Details of the Researcher

PHOTO

Takuya Minokawa
Section
Graduate School of Life Sciences
Job title
Associate Professor
Degree
  • 博士(理学) (The University of Tokyo)

Research History 3

  • 2001/02 - 2005/01
    Division of Biology, California Institute of Technology Postdoctoral Scholar in Biology

  • 2000/05 - 2001/02
    Graduate School of Frontier Sciences, the University of Tokyo Visiting Research Associate

  • 1997/10 - 2000/04
    Department of Life Science, Tokyo Institute of Technology Research Associate

Education 2

  • The University of Tokyo Graduate School, Division of Science 生物科学専攻

    - 1997/09

  • Hirosaki University Faculty of Science 生物学科

    - 1992/03

Committee Memberships 2

  • 日本動物学会 男女共同参画委員会 委員

    2016/10 - 2019/09

  • 日本動物学会 キャリアパス小委員会 委員

    2016/10 - 2019/09

Professional Memberships 1

  • The Zoological Society of Japan

Research Interests 2

  • 進化

  • 個体発生

Research Areas 2

  • Life sciences / Evolutionary biology /

  • Life sciences / Developmental biology /

Awards 1

  1. 生命科学研究科研究奨励賞

    2005/11/08 東北大学大学院生命科学研究科 「形態と生活史の多様性を生み出す個体発生機構の進化に関する研究」

Papers 39

  1. Records of the galeommatoid bivalve Nipponomysella subtruncata (Yokoyama, 1927), a commensal with the sipunculan Siphonosoma cumanense (Keferstein, 1867) from Miyagi and Aomori Prefectures, Japan

    (34) 109-114 2023/03

  2. An easy and rapid staining method for confocal microscopic observation and reconstruction of three‐dimensional images of echinoderm larvae and juveniles Peer-reviewed

    Kohei Tokanai, Yasuhiro Kamei, Takuya Minokawa

    Development, Growth & Differentiation 63 (9) 478-487 2021/12

    Publisher: Wiley

    DOI: 10.1111/dgd.12758  

    ISSN: 0012-1592

    eISSN: 1440-169X

  3. Meiobenthic Polychaete Dinophilus sp. cf. gyrociliatus (Annelida: Dinophilidae) from Japan with SEM Observation and DNA Barcodes Peer-reviewed

    Naoto Jimi, Takuya Minokawa, Toru Miura, Hiroshi Kajihara

    Species Diversity 25 (2) 213-218 2020/09/18

    Publisher: Japanese Society of Systematic Zoology

    DOI: 10.12782/specdiv.25.213  

    ISSN: 1342-1670

    eISSN: 2189-7301

  4. Pmar1/phb homeobox genes and the evolution of the double-negative gate for endomesoderm specification in echinoderms. International-journal Peer-reviewed

    Atsuko Yamazaki, Yoshiaki Morino, Makoto Urata, Masaaki Yamaguchi, Takuya Minokawa, Ryohei Furukawa, Mariko Kondo, Hiroshi Wada

    Development (Cambridge, England) 2020/01/30

    DOI: 10.1242/dev.182139  

    More details Close

    In several model animals, the earliest phases of embryogenesis are regulated by lineage-specific genes, such as Drosophila bicoid Sea urchin (echinoid) embryogenesis is initiated by zygotic expression of pmar1, a paired-class homeobox gene that has been considered to be present only in the lineage of modern urchins (euechinoids). In euechinoids, Pmar1 promotes endomesoderm specification by repressing the hairy and enhancer of split C (hesC) gene. Here, we identified the basal echinoid (cidaroid) pmar1 gene, which also promotes endomesoderm specification but not by repressing hesC A further search for related genes demonstrated that other echinoderms have pmar1-related genes named phb Functional analyses of starfish Phb proteins indicated that similar to cidaroid Pmar1, they promote activation of endomesoderm regulatory gene orthologs via an unknown repressor that is not HesC. Based on these results, we propose that Pmar1 may have recapitulated the regulatory function of Phb during the early diversification of echinoids and that the additional repressor HesC was placed under the control of Pmar1 in the euechinoid lineage. This case provides an exceptional model for understanding how early developmental processes diverge.

  5. Cidaroids, clypeasteroids, and spatangoids: Procurement, culture, and basic methods Invited Peer-reviewed

    Hibino T, Minokawa T, Yamazaki A

    Methods in Cell Biology 150 81-103 2019

  6. Development of the coelomic cavities in larvae of the living isocrinid sea lily Metacrinus rotundus Peer-reviewed

    Amemiya S, Hibino T, Minokawa T, Naruse K, Kamei Y, Uemura I, Kiyomoto M, Hisanaga S, Kuraishi R

    Acta Zool. 100 (E-Pub) 414-430 2018/09

    Publisher: Wiley

    DOI: 10.1111/azo.12274  

    ISSN: 0001-7272

    eISSN: 1463-6395

  7. Anteroposterior molecular registries in ectoderm of the echinus rudiment Peer-reviewed

    Adachi S, Niimi I, Sakai Y, Sato F, Minokawa T, Urata M, Sehara-Fujisawa A, Kobayashi I, Yamaguchi M

    Developmental Dynamics 247 (12) 1297-1307 2018

    Publisher: Wiley

    DOI: 10.1002/dvdy.24686  

    ISSN: 1058-8388

  8. Comparative studies on the skeletogenic mesenchyme of echinoids Invited Peer-reviewed

    Takuya Minokawa

    DEVELOPMENTAL BIOLOGY 427 (2) 212-218 2017/07

    DOI: 10.1016/j.ydbio.2016.11.011  

    ISSN: 0012-1606

    eISSN: 1095-564X

  9. Characterization of paramyosin and thin filaments in the smooth muscle of acorn worm, a member of hemichordates Peer-reviewed

    Hiroki Sonobe, Takashi Obinata, Takuya Minokawa, Tomohiro Haruta, Yuuki Kawamura, Shinryu Wakatsuki, Naruki Sato

    JOURNAL OF BIOCHEMISTRY 160 (6) 369-379 2016/12

    DOI: 10.1093/jb/mvw047  

    ISSN: 0021-924X

    eISSN: 1756-2651

  10. Roles of hesC and gcm in echinoid larval mesenchyme cell development Peer-reviewed

    Atsuko Yamazaki, Takuya Minokawa

    DEVELOPMENT GROWTH & DIFFERENTIATION 58 (3) 315-326 2016/04

    DOI: 10.1111/dgd.12277  

    ISSN: 0012-1592

    eISSN: 1440-169X

  11. Early stalked stages in ontogeny of the living isocrinid sea lily Metacrinus rotundus Peer-reviewed

    Shonan Amemiya, Akihito Omori, Toko Tsurugaya, Taku Hibino, Masaaki Yamaguchi, Ritsu Kuraishi, Masato Kiyomoto, Takuya Minokawa

    ACTA ZOOLOGICA 97 (1) 102-116 2016/01

    DOI: 10.1111/azo.12109  

    ISSN: 0001-7272

    eISSN: 1463-6395

  12. Neurogenesis in directly and indirectly developing enteropneusts: of nets and cords Peer-reviewed

    Sabrina Kaul-Strehlow, Makoto Urata, Takuya Minokawa, Thomas Stach, Andreas Wanninger

    ORGANISMS DIVERSITY & EVOLUTION 15 (2) 405-422 2015/06

    DOI: 10.1007/s13127-015-0201-2  

    ISSN: 1439-6092

    eISSN: 1618-1077

  13. Expession patterns of mesenchyme specification genes in two distantly related echinoids, Glyptocidaris crenularis and Echinocardium cordatum Peer-reviewed

    Atsuko Yamazaki, Takuya Minokawa

    GENE EXPRESSION PATTERNS 17 (2) 87-97 2015/03

    DOI: 10.1016/j.gep.2015.03.003  

    ISSN: 1567-133X

    eISSN: 1872-7298

  14. Larval mesenchyme cell specification in the primitive echinoid occurs independently of the double-negative gate Peer-reviewed

    Atsuko Yamazaki, Yumi Kidachi, Masaaki Yamaguchi, Takuya Minokawa

    DEVELOPMENT 141 (13) 2669-2679 2014/07

    DOI: 10.1242/dev.104331  

    ISSN: 0950-1991

    eISSN: 1477-9129

  15. "Micromere" formation and expression of endomesoderm regulatory genes during embryogenesis of the primitive echinoid Prionocidaris baculosa Peer-reviewed

    Atsuko Yamazaki, Yumi Kidachi, Takuya Minokawa

    DEVELOPMENT GROWTH & DIFFERENTIATION 54 (5) 566-578 2012/06

    DOI: 10.1111/j.1440-169X.2012.01360.x  

    ISSN: 0012-1592

  16. First record of Balanoglossus misakiensis Kuwano, 1902 (Phylum HEMICHORDATA) in Mutsu Bay, Aomori Prefecture

    阿部広和, 鷲尾正彦, 山嵜敦子, 美濃川拓哉, 西川輝昭

    青森自然誌研究 2012

  17. Role of the Nanos Homolog During Sea Urchin Development Peer-reviewed

    Takayoshi Fujii, Naoaki Sakamoto, Hiroshi Ochiai, Kazumasa Fujita, Yuka Okamitsu, Noriko Sumiyoshi, Takuya Minokawa, Takashi Yamamoto

    DEVELOPMENTAL DYNAMICS 238 (10) 2511-2521 2009/10

    DOI: 10.1002/dvdy.22074  

    ISSN: 1058-8388

  18. Evolutionary modification of T-brain (tbr) expression patterns in sand dollar Peer-reviewed

    Keiko Minemura, Masaaki Yamaguchi, Takuya Minokawa

    GENE EXPRESSION PATTERNS 9 (7) 468-474 2009/10

    DOI: 10.1016/j.gep.2009.07.008  

    ISSN: 1567-133X

  19. Evolutionary modification of specification for the endomesoderm in the direct developing echinoid Peronella japonica: loss of the endomesoderm-inducing signal originating from micromeres Peer-reviewed

    Minoru Iijima, Yasuhiro Ishizuka, Yoko Nakajima, Shonan Amemiya, Takuya Minokawa

    DEVELOPMENT GENES AND EVOLUTION 219 (5) 235-247 2009/05

    DOI: 10.1007/s00427-009-0286-8  

    ISSN: 0949-944X

  20. Expression patterns of wnt8 orthologs in two sand dollar species with different developmental modes Peer-reviewed

    Hidewo Nakata, Takuya Minokawa

    GENE EXPRESSION PATTERNS 9 (3) 152-157 2009/03

    DOI: 10.1016/j.gep.2008.11.004  

    ISSN: 1567-133X

  21. cis-regulatory inputs of the wnt8 gene in the sea urchin endomesoderm network Peer-reviewed

    T Minokawa, AH Wikramanayake, EH Davidson

    DEVELOPMENTAL BIOLOGY 288 (2) 545-558 2005/12

    DOI: 10.1016/j.ydbio.2005.09.047  

    ISSN: 0012-1606

  22. Molecular heterotopy in the expression of Brachyury orthologs in order Clypeasteroida (irregular sea urchins) and order Echinoida (regular sea urchins) Peer-reviewed

    T Hibino, Y Harada, T Minokawa, M Nonaka, S Amemiya

    DEVELOPMENT GENES AND EVOLUTION 214 (11) 546-558 2004/11

    DOI: 10.1007/s00427-004-0437-x  

    ISSN: 0949-944X

  23. R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres Peer-reviewed

    R Revilla-i-Domingo, T Minokawa, EH Davidson

    DEVELOPMENTAL BIOLOGY 274 (2) 438-451 2004/10

    DOI: 10.1016/j.ydbio.2004.07.008  

    ISSN: 0012-1606

  24. SpHnf6, a transcription factor that executes multiple functions in sea urchin embryogenesis Peer-reviewed

    O Otim, G Amore, T Minokawa, DR McClay, EH Davidson

    DEVELOPMENTAL BIOLOGY 273 (2) 226-243 2004/09

    DOI: 10.1016/j.ydbio.2004.05.033  

    ISSN: 0012-1606

  25. Expression patterns of four different regulatory genes that function during sea urchin development Peer-reviewed

    T Minokawa, JP Rast, C Arenas-Mena, CB Franco, EH Davidson

    GENE EXPRESSION PATTERNS 4 (4) 449-456 2004/07

    DOI: 10.1016/j.modgep.2004.01.009  

    ISSN: 1567-133X

  26. Blastomere isolation and transplantation Invited

    Hyla Sweet, Shonan Amemiya, Andrew Ransick, Takuya Minokawa, David R. McClay, Athula Wikramanayake, Ritsu Kuraishi, Masato Kiyomoto, Hiroki Nishida, Jonathan Henry

    Methods in Cell Biology 2004 (74) 243-271 2004

    ISSN: 0091-679X

  27. Genetic divergence of two local Japanese populations of the Echinothurioid echinoid, Asthenosoma ijimai

    Takuya Minokawa, Shonan Amemiya, Norimasa Matsuoka

    Bull. Fac. Agric. & Life Sci. Hirosaki Univ. 5 1-8 2003

    Publisher: Hirosaki University

    ISSN: 1344-8897

  28. A provisional regulatory gene network for specification of endomesoderm in the sea urchin embryo Peer-reviewed

    EH Davidson, JP Rast, P Oliveri, A Ransick, C Calestani, CH Yuh, T Minokawa, G Amore, Hinman, V, C Arenas-Mena, O Otim, CT Brown, CB Livi, PY Lee, R Revilla, MJ Schilstra, PJC Clarke, AG Rust, ZJ Pan, MI Arnone, L Rowen, RA Cameron, DR McClay, L Hood, H Bolouri

    DEVELOPMENTAL BIOLOGY 246 (1) 162-190 2002/06

    DOI: 10.1006/dbio.2002.0635  

    ISSN: 0012-1606

  29. New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization Peer-reviewed

    A Ransick, JP Rast, T Minokawa, C Calestani, EH Davidson

    DEVELOPMENTAL BIOLOGY 246 (1) 132-147 2002/06

    DOI: 10.1006/dbio.2002.0607  

    ISSN: 0012-1606

  30. A genomic regulatory network for development Peer-reviewed

    EH Davidson, JP Rast, P Oliveri, A Ransick, C Calestani, CH Yuh, T Minokawa, G Amore, Hinman, V, C Arenas-Mena, O Otim, CT Brown, CB Livi, PY Lee, R Revilla, AG Rust, ZJ Pan, MJ Schilstra, PJC Clarke, MI Arnone, L Rowen, RA Cameron, DR McClay, L Hood, H Bolouri

    SCIENCE 295 (5560) 1669-1678 2002/03

    DOI: 10.1126/science.1069883  

    ISSN: 0036-8075

    eISSN: 1095-9203

  31. Large-scale cDNA analysis of the maternal genetic information in the egg of Halocynthia roretzi for a gene expression catalog of ascidian development Peer-reviewed

    KW Makabe, T Kawashima, S Kawashima, T Minokawa, A Adachi, H Kawamura, H Ishikawa, R Yasuda, H Yamamoto, K Kondoh, S Arioka, Y Sasakura, A Kobayashi, K Yagi, K Shojima, Y Kondoh, S Kido, M Tsujinami, N Nishimura, M Takahashi, T Nakamura, M Kanehisa, M Ogasawara, T Nishikata, H Nishida

    DEVELOPMENT 128 (13) 2555-2567 2001/07

    ISSN: 0950-1991

  32. Binary specification of nerve cord and notochord cell fates in ascidian embryos Peer-reviewed

    T Minokawa, K Yagi, KW Makabe, H Nishida

    DEVELOPMENT 128 (11) 2007-2017 2001/06

    ISSN: 0950-1991

  33. A large-scale whole-mount in situ hybridization system: Rapid one-tube preparation of DIG-labeled RNA probes and high throughput hybridization using 96-well silent screen plates Peer-reviewed

    M Ogasawara, T Minokawa, Y Sasakura, H Nishida, KW Makabe

    ZOOLOGICAL SCIENCE 18 (2) 187-193 2001/03

    DOI: 10.2108/zsj.18.187  

    ISSN: 0289-0003

  34. Micromere descendants at the blastula stage are involved in normal archenteron formation in sea urchin embryos Peer-reviewed

    Y Ishizuka, T Minokawa, S Amemiya

    DEVELOPMENT GENES AND EVOLUTION 211 (2) 83-88 2001/02

    DOI: 10.1007/s004270000120  

    ISSN: 0949-944X

  35. Large-scale cDNA analysis of the maternal genetic information in the egg of the ascidian, Halocynthia roretzi, for the gene expression catalog during development. Peer-reviewed

    Makabe, K. W, Kawashima, T, Kawashima, S, Minokawa, T, Adachi, A, Kawamura, H, Ishikawa, H, Yasuda, R, Yamamoto, H, Kondoh, K, Arioka, S, Sasakura, Y, Kobayashi, A, Yagi, K, Shojima, K, Kondoh, Y, Kido, S, Tsujinami, M, Nishimura, N, Takahashi, M, Nakamura, T, Kanehisa, M, Ogasawara, M, Nishikata, T, Nishida, H

    Development, 128, 2555-2567 2001/01

  36. Studies on the potential of micromeres to induce archenteron differentiation in embryos of a direct-developing sand dollar, Peronella japonica

    M Iijima, Y Ishizuka, T Minokawa, S Amemiya

    ZYGOTE 8 S80-S80 2000

    ISSN: 0967-1994

  37. Timing of the potential of micromere-descendants in echinoid embryos to induce endoderm differentiation of mesomere-descendants Peer-reviewed

    T Minokawa, S Amemiya

    DEVELOPMENT GROWTH & DIFFERENTIATION 41 (5) 535-547 1999/10

    DOI: 10.1046/j.1440-169X.1999.00453.x  

    ISSN: 0012-1592

  38. Mesodermal cell differentiation in echinoid embryos derived from the animal cap recombined with a quartet of micromeres Peer-reviewed

    T Minokawa, S Amemiya

    ZOOLOGICAL SCIENCE 15 (4) 541-545 1998/08

    DOI: 10.2108/zsj.15.541  

    ISSN: 0289-0003

  39. Skeletogenic potential of induced secondary mesenchyme cells derived from the presumptive ectoderm in echinoid embryos Peer-reviewed

    T Minokawa, Y Hamaguchi, S Amemiya

    DEVELOPMENT GENES AND EVOLUTION 206 (7) 472-476 1997/03

    ISSN: 0949-944X

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Misc. 4

  1. ゲノムからみたウニの特徴

    美濃川拓哉

    生物の科学 遺伝 61 (4) 2007/07/01

    Publisher: エヌ・ティー・エス

  2. A large-scale whole-mount in situ hybridization system: Rapid one-tube preparation of DIG-labeled RNA probes and high throughput hybridization using 96-well silent screen plates (vol 18, pg 187, 2001)

    M Ogasawara, T Minokawa, Y Sasakura, H Nishida, KW Makabe

    ZOOLOGICAL SCIENCE 18 (3) 443-443 2001/04

    ISSN: 0289-0003

  3. MECHANISM OF BINARY SPECIFICATION OF NOTOCHORD AND NERVE CORD CELL FATES IN ASCIDIAN EMBRYOS(Developmental Biology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan :

    Minokawa T., Nishida H.

    Zoological science 17 82-82 2000

    Publisher: Zoological Society of Japan

    ISSN: 0289-0003

  4. A CONVENIENT METHOD OF WHOLE MOUNT IN SITU HYBRIDIZATION (WMISH) FOR COMPREHENSIVE SURVEY OF MULTIPLE cDNA CLONES(Developmental Biology)(Proceedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan) :

    Minokawa T., Ogasawara M., Sasakura Y., Yamamoto H., Nishida H., Makabe K. W.

    Zoological science 15 65-65 1998

    Publisher: Zoological Society of Japan

    ISSN: 0289-0003

Books and Other Publications 3

  1. Atlas of marine invertebrate larvae

    Hibino, Taku, Minokawa, Takuya, Amemiya, Shonan (Editors, Boyle, Michael J., Young, Craig M., Sewell, Mary

    Academic Press, an imprint of Elsevier,Array 2025/04

    ISBN: 9780081028711

  2. Sea Urchins: Biology, Fishery, and Paleontology

    Takuya Minokawa

    Tokai University Press 2009/03/05

    ISBN: 9784486018100

  3. Methods in Cell Biology Vol. 74

    H. Sweet, S. Amemiya, A. Ransick, T. Minokawa, D.R. McClay, A. Wikramanayake, R. Kuraishi, M. Kiyomoto, H. Nishida, J. Henry

    Elsevier Inc. 2004/12

Presentations 10

  1. Evolution of the micromeres in echinoids International-presentation

    Commemorative Symposium for the 27th International Prize for Biology "Genetic Regulation of Development" 2011/11/30

  2. 青森湾に生息するギボ シムシ類(半索動物腸 鰓綱)

    浦田慎, 西川輝昭, 阿部広和, 鷲尾正彦

    日本動物学会第82回大会 2011/09/21

  3. ウニ類の「祖先型」幼生 骨片形成機構

    山嵜敦子, 木立由美

    日本動物学会第82回大会 2011/09/21

  4. ウニにおける第一卵割 面と幼生前後軸の関係 の多様性

    雨宮昭南

    日本動物学会第82回大会 2011/09/21

  5. 直接発生ウニ・ヨツアナカシパンの発生メカニズム

    第6回 棘皮動物研究集会 2009/12/12

  6. ウニの発生遺伝子ネットワーク研究の現在と将来

    日本動物学会第78回大会 2007/09/20

  7. Using developmental diversity to study evolution of developmental mechanism International-presentation

    The Developmental Biology of the Sea Urchin XVII 2006/09/27

  8. 直接発生型ウニをモデルに発生メカニズムの進化を探る

    日本動物学会第77回大会 2006/09/21

  9. ウニ初期発生メカニズムのゲノム情報を利用した解析

    日本発生生物学会第38回大会 2005/06/01

  10. Cis-regulatory control of SpWnt8 International-presentation

    The Developmental Biology of the Sea Urchin. XVI 2005/04

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Research Projects 7

  1. 発生機構多様性に関する進化発生生物学的研究 Competitive

    System: The Other Research Programs

    2005/02 - Present

  2. 間隙棲環形動物に学ぶ生物多様性実習:教材開発と実践

    美濃川 拓哉

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2023/04 - 2026/03

  3. 海産クマムシ学への飼育実験系によるアプローチ

    藤本 心太, 美濃川 拓哉

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 山口大学

    2022/04/01 - 2025/03/31

  4. Study on the post-embryonic development of the echinoids

    Minokawa Takuya

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04 - 2021/03

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    The present study deals the questions related to post-embryonic development, such as what is a larva, what is an adult, and what is the mechanism that forms an adult. We focus on the following two topics: [1] Formation process and structure of adult rudiment in normal echinoid development, and [2] Understanding the mechanical relationship between embryonic and post-embryonic development using the regeneration experimental system. For [1], We have discovered a new feature in the morphological change of the digestive tract during the metamorphosis period from larva to adult. Regarding [2], it was verified whether or not the larval constituent cells are involved in post-embryonic development using a larval regeneration experimental system. It was confirmed that the larvae can regenerate the missing part and reconstruct the functionally and structurally normal digestive tract even if most of the digestive tract is removed experimentally.

  5. Mechanism of the reorganization of developmental gene regulatory network

    MINOKAWA Takuya, YAMAZAKI Atsuko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2010 - 2012

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    The purpose of the present research is to elucidate the molecular mechanism responsible for the gene regulatory network (GRN) reorganization during animal evolution. Various species belonging to the ambulacraria have been used as model species. Especially, we are interested in tbr expression in the apical organ of the sand dollar Peronella japonica. We confirmed that P. japonica is an exceptional echinoid species that possesses tbr expression in the apical organ. The cis-regulatory analysis for P. japonica tbr revealed that approximately 1 kb upstream sequence of the coding region suffices to activate transcription of a reporter gene in the broad regions containing mesoderm and ectoderm.

  6. Evolution of the micromere gene network in sand dollars

    YAMAGUCHI Masaaki, MINOKAWA Takuya, WADA Hiroshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Kanazawa University

    2009 - 2011

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    It is considered that, in echinoids, indirect development is the ancestral mode, and direct development has evolved independently in several lineages. To examine modifications of molecular mechanisms that drive the evolution of direct development, I analyzed expression patterns of the micromere specification genes in two sand dollars with distinct developmental modes. Between the two species, early expression patterns of the regulatory genes were largely conserved. After the mid blastula stage, however, a unique domain emerged in the direct developer, where either delta or hesC is not expressed. Since this region gives rise to mesenchyme cells which will form coelomic compartments, this modification may promote formation of adult structures in the direct developer.

  7. An evolutionary study on the transcriptional regulation mechanism in the context of the structural evolution of cis-regulatory region

    MINOKAWA Takuya

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2006 - 2008

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Social Activities 4

  1. 平内町立東平内中学校「職場訪問」

    2005/10/04 - 2005/10/05

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    ウニの発生観察と棘皮動物の解剖実習を指導。浅虫海洋生物学研究センターの業務と研究設備を紹介。

  2. 浅虫水族館ジュニアクラブ

    2015/12 -

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    浅虫水族館ジュニアクラブに参加する小学生を対象に、顕微鏡によるウニの発生観察を指導した。

  3. 筑波大学 マリンバイオ先端科学セミナー

    2006/12/20 -

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    「ポストウニゲノム時代の棘皮動物発生学」と題して、ウニゲノム研究および新口動物進化発生学の現在と今後の展望について講演した。

  4. 生命科学研究科春季講演会

    2006/04/22 -

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    「オタマジャクシを経ないカエル、プルテウス幼生を経ないウニ -進化発生学への招待-」と題して、進化発生学についての講演を行った。

Other 6

  1. 箒虫動物の実験動物化と進化発生学研究基盤の確立

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    本申請の研究は、進化研究上重要な意義があるにもかかわらず、研究の進んでいない箒虫(ホウキムシ)動物を実験動物化し、その進化発生学研究を立ち上げることを目標としている。 ホウキムシ類は2属11種からなる小さな動物群である。近年の分子系統解析によって、箒虫動物は旧口動物のなかでも冠輪動物に属すると位置付けられたが、個体発生上の形質には旧口動物的特徴に加えて、新口動物との類縁性を示唆する形質が数多く見られる。たとえば、卵割、初期割球の調節能、原腸陥入様式、中胚葉形成様式、幼生(アクチノトロカ幼生)の体腔構成において、新口動物(特に水腔動物)との類似性が示唆されている(異論も多い)。旧口動物であるホウキムシ類が有する新口動物的な発生学的特徴は、収斂進化により獲得されたものである可能性が高い。それゆえ、形態や発生機構の収斂を研究するための好適な対象であり、後生動物進化のより深い理解に貢献する可能性がある。しかしながら、これまでのホウキムシ発生学の成果の多くは個体発生過程の記載が中心であり、しかもその数は少ない。特に細胞分化や形態形成のメカニズムとその進化についてはほとんど何もわかっていない。 私のホウキムシ研究の究極目標は、ホウキムシの進化発生生物学を立ち上げることにある。本申請の研究はその基盤となるホウキムシの実験動物化を目指している。フィールド調査による幼生および成体の生活史のさらなる理解に加え、海洋から採集した浮遊幼生の実験室内飼育技術を開発し、実験室内で全生活史を全うさせる完全実験動物化を目指す。申請者は青森県陸奥湾でのプランクトンネット採集で複数種類の幼生を頻繁に得ており、これらをもちいて予備飼育実験を進めている。これまでに一部の幼生を成体まで発生させることに成功した。幼生を実験室内で性成熟した成体まで飼育し、次世代を得ることができれば、進化発生学上極めて興味深い動物が現代的生物学の俎上に載ることになる。

  2. 半索動物ギボシムシの神経発生に関する研究

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    半索動物ギボシムシの神経発生過程を詳細に研究し、その知見をウニ類などの他の新口動物と比較することによって、神経発生メカニズムの進化機構を明らかにする。

  3. 半索動物ギボシムシの筋肉構成分子に関する研究

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    半索動物ギボシムシの筋肉構成分子のレパートリーを解析し、その結果を原始的棘皮動物トリノアシを含む他の新口動物と比較することによって、筋肉構成分子の進化機構を明らかにする。

  4. 発生様式の多様化に関わる遺伝子ネットワークの研究

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    直接発生型ウニ・ヨツアナカシパンの発生調節遺伝子のネットワークを研究することで、間接発生型(祖先型)ウニからどのようにして直接発生型発生様式が進化したのかを解明する。

  5. 原始的棘皮動物トリノアシの個体発生に関する研究

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    原始的棘皮動物トリノアシの個体発生過程を詳細に研究し、その知見をウニ類などの他の棘皮動物と比較することによって、発生メカニズムの進化機構を明らかにする。

  6. 直接発生型ウニの発生遺伝子ネットワークの研究

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    典型的な発生をするウニ(間接発生型ウニ)から比較的最近進化した直接発生型ウニの発生遺伝子ネットワークを研究することで、発生メカニズムの進化につながったゲノムの変化を解明する。

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