Details of the Researcher

PHOTO

Takuji Kawamura
Section
Institute of Development, Aging and Cancer
Job title
Assistant Professor
Degree
e-Rad No.
80823327

Research History 6

  • 2026/04 - Present
    Tohoku University　Smart-Aging Research Center, Institute of Development, Aging and Cancer　Assistant Professor

  • 2024/09 - 2026/03
    Tohoku University　SmartAging Research Center　Assistant Professor

  • 2024/05 - 2024/08
    Waseda University　Faculty of Sport Sciences　Junior Researcher (Assistant Professor)

  • 2021/07 - 2024/03
    Hungarian University of Sports Science　Research Centre for Molecular Exercise Science　Postdoctoral Fellow

  • 2021/04 - 2024/03
    Waseda University　Institute for Sport Sciences　Invited Researcher

  • 2018/04 - 2021/03
    Waseda University　Faculty of Sport Sciences　Assistant Professor

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Professional Memberships 2

  • European College of Sport Science

  • THE JAPANESE SOCIETY OF PHYSICAL FITNESS AND SPORTS MEDICINE

Research Interests 4

  • Cardiorespiratory fitness

  • Geroscience

  • Epigentics

  • aging

Research Areas 2

  • Life sciences / Sports science / Geroscience

  • Life sciences / Sports science /

Awards 8

  1. 2026 GeroScience Outstanding Publication Award Honorable Mentions

    2026/07　The American Aging Association　Epigenetic insights of Olympic champions: nuclear and mitochondrial DNA methylation and regulators of aging

  2. APS Select

    2026/04　American Physiological Society　Epigenetic signatures and genetic variants associated with muscle strength in postmenopausal women: potential bone muscle cross talk via BMP1 mechanisms

  3. Early-Career Researcher Award (International Academic Exchange)

    2025/09　Japanese Society of Physical Fitness and Sports Medicine　Understanding and Predicting Mental Health in Older Adults: A Large-Scale Bayesian Neural Network Analysis of Physical Activity and Social Skills

  4. 2024 GeroScience Outstanding Publication Award Honorable Mentions

    2025/07　The American Aging Association　DNA methylation clock DNAmFitAge shows regular exercise is associated with slower aging and systemic adaptation

  5. Most Popular DOIs of 2024

    2025/01　Aging (Albany NY)　Physical fitness and lifestyles associated with biological aging

  6. 早稲田大学スポーツ科学学術院20周年記念式典・卒業生表彰

    2023/10

  7. Hungarian state scholarships for short-term or long-term study visits

    2021/09　Tempus Public Foundation

  8. 特に優れた業績による奨学金返還免除

    2015/06　独立行政法人日本学生支援機構

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Papers 44

  1. Constructing a Bayesian Neural Network Model for the Early Prediction of Psychological Distress among Older Adults: Comprehensive Analysis of Humor Styles, Communication Skills, and Social Physical Activity.

    Soga K., Kamijo K., Kawamura T., Uno A., Taki Y.

    Frontiers in Research Metrics and Analytics　11　1882045　2026/08

  2. High intrinsic aerobic capacity is associated with a distinct epigenetic and signaling profile in the aged rat brain. Peer-reviewed

    Zhou L., Mozaffaritabar S., Koltai E., Kawamura T., Higuchi M., Kujach S., Gu Y., Koch L., Britton S., Radák Z.

    Aging Cell　25　(7)　e70622　2026/07

    DOI： 10.1111/acel.70622 　

  3. Methylome–transcriptome integration reveals molecular signatures of intrinsic aerobic capacity in aged skeletal muscle. Peer-reviewed

    Zhou L., Mozaffaritabar S., Torma F., Koltai E., Kawamura T., Higuchi M., Zhong Z., Koch LG, Britton SL, Radak Z.

    Journal of Sport and Health Science (In press)　2026/06

  4. Intrinsic Exercise Capacity Is Associated with Skeletal Muscle Clock Gene and IGF-1 Signaling in Aged Low-and High-Running Capacity Rats. Peer-reviewed

    Kim HK, Kawamura T., Bori Z., Koch L., Britton S., Radák Z.

    Frontiers in Physiology　17　1818866　2026/06

  5. Preliminary Effects of a Four-Month Circuit Training Intervention on Cognitive Function and Exploratory Plasma Proteomic Profiles in Middle-Aged and Older Women: An Open-Label Randomized Controlled Trial Peer-reviewed

    Soga K., Takahashi M., Oba K., Uno A., Kawamura T., Kawashima K., Shinada T., Taki Y.

    Frontiers in Sports and Active Living　8　1851134　2026/06

    DOI： 10.3389/fspor.2026.1851134 　

  6. Epigenetic signatures and genetic variants associated with functional strength in postmenopausal women: a potential link to bone-muscle crosstalk involving BMP1 Peer-reviewed

    da Silva Rodrigues G., Noronha NY, Benjamim J., Sae-Lee C., Chitta P., da Silva Sobrinho AC, Kawamura T., Barbosa Júnior F., Nonino CB, Watanabe LM, Bueno Júnior CR

    Physiological Genomics　58　152-169　2026/03

    DOI： 10.1152/physiolgenomics.00254.2025 　

  7. Dietary regulation of epigenetic aging: Beyond clocks to gene-specific mechanisms Invited Peer-reviewed

    Kawamura T., da Silva Rodrigues G., Bueno Júnior CR, Taki Y., Higuchi M.

    The Journal of Nutritional Biochemistry　110156　2026/02

    DOI： 10.1016/j.jnutbio.2025.110156 　

  8. Social physical activity and sedentary behavior as key determinants of humor expression and loneliness in older adults: A cross-sectional study using bayesian variable selection approach. Peer-reviewed

    Soga K., Uno A., Kawamura T., Kamijo K., Taki Y.

    BMC Public Health　26　(1)　866　2026/02

    DOI： 10.1186/s12889-026-26232-8. 　

  9. Epigenetic insights of Olympic champions: nuclear and mitochondrial DNA methylation and regulators of aging Peer-reviewed

    Teglas T., Torma F., Bori Z., Aczel D., Babszky G., Kawamura T., Higuchi M., Yaodong G., Lee M., Horvath S., Radak Z.

    GeroScience (Online ahead of print)　2026/01

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s11357-025-02092-9 　

    eISSN： 2509-2723

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    Abstract The interaction between nuclear (nDNA) and mitochondrial DNA (mtDNA) methylation is not well known in the healthy population. The D-loop methylation level of the Olympic champions ( N  = 58) was significantly lower than that of non-champions ( N  = 32) (~ 36% unadjusted mean difference p  = 0.016, sex and age adjusted p  = 0.017). Interestingly, the robust linear analysis revealed that biological sex is a significant factor in mtDNA D-loop methylation (estimate = 1.521, p  = 0.033). On the other hand, we cannot find relationships between the methylation levels of mtDNA and nuclear DNA, suggesting distinct regulation of the methylation/demethylation process of mtDNA and nuclear DNA. DNA methylation-based aging clocks showed a significant relationship with the levels of Klotho, irisin, and its receptor (irisin receptor integrin alpha-V), as well as with epigenetic regulators such as ten-eleven translocation enzyme 2, which were measured using enzyme-linked immunosorbent assay. Therefore, the data suggest a complex regulatory process of epigenetic aging and raise the possibility that D-loop methylation may have functional relevance in health, which remains to be explored. Graphical abstract

  10. Lactate as a Metabolic–Epigenetic Signal Linking High-Intensity Interval Training (HIIT) to miRNA-Centered Remodeling of the Skeletal-Muscle Methylome and Transcriptome. Peer-reviewed

    Zhou L., Mozaffaritabar S., Tanisawa K., Kawamura T., Higuchi M., Boldogh I., Ba X., Goto S., Brooks GA, Gu Y., Radak Z.

    Redox Biology　88　103943　2025/11

    DOI： 10.1016/j.redox.2025.103943 　

  11. Revisiting the relationship between cardiorespiratory fitness and biological aging: Insights from DunedinPACE analysis. Peer-reviewed

    Kawamura T., Nakamura N.:Tabata H., Kawakami R., Usui C., Ito T., Soga K., Taki Y., Radak Z., Torii S., Suzuki K., Ishii K., Sakamoto S., Miyachi M., Oka K., Higuchi M., Muraoka I., Tanisawa K.

    Geroscience　2025/10

    DOI： 10.1007/s11357-025-01930-0 　

  12. Exercise as a geroprotector: focusing on epigenetic aging. Invited Peer-reviewed

    Kawamura T., Higuchi M., Radak Z., Taki Y.

    Aging (Albany NY).　17　(7)　1583-1589　2025/07

    DOI： 10.18632/aging.206278. 　

  13. Organ specificity and commonality of epigenetic aging in low- and high-running capacity rats Peer-reviewed

    Kawamura T., Kerepesi C., Sarkar JP, Torma F., Bori Z., Lei Z., Bakonyi P., Kolonics A., Laszlo B., Higuchi M., Pillár V., Pircs K., Koch LG, Britton SL, Koltai E., Radak Z.

    Aging Cell　24　(8)　e70110　2025/06

    DOI： 10.1111/acel.70110 　

  14. Physical fitness and lifestyles associated with biological aging Invited Peer-reviewed

    Kawamura T, Radak Z, Higuchi M, Tanisawa K

    Aging (Albany NY)　16　(15)　11479-11481　2024/07

    DOI： 10.18632/aging.206031 　

  15. Healthy Japanese Dietary Pattern Is Associated with Slower Biological Aging in Older Men: WASEDA'S Health Study Peer-reviewed

    Kawamura T, Higuchi M, Ito T, Kawakami R, Usui C, McGreevy K.M, Horvath S, Radak Z, Torii S, Suzuki K, Ishii K, Sakamoto S, Oka K, Muraoka I, Tanisawa K

    Frontiers in Nutrition　11　1373806　2024/05

    DOI： 10.3389/fnut.2024.1373806 　

  16. Polygenic risk score, cardiorespiratory fitness, and cardiometabolic risk factors: WASEDA’S Health Study

    Tanisawa K, Tabata H, Nakamura N, Kawakami R, Usui T. Ito T, Kawamura T, Torii S, Suzuki K, Muraoka I, Sakamoto S, Higuchi H, Oka K

    Medicine & Science in Sports & Exercise　56　(10)　2026-2038　2024/04

  17. Effects of palm cooling on thermoregulatory-related and subjective indicators during exercise in a hot environment Peer-reviewed

    Iwata R, Kawamura T, Okabe F, Fujita Z

    Journal of Thermal Biology　120　2024/01

  18. Long-term iron supplementation combined with vitamin B6 enhances maximal oxygen uptake and promotes skeletal muscle-specific mitochondrial biogenesis in rats Peer-reviewed

    Zhou L, Mozaffaritabar S, Kolonics A, Kawamura T, Koike A, Kéringer J, Gu Y, Radak Z, Karabanov R

    Frontiers in Nutrition　10　1335187-1335187　2024/01

  19. The effect of long-term lactate and high-intensity interval training (HIIT) on brain neuroplasticity of aged mice Peer-reviewed

    Zhou L, Mozaffaritabar S, Kawamura T, Koike A, Kolonics A, Kéringer J, Pinho R, Sun J, Shangguan R, Radak Z

    Heliyon　10　(2)　e24421　2024/01

    Publisher: Elsevier BV

    DOI： 10.1016/j.heliyon.2024.e24421 　

    ISSN： 2405-8440

  20. Associations between cardiorespiratory fitness and lifestyle-related factors with DNA methylation-based ageing clocks in older men: WASEDA'S Health Study Peer-reviewed

    Kawamura T*, Radak Z, Tabata H, Akiyama H, Nakamura N, Kawakami R, Ito T, Usui C, Jokai M, Torma F, Kim HK, Miyachi M, Suzuki K, Torii S, Ishii K, Sakamoto S, Oka K, Higuchi M, Muraoka I, McGreevy K.M., Horvath S, Tanisawa K.

    Aging Cell　23　(1)　e13960　2024/01

  21. DNA methylation clock DNAmFitAge shows regular exercise is associated with slower aging and systemic adaptation Peer-reviewed

    Jokai M, Torma F, McGreevy KM, Koltai E, Bori Z, Babszki G, Bakonyi P, Gombos Z, Gyorgy B, Aczel D, Toth L, Osvath P, Fridvalszky M, Teglas T, Ligeti B, Kalcsevszki R, Posa A, Kujach S, Olek R, Kawamura T, Seki Y, Suzuki K, Tanisawa K, Goto S, Boldogh I, Ba X, Szabo D, Davies KJA, Horvath S, Radak Z.

    GeroScience　45　(5)　2805-2817　2023/10

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s11357-023-00826-1 　

    eISSN： 2509-2723

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    Abstract DNAmPhenoAge, DNAmGrimAge, and the newly developed DNAmFitAge are DNA methylation (DNAm)-based biomarkers that reflect the individual aging process. Here, we examine the relationship between physical fitness and DNAm-based biomarkers in adults aged 33–88 with a wide range of physical fitness (including athletes with long-term training history). Higher levels of VO2max (ρ = 0.2, p = 6.4E − 4, r = 0.19, p = 1.2E − 3), Jumpmax (p = 0.11, p = 5.5E − 2, r = 0.13, p = 2.8E − 2), Gripmax (ρ = 0.17, p = 3.5E − 3, r = 0.16, p = 5.6E − 3), and HDL levels (ρ = 0.18, p = 1.95E − 3, r = 0.19, p = 1.1E − 3) are associated with better verbal short-term memory. In addition, verbal short-term memory is associated with decelerated aging assessed with the new DNAm biomarker FitAgeAcceleration (ρ: − 0.18, p = 0.0017). DNAmFitAge can distinguish high-fitness individuals from low/medium-fitness individuals better than existing DNAm biomarkers and estimates a younger biological age in the high-fit males and females (1.5 and 2.0 years younger, respectively). Our research shows that regular physical exercise contributes to observable physiological and methylation differences which are beneficial to the aging process. DNAmFitAge has now emerged as a new biological marker of quality of life.

  22. 自転車運動時における健常若年男性の部位別の皮膚血流量に及ぼす運動強度の影響 Peer-reviewed

    秋山大志, 河村拓史, 村岡功

    運動生理学雑誌　29　(2)　45-52　2022/11

    Publisher: 日本運動生理学会

    ISSN： 1340-3036

  23. Investigation of factors affecting the rate of changes in endurance exercise performance by pre-cooling with ice slurry Peer-reviewed

    Iwata R, Kawamura T, Hosokawa Y, Chang L, Suzuki K, Muraoka I

    Japanese Journal of Physical Fitness and Sports Medicine　71　(4)　345-353　2022/08

    Publisher: The Japanese Society of Physical Fitness and Sports Medicine

    DOI： 10.7600/jspfsm.71.345 　

    ISSN： 0039-906X

    eISSN： 1881-4751

  24. Association between alcohol dietary pattern and prevalence of dyslipidaemia: WASEDA'S Health Study Peer-reviewed

    Tanisawa K, Ito T, Kawakami R, Usui C, Sawada S.S, Kawamura T, Suzuki K, Ishii K, Muraoka I, Sakamoto S, Oka K, Higuchi M

    British Journal of Nutrition　127　(11)　1712-1722　2022/06

  25. Association between dietary patterns and different metabolic phenotypes in Japanese adults: WASEDA’S Health Study. International-journal Peer-reviewed

    Tanisawa K, Ito T, Kawakami R, Usui C, Kawamura T, Suzuki S, Sakamoto S, Ishii K, Muraoka I, Oka K, Higuchi M

    Frontiers in Nutrition　9　779967　2022/01

    DOI： 10.3389/fnut.2022.779967 　

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    Although many studies have reported that a posteriori dietary pattern is associated with metabolic health, there is little evidence of an association between dietary patterns and different metabolic phenotypes. The present study aimed to examine the association between major dietary patterns and different metabolic phenotypes (metabolically healthy non-obese [MHNO], metabolically unhealthy non-obese [MUNO], metabolically healthy obese [MHO], and metabolically unhealthy obese [MUO]) in middle-aged and elderly Japanese adults. This cross-sectional study enrolled 2,170 Japanese adults aged ≥40 years. The four different metabolic phenotypes were determined based on the presence of obesity, abdominal obesity, hypertension, hyperglycemia, and dyslipidemia. The major dietary patterns were determined using principal component analysis based on energy-adjusted food intake. Two dietary patterns were identified: the healthy dietary pattern, which was characterized by a high intake of vegetables, fruits, potatoes, soy products, mushrooms, seaweeds, and fish; and the alcohol dietary pattern, which was characterized by a high intake of alcoholic beverages, liver, chicken, and fish. The healthy dietary pattern was associated with the MHNO and MHO phenotypes (MUNO and MUO as reference groups, respectively), and the multivariate-adjusted odds ratios (ORs) (95% confidence intervals [CIs]) in the highest quartile of healthy dietary pattern score with the lowest quartile as the reference category were 2.10 (1.40-3.15) and 1.86 (1.06-3.25), respectively. Conversely, the alcohol dietary pattern was inversely associated with the MHNO and MHO phenotypes, while the multivariate-adjusted ORs (95% CIs) in the highest quartile of the alcohol dietary pattern score with the lowest quartile as the reference category were 0.63 (0.42-0.94) and 0.45 (0.26-0.76), respectively. There were no significant interactions between sex and healthy/alcohol dietary patterns in the prevalence of the MHNO and MHO phenotypes. In conclusion, the present study's findings suggest that major dietary patterns are associated with different metabolic phenotypes in middle-aged and elderly Japanese adults. These findings provide useful evidence for maintaining metabolic health through diet regardless of obesity status.

  26. Hormone and oxidative stress response during recovery on normoxia after hypoxic resistance exercise Peer-reviewed

    Fujii R, Nakamura N, Kawamura T, Muraoka I

    Gazzetta Medica Italiana - Archivo per le Scienze Medica (In press)　181　(6)　432-438　2022

    DOI： 10.23736/S0393-3660.21.04591-5 　

  27. Hypertrophy of rat skeletal muscle is associated with increased SIRT1/Akt/mTOR/S6 and suppressed Sestrin2/SIRT3/FOXO1 levels Peer-reviewed

    Gombos Z, Koltai E, Torma F, Bakonyi P, Kolonics A, Aczel D, Ditroi T, Nagy P, Kawamura T, Radak Z

    International Journal of Molecular Science　22　(14)　7588　2021/07

  28. Effects of sex differences on breath-hold diving performance Peer-reviewed

    Peng H, Kawamura T, Akiyama H, Chang L, Iwata R, Muraoka I

    Respiratory Physiology and Neurobiology　293　103721　2021/06

  29. Determinants of resting oxidative stress in middle-aged and elderly men and women: WASEDA’S Health Study Peer-reviewed

    Kawamura T, Tanisawa K, Kawakami R, Usui C, Ito T, Tabata H, Nakamura N, Kurosawa S, Choi W, Ma S, Radak Z, Sawada S.S, Suzuki K, Ishii K, Sakamoto S, Oka K, Higuchi M, Muraoka I

    Oxidative Medicine and Cellular Longevity　5566880　2021/05

    DOI： 10.1155/2021/5566880 　

    ISSN： 1942-0900

    eISSN： 1942-0994

  30. Comparison of the effect of post-exercise cooling with ice slurry ingestion between males and females Peer-reviewed

    Iwata R, Kawamura T, Hosokawa Y, Chang L, Suzuki K, Muraoka I

    Journal of Thermal Biology　99　102979　2021/05

  31. Female susceptible to fatigue fracture are resistant to muscle injury: Potential role of COL1A1 variant. Peer-reviewed

    Miyamoto-Mikami E, Kumagai H, Tanisawa K, Taga Y, Hirata K, Kikuchi N, Kamiya N, Kawakami R, Midorikawa T, Kawamura T, Kakigi R, Natsume T, Zempo H, Suzuki K, Kohmura Y, Mizuno K, Torii S, Sakamoto S, Oka K, Higuchi M, Naito H, Miyamoto N, Fuku N

    Medicine & Science in Sports & Exercise　53　(9)　1855-1864　2021/03

    Publisher: Ovid Technologies (Wolters Kluwer Health)

    DOI： 10.1249/mss.0000000000002658 　

    ISSN： 0195-9131

    eISSN： 1530-0315

  32. Visceral fat and cardiorespiratory fitness with prevalence of pre-diabetes/diabetes mellitus among middle-aged and elderly Japanese people: the WASEDA’S Health Study International-journal Peer-reviewed

    Usui C, Kawakami R, Tanisawa K, Ito T, Tabata H, Iizuka S, Kawamura T, Midorikawa T, Sawada S.S, Torii S, Sakamoto S, Suzuki K, Ishii K, Oka K, Muraoka I, Higuchi M

    PLOS ONE　15　(10)　e0241018　2020/10

    DOI： 10.1371/journal.pone.0241018 　

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    The relationships between cardiorespiratory fitness (CRF) measurements not confounded by adiposity and the prevalence of pre-diabetes mellitus (pre-DM) and diabetes mellitus (DM) are not well known. Thus, we aimed to investigate the associations of visceral fat (VF) and CRF with the prevalence of pre-DM/DM among Japanese adults. The study included 970 individuals (327 women and 643 men) who were 40-87 years old and had complete health examinations, abdominal fat area, and fitness data from WASEDA'S Health Study during 2015-2018. The VF area was measured using magnetic resonance imaging. CRF was measured using a cycle ergometer and was defined as VO2peak divided by fat free mass. The pre-DM/DM was identified based on the questionnaire and fasting blood tests. The odds ratios (ORs) and 95% confidence intervals (CIs) for prevalence of pre-DM/DM were calculated. Seventy-three participants had pre-DM and 48 participants had DM. Compared to the low VF group, the high VF group had a higher prevalence of pre-DM/DM (OR: 1.87, 95% CI: 1.18-2.96), although no significant relationship was observed between CRF and pre-DM/DM prevalence (P for trend = 0.239). The sub-group analyses also revealed no significant relationship between CRF and pre-DM/DM prevalence in the low VF group (P for trend = 0.979), although CRF values were inversely related to the prevalence of pre-DM/DM in the high VF group (P for trend = 0.024). Although CRF was not independently related to the prevalence of pre-DM/DM after adjusting for adiposity, higher VF values were related to a higher prevalence of pre-DM/DM. In addition, CRF levels were inversely associated with the prevalence of pre-DM/DM only among high VF individuals.

  33. Differences between sexes in thermoregulatory responses and exercise time during endurance exercise in a hot environment following pre-cooling with ice slurry ingestion Peer-reviewed

    Iwata R, Kawamura T, Hosokawa Y, Chang L, Suzuki K, Muraoka I

    Journal of Thermal Biology　94　102746　2020/10

  34. Thermoregulatory effects of exercising in room temperature combined with a hot water bath for different time durations in a 38°C environment Peer-reviewed

    Chang L, Kawamura T, Fujii R, Muraoka I

    Advances in Exercise and Sports Physiology　26　(2)　15-22　2020/10

  35. Influence of different preceding exercises on fat metabolism during subsequent endurance exercise Peer-reviewed

    Fujii R, Nakamura N, Kawamura T, Ikemura T, Konishi M, Suzuki K, Sakamoto S, Muraoka I

    Gazzetta Medica Italiana - Archivio per le Scienze Mediche　179　(6)　393-403　2020/06

    DOI： 10.23736/S0393-3660.19.04057-9 　

    ISSN： 0393-3660

    eISSN： 1827-1812

  36. The effects of sex differences on delayed-onset muscle soreness by cold-water immersion following resistance exercise Peer-reviewed

    Iwata R, Chang L, Akiyama H, Kawamura T, Suzuki K, Sakamoto S, Muraoka I

    Gazzetta Medica Italiana - Archivio per le Scienze Mediche　181　(1-2)　80-89　2020/02

    DOI： 10.23736/S0393-3660.20.04328-4 　

  37. Application of molecular hydrogen as a novel antioxidant in sports science International-journal Peer-reviewed

    Kawamura T, Higashida K, Muraoka I

    Oxidative Medicine and Cellular Longevity　2020　2328768　2020/01

    DOI： 10.1155/2020/2328768 　

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    Molecular hydrogen (H2) is a colorless, tasteless, odorless, and minimal molecule with high flammability. Although H2 has been thought to be an inert gas in living bodies for many years, an animal study reported that inhalation of H2 gas decreased oxidative stress and suppressed brain injury caused by ischemia and reperfusion injury due to its antioxidant action. Since then, the antioxidant action of H2 has attracted considerable attention and many studies have reported on its benefits. Most studies have reported the effects of H2 on diseases such as cancer, diabetes, cerebral infarction, and Alzheimer's disease. However, little is known regarding its effects on healthy subjects and exercise. Thus far, including our study, only 6 studies have explored the effect of H2 on exercise. H2 is the smallest molecule and therefore can easily penetrate the cellular membrane and rapidly diffuse into organelles. H2 is thought to be able to selectively reduce hydroxyl radicals and peroxynitrite and does not affect physiologically reactive species. H2 can be supplied to the body through multiple routes of administration, such as oral intake of H2 water and H2 bathing. Therefore, H2 may be a potential alternative strategy for conventional exogenous antioxidant interventions in sports science. The purpose of this review is to provide evidence regarding the effects of H2 intake on changes in physiological and biochemical parameters, centering on exercise-induced oxidative stress, for each intake method. Furthermore, this review highlights possible future directions in this area of research.

  38. Hydrogen water intake may suppress liver glycogen utilization without affecting redox biomarkers during exercise in rats Peer-reviewed

    Kawamura T, Fujii R, Higashida K, Muraoka I

    Gazzetta Medica Italiana - Archivio per le Scienze Mediche　178　(9)　611-617　2019/09

  39. Involvement of Neutrophil Dynamics and Function in Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness: Effect of Hydrogen Bath International-journal Peer-reviewed

    Kawamura T, Suzuki K, Takahashi M, Tomari M, Hara R, Gando Y, Muraoka I

    Antioxidants　7　(10)　127　2018/09

    DOI： 10.3390/antiox7100127 　

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    The purpose of this study was to investigate the involvement of neutrophil dynamics and function in exercise-induced muscle damage (EIMD) and delayed-onset muscle soreness (DOMS), and the effect of molecular hydrogen (H₂) intake on these parameters. Nine healthy and active young men performed H₂ and placebo bath trial in a crossover design. They carried out downhill running (-8% slope) for 30 min at a speed corresponding to 75~85% of peak oxygen uptake (VO₂peak). Subsequently, they repeated bathing for 20 min per day for one week. Degree of muscle soreness (visual analogue scale: VAS), peripheral leukocyte counts, neutrophil dynamics and function, muscle damage, and inflammation markers were measured. Plasma interleukin (IL)-6 concentration was significantly correlated with peripheral neutrophil count, VAS, and serum creatine kinase activity, respectively, after downhill running. Peripheral neutrophil count and serum myoglobin concentration were also significantly correlated. Conversely, there were no effects of H₂ bath. These results suggest that IL-6 may be involved in the mobilization of neutrophils into the peripheral blood and subsequent EIMD and DOMS after downhill running; however, it is not likely that H₂ bath is effective for the inflammatory process that was centered on neutrophils after downhill running.

  40. Exercise-Induced Oxidative Stress and the Effects of Antioxidant Intake from a Physiological Viewpoint. Peer-reviewed

    Kawamura T, Muraoka I

    Antioxidants　7　(9)　119　2018/09

    DOI： 10.3390/antiox7090119 　

  41. Effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle Peer-reviewed

    Kawamura T, Fujii R, Li X, Higashida K, Muraoka I

    Science and Sports　33　(3)　169-175　2018/06

    Publisher: Elsevier Masson SAS

    DOI： 10.1016/j.scispo.2017.08.008 　

    ISSN： 1778-4131 0765-1597

  42. Time course of decrease in skeletal muscle mitochondrial biogenesis after discontinuation of high-fat diet Peer-reviewed

    Li X, Higashida K, Kawamura T, Higuchi M

    Journal of Nutritional Science and Vitaminology　64　(3)　233-238　2018/01

    DOI： 10.3177/jnsv.64.233 　

    ISSN： 0301-4800

  43. Alternate-Day High-Fat Diet Induces an Increase in Mitochondrial Enzyme Activities and Protein Content in Rat Skeletal Muscle Peer-reviewed

    Li X, Higashida K, Kawamura T, Higuchi M

    Nutrients　8　(4)　203　2016/04

    DOI： 10.3390/nu8040203 　

    ISSN： 2072-6643

  44. Effects of hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness Peer-reviewed

    Takuji Kawamura, Yuko Gando, Masaki Takahashi, Reira Hara, Katsuhiko Suzuki, Isao Muraoka

    Japanese Journal of Physical Fitness and Sports Medicine　65　(3)　297-305　2016

    Publisher: Japanese Society of Physical Fitness and Sports Medicine

    DOI： 10.7600/jspfsm.65.297 　

    ISSN： 0039-906X

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Misc. 3

  1. Combined NAD(P)H and lipofuscin FLIM revealed the development of metabolic syndrome in the liver of epigenetically altered rats Peer-reviewed

    Kolonics A, Kawamura T, K, Szipocs R, Radak Z

    Single Molecule Spectroscopy and Superresolution Imaging XVII　1284909　2024/03

  2. Cardiorespiratory fitness and visceral fat on prevalent diabetes in Japanese adults: WASEDA'S Health Study

    Usui Chiyoko, Kawakami Ryoko, Tanisawa Kumpei, Tabata Hiroki, Ito Tomoko, Iizuka Satoshi, Kawamura Takuji, Midorikawa Taishi, Sawada Susumu, Torii Suguru, Sakamoto Shizuo, Suzuki Katsuhiko, Ishii Kaori, Oka Koichiro, Higuchi Mitsuru

    The Journal of Physical Fitness and Sports Medicine　8　(6)　364-364　2019/11

    Publisher: (一社)日本体力医学会

    ISSN： 2186-8131

    eISSN： 2186-8123

  3. Alternate-day High-fat Diet Feeding Induces Insulin resistance Despite No Abdominal Fat Accumulation And Mitochondrial Dysfunction

    Kazuhiko Higashida, Takuji Kawamura, Isao Muraoka, Mitsuru Higuchi

    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE　47　(5)　411-412　2015/05

    DOI： 10.1249/01.mss.0000477554.44047.b8 　

    ISSN： 0195-9131

    eISSN： 1530-0315

Presentations 34

  1. Circulating BDNF is associated with attenuated inflammation-related neuroaxonal injury while tau-related biomarkers remain additive.

    Shi Y., Kawamura T., Oba K., Taki Y.

    Federation of European Neuroscience Societies Forum 2026　2026/07/06

  2. Regulation of epigenetic aging through exercise and nutrition: Multifaceted insights from human and animal models Invited

    Kawamura T.

    The 49th Annual Meeting of the Japan Society for Biomedical Gerontology. Congress Organizing Symposium: "Biological Aging Control Reorganized by Exercise and Nutrition: From Basic Mechanisms to Applied Developments"　2026/06/28

  3. The Association Between Physical Activity and Humor Orientation in Young Adults

    Soga K., Oba K., Kawamura T., Ebina K., Yamaya N., Takeuchi H., Taki Y.

    The 28th Annual Meeting of the Japan Society of Exercise Epidemiology (Accepted)　2026/05/30

  4. Regulation of the Aging Clock by Exercise and Its Social Implementation

    Kawamura T.

    Study Group, Healthcare Industries Division, Ministry of Economy, Trade and Industry (METI)　2026/05/19

  5. A Preliminary Study on the Effects of Using Voice-Based Conversational AI Capable of Sharing Memories on Cognitive and Psychological Functions

    Oba K., Uno A., Takahashi M., Soga K., Kawamura T., Shinada T., Taki Y.

    Japanese Society of Health Psychology　2025/09/20

  6. Effects of Exercise Training on Biological Aging and Its Interindividual Variability

    Kawamura T., da Silva Rodrigues G., Soga K., Higuchi M., Taki Y., da Silva Sobrinho AS, Noronha NY, de Lima JGR, Roberto Bueno, únior

    The 79th Annual meeting of Japanese Society of Physical Fitness and Sports Medicine　2025/09/17

  7. The effects of regular circuit training on memory function in middle-aged and older women and its relationship with the Gut Microbiome

    Soga K., Takahashi Y., Kawashima K., Kawamura T., Uno A., Oba K., Taki Y.

    The 79th Annual Meeting of Japanese Society of Physical Fitness and Sports Medicine　2025/09/18

  8. Lifestyle determines biological age

    Kawamura T.

    The 79th Annual Meeting of Japanese Society of Physical Fitness and Sports Medicine: Luncheon Seminar: “Healthy Longevity Through Joy of Movement and Joy of Eating ―WASEDA’S Health Study―”　2025/09/18

  9. Physical activity with Friends, Humor, and Loneliness in Older Adults: Examining Direct and Indirect Pathways to Psychological Distress

    Soga K., Uno A., Kawamura T., Kamijo K., Taki Y.

    The 79th Annual Meeting of Japanese Society of Physical Fitness and Sports Medicine　2025/09/18

  10. Understanding and Predicting Mental Health in Older Adults: A Large-Scale Bayesian Neural Network Analysis of Physical Activity and Social Skills

    Soga K., Uno A., Kawamura T., Kamijo K., Taki Y.

    The 79th Annual meeting of Japanese Society of Physical Fitness and Sports Medicine　2025/09/17

  11. Can exercise reverse aging? Regulating biological aging from the perspective of DNA methylation aging clocks Invited

    Kawamura T.

    The 40th SAM Conference, Symposium “Understanding and Regulating Aging I”　2025/08/29

  12. Lifestyle interventions mitigate genetic susceptibility to late-onset Alzheimer’s disease.

    Shi Y., Kawamura T., Liu Y., Oba K., Taki Y.

    Alzheimer’s Association International Conference.　2025/07

  13. Social physical activity, humor expression, and loneliness: a cross-sectional study of their relationships.

    Soga K., Uno A., Kawamura T., Kamijo K.

    European college of sports science 30th annual congress　2025/07

  14. Healthy lifestyle mitigates genetic risk of Alzheimer's disease in older adults.

    Shi Y., Kawamura T., Liu Y., Oba K., Du Y., Taki Y.

    2025/01/14

  15. The effect of long-term lactate and high-intensity interval training (HIIT) on brain neuroplasticity of aged mice

    Zhou L, Mozaffaritabar S, Kawamura T, Koike A, Kolonics A, Kéringer J, Pinho R, Sun J, Shangguan R, Radak Z

    International Biochemistry and Exercise Conference　2024/07/10

  16. Polygenic risk score, cardiorespiratory fitness, and cardiometabolic risk factors: WASEDA’S Health Study

    Tanisawa K, Tabata H, Nakamura N, Kawakami R, Usui T. Ito T, Kawamura T, Torii S, Suzuki K, Muraoka I, Sakamoto S, Higuchi H, Oka K

    International Biochemistry and Exercise Conference　2024/07/10

  17. Organ specificity and consistency of epigenetic aging in low- and high-running capacity rats

    Kawamura T, Kerepesi C, Torma F, Boli Z, Lei Z, Bakonyi P, Kolonics A, Laszlo B, Koch LG, Britton SL, Koltai E, Radak Z

    International Biochemistry and Exercise Conference　2024/07/09

  18. Combined NAD(P)H and Lipofuscin FLIM revealed the development of metabolic syndrome in the liver of epigenetically altered rats.

    Koloncs A, Kawamura T, Szipőcs R, Radak Z

    SPIE Photonics West technical conferences　2024/01

  19. ポリジェニックリスクスコアと心血管代謝リスクの関連は全身持久力により 修飾されるか？

    谷澤薫平, 田端宏樹, 中村宣博, 川上諒子, 薄井澄誉子, 伊藤智子, 河村拓史, 鳥居俊, 鈴木克彦, 村岡功, 坂本静男, 樋口満, 岡浩一朗

    第2回スポーツ遺伝学研究会　2023/08

  20. Accelerated epigenetic changes may contribute to the development of hepatic insulin resistance revealed by NADH FLIM

    Koloncs A, Kawamura T, Szipőcs R, Radak Z

    15th MiPconference: Bioenergetics　2023/07

  21. 暑熱環境下での運動後におけるアイススラリー 摂取が身体冷却に及ぼす影響の性差

    岩田理沙, 河村拓史, 細川由梨, 張黎栗, 鈴木克彦, 村岡功

    第76回日本体力医学会大会　2021/09

  22. Effects of sex differences on exercise capacity and thermoregulation to pre-cooling with ice slurry ingestion in a hot environment.

    Iwata R, Chang L, Kawamura T, Suzuki K, Muraoka I

    European college of sports science 25th annual congress　2020/09

  23. 日本人中高齢者における全身持久力および内臓脂肪と糖尿病有病率の関係：WASEDA’S Health Study

    薄井 澄誉子, 川上 諒子, 谷澤 薫平, 田端 宏樹, 伊藤 智子, 飯塚 哲司, 河村 拓史, 緑川 泰史, 澤田 亨, 鳥居 俊, 坂本 静男, 鈴木 克彦, 岡 浩一朗, 樋口 満

    第74回日本体力医学会　2019/09

  24. 常温環境下での運動トレーニングと温水浴の組み合わせが体温調節機能と運動パフォーマンスに及ぼす影響

    張 黎栗, 河村 拓史, 藤井 亮輔, 村岡 功

    第74回日本体力医学会　2019/09

  25. 水素水摂取はラットの運動時におけるレドックスバイオマーカーに影響を与えずに肝グリコーゲン利用を抑制する

    河村 拓史, 藤井 亮輔, 東田 一彦, 村岡 功

    第73回日本体力医学会　2018/09

  26. The WASEDA'S Health Study focusing on redox inter-individual variability during exercise in elderly

    Takuji Kawamura

    MIRAI Ageing Workshop　2018/06

  27. Hydrogen water suppresses skeletal muscle and liver glycogenolysis during exercise while does not affect oxidative stress, blood energy substrate and endurance performance in rats International-presentation

    Takuji Kawamura, Ryosuke Fujii, Kazuhiko Higashida, Isao Muraoka

    European College of Sports Science 22nd annual congress　2017/07

  28. Effects of hydrogen water on exercise-induced oxidative stress, energy metabolism and endurance performance in rats

    Takuji Kawamura, Ryosuke Fujii, Kazuhiko Higashida, Isao Muraoka

    international symposium of SGU health and sports science model　2017/03

  29. Effects of different exhaustive exercise on oxidative stress markers in rat plasma ans skeletal muscle

    Takuji Kawamura, Ryosuke Fujii, Xi li, Kazuhiko Higashida, Isao Muraoka

    The Journal of Physical Fitness and Sports Medicine　2016/09

  30. Effects of different pre-exercises on lipolysis

    Ryosuke Fujii, Nobuhiro Nakamura, Takuji Kawamura, Tsukasa Ikemura, Masayuki Konishi, Katsuhiko Suzuki, Shizuo Sakamoto, Isao Muraoka

    2016/09

  31. 運動時の酸化ストレスおよび遅発性筋痛に及ぼす水素の影響

    河村 拓史, 丸藤 祐子, 高橋 将記, 原 怜来, 鈴木 克彦, 村岡 功

    第70回日本体力医学会　2015/09

  32. 水素摂取が一過性持久運動時の酸化ストレスに及ぼす影響

    河村 拓史, 藤井 亮輔, 東田 一彦, 村岡 功

    第2回アクティブエイジング研究所 研究報告会　2015/03

  33. Alternate-day high-fat diet feeding induces insulin resistance despite no abdominal fat accumulation and mitochondrial dysfunction International-presentation Invited

    Kazuhiko Higashida, Takuji Kawamura, Mitsuru Higuchi

    Medicine and Science in Sports and Exercise　2014/05

  34. The Convergence Point of Aging Indicators: From Diverse Metrics to “ Usable Criteria”. Invited

    Takuji Kawamura

    The 4th Aging Clocks Workshop, Panel Discussion　2025/11/29

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Research Projects 6

  1. Lifestyle Habits and Social Environments Supporting Social Development and Their Long-Term Neurobiological and Molecular Mechanisms

    Ishihara Toru, Kawamura Takuji, Takagishi Haruto

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Smart-Aging Research Center, Institute of Development, Aging and Cancer, Tohoku University

    2026/04 - 2030/03

  2. Verification of Rejuvenation Effects of Exercise on Epigenetic Drift

    Higuchi Mitsuru, Tanisawa Kumpei, Suzuki Katsuhiko, Kawamura Takuji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research B

    Institution: Smart-Aging Research Center, Institute of Development, Aging and Cancer, Tohoku University

    2024/04 - 2028/03

  3. 老化血液メチロームに対する心肺体力の影響に性差はあるのか？

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 若手研究

    Institution: 東北大学スマート・エイジング学際重点研究センター

    2025/04 - 2027/03

  4. 運動により分泌されるエクソソームの機能解析と生理的役割の解明

    鈴木 克彦, 川西 範明, 河村 拓史

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B))

    Category: 国際共同研究加速基金(国際共同研究強化(B))

    Institution: 早稲田大学

    2020/10/27 - 2024/03/31

    More details Close

    2年目には、マウスから採取した骨格筋組織を用いたex vivo実験を行い、培養骨格筋から分泌される2年目には、マウスから採取した骨格筋組織を用いたex vivo実験を行い、培養骨格筋から分泌されるエクソソームを対象としたRNAの網羅的な発現解析法を確立した。加齢などにより異常を引き起こした骨格筋ではエクソソーム分泌動態も異常をきたしていることが想定され、分泌異常が様々な病態を引き起こしている可能性があることから、加齢モデルマウスの骨格筋から分泌されるエクソソームを対象として質的な変動解析を行った。4カ月齢の若齢マウスおよび27カ月齢の高齢マウスから採取した培養骨格筋由来のエクソソームのmicroRNAおよびmRNAの差次的発現解析を実施した結果、加齢により発現が増減する特異的なmicroRNA25種類およびmRNA370種類を見出した（第77回日本体力医学会発表発表予定、論文準備中）。KEGG パスウェイ解析により、 加齢により増加するmicroRNAはFatty acid metabolismなどの代謝経路に影響を及ぼすことが示された。 また、これまでに我々は電気刺激による筋収縮負荷がマウス血清エクソソームのmicroRNAの発現プロファイルを変化させることを明らかにしてきた（第76回日本体力医学会発表、論文投稿中）。2年目には、筋収縮刺激により分泌される血清エクソソームが脳神経細胞の機能に影響を及ぼす影響について検証した。マウス胎児海馬由来の初代神経細胞に血清エクソソームを添加することによる mRNA の差次的発現解析を実施した結果、筋収縮負荷のマウス血清エクソソームは 132 種類の mRNA の発現を変化させることを見出した（論文準備中）。また、KEGG パスウェイ解析により、神経細胞の増殖・分化に関連する Hippo シグナル伝達経路に影響を及ぼすことが示された。

  5. Effect of long-term exercise training on epigenetic clock

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Category: Grant-in-Aid for Early-Career Scientists

    Institution: Waseda University

    2020/04/01 - 2023/03/31

  6. Whether the molecular hydrogen improve endurance performance by decreasing oxidative stress?

    Muraoka Isao, Higashida Kazuhiko, Ikemura Tsukasa, Kawamura Takuji, Fujii Ryosuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Waseda University

    2015/04/01 - 2018/03/31

    More details Close

    The purpose of this research was to obtain basic knowledge for application of molecular hydrogen (H2), which is attracting attention as a new antioxidant agent in recent years, in the health and sports science field. As a result of this study, there was no effect of H2 intake on exercise-induced oxidative stress, and endurance performance. However, it was shown that H2 significantly suppresses the decrease in liver glycogen content during endurance exercise. These evidences suggest that intake of H2 suppresses the utilization of liver glycogen during exercise via physiological action other than antioxidation. Further research is needed on the mechanism that H2 suppressed the utilization of liver glycogen.

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Teaching Experience 7

  1. 加齢・老年病科第3次臨床修練　東北大学加齢老年病科

  2. Topics and Discussion: Exercise, Aging and Epigenetics　Tohoku University, School of Medicine

  3. Biological Aging and How to Control It: The Cutting Edge of Aging Biomarker Research　Graduate School of Medicine, Tohoku University

  4. 運動生理学演習　城西大学薬学部

  5. 運動生理学　城西大学現代政策学部

  6. スポーツ教養演習Ⅱ　早稲田大学スポーツ科学学術院

  7. スポーツ教養演習I　早稲田大学スポーツ科学学術院

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Media Coverage 13

  1. LONGEVITY: The Long Game. 7 recent studies that examine the biological, cognitive and behavioural factors that extend our life and healthspan.

    Zoomer (Canada)　Zoomer Magazine　https://zoomer.com/health/2026/01/28/the-long-game

    2026/01/28

    Type: Newspaper, magazine

  2. Japan Post Co., Ltd. has entered into a joint research agreement with Tohoku University’s Smart-Aging Research Center and Otsuka Pharmaceutical Co., Ltd. on the regulation of aging clocks through exercise.

    The Sankei Shimbun　https://www.sankei.com/pressrelease/prtimes/Q2ZYAC4VGJIOHLKT7YDRZHNYF4/

    2025/12

    Type: Internet

  3. Continuous exercise slows aging at the molecular level: Research findings

    WIRED　https://wired.jp/article/exercise-reverse-epigenetic-aging/

    2025/09/13

    Type: Internet

  4. 健康的な日本の食事習慣は、欧米の食事習慣と比較して老化を遅らせる 高齢男性144名を調査 早稲田大学

    スポーツ栄養Web　https://sndj-web.jp/news/002857.php

    2024/07/14

    Type: Internet

  5. 心肺体力の維持は老化の遅延に関連、早稲田大学が調査

    News picks　https://newspicks.com/news/8873720/

    2023/09/06

    Type: Internet

  6. 心肺体力の維持は老化の遅延に関連、早稲田大学が調査

    大学ジャーナルオンライン　https://univ-journal.jp/234131/

    2023/09/06

    Type: Internet

  7. 心肺能力の向上は老化を遅らせることができるか

    バイオの社　https://pat.intellectual-info.com/bio-mori/column/30685/

    2023/09/03

    Type: Internet

  8. 心肺体力維持と老化の関係を解明―早稲田大学

    アンドラ　https://andla.jp/wp/?p=37773

    2023/09/01

    Type: Internet

  9. Cardiopulmonary fitness maintenance is associated with delayed biological aging (Waseda University)

    https://healthy-life21.com/2023/08/31/20230831/

    2023/08/31

    Type: Internet

  10. 心肺体力維持と老化の関係を解明～心肺体力維持が生物学的老化の「遅延」と「進行」に強く関係～

    Tii生命科学

    2023/08/30

    Type: Internet

  11. 早稲田大、心肺体力維持が生物学的老化の「遅延」と「進行」に強く関係

    日経バイオテク　https://bio.nikkeibp.co.jp/atcl/release/23/08/30/17576/

    2023/08/30

    Type: Internet

  12. 早稲田大、心肺体力維持が生物学的老化の「遅延」と「進行」に強く関係.

    日経バイオテク　https://bio.nikkeibp.co.jp/atcl/release/23/08/30/17576/

    2023/08/30

    Type: Internet

  13. 早大、心肺体力の維持がもたらす生物学的老化の「遅延」と「進行」の関係を解明

    日本経済新聞オンライ ン　https://www.nikkei.com/article/DGXZRSP661361_Q3A830C2000000/?msockid=35ceda1c13076ccf0945ceed125f6dbb

    2023/08/30

    Type: Internet

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Academic Activities 1

  1. Review (（Aging Cell, Environmental Epigenetics, Annals of Medicine, Canadian Journal of Physiology and Pharmacology, Frontiers in Nutrition, PLoS One etc.）

    Activity type: Peer review