5195
Incomplete polymerization of dual-cured resin cement due to attenuated light through zirconia induces inflammatory responses International-journal Peer-reviewed

Kondo T, Kakinuma H, Fujimura K, Ambo S, Otake K, Sato Y, Egusa H

Int J Mol Sci　24　(12)　9861　2023/06

DOI： 10.3390/ijms24129861 　

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Zirconia restorations are becoming increasingly common. However, zirconia reduces the polymerization of dual-cured resin cement owing to light attenuation, resulting in residual resin monomers. This study investigated the effects of dual-cured resin cement, with incomplete polymerization owing to attenuated light through zirconia, on the inflammatory response in vitro. The dual-cured resin cement (SA Luting Multi, Kuraray) was light-irradiated through zirconia with three thickness diameters (1.0, 1.5, and 2.0 mm). The light transmittance and the degree of conversion (DC) of the resin cement significantly decreased with increasing zirconia thickness. The dual-cured resin cement in 1.5 mm and 2.0 mm zirconia and no-irradiation groups showed significantly higher amounts of hydroxyethylmethacrylate and triethyleneglycol dimethacrylate elution and upregulated gene expression of proinflammatory cytokines IL-1β and IL-6 from human gingival fibroblasts (hGFs) and TNFα from human monocytic cells, compared with that of the 0 mm group. Dual-cured resin cement with lower DC enhanced intracellular reactive oxygen species (ROS) levels and activated mitogen-activated protein (MAP) kinases in hGFs and monocytic cells. This study suggests that dual-cured resin cement with incomplete polymerization induces inflammatory responses in hGFs and monocytic cells by intracellular ROS generation and MAP kinase activation.
Mechanoregulation of Osteoclastogenesis-Inducing Potentials of Fibrosarcoma Cell Line by Substrate Stiffness Peer-reviewed

Watcharaphol Tiskratok, Masahiro Yamada, Jun Watanabe, Qu Pengyu, Tsuyoshi Kimura, Hiroshi Egusa

Int J Mol Sci　24　(10)　8959　2023/05/18

DOI： 10.3390/ijms24108959 　
A 19-year study of dental needlestick and sharps injuries in Japan Peer-reviewed

Iwamatsu-Kobayashi Y, Watanabe J, Kusama T, Endo H, Ikeda S, Tokuda K, Igarashi K, Egusa H

Int Dent J　73　(1)　114-120　2023/02

DOI： 10.1016/j.identj.2022.04.009 　

ISSN： 1875-595X 0020-6539
Beyond diversity in prosthodontic research Invited Peer-reviewed

Egusa H

J Prosthodont Res　67　(1)　vi-vii　2023/01

DOI： 10.2186/jpr.JPR_D_22_00298 　

ISSN： 1883-1958
Substrate stiffness controls proinflammatory responses in human gingival fibroblasts Peer-reviewed

Tiskratok W, Yamada M, Watanabe J, Kartikasari N, Kimura T, Egusa H

Sci Rep　13　(1)　1358　2023/01
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41598-023-28541-z 　

eISSN： 2045-2322

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Abstract Soft gingiva is often compromised in gingival health; however, the underlying biological mechanisms remain unknown. Extracellular matrix (ECM) stiffness is involved in the progression of various fibroblast-related inflammatory disorders via cellular mechanotransduction. Gingival stiffness might regulate cellular mechanotransduction-mediated proinflammatory responses in gingival fibroblasts. This in vitro study aims to investigate the effects of substrate stiffness on proinflammatory responses in human gingival fibroblasts (hGFs). The hGFs isolated from two healthy donors cultured on type I collagen-coated polydimethylsiloxane substrates with different stiffnesses, representing soft (5 kPa) or hard (25 kPa) gingiva. Expression levels of proinflammatory mediators, prostaglandin E2 or interleukin-1β, in hGFs were significantly higher with the soft substrate than with the hard substrate, even without and with lipopolysaccharide (LPS) to induce inflammation. Expression levels of gingival ECM and collagen cross-linking agents in hGFs were downregulated more with the soft substrate than with the hard substrate through 14 days of culture. The soft substrate suppressed the expression of mechanotransduction-related transcriptional factors and activated the expression of inflammation-related factors, whereas the hard substrate demonstrated the opposite effects. Soft substrate induced proinflammatory responses and inhibition of ECM synthesis in hGFs by inactivating cellular mechanotransduction. This supports the importance of ECM stiffness in gingival health.
Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cells Peer-reviewed

Manokawinchoke J, Chareonvit S, Trachoo V, Limraksasin P, Egusa H, Osathanon T

J Dent Sci　18　(1)　105-111　2023/01

DOI： 10.1016/j.jds.2022.07.003 　

ISSN： 2213-8862 1991-7902
Current perspectives of residual ridge resorption: Pathological activation of oral barrier osteoclasts Invited Peer-reviewed

Kondo T, Kanayama K, Egusa H, Nishimura I

J Prosthodont Res　67　(1)　12-22　2023/01

DOI： 10.2186/jpr.JPR_D_21_00333 　

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PURPOSE: Tooth extraction is a last resort treatment for resolving pathological complications of dentition induced by infection and injury. Although the extraction wound generally heals uneventfully, resulting in the formation of an edentulous residual ridge, some patients experience long-term and severe residual ridge reduction. The objective of this review was to provide a contemporary understanding of the molecular and cellular mechanisms that may potentially cause edentulous jawbone resorption. STUDY SELECTION: Clinical, in vivo, and in vitro studies related to the characterization of and cellular and molecular mechanisms leading to residual ridge resorption. RESULTS: The alveolar processes of the maxillary and mandibular bones uniquely juxtapose the gingival tissue. The gingival oral mucosa is an active barrier tissue that maintains homeostasis of the internal organs through its unique barrier immunity. Tooth extraction not only generates a bony socket but also injures oral barrier tissue. In response to wounding, the alveolar bone socket initiates regeneration and remodeling through coupled bone formation and osteoclastic resorption. Osteoclasts are also found on the external surface of the alveolar bone, interfacing the oral barrier tissue. Osteoclasts in the oral barrier region are not coupled with osteoblastic bone formation and often remain active long after the completion of wound healing, leading to a net decrease in the alveolar bone structure. CONCLUSION: The novel concept of oral barrier osteoclasts may provide important clues for future clinical strategies to maintain residual ridges for successful prosthodontic and restorative therapies.
Cyclic pressure-induced cytokines from gingival fibroblasts stimulate osteoclast activity: Clinical implications for alveolar bone loss in denture wearers Peer-reviewed

Akashi Y, Nagasaki A, Okawa H, Matsumoto T, Kondo T, Yatani H, Nishimura I, Egusa H

J Prosthodont Res　67　(1)　77-86　2023

DOI： 10.2186/jpr.JPR_D_21_00238 　

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PURPOSE: The involvement of oral mucosa cells in mechanical stress-induced bone resorption is unclear. The aim of this study was to investigate the effects of cyclic pressure-induced cytokines from oral mucosal cells (human gingival fibroblasts: hGFs) on osteoclast activity in vitro. METHODS: Cyclic pressure at 50 kPa, which represents high physiologic occlusal force of dentures on the molar area, was applied to hGFs. NFAT-reporter stable RAW264.7 preosteoclasts (NFAT/Luc-RAW cells) were cultured in conditioned medium collected from hGF cultures under cyclic pressure or static conditions. NFAT activity and osteoclast formation were determined by luciferase reporter assay and TRAP staining, respectively. Cyclic pressure-induced cytokines in hGF culture were detected by ELISA, real-time RT-PCR, and cytokine array analyses. RESULTS: Conditioned media from hGFs treated with 48 hours of cyclic pressure significantly induced NFAT activity and increased multinucleated osteoclast formation. Furthermore, the cyclic pressure significantly increased the bone resorption activity of RAW264.7 cells. Cyclic pressure significantly increased the expression of major inflammatory cytokines including IL-1β/IL-1β, IL-6/IL-6, IL-8/IL-8 and MCP-1/CCL2 in hGFs compared to hGFs cultured under static conditions, and it suppressed osteoprotegerin (OPG/OPG) expression. A cytokine array detected 12 cyclic pressure-induced candidates. Among them, IL-8, decorin, MCP-1 and ferritin increased, whereas IL-28A and PDGF-BB decreased, NFAT activation of NFAT/Luc-RAW cells. CONCLUSION: These results suggest that cyclic pressure-induced cytokines from hGFs promote osteoclastogenesis, possibly including up-regulation of IL-1β, IL-6, IL-8 and MCP-1, and down-regulation of OPG. These findings introduce the possible involvement of GFs in mechanical stress-induced alveolar ridge resorption, such as in denture wearers.
In vivo measurement of three-dimensional load exerted on dental implants: a literature review Peer-reviewed

Assoratgoon I, Yoda N, Iwamoto M, Sato T, Kawata T, Egusa H, Sasaki K

Int J Implant Dent　8　(1)　52　2022/11
Application of shear stress for enhanced osteogenic differentiation of mouse induced pluripotent stem cells Peer-reviewed

Limraksasin P, Nattasit P, Manokawinchoke J, Tiskratok W, Vinaikosol N, Okawa H, Limjeerajarus CN, Limjeerajarus N, Pavasant P, Osathanon T, Egusa H

Sci Rep　12　(1)　19021　2022/11

DOI： 10.1038/s41598-022-21479-8 　

ISSN： 2045-2322
Comparison of the accuracy of resin-composite crowns fabricated by three-dimensional printing and milling methods Peer-reviewed

Kakinuma H, Izumita K, Yoda N, Egusa H, Sasaki K

Dent Mater J　41　(6)　808-815　2022/11

DOI： 10.4012/dmj.2022-074 　

ISSN： 1881-1361 0287-4547
Titanium nanotopography induces osteocyte lacunar-canalicular networks to strengthen osseointegration Peer-reviewed

He X, Yamada M, Watanabe J, Tiskratok W, Ishibashi M, Kitaura H, Mizoguchi I, Egusa H

Acta Biomater　151　613-627　2022/10
Publisher: Elsevier BV
DOI： 10.1016/j.actbio.2022.08.023 　

ISSN： 1742-7061
COVID-19-Related Symptoms during the SARS-CoV-2 Omicron (B.1.1.529) Variant Surge in Japan. Peer-reviewed

Akaishi T, Kushimoto S, Katori Y, Sugawara N, Egusa H, Igarashi K, Fujita M, Kure S, Takayama S, Abe M, Kikuchi A, Ohsawa M, Ishizawa K, Abe Y, Imai H, Inaba Y, Iwamatsu-Kobayashi Y, Nishioka T, Onodera K, Ishii T

Tohoku J Exp Med　258　(2)　103-110　2022/09

DOI： 10.1620/tjem.2022.J067 　

ISSN： 1349-3329 0040-8727
Correction of large jawbone defect in the mouse using immature osteoblast-like cells and a three dimensional polylactic acid scaffold Peer-reviewed

Suzuki S, Venkataiah VS, Yahata Y, Kitagawa A, Inagaki M, Njuguna MM, Nozawa R, Kakiuchi Y, Nakano M, Handa K, Yamada M, Egusa H, Saito M

PNAS Nexus　1　(4)　pgac151　2022/08
Effectiveness of third vaccine dose for coronavirus disease 2019 during the Omicron variant pandemic: a prospective observational study in Japan. Peer-reviewed

Akaishi T, Kushimoto S, Katori Y, Sugawara N, Egusa H, Igarashi K, Fujita M, Kure S, Takayama S, Abe M, Kikuchi A, Ohsawa M, Ishizawa K, Abe Y, Imai H, Inaba Y, Iwamatsu-Kobayashi Y, Nishioka T, Onodera K, Ishii T

Sci Rep　12　(1)　13589　2022/08

DOI： 10.1038/s41598-022-17990-7 　

ISSN： 2045-2322
Beyond the diversity in prosthodontic research Invited

Egusa H

Ann Jpn;Prosthodont Soc　14　227-228　2022/07
Leadership for the further growth of JPR Invited Peer-reviewed

Egusa H

J Prosthodont Res　66　(3)　vi-vii　2022/07

DOI： 10.2186/jpr.JPR_66_3_ed 　

ISSN： 1883-1958
Rapid and efficient generation of cartilage pellets from mouse induced pluripotent stem cells by transcriptional activation of BMP-4 with shaking culture

Zhang M, Niibe K, Kondo T, Limraksasin P, Okawa H, Miao X, Kamano Y, Yamada M, Jiang X, Egusa H

J Tissue Eng　13　20417314221114616　2022/07

DOI： 10.1177/20417314221114616 　

ISSN： 2041-7314
Titania nanospikes activate macrophage phagocytosis by ligand-independent contact stimulation Peer-reviewed

Kartikasari N, Yamada M, Watanabe J, Tiskratok W, He X, Egusa H

Sci Rep　: 12　(1)　12250　2022/07
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41598-022-16214-2 　

eISSN： 2045-2322

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Abstract Macrophage phagocytosis is an important research target to combat various inflammatory or autoimmune diseases; however, the phenomenon has never been controlled by artificial means. Titania nanospikes created by alkaline etching treatment can tune macrophage polarization toward a M1-like type and might regulate macrophage phagocytosis. This in vitro study aimed to determine whether the two-dimensional titania nanosurfaces created by alkaline etching treatment activated the macrophage phagocytosis by nanospike-mediated contact stimulation. On two-dimensional pure titanium sheets, alkaline etching treatments with different protocols created superhydrophilic nanosurfaces with hydroxyl function groups and moderate or dense nanospikes. Both types of titania nanosurfaces promoted the phagocytic activity of the mouse macrophage-like cell line, J774A.1, through upregulation of M1 polarization markers and phagocytosis-related receptors, such as toll-like receptors (TLR2 and 4). In contrast, the hydrophobic smooth or micro-roughened titanium surfaces did not activate macrophage phagocytosis or the expression of related receptors. These phenomena remained unchanged even under the antibody blockade of macrophage TLR2 but were either suppressed or augmented for each surface excited by ultraviolet irradiation. Titania nanospikes induced paxillin expression and provided physical stimuli to macrophages, the extent of which was positively correlated with TLR expression levels. Ligand stimulation with lipopolysaccharide did not upregulate macrophage TLR expression but further enhanced M1 marker expression by titania nanosurfaces. These results showed that the two-dimensional titania nanosurfaces activated macrophage phagocytosis by enhancing expression of phagocytosis-related receptors through nanospike-mediated contact stimulation, in assistance with physical surface properties, in a ligand-independent manner.
Epiprofin transcriptional activation promotes ameloblast induction from mouse induced pluripotent stem cells via the BMP-Smad signaling axis Peer-reviewed

Miao X, Niibe K, Fu Y, Zhang M, Nattasit P, Ohori-Morita Y, Nakamura T, Jiang X, Egusa H

Front Bioeng Biotechnol　10　Article 890882　2022/06
Publisher: Frontiers Media SA
DOI： 10.3389/fbioe.2022.890882 　

eISSN： 2296-4185

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The transcriptional regulation of induced pluripotent stem cells (iPSCs) holds promise for their directed differentiation into ameloblasts, which are usually lost after tooth eruption. Ameloblast differentiation is regulated by multiple signaling molecules, including bone morphogenetic proteins (BMPs). Epiprofin (Epfn), a transcription factor, is expressed in the dental epithelium, and epithelial Epfn overexpression results in ectopic ameloblast differentiation and enamel formation in mouse incisor, a striking phenotype resembling that of mice with deletion of follistatin (a BMP inhibitor). However, it remains unknown whether and how Epfn transcriptional activation promotes ameloblast induction from mouse iPSCs. Here, we generated doxycycline-inducible Epfn-expressing mouse iPSCs (Epfn-iPSCs). Ameloblasts, which are characterized by positive staining for keratin 14 and amelogenin and alizarin red S staining, were successfully derived from Epfn-iPSCs based on a stage-specific induction protocol, which involved the induction of the surface ectoderm, dental epithelial cells, and ameloblasts at stages 1, 2, and 3, respectively. Epfn activation by doxycycline at stages 2 and/or 3 decreased cell proliferation and promoted ameloblast differentiation, along with the upregulation of p-Smad1/5/8, a key regulator of the BMP-Smad signaling pathway. Gene analysis of the BMP-Smad signaling pathway-associated molecules revealed that Epfn activation decreased follistatin expression at stage 2, but increased BMP2/4/7 expression at stage 3. Perturbations in the ameloblast differentiation process were observed when the BMP-Smad signaling pathway was inhibited by a BMP receptor inhibitor (LDN-193189). Simultaneous LDN-193189 treatment and Epfn activation largely reversed the perturbations in ameloblast induction, with partial recovery of p-Smad1/5/8 expression, suggesting that Epfn activation promotes ameloblast induction from mouse iPSCs partially by upregulating BMP-Smad activity. These results reveal the potential regulatory networks between Epfn and the BMP-Smad pathway and suggest that Epfn is a promising target for inducing the differentiation of ameloblasts, which can be used in enamel and tooth regeneration.
The titanium nanosurface with biomimetic physical microenvironment to induce endogenous regeneration of the periodontium International-journal Peer-reviewed

Yamada M, Kimura T, Nakamura N, Watanabe J, Kartikasari N, He X, Tiskratok W, Yoshioka H, Shinno H, Egusa H

ACS Appl Mater Interfaces　14　(24)　27703-27719　2022/06

DOI： 10.1021/acsami.2c06679 　

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The periodontium supports the teeth by dentoalveolar fibrous joints that serve unique oral functions. Endogenous regeneration of the periodontium around artificial teeth (dental implants) provides a cost-effective solution for the extension of healthy life expectancy but remains a challenge in regenerative medicine. Biomimetics can create smart biomaterials that tune endogenous cells at a tissue-material interface. Here, we created a smart titanium nanosurface mimicking the surface nanotopography and micromechanical properties of the tooth root cementum (TRC), which is essential for the induction of dentoalveolar fibrous joints to regenerate the periodontium. After transplantation into the rat renal capsule, only the titanium artificial tooth with the TRC-mimetic nanosurface formed a complex dentoalveolar fibrous joint structure, with bone tissue, periodontal ligament (PDL), and TRC, in the decellularized jawbone matrix. TRC-mimetic titanium implants induce the formation of functional periodontium, even in a jawbone implantation model, which generally causes osseointegration (ankyloses). In human PDL cells, TRC analogousness in the surface mechanical microenvironment regulates matrix mineralization through bone sialoprotein expression and phosphorus metabolism, which are critical for cementogenesis. Therefore, the titanium nanosurfaces with nanotopographical and mechanical microenvironments mimicking the TRC surface induce dentoalveolar fibrous joints for periodontal regeneration by interfacial tuning of endogenous cells.
Potential complications of CAD/CAM-produced resin composite crowns on molars: A retrospective cohort study over four years International-journal Peer-reviewed

Inomata M, Harada A, Kasahara S, Kusama T, Ozaki A, Katsuda Y, Egusa H

PLoS One　17　(4)　e0266358　2022/04

DOI： 10.1371/journal.pone.0266358 　

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PURPOSE: Evaluation of the clinical performance of computer-aided design/computer-aided manufacturing-produced resin composite crowns (CAD/CAM composite crowns) on molars with a particular focus on placement location. METHODS: A retrospective cohort study was performed based on the clinical records of patients with CAD/CAM composite crowns on molars (June 2016 to March 2021). The hazard ratios (HRs) and 95% confidence intervals (95% CIs) were estimated based the Cox proportional hazard model to evaluate the effect of tooth location on complication type and occurrence. Covariates included crown location (maxilla/mandible, distalmost tooth/not distalmost tooth, and first molar/second or third molar) and endodontically treated (nonvital) or untreated (vital) tooth. RESULTS: Overall, 362 crowns were evaluated (mean follow-up: 378 days, median: 286 days), and 106 crowns (29.3%) showed complications, most frequently crown debonding. The cumulative success and survival rates were 70.9% and 93.7%, respectively, after 1 year and 49.5% and 86.5%, respectively, after 3 years. There was no significant difference in the HRs and log-rank tests in the Kaplan-Meier curves based on crown location parameters (P > 0.05). However, placement on vital teeth was associated with higher risks than on nonvital teeth (HR, 1.55; 95% CI, 1.03-2.23). In addition, the cement as a covariate yielded a high HR. CONCLUSIONS: The location of CAD/CAM composite molar crowns is unlikely a risk factor for complications; therefore, these crowns can be clinically applied to all molars. However, the application of such molar crowns to vital teeth and the use of a cement other than adhesive resin cement present risks.
Novel mesenchymal stem cell spheroids with enhanced stem cell characteristics and bone regeneration ability Peer-reviewed

Ohori-Morita Y, Niibe K, Limraksasin P, Nattasit P, Miao X, Yamada M, Mabuchi Y, Matsuzaki Y, Egusa H

Stem Cells Transl Med　11　(4)　434-449　2022/04

DOI： 10.1093/stcltm/szab030 　

ISSN： 2157-6580 2157-6564
Involvement of an FTO gene polymorphism in the temporomandibular joint osteoarthritis Peer-reviewed

Takaoka R, Kuyama K, Yatani H, Ishigaki S, Kayashima H, Koishi Y, Kato T, Egusa H, Uchiyama Y, Nakatani A, Shimamoto H

Clin Oral Investig　26　(3)　2965-2973　2022/03
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s00784-021-04278-9 　

ISSN： 1432-6981

eISSN： 1436-3771

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<title>Abstract</title><sec> <title>Objectives</title> The FTO gene has been reported as an obesity-associated gene and is also considered a risk gene for osteoarthritis (OA). However, its exact function is unclear, and there is conflicting evidence on the involvement of FTO polymorphisms in OA via obesity. The purpose of this study was to determine the effects of FTO polymorphism rs8044769 alleles on OA in the temporomandibular joint (TMJ), which is minimally affected by body weight. </sec><sec> <title>Materials and methods</title> A total of 324 TMJs (113 with OA and 211 without OA, serving as controls) from 162 Japanese patients with temporomandibular disorders and undergoing MRI examination were analyzed. Genotyping was conducted, and multivariate analysis was performed after adjusting for the effects of age, sex, body mass index, and TMJ disc abnormalities. </sec><sec> <title>Results</title> Mean age, BMI, and sex did not differ between the TMJs with OA and the TMJs without OA, but a significant difference was found for positional and dynamic disc abnormalities (<italic>P</italic> < 0.05). The allele frequency of FTO polymorphisms also differed significantly between the TMJs with OA and the TMJs without OA (<italic>P</italic> = 0.011). Moreover, logistic regression analysis showed no significant association between BMI (<italic>P</italic> = 0.581) and the occurrence of TMJOA but also indicated that the CC allele of rs8044769 is a risk factor for TMJOA (<italic>P</italic> = 0.040). </sec><sec> <title>Conclusions</title> Our results show that rs8044769 in the FTO gene might be involved in TMJOA. </sec><sec> <title>Clinical relevance</title> The present study provides a basis for a deeper understanding of the mechanism underlying degenerative skeletal diseases and the more effective selection and development of treatment strategies based on the patients’ genetic characteristics. </sec>
Cell-based double-screening method to identify a reliable candidate for osteogenesis-targeting compounds Peer-reviewed

Fukuyasu S, Kayashima H, Moribayashi A, Matsuoka S, Nagasaki A, Okawa H, Yatani H, Saeki M, Egusa H

Biomedicines　10　(2)　426-426　2022/02
Publisher: MDPI AG
DOI： 10.3390/biomedicines10020426 　

eISSN： 2227-9059

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Small-molecule compounds strongly affecting osteogenesis can form the basis of effective therapeutic strategies in bone regenerative medicine. A cell-based high-throughput screening system might be a powerful tool for identifying osteoblast-targeting candidates; however, this approach is generally limited with using only one molecule as a cell-based sensor that does not always reflect the activation of the osteogenic phenotype. In the present study, we used the MC3T3-E1 cell line stably transfected with the green fluorescent protein (GFP) reporter gene driven by a fragment of type I collagen promoter (Col-1a1GFP-MC3T3-E1) to evaluate a double-screening system to identify osteogenic inducible compounds using a combination of a cell-based reporter assay and detection of alkaline phosphatase (ALP) activity. Col-1a1GFP-MC3T3-E1 cells were cultured in an osteogenic induction medium after library screening of 1280 pharmacologically active compounds (Lopack1280). After 7 days, GFP fluorescence was measured using a microplate reader. After 14 days of osteogenic induction, the cells were stained with ALP. Library screening using the Col-1a1/GFP reporter and ALP staining assay detected three candidates with significant osteogenic induction ability. Furthermore, leflunomide, one of the three detected candidates, significantly promoted new bone formation in vivo. Therefore, this double-screening method could identify candidates for osteogenesis-targeting compounds more reliably than conventional methods.
Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC) Peer-reviewed

Fakhruddin KS, Samaranayake LP, Hamoudi RA, Ngo HC, Egusa H

J Oral Microbiol　14　(1)　2037832　2022/02

DOI： 10.1080/20002297.2022.2037832 　

ISSN： 2000-2297
JPR starts 2022 with a new design -Towards a new frontier in prosthodontics Invited Peer-reviewed

Egusa H

J Prosthodont Res　66　(1)　vi-vii　2022/01

DOI： 10.2186/jpr.JPR_66_1_ed 　

ISSN： 1883-1958
Intermittent compressive force induces cell cycling and reduces apoptosis in embryoid bodies of mouse induced pluripotent stem cells Peer-reviewed

Manokawinchoke J, Limraksasin P, Okawa H, Pavasant P, Egusa H, Osathanon T

Int J Oral Sci　14　(1)　1　2022/01

DOI： 10.1038/s41368-021-00151-3 　

ISSN： 2049-3169 1674-2818
Titanium surface with nanospikes tunes macrophage polarization to produce inhibitory factors for osteoclastogenesis through nanotopographic cues International-journal Peer-reviewed

Kartikasari N, Yamada M, Watanabe J, Tiskratok W, He X, Kamano Y, Egusa H

Acta Biomater　137　316-330　2022/01

DOI： 10.1016/j.actbio.2021.10.019 　

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Definitive prevention of inflammatory osteolysis around peri-implant bone tissue remains unestablished. M1 macrophages play a key role in the host defense against inflammatory osteolysis, and their polarization depends on cell shape. Macrophage polarization is controlled by environmental stimuli, particularly physicochemical cues and hence titanium nanosurface might tune macrophage polarization and function. This study determined whether titanium nanosurfaces with anisotropically patterned nanospikes regulates macrophage polarization for inhibiting osteoclast differentiation of osteoclast precursors. Alkaline-etching treatment with different protocols created two types of titanium nanosurfaces that had anisotropically patterned nanospikes with high or low distribution density, together with superhydrophilicity and the presence of hydroxyl groups. J774A.1 cells (mouse macrophage-like cell line), cultured on both titanium nanosurfaces, exhibited truly circulated shapes and highly expressed M1, but less M2, markers, without loss of viability. M1-like polarization of macrophages on both titanium nanosurfaces was independent of protein-mediated ligand stimulation or titanium surface hydrophilic or chemical status. In contrast, other smooth or micro-roughened titanium surfaces with little or no nanospikes did not activate macrophages under any culture conditions. Macrophage culture supernatants on both titanium nanosurfaces inhibited osteoclast differentiation of RAW264.7 cells (mouse osteoclast precursor cell line), even when co-incubated with osteoclast differentiation factors. The inhibitory effects on osteoclast differentiation tended to be higher in macrophages cultured on titanium nanosurfaces with denser nanospikes. These results showed that titanium nanosurfaces with anisotropically patterned nanospikes tune macrophage polarization for inhibiting osteoclast differentiation of osteoclast precursors, with nanotopographic cues rather than other physicochemical properties. STATEMENT OF SIGNIFICANCE: Peri-implant inflammatory osteolysis is one of the serious issues for dental and orthopedic implants. Macrophage polarization and function are key for prevention of peri-implant inflammatory osteolysis. Macrophage polarization can be regulated by the biomaterial's surface physicochemical properties such as hydrophilicity or topography. However, there was no titanium surface modification to prevent inflammatory osteolysis through immunomodulation. The present study showed for the first time that the titanium nanosurfaces with anisotropically patterned nanospikes, created by the simple alkali-etching treatment polarized macrophages into M1-like type producing the inhibitory factor on osteoclast differentiation. This phenomenon attributed to nanotopographic cues, but not hydrophilicity on the titanium nanosurfaces. This nanotechnology might pave the way to develop the smart implant surface preventing peri-implant inflammatory osteolysis through immunomodulation.
A method for in vitro fabrication of hybrid bone/cartilage tissue using mouse induced pluripotent stem cells Invited Peer-reviewed

Limraksasin P, Egusa H

Methods Mol Biol　2454　509-520　2022
Publisher: Springer US
DOI： 10.1007/7651_2021_361 　

ISSN： 1064-3745

eISSN： 1940-6029
Impact of adhesive primer and light-curing on polymerization kinetics of self-adhesive resin cement in association with free radical reaction Peer-reviewed

Ozaki A, Shishido S, Nakamura K, Harada A, Katsuda Y, Kanno T, Egusa H

Eur J Oral Sci　129　(6)　e12828　2021/12
Publisher: Wiley
DOI： 10.1111/eos.12828 　

ISSN： 0909-8836

eISSN： 1600-0722
JPR continues to grow as a leading dental journal and international public asset Invited Peer-reviewed

Egusa H

J Prosthodont Res　65　(3)　vi-vi　2021/08
Publisher: Japan Prosthodontic Society
DOI： 10.2186/jpr.jpr_65_3_ed 　

ISSN： 1883-1958

eISSN： 1883-9207
Profuse diversity and acidogenicity of the candida-biome of deep carious lesions of severe early childhood caries (S-ECC) Peer-reviewed

Fakhruddin KS, Samaranayake LP, Egusa H, Ngo HC, Pesee S

J Oral Microbiol　13　(1)　1964277　2021/08
Publisher: Informa {UK} Limited
DOI： 10.1080/20002297.2021.1964277 　

ISSN： 2000-2297
Stage-specific role of amelx activation in stepwise ameloblast induction from mouse induced pluripotent stem cells Peer-reviewed

Miao X, Niibe K, Zhang M, Liu Z, Nattasit P, Ohori-Morita Y, Nakamura T, Jiang X, Egusa H

Int J Mol Sci　22　(13)　7195　2021/07

DOI： 10.3390/ijms22137195 　

ISSN： 1661-6596

eISSN： 1422-0067
Deficiency of Lipin2 Results in Enhanced NF-κB Signaling and Osteoclast Formation in RAW-D Murine Macrophages

Asami Watahiki, Seira Hoshikawa, Mitsuki Chiba, Hiroshi Egusa, Satoshi Fukumoto, Hiroyuki Inuzuka

International Journal of Molecular Sciences　2021/03

DOI： 10.3390/ijms22062893 　
Deficiency of lipin2 results in enhanced proinflammatory response and osteoclast formation in macrophages Peer-reviewed

Watahiki A, Hoshikawa S, Chiba M, Egusa H, Fukumoto S, Inuzuka H

Int J Mol Sci　22　(6)　2893　2021/03
Mechanical loading and the control of stem cell behavior Peer-reviewed

Manokawinchoke J, Pavasant P, Limjeerajarus CN, Limjeerajarus N, Osathanon T, Egusa H

Arch Oral Biol　125　105092-105092　2021/02
Publisher: Elsevier {BV}
DOI： 10.1016/j.archoralbio.2021.105092 　

ISSN： 0003-9969
Notch signaling in induced pluripotent stem cells

Osathanon, T., Egusa, H.

Molecular Players in iPSC Technology　2021

DOI： 10.1016/B978-0-323-90059-1.00003-8 　
Deficiency of lipin2 results in enhanced nf‐κb signaling and osteoclast formation in raw‐d murine macrophages

Watahiki, A., Hoshikawa, S., Chiba, M., Egusa, H., Fukumoto, S., Inuzuka, H.

International Journal of Molecular Sciences　22　(6)　2021

DOI： 10.3390/ijms22062893 　

ISSN： 1422-0067 1661-6596
Clinical evaluation of monolithic zirconia crowns: A failure analysis of clinically obtained cases from a 3.5-year study Peer-reviewed

Miura S, Yamauchi S, Kasahara S, Katsuda Y, Fujisawa M, Egusa H

J Prosthodont Res　65　(2)　148-154　2021/01

DOI： 10.2186/jpr.JPOR_2019_643 　

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PURPOSE: The primary purpose of this study was to examine the clinical performance of monolithic zirconia single crowns in terms of short-term failure or complications. The secondary purpose was to detect the originating flaws of clinically failed monolithic zirconia crowns to find the causes of failure. METHODS: A short-term prospective cohort study based on record evaluation and clinical examination of patients treated with tooth-supported monolithic zirconia crowns was performed in the Department of Fixed Prosthodontics, Tohoku University Hospital, Japan. The crowns were prepared during the follow-up period from April 2014 to July 2018. The 3.5-year cumulative success and survival rates were set as primary endpoints. Fractures of the crown or fragments were inspected under a scanning electron microscope for descriptive fractography. RESULTS: During the study period, 40 monolithic zirconia crowns were placed. Four crowns experienced clinical complications, including: 1) fracture of the crown (two crowns), 2) abrasion of the crown (one crown), and 3) fracture of the antagonist tooth (one crown). The estimated Kaplan-Meier 3.5-year success and survival rates were 90.5% (95% confi dence interval [CI]: 73.1-97.1) and 92.8% (95% CI: 74.1-98.3), respectively. Fractography revealed that all fractures were initiated from the wear phase on the occlusal surface. CONCLUSIONS: The results of this study suggest that the molar application of monolithic zirconia crowns requires detailed attention to interocclusal clearance and the restoration of the antagonist tooth.
Guidelines for the innovation of dental education under the coronavirus disease 2019 pandemic situation Peer-reviewed

Hong G, Chang TY, Terry A, Chuenjitwongsa S, Park YS, Tsoi KH, Sun JX, Kusdhany S, Egusa H, Yamada S, Kwon JS, Seow LL, Garcia CG, Loke WM, Auychai P, Hsu ML

J Oral Sci　63　(1)　107-110　2020/12

DOI： 10.2334/josnusd.20-0399 　

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During the current coronavirus disease 2019 (COVID-19) pandemic, dental education and training requiring face-to-face interaction must prioritize infection prevention and the safety of students, staff, and patients. In July 2020, the Association for Dental Education, Asia Pacific (ADEAP) published safety guidelines for safe dental education during the COVID-19 pandemic. These guidelines summarize ADEAP recommendations for classroom-based courses, reopening of simulated training courses and dental clinics, and provision of clinical skills training courses in dentistry during the COIVID-19 pandemic. They have been formulated to ensure the safety of students and teaching staff, dental auxiliary staff, and patients. However, the present guidelines are considered appropriate only when the number of COVID-19 epidemic cases has been significantly reduced, i.e. when the epidemiological curve has flattened in the area concerned. The criteria for lifting restrictions on activities should be consistent with relevant jurisdictional guidelines.
Gene transfection achieved by utilizing antibacterial calcium phosphate nanoparticles for enhanced regenerative therapy Peer-reviewed

Chen Xiang, Taichi Tenkumo, Toru Ogawa, Yoshiaki K, a, Keisuke Nakamura, Midori Shirato, Viktoriya Sokolova, Matthias Epple, Yuya Kamano, Hiroshi Egusa, Keiichi Sasaki

Acta Biomater　119　375-389　2020/11
Publisher: Elsevier {BV}
DOI： 10.1016/j.actbio.2020.11.003 　

ISSN： 1742-7061
A shaking-culture method for generating bone marrow derived mesenchymal stromal/stem cell-spheroids with enhanced multipotency in vitro International-journal Peer-reviewed

Niibe K, Ohori-Morita Y, Zhang M, Mabuchi Y, Matsuzaki Y, Egusa H

Front Bioeng Biotechnol　10　(1)　14996-590332　2020/09

DOI： 10.3389/fbioe.2020.590332 　

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Mesenchymal stromal/stem cells (MSCs), which generally expand into adherent monolayers, readily lose their proliferative and multilineage potential following repeated passages. Floating culture systems can be used to generate MSC spheroids, which are expected to overcome limitations associated with conventional adherent cultures while facilitating scaffold-free cell transplantation. However, the phenotypic characteristics of spheroids after long-term culture are unknown. In addition, regenerative therapies require new culture systems to maintain their undifferentiated state. In this study, we established a novel culture method employing three-dimensional (3D) "shaking" to generate MSC spheroids using bone marrow derived MSCs. Floating 3D cultures of mouse or human MSCs formed spheroids after shaking (85-95 rpm), within 1 month. These spheroids maintained their osteogenic-, adipogenic-, and chondrogenic-differentiation capacity. The adipogenic-differentiation capacity of adherent cultured mouse and human MSCs, which is lost following several passages, was remarkedly restored by shaking-culture. Notably, human MSC spheroids exhibited a renewable "undifferentiated MSC-pool" property, wherein undifferentiated MSCs grew from spheroids seeded repeatedly on a plastic culture dish. These data suggest that the shaking-culture method maintains and restores multipotency that is lost following monolayer expansion and thereby shows potential as a promising strategy for regenerative therapies with mesenchymal tissues.
Glass-fiber reinforced composite resin fixed dental prostheses : a case series study Peer-reviewed

Harada A, Egusa H

Tohoku Univ Dent J　37/38/39　17-25　2020/09
Shaking culture enhances chondrogenic differentiation of mouse induced pluripotent stem cell constructs International-journal Peer-reviewed

Limraksasin P, Kosaka Y, Zhang M, Horie N, Kondo T, Okawa H, Yamada M, Egusa H

Sci Rep　10　(1)　14996-14996　2020/09

DOI： 10.1038/s41598-020-72038-y 　

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Mechanical loading on articular cartilage induces various mechanical stresses and strains. In vitro hydrodynamic forces such as compression, shear and tension impact various cellular properties including chondrogenic differentiation, leading us to hypothesize that shaking culture might affect the chondrogenic induction of induced pluripotent stem cell (iPSC) constructs. Three-dimensional mouse iPSC constructs were fabricated in a day using U-bottom 96-well plates, and were subjected to preliminary chondrogenic induction for 3 days in static condition, followed by chondrogenic induction culture using a see-saw shaker for 17 days. After 21 days, chondrogenically induced iPSC (CI-iPSC) constructs contained chondrocyte-like cells with abundant ECM components. Shaking culture significantly promoted cell aggregation, and induced significantly higher expression of chondrogenic-related marker genes than static culture at day 21. Immunohistochemical analysis also revealed higher chondrogenic protein expression. Furthemore, in the shaking groups, CI-iPSCs showed upregulation of TGF-β and Wnt signaling-related genes, which are known to play an important role in regulating cartilage development. These results suggest that shaking culture activates TGF-β expression and Wnt signaling to promote chondrogenic differentiation in mouse iPSCs in vitro. Shaking culture, a simple and convenient approach, could provide a promising strategy for iPSC-based cartilage bioengineering for study of disease mechanisms and new therapies.
Recapitulation of cartilage/bone formation using iPSCs via biomimetic 3D rotary culture approach for developmental engineering Peer-reviewed

Zhang M, Shi J, Xie M, Wen J, Niibe K, Zhang X, Luo J, Yan R, Zhang Z, Egusa H, Jiang X

Biomaterials　260　120334-120334　2020/08
Publisher: Elsevier BV
DOI： 10.1016/j.biomaterials.2020.120334 　

ISSN： 0142-9612
NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells Peer-reviewed

Manokawinchoke J, Sumrejkanchanakij P, Boonprakong L, Pavasant P, Egusa H, Osathanon T

Sci Rep　10　(1)　13329　2020/08
Publisher: Springer Science and Business Media {LLC}
DOI： 10.1038/s41598-020-70277-7 　

ISSN： 2045-2322
Mechanical and microstructural properties of ultra-translucent dental zirconia ceramic stabilized with 5 mol% yttria Peer-reviewed

Harada A, Shishido S, Barkarmo S, Inagaki R, Kanno T, Örtengren U, Egusa H, Nakamura K

J Mech Behav Biomed Mater　111　103974-103974　2020/07
Publisher: Elsevier {BV}
DOI： 10.1016/j.jmbbm.2020.103974 　

ISSN： 1751-6161
Clinical efficacy and the antimicrobial potential of silver formulations in arresting dental caries: a systematic review Peer-reviewed

Kausar Sadia Fakhruddin, Hiroshi Egusa, Hien Chi Ngo, Chamila P, uwawala, Siripen Pesee, Lakshman Perera Samaranayake

BMC Oral Health　20　(1)　160　2020/06
Publisher: Springer Science and Business Media {LLC}
DOI： 10.1186/s12903-020-01133-3 　

ISSN： 1472-6831
Investigate the odontogenic differentiation and dentin-pulp tissue regeneration potential of neural crest cells Peer-reviewed

Zhang M, Zhang X, Luo J, Yan R, Niibe K, Egusa H, Zhang Z, Xie M, Jiang X

Front Bioeng Biotechnol　8　475　2020/06
Publisher: Frontiers Media SA
DOI： 10.3389/fbioe.2020.00475 　

eISSN： 2296-4185
Size-optimized micro-space culture facilitates differentiation of mouse induced pluripotent stem cells into osteoid-rich bone constructs Peer-reviewed

Limraksasin P, Okawa H, Zhang M, Kondo T, Osathanon T, Pavasant P, Egusa H

Stem Cells Int　2020　7082679-14　2020/05
Publisher: Hindawi Limited
DOI： 10.1155/2020/7082679 　

ISSN： 1687-966X

eISSN： 1687-9678

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Microspace culture is promising for self-organization of induced pluripotent stem cells (iPSCs). However, the optimal size of microspaces for osteogenic differentiation is unclear. We hypothesized that a specific microspace size could facilitate self-organizing iPSC differentiation to form bone-like tissue <italic>in vitro</italic>. The objectives of this study were to investigate such effects of microspace size and to evaluate bone regeneration upon transplantation of the resulting osteogenic constructs. Dissociated mouse gingival fibroblast-derived iPSCs were plated in ultra-low-attachment microspace culture wells containing hundreds of U-bottom-shaped microwell spots per well to form cell aggregates in growth medium. The microwells had different aperture diameters/depths (400/560 <italic>μ</italic>m (Elp400), 500/700 <italic>μ</italic>m (Elp500), and 900/700 <italic>μ</italic>m (Elp900)) (Kuraray; Elplasia). After 5 days of aggregation, cells were maintained in osteogenic induction medium for 35 days. Only cells in the Elp500 condition tightly aggregated and maintained high viability during osteogenic induction. After 10 days of induction, Elp500 cell constructs showed significantly higher gene expression of <italic>Runx2</italic>, <italic>Osterix</italic>, <italic>Collagen 1a1</italic>, <italic>Osteocalcin</italic>, <italic>Bone sialoprotein</italic>, and <italic>Osteopontin</italic> compared to constructs in Elp400 and Elp900. In methylene blue-counterstained von Kossa staining and Movat’s pentachrome staining, only Elp500 constructs showed robust osteoid formation on day 35, with high expression of type I collagen (a major osteoid component) and osteocalcin proteins. Cell constructs were transplanted into rat calvarial bone defects, and micro-CT analysis after 3 weeks showed better bone repair with significantly higher bone mineral density in the Elp500 group compared to the Elp900 group. These results suggest that microspace size affects self-organized osteogenic differentiation of iPSCs. Elp500 microspace culture specifically induces mouse iPSCs into osteoid-rich bone-like tissue possessing high bone regeneration capacity.
Lipin-2 degradation elicits a proinflammatory gene signature in macrophages

Asami Watahiki, Kouhei Shimizu, Seira Hoshikawa, Mitsuki Chiba, Hiroshi Kitamura, Hiroshi Egusa, Satoshi Fukumoto, Hiroyuki Inuzuka

Biochemical and Biophysical Research Communications　524　(2)　477-483　2020/04

DOI： 10.1016/j.bbrc.2020.01.119 　

ISSN： 0006-291X

eISSN： 1090-2104
Silver diamine fluoride (SDF) used in childhood caries management has potent antifungal activity against oral Candida species Peer-reviewed

Kausar Sadia Fakhruddin, Hiroshi Egusa, Hien Chi Ngo, Chamila P, uwawala, Siripen Pesee, Thenmozhi Venkatachalam, Lakshman Perera Samaranayake

BMC Microbiol　20　(1)　95　2020/04
Publisher: Springer Science and Business Media {LLC}
DOI： 10.1186/s12866-020-01776-w 　

ISSN： 1471-2180
Implications of the circadian clock in implant dentistry Invited Peer-reviewed

Okawa H, Egusa H, Nishimura I

Dent Mater J　39　(2)　173-180　2020/03
Publisher: Japanese Society for Dental Materials and Devices
DOI： 10.4012/dmj.2019-291 　

ISSN： 0287-4547 1881-1361
Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits Peer-reviewed

Kausar Sadia Fakhruddin, Lakshman Perera Samaranayake, Hiroshi Egusa, Hien Chi Ngo, Chamila P, uwawala, Thenmozhi Venkatachalam, Allagappan Kumarappan, Siripen Pesee

J Oral Microbiol　12　(1)　1724484-1724484　2020/02
Publisher: Informa {UK} Limited
DOI： 10.1080/20002297.2020.1724484 　

ISSN： 2000-2297
High performance dental adhesives containing an ether-based monomer Peer-reviewed

Yamauchi S, Wang X, Egusa H, Sun J

J Dent Res　99　(2)　189-195　2020/02
Publisher: {SAGE} Publications
DOI： 10.1177/0022034519895269 　

ISSN： 0022-0345 1544-0591
Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs

Limraksasin, P., Okawa, H., Zhang, M., Kondo, T., Osathanon, T., Pavasant, P., Egusa, H.

Stem Cells International　2020　1-14　2020
Publisher: Hindawi Limited
DOI： 10.1155/2020/7082679 　

ISSN： 1687-9678
学生技能コンペティション「第1回JPS student clinical skills competition」 Peer-reviewed

峯篤史, 疋田一洋, 高場雅之, 澤瀬隆, 山下秀一郎, 榎並裕美子[田中], 鮎川保則, 上田貴之, 江草宏, 馬場俊輔, 笛木賢治, 槙原絵理, 横山紗和子, 大川周治, 馬場一美, 市川哲雄, 日本補綴歯科学会学術委員会

日本補綴歯科学会誌　12　(1)　5-15　2020/01
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
In vitro fabrication of hybrid bone/cartilage complex using mouse induced pluripotent stem cells Peer-reviewed

Limraksasin P, Kondo T, Zhang M, Okawa H, Osathanon T, Pavasant P, Egusa H

Int J Mol Sci　21　(2)　581-581　2020/01
Publisher: MDPI AG
DOI： 10.3390/ijms21020581 　

eISSN： 1422-0067

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Cell condensation and mechanical stimuli play roles in osteogenesis and chondrogenesis; thus, they are promising for facilitating self-organizing bone/cartilage tissue formation in vitro from induced pluripotent stem cells (iPSCs). Here, single mouse iPSCs were first seeded in micro-space culture plates to form 3-dimensional spheres. At day 12, iPSC spheres were subjected to shaking culture and maintained in osteogenic induction medium for 31 days (Os induction). In another condition, the osteogenic induction medium was replaced by chondrogenic induction medium at day 22 and maintained for a further 21 days (Os-Chon induction). Os induction produced robust mineralization and some cartilage-like tissue, which promoted expression of osteogenic and chondrogenic marker genes. In contrast, Os-Chon induction resulted in partial mineralization and a large area of cartilage tissue, with greatly increased expression of chondrogenic marker genes along with osterix and collagen 1a1. Os-Chon induction enhanced mesodermal lineage commitment with brachyury expression followed by high expression of lateral plate and paraxial mesoderm marker genes. These results suggest that combined use of micro-space culture and mechanical stimuli facilitates hybrid bone/cartilage tissue formation from iPSCs, and that the bone/cartilage tissue ratio in iPSC constructs could be manipulated through the induction protocol.
A possible risk of CAD/CAM-produced composite resin premolar crowns on a removable partial denture abutment tooth: A 3-year retrospective cohort study Peer-reviewed

Miura S, Kasahara S, Yamauchi S, Katsuda Y, Harada A, Aida J, Egusa H

J Prosthodont Res　63　(1)　78-84　2019

DOI： 10.1016/j.jpor.2018.08.005 　

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PURPOSE: To evaluate the early performance of computer-aided design/computer-aided manufacturing (CAD/CAM)-produced composite resin crown (CAD/CAM composite crown) treatment on premolars, specifically, placement on a removable partial denture (RPD) abutment tooth, and the distalmost tooth in the dental arch, as possible clinical risk factors. METHODS: A retrospective cohort study (April 2014 to July 2017) was performed utilizing the clinical records of patients who received a premolar CAD/CAM composite crown treatment. The variables of time of treatment for (1) successful crowns (complication event-free) and (2) surviving crowns (clinically functional including re-luted) were estimated using Kaplan-Meier analysis. Survival distributions regarding "RPD abutment tooth" and "distalmost tooth" were analyzed with the log-rank test. Multilevel survival analyses were used to identify hazard ratios and associated risk factors. RESULTS: Five hundred and forty-seven crowns were evaluated (mean follow-up time 1.3±0.9years) in 327 patients. A total of 87 crowns had at least one complication, with loss of crown retention being the most common (70 crowns). Estimated success and survival rates at 3 years were 71.7% and 96.4%, respectively. The risk of complications was significantly higher for an RPD abutment tooth than for a non-RPD abutment tooth. There was no significant difference between the distalmost tooth and non-distalmost tooth in the dental arch. CONCLUSIONS: The demonstrated complication rate for CAD/CAM composite crowns placed on premolars was 15.9% over a period of up to 3 years. There was a substantial risk of complications with placement of such a crown on an RPD abutment tooth.
Mesenchymal stem cell-based bone tissue engineering for veterinary practice Peer-reviewed

Nantavisai S, Egusa H, Osathanon T, Sawangmake C

Heliyon　5　(11)　e02808　2019
Publisher: Elsevier {BV}
DOI： 10.1016/j.heliyon.2019.e02808 　

ISSN： 2405-8440
Cytoprotective preconditioning of osteoblast-like cells with N-acetyl-L-cysteine for bone rgeneration in cell therapy Peer-reviewed

Yamada M, Watanabe J, Ueno T, Ogawa T, Egusa H

Int J Mol Sci　20　(20)　5199-5199　2019
Publisher: MDPI AG
DOI： 10.3390/ijms20205199 　

eISSN： 1422-0067

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Oxidative stress hinders tissue regeneration in cell therapy by inducing apoptosis and dysfunction in transplanted cells. N-acetyl-L-cysteine (NAC) reinforces cellular antioxidant capabilities by increasing a major cellular endogenous antioxidant molecule, glutathione, and promotes osteogenic differentiation. This study investigates the effects of pretreatment of osteoblast-like cells with NAC on oxidative stress-induced apoptosis and dysfunction and bone regeneration in local transplants. Rat femur bone marrow-derived osteoblast-like cells preincubated for 3 h with and without 5 mM NAC were cultured in a NAC-free osteogenic differentiation medium with continuous exposure to 50 μM hydrogen peroxide to induce oxidative stress. NAC preincubation prevented disruption of intracellular redox balance and alleviated apoptosis and negative impact on osteogenic differentiation, even under oxidative stress. Autologous osteoblast-like cells with and without NAC pretreatment in a collagen sponge vehicle were implanted in critical-size defects in rat femurs. In the third week, NAC-pretreated cells yielded complete defect closure with significantly matured lamellar bone tissue in contrast with poor bone healing by cells without pretreatment. Cell-tracking analysis demonstrated direct bone deposition by transplanted cells pretreated with NAC. Pretreatment of osteoblast-like cells with NAC enhances bone regeneration in local transplantation by preventing oxidative stress-induced apoptosis and dysfunction at the transplanted site.
Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor-β pathway Peer-reviewed

Manokawinchoke J, Pavasant P, Sawangmake C, Limjeerajarus N, Limjeerajarus CN, Egusa H, Osathanon T

Cell Death Dis　10　(10)　761　2019
Publisher: Springer Science and Business Media {LLC}
DOI： 10.1038/s41419-019-1992-4 　

ISSN： 2041-4889
RNA sequencing data of human periodontal ligament cells treated with continuous and intermittent compressive force Peer-reviewed

Manokawinchoke J, Pavasant P, Sawangmake C, Limjeerajarus N, Limjeerajarus CN, Egusa H, Osathanon T

Data Brief　26　104553-104553　2019
Publisher: Elsevier {BV}
DOI： 10.1016/j.dib.2019.104553 　

ISSN： 2352-3409
A thick frame decreases the fracture toughness of veneering ceramics used for zirconia-based all-ceramic restorations Peer-reviewed

Yamauchi S, Miura S, Kasahara S, Sun J, Egusa H

J Prosthodont Res　63　(2)　184-192　2019

DOI： 10.1016/j.jpor.2018.11.007 　

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PURPOSE: To investigate the influence of firing condition and thickness of a yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) frame on the fracture toughness of veneering ceramics. METHODS: Layering and pressable ceramics without frames or on 0.8- and 1.2-mm-thick Y-TZP frames were fired with 5 schedules (normal and 4 customized conditions). The fracture toughness of the ceramics was measured at 0.5-2.5mm from the frame by the indentation fracture method. Crystal structures at the measurement points were evaluated by X-ray diffraction (XRD) and scanning electron microscopy (SEM). RESULTS: Under customized firing conditions, the fracture toughness of the layering ceramic significantly decreased, particularly near the frame. Use of a 1.2-mm-thick frame significantly decreased the fracture toughness of both layering and pressable ceramics compared to ceramics without the frame. The decreased fracture toughness only occurred close (0.5 and 1.0mm) to the frame. XRD analysis showed identical diffraction patterns between points near and far from the frame, suggesting that the decreased fracture toughness was not due to crystal defects or impurities. SEM revealed many microcracks and large crystals in layering ceramic near the 1.2-mm-thick frame, possibly resulting from the thermal properties of the zirconia frame, such as large heat capacity and low thermal conductivity. CONCLUSIONS: Modification of the firing conditions from the manufacturer's instructions and inclusion of a thick Y-TZP frame decreased the fracture toughness of veneering ceramics at the interface, suggesting that a thick frame would pose a potent fracture risk in veneering ceramics used for zirconia-based all-ceramic restorations.
Influence of microscale expansion and contraction caused by thermal and mechanical fatigue on retentive strength of CAD/CAM-generated resin-based composite crowns. International-journal Peer-reviewed

Shuhei Ankyu, Keisuke Nakamura, Akio Harada, Ryoichi Inagaki, Yusuke Katsuda, Taro Kanno, Yoshimi Niwano, Ulf Örtengren, Hiroshi Egusa

Journal of the mechanical behavior of biomedical materials　86　(89)　89-97　2018/10

DOI： 10.1016/j.jmbbm.2018.06.025 　

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CAD/CAM-generated resin-based composite crowns have been proposed as an inexpensive alternative to conventional crowns. However, concerns have been raised about crown loosening in clinical use. Therefore, the present in vitro study aimed to evaluate the influence of thermal and mechanical cycling (TC and MC) on retentive strength of CAD/CAM resin-based crowns in relation to microscale expansion and contraction caused by fatigue. Eighty standardized dies were produced using a resin-based composite material. Crowns were milled from resin-based composite (n = 40) and glass-ceramic blocks (n = 40; control) using a dental CAD/CAM system. The crowns bonded to the dies were subjected to TC (temperature: 5 and 55 °C, cycles: 50,000) and MC (load: 200 N, cycles: 1.2 million). After fatigue treatment, retentive strength of the crowns was evaluated by a crown pull-off test at a crosshead speed of 1 mm/min. Coefficient of thermal expansion (CTE) and modulus of elasticity (E-modulus) of each material were also analyzed to estimate the microscale expansion and contraction during TC and MC. TC and MC significantly reduced the retentive strength of the CAD/CAM resin-based crowns whereas that of the CAD/CAM ceramic crowns was only affected by TC. In addition, the resin-based crowns showed a higher number of crown loosening during TC than the ceramic crowns. Analyses of CTE and E-modulus indicated that the resin-based crowns would be more deformed during TC and MC than the ceramic crowns. The present study demonstrated that the resistance of crowns to microscale expansion and contraction caused by thermal and mechanical fatigue would play an important role in maintaining retentive strength.
A novel pathway for the photooxidation of catechin in relation to its prooxidative activity. International-journal Peer-reviewed

Shunichi Shishido, Rei Miyano, Takuji Nakashima, Hirotaka Matsuo, Masato Iwatsuki, Keisuke Nakamura, Taro Kanno, Hiroshi Egusa, Yoshimi Niwano

Scientific reports　8　(1)　12888-12888　2018/08/27

DOI： 10.1038/s41598-018-31195-x 　

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In the present study, we evaluated the prooxidative mode of action of photoirradiated (+)-catechin at 400 nm in relation to reactive oxygen species generation and its possible application to disinfection. Photoirradiation of (+)-catechin at a concentration of 1 mg/mL yielded not only hydrogen peroxide (H2O2) but hydroxyl radical (·OH) in a total amount of approximately 20 μM in 10 min. As a result, photoirradiated catechin killed Staphylococcus aureus, and a > 5-log reduction in viable bacteria counts was observed within 20 min. Liquid chromatography-high-resolution-electrospray ionization-mass spectrometry showed that photoirradiation decreased the (+)-catechin peak (molecular formula C15H14O6) whilst it increased two peaks of a substance with the molecular formula C15H12O6 with increasing irradiation time. Nuclear magnetic resonance analysis revealed that the two C15H12O6 peaks were allocated to intramolecular cyclization products that are enantiomers of each other. These results suggest that photoirradiation induces oxidation of (+)-catechin resulting in the reduction of oxygen to generate H2O2. This H2O2 is then homolytically cleaved to ·OH, and alongside this process, (+)-catechin is finally converted to two intramolecular cyclization products that are different from the quinone structure of the B ring, as proposed previously for the autoxidation and enzymatic oxidation of catechins.
Current bone substitutes for implant dentistry Peer-reviewed

Masahiro Yamada, Hiroshi Egusa

Journal of Prosthodontic Research　62　(2)　152-161　2018/04/01
Publisher: Elsevier Ltd
DOI： 10.1016/j.jpor.2017.08.010 　

ISSN： 1883-1958
Characterization of a bioactive Jagged1-coated polycaprolactone-based membrane for guided tissue regeneration Peer-reviewed

Nunthawan Nowwarote, Phunphimp Chanjavanakul, Pattanit Kongdecha, Panipuk Clayhan, Sujin Chumprasert, Jeeranan Manokawinchoke, Hiroshi Egusa, Prasit Pavasant, Thanaphum Osathanon

Archives of Oral Biology　88　24-33　2018/04/01
Publisher: Elsevier Ltd
DOI： 10.1016/j.archoralbio.2018.01.007 　

ISSN： 1879-1506 0003-9969
Effect of finish line design on stress distribution in bilayer and monolithic zirconia crowns: a three-dimensional finite element analysis study Peer-reviewed

Shoko Miura, Shin Kasahara, Shinobu Yamauchi, Hiroshi Egusa

European Journal of Oral Sciences　126　(2)　159-165　2018/04/01
Publisher: Blackwell Munksgaard
DOI： 10.1111/eos.12402 　

ISSN： 1600-0722 0909-8836
Binding of PICK1 PDZ domain with calcineurin B regulates osteoclast differentiation Peer-reviewed

Yuya Kamano, Jun Watanabe, Tsutomu Iida, Takeru Kondo, Hiroko Okawa, Hirofumi Yatani, Makio Saeki, Hiroshi Egusa

Biochemical and Biophysical Research Communications　496　(1)　83-88　2018/01/29
Publisher: Elsevier B.V.
DOI： 10.1016/j.bbrc.2017.12.173 　

ISSN： 1090-2104 0006-291X
Preconditioning of bone marrow-derived mesenchymal stem cells with N-acetyl-L-cysteine enhances bone regeneration via reinforced resistance to oxidative stress International-journal Peer-reviewed

Watanabe J, Yamada M, Niibe K, Maolin Z, Kondo T, Ishibashi M, Egusa H

Biomaterials　185　25-38　2018

DOI： 10.1016/j.biomaterials.2018.08.055 　

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Oxidative stress on transplanted bone marrow-derived mesenchymal stem cells (BMSCs) during acute inflammation is a critical issue in cell therapies. N-acetyl-L cysteine (NAC) promotes the production of a cellular antioxidant molecule, glutathione (GSH). The aim of this study was to investigate the effects of pre-treatment with NAC on the apoptosis resistance and bone regeneration capability of BMSCs. Rat femur-derived BMSCs were treated in growth medium with or without 5 mM NAC for 6 h, followed by exposure to 100 μM H2O2 for 24 h to induce oxidative stress. Pre-treatment with NAC significantly increased intracellular GSH levels by up to two fold and prevented H2O2-induced intracellular redox imbalance, apoptosis and senescence. When critical-sized rat femur defects were filled with a collagen sponge containing fluorescent-labeled autologous BMSCs with or without NAC treatment, the number of apoptotic and surviving cells in the transplanted site after 3 days was significantly lower and higher in the NAC pre-treated group, respectively. By the 5th week, significantly enhanced new bone formation was observed in the NAC pre-treated group. These data suggest that pre-treatment of BMSCs with NAC before local transplantation enhances bone regeneration via reinforced resistance to oxidative stress-induced apoptosis at the transplanted site.
補綴歯科領域で期待される幹細胞 Peer-reviewed

新部邦透, 江草宏

日本補綴歯科学会誌　10　(3)　230-237　2018
歯周病患者に対する補綴歯科治療の専門性－『米国型 vs. スカンジナビア型』を読んで－ Peer-reviewed

江草宏

日本補綴歯科学会誌　10　(3)　202-208　2018
『米国型 vs. スカンジナビア型』－日本の補綴歯科専門医はどちらを向いているのか？－ Peer-reviewed

江草宏

日本補綴歯科学会誌　10　(2)　134-135　2018
東北大学歯学部におけるCAD/CAM冠模型実習システムの導入 Peer-reviewed

勝田悠介, 山田将博, 石橋実, 奥山弥生, 江草宏

日本補綴歯科学会誌　10　(4)　335-344　2018
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
The effects of platelet-derived growth factor-BB on bone marrow stromal cell-mediated vascularized bone regeneration Peer-reviewed

Maolin Zhang, Wenwen Yu, Kunimichi Niibe, Wenjie Zhang, Hiroshi Egusa, Tingting Tang, Xinquan Jiang

Stem Cells Int　Article ID 3272098　1-16　2018
Publisher: Hindawi Limited
DOI： 10.1155/2018/3272098 　

ISSN： 1687-966X 1687-9678
Prolonged release of bone morphogenetic protein-2 in vivo by gene transfection with DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds Peer-reviewed

Tenkumo T, Vanegas Sáenz JR, Nakamura K, Shimizu Y, Sokolova V, Epple M, Kamano Y, Egusa H, Sugaya T, Sasaki K

Mater Sci Eng C Mater Biol Appl　92　172-183　2018

DOI： 10.1016/j.msec.2018.06.047 　

ISSN： 0928-4931

eISSN： 1873-0191
RNA sequencing data of Notch ligand treated human dental pulp cells Peer-reviewed

Manokawinchoke J, Nattasit P, Thongngam T, Pavasant P, Tompkins KA, Egusa H, Osathanon T

Data Brief　17　407-413　2018
Publisher: Elsevier {BV}
DOI： 10.1016/j.dib.2018.01.058 　

ISSN： 2352-3409
Clinical evaluation of zirconia-based all-ceramic single crowns: an up to 12-year retrospective cohort study International-journal Peer-reviewed

Miura S, Kasahara S, Yamauchi S, Okuyama Y, Izumida A, Aida J, Egusa H

Clin Oral Investig　22　(2)　697-706　2018

DOI： 10.1007/s00784-017-2142-y 　

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OBJECTIVES: This study aims to investigate the incidence of clinical complications with tooth-supported zirconia-based all-ceramic single crowns and identify pertinent risk parameters. MATERIALS AND METHODS: A retrospective cohort study (May 2004 to April 2016) utilizing clinical records of patients receiving yttrium-oxide-partially stabilized zirconia (Y-TZP)-based all-ceramic crowns placed at Tohoku University Hospital was performed. The length of time of treatment success (complication event-free) and restoration survival (including minor complication events and remaining clinically functional) were estimated using Kaplan-Meier analysis. Multilevel survival analysis was used to identify risk factors. RESULTS: One hundred thirty-seven crowns were evaluated (mean follow-up time, 7.0 years). A total of 21 crowns experienced at least one complication with fracture of veneering ceramic being the most common (16 crowns). Estimated success and survival rates at 5 years (96.9 and 98.5%, respectively) decreased at 10 years to 62.1 and 67.2%, respectively. The risk of complications was significantly higher for molar crowns compared to anterior crowns (p < 0.01). A significant association of complications with metal antagonist restorations was shown by univariate analysis (p < 0.01). CONCLUSIONS: Given the study limitations, Y-TZP single crowns placed on anterior teeth demonstrated encouraging clinical results over a period of up to 10 years. However, there is a substantial risk of complications with posterior teeth within 10 years of restoration placement. CLINICAL RELEVANCE: Treatment with zirconia-based all-ceramic crowns for molar teeth with metal antagonist occlusion should be undertaken with caution.
歯科用ユニットの水質管理－“フラッシング”の重要性－ Invited Peer-reviewed

山田将博, 鷲尾純平, 高橋信博, 江草宏

日本歯科理工学会誌　37　(4)　223-226　2018
Publisher: (一社)日本歯科理工学会
ISSN： 1884-4421
Surface properties of dental zirconia ceramics affected by ultrasonic scaling and low-temperature degradation. International-journal Peer-reviewed

Kosuke Nakazawa, Keisuke Nakamura, Akio Harada, Midori Shirato, Ryoichi Inagaki, Ulf Örtengren, Taro Kanno, Yoshimi Niwano, Hiroshi Egusa

PloS one　13　(9)　e0203849　2018

DOI： 10.1371/journal.pone.0203849 　

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Zirconia (3Y-TZP) dental prostheses are widely used in clinical dentistry. However, the effect of ultrasonic scaling performed as a part of professional tooth cleaning on 3Y-TZP dental prostheses, especially in conjunction with low-temperature degradation (LTD), has not been fully investigated. The present study aimed to evaluate the influence of ultrasonic scaling and LTD on the surface properties of 3Y-TZP in relation to bacterial adhesion on the treated surface. 3Y-TZP specimens (4 × 4 × 2 mm) were polished and then subjected to autoclaving at 134°C for 100 h to induce LTD, followed by 10 rounds of ultrasonic scaling using a steel scaler tip for 1 min each. Surface roughness, crystalline structure, wettability, and hardness were analyzed by optical interferometry, X-ray diffraction analysis, contact angle measurement, and nano-indentation technique, respectively. Subsequently, bacterial adhesion onto the treated 3Y-TZP surface was evaluated using Streptococcus mitis and S. oralis. The results demonstrated that the combination of ultrasonic scaling and LTD significantly increased the Sa value (surface roughness parameter) of the polished 3Y-TZP surface from 1.6 nm to 117 nm. LTD affected the crystalline structure, causing phase transformation from the tetragonal to the monoclinic phase, and decreased both the contact angle and surface hardness. However, bacterial adhesion was not influenced by these changes in surface properties. The present study suggests that ultrasonic scaling may be acceptable for debridement of 3Y-TZP dental prostheses because it did not facilitate bacterial adhesion even in the combination with LTD, although it did cause slight roughening of the surface.
Amiloride-enhanced gene transfection of octa-arginine functionalized calcium phosphate nanoparticles Peer-reviewed

Juan Ramon Vanegas Saenz, Taichi Tenkumo, Yuya Kamano, Hiroshi Egusa, Keiichi Sasaki

PLOS ONE　12　(11)　e0188347　2017/11

DOI： 10.1371/journal.pone.0188347 　

ISSN： 1932-6203
Notch signaling partly regulates the osteogenic differentiation of retinoic acid-treated murine induced pluripotent stem cells Peer-reviewed

Thanaphum Osathanon, Jeeranan Manokawinchoke, Hiroshi Egusa, Prasit Pavasant

JOURNAL OF ORAL SCIENCE　59　(3)　405-413　2017/09

DOI： 10.2334/josnusd.16-0552 　

ISSN： 1343-4934

eISSN： 1880-4926
Indirect immobilized Jagged1 suppresses cell cycle progression and induces odonto/osteogenic differentiation in human dental pulp cells Peer-reviewed

Jeeranan Manokawinchoke, Praphawi Nattasit, Tanutchaporn Thongngam, Prasit Pavasant, Kevin A. Tompkins, Hiroshi Egusa, Thanaphum Osathanon

SCIENTIFIC REPORTS　7　(1)　10124　2017/08

DOI： 10.1038/s41598-017-10638-x 　

ISSN： 2045-2322
Progress of the Journal of Prosthodontic Research under the four-year action plan Invited Peer-reviewed

Hiroshi Egusa

JOURNAL OF PROSTHODONTIC RESEARCH　61　(3)　231-232　2017/07

DOI： 10.1016/j.jpor.2017.06.003 　

ISSN： 1883-1958

eISSN： 2212-4632
Activation of Osteoblastic Function on Titanium Surface with Titanium-Doped Hydroxyapatite Nanoparticle Coating: An In Vitro Study Peer-reviewed

Masahiro Nakazawa, Masahiro Yamada, Masato Wakamura, Hiroshi Egusa, Kaoru Sakurai

INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS　32　(4)　779-791　2017/07

DOI： 10.11607/jomi.5421 　

ISSN： 0882-2786

eISSN： 1942-4434
Three-dimensional finite element analysis of zirconia all-ceramic cantilevered fixed partial dentures with different framework designs Peer-reviewed

Shoko Miura, Shin Kasahara, Shinobu Yamauchi, Hiroshi Egusa

EUROPEAN JOURNAL OF ORAL SCIENCES　125　(3)　208-214　2017/06

DOI： 10.1111/eos.12342 　

ISSN： 0909-8836

eISSN： 1600-0722
The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry Peer-reviewed

Kunimichi Niibe, Maolin Zhang, Kosuke Nakazawa, Satoru Morikawa, Taneaki Nakagawa, Yumi Matsuzaki, Hiroshi Egusa

Japanese Dental Science Review　53　(2)　25-33　2017/05/01
Publisher: Elsevier Ltd
DOI： 10.1016/j.jdsr.2016.09.001 　

ISSN： 2213-6851 1882-7616
Challenges for stem cell-based "regenerative prosthodontics" Peer-reviewed

Kunimichi Niibe, Fumio Suehiro, Masamitsu Oshima, Masahiro Nishimura, Takuo Kuboki, Hiroshi Egusa

JOURNAL OF PROSTHODONTIC RESEARCH　61　(1)　3-5　2017/01

DOI： 10.1016/j.jpor.2016.09.001 　

ISSN： 1883-1958

eISSN： 2212-4632
Efficacy and safety of a therapeutic apparatus using hydrogen peroxide photolysis to treat dental and periodontal infectious diseases Peer-reviewed

Hirohisa Sato, Yoshimi Niwano, Keisuke Nakamura, Takayuki Mokudai, Hiroyo Ikai, Taro Kanno, Hiroshi Egusa

JOURNAL OF TOXICOLOGICAL SCIENCES　41　(6)　793-799　2016/12

DOI： 10.2131/jts.41.793 　

ISSN： 0388-1350

eISSN： 1880-3989
Hypoxia enhances osteogenic differentiation in retinoic acid-treated murine-induced pluripotent stem cells Peer-reviewed

Jeeranan Manokawinchoke, Thanaphum Osathanon, Hiroshi Egusa, Prasit Pavasant

TISSUE ENGINEERING AND REGENERATIVE MEDICINE　13　(5)　547-553　2016/10

DOI： 10.1007/s13770-016-9127-9 　

ISSN： 1738-2696
Fatigue analysis of computer-aided design/computer-aided manufacturing resin-based composite vs. lithium disilicate glass-ceramic Peer-reviewed

Shuhei Ankyu, Keisuke Nakamura, Akio Harada, Guang Hong, Taro Kanno, Yoshimi Niwano, Ulf Ortengren, Hiroshi Egusa

EUROPEAN JOURNAL OF ORAL SCIENCES　124　(4)　387-395　2016/08

DOI： 10.1111/eos.12278 　

ISSN： 0909-8836

eISSN： 1600-0722
Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model Peer-reviewed

Ryoji Takagi, Junko Ishimaru, Ayaka Sugawara, Koh-ei Toyoshima, Kentaro Ishida, Miho Ogawa, Kei Sakakibara, Kyosuke Asakawa, Akitoshi Kashiwakura, Masamitsu Oshima, Ryohei Minamide, Akio Sato, Toshihiro Yoshitake, Akira Takeda, Hiroshi Egusa, Takashi Tsuji

SCIENCE ADVANCES　2　(4)　e1500887　2016/04

DOI： 10.1126/sciadv.1500887 　

ISSN： 2375-2548
The inhibitors of cyclin-dependent kinases and GSK-3β enhance osteoclastogenesis Peer-reviewed

Yosuke Akiba, Akiko Mizuta, Yoshito Kakihara, Juri Nakata, Jun Nihara, Isao Saito, Hiroshi Egusa, Makio Saeki

Biochemistry and Biophysics Reports　5　253-258　2016/03/01

DOI： 10.1016/j.bbrep.2015.12.011 　

eISSN： 2405-5808
Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells Peer-reviewed

Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

STEM CELLS INTERNATIONAL　Article ID 6240794　2016

DOI： 10.1155/2016/6240794 　

ISSN： 1687-966X

eISSN： 1687-9678
Gingival Fibroblasts as Autologous Feeders for Induced Pluripotent Stem Cells Peer-reviewed

G. Yu, H. Okawa, K. Okita, Y. Kamano, F. Wang, M. Saeki, H. Yatani, H. Egusa

JOURNAL OF DENTAL RESEARCH　95　(1)　110-118　2016/01

DOI： 10.1177/0022034515611602 　

ISSN： 0022-0345

eISSN： 1544-0591
Another year of growth for the Journal of Prosthodontic Research Invited Peer-reviewed

Hiroshi Egusa

JOURNAL OF PROSTHODONTIC RESEARCH　60　(1)　1-2　2016/01

DOI： 10.1016/j.jpor.2015.12.002 　

ISSN： 1883-1958

eISSN： 2212-4632
Controlled Osteogenic Differentiation of Mouse Mesenchymal Stem Cells by Tetracycline-Controlled Transcriptional Activation of Amelogenin Peer-reviewed

Fangfang Wang, Hiroko Okawa, Yuya Kamano, Kunimichi Niibe, Hiroki Kayashima, Thanaphum Osathanon, Prasit Pavasant, Makio Saeki, Hirofumi Yatani, Hiroshi Egusa

PLOS ONE　10　(12)　e0145677　2015/12

DOI： 10.1371/journal.pone.0145677 　

ISSN： 1932-6203
Screening of Pharmacologically Active Small Molecule Compounds Identifies Antifungal Agents Against Candida Biofilms Peer-reviewed

Takao Watamoto, Hiroshi Egusa, Takashi Sawase, Hirofumi Yatani

FRONTIERS IN MICROBIOLOGY　6　1453　2015/12

DOI： 10.3389/fmicb.2015.01453 　

ISSN： 1664-302X
Cutting efficiency of diamond burs operated with electric high-speed dental handpiece on zirconia Peer-reviewed

Keisuke Nakamura, Yusuke Katsuda, Shuhei Ankyu, Akio Harada, Taichi Tenkumo, Taro Kanno, Yoshimi Niwano, Hiroshi Egusa, Percy Milleding, Ulf Ortengren

EUROPEAN JOURNAL OF ORAL SCIENCES　123　(5)　375-380　2015/10

DOI： 10.1111/eos.12211 　

ISSN： 0909-8836

eISSN： 1600-0722
Cytoprotective Effects of Grape Seed Extract on Human Gingival Fibroblasts in Relation to Its Antioxidant Potential Peer-reviewed

Yusuke Katsuda, Yoshimi Niwano, Takuji Nakashima, Takayuki Mokudai, Keisuke Nakamura, Satomi Oizumi, Taro Kanno, Hiroyasu Kanetaka, Hiroshi Egusa

PLOS ONE　10　(8)　e0134704　2015/08

DOI： 10.1371/journal.pone.0134704 　

ISSN： 1932-6203
PIH1D1 positively regulates mTOR complex 1-dependent rRNA transcription Peer-reviewed

Kamano Yuya, Saeki Makio, Kamisaki Yoshinori, Egusa Hiroshi

JOURNAL OF PHARMACOLOGICAL SCIENCES　128　(3)　S120　2015/07

ISSN： 1347-8613
Biodental engineering Invited Peer-reviewed

Takuya Matsumoto, Hiroshi Egusa, Koichi Kato, Takashi Tsuji

Journal of Oral Biosciences　57　(2)　80-85　2015/05/01
Publisher: Japanese Association for Oral Biology
DOI： 10.1016/j.job.2015.01.002 　

ISSN： 1349-0079
Update zirconia restorations Peer-reviewed

Takuro Takeichi, Shoko Miura, Shin Kasahara, Hiroshi Egusa, Mai Hara, Toru Sato, Masao Yoshinari, Tetsurou Odatsu, Ikuya Watanabe, Takashi Sawase

JOURNAL OF PROSTHODONTIC RESEARCH　59　(2)　81-83　2015/04

DOI： 10.1016/j.jpor.2014.11.005 　

ISSN： 1883-1958

eISSN： 2212-4632
Fracture resistance of computer-aided design/computer-aided manufacturing generated composite resin-based molar crowns Peer-reviewed

Akio Harada, Keisuke Nakamura, Taro Kanno, Ryoichi Inagaki, Ulf Ortengren, Yoshimi Niwano, Keiichi Sasaki, Hiroshi Egusa

EUROPEAN JOURNAL OF ORAL SCIENCES　123　(2)　122-129　2015/04

DOI： 10.1111/eos.12173 　

ISSN： 0909-8836

eISSN： 1600-0722
Cyclic mechanical pressure-loading alters epithelial homeostasis in a three-dimensional in vitro oral mucosa model: clinical implications for denture-wearers Peer-reviewed

A. Shiomi, K. Izumi, A. Uenoyama, T. Saito, N. Saito, H. Ohnuki, H. Kato, M. Kanatani, S. Nomura, H. Egusa, T. Maeda

JOURNAL OF ORAL REHABILITATION　42　(3)　192-201　2015/03

DOI： 10.1111/joor.12254 　

ISSN： 0305-182X

eISSN： 1365-2842
Synergistic interaction between wavelength of light and concentration of H2O2 in bactericidal activity of photolysis of H2O2 Peer-reviewed

Toshihide Toki, Keisuke Nakamura, Michiko Kurauchi, Taro Kanno, Yusuke Katsuda, Hiroyo Ikai, Eisei Hayashi, Hiroshi Egusa, Keiichi Sasaki, Yoshimi Niwano

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　119　(3)　358-362　2015/03

DOI： 10.1016/j.jbiosc.2014.08.015 　

ISSN： 1389-1723

eISSN： 1347-4421
Clinical chipping of zirconia all-ceramic restorations

Miura, S., Kasahara, S., Kudo, M., Okuyama, Y., Yoda, M., Egusa, H.

Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent Interface　2015

DOI： 10.1007/978-4-431-55192-8_27 　
Erratum: Cytoprotective effects of grape seed extract on human gingival fibroblasts in relation to its antioxidant potential (PLoS ONE (2015) 10:8 DOI:10.1371/journal.pone.0134704)

Katsuda, Y., Niwano, Y., Nakashima, T., Mokudai, T., Nakamura, K., Oizumi, S., Kanno, T., Kanetaka, H., Egusa, H.

PLoS ONE　10　(9)　2015
Publisher: Public Library of Science ({PLoS})
DOI： 10.1371/journal.pone.0138394 　

ISSN： 1932-6203
Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells. International-journal Peer-reviewed

Hitomi Ono, Aya Obana, Yu Usami, Manabu Sakai, Kanji Nohara, Hiroshi Egusa, Takayoshi Sakai

BioMed research international　2015　293570-293570　2015

DOI： 10.1155/2015/293570 　

ISSN： 2314-6133

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This report describes our initial attempt to regenerate salivary glands using induced pluripotent stem (iPS) cells in vivo and in vitro. Glandular tissues that were similar to the adult submandibular glands (SMGs) and sublingual glands could be partially produced by the transplantation of iPS cells into mouse salivary glands. However, the tumorigenicity of iPS cells has not been resolved yet. It is well known that stem cells affect their microenvironment, known as a stem cell niche. We focused on the niche and the interaction between iPS cells and salivary gland cells in our study on salivary gland regeneration. Coculture of embryonic SMG cells and iPS cells have better-developed epithelial structures and fewer undifferentiated specific markers than monoculture of embryonic SMG cells in vitro. These results suggest that iPS cells have a potential ability to accelerate differentiation for salivary gland development and regeneration.
Expression of neuropeptide receptor mRNA during osteoblastic differentiation of mouse iPS cells Peer-reviewed

Satomi Nagao, Tetsuya Goto, Shinji Kataoka, Takashi Toyono, Takaaki Joujima, Hiroshi Egusa, Hirofumi Yatani, Shigeru Kobayashi, Kenshi Maki

NEUROPEPTIDES　48　(6)　399-406　2014/12

DOI： 10.1016/j.npep.2014.10.004 　

ISSN： 0143-4179

eISSN： 1532-2785
Novel strategies for the development of anabolic agents for treatment of osteoporosis Peer-reviewed

Saeki M, Egusa H

Nihon yakurigaku zasshi. Folia pharmacologica Japonica　144　(6)　277-280　2014/12
Publisher: The Japanese Pharmacological Society
DOI： 10.1254/fpj.144.277 　

ISSN： 0015-5691
Acceleration of Proliferative Response of Mouse Fibroblasts by Short-Time Pretreatment with Polyphenols Peer-reviewed

Makoto Tsuruya, Yoshimi Niwano, Keisuke Nakamura, Taro Kanno, Takuji Nakashima, Hiroshi Egusa, Keiichi Sasaki

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY　174　(6)　2223-2235　2014/11

DOI： 10.1007/s12010-014-1124-7 　

ISSN： 0273-2289

eISSN： 1559-0291
Cytoprotective Effect of Short-Term Pretreatment with Proanthocyanidin on Human Gingival Fibroblasts Exposed to Harsh Environmental Conditions Peer-reviewed

Michiko Kurauchi, Yoshimi Niwano, Midori Shirato, Taro Kanno, Keisuke Nakamura, Hiroshi Egusa, Keiichi Sasaki

PLOS ONE　9　(11)　e113403　2014/11

DOI： 10.1371/journal.pone.0113403 　

ISSN： 1932-6203
Increasing diversity in prosthodontic research Peer-reviewed

Hiroshi Egusa

JOURNAL OF PROSTHODONTIC RESEARCH　58　(4)　191-192　2014/10

DOI： 10.1016/j.jpor.2014.08.002 　

ISSN： 1883-1958

eISSN： 2212-4632
Comparative Analysis of Mouse-Induced Pluripotent Stem Cells and Mesenchymal Stem Cells During Osteogenic Differentiation In Vitro Peer-reviewed

Hiroshi Egusa, Hiroki Kayashima, Jiro Miura, Shinya Uraguchi, Fangfang Wang, Hiroko Okawa, Jun-Ichi Sasaki, Makio Saeki, Takuya Matsumoto, Hirofumi Yatani

STEM CELLS AND DEVELOPMENT　23　(18)　2156-2169　2014/09

DOI： 10.1089/scd.2013.0344 　

ISSN： 1547-3287

eISSN： 1557-8534
Allergic Reaction to Titanium-Made Fixed Dental Restorations: A Clinical Report Peer-reviewed

Nagakazu Ko, Atsushi Mine, Hiroshi Egusa, Tsunetoshi Shimazu, Rieko Ko, Tamaki Nakano, Hirofumi Yatani

JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY　23　(6)　501-503　2014/08

DOI： 10.1111/jopr.12136 　

ISSN： 1059-941X

eISSN： 1532-849X
歯肉を用いた医療応用に安全なiPS 細胞の開発 ―新たな再生歯科医療技術の創成に向けて― Invited

江草宏, 于冠男, 鎌野優弥, 大川博子, 山本治毅, 矢谷博文

日歯医学会誌　33　54-58　2014/03
Application of a Pin Technique to a Combination Design of Conventional and Resin-Bonded Prosthesis in the Maxillary Anterior Region: A Case Report Peer-reviewed

Egusa Hiroshi

Annals of Japan Prosthodontic Society　6　(1)　71-74　2014/01
Publisher: Japan Prosthodontic Society
DOI： 10.2186/ajps.6.71 　

ISSN： 1883-4426

More details Close

Patient: An 18-year-old male visited our department with a masticatory disturbance arising from a root fracture in the maxillary left central incisor, which had to be extracted. For bridge treatment, a full-coverage porcelain fused to a metal crown was applied to the abutment tooth of the maxillary left lateral incisor because the tooth was a restored microdont. The other abutment tooth of the maxillary right central incisor was an intact tooth; therefore, a resin-bonded retainer was applied using a pin technique to strengthen the retention. Discussion: Over 7 years of follow-up, no prosthetic complications were observed, and the patient was satisfied with the treatment outcome. Conclusion: In the case of a resin-bonded prosthesis containing a one-sided full-coverage crown, the combined use of pin and resin-bonded techniques on the other adhesion retainer enabled minimal intervention dentistry and long-term retention of the prosthesis and resulted in high patient satisfaction.
Clinical Evaluation of Chlorine Dioxide for Disinfection of Dental Instruments Peer-reviewed

Takao Watamoto, Hiroshi Egusa, Takashi Sawase, Hirofumi Yatani

INTERNATIONAL JOURNAL OF PROSTHODONTICS　26　(6)　541-544　2013/11

DOI： 10.11607/ijp.3465 　

ISSN： 0893-2174

eISSN： 1942-4426
PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA transcription Peer-reviewed

Yuya Kamano, Makio Saeki, Hiroshi Egusa, Yoshito Kakihara, Walid A. Houry, Hirofumi Yatani, Yoshinori Kamisaki

FEBS Letters　587　(20)　3303-3308　2013/10/11

DOI： 10.1016/j.febslet.2013.09.001 　

ISSN： 0014-5793 1873-3468
iPS細胞の骨生物学への応用と歯科臨床への展望 Invited

江草宏

医学生物学電子顕微鏡技術学会誌　27　(2)　67-69　2013/10
Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA Peer-reviewed

Makio Saeki, Hiroshi Egusa, Yuya Kamano, Yoshito Kakihara, Walid A. Houry, Hirofumi Yatani, Shinzaburo Noguchi, Yoshinori Kamisaki

PLOS ONE　8　(7)　e67326　2013/07

DOI： 10.1371/journal.pone.0067326 　

ISSN： 1932-6203
Application of Cyclic Strain for Accelerated Skeletal Myogenic Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stromal Cells with Cell Alignment Peer-reviewed

Hiroshi Egusa, Munemasa Kobayashi, Takuya Matsumoto, Jun-Ichi Sasaki, Shinya Uraguchi, Hirofumi Yatani

TISSUE ENGINEERING PART A　19　(5-6)　770-782　2013/03

DOI： 10.1089/ten.tea.2012.0164 　

ISSN： 1937-3341
RPAP3 splicing variant isoform 1 interacts with PIH1D1 to compose R2TP complex for cell survival Peer-reviewed

Miki Yoshida, Makio Saeki, Hiroshi Egusa, Yasuyuki Irie, Yuya Kamano, Shinya Uraguchi, Maki Sotozono, Hitoshi Niwa, Yoshinori Kamisaki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　430　(1)　320-324　2013/01

DOI： 10.1016/j.bbrc.2012.11.017 　

ISSN： 0006-291X

eISSN： 1090-2104
Frontier dental research on iPS cells Invited Peer-reviewed

Makiko Arakaki, Hiroshi Egusab, Keishi Otsuc, Issei Saitoh, Takumi Miurae, Hidemitsu Haradac

Journal of Oral Biosciences　55　(4)　191-199　2013
Publisher: Japanese Association for Oral Biology
DOI： 10.1016/j.job.2013.08.002 　

ISSN： 1349-0079
Stem cells in dentistry - Part II: Clinical applications Peer-reviewed

Hiroshi Egusa, Wataru Sonoyama, Masahiro Nishimura, Ikiru Atsuta, Kentaro Akiyama

JOURNAL OF PROSTHODONTIC RESEARCH　56　(4)　229-248　2012/10

DOI： 10.1016/j.jpor.2012.10.001 　

ISSN： 1883-1958

eISSN： 2212-4632
Stem cells in dentistry - Part I: Stem cell sources Peer-reviewed

Hiroshi Egusa, Wataru Sonoyama, Masahiro Nishimura, Ikiru Atsuta, Kentaro Akiyama

JOURNAL OF PROSTHODONTIC RESEARCH　56　(3)　151-165　2012/07

DOI： 10.1016/j.jpor.2012.06.001 　

ISSN： 1883-1958

eISSN： 2212-4632
Kruppel-like factor 4 regulates membranous and endochondral ossification Peer-reviewed

Ikumi Michikami, Toshiya Fukushi, Mariko Tanaka, Hiroshi Egusa, Yoshinobu Maeda, Takashi Ooshima, Satoshi Wakisaka, Makoto Abe

EXPERIMENTAL CELL RESEARCH　318　(4)　311-325　2012/02

DOI： 10.1016/j.yexcr.2011.12.013 　

ISSN： 0014-4827
iPS cells in dentistry Invited

EGUSA Hiroshi

Clinical Calcium　22　(1)　67-73　2012/01

ISSN： 0917-5857
In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct Peer-reviewed

Jun-Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Michiya Matsusaki, Akihiro Nishiguchi, Takayoshi Nakano, Mitsuru Akashi, Satoshi Imazato, Hirofumi Yatani

INTEGRATIVE BIOLOGY　4　(10)　1207-1214　2012

DOI： 10.1039/c2ib20027a 　

ISSN： 1757-9694
Hydrogel-based biomimetic environment for in vitro modulation of branching morphogenesis Peer-reviewed

Hiroyuki Miyajima, Takuya Matsumoto, Takayoshi Sakai, Satoshi Yamaguchi, Sang Hyun An, Makoto Abe, Satoshi Wakisaka, Kuen Yong Lee, Hiroshi Egusa, Satoshi Imazato

BIOMATERIALS　32　(28)　6754-6763　2011/10

DOI： 10.1016/j.biomaterials.2011.05.072 　

ISSN： 0142-9612
Transcriptional regulation of drug-resistance genes in Candida albicans biofilms in response to antifungals Peer-reviewed

T. Watamoto, L. P. Samaranayake, H. Egusa, H. Yatani, C. J. Seneyiratne

JOURNAL OF MEDICAL MICROBIOLOGY　60　(9)　1241-1247　2011/09

DOI： 10.1099/jmm.0.030692-0 　

ISSN： 0022-2615
The small molecule harmine regulates NFATc1 and Id2 expression in osteoclast progenitor cells Peer-reviewed

Hiroshi Egusa, Masanori Doi, Makio Saeki, Sho Fukuyasu, Yoshihiro Akashi, Yoshifumi Yokota, Hirofumi Yatani, Yoshinori Kamisaki

BONE　49　(2)　264-274　2011/08

DOI： 10.1016/j.bone.2011.04.003 　

ISSN： 8756-3282
RPAP3 enhances cytotoxicity of doxorubicin by impairing NF-kappa B pathway Peer-reviewed

Kana Shimada, Makio Saeki, Hiroshi Egusa, Sho Fukuyasu, Yoshiaki Yura, Kazuhiro Iwai, Yoshinori Kamisaki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　404　(4)　910-914　2011/01

DOI： 10.1016/j.bbrc.2010.12.071 　

ISSN： 0006-291X
ハイドロゲルを応用した三次元配向組織のin vitro構築 Invited

佐々木淳一, 松本卓也, 江草宏, 矢谷博文

大阪大学歯学会雑誌　55　(2)　83-89　2011

ISSN： 0473-4629
歯肉のあらたな可能性－医療応用に有望なiPS細胞源－ Invited

江草宏

大阪大学歯学雑誌　56　(1)　31-40　2011
Influence of titanium oxide nanotubes on rat bone marrow stromal cells Peer-reviewed

Nishida H, Egusa H, Sekino T, Taguchi Y, Komasa S, Kusumoto K, Tanaka M, Yamamoto K

Journal of Japan Association of Oral Rehabilitation　24　(1)　52-57　2011
PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis Peer-reviewed

Mika Inoue, Makio Saeki, Hiroshi Egusa, Hitoshi Niwa, Yoshinori Kamisaki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　403　(3-4)　340-344　2010/12

DOI： 10.1016/j.bbrc.2010.11.031 　

ISSN： 0006-291X
Nuclear Import Mechanism for Myocardin Family Members and Their Correlation with Vascular Smooth Muscle Cell Phenotype Peer-reviewed

Seiji Nakamura, Ken'ichiro Hayashi, Kazuhiro Iwasaki, Tomoaki Fujioka, Hiroshi Egusa, Hirofumi Yatani, Kenji Sobue

JOURNAL OF BIOLOGICAL CHEMISTRY　285　(48)　37314-37323　2010/11

DOI： 10.1074/jbc.M110.180786 　

ISSN： 0021-9258
Gingival Fibroblasts as a Promising Source of Induced Pluripotent Stem Cells Peer-reviewed

Hiroshi Egusa, Keisuke Okita, Hiroki Kayashima, Guannan Yu, Sho Fukuyasu, Makio Saeki, Takuya Matsumoto, Shinya Yamanaka, Hirofumi Yatani

PLOS ONE　5　(9)　e12743　2010/09

DOI： 10.1371/journal.pone.0012743 　

ISSN： 1932-6203
Fabrication of Three-Dimensional Cell Constructs Using Temperature-Responsive Hydrogel Peer-reviewed

Jun-Ichi Sasaki, Taka-Aki Asoh, Takuya Matsumoto, Hiroshi Egusa, Taiji Sohmura, Eben Alsberg, Mitsuru Akashi, Hirofumi Yatani

TISSUE ENGINEERING PART A　16　(8)　2497-2504　2010/08

DOI： 10.1089/ten.tea.2009.0523 　

ISSN： 1937-3341
Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts Peer-reviewed

Shinya Nakamura, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroshi Egusa, Kuen Yong Lee, Takayoshi Nakano, Taiji Sohmura, Atsushi Nakahira

TISSUE ENGINEERING PART A　16　(8)　2467-2473　2010/08

DOI： 10.1089/ten.tea.2009.0337 　

ISSN： 1937-3341
Susceptibility of Candida albicans filamentation-defective mutants to clinical biocides Peer-reviewed

T. Watamoto, L. P. Samaranayake, H. Egusa, H. Yatani, Y. H. Samaranayke, C. J. Seneviratne

JOURNAL OF HOSPITAL INFECTION　74　(2)　189-191　2010/02

DOI： 10.1016/j.jhin.2009.10.017 　

ISSN： 0195-6701
A small-molecule approach to bone regenerative medicine in dentistry Peer-reviewed

Hiroshi Egusa, Makio Saeki, Masanori Doi, Sho Fukuyasu, Takuya Matsumoto, Yoshinori Kamisaki, Hirofumi Yatani

Journal of Oral Biosciences　52　(2)　107-118　2010
Publisher: Japanese Association for Oral Biology
DOI： 10.2330/joralbiosci.52.107 　

ISSN： 1349-0079
In vitro engineering of transitional tissue by patterning and functional control of cells in fibrin gel Peer-reviewed

Jun-Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Takayoshi Nakano, Takuya Ishimoto, Taiji Sohmura, Hirofumi Yatani

SOFT MATTER　6　(8)　1662-1667　2010

DOI： 10.1039/b922418a 　

ISSN： 1744-683X
iPS細胞研究の進展と歯科医学への拡がり Invited

江草宏, 萱島浩輝, 于冠男, 矢谷博文

大阪大学歯学雑誌　55　(1)　11-29　2010
Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans

T. Watamoto, L. P. Samaranayake, J. A. M. S. Jayatilake, H. Egusa, H. Yatani, C. J. Seneviratne

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS　34　(4)　333-339　2009/10

DOI： 10.1016/j.ijantimicag.2009.03.008 　

ISSN： 0924-8579
Mass Fabrication of Small Cell Spheroids by Using Micro-patterned Tissue Culture Plate Peer-reviewed

Akinari Iwasaki, Takuya Matsumoto, Go Tazaki, Hitoshi Tsuruta, Hiroshi Egusa, Hiroyuki Miyajima, Taiji Sohmura

ADVANCED ENGINEERING MATERIALS　11　(10)　801-804　2009/10

DOI： 10.1002/adem.200800392 　

ISSN： 1438-1656
Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts Peer-reviewed

Hiroshi Egusa, Yoshitoshi Kaneda, Yoshihiro Akashi, Yoshinosuke Hamada, Takuya Matsumoto, Makio Saeki, Devang K. Thakor, Yasuhiko Tabata, Nariaki Matsuura, Hirofumi Yatani

BIOMATERIALS　30　(27)　4676-4686　2009/09

DOI： 10.1016/j.biomaterials.2009.05.032 　

ISSN： 0142-9612
Restoration of occlusal and proximal contacts by a single molar crown improves the smoothness of the masticatory movement Peer-reviewed

Takao Watamoto, Hiroshi Equsa, Takahiro Mizumori, Kohtaro Yashiro, Kenji Takada, Hirofumi Yatani

JOURNAL OF DENTISTRY　36　(12)　984-992　2008/12

DOI： 10.1016/j.jdent.2008.08.005 　

ISSN： 0300-5712
Clinical Evaluation of the Efficacy of Removing Microorganisms to Disinfect Patient-Derived Dental Impressions Peer-reviewed

Hiroshi Egusa, Takao Watamoto, Takuya Matsumoto, Keiko Abe, Munemasa Kobayashi, Yoshihiro Akashi, Hirofumi Yatani

INTERNATIONAL JOURNAL OF PROSTHODONTICS　21　(6)　531-538　2008/11

ISSN： 0893-2174
SUSPECTED ASSOCIATION OF AN ALLERGIC REACTION WITH TITANIUM DENTAL IMPLANTS: A CLINICAL REPORT Peer-reviewed

Hiroshi Egusa, Nagakazu Ko, Tsunetoshi Shimazu, Hirofumi Yatani

JOURNAL OF PROSTHETIC DENTISTRY　100　(5)　344-347　2008/11

DOI： 10.1016/S0022-3913(08)60233-4 　

ISSN： 0022-3913
Oral Candidosis in HIV-Infected Patients Peer-reviewed

Hiroshi Egusa, Niroshani S. Soysa, Arjuna N. Ellepola, Hirofumi Yatani, Lakshman P. Samaranayake

CURRENT HIV RESEARCH　6　(6)　485-499　2008/11

DOI： 10.2174/157016208786501445 　

ISSN： 1570-162X

eISSN： 1873-4251
PICK1 binds to calcineurin B and modulates the NFAT activity in PC12 cells Peer-reviewed

Tsutomu Iida, Hiroshi Egusa, Makio Saeki, Hirofumi Yatani, Yoshinori Kamisaki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　375　(4)　655-659　2008/10

DOI： 10.1016/j.bbrc.2008.08.090 　

ISSN： 0006-291X

eISSN： 1090-2104
Molecular cloning of novel Monad binding protein containing tetratricopeptide repeat domains Peer-reviewed

Yuki Itsuki, Makio Saeki, Hirokazu Nakahara, Hiroshi Egusa, Yasuyuki Irie, Yutaka Terao, Shigetada Kawabata, Hirofumi Yatani, Yoshinori Kamisaki

FEBS Letters　582　(16)　2365-2370　2008/07/09

DOI： 10.1016/j.febslet.2008.05.041 　

ISSN： 0014-5793
The influence of single molar crown placement on the smoothness of masticatory movement Peer-reviewed

T. Watamoto, T. Mizumori, H. Egusa, K. Yashiro, K. Takada, H. Yatani

JOURNAL OF ORAL REHABILITATION　35　(6)　440-445　2008/06

DOI： 10.1111/j.1365-2842.2007.01809.x 　

ISSN： 0305-182X
Trabecular bone deterioration in col9a1(+/-) mice associated with enlarged Osteoclasts adhered to collagen IX-deficient bone Peer-reviewed

Chiachien Jake Wang, Keisuke Iida, Hiroshi Egusa, Akishige Hokugo, Anahid Jewett, Ichiro Nishimura

JOURNAL OF BONE AND MINERAL RESEARCH　23　(6)　837-849　2008/06

DOI： 10.1359/JBMR.080214 　

ISSN： 0884-0431
An analysis of the persistent presence of opportunistic pathogens on patient-derived dental impressions and gypsum casts Peer-reviewed

Hiroshi Egusa, Takao Watamoto, Keiko Abe, Munemasa Kobayashi, Yoshitoshi Kaneda, Shunji Ashicla, Takuya Matsumoto, Hirofumi Yatani

INTERNATIONAL JOURNAL OF PROSTHODONTICS　21　(1)　62-68　2008/01

ISSN： 0893-2174
Three-Dimensional Cell and Tissue Patterning in a Strained Fibrin Gel System Peer-reviewed

Takuya Matsumoto, Jun-Ichi Sasaki, Eben Alsberg, Hiroshi Egusa, Hirofumi Yatani, Taiji Sohmura

PLOS ONE　2　(11)　2007/11

DOI： 10.1371/journal.pone.0001211 　

ISSN： 1932-6203
Downregulation of extracellular matrix-related gene clusters during osteogenic differentiation of human bone marrow and adipose tissue-derived stromal cells Peer-reviewed

Hiroshi Egusa, Keisuke Iida, Munemasa Kobayashi, Terry Y. Lin, Min Zhu, Patricia A. Zuk, Chiachien Jake Wang, Devang K. Thakor, Marc H. Hedrick, Ichiro Nishimura

TISSUE ENGINEERING　13　(10)　2589-2600　2007/10

DOI： 10.1089/ten.2007.0080 　

ISSN： 1076-3279
Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold bioniaterials Peer-reviewed

Yoshinosuke Hamada, Hiroshi Egusa, Yoshitoshi Kaneda, Isao Hirata, Naomasa Kawaguchi, Takafumi Hirao, Takuya Matsumoto, Makiko Yao, Kiyoshi Daito, Mayuka Suzuki

DENTAL MATERIALS JOURNAL　26　(4)　487-492　2007/07

DOI： 10.4012/dmj.26.487 　

ISSN： 0287-4547
Modification of apatite materials for bone tissue engineering and drug delivery carriers Peer-reviewed

T. Matsumoto, M. Okazaki, A. Nakahira, J. Sasaki, H. Egusa, T. Sohmura

CURRENT MEDICINAL CHEMISTRY　14　(25)　2726-2733　2007

DOI： 10.2174/092986707782023208 　

ISSN： 0929-8673
The effect of saliva or serum on bacterial and Candida albicans colonization on type I collagen Peer-reviewed

H. Nikawa, H. Egusa, H. Yamashiro, M. Nishimura, S. Makihira, C. Jin, H. Fukushima, T. Hamada

JOURNAL OF ORAL REHABILITATION　33　(10)　767-774　2006/10

DOI： 10.1046/j.0305-182X.2003.01149.x 　

ISSN： 0305-182X
In vitro mechanisms of interleukin-8-mediated responses of human gingival epithelial cells to Candida albicans infection Peer-reviewed

Hiroshi Egusa, Hiroki Nikawa, Seicho Makihira, Hirofumi Yatani, Taizo Hamada

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY　296　(4-5)　301-311　2006/08

DOI： 10.1016/j.ijmm.2005.12.017 　

ISSN： 1438-4221
An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells Peer-reviewed

H Nikawa, H Egusa, S Makihira, T Okamoto, H Kurihara, H Shiba, H Amano, T Murayama, H Yatani, T Hamada

MYCOSES　49　(1)　14-17　2006/01

DOI： 10.1111/j.1439-0507.2005.01176.x 　

ISSN： 0933-7407
Neuronal differentiation of bone marrow-derived stromal stem cells involves suppression of discordant phenotypes through gene silencing Peer-reviewed

H Egusa, FE Schweizer, CC Wang, Y Matsuka, Nishimura, I

JOURNAL OF BIOLOGICAL CHEMISTRY　280　(25)　23691-23697　2005/06

DOI： 10.1074/jbc.M413796200 　

ISSN： 0021-9258
2 Methodology of microarray data analysis

Zhou, X., Egusa, H., Cole, S.W., Nishimura, I., Wong, D.T.W.

Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas　3　2005

DOI： 10.1016/S1874-5784(05)80008-X 　

ISSN： 1874-5784
Intercellular adhesion molecule 1-dependent activation of interleukin 8 expression in Candida albicans-infected human gingival epithelial cells Peer-reviewed

H Egusa, H Nikawa, S Makihira, A Jewett, H Yatani, T Hamada

INFECTION AND IMMUNITY　73　(1)　622-626　2005/01

DOI： 10.1128/IAI.73.1.622-626.2005 　

ISSN： 0019-9567
Candida adherence and biofilm formation on oral surfaces

Hiroki Nikawa, Seicho Mikihira, Hiroshi Egusa, Hitoshi Fukushima, Ryoko Kawabata, Taizo Hamada, Hirohumi Yatani

Japanese Journal of Medical Mycology　46　(4)　233-242　2005
Publisher: Japanese Society for Medical Mycology
DOI： 10.3314/jjmm.46.233 　

ISSN： 0916-4804
In vitro cariogenic potential of Candida albicans Peer-reviewed

H Nikawa, H Yamashiro, S Makihira, M Nishimura, H Egusa, M Furukawa, D Setijanto, T Hamada

MYCOSES　46　(11-12)　471-478　2003/12

DOI： 10.1046/j.0933-7407.2003.00888.x 　

ISSN： 0933-7407
A novel technique to evaluate the adhesion of Candida species to gingival epithelial cells Peer-reviewed

H Nikawa, H Egusa, S Makihira, M Nishimura, K Ishida, M Furukawa, T Hamada

MYCOSES　46　(9-10)　384-389　2003/10

DOI： 10.1046/j.0933-7407.2003.00912.x 　

ISSN： 0933-7407
Biofilm formation of Candida albicans on the surfaces of deteriorated soft denture lining materials caused by denture cleansers in vitro Peer-reviewed

H Nikawa, C Jin, S Makihira, H Egusa, T Hamada, H Kumagai

JOURNAL OF ORAL REHABILITATION　30　(3)　243-250　2003/03

DOI： 10.1046/j.1365-2842.2003.01024.x 　

ISSN： 0305-182X
Impact of components of denture acrylic resin on gingival cell growth and sensitivity to Candida albicans adhesion Peer-reviewed

S Makihira, H Nikawa, M Nishimura, H Egusa, S Sadamori, RP Rahayu, H Nishimura, T Hamada

MYCOSES　45　(8)　300-305　2002/10

DOI： 10.1046/j.1439-0507.2002.00769.x 　

ISSN： 0933-7407
Alteration of the coadherence of Candida albicans with oral bacteria by dietary sugars Peer-reviewed

H Nikawa, H Egusa, S Makihira, H Yamashiro, H Fukushima, C Jin, M Nishimura, RR Pudji, T Hamada

ORAL MICROBIOLOGY AND IMMUNOLOGY　16　(5)　279-283　2001/10

DOI： 10.1034/j.1399-302X.2001.016005279.x 　

ISSN： 0902-0055
Potent role of histatin in natural defense system against oral candidiasis Peer-reviewed

Surabaya Dental Journal　34　47-51,　2001/04/01
Integrin and cancer metastasis Peer-reviewed

Surabaya Dental Journal　34　32-34　2001/04/01
Effects of Eluates of Denture Adhesives on Human Gingival Fibroblasts

MAKIHIRA Seicho, NIKAWA Hiroki, NISHIMURA Haruki, NISHIMURA Masahiro, MURATA Hiroshi, SADAMORI Shinsuke, ISHIDA Kazuhiro, YAMASHIRO Hirofumi, JIN Chen, EGUSA Hiroshi, FUKUSHIMA Hitoshi, HAMADA Taizo

Journal of Prosthodontic Research　45　(3)　403-411　2001
Publisher: Japan Prosthodontic Society
DOI： 10.2186/jjps.45.403 　

ISSN： 0389-5386 1883-177X

More details Close

Purpose: The purpose of this study was to evaluate biological effects (i. e. cytotoxicity and interleukin 1β production) of eluates from commercially available denture adhesives (7 cushion-types, 5 creamtypes, 1 powder-type, and 2 seal-types) on human gingival fibroblasts. Materials and Methods: To achieve this purpose, defined amounts of commercially available denture adhesives were dialyzed (cut-off MW: 12, 000 to 14, 000) against Dulbecco's Modified Eagle Medium for one or two days. The cytotoxicity and interleukin 1β inductions of these eluates were analyzed by aqueous soluble tetrazolium/formazan assay (MTS assay) and enzyme-linked immunosorbent assay (ELISA), respectively. Results: The cytotoxicity against human fibroblast cells of the materials was varied, to a lesser or greater extent, depending on the type of materials. Particularly, cream-type denture adhesives caused much population of cell death, whereas cushion-type materials showed neither cytotoxicity nor induction of interleukin 1β. Though several materials acidified the dialysates, such a pH change was suggested to have no effects on cell viability. Conclusion: The results of this study implied that several commercially availfable products of denture adhesives may be cytotoxic to human oral tissues in vivo.
Exposure to subtherapeutic concentrations of polyene antifungals suppresses the adherence of Candida species to denture acrylic Peer-reviewed

H Egusa, ANB Ellepola, H Nikawa, T Hamada, LP Samaranayake

CHEMOTHERAPY　46　(4)　267-274　2000/07

DOI： 10.1159/000007298 　

ISSN： 0009-3157
Sub-therapeutic exposure to polyene antimycotics elicits a post-antifungal effect (PAFE) and depresses the cell surface hydrophobicity of oral Candida albicans isolates Peer-reviewed

H Egusa, ANB Ellepola, H Nikawa, T Hamada, LP Samaranayake

JOURNAL OF ORAL PATHOLOGY & MEDICINE　29　(5)　206-213　2000/05

DOI： 10.1034/j.1600-0714.2000.290503.x 　

ISSN： 0904-2512

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Misc. 131

筋萎縮性側索硬化症患者に対する舌接触補助床の効果の検討

互野亮, 白石成, 江草宏, 小山重人

Japanese Journal of Maxillo Facial Implants　23　(3)　223-223　2024/11
Publisher: (公社)日本顎顔面インプラント学会
ISSN： 1347-894X

eISSN： 2433-5509
歯を切削する技能を可視化/スコア化した評価システムの開発

遠藤弥生, 勝田悠介, 互野亮, 尾崎茜, 山田将博, 江草宏, 董哲耀, 熊澤逸夫

生体医歯工学共同研究拠点成果報告書　2023年度　185-185　2024/04
Publisher: 東京医科歯科大学生体材料工学研究所
Functional training methods that activates oral and cognitive functions: Effect verification through double-blind RCT

阿部真澄, 野内類, 小川徹, 白石成, 日原大貴, 豊岡継泰, 土井千章, 檜山聡, 佐々木啓一, 江草宏, 依田信裕

日本補綴歯科学会誌(Web)　16　2024

ISSN： 1883-6860
超音波スケーラーによる飛沫エアロゾルの可視化と吸引装置による拡散抑制効果

渡辺隼, 小林洋子, 江草宏

日本口腔内科学会雑誌　29　(2)　89-89　2023/12
Publisher: (一社)日本口腔内科学会
ISSN： 2186-6147

eISSN： 2186-6155
歯科訪問診療用ポータブルユニットの水質管理に対する影響要因の検討

伏見国拡, 渡辺隼, 山田将博, 江草宏

日本口腔内科学会雑誌　29　(2)　90-90　2023/12
Publisher: (一社)日本口腔内科学会
ISSN： 2186-6147

eISSN： 2186-6155
東北大学病院歯科部門における過去16年間の誤飲・誤嚥インシデント

小林洋子, 倉内美智子, 渡辺隼, 草間太郎, 菅原寛子, 西條文人, 田畑雅央, 飯久保正弘, 杉浦剛, 江草宏

医療の質・安全学会誌　18　(Suppl.)　352-352　2023/11
Publisher: (一社)医療の質・安全学会
ISSN： 1881-3658

eISSN： 1882-3254
ミクロ/ナノ階層的表面改質によるチタンインプラントの骨結合強さの増強

鹿内陽樹, 山田将博, 大竹孝幸, 渡辺隼, 江草宏

日本補綴歯科学会誌　15　(東北・北海道支部学術大会特別号)　37-37　2023/10
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
大学病院歯科インプラントセンターにおける初診患者の実態調査

尾崎茜, 依田信裕, 山内健介, 新部邦透, 森島浩允, 庄原健太, 小山重人, 江草宏

日本口腔インプラント学会誌　36　(特別号)　P-2　2023/09
Publisher: (公社)日本口腔インプラント学会
ISSN： 0914-6695

eISSN： 2187-9117
Greetings from Editor-in-Chief Invited Peer-reviewed

Egusa H

J Prosthodont Res　67　(3)　vi-vii　2023/07
歯科訪問診療における水質管理方策の検討

伏見国拡, 山田将博, 渡辺隼, 鷲尾純平, 高橋信博, 江草宏

東北大学歯学雑誌　41/42　(2/1)　45-45　2023/06
Publisher: 東北大学歯学会
ISSN： 0287-3915
歯科用エアータービンにより発生する飛沫エアロゾル挙動の可視化と吸引装置の有効性

渡辺隼, 小林洋子, 江草宏

日本補綴歯科学会誌　15　(特別号)　241-241　2023/05
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
歯根膜応用を目指した石灰化dECM膜のin vivo骨結合性評価

鈴木美加, 木村剛, 岡田正弘, 松本卓也, 山田将博, 江草宏, 中村奈緒子, 岸田晶夫

日本歯科理工学会誌　42　(Special Issue81)　18-18　2023/03
Publisher: (一社)日本歯科理工学会
ISSN： 1884-4421
In vivo bone affinity evaluation of mineralized dECM membrane for application as hard-soft interregional tissue.

鈴木美加, 木村剛, 橋本良秀, 岡田正弘, 松本卓也, 高橋宏信, 清水達也, 山田将博, 江草宏, 中村奈緒子, 岸田晶夫

日本バイオマテリアル学会大会予稿集(Web)　45th　2023
Verification of brain activity during cognitive and oral functional training in young adults

阿部真澄, 野内類, 依田信裕, 小川徹, 白石成, 日原大貴, 佐々木啓一, 江草宏

日本補綴歯科学会誌(Web)　15　2023

ISSN： 1883-6860
東北大学病院歯科部門における過去15年間のインシデントレポート分析

小林洋子, 倉内美智子, 渡辺準, 草間太郎, 遠藤英昭, 菅原寛子, 西條文人, 田畑雅央, 飯久保正弘, 江草宏

医療の質・安全学会誌　17　(Suppl.)　290-290　2022/11
Publisher: (一社)医療の質・安全学会
ISSN： 1881-3658

eISSN： 1882-3254
歯根膜応用を目指した骨親和性を有するdECM膜の調製

鈴木美加, 木村剛, 橋本良秀, 岡田正弘, 松本卓也, 高橋宏信, 清水達也, 山田将博, 江草宏, 中村奈緒子, 岸田晶夫

日本バイオマテリアル学会大会予稿集　44回　251-251　2022/11
Publisher: 日本バイオマテリアル学会
歯科治療で発生するエアロゾル挙動の可視化

小林洋子, 渡辺隼, 梶田倫功, 山内健介, 金高弘恭, 江草宏, 五十嵐薫, 矢代航, 菊地謙次, 西村秀一, 森島浩允, 佐藤舜, 菅井優生

東北大学歯学雑誌　39/40/41　(2/1-2/1)　49-49　2022/06
Publisher: 東北大学歯学会
ISSN： 0287-3915
新型コロナウイルス感染症(COVID-19)感染拡大に伴う東北大学病院歯科診療部門のオンライン診療の現状

菅原由美子, 佐藤しづ子, 野上晋之介, 千葉雅俊, 庄司憲明, 高橋哲, 江草宏, 佐々木啓一, 五十嵐薫, 飯久保正弘

東北大学歯学雑誌　39/40/41　(2/1-2/1)　19-25　2022/06
Publisher: 東北大学歯学会
ISSN： 0287-3915
歯科治療で発生するエアロゾル挙動の可視化

小林洋子, 渡辺隼, 梶田倫功, 山内健介, 金高弘恭, 江草宏, 五十嵐薫, 矢代航, 菊地謙次, 西村秀一, 森島浩允, 佐藤舜, 菅井優生

東北大学歯学雑誌　39/40/41　(2/1-2/1)　49-49　2022/06
Publisher: 東北大学歯学会
ISSN： 0287-3915
Periodonatal tissue regeneration using decellularized tissue

木村剛, 中村奈緒子, 中村奈緒子, 山田将博, 岡田正弘, 江草宏, 松本卓也, 岸田晶夫

日本再生医療学会総会(Web)　21st　2022
カテキンの光酸化反応機構の解明および活性酸素を介した殺菌活性評価

宍戸駿一, 宮野怜, 中島琢自, 松尾洋孝, 岩月正人, 中村圭祐, 菅野太郎, 江草宏, 庭野吉己, 庭野吉己

日本ポリフェノール学会学術集会プログラム・講演抄録集　15th　2022
訪問歯科診療用ポータブルユニット排水における遊離残留塩素濃度の検討

伏見国拡, 渡辺隼, 山田将博, 江草宏

日本補綴歯科学会誌　13　(東北・北海道支部学術大会特別号)　27-27　2021/10
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
生体内微小環境を再現したバイオマテリアルによるin situ歯周組織再生

山田将博, 木村剛, 中村奈緒子, 吉岡勇人, 新野秀憲, 江草宏, 江草宏

日本再生医療学会総会(Web)　20th　2021
Development of a bone regeneration technology focusing on iPS cell spheroids size

巽秀人, 盛林昭仁, 萱島浩輝, 石垣尚一, 江草宏, 江草宏, 矢谷博文

日本補綴歯科学会誌(Web)　13　2021

ISSN： 1883-6860
骨再生技術の発展を目指したiPS細胞スフェロイドの至適サイズの探索

巽秀人, 盛林昭仁, 山本治毅, 萱島浩輝, 三浦治郎, 石垣尚一, 江草宏, 矢谷博文

日本口腔インプラント学会誌　33　(特別号)　253-253　2020/09
Publisher: (公社)日本口腔インプラント学会
ISSN： 0914-6695

eISSN： 2187-9117
大臼歯CAD/CAM冠治療に関する後ろ向きコホート研究

猪股実祐, 原田章生, 笠原紳, 勝田悠介, 尾崎茜, 木村葉月, 江草宏

日本補綴歯科学会誌　12　(特別号)　175-175　2020/06
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
咀嚼および審美障害を訴える患者に対して包括的歯科治療で咬合再構成した症例

唐木俊英, 原田章生, 山田将博, 江草宏

日本補綴歯科学会誌　12　(特別号)　269-269　2020/06
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
大臼歯CAD/CAM冠治療に関する後ろ向きコホート研究

猪股実祐, 原田章生, 笠原紳, 勝田悠介, 尾崎茜, 木村葉月, 江草宏

日本補綴歯科学会誌　12　(特別号)　175-175　2020/06
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
小臼歯CAD/CAM冠用レジンブロックで作製した大臼歯クラウンの40症例

峯篤史, 大野彩, 黒崎陽子, 三野卓哉, 伴晋太朗, 腰原輝純, 細木真紀, 原田章生, 江草宏, 松香芳三, 佐藤亨, 窪木拓男, 矢谷博文

日本補綴歯科学会誌　12　(特別号)　266-266　2020/06
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
咀嚼および審美障害を訴える患者に対して包括的歯科治療で咬合再構成した症例

唐木俊英, 原田章生, 山田将博, 江草宏

日本補綴歯科学会誌　12　(特別号)　269-269　2020/06
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
レドックスバイオロジーを基盤とした骨再生技術の創成

渡辺隼, 山田将博, 江草宏

日本歯科医学会誌　39　57-57　2020/03
Publisher: 日本歯科医学会
ISSN： 0286-164X
CAD/CAM教育システムが次代の歯科医療を切り開く～東北大学歯学部の挑戦～

GC Circle　172　32-35　2020/01
One-year prospective study on 40 molar crowns fabricated from premolar CAD/CAM resin block.

峯篤史, 大野彩, 黒崎陽子, 三野卓哉, 伴晋太朗, 腰原輝純, 細木真紀, 原田章生, 江草宏, 松香芳三, 佐藤亨, 窪木拓男, 矢谷博文

日本補綴歯科学会誌(Web)　12　2020

ISSN： 1883-6860
マクロファージの細菌性炎症応答制御におけるLipin2の役割

綿引麻美, 清水康平, 星川聖良, 千葉満生, 福本敏, 江草宏, 犬塚博之

Journal of Oral Biosciences Supplement　2019　131-131　2019/10
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
災害歯科医療学実地研修会の参加者に対するアンケート調査

小林洋子, 西岡貴志, 冨士岳志, 齋藤恵一, 洪光, 江草宏, 佐々木啓一

第38回日本歯科医学教育学会総会および学術大会　38th　2019/07
骨髄間葉系幹細胞のNAC処理は抗アポトーシスおよび抗炎症作用を介して骨再生を促す

渡辺隼, 山田将博, 江草宏

日本補綴歯科学会誌　11　(特別号)　131-131　2019/05
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
東北大学歯学部における4年次CAD/CAM冠実習が支台歯形成技能に及ぼす教育効果

勝田悠介, 奥山弘生, 石橋実, 山田将博, 江草宏

日本補綴歯科学会誌　11　(特別号)　327-327　2019/05
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
CAD/CAM冠を介した光照射がデュアルキュア型レジンセメントの接着に及ぼす影響

尾崎茜, 原田章生, 勝田悠介, 木村葉月, 江草宏

日本補綴歯科学会誌　11　(特別号)　233-233　2019/05
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
歯周組織を誘導する生体模倣チタンナノ表面インプラントの開発

山田将博, 江草宏, 新野秀憲, 吉岡勇人, 木村剛, 中村奈緒子

生体医歯工学共同研究拠点成果報告書　平成30年度　51-51　2019/04
Publisher: 東京医科歯科大学生体材料工学研究所
低温劣化処理および超音波スケーリングがジルコニアの表面性状に与える影響

中澤晃介, 原田章生, 江草宏

日本歯科理工学会誌　37　(Special Issue71)　39-39　2018/03
Publisher: (一社)日本歯科理工学会
ISSN： 1884-4421
カテキンの光酸化を介した殺菌活性と光酸化機序の解明-その2-

宍戸駿一, 宮野怜, 中島琢自, 松尾洋孝, 岩月正人, 中村圭祐, 菅野太郎, 江草宏, 庭野吉己

日本防菌防黴学会年次大会要旨集　45th　2018
CAD/CAM 冠の臨床経過～トラブルの原因と適応症例を考える～ Invited

三浦賞子, 江草宏

歯界展望　132　(1)　28-35　2018
アミロライドがオクタアルギニン/リン酸カルシウムを用いた遺伝子導入に及ぼす影響(Amiloride-enhanced gene transfection of octa-arginine functionalized calcium phosphate nanoparticles)

バネガス・サネンズ・フアンラモン, 天雲太一, 鎌野優弥, 江草宏, 小川徹, 佐々木啓一

日本補綴歯科学会誌　9　(特別号)　276-276　2017/06
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
小臼歯CAD/CAM冠の適応症に関する後ろ向きコホート研究

三浦賞子, 笠原紳, 山内しのぶ, 勝田悠介, 原田章生, 江草宏

日本補綴歯科学会誌　9　(特別号)　123-123　2017/06
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
ヒトiPS細胞に低酸素培養を応用した再生医療用骨組織の作製

大川博子, 江草宏

日本歯科医学会誌　36　60-60　2017/03
Publisher: 日本歯科医学会
ISSN： 0286-164X
カテキンの光酸化を介した殺菌活性と光酸化機序の解明

宍戸駿一, 宮野怜, 中島琢自, 中島琢自, 佐藤恵美子, 中村圭祐, 菅野太郎, 江草宏, 庭野吉己

日本防菌防黴学会年次大会要旨集　44th　2017
小臼歯CAD/CAM冠の適応症に関する後ろ向きコホート研究

三浦賞子, 笠原紳, 山内しのぶ, 勝田悠介, 原田章生, 江草宏

日本補綴歯科学会誌(Web)　9　123 (WEB ONLY)　2017

ISSN： 1883-6860
Gene delivery and expression systems in induced pluripotent stem cells Invited

Zhang M, Niibe K, Kondo T, Kamano Y, Saeki M, Egusa H

Interface Oral Health Science 2016, Springer　121-133　2017
CAD/CAM冠用コンポジットレジンの疲労動態解析

安久修平, 原田章生, 菅野太郎, 江草宏

日本補綴歯科学会誌　8　(特別号)　245-245　2016/07
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
CAD/CAM冠用コンポジットレジンの疲労動態解析

安久修平, 原田章生, 菅野太郎, 江草宏

日本補綴歯科学会誌(Web)　8　245 (WEB ONLY)　2016/07

ISSN： 1883-6860
身近な臨床・これからの歯科医のための臨床講座 82 インプラント上部構造に何を選びますか?~知っておきたい歯冠修復材料の特徴~

原田章生, 山田将博, 江草宏

日本歯科医師会雑誌　69　(1)　41‐47,1-47,1　2016/04/10
Publisher: 日本歯科医師会
ISSN： 0047-1763
iPS細胞由来の再生因子を用いた唾液腺再生の検討

小野瞳, 尾花綾, 宇佐美悠, 酒井学, 井階一樹, 三浦治郎, 江草宏, 阪井丘芳

日本口腔科学会雑誌　65　(1)　62-62　2016/03
Publisher: (NPO)日本口腔科学会
ISSN： 0029-0297
iPS細胞由来の再生因子を用いた唾液腺再生の検討

小野瞳, 尾花綾, 宇佐美悠, 酒井学, 井階一樹, 三浦治郎, 江草宏, 阪井丘芳

日本口腔科学会雑誌(Web)　65　(1)　2016

ISSN： 2185-0461
破骨細胞の分化を誘導する小分子化合物の探索

西川実沙, 柿原嘉人, 秋葉陽介, 江草宏, 佐伯万騎男

Journal of Oral Biosciences Supplement　2015　546-546　2015/09
Publisher: (一社)歯科基礎医学会
ISSN： 2187-2333

eISSN： 2187-9109
iPS細胞を応用した唾液腺再生モデル

小野瞳, 尾花綾, 宇佐美悠, 酒井学, 三浦治郎, 江草宏, 阪井丘芳

日本口腔科学会雑誌　64　(2)　204-204　2015/07
Publisher: (NPO)日本口腔科学会
ISSN： 0029-0297
臨床シミュレーション実習におけるComputer Graphicsを応用した支台歯形成トレーニングの試み

奥山弥生, 石橋実, 服部佳功, 佐々木啓一, 江草宏

日本歯科医学教育学会総会・学術大会プログラム・抄録集　34th　92-92　2015/07
Publisher: 日本歯科医学教育学会
The effect of CDK inhibitors on osteoclast differentiation Peer-reviewed

Yoshito Kakihara, Juri Nakata, Yosuke Akiba, Hiroshi Egusa, Makio Saeki

JOURNAL OF PHARMACOLOGICAL SCIENCES　128　(3)　S242-S242　2015/07

ISSN： 1347-8613

eISSN： 1347-8648
破骨細胞分化に影響を与える薬剤の探索

中田樹里, 柿原嘉人, 丹原惇, 齋藤功, 江草宏, 佐伯万騎男

日本骨代謝学会学術集会プログラム抄録集　33回　196-196　2015/07
Publisher: (一社)日本骨代謝学会
ISSN： 1349-0761
過酸化水素光分解殺菌技術における光の波長と過酸化水素濃度の相乗作用

唐木俊英, 倉内美智子, 菅野太郎, 勝田悠介, 猪飼紘代, 林栄成, 佐々木啓一, 江草宏

日本補綴歯科学会誌　7　(特別号)　154-154　2015/05
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426

eISSN： 1883-6860
過酸化水素光分解殺菌技術における光の波長と過酸化水素濃度の相乗作用

唐木俊英, 倉内美智子, 菅野太郎, 勝田悠介, 猪飼紘代, 林栄成, 佐々木啓一, 江草宏

日本補綴歯科学会誌(Web)　7　2015

ISSN： 1883-6860
破骨細胞の分化を誘導する小分子化合物の探索

西川実沙, 柿原嘉人, 秋葉陽介, 江草宏, 佐伯万騎男

Journal of Oral Biosciences Supplement (Web)　2015　2015

ISSN： 2187-9109
破骨細胞分化に影響を与える薬剤のケミカルライブラリースクリーニング

中田樹里, 中田樹里, 柿原嘉人, 秋葉陽介, 丹原惇, 齋藤功, 江草宏, 佐伯万騎男

日本薬理学会北部会プログラム・抄録集　66th　53　2015
Feeder cell sources and feeder-free methods for human ips cell culture Invited

Guannan Yu, Fangfang Wang, Hiroko Okawa, Hirofumi Yatani

Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent Interface　2014　145-159　2015/01/01
Publisher: Springer Japan
DOI： 10.1007/978-4-431-55192-8_12 　
iPS細胞を用いた唾液腺再生への試み

小野瞳, 尾花綾, 宇佐美悠, 江草宏, 大川博子, 山城隆, 野原幹司, 阪井丘芳

日本口腔科学会雑誌　63　(4)　393-393　2014/09
Publisher: (NPO)日本口腔科学会
ISSN： 0029-0297

eISSN： 2185-0461
Development of safety evaluation tools for oral care reagents using gingiva-derived iPS cells

22　78-83　2014
Publisher: コスメトロジー研究振興財団
ISSN： 2188-563X
小分子化合物を応用した感染応答性義歯床材料の開発

綿本隆生, 江草宏, 矢谷博文, 澤瀬隆

日本補綴歯科学会誌　5　(特別号)　114-114　2013/05
Publisher: (公社)日本補綴歯科学会
ISSN： 1883-4426
mTOR結合タンパク質PIH1D1がrRNA転写に及ぼす影響

外園真規, 佐伯万騎男, 鎌野優弥, 江草宏, 上崎善規

日本薬理学雑誌　141　(3)　30P-30P　2013/03
Publisher: (公社)日本薬理学会
ISSN： 0015-5691

eISSN： 1347-8397
iPS細胞と遺伝子診断で進化する再生医療～最先端のPersonalized Dentistry Invited

江草宏, 西村一郎

GC Circle　142　4-11　2012/08
Development of endochondral ossification analysis tool using 3D cell constructs

SASAKI JI, MATSUMOTO T, EGUSA H, YATANI H, IMAZATO S

The journal of the Japanese Society for Dental Materials and Devices　31　(2)　138-138　2012/03/25
Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)
ISSN： 1884-4421

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We previously reported that three-dimensional (3D) mesenchymal stem cells (MSCs) construct could be fabricated by using thermo-responsive hydrogel. In this study, internal/external environments of the 3D cell construct induced by osteoblastic differentiation were investigated. The specific regions with chondrogenesis and osteogenesis were detected in the 3D cell construct during the culture. Calcified matrix was formed from MSCs inthe process of endochondral ossification. The 3D cell construct composed of MSCs could be a promising toolfor in vitro investigation of osteogenesis.
The impact of gingival fibroblast-derived iPS cells in dentistry Invited

Hiroshi Egusa

Interface Oral Health Science 2011　2011　9-13　2012/01/01
Publisher: Springer Japan
DOI： 10.1007/978-4-431-54070-0_2 　
新規骨移植材料の開発を目指した歯肉由来iPS細胞の骨芽細胞分化誘導法の確立

萱島浩輝, 江草宏, 佐々木淳一, 中野環, 矢谷博文

日本口腔インプラント学会誌　24　(特別号)　142-142　2011/08/25
Publisher: (公社)日本口腔インプラント学会
ISSN： 0914-6695
骨形成促進作用を有する小分子化合物の探索システムの構築

福安翔, 江草宏, 萱島浩輝, 中野環, 矢谷博文

日本口腔インプラント学会誌　24　(特別号)　275-275　2011/08/25
Publisher: (公社)日本口腔インプラント学会
ISSN： 0914-6695
Monadはヒト乳がん細胞MDA-MB-231細胞の浸潤能を負に制御する

成清綾, 吉田好紀, 佐伯万騎男, 井上美香, 島田華奈, 江草宏, 丹羽均, 由良義明, 矢谷博文, 上崎善規

日本薬理学雑誌　137　(3)　30P-30P　2011/03
Publisher: (公社)日本薬理学会
ISSN： 0015-5691

eISSN： 1347-8397
歯肉組織由来線維芽細胞は有用なiPS細胞源である

江草宏, 沖田圭介, 山中伸弥, 矢谷博文

再生医療　10　198　2011/02/01

ISSN： 1347-7919
歯肉をiPS細胞源とする歯科医療技術の創成 Invited

江草宏, 矢谷博文

日本歯科評論　71　(7)　9-11　2011
Publisher: ヒョーロン・パブリッシャーズ
ISSN： 0289-0909
Gingiva-derived iPS cells: A promising tool for dental applications Invited

Egusa H

Proceedings of 4th Hiroshima Conference on Education and Science in Dentistry　32-36　2011
Fabrication of three-dimensional cell constructs

MATSUMOTO T, SASAKI J, EGUSA H, YATANI H

The journal of the Japanese Society for Dental Materials and Devices　29　(5)　382-382　2010/09/20
Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)
ISSN： 1884-4421

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In tissue regeneration therapy, scaffold materials play pivotal roles on carrying cells at the site of tissue regeneration. However, the remained scaffold materials might attenuate the accurate tissue regeneration in some cases. Therefore, creating another cell carrying method without using scaffold material would be important. To obtain scaffold free-3D cell constructs, we synthesized thermo-responsive hydrogel by using poly N-isopropylacrylamide with several cross-linking agents. Consequently, we fabricated clear hydrogel, which changes its volume depending on the temperature. The gel allowed us to obtain 3D cell constructs with desired morphology.
Application of Cyclic Strain to Promote Skeletal Myogenesis of Bone Marrow-Derived Stromal Stem Cells : For Future Use in Tongue and Masticatory Muscle Tissue Engineering

KOBAYASHI M, EGUSA H, AKASHI Y, KAYASHIMA H, YATANI H

1　(118)　83-83　2010/08/16
Synthesis by Chemical Processing and Biocompatibility of Titania Nanotubes

NISHIDA H, EGUSA H, MIURA J, NAKAMURA T, YAMAMOTO K, YATANI H

1　(118)　194-194　2010/08/16
Involvement of DNA Methylation during Osteogenic Differentiation of Mouse Bone Marrow-Derived Stromal Stem Cells

ASHIDA S, EGUSA H, KOBAYASHI M, AKASHI Y, KAYASHIMA H, YATANI H

1　(118)　227-227　2010/08/16
Monad binding protein-2 interacts with RPAP3 and regulates doxorubicin-induced apoptosis in U2OS cell

Mika Inoue, Kana Horiuchi, Makio Saeki, Hiroshi Egusa, Hitoshi Niwa, Yoshinori Kamisaki

JOURNAL OF PHARMACOLOGICAL SCIENCES　112　58P-58P　2010

ISSN： 1347-8613
RPAP3 promotes doxorubicin-induced apoptosis in human breast cancer T-47D cells

Kana Horiuchi, Mika Inoue, Makio Saeki, Hiroshi Egusa, Yoshiaki Yura, Yoshinori Kamisaki

JOURNAL OF PHARMACOLOGICAL SCIENCES　112　113P-113P　2010

ISSN： 1347-8613
iPS細胞が歯科医療に及ぼすインパクト―歯肉から顎骨や歯の再生が可能に？ Invited

江草宏, 萱島浩輝, 矢谷博文

Dental Diamond　35　(16)　60-64　2010
Direct synthesis and biocompatibility of titania nanotube layers on metal substrates

NISHIDA H, EGUSA H, NAKAMURA T, YAMAMOTO K, YATANI H

Journal of the Japanese Society for Dental Materials and Devices　28　(5)　212-212　2009/09/10
Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)
ISSN： 0286-5858

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Titania nanotube is considered to be of particular significance for good biocompatibility and high strength. In this work, we describe new dental material for implant was developed by titania nanotubes(TNTs). Titania nanotubes were prepared by chemical processing on Ti surface. And rat mesenchymal stem cells(rMSC) are cultured on them. A large quantity of calcium was detected on TNT layers. The result may suggest the possibility that TNTs promotes the generation of the bone.
Candida albicansの菌糸およびバイオフィルム形成が抗真菌剤感受性に及ぼす影響

綿本隆生, Seneviratne CJ, 江草宏, Samaranayake LP, 矢谷博文

Bacterial Adherence & Biofilm　23　51-55　2009
DNA Methylation Analysis of Osteogenic Gene Promoter Regions in Mouse Bone Marrow Stromal Cells.

H. Egusa, S. Ashida, M. Kobayashi, Y. Akashi, H. Yatani

JOURNAL OF BONE AND MINERAL RESEARCH　23　S382-S382　2008/09

ISSN： 0884-0431
Effects of Functional Peptide SVVYGLR on Bone Tissue Regeneration

KANEDA Y, EGUSA H, HAMADA Y, YATANI H

52　(117)　131-131　2008/06/06
Functional control of myoblasts in a strained fibrin gel

T. Matsumoto, J. Sasaki, M. H. Uddin, H. Egusa, T. Sohmura

TISSUE ENGINEERING PART A　14　(5)　737-737　2008/05

ISSN： 1937-3341
P-5 Effect of mechanical stress on HSC niche related gene expressions in osteoblasts

SASAKI J., MATSUMOTO T., EGUSA H., SOHMURA T., YATANI H.

Journal of the Japanese Society for Dental Materials and Devices　27　(2)　140-140　2008/03/31
Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)
ISSN： 0286-5858
Involvement of calcineurin in osteoclastogenesis mediated by RANKL

Tsutomu Iida, Hiroshi Egusa, Makio Saeki, Masanori Doi, Hirofumi Yatani, Yoshinori Kamisaki

JOURNAL OF PHARMACOLOGICAL SCIENCES　106　154P-154P　2008

ISSN： 1347-8613
セメンテーション，トライアルセメント：審美歯科・インプラントワードブック

江草宏, 矢谷博文

補綴臨床別冊　92-93　2008
印象体の消毒をどうしていますか？

江草宏, 綿本隆生, 阿部桂子, 矢谷博文

歯界展望　112　(2)　341-347　2008
Current Developments in Oromaxillofacial Regeneration. In Cutting-Edge Biodentistry (Ed. Yoneda T)

Osaka University Press　2008
Cementation and trial cement. In Word Book for Esthetic and Implant Dentistry

Practice in Prosthodontics, Sp. Iss　92-93　2008
P10. Fabrication of 3-dimensional bone matrix alignment(Poster Presentation,The 5^<th> Annual Meeting of Japanese Association of Regeneration Dentistry)

Sasaki Jun-Ichi, Matsumoto Takuya, Egusa Hiroshi, Sohmura Taiji, Yatani Hirofumi

Journal of the Japanese Association of Regenerative Dentistry　5　(1)　58-58　2007/12/30
Publisher: Japanese Association of Regenerative Dentistry
ISSN： 1348-9615
Disinfection of Patients-Derived Dental Impressions Contaminated with Pathogenic Microorganisms

WATAMOTO T, EGUSA H, ABE K, YATANI H

51　(116)　92-92　2007/05/18
The role of calcineurin in osteoclastogenesis mediated by RANKL

Tsutomu Iida, Hiroshi Egusa, Makio Saeki, Masanori Doi, Yuki Itsuki, Hirifumi Yatani, Yoshinori Kamisaki

JOURNAL OF PHARMACOLOGICAL SCIENCES　103　164P-164P　2007

ISSN： 1347-8613
Dental Impressions/Gypsum Casts as a Potential Source of Microbial Cross-contamination

27　(2)　176-176　2006/12/20

ISSN： 0914-7713
Differentiation of osteoblast-like cells in oriented hydrogel

SASAKI J., MATSUMOTO T., EGUSA H., SOHMURA T., YATANI H.

Journal of the Japanese Society for Dental Materials and Devices　25　(5)　315-315　2006/09/25
Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)
ISSN： 0286-5858
印象体および石膏模型における微生物汚染の実態

綿本隆生, 江草宏, 福沢健次, 松尾孝章, 矢谷博文

口腔衛生学会雑誌 = JOURNAL OF DENTAL HEALTH　56　(4)　471-471　2006/08/30

ISSN： 0023-2831
3-dimensional culture of osteoblast-like cells in oriented hydrogel

SASAKI J., MATSUMOTO T., EGUSA H., YATANI H.

Journal of the Japanese Society for Dental Materials and Devices　25　(2)　183-183　2006/04/05
Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)
ISSN： 0286-5858
Involvement of calcineurin in osteoclastogenesis mediated by RANKL

T Iida, H Egusa, M Saeki, Y Itsuki, H Yatani, Y Kamisaki

JOURNAL OF PHARMACOLOGICAL SCIENCES　100　268P-268P　2006

ISSN： 1347-8613
Candida albicans 付着後の口腔上皮細胞の初期免疫応答

江草宏, 二川浩樹, 牧平清超, 矢谷博文, 浜田泰三

Bacterial Adherence & Biofilm　20　70-77　2006
2.骨髄由来幹細胞の分化へのDNAメチル化関与の可能性(一般口演,第3回日本再生歯科医学会学術大会および総会)

蘆田俊二, 江草宏, 小林宗正, 中村誠志, 矢谷博文, 西村一郎

日本再生歯科医学会誌　3　(1)　64-64　2005/12/30
Publisher: 日本再生歯科医学会
ISSN： 1348-9615
Significance of biopsychosocial interface in the management of temporomandibular disorders

Shoichi Ishigaki, Emiko Morishige, Hiroshi Egusa, Hirofumi Yatani

International Congress Series　1284　71-72　2005/09

DOI： 10.1016/j.ics.2005.06.098 　

ISSN： 0531-5131
Intercellular adhesion molecule 1-dependent activation of interleukin 8 expression in Candida albicans-infected human gingival epithelial cells

Hiroshi Egusa, Hiroki Nikawa, Seicho Makihira, Shoichi Ishigaki, Hirofumi Yatani, Taizo Hamada

International Congress Series　1284　213-214　2005/09

DOI： 10.1016/j.ics.2005.06.021 　

ISSN： 0531-5131
Candida adherence and biofilm formation on oral surfaces

Hiroki Nikawa, Seicho Mikihira, Hiroshi Egusa, Hitoshi Fukushima, Ryoko Kawabata, Taizo Hamada, Hirohumi Yatani

Japanese Journal of Medical Mycology　46　(4)　233-242　2005
Publisher: Japanese Society for Medical Mycology
DOI： 10.3314/jjmm.46.233 　

ISSN： 0916-4804
Significance of biopsychosocial interface in the management of TMD & orofacial pain. In Interface Oral Health Science

Elsevier International Congress Series　Vol. 1284, 71-72/,　2005
Methodology of microarray data analysis. In Immunohistochemistry and in situ Hybridization in Human Carcinomas

Elsevier Inc.　2005
骨髄由来幹細胞は遺伝子群の選択的抑制を介して分化する

江草宏, 西村一郎, 矢谷博文

Journal of oral biosciences　46　(5)　417-417　2004/09/01
Publisher: 歯科基礎医学会
ISSN： 1349-0079
バイオフィルムと義歯性口内炎

二川浩樹, 浜田泰三, 江草宏, 牧平清超, 福島整, 石田和寛

Bacterial Adherence & Biofilm　17　95-101　2003
Effects of Eluent of Tissue Conditioners on Human GingivalFibroblast in Vitro

ISHIDA K, NIKAWA H, NISHIMURA M, EGUSA H, NISHIMURA H, MURATA H, HAMADA T

46　(108)　180-180　2002/10/11
Analysis of β-defensins Expression in Human Oral Cells and their Fungicidal Effects

FURUKAWA M, NIKAWA H, MAKIHIRA S, EGUSA H, FUKUSHIMA H, KATAYAMA N, HAMADA T

46　(107)　170-170　2002/05/10
Responses of human gingival epithelial cells to Candida albicans infections.

H Egusa, H Nikawa, S Makihira, T Hamada

JOURNAL OF DENTAL RESEARCH　81　A406-A406　2002/03

ISSN： 0022-0345
Candida albicans感染に対する口腔上皮の細胞応答

牧平清超, 二川浩樹, 江草宏, 西付正宏

歯科基礎医学会雑誌　43　(5)　530-530　2001/08/20
Publisher: 歯科基礎医学会
ISSN： 0385-0137
Collagenolytic Activity of Candida albicans

YAMASHIRO H., NIKAWA H., NISHIMURA M., MAKIHARA S., EGUSA H., FUKUSHIMA F., ISHIDA K., NISHIMURA H., HAMADA T.

45　119-119　2001/06/01

ISSN： 0389-5386
Candidal Infection of Gingival Epithelial Cells : Ultrastructural and Biochemical Features

EGUSA H., NIKAWA H., MAKIHARA S., NISHIMURA M., YAMASHIRO H., JIN C., FUKUSHIMA H., NISHIMURA H., ISHIDA K., HAMADA T.

45　49-49　2001/06/01

ISSN： 0389-5386
Expression of Proteoglycans of Osteoblastic Cells Grown on Titanium

NISHIMURA H., NISHIMURA M., NIKAWA H., MAKIHARA S., YAMASHIRO H., JIN C., EGUSA H., ISHIDA K., FUKUSHIMA H., HAMADA T.

45　146-146　2001/06/01

ISSN： 0389-5386
デンチャープラークの病原性とそのコントロール～全身・口腔の健康のために～

二川浩樹, 浜田泰三, 金辰, 西村正宏, 牧平清超, 玉上真美, 山城啓文, 江草宏, 村田比呂司, 熊谷宏, 貞森紳丞

歯界展望増刊号　324　2001
Candida albicansの感染による口腔上皮細胞の細胞応答

江草宏, 二川浩樹, 牧平清超, 浜田泰三

Bacterial Adherence & Biofilm　15　39-45　2001
In Vitro Effects of Thyroid Hormone on Chondrocyte

MAKIHIRA S, NIKAWA H, ISHIDA K, NISHIMURA M, JIN S, EGUSA H, NISHIMURA H, FUKUSHIMA H, HAMADA T

44　(104)　205-205　2000/11/10
The Post-antifungal Effect (PAFE) of Polyene Antimycotics on Oral Candida albicans Isolates and Its Impact on the Cell Surface Hydrophobicity

EGUSA H, NIKAWA H, MAKIHIRA S, NISHIMURA M, YAMASHIRO H, JIN C, FUKUSHIMA H, HAMADA T

44　(104)　46-46　2000/11/10
Exposure to sub-therapeutic concentrations of polyene antifungals suppresses the adherence of Candida species to denture acrylic

EGUSA H, NIKAWA H, NISHIMURA M, MAKIHIRA S, YAMASHIRO H, CHEN J, HAMADA T

44　(103)　121-121　2000/06/09
Cytotoxic Effects of Eluates from Denture Base Acrylic on Gingival Cell Growth

MAKIHIRA S, NISHIMURA M, NIKAWA H, EGUSA H, SADAMORI S, MURATA H, YAMASHIRO H, JIN C, HAMADA T

44　(103)　120-120　2000/06/09
Deterioration of Resilient Denture Lining Materials Caused by Denture Cleansers : Surface Roughness and Color Stability

JIN C, NIKAWA H, MAKIHIRA S, YAMASHIRO H, EGUSA H, HAMADA T

44　(103)　52-52　2000/06/09
The adhesion of Candida albicans to intact and denatured collagen.

S Makihira, H Nikawa, M Tamagami, M Nishimura, H Yamashiro, C Jin, H Egusa, T Hamada

JOURNAL OF DENTAL RESEARCH　79　(5)　1241-1241　2000/05

ISSN： 0022-0345
The adhesion of Candida albicans to intact and denaturated collagen.

S Makihira, H Nikawa, M Tamagami, M Nishimura, H Yamashiro, C Jin, H Egusa, T Hamada

JOURNAL OF DENTAL RESEARCH　79　522-522　2000

ISSN： 0022-0345
Impacts of PAFE on the relative CSH of Candida albicans.

H Egusa, ANB Ellepola, H Nikawa, T Hamada, LP Samaranayake

JOURNAL OF DENTAL RESEARCH　79　151-151　2000

ISSN： 0022-0345
The Adherence of Candida albicans to the Denatured-type I collagen

TAMAGAMI M, NIKAWA H, MAKIHIRA S, NISHIMURA M, YAMASHIRO H, KIN S, EGUSA H, HAMADA T

43　(101)　58-58　1999/05/21
Candida albicansのⅠ型コラーゲンおよび変性Ⅰ型コラーゲンへの付着に関する研究

牧平清超, 二川浩樹, 玉上真美, 西村正宏, 山城啓文, 金辰, 江草宏, 浜田泰三

Bacterial Adherence研究　13　39-46　1999
An Evidence of Cariogenicity of Candida albicans Against Root Surfaces : An In Vitro Examination of Fungal Colonization of Type I Collagen

YAMASHIRO H, NIKAWA H, NISHIMURA M, MAKIHIRA S, TAMAGAMI M, KIN S, EGUSA H, HAMADA T

42　(100)　77-77　1998/11/26

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Books and Other Publications 12

よくわかる口腔インプラント学第4版

Hiroshi Egusa

医歯薬出版　2023
クラウンブリッジ補綴学第6版

江草宏

医歯薬出版　2021
Molecular Players in iPSC Technology, 1st Edition

Osathanon T, Egusa H

Ed: Birbrair A, Elsevier　2021
院内感染防止対策のスタンダード

デンタルダイアモンド社　2020/04
関節・軟骨の再生医療

堀江尚弘, 近藤威, 江草宏

シーエムシー出版　2019/12
クラウンブリッジテクニック第2版

江草宏

医歯薬出版　2018
口腔組織学・発生学第2版

江草宏

医歯薬出版　2015
冠橋義歯補綴学テキスト

江草宏

末永書店　2015
見分けて治そう！歯科金属・材料アレルギー

江草宏

クインテッセンス出版　2015
Textbook of preclinical training in fixed prothodontics

Egusa H, Nakano T, Mizumori T, Mutobe Y

Osaka University Press　2010
生命歯科医学のカッティング・エッジ（米田俊之編）

矢谷博文, 中村隆志, 瑞森崇弘, 石垣尚一, 山田真一, 江草宏

大阪大学出版会　2008
Handbook of immunohistochemistry and in situ hybridization of human carcinomas ~Molecular genetics: Liver and Pancreatic Carcinomas~ Vol. 3.

Zhou X, Egusa H, Cole SW, Nishimura I, Wong DTW

Elsevier Academic Press　2005

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Presentations 64

In vitro bone tissue fabrication by using iPS cells International-presentation Invited

EGUSA Hiroshi

The 96th IADR General Session　2018/07/26
小臼歯CAD/CAM冠の臨床評価から考える大臼歯CAD/CAM冠の勘どころ Invited

江草宏

第127回日本補綴歯科学会学術大会　2018/06/17
インプラント臨床における再生医療の現状と挑戦 Invited

江草宏

第37回日本口腔インプラント学会近畿・北陸支部学術大会　2017/12/03
iPS cell-based strategies in bone tissue engineering International-presentation Invited

江草宏

第65回 JADR学術大会国際シンポジウム “Advances in iPS cell research and its application to dental medicine”　2017/11/19
Emerging approaches for “regenerative prosthodontics” International-presentation Invited

EGUSA Hiroshi

2017/10/20
iPS細胞を用いた骨再生医療戦略 Invited

江草宏

日本バイオマテリアル学会東北ブロック講演会招待講演　2017/09/25
iPS cell-based strategies in bone tissue engineering International-presentation

EGUSA Hiroshi

The 2nd Annual Academic Conference of Society of Dental Research Administration, CSA　2017/08/25
JPRにおける企画総説論文 Invited

江草宏

第126回日本補綴歯科学会学術大会委員会セミナー「Journal of Prosthodontic Research―現状と今後の展開」　2017/06/30
再生医療の補綴歯科治療への展開 Invited

江草宏

第16回日本再生医療学会総会シンポジウム「歯科再生医療推進ネットワーク協議会の立ち上げと歯科再生医療の今後の展開」　2017/03/08
再生歯科医療を材料と生体から理解する Invited

江草宏

大阪口腔インプラント研究会シンポジウム「組織再生の最前線」　2016/11/13
iPS細胞が描く歯科医療の未来～これからの再生歯科医療への期待と課題～ Invited

江草宏

第69回東北地区歯科医学会特別講演　2016/11/05
iPS細胞を用いた骨組織再生技術の開発 Invited

江草宏

第59回春季日本歯周病学会学術大会シンポジウム「大規模歯周組織再生を目指した細胞治療開発の新展開」　2016/05/20
iPS細胞を用いた骨再生医療戦略 Invited

江草宏

第70回東京工業大学精密工学研究所シンポジウム「精密工学の新展開－医歯工連携から社会実装へ－」　2016/03/11
iPS cell-based strategies in bone tissue engineering International-presentation Invited

EGUSA Hiroshi

The 6th International Symposium for Interface Oral Health Science　2016/01/19
iPS cells: What they are and what they can contribute to the field of dentistry International-presentation Invited

EGUSA Hiroshi

Chulalongkorn-Tohoku Joint Symposium in Dental Science 2015　2015/12/09
再生歯科医療の現状と展望 Invited

江草宏

第67回近畿北陸地区歯科医学大会講演　2015/12/06
iPS cells and their expected role in the future of dentistry International-presentation Invited

EGUSA Hiroshi

The 3rd Sino-Japan Dental Science Symposium　2015/11/24
iPS細胞技術が描く歯科医療の未来 Invited

江草宏

平成27年度日本補綴歯科学会東北・北海道支部学術大会特別講演　2015/10/25
iPS cells: What they are and what they can contribute to the field of dentistry International-presentation Invited

EGUSA Hiroshi

Hong Kong University-Tohoku University 1st Bilateral Dental Symposium　2015/10/16
iPS細胞を用いた骨再生研究とインプラント臨床への展望 Invited

江草宏

第45回日本口腔インプラント学会学術大会シンポジウム「口腔インプラント治療や歯科治療は，生物学の進歩によってどう変わるか」　2015/09/22
iPS cells and their expected role in the future of prosthodontics International-presentation Invited

EGUSA Hiroshi

16th Biennial Meeting of International College of Prosthodontists　2015/09/18
iPS細胞が可能にする歯科医療とは Invited

江草宏

日本臨床歯周病学会第33回年次大会教育講演　2015/07/18
iPS cells: What they are and what they can contribute to the field of dentistry International-presentation Invited

EGUSA Hiroshi

Sichuan-Tohoku Dental Symposium 2015　2015/07/10
再生歯科医療の動向と展望 Invited

江草宏

大阪口腔インプラント研究会特別講演　2015/06/28
iPS細胞を用いた骨組織再生 Invited

江草宏

日本組織培養学会第88回大会シンポジウム「臨床のため細胞培養」　2015/05/27
歯科領域における再生医療の動向と展望 Invited

江草宏

第69回日本口腔科学会学術集会シンポジウム「再生医療の最前線」　2015/05/13
iPS cells: What they are and what they can contribute to prosthodontics International-presentation Invited

EGUSA Hiroshi

2015 Biennial Joint Congress of JPS-CPS-KAP　2015/04/11
Application of iPS Cells in Bone Biology International-presentation Invited

EGUSA Hiroshi

China-Japan-Korea Dental Science Symposium 2014　2014/11/08
iPS細胞が可能にする歯科医療とは Invited

江草宏

第73回日本矯正歯科学会学術大会特別講演　2014/10/21
iPS細胞の骨生物学への応用と歯科臨床への展望 Invited

江草宏

平成26年度日本歯科理工学会北海道・東北地方会夏季セミナー特別講演　2014/08/02
iPS細胞が可能にする歯科医療とは Invited

江草宏

第65回東北大学歯学会特別講演　2014/06/13
iPS細胞の骨形成能と歯科臨床への展望 Invited

江草宏

第68回日本口腔科学会学術集会シンポジウム「iPS細胞が拓く未来の医療」　2014/05/09
iPS細胞が可能にする歯科医療とは Invited

江草宏

第47回新潟歯学会総会特別講演　2014/04/19
Application of iPS Cells in Bone Biology International-presentation Invited

EGUSA Hiroshi

Kyudai Oral Bioscience 2014　2014/02/28
iPS Cells in Regenerative Dental Medicine International-presentation Invited

EGUSA Hiroshi

The 5th International Symposium for Interface Oral Health Science　2014/01/21
Stem Cells in Regenerative Dental Medicine International-presentation Invited

EGUSA Hiroshi

2013 Thailand International Conference on Oral Biology　2013/11/28
iPS細胞が可能にする歯科医療とは Invited

江草宏

平成25年度日本補綴歯科学会関西支部学術大会生涯学習公開セミナー「歯科におけるバイオサイエンスの基礎と応用」　2013/11/24
歯肉から作るiPS細胞が可能にする歯科医療とは Invited

江草宏

第25回日本小児口腔外科学会学術大会シンポジウム「小児口腔外科と再生医療」　2013/11/02
iPS細胞を用いたスキャフォールドフリー骨組織再生 Invited

江草宏

第55回歯科基礎医学会学術大会シンポジウム「Biodental Engineering ～生命科学研究と材料科学（理工学）の融合～」　2013/09/22
iPS細胞の骨生物学への応用と歯科臨床への展望 Invited

江草宏

第29回医学生物学電子顕微鏡技術学会特別講演　2013/06/09
iPS細胞の骨形成能と歯科臨床への展望 Invited

江草宏

第67回日本口腔科学会学術集会シンポジウム「歯周組織における再生医療」　2013/05/23
Application of gingiva-derived iPS cell technologies in dental research Invited

EGUSA Hiroshi

University of Washington Bioengineering Departmental Seminar　2013/03/21
Application of gingiva-derived iPS cell technologies in regenerative dental medicine International-presentation Invited

EGUSA Hiroshi

International Symposium on Human Resource Development towards Global Initiative　2013/02/16
Application of gingiva-derived iPS cell technologies in dental research International-presentation Invited

2012/12/15
Application of gingiva-derived iPS cells in bone biology International-presentation Invited

EGUSA Hiroshi

The 2nd International Anatomical Sciences and Cell Biology Conference　2012/12/07
歯肉由来iPS細胞の歯科医学への応用 Invited

江草宏

第54回歯科基礎医学会学術大会シンポジウム「口腔領域におけるiPS研究の現状と展望」　2012/09/14
iPS細胞の骨生物学への応用 Invited

江草宏

硬組織再生生物学会第21回学術大会特別講演　2012/08
Gingiva-derived iPS cells: A promising tool for dental applications International-presentation Invited

江草宏

大阪大学歯学部・大学院歯学研究科国際シンポジウム “Development and Regeneration of Oro-Facial Structures”　2012/07
Gingiva-derived iPS cells: A promising tool for dental applications International-presentation Invited

EGUSA Hiroshi

The 90th IADR General Session Symposium "Stem Cell Niches and Pluripotent Stem Cells for Craniofacial Regeneration"　2012/06
歯肉由来iPS細胞の口腔先端医学への応用 Invited

江草宏

口腔先端応用医科学研究会第4回学術会議招待口演　2012/01
歯肉をiPS細胞源とする歯科医療技術の創成 Invited

江草宏

日本歯科医学会第28回「歯科医学を中心とした総合的な研究を推進する集い」　2012/01
Gingiva-derived iPS cells: A promising tool for dental applications International-presentation Invited

EGUSA Hiroshi

The 4th Hiroshima Conference on Education and Science in Dentistry　2011/10
歯肉のあらたな可能性－医療応用に有望なiPS細胞源－ Invited

江草宏

日本口腔インプラント学会第41回学術大会教育講演　2011/09
口腔粘膜歯肉を用いたiPS細胞の樹立および骨生物学への応用 Invited

江草宏

日本骨代謝学会第29回学術集会若手シンポジウム「革新的バイオサイエンスが拓く，次世代Bone Biology」　2011/07
The Impact of Gingival Fibroblast-Derived iPS Cells in Dentistry International-presentation Invited

江草宏

The 3rd Thailand International Conference on Oral Biology 国際シンポジウム “Biology of Mineralized Tissues”　2011/05
歯肉をiPS細胞源とする補綴歯科治療の可能性 Invited

江草宏

日本補綴歯科学会第120回学術大会シンポジウム「バイオエンジニアリングが拓く補綴歯科イノベーション」　2011/05
歯肉のあらたな可能性－医療応用に有望なiPS細胞源－ Invited

江草宏

日本学術振興会産学協力研究委員会レドックス生命科学第170委員会レドックスライフイノベーションシンポジウム　2011/03
The Impact of Gingival Fibroblast-Derived iPS Cells in Dentistry International-presentation Invited

EGUSA Hiroshi

2011/03
歯肉を用いたiPS細胞の樹立および骨生物学への応用 Invited

江草宏

第9回口腔医科学フロンティア招待講演（選抜演者）　2011/03
Gingival fibroblasts as a promising source of induced pluripotent stem cells International-presentation Invited

EGUSA Hiroshi

2010/12
歯肉のあらたな可能性－医療応用に有望なiPS細胞源－ Invited

江草宏

第111回大阪大学歯学会例会平成22年度大阪大学弓倉学術奨励賞・受賞講演　2010/11
再生医学とインプラント治療－近未来のインプラント学におけるバイオテクノロジーのインパクト－ Invited

江草宏

日本補綴歯科学会第118回学術大会シンポジウム「インプラントと再生医療」　2009/06
オーファンリガンドライブラリーを用いた破骨細胞分化関連化合物のスクリーニング Invited

江草宏

第50回歯科基礎医学会学術大会シンポジウム「硬組織の明るい未来を目指して」　2008/09
Cyclic Strain Induces Cellular Alignment and Promotes Myogenic Differentiation of Bone Marrow Stromal Stem Cells International-presentation Invited

EGUSA Hiroshi

2008/02

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Industrial Property Rights 19

Method for producing osteoblast culture using uPS cells

Hiroshi Egusa, Hiroko Okawa, Naohiro Horie, Takeru Kondo

特許日本(第7692610号)

Property Type: Patent
振盪浮遊培養を用いた間葉系幹細胞の未分化性維持方法

新部邦透, 江草宏

特許日本(第7588806号)

Property Type: Patent
歯科用ユニットの給水配管内の水を殺菌するための装置及び方法

江草宏, 高橋信博, 山田将博, 鷲尾純平

特許日本(第7376106号)

Property Type: Patent
Method for maintaining undifferentiation potency of mesenchymal stem cell employing shaking suspension culture

Kunimichi Niibe, Hiroshi Egusa

特許米国(第11661584号)

Property Type: Patent

Holder: Tohoku Univresity
Method for producing osteoblast culture using uPS cells

Hiroshi Egusa, Hiroko Okawa, Naohiro Horie, Takeru Kondo

特許米国(第11459547号)

Property Type: Patent
口腔粘膜由来細胞を利用した誘導多能性幹細胞（iPS細胞）の効率的な製造方法

江草宏, 萱島浩輝, 矢谷博文

特許日本(第5514215号)

Property Type: Patent
Mesenchymal cell proliferation promoter and skeletal system biomaterial

EGUSA Hiroshi

特許米国(第8222216号)

Property Type: Patent
歯科用重合性覆髄材組成物

柿沼祐亮, 近藤威, 江草宏

Property Type: Patent
11. 表面処理チタン基材及びその製造方法、複合体、培養床、並びにハイドロキシアパタイトのナノ針状結晶の製造方法

山田将博, 江草宏

Property Type: Patent
骨補填材、及びその製造方法

江草宏, 大川博子, 森里美

Property Type: Patent
破骨細胞が関与する疾患の予防剤又は治療剤

江草宏, 佐伯万騎男, 上崎善規, 矢谷博文

特許日本(第6261048号)

Property Type: Patent
iPS細胞を用いた骨芽細胞塊の作製法

江草宏, 大川博子

Property Type: Patent
骨再生剤

江草宏

特許米国(第9974884号)

Property Type: Patent
骨再生剤

江草宏

特許日本(第6133433号)

Property Type: Patent
iPS細胞の腫瘍化を抑制することが可能な分化誘導方法

江草宏

特許米国(第9463204号)

Property Type: Patent
iPS細胞の腫瘍化を抑制することが可能な分化誘導方法

江草宏

特許日本(第5935224号)

Property Type: Patent
iPS細胞の腫瘍化を抑制することが可能な分化誘導方法

江草宏

特許中国(第ZL 2012 8 0063680号)

Property Type: Patent
口腔粘膜由来細胞を利用した誘導多能性幹細胞（iPS細胞）の効率的な製造方法

江草宏, 萱島浩輝, 矢谷博文

特許EU(第2474610号)

Property Type: Patent
口腔粘膜由来細胞を利用した誘導多能性幹細胞（iPS細胞）の効率的な製造方法

江草宏, 萱島浩輝, 矢谷博文

特許米国(第8748179号)

Property Type: Patent

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Research Projects 59

新たな細胞死「フェロトーシス」の制御に着目した新規顎骨増生技術の開発

渡辺隼, 江草宏

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2024/04/01 - 2027/03/31
細胞膜模倣バイオマテリアルの創製と迅速骨再生への応用

松本卓也, 江草宏, ハラエミリオ・サトシ, 岡田正弘

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 岡山大学

2022/04/01 - 2026/03/31
Development of the novel light-cured dentin regenerative cement

Takeru Kondo, Hiroaki Kakimuma, Hiroshi Egusa

Offer Organization: Japan Agency for Medical Research and Development (AMED)

System: Medical Device Research and Development Project: Challenge Type

2025/05 - 2026/03
新規ニッケル結合性T細胞を基軸としたニッケルアレルギー病態形成機構の解明

黒石智誠, 菅原俊二, 江草宏

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2022/04/01 - 2025/03/31
自然治癒力と骨質を高める細胞由来骨模倣材料による顎骨再生の新機軸

江草宏, 山田将博

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2022/04/01 - 2025/03/31
AI機械学習を基盤とした支台歯形成技能評価システムの自律化

奥山弥生, 江草宏, 熊澤逸夫, 勝田悠介, 山田将博

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2021/04/01 - 2024/03/31

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近年，デジタルデンティストリーの開発は目覚ましいものがあるが，補綴治療において，口腔内で長期に安定した治療効果を得るためには，適切な支台歯形態を与える必要がある．しかし，支台歯形態の良否の臨床的判断は，いまだ主観的な目視に依存している．研究代表者はこれまでに「支台歯形態評価ルーブリック」を開発してきたが，これは手動の過程を多く残しており，評価者に技能評価の多くを委ねているのが現状である．研究代表者らは，三次元形状デジタルデータの人工歯を対象に，教員によるルーブリックを用いた支台歯形態評価を教師とした機械学習を行い，種々の条件下で解析を重ね，高正解率の支台歯形成評価の最適解析方法を求めてきた．本年度，研究協力者であり，令和３年度生体医歯工学共同研究拠点プロジェクト研究課題の協同研究者である吉岡勇人准教授の指導の下，人工支台歯の三次元形状データから，咬合面の削除量および軸面のテーパー角度を算出する方法の解析が進められ，それぞれの自動評価システムを開発した．すなわち，支台歯形成済人工歯上に作成した断面の外形線を歯根側から順に走査し，偏角の変化率が小さい範囲を抽出するという幾何学的手法によって「軸面のテーパー部分」を抽出することに成功した．「軸面のテーパー」の評価アルゴリズムの確立は，本研究の根幹的課題の解決に相当するものと思われる．また，「咬合面の削除量」の評価を立体的な3Dデータ全体を評価対象とし，支台歯の形状特性を考慮し，小窩付近では立体的な抽出範囲を設定し，その範囲内での最短距離を算出する方法を確立した．その結果，より精度の高い評価結果を得ることができた．今後は，形成済人工歯の３次元データの次元数をそのまま解析対象とする最適解析方法を複数の評価項目に関して求め，次にその測定データをどう評価するか，歯科指導者による具体的な検討を開始する予定である．
Development of a vascularized organoids transplantation technology for next-generation regenrative medicine

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2021/04/01 - 2024/03/31
骨小腔－骨細管系ネットワークの制御を基盤とした即時負荷インプラントの開発

石橋実, 山田将博, 江草宏

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2020/04/01 - 2023/03/31

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研究実績の概要チタンインプラントのナノ表面改質が骨細胞の骨小腔―骨細管系ネットワークの構築に与える影響を評価した。ブタ腱由来酸抽出のI型コラーゲンゲル中に骨細胞株MLO-Y4を包埋し、チタン平滑面またはナノ表面ディスク上で培養した。培養7日後に、共焦点レーザー顕微鏡観察およびRT-PCR解析を行った。その結果、チタン平滑面上では、コラーゲンゲル中で細胞突起を伸ばすものの、ギャップジャンクションの発現を伴う細胞間相互連結は乏しかった。一方、ナノ表面上のコラーゲンゲル中の骨細胞は神経細胞様の細胞突起の伸展とともにギャップジャンクションの高発現を伴う細胞間相互連結が発達した。チタン表面上に付着した骨細胞は、平滑面上では多角形に伸展しただけであったが、ナノ表面上では著しい接着斑の形成と細胞突起の伸展を伴う成熟した骨細胞が観察された。以上の結果により、チタンナノ表面は骨細胞ネットワーク構築を促進することが示唆された。
New innovation of jaw bone reconstruction using octacalciumphosphate/collagen composite and iPS cells

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2020/04/01 - 2023/03/31
バイオ3Dプリンティング技術を応用した歯周組織再生型インプラントの開発

山田将博, 江草宏, 中山功一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2020/04/01 - 2023/03/31

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近年，標的組織の三次元的構造と機能をもつ組織体を創出するバイオ3Dプリンティング技術が再生医療分野で注目を浴びている．研究代表者はこれまでに，歯根セメント質の理工学的性質を模倣して，歯根疑似環境を提供することで，歯根膜細胞の分化をセメント芽細胞へと導き，歯周組織の誘導を可能にするチタンナノ表面改質法の開発に取り組んできた．以上を背景に，本研究は，バイオ3Dプリンティング技術とチタンナノ表面改質技術とを組み合わせて，「歯周組織再生型インプラント治療」の確立に向けた「生体外でインプラント周囲に歯周組織体を三次元的に誘導する組織工学基盤技術」の構築を目的とする． 2年目となる2021年度では、歯根膜細胞構造体をラット上顎骨モデルに埋植し、歯根膜細胞構造体の口腔内移植モデルの確立を目指すとともに、本研究計画のベースラインデータとなる歯根膜細胞構造体の骨組織中での生体反応を組織学的に解析した．また、チタンナノ表面が骨免疫に与える影響を評価した．野生型ラット上顎第一大臼歯を抜去し、骨欠損を作製後にチューブ状の歯根膜細胞構造体を埋植した．細胞構造体の脱落を防ぐために、チタン膜にて骨欠損部を被覆固定する術式を開発した．細胞構造体埋植4週後に組織学的に解析した結果、歯根膜細胞構造体単独では、骨欠損部内に歯周組織は再生されず、新生骨形成を促進することが明らかとなった。現在、歯根膜細胞構造体をチタンインプラントに巻き付けた複合体の作製を行っている。また、生体材料の骨免疫反応に関与するマクロファージの分極にチタンナノ表面が与える影響を細胞培養試験的に評価した結果、生体模倣チタンナノ表面はマクロファージをM1型に分極するとともに破骨細胞による炎症性骨吸収を抑制する因子の産生へとマクロファージの機能を制御することが明らかとなった。
疾患由来iPS細胞レジストリとPLAP-1を基軸とした侵襲性歯周炎の分子病態解明

山田聡, 森崎隆幸, 江草宏, 鈴木茂樹, 根本英二, 村上伸也, 梶川哲宏

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2020/04/01 - 2023/03/31

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本研究では、未だその発症・進行メカニズムの詳細が不明な侵襲性歯周炎（AgP）において、患者由来iPS細胞レジストリ（AgP-iPS細胞レジストリ）を構築し、同細胞レジストリを用いて網羅的な全ゲノム解析と細胞分化機能解析を同時に行うことで、侵襲性歯周炎の原因となる複数の新たなる遺伝子変異群を決定することを目的としている。さらには、申請者らのこれまでの研究結果から、侵襲性歯周炎原因遺伝子の一つであることが示唆されるPLAP-1/Asporinについて、AgPレジストリにおけるPLAP-1遺伝子多型解析を行うことで、PLAP-1が、侵襲性歯周炎の原因遺伝子であることを証明することを目的としている。2021度は、侵襲性歯周炎患者由来iPS細胞レジストリ構築のため、侵襲性歯周炎患者の歯周基本治療および歯周外科処置時に通常は廃棄される不良肉芽組織を回収し、回収した組織からiPS細胞作製用の培養線維芽細胞を樹立するための学内倫理研究申請を行った。さらには、健常者由来iPS細胞を用いてiPS細胞の培養系ならびに間葉系間質細胞と骨芽細胞分化誘導系の確立を行っている。さらに、侵襲性歯周炎と診断された患者の末梢血からゲノムDNAを抽出し、同DNAを大阪大学侵襲性歯周炎ゲノムバンクへ送ることで、レジストリを構築した。また、PLAP-1遺伝子多型が見られるアスパラギン酸リピート数の差異がPLAP-1タンパクの機能におよぼす影響を解析することを目的として、リピート数の異なる哺乳類細胞由来組み換えPLAP-1タンパクを発現・作製するためのベクターシステムを構築した。
iPS細胞を原材料とした骨形成誘導補填材の開発

江草宏

Offer Organization: 国立研究開発法人日本医療研究開発機構

2020/11 - 2022/04
Challenge of controling tooth root length by HDAC3 inhibition of dental mesenchym

Niibe Kunimichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2019/04/01 - 2022/03/31

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The objective of this study is to control tooth root by inhibiting HDAC3 of dental mesenchyme. Our MSC-spheroids which showed neural crest stem cell like phenotype highly expressed HDAC9, HDAC11 and down regulated ESCO2, AURKA, and AURKB by analyzing PCR array. Our experiments suggested that the epigenetic change of MSC-spheroids was related to the gene expression of cell proliferation and differentiation. In addition, we performed tooth organ germ method by using MSC-spheroid and fetal dental epithelium. We successfully generate tooth organ using our MSC-spheroids. The next step, we will use HDAC inhibitor to organ germ to make clear whether HDAC inhibitor can control tooth root length.
Development of Bone Organoid Models Towards the Precision Prosthodontics

Egusa Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2019/04/01 - 2022/03/31

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Recently, organoid-on-a-chip technologies, which enable us to reproduce biological function of cells and tissues at the organ level in vitro, commands considerable attention. The aim of this study was to establish culture methods for the bone/cartilage/tooth organoids-on-a-chip using induced pluripotent stem (iPS) cell technology towards creating precision prosthodontics. We established induction method of iPS cells to bone organoid using micro-space culture wells. We also found a method to induce the bone/cartilage hybrid by applying a shaking culture. Furthermore, we established a step-wise culture method to induce iPS cells to enamelblasts. These results would contribute to the production of bone/cartilage/tooth organoids-on-a-chip in the future, and may lead to the basic technology of precision prosthetics that reproduces patient hard tissues in vitro.
Analysis of bone metabolism for predictable extra-vertical bone augmentation

Kayashima Hiroki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Osaka University

2018/04/01 - 2021/03/31

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This study revealed thatextra-vertical bone formation was possible by using CAD/CAM titanium molds with β-TCP, BBM, and carbonate apatite (CO3Ap). As a result of birefringence microscopy, no regular collagen orientation was observed in the BBM and CO3Ap groups. In the β-TCP group, no regular collagen orientation was observed in the interior and the apex of the augumented bone, but the other surface layers of the augumented bone hAs a result of SEM image observation, BBM group and CO3Ap group showed a lot of granules in horizontal section, while β-TCP group showed almost no granules.ad collagen orientation at an angle similar to the dome tangent and continuous with the original bone.
Development of a machine learning-based automatic evaluation system for clinical skills in tooth preparation

Okuyama Yayoi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2018/04/01 - 2021/03/31

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A systematic evaluation system for tooth preparation skills has not been established. We tried a machine learning-based automatic evaluation system for clinical skills in tooth preparation. The students prepared the upper second premolars for CAD/CAM composite crown in manikin head. At the end of the class, the instructors evaluated the tooth preparation skills of these students using a scoring rubric. The results were called “instructors’ score”. These prepared teeth were scanned and labeled into STL-format data tooth model. We focused on ‘taper’ and used 1D-CNN. Our results show that the accuracy was 61.38% between “instructors’ score” and “predicted class”. For the first time, we proposed machine learning-based automatic evaluation system for clinical skills in tooth preparation.
Elucidation of organ determination mechanism in epithelial / endothelial invaginated tissue

Fukumoto Satoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

2017/04/01 - 2021/03/31

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The purpose of this study is to comprehensively analyze gene expression at the developmental stage of teeth and to collect the knowledge necessary for organ regeneration. Using the CAGE and scRNAseq method, we succeeded in identifying the differentiated markers of all cell groups that differentiate from invaginated epithelial cells. In the analysis of individual molecules, Sox21 was found to be an important molecule that determines whether the invaginated epithelium becomes a tooth or a hair. Furthermore, Gpr115, which is strongly expressed in ameloblasts, functions as a pH sensor in the process of tooth calcification and plays an important role in promoting calcification. These findings were considered to be new findings in tooth development and basic findings to clarify the fate determination of various organs.
Self-organization of iPS cells using environmental factors in the tooth development

Egusa Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Tohoku University

2018/06/29 - 2020/03/31

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This study was aimed to establish a tooth engineering protocol by self-organization of iPS cells. Transcriptional regulation system was applied to mouse iPS cells in 3D culture to control expression of a tooth development-related gene. As a result, iPS cell aggregates formed cystic structures, which express oral ectoderm marker molecules. A step-wise induction method was also established to guide mouse iPS cells to differentiate into dental epithelial cells. When mouse iPS cells were cultured in hydrogels with different stiffness, effects of the stiffness on expression of tooth development-related genes were not significant. These results would contribute to promote iPS cell-based tooth engineering/regeneration in future.
Prospective clinical study of monolithic zirconia crowns-Following morphology and function for long-term prognosis-

Shoko Miura

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

2017/04/01 - 2020/03/31

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The purpose of this study was to examine the clinical performance of monolithic zirconia crowns in terms of short-term failure or complications. A prospective cohort study based on record evaluation and clinical examination of patients treated with tooth-supported monolithic zirconia crowns was performed in Japan. The crowns were prepared during the follow-up period from April 2014 to July 2018. The 3.5-year cumulative success and survival rates were set as primary endpoints. During the study period, 40 monolithic zirconia crowns were placed. Four crowns experienced clinical complications. The estimated Kaplan-Meier 3.5-year success and survival rates were 90.5% and 92.8%, respectively. The results of this study suggest that the molar application of monolithic zirconia crowns requires detailed attention to interocclusal clearance and the restoration of the antagonist tooth.
Development of genetic risk prediction method for tailor-made treatment for chronic orofacial pain

YATANI HIROFUMI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Osaka University

2017/04/01 - 2020/03/31

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In this study, we clarify the rate of non-repositioning articular disc dislocation by analyzing the MRI images of patients who presented with the symptoms of temporomandibular disorders, and the degree of deformity of the joint caused by the degree of disc dislocation. It was found to be associated with arthropathy. In addition, MASSARRAY analysis was performed using DNA samples collected from the buccal mucosa of the group with degenerative condylar changes of the mandibular condyle (OA group) and the group with normal temporomandibular joint (non-OA group). As a result, there was a significant difference in the SNP allele distribution of the FTO and TP63 genes between the OA and non-OA groups, suggesting that the SNPs of these genes may be involved in the development of osteoarthritis of the temporomandibular joint. Furthermore, there was a certain number of healthy subjects with signs of obstructive apnea syndrome, which is a risk factor for temporomandibular disorders.
Induction of periodontal ligaments on titanium implants mimicking nanotopography of cementum

Masahiro Yamada

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2017/04/01 - 2020/03/31

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The purpose of this study is to create the titanium nano-surface mimicking a cementitious surface and to induce cementum on the titanium implant through regulation of periodontal ligament cells. Cementum mimetic titanium nano-surface might reconstruct the periodontal tissue with cementum formation on the titanium implant in the presence of mesenchymal stem cells and periodontal ligament matrix.
Development of bone regeneration method for self-assembly of size-controlled iPS cell spheroid

Yatani Hirofumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Osaka University

2017/06/30 - 2019/03/31

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This study revealed that mouse iPS cells could control the size of the spheroids using micro well plates with different sizes and shapes by the number of seeded cells. As the result of observing the internal structure of the spheroids in each size, the spheroids of the small size was conposed of a single structure. On the other hand, as the spheroids size increased, these spheroids was conposed of a two-layered structure, and the central part may be caused in necrosis. The induction of osteogenic differentiation of iPS cell spheroids revealed that the reactivity of the compounds and self-assembly differed depending on the spheroids size.
Exploring nano-surface topography of dental implants to regulate osteoimmunology

Yamada Masahiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Tohoku University

2017/06/30 - 2019/03/31

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The cell culture experiment using mouse macrophage (MΦ) cell line showed that the polarization of MΦ is controlled on the titanium nano-roughened surface in contrast with the machined titanium surface. In addition, histological evaluations of peri-implant bone resorption around titanium implants were performed in a peri-implantitis model on rat maxilla. Severe gingival inflammation and bone resorption were observed in the peri-implant tissue around the machined titanium implants. In contrast, despite inflammatory reactions in peri-implant gingival tissue, gingival degradation and bone resorption were hardly observed in the peri-implant tissue around titanium implants with the nano-roughened surface. Those results suggested that nano-roughened titanium surfaces may inhibit the gingival degradation and bone resorption due to bacterial infectious inflammation by promoting the polarization of MΦ.
A study of bioactive materials used for bone filling

Suzuki Osamu

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Tohoku University

2017/06/30 - 2019/03/31

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The present study was designed to investigate about how chemical environment around calcium phosphate crystals affects proteins accumulation onto the crystals. Co-precipitation of bovine serum albumin as a model protein for active molecules, such as proteins in immunomodulation, was controlled by the solution calcium and phosphate ions environment under physiological conditions. The results suggest that the design concept to accumulate the proteins on the materials. The concept of the materials accumulating active molecules obtained in the present study could be used for the tissue regeneration.
Molecular biological search for non-infectiously induced peri-implant bone resorption

Ishibashi MInoru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2016/04/01 - 2019/03/31

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The present study purposed to establish an animal experimental model of non-infectious peri-implant bone resorption and to explore its molecular biological mechanism. First, Staphylococcus aureus strains were cultured on various titanium disks to identify the implant surface property with low infection sensitivity. It was found that smooth surface or nano-rough surface had lower infection sensitivity than micro-rough surface. Next, the influences of oxidative stress associated with noninfectious bone resorption on bone healing were examined. As a result, oxidative stress induced apoptosis and senescence in mesenchymal stem cells and hence, significantly inhibited bone healing. In contrast, conditioning of mesenchymal stem cells with an antioxidant suppressed induction of apoptosis and promoted bone regeneration after cell local transplantation into a bone defect.
Development of iPS cell-based tooth regeneration techniques by a transposon-based gene delivery system

Egusa Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2016/04/01 - 2019/03/31

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The aim of this study was to develop induced pluripotent stem (iPS) cell-based tooth regeneration techniques by a transposon-based tetracycline (tet)-controlled gene regulation system. We focused on BMP-4, an important gene for tooth germ development, and successfully established a tet-controlled BMP-4 gene regulation system for iPS cells in which transcriptional activation of BMP-4 was associated with enhanced mesodermal and non-neuroectodermal lineage commitment. These findings represent an important step toward genetically engineered self-organization of iPS cells, particularly for generation of mesodermal cartilage and non-neuroectodermal organs, such as teeth.
Investigation of human iPS cell derived 3D bone like tissue using hypoxic culture

Okawa Hiroko, EGUSA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Research Activity Start-up

Institution: Tohoku University

2016/08/26 - 2018/03/31

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Induced pluripotent stem cells (iPS cells) are expected as a stem cell source to be used for regenerative medicine of severe alveolar bone loss. In order to develop iPS cells into clinical application, it is necessary to investigate method of osteogenic differentiation of human iPS cells. In this study, we found that hypoxic culture promotes osteogenic differentiation of iPS cells. It is expected that the results of this research will develop into the establishment of new bone augmentation using iPS cells.
Establisment of regenerative techique for jaw bone using MSCs spheroids cultureing with floating condition.

Niibe Kunimichi, EGUSA Hiroshi, MATSUZAKI Yumi, Zhang Maolin, OHORI Yumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (B)

Institution: Tohoku University

2016/04/01 - 2018/03/31

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We successfully established the newly culture method for mesenchymal stem cells (MSCs) to keep their stem-ness under shaking condition. MSCs are getting spheroids with shaking culture method. MSC spheroids can supply undifferentiated MSCs for a long period. This technology is patent pending. Moreover, We try to generate bone tissues with MSC spheroids to apply jaw bone regeneration. From our results, MSC spheroid can re-attach on a culture dish. Then, migrated cells from MSC spheroids are positive for alkaline phosphatase (ALP) after osteogenic induction. However, there are few positive cells in spheroids itself after ALP staining. It suggested that MSC spheroid kept their stem-ness robustly. From these findings, we are trying to establish the newly osteogenic induction method using MSC spheroids.
Development of a method for the endochondral alveolar bone tissue engineering using iPS cells

Egusa Hiroshi, SAITO Masahiro, FUKUMOTO Satoshi, OKAWA Hiroko, KOSAKA Yukihiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2016/04/01 - 2018/03/31

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A strategy of induced pluripotent stem (iPS) cell-based alveolar bone regeneration is expected to improve treatment outcome in prosthetic treatments. We focused on possible advantage in endochondral bone regeneration in the large bone defect. The aim of this study was to develop a method to fabricate bone/cartilage-like cell constructs using iPS cells. We found that combination use of chondrogenic and osteogenic induction factors could guide mouse iPS cell embryoid bodies into hybrid bone/cartilage-like cell constructs on the manipulation of a ratio in degrees of chondrocyte/osteoblast differentiation.
The surface morphology of titanium plate could change macrophage activity.

OKUYAMA YAYOI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2015/04/01 - 2018/03/31

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Different surface textured titanium plate such as mechanical polishing surface (MS) and acid treatment surface (AS) were prepared. RAW 264.7 cells known as osteoclast precursor cells were cultured on each surfaces, and their gene expression was examined. As a result, the expression of NOS2, a marker of inflammatory macrophages (M1 macrophages), increased in RAW 264.7 cells cultured on the MS surface. On the other hand, RAW 264.7 cells cultured on the AS surface showed similar to macrophages cultured on polystyrene (negative control). RAW 264.7 cells were cultured on the MS surface and the AS surface,and after 72 hours the culture supernatant was collected. By continuing detailed studies in the future, we plan to clarify how titanium surface properties affect macrophage activity.
Fracture factors of full zirconia ceramic restoration with mechanical analysis in prospective clinical study.

Kasahara Shin

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2015/04/01 - 2018/03/31

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This study evaluated the influence of different finish line designs and abutment materials of bilayer and monolithic zirconia crowns using three-dimensional finite element analysis. Three-dimensional models of two types of premolar crowns, an yttria-stabilized zirconia framework with veneering ceramic and a monolithic zirconia ceramic, were used in the analysis. Cylindrical models with the finish line design of the crown abutments were prepared with three types of margin curvature radius; R0: shoulder margin, R0.5: rounded shoulder margin and R1.0: deep chamfer margin. Two abutment materials, dentin and brass, were analyzed. In the FEA model, 1 N was loaded perpendicular to the occlusal surface at the center of the crown. For all crowns, stress was localized to the occlusal loading area as well as to axial walls of the proximal region. The lowest maximum principal stress values were observed when the dentin abutment with R0.5 was used under a monolithic zirconia crown.
Epiprofin regulation in cell proliferation and differentiation in tooth development

Nakamura Takashi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2015/04/01 - 2018/03/31

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We previously identified Epiprofin(Epfn) as a novel member of Sp transcription factor family that is expressed in certain ectodermal organs, such as teeth, hair follicles, nails and skin. During tooth development, Epfn regulates cell proliferation as well as cell migration at the early stages and cell differentiation in the later mature stage by controlling the phosphorylation of Epfn. We also identified a small molecule that strongly induced the expression of Epfn in dental epithelial cells. We are applying the Epfn -inducible small molecule in enamel biosynthesis of regenerative dentistry.
Development of jaw bone resorption suppression technique targeting glutamate transport protein PICK1

Kamano Yuya, EGUSA Hiroshi, SAEKI Makio

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Research Activity Start-up

Institution: Tohoku University

2015/08/28 - 2017/03/31

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For severe bone resorption, the development of new therapeutic methods based on the regulate bone metabolism factors is an important issue. In this study, we focused on glutamate, which is a neurotransmitter that has the role of regulating bone metabolism, so we studied PICK1 which bind to AMPA glutamate receptor. Over-expression of PICK1 significantly promoted osteoclast differentiation of osteoclast precursor cells and PDZ domain binding inhibitor of PICK1 significantly inhibited osteoclast differentiation. On the other hand, over-expression of PICK1 of osteoblast precursor cells did not affect osteoblast differentiation. From the above, it is suggested that PICK1 does not affect osteoblast differentiation and regulate osteoclast differentiation.
Vertical Bone Augmentation Using iPS Cell Constructs with CAD/CAM Dome

Yatani Hirofumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Osaka University

2015/04/01 - 2017/03/31

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It was revealed that the PKC inhibitor (isoH-7) promotes osteogenic differentiation of iPS cells. We also examined the mold design of dome type titanium membrane using CAD / CAM, and fabricated the mold for vertical bone augmentation. Furthermore, iPS cell aggregates differentiated into osteoblasts by adding PKC inhibitor were transplanted into the back of mouse. As the result, tumor formation was confirmed, but the tumor size became smaller as compared with that induced without PKC inhibitor. It was suggested that by improving the protocol it could be a new bone formation methods using autologous iPS cells combined with the vertical bone augmentation mold.
Development of novel therapeutics for the treatment of marfan syndrome using iPS technology

Saito Masahiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2014/04/01 - 2017/03/31

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Marfan syndrome (MFS) that is a severe, systemic disorder of connective tissue with predominant involvement of the ocular, cardiovascular and musculoskeltal system. Cardiovascular manifestations in the form of dissecting thoracic aortic aneurysms, mitral valve prolapse and myocardial dysfunction are the major cause of morbidity and mortality in MFS. The molecular pathogenesis of MFS proceeded principally through the activation of TGF-beta signaling and metalloproteinase expression have shown to cause as a common mechanisms that leads to aortic degeneration. Here we showed the novel mechanisms of ADAMTSL6beta on the accelerating ADAMTS4 activity to progress aortic aneurysm and dissection in MFS through versican degradation. Thus, our results suggest that recruitment of ADAMTS4 to fibrillin-1 microfibril by ADAMTSL6beta promotes versican degradation and that this axis may provide a new insight for pathogenesis of aortic aneurysm and dissection in MFS.
Regulation and determination of organ derived from invaginated epithelium

FUKUMOTO SATOSHI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2014/04/01 - 2017/03/31

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Tooth and hair are formed by invagination of epithelium. Invaginated epithelium interacts with neural crest derived mesenchymal tissues, and then formed tooth and hair. However, mechanism of organ determination has never clearly understood. Sox21 is one of the transcription factor, and expressed in dental epithelium. Sox21 knockout mice showed hair formation in incisor indicating that Sox21 may be involved in the determination of organs. Decrease Sox21 inhibited ameloblast marker gene expression, and induced hair marker genes including keratins. ChipSeq analysis showed that Sox21 directly binds to Anapc10 gene promoter region and regulated their expression in dental epithelium. In Sox21 knockout mice, Anapc10 expression was decreased in tooth germ. Further, Sox21 over expression induced Anapc10 expression. These results suggested that Sox21 regulates Anapc10 expression and organ determination.
Development of diagnostic technology for establishment of tailor-made prosthodontic treatments

Yatani Hirofumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Osaka University

2013/04/01 - 2017/03/31

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In physiological approach, human and animal studies suggest that sympathetic tone and stress can play roles in the intra-individual fluctuation of sleep bruxism activity while age/development and behavioral characteristics can be a contributing factor for the intra-individual difference of masticatory muscle activity during sleep. In cell biology approach, we aimed to find patient-dependent biological factors responsible for cluster failure of osseointegration in dental implants using patient-derived iPS cells. During the course of experiments, an efficient osteogenic induction protocol for iPS cells was established. In regard to a material science approach, the material-human interface with the individual difference of tooth was evaluated. In consideration of individual differences of teeth as a random effect, the adhesion position, the void existence at interface and the adhesion area size were detected as a significant factor for bond strength by the liner mixed effect model.
Fabrication of bone graft materials using iPS cells

Egusa Hiroshi, YATANI Hirofumi, FUKUMOTO Satoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2014/04/01 - 2016/03/31

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iPS cells can be infinitely expanded and guided to differentiate into osteogenic cells. In this research project, we attempted to fabricate bone graft materials by inactivation of osteogenically-induced iPS cells. Embryoid bodies of mouse gingiva-derived iPS cells were used to fabricate osteogenic iPS cell constructs. We found that the size and calcium production of mouse gingiva-derived iPS cell constructs can be controlled under a floating culture condition to provide suitable characteristics as a bone grafting material that enables bone regeneration in an animal model.
Establishment of iPS cell-based bone tissue engineering using small molecule compounds

Egusa Hiroshi, YATANI Hirofumi, MATSUMOTO Takuya, SAEKI Makio

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2013/04/01 - 2016/03/31

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Bone regeneration and augmentation in dental treatments cannot be easily accomplished because conventional bone grafting materials are not osteoinductive. Induced pluripotent stem cells (iPS cells) are expected to be useful for alveolar bone augmentation/regeneration; however, their clinical application will require complete differentiation toward an osteogenic cell fate to avoid tumor formation. In this research project, we found several small molecule compounds, which enhance osteogenic differentiation of iPS cells, and that application of statins to iPS cell constructs enables efficient bone augmentation and suppresses tumor formation in an animal model. These findings represent an important step toward the therapeutic application of iPS cells to bone tissue engineering in dentistry.
Development of technologies for next-generation regenerative therapies

Tsuji Takashi, KUBOKI Takuo, OSHIMA Masamitsu, EGUSA Hiroshi, TSUBOTA Kazuo, KAGIHARA Yasuhiro, FUJITA Satoshi, KISHIDA Akio, SATO Akio, TAKEDA Akira, TOYOSHIMA Ko-ei

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

2013/04/01 - 2016/03/31

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In this project, we aimed at the development of fundamental technology for the next generation of organ regenerative medicine. 1) We successfully demonstrated to regenerate of secretory glands including salivary and lacrimal glands by the transplantation of their bioengineered germs, which were reconstituted by using "Organ Germ Methods". 2) To identify the cell seeds for organ regeneration, we demonstrated the possibility for the development of the functional bioengineered 3D integumentary organ system and the realization of organ replacement therapy by using iPS cells. 3) We further achieved the fundamental technology developments of new functional gel materials using the functional sugar chain and self-assembling peptides, for the application to clinical application for organ regenerative medicine. These results indicated that we successfully developed the fundamental technology for clinical application of the wide variety of organ regenerative therapies.
Development of the new method to control the resorption of alveolar bone, targeting receptor acting on bone - nervous system

YATANI HIROFUMI, EGUSA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Osaka University

2013/04/01 - 2015/03/31

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Severe resorption of alveolar bone after tooth loss/extraction, makes it difficult to restore the missing teeth with dental implants or prosthodontic approaches. Bone resorption is the unique function of the osteoclast, and anti-resorption therapy to date has targeted this cell. Objectives of this study is to investigate the involvement of MLA in osteoclastogenesis. MLA significantly inhibited the formation of TRAP-positive multinucleated osteoclasts in a concentration-dependent manner. These results suggest, that MLA suppresses osteoclastogenesis and that application of MLA enables to promote new bone formation. These findings represent MLA may be useful for therapeutic strategies against alveolar bone resorption in prosthetic treatments.
Development of the iPS cell-based diagnostic technology for dental treatments

EGUSA Hiroshi, YATANI Hirofumi, NAKANO Tamaki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Osaka University

2012/04/01 - 2014/03/31

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Induced pluripotent stem (iPS) cells, which can be generated individual patients, have potential to represent the patient-specific cell responses in vitro. In this study, we attempted to generate integration-free iPS cells from patients' gingiva using episomal plasmid vectors, and successfully generated iPS cell clones. We next evaluated optimal osteogenic induction of iPS cells using mouse gingiva-derived iPS cells, demonstrating that iPS cells can be guided to differentiate into mature osteoblasts with hydroxyapatite formation. These results suggest that the firm method to guide integration-free iPS cells to differentiate into osteoblasts may lead to a new technology for detecting individual differences in the clinical outcome of bone regeneration.
multi-analysis of bone-implant interface using X-ray analysis and electron tomography

TAKESHIGE Fumio, MIURA Jiro, EGUSA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Osaka University

2011 - 2012

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Bone and Titanium are mineralized tissue and hard material. It is difficult to prepare thin specimens or surface fabrication of these tissues for transmission and scanning electron microscope observation without demineralizing them. We present a novel method of creating a cutting surface including the bone and titanium interface using the focus Ion beam (FIB) method and Scaninig Ion Microscope (SIM). This method is appropriate for nano-fabrication of thin specimens for brittle and hard materials such as bone and titaniumu. The major advantage of this method is its ability to visualize ultra-structurure in interface of highly calcified specimens without demineralization.
Development of virus-free generation and xeno-free culture of human gingiva-derived iPS cells

YATANI Hirofumi, EGUSA Hiroshi, YU Guannan

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Osaka University

2011 - 2012

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iPS cells are expected to be a powerful tool for regenerative treatments; however, problems that need to be addressed prior to clinical application include viral-vector-derived insertional mutagenesis and the use of animal-derived feeder cells for culture. We generated human gingival fibroblast (hGF)-derived iPS cells with episomal plasmid vectors. The resulting iPS cell clones could be maintained on hGF feeder cells. The virus-free and autologous culture of hGF derived-iPS cells thus represents an important step toward the future therapeutic usage of iPS cells.
Technology creation of iPS cell-based alveolar bone regenerationusing the gingiva as a cell source

EGUSA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (A)

Institution: Osaka University

2010 - 2012

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iPS cells can be generated from somatic cells by forced expression of defined exogenous factors. We found that the gingiva, which is often resected during general dental treatments and treated as biomedical waste, is a promising source of iPS cells. These iPS cells were capable of osteogenic differentiation, which could be greatly enhanced by some small molecules. The generation of iPS cells from the gingiva offers a promising method for the facile production of iPS cells by dentists, which represent an important step toward the development of novel alveolar bone regeneration technology.
Development of bone resorption inhibitors/drugs targeting signaling molecule PICK1

AKASHI Yoshihiro, EGUSA Hiroshi, KAMANO Yuya

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Osaka University

2010 - 2012

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Novel molecular mechanisms and molecular targets for osteoclastogenesis could form the basis for effective therapeutic strategies against alveolar bone resorption. We previously identified PICK1 (protein interacting with C kinase) as a novel calcineurin B-binding protein that modulates NFAT activity in neuron-like PC12 cells. In this study, we found that PICK1 binds to calcineurin B in osteoclast progenitor cells and promotes osteoclast differentiation via the activation of calcineurin-NFAT signaling. The novel molecular target PICK1 may be useful for therapeutic strategies against alveolar bone resorption in prosthetic treatments.
Development of cell-based screening system for identifying osteogenesis-targeting compounds

YATANI Hirofumi, EGUSA Hiroshi, SAEKI Makio, FUKUYASU Sho

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Osaka University

2010 - 2012

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Small-molecule compounds that potently affect osteoclastogenesis or osteogenesis could form the basis for effective therapeutic strategies for alveolar bone regenerative medicine. In this study, we established osteoclast progenitor cell (NFAT/luciferase reporter cell)- or osteoblast (type I collagen/GFP reporter cell)-based high-throughput screening systems. These screening systems could identify candidates for osteoclastogenesis- or osteogenesis-targeting compounds in reliable manner, thus making it a promising tool for finding novel synthetic regulators of bone formation.
Analysis of bone regeneration using small-molecule harmine and Id2-knockout mice

EGUSA Hiroshi, YATANI Hirofumi, SAEKI Makio, YOKOTA Yoshifumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Osaka University

2010 - 2011

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This research project was performed aiming at investigating new molecular mechanisms using Id2-knockout mouse-derived osteoclasts/ osteoblasts and small-molecules. We found that small-molecule harmine is a potent activator of NFATc1 due to its interference with the functions of the protein kinase DYRK1A in osteoclast progenitors, and via its induction of Id2 expression, thus leading to the disruption of the fusion events mediating osteoclastogenesis.
酸化チタンナノチューブ構造制御による新しい生体活性型インプラントの開発

矢谷博文, 江草宏, 関野徹

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的萌芽研究

Institution: 大阪大学

2009 - 2010

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近年、インプラント材料にナノテクノロジーを応用し、従来にない新しい機能性を見出そうとする研究が盛んにすすめられている。材料のナノ化およびその均質な形状の付与は、新規機能を発揮する可能性を秘めている。本研究の目的は、チタン表面に安価・容易な方法で「酸化チタンナノチューブ(TNT)」を合成し、この構造制御によって、新規インプラント材料の開発に結びつけることである。本研究計画の初年度には、低温化学合成法の条件検討から、高度な縦横比を示すチューブ構造のTNT層で覆われたチタン表面の作製に成功している。本年度は、TNTがCa^<2+>あるいはPO_4^<3->に対して高い吸着能を有することを明らかにした。また、0.1-1ppmのTNT粉末は、ラット骨髄由来間葉系幹細胞(rMSC)に対して著明な細胞毒性を示さず、骨芽細胞分化の指標であるアルカリフォスファターゼ活性を促進するという知見を得ている。したがって、TNT粉末は生体適合性を有した材料であり、さらに骨芽細胞分化を促進する作用を有する可能性が示唆される。さらに,TNTプレート表面にrMSCを播種して骨芽細胞への分化誘導を行った結果,チタンプレートを用いた場合と比較して、TNTプレート表面にはより多くのCaよびPが検出され、rMSCが細胞外基質の石灰化を示す骨芽細胞に効率的に分化することが明らかとなった。このように、TNTプレートはアパタイト成分吸着能を有し、培養細胞に対して細胞接着、増殖、分化誘導能を示す。したがって、TNT表面を有するチタン材料は、骨伝導能を有する医療材料として有用である可能性が示唆された。今後、TNTの構造面などを、より生体内での骨伝導に適した条件に改良にすることで、従来のチタンインプラント材料の骨伝導能を上回る性質を付与できることが期待される。
Induction of Bone Regeneration by Controlled Release of Osteogenesis/Angiogenesis Factors from Titanium Implants

YATANI Hirofumi, EGUSA Hiroshi, TABATA Yasuhiko, TABATA Yasuhiko

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Osaka University

2007 - 2009

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The aim of this study is to establish a novel technology to achieve efficient bone regeneration by controlled release of bioactive factors from titanium implants. We designed a titanium implant chamber which contains a biodegradable material releasing bioactive factor (b-FGF). We found that this system induces bone regeneration around the implant in an animal model. We also identified osteogenesis inducible peptide and small molecule, and elucidated mechanisms of these molecules on bone regeneration.
咀嚼筋・舌組織の再生に向けた筋系細胞分化促進技術の開発

江草宏, 矢谷博文

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 萌芽研究

Institution: 大阪大学

2007 - 2008

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本研究課題は,「伸展・圧縮などの外的刺激」と従来の「組織工学的手法」を組み合わせ,間葉系幹細胞をin vitroで成熟した筋管細胞・筋繊維に分化誘導し,in vivoでの咀嚼筋・舌組織再生に応用することを目的とする。筋芽細胞株C2Cl2細胞に対してカスタムメイドのバイオリアクター(反復伸展装置)を用いて継続的伸展刺激を与えた結果,細胞は一方向に配列し,筋関連遺伝子・蛋白の発現を亢進した結果,筋管細胞への分化誘導が促進された。この伸展刺激による細胞配向・筋分化促進作用を,多分化能を有する骨髄間質細胞に応用するため,マウス骨髄間質細胞よりクローナルな細胞株を樹立し,この細胞株に継続的伸展刺激を与えた。その結果,これらの細胞は一方向に配向したうえで著明に筋芽細胞へ分化することが明らかとなった。これまでの報告から,間葉系幹細胞を骨格筋系へ分化誘導することは,骨芽細胞や脂肪細胞への分化誘導と比較して困難と考えられている。我々の実験結果は,生体内における骨格筋組織の力学的環境を試験管内で擬似的に再現し,収縮・伸展を与えることで,間葉系幹細胞を骨格筋系へ分化誘導できる可能性を示唆するものである。さらに,我々はこの反復伸展刺激により分化誘導した筋系細胞を層状に重ね,細胞シートを作製することに成功した。今後,この配向細胞シートが生体内の筋組織により速やかに生着するか否かを動物実験により検討していき,舌・咀嚼筋を含めた筋組織工学に繋げたいと考えている。
5‐HTT遺伝子型および認知行動療法を用いた難治性TMD患者のスクリーニング

矢谷博文, 江草宏, 石垣尚一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 萌芽研究

Institution: 大阪大学

2007 - 2008

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顎関節症(Temporomandibular disorders: TMD)患者において,その治療効果が個人によって異なる原因のひとつとして,「ストレス処理効率の個人差」が挙げられる。本研究では,遺伝子型などの違いがこの治療効果の相違に関連している可能性を検討すること目的とし,心理学的ストレスモデルの構成から,情動ストレス,5-HTT遺伝子型,およびTMDの3者の関係についての考察を試みた。TMD患者および健常者における情動ストレス反応を,質問紙法および唾液中のストレスマーカー蛋白をELISA法で測定することによって分析を行った。TMD治療には認知行動療法を用いた。また,被験者における5-HTT遺伝子型を頬粘膜細胞由来DNAを用いてPCR法で解析する方法を確立した。ロジスティック回帰分析によりTMDの寄与因子を検討したところ,抽出された寄与因子は,唾液中コルチゾール濃度,ストレス対処行動点数,SCL-90-R身体花,SCL-90-R不安であった。これらの結果から,ストレスコーピングが不良であること,心理学的ストレス反応が強いこと,唾液中コルチゾール濃度が高いことがTMD罹患の寄与因子であることが示唆された。また,5-HTT遺伝子型の検討については,研究に協力していただける被験者が分析可能な人数に達していないため,この遺伝子型の相違がTMDに影響を及ぼすことを示唆する結果は得られていない。今後,引き続き同様の調査により,被験者数を増やし,これらの関連を検討していく予定である。
Effects of Mechanical Stress on Oral Mucosa and Its Relation to Osteo-Immunology in Alveolar Bone

EGUSA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (A)

Institution: Osaka University

2006 - 2008
合成ペプチドを用いた骨/血管新生誘導型インプラント材料の開発

矢谷博文, 高橋純造, 濱田吉野輔, 江草宏

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 萌芽研究

Institution: 大阪大学

2005 - 2006

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本研究は,歯科インプラント治療における骨増生に有用な生体材料の開発を目的とし,オステオポンチン由来のペプチドフラグメントである,Ser-Val-Val-Tyr-Gly-L.eu-Arg(SVVYGLR)に着目した。移植片が本来の骨へ置換され新生骨を形成するためには,血管新生を伴うことが必要不可欠である。研究分担者の濱田らは,これまでにSVVYGLRが血管新生作用を有することを報告してきた。本研究において,我々はSVVYGLRペプチドを合成し,歯周組織内に存在する細胞に対して生理活性を有するか否かを検討した。骨髄由来間葉系幹細胞,歯根膜細胞,歯肉線維芽細胞,歯髄細胞,血管内皮細胞を用意し,このペプチドがこれらの細胞の接着,増殖,移動能に及ぼす影響を評価した。この結果,SVVYGLRはこれらの細胞の接着能を濃度依存的に促進し,骨髄由来間葉系幹細胞,歯根膜細胞,歯髄細胞の増殖能を濃度依存的に促進した。また,SVVYGLRは歯肉線維芽細包の細胞移動能を促進した。以上の結果より,SVVYGLRは歯周組織の細胞に生理活性を有することが示唆された(国際歯科学会2007で発表予定)。また,SVVYGLRペプチドを含浸コーティシグした炭酸アパタイトコラーゲンスポンジをラット大腿骨骨髄に埋入し,1週間後に移植片の組織切片を観察したところ,スポンジ内には多数の間葉系細胞の侵入が観察され,著明な血管新生が観察された。一方,ペプチド無しのコントロール群ではこの様な像は観察されなかった(Dent Mater J, in press)。この材料については,「問葉系細胞増殖促進剤およびそれを含有する骨格系生体材料」として特許を出願した(特願2006-236970)。以上の結果から,合成ペプチドSVVYGLRをスキャフォールドと組み合わせることにより,顎骨再生に有用な生体材料を作製できる可能性が示唆された。
Study on Mechanisms of Gene Silencing during Adult Stem Cell Differentiation

YATANI Hirofumi, EGUSA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Osaka University

2004 - 2006

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We carried out this research project in collaboration with Professor Ichiro Nishimura in UCLA School of Dentistry. We reported that mouse bone marrow stromal stem cells (BMSCs) in vitro expresses a broad range of gene clusters characteristic of the advanced phenotypes of the diverse potential endpoints, and guided differentiation employ a unique conversion from broad to specific phenotype expression through silencing of discordant gene clusters (J Biol Chem 2005.). In addition, we used a custom-made extracellular matrix-related gene-focused microarray to characterize the gene expression signatures of uninduced and osteogenically-induced human BMSCs and adipose-tissue-derived stromal cells (ASCs). We found that BMSCs and ASCs both downregulate discrete ECMG sets during osteogenic differentiation, suggesting that osteogenically differentiating BMSCs and ASCs transition away from a diverse gene expression pattern reflecting their multipotency toward a configuration specifically meeting the requirements of the target lineage. This change may serve to normalize gene expression in mixed populations of stem cells derived from different tissues (Tissue Engineering, revision). We hypothesized that this transcriptional downregulation during adult stem cell differentiation may involve epigenetic events such as DNA methylation. To test this hypothesis, we carried out restriction landmark genomic scanning (RLGS) method to detect changes in methylation status within CpG islands on genomic DNA from uninduced or osteogenically-induced BMSCs. As a result, approximately 1% of RLGS spots changed their presence, implying methylation status of some CpG sites changed during osteogenic differentiation. We further analyzed CpG methylation status in osteocalcin gene promoter region of uninduced or osteogenically-induced BMSCs by bisulfite PCR method. The sequence data showed that methylation status of some specific CpG sites was altered during osteogenic differentiation. These results suggest that osteogenic differentiation of BMSCs downregulates sets of gene expressions, and DNA methylation and/or demethylation is involved in part in the gene silencing during the differentiation.
義歯性口内炎・口腔カンジダ症の新規予防法および治療法の開発 Competitive
歯槽骨の再生医療に向けた顎堤のバイオサイエンスの確立 Competitive
歯肉組織を用いたiPS細胞の樹立・分化機構に関する研究 Competitive
Differentiation mechanism of adult stem cell Competitive

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日本補綴歯科学会指導医
Board Certified Prosthodontist of the Japan Prosthodontic Society
Board Certified Infection Control Doctor of the Japanese Association for Oral Infectious Diseases
Fellow of the Royal College of Pathologists (FRCPath)
Board Certified Member of the Japanese Society for Regenerative Medicine
Infection Control Doctor (Certified by the Japanese College of Infection Control Doctors)

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