Details of the Researcher

PHOTO

Shun Hashimoto
Section
Graduate School of Life Sciences
Job title
Assistant Professor
Degree
e-Rad No.
00913980

Professional Memberships 2

  • 日本植物学会

  • 植物微生物研究会

Research Interests 11

  • メタン酸化細菌

  • 共生窒素固定

  • ペプチド

  • 抗体

  • 植物微生物相互作用

  • 植物内生菌（エンドファイト）

  • イネ

  • マメ科植物

  • 窒素固定

  • 根粒菌

  • 根粒共生

Research Areas 3

  • Life sciences / Plants: molecular biology and physiology /

  • Life sciences / Molecular biology /

  • Life sciences / Plant nutrition, soil science /

Awards 5

  1. Poster Presentation Award

    2024/01　6th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　The molecular interaction between Lotus japonicus and Bradyrhizobium mediated by type 3 effector protein, NopM

  2. Most Valuable Paper in the year 2022

    2023/08

  3. Most Valuable Paper in the year 2020

    2021/08

  4. Poster award

    2019　5th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　Mechanism of rice endophytic bradyrhizobial cell differentiation and its role on nitrogen fixation

  5. ポスター賞

    2016　第40回 蛋白質と酵素の構造と機能に関する九州シンポジウム　CCAP法による新規抗体薬物複合体の作製とその腫瘍細胞成長阻害活性評価

Papers 12

  1. The Type III Effector NopM from Bradyrhizobium elkanii USDA61 Induces a Hypersensitive Response in Lotus japonicus Root Nodules. Peer-reviewed

    Cui Ying, Satomi Nozawa, Shohei Kusakabe, Pongpan Songwattana, Pongdet Piromyou, Pakpoom Boonchuen, Panlada Tittabutr, Nantakorn Boonkerd, Hisayuki Mitsui, Shusei Sato, Neung Teaumroong, Shun Hashimoto

    Microbes and environments　40　(4)　2025/10

    DOI： 10.1264/jsme2.ME25020 　

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    Leguminous plants establish root nodule symbiosis, which is initiated by the recognition of rhizobial nodulation factors by plant receptor kinases. However, other factors, such as Type III effector proteins, also affect host specificity. We herein investigated the role of nodulation outer protein M (NopM), a Type III effector of Bradyrhizobium elkanii USDA61, in symbiosis with Lotus japonicus MG-20 and Lotus burttii. NopM, annotated as an E3 ubiquitin ligase, triggers an early senescence-like response, inducing brown nodules that hinder effective symbiosis. NopM shares structural features with E3 ubiquitin ligases derived from both pathogenic and symbiotic bacteria, including a leucine-rich-repeat and E3 ubiquitin ligase domain. The deletion of these domains or substitution of the cysteine residue, predicted to be the active site of the ubiquitin ligase domain, suppressed the formation of brown nodules. These results suggest that NopM interacts with target proteins through its leucine-rich-repeat domain and mediates ubiquitination via its ligase domain, thereby contributing to the induction of brown nodules. A transcriptome ana-lysis further suggested that the early senescence-like response closely resembled the plant hypersensitive response, with the up-regulation of defense-related genes. Therefore, L. japonicus may recognize NopM in infected nodule cells, leading to an immune response that disrupts symbiosis. The present study provides insights into the mole-cular mechanisms by which rhizobial effectors modulate symbiotic interactions in infected nodule cells, highlighting the ability of L. japonicus to activate immune responses even in nodule cells where rhizobia have been accepted.

  2. Diversity of bradyrhizobial T3SS systems and their roles in symbiosis with peanut (Arachis hypogaea) and Vigna species (V. radiata and V. mungo). International-journal Peer-reviewed

    Tarnee Phimphong, Shun Hashimoto, Pongpan Songwattana, Jenjira Wongdee, Teerana Greetatorn, Kamonluck Teamtisong, Pakpoom Boonchuen, Sachiko Masuda, Arisa Shibata, Ken Shirasu, Phoutthasone Sibounnavong, Panlada Tittabutr, Nantakorn Boonkerd, Shusei Sato, Djamel Gully, Eric Giraud, Pongdet Piromyou, Neung Teaumroong

    Applied and environmental microbiology　91　(9)　e0060025　2025/09/17

    DOI： 10.1128/aem.00600-25 　

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    Symbiosis between Bradyrhizobium strains isolated from Lao People's Democratic Republic (Lao PDR) and intercropped legumes (Arachis hypogaea, Vigna radiata, and V. mungo) was regulated by the type III secretion system (T3SS), which delivers effector proteins (T3Es) into host plant cells to modulate nodulation. To explore this mechanism, we sequenced and analyzed seven Bradyrhizobium genomes, identifying putative T3Es across five T3SS groups (G.1-G.5), which were classified based on the sequence of rhcN, a conserved ATPase gene essential for T3SS function. Phylogenetic analysis of rhcN more closely reflected the evolutionary relationships of nodulation genes than those based on 16S rRNA or whole-genome comparisons, underscoring its symbiotic relevance. Functional assays using rhcN mutants revealed group-specific effects on nodulation; G.1 strains showed neutral effects on A. hypogaea, negative effects on V. radiata, and positive effects on V. mungo. G.2 strains consistently promoted nodulation across all hosts and lacked effectors related to SUMO (small ubiquitin-like modifier) pathways, which have been implicated in host defense regulation. G.3 strains reduced nodulation in A. hypogaea but enhanced it in Vigna species. G.4 strains suppressed nodulation in A. hypogaea, and G.5 strains inhibited nodulation across all tested legumes. These findings highlight the diversity in T3SS organization, effector composition, and symbiotic responses among native Bradyrhizobium strains. The identification of known and uncharacterized effectors suggests roles in host compatibility and specificity. These strains, along with their effector profiles, provide a foundation for future functional studies to better understand T3SS-mediated interactions and support the development of targeted inoculants for legume hosts.IMPORTANCEThis study advances our understanding of legume-Bradyrhizobium symbiosis by examining the genetic organization and evolutionary patterns of T3SS genes. Our findings revealed that T3SS gene evolution does not always align with phylogenies based on 16S rRNA or whole-genome sequences, suggesting that horizontal gene transfer and functional adaptation may shape diversification. The observed variation in T3SS architecture and effector profiles among the five distinct Bradyrhizobium groups was correlated with host-specific nodulation outcomes in A. hypogaea, V. radiata, and V. mungo. We also identified novel candidate genes influencing symbiotic signaling and compatibility. These insights into the diversity and function of T3SS components contribute to a broader understanding of host-microbe communication and may support the development of more targeted and efficient rhizobial inoculants for sustainable legume cultivation and improved biological nitrogen fixation.

  3. Expanding the Eco-collection of Methane-oxidizing Bacteria Inhabiting Rice Roots: Cultivation, Isolation, and Genomic Characterization of Isolates. Peer-reviewed

    Fumika Oe, Rina Shinjo, Sachiko Masuda, Arisa Shibata, Ken Shirasu, Shun Hashimoto, Hisayuki Mitsui, Shusei Sato, Takeshi Watanabe, Susumu Asakawa

    Microbes and environments　40　(4)　2025/09

    DOI： 10.1264/jsme2.ME25012 　

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    Flooded rice fields are a major source of atmospheric methane, a strong greenhouse gas second only to carbon dioxide. Rice roots are one of the most important hotspots for methane oxidation in rice fields. However, limited information is available on the physiological and genomic characteristics of methane-oxidizing bacteria (MOB) inhabiting rice roots. In the present study, we isolated MOB from rice roots and characterized the strains phenotypically and genomically. We obtained 100 MOB-enriched cultures from the roots of three rice cultivars (Oryza sativa L. subsp. japonica cv. Nipponbare, O. sativa L. subsp. indica cv. Muha, and Tupa 121-3), in which twelve MOB isolates, two Methylomonas sp., three Methylocystis sp., and seven Methylosinus sp., were successfully purified. They showed different morphological features (types of flagellation) and colony formation potentials within the same group in some cases. A genome sequencing ana-lysis revealed variations in the number of genes or the clusters of methane monooxygenase, methanol dehydrogenase, and nitrogenase. The number of plasmid DNAs also differed among the strains. Four strains belonging to the genus Methylomonas or Methylocystis represented putative novel species based on their phenotypic and genotypic characteristics. The present study largely expanded the eco-collection of MOB cultures inhabiting rice fields and rice roots.

  4. Elucidation of the symbiotic incompatibility mechanisms between Vigna radiata and Bradyrhizobium vignae ORS3257 mediated by nodulation outer protein P2. International-journal Peer-reviewed

    Pongpan Songwattana, Pakpoom Boonchuen, Natcha Pruksametanan, Kamonluck Teamtisong, Shusei Sato, Shun Hashimoto, Nahoko Higashitani, Yasuyuki Kawaharada, Masato Araragi, Shin Okazaki, Pongdet Piromyou, Jenjira Wongdee, Teerana Greetatorn, Eric Giraud, Nantakorn Boonkerd, Panlada Tittabutr, Neung Teaumroong

    iScience　28　(5)　112351-112351　2025/05/16

    DOI： 10.1016/j.isci.2025.112351 　

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    Bradyrhizobium vignae ORS3257 is an efficient symbiotic strain for Vigna unguiculata and V. mungo but fails with V. radiata due to an effector-triggered immunity response mediated by the nodulation outer protein P2 (NopP2). To understand this incompatibility, we identified NopP2 interacting proteins in V. radiata cv. KPS1, including enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), monodehydroascorbate reductase (MDHAR), and serine hydroxymethyltransferase (SHMT) as targets. Protein-protein interaction assays confirmed that NopP2 binds to these enzymes, and further analysis revealed their co-localization on the plasma membrane. Comparative transcriptomic analysis revealed NopP2 stimulates genes related to plant defense response (PR1, PR5, MYB13, and TAO1), hydrogen peroxide (SOD, POX10, and POX16), and cell wall lignification (LAC). NopP2 did not alter the expression of genes encoding the target enzymes but interfered with MDHAR activity, leading to high H2O2 accumulation in roots. These findings suggest that NopP2 contributes to symbiotic incompatibility in V. radiata by inducing a multifaceted defense response and initiating cell wall lignification early in infection.

  5. Phenolic Acids Induce Nod Factor Production in Lotus japonicus-Mesorhizobium Symbiosis. Peer-reviewed

    Masayuki Shimamura, Takashi Kumaki, Shun Hashimoto, Kazuhiko Saeki, Shin-Ichi Ayabe, Atsushi Higashitani, Tomoyoshi Akashi, Shusei Sato, Toshio Aoki

    Microbes and environments　37　(1)　2022

    DOI： 10.1264/jsme2.ME21094 　

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    In legume-rhizobia symbiosis, partner recognition and the initiation of symbiosis processes require the mutual exchange of chemical signals. Chemicals, generally (iso)flavonoids, in the root exudates of the host plant induce the expression of nod genes in rhizobia, and, thus, are called nod gene inducers. The expression of nod genes leads to the production of lipochitooligosaccharides (LCOs) called Nod factors. Natural nod gene inducer(s) in Lotus japonicus-Mesorhizobium symbiosis remain unknown. Therefore, we developed an LCO detection method based on ultra-high-performance liquid chromatography-tandem-quadrupole mass spectrometry (UPLC-TQMS) to identify these inducers and used it herein to screen 40 phenolic compounds and aldonic acids for their ability to induce LCOs in Mesorhizobium japonicum MAFF303099. We identified five phenolic acids with LCO-inducing activities, including p-coumaric, caffeic, and ferulic acids. The induced LCOs caused root hair deformation, and nodule numbers in L. japonicus inoculated with M. japonicum were increased by these phenolic acids. The three phenolic acids listed above induced the expression of the nodA, nodB, and ttsI genes in a strain harboring a multicopy plasmid encoding NodD1, but not that encoding NodD2. The presence of p-coumaric and ferulic acids in the root exudates of L. japonicus was confirmed by UPLC-TQMS, and the induction of ttsI::lacZ in the strain harboring the nodD1 plasmid was detected in the rhizosphere of L. japonicus. Based on these results, we propose that phenolic acids are a novel type of nod gene inducer in L. japonicus-Mesorhizobium symbiosis.

  6. Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus Peer-reviewed

    Mitsutaka Fukudome, Yuta Shimokawa, Shun Hashimoto, Yusuke Maesako, Nahoko Uchi-Fukudome, Kota Niihara, Ken-ichi Osuki, Toshiki Uchiumi

    Microbes and Environments　36　(3)　n/a-n/a　2021

    Publisher: Japanese Society of Microbial Ecology

    DOI： 10.1264/jsme2.me21038 　

    ISSN： 1342-6311

    eISSN： 1347-4405

  7. Novel Anti-CD70 Antibody Drug Conjugate for the Treatment of Adult T-Cell Leukemia (ATL) International-journal Peer-reviewed

    RIRI YOKOTA, SHUN HASHIMOTO, IKUKO WATANABE, SATOSHI KISHIMOTO, MASAAKI TOYAMA, MIKA OKAMOTO, MAKOTO YOSHIMITSU, KENJI ISHITSUKA, YUJI ITO, MASANORI BABA

    Anticancer Research　40　(8)　4471-4479　2020/08

    Publisher: Anticancer Research USA Inc.

    DOI： 10.21873/anticanres.14452 　

    ISSN： 0250-7005

    eISSN： 1791-7530

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    BACKGROUND/AIM: Adult T-cell leukemia (ATL) is a hematological malignancy caused by infection with human T-cell leukemia virus type 1 (HTLV-1). Chemotherapy, antibody therapy, and bone marrow transplantation are used to treat this disease, however, median survival time has not been significantly improved. Our aim was to develop and evaluate a novel antibody-drug conjugate (ADC) with regards to cell cytotoxicity and target specificity. MATERIALS AND METHODS: In this study, we have constructed a novel ADC, which is composed of an anti-CD70 single chain Fv-Fc antibody conjugated with the anticancer agent emtansine using a novel antibody modification method. Cell cytotoxicity and target specificity were assessed using a cell proliferation assay. RESULTS: The anti-CD70 ADC selectively killed HTLV-1-infected cells and ATL cells without affecting other cells. CONCLUSION: The anti-CD70 ADC offers some chemotherapeutic potential for the treatment of ATL.

  8. Mechanisms of Rice Endophytic Bradyrhizobial Cell Differentiation and Its Role in Nitrogen Fixation Peer-reviewed

    Teerana Greetatorn, Shun Hashimoto, Taro Maeda, Mitsutaka Fukudome, Pongdet Piromyou, Kamonluck Teamtisong, Panlada Tittabutr, Nantakorn Boonkerd, Masayoshi Kawaguchi, Toshiki Uchiumi, Neung Teaumroong

    Microbes and Environments　35　(3)　n/a-n/a　2020/07/29

    Publisher: Japanese Society of Microbial Ecology

    DOI： 10.1264/jsme2.me20049 　

    ISSN： 1342-6311

    eISSN： 1347-4405

  9. Type III Secretion System of Bradyrhizobium sp. SUTN9-2 Obstructs Symbiosis with Lotus spp. Peer-reviewed

    Shun Hashimoto, Kohki Goto, Pongdet Pyromyou, Pongpan Songwattana, Teerana Greetatorn, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong, Toshiki Uchiumi

    Microbes and Environments　35　(3)　n/a-n/a　2020/07/02

    Publisher: Japanese Society of Microbial Ecology

    DOI： 10.1264/jsme2.me20041 　

    ISSN： 1342-6311

    eISSN： 1347-4405

  10. Homocitrate Synthase Genes of Two Wide-Host-Range Bradyrhizobium Strains are Differently Required for Symbiosis Depending on Host Plants Peer-reviewed

    Shun Hashimoto, Jenjira Wongdee, Pongpan Songwattana, Teerana Greetatorn, Kohki Goto, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong, Toshiki Uchiumi

    Microbes and Environments　34　(4)　393-401　2019/10/08

    Publisher: Japanese Society of Microbial Ecology

    DOI： 10.1264/jsme2.me19078 　

    ISSN： 1342-6311

    eISSN： 1347-4405

  11. Empowering rice seedling growth by endophytic Bradyrhizobium sp. SUTN 9‐2 Peer-reviewed

    T. Greetatorn, S. Hashimoto, S. Sarapat, P. Tittabutr, N. Boonkerd, T. Uchiumi, N. Teaumroong

    Letters in Applied Microbiology　2019/02/06

    Publisher: Wiley

    DOI： 10.1111/lam.13114 　

    ISSN： 0266-8254

    eISSN： 1472-765X

  12. Gene expression and localization of a β-1,3-glucanase of Lotus japonicus Peer-reviewed

    Ken-ichi Osuki, Shun Hashimoto, Akihiro Suzuki, Masato Araragi, Akihito Takahara, Makiko Kurosawa, Ken-ichi Kucho, Shiro Higashi, Mikiko Abe, Toshiki Uchiumi

    Journal of Plant Research　129　(4)　749-758　2016/07

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10265-016-0811-6 　

    ISSN： 0918-9440

    eISSN： 1618-0860

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Presentations 41

  1. NosZを保有するクローバー根粒菌の単離とN2O削減効果の検証

    原田智彦, 鈴木涼太, 橋本駿, 番場大, 羽山ちさと, 河村柚保, 鮫島玲子, 佐藤修正

    植物微生物研究会 第34回研究交流会　2025/09

  2. 根粒菌のⅢ型エフェクターに起因するミヤコグサの根粒共生制御

    橋本駿, 番場大, 日下部翔平, Yusdar Mustamin, 金子貴一, 岡崎伸, 三井久幸, 佐藤修正

    植物微生物研究会 第34回研究交流会　2025/09

  3. ミヤコグサ特異的な新規フラボノイド化合物の根粒形成促進効果

    金子純士, 橋本駿, 明石智義, 佐藤修正

    植物微生物研究会 第34回研究交流会　2025/09

  4. ミヤコグサの形質転換根を活用したBradyrhizobium elkanii USDA61のⅢ型エフェクターNopMの機能解析

    野澤里美, Cui Ying, 番場大, 花野滋, 三井久幸, 佐藤修正, 橋本駿

    植物微生物研究会 第34回研究交流会　2025/09

  5. 根粒菌エフェクターに起因するミヤコグサの根粒共生制御機構の解析

    橋本 駿, 番場 大, 日下部 翔平, Yusdar Mustamin, 高澤 瑞希, Ying Cui, Piromyou Pongde, Songwattana Pongpan, Tittabutr Panlada, Boonkerd Nantakorn, Teaumroong Neung, 金子 貴一, 岡崎 伸, 内海 俊樹, 三井 久幸, 佐藤 修正

    日本植物学会 第88回大会　2024/09/16

  6. 根粒菌エフェクターに起因するミヤコグサの根粒共生制御に関わる遺伝子の探索

    橋本 駿, 番場 大, 日下部 翔平, Yusdar Mustamin, 高澤 瑞希, Ying Cui, Piromyou Pongde, Songwattana Pongpan, Tittabutr Panlada, Boonkerd Nantakorn, Teaumroong Neung, 金子 貴一, 岡崎 伸, 内海 俊樹, 三井 久幸, 佐藤 修正

    植物微生物研究会 第33回研究交流会　2024/08/28

  7. 地表根が塩害水田でのイネ減収軽減に及ぼすメカニズム解明に向けた土壌環境計測

    半澤栄子, 番場大, 橋本駿, 大場義之, 佐藤雅志, 木富悠花, 河合翼, 常田岳志, 佐藤修正, 宇賀優作

    日本育種学会第145回講演会　2024/03

  8. ミヤコグサPINK4遺伝子の機能と細胞内共生成立後の根粒菌共生制御

    嵐田遥, 中川知己, 橋本駿, 番場大, Yusdar mustamin, 三井久幸, 佐伯和彦, 川口正代司, 佐藤修正

    第65回 日本植物生理学会年会　2024/03

  9. The molecular interaction between Lotus japonicus and Bradyrhizobium mediated by type 3 effector protein, NopM

    Cui Ying, Shun Hashimoto, Pongpan Songwattana, Pongdet Piromyou, Natcha Pruksametanan, Pakpoom Boonchuen, Panlada Tittabutr, Hisayuki Mitsui, Neung Teaumroong, Shusei Sato

    6th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　2024/01/09

  10. Plant root endophytic bacteria for mitigation of greenhouse gas from agricultural fields

    Shun Hashimoto, Masaru Bamba, Hajime Ozawa, Argen Adem Abdela, Sawa Hara, Atsuo Suzuki, Hisayuki Mitsui, Kiwamu Minamisawa, Shusei Sato

    6th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　2024/01/08

  11. Updated information on legume experimental resources in NBRP Lotus/Glycine

    Shusei Sato, Shun Hashimoto, Masaru Bamba, Yusdar Mustamin, Turgut Akyol, Stig Andersen, Takuyu Hashiguchi, Masatsugu Hashiguchi

    6th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　2024/01/08

  12. Nod-factor independent symbiotic phenomena and symbiotic inhibition factor of Bradyrhizobium and Aeschynomene genus

    Shogo Fukunaga, Shun Hashimoto, Shusei Sato, Shin Okazaki

    6th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　2024/01

  13. Molecular Basis of Mung Bean Symbiotic Incompatibility: A Pull-Down Assay Identifies Plant Proteins Targeted by NopP2 from Bradyrhizobium sp. ORS3257

    Pongpan Songwattana, Pakpoom Boonchuen, Natcha Pruksametanan, Shun Hashimoto, Pongdet Piromyou, Jenjira Wongdee, Teerana Greetatorn, Kamonluck Teamtisong, Sato Shusei, Panlada Tittabutr, Nantakorn Boonkerd, Eric Giraud, Neung Teaumroong

    6th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　2024/01

  14. イネにおけるメタン酸化微生物コンソーシアムの探索と分離

    新庄莉奈, 大江史花, Ma Xuping, 福嶋大智, 梶浦雅子, 常田岳志, 橋本駿, 三井久幸, 佐藤修正, 渡邉健史, 浅川晋

    植物微生物研究会 第32回研究交流会　2023/09

  15. イネ細胞質型グルタミン合成酵素1;2 (OsGS1;2) が根の微生物群集に与える影響の解析

    小澤元, 番場大, 橋本駿, 佐藤修正, 小島創一

    日本植物学会 第87回大会　2023/09

  16. ミヤコグサPINK4遺伝子を介した細胞内共生成立後の根粒菌共生制御

    嵐田 遥, 中川 知己, 橋本駿, 番場大, Yusdar Mustamin, 三井 久幸, 佐藤 修正

    植物微生物研究会 第32回研究交流会　2023/09

  17. Expanding the Eco Collection of Methane Oxidizing Bacteria from Rice Roots.

    Fumika Oe, Rina Shinjo, Sachiko Masuda, Shun Hashimoto, Hisayuki Mitsui, Shusei Sato, Takeshi, Watanabe, Susumu Asakawa

    日本地球惑星科学連合大会, 2023.　2023/05

  18. クサネム根粒菌Bradyrhizobium sp. SUTN9-2のⅢ型分泌系はミヤコグサとの共生窒素固定を阻害する

    Shun Hashimoto, Kohki Goto, Pongdet Pyromyou, Pongpan Songwattana, Teerana Greetatorn, Masahiro Fukuda, Cui Ying, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong, Toshiki Uchiumi, Shusei Sato

    第64回 日本植物生理学会年会　2023/03/15

  19. クサネム根粒菌Bradyrhizobium sp. SUTN9-2のⅢ型分泌系はミヤコグサとの共生を妨げる

    橋本 駿, 後藤 滉己, Pongdet Pyromyou, Pongpan Songwattana, Teerana Greetatorn, 福田 将大, Cui Ying, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong, 内海 俊樹, 佐藤修正

    植物微生物研究会 第31回研究交流会　2022/09/09

  20. 水田由来メタン酸化細菌エココレクションの充実

    大江史花, 新庄莉奈, 橋本駿, 三井久幸, 佐藤修正, 渡邉健史, 浅川晋

    第35回日本微生物生態学会　2022

  21. 低メタン排出イネ品種における⾼活性メタン酸化細菌群の探索

    新庄莉奈, 大江史花, Ma Xuping, 福嶋大智, 梶浦雅子, 常田岳志, 橋本駿, 三井久幸, 佐藤修正, 渡邉健史, 浅川晋

    第35回日本微生物生態学会　2022

  22. Bradyrhizobium sp. SUTN9-2のbclA遺伝子を破壊するとクサネムとの共生窒素固定能が向上する

    橋本 駿, Teerana Greetatorn, Pongpan Songwattana, Pongdet Piromyou, 福田 将大, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong, 佐藤 修正, 内海 俊樹

    植物微生物研究会 第30回研究交流会　2021/09/09

  23. Mechanism of rice endophytic bradyrhizobial cell differentiation and its role on nitrogen fixation

    Greetatorn T, Hashimoto S, Tittabutr P, Uchiumi T, Teaumroong N

    5th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　2019

  24. Contribution of rhizobial nifV genes to symbiosis between two wide-host-range Bradyrhizobium strains and their various host plants

    Hashimoto S, Wongdee J, Songwattana P, Greetatorn T, Goto K, Tittabutr P, Teaumroong N, Uchiumi T

    5th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　2019

  25. Mechanism of rice endophytic bradyrhizobial cell differentiation and its role on nitrogen fixation

    Greetatorn T, Hashimoto S, Tittabutr P, Uchiumi T, Teaumroong N

    第28回 植物微生物研究会　2018

  26. Bradyrhizobium属根粒菌のnifVは自由生活下および根粒共生中での窒素固定に関与する

    橋本駿, Wongdee J, Greetatorn T, Songwattann P, 後藤滉己, Giraud E, Tittabutr P, Teaumroong N, 内海俊樹

    第28回 植物微生物研究会　2018

  27. NifV 遺伝子を破壊した広宿主域根粒菌の多様な宿主植物との共生

    後藤滉己, 橋本駿, Greetatorn T, Songwattana P, Tittaburt P, Teaumroong N, 九町健一, 内海俊樹

    日本動物学会九州支部（第71回）・九州沖縄植物学会（第68回）・日本生態学会九州地区会（第63回）・三学会合同宮崎大会　2018

  28. A novel covalently-labeling method of IgG by using protein A-mimic peptide

    Himeno A, Nakashima Y, Yatoumaru R, Hashimoto S, Kamrul Hasan K, Kato D, Ito Y

    第54回ペプチド討論会　2017

  29. 広宿主域根粒菌のbacA及びnifV遺伝子の破壊が多様な宿主植物との共生に及ぼす影響

    橋本駿, Greetatorn T, Songwattana P, 後藤滉己, Tittaburt P, Teaumroong N, 九町健一, 内海俊樹

    第27回 植物微生物研究会　2017

  30. Mechanism of rice endophytic bradyrhizobial differentiation and its role on nitrogen fixation

    Teaumroong N, Greetatorn T, Hashimoto S, Tittaburt P, Boonkerd N, Uchiumi T

    20th International congress on nitrogen fixation　2017

  31. Conjugation of human IgG with HSA by a site-specific modification, CCAP method

    Nakashima Y, Himeno A, Yatoumaru R, Hashimoto S, Tanaka A, Kato D, Ito Y

    2th Antibody Society Korea summer workshop　2017

  32. CCAP法を用いたヒトIgG抗体への部位特異的HSA付加による抗体検出感度の向上

    中島洋介, 姫野ありさ, 八藤丸亮, 橋本駿, 田中亜澄, 加藤太一郎, 伊東祐二

    平成29年度日本生化学会九州支部例会　2017

  33. 抗体薬物複合体の作製を目指したCCAP法の開発と作製したADCの機能評価

    横田璃里, 橋本駿, 辻井温子, 加藤太一郎, 馬場昌範, 伊東祐二

    第23回 日本生物工学会九州支部 飯塚大会　2016

  34. 新規ヒトIgG部位特異的修飾技術を用いたIn-111標識トラスツズマブの性能評価

    中田徳仁, 正山祥生, 林明希男, 辻井温子, 橋本駿, 伊東祐二

    第31回日本薬物動態学会　2016

  35. 抗体薬物複合体の創製に向けた癌細胞特異的単鎖Fv-Fcの修飾法

    前田祐加, 榎元友理恵, 吉川大和, 橋本駿, 辻井温子, 加藤太一郎, 伊東祐二

    第89回日本生化学会大会　2016

  36. CCAP法による新規抗体薬物複合体の作製とその腫瘍細胞成長阻害活性評価

    横田璃里, 前田祐加, 橋本駿, 辻井温子, 加藤太一郎, 馬場昌範, 伊東祐二

    第40回 蛋白質と酵素の構造と機能に関する九州シンポジウム　2016

  37. CCAP法による抗体薬物複合体の作製とその機能評価

    横田璃里, 前田祐加, 橋本駿, 辻井温子, 加藤太一郎, 伊東祐二

    平成28年度日本生化学会九州支部例会　2016

  38. Gene expression and localization of a β-1,3-glucanase of Lotus japonicus

    Osuki K, Suzuki A, Takahara A, Araragi M, Hashimoto S, Iwasaki N, Kucho K, Higashi S, Abe M, Uchiumi T

    18th International Congress on Nitrogen Fixation　2013

  39. Characterization of LjGlu1, β-1,3-glucanase gene of Lotus japonicus

    Hashimoto S, Araragi M, Takahara A, Suzuki A, Kucho K, Abe M, Higashi S, Uchiumi T

    2th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation　2012

  40. ミヤコグサのアブシシン酸応答性β-1,3-glucanaseの組換えタンパク質の生産.

    橋本駿, 鈴木章弘, 九町健一, 阿部美紀子, 東四郎, 内海俊樹

    日本動物学会九州支部（第64回）・日本植物学会九州支部（第61回）・日本生態学会九州地区会（第56回）・合同長崎大会,　2011/04

  41. ミヤコグサのβ-1,3-glucanase遺伝子の特性

    橋本駿, 高原皓史, 鈴木章弘, 九町健一, 阿部美紀子, 東四郎, 内海俊樹

    第21回植物微生物研究会　2011

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Research Projects 1

  1. 根粒菌エフェクターに起因するマメ科植物の根粒共生制御機構

    橋本 駿

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 若手研究

    Institution: 東北大学

    2025/04/01 - 2028/03/31

Social Activities 2

  1. 第64回 日本植物生理学会年会 高校生発表 企画担当（仙台年会）

    2023/03/15 - 2023/03/17

  2. 高校生徒理科研究発表会（講評および審査）

    2020/11/04 -