Details of the Researcher

PHOTO

Masako Toda
Section
Graduate School of Agricultural Science
Job title
Professor
Degree

  • 博士（農学）（東京大学）

  • 修士（農学）（信州大学）

e-Rad No.
10828429

Research History 9

  • 2022/01 - Present
    福島大学 食農学類 発酵醸造研究所 特任教授（クロスアポイントメント）

  • 2018/04 - Present
    Tohoku University　Graduate School of Agricultural Science

  • 2014/01 - 2018/03
    ドイツ連邦保健省ポール・エーリッヒ研究所 分子アレルギ－学 副ユニット長

  • 2005/12 - 2013/12
    ドイツ連邦保健省ポール・エーリッヒ研究所 若手研究グループ 実験アレルギー学 グループリーダー

  • 2004/03 - 2005/11
    ユニバーシティー・カレッジ・ロンドン、眼科研究所、免疫部 リサーチアソシエイト

  • 2002/03 - 2004/02
    ユニバーシティー・カレッジ・ロンドン、眼科研究所、免疫部 科学技術振興機構海外若手派遣研究員

  • 2001/07 - 2002/02
    ユニバーシティー・カレッジ・ロンドン、眼科研究所、免疫部 博士研究員

  • 1999/04 - 2001/06
    東京大学大学院、農学生命科学研究科、応用生命化学専攻 農学特定研究員

  • 1997/04 - 1999/03
    国立感染症研究所、免疫部、ヒューマンサイエンス財団流動研究員

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Education 3

  • 東京大学大学院農学生命科学研究科博士課程後期

    1994/04 - 1997/03

  • 信州大学大学院、農学系研究科 修士課程

    1992/04 - 1994/03

  • Shinshu University　Faculty of Agriculture

    1988/04 - 1992/03

Committee Memberships 11

  • 消費者庁 食品衛生基準審議会 食品規格・乳肉水産・伝達性海綿状脳症対策部会委員

    2025/01 - Present

  • 仙台市食品安全対策協議会委員

    2023/04 - Present

  • バイオサイエンスとインダストリー（B&I） 編集委員

    2023/04 - Present

  • 日本農芸化学会 理事（出版担当）

    2023/04 - Present

  • 日本食品免疫学会 広報編集委員

    2022/04 - Present

  • 日本栄養・食糧学会 代議員

    2020/04 - Present

  • Journal of Agricultural and Food Chemistry　Editorial Advisory Board Member

    2019/01 - 2025/05

  • 厚生労働省 薬事・食品衛生審議会 食品規格部会委員

    2019/01 - 2024/12

  • 第34回日本メイラード学会年会 世話人

    2023/11 - 2024/10

  • The 3rd international conference of the Food Allergy Forum Advisory Board Member

    2020/04 - 2023/12

  • 日本農芸化学会 和文誌編集委員

    2021/04 - 2023/03

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Professional Memberships 5

  • 日本ビタミン学会

  • 食品免疫学会

  • JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY

  • 日本栄養食糧学会

  • JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

Research Interests 4

  • 食品科学

  • 低アレルゲン化分子の開発

  • アレルギ－

  • 食品免疫学

Research Areas 1

  • Life sciences / Food sciences /

Papers 77

  1. Dietary fiber pectin alters the gut microbiota and diminishes the inflammatory immune responses in an experimental peach allergy mouse model Peer-reviewed

    Hanna Steigerwald, Melanie Albrech, Birgit Blissenbach, Maren Krause, Andrea Wangorsch, Maike Scho, Irene Gonzalez-Menendez, Leticia Quintanilla-Martinez, Masako Toda, Stefan Vieths, Oleg Kru, Stephan Scheurer, Frank Blanco-Pérez

    Scientific Report (国際共著論文)　14　(30503)　2024/12

  2. Characterization of the Immune-Modulating Properties of Different β-Glucans on Myeloid Dendritic Cells. International-journal Peer-reviewed

    Hannah Rainer, Alexandra Goretzki, Yen-Ju Lin, Hannah Ruth Schiller, Maren Krause, Sascha Döring, Daniel Strecker, Ann-Christine Junker, Sonja Wolfheimer, Masako Toda, Stephan Scheurer, Stefan Schülke

    International journal of molecular sciences（国際共著論文）　25　(18)　2024/09

    DOI： 10.3390/ijms25189914 　

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    In allergen-specific immunotherapy, adjuvants are explored for modulating allergen-specific Th2 immune responses to re-establish clinical tolerance. One promising class of adjuvants are β-glucans, which are naturally derived sugar structures and components of dietary fibers that activate C-type lectin (CLR)-, "Toll"-like receptors (TLRs), and complement receptors (CRs). We characterized the immune-modulating properties of six commercially available β-glucans, using immunological (receptor activation, cytokine secretion, and T cell modulating potential) as well as metabolic parameters (metabolic state) in mouse bone marrow-derived myeloid dendritic cells (mDCs). All tested β-glucans activated the CLR Dectin-1a, whereas TLR2 was predominantly activated by Zymosan. Further, the tested β-glucans differentially induced mDC-derived cytokine secretion and activation of mDC metabolism. Subsequent analyses focusing on Zymosan, Zymosan depleted, β-1,3 glucan, and β-1,3 1,6 glucan revealed robust mDC activation with the upregulation of the cluster of differentiation 40 (CD40), CD80, CD86, and MHCII to different extents. β-glucan-induced cytokine secretion was shown to be, in part, dependent on the activation of the intracellular Dectin-1 adapter molecule Syk. In co-cultures of mDCs with Th2-biased CD4+ T cells isolated from birch allergen Bet v 1 plus aluminum hydroxide (Alum)-sensitized mice, these four β-glucans suppressed allergen-induced IL-5 secretion, while only Zymosan and β-1,3 glucan significantly suppressed allergen-induced interferon gamma (IFNγ) secretion, suggesting the tested β-glucans to have distinct effects on mDC T cell priming capacity. Our experiments indicate that β-glucans have distinct immune-modulating properties, making them interesting adjuvants for future allergy treatment.

  3. The impact of high-IgE levels on metabolome and microbiome in experimental allergic enteritis. International-journal Peer-reviewed

    Elisa Zubeldia-Varela, Frank Blanco-Pérez, Tomás Clive Barker-Tejeda, David Rojo, Alma Villaseñor, Jahidul Islam, Irene Gonzalez-Menendez, Jonathan Laiño, Maren Krause, Hanna Steigerwald, Manuela Martella, Leticia Quintanilla-Martinez, Philipp Yu, Coral Barbas, Stefan Vieths, Tomonori Nochi, Domingo Barber, Masako Toda, Marina Pérez-Gordo

    Allergy (国際共著論文)　2024/06

    DOI： 10.1111/all.16202 　

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    BACKGROUND: The pathological mechanism of the gastrointestinal forms of food allergies is less understood in comparison to other clinical phenotypes, such as asthma and anaphylaxis Importantly, high-IgE levels are a poor prognostic factor in gastrointestinal allergies. METHODS: This study investigated how high-IgE levels influence the development of intestinal inflammation and the metabolome in allergic enteritis (AE), using IgE knock-in (IgEki) mice expressing high levels of IgE. In addition, correlation of the altered metabolome with gut microbiome was analysed. RESULTS: Ovalbumin-sensitized and egg-white diet-fed (OVA/EW) BALB/c WT mice developed moderate AE, whereas OVA/EW IgEki mice induced more aggravated intestinal inflammation with enhanced eosinophil accumulation. Untargeted metabolomics detected the increased levels of N-tau-methylhistamine and 2,3-butanediol, and reduced levels of butyric acid in faeces and/or sera of OVA/EW IgEki mice, which was accompanied with reduced Clostridium and increased Lactobacillus at the genus level. Non-sensitized and egg-white diet-fed (NC/EW) WT mice did not exhibit any signs of AE, whereas NC/EW IgEki mice developed marginal degrees of AE. Compared to NC/EW WT mice, enhanced levels of lysophospholipids, sphinganine and sphingosine were detected in serum and faecal samples of NC/EW IgEki mice. In addition, several associations of altered metabolome with gut microbiome-for example Akkermansia with lysophosphatidylserine-were detected. CONCLUSIONS: Our results suggest that high-IgE levels alter intestinal and systemic levels of endogenous and microbiota-associated metabolites in experimental AE. This study contributes to deepening the knowledge of molecular mechanisms for the development of AE and provides clues to advance diagnostic and therapeutic strategies of allergic diseases.

  4. Effects of pectin methyl-esterification on intestinal microbiota and its immunomodulatory properties in naive mice Peer-reviewed

    Hanna Steigerwald, Frank Blanco-Pérez, Andrea Macías-Camero, Melanie Albrech, Melanie Huch, Caroline Bender, Stefan Schülke, Judith Keller, Maren Krause, Carol Barbas, Irene Gonzalez-Menendez, Leticia Quintanilla-Martinez, Masako Toda, Domingo Barber, Sabine Kulling, Mirko Bunzel, Stefan Vieths, Alma Villaseñor, Dominic Stoll, Stephan Scheurer

    Carbohydrate Polymers (国際共著論文)　2024/03

    DOI： 10.1016/j.carbpol.2024.122007 　

  5. β-1,3-glucan, but not β-1,3/1,6-glucan, exacerbates experimental food allergy, while both increase IgA induction. International-journal Peer-reviewed

    Chaoqi He, Yunhui Liu, Stefan Schülke, Shunsuke Nishio, Yingnan Guo, Hannah Rainer, Krause Maren, Ting-Yu Cheng, Tomonori Nochi, Stefan Vieths, Stephan Scheurer, Tsukasa Matsuda, Masako Toda

    Allergy (国際共著論文)　79　(2)　503　2024/02

    DOI： 10.1111/all.15841 　

  6. Functional components of walnuts: a review focusing on native and cultivated species. Peer-reviewed

    Ritsuko Fukasawa, Taiki Miyazawa, Chizumi Abe, Maharshi Bhaswant, Masako Toda

    Food Science and Technology Research　30　1-12　2024/01

    Publisher: Japanese Society for Food Science and Technology

    DOI： 10.3136/fstr.FSTR-D-23-00093 　

    ISSN： 1344-6606

    eISSN： 1881-3984

  7. Analysis of oxidized glucosylceramide and its effects on altering gene expressions of inflammation induced by LPS in intestinal tract cell models. International-journal Peer-reviewed

    Mirinthorn Jutanom, Shunji Kato, Shinji Yamashita, Masako Toda, Mikio Kinoshita, Kiyotaka Nakagawa

    Scientific reports　13　(1)　22537-22537　2023/12/18

    DOI： 10.1038/s41598-023-49521-3 　

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    Glucosylceramide (GlcCer) belongs to sphingolipids and is found naturally in plant foods and other sources that humans consume daily. Our previous studies demonstrated that GlcCer prevents inflammatory bowel disease both in vitro and in vivo, whose patients are increasing alarmingly. Although some lipids are vulnerable to oxidation which changes their structure and activities, it is unknown whether oxidative modification of GlcCer affects its activity. In this research, we oxidized GlcCer in the presence of a photosensitizer, analyzed the oxide by mass spectrometric techniques, and examined its anti-inflammatory activity in lipopolysaccharide (LPS)-treated differentiated Caco-2 cells as in vitro model of intestinal inflammation. The results showed that GlcCer is indeed oxidized, producing GlcCer hydroperoxide (GlcCerOOH) as a primary oxidation product. We also found that oxidized GlcCer preserves beneficial functions of GlcCer, suppressing inflammatory-related gene expressions. These findings suggested that GlcCerOOH may perform as an LPS recognition antagonist to discourage inflammation rather than induce inflammation.

  8. Quantification and Comparison of Nutritional Components in Oni Walnut (Juglans ailanthifolia Carr.), Hime Walnut (Juglans subcordiformis Dode.), and Cultivars Peer-reviewed

    Ritsuko Fukasawa, Taiki Miyazawa, Chizumi Abe, Maharshi Bhaswant, Masako Toda

    Horticulturae　9　(11)　1211-1221　2023/11

    Publisher: MDPI AG

    DOI： 10.3390/horticulturae9111221 　

    eISSN： 2311-7524

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    Walnuts are rich sources of lipids and polyunsaturated fatty acids and are expected to promote health. There are two Japanese native walnut species: Oni walnut (Juglans ailanthifolia Carr.) and Hime walnut (Juglans subcordiformis Dode.). However, despite the fact that these Japanese native walnuts have long been consumed in local cuisine, their nutritional composition is largely unknown. This study aimed to assess the concentrations of total lipids, and fatty acid composition including polyunsaturated fatty acids, in the kernels of Oni walnut and Hime walnut. In addition, we assessed various aspects related to their nutritional and functional values, by measuring the total protein, amino acids, minerals, and total polyphenols. The concentrations of the measured compounds in the two native species were compared with those in the English walnut (Juglans regia L.), a globally recognized cultivar, and its counterpart cultivated in Japan, Shinano walnut (Juglans regia L.). The results showed that Oni walnut and Hime walnut contained significantly higher protein and minerals and lower lipid content than conventional cultivars. However, both Oni and Hime walnuts contained higher ratios of unsaturated fatty acids in total fatty acids. This study offers novel insights into the nutritional components of Oni and Hime walnuts, contributing to a deeper understanding of their nutritional value and potential applications as unique native walnut species. The findings of this study highlight the relationship between the different types of walnut species and their nutritional composition, and the value of native walnuts used in local cuisine, and will lead to new developments in functional foods from walnut species consumed around the world. It will contribute to the development of functional and processed foods by increasing the production of native walnut species, which are rich in protein, unsaturated fatty acids and minerals and by using them in local cuisines and health-promoting foods.

  9. The Role of IgA in the Manifestation and Prevention of Allergic Immune Responses. International-journal Peer-reviewed

    Stephan Scheurer, Ann-Christine Junker, Chaoqi He, Stefan Schülke, Masako Toda

    Current allergy and asthma reports (国際共著論文)　2023/08/23

    DOI： 10.1007/s11882-023-01105-x 　

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    PURPOSE OF REVIEW: Immunoglobulin A (IgA) mediates immune exclusion of antigens in the gut. Notably, IgA plays also a role in the prevention of IgE-mediated allergies and induction of immune tolerance. The present review addresses the role of IgA in the manifestation of IgE-mediated allergies, including allergen-specific immunotherapy (AIT), the regulation of IgA production, and the mechanism of IgA in immune cell activation. RECENT FINDINGS: The majority of studies report an association of IgA with the induction of immune tolerance in IgE-mediated allergies. However, reports on the involvement of humoral and mucosal IgA, IgA subtypes, monomeric and polymeric IgA, and the mechanism of IgA-mediated immune cell activation are confounding. Effects by IgA are likely mediated by alteration of microbiota, IgE-blocking capacity, or activation of inhibitory signaling pathways. However, the precise mechanism of IgA-regulation, the contribution of serum and/or mucosal IgA, and IgA1/2 subtypes, on the manifestation of IgE-mediated allergies, and the underlying immune modulatory mechanism are still elusive.

  10. Advanced glycation end products and allergy

    Masako Toda, Michael Hellwig, Hiroyuki Hattori, Thomas Henle, Stefan Vieths

    Allergo Journal International (国際共著論文)　2023/06/28

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s40629-023-00259-4 　

    eISSN： 2197-0378

  11. Hypolipidemic and Anti-Inflammatory Effects of Curcuma longa-Derived Bisacurone in High-Fat Diet-Fed Mice. International-journal

    Chaoqi He, Taiki Miyazawa, Chizumi Abe, Takahiro Ueno, Mikiko Suzuki, Masashi Mizukami, Kazue Kurihara, Masako Toda

    International journal of molecular sciences　24　(11)　2023/05/27

    DOI： 10.3390/ijms24119366 　

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    Turmeric (Curcuma longa) contains various compounds that potentially improve health. Bisacurone is a turmeric-derived compound but has been less studied compared to other compounds, such as curcumin. In this study, we aimed to evaluate the anti-inflammatory and lipid-lowering effects of bisacurone in high-fat diet (HFD)-fed mice. Mice were fed HFD to induce lipidemia and orally administered bisacurone daily for two weeks. Bisacurone reduced liver weight, serum cholesterol and triglyceride levels, and blood viscosity in mice. Splenocytes from bisacurone-treated mice produced lower levels of the pro-inflammatory cytokines IL-6 and TNF-α upon stimulation with a toll-like receptor (TLR) 4 ligand, lipopolysaccharide (LPS), and TLR1/2 ligand, Pam3CSK4, than those from untreated mice. Bisacurone also inhibited LPS-induced IL-6 and TNF-α production in the murine macrophage cell line, RAW264.7. Western blot analysis revealed that bisacurone inhibited the phosphorylation of IKKα/β and NF-κB p65 subunit, but not of the mitogen-activated protein kinases, p38 kinase and p42/44 kinases, and c-Jun N-terminal kinase in the cells. Collectively, these results suggest that bisacurone has the potential to reduce serum lipid levels and blood viscosity in mice with high-fat diet-induced lipidemia and modulate inflammation via inhibition of NF-κB-mediated pathways.

  12. Quantitative Evaluation of Enzyme-Assisted Supercritical CO2 Extraction of Natural Essential Oil from Yuzu Peel and Evaluation for the Functionality of the Extraction Residue. Peer-reviewed

    Hiraga, Y, Kise, N, Torii, K, Toda, M, Sueyoshi, M, Ohashi, T, Watanabe, M

    Kagaku Kogaku Ronbunshu　49　(5)　133-141　2023/05

    DOI： 10.1252/kakoronbunshu.49.133 　

  13. The Impacts of Cholesterol, Oxysterols, and Cholesterol Lowering Dietary Compounds on the Immune System. International-journal Peer-reviewed

    Rintaro Yanagisawa, Chaoqi He, Akira Asai, Michael Hellwig, Thomas Henle, Masako Toda*

    International journal of molecular sciences (国際共著論文)　23　(20)　2022/10

    DOI： 10.3390/ijms232012236 　

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    Cholesterol and its oxidized forms, oxysterols, are ingested from foods and are synthesized de novo. Cholesterol and oxysterols influence molecular and cellular events and subsequent biological responses of immune cells. The amount of dietary cholesterol influence on the levels of LDL cholesterol and blood oxysterols plays a significant role in the induction of pro-inflammatory state in immune cells, leading to inflammatory disorders, including cardiovascular disease. Cholesterol and oxysterols synthesized de novo in immune cells and stroma cells are involved in immune homeostasis, which may also be influenced by an excess intake of dietary cholesterol. Dietary compounds such as β-glucan, plant sterols/stanols, omega-3 lipids, polyphenols, and soy proteins, could lower blood cholesterol levels by interfering with cholesterol absorption and metabolism. Such dietary compounds also have potential to exert immune modulation through diverse mechanisms. This review addresses current knowledge about the impact of dietary-derived and de novo synthesized cholesterol and oxysterols on the immune system. Possible immunomodulatory mechanisms elicited by cholesterol-lowering dietary compounds are also discussed.

  14. Food Antioxidants and Aging: Theory, Current Evidence and Perspectives

    Taiki Miyazawa, Chizumi Abe, Gregor Carpentero Burdeos, Akira Matsumoto, Masako Toda

    Nutraceuticals　2　181-204　2022/08

    Publisher: MDPI AG

    DOI： 10.3390/nutraceuticals2030014 　

    eISSN： 1661-3821

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    The concept of food and aging is of great concern to humans. So far, more than 300 theories of aging have been suggested, and approaches based on these principles have been investigated. It has been reported that antioxidants in foods might play a role in human aging. To clarify the current recognition and positioning of the relationship between these food antioxidants and aging, this review is presented in the following order: (1) aging theories, (2) food and aging, and (3) individual food antioxidants and aging. Clarifying the significance of food antioxidants in the field of aging will lead to the development of strategies to achieve healthy human aging.

  15. Artificial intelligence in food science and nutrition: a narrative review. International-journal

    Taiki Miyazawa, Yoichi Hiratsuka, Masako Toda, Nozomu Hatakeyama, Hitoshi Ozawa, Chizumi Abe, Ting-Yu Cheng, Yuji Matsushima, Yoshifumi Miyawaki, Kinya Ashida, Jun Iimura, Tomohiro Tsuda, Hiroto Bushita, Kazuichi Tomonobu, Satoshi Ohta, Hsuan Chung, Yusuke Omae, Takayuki Yamamoto, Makoto Morinaga, Hiroshi Ochi, Hajime Nakada, Kazuhiro Otsuka, Teruo Miyazawa

    Nutrition reviews　2022/05/30

    DOI： 10.1093/nutrit/nuac033 　

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    In the late 2010s, artificial intelligence (AI) technologies became complementary to the research areas of food science and nutrition. This review aims to summarize these technological advances by systematically describing the following: the use of AI in other fields (eg, engineering, pharmacy, and medicine); the history of AI in relation to food science and nutrition; the AI technologies currently used in the agricultural and food industries; and some of the important applications of AI in areas such as immunity-boosting foods, dietary assessment, gut microbiome profile analysis, and toxicity prediction of food ingredients. These applications are likely to be in great demand in the near future. This review can provide a starting point for brainstorming and for generating new AI applications in food science and nutrition that have yet to be imagined.

  16. Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice. International-journal Peer-reviewed

    Frank Blanco-Pérez, Irene Gonzalez-Menendez, Michael Stassen, Yoichiro Kato, Jonathan Laiño, Jörg Kirberg, Maren Krause, Manuela Martella, Noriyuki Shibata, Leticia Quintanilla-Martinez, Thorsten B Feyerabend, Hans-Reimer Rodewald, Stephen J Galli, Stefan Vieths, Stephan Scheurer, Masako Toda*

    Allergy (国際共著論文)　77　(3)　1051　2022

    DOI： 10.1111/all.15182 　

  17. Differences in allergic symptoms after the consumption of egg yolk and egg white. International-journal Peer-reviewed

    Kei Uneoka, Satoshi Horino, Ayafumi Ozaki, Haruka Aki, Masako Toda, Katsushi Miura

    Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology　17　(1)　97-97　2021

    DOI： 10.1186/s13223-021-00599-2 　

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    Hen's eggs are one of the most common causes of food allergy. Although hen's eggs are known to cause more gastrointestinal symptoms than other foods, it is not known whether there is a difference in organ-specific symptoms between egg yolk (EY) and egg white (EW). The present study aimed to determine whether there are organ-specific differences in the immediate symptoms of EY and EW in patients with hen's egg allergies. We retrospectively investigated the immediate symptoms and treatment contents of those who had a positive result in an oral food challenge (OFC) of boiled whole EY or 10 g of boiled EW in our hospital from January 2013 to July 2019. We compared 80 patients in the EY-OFC-positive group with 106 patients in the EW-OFC-positive group. The EY-OFC-positive group had significantly fewer respiratory symptoms and significantly more gastrointestinal symptoms than the EW-OFC-positive group and had significantly more gastrointestinal symptoms only. In terms of treatment, significantly fewer patients in the EY-OFC-positive group required beta 2-agonist inhalation, and a significantly higher proportion of patients did not require treatment. Compared to EW, EY is more likely to cause gastrointestinal symptoms and less likely to cause respiratory symptoms. It may be necessary to discriminate between EY and EW allergy during diagnosis.

  18. The Dietary Fiber Pectin: Health Benefits and Potential for the Treatment of Allergies by Modulation of Gut Microbiota. International-journal Peer-reviewed

    Frank Blanco-Pérez, Hanna Steigerwald, Stefan Schülke, Stefan Vieths, Masako Toda, Stephan Scheurer

    Current allergy and asthma reports (国際共著論文)　21　(10)　43-43　2021

    DOI： 10.1007/s11882-021-01020-z 　

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    PURPOSE OF REVIEW: The incidence of allergies is increasing and has been associated with several environmental factors including westernized diets. Changes in environment and nutrition can result in dysbiosis of the skin, gut, and lung microbiota altering the production of microbial metabolites, which may in turn generate epigenetic modifications. The present review addresses studies on pectin-mediated effects on allergies, including the immune modulating mechanisms by bacterial metabolites. RECENT FINDINGS: Recently, microbiota have gained attention as target for allergy intervention, especially with prebiotics, that are able to stimulate the growth and activity of certain microorganisms. Dietary fibers, which cannot be digested in the gastrointestinal tract, can alter the gut microbiota and lead to increased local and systemic concentrations of gut microbiota-derived short chain fatty acids (SCFAs). These can promote the generation of peripheral regulatory T cells (Treg) by epigenetic modulation and suppress the inflammatory function of dendritic cells (DCs) by transcriptional modulation. The dietary fiber pectin (a plant-derived polysaccharide commonly used as gelling agent and dietary supplement) can alter the ratio of Firmicutes to Bacteroidetes in gut and lung microbiota, increasing the concentrations of SCFAs in feces and sera, and reducing the development of airway inflammation by suppressing DC function. Pectin has shown immunomodulatory effects on allergies, although the underlying mechanisms still need to be elucidated. It has been suggested that the different types of pectin may exert direct and/or indirect immunomodulatory effects through different mechanisms. However, little is known about the relation of certain pectin structures to allergies.

  19. β-(1→4)-mannobiose acts as an immunostimulatory molecule in murine dendritic cells by binding the TLR4/MD-2 complex International-journal Peer-reviewed

    Ting-Yu Cheng, Yen-Ju Lin, Wataru Saburi, Stefan Vieths, Stephan Scheurer, Stefan Schülke, Masako Toda

    Cells (国際共著論文)　10　(7)　1774　2021

    DOI： 10.3390/cells10071774 　

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    Some β-mannans, including those in coffee bean and soy, contain a mannose backbone with β-(1→4) bonds. Such mannooligosaccharides could have immunological functions involving direct interaction with immune cells, in addition to acting as prebiotics. This study aimed at assessing the immunological function of mannooligosaccharides with β-(1→4) bond, and elucidating their mechanism of action using bone marrow-derived murine dendritic cells (BMDCs). When BMDCs were stimulated with the mannooligosaccharides, only β-Man-(1→4)-Man significantly induced production of cytokines that included IL-6, IL-10, TNF-α, and IFN-β, and enhanced CD4+ T-cell stimulatory capacity. Use of putative receptor inhibitors revealed the binding of β-Man-(1→4)-Man to TLR4/MD2 complex and involvement with the complement C3a receptor (C3aR) for BMDC activation. Interestingly, β-Man-(1→4)-Man prolonged the production of pro-inflammatory cytokines (IL-6 and TNF-α), but not of the IL-10 anti-inflammatory cytokine during extended culture of BMDCs, associated with high glucose consumption. The results suggest that β-Man-(1→4)-Man is an immunostimulatory molecule, and that the promotion of glycolysis could be involved in the production of pro-inflammatory cytokine in β-Man-(1→4)-Man-stimulated BMDCs. This study could contribute to development of immune-boosting functional foods and a novel vaccine adjuvant.

  20. Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms. International-journal Peer-reviewed

    Maren Krause, Peter Crauwels, Frank Blanco-Pérez, Martin Globisch, Andrea Wangorsch, Thomas Henle, Jonas Lidholm, Ger van Zandbergen, Stefan Vieths, Stephan Scheurer, Masako Toda

    Scientific Reports（国際共著論文）　11　(1)　10141-10141　2021

    DOI： 10.1038/s41598-021-89402-1 　

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    Evidence has suggested that major peanut allergen Ara h 1 activates dendritic cells (DCs) via interaction with DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin), a C-type lectin receptor, and contributes to development of peanut allergy. Since macrophages, as well as DCs, play a crucial role in innate immunity, we investigated whether natural Ara h 1 (nAra h 1) activates two different subsets of macrophages, human monocyte derived macrophage type 1 (hMDM1: pro-inflammatory model) and type 2 (hMDM2: anti-inflammatory model). hMDM1 and hMDM2 predominantly produced pro-inflammatory cytokines (IL-6 and TNF-α) and an anti-inflammatory cytokine (IL-10) in response to nAra h 1, respectively. hMDM2 took up nAra h 1 and expressed DC-SIGN at higher levels than hMDM1. However, small interfering RNA knockdown of DC-SIGN did not suppress nAra h 1 uptake and nAra h 1-mediated cytokine production in hMDM2. Inhibitors of scavenger receptor class A type I (SR-AI) suppressed the response of hMDM2, but not of hMDM1, suggesting that SR-AI is a major receptor in hMDM2 for nAra h 1 recognition and internalization. nAra h 1 appears to exert stimulatory capacity on DC and macrophages via different receptors. This study advances our understanding how a major peanut allergen interacts with innate immunity.

  21. Macadamia nut allergy in children: clinical features and cross-reactivity with walnut. International-journal Peer-reviewed

    Koichi Yoshida, Shoichiro Shirane, Kazue Kinoshita, Emi Morikawa, Shoko Matsushita, Masako Toda, Haruyo Nakajima-Adachi, Akira Akasawa, Masami Narita

    Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology　32　(5)　1111-1114　2021

    DOI： 10.1111/pai.13469 　

    More details Close

    Macadamia nuts, the seeds of Macadamia tetraphylla or Macadamia integrifolia of family Proteaceae, are a familiar food item. Previous case reports of macadamia nut allergy demonstrated the possibility of false negative IgE antibody test results and cross-reactivity of macadamia nut with hazelnut.1-5 However, the clinical features of childhood macadamia nut allergy and the cross-reactivity of macadamia nut with foods other than hazelnut remain understudied.

  22. Identification and Characterization of IgE-Reactive Proteins and a New Allergen (Cic a 1.01) from Chickpea (Cicer arietinum). International-journal Peer-reviewed

    Andrea Wangorsch, Anuja Kulkarni, Annette Jamin, Jelena Spiric, Julia Bräcker, Jens Brockmeyer, Vera Mahler, Natalia Blanca-López, Marta Ferrer, Miguel Blanca, Maria Torres, Paqui Gomez, Joan Bartra, Alba García-Moral, María J Goikoetxea, Stefan Vieths, Masako Toda, Gianni Zoccatelli, Stephan Scheurer

    Molecular nutrition & food research （国際共著論文）　64　(19)　e2000560　2020/10

    DOI： 10.1002/mnfr.202000560 　

    More details Close

    SCOPE: Chickpea (Cicer arietinum) allergy has frequently been reported particularly in Spain and India. Nevertheless, chickpea allergens are poorly characterized. The authors aim to identify and characterize potential allergens from chickpea. METHODS AND RESULTS: Candidate proteins are selected by an in silico approach or immunoglobuline E (IgE)-testing. Potential allergens are prepared as recombinant or natural proteins and characterized for structural integrity by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), circular dichroism (CD)-spectroscopy, and mass spectrometry (MS) analysis. IgE-sensitization pattern of Spanish chickpea allergic and German peanut and birch pollen sensitized patients are investigated using chickpea extracts and purified proteins. Chickpea allergic patients show individual and heterogeneous IgE-sensitization profiles with extracts from raw and boiled chickpeas. Chickpea proteins pathogenesis related protein family 10 (PR-10), a late embryogenesis abundant protein (LEA/DC-8), and a vicilin-containing fraction, but not 2S albumin, shows IgE reactivity with sera from chickpea, birch pollen, and peanut sensitized patients. Remarkably, allergenic vicilin, DC-8, and PR-10 are detected in the extract of boiled chickpeas. CONCLUSION: Several IgE-reactive chickpea allergens are identified. For the first time a yet not classified DC-8 protein is characterized as minor allergen (Cic a 1). Finally, the data suggest a potential risk for peanut allergic patients by IgE cross-reactivity with homologous chickpea proteins.

  23. Interference with SAMHD1 restores late gene expression of modified vaccinia virus Ankara (MVA) in human dendritic cells and abrogates type I interferon expression. Peer-reviewed

    Sliva K, Martin J, von Rhein C, Herrmann T, Weyrich A, Toda M, Schnierle BS

    Journal of virology　2019/08

    DOI： 10.1128/JVI.01097-19 　

    ISSN： 0022-538X

  24. CCR8 leads to eosinophil migration and regulates neutrophil migration in murine allergic enteritis. Peer-reviewed

    Blanco-Pérez F, Kato Y, Gonzalez-Menendez I, Laiño J, Ohbayashi M, Burggraf M, Krause M, Kirberg J, Iwakura Y, Martella M, Quintanilla-Martinez L, Shibata N, Vieths S, Scheurer S, Toda M

    Scientific reports　9　(1)　9608　2019/07

    DOI： 10.1038/s41598-019-45653-7 　

  25. Influence of the Maillard Reaction on the Allergenicity of Food Proteins and the Development of Allergic Inflammation. Peer-reviewed

    Toda M, Hellwig M, Henle T, Vieths S

    Current allergy and asthma reports　19　(1)　4　2019/01

    DOI： 10.1007/s11882-019-0834-x 　

    ISSN： 1529-7322

  26. Rice Components with Immunomodulatory Function. Peer-reviewed

    Toda M

    Journal of nutritional science and vitaminology　65　(Supplement)　S9-S12　2019

    DOI： 10.3177/jnsv.65.S9 　

    ISSN： 0301-4800

  27. Critical role of mammalian target of rapamycin for IL-10 dendritic cell induction by a flagellin A conjugate in preventing allergic sensitization. Peer-reviewed

    Schülke S, Fiedler AH, Junker AC, Flaczyk A, Wolfheimer S, Wangorsch A, Heinz A, Beckert H, Nagl B, Bohle B, Vieths S, Toda M, Scheurer S

    The Journal of allergy and clinical immunology　141　(5)　1786-1798.e11　2018/05

    DOI： 10.1016/j.jaci.2017.07.002 　

    ISSN： 0091-6749

  28. Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice. Peer-reviewed

    Rodriguez MJ, Wangorsch A, Gomez F, Schülke S, Torres MJ, Vieths S, Scheurer S, Toda M, Mayorga C, Equal contribution

    Journal of immunology research　2018　3479185　2018

    DOI： 10.1155/2018/3479185 　

    ISSN： 2314-8861

  29. Conjugation of wildtype and hypoallergenic mugwort allergen Art v 1 to flagellin induces IL-10-DC and suppresses allergen-specific TH2-responses in vivo Peer-reviewed

    Schuelke Stefan, Kuttich Kirsten, Wolfheimer Sonja, Duschek Nadine, Wangorsch Andrea, Reuter Andreas, Briza Peter, Pablos Isabel, Gadermaier Gabriele, Ferreira Fatima, Vieths Stefan, Toda Masako, Scheurer Stephan

    SCIENTIFIC REPORTS　7　(1)　11782　2017/09

    DOI： 10.1038/s41598-017-11972-w 　

    ISSN： 2045-2322

  30. Cell-permeable capsids as universal antigen carrier for the induction of an antigen-specific CD8(+) T-cell response Peer-reviewed

    Sami Akhras, Masako Toda, Klaus Boller, Kiyoshi Himmelsbach, Fabian Elgner, Marlene Biehl, Stephan Scheurer, Meike Gratz, Stefan Vieths, Eberhard Hildt

    SCIENTIFIC REPORTS　7　(1)　9630　2017/08

    DOI： 10.1038/s41598-017-08787-0 　

    ISSN： 2045-2322

  31. Targeting of Immune Cells by Dual TLR2/7 Ligands Suppresses Features of Allergic Th2 Immune Responses in Mice Peer-reviewed

    Jonathan Laino, Andrea Wangorsch, Frank Blanco, Sonja Wolfheimer, Maren Krause, Adam Flaczyk, Tobias-Maximilian Moeller, Mindy Tsai, Stephen Galli, Stefan Vieths, Masako Toda, Stephan Scheurer, Stefan Schuelke

    JOURNAL OF IMMUNOLOGY RESEARCH　2017　7983217　2017

    DOI： 10.1155/2017/7983217 　

    ISSN： 2314-8861

    eISSN： 2314-7156

  32. Epicutaneous immunotherapy with a hypoallergenic Bet v 1 suppresses allergic asthma in a murine model Peer-reviewed

    S. Siebeneicher, S. Reuter, A. Wangorsch, M. Krause, K. Foetisch, A. Heinz, S. Naito, A. Reuter, C. Taube, S. Vieths, S. Scheurer, M. Toda

    ALLERGY　70　(12)　1559-1568　2015/12

    DOI： 10.1111/all.12732 　

    ISSN： 0105-4538

    eISSN： 1398-9995

  33. What makes an allergen? Peer-reviewed

    S. Scheurer, M. Toda, S. Vieths

    CLINICAL AND EXPERIMENTAL ALLERGY　45　(7)　1150-1161　2015/07

    DOI： 10.1111/cea.12571 　

    ISSN： 0954-7894

    eISSN： 1365-2222

  34. Peyer's Patches and Mesenteric Lymph Nodes Cooperatively Promote Enteropathy in a Mouse Model of Food Allergy Peer-reviewed

    Haruyo Nakajima-Adachi, Akira Kikuchi, Yoko Fujimura, Kyoko Shibahara, Tsuyoshi Makino, Masae Goseki-Sone, Miran Kihara-Fujioka, Tomonori Nochi, Yosuke Kurashima, Osamu Igarashi, Masafumi Yamamoto, Jun Kunisawa, Masako Toda, Shuichi Kaminogawa, Ryuichiro Sato, Hiroshi Kiyono, Satoshi Hachimura

    PLOS ONE　9　(10)　e107492　2014/10

    DOI： 10.1371/journal.pone.0107492 　

    ISSN： 1932-6203

  35. Epicutaneous immune modulation with Bet v 1 plus R848 suppresses allergic asthma in a murine model Peer-reviewed

    S. Siebeneicher, S. Reuter, M. Krause, A. Wangorsch, J. Maxeiner, S. Wolfheimer, S. Schuelke, S. Naito, A. Heinz, C. Taube, S. Vieths, S. Scheurer, M. Toda

    ALLERGY　69　(3)　328-337　2014/03

    DOI： 10.1111/all.12326 　

    ISSN： 0105-4538

    eISSN： 1398-9995

  36. Ovalbumin Modified with Pyrraline, a Maillard Reaction Product, shows Enhanced T-cell Immunogenicity Peer-reviewed

    Monika Heilmann, Anne Wellner, Gabriele Gadermaier, Anne Ilchmann, Peter Briza, Maren Krause, Ryoji Nagai, Sven Burgdorf, Stephan Scheurer, Stefan Vieths, Thomas Henle, Masako Toda

    JOURNAL OF BIOLOGICAL CHEMISTRY　289　(11)　7919-7928　2014/03

    DOI： 10.1074/jbc.M113.523621 　

    ISSN： 0021-9258

    eISSN： 1083-351X

  37. Heat treatment of egg white controls allergic symptoms and induces oral tolerance to ovalbumin in a murine model of food allergy Peer-reviewed

    Hiroko Watanabe, Masako Toda, Haruko Sekido, Anne Wellner, Tomoyuki Fujii, Thomas Henle, Satoshi Hachimura, Haruyo Nakajima-Adachi

    MOLECULAR NUTRITION & FOOD RESEARCH　58　(2)　394-404　2014/02

    DOI： 10.1002/mnfr.201300205 　

    ISSN： 1613-4125

    eISSN： 1613-4133

  38. Prevention of Intestinal Allergy in Mice by rflaA: Ova Is Associated with Enforced Antigen Processing and TLR5-Dependent IL-10 Secretion by mDC Peer-reviewed

    Stefan Schuelke, Sonja Wolfheimer, Gabriele Gadermaier, Andrea Wangorsch, Susanne Siebeneicher, Peter Briza, Ingo Spreitzer, Dirk Schiller, Bettina Loeschner, Satoshi Uematsu, Bernard Ryffel, Shizuo Akira, Zoe Waibler, Stefan Vieths, Masako Toda, Stephan Scheurer

    PLOS ONE　9　(2)　e87822　2014/02

    DOI： 10.1371/journal.pone.0087822 　

    ISSN： 1932-6203

  39. The Maillard reaction and food allergies: is there a link? Peer-reviewed

    Masako Toda, Monika Heilmann, Anne Ilchmann, Stefan Vieths

    CLINICAL CHEMISTRY AND LABORATORY MEDICINE　52　(1)　61-67　2014/01

    DOI： 10.1515/cclm-2012-0830 　

    ISSN： 1434-6621

    eISSN： 1437-4331

  40. Evidence That Formation of Vimentin.Mitogen-activated Protein Kinase (MAPK) Complex Mediates Mast Cell Activation following Fc epsilon RI/CC Chemokine Receptor 1 Cross-talk Peer-reviewed

    Masako Toda, Chuan-Hui Kuo, Satty K. Borman, Ricardo Micheler Richardson, Akihito Inoko, Masaki Inagaki, Andrea Collins, Klaus Schneider, Santa Jeremy Ono

    JOURNAL OF BIOLOGICAL CHEMISTRY　287　(29)　24516-24524　2012/07

    DOI： 10.1074/jbc.M111.319624 　

    ISSN： 0021-9258

  41. Identification of genes and proteins specifically regulated by costimulation of mast cell Fc epsilon Receptor I and chemokine receptor 1 Peer-reviewed

    Cho Cho Aye, Masako Toda, Kei Morohoshi, Santa J. Ono

    EXPERIMENTAL AND MOLECULAR PATHOLOGY　92　(3)　267-274　2012/06

    DOI： 10.1016/j.yexmp.2012.02.002 　

    ISSN： 0014-4800

  42. Impact of culture medium on maturation of bone marrow-derived murine dendritic cells via the aryl hydrocarbon receptor Peer-reviewed

    Anne Ilchmann, Maren Krause, Monika Hellmann, Sven Burgdorf, Stefan Vieths, Masako Toda

    MOLECULAR IMMUNOLOGY　51　(1)　42-50　2012/05

    DOI： 10.1016/j.molimm.2012.02.005 　

    ISSN： 0161-5890

  43. A fusion protein of flagellin and ovalbumin suppresses the T(H)2 response and prevents murine intestinal allergy Peer-reviewed

    Stefan Schuelke, Manja Burggraf, Zoe Waibler, Andrea Wangorsch, Sonja Wolfheimer, Ulrich Kalinke, Stefan Vieths, Masako Toda, Stephan Scheurer

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　128　(6)　1340-U728　2011/12

    DOI： 10.1016/j.jaci.2011.07.036 　

    ISSN： 0091-6749

  44. Protein unfolding strongly modulates the allergenicity and immunogenicity of Pru p 3, the major peach allergen Peer-reviewed

    Masako Toda, Gerald Reese, Gabriele Gadermaier, Veronique Schulten, Iris Lauer, Matthias Egger, Peter Briza, Stefanie Randow, Sonja Wolfheimer, Valencia Kigongo, Maria del Mar San Miguel Moncin, Kay Foetisch, Barbara Bohle, Stefan Vieths, Stephan Scheurer

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　128　(5)　1022-U470　2011/11

    DOI： 10.1016/j.jaci.2011.04.020 　

    ISSN： 0091-6749

  45. Oral tolerance induction does not resolve gastrointestinal inflammation in a mouse model of food allergy Peer-reviewed

    Manja Burggraf, Haruyo Nakajima-Adachi, Satoshi Hachimura, Anne Ilchmann, Alan D. Pemberton, Hiroshi Kiyono, Stefan Vieths, Masako Toda

    MOLECULAR NUTRITION & FOOD RESEARCH　55　(10)　1475-1483　2011/10

    DOI： 10.1002/mnfr.201000634 　

    ISSN： 1613-4125

  46. Approaches to immunotherapies for Japanese cedar pollinosis Peer-reviewed

    Masahiro Sakaguchi, Kazulci Hirahara, Takashi Fujimura, Masako Toda

    AURIS NASUS LARYNX　38　(4)　431-438　2011/08

    DOI： 10.1016/j.anl.2010.12.002 　

    ISSN： 0385-8146

    eISSN： 1879-1476

  47. Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide Peer-reviewed

    Y. Kaburaki, T. Fujimura, K. Kurata, K. Masuda, M. Toda, H. Yasueda, K. Chida, S. Kawarai, M. Sakaguchi

    COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES　34　(2)　157-161　2011/03

    DOI： 10.1016/j.cimid.2010.06.005 　

    ISSN： 0147-9571

  48. Fusion protein of TLR5-ligand and allergen potentiates activation and IL-10 secretion in murine myeloid DC Peer-reviewed

    Stefan Schulke, Zoe Waibler, Marc-Stefan Mende, Gianni Zoccatelli, Stefan Vieths, Masako Toda, Stephan Scheurer

    MOLECULAR IMMUNOLOGY　48　(1-3)　341-350　2010/11

    DOI： 10.1016/j.molimm.2010.07.006 　

    ISSN： 0161-5890

  49. Effects of glycation of the model food allergen ovalbumin on antigen uptake and presentation by human dendritic cells Peer-reviewed

    Tamara Hilmenyuk, Iris Bellinghausen, Baerbel Heydenreich, Anne Ilchmann, Masako Toda, Stephan Grabbe, Joachim Saloga

    IMMUNOLOGY　129　(3)　437-445　2010/03

    DOI： 10.1111/j.1365-2567.2009.03199.x 　

    ISSN： 0019-2805

  50. Glycation of a food allergen by the Maillard reaction enhances its T-cell immunogenicity: Role of macrophage scavenger receptor class A type I and II Peer-reviewed

    Anne Ilchmann, Sven Burgdorf, Stephan Scheurer, Zoe Waibler, Ryoji Nagai, Anne Wellner, Yasuhiko Yamamoto, Hiroshi Yamamoto, Thomas Henle, Christian Kurts, Ulrich Kalinke, Stefan Vieths, Masako Toda

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　125　(1)　175-183　2010/01

    DOI： 10.1016/j.jaci.2009.08.013 　

    ISSN： 0091-6749

  51. Macular and retinal dysfunction of unknown origin in adults with normal fundi: Evidence for an autoimmune pathophysiology Peer-reviewed

    Irmela Mantel, Kanchan V. Ramchand, Graham E. Holder, Masaharu Ohbayashi, Kei Morohoshi, Nishal Patel, Masako Toda, Fred W. Fitzke, Alan C. Bird, Santa Jeremy Ono

    EXPERIMENTAL AND MOLECULAR PATHOLOGY　84　(2)　90-101　2008/04

    DOI： 10.1016/j.yexmp.2007.10.006 　

    ISSN： 0014-4800

    eISSN： 1096-0945

  52. Dynamic changes in conjunctival dendritic cell numbers, anatomical position and phenotype during experimental allergic conjunctivitis Peer-reviewed

    Masaharu Ohbayashl, Bita Manzouri, Tom Flynn, Masako Toda, Yoshifurni Ikeda, Takao Nakamura, Santa Jeremy Ono

    EXPERIMENTAL AND MOLECULAR PATHOLOGY　83　(2)　216-223　2007/10

    DOI： 10.1016/j.yexmp.2007.04.007 　

    ISSN： 0014-4800

  53. Role of beta-chemokines in mast cell activation and type I hypersensitivity reactions in the conjunctiva: in vivo and in vitro studies Peer-reviewed

    Frederick Beer, Chuan-Hui Kuo, Kei Morohoshi, James Goodliffe, Peter Munro, Cho Cho Aye, Maria Dawson, Ricardo M. Richardson, Limei H. Jones, Yoshifumi Ikeda, Takao Nakamura, Masako Toda, Tom Flynn, Masaharu Ohbayashi, Dai Miyazaki, Santa Jeremy Ono

    IMMUNOLOGICAL REVIEWS　217　96-104　2007/06

    DOI： 10.1111/j.1600-065X.2007.00521.x 　

    ISSN： 0105-2896

  54. Mechanisms of leukocyte trafficking in allergic diseases: Insights into new therapies targeting chemokines and chemokine receptors Peer-reviewed

    Masako Toda, Takao Nakamura, Masaharu Ohbayashi, Yoshifumi Ikeda, Maria Dawson, Cho Cho Aye, Dai Miyazaki, Santa Jeremy Ono

    Expert Review of Clinical Immunology　3　(3)　351-364　2007/05

    DOI： 10.1586/1744666X.3.3.351 　

    ISSN： 1744-666X

  55. A specific CCR3 chemokine receptor antagonist inhibits both early and late phase allergic inflammation in the conjunctiva Peer-reviewed

    Takao Nakamura, Masaharu Ohbayashi, Masako Toda, David A. Hall, Carmel M. Horgan, Santa Jeremy Ono

    IMMUNOLOGIC RESEARCH　33　(3)　213-221　2005/12

    DOI： 10.1385/IR:33:3:213 　

    ISSN： 0257-277X

  56. Circulating anti-retinal antibodies as immune markers in age-related macular degeneration Peer-reviewed

    N Patel, M Ohbayashi, AK Nugent, K Ramchand, M Toda, KY Chau, C Bunce, A Webster, AC Bird, SJ Ono, Chong, V

    IMMUNOLOGY　115　(3)　422-430　2005/07

    DOI： 10.1111/j.1365-2567.2005.02173.x 　

    ISSN： 0019-2805

  57. Macrophage inflammatory protein-1 alpha as a costimulatory signal for mast cell-mediated immediate hypersensitivity reactions Peer-reviewed

    D Miyazaki, T Nakamura, M Toda, KW Cheung-Chau, RM Richardson, SJ Ono

    JOURNAL OF CLINICAL INVESTIGATION　115　(2)　434-442　2005/02

    DOI： 10.1172/JCI200518452 　

    ISSN： 0021-9738

  58. Role of CC chemokines and their receptors in multiple aspects of mast cell biology: Comparative protein profiling of FcεRI- and/or CCR1-engaged mast cells using protein chip technology Peer-reviewed

    Masako Toda, Takao Nakamura, Masaharu Ohbayashi, Yoshifumi Ikeda, Maria Dawson, Ricardo Micheler Richardson, Andrew Alban, Benjamin Leed, Dai Miyazaki, Santa Jeremy Ono

    Novartis Foundation Symposium　271　131-140　2005

    ISSN： 1528-2511

  59. Impact of engagement of Fc delta RI and CC chemokine receptor 1 on mast cell activation and motility Peer-reviewed

    M Toda, M Dawson, T Nakamura, PMG Munro, RM Richardson, M Bailly, SJ Ono

    JOURNAL OF BIOLOGICAL CHEMISTRY　279　(46)　48443-48448　2004/11

    DOI： 10.1074/jbc.M408725200 　

    ISSN： 0021-9258

  60. Dermal dendritic cells sensitized with plasmid DNA encoding immunostimulatory sequence by gene gun efficiently prime murine HIV-1-specific CD8+ cytotoxic T lymphocytes Peer-reviewed

    Chizuno Hidaka, Yoshihiko Norose, Yohko Nakagawa, Masumi Shimizu, Megumi Takahashi, Atsuko Ohwaki, Kyoko Nohtomi, Masako Toda, Shigeru Kusagawa, Masahiro Sakaguchi, Shoji Kudo, Yutaka Takebe, Hidemi Takahashi

    Biomedical Research　25　(2)　83-91　2004/04

    DOI： 10.2220/biomedres.25.83 　

    ISSN： 0388-6107 1880-313X

  61. Signaling-cross talk between Fc(epsilon)RI and CC chemokine receptor 1 in mast cells Peer-reviewed

    M Toda, T Nakamura, RM Richardson, SJ Ono

    IMMUNOLOGY 2004: CYTOKINE NETWORK, REGULATORY CELLS, SIGNALING, AND APOPTOSIS　517-520　2004

  62. CAT-213, a specific monoclonal anti-eotaxin-1 human antibody, inhibits human conjunctival mast cell activation Peer-reviewed

    T Nakamura, F Larkin, Y Ikeda, M Ohbayashi, M Toda, MR Wilkins, JK Dart, IK Anderson, SJ Ono

    IMMUNOLOGY 2004: GENOMIC ISSUES, IMMUNE SYSTEM ACTIVATION AND ALLERGY　527-530　2004

  63. Expression profiling: Opportunities and pitfalls and impact on the study and management of allergic diseases Peer-reviewed

    SJ Ono, T Nakamura, M Ohbayashi, M Dawson, Y Ikeda, AK Nugent, M Toda, G Jay

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　112　(6)　1050-1056　2003/12

    DOI： 10.1016/j.jaci.2003.09.022 　

    ISSN： 0091-6749

  64. Detailed criteria for the assessment of clinical symptoms in a new murine model of severe allergic conjunctivitis Peer-reviewed

    T Nakamura, M Toda, M Ohbayashi, SJ Ono

    CORNEA　22　(7)　S13-S18　2003/10

    ISSN： 0277-3740

  65. Chemokines: Roles in leukocyte development, trafficking, and effector function Peer-reviewed

    SJ Ono, T Nakamura, D Miyazaki, M Ohbayashi, M Dawson, M Toda

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　111　(6)　1185-1199　2003/06

    DOI： 10.1067/mai.2003.1594 　

    ISSN： 0091-6749

  66. Analysis of sequential immunoglobulin E-binding epitope of Japanese cedar pollen allergen (Cry j 2) in humans, monkeys and mice Peer-reviewed

    Y Tamura, J Kawaguchi, N Serizawa, K Hirahara, A Shiraishi, H Nigi, Y Taniguchi, M Toda, S Inouye, T Takemori, M Sakaguchi

    CLINICAL AND EXPERIMENTAL ALLERGY　33　(2)　211-217　2003/02

    DOI： 10.1046/j.1365-2222.2003.01579.x 　

    ISSN： 0954-7894

  67. Genomics and proteomics of allergic disease Peer-reviewed

    M Toda, SJ Ono

    IMMUNOLOGY　106　(1)　1-10　2002/05

    DOI： 10.1046/j.1365-2567.2002.01407.x 　

    ISSN： 0019-2805

  68. DNA vaccine using invariant chain gene for delivery of CD4+T cell epitope peptide derived from Japanese cedar pollen allergen inhibits allergen-specific IgE response Peer-reviewed

    Toda, M., Kasai, M., Hosokawa, H., Nakano, N., Taniguchi, Y., Inouye, S., Kaminogawa, S., Takemori, T., Sakaguchi, M.

    European Journal of Immunology　32　(6)　1631-1639　2002

    DOI： 10.1002/1521-4141(200206)32:6<1631::AID-IMMU1631>3.0.CO;2-O 　

    ISSN： 0014-2980

  69. Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies Peer-reviewed

    M Sakaguchi, K Masuda, M Toda, S Inouye, H Yasueda, Y Taniguchi, T Nagoya, DJ DeBoer, H Tsujimoto

    VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY　78　(1)　35-43　2001/01

    DOI： 10.1016/S0165-2427(00)00250-6 　

    ISSN： 0165-2427

  70. Identification of a sequential B-cell epitope on major allergen (Cry j 1) of Japanese cedar (Cryptomeria japonica) pollen in mice Peer-reviewed

    Y Tamura, R Sasaki, S Inouye, J Kawaguchi, N Serizawa, M Toda, T Takemori, M Sakaguchi

    INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY　123　(3)　228-235　2000/11

    DOI： 10.1159/000024448 　

    ISSN： 1018-2438

  71. Minimum estimated incidence in Japan of anaphylaxis to live virus vaccines including gelatin Peer-reviewed

    M Sakaguchi, T Nakayama, H Fujita, M Toda, S Inouye

    VACCINE　19　(4-5)　431-436　2000/10

    DOI： 10.1016/S0264-410X(00)00206-1 　

    ISSN： 0264-410X

  72. IgE antibody to fish gelatin (type I collagen) in patients with fish allergy Peer-reviewed

    M Sakaguchi, M Toda, T Ebihara, S Irie, H Hori, A Imai, M Yanagida, H Miyazawa, H Ohsuna, Z Ikezawa, S Inouye

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　106　(3)　579-584　2000/09

    DOI： 10.1067/mai.2000.108499 　

    ISSN： 0091-6749

  73. Down-regulation of antigen-specific antibody production by TCR antagonist peptides in vivo Peer-reviewed

    M Toda, M Totsuka, S Furukawa, K Yokota, T Yoshioka, A Ametani, S Kaminogawa

    EUROPEAN JOURNAL OF IMMUNOLOGY　30　(2)　403-414　2000/02

    DOI： 10.1002/1521-4141(200002)30:2<403::AID-IMMU403>3.0.CO;2-8 　

    ISSN： 0014-2980

  74. Inhibition of immunoglobulin E response to Japanese cedar pollen allergen (Cry j 1) in mice by DNA immunization: different outcomes dependent on the plasmid DNA inoculation method Peer-reviewed

    M Toda, H Sato, Y Takebe, T Taniguchi, S Saito, S Inouye, T Takemori, M Sakaguchi

    IMMUNOLOGY　99　(2)　179-186　2000/02

    DOI： 10.1046/j.1365-2567.2000.00935.x 　

    ISSN： 0019-2805

  75. IgE reactivity to alpha 1 and alpha 2 chains of bovine type I collagen in children with bovine gelatin allergy Peer-reviewed

    M Sakaguchi, H Hori, S Hattori, S Irie, A Imai, M Yanagida, H Miyazawa, M Toda, S Inouye

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　104　(3)　695-699　1999/09

    DOI： 10.1016/S0091-6749(99)70344-1 　

    ISSN： 0091-6749

  76. Differential effect of antigen analogs with T cell inhibitory activity on in vivo antibody response Peer-reviewed

    M Toda, M Totsuka, T Yoshioka, A Ametani, S Kaminogawa

    ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 9　9　91-95　1998

  77. Antigen-specific inhibition of immune responses by antigen analogs with a single amino acid substitution Peer-reviewed

    M Totsuka, S Furukawa, M Toda, A Ametani, S Kaminogawa

    ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 8　8　505-509　1997

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Misc. 15

  1. 研究者の流動性について

    戸田雅子

    化学と生物　61　(11)　521　2023

  2. Epicutaneous immunotherapy with a hypoallergenic Bet v 1 suppresses asthmatic features in a murine model of birch pollen allergy

    S. Siebeneicher, S. Reuter, A. Wangorsch, M. Krause, K. Foetisch, A. Heinz, S. Naito, A. Reuter, C. Taube, S. Vieths, S. Scheurer, M. Toda

    ALLERGY　71　230-230　2016/08

    ISSN： 0105-4538

    eISSN： 1398-9995

  3. Bet v 1-homologous proteins from chickpea show IgE cross reactivity in patients with birch pollen allergy

    A. Kulkarni, A. Wangorsch, N. Blanca-Lopez, M. Ferrer, L. Ananthanarayan, S. Vieths, M. Toda, S. Scheurer

    ALLERGY　71　459-459　2016/08

    ISSN： 0105-4538

    eISSN： 1398-9995

  4. Induction of tolerogenic IL-10 from mouse dendritic cells by a fusion protein of TLR5-ligand flagellin and the major mugwort allergen Art v 1

    K. Kuttich, S. Schuelke, A. Wangorsch, A-C Junker, S. Wolfheimer, Pablos, I, G. Gadermaier, F. Ferreira-Briza, S. Vieths, M. Toda, S. Scheurer

    ALLERGY　70　38-39　2015/09

    ISSN： 0105-4538

    eISSN： 1398-9995

  5. A fusion protein of flagellin and ovalbumin as novel vaccine candidate for allergies: suppressing TH2 responses and preventing murine intestinal allergy

    S. Schuelke, M. Burggraf, Z. Waibler, A. Wangorsch, S. Wolfheimer, S. Vieths, M. Toda, S. Scheurer

    IMMUNOLOGY　137　435-435　2012/09

    ISSN： 0019-2805

  6. Interaction of vimentin and phosphorylated mitogen-activated protein kinases in Fc epsilon RI and CC chemokine receptor 1 engaged mast cells

    Chuan-Hui Kuo, Masako Toda, Satty Borman, Ricardo Richardson, Akihito Inoko, Masaki Inagaki, Klaus Schneider, Santa Ono

    JOURNAL OF IMMUNOLOGY　188　2012/05

    ISSN： 0022-1767

  7. Oral tolerance induction does not resolve allergic enteropathy due to residual IgE

    Masako Toda, Manja Burggraf, Haruyo Nakajima-Adachi, Satoshi Hachimura, Maren Krause, Hiroshi Kiyono, Groene Hermann-Josef, Stefan Vieths

    JOURNAL OF IMMUNOLOGY　188　2012/05

    ISSN： 0022-1767

  8. Protein unfolding strongly modulates allergenicity and immunogenicity of Pru p 3, the major peach allergen

    M. Toda, G. Reese, G. Gardemaier, Schulten, V, Lauer, I, M. Egger, P. Briza, S. Randow, S. Wolfheimer, Kigongo, V, M. Miguel Moncin, K. Foetisch, B. Bohle, S. Vieths, S. Scheurer

    ALLERGY　66　92-92　2011/06

    ISSN： 0105-4538

    eISSN： 1398-9995

  9. Macular and retinal dysfunction of unknown origin in adults with normal fundi: Evidence for an autoimmune pathophysiology (vol 84, pg 90, 2008)

    Irmela Mantel, Kanchan V. Ramchand, Graham E. Holder, Masaharu Ohbayashi, Kei Morohoshi, Nishal Patel, Masako Toda, Fred W. Fitzke, Alan C. Bird, Santa Jeremy Ono

    EXPERIMENTAL AND MOLECULAR PATHOLOGY　87　(2)　166-166　2009/10

    DOI： 10.1016/j.yexmp.2009.05.003 　

    ISSN： 0014-4800

  10. Differential gene expression with co-engagement of Fc epsilon RI and CC chemokine receptor 1

    Cho Cho Aye, Kei Morohoshi, Ken Fukuda, Nimita Fifadara, Masako Toda, Santa Jeremy Ono

    FASEB JOURNAL　22　2008/04

    ISSN： 0892-6638

  11. Glycation by the maillard reaction enhances the uptake of ovalbumin by murine dendritic cells

    A. Ilchmann, S. Burgdorf, Z. Waibler, C. Kurts, S. Scheurer, U. Kalinke, S. Vieths, M. Toda

    ALLERGY　63　411-411　2008

    ISSN： 0105-4538

  12. Glycation of ovalbumin by the Maillard reaction enhances allergen-specific activation of murine T cells

    A. Ilchmann, Z. Waibler, G. Reese, S. Scheurer, U. Kalinke, S. Vieths, M. Toda

    ALLERGY　62　4-4　2007/06

    ISSN： 0105-4538

  13. Rapidly progressive retinopathy with a normal fundus: Immune pathology

    KV Ramchand, Mantel, I, M Ohbayashi, N Patel, M Toda, KY Chau, AC Bird, SJ Ono

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　45　U986-U986　2004/04

    ISSN： 0146-0404

  14. Eotaxin-1 drives terminal differentiation of conjunctival mast cells

    T Nakamura, M Toda, D Miyazaki, ME Rothenberg, SJ Ono

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　44　U314-U314　2003/05

    ISSN： 0146-0404

  15. A novel role for eotaxin-1 in acute phase inflammation and mast cell degranulation

    T Nakamura, D Miyazaki, M Toda, KW Cheung-Chau, ME Rothenberg, SJ Ono

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　109　(1)　S70-S70　2002/01

    ISSN： 0091-6749

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Books and Other Publications 7

  1. Atopic Dermatitis: Inside Out or Outside In

    Michiko Shimoda, Khiem A. Tran, Masako Toda

    Elsevier　2022

    ISBN: 9780323847445

  2. グリケーションの制御とメイラード反応の利用（監修：有原圭三）

    戸田雅子

    シーエムシー出版　2020/12

    ISBN: 9784781315645

  3. アレルギー性腸炎マウスモデルにおいてCCR8は好酸球遊走を誘導し、好中球遊走を抑制する

    戸田雅子

    臨床免疫・アレルギー科　2020/04

  4. 症例を通して学ぶ年代別食物アレルギーのすべて」改訂2版（監修：海老澤 元宏）

    戸田 雅子, 穐山浩

    南山堂　2018/08

  5. 「Dietary AGEs and Their Role in Health and Disease: Edited by Jamie Uribarri」

    Masako Toda

    Taylor & Francis Group (USA)　2017

  6. 「メイラード反応の機構・制御・利用」（監修：宮澤陽夫）

    戸田 雅子

    シーエムシー出版　2016/04

  7. 「Handbook of eggs in promotion of health: Edited by Watson RR and De Meester F」

    Watanabe H, Takushi S, Nakajima-Adachi H, Hachimura S, Vieths S, Toda M

    2015

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Presentations 38

  1. The analysis of intestinal metabolome in a resolution model of food allergy

    Masako Toda, Elisa Zubeldia-Varela, Frank Blanco-Pérez, Thomas Barker-Tejeda, Konosuke Kameda, David Rojo, Alma Villaseñor, Coral Barbas, Stefan Vieths, Domingo Barber, Marina Pérez-Gordo

    第79回日本栄養·食糧学会大会　2025/05/25

  2. 高脂肪食飼育若齢および中高齢マウスにおけるビサクロン高含有ウコンエキスの抗炎症作用の解析

    上野 貴大、何 超琪、服部 浩之、松田 幹、宮澤 大樹、戸田 雅子

    日本農芸化学会2025年度大会　2025/03/06

  3. カンナビジオールとカンナビゲロールの免疫機能性の解析

    牛木 晴子, 黒石 智誠, 戸田 雅子

    日本農芸化学会2025年度大会　2025/03/05

  4. 酸化コレステロールおよびメバロン酸経路が樹状細胞の成熟に与える影響の解析

    山内 晴貴, 柳沢 林太郎, 浅井 明, 戸田 雅子

    日本農芸化学会2025年度大会　2025/03/05

  5. Immunomodulatory function of dietary fiber: impact on gut microbiome and metabolome and potential in allergy treatment Invited

    2024/12/02

  6. 高温高圧処理マグロに反応する小児における主要アレルゲンの解析

    久留宮岬, 堀野智史, 西尾俊亮, 松田幹, 三浦克志, 戸田雅子

    第73回日本アレルギー学会学術大会 2024年10月18日　2024/10/18

  7. Yeast-derived alpha-Mannan enhances IL-10 production in dendritic cells via multiple metabolic pathways (English sesstion/優秀発表賞)

    Masako Toda, Ting-Yu Cheng, Chizumi Abe, Taiki Miyazawa

    第73回日本アレルギー学会学術大会　2024/10/18

  8. Analysis of anti-allergy effect of yeast-derived α-mannan in a murine model of hen’s egg allergy

    郭 穎楠、何 超琪、鄭 廷宇、松田 幹、戸田 雅子

    第18回東北糖鎖研究会 2024年10月5日　2024/10/05

  9. α-Mannan enhances IL-10 production in dendritic cells via Dectin-2-mediated mTOR pathway activation（みちのく糖鎖ポスター賞）

    武藤優奈, 鄭廷宇, Stefan Schuelke, 服部浩之, 菅野恵美, 岩倉洋一郎, Stephan Scheurer, 戸田雅子

    第18回東北糖鎖研究会　2024/10/05

  10. クロレラ熱水抽出物（C.G.F）が結合するトル様受容体の同定

    クロレラ・機能性植物研究会　2024/09/28

  11. Yeast-derived alpha-Mannan enhances IL-10 production in dendritic cells via multiple metabolic pathways (Selected oral presentation)

    EAACI Congress　2024/06/03

  12. ： Invited

    2023/09/10

  13. SY-09 免疫制御の新展開: 食品・微生物成分による免疫制御のメカニズム Invited

    第77回 日本栄養食糧学会年次大会　2023/05/12

  14. Immunological Function of Chlorella Invited

    22nd IUNS-ICN　2022/12/11

  15. アレルゲン分子の改変： アレルゲン特異的免疫療法への応用を目指して Invited

    日本食品免疫学会第18回学術大会　2022/11/19

  16. 食物アレルギーのはなし Invited

    第28回室蘭工業大学蘭岳セミナー　2022/10/14

  17. Advanced Glycation End Products (AGEs) and Allergy Invited

    European Academy of Allergy & Clinical Immunology - ISMA-RHINA Digital 2022　2022/10/06

  18. 免疫機能および腸管機能の改善に寄与する 機能性食品成分と健康寿命の延伸について Invited

    ニューテクノロジー研究会　2021/12/07

  19. Immunological Function of Phytonutrients Invited

    Masako Toda

    2021/10/08

  20. 食物繊維の免疫機能性について Invited

    日本CG研究会セミナー　2021/09/11

  21. The role of mast cells in the development of experimental allergic enteritis

    2021/03/21

  22. 食のもつ抗アレルギーのポテンシャル Invited

    日本農芸化学会 2021年度仙台大会・化学と生物シンポジウム　2021/03/21

  23. Immunological function of dietary fiber Invited

    International symposium on lipids and food ingredients for health promotion　2021/03/12

  24. 免疫機能性素材としての農水産物由来多糖類 Invited

    産学官連携推進グリーンイノベーション研究会　2021/02/05

  25. アレルゲンの糖鎖修飾や糖化修飾がアレルギー発症に与える影響について Invited

    第43回日本分子生物学会年会フォーラム「糖質によるタンパク質の翻訳後修飾と疾患」　2020/12/02

  26. コレステロールと免疫 Invited

    戸田雅子

    植物油栄養懇話会　2020/09/15

  27. 食物アレルゲンのグリケ－ションが 食物アレルギ－の発症に及す影響について Invited

    第93回日本生化学会大会シンポジウム「グリケーションが心身健康発達に与える影響とその分子メカニズム」　2020/09/14

  28. Food Processing and Food Allergies Invited

    2020/02/20

  29. 疾患防御と食品機能成分の活用の可能性②：機能性糖類・食物繊維について Invited

    宮城県食品産業協議会・食品科学基礎講座2019　2019/11/23

  30. 食品産業のためのアレルギ－の基礎知識 Invited

    宮城県食品産業協議会・食品科学基礎講座2019　2019/11/23

  31. メイラード反応と食物アレルギ－ Invited

    第29回日本メイラード学会年会　2019/10/26

  32. アレルゲン分子の改変：より安全で効果的なアレルゲン特異的免疫療法を目指して Invited

    愛知県アレルギ－を語る会　2019/07/16

  33. 油脂・コレステロ－ルが免疫系に与える影響について Invited

    油脂・コレステロ－ル研究会　2019/07/15

  34. Mechanism for the intrinsic allergenicity of Ara h 1, a major peanut allergen, exerting activation of human primary macrophages

    Toda M, Krause M, Crauwels P, Globisch M, Henle T, van Zandbergen G, Vieths S, Scheurer S

    2019/03/27

  35. EUにおける研究動向からの考察する機能性食品の潮流 Invited

    JBA機能性食品研究会・第4回講演会　2019/02/25

  36. クロレラ由来成分の免疫機能性について Invited

    健康博覧会　2019/01/23

  37. Immunomodulatory Function of Rice Components International-presentation Invited

    Toda Masako

    The The Third International Symposium on Rice Science in Global Health　2018/11/30

  38. クロレラ・植物由来成分の 免疫機能性について Invited

    戸田 雅子

    クロレラ・機能性植物研究会　2018/10/29

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Research Projects 6

  1. ペクチンによる新規の抗アレルギー作用機構の解明

    System: 日独二国間交流事業

    2024/04 - 2026/03

  2. 酵母由来αマンナンの抗アレルギー作用の解析

    Offer Organization: 飯島藤十郎記念食品科学振興財団2023年度助成

    2024/04 - 2025/03

  3. 食品・微生物成分による抗炎症型ミエロイド系細胞の分化と機能発現メカニズムの解析

    戸田 雅子, 高橋 恭子, 宮澤 大樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04 - 2025/03

  4. 抗炎症作用を持つ食品成分が形成する自然免疫記憶についての解析

    戸田 雅子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2020/07 - 2023/03

    More details Close

    本研究は、食品成分を用いて「抗炎症作用を増強する自然免疫記憶」を樹状細胞に形成すること、さらにその形成メカニズムの解明により、食品の免疫調節性機能を増進することを目的とする。これまでに樹状細胞において、食品成分により抗炎症性サイトカインIL-10産生を増強する自然免疫を誘導できることを見いだしている。今年度は、自然免疫によるIL-10産生の増強メカニズムを解析した。自然免疫を誘導したマウス骨髄由来樹状細胞（Bone marrow derived murine dendritic cells）におけるIL-10遺伝子発現のエピジェネティクス制御について調べた。BMDCのIL-10産生はヒストン脱アセチル化酵素（HDAC）阻害剤 トリコスタチンAにより抑制される一方、IL-6産生は増強された。また、メチル基転移酵素非選択的阻害剤 Sinefunginやリシン脱メチル化酵素（KDM）阻害薬 トラニルシプロミンヘミ硫酸塩ではそのようなIL-10産生抑制は観察されなかった。さらに解析を進めた結果、ヒストン脱アセチル化酵素HDAC6 の選択的阻害によりBMDCにおけるIL-10産生は抑制された。以上より、樹状細胞における抗炎症性記憶形成には、HDAC6による脱アセチル化が関与することが示唆された。

  5. Analysis of immunological properties of mannose-based molecules and its action mechanisms.

    Toda Masako

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2019/04 - 2022/03

    More details Close

    The aim of this study was to identify immunological function of mannose-based molecules and its action mechanisms. Among α-mannooligosaccharides, long chain molecules such as α-Man-(1→6)-Man4 inhibited lipopolysaccharide (LPS)-stimulated activation of bone marrow derived murine dendritic cells (BMDC). In contrast, among β-mannooligosaccharides, β-Man-(1→4)-Man activated BMDC via engagement of toll-like receptor 4. We also found that α-mannan from yeast promotes anti-inflammatory responses (e.g., IL-10 production) in BMDC when it was stimulated with LPS. Metabolome analysis showed that α-mannan altered levels of many metabolites in BMDC, e.g., an increased level of lactic acid, the final product of glycolysis, and a reduced level of succinic acid, an inflammatory metabolite of TCA cycle. The results suggest that α-mannan induces reprogram of cellular metabolism and thereby promotes anti-inflammatory response in BMDC.

  6. 脂質過酸化反応が落花生アレルゲンのアレルゲン性に与える影響についての解析

    戸田 雅子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 研究活動スタート支援

    Institution: 東北大学

    2018/08 - 2020/03

    More details Close

    本研究では、脂質過酸化反応が落花生主要アレルゲンAra h 1のアレルゲン性に与える影響について解析した。まずは未処理の落花生、落花生オイルを加えて煎ることで脂質過酸化反応を誘導した落花生、オイルなしの加熱処理を行った落花生のタンパク質抽出物を調製した。さらに未処理の落花生からAra h 1を単離して、脂質過酸化反応の代表的な反応産物である2-ヘパノ－ル（2HP）や4-ヒドロキシノネナール（4HN）で修飾した。これらのサンプルのIgE反応性をwestern blottingやELISAにより解析した。落花生アレルギ－患者由来血清を用いたwestern blottingにおいては、加熱処理や2HPや4HNでの修飾によってAra h 1の二量体や三量体の形成が観察された。ELISA解析においては、サンプル間でIgE反応性の顕著な差は認められなかった。さらにサンプルの自然免疫活性化能をヒト単球由来樹状細胞を用いて解析した。未処理の落花生由来タンパク質抽出物やAra h 1による刺激により、樹状細胞における細胞表面マ－カ－（CD40やCD86、HLA-DR分子）の発現増強やサイトカイン（IL-6やTNF-α）の産生が誘導された。加熱処理をした落花生由来タンパク質抽出物や2HPや4HNにより修飾されたAra h 1も同様に樹状細胞を活性化した。一方で、糖鎖を持たないAra h 1組替え体は樹状細胞の活性化を誘導しなかった。以上から（1）落花生主要アレルゲンAra h 1の糖鎖が自然免疫活性化能に関わること、（2）脂質過酸化反応はAra h 1の自然免疫活性化能やIgE反応性、ひいてはアレルゲン性に影響を及さないことが示唆された。本研究で得られた知見は落花生アレルゲンの分子特性を明らかにするものであり、落花生アレルギ－発症のメカニズム解明に貢献する。

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Teaching Experience 6

  1. Food & Agricultural Immunology Joint Lecture (分担)

  2. Advanced Lecture on Food Science（分担）

  3. 食品化学

  4. 食品化学特論　東北大学

  5. 化学概論（東北大学）

  6. 免疫学　ダルムシュタット工科大学

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Social Activities 2

  1. 食品成分がもつ免疫制御のポテンシャル：第４回 －中小食品事業者に役に立つ食品科学

    2025/01/17 -

  2. 研究室紹介＠山形西高校オンライン大学訪問

    2024/12/26 -

Other 1

  1. ダルムシュタット工科大学生物学部 生物学・免疫学 教授資格取得