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Satoru Tsuda

Section

Graduate School of Medicine

Job title

Associate Professor

Degree

博士（医学）（東北大学）

researchmap

https://researchmap.jp/stsuda

J-GLOBAL ID

201801009742324074

Research History 8

2016/10 - Present

Tohoku university hospital　Ophthalmology　Assistant Professor
2015/04 - 2016/09

Pharmaceuticals and Medical Devices Agency　Office of new drug　Reviewer
2012 - 2016

Graduate school of medicine, Tohoku university　Department of Ophthalmology　Graduate student
2011/06 - 2012/03

Yamagata City Hospital SAISEIKAN　Ophthalmology　Medical director
2010/04 - 2011/06

Tohoku university hospital　Ophthalmology　Clinical fellow
2009/04 - 2010/03

Tohoku university hospital　Ophthalmology　Senior resident
2007/04 - 2009/03

Tohoku university hospital　Junior Redisent
2001/04 - 2007/03

Tohoku university school of medicine　Student

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Professional Memberships 3

日本眼循環学会
JAPAN GLAUCOMA SOCIETY
JAPANESE OPHTHALMOLOGICAL SOCIETY

Research Interests 6

Pharmaceuticals and Medical Devices Development
Real-time imaging
Retinal neuroprotective therapy
Filtration surgery
Retinal diseases
Glaucoma

Research Areas 2

Life sciences / Ophthalmology / Glaucoma, Retinal diseases
Life sciences / Pathobiochemistry / Ocular diseases

Awards 3

優秀演題賞

2023/07　日本眼循環学会
Young Researcher's Award

2018/04　Miyagi Ophthalmologists Association
須田記念緑内障治療研究奨励基金

日本緑内障学会　カルパインを標的とする緑内障の神経保護治療の開発

Papers 146

Evaluation of blood flow in arteritic anterior ischemic optic neuropathy using laser speckle flowgraphy: A case series

Chiaki Yamaguchi, Naoki Kiyota, Noriko Himori, Takahiro Oshima, Takayuki Takeshita, Kazuko Omodaka, Satoru Tsuda, Toru Nakazawa

American Journal of Ophthalmology Case Reports　38　2025/06

DOI： 10.1016/j.ajoc.2025.102316 　

ISSN： 2451-9936
Detection of Glaucomatous Visual Fields Using a Newly Developed Device with Retinal Projection Technology.

Satoru Tsuda, Hiroshi Kunikata, Makoto Ishikawa, Airi Takagi, Takeshi Yabana, Mitsuru Suzuki, Mitsuru Sugawara, Seiji Morino, Toru Nakazawa

The Tohoku journal of experimental medicine　2025/02/27

DOI： 10.1620/tjem.2025.J032 　
A hybrid multi model artificial intelligence approach for glaucoma screening using fundus images. International-journal

Parmanand Sharma, Naoki Takahashi, Takahiro Ninomiya, Masataka Sato, Takehiro Miya, Satoru Tsuda, Toru Nakazawa

NPJ digital medicine　8　(1)　130-130　2025/02/27

DOI： 10.1038/s41746-025-01473-w 　

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Glaucoma, a leading cause of blindness, requires accurate early detection. We present an AI-based Glaucoma Screening (AI-GS) network comprising six lightweight deep learning models (total size: 110 MB) that analyze fundus images to identify early structural signs such as optic disc cupping, hemorrhages, and nerve fiber layer defects. The segmentation of the optic cup and disc closely matches that of expert ophthalmologists. AI-GS achieved a sensitivity of 0.9352 (95% CI 0.9277-0.9435) at 95% specificity. In real-world testing, sensitivity dropped to 0.5652 (95% CI 0.5218-0.6058) at ~0.9376 specificity (95% CI 0.9174-0.9562) for the standalone binary glaucoma classification model, whereas the full AI-GS network maintained higher sensitivity (0.8053, 95% CI 0.7704-0.8382) with good specificity (0.9112, 95% CI 0.8887-0.9356). The sub-models in AI-GS, with enhanced capabilities in detecting early glaucoma-related structural changes, drive these improvements. With low computational demands and tunable detection parameters, AI-GS promises widespread glaucoma screening, portable device integration, and improved understanding of disease progression.
Metabolomic Profiling of Open-Angle Glaucoma Etiologic Endotypes: Tohoku Multi-Omics Glaucoma Study

Akiko Hanyuda, Yoshihiko Raita, Takahiro Ninomiya, Kazuki Hashimoto, Naoko Takada, Kota Sato, Jin Inoue, Seizo Koshiba, Gen Tamiya, Akira Narita, Masato Akiyama, Kazuko Omodaka, Satoru Tsuda, Yu Yokoyama, Noriko Himori, Yasuko Yamamoto, Takazumi Taniguchi, Kazuno Negishi, Toru Nakazawa

Investigative Ophthalmology & Visual Science　65　(13)　44-44　2024/11/20
Publisher: Association for Research in Vision and Ophthalmology (ARVO)
DOI： 10.1167/iovs.65.13.44 　

ISSN： 1552-5783
羞明により生活に支障をきたしている症例に対するacunisサングラスの使用経験

福岡世理, 竹下孝之, 高橋久美子, 竹内雅史, 津田聡, 中澤徹

日本視能矯正学会プログラム抄録集　65回　56-56　2024/11
Publisher: 日本視能矯正学会
Relationship between corneal hysteresis and the site of damage to peripapillary retinal nerve fibre layer thickness in open-angle glaucoma. International-journal

Yoko Takeda, Akiko Hanyuda, Naoki Takahashi, Kazuko Omodaka, Takahiro Ninomiya, Naoki Kiyota, Satoru Tsuda, Yu Yokoyama, Noriko Himori, Airi Takagi, Toru Nakazawa

Scientific reports　14　(1)　26329-26329　2024/11/01

DOI： 10.1038/s41598-024-76187-2 　

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Corneal hysteresis (CH) is associated with glaucomatous structural changes. We retrospectively investigated the association between CH and the regional circumpapillary retinal nerve fibre layer thickness (cpRNFLT) in 419 eyes of 419 patients with normal-tension glaucoma (NTG) and primary open-angle glaucoma (POAG). CH was used as the explanatory variable, and cpRNFLT (total and quadrant) was used as the dependent variable. Standardized β coefficients were compared both overall and between the NTG and POAG groups. Multiple regression analysis with CH as the explanatory variable and cpRNFLT parameters as the dependent variables suggested that even after adjusting for age, sex, intraocular pressure (IOP), axial length, and central corneal thickness, all areas of the cpRNFLT were significantly associated with CH, except for the nasal quadrant. In the stratified analysis by glaucoma type, a positive relationship between CH and regional cpRNFLT tended to be maintained in the NTG group but not in the POAG group (P = 0.060 for interaction). Additionally, in the NTG group, the CH-inferior cpRNFLT relationship was particularly strong in the less-IOP fluctuation group. These results suggest that CH may be a potential predictor of anatomical vulnerability around the optic nerve, particularly enhancing the inferior regions of NTG.
網膜中心動脈閉塞症とカルパイン阻害薬の臨床試験

國方彦志, 津田聡, 中澤徹

臨床眼科　78　(10)　1187-1192　2024/10
Publisher: (株)医学書院
ISSN： 0370-5579

eISSN： 1882-1308
網膜中心動脈閉塞症とカルパイン阻害薬の臨床試験

國方彦志, 津田聡, 中澤徹

臨床眼科　78　(10)　1187-1192　2024/10
Publisher: (株)医学書院
ISSN： 0370-5579

eISSN： 1882-1308
網膜投影技術を用いたセルフ視野チェッカーMEOCHECKの有用性

津田聡, 石川誠, 矢花武史, 宮武博, 西村尭幸, 鈴木誠, 菅原充, 森野誠治, 横山悠, 檜森紀子, 中澤徹

日本緑内障学会抄録集　35回　83-83　2024/09
Publisher: 日本緑内障学会
Differentiating optic neuropathies using laser speckle flowgraphy: Evaluating blood flow patterns in the optic nerve head and peripapillary choroid. International-journal

Chiaki Yamaguchi, Naoki Kiyota, Noriko Himori, Kazuko Omodaka, Satoru Tsuda, Toru Nakazawa

Acta ophthalmologica　2024/08/13

DOI： 10.1111/aos.16747 　

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PURPOSE: To compare blood flow (BF) impairment patterns in different optic neuropathies using laser speckle flowgraphy (LSFG). METHODS: This retrospective study enrolled 24 eyes of 24 patients with non-arteritic anterior ischemic optic neuropathy (NAAION), 59 eyes of 59 patients with optic neuritis (ON), 677 eyes of 677 patients with open-angle glaucoma (OAG), and 110 eyes of 110 controls. The patient backgrounds of all groups were compared. Ophthalmologic findings were evaluated, adjusting for age, sex, blood pressure, pulse rate, and underlying systemic diseases with 1:1 optimal propensity score matching. We used LSFG to obtain optic nerve head (ONH) vessel-area mean blur rate (MBR; ONH-MV), ONH tissue-area MBR (ONH-MT), and choroidal MBR. The NAAION and ON groups were compared with the control and OAG groups. RESULTS: Best-corrected visual acuity was worse in the NAAION, ON, and OAG groups than in controls (p < 0.001). Circumpapillary retinal nerve fibre layer thickness was higher in the NAAION and ON groups and lower in the OAG group than in controls (p < 0.001). Compared to controls, the NAAION and OAG groups had significantly lower ONH-MV, ONH-MT, and choroidal MBR (p < 0.05). Additionally, the NAAION group had lower ONH-MV and choroidal MBR than the OAG group (p = 0.003 and p < 0.001, respectively) but no difference in ONH-MT (p = 0.857). The ON group had significantly lower ONH-MV and choroidal MBR compared to the controls (p < 0.001 and p = 0.022, respectively) but no difference in ONH-MT (p = 0.773). CONCLUSION: Optic neuropathies showed different patterns of ocular BF impairment. Therefore, LSFG can be a useful tool for differentiating optic neuropathies.
The relationship between equol production status and normal tension glaucoma. International-journal

Noriko Himori, Keiko Uchida, Takahiro Ninomiya, Masashi Nagai, Kota Sato, Satoru Tsuda, Kazuko Omodaka, Toru Nakazawa

International ophthalmology　44　(1)　287-287　2024/06/27

DOI： 10.1007/s10792-024-03225-3 　

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PURPOSE: Equol is metabolized by intestinal bacteria from soy isoflavones and is chemically similar to estrogen. Dietary habits, such as consumption of soy products, influence equol production. A relationship between glaucoma and estrogen has been identified; here, we investigated the relationship between equol production status and glaucoma in Japan. METHODS: We recruited 68 normal-tension glaucoma (NTG) patients (male to female ratio 26:42, average age 63.0 ± 7.6 years) and 31 controls (male to female ratio 13:18, average age 66.0 ± 6.3 years) from our hospital. All women included were postmenopausal. Urinary equol concentration was quantified with the ELISA method. MD was calculated based on the Humphrey visual field. The association between MD and equol was analyzed with Spearman's rank correlation coefficient. The Mann-Whitney U test was used to compare the equol-producing (> 1 μM) and non-producing (< 1 μM) subjects. We also investigated the association between equol and glaucoma with a logistic regression analysis. RESULTS: There was a significant association between equol and MD (r = 0.36, P < 0.01) in the NTG patients. Glaucoma, represented by MD, was significantly milder in the equol-producing subjects than the non-equol producing subjects (P = 0.03). A multivariate analysis revealed the independent contributions of equol, cpRNFLT, and IOP to MD (P = 0.03, P = 0.04, and P < 0.01, respectively). CONCLUSION: Our results suggest that equol, acting through estrogen receptor-mediated neuroprotective effects, might be involved in suppressing the progression of NTG. This result also adds to evidence that glaucoma may be influenced by lifestyle.
Effectiveness of remote risk-based monitoring and potential benefits for combination with direct data capture. International-journal

Osamu Yamada, Shih-Wei Chiu, Toru Nakazawa, Satoru Tsuda, Mitsuhide Yoshida, Toshifumi Asano, Taiki Kokubun, Kazuki Hashimoto, Munenori Takata, Suzuka Ikeda, Yosuke Kawabe, Yuko Tamura, Takuhiro Yamaguchi

Trials　25　(1)　384-384　2024/06/14

DOI： 10.1186/s13063-024-08242-2 　

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BACKGROUND: In recent years, alternative monitoring approaches, such as risk-based and remote monitoring techniques, have been recommended instead of traditional on-site monitoring to achieve more efficient monitoring. Remote risk-based monitoring (R2BM) is a monitoring technique that combines risk-based and remote monitoring and focuses on the detection of critical data and process errors. Direct data capture (DDC), which directly collects electronic source data, can facilitate R2BM by minimizing the extent of source documents that must be reviewed and reducing the additional workload on R2BM. In this study, we evaluated the effectiveness of R2BM and the synergistic effect of combining R2BM with DDC. METHODS: R2BM was prospectively conducted with eight participants in a randomized clinical trial using a remote monitoring system that uploaded photographs of source documents to a cloud location. Critical data and processes were verified by R2BM, and later, all were confirmed by on-site monitoring to evaluate the ability of R2BM to detect critical data and process errors and the workload of uploading photographs for clinical trial staff. In addition, the reduction of the number of uploaded photographs was evaluated by assuming that the DDC was introduced for data collection. RESULTS: Of the 4645 data points, 20.9% (n = 973, 95% confidence interval = 19.8-22.2) were identified as critical. All critical data errors corresponding to 5.4% (n = 53/973, 95% confidence interval = 4.1-7.1) of the critical data and critical process errors were detectable by R2BM. The mean number of uploaded photographs and the mean time to upload them per visit per participant were 34.4 ± 11.9 and 26.5 ± 11.8 min (mean ± standard deviation), respectively. When assuming that DDC was introduced for data collection, 45.0% (95% confidence interval = 42.2-47.9) of uploaded photographs for R2BM were reduced. CONCLUSIONS: R2BM can detect 100% of the critical data and process errors without on-site monitoring. Combining R2BM with DDC reduces the workload of R2BM and further improves its efficiency.
Comparative features of superior versus inferior hemisphere microvasculature dropout in open-angle glaucoma.

Naoki Takahashi, Kazuko Omodaka, Tsutomu Kikawa, Takahiro Ninomiya, Naoki Kiyota, Satoru Tsuda, Toru Nakazawa

Japanese journal of ophthalmology　2024/05/30

DOI： 10.1007/s10384-024-01071-5 　

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PURPOSE: This study aimed to investigate differences in microvasculature dropout (MvD) between the superior and inferior hemispheres in glaucoma patients. STUDY DESIGN: Retrospective and cross-sectional. METHODS: Fifty-eight eyes of 58 open-angle glaucoma patients (age 61.12 ± 10.19 years, mean deviation - 7.32 ± 6.36 dB) were included. MvD was detected with en face images from swept-source optical coherence tomography angiography. Blood flow at the optic nerve head was measured with laser speckle flowgraphy, represented as the mean blur rate in tissue (MBRT). Logistic and linear regression models adjusted for age, intraocular pressure, axial length, and circumpapillary retinal nerve fiber layer thickness were used to investigate the relationship between various factors and MvD angle in each hemisphere. RESULTS: The presence of inferior MvD was related to peripapillary atrophy-β area (odds ratio = 14.10 [2.49-234.00], P = 0.019). Superior MvD angle was significantly related to MBRT in the superior quadrant (β = -0.31 [- 0.60 - -0.02], P = 0.037). Inferior MvD angle was significantly related to peripapillary atrophy-β area (β = 0.49 [0.21-0.77], P = 0.001). CONCLUSIONS: Only superior MvD demonstrated a significant relationship with reduced ocular blood flow. In contrast, inferior MvD was associated with mechanical stress. These findings may suggest a potential difference in pathophysiology between superior and inferior MvD.
Sector-specific association of intraocular pressure dynamics in dark-room prone testing and visual field defect progression in glaucoma. International-journal

Masataka Sato, Naoki Kiyota, Takeshi Yabana, Shigeto Maekawa, Satoru Tsuda, Kazuko Omodaka, Noriko Himori, Yu Yokoyama, Toru Nakazawa

Ophthalmology. Glaucoma　2024/03/28

DOI： 10.1016/j.ogla.2024.03.007 　

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PURPOSE: To investigate sectoral differences in the relationship between intraocular pressure (IOP) dynamics during dark-room prone testing (DRPT) and visual field (VF) defect progression in primary open-angle glaucoma (POAG) patients. DESIGN: Retrospective, longitudinal study PARTICIPANTS: This retrospective study included 116 eyes of 84 POAG patients who underwent DRPT and had at least five reliable VF tests conducted over a more than two-year follow-up period. We excluded eyes with mean deviation worse than -20 dB or a history of intraocular surgery or laser treatment. METHODS: Average total deviation (TD) was calculated in the superior, central, and inferior sectors of the Humphrey 24-2 or 30-2 program. During DRPT, IOP was measured in the sitting position, and after 60 minutes in the prone position in a dark room, IOP was measured again. The relationship between IOP change during DRPT, IOP after DRPT, and TD slope in each quadrant was analyzed with a linear mixed-effects model, adjusting for other potential confounding factors. MAIN OUTCOME MEASURES: TD slope in each quadrant, IOP change during DRPT, and IOP after DRPT RESULTS: IOP after DRPT and IOP change during DRPT were 18.16±3.42 mmHg and 4.92±3.12 mmHg, respectively. Superior TD slope was significantly associated with both IOP after DRPT (β=-0.28, p=0.003) and IOP change during DRPT (β=-0.21, p=0.029), while central (β=-0.05, p=0.595; β=-0.05; p=0.622) and inferior (β=0.05, p=0.611; β=0.01, p=0.938) TD slopes were not. CONCLUSION: DRPT might be a useful test to predict the risk of superior VF defect progression in eyes with POAG.
Laser speckle flowgraphyを用いた視神経炎病態別の眼内血流評価について

山口知暁, 檜森紀子, 清田直樹, 大島隆寛, 木村暸, 津田聡, 面高宗子, 三須建郎, 青木正志, 中澤徹

日本眼科学会雑誌　128　(臨増)　193-193　2024/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
偏光OCTの濾過胞瘢痕組織自動抽出による術後早期での術後成績の予測

村上真之, 津田聡, 吉田真彰, 國分太貴, 山成正宏, 岡本道明, Suchandra Banarjee, 横山悠, 矢花武史, 前川重人, 檜森紀子, 中澤徹

日本眼科学会雑誌　128　(臨増)　248-248　2024/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
網膜投影技術を用いた簡易視野測定装置MEOCHECKによる白内障評価の追加解析

中島匡, 矢花武史, 佐藤正隆, 二宮高洋, 山崎舞, 津田聡, 檜森紀子, 横山悠, 森野誠治, 鈴木誠, 石川誠, 國方彦志, 中澤徹

日本眼科学会雑誌　128　(臨増)　274-274　2024/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
Laser speckle flowgraphyを用いた視神経炎病態別の眼内血流評価について

山口知暁, 檜森紀子, 清田直樹, 大島隆寛, 木村暸, 津田聡, 面高宗子, 三須建郎, 青木正志, 中澤徹

日本眼科学会雑誌　128　(臨増)　193-193　2024/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
偏光OCTの濾過胞瘢痕組織自動抽出による術後早期での術後成績の予測

村上真之, 津田聡, 吉田真彰, 國分太貴, 山成正宏, 岡本道明, Suchandra Banarjee, 横山悠, 矢花武史, 前川重人, 檜森紀子, 中澤徹

日本眼科学会雑誌　128　(臨増)　248-248　2024/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
網膜投影技術を用いた簡易視野測定装置MEOCHECKによる白内障評価の追加解析

中島匡, 矢花武史, 佐藤正隆, 二宮高洋, 山崎舞, 津田聡, 檜森紀子, 横山悠, 森野誠治, 鈴木誠, 石川誠, 國方彦志, 中澤徹

日本眼科学会雑誌　128　(臨増)　274-274　2024/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
Author Correction: Reduced glutathione level in the aqueous humor of patients with primary open-angle glaucoma and normal-tension glaucoma. International-journal

Kota Sato, Daisuke Saigusa, Taiki Kokubun, Amane Fujioka, Qiwei Feng, Ritsumi Saito, Akira Uruno, Naomi Matsukawa, Michiko Ohno-Oishi, Hiroshi Kunikata, Yu Yokoyama, Masayuki Yasuda, Noriko Himori, Kazuko Omodaka, Satoru Tsuda, Shigeto Maekawa, Masayuki Yamamoto, Toru Nakazawa

npj aging　10　(1)　8-8　2024/01/20

DOI： 10.1038/s41514-024-00137-5 　
The Effect of β-Blocker Eye Drops on Pulse Rate, Ocular Blood Flow, and Glaucoma Progression: A Retrospective Longitudinal Study. International-journal

Naoki Kiyota, Yukihiro Shiga, Takahiro Ninomiya, Satoru Tsuda, Kazuko Omodaka, Noriko Himori, Yu Yokoyama, Kyongsun Pak, Toru Nakazawa

Advances in therapy　2024/01/02

DOI： 10.1007/s12325-023-02762-0 　

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INTRODUCTION: Our study was conducted to determine factors associated with the effectiveness of a β-blocker eye drop add-on in altering pulse rate (PR) in glaucoma patients. METHODS: This retrospective study examined 236 eyes of 138 patients who received a β-blocker eye drop add-on during follow-up. Patients were included if at least one PR measurement was available both before and after the add-on was started. We collected data on ophthalmic parameters: longitudinal PR; longitudinal choroidal blood flow, represented by laser speckle flowgraphy-measured mean blur rate (MBR); and diacron-reactive oxygen metabolites (d-ROMs). We used a multivariable linear mixed-effects model to investigate the effectiveness of the β-blocker eye drop add-on in altering PR and examined factors contributing to a larger PR alteration after the add-on was started by analyzing the effect on PR of the interaction term between the add-on and clinical factors. We used the k-means method to classify the patients. RESULTS: The β-blocker eye drop add-on reduced PR (- 7.61 bpm, P < 0.001). Female gender, higher PR when the add-on was started, lower central corneal thickness, and a higher d-ROM level were associated with greater reduction in PR (P < 0.05). In a cluster of patients with these clinical features, choroidal MBR increased by + 3.42% when we adjusted for change over time; MD slope, which represents the speed of glaucoma progression, improved by + 0.64 dB/year (P < 0.05). CONCLUSIONS: We identified a glaucoma subgroup in which PR decreased, choroidal blood flow increased, and glaucoma progression slowed after a β-blocker eye drop add-on was started.
The Effect of β-Blocker Eye Drops on Pulse Rate, Ocular Blood Flow, and Glaucoma Progression: A Retrospective Longitudinal Study

Naoki Kiyota, Yukihiro Shiga, Takahiro Ninomiya, Satoru Tsuda, Kazuko Omodaka, Noriko Himori, Yu Yokoyama, Kyongsun Pak, Toru Nakazawa

Advances in Therapy　2024

DOI： 10.1007/s12325-023-02762-0 　

ISSN： 0741-238X

eISSN： 1865-8652
A ginger extract improves ocular blood flow in rats with endothelin-induced retinal blood flow dysfunction. International-journal

Nana Takahashi, Kota Sato, Naoki Kiyota, Satoru Tsuda, Namie Murayama, Toru Nakazawa

Scientific reports　13　(1)　22715-22715　2023/12/20

DOI： 10.1038/s41598-023-49598-w 　

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The aim of this study was to investigate the effect of a ginger extract on optic nerve head blood flow (ONH BF) under endothelin-1 (ET-1) stimulation. Using laser speckle flowgraphy, we measured ONH BF in brown Norway rats. To establish the ONH BF impairment profile under ET-1 stimulation, we administered an intravitreal injection of ET-1 under anesthesia. We then gave the ginger extract sublingually to assess its effect on ONH BF in both normal and ET-1-induced ischemic conditions. Post ET-1 injection, there were no significant changes in parameters including intraocular pressure or systemic factors. ONH BF showed a dose-dependent decline after ET-1 injection, with a significant reduction after a 2.50 pmol ET-1 dose. Sublingual administration of the ginger extract significantly improved ONH BF in both normal and ET-1-stimulated rats. This suggests that our newly developed supplement for improving ONH BF has a potential role in retinal ischemic diseases, including glaucoma.
Reduced glutathione level in the aqueous humor of patients with primary open-angle glaucoma and normal-tension glaucoma. International-journal

Kota Sato, Daisuke Saigusa, Taiki Kokubun, Amane Fujioka, Qiwei Feng, Ritsumi Saito, Akira Uruno, Naomi Matsukawa, Michiko Ohno-Oishi, Hiroshi Kunikata, Yu Yokoyama, Masayuki Yasuda, Noriko Himori, Kazuko Omodaka, Satoru Tsuda, Shigeto Maekawa, Masayuki Yamamoto, Toru Nakazawa

npj aging　9　(1)　28-28　2023/11/21

DOI： 10.1038/s41514-023-00124-2 　

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Glaucoma is a leading cause of blindness worldwide in older people. Profiling the aqueous humor, including the metabolites it contains, is useful to understand physiological and pathological conditions in the eye. In the current study, we used mass spectrometry (MS) to characterize the aqueous humor metabolomic profile and biological features of patients with glaucoma. Aqueous humor samples were collected during trabeculectomy surgery or cataract surgery and analyzed with global metabolomics. We included 40 patients with glaucoma (32 with POAG, 8 with NTG) and 37 control subjects in a discovery study. VIP analysis revealed five metabolites that were elevated and three metabolites that were reduced in the glaucoma patients. The identified metabolomic profile had an area under the receiver operating characteristic curve of 0.953. Among eight selected metabolites, the glutathione level was significantly decreased in association with visual field defects. Moreover, in a validation study to confirm the reproducibility of our findings, the glutathione level was reduced in NTG and POAG patients compared with a cataract control group. Our findings demonstrate that aqueous humor profiling can help to diagnose glaucoma and that various aqueous humor metabolites are correlated with clinical parameters in glaucoma patients. In addition, glutathione is clearly reduced in the aqueous humor of glaucoma patients with both IOP-dependent and IOP-independent disease subtypes. These findings indicate that antioxidant agents in the aqueous humor reflect glaucomatous optic nerve damage and that excessive oxidative stress may be involved in the pathogenesis of glaucoma.
The association between intraocular pressure dynamics during dark-room prone testing and intraocular pressure over a relatively long-term follow-up period in primary open-glaucoma patients. International-journal

Masataka Sato, Naoki Kiyota, Takeshi Yabana, Shigeto Maekawa, Satoru Tsuda, Kazuko Omodaka, Noriko Himori, Yu Yokoyama, Toru Nakazawa

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie　2023/10/21

DOI： 10.1007/s00417-023-06257-0 　

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PURPOSE: To investigate the relationship between the dynamics of intraocular pressure (IOP) during dark-room prone testing (DRPT) and IOP over a relatively long-term follow-up period. METHODS: This retrospective study enrolled 84 eyes of 51 primary open-angle glaucoma patients who underwent DRPT for whom at least three IOP measurements made using Goldmann applanation tonometry were available over a maximum follow-up period of two years. We excluded eyes with a history of intraocular surgery or laser treatment and those with changes in topical anti-glaucoma medication during the follow-up period. In DRPT, IOP was measured in the sitting position, and after 60 min in the prone position in a dark room, IOP was measured again. In this study, IOP fluctuation refers to the standard deviation (SD) of IOP, and IOP max indicates the maximum value of IOP during the follow-up. The relationship between these parameters was analyzed with a linear mixed-effects model, adjusting for clinical parameters including age, gender, and axial length. RESULTS: IOP increased after DRPT with a mean of 6.13 ± 3.55 mmHg. IOP max was significantly associated with IOP after DRPT (β = 0.38; p < 0.001). IOP fluctuation was significantly associated with IOP change in DRPT (β = 0.29; p = 0.007). CONCLUSION: Our findings suggest that short-term and relatively long-term IOP dynamics are associated. Long-term IOP dynamics can be predicted by DRPT to some extent.
Factors associated with intrachoroidal cavitation and sinkhole formation in eyes with glaucomatous visual-field defects. International-journal

Chiaki Yamaguchi, Naoki Kiyota, Naoki Takahashi, Yoko Takeda, Kazuko Omodaka, Satoru Tsuda, Toru Nakazawa

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie　2023/10/04

DOI： 10.1007/s00417-023-06247-2 　

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PURPOSE: To investigate factors associated with intrachoroidal cavitation (ICC) and sinkhole formation in eyes with glaucomatous visual-field defects. METHODS: This retrospective, cross-sectional study enrolled a total of 2808 eyes of 1482 patients who were diagnosed/treated for glaucoma and underwent swept-source optical coherence tomography (OCT). We first determined the prevalence of ICCs and sinkholes and their locations. Next, we selected one eye from each patient and compared the clinical characteristics of eyes with and without ICCs. Finally, in eyes with ICCs, we compared the clinical characteristics of eyes with and without sinkholes. Blood flow (BF), represented by laser speckle flowgraphy-measured tissue-area mean blur rate (MBR), was measured in the temporal optic nerve head (ONH), temporal peripapillary chorioretinal atrophy (PPA) zone, and in the ICC zone. ICC area and angle were analyzed in OCT en-face images. Mean deviation and total deviation in the central area (TD-central) were measured with Humphrey visual-field testing. RESULTS: A total of 86 eyes (3.1%) had ICCs and 52 eyes (1.9%) had sinkholes. ICC eyes had a lower spherical equivalent and longer axial length (AL) than non-ICC eyes (P < 0.05). Patients with eyes with sinkholes were more elderly and had worse best-corrected visual acuity, worse TD-central, a larger ICC, and lower tissue-area MBR in the temporal ONH, temporal PPA zone, and ICC zone (P < 0.05). CONCLUSION: In eyes with glaucoma, AL elongation might be linked to ICC formation. Sinkhole formation might be associated with ICC enlargement, impaired ocular BF, and impaired retinal structure and function involving the central area.
Predictive potential of optical coherence tomography parameters for the prognosis of decreased visual acuity after trabeculectomy in open-angle glaucoma patients with good vision. International-journal

Yoko Takeda, Naoki Takahashi, Naoki Kiyota, Taiki Kokubun, Satoru Tsuda, Kazuko Omodaka, Yu Yokoyama, Toru Nakazawa

BMC ophthalmology　23　(1)　399-399　2023/10/04

DOI： 10.1186/s12886-023-03145-3 　

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BACKGROUND: Trabeculectomy (trab) is the most effective surgical procedure for lowering IOP and preventing glaucoma progression. However, decline in best-corrected visual acuity (BCVA) is one of the most serious postoperative complications of trab. Here, we investigated methods to predict decreased BCVA after trab in glaucoma patients with good preoperative BCVA. METHODS: This study included 35 eyes of 35 open-angle glaucoma (OAG) patients (male / female: 21 / 14, age: 64.0 ± 9.7 years old, preoperative intraocular pressure: 15.9 ± 5.4 mmHg, mean deviation: -18.1 ± 5.6 dB) with preoperative BCVA of 0.7 or better who underwent trab and were observed for more than 12 months. As a preoperative analysis, we measured temporal quadrant circumpapillary retinal nerve fiber layer thickness (cpRNFLT) and ganglion cell complex thickness in a central strip between the disc and fovea (csGCCT), an area that corresponds to the location of the papillomacular bundle (PMB) in swept-source optical coherence tomography (OCT). We defined BCVA decline as a loss of more than 3 lines of BCVA after 12 months. Measurement parameters were compared between the BCVA-decline group and the non-BCVA-decline group. RESULTS: BCVA decline was detected in 11 cases (31.4%) 12 months after trab. There was a statistically significant difference in axial length (P = 0.049). A single logistic analysis showed that the BCVA-decline group had significantly lower cpRNFLT than the non-BCVA-decline group (27.7 ± 8.0 μm vs. 45.1 ± 5.3 μm, P < 0.001, cut-off value: 33.4 μm), as well as lower csGCCT (72.4 ± 7.7 μm vs. 87.5 ± 5.1 μm, P = 0.002, cut-off value: 82.3 μm). Multivariable logistic analysis showed that the BCVA-decline group had significantly lower temporal quadrant cpRNFLT (P < 0.001) and lower middle csGCCT (P < 0.001) compared to the non-BCVA-decline group. CONCLUSIONS: Lower temporal quadrant cpRNFLT and middle csGCCT, OCT scan areas that correspond to the location of the PMB, might be biomarkers that predict BCVA decline after trab in OAG patients with good vision.
家庭における血圧や脈拍と緑内障との関連について

前川重人, 津田聡, 石川誠, 中澤徹

日本緑内障学会抄録集　34回　157-157　2023/09
Publisher: 日本緑内障学会
緑内障眼における暗所うつむき負荷試験前後の眼圧と視野障害進行との関連性における視野セクター別の差異

佐藤正隆, 清田直樹, 矢花武史, 前川重人, 津田聡, 面高宗子, 檜森紀子, 横山悠, 中澤徹

日本緑内障学会抄録集　34回　148-148　2023/09
Publisher: 日本緑内障学会
偏光OCTを用いた濾過胞瘢痕化自動評価システムの確立

村上真之, 津田聡, 吉田真彰, 國分太貴, 山成正宗, 岡本道明, 横山悠, 矢花武史, 前川重人, 檜森紀子, 中澤徹

日本緑内障学会抄録集　34回　155-155　2023/09
Publisher: 日本緑内障学会
緑内障患者における機械学習を用いた睡眠時無呼吸症候群の予測

山崎舞, 檜森紀子, 二宮高洋, 清田直樹, 矢花武史, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本緑内障学会抄録集　34回　197-197　2023/09
Publisher: 日本緑内障学会
緑内障眼における暗所うつむき負荷試験前後の眼圧と視野障害進行との関連性における視野セクター別の差異

佐藤正隆, 清田直樹, 矢花武史, 前川重人, 津田聡, 面高宗子, 檜森紀子, 横山悠, 中澤徹

日本緑内障学会抄録集　34回　148-148　2023/09
Publisher: 日本緑内障学会
偏光OCTを用いた濾過胞瘢痕化自動評価システムの確立

村上真之, 津田聡, 吉田真彰, 國分太貴, 山成正宗, 岡本道明, 横山悠, 矢花武史, 前川重人, 檜森紀子, 中澤徹

日本緑内障学会抄録集　34回　155-155　2023/09
Publisher: 日本緑内障学会
家庭における血圧や脈拍と緑内障との関連について

前川重人, 津田聡, 石川誠, 中澤徹

日本緑内障学会抄録集　34回　157-157　2023/09
Publisher: 日本緑内障学会
緑内障患者における機械学習を用いた睡眠時無呼吸症候群の予測

山崎舞, 檜森紀子, 二宮高洋, 清田直樹, 矢花武史, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本緑内障学会抄録集　34回　197-197　2023/09
Publisher: 日本緑内障学会
Glutathione trisulfide prevents lipopolysaccharide-induced retinal inflammation via inhibition of proinflammatory cytokine production in glial cells. International-journal

Hiroshi Tawarayama, Kota Umeki, Maki Inoue-Yanagimachi, Naoki Takahashi, Hirokazu Hasegawa, Noriko Himori, Satoru Tsuda, Hiroshi Kunikata, Takaaki Akaike, Toru Nakazawa

Scientific reports　13　(1)　11513-11513　2023/07/17

DOI： 10.1038/s41598-023-38696-4 　

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We aimed to investigate the impact of glutathione trisulfide (GSSSG) on lipopolysaccharide (LPS)-induced inflammation in retinal glia. Inflammatory responses in mouse-derived glial cells and Wistar rat retinas were stimulated with administration of LPS. Cell survival and proinflammatory cytokine production were examined using the Calcein-AM assay, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Retinal microglia were visualized with immunohistochemistry for Iba1. Administration of LPS (10 µg/mL) or GSSSG (less than 100 µM) did not affect survival of cultured primary Müller cells and established microglial cells (BV-2). RT-qPCR and ELISA indicated that GSSSG inhibited LPS-induced gene upregulation and protein secretion of proinflammatory cytokines in these glial cells and rat retinas. GSSSG inhibited LPS-induced activation of TGF-β-activated kinase 1 (TAK1), which is an upstream kinase of NF-κB, in BV-2 cells. Finally, in vivo experiments indicated that intravitreal administration of GSSSG but not its relative glutathione disulfide (GSSG) inhibited LPS (500 ng)-induced accumulation of Iba1-immunopositive microglia in rat retinas. Taken together, GSSSG has the potential to prevent pathogenesis of inflammation-associated ocular diseases by inhibiting proinflammatory cytokine expression in retinal glial cells.
Factors Associated With Visual Acuity Decline in Glaucoma Patients With Loss of Ganglion Cell Complex Thickness. International-journal

Naoki Takahashi, Kazuko Omodaka, Tsutomu Kikawa, Takahiro Ninomiya, Naoki Kiyota, Satoru Tsuda, Noriko Himori, Toru Nakazawa

Translational vision science & technology　12　(7)　2-2　2023/07/03

DOI： 10.1167/tvst.12.7.2 　

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PURPOSE: To investigate the association between ocular/systemic factors and visual acuity decline in glaucoma patients with loss of ganglion cell complex thickness (GCCT). METHODS: In 515 eyes of 515 patients with open-angle glaucoma (mean age, 62.6 ± 12.8 years; mean deviation, -10.95 ± 9.07 dB), we used swept-source optical coherence tomography to measure macular GCCT in sectors classified as corresponding to circumpapillary retinal nerve fiber layer clock-hour sectors from 7 o'clock (inferotemporal) to 11 o'clock (superotemporal). We calculated Spearman's rank correlation coefficient between each sector and best-corrected visual acuity (BCVA), determined cutoff values for BCVA decline (<20/25), and used multivariable linear regression models to determine the correlation between BCVA and biological antioxidant potential (BAP), corneal hysteresis (CH), and temporal-tissue optic nerve head blood flow (represented by temporal mean blur rate, or MBR-T). RESULTS: Macular GCCT corresponding to the 9 o'clock sector had the highest correlation with BCVA (Rs = -0.454; P < 0.001) and a cutoff of 76.17 µm (area under the receiver operating characteristic curve = 0.891; P < 0.001). Subjects below this cutoff (N = 173) showed significant correlations between BCVA and age, BAP, CH, and MBR-T (β = 0.192, P = 0.033; β = -0.186, P = 0.028; β = -0.217, P = 0.011; and β = -0.222, P = 0.010, respectively). CONCLUSIONS: Multiple factors are involved in BCVA decline in patients with glaucoma with decreased macular GCCT. This suggests that evaluating BCVA may require assessing multiple factors. TRANSLATIONAL RELEVANCE: Multiple factors contribute to BCVA decline.
DPP-4 Inhibitors Attenuate Fibrosis After Glaucoma Filtering Surgery by Suppressing the TGF-β/Smad Signaling Pathway. International-journal

Masaaki Yoshida, Taiki Kokubun, Kota Sato, Satoru Tsuda, Yu Yokoyama, Noriko Himori, Toru Nakazawa

Investigative ophthalmology & visual science　64　(10)　2-2　2023/07/03

DOI： 10.1167/iovs.64.10.2 　

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PURPOSE: This study investigated the effect of dipeptidyl peptidase-4 inhibitors (DPP-4is) on fibrosis after glaucoma filtering surgery with clinical data and an in vitro model that used transforming growth factor-β (TGF-β) to induce human Tenon's fibroblast (HTF) fibrosis. METHODS: The medical records of 41 eyes of 35 patients with diabetes with neovascular glaucoma (NVG) who received initial trabeculectomy were retrospectively reviewed. The surgical success rate was compared between cases that received (n = 23) and did not receive (n = 18) DPP-4i treatment for diabetes. The antifibrotic effects of linagliptin (a DPP-4i) were evaluated with quantitative real-time PCR for fibrosis markers (α-smooth muscle actin, collagen Iα, and fibronectin), a scratch assay, and a collagen gel contraction assay of primary cultured HTFs treated with TGF-β1 and linagliptin. Western blotting analysis was performed to evaluate the levels of phosphorylated Smad2 and Smad3 in the presence of linagliptin. RESULTS: The Kaplan-Meier curve for bleb survival was higher in patients who received DPP-4is (P = 0.017, log-rank test). The in vitro experiments demonstrated that treatment with linagliptin attenuated the elevated levels of fibrosis markers induced by TGF-β1 in HTFs. Linagliptin treatment also prevented the migration and gel contraction of HTFs. Linagliptin inhibited the phosphorylation of Smad2 and Smad3, which is the canonical pathway of TGF-β signaling. CONCLUSIONS: The current study indicates the potential effect of DPP-4is for maintaining bleb function after glaucoma filtering surgery in patients with diabetes with NVG. Our results demonstrate that linagliptin attenuates fibrotic change in HTFs by inhibiting TGF-β/Smad signaling.
Reduced Plasma BDNF Levels in Normal Tension Glaucoma Compared to Open Angle Glaucoma. International-journal

Kota Sato, Naoko Takada, Amane Fujioka, Noriko Himori, Yu Yokoyama, Satoru Tsuda, Kazuko Omodaka, Tomoko Kirihara, Makoto Ishikawa, Hiroshi Kunikata, Toru Nakazawa

Journal of glaucoma　2023/06/19

DOI： 10.1097/IJG.0000000000002257 　

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AIMS: To evaluate differences in systemic BDNF levels between primary open-angle glaucoma (POAG) patients and normal-tension glaucoma (NTG) patients. METHODS: This study collected blood samples from 260 NTG patients, 220 age-matched POAG patients, and 120 age-matched cataract patients (as controls). BDNF levels were measured with an antibody-conjugated bead assay system (Luminex). RESULTS: We found that plasma BDNF levels in the NTG group were significantly lower than in the POAG and cataract control groups. There was no significant difference between the POAG and cataract groups. CONCLUSION: This result suggests that a low level of systemic BDNF may contribute to the pathogenesis of glaucoma in an IOP-independent manner.
Long-Term Surgical Outcomes and Possible Postoperative Complication with Severe Corneal Endothelial Cell Loss After Trabeculectomy for Cytomegalovirus-Associated Anterior Uveitis with Secondary Glaucoma. International-journal

Masaaki Yoshida, Yu Yokoyama, Taiki Kokubun, Satoru Tsuda, Noriko Himori, Shigeto Maekawa, Shunji Yokokura, Takehiro Hariya, Wataru Kobayashi, Kazuki Hashimoto, Toru Nakazawa

Ocular immunology and inflammation　1-9　2023/04/24

DOI： 10.1080/09273948.2023.2197497 　

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PURPOSE: We assess long-term surgical outcomes after an initial trabeculectomy for cytomegalovirus-associated anterior uveitis with secondary glaucoma (CMV-SG). METHODS: We retrospectively reviewed the medical records of 16 eyes of 15 patients with CMV-SG and 157 eyes of 157 patients with primary open-angle glaucoma. The average follow-up period was approximately 3 years. Surgical success was defined as intraocular pressure (IOP) below 18 mmHg and at least 20% lower than baseline. RESULTS: Kaplan-Meier survival analysis revealed that bleb survival rates were not significantly different in the CMV-SG and POAG groups (P = 0.75). Bullous keratopathy occurred in 2 of 16 eyes with CMV-SG postoperatively but did not occur in the POAG group. The corneal endothelial cell density decreased by 34.2 ± 22.7% in the CMV-SG group during an average follow-up period of 2.7 ± 2.0 years. CONCLUSION: Trabeculectomy effectively controlled IOP in CMV-SG, but attention must be paid to corneal endothelial cell loss.
腹臥位負荷前後の眼圧と、経過中眼圧との関連性について

佐藤正隆, 清田直樹, 矢花武史, 前川重人, 津田聡, 面高宗子, 檜森紀子, 横山悠, 中澤徹

日本眼科学会雑誌　127　(臨増)　188-188　2023/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
上眼瞼を介して測定する眼圧計の使用感と安全性における評価

山口万里奈, 津田聡, 山口明彦, 矢花武史, 山口知暁, 根本靖久, 中澤徹

日本眼科学会雑誌　127　(臨増)　189-189　2023/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
緑内障眼における傍乳頭脈絡膜微小血管脱落面積の計測方法に関する検討

高橋直樹, 面高宗子, 木川勉, 二宮高洋, 清田直樹, 津田聡, 中澤徹

日本眼科学会雑誌　127　(臨増)　190-190　2023/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
脈絡膜内洞様構造を伴う眼におけるsinkhole形成に関連する因子について

山口知暁, 清田直樹, 高橋直樹, 武田瑶子, 佐藤正隆, 津田聡, 面高宗子, 中澤徹

日本眼科学会雑誌　127　(臨増)　190-190　2023/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
正常眼圧緑内障患者における睡眠時無呼吸症候群のリスク因子の検討

山崎舞, 檜森紀子, 宮武博, 二宮高洋, 矢花武史, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本眼科学会雑誌　127　(臨増)　220-220　2023/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
新規治療開発への利活用を見据えた緑内障レジストリ

阿部真以子, 津田聡, 和田信介, 横山悠, 檜森紀子, 矢花武史, 面高宗子, 前川重人, 高田菜生子, 山崎舞, 漆原尚巳, 中澤徹

日本眼科学会雑誌　127　(臨増)　221-221　2023/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
A quality management system aiming to ensure regulatory-grade data quality in a glaucoma registry. International-journal

Shinsuke Wada, Satoru Tsuda, Maiko Abe, Toru Nakazawa, Hisashi Urushihara

PloS one　18　(6)　e0286669　2023

DOI： 10.1371/journal.pone.0286669 　

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BACKGROUND: Disease/patient registries are underutilized despite their multiple advantages over clinical trials in the clinical evaluation of drugs, such as the capacity for long-term curation, provision of patient outcome data in routine clinical practice, and provision of benchmark data for comparison. Ensuring the fit-for-purpose quality of data generated from such registries is important to informing regulatory decision making. Here, we report the construction of a quality management system aiming to ensure regulatory-grade data quality for a registry of Japanese patients with glaucoma to evaluate long-term patient outcomes. METHODS: The quality management system was established by reference to the risk-based approach in the ICH-E6 (R2) recommendations. The following three-component approach was taken: establishment of governance, computerized system validation (CSV), and implementation of risk assessment and control. Compliance of the system with the recommendations of regulatory guidelines relevant to use of the registry was assessed. RESULTS: Governance by academic collaboration was established. This was followed by the development of a total of 15 standard operating procedures, including CSV, data management, monitoring, audit, and management of imaging data. The data management system was constructed based on a data management plan, which specified data/paper flow and data management procedures. The electronic data capture (EDC) system was audited by an external vendor, and configured and validated using the V-model framework as recommended in the GAMP5 guideline. Informed consent, eligibility assessment and major ophthalmology measurements were determined as Critical to Quality (CTQ) factors. A total of 22 risk items were identified and classified into three categories, and operationalized in the form of a risk control plan, which included training sessions and risk-based monitoring. The glaucoma registry addressed most quality recommendations in official guidelines issued by multiple health authorities, although two recommendations were not met. CONCLUSIONS: We established and configured a quality management system for a glaucoma registry to ensure fit-for-purpose data quality for regulatory use, and to curate long-term follow-up data of glaucoma patients in a prospective manner.
薬事利用を目指した緑内障レジストリでの品質管理システムの設計および実装

和田信介, 津田聡, 阿部真以子, 中澤徹, 漆原尚巳

日本臨床薬理学会学術総会抄録集　43回　1-7　2022/12
Publisher: (一社)日本臨床薬理学会
eISSN： 2436-5580
Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides. International-journal

Hiroshi Kunikata, Hiroshi Tawarayama, Satoru Tsuda, Takaaki Akaike, Toru Nakazawa

Scientific reports　12　(1)　19243-19243　2022/11/10

DOI： 10.1038/s41598-022-21677-4 　

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Anti-oxidative intraocular irrigating solutions (IISs) based on reactive persulfides, such as oxidized glutathione disulfide (GSSG), are commonly used worldwide. However, even with GSSG-based IISs, it has been shown that oxidative stress can occur during surgery, posing a risk to intraocular tissues. This study compared two IISs: one containing GSSG and one containing an oxidized glutathione trisulfide (GSSSG). Experimental in vivo irrigation with the IISs in rabbits showed that there was less leakage into the anterior chamber of rabbit serum albumin during perfusion with a 300-μM GSSSG IIS than with a 300-μM GSSG IIS. Experimental in vivo cataract surgery in rabbits showed that aqueous flare was suppressed 3 days after surgery with a 600-μM GSSSG IIS, but not with a 300-μM GSSSG or 300-μM GSSG IIS. Furthermore, an in vitro experiment, without any live tissue, showed that reactive oxygen species were suppressed more strongly with a 600-μM GSSSG IIS than with a 300-μM GSSG IIS. Thus, this study found that novel IISs based on GSSSG had anti-inflammatory and anti-oxidative effects during and after intraocular surgery and may decrease the rate of complications after surgery.
Deep Learning-Based Noise Reduction Improves Optical Coherence Tomography Angiography Imaging of Radial Peripapillary Capillaries in Advanced Glaucoma. International-journal

Kazuko Omodaka, Juun Horie, Hikari Tokairin, Chiho Kato, Junko Ouchi, Takahiro Ninomiya, Sharma Parmanand, Satoru Tsuda, Toru Nakazawa

Current eye research　47　(12)　1-9　2022/09/22

DOI： 10.1080/02713683.2022.2124275 　

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PURPOSE: We applied deep learning-based noise reduction (NR) to optical coherence tomography-angiography (OCTA) images of the radial peripapillary capillaries (RPCs) in eyes with glaucoma and investigated the usefulness of this method as an objective analysis of glaucoma. METHODS: This cross-sectional study included 118 eyes of 94 open-angle glaucoma patients (male/female = 38/56, age: 56.1 ± 10.3 years). We used OCTA (OCT-HS100, Canon) and built-in software (RX software, v. 4.5) to perform NR and calculate RPC vessel area density (VAD) and skeleton vessel length density (VLD). We also examined NR's effect on reproducibility. Finally, we assessed the vascular structure (PRCs)/function relationship at different glaucoma stages with Spearman's correlation. RESULTS: Regardless of NR, RPC parameters had excellent coefficients of variation (1.7-4.1%) in glaucoma patients and controls, and mean deviation (MD) was significantly correlated with VAD (NR: r = 0.835, p < 0.001; non-NR: r = 0.871, p < 0.001) and VLD (NR: r = 0.829, p < 0.001; non-NR: r = 0.837, p < 0.001). For mild, moderate, and advanced glaucoma, the correlation coefficients between MD and VLD were 0.366 (p = 0.028) 0.081 (p = 0.689), and 0.427 (p = 0.017) with NR and 0.405 (p = 0.014), 0.184 (p = 0.360), and 0.339 (p = 0.062) without NR, respectively. CONCLUSION: Denoised RPC images might have the potential for a closer structural/functional relationship, in which the floor effect of retinal nerve fiber layer thickness affects measurements. Deep learning-based NR promises to improve glaucoma assessment.
Clinical characteristics of glaucoma patients with various risk factors. International-journal

Kazuko Omodaka, Tsutomu Kikawa, Sayaka Kabakura, Noriko Himori, Satoru Tsuda, Takahiro Ninomiya, Naoki Takahashi, Kyongsun Pak, Noriyasu Takeda, Masahiro Akiba, Toru Nakazawa

BMC ophthalmology　22　(1)　373-373　2022/09/19

DOI： 10.1186/s12886-022-02587-5 　

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BACKGROUND: Glaucoma is multifactorial, but the interrelationship between risk factors and structural changes remains unclear. Here, we adjusted for confounding factors in glaucoma patients with differing risk factors, and compared differences in structure and susceptible areas in the optic disc and macula. METHODS: In 458 eyes with glaucoma, we determined confounding factors for intraocular pressure (IOP), central corneal thickness (CCT), axial length (AL), LSFG-measured ocular blood flow (OBF), which was assessed with laser speckle flowgraphy-measured mean blur rate in the tissue area (MT) of the optic nerve head, biological antioxidant potential (BAP), and systemic abnormalities in diastolic blood pressure (dBP). To compensate for measurement bias, we also analyzed corrected IOP (cIOP; corrected for CCT) and corrected MT (cMT; corrected for age, weighted retinal ganglion cell count, and AL). Then, we determined the distribution of these parameters in low-, middle-, and high-value subgroups and compared them with the Kruskal-Wallis test. Pairwise comparisons used the Steel-Dwass test. RESULTS: The high-cIOP subgroup had significantly worse mean deviation (MD), temporal, superior, and inferior loss of circumpapillary retinal nerve fiber layer thickness (cpRNFLT), and large cupping. The low-CCT subgroup had temporal cpRNFLT loss; the high-CCT subgroup had low cup volume. The high-AL subgroup had macular ganglion cell complex thickness (GCCT) loss; the low-AL subgroup had temporal cpRNFLT loss. The high-systemic-dBP subgroup had worse MD, total, superior, and inferior cpRNFLT loss and macular GCCT loss. The low-BAP subgroup had more male patients, higher dBP, and cpRNFLT loss in the 10 o'clock area. The high-OBF subgroup had higher total, superior and temporal cpRNFLT and macular GCCT. CONCLUSIONS: Structural changes and local susceptibility to glaucomatous damage show unique variations in patients with different risk factors, which might suggest that specific risk factors induce specific types of pathogenesis and corresponding glaucoma phenotypes. Our study may open new avenues for the development of precision medicine for glaucoma.
開放隅角緑内障における水晶体再建術併用維線柱帯切除術後の視力予後予測因子の検討

武田瑶子, 高橋直樹, 國分太貴, 山口知暁, 津田聡, 面高宗子, 中澤徹

日本緑内障学会抄録集　33回　87-87　2022/09
Publisher: 日本緑内障学会
偏光OCTの濾過胞瘢痕化の自動解析のための自動眼瞼除去プログラム

津田聡, 國分太貴, 佐藤正隆, 清田直樹, 横山悠, 山成正宏, 岡本道明, 中澤徹

日本緑内障学会抄録集　33回　87-87　2022/09
Publisher: 日本緑内障学会
エクオール産生能と緑内障との検討

内田惠子, 檜森紀子, 永井雅, 二宮高洋, 矢花武史, 前川重人, 津田聡, 面高宗子, 横山悠, 佐藤孝太, 中澤徹

日本緑内障学会抄録集　33回　90-90　2022/09
Publisher: 日本緑内障学会
脈絡膜内洞様構造形成に寄与する因子について

山口知暁, 中澤徹, 面高宗子, 津田聡, 高橋直樹, 清田直樹, 武田遥子

日本緑内障学会抄録集　33回　91-91　2022/09
Publisher: 日本緑内障学会
緑内障点眼アドオン前後における血圧および脈拍の変化について

清田直樹, 志賀由己浩, 二宮高洋, 宮武博, 津田聡, 面高宗子, 檜森紀子, 横山悠, 中澤徹

日本緑内障学会抄録集　33回　93-93　2022/09
Publisher: 日本緑内障学会
乳頭黄斑神経線維が菲薄化した緑内障眼における視力低下と関連する因子の検討

高橋直樹, 面高宗子, 木川勉, 武田瑶子, 山口知暁, 清田直樹, 津田聡, 中澤徹

日本緑内障学会抄録集　33回　142-142　2022/09
Publisher: 日本緑内障学会
プラトー虹彩を有する閉塞隅角症例の眼軸の検討

佐久間大輔, 矢花武史, 佐藤正隆, 清田直樹, 津田聡, 横山悠, 中澤徹

日本緑内障学会抄録集　33回　142-142　2022/09
Publisher: 日本緑内障学会
CADi2を用いて測定した認知機能と緑内障性視野障害重症度の関連性

佐藤正隆, 山田晃生, 清田直樹, 津田聡, 大木広美, 吉田光秀, 冨田尚希, 國方彦志, 瀧靖之, 中澤徹

日本緑内障学会抄録集　33回　88-88　2022/09
Publisher: 日本緑内障学会
カニクイザルを用いたHMRG硝子体内投与時の蛍光励起波長光による網膜光毒性試験

稲岡志織, 荒木智陽, 坂田恵美, 樺山浩二, 友廣雅之, 國井峻, 鈴木悠記, 津田聡, 中澤徹

比較眼科学会年次大会講演要旨集　41回　35-35　2022/07
Publisher: 比較眼科学会
Correlation Between Enlargement of Retinal Nerve Fiber Defect Angle in En Face Imaging and Visual Field Progression. International-journal

Naoki Takahashi, Kazuko Omodaka, Arata Nakazawa, Tsutomu Kikawa, Takahiro Ninomiya, Naoki Kiyota, Satoru Tsuda, Noriko Himori, Masahiro Akiba, Toru Nakazawa

Translational vision science & technology　11　(6)　8-8　2022/06/01

DOI： 10.1167/tvst.11.6.8 　

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Purpose: Retinal nerve fiber layer defects (RNFLDs) become enlarged with glaucoma progression. We measured the RNFLD angle and investigated whether it was correlated with deterioration of the visual field in patients with glaucoma. Methods: This study included 84 eyes of 84 patients with open-angle glaucoma (mean deviation [MD] = -6.51 ± 5.91 dB, follow-up period = 2.82 ± 0.74 years) with the RNFLDs, who underwent en face swept-source optical coherence tomography (SS-OCT) wide scans (12 × 9 mm) at least 6 times. The RNFLD angle was measured as the intersection between the RNFLD and a circle centered on the disc with a radius half the distance between the disc and the fovea. Slopes for the RNFLD angle, macular ganglion cell layer thickness (GCCT), and circumpapillary RNFL thickness (cpRNFLT) were compared with the MD slope, as measured with the Humphrey field analyzer 24-2 program. Results: The correlation coefficients with MD slope were -0.67 for the RNFLD angle slope (P < 0.001), 0.15 for the macular GCCT slope (P = 0.163), and 0.04 for the cpRNFLT slope (P = 0.719). The RNFLD angle tended to increase as the number of disc hemorrhage occurrences increased (rs = 0.31, P = 0.004). The RNFLD angle slope also had good predictive power for glaucoma progression (area under the receiver operating characteristic curve = 0.88, 95% confidence interval = 0.81-0.95). Conclusions: We found that the RNFLD angle slope was more closely associated with the MD slope than were other OCT parameters. This suggests that measurement of the RNFLD angle with en face OCT images could be effective in evaluating glaucoma progression. Translational Relevance: Our study provides a method for monitoring glaucoma progression with SS-OCT.
正常眼圧緑内障患者における睡眠時無呼吸症候群の男女別リスク因子の検討

山崎舞, 檜森紀子, 矢花武史, 國分太貴, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本眼科学会雑誌　126　(臨増)　271-271　2022/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
AP7000を用いた10点選択検査法の前向き研究における中間報告

矢花武史, 津田聡, 若梅紗織, 杉山真生, 高津育美, 島田賢, 横山悠, 中澤徹

日本眼科学会雑誌　126　(臨増)　172-172　2022/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
マイクロパルス波経強膜光凝固術のレーザーファイバー形状の影響

津田聡, 國分太貴, 桐山卓也, 岡部達, 山成正宏, 岡本道明, 中澤徹

日本眼科学会雑誌　126　(臨増)　242-242　2022/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
乳頭黄斑線維束に着目した緑内障における線維柱帯切除術後の視力低下

武田瑶子, 高橋直樹, 國分太貴, 津田聡, 面高宗子, 中澤徹

日本眼科学会雑誌　126　(臨増)　253-253　2022/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
正常眼圧緑内障患者における睡眠時無呼吸症候群の男女別リスク因子の検討

山崎舞, 檜森紀子, 矢花武史, 國分太貴, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本眼科学会雑誌　126　(臨増)　271-271　2022/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
【網膜循環疾患アップデート】網膜動脈閉塞症

津田聡, 中澤徹

あたらしい眼科　39　(1)　31-37　2022/01
Publisher: (株)メディカル葵出版
ISSN： 0910-1810
正常眼圧緑内障における血中エンドセリン濃度とFlammer症候群および視野進行の関連

高橋成奈, 佐藤孝太, 高田菜生子, 志賀由己浩, 清田直樹, 安田正幸, 津田聡, 面高宗子, 中澤徹

日本緑内障学会抄録集　32回　80-80　2021/09
Publisher: 日本緑内障学会
マイクロパルス波経強膜光凝固術の新型プローブ(Rev2)の照射条件の検討

津田聡, 國分太貴, 桐山卓也, 岡部達, 山成正宏, 岡本道明, 中澤徹

日本緑内障学会抄録集　32回　131-131　2021/09
Publisher: 日本緑内障学会
正常眼圧緑内障患者における睡眠時無呼吸症候群の有病率とリスク因子の検討

山崎舞, 檜森紀子, 矢花武史, 國分太貴, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本緑内障学会抄録集　32回　147-147　2021/09
Publisher: 日本緑内障学会
当院におけるマイクロフックロトミー後の前房出血に対する前房洗浄の短期予後

矢花武史, 國分太貴, 前川重人, 津田聡, 横山悠, 中澤徹

日本緑内障学会抄録集　32回　166-166　2021/09
Publisher: 日本緑内障学会
正常眼圧緑内障における血中エンドセリン濃度とFlammer症候群および視野進行の関連

高橋成奈, 佐藤孝太, 高田菜生子, 志賀由己浩, 清田直樹, 安田正幸, 津田聡, 面高宗子, 中澤徹

日本緑内障学会抄録集　32回　80-80　2021/09
Publisher: 日本緑内障学会
マイクロパルス波経強膜光凝固術の新型プローブ(Rev2)の照射条件の検討

津田聡, 國分太貴, 桐山卓也, 岡部達, 山成正宏, 岡本道明, 中澤徹

日本緑内障学会抄録集　32回　131-131　2021/09
Publisher: 日本緑内障学会
正常眼圧緑内障患者における睡眠時無呼吸症候群の有病率とリスク因子の検討

山崎舞, 檜森紀子, 矢花武史, 國分太貴, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本緑内障学会抄録集　32回　147-147　2021/09
Publisher: 日本緑内障学会
当院におけるマイクロフックロトミー後の前房出血に対する前房洗浄の短期予後

矢花武史, 國分太貴, 前川重人, 津田聡, 横山悠, 中澤徹

日本緑内障学会抄録集　32回　166-166　2021/09
Publisher: 日本緑内障学会
Usefulness of Polarization-sensitive Optical Coherence Tomography-derived Attenuation-coefficient Images to Visualize the Internal Structure of the Filtering Bleb. International-journal

Mayo Kosugi, Taiki Kokubun, Satoru Tsuda, Masahiro Yamanari, Toru Nakazawa

Current eye research　46　(4)　606-609　2021/04

DOI： 10.1080/02713683.2020.1825749 　

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PURPOSE/AIM OF THE STUDY: Polarization-sensitive anterior-segmental optical coherence tomography (PS-OCT) is useful to evaluate the filtering blebs after trabeculectomy. However, the attenuation phenomenon of OCT signal disturbs the visibility of intra-bleb's structure and distribution of birefringence; a specific parameter of PS-OCT. This study aimed to evaluate the effectiveness of the attenuation-coefficient image of the filtering blebs. MATERIALS AND METHODS: This study included 25 eyes 6 months after TLE. The attenuation-coefficient image of blebs was calculated from OCT intensity signal information. The contrast level of the image (reflectivity of bleb's surface wall/reflectivity of scleral flap) in both attenuation-coefficient image and intensity image and the height of the bleb's wall were calculated. The improvement extent of contrast was defined as the ratio of contrast (attenuation-coefficient image's contrast/intensity image's contrast). We compared the contrast of attenuation-coefficient image and intensity image and investigated the relationship between improvement extent of contrast and height of bleb's wall. RESULTS: The contrast of the attenuation-coefficient image (317.7 ± 255.4%) was significantly higher than that of the intensity image (39.1 ± 24.5%) (P < .01) and the improvement extent of contrast was 9.5 ± 4.6 times. Furthermore, there was a positive correlation between the improvement extent of contrast and the height of the bleb's wall (r = 0.44, P = .03). CONCLUSIONS: The contrast of the image at depth of filtering blebs was improved by attenuation-coefficient image. It is suggested that the attenuation-coefficient image may improve the visibility of the structure and distribution of birefringence in the blebs compared to the intensity image.
Risk factors leading to trabeculectomy surgery of glaucoma patient using Japanese nationwide administrative claims data: a retrospective non-interventional cohort study. International-journal

Chikako Shirai, Satoru Tsuda, Kunio Tarasawa, Kiyohide Fushimi, Kenji Fujimori, Toru Nakazawa

BMC ophthalmology　21　(1)　153-153　2021/03/29

DOI： 10.1186/s12886-021-01897-4 　

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BACKGROUND: Early recognition and management of baseline risk factors may play an important role in reducing glaucoma surgery burdens. However, no studies have investigated them using real-world data in Japan or other countries. This study aimed to clarify the risk factors leading to trabeculectomy surgery, which is the most common procedure of glaucoma surgery, of glaucoma patient using the Japanese nationwide administrative claims data associated with the diagnosis procedure combination (DPC) system. METHODS: It was a retrospective, non-interventional cohort study. Data were collected from patients who were admitted to DPC participating hospitals, nationwide acute care hospitals and were diagnosed with glaucoma between 2012 to 2018. The primary outcome was the risk factors associated with trabeculectomy surgery. The association between baseline characteristics and trabeculectomy surgery was identified using multivariable logistic regression analysis by comparing patients with and without trabeculectomy surgery. Meanwhile, the secondary outcomes included the rate of comorbidities, the rate of concomitant drug use and the treatment patterns of glaucoma eye drops at the index admission. Among patients with trabeculectomy surgery, the risk factors leading to cataract surgery were also evaluated as subgroup analysis. RESULTS: A total of 29,599 patients included in the analysis, 12,038 and 17,561 patients were in the glaucoma surgery and non-glaucoma surgery cohorts, respectively. The factors associated with the increase in trabeculectomy surgery were having allergies, taking concomitant drugs including cancer, depression, ischemic heart disease and peptic ulcer, being diagnosed with primary open-angle glaucoma and longer length of stay in hospital. In contrast, the factors associated with the decrease in trabeculectomy surgery were having hypertension, taking hypertension drug, age ≥ 80 and female. CONCLUSIONS: Special focus on Japanese patients with glaucoma who have allergy-related comorbidities or take immune, nervous, circulatory or gastrointestinal system-related concomitant drugs seems to be desirable.
家兎でのマイクロパルス波経強膜毛様体光凝固術後の眼圧下降と組織障害の検討

津田聡, 國分太貴, 岡部達, 桐山卓也, 中澤徹

日本眼科学会雑誌　125　(臨増)　175-175　2021/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
正常眼圧白内障手術の角膜内皮保護効果に関する検証

國分太貴, 津田聡, 矢花武史, 前川重人, 横山悠, 國方彦志, 中澤徹

日本眼科学会雑誌　125　(臨増)　188-188　2021/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
正常眼圧緑内障の病期とcorneal hysteresisの関連

内田惠子, 檜森紀子, 國分太貴, 矢花武史, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本眼科学会雑誌　125　(臨増)　240-240　2021/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
The Effect of Dietary Antioxidant Supplementation in Patients with Glaucoma. International-journal

Noriko Himori, Maki Inoue Yanagimachi, Kazuko Omodaka, Yukihiro Shiga, Satoru Tsuda, Hiroshi Kunikata, Toru Nakazawa

Clinical ophthalmology (Auckland, N.Z.)　15　2293-2300　2021

DOI： 10.2147/OPTH.S314288 　

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Purpose: Oxidative stress may be a risk factor for glaucoma, and many previous reports have suggested that antioxidants could be a promising treatment. Here, we investigated the effects of a novel supplement containing three food-derived antioxidants (hesperidin, crocetin, and Tamarindus indica) on markers of oxidative stress in patients with glaucoma. Patients and Methods: This study had a prospective, single arm design. Thirty Japanese glaucoma patients were recruited and given 4 tablets with ample water twice a day for 8 weeks. The treatment was stopped, and the subjects were followed for an additional 8 weeks. We measured biological antioxidant potential (BAP) with a free radical analyzer. We also measured urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG; a marker of oxidative DNA damage). Clinical laboratory data were measured in venous blood samples. Clinical parameters were also recorded. Comparisons used a one-way analysis of variance (ANOVA) followed by Dunnett's test. Results: The 8-OHdG level was not reduced. We also divided the patients into groups with high or low oxidative stress. In patients with relatively high oxidative stress, the 8-OHdG level was significantly reduced at weeks 4, 8, 12, and 16 (P < 0.001, P < 0.01, P < 0.01, P < 0.01), and BAP was significantly elevated at weeks 8 and 12 (P = 0.03, P = 0.04). In patients with relatively low oxidative stress, the 8-OHdG level was not significantly reduced during supplement intake but was significantly elevated at weeks 12 and 16 (P =0.03, P = 0.04), while BAP was not significantly elevated. Conclusion: An 8-week oral course of antioxidant supplementation was effective in patients with a high oxidative stress level. Dietary supplementation could hold promise in the treatment of systemic oxidative stress-related diseases.
Glutathione Trisulfide Prevents Lipopolysaccharide-induced Inflammatory Gene Expression in Retinal Pigment Epithelial Cells. International-journal

Hiroshi Tawarayama, Noriyuki Suzuki, Maki Inoue-Yanagimachi, Noriko Himori, Satoru Tsuda, Kota Sato, Tomoaki Ida, Takaaki Akaike, Hiroshi Kunikata, Toru Nakazawa

Ocular immunology and inflammation　1-12　2020/11/20

DOI： 10.1080/09273948.2020.1833224 　

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We investigated the effects of glutathione trisulfide (GSSSG) on lipopolysaccharide (LPS)-induced inflammatory gene expression in immortalized ARPE-19, and primary human and mouse retinal pigment epithelial (RPE) cells. Sulfane sulfur molecules were significantly increased in GSSSG-treated ARPE-19 cells. GSSSG prevented the LPS-induced upregulation of interleukin (IL)-1β, IL-6, and C-C motif chemokine ligand 2 (CCL2) in ARPE-19/primary RPE cells. Moreover, GSSSG prevented the activation of the nuclear factor-kappa B p65 subunit, and promoted the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) in LPS-treated ARPE-19 cells. ERK1/2 inhibition prevented the GSSSG-mediated inhibition of LPS-induced IL-6 and CCL2 upregulation. Additionally, ERK1/2 activation prevented the upregulation of these genes in the absence of GSSSG. Knockdown of HMOX1 or NRF2, known as anti-oxidative genes, did not affect the activity of GSSSG in the context of LPS stimulation. These findings suggest that GSSSG attenuates LPS-induced inflammatory gene expression via ERK signaling hyperactivation, independently of the NRF2/HMOX1 pathway.
ニードリングの成績と前眼部光干渉断層計による濾過胞構造の関係

小杉真代, 津田聡, 國分太貴, 矢花武史, 前川重人, 横山悠, 中澤徹

日本緑内障学会抄録集　31回　81-81　2020/10
Publisher: 日本緑内障学会
Structural Characterization of Glaucoma Patients with Low Ocular Blood Flow. International-journal Peer-reviewed

Kazuko Omodaka, Shunsuke Fujioka, Guangzhou An, Takuma Udagawa, Satoru Tsuda, Yukihiro Shiga, Soichiro Morishita, Tsutomu Kikawa, Kyongsun Pak, Masahiro Akiba, Hideo Yokota, Toru Nakazawa

Current eye research　45　(10)　1302-1308　2020/10

DOI： 10.1080/02713683.2020.1736306 　

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Purpose: There is an unclear relationship between ocular blood flow (OBF) and the structural characteristics of the optic nerve head (ONH) in glaucoma, a multifactorial disease. This study used laser speckle flowgraphy (LSFG) to identify low-OBF glaucoma patients and investigated the ONH in these patients. Materials and Methods: In 533 eyes with glaucoma, we determined confounding factors for LSFG-measured OBF (tissue-area mean blur rate: MT) and corrected MT with a linear mixed-effects model (LMM). Structural ONH data (from fundus stereo photography), OCT data, and clinical characteristics were then compared in patients with corrected MT in the upper and lower quartiles using the LMM. Results: Single regression showed significant correlations between MT and age, spherical equivalent (SE), central corneal thickness (CCT), and a weighted count of retinal ganglion cells (wRGC), but not axial length or systemic blood pressure. Gender also significantly influenced MT; MT was corrected for these correlated factors and also glaucoma type with the LMM. The lower-quartile MT group had a significantly larger cup area and cup-disc area ratio and lower temporal quadrant circumpapillary retinal nerve fiber layer thickness (cpRNFLT) and macular ganglion cell complex (GCC) than the upper-quartile group. Conclusions: Low-OBF glaucoma patients were characterized by a larger cup-disc ratio, and higher susceptibility to damage in the temporal disc and the macular area.
Companion Diagnosis for Retinal Neuroprotective Treatment by Real-Time Imaging of Calpain Activation Using a Novel Fluorescent Probe. International-journal Peer-reviewed

Toshifumi Asano, Yuri Nagayo, Satoru Tsuda, Azusa Ito, Wataru Kobayashi, Kosuke Fujita, Kota Sato, Koji M Nishiguchi, Hiroshi Kunikata, Hiroyoshi Fujioka, Mako Kamiya, Yasuteru Urano, Toru Nakazawa

Bioconjugate chemistry　31　(9)　2241-2251　2020/09/16

DOI： 10.1021/acs.bioconjchem.0c00435 　

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Calpain activation induces retinal ganglion cell (RGC) death, while calpain inhibition suppresses RGC death, in animal studies. However, the role of calpain in human retinal disease is unclear. This study investigated a new strategy to study the role of calpain based on real-time imaging. We synthesized a novel fluorescent probe for calpain, acetyl-l-leucyl-l-methionine-hydroxymethyl rhodamine green (Ac-LM-HMRG) and used it for real-time imaging of calpain activation. The toxicity of Ac-LM-HMRG was evaluated with a lactate dehydrogenase cytotoxicity assay, retinal sections, and electroretinograms. Here, we performed real-time imaging of calpain activation in a rat model. First, we administered N-methyl-d-aspartate (NMDA) to induce retinal injury. Twenty minutes later, we administered an intravitreal injection of Ac-LM-HMRG. Real-time imaging was then completed with a noninvasive confocal scanning laser ophthalmoscope. The inhibitory effect of SNJ-1945 against calpain activation was also examined with the same real-time imaging method. Ac-LM-HMRG had no toxic effects. The number of Ac-LM-HMRG-positive cells in real-time imaging significantly increased after NMDA injury, and SNJ-1945 significantly lowered the number of Ac-LM-HMRG-positive cells. Real-time imaging with Ac-LM-HMRG was able to quickly quantify the NMDA-induced activation of calpain and the inhibitory effect of SNJ-1945. This technique, used as a companion diagnostic system, may aid research into the development of new neuroprotective therapies.
緑内障患者における複合サプリメント摂取による酸化ストレス、抗酸化力の変動

檜森紀子, 柳町真希, 志賀由己浩, 面高宗子, 津田聡, 國方彦志, 中澤徹

日本抗加齢医学会総会プログラム・抄録集　20回　191-191　2020/09
Publisher: (一社)日本抗加齢医学会
Serum anti-recoverin antibodies is found in elderly patients with retinitis pigmentosa and cancer. International-journal Peer-reviewed

Taimu Sato, Koji M Nishiguchi, Kosuke Fujita, Fuyuki Miya, Takashi Inoue, Erika Sasaki, Toshifumi Asano, Satoru Tsuda, Yukihiro Shiga, Hiroshi Kunikata, Mitsuru Nakazawa, Toru Nakazawa

Acta ophthalmologica　98　(6)　e722-e729　2020/09

DOI： 10.1111/aos.14373 　

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PURPOSE: To screen for anti-recoverin antibodies in elderly patients with retinitis pigmentosa (RP) with or without cancer and cross-sectionally characterize the seropositive patients clinically. METHODS: Serum from 75 RP patients who had been tested for mutations in a panel of 83 RP genes and 73 normal controls, all aged 50-80 years, were screened for anti-recoverin antibodies by Western blot using recombinant recoverin, retinal lysate from a marmoset and commercial anti-recoverin antibodies as a control. RESULTS: Three RP patients with typical pigmentary degeneration of the 75 (4.0%) were seropositive for anti-recoverin antibody. Pathogenic mutations were identified in two seropositive RP patients. All three patients had visual impairment since childhood and were diagnosed as RP by the age of 30. The severity of the retinopathy varied greatly among these three patients, ranging in visual acuity from light perception OU to 20/30 OU. Retinitis pigmentosa (RP) patients with a history of cancer were more likely to have anti-recoverin antibodies (3/14; 21.4%) than those without (0/61; 0%; p = 0.005, Fischer exact test). All 73 healthy controls with no history of cancer were also seronegative. CONCLUSION: Our results show that serum anti-recoverin antibodies can be detected in typical RP patients with identified pathogenic mutations and that a history of cancer may increase the risk of developing anti-recoverin antibodies.
The optic nerve head vasoreactive response to systemic hyperoxia and visual field defect progression in open-angle glaucoma, a pilot study. International-journal Peer-reviewed

Naoki Kiyota, Yukihiro Shiga, Masayuki Yasuda, Naoko Aizawa, Kazuko Omodaka, Satoru Tsuda, Kyongsun Pak, Hiroshi Kunikata, Toru Nakazawa

Acta ophthalmologica　98　(6)　e747-e753　2020/09

DOI： 10.1111/aos.14361 　

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PURPOSE: To investigate the effect of optic nerve head (ONH) tissue vasoreactivity on glaucoma visual field defect progression. METHODS: This prospective, longitudinal study comprised 28 eyes of 16 patients with open-angle glaucoma. Baseline data were obtained from oxygen inhalation testing, including laser speckle flowgraphy measurements of tissue-area mean blur rate (MT), which represents ONH tissue blood flow, and the mean percentage change in MT (MT-change). Follow-up data were collected for at least 2 years, including at least 5 reliable visual field tests. The average total deviation (TD) was calculated in each sector of the Garway-Heath map; in this study, superior, central and inferior TD corresponded to inferior, temporal and superior MT or MT-change, respectively. MT and MT-change in three sectors per eye were included as explanatory variables in a multivariable linear mixed-effects model, with TD slope set as the response variable. RESULTS: At baseline, lower MT and higher diastolic blood pressure were associated with lower MT-change (p < 0.05). Additionally, MT-change significantly contributed to TD slope in the corresponding sectors (β = 0.41, p = 0.01). CONCLUSIONS: Weaker ONH tissue vasoreactivity to systemic hyperoxia, assessed with laser speckle flowgraphy, was associated with lower baseline ONH tissue blood flow, higher diastolic blood pressure and rapid visual field defect progression. Laser speckle flowgraphy variables during oxygen provocation testing may represent potentially useful predictive biomarkers of glaucoma progression.
Progression in Open-Angle Glaucoma with Myopic Disc and Blood Flow in the Optic Nerve Head and Peripapillary Chorioretinal Atrophy Zone

Naoki Kiyota, Yukihiro Shiga, Nana Takahashi, Masayuki Yasuda, Kazuko Omodaka, Satoru Tsuda, Hiroshi Kunikata, Toru Nakazawa

Ophthalmology Glaucoma　3　(3)　202-209　2020/05/01
Publisher: American Academy of Ophthalmology
DOI： 10.1016/j.ogla.2020.03.005 　

ISSN： 2589-4196 2589-4234
A pyruvate dehydrogenase kinase inhibitor prevents retinal cell death and improves energy metabolism in rat retinas after ischemia/reperfusion injury. International-journal Peer-reviewed

Kota Sato, Seiya Mochida, Daisuke Tomimoto, Takahiro Konuma, Naoki Kiyota, Satoru Tsuda, Yukihiro Shiga, Kazuko Omodaka, Toru Nakazawa

Experimental eye research　193　107997-107997　2020/04

DOI： 10.1016/j.exer.2020.107997 　

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We aimed to assess the neuroprotective effect of a pyruvate dehydrogenase kinase (PDK) inhibitor, Nov3r after ischemia/reperfusion (IR) injury in rats. IR injury was induced by applying 150 mmHg of intraocular pressure for 50 min. Nov3r was orally administered (100 mg/kg) 3 h before and 24 h after IR injury. TUNEL-positive cells increased and immunoreactive RBPMS-positive cells decreased in the rat retinas after IR injury. Administration of Nov3r significantly ameliorated the increase in TUNEL-positive cells and prevented the RBPMS-positive cell decrease. Similarly, the number of IR-induced Iba1-positive microglial cells was significantly reduced with Nov3r treatment. Among metabolic parameters, IR damage induced the elevation of lactate and pyruvate, and the reduction of ATP. Oral administration of Nov3r ameliorated these changes. Our data suggest that the Nov3r had a retinal neuroprotective effect in IR injury in rats. This finding suggests that the regulation of pyruvate dehydrogenase (PDH) activity has potential therapeutic value by enabling metabolic reprograming in diseases associated with ischemic retinal damage, such as diabetic retinopathy, retinopathy of prematurity, retinal vein occlusion, ischemic optic neuropathy and glaucoma.
マイクロパルス波経強膜毛様体光凝固術後の6ヵ月間成績

小杉真代, 津田聡, 國分太貴, 前川重人, 横山悠, 中澤徹

日本眼科学会雑誌　124　(臨増)　176-176　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
The association between oxidative stress and corneal hysteresis in patients with glaucoma. International-journal Peer-reviewed

Keiko Uchida, Noriko Himori, Kazuki Hashimoto, Yukihiro Shiga, Satoru Tsuda, Kazuko Omodaka, Toru Nakazawa

Scientific reports　10　(1)　545-545　2020/01/17

DOI： 10.1038/s41598-020-57520-x 　

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Systemic antioxidative status has been implicated in glaucoma pathogenesis. Additionally, corneal hysteresis (CH) may contribute to glaucoma progression. Here, we evaluated the relationship between biological antioxidant potential (BAP) and CH. This study included 103 patients with open-angle glaucoma (OAG). We used a free radical analyzer to measure BAP, and an ocular response analyzer to measure CH and corneal resistance factor (CRF). We evaluated the relationship between systemic oxidative stress and other clinical parameters with Spearman's rank correlation test and a multi-regression analysis. BAP was not correlated to either CH or CRF in the male or female OAG patients. BAP was correlated to both CH and CRF in the female OAG patients older than 57 years (r = 0.51, P = 0.003; r = 0.49, P = 0.004), but uncorrelated in the female OAG patients younger than 57 years. Multiple regression analysis revealed that BAP independently contributed to CH (P = 0.025) and CRF (P = 0.015) in the older female OAG patients. Systemic oxidative stress may significantly affect the viscoelasticity of the cornea in older female OAG patients. Future studies are needed to confirm that low systemic antioxidative status and low corneal hysteresis contribute to glaucoma pathogenesis.
The DNA topoisomerase II inhibitor amsacrine as a novel candidate adjuvant in a model of glaucoma filtration surgery. International-journal Peer-reviewed

Kotaro Yamamoto, Taiki Kokubun, Kota Sato, Takahiro Akaishi, Atsushi Shimazaki, Masatsugu Nakamura, Yukihiro Shiga, Satoru Tsuda, Kazuko Omodaka, Hideyuki Saya, Toru Nakazawa

Scientific reports　9　(1)　19288-19288　2019/12/17

DOI： 10.1038/s41598-019-55365-7 　

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Treatments for refractory glaucoma include trabeculectomy, in which a filtering bleb is created to reduce aqueous pressure. Mitomycin C (MMC) is often used as an adjuvant to reduce post-trabeculectomy bleb scarring and consequent failure. However, scarring sometimes still occurs. Thus, we searched for more effective trabeculectomy adjuvants with high-throughput screening (HTS) of a library of 1,165 off-patent drug compounds. This revealed that amsacrine (AMSA), a DNA topoisomerase II (TOP2) inhibitor, was the top candidate. Compared to MMC, rabbits that underwent trabeculectomy with 10% AMSA had lower IOP at 42, 56, and 70 days (P < 0.01 at all measurement points) and a higher bleb score at 28, 42, 56, and 70 days (P = < 0.01, 0.04, 0.04, and < 0.01, respectively). Compared to saline, rabbits that received 1% AMSA also had lower IOP and better bleb score at all time points, without a sharp drop in IOP just after surgery (all P < 0.01). Both effects were milder than MMC at 7 days (P = 0.02 and <0.01, respectively). Thus, this study showed that HTS may help identify new, promising uses for off-patent drugs. Furthermore, trabeculectomy with AMSA at a suitable concentration may improve the prognosis after trabeculectomy compared to MMC.
Effects of Brimonidine and Timolol on the Progression of Visual Field Defects in Open-angle Glaucoma: A Single-center Randomized Trial. International-journal Peer-reviewed

Yu Yokoyama, Ryo Kawasaki, Hidetoshi Takahashi, Shigeto Maekawa, Satoru Tsuda, Kazuko Omodaka, Toru Nakazawa

Journal of glaucoma　28　(7)　575-583　2019/07

DOI： 10.1097/IJG.0000000000001285 　

ISSN： 1057-0829

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PRéCIS:: Instillation of brimonidine or timolol slowed visual field deterioration in patients with open-angle glaucoma; both brimonidine and timolol might improve the mean deviation (MD) slopes. PURPOSE: The purpose of this study was to investigate and compare the effects of 0.1% brimonidine and 0.5% timolol on the progressing visual field defects in open-angle glaucoma. PATIENTS AND METHODS: We evaluated 1 eye each of 68 glaucoma patients who were treated with at least 1 prostaglandin analog. Their baseline MD slopes were < -0.5 dB/y based on at least 5 Humphrey field analyzer measurements within 3 years. Eligible eyes were randomly assigned to brimonidine or timolol treatment groups and treatments were administered without the wash-out period. Clinical examinations were performed every 4 months for 2 years. We designated the MD slope as the primary endpoint. RESULTS: Ultimately, 56 eyes (brimonidine:timolol=26:30) were included in the present study (mean age=65.2 y). Dropout rates of brimonidine and timolol treatment groups were 27.8% and 6.3%, respectively. There were no significant differences in baseline intraocular pressure or MD slopes between brimonidine and timolol groups (12.7 and 12.9 mm Hg, P=0.77, and -1.22 and -1.08 dB/y, P=0.43, respectively). Intraocular pressure decreased significantly in the brimonidine group at 4, 8, 12, and 16 months, and in the timolol group at 4 months, without significant differences between the drugs (P=0.20). MD slopes significantly improved in both groups (brimonidine: -0.38 dB/y, P<0.001; timolol: -0.52 dB/y, P=0.04). Furthermore, there was no significant difference between groups in the primary endpoint (P=0.59). CONCLUSION: Brimonidine and timolol treatments improved MD slopes in open-angle glaucoma.
Assessing retinal ganglion cell death and neuroprotective agents using real time imaging. International-journal Peer-reviewed

Azusa Ito, Satoru Tsuda, Hiroshi Kunikata, Asano Toshifumi, Kota Sato, Toru Nakazawa

Brain research　1714　65-72　2019/07/01

DOI： 10.1016/j.brainres.2019.02.008 　

ISSN： 0006-8993

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The evaluation of retinal ganglion cell (RGC) death is a key part of retinal disease care. Previously, we used a Sytox Orange (SO)-based real-time imaging method to assess the RGCs in mice that underwent optic nerve crush. Here, we used N-methyl-D-aspartate (NMDA) injury in rats to confirm our model and assess the effect of neuroprotective agents on RGCs. The rats received NMDA injury and the intravitreal injection of SO, a cell-impermeant dyeing compound that targets nucleic acid. After ten minutes, non-invasive confocal scanning laser ophthalmoscopy visualized damaged or dying cells. Finally, the retinas were flat-mounted for histological confirmation of RGC death, with retrograde Fluorogold labeling and Alexa Fluor 488 Annexin V-conjugate (Annexin V) staining. This also revealed the time course of retinal cell death and the neuroprotective effect of SNJ-1945. Real-time imaging showed that SO-positive cells significantly increased starting 2 h after NMDA injection and reached an approximate plateau at 3 h. SO-positive cells were positive for Fluorogold and Annexin V in the isolated retinas. Moreover, the number of SO-positive retinal cells was significantly lower after treatment with SNJ-1945, compared to carboxymethyl cellulose. These results were confirmed in the isolated retinas. Thus, real-time imaging with SO allows the quick quantification of NMDA-induced RGC damage and death, and evaluation of neuroprotective agents. This technique may aid research into the development of new neuroprotective therapies.
Sectoral Differences in the Association of Optic Nerve Head Blood Flow and Glaucomatous Visual Field Defect Severity and Progression. International-journal Peer-reviewed

Naoki Kiyota, Yukihiro Shiga, Masayuki Yasuda, Naoko Aizawa, Kazuko Omodaka, Satoru Tsuda, Hiroshi Kunikata, Toru Nakazawa

Investigative ophthalmology & visual science　60　(7)　2650-2658　2019/06/03

DOI： 10.1167/iovs.19-27230 　

ISSN： 0146-0404

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Purpose: To investigate sectoral differences in the relationship between optic nerve head tissue blood flow, anatomically corresponding visual field defect severity, and future progression. Methods: This retrospective longitudinal medical chart review comprised 508 eyes of 319 open-angle glaucoma patients (mean deviation: -9.2 ± 7.0 dB), followed for an average of 4.7 ± 1.1 years; an average 11.7 ± 3.7 visual field tests were performed. Average total deviation (TD) was calculated in the superior, central, and inferior sectors of the Humphrey 24-2 program. The optic nerve head was divided to obtain inferior, temporal, and superior tissue-area mean blur rate (MT), derived from laser speckle flowgraphy. At baseline, the correlation between MT and TD was compared in anatomically corresponding sectors. We performed a multivariate analysis to determine the contribution of baseline MT to corresponding TD slope and to determine background factors influencing superior to temporal MT. We used a linear-mixed effect model for the statistical analysis. Results: At baseline, the highest β coefficients were found between MT-superior and TD-inferior, MT-temporal and TD-central, and between MT-inferior and TD-superior, in that order (β = 0.38, β = 0.27, β = 0.26, respectively). MT-superior and MT-temporal independently contributed to corresponding TD slope (P < 0.05). Male sex, high body mass index, and the prevalence of sleep apnea syndrome were contributing factors to lower superior to temporal MT (P < 0.05). Conclusions: Review of medical history, measurements of systemic variables, and laser speckle flowgraphy parameters might help clinicians to predict visual field defect severity and progression.
Glaucoma Diagnosis with Machine Learning Based on Optical Coherence Tomography and Color Fundus Images. International-journal Peer-reviewed

Guangzhou An, Kazuko Omodaka, Kazuki Hashimoto, Satoru Tsuda, Yukihiro Shiga, Naoko Takada, Tsutomu Kikawa, Hideo Yokota, Masahiro Akiba, Toru Nakazawa

Journal of healthcare engineering　2019　4061313-4061313　2019

DOI： 10.1155/2019/4061313 　

ISSN： 2040-2295

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This study aimed to develop a machine learning-based algorithm for glaucoma diagnosis in patients with open-angle glaucoma, based on three-dimensional optical coherence tomography (OCT) data and color fundus images. In this study, 208 glaucomatous and 149 healthy eyes were enrolled, and color fundus images and volumetric OCT data from the optic disc and macular area of these eyes were captured with a spectral-domain OCT (3D OCT-2000, Topcon). Thickness and deviation maps were created with a segmentation algorithm. Transfer learning of convolutional neural network (CNN) was used with the following types of input images: (1) fundus image of optic disc in grayscale format, (2) disc retinal nerve fiber layer (RNFL) thickness map, (3) macular ganglion cell complex (GCC) thickness map, (4) disc RNFL deviation map, and (5) macular GCC deviation map. Data augmentation and dropout were performed to train the CNN. For combining the results from each CNN model, a random forest (RF) was trained to classify the disc fundus images of healthy and glaucomatous eyes using feature vector representation of each input image, removing the second fully connected layer. The area under receiver operating characteristic curve (AUC) of a 10-fold cross validation (CV) was used to evaluate the models. The 10-fold CV AUCs of the CNNs were 0.940 for color fundus images, 0.942 for RNFL thickness maps, 0.944 for macular GCC thickness maps, 0.949 for disc RNFL deviation maps, and 0.952 for macular GCC deviation maps. The RF combining the five separate CNN models improved the 10-fold CV AUC to 0.963. Therefore, the machine learning system described here can accurately differentiate between healthy and glaucomatous subjects based on their extracted images from OCT data and color fundus images. This system should help to improve the diagnostic accuracy in glaucoma.
The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits. International-journal Peer-reviewed

Taiki Kokubun, Kotaro Yamamoto, Kota Sato, Takahiro Akaishi, Atsushi Shimazaki, Masatsugu Nakamura, Yukihiro Shiga, Satoru Tsuda, Kazuko Omodaka, Toru Nakazawa

PloS one　14　(3)　e0213811　2019

DOI： 10.1371/journal.pone.0213811 　

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PURPOSE: To investigate the potential of colchicine to improve bleb function after trabeculectomy. METHODS: To find the maximum usable colchicine concentration, an ocular irritation study was performed with the Draize test at concentrations of 0.001%, 0.01% and 0.1%. Additionally, the synergistic effect of topical colchicine instillation and MMC application to surgical site was evaluated in a rabbit model by measuring changes after trabeculectomy in intraocular pressure (IOP) and bleb morphology score at 3, 7, 14, 21, 28, 35, 42, and 49 days. RESULTS: Experiments with a rabbit model of trabeculectomy showed that 0.04% MMC plus 0.01% colchicine was more effective than saline and 0.04% MMC alone in maintaining IOP reduction at days 7-49 (P < 0.01 at all time points) and day 49 (P < 0.05), respectively, while 0.04% MMC alone was more effective than saline only at days 7-35 (P < 0.05 at all time points). 0.04% MMC plus 0.01% colchicine and 0.04% MMC alone were more effective than saline at preserving bleb score at days 7-21 and 35-49 (P < 0.05 at all time points) and at days 7-35 (P < 0.05 at all time points), respectively. CONCLUSION: Colchicine may be a promising adjuvant for strengthening the effect of MMC and improving the survival of the filtering bleb in trabeculectomy.
Prediction of Surgical Outcome After Trabeculectomy for Neovascular Glaucoma With Anterior-segment Optical Coherence Tomography. International-journal Peer-reviewed

Taiki Kokubun, Satoru Tsuda, Hiroshi Kunikata, Noriko Himori, Yu Yokoyama, Kazuko Omodaka, Shiho Kunimatsu-Sanuki, Toru Nakazawa

Journal of glaucoma　27　(12)　1157-1164　2018/12

DOI： 10.1097/IJG.0000000000001081 　

ISSN： 1057-0829

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PURPOSE: To determine the potential of anterior-segment optical coherence tomography (AS-OCT) to predict posttrabeculectomy outcomes in neovascular glaucoma (NVG). PATIENTS AND METHODS: We recruited 45 eyes of 40 NVG patients who underwent trabeculectomy. The patients were divided into success and failure groups based on the posttrabeculectomy outcome after 12 months. AS-OCT findings were compared in the success and failure groups at 1 and 2 weeks and 1, 3, 6, and 12 months. We also analyzed early posttrabeculectomy bleb parameters with multiple logistic regression, stepwise multiple regression, and the receiver operating characteristic curve to evaluate the power of these parameters to predict long-term outcomes. RESULTS: Intraocular pressure at 6 and 12 months was lower in the success group than the failure group (P<0.0016=0.0048). Reflectivity of the bleb wall was higher in the failure group than the success group throughout the study (all: P<0.0016). Age and reflectivity of the bleb wall at 1 week were risk factors for failure with odds ratios for failure of 0.91 and 1.67. The area under the receiver operating characteristic curve for reflectivity of the bleb wall at 1 week was 0.943 with the cutoff set at 128.9 (sensitivity: 85.7%; specificity: 100.0%). Stepwise multiple regression analysis showed that reflectivity of the bleb wall at 1 week independently indicated the bleb survival period (β=-0.84; P<0.001). CONCLUSIONS: AS-OCT-measured reflectivity of the bleb wall, measured at 1 week of trabeculectomy for NVG, might be able to predict of the final status of the bleb.
Association between mitochondrial DNA damage and ocular blood flow in patients with glaucoma. Peer-reviewed

Inoue-Yanagimachi M, Himori N, Sato K, Kokubun T, Asano T, Shiga Y, Tsuda S, Kunikata H, Nakazawa T

The British journal of ophthalmology　103　(8)　1060-1065　2018/09

DOI： 10.1136/bjophthalmol-2018-312356 　

ISSN： 0007-1161

eISSN： 1468-2079
Ecel1 Knockdown With an AAV2-Mediated CRISPR/Cas9 System Promotes Optic Nerve Damage-Induced RGC Death in the Mouse Retina. International-journal Peer-reviewed

Kota Sato, Yukihiro Shiga, Yurika Nakagawa, Kosuke Fujita, Koji M Nishiguchi, Hiroshi Tawarayama, Namie Murayama, Shigeto Maekawa, Takeshi Yabana, Kazuko Omodaka, Shota Katayama, Qiwei Feng, Satoru Tsuda, Toru Nakazawa

Investigative ophthalmology & visual science　59　(10)　3943-3951　2018/08/01

DOI： 10.1167/iovs.18-23784 　

ISSN： 0146-0404

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Purpose: To assess the therapeutic potential of endothelin-converting enzyme-like 1 (Ecel1) in a mouse model of optic nerve crush. Methods: Ecel1 expression was evaluated with real time quantitative (qRT)-PCR, Western blotting, and immunohistochemistry in mouse retinas after optic nerve crush. Vinblastine administration to the optic nerve and the intravitreal injection of N-methyl-d-aspartate (NMDA) were used to assess Ecel1 gene expression. Ecel1 was deleted with an adeno-associated viral (AAV) clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas9 system, and retinal ganglion cell (RGC) survival was investigated with retrograde labeling, qRT-PCR, and visual evoked potential. Results: Optic nerve crush induced Ecel1 expression specifically in the RGCs, peaking on day 4 after optic nerve crush. Ecel1 gene expression was induced by the vinblastine-induced inhibition of axonal flow, but not by NMDA-induced excitotoxicity, even though both are triggers of RGC death. Knockdown of Ecel1 promoted the loss of RGCs after optic nerve crush. Conclusions: Our data suggest that Ecel1 induction is part of the retinal neuroprotective response to axonal injury in mice. These findings might provide insight into novel therapeutic targets for the attenuation of RGC damage, such as occurs in traumatic optic neuropathy.
Anterior-Segment Optical Coherence Tomography for Predicting Postoperative Outcomes After Trabeculectomy. International-journal Peer-reviewed

Taiki Kokubun, Satoru Tsuda, Hiroshi Kunikata, Noriko Himori, Yu Yokoyama, Shiho Kunimatsu-Sanuki, Toru Nakazawa

Current eye research　43　(6)　762-770　2018/06

DOI： 10.1080/02713683.2018.1446535 　

ISSN： 0271-3683

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PURPOSE: To determine whether anterior-segment optical coherence tomography (AS-OCT) can be used to predict post-trabeculectomy bleb outcomes. MATERIALS AND METHODS: We divided 58 eyes of 47 trabeculectomy patients into success or failure groups based on their status at 12 months after surgery. We then compared various AS-OCT measurement parameters between the two groups at 1 and 2 weeks and 1, 3, 6, and 12 months. We also analyzed the early post-trabeculectomy bleb parameters with multiple logistic regression, stepwise multiple regression, and the receiver operating characteristic (ROC) curve, to evaluate the power of these parameters to predict long-term outcomes. RESULTS: Intraocular pressure 3 or more months after trabeculectomy was significantly lower in the success group than the failure group (all: P < 0.0016). Cleft volume was significantly higher 6 or more months after trabeculectomy in the success group than the failure group (P = 0.0027 and <0.0016). Reflectivity of the bleb wall was significantly higher in the failure group than the success group at 2 weeks and all later time points (all: P < 0.0016). Reflectivity of the bleb wall at 2 weeks after trabeculectomy was a risk factor for failure, with an odds ratio (OR) for failure of 2.48 (95% confidence interval, 1.31-4.68, increasing per 10 AU). The area under the ROC curve for reflectivity of the bleb wall at 2 weeks after trabeculectomy was 0.775 when the cutoff value was set at 122.8, with sensitivity, specificity, and OR of 78.3%, 80.0%, and 14.4, respectively. A stepwise multiple regression analysis showed that reflectivity of the bleb wall at 2 weeks was an independent factor indicating postoperative bleb survival period (β = -0.39, P = 0.007). CONCLUSIONS: Reflectivity of the bleb wall, measured by AS-OCT, may be an early post-trabeculectomy predictor of bleb outcome.
Pilot study for three-dimensional assessment of laminar pore structure in patients with glaucoma, as measured with swept source optical coherence tomography. International-journal Peer-reviewed

Kazuko Omodaka, Shigeto Maekawa, Guangzhou An, Satoru Tsuda, Yukihiro Shiga, Naoko Takada, Tsutomu Kikawa, Hidetoshi Takahashi, Hideo Yokota, Masahiro Akiba, Toru Nakazawa

PloS one　13　(11)　e0207600　2018

DOI： 10.1371/journal.pone.0207600 　

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PURPOSE: To develop a method to quantify, based on swept-source optical coherence tomography (OCT), the 3D structure of the laminar pores in patients with glaucoma. METHODS: This retrospective study examined 160 laminar pores from 8 eyes of 8 cases: 4 normal subjects and 4 open-angle glaucoma (OAG) patients. We reconstructed 3D volume data for a 3 x 3 mm disc, using a method similar to OCT angiography, and segmented the structure of the lamina cribrosa. Then, we manually segmented each laminar pore in sequential C-scan images (>90 slices at 2.6-micron intervals) with VCAT5 (RIKEN, Japan). We compared the control and OAG subjects with the Mann-Whitney U test. Differences were considered significant at p < 0.05. RESULTS: We found that the laminar pores of the OAG patients had a significantly smaller average cross-sectional area, smaller 3D volume (adjusted to the average thickness of the lamina cribrosa), and higher true sphericity, and lower principal value (P1, 2, 3) of the 3D structure data (all: p < 0.0001). The topographic distribution of damaged laminar pores was consistent with the damaged area of the macular map. CONCLUSION: We successfully developed a method to quantify the 3D structure of the laminar pores; providing a useful tool to assess lamina cribrosa-associated risk factors for glaucoma. These findings promise to benefit future investigations into the pathomechanisms of glaucoma.
Characteristic Profiles of Inflammatory Cytokines in the Aqueous Humor of Glaucomatous Eyes. International-journal Peer-reviewed

Taiki Kokubun, Satoru Tsuda, Hiroshi Kunikata, Masayuki Yasuda, Noriko Himori, Shiho Kunimatsu-Sanuki, Kazuichi Maruyama, Toru Nakazawa

Ocular immunology and inflammation　26　(8)　1177-1188　2018

DOI： 10.1080/09273948.2017.1327605 　

ISSN： 0927-3948

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PURPOSE: To analyze cytokine profiles of the aqueous humor of eyes with primary open-angle glaucoma (POAG), neovascular glaucoma (NVG), and cataract (as controls). METHODS: A multiplex bead assay was used to measure concentrations of 27 cytokines in aqueous humor samples from 54 eyes. RESULTS: Detection rates were as follows: IL-7: NVG higher than POAG; IL-10: POAG lower than cataract or NVG; and GM-CSF: cataract higher than POAG or NVG. Concentrations were as follows: IL-8, IP-10, MCP-1, and MIP-1β: POAG and NVG higher than cataract; IL-9: POAG lower than NVG; IL-12: POAG lower than cataract or NVG; and VEGF: NVG higher than cataract or POAG and POAG lower than cataract. Further analysis showed that IL-8, IP-10, MCP-1, and MIP-1β were correlated with intraocular pressure and age. CONCLUSIONS: The detection rates and levels of various cytokines had different patterns in POAG and NVG patients, suggesting distinctive alterations in the microenvironment in different types of glaucoma.
Preperimetric Glaucoma Prospective Study (PPGPS): Predicting Visual Field Progression With Basal Optic Nerve Head Blood Flow in Normotensive PPG Eyes. International-journal Peer-reviewed

Yukihiro Shiga, Naoko Aizawa, Satoru Tsuda, Yu Yokoyama, Kazuko Omodaka, Hiroshi Kunikata, Tomoki Yasui, Keiichi Kato, Hiroaki Kurashima, Etsuyo Miyamoto, Masayo Hashimoto, Toru Nakazawa

Translational vision science & technology　7　(1)　11-11　2018/01

DOI： 10.1167/tvst.7.1.11 　

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Purpose: To investigate the site specificity of visual field changes in eyes with normotensive preperimetric glaucoma (PPG), and to determine factors influencing visual field progression. Methods: This prospective study comprised 84 eyes of 84 normotensive PPG patients followed for at least 16 months. Optic nerve head (ONH) blood flow was assessed with tissue-area mean blur rate (MBRT), derived from laser speckle flowgraphy. Total deviation (TD) was measured in each sector of the Garway-Heath map to evaluate the site specificity of visual field loss. Subjects with a TD slope in the first quartile were classified as progressive, and other subjects as nonprogressive. Linear and multiple regression analyses were performed to determine factors affecting visual field progression. Results: TD in the superior sector significantly decreased in the subjects overall during the follow-up periods (-0.48 ± 1.92 dB/y, P = 0.025). Linear regression analysis showed that basal MBRT-inferior was correlated significantly with TD-superior slope (r = 0.332, P = 0.002). Furthermore, basal MBRT was significantly lower in this sector in the progressive than the nonprogressive group (P = 0.010). Multiple linear regression analysis revealed that basal MBRT-inferior was the only predictive factor for TD-superior slope (β = 0.329, P = 0.005). Conclusions: These findings suggest that superior-sector visual field progression is most common in normotensive PPG eyes, and that reduced basal ONH blood flow is associated with visual field progression. Translational Relevance: These findings provide new insight into the involvement of ONH blood flow impairment in glaucoma pathogenesis, and demonstrate the importance of assessing ONH blood flow to determine visual field progression in normotensive PPG.
Comparison of Machine-Learning Classification Models for Glaucoma Management. International-journal Peer-reviewed

Guangzhou An, Kazuko Omodaka, Satoru Tsuda, Yukihiro Shiga, Naoko Takada, Tsutomu Kikawa, Toru Nakazawa, Hideo Yokota, Masahiro Akiba

Journal of healthcare engineering　2018　6874765-6874765　2018

DOI： 10.1155/2018/6874765 　

ISSN： 2040-2295

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This study develops an objective machine-learning classification model for classifying glaucomatous optic discs and reveals the classificatory criteria to assist in clinical glaucoma management. In this study, 163 glaucoma eyes were labelled with four optic disc types by three glaucoma specialists and then randomly separated into training and test data. All the images of these eyes were captured using optical coherence tomography and laser speckle flowgraphy to quantify the ocular structure and blood-flow-related parameters. A total of 91 parameters were extracted from each eye along with the patients' background information. Machine-learning classifiers, including the neural network (NN), naïve Bayes (NB), support vector machine (SVM), and gradient boosted decision trees (GBDT), were trained to build the classification models, and a hybrid feature selection method that combines minimum redundancy maximum relevance and genetic-algorithm-based feature selection was applied to find the most valid and relevant features for NN, NB, and SVM. A comparison of the performance of the three machine-learning classification models showed that the NN had the best classification performance with a validated accuracy of 87.8% using only nine ocular parameters. These selected quantified parameters enabled the trained NN to classify glaucomatous optic discs with relatively high performance without requiring color fundus images.
Usefulness of axonal tract-dependent OCT macular sectors for evaluating structural change in normal-tension glaucoma. International-journal Peer-reviewed

Kazuko Omodaka, Tsutomu Kikawa, Yukihiro Shiga, Satoru Tsuda, Yu Yokoyama, Haruka Sato, Junko Ohuchi, Akiko Matsumoto, Hidetoshi Takahashi, Masahiro Akiba, Toru Nakazawa

PloS one　12　(10)　e0185649　2017

DOI： 10.1371/journal.pone.0185649 　

ISSN： 1932-6203
Preperimetric Glaucoma Prospective Observational Study (PPGPS): Design, baseline characteristics, and therapeutic effect of tafluprost in preperimetric glaucoma eye. International-journal Peer-reviewed

Naoko Aizawa, Hiroshi Kunikata, Yukihiro Shiga, Satoru Tsuda, Yu Yokoyama, Kazuko Omodaka, Tomoki Yasui, Keiichi Kato, Hiroaki Kurashima, Etsuyo Miyamoto, Masayo Hashimoto, Toru Nakazawa

PloS one　12　(12)　e0188692　2017

DOI： 10.1371/journal.pone.0188692 　

ISSN： 1932-6203
Classification of optic disc shape in glaucoma using machine learning based on quantified ocular parameters. International-journal Peer-reviewed

Kazuko Omodaka, Guangzhou An, Satoru Tsuda, Yukihiro Shiga, Naoko Takada, Tsutomu Kikawa, Hidetoshi Takahashi, Hideo Yokota, Masahiro Akiba, Toru Nakazawa

PloS one　12　(12)　e0190012　2017

DOI： 10.1371/journal.pone.0190012 　

ISSN： 1932-6203
Erratum: Estimation of Jones matrix, birefringence and entropy using Cloude-Pottier decomposition in polarization-sensitive optical coherence tomography: erratum. International-journal Peer-reviewed

Masahiro Yamanari, Satoru Tsuda, Taiki Kokubun, Yukihiro Shiga, Kazuko Omodaka, Naoko Aizawa, Yu Yokoyama, Noriko Himori, Shiho Kunimatsu-Sanuki, Kazuichi Maruyama, Hiroshi Kunikata, Toru Nakazawa

Biomedical optics express　7　(11)　4636-4638　2016/11/01

DOI： 10.1364/BOE.7.004636 　

ISSN： 2156-7085
Estimation of Jones matrix, birefringence and entropy using Cloude-Pottier decomposition in polarization-sensitive optical coherence tomography. International-journal Peer-reviewed

Masahiro Yamanari, Satoru Tsuda, Taiki Kokubun, Yukihiro Shiga, Kazuko Omodaka, Naoko Aizawa, Yu Yokoyama, Noriko Himori, Shiho Kunimatsu-Sanuki, Kazuichi Maruyama, Hiroshi Kunikata, Toru Nakazawa

Biomedical optics express　7　(9)　3551-3573　2016/09/01

DOI： 10.1364/BOE.7.003551 　

ISSN： 2156-7085
Quantification of the filtering bleb's structure with anterior segment optical coherence tomography. International-journal Peer-reviewed

Taiki Kokubun, Hiroshi Kunikata, Satoru Tsuda, Noriko Himori, Kazuichi Maruyama, Toru Nakazawa

Clinical & experimental ophthalmology　44　(6)　446-54　2016/08

DOI： 10.1111/ceo.12689 　

ISSN： 1442-6404

eISSN： 1442-9071
Transcriptome profiling of the rat retina after optic nerve transection. International-journal Peer-reviewed

Masayuki Yasuda, Yuji Tanaka, Kazuko Omodaka, Koji M Nishiguchi, Orie Nakamura, Satoru Tsuda, Toru Nakazawa

Scientific reports　6　28736-28736　2016/06/29

DOI： 10.1038/srep28736 　

ISSN： 2045-2322
Real-time imaging of RGC death with a cell-impermeable nucleic acid dyeing compound after optic nerve crush in a murine model. International-journal Peer-reviewed

Satoru Tsuda, Yuji Tanaka, Hiroshi Kunikata, Yu Yokoyama, Masayuki Yasuda, Azusa Ito, Toru Nakazawa

Experimental eye research　146　179-188　2016/05

DOI： 10.1016/j.exer.2016.03.017 　

ISSN： 0014-4835

eISSN： 1096-0007
Age- and Sex-Dependency of Laser Speckle Flowgraphy Measurements of Optic Nerve Vessel Microcirculation. International-journal Peer-reviewed

Naoko Aizawa, Hiroshi Kunikata, Fumihiko Nitta, Yukihiro Shiga, Kazuko Omodaka, Satoru Tsuda, Toru Nakazawa

PloS one　11　(2)　e0148812　2016

DOI： 10.1371/journal.pone.0148812 　

ISSN： 1932-6203
In vivo cellular imaging of various stress/response pathways using AAV following axonal injury in mice. International-journal Peer-reviewed

Kosuke Fujita, Koji M Nishiguchi, Yu Yokoyama, Yusuke Tomiyama, Satoru Tsuda, Masayuki Yasuda, Shigeto Maekawa, Toru Nakazawa

Scientific reports　5　18141-18141　2015/12/16

DOI： 10.1038/srep18141 　

ISSN： 2045-2322
Association between histological findings and polarization-sensitive optical coherence tomography analysis of a post-trabeculectomy human eye Peer-reviewed

Satoru Tsuda, Hiroshi Kunikata, Masahiro Yamanari, Toru Nakazawa

CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY　43　(7)　685-688　2015/09

DOI： 10.1111/ceo.12522 　

ISSN： 1442-6404

eISSN： 1442-9071
Topographical correlation between macular layer thickness and clockwise circumpapillary retinal nerve fiber layer sectors in patients with normal tension glaucoma. International-journal Peer-reviewed

Kazuko Omodaka, Yu Yokoyama, Yukihiro Shiga, Maki Inoue, Seri Takahashi, Satoru Tsuda, Kazuichi Maruyama, Toru Nakazawa

Current eye research　40　(7)　744-51　2015/07

DOI： 10.3109/02713683.2014.956371 　

ISSN： 0271-3683

eISSN： 1460-2202
Molecular, anatomical and functional changes in the retinal ganglion cells after optic nerve crush in mice. International-journal Peer-reviewed

Masayoshi Yukita, Shigeki Machida, Koji M Nishiguchi, Satoru Tsuda, Yu Yokoyama, Masayuki Yasuda, Kazuichi Maruyama, Toru Nakazawa

Documenta ophthalmologica. Advances in ophthalmology　130　(2)　149-56　2015/04

DOI： 10.1007/s10633-014-9478-2 　

ISSN： 0012-4486

eISSN： 1573-2622
眼疾患メカニズムの新しい理解失明ゼロを目指して

中澤徹, 國方彦志, 横倉俊二, 丸山和一, 西口康二, 高橋秀肇, 国松志保, 劉孟林, 檜森紀子, 田中佑治, 新田文彦, 森藤暁, 藤田幸輔, 中村織江, 面高宗子, 安田正幸, 横山悠, 相澤奈帆子, 山本耕太郎, 雪田昌克, 津田聡, 志賀由己浩, 前川重人, 國分太貴, 今野瑛之, 高橋麻衣, 萱場寛子, 橋本清香, 佐藤茉莉華, 矢花武史, 高田菜生子, 高橋世理, 鎌田泰彰

日本眼科学会雑誌　119　(3)　168-194　2015/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
Fiber-based polarization-sensitive OCT for birefringence imaging of the anterior eye segment. International-journal Peer-reviewed

Masahiro Yamanari, Satoru Tsuda, Taiki Kokubun, Yukihiro Shiga, Kazuko Omodaka, Yu Yokoyama, Noriko Himori, Morin Ryu, Shiho Kunimatsu-Sanuki, Hidetoshi Takahashi, Kazuichi Maruyama, Hiroshi Kunikata, Toru Nakazawa

Biomedical optics express　6　(2)　369-89　2015/02/01

DOI： 10.1364/BOE.6.000369 　

ISSN： 2156-7085
Pulse-waveform analysis of normal population using laser speckle flowgraphy. International-journal Peer-reviewed

Satoru Tsuda, Hiroshi Kunikata, Masahiko Shimura, Naoko Aizawa, Kazuko Omodaka, Yukihiro Shiga, Masayuki Yasuda, Yu Yokoyama, Toru Nakazawa

Current eye research　39　(12)　1207-15　2014/12

DOI： 10.3109/02713683.2014.905608 　

ISSN： 0271-3683

eISSN： 1460-2202
Retinal transcriptome profiling at transcription start sites: a cap analysis of gene expression early after axonal injury. International-journal Peer-reviewed

Masayuki Yasuda, Yuji Tanaka, Koji M Nishiguchi, Morin Ryu, Satoru Tsuda, Kazuichi Maruyama, Toru Nakazawa

BMC genomics　15　982-982　2014/11/18

DOI： 10.1186/1471-2164-15-982 　

ISSN： 1471-2164
【濾過手術後眼を科学する】トラベクレクトミー後の濾過胞瘢痕化とその評価

津田聡, 中澤徹

眼科手術　27　(4)　491-498　2014/10
Publisher: (公社)日本眼科手術学会
ISSN： 0914-6806
Profiles of extracellular miRNAs in the aqueous humor of glaucoma patients assessed with a microarray system. International-journal Peer-reviewed

Yuji Tanaka, Satoru Tsuda, Hiroshi Kunikata, Junko Sato, Taiki Kokubun, Masayuki Yasuda, Koji M Nishiguchi, Toshifumi Inada, Toru Nakazawa

Scientific reports　4　5089-5089　2014/05/28

DOI： 10.1038/srep05089 　

ISSN： 2045-2322
眼疾患と遺伝子緑内障のゲノム解析次世代医療・個別化医療に向けて

布施昇男, 清水愛, 木村雅恵, 高野良真, 石棟, 宮澤晃子, 国松志保, 劉孟林, 渡邉亮, 安田正幸, 横山悠, 檜森紀子, 津田聡, 山本耕太郎, 中澤徹, 安田純, 勝岡史城, 小島要, 成相直樹, 松本光代, 元池育子, 長崎正朗, 木下賢吾, 五十嵐和彦, 山本雅之, 新堀哲也, 青木洋子, 松原洋一, 舟山亮, 長嶋剛史, 中山啓子, 眞島行彦, 船山智代, 田中光一, 原田高幸, 阿部春樹, 福地健郎, 安田典子, 出田秀尚, 鄭暁東, 白石敦, 大橋裕一, 石田誠夫, 原岳, 金森章泰, 山田裕子, 中村誠, 酒井寛, Richards Julia E

日本眼科学会雑誌　118　(3)　216-240　2014/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
RNA sequence reveals mouse retinal transcriptome changes early after axonal injury. International-journal Peer-reviewed

Masayuki Yasuda, Yuji Tanaka, Morin Ryu, Satoru Tsuda, Toru Nakazawa

PloS one　9　(3)　e93258　2014

DOI： 10.1371/journal.pone.0093258 　

ISSN： 1932-6203
Stereoscopic analysis of optic nerve head parameters in primary open angle glaucoma: the glaucoma stereo analysis study. International-journal Peer-reviewed

Yu Yokoyama, Masaki Tanito, Koji Nitta, Maki Katai, Yasushi Kitaoka, Kazuko Omodaka, Satoru Tsuda, Toshiaki Nakagawa, Toru Nakazawa

PloS one　9　(6)　e99138　2014

DOI： 10.1371/journal.pone.0099138 　

ISSN： 1932-6203
Characteristics of uveitic glaucoma and evaluation of its surgical treatment. International-journal Peer-reviewed

Ai Shimizu, Kazuichi Maruyama, Yu Yokoyama, Satoru Tsuda, Morin Ryu, Toru Nakazawa

Clinical ophthalmology (Auckland, N.Z.)　8　2383-9　2014
Publisher: Dove Medical Press Ltd
DOI： 10.2147/OPTH.S72383 　

ISSN： 1177-5483 1177-5467
Development of azo-based fluorescent probes to detect different levels of hypoxia. International-journal Peer-reviewed

Wen Piao, Satoru Tsuda, Yuji Tanaka, Satoshi Maeda, Fengyi Liu, Shodai Takahashi, Yu Kushida, Toru Komatsu, Tasuku Ueno, Takuya Terai, Toru Nakazawa, Masanobu Uchiyama, Keiji Morokuma, Tetsuo Nagano, Kenjiro Hanaoka

Angewandte Chemie (International ed. in English)　52　(49)　13028-32　2013/12/02

DOI： 10.1002/anie.201305784 　

ISSN： 1433-7851 1521-3773
Waveform analysis of ocular blood flow and the early detection of normal tension glaucoma. International-journal Peer-reviewed

Yukihiro Shiga, Kazuko Omodaka, Hiroshi Kunikata, Morin Ryu, Yu Yokoyama, Satoru Tsuda, Toshifumi Asano, Shigeto Maekawa, Kazuichi Maruyama, Toru Nakazawa

Investigative ophthalmology & visual science　54　(12)　7699-706　2013/11/21

DOI： 10.1167/iovs.13-12930 　

ISSN： 0146-0404

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PURPOSE: To investigate waveform changes in blood flow (BF) in the optic nerve head (ONH) and to evaluate their usefulness in identifying normal tension glaucoma (NTG). METHODS: Sixty-one eyes of 61 patients with NTG and 21 eyes of age-matched healthy control subjects were included in this study. The NTG eyes were divided into the following three groups based on the progression of their visual field defects: mild (mean deviation [MD] greater than -6.0 decibels [dB]), moderate (MD between -6.0 and -12.0 dB), and severe (MD less than -12.0 dB). The ONH BF analysis was performed with laser speckle flowgraphy (LSFG) and included waveform variables such as skew, acceleration time index (ATI), and blowout time. RESULTS: In the ONH, LSFG skew variables were significantly lower in the NTG eyes than in the control eyes (P < 0.001), and ATI was significantly higher (P < 0.01), despite similar systemic characteristics in the four groups. The differences were most marked in the mild NTG group. Multiple linear regression analysis showed that MD, average thickness of the circumpapillary retinal nerve fiber layer, and pulse rate were predictive factors for both skew and ATI. A receiver operating characteristic (ROC) curve analysis also revealed that skew (area under the ROC curve, 0.89) and ATI (area under the ROC curve, 0.80) had the greatest power to differentiate normal eyes from eyes with mild NTG. CONCLUSIONS: These results suggest that LSFG measurements of waveform changes in ONH BF can differentiate healthy eyes from eyes with NTG, particularly those with mild NTG.
ステレオ眼底カメラによる緑内障性視神経乳頭障害指標(DDLS)の算出

北岡康史, 谷戸正樹, 新田耕治, 片井麻貴, 横山悠, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　60-60　2013/09
Publisher: 日本緑内障学会
ステレオ眼底カメラにより計測した視神経乳頭形状パラメーターを用いた乳頭形状新分類

谷戸正樹, 新田耕治, 片井麻貴, 北岡康史, 横山悠, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　60-60　2013/09
Publisher: 日本緑内障学会
ステレオ眼底カメラにより計測した視神経乳頭形状パラメーターによる乳頭形状4群分類

新田耕治, 谷戸正樹, 片井麻貴, 北岡康史, 横山悠, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　61-61　2013/09
Publisher: 日本緑内障学会
Development of a new strategy of visual field testing for macular dysfunction in patients with open angle glaucoma. Peer-reviewed

Kazuko Omodaka, Shiho Kunimatsu-Sanuki, Ryu Morin, Satoru Tsuda, Yu Yokoyama, Hidetoshi Takahashi, Kazuichi Maruyama, Hiroshi Kunikata, Toru Nakazawa

Japanese journal of ophthalmology　57　(5)　457-62　2013/09

DOI： 10.1007/s10384-013-0258-4 　

ISSN： 0021-5155
Effect of Topical Tafluprost on Optic Nerve Head Blood Flow in Patients With Myopic Disc Type Peer-reviewed

Satoru Tsuda, Yu Yokoyama, Naoki Chiba, Naoko Aizawa, Yukihiro Shiga, Masayuki Yasuda, Shunji Yokokura, Takaaki Otomo, Nobuo Fuse, Toru Nakazawa

JOURNAL OF GLAUCOMA　22　(5)　398-403　2013/06

DOI： 10.1097/IJG.0b013e318237c8b3 　

ISSN： 1057-0829
The influence of posture change on ocular blood flow in normal subjects, measured by laser speckle flowgraphy. International-journal Peer-reviewed

Yukihiro Shiga, Masahiko Shimura, Toshifumi Asano, Satoru Tsuda, Yu Yokoyama, Naoko Aizawa, Kazuko Omodaka, Morin Ryu, Shunji Yokokura, Takayuki Takeshita, Toru Nakazawa

Current eye research　38　(6)　691-8　2013/06

DOI： 10.3109/02713683.2012.758292 　

ISSN： 0271-3683
後期緑内障患者における視野進行評価法 Peer-reviewed

渡邉亮, 国松志保, 横山悠, 今野瑛之, 浅野俊文, 津田聡, 面高宗子, 劉孟林, 高橋秀肇, 中澤徹

日本眼科学会雑誌　117　(臨増)　271-271　2013/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
OCTによる12分割乳頭周囲網膜神経線維層厚に対応する視野検査点の検討 Peer-reviewed

今野瑛之, 国松志保, 横山悠, 面高宗子, 津田聡, 高橋麻衣, 浅野俊文, 渡邊亮, 劉孟林, 高橋秀肇, 丸山和一, 岩瀬愛子, 中澤徹

日本眼科学会雑誌　117　(臨増)　304-304　2013/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
角膜移植の歴史

上松聖典, 横倉俊二, 津田聡, 千葉真生, 津田知世子, 西田幸二

眼科　54　(13)　1915-1927　2012/12
Publisher: 金原出版(株)
ISSN： 0016-4488
ステレオ眼底カメラにより計測した視神経乳頭形状パラメーターの乳頭形状4分類間比較

新田耕治, 谷戸正樹, 片井麻貴, 北岡康史, 横山悠, 面高宗子, 津田聡, 中川俊明, 中澤徹

日本緑内障学会抄録集　23回　110-110　2012/09
Publisher: 日本緑内障学会
外傷性眼窩内血腫に対し経皮的眼窩内血腫除去術を行った1例

津田聡, 中村政彦, 土井洋, 布施昇男

臨床眼科　65　(7)　1143-1148　2011/07
Publisher: (株)医学書院
DOI： 10.11477/mf.1410103758 　

ISSN： 0370-5579

eISSN： 1882-1308
東北大学病院におけるぶどう膜炎の臨床統計

大友孝昭, 阿部俊明, 劉孟林, 渡邉亮, 津田聡, 岡村知世子, 千葉真生, 布施昇男

臨床眼科　65　(6)　891-894　2011/06
Publisher: (株)医学書院
DOI： 10.11477/mf.1410103698 　

ISSN： 0370-5579

eISSN： 1882-1308
Association between optic nerve blood flow and objective examinations in glaucoma patients with generalized enlargement disc type. International-journal Peer-reviewed

Naoki Chiba, Kazuko Omodaka, Yu Yokoyama, Naoko Aizawa, Satoru Tsuda, Masayuki Yasuda, Takaaki Otomo, Shunji Yokokura, Nobuo Fuse, Toru Nakazawa

Clinical ophthalmology (Auckland, N.Z.)　5　1549-56　2011

DOI： 10.2147/OPTH.S22097 　

ISSN： 1177-5467

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BACKGROUND: The purpose of this study was to investigate the correlations between microcirculation in the optic disc, average peripapillary retinal nerve fiber layer thickness cupping parameters, and visual field defects in glaucoma patients with the generalized enlargement disc type. METHODS: A total of 38 eyes from 38 glaucoma patients with the generalized enlargement disc type were included. The microcirculation of the optic nerve head was examined with laser speckle flow graphy, and the mean blur rate in all areas, in vessel area, and in tissue area were calculated using the laser speckle flow graphy analyzer software. Average peripapillary retinal nerve fiber layer thickness was measured using Stratus optical coherence tomography, and cupping parameters were accessed using the Heidelberg retina tomograph. The mean deviation in the Humphrey field analyzer (30-2 SITA standard) was analyzed. The correlation between these parameters was evaluated using the Spearman rank correlation coefficient. RESULTS: The correlation coefficient of mean blur rate in all optic disc area to the average peripapillary retinal nerve fiber layer thickness, vertical C/D, and mean deviation were r = 0.7546 (P < 0.0001), r = -0.6208 (P < 0.0001), and r = 0.6010 (P = 0.0001), respectively. The mean blur rate in tissue area of the optic disc showed r = 0.7305 (P < 0.0001), r = -0.6438 (P < 0.0001), and r = 0.6338 (P < 0.0001). CONCLUSION: We found that the mean blur rate in the optic disc was significantly correlated with the average peripapillary retinal nerve fiber layer thickness, vertical C/D, and mean deviation in patients with the generalized enlargement disc type of glaucoma. In particular, the mean blur rate in tissue area was more highly correlated than the vessel area with other results of examination in glaucoma patients with the generalized enlargement disc type.
[Ehlers-Danlos syndrome with a frequent intestinal perforation]. Peer-reviewed

Takayuki Sahata, Satoru Tsuda, Masaki Abo, Kazunori Sanekata, Toshio Yamagishi, Yoko Aoki, Tomoki Kosho, Atsushi Hatamochi

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine　98　(4)　852-4　2009/04/10

ISSN： 0021-5384

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Misc. 123

Detection of cataract with MEOCHECK, a simple visual field measurement device using retinal projection technology

Tadashi Nakashima, Takeshi Yabana, Masataka Sato, Takahiro Ninomiya, Mai Yamazaki, Noriko Himori, Morino Seiji, Makoto Suzuki, Satoru Tsuda, Yu Yokoyama, Makoto Ishikawa, Hiroshi Kunikata, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　65　(7)　2024/06

ISSN： 0146-0404

eISSN： 1552-5783
Phase 2a study of calpain inhibitor SJP-0008 for central retinal artery occlusion

Satoru Tsuda, Hiroshi Kunikata, Kazuki Hashimoto, Toshifumi Asano, Azusa Ito, Taiki Kokubun, Yoshida Mitsuhide, Masayuki Yasuda, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　65　(7)　2024/06

ISSN： 0146-0404

eISSN： 1552-5783
動脈炎性前部虚血性視神経症におけるLaser Speckle Flowgraphyの有用性

山口知暁, 檜森紀子, 檜森紀子, 清田直樹, 大島隆寛, 竹下孝之, 津田聡, 面高宗子, 中澤徹

日本眼循環学会講演抄録集　40th　2024
Comparison of Superior and Inferior Microvasculature Dropout in Glaucoma

高橋哲, 高橋直樹, 津田聡, 面高宗子, 中澤徹

日本眼科学会雑誌　128　2024

ISSN： 0029-0203
Association between disc hemorrhage and optic nerve head blood flow in glaucoma

高橋直樹, 面高宗子, 羽入田明子, 武田瑶子, 津田聡, 中澤徹

日本眼科学会雑誌　128　2024

ISSN： 0029-0203
視神経乳頭周囲組織血流に着目した緑内障における線維柱帯切除術後の視力低下

武田瑶子, 高橋直樹, 國分太貴, 津田聡, 面高宗子, 中澤徹

日本眼循環学会講演抄録集　39th　2023
Laser speckle flowgraphyを用いた視神経症の鑑別についての検討

山口知暁, 清田直樹, 檜森紀子, 津田聡, 面高宗子, 中澤徹

日本眼循環学会講演抄録集　39th　2023
CRAOを対象としたカルパイン阻害薬SJP-0008の医師主導第IIa相試験

津田聡, 國方彦志, 橋本和軌, 浅野俊文, 伊藤梓, 國分太貴, 吉田光秀, 安田正幸, 中澤徹

日本眼循環学会講演抄録集　39th　2023
サイトメガロウイルス陽性続発緑内障のトラベクレクトミー術後成績

吉田真彰, 横山悠, 國分太貴, 津田聡, 檜森紀子, 前川重人, 針谷威寛, 橋本和軌, 中澤徹

日本眼感染症学会・日本眼炎症学会・日本コンタクトレンズ学会総会・日本涙道・涙液学会プログラム・講演抄録集　58th-55th-64th-10th　2022
家兎でのマイクロパルス波経強膜毛様体光凝固術後の眼圧下降と組織障害の検討

津田聡, 國分太貴, 岡部達, 桐山卓也, 中澤徹

日本眼科学会雑誌　125　(臨増)　175-175　2021/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
正常眼圧白内障手術の角膜内皮保護効果に関する検証

國分太貴, 津田聡, 矢花武史, 前川重人, 横山悠, 國方彦志, 中澤徹

日本眼科学会雑誌　125　(臨増)　188-188　2021/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
正常眼圧緑内障の病期とcorneal hysteresisの関連

内田惠子, 檜森紀子, 國分太貴, 矢花武史, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本眼科学会雑誌　125　(臨増)　240-240　2021/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
広義原発開放隅角緑内障病型とFlammer問診票スコアの関連

高橋成奈, 志賀由己浩, 清田直樹, 安田正幸, 佐藤茉莉華, 津田聡, 檜森紀子, 面高宗子, 高山真, 石井正, 中澤徹

日本緑内障学会抄録集　31回　75-75　2020/10
Publisher: 日本緑内障学会
緑内障眼における偏光OCTによる強膜複屈折の評価

桐山卓也, 津田聡, 國分太貴, 山成正宏, 矢花武史, 前川重人, 横山悠, 中澤徹

日本緑内障学会抄録集　31回　145-145　2020/10
Publisher: 日本緑内障学会
新しい緑内障薬物治療急性緑内障発作用の神経保護治療の可能性

津田聡

日本緑内障学会抄録集　31回　155-155　2020/10
Publisher: 日本緑内障学会
緑内障患者における複合サプリメント摂取による酸化ストレス、抗酸化力の変動

檜森紀子, 柳町真希, 志賀由己浩, 面高宗子, 津田聡, 國方彦志, 中澤徹

日本抗加齢医学会総会プログラム・抄録集　20回　191-191　2020/09
Publisher: (一社)日本抗加齢医学会
近視を有する緑内障患者の3D T2強調MRIによる眼球形状と体積の定量的解析

面高宗子, 舘脇康子, 檜森紀子, 津田聡, 武藤達士, ティロー・バンジャマン, 松平泉, 川島隆太, 瀧靖之, 中澤徹

日本眼科学会雑誌　124　(臨増)　172-172　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
乳頭周囲網脈絡膜萎縮部血流と緑内障視野障害進行の関連

清田直樹, 志賀由己浩, 高橋成奈, 安田正幸, 面高宗子, 津田聡, 國方彦志, 中澤徹

日本眼科学会雑誌　124　(臨増)　172-172　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
ウサギ模擬白内障手術を用いたグルタチオン三硫化物(GS3G)による眼組織保護効果の検討

鈴木哲章, 國方彦志, 俵山寛司, 佐藤孝太, 津田聡, 中澤徹

日本眼科学会雑誌　124　(臨増)　183-183　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
家兎濾過手術モデルでの偏光OCTの瘢痕定量評価と組織学的所見の関連性

國分太貴, 津田聡, 浅野俊文, 鈴木哲章, 前川重人, 横山悠, 佐藤孝太, 中澤徹

日本眼科学会雑誌　124　(臨増)　214-214　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
Cornea hysteresisと濾過手術成績との関連

内田惠子, 檜森紀子, 國分太貴, 二宮高洋, 志賀由己浩, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本眼科学会雑誌　124　(臨増)　214-214　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
家兎におけるカルパイン活性の生体内イメージング

浅野俊文, 津田聡, 國方彦志, 神谷真子, 浦野泰照, 中澤徹

日本眼科学会雑誌　124　(臨増)　234-234　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
開放隅角緑内障患者の前房水メタボローム解析

横山悠, 佐藤孝太, 三枝大輔, 藤岡周, 國分太貴, 前川重人, 津田聡, 面高宗子, 中澤徹

日本眼科学会雑誌　124　(臨増)　238-238　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
近視を有する緑内障患者の3D T2強調MRIによる眼球形状と体積の定量的解析

面高宗子, 舘脇康子, 檜森紀子, 津田聡, 武藤達士, ティロー・バンジャマン, 松平泉, 川島隆太, 瀧靖之, 中澤徹

日本眼科学会雑誌　124　(臨増)　172-172　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
乳頭周囲網脈絡膜萎縮部血流と緑内障視野障害進行の関連

清田直樹, 志賀由己浩, 高橋成奈, 安田正幸, 面高宗子, 津田聡, 國方彦志, 中澤徹

日本眼科学会雑誌　124　(臨増)　172-172　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
マイクロパルス波経強膜毛様体光凝固術後の6ヵ月間成績

小杉真代, 津田聡, 國分太貴, 前川重人, 横山悠, 中澤徹

日本眼科学会雑誌　124　(臨増)　176-176　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
ウサギ模擬白内障手術を用いたグルタチオン三硫化物(GS3G)による眼組織保護効果の検討

鈴木哲章, 國方彦志, 俵山寛司, 佐藤孝太, 津田聡, 中澤徹

日本眼科学会雑誌　124　(臨増)　183-183　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
家兎濾過手術モデルでの偏光OCTの瘢痕定量評価と組織学的所見の関連性

國分太貴, 津田聡, 浅野俊文, 鈴木哲章, 前川重人, 横山悠, 佐藤孝太, 中澤徹

日本眼科学会雑誌　124　(臨増)　214-214　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
Cornea hysteresisと濾過手術成績との関連

内田惠子, 檜森紀子, 國分太貴, 二宮高洋, 志賀由己浩, 前川重人, 津田聡, 面高宗子, 横山悠, 中澤徹

日本眼科学会雑誌　124　(臨増)　214-214　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
家兎におけるカルパイン活性の生体内イメージング

浅野俊文, 津田聡, 國方彦志, 神谷真子, 浦野泰照, 中澤徹

日本眼科学会雑誌　124　(臨増)　234-234　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
開放隅角緑内障患者の前房水メタボローム解析

横山悠, 佐藤孝太, 三枝大輔, 藤岡周, 國分太貴, 前川重人, 津田聡, 面高宗子, 中澤徹

日本眼科学会雑誌　124　(臨増)　238-238　2020/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
緑内障病型別の線維柱帯切開術(眼内法)の短期術後成績

山口知暁, 横山悠, 山田晃生, 津田聡, 志賀由己浩, 前川重人, 中澤徹

日本緑内障学会抄録集　30回　81-81　2019/09
Publisher: 日本緑内障学会
隅角色素沈着量と開放隅角緑内障の線維柱帯切開術(眼内法)の短期術後成績

横山悠, 山田晃生, 津田聡, 志賀由己浩, 前川重人, 中澤徹

日本緑内障学会抄録集　30回　81-81　2019/09
Publisher: 日本緑内障学会
緑内障における房水組成と創傷治癒緑内障のバイオマーカーとしての前房水中のサイトカインとmiRNA

津田聡

日本緑内障学会抄録集　30回　112-112　2019/09
Publisher: 日本緑内障学会
緑内障におけるOCT検査の革新と進歩前眼部偏光OCTによる線維柱帯切除術後の濾過胞評価

國分太貴, 津田聡, 山成正宏, 國方彦志, 前川重人, 横山悠, 檜森紀子, 面高宗子, 中澤徹

日本緑内障学会抄録集　30回　124-124　2019/09
Publisher: 日本緑内障学会
緑内障患者における抗酸化力と角膜可塑性の関連

内田惠子, 檜森紀子, 橋本和樹, 志賀由己浩, 面高宗子, 津田聡, 中澤徹

日本緑内障学会抄録集　30回　183-183　2019/09
Publisher: 日本緑内障学会
開放隅角緑内障1家系におけるOPTN遺伝子の新規N51T変異の同定

前川重人, 橋本和軌, 志賀由己浩, 津田聡, 面高宗子, 横山悠, 西口康二, 中澤徹, 日本緑内障学会遺伝子研究班

日本緑内障学会抄録集　30回　184-184　2019/09
Publisher: 日本緑内障学会
【緑内障の早期発見・眼圧コントロール】前視野緑内障の診断方法

津田聡

眼科グラフィック　8　(4)　364-378　2019/08
Publisher: (株)メディカ出版
ISSN： 2187-2422
Flammer症候群問診スコアと寒冷負荷試験に対する血管応答の関連

高橋成奈, 志賀由己浩, 清田直樹, 安田正幸, 佐藤茉莉華, 津田聡, 面高宗子, 檜森紀子, 高山真, 石井正, 中澤徹

日本眼科学会雑誌　123　(臨増)　221-221　2019/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
高酸素血症時の血管応答性とその後の緑内障視野障害進行の関連

清田直樹, 志賀由己浩, 安田正幸, 相澤奈帆子, 面高宗子, 津田聡, 國方彦志, 中澤徹

日本眼科学会雑誌　123　(臨増)　156-156　2019/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
健常者におけるOCT-Angiographyによるradial peripapillary capillary密度マップ

面高宗子, 木川勉, 浅野俊文, 津田聡, 國方彦志, 秋葉正博, 中澤徹

日本眼科学会雑誌　123　(臨増)　221-221　2019/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
眼血流低下型緑内障の検出方法とその特徴

藤岡俊亮, 面高宗子, 安光州, 津田聡, 志賀由己浩, 木川勉, 秋葉正博, 横田秀夫, 中澤徹

日本眼科学会雑誌　122　(臨増)　212-212　2018/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
POAGとNVGにおける線維柱帯切除術後の濾過胞構造変化の比較

國分太貴, 津田聡, 相澤奈帆子, 檜森紀子, 横山悠, 面高宗子, 国松志保, 國方彦志, 中澤徹

日本眼科学会雑誌　122　(臨増)　160-160　2018/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
OCT‐Angiographyによるradial peripapillary capillary密度の緑内障診断力

津田聡, 面高宗子, 木川勉, 浅野俊文, 國方彦志, 相見太樹, 秋葉正博, 中澤徹

日本眼科学会雑誌　122　(臨増)　211-211　2018/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
【進化するOCT活用術-基礎から最新まで-】緑内障

津田聡, 中澤徹

OCULISTA　(60)　17-26　2018/03
Publisher: (株)全日本病院出版会
ISSN： 2187-5855
線維柱帯切除術施行患者における全身循環動態の検討

二宮高洋, 津田聡, 國分太貴, 志賀由己浩, 面高宗子, 檜森紀子, 横山悠, 国松志保, 中澤徹

日本緑内障学会抄録集　29th　2018
ブリモニジンまたはチモロールで24ヶ月間治療された原発開放隅角緑内障における視野障害進行関連因子の検討

横山悠, 川崎良, 高橋秀肇, 前川重人, 津田聡, 面高宗子, 中澤徹

日本緑内障学会抄録集　29th　2018
緑内障患者におけるFlammer問診票の有用性

高橋成奈, 志賀由己浩, 安田正幸, 佐藤茉莉華, 清田直樹, 津田聡, 面高宗子, 檜森紀子, 高山真, 石井正, 中澤徹

日本緑内障学会抄録集　29th　2018
緑内障患者のベースライン4分割視神経乳頭組織血流が対応する部位の視野進行に与える影響

清田直樹, 志賀由己浩, 安田正幸, 相澤奈帆子, 面高宗子, 津田聡, 檜森紀子, 國方彦志, 中澤徹

日本緑内障学会抄録集　29th　76　2018
緑内障患者におけるミトコンドリア障害マーカーとしてのmtDNA/nDNA比と視神経乳頭組織血流の検討

柳町真希, 檜森紀子, 佐藤孝太, 國分太貴, 浅野俊文, 志賀由己浩, 津田聡, 國方彦志, 中澤徹

日本緑内障学会抄録集　29th　77　2018
前視野緑内障眼での視野進行に関与する因子の検討

相澤奈帆子, 國方彦志, 志賀由己浩, 津田聡, 面高宗子, 横山悠, 国松志保, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　29th　76　2018
新規開発蛍光プローブを用いたカルパイン活性の評価

津田聡, 津田聡, 浅野俊文, 伊藤梓, 佐藤孝太, 藤田幸輔, 國方彦志, 神谷真子, 浦野泰照, 中澤徹

日本緑内障学会抄録集　29th　128　2018
新規開発蛍光プローブを用いたカルパイン活性の生体内イメージング

浅野俊文, 津田聡, 伊藤梓, 佐藤孝太, 藤田幸輔, 國方彦志, 神谷真子, 浦野泰照, 中澤徹

日本緑内障学会抄録集　29th　127　2018
New Information of Glaucoma 前視野緑内障

津田聡, 中澤徹

Frontiers in Glaucoma　(54)　156-161　2017/09
Publisher: (株)メディカルレビュー社
ISSN： 1345-854X
Classification of optic disc shape in glaucoma using machine learning based on quantified ocular parameters from ophthalmic examination instruments

117　(47)　63-66　2017/05/25
Publisher: 電子情報通信学会
ISSN： 0913-5685
前視野緑内障眼におけるプロスタグランジン関連薬による乳頭血流改善効果に関する検討

横山悠, 志賀由己浩, 相澤奈帆子, 津田聡, 清田直樹, 前川重人, 面高宗子, 米谷優輝子, 国松志保, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　121　(臨増)　281-281　2017/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
前視野緑内障眼における中心視野障害進行と視神経乳頭組織血流の関係性

相澤奈帆子, 國方彦志, 志賀由己浩, 津田聡, 面高宗子, 横山悠, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　121　(臨増)　206-206　2017/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
前眼部OCTを用いた線維柱帯切除術後の予後予測の検討

國分太貴, 津田聡, 國方彦志, 横山悠, 檜森紀子, 国松志保, 丸山和一, 中澤徹

日本眼科学会雑誌　121　(臨増)　245-245　2017/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
前視野緑内障

津田聡, 中澤徹

Frontiers in Glaucoma　(54)　2017

ISSN： 1345-854X
緑内障患者の前房水中サイトカインのプロファイルの検討

國分太貴, 津田聡, 國方彦志, 横山悠, 面高宗子, 檜森紀子, 国松志保, 丸山和一, 中澤徹

日本眼科学会雑誌　121　(1)　93-93　2017/01
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
Preperimetric glaucoma study 1: Analysis of visual field defect in preperimetric glaucoma

Naoko Aizawa, Yukihiro Shiga, Satoru Tsuda, Kazuko Omodaka, Yu Yokoyama, Tomoki Yasui, Keiichi Kato, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　57　(12)　2016/09

ISSN： 0146-0404

eISSN： 1552-5783
Imaging of scar tissue in the filtering bleb by anterior-segment polarization-sensitive OCT

Masahiro Yamanari, Satoru Tsuda, Taiki Kokubun, Kazuko Omodaka, Yu Yokoyama, Noriko Himori, Shiho Kunimatsu-Sanuki, Kazuichi Maruyama, Hiroshi Kunikata, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　57　(12)　2016/09

ISSN： 0146-0404

eISSN： 1552-5783
Preperimetric glaucoma study 2: The relationship between optic nerve head blood flow and visual field progression in preperimetric glaucoma

Yukihiro Shiga, Naoko Aizawa, Satoru Tsuda, Kazuko Omodaka, Yu Yokoyama, Tomoki Yasui, Keiichi Kato, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　57　(12)　2016/09

ISSN： 0146-0404

eISSN： 1552-5783
濾過胞観察におけるOCT減衰係数画像の有用性の検討

國分太貴, 國分太貴, 津田聡, 山成正宏, 國方彦志, 面高宗子, 横山悠, 檜森紀子, 國松志保, 丸山和一, 中澤徹

日本眼科学会雑誌　120　169　2016/03/02

ISSN： 0029-0203
極早期緑内障病態研究極早期緑内障眼での視野障害発症部位の検討

相澤奈帆子, 志賀由己浩, 津田聡, 面高宗子, 横山悠, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　120　(臨増)　163-163　2016/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
低眼灌流圧を有する正常眼圧緑内障眼の視神経乳頭血流と構造変化

米谷優輝子, 志賀由己浩, 鎌田泰彰, 面高宗子, 横山悠, 津田聡, 高橋秀肇, 中澤徹

日本眼科学会雑誌　120　(臨増)　166-166　2016/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
極早期緑内障病態研究極早期緑内障眼における視神経乳頭血流の視野進行への関与

志賀由己浩, 相澤奈帆子, 津田聡, 面高宗子, 横山悠, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　120　(臨増)　166-166　2016/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
極早期緑内障病態研究 1:極早期緑内障における視野進行と視神経乳頭血流の関係性

相澤奈帆子, 國方彦志, 志賀由己浩, 津田聡, 面高宗子, 横山悠, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　27th　162　2016
緑内障患者の前房水中サイトカインのプロファイルの検討

國分太貴, 津田聡, 國方彦志, 横山悠, 面高宗子, 檜森紀子, 国松志保, 丸山和一, 中澤徹

日本緑内障学会抄録集　27th　71　2016
In vivoイメージングによるNMDA障害後の網膜神経節細胞死の評価

伊藤梓, 國方彦志, 前川重人, 津田聡, 佐藤孝太, 浅井信晴, 中澤徹

日本緑内障学会抄録集　27th　89　2016
パスウェイ特異的プロモーターを用いた網膜神経節細胞ストレス応答のin vivoイメージング

藤田幸輔, 西口康二, 横山悠, 富山優介, 津田聡, 安田正幸, 中澤徹

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　88回・38回　[4T18p-11(3P1293)]　2015/12
Publisher: (公社)日本生化学会
極早期緑内障患者に対するタフルプロスト点眼液による治療成績と視野障害発症症例の患者背景

相澤奈帆子, 國方彦志, 志賀由己浩, 津田聡, 面高宗子, 横山悠, 劉孟林, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　26回　123-123　2015/09
Publisher: 日本緑内障学会
Effect of a PG analogue on optic nerve head blood flow in eyes with preperimetric glaucoma

Naoki Kiyota, Yukihiro Shiga, Naoko Aizawa, Satoru Tsuda, Kazuko Omodaka, Tomoki Yasui, Keiichi Kato, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　56　(7)　2015/06

ISSN： 0146-0404

eISSN： 1552-5783
Preperimetric glaucoma pathophysiological study part1:Twelve-month results of topical prostaglandin analogues

Naoko Aizawa, Hiroshi Kunikata, Satoru Tsuda, Yukihiro Shiga, Yukiko Maiya, Naoki Kiyota, Kazuko Omodaka, Yu Yokoyama, Morin Ryu, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　56　(7)　2015/06

ISSN： 0146-0404

eISSN： 1552-5783
Preperimetric glaucoma pathophysiological study part4: Significant reduction in optic nerve head blood flow

Yukiko Maiya, Yukihiro Shiga, Naoko Aizawa, Satoru Tsuda, Yu Yokoyama, Kazuko Omodaka, Tomoki Yasui, Keiichi Kato, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　56　(7)　2015/06

ISSN： 0146-0404

eISSN： 1552-5783
Preperimetric glaucoma pathophysiological study part3: Association between EDNRA genotype and the effect of a prostaglandin analogue on ocular blood flow

Yukihiro Shiga, Nobuo Fuse, Kota Sato, Naoko Aizawa, Satoru Tsuda, Masae Kimura, Koji Nishiguchi, Tomoki Yasui, Keiichi Kato, Toru Nakazawa

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　56　(7)　2015/06

ISSN： 0146-0404

eISSN： 1552-5783
マウス視神経挫滅モデルにおけるScotopic Threshold Response早期変化

雪田昌克, 町田繁樹, 西口康二, 津田聡, 田中佑治, 横山悠, 安田正幸, 丸山和一, 中澤徹

眼科臨床紀要　8　(4)　251-251　2015/04
Publisher: 眼科臨床紀要会
ISSN： 1882-5176
極早期緑内障病態研究プラスタグランジン関連薬の乳頭血流改善効果に関する検討

清田直樹, 志賀由己浩, 相澤奈帆子, 津田聡, 横山悠, 面高宗子, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　119　(臨増)　231-231　2015/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
極早期緑内障病態研究極早期緑内障眼における視神経乳頭血流動態に関する検討

米谷優輝子, 志賀由己浩, 相澤奈帆子, 津田聡, 横山悠, 面高宗子, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　119　(臨増)　235-235　2015/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
極早期緑内障病態研究 1:プロスタグランジン関連薬による12カ月長期治療成績

相澤奈帆子, 國方彦志, 津田聡, 志賀由己浩, 清田直樹, 米谷優輝子, 面高宗子, 横山悠, 劉孟林, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　119　(臨増)　230-230　2015/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
パスウェイ特異的レポーターによる網膜神経節細胞ストレス応答のin vivoイメージング

西口康二, 藤田幸輔, 藤井裕介, 山本耕太郎, 津田聡, 安田正幸, 中澤徹

日本眼科学会雑誌　119　(臨増)　244-244　2015/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
マウス視神経挫滅モデルにおけるscotopic threshold response早期変化

雪田昌克, 町田繁樹, 西口康二, 津田聡, 横山悠, 安田正幸, 丸山和一, 中澤徹

日本眼科学会雑誌　119　(2)　80-80　2015/02
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
前眼部偏光OCTによる濾過胞瘢痕組織の複屈折イメージングと定量解析

山成正宏, 津田聡, 國分太貴, 檜森紀子, 國松志保, 高橋秀肇, 丸山和一, 國方彦志, 中澤徹

日本眼光学学会総会プログラム・抄録集　51st　61　2015
極早期緑内障病態研究研究デザインと患者背景

劉孟林, 横山悠, 相澤奈帆子, 面高宗子, 津田聡, 志賀由己浩, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　25回　86-86　2014/09
Publisher: 日本緑内障学会
極早期緑内障病態研究極早期緑内障患者における乳頭内血管面積の動態

清田直樹, 志賀由己浩, 津田聡, 相澤奈帆子, 横山悠, 面高宗子, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　25回　87-87　2014/09
Publisher: 日本緑内障学会
極早期緑内障病態研究極早期緑内障眼における遺伝子多型とプロスタグランジン関連薬による乳頭循環反応

志賀由己浩, 布施昇男, 清水愛, 木村雅恵, 津田聡, 相澤奈帆子, 横山悠, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　25回　87-87　2014/09
Publisher: 日本緑内障学会
局所性障害に対する乳頭形状別の血流関与についての検討

米谷優輝子, 高橋秀肇, 志賀由己浩, 面高宗子, 津田聡, 横山悠, 国松志保, 中澤徹

日本緑内障学会抄録集　25回　87-87　2014/09
Publisher: 日本緑内障学会
正常眼圧緑内障患者における神経走行を考慮したOCT黄斑セクターの設定

井上真希, 面高宗子, 横山悠, 志賀由己浩, 津田聡, 檜森紀子, 丸山和一, 中澤徹

日本緑内障学会抄録集　25回　101-101　2014/09
Publisher: 日本緑内障学会
視野進行における局所性障害の評価の検討

高橋秀肇, 志賀由己浩, 面高宗子, 津田聡, 横山悠, 国松志保, 中澤徹

日本緑内障学会抄録集　25回　105-105　2014/09
Publisher: 日本緑内障学会
極早期緑内障病態研究 2:極早期緑内障患者における視神経乳頭血流の薬剤反応性

相澤奈帆子, 國方彦志, 津田聡, 志賀由己浩, 清田直樹, 面高宗子, 横山悠, 劉孟林, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　25回　86-86　2014/09
Publisher: 日本緑内障学会
In vivo imagingによる視神経挫滅後の網膜神経節細胞死の評価

津田聡, 田中佑治, 國方彦志, 横山悠, 安田正幸, 中澤徹

日本緑内障学会抄録集　25回　115-115　2014/09
Publisher: 日本緑内障学会
【先端的眼科治療(角膜と網膜の新しい治療法)】緑内障における神経保護治療

津田聡, 中澤徹

Medical Science Digest　40　(3)　120-123　2014/03
Publisher: (株)ニュー・サイエンス社
ISSN： 1347-4340
Registration Algorithm of Fundus Oculi for 3-D Optical Coherence Tomography Images

TOGASHI Kyosuke, SAKAMOTO Miki, TAKEUCHI Gaku, AKIBA Masahiro, YABANA Takeshi, OMODAKA Kazuko, RYU Morin, TSUDA Satoru, YOKOYAMA Yu, FUKUMA Yasufumi, NAKAZAWA Toru, YUASA Tetsuya

IEICE technical report.　113　(410)　133-137　2014/01/26
Publisher: The Institute of Electronics, Information and Communication Engineers
ISSN： 0913-5685

More details Close

Recently, retinal nerve fiber layer (RNFL) thickness measurement from retinal optical coherence tomography (OCT) images has been widely used as an important clinical index to indicate a glaucoma progression. While a long-term follow-up of RNFL thickness is effective tool for glaucoma diagnosis, a precise registration is indispensable for OCT images acquired at different date. In this study, we propose a registration algorithm to correct random shifts occurring whenever imaging based on a projection image generated from the 3-D OCT retinal images. For speeding-up, the algorithm was implemented by processing in Fourier space based on properties of Fourier transform with rotation and translation, which is referred to as phase-only correlation method. For the stable detection of shift, several preprocessing to raw data, such as background correction to homogenize different imaging conditions, zero-padding to resize the registration image from a rectangular to a square image size, and Hanning-windowing to suppress unnecessary higher frequency-components, were carefully applied before registration. We demonstrated the effectiveness of the proposed method using an actual retina image (512×885×64) of a healthy volunteer acquired by 3-D OCT-2000 manufactured by TOPCON, Japan. The measurement was repeated 10 times, and the degree of agreement in RNFL thickness measured was evaluated with root mean square (RMS). The average RMS before and after preregistration were 4.7±1.6 and 2.6±0.3 μm, respectively. From this result, it can be concluded that the proposed method improved the precision approximately twofold. In addition, the processing time was approximately 0.3 s for a single data set, which will fall inside a permissible range of clinical use. Proposed technique may become a useful tool for glaucoma diagnosis.
前眼部偏光光干渉断層計による濾過胞内の線維化組織の観察

國分太貴, 津田聡, 山成正宏, 田中佑治, 志賀由己浩, 横山悠, 国松志保, 高橋秀肇, 丸山和一, 國方彦志, 中澤徹

日本緑内障学会抄録集　25th　90　2014
濾過胞の生存に関与する前房水サイトカインの検討

國分太貴, 津田聡, 安田正幸, 清水愛, 横山悠, 渡邊亮, 劉孟林, 国松志保, 丸山和一, 國方彦志, 中澤徹

日本眼科学会雑誌　118　(1)　48-48　2014/01
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
緑内障進行におけるLSFG乳頭波形解析の検討

高橋麻衣, 高橋秀肇, 志賀由己浩, 面高宗子, 今野瑛之, 津田聡, 横山悠, 国松志保, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　58-58　2013/09
Publisher: 日本緑内障学会
ステレオ眼底カメラにより測定した視神経乳頭傾斜角度と他のパラメーターの相関

横山悠, 谷戸正樹, 新田耕治, 片井麻貴, 北岡康史, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　61-61　2013/09
Publisher: 日本緑内障学会
SS-OCTによるBMO手動解析スキャンパターン間の差異と再現性の検討

高橋世理, 津田聡, 面高宗子, 鎌田泰彰, 横山悠, 中澤徹

日本緑内障学会抄録集　24回　62-62　2013/09
Publisher: 日本緑内障学会
緑内障患者の乳頭形状分類を用いた乳頭周囲網膜神経線維層厚の障害部位の検討

高田菜生子, 面高宗子, 志賀由己浩, 津田聡, 横山悠, 檜森紀子, 劉孟林, 高橋秀肇, 中澤徹

日本緑内障学会抄録集　24回　65-65　2013/09
Publisher: 日本緑内障学会
緑内障半視野進行症例におけるMDスロープ、半視野TDスロープ、OCTベース視野セクターTDスロープの比較

今野瑛之, 国松志保, 横山悠, 面高宗子, 津田聡, 高橋秀肇, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　111-111　2013/09
Publisher: 日本緑内障学会
極早期緑内障患者のプロスタグランジン関連薬による乳頭循環反応性に関与する因子

清田直樹, 志賀由己浩, 津田聡, 相澤奈帆子, 横山悠, 面高宗子, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　24回　114-114　2013/09
Publisher: 日本緑内障学会
極早期緑内障患者における視神経乳頭血流波形に関する検討

志賀由己浩, 面高宗子, 清田直樹, 津田聡, 相澤奈帆子, 横山悠, 安井朝輝, 加藤圭一, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　114-114　2013/09
Publisher: 日本緑内障学会
緑内障進行における血流関与についての検討

高橋秀肇, 高橋麻衣, 今野瑛之, 志賀由己浩, 面高宗子, 津田聡, 横山悠, 国松志保, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　114-114　2013/09
Publisher: 日本緑内障学会
東北大学病院における毛様体光凝固術の術後成績

多田麻子, 国松志保, 丸山和一, 高橋秀肇, 劉孟林, 渡邉亮, 檜森紀子, 横山悠, 津田聡, 志賀由己浩, 中澤徹

日本緑内障学会抄録集　24回　152-152　2013/09
Publisher: 日本緑内障学会
極早期緑内障患者における視神経乳頭血流の薬剤反応性

相澤奈帆子, 國方彦志, 津田聡, 志賀由己浩, 面高宗子, 横山悠, 劉孟林, 安井朝輝, 加藤圭一, 中澤徹

日本緑内障学会抄録集　24回　115-115　2013/09
Publisher: 日本緑内障学会
濾過胞の生存に関与する前房水サイトカインの検討

國分太貴, 津田聡, 安田正幸, 清水愛, 横山悠, 渡邉亮, 劉孟林, 国松志保, 丸山和一, 中澤徹

日本緑内障学会抄録集　24回　47-47　2013/09
Publisher: 日本緑内障学会
緑内障に関連する前房水miRNAマーカーのマイクロアレイ解析

津田聡, 田中佑治, 安田正幸, 佐藤順子, 國分太貴, 中澤徹

日本緑内障学会抄録集　24回　51-51　2013/09
Publisher: 日本緑内障学会
ステレオ眼底カメラにより測定した視神経乳頭リム偏心率と他のパラメーターの相関

片井麻貴, 谷戸正樹, 新田耕治, 北岡康史, 横山悠, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　24回　60-60　2013/09
Publisher: 日本緑内障学会
緑内障患者における病期別、年齢別の機能と構造の相関

萱場寛子, 横山悠, 多田麻子, 津田聡, 面高宗子, 國松志保, 丸山和一, 中澤徹

日本眼科学会雑誌　117　(臨増)　334-334　2013/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
前眼部3次元OCTを用いた線維柱帯切除術後の濾過胞の評価

國分太貴, 津田聡, 浅野俊文, 横山悠, 面高宗子, 多田麻子, 渡邉亮, 劉孟林, 國松志保, 高橋秀肇, 丸山和一, 中澤徹

日本眼科学会雑誌　117　(臨増)　338-338　2013/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
線維柱帯切除後の濾過胞における経時的結膜血管面積の定量化

浅野俊文, 津田聡, 國分太貴, 横山悠, 面高宗子, 渡邉亮, 劉孟林, 国松志保, 高橋秀肇, 丸山和一, 中澤徹

日本眼科学会雑誌　117　(臨増)　340-340　2013/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
低酸素応答生理活性蛍光プローブを用いた網膜低酸素領域のin vivoイメージング

津田聡, 田中佑治, 朴文, 國方彦志, 横山悠, 安田正幸, 花岡健二郎, 長野哲雄, 中澤徹

日本眼科学会雑誌　117　(臨増)　280-280　2013/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
エリスロポエチンノックアウトマウスを用いた網膜・視神経の組織学的検討

安田正幸, 劉孟林, 田中佑治, 檜森紀子, 面高宗子, 横山悠, 雪田昌克, 津田聡, 丸山和一, 山本雅之, 中澤徹

日本眼科学会雑誌　117　(臨増)　311-311　2013/03
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
緑内障における神経保護治療

津田聡, 中澤徹

Medical Science Digest　40　(3)　14-17　2013
【生活習慣(病)と網膜関連疾患の関与を探る】緑内障

津田聡, 中澤徹

Retina Medicine　1　(1)　47-52　2012/10
Publisher: (株)先端医学社
ISSN： 2187-2384
レーザースペックルフローグラフィを用いた乳頭内血管面積の緑内障診断における有用性

前川重人, 志賀由己浩, 面高宗子, 津田聡, 横山悠, 渡邊亮, 劉孟林, 国松志保, 高橋秀肇, 中澤徹

日本緑内障学会抄録集　23回　98-98　2012/09
Publisher: 日本緑内障学会
緑内障評価におけるLSFG-NAVIの新しい波形解析パラメータFlow Acceleration Indexの有用性

今野瑛之, 横山悠, 志賀由己浩, 面高宗子, 津田聡, 渡邊亮, 劉孟林, 国松志保, 高橋秀肇, 中澤徹

日本緑内障学会抄録集　23回　98-98　2012/09
Publisher: 日本緑内障学会
ステレオ眼底カメラによる視神経乳頭形状解析に関する多施設共同研究(GSAS)の乳頭形状パラメーター

横山悠, 谷戸正樹, 新田耕治, 片井麻貴, 北岡康史, 面高宗子, 津田聡, 中川俊明, 中澤徹

日本緑内障学会抄録集　23回　110-110　2012/09
Publisher: 日本緑内障学会
局所虚血型緑内障における乳頭周囲網膜神経線維層厚菲薄化の血流閾値

津田聡, 横山悠, 國分太貴, 面高宗子, 劉孟林, 国松志保, 高橋秀肇, 中澤徹

日本緑内障学会抄録集　23回　154-154　2012/09
Publisher: 日本緑内障学会
緑内障眼における視神経乳頭循環と脈絡膜循環の血流波形の差異

國分太貴, 津田聡, 志賀由己浩, 横山悠, 面高宗子, 前川重人, 渡邊亮, 劉孟林, 国松志保, 高橋秀肇, 中澤徹

日本緑内障学会抄録集　23回　159-159　2012/09
Publisher: 日本緑内障学会
25G硝子体手術中に発見された硝子体腔中の遊離浮遊嚢腫

志賀由己浩, 國方彦志, 渡辺みか, 津田聡, 中澤徹

眼科手術　25　72　2011/12/28

ISSN： 0914-6806
レーザースペックルフローグラフィを用いた体位変換時の眼底血流評価

志賀由己浩, 中澤徹, 横山悠, 相澤菜帆子, 千葉真生, 津田聡

日本眼科学会雑誌　115　(臨増)　200-200　2011/04
Publisher: (公財)日本眼科学会
ISSN： 0029-0203
タフルプロスト点眼による視神経乳頭循環への影響

津田聡, 中澤徹, 千葉真生, 相澤奈緒子, 横山悠, 小野省太, 布施昇男

日本緑内障学会抄録集　21回　108-108　2010/09
Publisher: 日本緑内障学会

Show all ︎Show first 5

Presentations 35

眼血流低下型緑内障の検出方法とその特徴

藤岡俊亮, 面高宗子, 安光州, 津田聡, 志賀由己浩, 木川勉, 秋葉正博, 横田秀夫, 中澤徹

日本眼科学会雑誌　2018/03
OCT-AngiographyによるRadial peripapillary capillary密度の緑内障診断力

津田聡, 面高宗子, 木川勉, 浅野俊文, 國方彦志, 相見太樹, 秋葉正博, 中澤徹

日本眼科学会雑誌　2018/03
POAGとNVGにおける線維柱帯切除術後の濾過胞構造変化の比較

國分太貴, 津田聡, 相澤奈帆子, 檜森紀子, 横山悠, 面高宗子, 国松志保, 國方彦志, 中澤徹

日本眼科学会雑誌　2018/03
前視野緑内障眼におけるプロスタグランジン関連薬による乳頭血流改善効果に関する検討

横山悠, 志賀由己浩, 相澤奈帆子, 津田聡, 清田直樹, 前川重人, 面高宗子, 米谷優輝子, 国松志保, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　2017/03
前眼部OCTを用いた線維柱帯切除術後の予後予測の検討

國分太貴, 津田聡, 國方彦志, 横山悠, 檜森紀子, 国松志保, 丸山和一, 中澤徹

日本眼科学会雑誌　2017/03
前視野緑内障眼における中心視野障害進行と視神経乳頭組織血流の関係性

相澤奈帆子, 國方彦志, 志賀由己浩, 津田聡, 面高宗子, 横山悠, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　2017/03
緑内障患者の前房水中サイトカインのプロファイルの検討

國分太貴, 津田聡, 國方彦志, 横山悠, 面高宗子, 檜森紀子, 国松志保, 丸山和一, 中澤徹

日本眼科学会雑誌　2017/01
濾過胞観察におけるOCT減衰係数画像の有用性の検討

國分太貴, 津田聡, 山成正宏, 國方彦志, 面高宗子, 横山悠, 檜森紀子, 國松志保, 丸山和一, 中澤徹

日本眼科学会雑誌　2016/03
極早期緑内障病態研究極早期緑内障眼における視神経乳頭血流の視野進行への関与

志賀由己浩, 相澤奈帆子, 津田聡, 面高宗子, 横山悠, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　2016/03
低眼灌流圧を有する正常眼圧緑内障眼の視神経乳頭血流と構造変化

米谷優輝子, 志賀由己浩, 鎌田泰彰, 面高宗子, 横山悠, 津田聡, 高橋秀肇, 中澤徹

日本眼科学会雑誌　2016/03
極早期緑内障病態研究極早期緑内障眼での視野障害発症部位の検討

相澤奈帆子, 志賀由己浩, 津田聡, 面高宗子, 横山悠, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　2016/03
マウス視神経挫滅モデルにおけるScotopic Threshold Response早期変化

雪田昌克, 町田繁樹, 西口康二, 津田聡, 田中佑治, 横山悠, 安田正幸, 丸山和一, 中澤徹

眼科臨床紀要　2015/04
パスウェイ特異的レポーターによる網膜神経節細胞ストレス応答のin vivoイメージング

西口康二, 藤田幸輔, 藤井裕介, 山本耕太郎, 津田聡, 安田正幸, 中澤徹

日本眼科学会雑誌　2015/03
極早期緑内障病態研究極早期緑内障眼における視神経乳頭血流動態に関する検討

米谷優輝子, 志賀由己浩, 相澤奈帆子, 津田聡, 横山悠, 面高宗子, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　2015/03
極早期緑内障病態研究プラスタグランジン関連薬の乳頭血流改善効果に関する検討

清田直樹, 志賀由己浩, 相澤奈帆子, 津田聡, 横山悠, 面高宗子, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　2015/03
極早期緑内障病態研究プロスタグランジン関連薬による12ヵ月長期治療成績

相澤奈帆子, 國方彦志, 津田聡, 志賀由己浩, 清田直樹, 米谷優輝子, 面高宗子, 横山悠, 劉孟林, 安井朝輝, 加藤圭一, 中澤徹

日本眼科学会雑誌　2015/03
マウス視神経挫滅モデルにおけるscotopic threshold response早期変化

雪田昌克, 町田繁樹, 西口康二, 津田聡, 横山悠, 安田正幸, 丸山和一, 中澤徹

日本眼科学会雑誌　2015/02
局所性障害に対する乳頭形状別の血流関与についての検討

米谷優輝子, 高橋秀肇, 志賀由己浩, 面高宗子, 津田聡, 横山悠, 国松志保, 中澤徹

日本緑内障学会抄録集　2014/09
濾過胞の生存に関与する前房水サイトカインの検討

國分太貴, 津田聡, 安田正幸, 清水愛, 横山悠, 渡邊亮, 劉孟林, 国松志保, 丸山和一, 國方彦志, 中澤徹

日本眼科学会雑誌　2014/01
極早期緑内障病態研究に関する研究デザイン

川上綾子, 加藤圭一, 横山悠, 相澤奈帆子, 面高宗子, 津田聡, 志賀由己浩, 安井朝輝, 中澤徹

日本緑内障学会抄録集　2013/09
東北大学病院における毛様体光凝固術の術後成績

多田麻子, 国松志保, 丸山和一, 高橋秀肇, 劉孟林, 渡邉亮, 檜森紀子, 横山悠, 津田聡, 志賀由己浩, 中澤徹

日本緑内障学会抄録集　2013/09
ステレオ眼底カメラにより測定した視神経乳頭傾斜角度と他のパラメーターの相関

横山悠, 谷戸正樹, 新田耕治, 片井麻貴, 北岡康史, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　2013/09
ステレオ眼底カメラにより計測した視神経乳頭形状パラメーターによる乳頭形状4群分類

新田耕治, 谷戸正樹, 片井麻貴, 北岡康史, 横山悠, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　2013/09
ステレオ眼底カメラにより計測した視神経乳頭形状パラメーターを用いた乳頭形状新分類

谷戸正樹, 新田耕治, 片井麻貴, 北岡康史, 横山悠, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　2013/09
ステレオ眼底カメラによる緑内障性視神経乳頭障害指標(DDLS)の算出

北岡康史, 谷戸正樹, 新田耕治, 片井麻貴, 横山悠, 面高宗子, 津田聡, 中川俊明, 内藤知子, 山下高明, 溝上志朗, 岩瀬愛子, 中澤徹

日本緑内障学会抄録集　2013/09
線維柱帯切除後の濾過胞における経時的結膜血管面積の定量化

浅野俊文, 津田聡, 國分太貴, 横山悠, 面高宗子, 渡邉亮, 劉孟林, 国松志保, 高橋秀肇, 丸山和一, 中澤徹

日本眼科学会雑誌　2013/03
前眼部3次元OCTを用いた線維柱帯切除術後の濾過胞の評価

國分太貴, 津田聡, 浅野俊文, 横山悠, 面高宗子, 多田麻子, 渡邉亮, 劉孟林, 國松志保, 高橋秀肇, 丸山和一, 中澤徹

日本眼科学会雑誌　2013/03
緑内障患者における病期別、年齢別の機能と構造の相関

萱場寛子, 横山悠, 多田麻子, 津田聡, 面高宗子, 國松志保, 丸山和一, 中澤徹

日本眼科学会雑誌　2013/03
エリスロポエチンノックアウトマウスを用いた網膜・視神経の組織学的検討

安田正幸, 劉孟林, 田中佑治, 檜森紀子, 面高宗子, 横山悠, 雪田昌克, 津田聡, 丸山和一, 山本雅之, 中澤徹

日本眼科学会雑誌　2013/03
OCTによる12分割乳頭周囲網膜神経線維層厚に対応する視野検査点の検討

今野瑛之, 国松志保, 横山悠, 面高宗子, 津田聡, 高橋麻衣, 浅野俊文, 渡邊亮, 劉孟林, 高橋秀肇, 丸山和一, 岩瀬愛子, 中澤徹

日本眼科学会雑誌　2013/03
低酸素応答生理活性蛍光プローブを用いた網膜低酸素領域のin vivoイメージング

津田聡, 田中佑治, 朴文, 國方彦志, 横山悠, 安田正幸, 花岡健二郎, 長野哲雄, 中澤徹

日本眼科学会雑誌　2013/03
後期緑内障患者における視野進行評価法

渡邉亮, 国松志保, 横山悠, 今野瑛之, 浅野俊文, 津田聡, 面高宗子, 劉孟林, 高橋秀肇, 中澤徹

日本眼科学会雑誌　2013/03
ステレオ眼底カメラにより計測した視神経乳頭形状パラメーターの乳頭形状4分類間比較

新田耕治, 谷戸正樹, 片井麻貴, 北岡康史, 横山悠, 面高宗子, 津田聡, 中川俊明, 中澤徹

日本緑内障学会抄録集　2012/09
ステレオ眼底カメラによる視神経乳頭形状解析に関する多施設共同研究(GSAS)の乳頭形状パラメーター

横山悠, 谷戸正樹, 新田耕治, 片井麻貴, 北岡康史, 面高宗子, 津田聡, 中川俊明, 中澤徹

日本緑内障学会抄録集　2012/09
レーザースペックルフローグラフィを用いた体位変換時の眼底血流評価

志賀由己浩, 中澤徹, 横山悠, 相澤菜帆子, 千葉真生, 津田聡

日本眼科学会雑誌　2011/04

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Research Projects 6

ヒト緑内障GWASからの個別化創薬プラットフォーム構築研究

中澤徹, 石川誠, 佐藤孝太, 杉江淳, 津田聡, 大石路子

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2024/04/01 - 2027/03/31
瘢痕化定量技術と薬剤ナノ粒子化による組織移行性の高い濾過胞瘢痕抑制薬の開発

津田聡, 中澤徹, 笠井均, 小関良卓, 佐藤孝太, 國分太貴

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2021/04/01 - 2024/03/31
前眼部組織間のトランスオミクス解析による包括的な眼圧上昇機序の解明

横山悠, 中澤徹, 國分太貴, 矢花武史, 佐藤孝太, 津田聡, 前川重人, 三枝大輔

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2021/04/01 - 2024/03/31

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本研究は緑内障病態理解のため、眼科的臨床データと前房水と線維柱帯のメタボローム解析、プロテオーム解析等を用いた包括的な関連解析を行う研究である。本年度はおもに線維柱帯切開術を行うことを予定された広義原発開放隅角緑内障を対象にサンプル採取を行う計画であった。患者に研究計画を説明し同意を書面上で取得した。手術のための末梢ライン確保時に採血、また同日に検尿を行った上でKahook dual bladeを用いた線維柱帯採取を試みた。しかし、Kahook Dual bladeは新しい型へと変更しており、よりBlade部が小さく改良された。そのため検体採取が手技的に難しくなり、何例がで採取を試みたが検体を得られなかった。現在は旧タイプのKahook Dual bladeの入手は困難となったため、25ゲージ硝子体手術用鑷子、さらにはまだ試用中であったTrabEx+(TM)を用いた線維柱帯採取を試みたが採取部位が非常に小さいこと、出血による視認困難などの理由により。採取できなかった。ほかには代替案として濾過手術など別術式による採取を検討している。濾過手術は虹彩も切除するためより包括的な前房内環境の解析が可能となる。しかし術中、マイトマイシンCなどの薬剤を使用するために解析に何らかの影響を及ぼすことが否定できない。引き続き線維柱帯切開術時、ケリー氏トラベクレクトミーパンチ等別の硝子体手術鑷子等の眼科手術器具を手配して検体採取を行う予定である。
Establishment of novel biomarkers and drug discovery bases for the development of filter bleb scarring inhibitors

Satoru Tsuda

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Early-Career Scientists

Institution: Tohoku University

2019/04/01 - 2021/03/31

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For improvement of the surgical results of glaucoma, the scarring of the filtration bleb after trabeculectomy in the rabbit is non-invasively quantified as a birefringence value with the anterior segment polarization sensitive OCT, which is a medical device originated from Japan. A strong relationship was found between the birefringence value and the collagen density of the tissue section. In addition, in rabbit trabeculectomy using a known scar suppressant, the intraocular pressure lowering effect continued for a long period of time, the birefringence value due to anterior segment PS-OCT was low after surgery, and collagen density in the excised tissue section was decreasing. Based on the above, we have established a biomarker that can quantitatively evaluate the scarring of the filtration bleb by the anterior segment PS-OCT, and clarified that it can be used to evaluate the efficacy of scar suppressants.
活性イオウ分子種を用いた新規抗酸化眼内灌流液の開発

Offer Organization: 日本医療研究開発機構

System: 革新的医療技術創出拠点プロジェクト

Institution: 東北大学

2020/04 - 2021/03
Role of transcription factor Sp1 in retinal ganglion cell death in mouse glaucoma model animals Competitive

Tsuda Satoru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

Category: Grant-in-Aid for Young Scientists (B)

Institution: Tohoku University

2017/04/01 - 2019/03/31

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When Ecel1 expression was evaluated in the mouse retina after optic nerve crush, focusing on Ecel1 downstream of Sp-1, Ecel1 expression was specifically induced in retinal ganglion cells. In addition, knocking down the Ecel1 gene using the Adeno-Associated Virus-CRISPR / Cas9 system and evaluating retinal ganglion cell survival with retrograde labeling, qRT-PCR, and visual evoked potentials, knockdown of Ecel1 promoted the loss of retinal ganglion cells after optic nerve crush. This results indicate that Ecel1 may be a novel therapeutic target for alleviation of retinal ganglion cell injury. And it is speculated that Ecel1 is involved in a part of cytoprotective mechanism of Sp-1.

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