Details of the Researcher

PHOTO

Yasuhiro Miki
Section
Graduate School of Medicine
Job title
Associate Professor
Degree

  • 博士（医学）（東北大学）

Research History 7

  • 2022/04 - 2023/03
    Tohoku Bunka Gakuen University　Department of Nursing, Faculty of Medical Science & Welfare

  • 2012/11 - 2022/03
    東北大学災害科学国際研究所

  • 2010/04 - 2012/10
    東北大学大学院歯学研究科

  • 2007/04 - 2010/03
    東北大学大学院医学系研究科

  • 2006/04 - 2007/03
    日本学術振興会特別研究員DC

  • 1998/04 - 2003/03
    ボゾリサーチセンター

  • 2023/04 -
    東北大学大学院医学系研究科

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Education 2

  • Tohoku University　Graduate School, Division of Medicine

    - 2007/03/31

  • Rakuno Gakuen University　Faculty of Veterinary Medicine

    - 1998/03/31

Committee Memberships 4

  • 日本組織細胞化学会　評議員

    2020/01 - Present

  • 日本内分泌学会　評議員

    2012/04/01 - Present

  • 日本ステロイドホルモン学会　評議員

    2007/11 - Present

  • 日本内分泌学会　代議員

    2010/04 - 2012/03/31

Professional Memberships 6

  • The Endocrine Society(2011/08-)

  • 日本内分泌学会(2009/03- 評議員)

  • 日本乳癌学会(2007/01-)

  • 日本ステロイドホルモン学会(2007/11- 評議員)

  • 日本癌学会(2005/04-)

  • 日本内分泌学会(2001/04-)

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Research Interests 8

  • 災害科学

  • 病理学

  • 内分泌学

  • 腫瘍学

  • 癌微小環境

  • 肺癌

  • 子宮内膜癌

  • 乳癌

Research Areas 1

  • Life sciences / Tumor biology /

Awards 7

  1. Breast Cancer Reviewer 賞

    2020　日本乳癌学会

  2. 第4回研究奨励賞

    2019/11　日本ステロイドホルモン学会

  3. Journal of Steroid Biochemistry and Molecular Biology 2013 Excellence in Reviewing

    2014/11　Elsevier B.V.

  4. 第17回日本乳癌学会研究奨励賞

    2011/09/02　日本乳癌学会

  5. 伊豆レスピロロジーフォーラムYoung Investigators Award

    2009/08　伊豆レスピロロジーフォーラム

  6. 平成19年度東北大学医学部奨学賞銀賞ならびに坂田賞

    2008/01/18　東北大学医学部

  7. 平成19年度宮城県医師会医学奨励賞

    2008/01/05　宮城県医師会

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Papers 368

  1. Tetraspanins CD63 and CD81 as potential prognostic biomarkers in breast cancer.

    Erina Iwabuchi, Yasuhiro Miki, Junyao Xu, Ayako Kanai, Takanori Ishida, Takashi Suzuki

    Breast cancer (Tokyo, Japan)　32　(2)　393-403　2025/03

    DOI： 10.1007/s12282-025-01666-x 　

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    Exosome markers, CD63 and CD81, belong to the tetraspanin family and are expressed in solid tumors. It has been reported that these tetraspanin family members are prognostic factors in some cancers. However, the expression of CD63 and CD81 in pathological breast cancer specimens has not been reported. It has been reported that CD63 promotes the proliferation of breast cancer cells in vitro through yes-associated protein (YAP). Therefore, in this study, the expression of tetraspanin family members, particularly CD63, CD81, and YAP were investigated in breast cancer tissue, by immunohistochemistry, to clarify the relationship between clinicopathological factors and prognosis. The number of CD63 and YAP double-positive breast cancer cells was significantly higher in patients with pathological T factor (pT) status (p = 0.030) and tended to be higher in patients with pathological N factor (pN) status (p = 0.054). Furthermore, the number of CD81 and YAP double-positive breast cancer cells was significantly higher in patients with histological grade (p = 0.015), pT status (p = 0.001), and Ki67 expression (p = 0.049), and tended to be higher in patients with pN status (p = 0.062) and TNM stage (p = 0.052). In addition, CD63 and YAP double-positive breast cancers and CD81 and YAP double-positive breast cancers were associated with shorter disease-free and breast cancer-specific survival, respectively. In conclusion, CD63 and YAP, and CD81 and YAP may serve as potential prognostic biomarkers in patients with breast cancer.

  2. Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

    Ai Sato, Kiyoshi Takagi, Momoka Yoshida, Mio Yamaguchi-Tanaka, Mikoto Sagehashi, Yasuhiro Miki, Minoru Miyashita, Takashi Suzuki

    Cancers　16　(24)　4285-4285　2024/12/23

    Publisher: MDPI AG

    DOI： 10.3390/cancers16244285 　

    eISSN： 2072-6694

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    Background: Chemoresistance is an important issue to be solved in breast cancer. It is well known that the content and morphology of collagens in tumor tissues are drastically altered following chemotherapy, and discoidin domain receptor 2 (DDR2) is a unique type of receptor tyrosine kinase (RTK). This RTK is activated by collagens, playing important roles in human malignancies. However, the contribution to the chemoresistance of DDR2 in terms of the association with collagens remains largely unclear in breast cancer. Methods: We immunolocalized DDR2 and collagen type I in 224 breast cancer tissues and subsequently conducted in vitro studies to confirm the role of DDR2 in breast cancer chemoresistance using chemosensitive and chemoresistant cell lines. Results: DDR2 immunoreactivity was positively correlated with aggressive behaviors of breast cancer and was significantly associated with an increased risk of recurrence, especially in those who received chemotherapy. Moreover, in vitro experiments demonstrated that DDR2 promoted the proliferative activity of breast cancer cells, and cell viability after epirubicin treatment was significantly maintained by DDR2 in a collagen I-dependent manner. Conclusions: These data suggested that DDR2 could be a poor prognostic factor associated with cell proliferation and chemotherapy resistance in human breast cancer.

  3. Analyzing Trends in the Medical Community During the Spanish Flu Pandemic in Japan with the Comprehensive Knowledge of Humanities and Sciences: A Case Study of the Medical Journal, The Japan Medical World

    Atsushi Kawauchi, Natsuko Chubachi, Yasuhiro Miki, Kiyoshi Ito, Eiichi N. Kodama

    Journal of Disaster Research　19　(6)　921-934　2024/12/01

    Publisher: Fuji Technology Press Ltd.

    DOI： 10.20965/jdr.2024.p0921 　

    ISSN： 1881-2473

    eISSN： 1883-8030

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    This study aimed to examine the trends in the medical community in Japan during the Spanish flu pandemic by analyzing The Japan Medical World (JMW), a medical journal of modern Japan. The analysis included 3,856 articles published in issues Nos.228–279, from January 1918 to March 1919. Three key issues emerged from this analysis. First, the tone of the JMW’s discourse may have been influenced by the prevailing medical situation or may have placed emphasis on specific aspects of the situation. Second, the JMW and the medical community in Japan initially neglected the influenza pandemic until the autumn of 1918, when the Spanish flu became widespread in Japan. Third, complex factors, such as academic factionalism and conflicts within the pharmaceutical industries, influenced the Japanese medical community, particularly the bacteriological scientific community during the Spanish flu pandemic. Therefore, “a comprehensive approach” to understanding the period, including not only medical knowledge but also critical reading skills of humanities and social sciences, is indispensable for analyzing the trends in the Japanese medical community during the Spanish flu pandemic. Attention should be paid to these factors when examining the trends in the medical community in Japan at that time.

  4. Receptor for Hyaluronan Mediated Motility (RHAMM)/Hyaluronan Axis in Breast Cancer Chemoresistance

    Shiori Fujisawa, Kiyoshi Takagi, Mio Yamaguchi-Tanaka, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Hiroshi Tada, Takanori Ishida, Takashi Suzuki

    Cancers　16　(21)　3600-3600　2024/10/25

    Publisher: MDPI AG

    DOI： 10.3390/cancers16213600 　

    eISSN： 2072-6694

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    Background/Objectives: Receptor for hyaluronan-mediated motility (RHAMM) is a hyaluronan (HA) receptor, which exerts diverse biological functions in not only physiological but also pathological conditions in human malignancies, including breast cancer. Although chemoresistance is a significant clinical challenge in breast cancer, a possible contribution of RHAMM and hyaluronan to breast cancer chemoresistance has remained unclear. Methods: We immunolocalized RHAMM and HA in breast carcinoma tissues. Also, we utilized epirubicin-sensitive (parental) and rpirubicin-resistant (EPIR) breast cancer cell lines to explore the role of RHAMMM in breast cancer progression. Results: We found out that RHAMM and HA were cooperatively correlated with breast cancer aggressiveness and recurrence after chemotherapy. In vitro studies demonstrated that RHAMM was overexpressed in EPIR cells compared to parental cells. In addition, the knockdown of RHAMM significantly suppressed proliferation and migration of both parental and EPIR cells. On the other hand, the expression level of cancer stem cell marker CD44, which was overexpressed in M-EPIR (epirubicin-resistant MCF-7 subline) compared to MCF-7, was significantly suppressed by knockdown of RHAMM. In addition, the knockdown of RHAMM significantly altered the expression of N-cadherin and E-cadherin, leading to an epithelial phenotype. Conclusions: Aberrant RHAMM signaling were considered to cause chemoresistance related to cancer stemness and epithelial to mesenchymal transition, and increased cell proliferation and migration of both chemo-sensitive and chemo-resistant breast cancer cells.

  5. Prognostic significance and response to immune checkpoint inhibitors of RIPK3, MLKL and necroptosis in non-small cell lung cancer. International-journal

    Nattaya Duangthim, Thanpisit Lomphithak, Ryoko Saito-Koyama, Yasuhiro Miki, Chihiro Inoue, Ikuro Sato, Eisaku Miyauchi, Jiro Abe, Hironobu Sasano, Siriporn Jitkaew

    Scientific reports　14　(1)　21625-21625　2024/09/16

    DOI： 10.1038/s41598-024-72545-2 　

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    Lung cancer remains the leading cause of cancer death. Treatment with immune checkpoint inhibitor (ICI) alone or combination with chemotherapy served as first-line therapy in non-small cell lung cancer (NSCLC). However, only 20-50% of NSCLC patients respond to ICI. Necroptosis, an inflammatory form of cell death plays an important role in the regulation of tumor immune microenvironment which may affect prognosis and ICI response but its clinical significance in NSCLC patients has remained largely unknown. Therefore, we aimed to analyze the correlation between key necroptotic proteins and necroptosis and clinical outcomes, the status of tumor-infiltrating immune cells, and response to ICI in NSCLC patients. The expression of receptor-interacting protein kinase 3 (RIPK3), mixed lineage kinase domain-like protein (MLKL) and phosphorylated MLKL (pMLKL) were immunolocalized in 125 surgically resected NSCLC patients and 23 NSCLC patients administered with ICI therapy. CD8 + and FOXp3 + T cells and CD163 + M2 macrophages were also immunolocalized. High RIPK3 status was positively correlated with survival of the patients and RIPK3 turned out an independent favorable prognostic factor of the patients. RIPK3 was negatively correlated with CD8 + T cells, while MLKL positively correlated with CD163 + M2 macrophages, suggesting the possible involvement of RIPK3 and MLKL in formulating immunosuppressive microenvironment. In addition, high RIPK3 status tended to be associated with clinical resistance to ICI therapy (P-value = 0.057). Furthermore, NSCLC cells-expressing RIPK3 suppressed T cells response to ICI therapy in vitro. Therefore, RIPK3 and MLKL could induce an immunosuppressive microenvironment, resulting in low response to ICI therapy in NSCLC.

  6. Zinc transporter ZnT5 is associated with epithelial mesenchymal transition via SMAD1 in breast cancer. International-journal

    Erina Iwabuchi, Yasuhiro Miki, Junyao Xu, Ayako Kanai, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    International journal of experimental pathology　2024/08/13

    DOI： 10.1111/iep.12515 　

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    Zinc levels in breast cancer tissues have been reported to be higher than those in normal tissues. In addition, the expression levels of zinc transporters, including ZnT5 and ZnT6, are reportedly higher in breast cancer than in normal breast tissues. ZnT5 and ZnT6 also contribute to heterodimer formation and are involved in several biological functions. However, the functions of ZnT5 and ZnT6 heterodimers in breast cancer remain unknown. Therefore, we first investigated the immunolocalization of ZnT5 and ZnT6 in pathological breast cancer specimens and in MCF-7 and T-47D breast cancer cells. Next, we used small interfering RNA to assess cell viability and migration in ZnT5 knockdown MCF-7 and T-47D cells. Immunohistochemical analysis showed that the number of ZnT5-positive breast cancer cells was inversely correlated with the pathologic N factor status. ZnT5 knockdown had no effect on cell viability in the presence of 100 μM ZnCl2 in MCF-7 and T-47D cells. In a wound healing assay, 100 μM ZnCl2 treatment inhibited cell migration of MCF-7 and T-47D cells, whereas ZnT5 knockdown promoted cell migration, decreased E-cadherin expression and increased vimentin, slug and matrix metalloproteinase 9 expression. Antibody arrays showed that ZnT5 knockdown increased the expression of SMAD1, and that dorsomorphin treatment inhibited the promotion of migratory ability induced by ZnT5 knockdown. The results of this study revealed that both ZnT5 may be involved in less aggressive breast cancer subtypes, possibly through inhibition of cell migration.

  7. Histopathological analysis potential for unveiling hormone signaling in endocrine-related tumors

    Yasuhiro Miki, Erina Iwabuchi, Chihiro Inoue, Yuto Yamazaki, Takashi Suzuki

    Endocrine Oncology　2024/08

    Publisher: Bioscientifica

    DOI： 10.1530/eo-24-0033 　

    ISSN： 2634-4793

    eISSN： 2634-4793

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    Elucidating the mechanisms of action of steroid hormones will contribute to the development of therapeutic strategies for hormone-dependent tumors. Recent advances in genetic engineering have revealed the complex and diverse mechanisms of steroid hormone signaling; however, these techniques are limited to in vitro or animal experiments. It is believed that verifying hormone signals elucidated using human pathological tissue specimens will directly aid in treatment and diagnosis. However, pathological tissue specimens are generally formalin-fixed paraffin-embedded (FFPE), and protein/gene analyses of FFPE tissues are limited. Protein detection using immunohistochemistry with specific antibodies in FFPE tissues is a classical technique essential for diagnosis and treatment decisions in various types of cancer. In steroid hormone signaling, the expression and localization of receptors, hormone-related enzymes, and proteins encoded by response genes can be clarified using immunohistochemistry. Although protein-protein interactions such as receptor dimers and DNA-binding proteins are mainly detected in vitro, they can be examined in FFPE tissues using in situ proximity ligation assays and southwestern histochemistry, respectively. Using these detection methods, including immunohistochemistry, it is possible to analyze each hormone signaling pathway in hormone-related tumors histopathologically. Although FFPE tissues still suffers from gene and protein denaturation, its advantages include the ability to retrospectively study target factors/signals and obtain spatial information through microscopy. This review describes a visualization method for elucidating steroid hormone signaling in hormone-dependent tumors using FFPE tissues.

  8. Dipeptidyl peptidase 4-positive cancer-associated fibroblasts enhance lung adenocarcinoma growth. International-journal

    Chihiro Inoue, Yasuhiro Miki, Ryoko Saito-Koyama, Yoshinori Okada, Hironobu Sasano, Takashi Suzuki

    Pathology, research and practice　260　155418-155418　2024/06/20

    DOI： 10.1016/j.prp.2024.155418 　

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    Cancer-associated fibroblasts (CAFs) are a heterogeneous population of fibroblasts with various features in the cancer stroma and have been reported to influence cancer progression through cell-cell interactions in various types of malignancies, including lung adenocarcinoma (LUAD). Dipeptidyl peptidase 4 (DPP4) is a transmembrane protein with serine protease activity and is involved in the progression of tumors, metabolic diseases, and autoimmune diseases. In the present study, we focused on the role of DPP4-positive CAFs in LUAD. Immunohistochemistry revealed that 38 of 89 LUAD patients showed DPP4 expression in the fibrous stroma, and patients harboring DPP4-positive CAFs were more often male, had a higher Brinkman index, and had a higher Ki-67 labeling index of tumor cells than those with DPP4-negative CAFs. DPP4-positivity was associated with the expression of other CAF markers, α-SMA, periostin, and podoplanin, as well as a cellular senescence marker, p16. In the in vitro study, conditioned media collected from pulmonary fibroblast (OUS-11, HPF, and HPF-C)-induced overexpression of DPP4 significantly promoted the proliferation of LUAD cells (A549 and PC-9) and increased the expression levels of MCP-1, IL-8, IL-6, and GCSF in the media compared to those in controls. In addition, OUS-11 overexpression in DPP4 overexpression increased periostin expression. In conclusion, DPP4-positive CAFs could promote lung adenocarcinoma cell growth by producing soluble factors, and DPP4 inhibition may inhibit cancer progression.

  9. Clinicopathological significance of hyaluronan and hyaluronidase 2 (HYAL2) in breast cancer

    Shiori Fujisawa, Kiyoshi Takagi, Mio Yamaguchi-Tanaka, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Hiroshi Tada, Takanori Ishida, Takashi Suzuki

    Pathology - Research and Practice　155434-155434　2024/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.prp.2024.155434 　

    ISSN： 0344-0338

  10. Toll-like receptor (TLR) 4 is a potent prognostic factor in prostate cancer associated with proliferation and invasion. International-journal

    Iku Takahashi, Kiyoshi Takagi, Mio Yamaguchi-Tanaka, Ai Sato, Masahiko Sato, Yasuhiro Miki, Akihiro Ito, Takashi Suzuki

    Pathology, research and practice　260　155379-155379　2024/05/29

    DOI： 10.1016/j.prp.2024.155379 　

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    Prostate cancer is one of the most common malignancies in men, and there is a need to explore novel biomarkers or therapeutic targets. Toll-like receptor 4 (TLR4) is expressed not only in antigen-presenting cells but also types of human malignancies, contributing to disease progression, although its clinical significance or functional role in prostate cancer remains unclear. Therefore, we immunolocalized TLR4 in 117 prostate cancer tissues to address its clinicopathological significance. Additionally, we performed in vitro assays to examine the effects of TLR4 on proliferation and migration of prostate cancer cell lines (LNCaP, DU-145 and PC-3). TLR4 immunoreactivity was predominantly detected in the cytoplasm of prostate cancer cells, and it was positively associated with proliferation and invasion abilities, as well as Gleason score. Subsequent in vitro experiments revealed that the inhibition of TLR4 by Sparstolonin B (SsnB) significantly suppressed the proliferation and migration of LNCaP, DU-145 and PC-3 cells. Therefore, we concluded that TLR4 was a potent prognostic factor associated with proliferation and invasion, and it might serve as a therapeutic target in prostate cancer.

  11. Clinicopathological Significance and Prognostic Role of High Mobility Group Box 1 (HMGB1), Toll-Like Receptor (TLR) 2 and TLR4 in Breast Cancer

    Reina Taguchi, Mio Yamaguchi-Tanaka, Kiyoshi Takagi, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Takashi Suzuki

    ACTA HISTOCHEMICA ET CYTOCHEMICA　57　(2)　75-83　2024/04/25

    Publisher: Japan Society of Histochemistry & Cytochemistry

    DOI： 10.1267/ahc.24-00006 　

    ISSN： 0044-5991

    eISSN： 1347-5800

  12. 肺腺癌での癌関連線維芽細胞におけるintegrin発現と臨床病理学的因子の相関についての検討

    柴田 沙織, 井上 千裕, 三木 康宏, 野津田 泰嗣, 新井川 弘道, 岡田 克典, 鈴木 貴

    日本呼吸器外科学会雑誌　38　(3)　MO18-4　2024/04

    Publisher: (一社)日本呼吸器外科学会

    ISSN： 0919-0945

    eISSN： 1881-4158

  13. Progesterone receptor-Grb2 interaction is associated with better outcomes in breast cancer. International-journal

    Nattamolphan Wittayavimol, Erina Iwabuchi, Prangwan Pateetin, Yasuhiro Miki, Yoshiaki Onodera, Hironobu Sasano, Viroj Boonyaratanakornkit

    The Journal of steroid biochemistry and molecular biology　237　106441-106441　2024/03

    DOI： 10.1016/j.jsbmb.2023.106441 　

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    In addition to mediating nuclear transcription, PR mediates extranuclear functions mainly through the PR polyproline domain (PPD) interaction with the SH3 domain of cytoplasmic signaling molecules. PR-PPD-SH3 interaction inhibits EGF-mediated signaling and decreases lung cancer cell proliferation. Grb2 is an essential adaptor molecule with an SH2 domain flanked by two SH3 domains. In this study, we examined whether PR, through interaction between PR-PPD and Grb2-SH3, can interact with Grb2 in cells and breast cancer tissues. Our previous study shows that interaction between PR-PPD and Grb2 could interfere with cytoplasmic signaling and lead to inhibition of EGF-mediated signaling. GST-pulldown analysis shows that PR-PPD specifically interacts with the SH3 domains of Grb2. Immunofluorescence staining shows colocalization of PR and Grb2 in both the nucleus and cytoplasm in BT-474 breast cancer cells. Using Bimolecular Fluorescence Complementation (BiFC) analysis, we show that PR and Grb2 interact in breast cancer cells through the Grb2-SH3 domain. Proximity Ligation Assay (PLA) analysis of 43 breast cancer specimens shows that PR-Grb2 interaction is associated with low histological stage and negatively correlates with lymph node invasion and metastasis in breast cancer. These results, together with our previous findings, suggest that PR-PPD interaction with Grb2 plays an essential role in PR-mediated growth factor signaling inhibition and could contribute significantly to better prognosis in PR- and Grb2-positive breast cancer. Our finding provides a basis for additional studies to explore a novel therapeutic strategy for cancer treatment.

  14. Pronecroptotic Therapy Using Ceramide Nanoliposomes Is Effective for Triple-Negative Breast Cancer Cells

    Yuki Ohya, Yuri Ogiso, Masaya Matsuda, Harumi Sakae, Kentaro Nishida, Yasuhiro Miki, Todd E. Fox, Mark Kester, Wataru Sakamoto, Takeshi Nabe, Kazuyuki Kitatani

    Cells　13　(5)　405-405　2024/02/26

    Publisher: MDPI AG

    DOI： 10.3390/cells13050405 　

    eISSN： 2073-4409

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    Regulated necrosis, termed necroptosis, represents a potential therapeutic target for refractory cancer. Ceramide nanoliposomes (CNLs), considered potential chemotherapeutic agents, induce necroptosis by targeting the activating protein mixed lineage kinase domain-like protein (MLKL). In the present study, we examined the potential of pronecroptotic therapy using CNLs for refractory triple-negative breast cancer (TNBC), for which there is a lack of definite and effective therapeutic targets among the various immunohistological subtypes of breast cancer. MLKL mRNA expression in tumor tissues was significantly higher in TNBC patients than in those with non-TNBC subtypes. Similarly, among the 50 breast cancer cell lines examined, MLKL expression was higher in TNBC-classified cell lines. TNBC cell lines were more susceptible to the therapeutic effects of CNLs than the non-TNBC subtypes of breast cancer cell lines. In TNBC-classified MDA-MB-231 cells, the knockdown of MLKL suppressed cell death induced by CNLs or the active substance short-chain C6-ceramide. Accordingly, TNBC cells were prone to CNL-evoked necroptotic cell death. These results will contribute to the development of CNL-based pronecroptotic therapy for TNBC.

  15. 肺腺癌の癌関連線維芽細胞におけるインテグリンファミリー発現と生命予後との関連性(Immunohistochemical study on integrin family in cancer-associated fibroblasts of lung adenocarcinoma)

    井上 千裕, 柴田 沙織, 三木 康宏, 野津田 泰嗣, 新井川 弘道, 岡田 克典, 鈴木 貴

    日本病理学会会誌　113　(1)　369-369　2024/02

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  16. Intratumoral cortisol associated with aromatase in the endometrial cancer microenvironment. International-journal

    Yasuhiro Miki, Erina Iwabuchi, Kiyoshi Takagi, Yuto Yamazaki, Yusuke Shibuya, Hideki Tokunaga, Muneaki Shimada, Takashi Suzuki, Kiyoshi Ito

    Pathology, research and practice　251　154873-154873　2023/10/06

    DOI： 10.1016/j.prp.2023.154873 　

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    Glucocorticoids bind to glucocorticoid receptors (GR). In the peripheral tissues, active cortisol is produced from inactive cortisone by 11β-hydroxysteroid dehydrogenase (HSD)1. 11β-HSD2 is responsible for this reverse catalysis. Although GR and 11β-HSDs have been reported to be involved in the malignant behavior of various cancer types, the concentration of glucocorticoids in cancer tissues has not been investigated. In this study, we measured glucocorticoids in serum and cancer tissues using liquid chromatography-tandem mass spectrometry and clarified, for the first time, the intratumoral "intracrine" production of cortisol by 11β-HSD1/2 in endometrial cancer. Intratumoral cortisol levels were high in the high-malignancy type and the cancer proliferation marker Ki-67-high group, suggesting that cortisol greatly contributes to the malignant behavior of endometrial cancer. A low expression level of the metabolizing enzyme 11β-HSD2 is more important than a high expression level of the synthase 11β-HSD1 for intratumoral cortisol action. Intratumoral cortisol was positively related to the expression/activity of estrogen synthase aromatase, which involved GR expressed in fibroblastic stromal cells but not in cancer cells. Blockade of GR signaling by hormone therapy is expected to benefit patients with endometrial cancer.

  17. SGLT1 as an adverse prognostic factor in invasive ductal carcinoma of the breast. International-journal

    Satoko Tsunokake, Erina Iwabuchi, Yasuhiro Miki, Ayako Kanai, Yoshiaki Onodera, Hironobu Sasano, Takanori Ishida, Takashi Suzuki

    Breast cancer research and treatment　201　(3)　499-513　2023/10

    DOI： 10.1007/s10549-023-07024-9 　

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    PURPOSE: Sodium/glucose cotransporter (SGLT) 1 and 2 expression in carcinoma cells was recently examined, but their association with the clinicopathological factors of the patients and their biological effects on breast carcinoma cells have remained remain virtually unknown. Therefore, in this study, we explored the expression status of SGLT1 and SGLT2 in breast cancer patients and examined the effects of SGLT1 inhibitors on breast carcinoma cells in vitro. METHODS: SGLT1 and SGLT2 were immunolocalized and we first correlated the findings with clinicopathological factors of the patients. We then administered mizagliflozin and KGA-2727, SGLT1 specific inhibitors to MCF-7 and MDA-MB-468 breast carcinoma cell lines, and their growth-inhibitory effects were examined. Protein arrays were then used to further explore their effects on the growth factors. RESULTS: The SGLT1 high group had significantly worse clinical outcome including both overall survival and disease-free survival than low group. SGLT2 status was not significantly correlated with clinical outcome of the patients. Both mizagliflozin and KGA-2727 inhibited the growth of breast cancer cell lines. Of particular interest, mizagliflozin inhibited the proliferation of MCF-7 cells, even under very low glucose conditions. Mizagliflozin downregulated vascular endothelial growth factor receptor 2 phosphorylation. CONCLUSION: High SGLT1 expression turned out as an adverse clinical prognostic factor in breast cancer patient. This is the first study demonstrating that SGLT1 inhibitors suppressed breast carcinoma cell proliferation. These results indicated that SGLT1 inhibitors could be used as therapeutic agents for breast cancer patients with aggressive biological behaviors.

  18. 乳癌随伴マクロファージにおけるホスファチジルセリン受容体TIM4の役割(The role of the phosphatidylserine receptor TIM4 expressed on macrophages in breast cancers)

    田中 美桜, 高木 清司, 高橋 美佑, 佐藤 和, 三木 康宏, 宮下 穣, 石岡 千加史, 鈴木 貴, 石岡 千加史

    日本癌学会総会記事　82回　1938-1938　2023/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  19. 乳癌の化学療法耐性における14-3-3ζの役割(The role of 14-3-3ζ in breast cancer chemoresistance)

    高橋 郁, 高木 清司, 田中 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 鈴木 貴

    日本癌学会総会記事　82回　2121-2121　2023/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  20. 乳癌におけるコンドロイチン硫酸-Cの発現意義(Prognostic role of chondroitin sulfate-C in breast cancer)

    佐藤 結名子, 高木 清司, 田中 美桜, 藤沢 詩織, 佐藤 和, 三木 康宏, 宮下 穣, 鈴木 貴

    日本癌学会総会記事　82回　2122-2122　2023/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  21. 乳癌の治療抵抗性におけるHMGB1/TLR2,4の役割(HMGB1/TLR2,4 axis is associated with therapeutic resistance of human breast cancer)

    高木 清司, 田口 玲奈, 田中 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 鈴木 貴

    日本癌学会総会記事　82回　2124-2124　2023/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  22. 乳癌におけるRHAMMとヒアルロン酸の発現意義(The role of RHAMM and Hyaluronan in human breast carcinoma)

    藤沢 詩織, 高木 清司, 田中 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 多田 寛, 鈴木 貴, 石田 孝宣

    日本癌学会総会記事　82回　1939-1939　2023/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  23. Immunolocalization of Cytoplasmic ER in ER-negative Breast Carcinoma as a Potent Favorable Prognostic Predictor.

    Akiko Ebata, Takashi Suzuki, Narumi Shoji-Harada, Yohei Hamanaka, Minoru Miyashita, Erina Iwabuchi, Kiyoshi Takagi, Yasuhiro Miki, Hiroshi Tada, Takanori Ishida

    Acta histochemica et cytochemica　56　(4)　59-66　2023/08/30

    DOI： 10.1267/ahc.23-00016 　

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    It is known that estrogen receptor (ER) has extranuclear signaling functions in addition to classical genomic pathway, and estrogenic actions have been reported in ER-negative breast carcinoma cells. However, significance of cytoplasmic-ER immunoreactivity has not been reported in ER-negative breast carcinoma tissues. We immunolocalized cytoplasmic ER in 155 ER-negative breast carcinoma tissues and evaluated its clinicopathological significance including the prognosis. As a comparative cohort set, we also used 142 ER-positive breast carcinomas. Cytoplasmic-ER immunoreactivity was detected in the carcinoma cells, but not in the non-neoplastic mammary epithelium. Cytoplasmic-ER immunoreactivity was positive in the 35 out of 155 (23%) ER-negative breast carcinoma cases, whereas it was detected only in 2 out of 142 (1.4%) ER-positive cases. Cytoplasmic ER status was positively associated with cytoplasmic-PR status, but inversely associated with Ki67 labeling index or distant free-relapse survival rate. Moreover, cytoplasmic-ER status turned out to be an independent good prognostic factor for both distant relapse-free survival and breast cancer specific survival. These findings suggested that cytoplasmic ER plays important roles in the ER-negative breast carcinoma, and cytoplasmic ER is a potent good prognostic factor. Among the ER-negative breast cancer patients, clinical benefit of chemotherapy may be limited in the cytoplasmic-ER positive cases.

  24. New Perspectives on Sex Steroid Hormones Signaling in Cancer-Associated Fibroblasts of Non-Small Cell Lung Cancer International-journal Peer-reviewed

    Chihiro Inoue, Yasuhiro Miki, Takashi Suzuki

    Cancers　15　(14)　2023/07/14

    DOI： 10.3390/cancers15143620 　

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    The importance of sex hormones, especially estrogen, in the pathogenesis of non-small-cell lung cancer (NSCLC) has attracted attention due to its high incidence among young adults and nonsmokers, especially those who are female. Cancer-associated fibroblasts (CAFs) reside in the cancer stroma and influence cancer growth, invasion, metastasis, and acquisition of drug resistance through interactions with cancer cells and other microenvironmental components. Hormone-mediated cell-cell interactions are classic cell-cell interactions and well-known phenomena in breast cancer and prostate cancer CAFs. In cancers of other organs, including NSCLC, the effects of CAFs on hormone-receptor expression and hormone production in cancer tissues have been reported; however, there are few such studies. Many more studies have been performed on breast and prostate cancers. Recent advances in technology, particularly single-cell analysis techniques, have led to significant advances in the classification and function of CAFs. However, the importance of sex hormones in cell-cell interactions of CAFs in NSCLC remains unclear. This review summarizes reports on CAFs in NSCLC and sex hormones in cancer and immune cells surrounding CAFs. Furthermore, we discuss the prospects of sex-hormone research involving CAFs in NSCLC.

  25. 乳癌における膜型アンドロゲン受容体ZIP9の発現意義

    田中 美桜[山口], 橋場 克幸, 高木 清司, 佐藤 和, 江幡 明子, 三木 康宏, 宮下 穣, 鈴木 貴

    日本内分泌学会雑誌　99　(3)　718-718　2023/07

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  26. Hormone-Dependent Cancers: New Aspects on Biochemistry and Molecular Pathology. International-journal

    Yasuhiro Miki

    International journal of molecular sciences　24　(13)　2023/06/29

    DOI： 10.3390/ijms241310830 　

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    Hormones, especially steroids, are closely involved in the physiological functions and proliferation of various target tissues and have long been known to play a key role in the tumorigenesis or carcinogenesis of these target tissues [...].

  27. Induction of SGK1 via glucocorticoid-influenced clinical outcome of triple-negative breast cancer patients. International-journal

    Junjia Zhang, Yasuhiro Miki, Erina Iwabuchi, Junyao Xu, Ayako Kanai, Yasuaki Sagara, Yasuyo Ohi, Yoshiaki Rai, Rin Yamaguchi, Maki Tanaka, Takanori Ishida, Takashi Suzuki, Hironobu Sasano

    Breast cancer research and treatment　200　(3)　323-335　2023/06/07

    DOI： 10.1007/s10549-023-06990-4 　

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    PURPOSE: Triple-negative breast cancer (TNBC) is a highly heterogeneous and aggressive breast malignancy. Glucocorticoid (GC)-glucocorticoid receptor (GR) pathway plays a pivotal role in the cellular responses to various stresses including chemotherapy. Serum- and glucocorticoid-induced kinase-1 (SGK1) is known as an important downstream effector molecule in the GR signaling pathway, we attempted to explore its clinicopathological and functional significance in TNBC in which GR is expressed. METHODS: We first immunolocalized GR and SGK1 and correlated the results with clinicopathological variables and clinical outcome in 131 TNBC patients. We also evaluated the effects of SGK1 on the cell proliferation and migration in TNBC cell lines with administration of dexamethasone (DEX) to further clarify the significance of SGK1. RESULTS: The status of SGK1 in carcinoma cells was significantly associated with adverse clinical outcome in TNBC patients examined and was significantly associated with lymph node metastasis, pathological stage, and lymphatic invasion of the patients. In particular, SGK1 immunoreactivity was significantly associated with an increased risk of recurrence in GR-positive TNBC patients. Subsequent in vitro studies also demonstrated that DEX promoted TNBC cell migration and the silencing of gene expression did inhibit the cell proliferation and migration of TNBC cells under DEX treatment. CONCLUSIONS: To the best of our knowledge, this is the first study to explore an association between SGK1 and clinicopathological variables and clinical outcome of TNBC patients. SGK1 status was significantly positively correlated with adverse clinical outcome of TNBC patients and promoted carcinoma cell proliferation and migration of carcinoma cells.

  28. 乳癌におけるRHAMMの発現意義

    藤沢 詩織, 高木 清司, 田中 美桜[山口], 佐藤 和, 三木 康宏, 宮下 穣, 多田 寛, 石田 孝宣, 鈴木 貴

    日本乳癌学会総会プログラム抄録集　31回　440-440　2023/06

    Publisher: (一社)日本乳癌学会

  29. Interleukin (IL)-17A in triple-negative breast cancer: a potent prognostic factor associated with intratumoral neutrophil infiltration.

    Freeha Khalid, Kiyoshi Takagi, Ai Sato, Mio Yamaguchi, Fouzia Guestini, Yasuhiro Miki, Minoru Miyashita, Hisashi Hirakawa, Yasuyo Ohi, Yoshiaki Rai, Yasuaki Sagara, Hironobu Sasano, Takashi Suzuki

    Breast cancer (Tokyo, Japan)　30　(5)　748-757　2023/05/13

    DOI： 10.1007/s12282-023-01467-0 　

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    BACKGROUND: Triple-negative breast cancer (TNBC) is characterized as highly immunogenic and lacks specific targeted therapies. Interleukin 17A (IL-17A) is a controversial cytokine and is known to have anti-tumor and pro-tumor roles depending on the tumor microenvironment. In addition, IL-17A has been recently implicated in the recruitments of neutrophil into tumor tissues. Although IL-17A is considered tumor-promoting in breast cancer, its significance in the possible regulation of neutrophil infiltration in TNBC is not clearly defined. MATERIALS AND METHODS: We immunolocalized IL-17A, CD66b (neutrophil marker), and chemokine (C-X-C motif) ligand 1 (CXCL1, neutrophil chemoattractant) in 108 TNBC specimens and assessed their correlation among each other. The correlation between these markers and clinicopathological parameters was also assessed. We subsequently performed in vitro study to address the possible regulation of CXCL1 by IL-17A using TNBC cell lines, MDA-MB-231 and HCC-38. RESULTS: It was revealed that IL-17A correlated significantly with CXCL1 and CD66b, also CD66b with CXCL1. Furthermore, IL-17A was significantly associated with shorter disease-free and overall survival, especially in a high density CD66b group of patients. In vitro results revealed that IL-17A upregulated CXCL1 mRNA expression in a dose and time dependent manner, and this induction was significantly suppressed by an Akt inhibitor. CONCLUSION: IL-17A was considered to contribute to neutrophil infiltration by inducing CXCL1 in TNBC tissues and educating neutrophils to promote tumor progression. IL-17A might therefore serve as a potent prognostic factor in TNBC.

  30. Morphometric analysis of nuclear shape irregularity as a novel predictor of programmed death-ligand 1 expression in lung squamous cell carcinoma. International-journal

    Ryoko Saito-Koyama, Keiichi Tamai, Jun Yasuda, Yasunobu Okamura, Yuto Yamazaki, Chihiro Inoue, Yasuhiro Miki, Jiro Abe, Hisashi Oishi, Ikuro Sato, Hironobu Sasano

    Virchows Archiv : an international journal of pathology　2023/05/12

    DOI： 10.1007/s00428-023-03548-z 　

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    Immune checkpoint inhibitor (ICI) therapy has been established as one of the key treatment strategies for lung squamous cell carcinoma (LUSQ). The status of programmed death-ligand 1 (PD-L1) in tumor cells and/or immune cells using immunohistochemistry has been primarily used as a surrogate marker for determining ICI treatment; however, when the tissues to be examined are small, false-negative results could be unavoidable due to the heterogeneity of PD-L1 immunoreactivity. To overcome this practical limitation, we attempted to explore the status of nuclear atypia evaluated using morphometry as a potential predictor of PD-L1 status in LUSQ. We correlated the parameters related to nuclear atypia with PD-L1 status using two different cohorts of LUSQ patients (95 cases from The Cancer Genome Atlas database and 30 cases from the Miyagi Cancer Center). Furthermore, we studied the gene mutation status to elucidate the genetic profile of PD-L1 predictable cases. The results revealed that nuclear atypia, especially morphometric parameters related to nuclear shape irregularity, including aspect ratio, circularity, roundness, and solidity, were all significantly associated with PD-L1 status. Additionally, LUSQ cases with high PD-L1 expression and pronounced nuclear atypia were significantly associated with C10orf71 and COL14A1 mutations compared with those with low PD-L1 expression and mild nuclear atypia. We demonstrated for the first time that nuclear shape irregularity could represent a novel predictor of PD-L1 expression in LUSQ. Including the morphometric parameters related to nuclear atypia in conjunction with PD-L1 status could help determine an effective ICI therapeutic strategy; however, further investigation is required.

  31. Kallikrein-Related Peptidase 12 (KLK12) in Breast Cancer as a Favorable Prognostic Marker. International-journal

    Ai Sato, Kiyoshi Takagi, Ayano Yoshimura, Wakana Tsukamoto, Mio Yamaguchi-Tanaka, Yasuhiro Miki, Akiko Ebata, Minoru Miyashita, Takashi Suzuki

    International journal of molecular sciences　24　(9)　2023/05/08

    DOI： 10.3390/ijms24098419 　

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    Kallikrein-related peptides (KLKs) form an evolutionally conserved subgroup of secreted serine proteases that consists of 15 members (KLK1-15). Previous studies have shown that KLKs regulate diverse biological processes, but the clinical significance of KLKs remains largely unclear in human breast cancers. We examined the expression profile of 15 KLK genes in breast carcinomas using microarray data. Next, we immunolocalized KLK12 in 140 breast carcinomas and evaluated its clinical significance. Subsequently, we examined the effects of KLK12 on proliferation and migration in breast cancer cell lines. From microarray analyses, it turned out that KLK12 was the most strongly associated with low-grade malignancy in breast carcinomas among the 15 KLK members. Immunohistochemical KLK12 status was positively associated with ER and PR status, while it was inversely associated with stage, pathological T factor, lymph node metastasis, and distant metastasis. Prognostic analyses demonstrated that KLK12 was a favorable prognostic factor for both disease-free and breast cancer-specific survival of the patients. Furthermore, the knockdown of KLK12 significantly increased cell proliferation activity and cell migration of breast cancer cells. These results suggest that KLK12 has antitumorigenic effects associated with proliferation and migration and immunohistochemical KLK12 status as a potent favorable prognostic factor in breast carcinoma patients.

  32. PRB inhibited cell proliferation through let-7b-E2F1 in breast cancer. International-journal

    Teeranut Asavasupreechar, Ryoko Saito-Koyama, Yasuhiro Miki, Keiichi Tamai, Jiro Abe, Chihiro Inoue, Ikuro Sato, Viroj Boonyaratanakornkit, Hironobu Sasano

    Endocrine-related cancer　30　(7)　2023/05/01

    DOI： 10.1530/ERC-22-0204 　

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    The presence of progesterone receptor (PR) and PR isoform B (PRB) in breast cancer is generally correlated with better clinical outcomes. Additionally, the significance of hormone-independent effects of PR/PRB correlated with better prognosis have been reported in non-small lung cancer (NSCLC). However, the detailed mechanism of that still remains unclear. In this study, we examined how microRNAs (miRNAs) could contribute to tumor inhibition via PR/PRB expression, in order to find miRNAs that have tumor-agnostic effects between breast cancer and NSCLC. We obtained miRNAs data using human tissues of breast cancer and NSCLC from The Cancer Genome Atlas database and PCR array from NSCLC patients of our cohort. Subsequently, we examined the function of the miRNA through in vitro study using breast cancer cell lines. As a result, only let-7b expression was significantly correlated with PR expression in both cancers. Additionally, the expression of let-7b significantly inhibited cell proliferation by inducing PR and PRB expression in breast cancer cell lines. However, the positive correlation of let-7b and PRB required a mediated factor, E2F1, obtained from TGCA databases analysis. In vitro experiments showed that let-7b significantly inhibited E2F1, and E2F1 significantly inhibited PRB. This study revealed that PRB inhibits the proliferation of breast cancer cells by the let-7b-E2F1 interaction. In addition, the immunohistochemical analysis in NSCLC was also consistent with these in vitro data. Our results could contribute to developing novel therapeutic strategies for patients with PR/PRB-positive cancer by targeting let7b or PRB expression in breast cancer and possibly NSCLC.

  33. 乳癌におけるSGLT1の発現と予後不良因子としての検討

    角掛 聡子, 岩渕 英里奈, 三木 康宏, 小野寺 好明, 多田 寛, 原田 成美, 宮下 穣, 濱中 洋平, 江幡 明子, 柳垣 美歌, 進藤 晴彦, 本成 登貴和, 山崎 あすみ, 和田 基, 大沼 忍, 亀井 尚, 海野 倫明, 笹野 公伸, 鈴木 貴, 石田 孝宣

    日本外科学会定期学術集会抄録集　123回　DP-4　2023/04

    Publisher: (一社)日本外科学会

  34. The Pro-Tumorigenic Role of Chemotherapy-Induced Extracellular HSP70 from Breast Cancer Cells via Intratumoral Macrophages. International-journal

    Mio Yamaguchi-Tanaka, Kiyoshi Takagi, Yasuhiro Miki, Ai Sato, Erina Iwabuchi, Minoru Miyashita, Takashi Suzuki

    Cancers　15　(6)　2023/03/22

    DOI： 10.3390/cancers15061903 　

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    Tumor-associated macrophages (TAMs) contribute to tumor progression and chemoresistance; it is therefore important to clarify the altered functions of macrophages following chemotherapy. While extracellular heat shock protein (HSP) 70 is associated with therapeutic resistance, the effects of HSP70 on TAMs remain largely unknown. Here, we conducted in vitro experiments and immunohistochemistry in 116 breast carcinoma specimens to determine whether the secretion of HSP70 from breast cancer cells following chemotherapy affects macrophage function. It was revealed that the interaction of epirubicin (EPI)-exposed breast cancer cells with macrophages enhanced tumor progression, and EPI promoted the secretion of extracellular HSP70 from breast cancer cells. The expression of pro-tumorigenic macrophage marker CD163 was decreased in macrophages treated with a conditioned medium (CM) from HSP70-silenced breast cancer cells. Breast cancer cells treated with CM from HSP70-silenced breast cancer cells showed decreased expression of transforming growth factor (TGF)-β, and the pro-tumorigenic effects of macrophages were impaired when TGF-β signaling was inhibited. Immunohistochemistry demonstrated that HSP70 served as a poor prognostic factor in conjunction with macrophage infiltration. It was therefore concluded that extracellular HSP70 levels increased following chemotherapy and enhanced the pro-tumorigenic effects of TAMs, either directly or indirectly, by regulating TGF-β expression in breast cancer cells.

  35. 間質性肺炎における浸潤性粘液性腺癌発生機序の検討

    小山 涼子, 村上 圭吾, 村上 一宏, 三木 康宏, 井上 千裕, 田畑 俊治, 岡田 克典, 鈴木 貴, 鈴木 博義, 笹野 公伸

    日本病理学会会誌　112　(1)　330-330　2023/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  36. 肺腺癌間質における弾性線維、膠原線維の割合と癌関連線維芽細胞マーカーの発現に関する検討

    浅岡 祈里, 井上 千裕, 三木 康宏, 小山 涼子, 岡田 克典, 鈴木 貴

    日本病理学会会誌　112　(1)　385-385　2023/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  37. ステロイドホルモン研究における組織学的アプローチ

    鈴木貴, 山﨑有人, 岩渕英里奈, 横山敦, 髙木清司, 三木康宏

    日本組織細胞科学2023　197-206　2023

  38. In Situ Proximity Ligation Assay to Visualize Protein-Protein Interactions in Tumor Specimens. International-journal

    Yasuhiro Miki, Erina Iwabuchi, Takashi Suzuki

    Methods in molecular biology (Clifton, N.J.)　2660　123-135　2023

    DOI： 10.1007/978-1-0716-3163-8_9 　

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    Protein-protein interactions (PPI) are the basis of various biological phenomena, such as intracellular signal transduction, gene transcription, and metabolism. PPI are also considered to be involved in the pathogenesis and development of various diseases, including cancer. PPI phenomenon and their functions have been elucidated by gene transfection and molecular detection technologies. On the other hand, in histopathological analysis, although immunohistochemical analyses provide information pertaining to protein expression and their localization in pathophysiological tissues, it has been difficult to visualize the PPI of these proteins. An in situ proximity ligation assay (PLA) was developed as a microscopic visualization technique for PPI in formalin-fixed, paraffin-embedded (FFPE) tissues as well as in cultured cells and frozen tissues. PLA using histopathological specimens enables cohort studies of PPI, which can clarify the significance of PPI in pathology. We have previously shown the dimerization pattern of estrogen receptors and significance of HER2-binding proteins using breast cancer FFPE tissues. In this chapter, we describe a methodology for the visualization of PPI using PLA in pathological specimens.

  39. 間質性肺炎における浸潤性粘液性腺癌発癌予測因子の探索

    佐々木 祥人, 小山 涼子[齊藤], 村上 圭吾, 三木 康宏, 井上 千裕, 村上 一宏, 田畑 俊治, 岡田 克典, 鈴木 貴, 笹野 公伸

    肺癌　62　(6)　641-641　2022/11

    Publisher: (NPO)日本肺癌学会

    ISSN： 0386-9628

    eISSN： 1348-9992

  40. プロテオーム解析による間質性肺炎合併肺癌の線維性間質における新規治療マーカーの検索(Exploring therapeutic markers in fibrous stroma of lung cancer with usual interstitial pneumonia by proteomic analysis)

    井上 千裕, 三木 康宏, 小山 涼子, 岡田 克典, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　81回　P-1247　2022/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  41. 乳癌におけるリガンド誘導体染色によるソマトスタチン受容体の検出(Detection of somatostatin receptors using ligand derivative staining in breast cancer)

    岩渕 英里奈, 飯田 謙人, 三木 康宏, 長谷川 功紀, 深澤 匠, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　81回　P-1316　2022/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  42. GPERとERβの影響がトリプルネガティブ乳癌に与える影響(Effects of G protein-coupled estrogen receptor and estrogen receptor β on triple-negative breast cancer)

    深澤 匠, 岩渕 英里奈, 三木 康宏, 長谷川 功紀, 小野寺 好明, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　81回　J-3023　2022/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  43. 乳癌におけるRHAMM(ヒアルロン酸媒介運動性受容体)の発現意義(The role of RHAMM(receptor for hyaluronan-mediated motility) in human breast carcinoma)

    藤沢 詩織, 高木 清司, 山口 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 多田 寛, 鈴木 貴, 石田 孝宣

    日本癌学会総会記事　81回　P-3244　2022/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  44. 化学療法誘導性細胞外HSP70はマクロファージの腫瘍促進作用を介して乳癌の進展を促進する(Chemotherapy-induced extracellular HSP70 enhances the ability of marcophages to promote breast cancer progression.)

    山口 美桜, 高木 清司, 三木 康宏, 岩渕 英里奈, 宮下 穣, 鈴木 貴

    日本癌学会総会記事　81回　P-3245　2022/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  45. 化学療法後の乳癌細胞は細胞外HSP70の分泌を介してマクロファージの腫瘍促進作用を誘導する

    山口 美桜, 高木 清司, 三木 康宏, 岩渕 英里奈, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本乳癌学会総会プログラム抄録集　30回　EP1-14　2022/06

    Publisher: (一社)日本乳癌学会

  46. FE65 defines the efficacy of tamoxifen treatment via osteopontin expression in estrogen receptor-positive breast cancer. International-journal

    Junyao Xu, Erina Iwabuchi, Yasuhiro Miki, Ayako Kanai, Kiyoshi Takagi, Takashi Suzuki, Takanori Ishida, Hironobu Sasano

    Pathology, research and practice　234　153898-153898　2022/06

    DOI： 10.1016/j.prp.2022.153898 　

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    BACKGROUND: Metastasis and endocrine therapy resistance are clinical challenges in the treatment of estrogen receptor (ER) -positive breast tumors. Therefore, mechanistic exploration of tamoxifen (TAM) resistance is considered pivotal to improve the prognosis of ER positive breast cancer patients. We previously demonstrated the correlation between FE65 and ER, and subsequently explored the effects of FE65 on TAM and potential interaction between FE65 and Osteopontin (OPN) in ER-positive breast cancer. METHODS: We immunolocalized FE65 and OPN in ER-positive breast cancers and correlated the results with their clinicopathological variables. We then performed proximity ligation and proliferation assays to correlate TAM resistance with FE65 expression. The RT2 Profiler Human PCR Array Human Estrogen Receptor Signaling was also used to profile 96 ER related genes. Hoechst 33342 Staining was used to evaluate apoptosis. RESULTS: FE65 immunoreactivity was significantly associated with higher pathological N factor of the cases examined, and a potential correlation with tamoxifen resistance of the ER-positive patients. FE65 knockdown significantly increased the proportion of apoptotic carcinoma cells. The statistically significant positive correlation between FE65 and OPN was detected in this study. Subsequent immunohistochemical analyses revealed that OPN status was significantly associated with cancer metastasis and overall survival of 142 patients and FE65 status. CONCLUSIONS: We firstly demonstrated the clinicopathological significance of FE65 in ER-positive breast cancer patients and results indicated that the effects of FE65 on ER-positive breast cancer patients were mediated through OPN expression. In addition, results suggested the clinical value of FE65 as potential prognostic factor and surrogate marker of TAM therapy in ER-positive breast cancer patients.

  47. トリプルネガティブ乳癌組織における性ホルモン濃度の検討

    江幡 明子, 三木 康宏, 高木 清司, 岩渕 英里奈, 多田 寛, 原田 成美, 濱中 洋平, 宮下 穣, 佐藤 章子, 深町 佳世子, 金井 綾子, 柳垣 美歌, 進藤 晴彦, 角掛 聡子, 本成 登貴和, 大沼 忍, 亀井 尚, 海野 倫明, 鈴木 貴, 石田 孝宣

    日本外科学会定期学術集会抄録集　122回　DP-8　2022/04

    Publisher: (一社)日本外科学会

  48. アンドロゲンによる乳癌組織随伴マクロファージの悪性形質顕在化メカニズムの解明

    山口 美桜, 高木 清司, 三木 康宏, 小野寺 好明, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　97　(5)　1624-1624　2022/03

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  49. Appropriate Health Management Considering the Vulnerability of Women during Disasters.

    Yasuhiro Miki, Kiyoshi Ito

    The Tohoku journal of experimental medicine　256　(3)　187-195　2022/03

    DOI： 10.1620/tjem.256.187 　

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    In situations of a disaster, it has been observed that the damage suffered by women and men is not equal. The vulnerability of women during disasters has been the focus of several studies and disaster management guidelines. Records show that there were more women victims than men victims in both the Great Hanshin-Awaji Earthquake in 1995 and the Great East Japan Earthquake (GEJE) in 2011. Biologically speaking, women are physically less fit than men are; hence, they are more susceptible to physical disabilities induced by disasters and may be disadvantaged in evacuation situations. However, vulnerability of women during disasters is a complex problem that involves physical fitness, as well as other various factors. In the Sendai Framework for Disaster Risk Reduction 2015-2030 (SFDRR) adopted in 2015, prioritized actions such as "Build Back Better" were defined based on the GEJE experiences. In the SFDRR, in addition to vulnerability of women during disasters, medical services including maternal, newborn, and child health and sexual and reproductive health are considered the key factors for disaster risk reduction. This has been discussed in all phases of disaster risk reduction planning and post-disaster response. These findings suggest that the role of obstetrics and gynecology is comprehensive and important as a part of disaster medicine at the local and national levels, as recommended in the SFDRR. In this review, we summarized the management of women's health and gynecological responses during disasters and considered the importance of women as stakeholders in disaster risk reduction.

  50. Co-expression of nuclear heterogeneous nuclear ribonucleic protein K and estrogen receptor α in endometrial cancer International-journal

    Yasuhiro Miki, Erina Iwabuchi, Kiyoshi Takagi, Takashi Suzuki, Hironobu Sasano, Nobuo Yaegashi, Kiyoshi Ito

    Pathology - Research and Practice　231　153795-153795　2022/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.prp.2022.153795 　

    ISSN： 0344-0338

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    Heterogeneous nuclear ribonucleic protein K (hnRNPK) regulates the expression of various genes, but has contradictory roles as a tumor promoter and a tumor suppressor. We recently reported that the expression of hnRNPK is negatively associated with malignant behavior of breast cancer where it was induced by estrogen, and bound to estrogen receptor α (ERα) in the nucleus of breast cancer cells. However, the significance of hnRNPK in endometrial cancer, also an estrogen-dependent cancer, remains unclear. In this study, we first examined the localization of hnRNPK and ERα in normal endometrium and endometrial cancer. hnRNPK and ERα immunoreactivity was detected in the nuclei of endometrial glandular and carcinoma cells. In normal endometria, hnRNPK labeling index/immuno-intensity was significantly higher in the proliferative phase than in the secretory phase. In endometrial cancer tissues, hnRNPK labeling index/immuno-intensity was significantly higher in the adjacent non-malignant glandular cells compared to that in carcinoma cells. Immunohistochemistry results for ERα were identical to that of hnRNPK both in normal endometrium and endometrial cancer. In normal and cancerous tissues, the median value of the hnRNPK labeling index was significantly higher in the ERα-high group. Intratumoral estrogen, but not androgen, measured using liquid chromatography-tandem mass spectrometry, was significantly positively correlated with the hnRNPK labeling index in endometrial cancer tissues. Database analysis revealed that the hnRNPK high expression group had a significantly better prognosis for both overall and disease-free survival. These results suggest that hnRNPK interacts with ERα to regulate endometrial changes during the menstrual cycle and suppress the malignant behavior of endometrial cancer.

  51. Immunolocalization of CD80 and CD86 in Non-Small Cell Lung Carcinoma: CD80 as a Potent Prognostic Factor.

    Takashi Sato, Kiyoshi Takagi, Mitsunori Higuchi, Hiroko Abe, Michie Kojimahara, Miho Sagawa, Megumi Tanaki, Yasuhiro Miki, Takashi Suzuki, Hiroshi Hojo

    Acta histochemica et cytochemica　55　(1)　25-35　2022/02/26

    DOI： 10.1267/ahc.21-00075 　

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    It has been demonstrated that tumor cells express programed cell death protein 1 (PD-L1) to escape T lymphocytes that express programed cell protein 1 (PD-1), and PD-1/PD-L1 immune checkpoint inhibitors have been regarded in lung cancer patients. CD80 and CD86 are members of B7 superfamily which regulates T lymphocyte activation and tolerance. However, immunolocalization of CD80 and CD86 has not been examined in the lung carcinoma tissues and their clinical significance remains unknown. Therefore, to clarify clinical significance of CD80 and CD86, we immunolocalized these in 75 non-small cell lung carcinomas (NSCLC) in this study. Immunoreactivities of CD80 and CD86 were mainly detected in tumor-infiltrating macrophages. Immunohistochemical CD80 status was high in 56% of NSCLC, and it was positively associated with stage, pathological T factor, distant metastasis, histological type and PD-L1 status. Moreover, multivariate analysis turned out that the CD80 status was an independent worse prognostic factor. CD86 status was high in 53% of the cases, but it was not significantly associated with any clinicopathological parameters. These findings suggest that CD80 is a potent worse prognostic factor possibly in association with escape from immune attack in NSCLC.

  52. Vasohibin-1 and -2 in pulmonary lymphangioleiomyomatosis (LAM) cells associated with angiogenic and prognostic factors International-journal

    Chihiro Inoue, Yasuhiro Miki, Ryoko Saito-Koyama, Kazuma Kobayashi, Kuniaki Seyama, Yoshinori Okada, Hironobu Sasano

    Pathology - Research and Practice　230　153758-153758　2022/02

    Publisher: Elsevier BV

    DOI： 10.1016/j.prp.2022.153758 　

    ISSN： 0344-0338

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    Lymphangioleiomyomatosis (LAM) is a rare pulmonary neoplasm, clinically associated with dyspnea and respiratory failure. Current therapeutic modalities do not necessarily reach satisfactory outcome and novel therapeutic approaches are currently warranted. Therefore, in this study, we focused on vasohibin-1 (VASH1) and -2 (VASH2); VASH1 terminated and VASH2 promoted angiogenesis. In addition, both VASH1/2 were reported to influence the progression of various human malignancies. We first performed hierarchical clustering analysis to attempt to classify 36 LAM cases into three different clusters according to immunoreactivity of VASH1/2 and other angiogenic and prognostic factors of LAM; VEGFR1/2/3, p-mTOR, p-S6, p-4EBP, ERα, PgR, MMP2, and MMP9. The cluster harboring higher angiogenic factors had higher VASH1/2 status. VASH1 was significantly positively correlated with VEGFR2, MMP9, and p-mTOR (p-value <0.05), and VASH2 with both angiogenic and prognostic factors including VEGFR1, PgR, MMP9, p-mTOR, p-S6, and p-4EBP (p-value <0.05). Subsequent PCR array of angiogenic genes demonstrated that high VASH1 mRNA was significantly positively associated with the status of SPHK1 and TYPM, lower EGF and EFNB2 (p-value <0.05), and high VASH2 mRNA negatively with MMP2 (p-value <0.05). VASH1 was considered to be up-regulated by activation of angiogenesis, whereas VASH2 could influence the angiogenesis and progression of LAM.

  53. Visualization of the protein-protein interactions of hormone receptors in hormone-dependent cancer research. International-journal

    Erina Iwabuchi, Yasuhiro Miki, Takashi Suzuki, Hironobu Sasano

    Endocrine oncology (Bristol, England)　2　(1)　R132-R142　2022/01

    DOI： 10.1530/EO-22-0059 　

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    In hormone-dependent cancers, the activation of hormone receptors promotes the progression of cancer cells. Many proteins exert their functions through protein-protein interactions (PPIs). Moreover, in such cancers, hormone-hormone receptor binding, receptor dimerization, and cofactor mobilization PPIs occur primarily in hormone receptors, including estrogen, progesterone, glucocorticoid, androgen, and mineralocorticoid receptors. The visualization of hormone signaling has been primarily reported by immunohistochemistry using specific antibodies; however, the visualization of PPIs is expected to improve our understanding of hormone signaling and disease pathogenesis. Visualization techniques for PPIs include Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation analysis; however, these techniques require the insertion of probes in the cells for PPI detection. Proximity ligation assay (PLA) is a method that could be used for both formalin-fixed paraffin-embedded (FFPE) tissue as well as immunostaining. It can also visualize hormone receptor localization and post-translational modifications of hormone receptors. This review summarizes the results of recent studies on visualization techniques for PPIs with hormone receptors; these techniques include FRET and PLA. In addition, super-resolution microscopy has been recently reported to be applicable to their visualization in both FFPE tissues and living cells. Super-resolution microscopy in conjunction with PLA and FRET could also contribute to the visualization of PPIs and subsequently provide a better understanding of the pathogenesis of hormone-dependent cancers in the future.

  54. Correction to: Breast cancer, diabetes mellitus and glucagon‑like peptide‑1 receptor toward exploring their possible associations. International-journal

    Naoko Hashimoto Takigami, Shimpei Kuniyoshi, Yasuhiro Miki, Kentaro Tamaki, Yoshihiko Kamada, Kano Uehara, Seiko Tsuchiya, Shigeharu Terukina, Erina Iwabuchi, Ayako Kanai, Minoru Miyashita, Takanori Ishida, Nobumitsu Tamaki, Hironobu Sasano

    Breast cancer research and treatment　191　(1)　221-221　2022/01

    DOI： 10.1007/s10549-021-06380-8 　

  55. B7-1 and programmed cell death-ligand 1 in primary and lymph node metastasis lesions of non-small cell lung carcinoma. International-journal

    Takehiro Yamada, Yasuhiro Miki, Miho Suzuki, Osamu Kondoh, Ryoko Saito-Koyama, Katsuhiko Ono, Yoshinori Okada, Hironobu Sasano

    Cancer medicine　11　(2)　479-491　2022/01

    DOI： 10.1002/cam4.4444 　

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    BACKGROUND: Programmed cell death ligand 1 (PD-L1) status has been reported to be different between metastatic and primary lesions in some cases. Therefore, the interaction between carcinoma and immune cells could influence their expression in the tumor microenvironment. PD-L1 is known to bind not only to Programmed cell death 1 (PD-1) but also to B7-1 (CD80). In this study, we examined the interaction between lung carcinoma cell lines and peripheral blood mononuclear cells (PBMCs) in vitro. We then examined the significance of B7-1 expression non-small cell lung cancer (NSCLC) microenvironment. METHODS: The interaction of lung carcinoma cell lines and PBMC through the soluble factors was analyzed using a co-culture system. The changes in expression of immune checkpoint-related factors in PBMC were examined by PD-1/PD-L1 Checkpoint Pathway qPCR Array Kit. B7-1 expression in NSCLC tissues was examined by immunohistochemistry. RESULTS: B7-1 was upregulated following the co-culture with the lung carcinoma cell lines. B7-1 expression in NSCLC tissues was significantly higher in smokers and squamous cell carcinomas and was significantly positively correlated with PD-L1 status in primary cancer. However, B7-1 and PD-1 were not correlated between primary and metastatic diseases in the same patients. CONCLUSION: PD-1 inhibitors inhibited PD-1/PD-L1 binding but not PD-L1/B7-1 binding. These results demonstrated that the intratumoral ratio of B7-1 positive T cells in NSCLC tissue could be involved in the therapeutic efficacy of PD-L1 inhibitors. This study focused on lymph node metastasis but other sites of distant metastases should be explored by further analysis.

  56. The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination.

    Erina Iwabuchi, Yasuhiro Miki, Hironobu Sasano

    Acta histochemica et cytochemica　54　(6)　177-183　2021/12/24

    DOI： 10.1267/ahc.21-00084 　

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    The therapeutic strategy is determined by protein expression using immunohistochemistry of estrogen receptor (ER), progesterone receptor, and human epidermal growth factor receptor 2 (HER2) in formalin-fixed paraffin-embedded (FFPE) breast cancer tissues. However, few proteins function independently, and many of them functions due to protein-protein interactions (PPIs) with other proteins. Therefore, it is important to focus on PPIs. This review summarizes the PPIs of ER and HER2 in breast cancer, especially those using a proximity ligation assay that can visualize PPIs in FFPE tissues. In particular, assessing the interaction of CEACAM6 with HER2 may serve as a surrogate marker for the efficacy of trastuzumab in patients with breast cancer. Therefore, in this review, the technique used to detect the interaction of CEACAM6 and HER2 in routinely processed pathological specimens will be applied to the clinical practice of drug selection. We showed the possibility as a novel pathological examination method using PPIs.

  57. FE65 in breast cancer and its clinicopathological significance.

    Junyao Xu, Erina Iwabuchi, Yasuhiro Miki, Ayako Kanai, Takanori Ishida, Hironobu Sasano

    Breast cancer (Tokyo, Japan)　29　(1)　144-155　2021/09/08

    DOI： 10.1007/s12282-021-01291-4 　

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    BACKGROUND: Transcription coregulator adapter protein FE65 is well known to play pivotal roles in pathogenesis of Alzheimer's disease by regulating amyloid precursor protein (APP) expression and processing. APP was recently reported to be also involved in development of human malignancies. Therefore, in this study, we studied FE65 status in different subtypes of human breast cancer and correlated the results with cell proliferation and migration of carcinoma cells and clinicopathological features of breast cancer patients to explore its biological and clinical significance in breast cancer. METHODS: We first immunolocalized FE65 and APP in 138 breast cancer patients and correlated the results with their tumor grade. Then, we did further exploration by proximity ligation assay, WST-8, and wound-healing assay. RESULTS: FE65 immunoreactivity in carcinoma cells was significantly associated with lymph-node metastasis, ERα, and high pathological N factor. APP immunoreactivity was significantly positively correlated with high pathological N factor. FE65, APP, and p-APP were all significantly correlated with shorter disease-free survival of breast cancer patients. In addition, the status of FE65 was significantly associated with overall survival. Results of in vitro analysis revealed that FE65 promoted the migration and proliferation of T-47D and ZR-75-1 breast carcinoma cells. In situ proximity ligation assay revealed that FE65 could bind to APP in the cytoplasm. FE65 was also associated with APP and ERα in carcinoma cells, suggesting their cooperativity in promoting carcinoma cell proliferation and migration. APP was also significantly associated with adverse clinical outcome of the patients. CONCLUSIONS: This is the first study to explore the clinical significance of FE65 in human breast cancer. The significant positive correlation of FE65 with poor clinical outcome, direct binding to APP, and promotion of carcinoma cell proliferation and migration indicated that FE65-APP pathway could serve as the potential candidate of therapeutic intervention in breast cancer patients.

  58. 子宮内膜癌におけるhnRNPKとエストロゲン受容体αの発現

    三木 康宏, 岩渕 英里奈, 高木 清司, 鈴木 貴, 笹野 公伸, 伊藤 潔

    日本癌学会総会記事　80回　[P13-2]　2021/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  59. 乳癌細胞における化学療法誘導性エクソソーム内包HSP70はマクロファージの腫瘍促進作用を誘導する

    山口 美桜, 高木 清司, 三木 康宏, 岩渕 英里奈, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　80回　[P14-5]　2021/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  60. Androgens enhance the ability of intratumoral macrophages to promote breast cancer progression. International-journal

    Mio Yamaguchi, Kiyoshi Takagi, Masayasu Sato, Ai Sato, Yasuhiro Miki, Yoshiaki Onodera, Minoru Miyashita, Hironobu Sasano, Takashi Suzuki

    Oncology reports　46　(3)　2021/09

    DOI： 10.3892/or.2021.8139 　

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    Androgens are produced locally in breast carcinoma tissues by androgen‑producing enzymes such as 5α‑reductase type 1 (5αRed1) and affect not only breast cancer cells but the tumor microenvironment as well. Tumor‑associated macrophages (TAMs) are primary components of the tumor microenvironment and contribute to tumor progression. Although previous studies suggest that androgen/androgen receptor (AR) signaling in macrophages has important roles in human diseases, androgen action on TAMs has remained largely unknown. We immunolocalized macrophage marker CD163 as well as AR and 5αRed1 in 116 breast carcinomas and correlated them with clinicopathological parameters and clinical outcomes. Moreover, we examined the roles of androgens on macrophages in breast cancer progression using cell lines 4T1 (mouse breast cancer) and RAW264.7 (macrophage) in a tumor‑bearing female BALB/c mouse model. Double immunohistochemistry revealed that AR was sporadically expressed in the macrophages in breast carcinoma tissues. Macrophage infiltration was significantly correlated with an aggressive phenotype of breast carcinomas and worse prognosis, especially in the 5αRed1‑positive group. In a sphere‑forming assay using 4T1 and RAW‑AR cells, which stably express AR, the sphere size was significantly increased due to androgens when 4T1 cells were cocultured with RAW‑AR cells. Furthermore, in vivo experiments revealed that tumor growth and Ki67, a cell proliferation marker, were increased when androgens were stably produced in breast cancer cells and AR was expressed in macrophages. In conclusion, AR is expressed in intratumoral macrophages and is associated with an aggressive phenotype of breast carcinomas, especially when breast cancer cells actively produce androgens. Thus, androgens may enhance the ability of macrophages to promote breast cancer progression.

  61. Forkhead Box I1 in Breast Carcinoma as a Potent Prognostic Factor.

    Yoshiaki Onodera, Kiyoshi Takagi, Yoshimi Neoi, Ai Sato, Mio Yamaguchi, Yasuhiro Miki, Akiko Ebata, Minoru Miyashita, Hironobu Sasano, Takashi Suzuki

    Acta histochemica et cytochemica　54　(4)　123-130　2021/08/25

    DOI： 10.1267/ahc.21-00034 　

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    Forkhead box (FOX) proteins are family of transcriptional factors and regulate cell growth and differentiation as well as embryogenesis and longevity. Previous studies have demonstrated that several FOX members regulate growth or metastasis of breast carcinoma, but clinical significance of total FOX members remains unclear. We first examined associations between expression of 40 FOX genes and TNM status of 19 breast carcinoma using microarray data. Subsequently, we immunolocalized FOXI1 in 140 breast carcinomas and evaluated its clinicopathological significance. In the microarray analysis, we newly identified that gene expression of FOXI1 was most pronouncedly linked to metastasis of the breast carcinoma among the FOX members examined. However, clinicopathological significance of FOXI1 has not been examined in the breast carcinoma. FOXI1 immunoreactivity was positive in 44 out of 140 (31%) of breast carcinomas, and it was significantly associated with stage, lymph node metastasis and distant metastasis. The FOXI1 status was significantly associated with worse prognosis of the breast cancer patients, and it turned out to be an independent prognostic factor for both distant disease-free survival and breast cancer-specific survival. These findings suggest that FOXI1 plays important roles in the metastasis of breast carcinoma and immunohistochemical FOXI1 status is a potent prognostic factor.

  62. EphB4 as a Novel Target for the EGFR-Independent Suppressive Effects of Osimertinib on Cell Cycle Progression in Non-Small Cell Lung Cancer. International-journal

    Ren Nanamiya, Ryoko Saito-Koyama, Yasuhiro Miki, Chihiro Inoue, Teeranut Asavasupreechar, Jiro Abe, Ikuro Sato, Hironobu Sasano

    International journal of molecular sciences　22　(16)　2021/08/07

    DOI： 10.3390/ijms22168522 　

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    Osimertinib is the latest generation epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor used for patients with EGFR-mutated non-small cell lung cancer (NSCLC). We aimed to explore the novel mechanisms of osimertinib by particularly focusing on EGFR-independent effects, which have not been well characterized. We explored the EGFR-independent effects of osimertinib on cell proliferation using NSCLC cell lines, an antibody array analysis, and the association between the action of osimertinib and the ephrin receptor B4 (EphB4). We also studied the clinicopathological significance of EphB4 in 84 lung adenocarcinoma patients. Osimertinib exerted significant inhibitory effects on cell growth and cell cycle progression by promoting the phosphorylation of p53 and p21 and decreasing cyclin D1 expression independently of EGFR. EphB4 was significantly suppressed by osimertinib and promoted cell growth and sensitivity to osimertinib. The EphB4 status in carcinoma cells was positively correlated with tumor size, T factor, and Ki-67 labeling index in all patients and was associated with poor relapse-free survival in EGFR mutation-positive patients. EphB4 is associated with the EGFR-independent suppressive effects of osimertinib on cell cycle and with a poor clinical outcome. Osimertinib can exert significant growth inhibitory effects in EGFR-mutated NSCLC patients with a high EphB4 status.

  63. Breast Cancer, Diabetes Mellitus and Glucagon-Like Peptide-1 Receptor Toward Exploring Their Possible Associations. International-journal

    Naoko Hashimoto Takigami, Shimpei Kuniyoshi, Yasuhiro Miki, Kentaro Tamaki, Yoshihiko Kamada, Kano Uehara, Seiko Tsuchiya, Shigeharu Terukina, Erina Iwabuchi, Ayako Kanai, Minoru Miyashita, Takanori Ishida, Nobumitsu Tamaki, Hironobu Sasano

    Breast cancer research and treatment　189　(1)　39-48　2021/08

    DOI： 10.1007/s10549-021-06288-3 　

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    PURPOSE: Diabetes Mellitus (DM) has been one of the well known risk factors of breast cancer (BC) development and also associated with adverse clinical outcomes of BC patients. Glucagon-like peptide-1 (GLP-1) receptor agonists have been used as antidiabetic therapeutic agents and recent epidemiological studies have reported their use to be correlated with increased BC risks. However, biological or pathological details have remained unknown. Therefore, in this study, we examined the status of GLP-1 receptor (GLP-1R) in BC with and without DM and correlated the findings with the clinicopathological factors of the patients to explore the possible involvement of GLP-1 in BC pathology. METHODS: We immunolocalized GLP-1R in cancer and adjacent non-pathological breast tissues in BC patients with DM (125 cases) and without DM (58 cases). We then compared the status of GLP-1R with that of fibroblast growth factor 7 (FGF7) and fibroblast growth factor receptor 2 (FGFR2), Ki-67 labeling index (Ki-67 LI) and disease free survival (DFS) of the patients and also between cancerous and non-pathological breast tissues. RESULTS: GLP-1R immunoreactivity was significantly higher (p = 0.044) in the patients with DM than without in carcinoma tissues. However, this was detected only in invasive carcinoma (p < 0.01) and not in non-invasive carcinoma nor non-pathological mammary glands. FGF7 was significantly correlated with the status of GLP-1R in BC (p = 0.045). In addition, in ER positive BC cases, those with GLP-1R positive status tended to have higher Ki-67 LI of more than 14% (p = 0.070). CONCLUSION: These findings all demonstrated the possible association between GLP-1R status and biological features of BC, especially of invasive BC in DM patients.

  64. D-2-hydroxyglutarate dehydrogenase in breast carcinoma as a potent prognostic marker associated with proliferation. International-journal

    Chiaki Hayashi, Kiyoshi Takagi, Ai Sato, Mio Yamaguchi, Hiroyuki Minemura, Yasuhiro Miki, Narumi Harada-Shoji, Minoru Miyashita, Hironobu Sasano, Takashi Suzuki

    Histology and histopathology　36　(10)　18362-18362　2021/07/23

    DOI： 10.14670/HH-18-362 　

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    BACKGROUND: D-2-hydroxyglutarate dehydrogenase (D2HGDH) catalyzes D-2-hydroxyglutarate to α-ketoglutarate and is involved in the regulation of cellular energy and biosynthetic intermediates. Previously, D2HGDH was reported to decrease 2-hydroxyglutarate level in breast carcinoma cells, but no other report has examined D2HGDH in breast carcinoma, and its significance remains unknown. METHODS: We first immunolocalized D2HGDH in 224 invasive breast carcinomas and evaluated its clinicopathological significance. We next examined associations between gene expression of D2HGDH and α-ketoglutarate-dependent dioxygenases in 23 breast carcinoma tissues using the gene expression profile data. Finally, we examined the effects of D2HGDH on the proliferation in three breast carcinoma cells. RESULTS: D2HGDH immunoreactivity was detected in 49% of invasive breast carcinomas, and the immunohistochemical D2HGDH status was positively associated with histological grade, HER2 and Ki-67, while it was inversely associated with estrogen receptor. Moreover, it was significantly associated with worse prognosis of the breast cancer patients, and it turned out to be an independent prognostic factor for both the disease-free and breast cancer-specific survival in these patients. Gene expression profile data revealed that D2HGDH expression was positively associated with the expression of 6 α-ketoglutarate-dependent dioxygenases (KDM3A, PLOD1, EGLN2, ALKBH1, ASPH and ALKBH7). Consequent in vitro experiments demonstrated that D2HGDH overexpression significantly increased the cell proliferation activity of MCF-7, T47D and MDA-MB-231 cells. CONCLUSION: These results suggest that D2HGDH plays an important role in the growth of breast carcinoma, possibly through regulating functions of α-ketoglutarate-dependent dioxygenases, and that D2HGDH status is a potent worse prognostic factor in breast cancer patients.

  65. Isoforms of IDH in breast carcinoma: IDH2 as a potent prognostic factor associated with proliferation in estrogen-receptor positive cases.

    Hiroyuki Minemura, Kiyoshi Takagi, Ai Sato, Mio Yamaguchi, Chiaki Hayashi, Yasuhiro Miki, Narumi Harada-Shoji, Minoru Miyashita, Hironobu Sasano, Takashi Suzuki

    Breast cancer (Tokyo, Japan)　28　(4)　915-926　2021/07

    DOI： 10.1007/s12282-021-01228-x 　

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    BACKGROUND: Isocitrate dehydrogenase (IDH) is an important enzyme that oxidatively decarboxylates isocitrate to α-ketoglutarate, and three isoforms (IDH1-3) have been identified. Overexpression and/or downregulation of IDH isoforms was reported in several human malignancies, suggesting importance of IDH in oncogenesis. However, significance of IDH isoforms remains largely unclear in the breast carcinoma. METHODS: We immunolocalized IDH1, IDH2 and IDH3α in 226 breast carcinomas and evaluated their clinical significance. Subsequently, we examined effects of IDH2 on proliferation in breast carcinoma cells. RESULTS: Immunoreactivity of IDH1-3α was detected in 53%, 38% and 41% of breast carcinomas, and the non-neoplastic epithelium was IDH1-positive, IDH2-negative and IDH3α-positive. IDH1 immunoreactivity was inversely associated with pathological T factor (pT) and Ki-67 in the breast carcinoma, while IDH3α immunoreactivity was not significantly associated with clinicopathological factors. IDH2 status was positively correlated with stage, pT, histological grade, HER2, Ki-67 and microvessel density. Moreover, IDH2 status was significantly associated with worse prognosis of the patients, and it turned out an independent prognostic factor for estrogen-receptor (ER) positive patients. These findings were more evident in the IDH1-negative / IDH2-positive/IDH3α-negative subgroup which is the opposite immunohistochemical IDH phenotype of normal mammary epithelium. In vitro studies demonstrated that RNA interference of IDH2 significantly decreased proliferation activity of T47D and SKBR-3 cells. CONCLUSION: These results suggest that IDH2 is associated with an aggressive phenotype of breast carcinoma through increasing cell proliferation, different from IDH1 and IDH3α, and immunohistochemical IDH2 status is a potent prognostic factor especially in ER-positive breast cancer patients.

  66. Estrogen Receptor β Is Involved in Acquired Resistance to EGFR-tyrosine Kinase Inhibitors in Lung Cancer. International-journal

    Hiroki Sugiura, Yasuhiro Miki, Erina Iwabuchi, Ryoko Saito, Katsuhiko Ono, Ikuro Sato, Yoshinori Okada, Hironobu Sasano

    Anticancer research　41　(5)　2371-2381　2021/05

    DOI： 10.21873/anticanres.15012 　

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    BACKGROUND/AIM: Acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) has posed serious clinical problems in the treatment of lung adenocarcinoma (LADC) patients harboring relevant EGFR mutations. In this study, we explored the role of estrogen receptor β (ERβ) in the development of acquired resistance to EGFR-TKIs in human LADC. MATERIALS AND METHODS: First, the role of ERβ in erlotinib resistance of LADC cell lines (PC9/ER) was examined. Then, the immunolocalization of ERβ in 28 LADC patient samples treated with EGFR-TKIs was investigated. RESULTS: Cytoplasmic ERβ was upregulated in erlotinib resistant cell lines. EGFR-TKIs sensitivity increased with ERβ inhibition in PC9/ER cells. ERK1/2 and AKT activities were both markedly increased by specific ERβ agonists even under erlotinib treatment of PC9/ER cells. Cytoplasmic ERβ immunoreactivity was significantly associated with clinical response to EGFR-TKIs. CONCLUSION: Cytoplasmic ERβ in LADC cells was involved in the development of resistance to EGFR-TKIs.

  67. 子宮内膜癌における組織中ステロイドホルモン濃度とTリンパ球サブタイプ

    三木 康宏, 大沼 楓佳, 高木 清司, 岩渕 英里奈, 鈴木 貴, 笹野 公伸, 伊藤 潔

    日本内分泌学会雑誌　96　(4)　1041-1041　2021/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  68. 前立腺癌におけるRac1およびARHGAP15の発現意義

    佐藤 和, 高木 清司, 佐藤 真彦, 三木 康宏, 伊藤 明宏, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　96　(4)　1080-1080　2021/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  69. 子宮内膜癌におけるDehydroepiandrosteroneの直接作用に関する検討

    三木 康宏, 高木 清司, 鈴木 貴, 伊藤 潔

    日本内分泌学会雑誌　96　(4)　1080-1080　2021/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  70. Heterogeneous Nuclear Ribonucleoprotein K Is Involved in the Estrogen-Signaling Pathway in Breast Cancer. International-journal

    Erina Iwabuchi, Yasuhiro Miki, Takashi Suzuki, Hisashi Hirakawa, Takanori Ishida, Hironobu Sasano

    International journal of molecular sciences　22　(5)　2021/03/04

    DOI： 10.3390/ijms22052581 　

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    Heterogeneous nuclear ribonucleoprotein K (hnRNPK) transcripts are abundant in estrogen receptor (ER)- or progesterone receptor (PR)-positive breast cancer. However, the biological functions of hnRNPK in the ER-mediated signaling pathway have remained largely unknown. Therefore, this study analyzes the functions of hnRNPK expression in the ER-mediated signaling pathway in breast cancer. We initially evaluated hnRNPK expression upon treatment with estradiol (E2) and ICI 182,780 in the ERα-positive breast carcinoma cell line MCF-7. The results revealed that E2 increased hnRNPK; however, hnRNPK expression was decreased with ICI 182,780 treatment, indicating estrogen dependency. We further evaluated the effects of hnRNPK knockdown in the ER-mediated signaling pathway in MCF-7 cells using small interfering RNAs. The results revealed that hnRNPK knockdown decreased ERα expression and ERα target gene pS2 by E2 treatment. As hnRNPK interacts with several other proteins, we explored the interaction between hnRNPK and ERα, which was demonstrated using immunoprecipitation and proximity ligation assay. Subsequently, we immunolocalized hnRNPK in patients with breast cancer, which revealed that hnRNPK immunoreactivity was significantly higher in ERα-positive carcinoma cells and significantly lower in Ki67-positive or proliferative carcinoma cells. These results indicated that hnRNPK directly interacted with ERα and was involved in the ER-mediated signaling pathway in breast carcinoma. Furthermore, hnRNPK expression could be an additional target of endocrine therapy in patients with ERα-positive breast cancer.

  71. Stromal CCL5 Promotes Breast Cancer Progression by Interacting with CCR3 in Tumor Cells. International-journal

    Mio Yamaguchi, Kiyoshi Takagi, Koki Narita, Yasuhiro Miki, Yoshiaki Onodera, Minoru Miyashita, Hironobu Sasano, Takashi Suzuki

    International journal of molecular sciences　22　(4)　2021/02/15

    DOI： 10.3390/ijms22041918 　

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    Chemokines secreted from stromal cells have important roles for interactions with carcinoma cells and regulating tumor progression. C-C motif chemokine ligand (CCL) 5 is expressed in various types of stromal cells and associated with tumor progression, interacting with C-C chemokine receptor (CCR) 1, 3 and 5 expressed in tumor cells. However, the expression on CCL5 and its receptors have so far not been well-examined in human breast carcinoma tissues. We therefore immunolocalized CCL5, as well as CCR1, 3 and 5, in 111 human breast carcinoma tissues and correlated them with clinicopathological characteristics. Stromal CCL5 immunoreactivity was significantly correlated with the aggressive phenotype of breast carcinomas. Importantly, this tendency was observed especially in the CCR3-positive group. Furthermore, the risk of recurrence was significantly higher in the patients with breast carcinomas positive for CCL5 and CCR3 but negative for CCR1 and CCR5, as compared with other patients. In summary, the CCL5-CCR3 axis might contribute to a worse prognosis in breast cancer patients, and these findings will contribute to a better understanding of the significance of the CCL5/CCRs axis in breast carcinoma microenvironment.

  72. Suppression of tumor immune microenvironment via microRNA-1 after epidermal growth factor receptor-tyrosine kinase inhibitor resistance acquirement in lung adenocarcinoma. International-journal

    Sachiko Kawana, Ryoko Saito, Yasuhiro Miki, Yuichiro Kimura, Jiro Abe, Ikuro Sato, Mareyuki Endo, Shunichi Sugawara, Hironobu Sasano

    Cancer medicine　10　(2)　718-727　2021/01

    DOI： 10.1002/cam4.3639 　

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    Immunotherapy is considered one of the most important therapeutic strategies for patients with lung adenocarcinoma after the development of epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) resistance. However, useful predictors of immunotherapy for these patients has not been examined well, although the status of the tumor immune microenvironment (TIME), including programmed death-ligand 1 expression and lymphocyte infiltration, has been generally known to provide predictive markers for the efficacy of immunotherapy. This study aimed to clarify novel predictors of immunotherapy following EGFR-TKI resistance in lung adenocarcinoma, especially regarding micro RNA (miRNA). We evaluated the correlation between EGFR-TKI resistance and lymphocyte infiltration, before and after acquiring EGFR-TKI resistance, in 21 cases of lung adenocarcinoma, and further explored this by in vitro studies, using miRNA PCR arrays. Subsequently, we transfected miRNA-1 (miR-1), the most variable miRNA in this array, into three kinds of lung cancer cells, and examined the effects of miR-1 on EGFR-TKI sensitivity, cytokine expression and lymphocyte migration. Histopathological examination demonstrated that infiltration levels of CD8-positive T cells were significantly decreased after development of EGFR-TKI resistance. In vitro studies revealed that miR-1 significantly inhibited EGFR-TKI effect and induction of cytokines, such as C-C motif chemokine ligand 5 and C-X-C motif chemokine ligand 10, causing inhibition of monocyte migration. These results indicate that the upregulated miR-1 might suppress the TIME, following development of EGFR-TKI resistance. Therefore, miR-1 could be a clinically useful marker to predict therapeutic efficacy of immunotherapy in lung adenocarcinoma patients with EGFR-TKI resistance.

  73. 子宮内膜癌におけるMAP2の発現とDHEAとの関係

    三木 康宏, 岩渕 英里奈, 高木 清司, 鈴木 貴, 笹野 公伸, 伊藤 潔

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集　61回　50-50　2020/12

    Publisher: 日本組織細胞化学会

  74. Impact of COVID-19 restrictions on the research environment and motivation of researchers in Japan. International-journal

    Yasuhiro Miki, Natsuko Chubachi, Fumihiko Imamura, Nobuo Yaegashi, Kiyoshi Ito

    Progress in disaster science　8　100128-100128　2020/12

    DOI： 10.1016/j.pdisas.2020.100128 　

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    The environment surrounding researchers has changed significantly in the face of COVID-19 restrictions. An online self-reported questionnaire was completed by 10,557 participants between June 15 and 19, 2020. The impact on work/research activities and harassments under COVID-19 conditions was higher among researchers (1963) compared to non-researchers (8572). We further examined the effect of COVID-19 restrictions on 300 researchers. Women were significantly more likely to report being harassed than males. The overall "decrease in research motivation" was higher in women. The restrictions on research activities because of COVID-19 restrictions caused future anxiety and a decrease in research motivation.

  75. 乳癌の進展および治療耐性における活性型Rac1の意義

    高木 清司, 山口 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本乳癌学会総会プログラム抄録集　28回　219-219　2020/10

    Publisher: (一社)日本乳癌学会

  76. アンドロゲン作用を受けた腫瘍随伴マクロファージはCCL5-CCR3の相互作用を介し乳癌の進展を促進する

    山口 美桜, 高木 清司, 三木 康宏, 小野寺 好明, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本乳癌学会総会プログラム抄録集　28回　221-221　2020/10

    Publisher: (一社)日本乳癌学会

  77. インターロイキン17Aはトリプルネガティブ乳癌患者の予後不良因子である(Interleukin 17A is a poor prognostic factor for triple-negative breast cancer patients)

    Khalid Freeha, 高木 清司, 三木 康宏, 宮下 穣, 鈴木 貴, 笹野 公伸

    日本癌学会総会記事　79回　OE3-1　2020/10

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  78. 乳癌における活性型Rac1(Rac1-GTP)の発現意義

    高木 清司, 山口 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　79回　PJ14-3　2020/10

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  79. ヒト乳癌におけるD-2-ヒドロキシグルタレート脱水素酵素の発現意義

    林 千陽, 高木 清司, 佐藤 和, 峯村 洋行, 三木 康宏, 宮下 穣, 曽我 朋義, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　79回　PJ14-4　2020/10

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  80. アンドロゲン作用を受けた腫瘍随伴マクロファージはCCL5-CCR3相互作用を介して乳癌の進展に寄与する

    山口 美桜, 高木 清司, 三木 康宏, 小野寺 好明, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　79回　PJ14-5　2020/10

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  81. Interleukin 17A is a poor prognostic factor for triple-negative breast cancer patients(和訳中)

    Khalid Freeha, 高木 清司, 三木 康宏, 宮下 穣, 鈴木 貴, 笹野 公伸

    日本癌学会総会記事　79回　OE3-1　2020/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  82. 乳癌における活性型Rac1(Rac1-GTP)の発現意義

    高木 清司, 山口 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　79回　PJ14-3　2020/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  83. ヒト乳癌におけるD-2-ヒドロキシグルタレート脱水素酵素の発現意義

    林 千陽, 高木 清司, 佐藤 和, 峯村 洋行, 三木 康宏, 宮下 穣, 曽我 朋義, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　79回　PJ14-4　2020/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  84. アンドロゲン作用を受けた腫瘍随伴マクロファージはCCL5-CCR3相互作用を介して乳癌の進展に寄与する

    山口 美桜, 高木 清司, 三木 康宏, 小野寺 好明, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　79回　PJ14-5　2020/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  85. 乳癌の進展および治療耐性における活性型Rac1の意義

    高木 清司, 山口 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本乳癌学会総会プログラム抄録集　28回　219-219　2020/10

    Publisher: (一社)日本乳癌学会

  86. アンドロゲン作用を受けた腫瘍随伴マクロファージはCCL5-CCR3の相互作用を介し乳癌の進展を促進する

    山口 美桜, 高木 清司, 三木 康宏, 小野寺 好明, 宮下 穣, 笹野 公伸, 鈴木 貴

    日本乳癌学会総会プログラム抄録集　28回　221-221　2020/10

    Publisher: (一社)日本乳癌学会

  87. New Insights into Breast and Endometrial Cancers. International-journal

    Yasuhiro Miki

    Cancers　12　(9)　2020/09/11

    DOI： 10.3390/cancers12092595 　

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    Cancers of the breast and endometrium are some of the most common cancers affecting women [...].

  88. トリプルネガティブ乳癌におけるグルココルチコイドシグナルの意義に関する検討 アンドロゲンシグナルとの相互作用

    金井 綾子, May Mcnamara Keely, 岩渕 英里奈, 三木 康宏, 小野寺 好明, Fouzia Guestini, Freeha Khalid, 相良 安昭, 大井 恭代, 雷 哲明, 山口 倫, 田中 眞紀, 宮下 穣, 内藤 剛, 海野 倫明, 亀井 尚, 笹野 公伸, 石田 孝宣

    日本外科学会定期学術集会抄録集　120回　SF-1　2020/08

    Publisher: (一社)日本外科学会

  89. ホルモン依存性がん、副腎癌の新たな展開 ホルモン依存性癌におけるintracrineとホルモン受容体シグナルの可視化

    三木 康宏, 岩渕 英里奈, 鈴木 貴, 笹野 公伸, 伊藤 潔

    日本内分泌学会雑誌　96　(1)　119-119　2020/08

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  90. 11β hydroxysteroid dehydrogenase 1: a new marker for predicting response to immune-checkpoint blockade therapy in non-small-cell lung carcinoma. International-journal Peer-reviewed

    Ryoko Saito, Yasuhiro Miki, Takuto Abe, Eisaku Miyauchi, Jiro Abe, Ren Nanamiya, Chihiro Inoue, Ikuro Sato, Hironobu Sasano

    British journal of cancer　123　(1)　61-71　2020/04/27

    DOI： 10.1038/s41416-020-0837-3 　

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    BACKGROUND: Understanding the status of intratumoural immune microenvironment is necessary to ensure the efficacy of immune-checkpoint (IC) blockade therapy. Cortisol plays pivotal roles in glucocorticoid interactions in the immune system. We examined the correlation between intratumourally synthesised cortisol through 11β hydroxysteroid dehydrogenase (HSD) 1 and the immune microenvironment in non-small-cell lung carcinoma (NSCLC). METHODS: We correlated 11βHSD1 immunoreactivity in 125 cases of NSCLC with the amount of intratumoural immune cells present, and 11βHSD1 immunoreactivity with the efficacy of IC blockade therapy in 18 specimens of NSCLC patients. In vitro studies were performed to validate the immunohistochemical examination. RESULTS: 11βHSD1 immunoreactivity showed a significant inverse correlation with the number of tumour-infiltrating lymphocytes and CD3- or CD8-positive T cells. 11βHSD1 immunoreactivity tended to be inversely correlated with the clinical efficacy of the IC blockade therapy. In vitro studies revealed that 11βHSD1 promoted the intratumoural synthesis of cortisol. This resulted in a decrease in cytokines and in the inhibition of monocyte migration. CONCLUSIONS: Our study is the first report clarifying the inhibitory effects of intratumourally synthesised cortisol through 11βHSD1 on immune cell migration. We propose that the response to IC blockade therapy in NSCLC may be predicted by 11βHSD1.

  91. Rac1 activation in human breast carcinoma as a prognostic factor associated with therapeutic resistance Peer-reviewed

    Mio Yamaguchi, Kiyoshi Takagi, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Hironobu Sasano, Takashi Suzuki

    Breast Cancer　27　(5)　919-928　2020/04/20

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s12282-020-01091-2 　

    ISSN： 1340-6868

    eISSN： 1880-4233

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    BACKGROUND: RAS-related C3 botulinus toxin substrate 1 (Rac1) is a molecular switch fluctuating between GDP-bound inactive form (Rac1-GDP) and GTP-bound active form (Rac1-GTP) and involved in diverse function in both normal and malignant cells such as breast carcinoma cells. Although several studies have demonstrated immunolocalization of Rac1 protein in human breast carcinoma tissues, activation status of Rac1 still remains to be elucidated. METHODS: We immunolocalized active form of Rac1 (Rac1-GTP) as well as total Rac1 using antibody specific for them in 115 invasive breast carcinoma tissues and correlated with clinicopathological parameters and clinical outcomes. RESULTS: Rac1-GTP was frequently immunolocalized in the cytoplasm or cell membrane of breast carcinoma cells and it was positively correlated with Ki-67 labeling index and total Rac1 while negatively correlated with progesterone receptor. On the other hand, immunohistochemical Rac1-GTP status was significantly correlated with increased risk of recurrence and breast cancer-specific mortality of breast cancer patients and multivariate analyses did demonstrate Rac1-GTP as an independent worse prognostic factor for both disease-free and breast cancer-specific survival. In addition, Rac1-GTP was still correlated with worse prognosis in the patients who had received adjuvant chemotherapy or endocrine therapy. CONCLUSION: These findings suggested Rac1 activation played pivotal roles in the progression and therapeutic resistance of breast cancers and Rac1 might be an important therapeutic target for improvement of the therapy for breast cancer patients.

  92. Vasohibin-1 and miR-720 expression in diffuse pulmonary capillary hemangiomatosis-like changes associated with pulmonary hypoplasia. International-journal Peer-reviewed

    Ryoko Saito, Atsuko Kasajima, Yoshinori Kawabata, Yasuhiro Miki, Junya Tominaga, Shunsuke Tatebe, Hiromichi Nakajima, Shuko Hata, Chihiro Inoue, Shinji Taniuchi, Fumiyoshi Fujishima, Mika Watanabe, Yasufumi Sato, Yoshinori Okada, Hironobu Sasano

    Pathology international　70　(7)　470-472　2020/04/20

    DOI： 10.1111/pin.12931 　

  93. ヒト非小細胞肺癌細胞におけるPD-L1発現予測因子としての病理学的核形態の探索

    齊藤 涼子, 三木 康宏, 山崎 有人, 阿部 二郎, 井上 千裕, 佐藤 郁郎, 笹野 公伸

    日本病理学会会誌　109　(1)　410-410　2020/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  94. 【子宮頸がん予防-日本はどうする?-】日本の子宮頸がん検診の現状と課題

    伊藤 潔, 三木 康宏, 小澤 信義, 八重樫 伸生

    産婦人科の実際　69　(3)　213-217　2020/03

    Publisher: 金原出版(株)

    ISSN： 0558-4728

  95. 子宮内膜癌におけるKrueppel-like factor 5(KLF5)の発現 Peer-reviewed

    三木 康宏, 高木 清司, 鈴木 貴, 伊藤 潔

    日本内分泌学会雑誌　95　(4)　1550-1550　2020/02

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  96. Significance of glucocorticoid signaling in triple-negative breast cancer patients: a newly revealed interaction with androgen signaling. International-journal Peer-reviewed

    Ayako Kanai, Keely May McNamara, Erina Iwabuchi, Yasuhiro Miki, Yoshiaki Onodera, Fouzia Guestini, Freeha Khalid, Yasuaki Sagara, Yasuyo Ohi, Yoshiaki Rai, Rin Yamaguchi, Maki Tanaka, Minoru Miyashita, Takanori Ishida, Hironobu Sasano

    Breast cancer research and treatment　180　(1)　97-110　2020/02

    DOI： 10.1007/s10549-020-05523-7 　

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    PURPOSE: Chemotherapy is the only current effective systemic treatment for triple-negative breast cancer (TNBC) patients. Therefore, the identification of active biological pathways that could become therapeutic targets is crucial. In this study, considering the well-reported biological roles of glucocorticoid and androgen receptors (GR, AR) in TNBC, we attempted to explore the effects of glucocorticoids (GCs) on cell kinetics as well as the potential interaction between GR and AR in TNBC. METHODS: We first explored the association between the status of GR, AR, and/or GCs-metabolizing enzymes such as 11β-hydroxysteroid dehydrogenase (11βHSD) 1 and 2 and the clinicopathological variables of the TNBC patients. Thereafter, we also studied the effects of dexamethasone (DEX) with/without dihydrotestosterone (DHT) on TNBC cell lines by assessing the cell proliferation, migration and GC response genes at the transcriptional level. RESULTS: GR positivity in carcinoma cells was significantly associated with adverse clinical outcome of the patients and AR positivity was significantly associated with lower histological grade and Ki-67 labeling index of the cases examined. In particular, AR positivity was significantly associated with decreased risks of developing recurrence in GR-positive TNBC patients. The subsequent in vitro studies revealed that DEX-promoted cell migration was inhibited by the co-treatment with DHT in GR/AR double-positive HCC38 cells. In addition, DHT inhibited the DEX-increased serum and glucocorticoid-regulated kinase-1 (SGK1) mRNA expression. CONCLUSION: This is the first study to reveal that the interaction of GR and AR did influence the clinical outcome of TNBC patients and GCs induced cell migration in TNBC cells.

  97. Systemic distribution of progesterone receptor subtypes in human tissues. International-journal Peer-reviewed

    Teeranut Asavasupreechar, Ryoko Saito, Yasuhiro Miki, Dean P Edwards, Viroj Boonyaratanakornkit, Hironobu Sasano

    The Journal of steroid biochemistry and molecular biology　199　105599-105599　2020/01/25

    DOI： 10.1016/j.jsbmb.2020.105599 　

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    Progesterone receptor (PR) is expressed in a wide variety of human tissues, including both reproductive and non-reproductive tissues. Upon binding to the PR, progesterone can display several non-reproductive functions, including neurosteroid activity in the central nervous system, inhibition of smooth muscle contractile activity in the gastrointestinal tract, and regulating the development and maturation of the lung. PR exists as two major isoforms, PRA and PRB. Differential expression of these PR isoforms reportedly contributes to different biological activities of the hormone. However, the distribution of the PR isoforms in human tissues has remained virtually unexplored. In this study, we immunolocalized PR expression in various human tissues using PR (1294) specific antibody, which is capable of detecting both PRA and PRB, and PRB (250H11) specific antibody. Tissues from the uterus, ovary, breast, placenta, prostate, testis, cerebrum, cerebellum, pituitary, spinal cord, esophagus, stomach, small intestine, colon, pancreas, liver, kidney, urinary bladder, lung, heart, aorta, thymus, adrenal gland, thyroid, spleen, skin, and bone were examined in four different age groups (fetal, pediatric, young, and old) in male and female subjects. PR and PRB were detected in the nuclei of cells in the female reproductive system, in both the nuclei and cytoplasm of pituitary gland and pancreatic acinar cells, and only in the cytoplasm of cells in the testis, stomach, small intestine, colon, liver, kidney, urinary bladder, lung, adrenal gland, and skin. Of particular interest, total PRB expression overlapped with that of total PR expression in most tissues but was negative in the female fetal reproductive system. The findings indicate that progesterone could affect diverse human organs differently than from reproductive organs. These findings provide new insights into the novel biological roles of progesterone in non-reproductive organs.

  98. Immunohistochemical assessment of growth factor signaling molecules: MAPK, Akt, and STAT3 pathways in oral epithelial precursor lesions and squamous cell carcinoma

    Kazuki Tashiro, Mariko Oikawa, Yasuhiro Miki, Tetsu Takahashi, Hiroyuki Kumamoto

    Odontology　108　(1)　91-101　2020/01/01

    DOI： 10.1007/s10266-019-00428-4 　

    ISSN： 1618-1247

    eISSN： 1618-1255

  99. Immunohistochemical assessment of growth factor signaling molecules: MAPK, Akt, and STAT3 pathways in oral epithelial precursor lesions and squamous cell carcinoma.

    Kazuki Tashiro, Mariko Oikawa, Yasuhiro Miki, Tetsu Takahashi, Hiroyuki Kumamoto

    Odontology　108　(1)　91-101　2020/01

    DOI： 10.1007/s10266-019-00428-4 　

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    Several growth factors and their receptors, such as epidermal growth factor receptor, have been studied as prognostic biomarkers for many epithelial malignancies. The signal transduction cascade of those receptors includes RAS/RAF/ERK, PI3K/Akt/mTOR, and STAT3 pathways. The aim of this study was to investigate the expression levels of several key proteins of those pathways in patients with oral squamous cell carcinoma (OSCC) and oral epithelial precursor lesions (OEPLs), and to correlate the expressions of these proteins with clinicopathologic features and prognosis. Fifteen leukoplakia (LP), 15 low-grade epithelial dysplasia, 15 high-grade epithelial dysplasia (HD), and 132 OSCC specimens were immunohistochemically examined for KRAS, HRAS, NRAS, BRAF, pERK1/2, pAkt, pmTOR, and pSTAT3 expression. Immunoreactivity for these molecules predominantly occurred in regions OEPL basal to prickle layers and in most OSCC cells. KRAS and NRAS expression was significantly lower in OSCC than in OEPLs, while pAkt and pmTOR showed higher expression in OSCC than in OEPLs. pERK1/2 expression was significantly higher in HD than in LP. In OSCC, KRAS and NRAS immunoreactivity was significantly higher in advanced age and male gender. In addition, higher immunoreactivity was shown in pERK1/2 in female gender and advanced TNM stage, pAkt in advanced T classification and cases without postoperative metastasis, pmTOR in advanced mode of invasion, and pSTAT3 in invasion depth. Correlations between these markers and clinicopathological variables were also noted. MAPK, Akt, and STAT3 pathways might play diverse roles in oral carcinogenesis.

  100. Cervical cancer screening rates before and after the Great East Japan Earthquake in the Miyagi Prefecture, Japan. International-journal

    Yasuhiro Miki, Toru Tase, Hideki Tokunaga, Nobuo Yaegashi, Kiyoshi Ito

    PloS one　15　(3)　e0229924　2020

    DOI： 10.1371/journal.pone.0229924 　

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    After disasters, issues pertaining to women's health such as irregular periods and bleeding are well surveyed. However, the management of women's health, especially changes in the rate of health checkups, has not been investigated. In the present study, we focused on the change in the cervical cancer screening rates (CCS-Rs) before and after the Great East Japan Earthquake in Miyagi Prefecture, Japan. The earthquake had a magnitude of 9.0, a profound disaster. We examined the CCS-R from 2009 to 2016 in 45 areas of the Miyagi Prefecture. Screening was completed using mobile vans. In the 4 areas impacted by the tsunami after the earthquake, a marked decrease in the CCS-R was observed in 2011 when the earthquake took place (more than a 3% decrease compared with that in the previous year). The CCS-Rs in these 4 regions remained lower in 2016 than in the previous year. In 2009-2016 except for 2014, CCS-Rs in coastal areas (9 areas) were significantly lower than those in the non-coastal areas (36 areas). A delay in seeking healthcare, also known as "patient's delay," is considered as one of the problems of cancer treatment in affected areas. It is possible that a decrease in the CCS-R may lead to low detection of advanced stages of cancer. Therefore, the establishment of a comprehensive medical system including medical screening after a disaster is important for the management of women's health.

  101. Sex steroid metabolism and actions in non-small cell lung carcinoma. International-journal Peer-reviewed

    Asavasupreechar T, Chan MSM, Saito R, Miki Y, Boonyaratanakornkit V, Sasano H

    The Journal of steroid biochemistry and molecular biology　193　105440-105440　2019/10

    DOI： 10.1016/j.jsbmb.2019.105440 　

    ISSN： 0960-0760

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    Despite recent development in targeted therapies, lung cancer still remains the leading cause of cancer death. Therefore, a better understanding of its pathogenesis and progression could contribute to improving the eventual clinical outcome of the patients. Results of recently published several in vitro and clinical studies indicated the possible involvement of sex steroids in both development and progression of non-small cell lung carcinoma (NSCLC). Therefore we summarized the reported clinical relevant information of the sex steroids, their receptors and steroid metabolizing enzymes related to NSCLC in this mini-review. In addition, we also reviewed the potential "endocrine therapy", targeting sex steroid actions and/or metabolism in NSCLC patients.

  102. 腫瘍随伴マクロファージにおけるアンドロゲン誘導性液性因子CCL5は乳癌の進展に寄与する(Androgen-induced C-C motif chemokine ligand 5 secretion of macrophages regulate breast cancer progression) Peer-reviewed

    山口 美桜, 高木 清司, 三木 康宏, 小野寺 好明, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　78回　P-1340　2019/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  103. CLEC2Dの乳癌における発現意義(Significance of CLEC2D expression in breast cancer) Peer-reviewed

    栗原 唯, 高木 清司, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　78回　P-1341　2019/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  104. エストロゲン受容体陽性浸潤性乳癌におけるCYC1の発現意義(Cytochrome c1 in estrogen receptor-positive breast carcinoma) Peer-reviewed

    佐藤 和, 高木 清司, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　78回　P-1342　2019/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  105. PD-L1 Induction by Cancer-Associated Fibroblast-Derived Factors in Lung Adenocarcinoma Cells. Peer-reviewed

    Inoue C, Miki Y, Saito R, Hata S, Abe J, Sato I, Okada Y, Sasano H

    Cancers　11　(9)　1257-1257　2019/08

    Publisher: MDPI AG

    DOI： 10.3390/cancers11091257 　

    eISSN： 2072-6694

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    Cancer-associated fibroblasts (CAFs) exert various effects upon biological behaviours of cancer. In this study, we examined the correlation of CAFs with the intra-tumoural immune system in the lung adenocarcinoma microenvironment. We studied 27 and 113 cases of lung adenocarcinoma tentatively as Cohorts 1 and 2, respectively. The patients in Cohort 1 received epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) for recurrent lung adenocarcinoma. -smooth muscle actin (-SMA), a surrogate marker for CAFs, was examined by immunohistochemistry. We then examined the effects of CAFs isolated from lung cancer tissues on programmed death ligand 1 (PD-L1) expression in lung adenocarcinoma cell lines. No significant associations were detected between -SMA status and the ratios of CD8/CD4 and Foxp3/CD8 in Cohort 1. However, -SMA status was significantly associated with PD-L1 status in both Cohorts 1 and 2. Conditioned medium of CAFs significantly induced PD-L1 expression in lung adenocarcinoma cell lines, A549, PC-9, and H1975. Among the cytokines examined by antibody array, C-X-C motif chemokine ligand 2 (CXCL2) increased PD-L1 mRNA expression in these cell lines. CXCL2 is therefore considered to have a potential to induce PD-L1 expression in lung adenocarcinoma cells as a result of an interaction between carcinoma cells and CAFs. These findings did firstly demonstrate that CAFs indirectly influenced tumour immunity through increasing PD-L1 expression in lung adenocarcinoma cells.

  106. Prognostic significance of combining immunohistochemical markers for cancer-associated fibroblasts in lung adenocarcinoma tissue. International-journal Peer-reviewed

    Inoue C, Tamatsuki D, Miki Y, Saito R, Okada Y, Sasano H

    Virchows Archiv : an international journal of pathology　475　(2)　181-189　2019/08

    DOI： 10.1007/s00428-019-02587-9 　

    ISSN： 0945-6317

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    Cancer-associated fibroblasts (CAFs), activated fibroblasts in a cancer microenvironment, exert various effects upon carcinoma cells including lung adenocarcinoma cells. Various markers identifying CAFs have been proposed, but the correlations among these markers proposed and their clinicopathological significance have remained largely unknown. Therefore, in this study, we immunohistochemically evaluated the expression of alpha-smooth muscle actin (α-SMA), podoplanin, and periostin among these proposed markers in 92 cases of lung adenocarcinoma. These three markers were weakly correlated, but the relative abundance of α-SMA was significantly associated with high Ki-67 labelling index (LI), lymph node metastasis, and low 5-year overall survival (OS) rate of the patients. That of podoplanin was significantly associated with high pT and Ki-67 LI, distant metastasis, and low 5-year OS rate and that of periostin with high pT and Ki-67 LI. We then tentatively subclassified these cases into four groups according to high or low status of each of paired markers: α-SMA/podoplanin, α-SMA/periostin, and periostin/podoplanin. The α-SMA high/podoplanin high group was associated with the lowest survival rate (53.3%) among the four groups with significance. However, there were no significant differences in overall survival when the patients were classified according to the combinations of α-SMA/periostin or periostin/podoplanin. Results of our study firstly revealed the heterogeneity of CAFs in human lung adenocarcinoma tissue, and the analysis employing multiple markers of CAFs is generally required to study the clinical significance of CAFs in clinical materials.

  107. Amyloid precursor protein and its phosphorylated form in non-small cell lung carcinoma. International-journal Peer-reviewed

    Ito S, Miki Y, Saito R, Inoue C, Okada Y, Sasano H

    Pathology, research and practice　215　(8)　152463-152463　2019/08

    DOI： 10.1016/j.prp.2019.152463 　

    ISSN： 0344-0338

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    Amyloid precursor protein (APP) is a well-known to be involved in the development of Alzheimer's disease and harbors several phosphorylation sites in its cytoplasmic domain. APP has been also proposed as one of the molecules involved in cell proliferation and invasion in several human malignancies. However, the roles of APP including its phosphorylated form (p-APP) have remained largely unexplored in non-small cell lung carcinoma (NSCLC). Therefore, in this study, we first examined both APP and p-APP expressions and then explored the association between p-APP/APP status and clicopathological parameters in NSCLC. The number of APP-positive cases was 24/91 (26%) in adenocarcinomas (Ad) and 16/35 (46%) in squamous cell carcinomas (Sq), respectively. p-APP-positive cases in Ad and Sq were 28 (31%) and 17 (49%), respectively. In Ad cases, both APP and p-APP were significantly associated with clinical stages (APP and p-APP), pathologic T (p-APP), and pathologic N (APP and p-APP) of the cases examined. In Sq cases, there were no significant associations between APP status and any of the clinicopathological parameters examined with an exception of the significant correlation of p-APP with lymphatic invasion. APP status was not significantly associated with overall survival (OS) of Ad patients but a significant association was detected between p-APP-positive cases and OS of these patients (p < 0.0001). In Sq cases, both APP- (p = 0.01) and p-APP-positive (p = 0.04) groups were also significantly associated with adverse clinical outcome. These results did firstly demonstrate that APP, in particular, p-APP, is considered a potent prognostic factor for both Ad and Sq lung carcinoma patients. However, APP signaling including its phosphorylation signal are considered different between these two types of NSCC cells and further investigations are required for clarification.

  108. The significance of lipid accumulation in breast carcinoma cells through perilipin 2 and its clinicopathological significance. International-journal Peer-reviewed

    Kuniyoshi S, Miki Y, Sasaki A, Iwabuchi E, Ono K, Onodera Y, Hirakawa H, Ishida T, Yoshimi N, Sasano H

    Pathology international　69　(8)　463-471　2019/07

    DOI： 10.1111/pin.12831 　

    ISSN： 1320-5463

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    Both systemic and intratumoral lipid metabolism have been recently reported to play pivotal roles in both tumor development and progression in various human malignancies including breast cancer. However, its details have remained largely unknown in breast cancer patients. Therefore, in this study, we focused on perilipin 2, which is involved in constituting the intracellular lipid composition. Perilipin 2 was first immunolocalized in 105 cases of breast cancer. The status of perilipin 2 immunoreactivity was significantly positively associated with histological grade, Ki-67 labeling index and HER2 status and negatively with estrogen receptor status of these patients. Subsequent in vitro study also revealed that its mRNA expression in triple negative breast carcinoma cells was higher than cells of other subtypes. We then examined the correlation between perilipin 2 immunoreactivity and intracellular lipid droplet evaluated by Oil-red O stating in 13 cases of breast carcinoma tissues. A significantly positive correlation was detected between the status of perilipin 2 and Oil-red O staining. These findings above did indicate that perilipin 2 could represent the status of intracellular lipid droplets in surgical pathology specimens of breast cancer and perilipin 2 was also associated with its more aggressive biological phenotypes.

  109. 乳癌の進行におけるCLEC2Dの役割に関する検討 Peer-reviewed

    栗原 唯, 高木 清司, 保田 伊織, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    宮城県臨床検査技師会誌　9　(1)　32-32　2019/06

    Publisher: (一社)宮城県臨床検査技師会

    ISSN： 2186-6899

  110. 免疫組織化学法による乳癌組織随伴マクロファージにおけるアンドロゲン誘導性液性因子CCL5の発現意義の検討 Peer-reviewed

    山口 美桜, 高木 清司, 佐藤 正康, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    宮城県臨床検査技師会誌　9　(1)　33-33　2019/06

    Publisher: (一社)宮城県臨床検査技師会

    ISSN： 2186-6899

  111. Co-expression of carcinoembryonic antigen-related cell adhesion molecule 6 and 8 inhibits proliferation and invasiveness of breast carcinoma cells. International-journal Peer-reviewed

    Iwabuchi E, Miki Y, Onodera Y, Shibahara Y, Takagi K, Suzuki T, Ishida T, Sasano H

    Clinical & experimental metastasis　36　(5)　423-432　2019/06

    DOI： 10.1007/s10585-019-09981-2 　

    ISSN： 0262-0898

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    The carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 6 and CEACAM8 form heterodimers and exert their effects. Therefore, we examined the effects of CEACAM6 and CEACAM8 co-expression in breast cancer. We first studied CEACAM6/8 expression using immunohistochemistry in 109 patients with breast cancer. We then established MCF-7 cells that were stably transfected with CEACAM8 and studied cell proliferation, invasion and adhesion. The number of CEACAM6 and CEACAM8 double-positive breast carcinoma cells significantly increased in patients with low histopathological grade and stage. Proximity ligation assay (PLA) confirmed high CEACAM6/8 expression in MCF-7 cells. CEACAM6/8 expression promoted the adhesion of MCF-7 cells to endothelial cell monolayers but inhibited their invasion and proliferation. Furthermore, CEACAM6 status in carcinoma cells was significantly higher in bone than in lung metastases. CEACAM6/8 expression is associated with the inhibition of vascular invasion and cell proliferation. CEACAM6 expression was also considered to be involved in bone metastases of breast cancer. This is the first study to demonstrate the possible role of CEACAM6/8 heterodimer and CEACAM6 expression in breast cancer patients.

  112. Multiple primary cancers associated with endometrial and ovarian cancers: An analysis based upon the Japan Autopsy Annual Database from 2002 to 2010. International-journal Peer-reviewed

    Miki Y, Sugawara Y, Shibahara Y, Tsuji I, Sasano H, Ito K

    The journal of obstetrics and gynaecology research　45　(5)　1012-1018　2019/05

    DOI： 10.1111/jog.13934 　

    ISSN： 1341-8076

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    AIM: A thorough analysis of multiple primary cancers (MPC) could provide important information as to the pathogenesis of human malignancies. Analysis of MPC using clinical databases has been performed, but little has been done using autopsy cases. Therefore, in this study, we first retrospectively analyzed MPC associated with endometrial and ovarian cancers using the Japan Autopsy Annual Database. METHODS: The Japan Autopsy Annual Database from 2002 to 2010 was established by the Japanese Society of Pathology, Tokyo, Japan. Among the 164 211 autopsy cases registered, 9142 were cases of primary cancers. RESULTS: The patients with endometrial cancer-associated MPC did have a lower risk of harboring colorectal cancer (odds ratio [OR], 0.63; 95% confidence interval [CI], 0.45-0.89) but had a higher risk of ovarian cancer (OR, 3.15; 95% CI, 2.11-4.71). Those with ovarian cancer-associated MPC had a lower risk of harboring gallbladder cancer including bile ductal cancer (OR, 0.47; 95% CI, 0.27-0.80) but a higher risk of harboring breast (OR, 1.69; 95% CI, 1.20-2.38) and endometrial cancers (OR, 3.48; 95% CI, 2.33-5.20). CONCLUSION: Both endogenous and exogenous factors are associated with the incidence of MPC. Results of our present study based on Japanese Autopsy Base first demonstrated that female hormones had a strong influence on the incidence of MPC. This study also demonstrated that the analysis of MPC using an autopsy database could have advantages over clinical database analysis.

  113. Cytochrome c1 as a favorable prognostic marker in estrogen receptor-positive breast carcinoma. International-journal Peer-reviewed

    Sato A, Takagi K, Miki Y, Yoshimura A, Hara M, Ishida T, Sasano H, Suzuki T

    Histology and histopathology　34　(12)　18130-1375　2019/05

    DOI： 10.14670/HH-18-130 　

    ISSN： 0213-3911

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    BACKGROUND: Cytochrome c1 (CYC1) is a heme-containing subunit of mitochondria complex III and is mainly involved in cellular energy production. A recent study has demonstrated that CYC1 was overexpressed in breast carcinoma tissues and induced proliferation, migration and invasion of estrogen receptor (ER)-negative breast carcinoma cells. However, the clinical significance of CYC1 protein remains largely unclear in invasive breast carcinoma, and biological functions of CYC1 have not been reported in ER-positive breast carcinoma cells. MATERIALS AND METHODS: We immunolocalized CYC1 in 172 invasive breast carcinomas and evaluated its clinical significance according to the ER-status. Subsequently, we examined the effects of CYC1 on proliferation, glycolysis and chemosensitivity to paclitaxel, which is one of the most common chemotherapeutic agents in breast cancer, in ER-positive breast carcinoma cells (MCF7 and T47D). RESULTS: CYC1 immunoreactivity was detected in 47% of ER-positive cases and 30% of ER-negative cases. Immunohistochemical CYC1 status was inversely associated with Ki67 in ER-positive cases, and it was a significantly favorable prognostic factor for both disease-free and breast cancer-specific survival of the patients. On the other hand, no significant association was detected between CYC1 status and clinicopathological factors in ER-negative cases. In in vitro experiments, MCF7 and T47D cells transfected specific siRNA for CYC1 significantly increased cell proliferation activity, L-lactate production and cell viability after paclitaxel treatment. CONCLUSION: These results suggest that CYC1 inhibits cell proliferation, glycolytic activity and increases chemosensitivity to paclitaxel in ER-positive breast carcinoma cells and that CYC1 status is a potent favorable prognostic factor in ER-positive breast cancer patients.

  114. Lymphangioleiomyomatosis(LAM)におけるVasohibin-1,vasohibin-2の発現と意義

    井上 千裕, 三木 康宏, 齊藤 涼子, 小林 数真, 岡田 克典, 笹野 公伸

    日本病理学会会誌　108　(1)　472-472　2019/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  115. 子宮内膜癌におけるステロイドホルモンとKrueppel-like factor 5の発現

    三木 康宏, 吉田 伶奈, 高木 清司, 鈴木 貴, 伊藤 潔

    日本内分泌学会雑誌　95　(1)　445-445　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  116. ホルモン依存性癌の新展開 ホルモン依存性癌におけるホルモンシグナルの可視化 Peer-reviewed

    三木 康宏, 岩渕 英里奈, 鈴木 貴, 笹野 公伸, 伊藤 潔

    日本内分泌学会雑誌　95　(1)　196-196　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  117. 乳癌におけるKLK12の発現意義 Peer-reviewed

    吉村 彩乃, 塚本 若菜, 佐藤 和, 高木 清司, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　445-445　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  118. ER陽性浸潤性乳癌におけるCYC1の発現意義 Peer-reviewed

    佐藤 和, 高木 清司, 三木 康宏, 吉村 彩乃, 原 瑞季, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　473-473　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  119. 乳癌における5α-reductase type 3の発現意義 Peer-reviewed

    高木 清司, 佐藤 和, 井上 直紀, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　473-473　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  120. 乳癌におけるCLEC2Dの発現意義 Peer-reviewed

    栗原 唯, 高木 清司, 保田 伊織, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　488-488　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  121. 乳癌組織随伴マクロファージにおけるアンドロゲン誘導性液性因子の解析 Peer-reviewed

    山口 美桜, 高木 清司, 佐藤 正康, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　488-488　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  122. The correlation of p22phox and chemosensitivity in EGFR-TKI resistant lung adenocarcinoma. International-journal Peer-reviewed

    Kobayashi M, Saito R, Miki Y, Nanamiya R, Inoue C, Abe J, Sato I, Okada Y, Sasano H

    Oncotarget　10　(10)　1119-1131　2019/02

    DOI： 10.18632/oncotarget.26637 　

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    Background: Enhancing the chemosensitivity in the patients with epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) resistant lung adenocarcinoma (LUAD) is pivotal in achieving their successful therapeutic outcome. We aimed to explore the mechanisms regarding the development of therapeutic resistance to chemotherapy in EGFR-TKI resistant LUAD. Methods: Microarray analysis lead to potential involvement of p22phox, which was abundantly expressed in the cell lines harboring EGFR-TKI resistance and chemoresistance, and was known to regulate several important chemoresistance-associated factors such as hypoxia inducible factor-1α (HIF-1α) and epithelial-mesenchymal transition (EMT). We compared the status of p22phox with that of chemoresistance, HIF-1α expression and EMT in LUAD cell lines. We immunolocalized p22phox in the specimens of lung cancer patients. Results: p22phox and HIF-1α mRNAs were significantly elevated in the cells harboring EMT and chemoresistance. p22phox knockdown enhanced chemosensitivity and reduced the expression of HIF-1α and EMT-associated factors. HIF-1α knockdown enhanced the chemosensitivity, while HIF-1α transfection induced EMT and chemoresistance in these cell lines. All LUAD patients with T790M mutation were associated with abundant p22phox immunoreactivity in carcinoma cells. Conclusions: The analysis of p22phox in lung carcinoma tissues could provide new insights into the selection of chemotherapy for the patients with EGFR-TKI resistant LUAD.

  123. The interaction between carcinoembryonic antigen-related cell adhesion molecule 6 and human epidermal growth factor receptor 2 is associated with therapeutic efficacy of trastuzumab in breast cancer. International-journal Peer-reviewed

    Erina Iwabuchi, Yasuhiro Miki, Ayako Kanai, Minoru Miyashita, George Kijima, Hisashi Hirakawa, Takashi Suzuki, Takanori Ishida, Hironobu Sasano

    The Journal of pathology　246　(3)　379-389　2018/11

    DOI： 10.1002/path.5148 　

    ISSN： 0022-3417

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    Human epidermal growth factor receptor 2 (HER2) is a target of the HER2 inhibitor trastuzumab, which has been administered to HER2-positive breast cancer patients. However, the therapeutic effects of HER2 inhibitor monotherapy are not always clinically effective as compared with cotreatment with chemotherapy. Therefore, it has become pivotal to predict the therapeutic efficacy of trastuzumab monotherapy prior to administration. Recently, carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) has been reported to be a HER2-related factor. The aim of the present study was to explore the therapeutic mechanism of trastuzumab, including the relevance of CEACAM6 expression. CEACAM6/HER2-double-positive human breast carcinoma cell lines BT-474, HCC-1419 and MDA-MB-361 were used in this study. CEACAM6 knockdown decreased the inhibitory effects of trastuzumab in the trastuzumab-sensitive BT-474 and HCC-1419 cells, but not in the trastuzumab-resistant MDA-MB-361 cells. We examined the interaction between CEACAM6 and HER2 by using a proximity ligation assay (PLA). The interaction was detected in BT-474 and HCC-1419 cells, but not in MDA-MB-361 cells, and was significantly associated with in vitro trastuzumab therapeutic sensitivity. We further analysed the status of CEACAM6 and HER2 and their interaction in archival pathology specimens, also using PLA. The interaction was detected only in CEACAM6/HER2-double-positive breast cancer cases, and their PLA score was significantly associated with the efficacy of trastuzumab treatment. Therefore, evaluation of the CEACAM6-HER2 interaction could serve as a marker to predict the efficacy of trastuzumab monotherapy in breast cancer patients. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

  124. Exploring Protein⁻Protein Interaction in the Study of Hormone-Dependent Cancers. Peer-reviewed

    Miki Y, Iwabuchi E, Ono K, Sasano H, Ito K

    International journal of molecular sciences　19　(10)　2018/10

    DOI： 10.3390/ijms19103173 　

  125. OLFM4, LY6D and S100A7 as potent markers for distant metastasis in estrogen receptor-positive breast carcinoma. International-journal Peer-reviewed

    Akifumi Mayama, Kiyoshi Takagi, Hiroyoshi Suzuki, Ai Sato, Yoshiaki Onodera, Yasuhiro Miki, Minako Sakurai, Takanori Watanabe, Kazuhiro Sakamoto, Ryuichi Yoshida, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    Cancer science　109　(10)　3350-3359　2018/10

    DOI： 10.1111/cas.13770 　

    ISSN： 1347-9032

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    Metastatic breast cancer is a highly lethal disease, and it is very important to evaluate the biomarkers associated with distant metastasis. However, molecular features of distant metastasis remain largely unknown in breast cancer. Estrogens play an important role in the progression of breast cancer and the majority of stage IV breast carcinomas express estrogen receptor (ER). Therefore, in this study, we examined molecular markers associated with distant metastasis in ER-positive breast carcinoma by microarray and immunohistochemistry. When we examined the gene expression profile of ER-positive stage IV breast carcinoma tissues (n = 7) comparing ER-positive stage I-III cases (n = 11) by microarray analysis, we newly identified OLFM4, LY6D and S100A7, which were closely associated with the distant metastasis. Subsequently, we performed immunohistochemistry for OLFM4, LY6D and S100A7 in 168 ER-positive breast carcinomas. OLFM4, LY6D and S100A7 immunoreactivities were significantly associated with stage, pathological T factor, distant metastasis and Ki67 status in the ER-positive breast carcinomas. Moreover, these immunoreactivities were significantly associated with a worse prognostic factor for distant metastasis-free and breast cancer-specific survival in ER-positive stage I-III breast cancer patients. However, when we performed immunohistochemistry for OLFM4, LY6D and S100A7 in 40 ER-negative breast carcinomas, these immunoreactivities were not generally associated with the clinicopathological factors examined, including distant metastasis and prognosis of patients, in this study. These results suggest that OLFM4, LY6D and S100A7 immunoreactivity are associated with an aggressive phenotype of ER-positive breast carcinoma, and these are potent markers for distant metastasis of ER-positive breast cancer patients.

  126. 乳癌におけるCytokeratin 19の発現局在の検討 Peer-reviewed

    岩渕 英里奈, 三木 康宏, 金井 綾子, 高木 清司, 鈴木 貴, 石田 孝宣, 笹野 公伸

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集　59回　79-79　2018/09

    Publisher: 日本組織細胞化学会

  127. S100P and Ezrin promote trans-endothelial migration of triple negative breast cancer cells. Peer-reviewed

    Kikuchi K, McNamara KM, Miki Y, Iwabuchi E, Kanai A, Miyashita M, Ishida T, Sasano H

    Cellular oncology (Dordrecht)　42　(1)　67-80　2018/09

    DOI： 10.1007/s13402-018-0408-2 　

    ISSN： 2211-3428

    eISSN： 2211-3436

  128. Relaxin 2/RXFP1 Signaling Induces Cell Invasion via the β-Catenin Pathway in Endometrial Cancer. International-journal Peer-reviewed

    Misaki Fue, Yasuhiro Miki, Kiyoshi Takagi, Chiaki Hashimoto, Nobuo Yaegashi, Takashi Suzuki, Kiyoshi Ito

    International journal of molecular sciences　19　(8)　2018/08/18

    DOI： 10.3390/ijms19082438 　

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    Relaxin is known to play an important role in animal pregnancies, including those of humans. It is suggested that relaxin induces aggressive cell growth and invasiveness in several types of cancer, including endometrial cancer. However, the mechanisms of relaxin remain largely unclear. In this study, we examined the effects of relaxin 2 (RLN2), the major circulating relaxin in humans, on human endometrial carcinoma cell lines. RLN2 treatment induced invasion in HEC-1B and Ishikawa cells. RLN2-induced cell invasion was significantly decreased by transfection of relaxin receptor 1 (RXFP1) siRNAs. The β-catenin inhibitor, XAV939, also significantly inhibited the RLN2-induced cell invasions. Both a decrease of cadherin expression and an increase of β-catenin phosphorylation were observed in response to the RLN2 treatment in HEC-1B and Ishikawa cells. We then examined RLN2 and RXFP1 expression in 80 human endometrioid endometrial carcinoma tissues. RLN2 immunoreactivity was detected in the human endometrial carcinoma cells and had a correlative tendency with histological grade and RXFP1. These results suggest that adherens junctions in cancer cells are weakened by the breakdown of the cadherin/catenin complex, which is induced by β-catenin phosphorylation via RLN2/RXFP1 signaling.

  129. 口腔癌および前癌病変における増殖因子シグナル伝達分子の発現とBRAF-V600E遺伝子変異に関する臨床病理学的研究

    田代 和樹, 及川 麻理子, 佐藤 郁郎, 春日井 勲, 三木 康宏, 清水 良央, 高橋 哲, 熊本 裕行

    日本口腔科学会雑誌　67　(2)　184-185　2018/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

  130. Tumor microenvironment in functional adrenocortical adenomas: immune cell infiltration in cortisol-producing adrenocortical adenoma. International-journal Peer-reviewed

    Yuko Kitawaki, Yasuhiro Nakamura, Fumie Kubota-Nakayama, Yuto Yamazaki, Yasuhiro Miki, Shuko Hata, Kazue Ise, Kumi Kikuchi, Ryo Morimoto, Fumitoshi Satoh, Hironobu Sasano

    Human pathology　77　88-97　2018/07

    DOI： 10.1016/j.humpath.2018.03.016 　

    ISSN： 0046-8177

    More details Close

    The tumor microenvironment plays pivotal roles in various human neoplasms. However, that of benign tumor, particularly hormone-secreting endocrine tumors, has remained virtually unknown. Therefore, we firstly attempted to analyze the tumor microenvironment of autonomous hormone-secreting adrenocortical adenomas. We first histologically evaluated 21 cortisol-producing adrenocortical adenoma (CPA) and 13 aldosterone-producing adrenocortical adenoma (APA) cases. Quantitative histologic analysis revealed that intratumoral immune cell infiltration (ICI) was more pronounced in CPAs than in APAs. We then evaluated the cytokine and chemokine profiles using polymerase chain reaction arrays in APAs and CPAs. Angiogenic chemokines, C-X-C motif chemokine ligand (CXCL) 1 and CXCL2, were significantly more abundant in CPAs than in APAs using subsequent quantitative polymerase chain reaction and immunohistochemical analyses. We then examined the vascular density between these 2 adenomas, and the density was significantly higher in overt CPAs than in APAs. Of particular interest, CXCL12-positive vessels were detected predominantly in CPAs, and their infiltrating immune cells were C-X-C motif chemokine receptor 4 (CXCR4) positive. These results above indicated that CXCL12-CXCR4 signaling could partly account for ICI detected predominantly in CPAs. We then further explored the etiology of ICI in CPAs by evaluating the senescence of tumor cells possibly caused by excessive cortisol in CPAs. The status of senescence markers, p16 and p21, was significantly more abundant in CPAs than in APAs. In addition, all CPA cases examined were positive for senescence-associated β-galactosidase. These results all indicated that exposure to local excessive cortisol could result in senescence of tumors cells and play essential roles in constituting the characteristic tissue microenvironment of CPAs.

  131. Roles of human epidermal growth factor receptor family in pulmonary lymphangioleiomyomatosis. International-journal Peer-reviewed

    Kobayashi K, Miki Y, Saito R, Adachi K, Seyama K, Okada Y, Sasano H

    Human pathology　81　121-130　2018/07

    DOI： 10.1016/j.humpath.2018.07.002 　

    ISSN： 0046-8177

    More details Close

    Lymphangioleiomyomatosis (LAM) is a rare and refractory disease that affects women of reproductive age. Several target therapies are used to manage LAM, but no curative modes of treatment have been reported yet. Therefore, in this study, we focused on targeting human epidermal growth factor receptor (HER) family proteins as a treatment strategy for LAM. In antibody array analysis, HER signaling was detected in the proteins extracted from LAM tissues. We then evaluated the expression of HER family members in 34 pulmonary LAM specimens using both immunohistochemistry and quantitative reverse-transcription polymerase chain reaction. Hierarchical clustering analysis was performed to classify the cases based on the immunohistochemistry results. Both epidermal growth factor receptor (EGFR) and HER4 were expressed in all 34 cases. HER3 was expressed in 25 of 34 cases, but HER2 was not expressed in any case. In addition, results of quantitative reverse-transcription polymerase chain reaction analysis confirmed the expression of EGFR and HER4 expression in LAM cells. Patients with HER3- or HER4-positive tissues were younger and had a history of pneumothorax. The cases were classified into 4 different clusters based on the results of hierarchical cluster analysis. One of these clusters was associated with EGFR, HER3, and HER4; the patients in this cluster were significantly younger and had a history of pneumothorax. These results indicated that HER family could contribute to the progression of pulmonary LAM, and treatments targeted against HER family might be effective for treating pulmonary LAM.

  132. The interplay of endocrine therapy, steroid pathways and therapeutic resistance: Importance of androgen in breast carcinoma Peer-reviewed

    Kiyoshi Takagi, Yasuhiro Miki, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    Molecular and Cellular Endocrinology　466　31-37　2018/05/05

    Publisher: Elsevier Ireland Ltd

    DOI： 10.1016/j.mce.2017.09.011 　

    ISSN： 1872-8057 0303-7207

    eISSN： 1872-8057

  133. 17β-hydroxysteroid dehydrogenase type 2 expression is induced by androgen signaling in endometrial cancer Peer-reviewed

    Chiaki Hashimoto, Yasuhiro Miki, Sota Tanaka, Kiyoshi Takagi, Misaki Fue, Zhulanqiqige Doe, Bin Li, Nobuo Yaegashi, Takashi Suzuki, Kiyoshi Ito

    International Journal of Molecular Sciences　19　(4)　2018/04/10

    Publisher: MDPI AG

    DOI： 10.3390/ijms19041139 　

    ISSN： 1422-0067 1661-6596

  134. 肺腺癌における癌関連線維芽細胞由来サイトカインによるPD-L1発現の誘導

    井上 千裕, 三木 康宏, 齊藤 涼子, 端 秀子, 渡辺 みか, 佐藤 郁郎, 岡田 克典, 笹野 公伸

    日本病理学会会誌　107　(1)　415-415　2018/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  135. 肺腺癌組織における癌関連線維芽細胞マーカーの比較検討

    玉槻 大輔, 井上 千裕, 三木 康宏, 齊藤 涼子, 岡田 克典, 笹野 公伸

    日本病理学会会誌　107　(1)　522-522　2018/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  136. ARHGAP15 in human breast carcinoma: A potent tumor suppressor regulated by androgens Peer-reviewed

    Kiyoshi Takagi, Yasuhiro Miki, Yoshiaki Onodera, Takanori Ishida, Mika Watanabe, Hironobu Sasano, Takashi Suzuki

    International Journal of Molecular Sciences　19　(3)　2018/03/10

    Publisher: MDPI AG

    DOI： 10.3390/ijms19030804 　

    ISSN： 1422-0067 1661-6596

    eISSN： 1422-0067

  137. Aromatase in normal and diseased liver Peer-reviewed

    Keigo Murakami, Shuko Hata, Yasuhiro Miki, Hironobu Sasano

    Hormone Molecular Biology and Clinical Investigation　2018/02/28

    Publisher: Walter de Gruyter GmbH

    DOI： 10.1515/hmbci-2017-0081 　

    ISSN： 1868-1891 1868-1883

  138. The significance of MMP-1 in EGFR-tki–resistant lung adenocarcinoma: Potential for therapeutic targeting Peer-reviewed

    Ryoko Saito, Yasuhiro Miki, Naoya Ishida, Chihiro Inoue, Masayuki Kobayashi, Shuko Hata, Hisafumi Yamada-Okabe, Yoshinori Okada, Hironobu Sasano

    International Journal of Molecular Sciences　19　(2)　2018/02/18

    Publisher: MDPI AG

    DOI： 10.3390/ijms19020609 　

    ISSN： 1422-0067 1661-6596

  139. Immunolocalization of OLFM4, LY6D and S100A7 related to distant metastasis in breast carcinoma. Peer-reviewed

    Mayama Akifumi, Suzuki Hiroyoshi, Takagi Kiyoshi, Onodera Yoshiaki, Watanabe Takanori, Miki Yasuhiro, Sakamoto Kazuhiro, Yoshida Ryuichi, Ishida Takanori, Sasano Hironobu, Suzuki Takashi

    CANCER SCIENCE　109　668　2018/01

    ISSN： 1349-7006

  140. The interaction of HER2 and carcinoembryonic antigen-related cell adhesion 6 are associated with efficacy of trastuzumab Peer-reviewed

    Iwabuchi Erina, Miki Yasuhiro, Sakurai Minako, Miyashita Minoru, Kanai Ayako, Ishida Takanori, Sasano Hironobu

    CANCER SCIENCE　109　660　2018/01

    ISSN： 1349-7006

  141. Effects of androgens on tumor-associated macrophages in breast carcinoma Peer-reviewed

    Sato Masayasu, Takagi Kiyoshi, Miki Yasuhiro, Ishida Takanori, Sasano Hironobu, Suzuki Takashi

    CANCER SCIENCE　109　669　2018/01

    ISSN： 1349-7006

  142. S100P and ezrin promoted transendothelial migration of carcinoma cells in triple negative breast cancer Peer-reviewed

    Kikuchi Kyoko, McNamara Keely M, Miki Yasuhiro, Iwabuchi Erina, Mitashita Minoru, Ishida Takanori, Ohuchi Noriaki, Sasano Hironobu

    CANCER SCIENCE　109　524　2018/01

    ISSN： 1349-7006

  143. Effects of cytokines derived from cancer-associated fibroblasts on androgen synthetic enzymes in estrogen receptor-negative breast carcinoma Peer-reviewed

    Kyoko Kikuchi, Keely May McNamara, Yasuhiro Miki, Ju-Yeon Moon, Man Ho Choi, Fumiya Omata, Minako Sakurai, Yoshiaki Onodera, Yoshiaki Rai, Yasuyo Ohi, Yasuaki Sagara, Minoru Miyashita, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    BREAST CANCER RESEARCH AND TREATMENT　166　(3)　709-723　2017/12

    DOI： 10.1007/s10549-017-4464-5 　

    ISSN： 0167-6806

    eISSN： 1573-7217

  144. Roles of Aryl Hydrocarbon Receptor in Aromatase-Dependent Cell Proliferation in Human Osteoblasts Peer-reviewed

    Yasuhiro Miki, Shuko Hata, Katsuhiko Ono, Takashi Suzuki, Kiyoshi Ito, Hiroyuki Kumamoto, Hironobu Sasano

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES　18　(10)　2017/10

    DOI： 10.3390/ijms18102159 　

    ISSN： 1422-0067

  145. Cytochrome c1 in ductal carcinoma in situ of breast associated with proliferation and comedo necrosis Peer-reviewed

    Mayuko Chishiki, Kiyoshi Takagi, Ai Sato, Yasuhiro Miki, Yuta Yamamoto, Akiko Ebata, Yukiko Shibahara, Mika Watanabe, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    CANCER SCIENCE　108　(7)　1510-1519　2017/07

    DOI： 10.1111/cas.13251 　

    ISSN： 1349-7006

  146. Cytochrome c1 in ductal carcinoma in situ of breast associated with proliferation and comedo necrosis Peer-reviewed

    Mayuko Chishiki, Kiyoshi Takagi, Ai Sato, Yasuhiro Miki, Yuta Yamamoto, Akiko Ebata, Yukiko Shibahara, Mika Watanabe, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    Cancer Science　108　(7)　1510-1519　2017/07/01

    Publisher: Blackwell Publishing Ltd

    DOI： 10.1111/cas.13251 　

    ISSN： 1349-7006 1347-9032

    eISSN： 1349-7006

  147. Interaction with adipocyte stromal cells induces breast cancer malignancy via S100A7 upregulation in breast cancer microenvironment Peer-reviewed

    Minako Sakurai, Yasuhiro Miki, Kiyoshi Takagi, Takashi Suzuki, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    BREAST CANCER RESEARCH　19　(1)　70　2017/06

    DOI： 10.1186/s13058-017-0863-0 　

    ISSN： 1465-542X

    eISSN： 1465-5411

  148. Retinoic Acid Receptor: A Potential Therapeutic Target in Retinoic Acid Treatment of Endometrial Cancer Peer-reviewed

    Keita Tsuji, Hiroki Utsunomiya, Yasuhiro Miki, Mayu Hanihara, Misaki Fue, Kiyoshi Takagi, Mitsuo Nishimoto, Fumihiko Suzuki, Nobuo Yaegashi, Takashi Suzuki, Kiyoshi Ito

    INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER　27　(4)　643-650　2017/05

    DOI： 10.1097/IGC.0000000000000995 　

    ISSN： 1048-891X

    eISSN： 1525-1438

  149. 乳癌におけるcarcinoembryonic antigen-related cell adhesion molecule 6および8の発現意義の検討

    岩渕 英里奈, 三木 康宏, 高木 清司, 小野寺 好明, 柴原 裕紀子, 鈴木 貴, 笹野 公伸

    日本病理学会会誌　106　(1)　347-347　2017/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  150. 肺腺癌におけるPD-L1発現に対する癌関連線維芽細胞の影響(The influence of cancer associated fibroblasts on PD-L1 expression in lung adenocarcinoma)

    井上 千裕, 三木 康宏, 齊藤 涼子, 端 秀子, 渡辺 みか, 佐藤 郁郎, 岡田 克典, 笹野 公伸

    日本病理学会会誌　106　(1)　409-409　2017/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  151. Aryl hydrocarbon receptor induced intratumoral aromatase in breast cancer Peer-reviewed

    Ryoko Saito, Yasuhiro Miki, Shuko Hata, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

    BREAST CANCER RESEARCH AND TREATMENT　161　(3)　399-407　2017/02

    DOI： 10.1007/s10549-016-4063-x 　

    ISSN： 0167-6806

    eISSN： 1573-7217

  152. In situ detection of estrogen receptor dimers in breast carcinoma cells in archival materials using proximity ligation assay (PLA) Peer-reviewed

    Erina Iwabuchi, Yasuhiro Miki, Katsuhiko Ono, Yoshiaki Onodera, Takashi Suzuki, Hisashi Hirakawa, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　165　(Pt B)　159-169　2017/01

    DOI： 10.1016/j.jsbmb.2016.05.022 　

    ISSN： 0960-0760

  153. In Situ Evaluation of Estrogen Receptor Dimers in Breast Carcinoma Cells: Visualization of Protein-Protein Interactions Peer-reviewed

    Erina Iwabuchi, Yasuhiro Miki, Katsuhiko Ono, Yoshiaki Onodera, Hironobu Sasano

    ACTA HISTOCHEMICA ET CYTOCHEMICA　50　(2)　85-93　2017

    DOI： 10.1267/ahc.17011 　

    ISSN： 0044-5991

    eISSN： 1347-5800

  154. Interaction with adipocyte stromal cells induces breast cancer malignancy via S100A7 upregulation in breast cancer microenvironment Peer-reviewed

    Minako Sakurai, Yasuhiro Miki, Kiyoshi Takagi, Takashi Suzuki, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    Breast Cancer Research　19　(1)　70　2017

    Publisher: BioMed Central Ltd.

    DOI： 10.1186/s13058-017-0863-0 　

    ISSN： 1465-542X 1465-5411

    eISSN： 1465-542X

  155. CITED2 in breast carcinoma as a potent prognostic predictor associated with proliferation, migration and chemoresistance Peer-reviewed

    Hiroyuki Minemura, Kiyoshi Takagi, Ai Sato, Hikaru Takahashi, Yasuhiro Miki, Yukiko Shibahara, Mika Watanabe, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    CANCER SCIENCE　107　(12)　1898-1908　2016/12

    DOI： 10.1111/cas.13081 　

    ISSN： 1347-9032

    eISSN： 1349-7006

  156. Analysis of multiple primary cancer autopsy cases associated with breast cancer: 2002-2010 Peer-reviewed

    Yukiko Shibahara, Yumi Sugawara, Yasuhiro Miki, Shuko Hata, Hiroaki Takahashi, Yasuhiro Nakamura, Takashi Suzuki, Noriaki Ohuchi, Ichiro Tsuji, Hironobu Sasano

    PATHOLOGY INTERNATIONAL　66　(12)　695-700　2016/12

    DOI： 10.1111/pin.12484 　

    ISSN： 1320-5463

    eISSN： 1440-1827

  157. 非小細胞肺癌における癌関連線維芽細胞のはたらき

    井上 千裕, 齊藤 涼子, 三木 康宏, 端 秀子, 笹野 公伸

    日本癌学会総会記事　75回　P-1331　2016/10

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  158. TACC2 (transforming acidic coiled-coil protein 2) in breast carcinoma as a potent prognostic predictor associated with cell proliferation Peer-reviewed

    Yoshiaki Onodera, Kiyoshi Takagi, Yasuhiro Miki, Ken-ichi Takayama, Yukiko Shibahara, Mika Watanabe, Takanori Ishida, Satoshi Inoue, Hironobu Sasano, Takashi Suzuki

    CANCER MEDICINE　5　(8)　1973-1982　2016/08

    DOI： 10.1002/cam4.736 　

    ISSN： 2045-7634

  159. In situ androgen and estrogen biosynthesis in endometrial cancer: focus on androgen actions and intratumoral production Peer-reviewed

    Kiyoshi Ito, Yasuhiro Miki, Takashi Suzuki, Keely May McNamara, Hironobu Sasano

    ENDOCRINE-RELATED CANCER　23　(7)　R323-R335　2016/07

    DOI： 10.1530/ERC-15-0470 　

    ISSN： 1351-0088

    eISSN： 1479-6821

  160. Steroid and xenobiotic receptor-mediated effects of bisphenol A on human osteoblasts Peer-reviewed

    Yasuhiro Miki, Shuko Hata, Shuji Nagasaki, Takashi Suzuki, Kiyoshi Ito, Hiroyuki Kumamoto, Hironobu Sasano

    LIFE SCIENCES　155　29-35　2016/06

    DOI： 10.1016/j.lfs.2016.05.013 　

    ISSN： 0024-3205

    eISSN： 1879-0631

  161. エナメル上皮腫における細胞内増殖シグナル伝達分子の検討

    及川 麻理子, 佐藤 郁郎, 三木 康宏, 纐纈 衆, 清水 良央, 熊本 裕行

    日本病理学会会誌　105　(1)　507-507　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  162. Immunohistochemical and genetic evaluations of epidermal growth factor receptor (EGFR) in oral squamous cell carcinoma Peer-reviewed

    Satoko Abe, Mariko Oikawa, Yasuhiro Miki, Yoshinaka Shimizu, Takashi Suzuki, Tetsu Takahashi, Hiroyuki Kumamoto

    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology　28　(2)　174-181　2016/03/01

    Publisher: Elsevier Ltd

    DOI： 10.1016/j.ajoms.2015.08.002 　

    ISSN： 2212-5558

  163. Nucleobindin 2 (NUCB2) in human endometrial carcinoma: a potent prognostic factor associated with cell proliferation and migration Peer-reviewed

    Kiyoshi Takagi, Yasuhiro Miki, Sota Tanaka, Chiaki Hashimoto, Mika Watanabe, Hironobu Sasano, Kiyoshi Ito, Takashi Suzuki

    ENDOCRINE JOURNAL　63　(3)　287-299　2016/03

    DOI： 10.1507/endocrj.EJ15-0490 　

    ISSN： 0918-8959

    eISSN： 1348-4540

  164. Intratumoral estrogen production and actions in luminal A type invasive lobular and ductal carcinomas Peer-reviewed

    Mayu Takagi, Yasuhiro Miki, Minoru Miyashita, Shuko Hata, Tomomi Yoda, Hisashi Hirakawa, Yasuaki Sagara, Yoshiaki Rai, Yasuyo Ohi, Kentaro Tamaki, Takanori Ishida, Takashi Suzuki, Noriaki Ouchi, Hironobu Sasano

    BREAST CANCER RESEARCH AND TREATMENT　156　(1)　45-55　2016/02

    DOI： 10.1007/s10549-016-3739-6 　

    ISSN： 0167-6806

    eISSN： 1573-7217

  165. Tumor microenvironment in invasive lobular carcinoma: possible therapeutic targets Peer-reviewed

    Saki Nakagawa, Yasuhiro Miki, Minoru Miyashita, Shuko Hata, Yayoi Takahashi, Yoshiaki Rai, Yasuaki Sagara, Yasuyo Ohi, Hisashi Hirakawa, Kentaro Tamaki, Takanori Ishida, Mika Watanabe, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

    BREAST CANCER RESEARCH AND TREATMENT　155　(1)　65-75　2016/01

    DOI： 10.1007/s10549-015-3668-9 　

    ISSN： 0167-6806

    eISSN： 1573-7217

  166. Intracrine steroid production and mammalian target of rapamycin pathways in pulmonary lymphangioleiomyomatosis Peer-reviewed

    Koko Adachi, Yasuhiro Miki, Ryoko Saito, Shuko Hata, Masanori Yamauchi, Yoshiki Mikami, Yoshinori Okada, Kuniaki Seyama, Takashi Kondo, Hironobu Sasano

    HUMAN PATHOLOGY　46　(11)　1685-1693　2015/11

    DOI： 10.1016/j.humpath.2015.02.019 　

    ISSN： 0046-8177

    eISSN： 1532-8392

  167. Abnormal expression of miR-1 in breast carcinoma as a potent prognostic factor Peer-reviewed

    Hiroyuki Minemura, Kiyoshi Takagi, Yasuhiro Miki, Yukiko Shibahara, Saki Nakagawa, Akiko Ebata, Mika Watanabe, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    CANCER SCIENCE　106　(11)　1642-1650　2015/11

    DOI： 10.1111/cas.12808 　

    ISSN： 1347-9032

    eISSN： 1349-7006

  168. Immunolocalization of thymidylate synthase as a favorable prognostic marker in estrogen receptor-positive breast carcinoma Peer-reviewed

    Kiyoshi Takagi, Yasuhiro Miki, Yasuhiro Nakamura, Hisashi Hirakawa, Yoichiro Kakugawa, Goro Amano, Mika Watanabe, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    HISTOLOGY AND HISTOPATHOLOGY　30　(10)　1223-1232　2015/10

    DOI： 10.14670/HH-11-619 　

    ISSN： 0213-3911

    eISSN： 1699-5848

  169. 11 beta-Prostaglandin F2 alpha, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer Peer-reviewed

    Tomomi Yoda, Kyoko Kikuchi, Yasuhiro Miki, Yoshiaki Onodera, Shuko Hata, Kiyoshi Takagi, Yasuhiro Nakamura, Hisashi Hirakawa, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano, Keely May McNamara

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　413　(C)　236-247　2015/09

    DOI： 10.1016/j.mce.2015.07.008 　

    ISSN： 0303-7207

  170. Androgen receptor and intracrine androgen signaling in endometrial carcinomas

    Yasuhiro Miki, Misaki Fue, Kiyoshi Takagi, Chiaki Hashimoto, Sota Tanaka, Takashi Suzuki, Kiyoshi Ito

    Receptors & Clinical Investigation　2　(4)　e853　2015/06/08

  171. KLF15 in breast cancer: a novel tumor suppressor? Peer-reviewed

    Tomomi Yoda, Keely May McNamara, Yasuhiro Miki, Yoshiaki Onodera, Kiyoshi Takagi, Yasuhiro Nakamura, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

    CELLULAR ONCOLOGY　38　(3)　227-235　2015/06

    DOI： 10.1007/s13402-015-0226-8 　

    ISSN： 2211-3428

    eISSN： 2211-3436

  172. Clinical significance of subtype classification in metastatic lymph nodes of breast cancer patients undergoing neoadjuvant chemotherapy Peer-reviewed

    Noriko Nemoto, Yukiko Shibahara, Hiroshi Tada, Keiko Uchida, Keely M. McNamara, Monica S. M. Chan, Mika Watanabe, Kentaro Tamaki, Minoru Miyashita, Yasuhiro Miki, Kohsuke Gonda, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS　30　(2)　E174-E183　2015/04

    DOI： 10.5301/jbm.5000128 　

    ISSN： 0393-6155

    eISSN： 1724-6008

  173. Androgen receptor and enzymes in lymph node metastasis and cancer reoccurrence in triple-negative breast cancer Peer-reviewed

    Keely May McNamara, Tomomi Yoda, Yasuhiro Miki, Yasuhiro Nakamura, Takashi Suzuki, Noriko Nemoto, Minoru Miyashita, Reiki Nishimura, Nobuyuki Arima, Kentaro Tamaki, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS　30　(2)　E184-E189　2015/04

    DOI： 10.5301/jbm.5000132 　

    ISSN： 0393-6155

    eISSN： 1724-6008

  174. Anterior gradient 2 is correlated with EGFR mutation in lung adenocarcinoma tissues Peer-reviewed

    Sodai Narumi, Yasuhiro Miki, Shuko Hata, Masahito Ebina, Mikiyoshi Saito, Kazushige Mori, Makoto Kobayashi, Takashi Suzuki, Erina Iwabuchi, Ikuro Sato, Makoto Maemondo, Chiaki Endo, Akira Inoue, Takashi Kondo, Hisafumi Yamada-Okabe, Masakazu Ichinose, Hironobu Sasano

    INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS　30　(2)　E234-E242　2015/04

    DOI： 10.5301/jbm.5000131 　

    ISSN： 0393-6155

    eISSN： 1724-6008

  175. Correlation of miRNA expression profiling in surgical pathology materials, with Ki-67, HER2, ER and PR in breast cancer patients Peer-reviewed

    Minako Sakurai, Mariko Masuda, Yasuhiro Miki, Hisashi Hirakawa, Takashi Suzuki, Hironobu Sasano

    INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS　30　(2)　E190-E199　2015/04

    DOI： 10.5301/jbm.5000141 　

    ISSN： 0393-6155

    eISSN： 1724-6008

  176. The role of 5 alpha-reductase type 1 associated with intratumoral dihydrotestosterone concentrations in human endometrial carcinoma Peer-reviewed

    Sota Tanaka, Yasuhiro Miki, Chiaki Hashimoto, Kiyoshi Takagi, Zhulanqiqige Doe, Bin Li, Nobuo Yaegashi, Takashi Suzuki, Kiyoshi Ito

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　401　(C)　56-64　2015/02

    DOI： 10.1016/j.mce.2014.11.022 　

    ISSN： 0303-7207

  177. Intratumoral androgen metabolism and actions in invasive lobular carcinoma of the breast Peer-reviewed

    Tomomi Yoda, Keely May McNamara, Yasuhiro Miki, Mayu Takagi, Yoshiaki Rai, Yasuyo Ohi, Yasuaki Sagara, Kentaro Tamaki, Hisashi Hirakawa, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

    CANCER SCIENCE　105　(11)　1503-1509　2014/11

    DOI： 10.1111/cas.12535 　

    ISSN： 1347-9032

    eISSN： 1349-7006

  178. Immunolocalization of Corticotropin-Releasing Hormone (CRH) and Its Receptors (CRHR1 and CRHR2) in Human Endometrial Carcinoma CRHR1 as a Potent Prognostic Factor Peer-reviewed

    Naoko Sato, Kiyoshi Takagi, Takashi Suzuki, Yasuhiro Miki, Sota Tanaka, Satoru Nagase, Hitoshi Warita, Shin Fukudo, Fumiko Sato, Hironobu Sasano, Kiyoshi Ito

    INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER　24　(9)　1549-1557　2014/11

    DOI： 10.1097/IGC.0000000000000269 　

    ISSN： 1048-891X

    eISSN： 1525-1438

  179. An activation of LC3A-mediated autophagy contributes to de novo and acquired resistance to EGFR tyrosine kinase inhibitors in lung adenocarcinoma Peer-reviewed

    Kaito Nihira, Yasuhiro Miki, Shinya Iida, Sodai Narumi, Katsuhiko Ono, Erina Iwabuchi, Kazue Ise, Kazushige Mori, Mikiyoshi Saito, Masahito Ebina, Ikuro Sato, Makoto Maemondo, Hisafumi Yamada-Okabe, Takashi Kondo, Hironobu Sasano

    JOURNAL OF PATHOLOGY　234　(2)　277-288　2014/10

    DOI： 10.1002/path.4354 　

    ISSN： 0022-3417

    eISSN： 1096-9896

  180. Immunohistochemical assessment of ATG7, LC3, and p62 in ameloblastomas Peer-reviewed

    Miwa Okada, Mariko Oikawa, Yasuhiro Miki, Yoshinaka Shimizu, Seishi Echigo, Tetsu Takahashi, Hiroyuki Kumamoto

    JOURNAL OF ORAL PATHOLOGY & MEDICINE　43　(8)　606-612　2014/09

    DOI： 10.1111/jop.12177 　

    ISSN： 0904-2512

    eISSN： 1600-0714

  181. Expression of steroidogenic enzymes in human sebaceous glands Peer-reviewed

    Takayoshi Inoue, Yasuhiro Miki, Shingo Kakuo, Akira Hachiya, Takashi Kitahara, Setsuya Aiba, Christos C. Zouboulis, Hironobu Sasano

    JOURNAL OF ENDOCRINOLOGY　222　(3)　301-312　2014/09

    DOI： 10.1530/JOE-14-0323 　

    ISSN： 0022-0795

    eISSN： 1479-6805

  182. Induction of adrenomedullin 2/intermedin expression by thyroid stimulating hormone in thyroid Peer-reviewed

    Shuji Nagasaki, Motoko Fukui, Satoko Asano, Katsuhiko Ono, Yasuhiro Miki, Sei-ichi Araki, Mitsui Isobe, Noriaki Nakashima, Kazuhiro Takahashi, Hironobu Sasano, Jun Sato

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　395　(1-2)　32-40　2014/09

    DOI： 10.1016/j.mce.2014.07.008 　

    ISSN： 0303-7207

  183. Immunohistochemical Evidence for the Association between Attenuated mTOR Signaling and Diffuse Alveolar Damage, A Fatal Lung Complication Peer-reviewed

    Ryoko Saito, Mitsuru Yanai, Yasuhiro Miki, Yuichiro Fukasawa, Junya Tominaga, Masahito Ebina, Hironobu Sasano

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　234　(1)　67-75　2014/09

    DOI： 10.1620/tjem.234.67 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  184. Tissue concentrations of estrogens and aromatase immunolocalization in interstitial pneumonia of human lung Peer-reviewed

    Shinji Taniuchi, Fumiyoshi Fujishima, Yasuhiro Miki, Keiko Abe, Yasuhiro Nakamura, Satoko Sato, Atsuko Kasajima, Misaki Fue, Kazuyuki Ishida, Mika Watanabe, Tomohiro Sakakibara, Sumiko Maeda, Takashi Suzuki, Hironobu Sasano

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　392　(1-2)　136-143　2014/07

    DOI： 10.1016/j.mce.2014.05.016 　

    ISSN： 0303-7207

  185. Tumor Suppressive Roles of ARHGAP15 in Human Breast Cancer Peer-reviewed

    Takagi Kiyoshi, Miki Yasuhiro, Onodera Yoshiaki, Nakamura Yasuhiro, Ishida Takanori, Sasano Hironobu, Suzuki Takashi

    ENDOCRINE REVIEWS　35　(3)　2014/06

    ISSN： 0163-769X

  186. The Interaction of Androgen Pathways with the Pioneer Factor FOXA1 in Triple Negative Breast Cancers Peer-reviewed

    McNamara Keely M, Nakamura Shota, Yoda Tomomi, Nurani Alif Meem, Miki Yasuhiro, Ishida Takanori, Ohuchi Noriaki, Sasano Hironobu

    ENDOCRINE REVIEWS　35　(3)　2014/06

    ISSN： 0163-769X

  187. 11 beta-Prostaglandin F2 alpha Produced By AKR1C3 Stimulates FP-Receptor and Contributes to Breast Cancer Progression Peer-reviewed

    Yoda Tomomi, McNamara Keely M, Miki Yasuhiro, Takagi Kiyoshi, Ishida Takanori, Suzuki Takashi, Ohuchi Noriaki, Sasano Hironobu

    ENDOCRINE REVIEWS　35　(3)　2014/06

    ISSN： 0163-769X

  188. Androgenic pathways in the progression of triple-negative breast carcinoma: a comparison between aggressive and non-aggressive subtypes Peer-reviewed

    Keely M. McNamara, Tomomi Yoda, Alif Meem Nurani, Yukiko Shibahara, Yasuhiro Miki, Lin Wang, Yasuhiro Nakamura, Koyu Suzuki, Yang Yang, Eriko Abe, Hisashi Hirakawa, Takashi Suzuki, Noriko Nemoto, Minoru Miyashita, Kentaro Tamaki, Takanori Ishida, Kristy A. Brown, Noriaki Ohuchi, Hironobu Sasano

    BREAST CANCER RESEARCH AND TREATMENT　145　(2)　281-293　2014/06

    DOI： 10.1007/s10549-014-2942-6 　

    ISSN： 0167-6806

    eISSN： 1573-7217

  189. An inhibition of p62/SQSTM1 caused autophagic cell death of several human carcinoma cells Peer-reviewed

    Kaito Nihira, Yasuhiro Miki, Katsuhiko Ono, Takashi Suzuki, Hironobu Sasano

    CANCER SCIENCE　105　(5)　568-575　2014/05

    DOI： 10.1111/cas.12396 　

    ISSN： 1347-9032

    eISSN： 1349-7006

  190. GATA4 immunolocalization in breast carcinoma as a potent prognostic predictor Peer-reviewed

    Kiyoshi Takagi, Takashi Moriguchi, Yasuhiro Miki, Yasuhiro Nakamura, Mika Watanabe, Takanori Ishida, Masayuki Yamamoto, Hironobu Sasano, Takashi Suzuki

    CANCER SCIENCE　105　(5)　600-607　2014/05

    DOI： 10.1111/cas.12382 　

    ISSN： 1347-9032

    eISSN： 1349-7006

  191. NRF2 immunolocalization in human breast cancer patients as a prognostic factor Peer-reviewed

    Yoshiaki Onodera, Hozumi Motohashi, Kiyoshi Takagi, Yasuhiro Miki, Yukiko Shibahara, Mika Watanabe, Takanori Ishida, Hisashi Hirakawa, Hironobu Sasano, Masayuki Yamamoto, Takashi Suzuki

    ENDOCRINE-RELATED CANCER　21　(2)　241-252　2014/04

    DOI： 10.1530/ERC-13-0234 　

    ISSN： 1351-0088

    eISSN： 1479-6821

  192. Activation of AXL and Antitumor Effects of a Monoclonal Antibody to AXL in Lung Adenocarcinoma Peer-reviewed

    Shinya Iida, Yasuhiro Miki, Takashi Suzuki, Kazushige Mori, Mikiyoshi Saito, Hiromichi Niikawa, Takashi Kondo, Hisafumi Yamada-Okabe, Hironobu Sasano

    ANTICANCER RESEARCH　34　(4)　1821-1827　2014/04

    ISSN： 0250-7005

    eISSN： 1791-7530

  193. Aryl Hydrocarbon Receptor in Breast Cancer-A Newly Defined Prognostic Marker Peer-reviewed

    Ryoko Saito, Yasuhiro Miki, Shuko Hata, Kiyoshi Takagi, Shinya Iida, Yuki Oba, Katsuhiko Ono, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

    HORMONES & CANCER　5　(1)　11-21　2014/02

    DOI： 10.1007/s12672-013-0160-z 　

    ISSN： 1868-8497

    eISSN： 1868-8500

  194. Amyloid precursor protein in human breast cancer: An androgen-induced gene associated with cell proliferation Peer-reviewed

    Takagi Kiyoshi, Ito Shigehiro, Miyazaki Toshiaki, Miki Yasuhiro, Shibahara Yukiko, Ishida Takanori, Watanabe Mika, Inoue Satoshi, Sasano Hironobu, Suzuki Takashi

    CANCER SCIENCE　104　(11)　1532-1538　2013/11

    DOI： 10.1111/cas.12239 　

    ISSN： 1349-7006

  195. Amyloid precursor protein in human breast cancer: An androgen-induced gene associated with cell proliferation Peer-reviewed

    Kiyoshi Takagi, Shigehiro Ito, Toshiaki Miyazaki, Yasuhiro Miki, Yukiko Shibahara, Takanori Ishida, Mika Watanabe, Satoshi Inoue, Hironobu Sasano, Takashi Suzuki

    Cancer Science　104　(11)　1532-1538　2013/11

    DOI： 10.1111/cas.12239 　

    ISSN： 1347-9032 1349-7006

    eISSN： 1349-7006

  196. Distinct nuclear receptor expression in stroma adjacent to breast tumors Peer-reviewed

    Kevin C. Knower, Ashwini L. Chand, Natalie Eriksson, Kiyoshi Takagi, Yasuhiro Miki, Hironobu Sasano, Jane E. Visvader, Geoffrey J. Lindeman, John W. Funder, Peter J. Fuller, Evan R. Simpson, Wayne D. Tilley, Peter J. Leedman, J. Dinny Graham, George E. O. Muscat, Christine L. Clarke, Colin D. Clyne

    BREAST CANCER RESEARCH AND TREATMENT　142　(1)　211-223　2013/11

    DOI： 10.1007/s10549-013-2716-6 　

    ISSN： 0167-6806

    eISSN： 1573-7217

  197. Identification of Androgen-responsive microRNAs and Androgen-related Genes in Breast Cancer Peer-reviewed

    Kiichiroh Nakano, Yasuhiro Miki, Shuko Hata, Akiko Ebata, Kiyoshi Takagi, Keely M. Mcnamara, Minako Sakurai, Mariko Masuda, Hisashi Hirakawa, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

    ANTICANCER RESEARCH　33　(11)　4811-4819　2013/11

    ISSN： 0250-7005

    eISSN： 1791-7530

  198. Hexokinase II in breast carcinoma: A potent prognostic factor associated with hypoxia-inducible factor-1 alpha and Ki-67 Peer-reviewed

    Sato-Tadano Akiko, Suzuki Takashi, Amari Masakazu, Takagi Kiyoshi, Miki Yasuhiro, Tamaki Kentaro, Watanabe Mika, Ishida Takanori, Sasano Hironobu, Ohuchi Noriaki

    CANCER SCIENCE　104　(10)　1380-1388　2013/10

    DOI： 10.1111/cas.12238 　

    ISSN： 1349-7006

  199. Hexokinase II in breast carcinoma: A potent prognostic factor associated with hypoxia-inducible factor-1α and Ki-67 Peer-reviewed

    Akiko Sato-Tadano, Takashi Suzuki, Masakazu Amari, Kiyoshi Takagi, Yasuhiro Miki, Kentaro Tamaki, Mika Watanabe, Takanori Ishida, Hironobu Sasano, Noriaki Ohuchi

    Cancer Science　104　(10)　1380-1388　2013/10

    DOI： 10.1111/cas.12238 　

    ISSN： 1347-9032 1349-7006

    eISSN： 1349-7006

  200. Androgen and androgen-metabolizing enzymes in metastasized lymph nodes of breast cancer Peer-reviewed

    Yukiko Shibahara, Yasuhiro Miki, Chikako Sakurada, Keiko Uchida, Shuko Hata, Keely McNamara, Tomomi Yoda, Kiyoshi Takagi, Yasuhiro Nakamura, Takashi Suzuki, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    HUMAN PATHOLOGY　44　(10)　2338-2345　2013/10

    DOI： 10.1016/j.humpath.2013.04.021 　

    ISSN： 0046-8177

  201. Proliferation and maturation of intratumoral blood vessels in non-small cell Lung cancer Peer-reviewed

    Samaneh Yazdani, Yasuhiro Miki, Kentaro Tamaki, Katsuhiko Ono, Erina Iwabuchi, Keiko Abe, Takashi Suzuki, Yasufumi Sato, Takashi Kondo, Hironobu Sasano

    HUMAN PATHOLOGY　44　(8)　1586-1596　2013/08

    DOI： 10.1016/j.humpath.2013.01.004 　

    ISSN： 0046-8177

  202. LMTK3 is implicated in endocrine resistance via multiple signaling pathways Peer-reviewed

    J. Stebbing, A. Filipovic, L. C. Lit, K. Blighe, A. Grothey, Y. Xu, Y. Miki, L. W. Chow, R. C. Coombes, H. Sasano, J. A. Shaw, G. Giamas

    Oncogene　32　(28)　3371-3380　2013/07/11

    DOI： 10.1038/onc.2012.343 　

    ISSN： 0950-9232 1476-5594

  203. Intratumoral localization and activity of 17 beta-hydroxysteroid dehydrogenase type 1 in non-small cell lung cancer: a potent prognostic factor Peer-reviewed

    Mohit K. Verma, Yasuhiro Miki, Keiko Abe, Takashi Suzuki, Hiromichi Niikawa, Satoshi Suzuki, Takashi Kondo, Hironobu Sasano

    JOURNAL OF TRANSLATIONAL MEDICINE　11　167　2013/07

    DOI： 10.1186/1479-5876-11-167 　

    ISSN： 1479-5876

  204. Aromatase in human liver and its diseases Peer-reviewed

    Shuko Hata, Yasuhiro Miki, Ryoko Saito, Kazuyuki Ishida, Mika Watanabe, Hironobu Sasano

    CANCER MEDICINE　2　(3)　305-315　2013/06

    DOI： 10.1002/cam4.85 　

    ISSN： 2045-7634

  205. Androgenic pathway in triple negative invasive ductal tumors: Its correlation with tumor cell proliferation Peer-reviewed

    McNamara Keely M, Yoda Tomomi, Miki Yasuhiro, Chanplakorn Niramol, Wongwaisayawan Sansanee, Incharoen Pimpin, Kongdan Youwanush, Wang Lin, Takagi Kiyoshi, Mayu Takagi, Nakamura Yasuhiro, Suzuki Takashi, Nemoto Noriko, Miyashita Minoru, Tamaki Kentaro, Ishida Takanori, Ohuchi Noriaki, Sasano Hironobu

    CANCER SCIENCE　104　(5)　639-646　2013/05

    DOI： 10.1111/cas.12121 　

    ISSN： 1349-7006

  206. Assessment of protein expression and gene status of human epidermal growth factor receptor (HER) family molecules in ameloblastomas Peer-reviewed

    Mariko Oikawa, Yasuhiro Miki, Yoshinaka Shimizu, Hiroyuki Kumamoto

    JOURNAL OF ORAL PATHOLOGY & MEDICINE　42　(5)　424-434　2013/05

    DOI： 10.1111/jop.12024 　

    ISSN： 0904-2512

    eISSN： 1600-0714

  207. Androgenic pathway in triple negative invasive ductal tumors: its correlation with tumor cell proliferation. International-journal Peer-reviewed

    Keely M McNamara, Tomomi Yoda, Yasuhiro Miki, Niramol Chanplakorn, Sansanee Wongwaisayawan, Pimpin Incharoen, Youwanush Kongdan, Lin Wang, Kiyoshi Takagi, Takagi Mayu, Yasuhiro Nakamura, Takashi Suzuki, Noriko Nemoto, Minoru Miyashita, Kentaro Tamaki, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    Cancer science　104　(5)　639-46　2013/05

    DOI： 10.1111/cas.12121 　

    ISSN： 1347-9032

    More details Close

    Triple negative breast cancer (TNBC) is defined by estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 negativity. Patients with TNBC frequently undergo an aggressive clinical course due to the unavailability of specific targeted therapies. Androgen receptor (AR) was reported to be expressed in up to 60% of TNBC cases but there have been controversies as to the roles of androgen signaling through AR in TNBC. Therefore, in this study, we analyzed the status of AR in combination with androgen synthesizing enzymes (5α-reductase type 1 (5αR1) and 17β-hydroxysteroid dehydrogenase type 5 (17βHSD5)] in order to further understand androgenic actions in TNBC. Androgen receptor, 5αR1, and 17βHSD5 were immunolocalized in a cohort of 203 TNBC patients from Thailand and Japan. We then correlated the findings with clinicopathological characteristics (age, stage, tumor diameter, lymph node invasion, metastatic spread, Ki-67 labeling index, disease-free survival, and overall survival) of the patients. Univariate analysis revealed that AR+/enzyme+ cases were associated with a significantly lower Ki-67 labeling index than AR-/enzyme- samples. Multivariate analysis indicated the presence of significant positive correlations between AR and enzyme status in tumor cells, and between tumor diameter, lymph node invasion, and distant metastasis. Significant negative correlations were also detected between Ki-67 labeling index and AR status (P = 0.04) or 5αR1 (P < 0.001). Cox proportional hazards analysis showed that Ki-67 labeling index and stage were the only factors predicting disease-free and overall survival of the patients, although univariate Kaplan-Meier analysis revealed AR/5αR1 negativity suggested a more adverse clinical course up to 80 months after surgery. These results suggest that the presence of androgen synthesizing pathways in addition to AR expression in tumor cells could confer a better clinical outcome through suppression of cell proliferation.

  208. Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma Peer-reviewed

    S. Iida, H. Kakinuma, Y. Miki, K. Abe, M. Sakurai, S. Suzuki, H. Niikawa, J. Akahira, T. Suzuki, H. Sasano

    British Journal of Cancer　108　(7)　1415-1424　2013/04/16

    DOI： 10.1038/bjc.2013.84 　

    ISSN： 0007-0920 1532-1827

  209. LIN28Bの乳癌における臨床病理学的意義

    櫻井 美奈子, 三木 康宏, 柴原 裕紀子, 平川 久, 鈴木 貴, 大内 憲明, 笹野 公伸

    日本内分泌学会雑誌　89　(1)　254-254　2013/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  210. 乳癌の転移リンパ節におけるアンドロゲンレセプターの発現解析

    柴原 裕紀子, 櫻田 千華子, 三木 康宏, 笹野 公伸

    日本内分泌学会雑誌　89　(1)　316-316　2013/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  211. BUB1 Immunolocalization in Breast Carcinoma: Its Nuclear Localization as a Potent Prognostic Factor of the Patients Peer-reviewed

    Kiyoshi Takagi, Yasuhiro Miki, Yukiko Shibahara, Yasuhiro Nakamura, Akiko Ebata, Mika Watanabe, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    HORMONES & CANCER　4　(2)　92-102　2013/04

    DOI： 10.1007/s12672-012-0130-x 　

    ISSN： 1868-8497

  212. Intratumoral Estrogen Concentration and Expression of Estrogen-Induced Genes in Male Breast Carcinoma: Comparison with Female Breast Carcinoma Peer-reviewed

    Kiyoshi Takagi, Takuya Moriya, Masafumi Kurosumi, Kimako Oka, Yasuhiro Miki, Akiko Ebata, Takashi Toshima, Shoji Tsunekawa, Hiroyuki Takei, Hisashi Hirakawa, Takanori Ishida, Shin-ichi Hayashi, Junichi Kurebayashi, Hironobu Sasano, Takashi Suzuki

    HORMONES & CANCER　4　(1)　1-11　2013/02

    DOI： 10.1007/s12672-012-0126-6 　

    ISSN： 1868-8497

    eISSN： 1868-8500

  213. Androgen receptor in triple negative breast cancer. (J Steroid Biochem Mol Biol)

    McNamara K M, Yoda T, Takagi K, Miki Y, Suzuki T, Sasano H

    J Steroid Biochem Mol Biol　133　66-76　2013/01

    DOI： 10.1016/j.jsbmb.2012.08.007 　

  214. Intratumoral concentration of estrogens and clinicopathological changes in ductal carcinoma in situ following aromatase inhibitor letrozole treatment Peer-reviewed

    Takagi K, Ishida T, Miki Y, Hirakawa H, Kakugawa Y, Amano G, Ebata A, Mori N, Nakamura Y, Watanabe M, Amari M, Ohuchi N, Sasano H, Suzuki T

    Br J Cancer　109　(1)　100-108　2013

    DOI： 10.1038/bjc.2013.284 　

    ISSN： 0007-0920

    eISSN： 1532-1827

  215. Immunohistochemical analysis of aromatase in metastatic lymph nodes of breast cancer Peer-reviewed

    Yukiko Shibahara, Yasuhiro Miki, Takanori Ishida, Yasuhiro Nakamura, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

    PATHOLOGY INTERNATIONAL　63　(1)　20-28　2013/01

    DOI： 10.1111/pin.12015 　

    ISSN： 1320-5463

  216. Phase two steroid metabolism and its roles in breast and prostate cancer patients Peer-reviewed

    Keely M. McNamara, Yasuhiro Nakamura, Yasuhiro Miki, Hironobu Sasano

    Frontiers in Endocrinology　4　116　2013

    DOI： 10.3389/fendo.2013.00116 　

    ISSN： 1664-2392

  217. Kruppel-like factor 5 in human breast carcinoma: a potent prognostic factor induced by androgens Peer-reviewed

    Kiyoshi Takagi, Yasuhiro Miki, Yoshiaki Onodera, Yasuhiro Nakamura, Takanori Ishida, Mika Watanabe, Satoshi Inoue, Hironobu Sasano, Takashi Suzuki

    ENDOCRINE-RELATED CANCER　19　(6)　741-750　2012/12

    DOI： 10.1530/ERC-12-0017 　

    ISSN： 1351-0088

  218. Carcinoembryonic antigen-related cell adhesion molecules as surrogate markers for EGFR inhibitor sensitivity in human lung adenocarcinoma. Peer-reviewed

    Kobayashi M, Miki Y, Ebina M, Abe K, Mori K, Narumi S, Suzuki T, Sato I, Maemondo M, Endo C, Inoue A, Kumamoto H, Kondo T, Yamada-Okabe H, Nukiwa T, Sasano H

    British journal of cancer　107　(10)　1745-1753　2012/11

    Publisher: 10

    DOI： 10.1038/bjc.2012.422 　

    ISSN： 0007-0920

    eISSN： 1532-1827

  219. Epigenetic mechanisms regulate the prostaglandin E receptor 2 in breast cancer Peer-reviewed

    Sarah Q. To, Kiyoshi Takagi, Yasuhiro Miki, Koyu Suzuki, Eriko Abe, Yang Yang, Hironobu Sasano, Evan R. Simpson, Kevin C. Knower, Colin D. Clyne

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　132　(3-5)　331-338　2012/11

    DOI： 10.1016/j.jsbmb.2012.07.007 　

    ISSN： 0960-0760

  220. Runt-related transcription factor 2 in human colon carcinoma: A potent prognostic factor associated with estrogen receptor Peer-reviewed

    Tomohiko Sase, Takashi Suzuki, Koh Miura, Kenichi Shiiba, Ikuro Sato, Yasuhiro Nakamura, Kiyoshi Takagi, Yoshiaki Onodera, Yasuhiro Miki, Mika Watanabe, Kazuyuki Ishida, Shinobu Ohnuma, Hiroyuki Sasaki, Ryuichiro Sato, Hideaki Karasawa, Chikashi Shibata, Michiaki Unno, Iwao Sasaki, Hironobu Sasano

    INTERNATIONAL JOURNAL OF CANCER　131　(10)　2284-2293　2012/11

    DOI： 10.1002/ijc.27525 　

    ISSN： 0020-7136

  221. Epigenetic mechanisms regulate the prostaglandin E receptor 2 in breast cancer. Peer-reviewed

    To SQ, Takagi K, Miki Y, Suzuki K, Abe E, Yang Y, Sasano H, Simpson ER, Knower KC, Clyne CD

    The Journal of steroid biochemistry and molecular biology　132　(3-5)　331-338　2012/11

    DOI： 10.1016/j.jsbmb.2012.07.007 　

    ISSN： 0960-0760

  222. Sex steroid synthesis in human skin in situ: The roles of aromatase and steroidogenic acute regulatory protein in the homeostasis of human skin Peer-reviewed

    Takayoshi Inoue, Yasuhiro Miki, Keiko Abe, Masahito Hatori, Masami Hosaka, Yoshiyuki Kariya, Shingo Kakuo, Tsutomu Fujimura, Akira Hachiya, Seijiro Honma, Setsuya Aiba, Hironobu Sasano

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　362　(1-2)　19-28　2012/10

    DOI： 10.1016/j.mce.2012.05.005 　

    ISSN： 0303-7207

  223. Co-expression of estrogen receptor beta and aromatase in Japanese lung cancer patients: Gender-dependent clinical outcome Peer-reviewed

    Mohit Kumar Verma, Yasuhiro Miki, Keiko Abe, Shuji Nagasaki, Hiromichi Niikawa, Satoshi Suzuki, Takashi Kondo, Hironobu Sasano

    LIFE SCIENCES　91　(15-16)　800-808　2012/10

    DOI： 10.1016/j.lfs.2012.08.029 　

    ISSN： 0024-3205

    eISSN： 1879-0631

  224. LIN28: A regulator of tumor-suppressing activity of let-7 microRNA in human breast cancer Peer-reviewed

    Minako Sakurai, Yasuhiro Miki, Mariko Masuda, Shuko Hata, Yukiko Shibahara, Hisashi Hirakawa, Takashi Suzuki, Hironobu Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　131　(3-5)　101-106　2012/09

    DOI： 10.1016/j.jsbmb.2011.10.007 　

    ISSN： 0960-0760

  225. The advantages of co-culture over mono cell culture in simulating in vivo environment

    Yasuhiro Miki, Katsuhiko Ono, Shuko Hata, Takashi Suzuki, Hiroyuki Kumamoto, Hironobu Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　131　(3-5)　68-75　2012/09

    DOI： 10.1016/j.jsbmb.2011.12.004 　

    ISSN： 0960-0760

  226. The advantages of co-culture over mono cell culture in simulating in vivo environment. Peer-reviewed

    Miki Y, Ono K, Hata S, Suzuki T, Kumamoto H, Sasano H

    The Journal of steroid biochemistry and molecular biology　131　(3-5)　68-75　2012/09

    DOI： 10.1016/j.jsbmb.2011.12.004 　

    ISSN： 0960-0760

  227. An induction of microRNA, miR-7 through estrogen treatment in breast carcinoma Peer-reviewed

    Mariko Masuda, Yasuhiro Miki, Shuko Hata, Kiyoshi Takagi, Minako Sakurai, Katsuhiko Ono, Koyu Suzuki, Yang Yang, Eriko Abe, Hisashi Hirakawa, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

    JOURNAL OF TRANSLATIONAL MEDICINE　10　S2　2012/09

    DOI： 10.1186/1479-5876-10-S1-S2 　

    ISSN： 1479-5876

  228. Oestrogen-induced genes in ductal carcinoma in situ: their comparison with invasive ductal carcinoma Peer-reviewed

    Akiko Ebata, Takashi Suzuki, Kiyoshi Takagi, Yasuhiro Miki, Yoshiaki Onodera, Yasuhiro Nakamura, Fumiyoshi Fujishima, Kazuyuki Ishida, Mika Watanabe, Kentaro Tamaki, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    ENDOCRINE-RELATED CANCER　19　(4)　485-496　2012/08

    DOI： 10.1530/ERC-11-0345 　

    ISSN： 1351-0088

  229. Aromatase in Colon Carcinoma Peer-reviewed

    Ryuichiro Sato, Takashi Suzuki, Yu Katayose, Koh Miura, Kenichi Shiiba, Yasuhiro Miki, Yukiko Kamogawa, Kuniharu Yamamoto, Takayuki Ii, Shinichi Egawa, Michiaki Unno, Hironobu Sasano

    ANTICANCER RESEARCH　32　(8)　3069-3075　2012/08

    ISSN： 0250-7005

    eISSN： 1791-7530

  230. MicroRNA-34b functions as a potential tumor suppressor in endometrial serous adenocarcinoma Peer-reviewed

    Eri Hiroki, Fumihiko Suzuki, Jun-ichi Akahira, Satoru Nagase, Kiyoshi Ito, Jun-ichi Sugawara, Yasuhiro Miki, Takashi Suzuki, Hironobu Sasano, Nobuo Yaegashi

    INTERNATIONAL JOURNAL OF CANCER　131　(4)　E395-E404　2012/08

    DOI： 10.1002/ijc.27345 　

    ISSN： 0020-7136

  231. Oestrogen-induced genes in ductal carcinoma in situ: their comparison with invasive ductal carcinoma. Peer-reviewed

    Ebata A, Suzuki T, Takagi K, Miki Y, Onodera Y, Nakamura Y, Fujishima F, Ishida K, Watanabe M, Tamaki K, Ishida T, Ohuchi N, Sasano H

    Endocrine-related cancer　19　(4)　485-496　2012/08

    DOI： 10.1530/ERC-11-0345 　

    ISSN： 1351-0088

  232. タバコ煙抽出液による核内受容体の活性化

    三木 康宏, 端 秀子, 熊本 裕行, 笹野 公伸

    The Journal of Toxicological Sciences　37　(Suppl.1)　S218-S218　2012/07

    Publisher: (一社)日本毒性学会

    ISSN： 0388-1350

    eISSN： 1880-3989

  233. Aromatase inhibitor treatment of breast cancer cells increases the expression of let-7f, a microRNA targeting CYP19A1 Peer-reviewed

    Yukiko Shibahara, Yasuhiro Miki, Yoshiaki Onodera, Shuko Hata, Monica S. M. Chan, Christopher C. P. Yiu, Tjing Y. Loo, Yasuhiro Nakamura, Jun-ichi Akahira, Takanori Ishida, Keiko Abe, Hisashi Hirakawa, Louis W. C. Chow, Takashi Suzuki, Noriaki Ouchi, Hironobu Sasano

    JOURNAL OF PATHOLOGY　227　(3)　357-366　2012/07

    DOI： 10.1002/path.4019 　

    ISSN： 0022-3417

  234. Estrogen receptor alpha and beta in esophageal squamous cell carcinoma Peer-reviewed

    Masashi Zuguchi, Yasuhiro Miki, Yoshiaki Onodera, Fumiyoshi Fujishima, Daisuke Takeyama, Hiroshi Okamoto, Go Miyata, Akira Sato, Susumu Satomi, Hironobu Sasano

    CANCER SCIENCE　103　(7)　1348-1355　2012/07

    DOI： 10.1111/j.1349-7006.2012.02288.x 　

    ISSN： 1347-9032

  235. 骨芽細胞における乳癌治療薬(アロマターゼ阻害剤)の影響

    三木 康宏, 端 秀子, 熊本 裕行, 笹野 公伸

    Clinical Calcium　22　(5)　751-751　2012/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  236. Accumulation of p62/SQSTM1 is associated with poor prognosis in patients with lung adenocarcinoma Peer-reviewed

    Daisuke Inoue, Takashi Suzuki, Yoichiro Mitsuishi, Yasuhiro Miki, Satoshi Suzuki, Shunichi Sugawara, Mika Watanabe, Akira Sakurada, Chiaki Endo, Akira Uruno, Hironobu Sasano, Takayuki Nakagawa, Kennichi Satoh, Nobuyuki Tanaka, Hiroshi Kubo, Hozumi Motohashi, Masayuki Yamamoto

    CANCER SCIENCE　103　(4)　760-766　2012/04

    DOI： 10.1111/j.1349-7006.2012.02216.x 　

    ISSN： 1347-9032

    eISSN： 1349-7006

  237. Melatonin suppresses aromatase expression and activity in breast cancer associated fibroblasts Peer-reviewed

    Kevin C. Knower, Sarah Q. To, Kiyoshi Takagi, Yasuhiro Miki, Hironobu Sasano, Evan R. Simpson, Colin D. Clyne

    BREAST CANCER RESEARCH AND TREATMENT　132　(2)　765-771　2012/04

    DOI： 10.1007/s10549-012-1953-4 　

    ISSN： 0167-6806

  238. エナメル上皮腫におけるerbBファミリー分子に関する検討

    及川 麻理子, 三木 康宏, 清水 良央, 熊本 裕行

    日本病理学会会誌　101　(1)　326-326　2012/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  239. Cytoplasmic estrogen receptor beta as a potential marker in human non-small cell lung carcinoma Peer-reviewed

    Mohit Kumar Verma, Yasuhiro Miki, Keiko Abe, Hiromichi Niikawa, Hironobu Sasano

    EXPERT OPINION ON THERAPEUTIC TARGETS　16　S91-S102　2012/03

    DOI： 10.1517/14728222.2011.630664 　

    ISSN： 1472-8222

  240. Phosphodiesterase type 9 (PDE9) in the human lower urinary tract: an immunohistochemical study Peer-reviewed

    Shuji Nagasaki, Youichi Nakano, Mariko Masuda, Katsuhiko Ono, Yasuhiro Miki, Yukiko Shibahara, Hironobu Sasano

    BJU INTERNATIONAL　109　(6)　934-940　2012/03

    DOI： 10.1111/j.1464-410X.2011.10429.x 　

    ISSN： 1464-4096

  241. Synergistic anti-tumor effects of RAD001 with MEK inhibitors in neuroendocrine tumors: A potential mechanism of therapeutic limitation of mTOR inhibitor Peer-reviewed

    Shinya Lida, Yasuhiro Miki, Katsuhiko Ono, Jun-Ichi Akahira, Yasuhiro Nakamura, Takashi Suzuki, Hironobu Sasano

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　350　(1)　99-106　2012/03

    DOI： 10.1016/j.mce.2011.11.024 　

    ISSN： 0303-7207

  242. Cytoplasmic estrogen receptor beta as a potential marker in human non-small cell lung carcinoma Peer-reviewed

    Mohit Kumar Verma, Yasuhiro Miki, Keiko Abe, Hiromichi Niikawa, Hironobu Sasano

    EXPERT OPINION ON THERAPEUTIC TARGETS　16　S91-S102　2012/03

    DOI： 10.1517/14728222.2011.630664 　

    ISSN： 1472-8222

  243. Cytochrome P450 19A1 (aromatase) in human liver and its diseases

    HATA Shuko, MIKI Yasuhiro, SASANO Hironobu

    Annual Meeting of the Japanese Society of Toxicology　39　AP-210　2012

    Publisher: The Japanese Society of Toxicology

    DOI： 10.14869/toxpt.39.2.0.AP-210.0 　

    More details Close

    Estrogens play important roles in the growth and invasion of estrogen-dependent human neoplasms. Aromatase overexpression has been reported to be expressed in hepatitis and hepatocellular carcinoma (HCC) compared to normal liver but its details in these hepatic diseases have remained unclear. Therefore in this study, we immunolocalized aromatase using immunohistochemistry in patients with liver cirrhosis, steatosis, hepatitis, HCC, and metastasis liver carcinoma (MLC) in order to study intrahepatic aromatase in details. Aromatase immunoreactivity was predominantly expressed in non-neoplastic hepatocytes around tumor cells. We then evaluated the effects of an interaction between hepatocytes and carcinoma cells on expression of aromatase mRNA in HepG2 using a co-culture systems. Aromatase mRNA levels in HepG2 were significantly increased by co-cultivation with all carcinoma cell lines examined. The same exon 1 splicing variant was used in aromatase expression in HepG2 cells regardless of carcinoma cell lines employed in the co-culture system. These findings all indicated that carcinoma cells, whether metastatic or primary, induced aromatase expression in adjacent normal hepatocytes through the soluble aromatase inducible factors in hepatic microenvironments.

  244. Correlation between mutagenicity test and CYPs expression profiles by normal human liver

    HATA Shuko, MIKI Yasuhiro, SASANO Hironobu

    Annual Meeting of the Japanese Society of Toxicology　39　P-11　2012

    Publisher: The Japanese Society of Toxicology

    DOI： 10.14869/toxpt.39.1.0.P-11.0 　

  245. Expression of aryl hydrocarbon receptor in bone tissues Peer-reviewed

    Yasuhiro Miki, Shuko Hata, Ryoko Saito, Katsuhiko Ono, Hironobu Sasano, Hiroyuki Kumamoto

    Interface Oral Health Science 2011　134-136　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_32 　

  246. Nucleobindin 2 in human breast carcinoma as a potent prognostic factor Peer-reviewed

    Shiho Suzuki, Kiyoshi Takagi, Yasuhiro Miki, Yoshiaki Onodera, Jun-ichi Akahira, Akiko Ebata, Takanori Ishida, Mika Watanabe, Hironobu Sasano, Takashi Suzuki

    CANCER SCIENCE　103　(1)　136-143　2012/01

    DOI： 10.1111/j.1349-7006.2011.02119.x 　

    ISSN： 1349-7006

  247. Immunohistochemical analysis of gastrin-releasing peptide receptor (GRPR) and possible regulation by estrogen receptor beta cx in human prostate carcinoma Peer-reviewed

    S. Nagasaki, Y. Nakamura, T. Maekawa, J. Akahira, Y. Mikii, T. Suzuki, S. Ishidoya, Y. Arai, H. Sasano

    NEOPLASMA　59　(2)　224-232　2012

    DOI： 10.4149/neo_2012_029 　

    ISSN： 0028-2685

  248. Sex steroid receptors in human lung diseases

    Mohit K. Verma, Yasuhiro Miki, Hironobu Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　127　(3-5)　216-222　2011/11

    DOI： 10.1016/j.jsbmb.2011.07.013 　

    ISSN： 0960-0760

  249. 肺癌におけるエストロゲンと喫煙の関係(Effects of smoking on intratumoral estrogen synthesis in lung cancer)

    三木 康宏, 鈴木 貴, 熊本 裕行, 笹野 公伸

    日本癌学会総会記事　70回　370-371　2011/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  250. ヒト食道扁平上皮癌におけるEstrogen receptor α and βの免疫組織化学的検討

    洞口正志, 三木康宏, 藤島史喜, 小野寺好明, 宮田剛, 里見進, 笹野公伸

    日本癌学会総会記事　70回　282-282　2011/09

  251. The role of estrogen-metabolizing enzymes and estrogen receptors in human epidermis Peer-reviewed

    Takayoshi Inoue, Yasuhiro Miki, Keiko Abe, Masahito Hatori, Masami Hosaka, Yoshiyuki Kariya, Shingo Kakuo, Tsutomu Fujimura, Akira Hachiya, Setsuya Aiba, Hironobu Sasano

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　344　(1-2)　35-40　2011/09

    DOI： 10.1016/j.mce.2011.06.015 　

    ISSN： 0303-7207

  252. Aromatase in human lung carcinoma

    Mohit K. Verma, Yasuhiro Miki, Hironobu Sasano

    STEROIDS　76　(8)　759-764　2011/07

    DOI： 10.1016/j.steroids.2011.02.020 　

    ISSN： 0039-128X

    eISSN： 1878-5867

  253. Suppression of estrogen actions in human lung cancer

    Yasuhiro Miki, Keiko Abe, Satoshi Suzuki, Takashi Suzuki, Hironobu Sasano

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　340　(2)　168-174　2011/07

    DOI： 10.1016/j.mce.2011.02.018 　

    ISSN： 0303-7207

  254. Steroid sulfatase and estrogen sulfotransferase in human carcinomas

    Takashi Suzuki, Yasuhiro Miki, Yasuhiro Nakamura, Kiyoshi Ito, Hironobu Sasano

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　340　(2)　148-153　2011/07

    DOI： 10.1016/j.mce.2010.11.001 　

    ISSN： 0303-7207

  255. 【乳癌治療-病態別治療の体系化】乳癌研究の最前線 Micro RNA

    笹野 公伸, 柴原 裕紀子, 三木 康宏

    カレントテラピー　29　(5)　425-430　2011/05

    Publisher: (株)ライフメディコム

    ISSN： 0287-8445

  256. Endocrine therapy after aromatase inhibitor therapy in breast cancer

    Mohit K Verma, Yasuhiro Miki, Hironobu Sasano

    Expert Review of Endocrinology and Metabolism　6　(3)　309-312　2011/05

    DOI： 10.1586/eem.11.21 　

    ISSN： 1744-6651 1744-8417

  257. 骨芽細胞におけるaryl hydrocarbon receptorを介したエストロゲン合成

    三木 康宏, 鈴木 貴, 熊本 裕行, 笹野 公伸

    日本内分泌学会雑誌　87　(1)　389-389　2011/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  258. Nudix-type motif 2 in human breast carcinoma: a potent prognostic factor associated with cell proliferation Peer-reviewed

    Kimako Oka, Takashi Suzuki, Yoshiaki Onodera, Yasuhiro Miki, Kiyoshi Takagi, Shuji Nagasaki, Jun-Ichi Akahira, Takanori Ishida, Mika Watanabe, Hisashi Hirakawa, Noriaki Ohuchi, Hironobu Sasano

    INTERNATIONAL JOURNAL OF CANCER　128　(8)　1770-1782　2011/04

    DOI： 10.1002/ijc.25505 　

    ISSN： 0020-7136

  259. Runx2 in human breast carcinoma: its potential roles in cancer progression Peer-reviewed

    Yoshiaki Onodera, Yasuhiro Miki, Takashi Suzuki, Kiyoshi Takagi, Jun-ichi Akahira, Takuya Sakyu, Mika Watanabe, Satoshi Inoue, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    CANCER SCIENCE　101　(12)　2670-2675　2010/12

    DOI： 10.1111/j.1349-7006.2010.01742.x 　

    ISSN： 1347-9032

  260. Novel classification based on immunohistochemistry combined with hierarchical clustering analysis in non-functioning neuroendocrine tumor patients Peer-reviewed

    Shinya Iida, Yasuhiro Miki, Katsuhiko Ono, Jun-ichi Akahira, Takashi Suzuki, Kazuyuki Ishida, Mika Watanabe, Hironobu Sasano

    CANCER SCIENCE　101　(10)　2278-2285　2010/10

    DOI： 10.1111/j.1349-7006.2010.01657.x 　

    ISSN： 1347-9032

  261. Immunolocalization of estrogen-producing and metabolizing enzymes in benign breast disease: Comparison with normal breast and breast carcinoma Peer-reviewed

    Yoshie Sasaki, Yasuhiro Miki, Hisashi Hirakawa, Yoshiaki Onodera, Kiyoshi Takagi, Jun-ichi Akahira, Seijiro Honma, Takanori Ishida, Mika Watanabe, Hironobu Sasano, Takashi Suzuki

    CANCER SCIENCE　101　(10)　2286-2292　2010/10

    DOI： 10.1111/j.1349-7006.2010.01673.x 　

    ISSN： 1349-7006

  262. ヒト乳癌におけるアロマターゼ阻害薬によるmiRNA発現の変動(MiRNA profile following aromatase inhibitor in breast cancer-potential difference between steroidal/non-steroidal agents)

    柴原 裕紀子, 三木 康宏, 小野 克彦, 赤平 純一, 阿部 佳子, 鈴木 貴, 笹野 公伸

    日本癌学会総会記事　69回　175-175　2010/08

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  263. Intratumoral Localization of Aromatase and Interaction between Stromal and Parenchymal Cells in the Non-Small Cell Lung Carcinoma Microenvironment Peer-reviewed

    Yasuhiro Miki, Takashi Suzuki, Keiko Abe, Satoshi Suzuki, Hiromichi Niikawa, Shinya Iida, Shuko Hata, Jun-ichi Akahira, Kazushige Mori, Dean B. Evans, Takashi Kondo, Hisafumi Yamada-Okabe, Hironobu Sasano

    CANCER RESEARCH　70　(16)　6659-6669　2010/08

    DOI： 10.1158/0008-5472.CAN-09-4653 　

    ISSN： 0008-5472

    eISSN： 1538-7445

  264. Increased intratumoral androgens in human breast carcinoma following aromatase inhibitor exemestane treatment Peer-reviewed

    Kiyoshi Takagi, Yasuhiro Miki, Shuji Nagasaki, Hisashi Hirakawa, Yoshiaki Onodera, Jun-ichi Akahira, Takanori Ishida, Mika Watanabe, Izo Kimijima, Shin-ichi Hayashi, Hironobu Sasano, Takashi Suzuki

    ENDOCRINE-RELATED CANCER　17　(2)　415-430　2010/06

    DOI： 10.1677/ERC-09-0257 　

    ISSN： 1351-0088

  265. Androgens in human breast carcinoma

    Takashi Suzuki, Yasuhiro Miki, Kiyoshi Takagi, Hisashi Hirakawa, Takuya Moriya, Noriaki Ohuchi, Hironobu Sasano

    MEDICAL MOLECULAR MORPHOLOGY　43　(2)　75-81　2010/06

    DOI： 10.1007/s00795-010-0494-3 　

    ISSN： 1860-1480

  266. Steroid and Xenobiotic Receptor (SXR) as a possible prognostic marker in epithelial ovarian cancer Peer-reviewed

    Xiaoni Yue, Jun-ichi Akahira, Hiroki Utsunomiya, Yasuhiro Miki, Naomi Takahashi, Hitoshi Niikura, Kiyoshi Ito, Hironobu Sasano, Kunihiro Okamura, Nobuo Yaegashi

    PATHOLOGY INTERNATIONAL　60　(5)　400-406　2010/05

    DOI： 10.1111/j.1440-1827.2010.02546.x 　

    ISSN： 1320-5463

    eISSN： 1440-1827

  267. Increased estrogen sulfatase (STS) and 17 beta-hydroxysteroid dehydrogenase type 1(17 beta-HSD1) following neoadjuvant aromatase inhibitor therapy in breast cancer patients Peer-reviewed

    Niramol Chanplakorn, Pongsthorn Chanplakorn, Takashi Suzuki, Katsuhiko Ono, Monica S. M. Chan, Yasuhiro Miki, Shigetoyo Saji, Takayuki Ueno, Masakazu Toi, Hironobu Sasano

    BREAST CANCER RESEARCH AND TREATMENT　120　(3)　639-648　2010/04

    DOI： 10.1007/s10549-010-0785-3 　

    ISSN： 0167-6806

  268. 皮膚扁平上皮癌および皮膚上皮内癌におけるエストロゲン受容体とアロマターゼの発現の検討

    森美紀, 阿部佳子, 小野克彦, 三木康宏, 赤平純一, 笹野公伸

    日本病理学会会誌　99　(1)　243-243　2010/03

  269. エストロゲン環境の撹乱を介した喫煙の肺癌プログレッション作用

    三木康宏, 鈴木貴, 阿部佳子, 鈴木聡, 笹野公伸

    日本内分泌学会雑誌　86　(1)　168-168　2010/03

  270. Cytochrome 3A and 2E1 in human liver tissue: Individual variations among normal Japanese subjects. Peer-reviewed

    Hata S, Miki Y, Fujishima F, Sato R, Okaue A, Abe K, Ishida K, Akahira J, Unno M, Sasano H

    Life sciences　86　(11-12)　393-401　2010/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.lfs.2010.01.011 　

    ISSN： 0024-3205

  271. Breast cancer aromatase expression evaluated by the novel antibody 677: correlations to intra-tumor estrogen levels and hormone receptor status.

    Geisler Jurgen, Suzuki Takashi, Helle Hildegunn, Miki Yasuhiro, Nagasaki Shuji, Duong Nhat K, Ekse Dagfinn, Aas Turid, Evans Dean B, Lonning Per E, Sasano Hironobu

    J Steroid Biochem Mol Biol　118　(4月5日)　237-241　2010/02/28

    DOI： 10.1016/j.jsbmb.2009.10.010 　

  272. Aromatase and in situ estrogen production in DCIS (ductal carcinoma in situ) of human breast. Peer-reviewed

    Sasano Hironobu, Miki Yasuhiro, Shibuya Rie, Suzuki Takashi

    J Steroid Biochem Mol Biol　118　(4月5日)　242-245　2010/02/28

    DOI： 10.1016/j.jsbmb.2009.09.008 　

  273. Steroid and xenobiotic receptor in human esophageal squamous cell carcinoma: a potent prognostic factor. Peer-reviewed

    Takeyama Daisuke, Miki Yasuhiro, Fujishima Fumiyoshi, Suzuki Takashi, Akahira Jun-Ichi, Hata Shuko, Miyata Go, Satomi Susumu, Sasano Hironobu

    Cancer Sci　101　(2)　543-549　2010/02

    Publisher: Wiley

    DOI： 10.1111/j.1349-7006.2009.01380.x 　

    ISSN： 1347-9032

    eISSN： 1349-7006

  274. Highly concordant coexpression of aromatase and estrogen receptor beta in non-small cell lung cancer Peer-reviewed

    Keiko Abe, Yasuhiro Miki, Katsuhiko Ono, Miki Mori, Hideaki Kakinuma, Yuki Kou, Nobutaka Kudo, Masashi Koguchi, Hiromichi Niikawa, Satoshi Suzuki, Dean B. Evans, Shunichi Sugawara, Takashi Suzuki, Hironobu Sasano

    HUMAN PATHOLOGY　41　(2)　190-198　2010/02

    DOI： 10.1016/j.humpath.2009.07.010 　

    ISSN： 0046-8177

  275. Epitope Characterization of an Aromatase Monoclonal Antibody Suitable for the Assessment of Intratumoral Aromatase Activity Peer-reviewed

    Yanyan Hong, Hongzhi Li, Jingjing Ye, Yasuhiro Miki, Yate-Ching Yuan, Hironobu Sasano, Dean B. Evans, Shiuan Chen

    PLOS ONE　4　(11)　e8050-e8050　2009/11

    DOI： 10.1371/journal.pone.0008050 　

    ISSN： 1932-6203

  276. In situ estrogen production and its regulation in human breast carcinoma: From endocrinology to intracrinology

    Hironobu Sasano, Yasuhiro Miki, Shuji Nagasaki, Takashi Suzuki

    PATHOLOGY INTERNATIONAL　59　(11)　777-789　2009/11

    DOI： 10.1111/j.1440-1827.2009.02444.x 　

    ISSN： 1320-5463

    eISSN： 1440-1827

  277. Local Biosynthesis of Estrogen in Human Endometrial Carcinoma through Tumor-Stromal Cell Interactions Peer-reviewed

    Naomi Takahashi-Shiga, Hiroki Utsunomiya, Yasuhiro Miki, Satoru Nagase, Rika Kobayashi, Mitsuyo Matsumoto, Hitoshi Niikura, Kiyoshi Ito, Nobuo Yaegashi

    CLINICAL CANCER RESEARCH　15　(19)　6028-6034　2009/10

    DOI： 10.1158/1078-0432.CCR-09-1013 　

    ISSN： 1078-0432

    eISSN： 1557-3265

  278. Transcriptional regulation of 17β-hydroxysteroid dehydrogenase type 12 by SREBP-1 Peer-reviewed

    Shuji Nagasaki, Yasuhiro Miki, Jun-ichi Akahira, Takashi Suzuki, Hironobu Sasano

    Molecular and Cellular Endocrinology　307　(1-2)　163-168　2009/08/13

    DOI： 10.1016/j.mce.2009.04.002 　

    ISSN： 0303-7207

  279. 神経内分泌腫瘍に対するmTOR阻害剤およびMEK阻害剤の効果に関する基礎的検討

    飯田慎也, 三木康宏, 小野克彦, 笹野公伸

    日本癌学会総会記事　68回　355-355　2009/08

  280. 乳癌におけるRunx2発現とその意義の免疫組織化学的検討

    小野寺好明, 鈴木貴, 佐久拓弥, 森美紀, 三木康宏, 赤平純一, 笹野公伸

    日本癌学会総会記事　68回　322-322　2009/08

  281. 乳癌におけるアロマターゼ阻害剤術前投与に伴うアンドロゲン作用の増大

    高木清司, 長崎修治, 小野寺好明, 三木康宏, 赤平純一, 小野克彦, 平川久, 笹野公伸, 鈴木貴

    日本癌学会総会記事　68回　322-322　2009/08

  282. 肺癌組織における癌微小環境 癌細胞増殖に対する間質細胞の影響

    三木康宏, 森一茂, 岡部尚文, 阿部佳子, 赤平純一, 鈴木貴, 笹野公伸

    日本癌学会総会記事　68回　321-321　2009/08

  283. ヒト非小細胞肺癌におけるsteroid sulfataseの発現

    柿沼秀明, 三木康宏, 阿部佳子, 鈴木聡, 赤平純一, 鈴木貴, 笹野公伸

    日本癌学会総会記事　68回　319-319　2009/08

  284. アロマターゼ阻害剤とmicroRNA

    柴原裕紀子, 三木康宏, 小野克彦, 赤平純一, 阿部佳子, 鈴木貴, 笹野公伸

    日本癌学会総会記事　68回　147-147　2009/08

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  285. 子宮内膜癌におけるmiR34bの発現およびDNAメチル化による制御

    山崎有人, 鈴木史彦, 廣木恵理, 三木康宏, 赤平純一, 笹野公伸

    日本癌学会総会記事　68回　146-146　2009/08

  286. Aromatase expression and outcomes in the P024 neoadjuvant endocrine therapy trial Peer-reviewed

    Matthew J. Ellis, William R. Miller, Yu Tao, Dean B. Evans, Hilary A. Chaudri Ross, Yasuhiro Miki, Takashi Suzuki, Hironobu Sasano

    BREAST CANCER RESEARCH AND TREATMENT　116　(2)　371-378　2009/07

    DOI： 10.1007/s10549-008-0161-8 　

    ISSN： 0167-6806

  287. ヒト骨芽細胞における内分泌撹乱物質の影響 Steroid and xenobiotic receptorを介したbisphenol Aの作用

    三木康宏, 端秀子, 笹野公伸

    Clinical Calcium　19　(6)　860-860　2009/05

  288. 神経内分泌腫瘍に対するmTOR阻害剤の影響とソマトスタチン誘導体の併用効果に関する検討

    飯田慎也, 三木康宏, 小野克彦, 笹野公伸

    日本内分泌学会雑誌　85　(1)　352-352　2009/04

  289. 乳癌における17β-hydroxysteroid dehydrogenase type12の機能に関する検討

    長崎修治, 鈴木貴, 三木康宏, 赤平純一, 笹野公伸

    日本内分泌学会雑誌　85　(1)　351-351　2009/04

  290. 卵巣癌におけるsteroid xenobiotic receptorの発現と臨床病理学的因子との関連についての検討

    楽暁ジ, 赤平純一, 三木康宏, 宇都宮裕貴, 伊藤潔, 笹野公伸, 八重樫伸生

    日本内分泌学会雑誌　85　(1)　321-321　2009/04

  291. ヒト肺癌におけるsteroid sulfataseの発現

    三木康宏, 柿沼秀明, 阿部佳子, 鈴木聡, 赤平純一, 鈴木貴, 笹野公伸

    日本内分泌学会雑誌　85　(1)　321-321　2009/04

  292. Chicken ovalbumin upstream promoter transcription factor II in human breast carcinoma: Possible regulator of lymphangiogenesis via vascular endothelial growth factor-C expression Peer-reviewed

    Shuji Nagasaki, Takashi Suzuki, Yasuhiro Miki, Jun-ichi Akahira, Hirotaka Shibata, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

    CANCER SCIENCE　100　(4)　639-645　2009/04

    DOI： 10.1111/j.1349-7006.2008.01078.x 　

    ISSN： 1347-9032

  293. An Analysis of Potential Surrogate Markers of Target-Specific Therapy in Archival Materials of Adrenocortical Carcinoma Peer-reviewed

    Megumi Nakamura, Yasuhiro Miki, Jun-ichi Akahira, Ryo Morimoto, Fumitoshi Satoh, Shigeto Ishidoya, Yoichi Arai, Takashi Suzuki, Yutaka Hayashi, Hironobu Sasano

    ENDOCRINE PATHOLOGY　20　(1)　17-23　2009/03

    DOI： 10.1007/s12022-009-9058-2 　

    ISSN： 1046-3976

  294. Intracrinology of sex steroids in ductal carcinoma in situ (DCIS) of human breast: Comparison to invasive ductal carcinoma (IDC) and non-neoplastic breast

    Yasuhiro Miki, Takashi Suzuki, Hironobu Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　114　(1-2)　68-71　2009/03

    DOI： 10.1016/j.jsbmb.2008.12.021 　

    ISSN： 0960-0760

  295. ステロイドサルファターゼとエストロゲンスルフォトランスフェラーゼは大腸癌腫瘍内エストロゲン濃度を制御し、また、予後規定因子となる Peer-reviewed

    佐藤龍一郎, 鈴木貴, 山本久仁治, 三浦康, 椎葉健一, 立野紘雄, 三木康宏, 井伊貴幸, 赤平純一, 元井冬彦, 力山敏樹, 片寄友, 江川新一, 笹野公伸, 海野倫明

    日本外科学会雑誌　110　(臨増2)　448-448　2009/02

  296. Steroid Sulfatase and Estrogen Sulfotransferase in Colon Carcinoma: Regulators of Intratumoral Estrogen Concentrations and Potent Prognostic Factors Peer-reviewed

    Ryuichiro Sato, Takashi Suzuki, Yu Katayose, Koh Miura, Kenichi Shiiba, Hiroo Tateno, Yasuhiro Miki, Junichi Akahira, Yukiko Kamogawa, Shuji Nagasaki, Kuniharu Yamamoto, Takayuki Ii, Shinichi Egawa, Dean B. Evans, Michiaki Unno, Hironobu Sasano

    CANCER RESEARCH　69　(3)　914-922　2009/02

    DOI： 10.1158/0008-5472.CAN-08-0906 　

    ISSN： 0008-5472

    eISSN： 1538-7445

  297. Comparative effects of raloxifene, tamoxifen and estradiol on human osteoblasts in vitro: Estrogen receptor dependent or independent pathways of raloxifene Peer-reviewed

    Yasuhiro Miki, Takashi Suzuki, Shuji Nagasaki, Shuko Hata, Jun-ichi Akahira, Hironobu Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　113　(3-5)　281-289　2009/02

    DOI： 10.1016/j.jsbmb.2009.01.010 　

    ISSN： 0960-0760

  298. 17 beta-Hydroxysteroid Dehydrogenase Type 12 in Human Breast Carcinoma: A Prognostic Factor via Potential Regulation of Fatty Acid Synthesis Peer-reviewed

    Shuji Nagasaki, Takashi Suzuki, Yasuhiro Miki, Jun-ichi Akahira, Kunio Kitada, Takanori Ishida, Hiroshi Handa, Noriaki Ohuchi, Hironobu Sasano

    CANCER RESEARCH　69　(4)　1392-1399　2009/02

    DOI： 10.1158/0008-5472.CAN-08-0821 　

    ISSN： 0008-5472

  299. New Developments in Intracrinology of Human Breast Cancer Estrogen Sulfatase and Sulfotransferase

    Hironobu Sasano, Shuji Nagasaki, Yasuhiro Miki, Takashi Suzuki

    STEROID ENZYMES AND CANCER　1155　76-79　2009

    DOI： 10.1111/j.1749-6632.2008.03683.x 　

    ISSN： 0077-8923

  300. 17 beta-Hydroxysteroid Dehydrogenases in Human Breast Cancer

    Shuji Nagasaki, Yasuhiro Miki, Jun-ichi Akahira, Takashi Suzuki, Hironobu Sasano

    STEROID ENZYMES AND CANCER　1155　25-32　2009

    DOI： 10.1111/j.1749-6632.2008.03682.x 　

    ISSN： 0077-8923

  301. Intratumoral estrogen production in breast carcinoma: significance of aromatase

    Takashi Suzuki, Yasuhiro Miki, Noriaki Ohuchi, Hironobu Sasano

    BREAST CANCER　15　(4)　270-277　2008/10

    DOI： 10.1007/s12282-008-0062-z 　

    ISSN： 1340-6868

    eISSN： 1880-4233

  302. 大腸癌におけるステロイドサルファターゼとエストロゲンスルフォトランスフェラーゼの発現意義

    佐藤龍一郎, 鈴木貴, 片寄友, 三浦康, 椎葉健一, 立野紘雄, 三木康宏, 赤平純一, 山本久仁治, 井伊貴幸, 江川新一, 海野倫明, 笹野公伸

    日本癌学会総会記事　67回　197-197　2008/09

  303. Intratumoral estrogens and estrogen receptors in human non-small cell lung carcinoma Peer-reviewed

    Hiromichi Niikawa, Takashi Suzuki, Yasuhiro Miki, Satoshi Suzuki, Shuji Nagasaki, Junichi Akahira, Seijiro Honma, Dean B. Evans, Shin-ichi Hayashi, Takashi Kondo, Hironobu Sasano

    CLINICAL CANCER RESEARCH　14　(14)　4417-4426　2008/07

    DOI： 10.1158/1078-0432.CCR-07-1950 　

    ISSN： 1078-0432

  304. ヒト肝組織における薬物代謝酵素発現パターンの個人差の解析

    端秀子, 三木康宏, 藤島史喜, 佐藤龍一郎, 海野倫明, 笹野公伸

    The Journal of Toxicological Sciences　33　(Suppl.)　S155-S155　2008/06

  305. 骨芽細胞に対するエストロゲン受容体作動薬の直接作用の比較 Invited

    三木康宏, 端秀子, 長崎修治, 赤平純一, 鈴木貴, 笹野公伸

    Clinical Calcium　18　(7)　1032-1032　2008/06

  306. 非小細胞肺癌における組織内Estradiol濃度とEstrogen receptor発現の関係 Peer-reviewed

    新井川弘道, 鈴木貴, 三木康宏, 鈴木聡, 近藤丘, 笹野公伸

    日本外科学会雑誌　109　(臨増2)　468-468　2008/04

  307. 乳癌における17beta-hydroxysteroid dehydrogenase type12の機能に関する検討 Peer-reviewed

    長崎修治, 三木康宏, 赤平純一, 鈴木貴, 笹野公伸

    日本内分泌学会雑誌　84　(1)　284-284　2008/04

  308. ヒト上皮性卵巣癌におけるSteroid sulfatase(STS)およびEstrogen sulfotransferase(EST)の発現について Peer-reviewed

    赤平純一, 鈴木貴, 鈴木史彦, 三木康宏, 伊藤潔, 八重樫伸生, 笹野公伸

    日本内分泌学会雑誌　84　(1)　284-284　2008/04

  309. ヒト肺癌におけるaromataseの発現 Peer-reviewed

    三木康宏, 新井川弘道, 鈴木貴, 青木貴央, 端秀子, 長崎修治, 阿部佳子, 赤平純一, 鈴木聡, 近藤丘, 笹野公伸

    日本内分泌学会雑誌　84　(1)　284-284　2008/04

  310. 食道扁平上皮癌におけるSXR、MDR1、MRP2の免疫組織化学的検討 Peer-reviewed

    武山大輔, 藤島史喜, 三木康宏, 赤平純一, 鈴木貴, 宮崎修吉, 笹野公伸

    日本病理学会会誌　97　(1)　285-285　2008/03

  311. Sex steroid receptors expression and hormone-induced cell proliferation in human osteosarcoma Peer-reviewed

    Osamu Dohi, Masahito Hatori, Takashi Suzuki, Katsuhiko Ono, Masami Hosaka, Jun-ichi Akahira, Yasuhiro Miki, Shuji Nagasaki, Eiji Itoi, Hironobu Sasano

    CANCER SCIENCE　99　(3)　518-523　2008/03

    DOI： 10.1111/j.1349-7006.2007.00673.x 　

    ISSN： 1347-9032

  312. Intratumoral concentration of sex steroids and expression of sex steroid-producing enzymes in ductal carcinoma in situ of human breast Peer-reviewed

    Rie Shibuya, Takashi Suzuki, Yasuhiro Miki, Kimako Yoshida, Takuya Moriya, Katsuhiko Ono, Jun-ichi Akahira, Takanori Ishida, Hisashi Hirakawa, Dean B. Evans, Hironobu Sasano

    ENDOCRINE-RELATED CANCER　15　(1)　113-124　2008/03

    DOI： 10.1677/ERC-07-0092 　

    ISSN： 1351-0088

  313. Review: Aromatase in human breast carcinoma as a key regulator of intratumoral sex steroid concentrations

    Takashi Suzuki, Yasuhiro Miki, Jun-Ichi Akahira, Takuya Moriya, Noriaki Ohuchi, Hironobu Sasano

    Endocrine Journal　55　(3)　455-463　2008

    DOI： 10.1507/endocrj.K07E-053 　

    ISSN： 0918-8959 1348-4540

  314. Decreased expression of RIZ1 and its clinicopathological significance in epithelial ovarian carcinoma: Correlation with epigenetic inactivation by aberrant DNA methylation Peer-reviewed

    Jun-ichi Akahira, Fumihiko Suzuki, Takashi Suzuki, Ikumi Miura, Noriko Kamogawa, Yasuhiro Miki, Kiyoshi Ito, Nobuo Yaegashi, Hironobu Sasano

    PATHOLOGY INTERNATIONAL　57　(11)　725-733　2007/11

    DOI： 10.1111/j.1440-1827.2007.02169.x 　

    ISSN： 1320-5463

  315. Aromatase inhibitor and bone

    Yasuhiro Miki, Takashi Suzuki, Hironobu Sasano

    BIOMEDICINE & PHARMACOTHERAPY　61　(9)　540-542　2007/10

    DOI： 10.1016/j.biopha.2007.08.011 　

    ISSN： 0753-3322

  316. Intracrinology of estrogens and androgens in breast carcinoma. Peer-reviewed

    Sasano H, Suzuki T, Miki Y, Moriya T

    J Steroid Biochem Mol Biol　108　?-185　2007/09/06

    DOI： 10.1016/j.jsbmb.2007.09.012 　

  317. 骨芽細胞に対するSteroid and Xenobiotic Receptorを介したBisphenol Aの作用

    三木康宏, 長崎修治, 小野克彦, 赤平純一, 鈴木貴, 笹野公伸

    日本内分泌学会雑誌　83　(2)　467-467　2007/09

  318. 大腸癌におけるSteroid sulfataseとEstrogen sulfotransferaseの発現意義

    佐藤龍一郎, 鈴木貴, 三木康宏, 三浦康, 片寄友, 海野倫明, 笹野公伸

    日本内分泌学会雑誌　83　(2)　465-465　2007/09

  319. 乳癌における17beta-hydroxysteroid dehydrogenase type12の機能に関する検討

    長崎修治, 三木康宏, 赤平純一, 鈴木貴, 笹野公伸

    日本内分泌学会雑誌　83　(2)　465-465　2007/09

  320. ステロイドの微量測定 現状と今後の課題 子宮内膜癌細胞におけるエストロゲン合成・代謝酵素の発現 癌細胞のエストロゲン合成に対する間質細胞の影響

    宇都宮裕貴, 伊藤潔, 小林里香, 高橋尚美, 三木康宏, 鈴木貴, 本間誠次郎, 林慎一, 笹野公伸, 八重樫伸生

    日本内分泌学会雑誌　83　(2)　453-453　2007/09

  321. ステロイドの微量測定 現状と今後の課題 ヒト組織中ステロイドホルモン測定の臨床的病理的有用性 ホルモン依存性癌を例に

    鈴木貴, 三木康宏, 赤平純一, 笹野公伸

    日本内分泌学会雑誌　83　(2)　450-450　2007/09

    ISSN： 0029-0661

  322. In situ production of sex steroids in human breast carcinoma

    Takashi Suzuki, Yasuhiro Miki, Takuya Moriya, Jun-ichi Akahira, Hisashi Hirakawa, Noriaki Ohuchi, Hironobu Sasano

    MEDICAL MOLECULAR MORPHOLOGY　40　(3)　121-127　2007/09

    DOI： 10.1007/s00795-007-0365-8 　

    ISSN： 1860-1480

  323. 卵巣癌におけるEstrogen receptor-related receptorsの発現 Peer-reviewed

    赤平純一, 鈴木貴, 鈴木史彦, 三浦伊久美, 三木康宏, 長崎修治, 伊藤潔, 八重樫伸生, 笹野公伸

    日本癌学会総会記事　66回　507-507　2007/08

  324. 上皮性卵巣癌におけるCpG island methylator phenotypeの解析 Peer-reviewed

    鈴木史彦, 赤平純一, 三浦伊久美, 鈴木貴, 三木康宏, 伊藤潔, 八重樫伸生, 笹野公伸

    日本癌学会総会記事　66回　318-318　2007/08

  325. 骨芽細胞に対するSERMsの直接作用の比較

    三木康宏, 長崎修治, 赤平純一, 鈴木貴, 笹野公伸

    SERM: Selective Estrogen Receptor Modulator　(5)　74-75　2007/08

  326. Controversies of aromatase localization in human breast cancer-Stromal versus parenchymal cells

    Yasuhiro Miki, Takashi Suzuki, Hironobu Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　106　(1-5)　97-101　2007/08

    DOI： 10.1016/j.jsbmb.2007.05.007 　

    ISSN： 0960-0760

  327. Somatostatin receptor subtypes in human non-functioning neuroendocrine tumors and effects of somatostatin analogue SOM230 on cell proliferation in cell line NCI-H727 Peer-reviewed

    Katsuhiko Ono, Takashi Suzuki, Yasuhiro Miki, Yusuke Taniyama, Yasuhiro Nakamura, Yutaka Noda, Mika Watanabe, Hironobu Sasano

    ANTICANCER RESEARCH　27　(4B)　2231-2239　2007/07

    ISSN： 0250-7005

  328. [Intracrinology of human breast carcinoma and aromatase] Peer-reviewed

    Sasano Hironobu, Miki Yasuhiro

    Nippon Rinsho　65 Suppl 6　81-86　2007/06/28

  329. Early growth responsive gene 3 in human breast carcinoma: a regulator of estrogen-meditated invasion and a potent prognostic factor Peer-reviewed

    Takashi Suzuki, Akio Inoue, Yasuhiro Miki, Takuya Moriya, Jun-ichi Akahira, Takanori Ishida, Hisashi Hirakawa, Yuri Yamaguchi, Shin-ichi Hayashi, Hironobu Sasano

    ENDOCRINE-RELATED CANCER　14　(2)　279-292　2007/06

    DOI： 10.1677/ERC-06-0005 　

    ISSN： 1351-0088

  330. Nuclear cyclin B1 in human breast carcinoma as a potent prognostic factor Peer-reviewed

    Takashi Suzuki, Tomohiro Urano, Yasuhiro Miki, Takuya Moriya, Jun-ichi Akahira, Takanori Ishida, Kuniko Horie, Satoshi Inoue, Hironobu Sasano

    CANCER SCIENCE　98　(5)　644-651　2007/05

    DOI： 10.1111/j.1349-7006.2007.00444.x 　

    ISSN： 1347-9032

  331. ヒト上皮性卵巣癌におけるEstrogen receptor-related receptor(ERR)βの発現と臨床病理学的因子との関連について

    赤平純一, 鈴木貴, 鈴木史彦, 三浦伊久美, 三木康宏, 長崎修治, 鴨川由紀子, 伊藤潔, 森谷卓也, 林慎一, 八重樫伸生, 笹野公伸

    日本内分泌学会雑誌　83　(1)　148-148　2007/04

  332. ヒト骨芽細胞における内分泌撹乱物質Bisphenol Aの細胞増殖作用

    三木康宏, 長崎修治, 赤平純一, 鈴木貴, 笹野公伸

    日本内分泌学会雑誌　83　(1)　120-120　2007/04

  333. ヒト乳癌におけるEGR3(early growth response 3)の発現意義

    鈴木貴, 三木康宏, 森谷卓也, 赤平純一, 石田孝宣, 山口ゆり, 林慎一, 笹野公伸

    日本内分泌学会雑誌　83　(1)　117-117　2007/04

    Publisher:

    ISSN： 0029-0661

    eISSN： 2186-506X

  334. Effects of aromatase inhibitors on human osteoblast and osteoblast-like cells: A possible androgenic bone protective effects induced by exemestane Peer-reviewed

    Yasuhiro Miki, Takashi Suzuki, Masahito Hatori, Katsuhide Igarashi, Ken-ich Aisaki, Jun Kanno, Yasuhiro Nakamura, Miwa Uzuki, Takashi Sawai, Hironobu Sasano

    BONE　40　(4)　876-887　2007/04

    DOI： 10.1016/j.bone.2006.11.029 　

    ISSN： 8756-3282

  335. Aromatase localization in human breast cancer tissues: Possible interactions between intratumoral stromal and parenchymal cells Peer-reviewed

    Yasuhiro Miki, Takashi Suzuki, Chika Tazawa, Yuri Yamaguchi, Kunio Kitada, Seijiro Honma, Takuya Moriya, Hisashi Hirakawa, Dean B. Evans, Shin-ichi Hayashi, Noriaki Ohuchi, Hironobu Sasano

    CANCER RESEARCH　67　(8)　3945-3954　2007/04

    DOI： 10.1158/0008-5472.CAN-06-3105 　

    ISSN： 0008-5472

  336. 5 alpha-reductase type 1 and aromatase in breast carcinoma as regulators of in situ androgen production Peer-reviewed

    Takashi Suzuki, Yasuhiro Miki, Takuya Moriya, Jun-ichi Akahira, Takanori Ishida, Hisashi Hirakawa, Yuri Yamaguchi, Shin-ichi Hayashi, Hironobu Sasano

    INTERNATIONAL JOURNAL OF CANCER　120　(2)　285-291　2007/01

    DOI： 10.1002/ijc.22317 　

    ISSN： 0020-7136

  337. Expression profiles of sex steroid receptors in desmoid tumors Peer-reviewed

    Masato Ishizuka, Masahito Hatori, Osamu Dohi, Takashi Suzuki, Yasuhiro Miki, Chika Tazawa, Hironobu Sasano, Shoichi Kokubun

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　210　(3)　189-198　2006/11

    DOI： 10.1620/tjem.210.189 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  338. Immunolocalization of liver receptor homologue-1 (LRH-1) in human breast carcinoma: Possible regulator of in situ steroidogenesis Peer-reviewed

    Yasuhiro Miki, Colin D. Clyne, Takashi Suzuki, Takuya Moriya, Rie Shibuya, Yasuhiro Nakamura, Takanori Ishida, Nami Yabuki, Kunio Kitada, Shin-ichi Hayashi, Hironobu Sasano

    CANCER LETTERS　244　(1)　24-33　2006/11

    DOI： 10.1016/j.canlet.2005.11.038 　

    ISSN： 0304-3835

  339. Systemic distribution and tissue localizations of human 17beta-hydroxysteroid dehydrogenase type 12 Peer-reviewed

    N Sakurai, Y Miki, T Suzuki, K Watanabe, T Narita, K Ando, TMC Yung, D Aoki, H Sasano, H Handa

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　99　(4-5)　174-181　2006/06

    DOI： 10.1016/j.jsbmb.2006.01.010 　

    ISSN： 0960-0760

  340. 内分泌攪乱物質:Genistein,Bisphenol A,Diethylstilbestrolのヒト骨芽細胞に対する影響 Peer-reviewed

    三木康宏, 鈴木貴, 五十嵐勝秀, 菅野純, 笹野公伸

    日本内分泌学会雑誌　82　(1)　175-175　2006/04

  341. Peroxisome proliferator-activated receptor gamma in human breast carcinoma: a modulator of estrogenic actions Peer-reviewed

    T Suzuki, S Hayashi, Y Miki, Y Nakamura, T Moriya, A Sugawara, T Ishida, N Ohuchi, H Sasano

    ENDOCRINE-RELATED CANCER　13　(1)　233-250　2006/03

    DOI： 10.1677/erc.1.01075 　

    ISSN： 1351-0088

  342. Expression of the steroid and xenobiotic receptor and its possible target gene, organic anion transporting polypeptide-A, in human breast carcinoma Peer-reviewed

    Y Miki, T Suzuki, K Kitada, N Yabuki, R Shibuya, T Moriya, T Ishida, N Ohuchi, B Blumberg, H Sasano

    CANCER RESEARCH　66　(1)　535-542　2006/01

    DOI： 10.1158/0008-5472.CAN-05-1070 　

    ISSN： 0008-5472

  343. Sex steroid-producing enzymes in human breast cancer. (Endocr Relat Cancer)

    Suzuki Takashi, Miki Yasuhiro, Nakamura Yasuhiro, Moriya Takuya, Ito Kiyoshi, Ohuchi Noriaki, Sasano Hironobu

    Endocr Relat Cancer　12　(4)　701-720　2005/12

    DOI： 10.1677/erc.1.00834 　

  344. 内分泌撹乱とステロイド代謝-特にヒト組織における検索- Peer-reviewed

    三木康宏, 鈴木貴, 笹野公伸

    生体制御システムと内分泌撹乱　47-56　2005/09

  345. ヒト骨芽細胞におけるアロマターゼ阻害剤の影響 Peer-reviewed

    三木康宏, 鈴木貴, 五十嵐勝秀, 菅野純, 笹野公伸

    日本内分泌学会雑誌　81　(2)　451-451　2005/09

  346. Progesterone receptor in non-small cell lung cancer - A potent prognostic factor and possible target for endocrine therapy Peer-reviewed

    H Ishibashi, T Suzuki, S Suzuki, H Niikawa, LY Lu, Y Miki, T Moriya, S Hayashi, M Handa, T Kondo, H Sasano

    CANCER RESEARCH　65　(14)　6450-6458　2005/07

    DOI： 10.1158/0008-5472.CAN-04-3087 　

    ISSN： 0008-5472

  347. Analysis of gene expression induced by diethylstilbestrol (DES) in human primitive Mullerian duct cells using microarray Peer-reviewed

    Y Miki, T Suzuki, C Tazawa, M Ishizuka, S Semba, Gorai, I, H Sasano

    CANCER LETTERS　220　(2)　197-210　2005/04

    DOI： 10.1016/j.canlet.2004.07.024 　

    ISSN： 0304-3835

  348. Steroid and xenobiotic receptor (SXR), cytochrome P450 3A4 and multidrug resistance gene 1 in human adult and fetal tissues Peer-reviewed

    Y Miki, T Suzuki, C Tazawa, B Blumberg, H Sasano

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　231　(1-2)　75-85　2005/02

    DOI： 10.1016/j.mce.2004.12.005 　

    ISSN： 0303-7207

  349. Interactions between prostaglandin E-2, liver receptor homologue-1, and aromatase in breast cancer Peer-reviewed

    J Zhou, T Suzuki, A Kovacic, R Saito, Y Miki, T Ishida, T Moriya, ER Simpson, H Sasano, CD Clyne

    CANCER RESEARCH　65　(2)　657-663　2005/01

    ISSN： 0008-5472

  350. Analysis for localization of steroid sulfatase in human tissues Peer-reviewed

    T Suzuki, Y Miki, T Fukuda, T Nakata, T Moriya, H Sasano

    PHASE II CONJUGATION ENZYMES AND TRANSPORT SYSTEMS　400　303-316　2005

    DOI： 10.1016/S0076-6879(05)00018-2 　

    ISSN： 0076-6879

  351. Nur-related factor 1 and nerve growth factor-induced clone B in human adrenal cortex and its disorders Peer-reviewed

    LY Lu, T Suzuki, Y Yoshikawa, O Murakami, Y Miki, T Moriya, MH Bassett, WE Rainey, Y Hayashi, H Sasano

    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM　89　(8)　4113-4118　2004/08

    DOI： 10.1210/jc.2004-0069 　

    ISSN： 0021-972X

  352. Estrogen-related receptor a in human breast carcinoma as a potent prognostic factor Peer-reviewed

    T Suzuki, Y Miki, T Moriya, N Shimada, T Ishida, H Hirakawa, N Ohuchi, H Sasano

    CANCER RESEARCH　64　(13)　4670-4676　2004/07

    DOI： 10.1158/0008-5472.CAN-04-0250 　

    ISSN： 0008-5472

  353. ヒト副腎皮質腫瘍におけるNurr1及びNGFI-Bの発現 Peer-reviewed

    鈴木貴, 三木康宏, 森谷卓也, 笹野公伸

    日本病理学会会誌　93　(1)　224-224　2004/05

  354. ヒト副腎組織におけるNGFI-Bファミリーの発現 Peer-reviewed

    吉川陽介, 鈴木貴, 三木康宏, 村上治, 笹野公伸

    日本内分泌学会雑誌　80　(1)　112-112　2004/04

  355. ステロイドホルモン研究の最近の進歩 エストロゲン作用調節に関与する核内受容体の乳癌組織での発現 Peer-reviewed

    鈴木貴, 三木康宏, 笹野公伸

    日本内分泌学会雑誌　80　(1)　56-56　2004/04

  356. Estrogen receptors in atherosclerotic human aorta: inhibition of human vascular smooth muscle cell proliferation by estrogens Peer-reviewed

    Y Nakamura, T Suzuki, Y Miki, C Tazawa, K Senzaki, T Moriya, H Saito, T Ishibashi, S Takahashi, S Yamada, H Sasano

    MOLECULAR AND CELLULAR ENDOCRINOLOGY　219　(1-2)　17-26　2004/04

    DOI： 10.1016/j.mce.2004.02.013 　

    ISSN： 0303-7207

  357. Sex steroid receptors in rheumatoid arthritis Peer-reviewed

    M Ishizuka, M Hatori, T Suzuki, Y Miki, AD Darnel, C Tazawa, T Sawai, M Uzuki, Y Tanaka, S Kokubun, H Sasano

    CLINICAL SCIENCE　106　(3)　293-300　2004/03

    DOI： 10.1042/CS20030317 　

    ISSN： 0143-5221

  358. Aromatase localization in human breast cancer with development of new antibodies Peer-reviewed

    H Sasano, DP Edwards, TJ Anderson, SG Silverberg, DB Evans, RJ Santen, P Ramage, Y Miki, T Suzuki, ER Simpson, AS Bhatnagar, WR Miller

    ADVANCES IN ENDOCRINE THERAPY OF BREAST CANCER, PROCEEDINGS　277-286　2004

  359. Steroid sulfatase and estrogen sulfotransferase in the atherosclerotic human aorta Peer-reviewed

    Y Nakamura, Y Miki, T Suzuki, T Nakata, AD Darnel, T Moriya, C Tazawa, H Saito, T Ishibashi, S Takahashi, S Yamada, H Sasano

    AMERICAN JOURNAL OF PATHOLOGY　163　(4)　1329-1339　2003/10

    ISSN： 0002-9440

  360. 乳癌におけるliver receptor homologue-1(LRH-1)の発現 Peer-reviewed

    鈴木貴, 斉藤涼子, 三木康宏, 森谷卓也, 笹野公伸

    日本内分泌学会雑誌　79　(2)　420-420　2003/09

  361. Steroid sulfatase and estrogen sulfotransferase in normal human tissue and breast carcinoma Peer-reviewed

    T Suzuki, Y Miki, T Nakata, Y Shiotsu, S Akinaga, K Inoue, T Ishida, M Kimura, T Moriya, H Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　86　(3-5)　449-454　2003/09

    DOI： 10.1016/S0960-0760(03)00356-X 　

    ISSN： 0960-0760

  362. The possible roles of mineralocorticoid receptor and 11 beta-hydroxy steroid dehydrogenase type 2 in cardiac fibrosis in the spontaneously hypertensive rat Peer-reviewed

    A Konishi, C Tazawa, Y Miki, AD Darnel, T Suzuki, Y Ohta, T Suzuki, K Tabayashi, H Sasano

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　85　(2-5)　439-442　2003/06

    DOI： 10.1016/S0960-0760(03)00198-5 　

    ISSN： 0960-0760

  363. Estrogen sulfotransferase and steroid sulfatase in human breast carcinoma Peer-reviewed

    T Suzuki, T Nakata, Y Miki, C Kaneko, T Moriya, T Ishida, S Akinaga, H Hirakawa, M Kimura, H Sasano

    CANCER RESEARCH　63　(11)　2762-2770　2003/06

    ISSN： 0008-5472

  364. ヒト成人及び胎児組織におけるSXR,CYP3A及びMDR1の発現 Peer-reviewed

    三木康宏, 鈴木貴, 田澤智香, BlumbergBruce, 笹野公伸

    日本内分泌学会雑誌　79　(1)　148-148　2003/04

  365. Laser capture microscopy Peer-reviewed

    鈴木貴, 田澤智香, 三木康宏, 森谷卓也, 笹野公伸

    日本内分泌学会雑誌　79　(1)　95-95　2003/04

  366. Systemic distribution of steroid sulfatase and estrogen sulfotransferase in human adult and fetal tissues Peer-reviewed

    Y Miki, T Nakata, T Suzuki, AD Darnel, T Moriya, C Kaneko, K Hidaka, Y Shiotsu, H Kusaka, H Sasano

    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM　87　(12)　5760-5768　2002/12

    DOI： 10.1210/jc.2002-020670 　

    ISSN： 0021-972X

    eISSN： 1945-7197

  367. ホルモン依存性癌の最近の進歩 ヒト乳癌におけるIntracrinologyの新たなる展開 腫瘍内エストロゲンの活性化と不活性化 Peer-reviewed

    笹野公伸, 鈴木貴, 森谷卓也, 三木康宏, 中田泰介

    日本癌学会61回総会記事　24-24　2002/10

  368. Acute phase response in toxicity studies II. Findings in beagle dogs injected with endotoxin or subjected to surgical operation Peer-reviewed

    T. Hoshiya, D. Watanabe, T. Matsuoka, K. Horiguchi, Y. Miki, H. Mizuguchi, T. Ishii, N. Nomura, Y. Nagashima, A. Okaniwa

    Journal of Toxicological Sciences　26　(2)　103-109　2001

    DOI： 10.2131/jts.26.103 　

    ISSN： 0388-1350

Show all ︎Show first 5

Misc. 195

  1. Abstract 5484: Spatial transcriptomic profiling reveals characteristic gene expression patterns of cancer-associated fibroblasts in lung adenocarcinoma with interstitial pneumonia

    Chihiro Inoue, Yasuhiro Miki, Yayoi Aoyama, Saori Shibata, Yoshinori Okada, Takashi Suzuki

    Cancer Research　2024/03/22

    DOI： 10.1158/1538-7445.am2024-5484 　

    ISSN： 1538-7445

    More details Close

    <jats:title>Abstract</jats:title> <jats:p>Background: Interstitial pneumonia (IP) is a risk factor for lung cancer (LC). LC, a significant contributor to mortality, shows improved prognosis with molecular targeted drugs. However, LC combined with IP (LC with IP) has a poor prognosis because of acute exacerbations. Therefore, developing effective drugs for LC with IP is necessary. Cancer-associated fibroblasts (CAFs), influencing cancer progression through interactions with the microenvironment are anticipated as a new therapeutic target. Therefore, we hypothesized that CAFs in LC with IP, having undergone repeated destruction and regeneration, exhibit distinct characteristics from those in LC without IP and exert varied influences on carcinogenesis and cancer progression.</jats:p> <jats:p>Methods: Six cases of lung adenocarcinoma, the predominant type of LC, were enrolled in this study. Spatial profiling was performed on FFPE specimens from three LC with IP and three LC without IP cases, using GeoMx whole-transcriptome atlas assay. Immunostaining for α-SMA, a major CAFs-marker, and pan-cytokeratin (CK), an epithelial marker, were employed to identify regions of interest (ROIs). In LC with IP, two ROIs were designated for cancerous and non-cancerous areas, respectively, whereas in LC without IP, two ROIs were selected for cancerous areas. Each ROI was segmented for epithelium and cancer (CK+/α-SMA -) and α-SMA-positive stroma (CK-/α-SMA +) areas of illumination. NGS data were analyzed using the edgeR to compare mRNA expressions in cancerous (LC-IP) and non-cancerous (NC) areas of LC with IP. Additionally, comparisons were made between LC-IP and cancerous areas of LC without IP (LC-nonIP) for mRNA expression.</jats:p> <jats:p>Results: Analysis of α-SMA-positive stroma revealed 86 differentially expressed genes (DEGs) in LC-IP vs. NC. Notably, COTL1 and NFKB2 increased, whereas TNC and MMP7 were decreased in LC-IP α-SMA-positive stroma. Pathway Enrichment analysis indicated activation of the response to cytokines. Between LC-IP and LC-nonIP, 151 DEGs were detected. CXCL8 and NFKB2 were elevated, whereas ACAN and ELN decreased in LC-IP α-SMA-positive stroma. KEGG pathway analysis demonstrated inactivated ECM-receptor interactions. Analysis of CK-positive regions showed 710 DEGs in LC-IP vs. NC, and KEGG pathway analysis indicated inactivated ECM-receptor interaction. In LC-IP vs. LC-nonIP, 321 DEGs were detected, with KEGG pathway analysis suggesting inactivation of ECM-receptor interaction and activation of a chemokine signaling pathway and cytokine-cytokine receptor interaction.</jats:p> <jats:p>Conclusion: These findings suggest that inflammatory factors may play a more critical role than extracellular matrix in the interaction between α-SMA-positive stroma and CK-positive cancer cells in LC with IP. Future plans include conducting in vitro experiments, as well as immunohistological studies.</jats:p> <jats:p>Citation Format: Chihiro Inoue, Yasuhiro Miki, Yayoi Aoyama, Saori Shibata, Yoshinori Okada, Takashi Suzuki. Spatial transcriptomic profiling reveals characteristic gene expression patterns of cancer-associated fibroblasts in lung adenocarcinoma with interstitial pneumonia [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 5484.</jats:p>

  2. 間質性肺炎における浸潤性粘液性腺癌発生機序の検討

    小山 涼子, 村上 圭吾, 村上 一宏, 三木 康宏, 井上 千裕, 田畑 俊治, 岡田 克典, 鈴木 貴, 鈴木 博義, 笹野 公伸

    日本病理学会会誌　112　(1)　330-330　2023/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  3. 肺腺癌の癌細胞と間質におけるvasohibin-2発現に関する検討

    井上 千裕, 熱海 南帆, 三木 康宏, 小山 涼子, 岡田 克典, 鈴木 貴

    日本病理学会会誌　112　(1)　329-329　2023/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  4. Immunohistochemical analysis of cellular senescence markers and target molecule of nintedanib in IPF

    井上千裕, 清水弘明, 三木康宏, 齊藤涼子, 岡田克典, 笹野公伸

    日本病理学会会誌　111　(1)　2022

    ISSN： 0300-9181

  5. 令和パンデミックの今、大正パンデミックを計量書誌学的に考察する

    三木 康宏, 中鉢 奈津子, 川内 淳史, 児玉 栄一, 伊藤 潔

    日本生殖内分泌学会雑誌　26　36-38　2021/08

    Publisher: 日本生殖内分泌学会

    ISSN： 1348-8031

  6. 令和パンデミックの今、大正パンデミックを計量書誌学的に考察する

    三木 康宏, 中鉢 奈津子, 川内 淳史, 児玉 栄一, 伊藤 潔

    日本内分泌学会雑誌　96　(4)　1065-1065　2021/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  7. 子宮内膜症及び腺筋症におけるエストロゲン合成・代謝酵素の発現

    近藤 悠人, 齊藤 涼子, 三木 康宏, Poutanen Matti, Lammintausta Risto, 八重樫 伸生, 伊藤 潔, 笹野 公伸

    日本内分泌学会雑誌　96　(4)　1072-1072　2021/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  8. 特発性肺線維症合併肺腺癌における癌関連線維芽細胞マーカータンパク発現に関する検討

    前田さき, 井上千裕, 井上千裕, 三木康宏, 齊藤涼子, 齊藤涼子, 岡田克典, 笹野公伸, 笹野公伸

    日本癌学会学術総会抄録集(Web)　80th　2021

  9. 肺腺癌の癌関連線維芽細胞におけるp16,p21発現の影響

    井上千裕, 井上千裕, 三木康宏, 齊藤涼子, 齊藤涼子, 岡田克典, 笹野公伸, 笹野公伸

    日本癌学会学術総会抄録集(Web)　80th　2021

  10. 災害とワンヘルス：ヒトと動物との関係

    三木 康宏

    ワンヘルス サイエンティスト　1　(3)　2020/12

  11. ステロイドホルモン：量と作用を可視化する

    鈴木貴, 岩渕英里奈, 三木康宏, 高木清司, 笹野公伸

    組織細胞化学 2019　143-151　2019/07

  12. ホルモン依存性癌の新展開 ホルモン依存性癌におけるホルモンシグナルの可視化

    三木 康宏, 岩渕 英里奈, 鈴木 貴, 笹野 公伸, 伊藤 潔

    日本内分泌学会雑誌　95　(1)　196-196　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  13. 子宮内膜癌におけるステロイドホルモンとKrueppel-like factor 5の発現

    三木 康宏, 吉田 伶奈, 高木 清司, 鈴木 貴, 伊藤 潔

    日本内分泌学会雑誌　95　(1)　445-445　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  14. 乳癌におけるKLK12の発現意義

    吉村 彩乃, 塚本 若菜, 佐藤 和, 高木 清司, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　445-445　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  15. ER陽性浸潤性乳癌におけるCYC1の発現意義

    佐藤 和, 高木 清司, 三木 康宏, 吉村 彩乃, 原 瑞季, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　473-473　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  16. 乳癌における5α-reductase type 3の発現意義

    高木 清司, 佐藤 和, 井上 直紀, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　473-473　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  17. 乳癌におけるCLEC2Dの発現意義

    栗原 唯, 高木 清司, 保田 伊織, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　488-488　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  18. 乳癌組織随伴マクロファージにおけるアンドロゲン誘導性液性因子の解析

    山口 美桜, 高木 清司, 佐藤 正康, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本内分泌学会雑誌　95　(1)　488-488　2019/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  19. ヒト肺癌における腫瘍内Glucocorticoid産生が腫瘍内免疫微小環境に与える影響

    齊藤涼子, 三木康宏, 井上千裕, 七宮蓮, 阿部二郎, 佐藤郁郎, 笹野公伸

    日本病理学会会誌　108　(1)　2019

    ISSN： 0300-9181

  20. OsimertinibのEGFR非依存的な抗腫瘍効果に関する検討

    七宮蓮, 七宮蓮, 齊藤涼子, 小林雅行, 三木康宏, 三木康宏, 井上千裕, 笹野公伸

    日本病理学会会誌　108　(1)　2019

    ISSN： 0300-9181

  21. EGFR-independent tumor inhibitory effects of Osimertinib via EphB4-cell cycle pathway

    七宮蓮, 齊藤涼子, 三木康宏, 井上千裕, 笹野公伸

    日本癌学会学術総会抄録集(Web)　78th　2019

  22. 子宮内膜癌におけるDehydroepiandrosteroneの組織中濃度とその意義

    三木 康宏, 吉田 伶奈, 高木 清司, 鈴木 貴, 伊藤 潔

    日本内分泌学会雑誌　94　(4)　1441-1441　2018/12

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  23. 乳癌におけるCytokeratin 19の発現局在の検討

    岩渕 英里奈, 三木 康宏, 金井 綾子, 高木 清司, 鈴木 貴, 石田 孝宣, 笹野 公伸

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集　59回　79-79　2018/09

    Publisher: 日本組織細胞化学会

  24. エストロゲン (特集 産婦人科関連ホルモンの基礎を学びなおす)

    三木 康宏, 伊藤 潔

    産科と婦人科　85　(7)　741-749　2018/07

    Publisher: 診断と治療社

    ISSN： 0386-9792

  25. トリプルネガティブ乳癌におけるグルココルチコイドの生物学的作用

    金井 綾子, McNamara Keely May, Fouzia Guestini, 岩渕 英里奈, 小野寺 好明, 三木 康宏, 宮下 穣, 山口 倫, 大井 恭代, 相良 安昭, 石田 孝宣, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　26回　580-580　2018/05

    Publisher: (一社)日本乳癌学会

  26. 肺腺癌における癌関連線維芽細胞由来サイトカインによるPD-L1発現の誘導

    井上 千裕, 三木 康宏, 齊藤 涼子, 端 秀子, 渡辺 みか, 佐藤 郁郎, 岡田 克典, 笹野 公伸

    日本病理学会会誌　107　(1)　415-415　2018/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  27. HER2陽性乳癌におけるCytokeratin 19およびHER2の相互作用の検討

    岩渕 英里奈, 三木 康宏, 金井 綾子, 石田 孝宣, 笹野 公伸

    日本病理学会会誌　107　(1)　426-427　2018/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  28. 肺リンパ脈管筋腫症におけるヒト上皮成長因子受容体ファミリーの発現に関する検討

    小林 数真, 齊藤 涼子, 三木 康宏, 渡辺 みか, 岡田 克典, 笹野 公伸

    日本病理学会会誌　107　(1)　474-474　2018/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  29. 肺腺癌組織における癌関連線維芽細胞マーカーの比較検討

    玉槻 大輔, 井上 千裕, 三木 康宏, 齊藤 涼子, 岡田 克典, 笹野 公伸

    日本病理学会会誌　107　(1)　522-522　2018/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  30. エストロゲン

    三木康宏, 伊藤潔

    産科と婦人科　85　(7)　741-749　2018

  31. トラスツズマブ奏功性におけるHER2と癌胎児性抗原関連細胞接着分子6の相互作用の意義の検討

    岩渕 英里奈, 三木 康宏, 櫻井 美奈子, 宮下 穣, 金井 綾子, 石田 孝宣, 笹野 公伸

    日本癌学会総会記事　76回　P-2182　2017/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  32. 乳癌における遠隔転移に関連するOLFM4、LY6、S100A7の免疫局在

    真山 晃史, 鈴木 博義, 高木 清, 小野寺 好明, 渡邊 隆紀, 三木 康宏, 坂元 和宏, 吉田 龍一, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　76回　P-2200　2017/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  33. 乳癌組織における腫瘍随伴マクロファージに対するアンドロゲンの影響の解析

    佐藤 正康, 高木 清司, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　76回　P-2201　2017/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  34. S100Pとezrinはトリプルネガティブ乳癌の経内皮浸潤を促進する

    菊地 杏子, McNamara Keely M, 三木 康宏, 岩渕 英里奈, 宮下 穣, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　76回　J-2052　2017/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  35. 癌関連線維芽細胞の肺腺癌細胞でのPD-L1発現に対する影響

    井上 千裕, 三木 康宏, 齊藤 涼子, 端 秀子, 佐藤 郁郎, 前門戸 任, 岡田 克典, 岡部 尚文, 笹野 公伸

    肺癌　57　(5)　473-473　2017/09

    Publisher: (NPO)日本肺癌学会

    ISSN： 0386-9628

    eISSN： 1348-9992

  36. トリプルネガティブ乳癌におけるS100Pとezrinの発現意義および相互作用に関する検討

    菊地 杏子, McNamara Keely M, 三木 康宏, 宮下 穣, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　25回　353-353　2017/07

    Publisher: (一社)日本乳癌学会

  37. trastuzumab奏功性におけるcarcinoembryonic antigen-related cell adhesion 6の発現意義

    岩渕 英里奈, 三木 康宏, 櫻井 美奈子, 宮下 穣, 金井 綾子, 石田 孝宣, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　25回　413-413　2017/07

    Publisher: (一社)日本乳癌学会

  38. The 17 beta-Hydroxysteroid Dehydrogenase Type 2 Expression Induced by the Androgen Signal in Endometrial Cancer.

    Chiaki Hashimoto, Yasuhiro Miki, Sota Tanaka, Kiyoshi Takagi, Misaki Fue, Zhulanqiqige Doe, Bin Li, Nobuo Yaegashi, Takashi Suzuki, Kiyoshi Ito

    REPRODUCTIVE SCIENCES　24　202A-202A　2017/03

    ISSN： 1933-7191

    eISSN： 1933-7205

  39. MMP-1 as a Marker of Adverse Clinical Outcome of Pulmonary Adenocarcinoma Patients: The Correlation Between MMP-1 Overexpression and Clinicopathological Factors and EGFR-TKI Resistance

    Ryoko Saito, Yasuhiro Miki, Naoya Ishida, Shuko Hata, Hironobu Sasano

    MODERN PATHOLOGY　30　493A-493A　2017/02

    ISSN： 0893-3952

    eISSN： 1530-0285

  40. MMP-1 as a Marker of Adverse Clinical Outcome of Pulmonary Adenocarcinoma Patients: The Correlation Between MMP-1 Overexpression and Clinicopathological Factors and EGFR-TKI Resistance

    Ryoko Saito, Yasuhiro Miki, Naoya Ishida, Shuko Hata, Hironobu Sasano

    LABORATORY INVESTIGATION　97　493A-493A　2017/02

    ISSN： 0023-6837

    eISSN： 1530-0307

  41. 非浸潤性乳管癌におけるCYC1の発現意義

    佐藤 和, 高木 清司, 三木 康宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　75回　P-2158　2016/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  42. 浸潤性小葉癌と浸潤性乳管癌における微小環境 Cancer-associated fibroblastsと血管新生に関する検討

    中川 紗紀, 三木 康宏, 宮下 穣, 鈴木 昭彦, 多田 寛, 渡部 剛, 原田 成美, 佐藤 章子, 佐藤 千穂, 高木 まゆ, 玉城 研太朗, 大井 恭代, 平川 久, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　24回　471-471　2016/06

    Publisher: (一社)日本乳癌学会

  43. EGFR-TKI耐性獲得肺腺癌におけるNupr1の発現意義に関する研究

    杉浦 弘樹, 三木 康宏, 秦 秀子, 齊藤 涼子, 佐藤 郁郎, 岡部 尚文, 笹野 公伸

    日本病理学会会誌　105　(1)　332-332　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  44. 27-ヒドロキシコレステロール合成酵素であるCYP27A1の乳房腫瘍微小環境における役割(The roles of 27-hydroxycholesterol synthesizing enzyme, CYP27A1 in breast tumor micoenvironment)

    櫻井 美奈子, 三木 康宏, 高木 清司, 鈴木 貴, 石田 孝宜, 大内 憲明, 笹野 公伸

    日本病理学会会誌　105　(1)　373-373　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  45. リンパ脈管筋腫症におけるEGFRの発現意義

    小林 数真, 齊藤 涼子, 三木 康宏, 井上 千裕, 小野 克彦, 端 秀子, 渡辺 みか, 岡田 克典, 笹野 公伸

    日本病理学会会誌　105　(1)　391-391　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  46. 超解像顕微鏡を用いたエストロゲン受容体発現局在の解析

    岩渕 英里奈, 三木 康宏, 小野 克彦, 小野寺 好明, 笹野 公伸

    日本病理学会会誌　105　(1)　466-466　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  47. EGFR-TKI耐性非小細胞肺癌におけるCYP2E1の発現意義

    三木 康宏, 端 秀子, 吉田 奈緒子, 齊藤 涼子, 佐藤 郁郎, 岡部 尚文, 笹野 公伸

    日本病理学会会誌　105　(1)　532-532　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  48. 非小細胞性肺癌におけるNucleobindin 2の発現意義

    山中 大空, 三木 康宏, 端 秀子, 杉浦 弘樹, 齊藤 涼子, サマネ・ヤズダニ, 佐藤 郁郎, 岡部 尚文, 笹野 公伸

    日本病理学会会誌　105　(1)　533-533　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  49. 肺腺癌におけるMMP-1とEGFR-TKI耐性との関わり

    石田 直也, 齊藤 涼子, 三木 康宏, 端 秀子, 杉浦 弘樹, 小林 雅行, 井上 千裕, 渡辺 みか, 岡田 克典, 笹野 公伸

    日本病理学会会誌　105　(1)　586-586　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  50. 乳癌における癌関連線維芽細胞によるアンドロゲン合成酵素の発現誘導

    菊地 杏子, McNamara Keely, 三木 康宏, 櫻井 美奈子, 笹野 公伸

    日本病理学会会誌　105　(1)　592-592　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  51. 剖検における多重癌症例の病理組織学的検討

    東野 江里, 柴原 裕紀子, 高橋 裕明, 楊 陽, 三木 康宏, 鈴木 高祐, 笹野 公伸

    日本病理学会会誌　105　(1)　605-605　2016/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  52. Malignant Tumors Associated with Breast Cancer: Analysis of 164,211 Autopsy Cases in Japan

    Yukiko Shibahara, Yumi Sugawara, Yasuhiro Miki, Yasuhiro Nakamura, Ichiro Tsuji, Hironobu Sasano

    MODERN PATHOLOGY　29　10A-10A　2016/02

    ISSN： 0893-3952

    eISSN： 1530-0285

  53. Malignant Tumors Associated with Breast Cancer: Analysis of 164,211 Autopsy Cases in Japan

    Yukiko Shibahara, Yumi Sugawara, Yasuhiro Miki, Yasuhiro Nakamura, Ichiro Tsuji, Hironobu Sasano

    LABORATORY INVESTIGATION　96　10A-10A　2016/02

    ISSN： 0023-6837

    eISSN： 1530-0307

  54. 培養細胞をみる ー免疫細胞化学から電子顕微鏡までー

    三木康宏, 岩渕英里奈, 笹野公伸

    組織細胞化学2016　109-121　2016

    Publisher: 日本組織細胞化学会

  55. 乳癌におけるmiR-1の発現意義と、予後因子としての可能性

    峯村 洋行, 高木 清司, 三木 康宏, 柴原 裕紀子, 中川 紗紀, 江幡 明子, 渡辺 みか, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　74回　P-2231　2015/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  56. 乳癌におけるHER2とcarcinoembryonic antigen-related cell adhesion 6の相互作用の検討

    岩渕 英里奈, 三木 康宏, 櫻井 美奈子, 笹野 公伸

    日本癌学会総会記事　74回　P-2232　2015/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  57. 子宮内膜癌増殖におけるストレスホルモン-コルチゾールの関与

    三木 康宏, 笛 未崎, 高木 清司, 鈴木 貴, 笹野 公伸, 伊藤 潔

    日本癌学会総会記事　74回　P-2135　2015/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  58. EGFR-TKI耐性非小細胞肺癌におけるCYP2E1の発現意義

    端 秀子, 三木 康宏, 吉田 奈緒子, 齊藤 涼子, 佐藤 郁郎, 岡部 尚文, 笹野 公伸

    日本癌学会総会記事　74回　P-2213　2015/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  59. 浸潤性小葉癌と乳管癌におけるエストロゲン合成代謝酵素の比較およびエストロゲン応答遺伝子の発現について

    高木 まゆ, 三木 康宏, 宮下 穣, 端 秀子, 依田 智美, 鈴木 貴, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　74回　P-2249　2015/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  60. 癌関連脂肪細胞と乳癌細胞の相互作用によるリポカリン2の発現誘導とその意義

    櫻井 美奈子, 河原 由依, 三木 康宏, 高木 清司, 鈴木 貴, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　74回　E-1345　2015/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  61. 乳癌におけるCITED2の発現意義

    高木 清司, 峯村 洋行, 三木 康宏, 高橋 光, 中村 保宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　74回　P-2240　2015/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  62. 164,211例の剖検症例を用いた多重癌の疫学的検討 乳癌に合併する悪性腫瘍について

    柴原 裕紀子, 菅原 由美, 三木 康宏, 大内 憲明, 辻 一郎, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　23回　304-304　2015/07

    Publisher: (一社)日本乳癌学会

  63. 浸潤性小葉癌と浸潤性乳管癌におけるエストロゲン合成代謝酵素の比較

    高木 まゆ, 石田 孝宣, 三木 康宏, 宮下 穣, 雷 哲明, 平川 久, 笹野 公伸, 大内 憲明

    日本乳癌学会総会プログラム抄録集　23回　536-536　2015/07

    Publisher: (一社)日本乳癌学会

  64. 【呼吸器疾患とオートファジー】 肺癌とオートファジー

    三木 康宏, 仁平 開人, 齊藤 涼子, 笹野 公伸

    THE LUNG-perspectives　23　(2)　154-158　2015/05

    Publisher: (株)メディカルレビュー社

    ISSN： 0919-5742

  65. 乳癌微小環境における癌と脂肪細胞の相互作用に関与するLCN2の役割

    櫻井 美奈子, 河原 由依, 三木 康宏, 高木 清司, 鈴木 貴, 石田 孝宜, 大内 憲明, 笹野 公伸

    日本内分泌学会雑誌　91　(1)　373-373　2015/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  66. 乳腺・内分泌 浸潤性小葉癌と浸潤性乳管癌におけるエストロゲン合成代謝酵素の比較

    高木 まゆ, 石田 孝宣, 三木 康宏, 玉城 研太朗, 雷 哲明, 平川 久, 笹野 公伸, 大内 憲明

    日本外科学会定期学術集会抄録集　115回　OP-045　2015/04

    Publisher: (一社)日本外科学会

  67. HER2陽性乳癌におけるcarcinoembryonic antigen-related cell adhesion 6の発現意義

    岩渕 英里奈, 三木 康宏, 小野 克彦, 笹野 公伸

    日本病理学会会誌　104　(1)　455-455　2015/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  68. 乳癌の原発および転移巣における線維芽細胞について 免疫組織学的検討(Stromal fibroblasts in primary and metastatic breast cancer: An immunohistochemical study)

    柴原 裕紀子, 三木 康宏, 高橋 裕明, 中村 保宏, 鈴木 貴, 笹野 公伸

    日本病理学会会誌　104　(1)　318-318　2015/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  69. 肺癌とオートファジー

    三木康宏, 仁平開人, 齊藤涼子, 笹野公伸

    THE LUNG perspectives　23　(2)　42-46　2015

    Publisher: 株式会社メディカルレビュー社

  70. エストロゲンレセプターダイマーの発現局在の可視化とパターン解析

    岩渕 英里奈, 三木 康宏, 小野寺 好明, 小野 克彦, 鈴木 貴, 笹野 公伸

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集　55回　78-78　2014/09

    Publisher: 日本組織細胞化学会

  71. 乳癌におけるS100A7の脂肪由来因子による誘導とその発現意義(S100A7 in breast carcinoma invasion into adipose tissue)

    櫻井 美奈子, 三木 康宏, 高木 清司, 鈴木 貴, 笹野 公伸

    日本癌学会総会記事　73回　E-3016　2014/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  72. 子宮内膜癌におけるNUCB2の発現意義(NUCB2 in human endometrial carcinoma as a potent prognostic factor)

    高木 清司, 三木 康宏, 田中 創太, 八重樫 伸生, 笹野 公伸, 伊藤 潔, 鈴木 貴

    日本癌学会総会記事　73回　P-1362　2014/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  73. ステロイドホルモン合成酵素AKR1C3は11β-Prostaglandin F2αの産生に寄与し、乳癌の悪化に関わる(11β-Prostaglandin F2α produced by AKR1C3 stimulates FP-receptor and contributes to breast cancer progression)

    依田 智美, McNamara Keely M, 三木 康宏, 高木 清司, 鈴木 貴, 笹野 公伸

    日本癌学会総会記事　73回　P-3312　2014/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  74. 乳癌細胞におけるエストロゲン誘導性エストロゲンレセプターダイマーパターンの検出(Detection of estrogen-induced dimerization patterns of estrogen receptor in breast carcinoma cells)

    岩渕 英里奈, 三木 康宏, 小野寺 好明, 小野 克彦, 笹野 公伸

    日本癌学会総会記事　73回　P-2362　2014/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  75. 乳癌組織におけるTransforming acidic coiled-coil-containing protein 2(TACC2)の免疫局在(Transforming acidic coiled-coil-containing protein 2 (TACC2) immunolocalization in breast carcinoma)

    小野寺 好明, 高木 清司, 三木 康宏, 中村 保宏, 渡辺 みか, 石田 孝宣, 笹野 公伸, 井上 聡, 鈴木 貴

    日本癌学会総会記事　73回　P-3321　2014/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  76. Intratumoral Concentration of Stress Hormone, Cortisol in Endometrial Carcinoma

    Yasuhiro Miki, Kiyoshi Takagi, Zhulanqiqige Doe, Sota Tanaka, Takashi Suzuki, Hironobu Sasano, Kiyoshi Ito

    ENDOCRINE REVIEWS　35　(3)　2014/06

    ISSN： 0163-769X

    eISSN： 1945-7189

  77. Identification of Signaling Factors Involved in an Interaction Between Human Breast Carcinoma and Adipocytes

    Minako Sakurai, Yasuhiro Miki, Kiyoshi Takagi, Takashi Suzuki, Hironobu Sasano

    ENDOCRINE REVIEWS　35　(3)　2014/06

    ISSN： 0163-769X

    eISSN： 1945-7189

  78. オートファジー関連因子LC3AはEGFRチロシンキナーゼ阻害剤奏功性予測マーカーになり得る

    仁平 開人, 三木 康宏, 鳴海 創大, 飯田 慎也, 小野 克彦, 海老名 雅仁, 笹野 公伸

    日本分子腫瘍マーカー研究会誌　29　34-35　2014/04

    Publisher: 日本分子腫瘍マーカー研究会

    DOI： 10.11241/jsmtmr.29.34 　

  79. 乳癌におけるheterogeneous nuclear ribonucleoprotein Kの発現意義

    岩渕 英里奈, 三木 康宏, 増田 真理子, 柴原 裕紀子, 鈴木 貴, 笹野 公伸

    日本病理学会会誌　103　(1)　352-352　2014/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  80. mTOR阻害剤による薬剤性肺障害の剖検例を含む肺組織におけるmTOR活性の免疫組織学的解析

    齊藤 涼子, 柳内 充, 三木 康宏, 笠島 敦子, 藤島 史喜, 中村 保宏, 海老名 雅仁, 深澤 雄一郎, 渡辺 みか, 笹野 公伸

    日本病理学会会誌　103　(1)　339-339　2014/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  81. Progesterone Receptor and Its Synthesizing Enzymes in Pancreatic Neuroendocrine Tumor

    A. Kasajima, S. Yazdani, H. Ogata, S. J. A. Felizola, Y. Miki, Y. Nakamura, H. Sasano

    LABORATORY INVESTIGATION　94　154A-155A　2014/02

    ISSN： 0023-6837

    eISSN： 1530-0307

  82. Progesterone Receptor and Its Synthesizing Enzymes in Pancreatic Neuroendocrine Tumor

    A. Kasajima, S. Yazdani, H. Ogata, S. J. A. Felizola, Y. Miki, Y. Nakamura, H. Sasano

    MODERN PATHOLOGY　27　154A-155A　2014/02

    ISSN： 0893-3952

    eISSN： 1530-0285

  83. 肺癌における喫煙とエストロゲン

    三木康宏, 笹野公伸

    医学のあゆ　242　949-950　2014

  84. O-12 エストロゲンレセプターダイマーの発現局在の可視化とパターン解析(内分泌・生殖器,一般口演,第55回日本組織細胞化学会総会・学術集会 第11回日中合同組織細胞化学セミナー)

    岩渕 英里奈, 三木 康宏, 小野寺 好明, 小野 克彦, 鈴木 貴, 笹野 公伸

    日本組織細胞化学会総会プログラムおよび抄録集　(55)　78-78　2014

    Publisher: 日本組織細胞化学会

  85. 肺癌における喫煙とエストロゲン

    三木康宏, 笹野公伸

    医学のあゆみ　249　(13)　1326-1327　2014

    Publisher: 医歯薬出版株式会社

  86. Y Estrogenic pathways exist in TNBC and correlate with androgen receptor status

    K. M. McNamara, T. Yoda, A. M. Nurani, Y. Miki, T. Suzuki, N. Nemoto, M. Miyashita, K. Tamaki, T. Ishida, N. Ohuchi, H. Sasano

    CANCER RESEARCH　73　2013/12

    DOI： 10.1158/0008-5472.SABCS13-P5-05-10 　

    ISSN： 0008-5472

    eISSN： 1538-7445

  87. INTRATUMORAL ANDROGEN CONCENTRATIONS IN ENDOMETRIAL CARCINOMA TISSUES

    S. Tanaka, Y. Miki, T. Suzuki, D. Zhulanqiqige, K. Takagi, H. Utsunomiya, S. Nagase, H. Niikura, N. Yaegashi, K. Ito

    INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER　23　(8)　2013/10

    ISSN： 1048-891X

    eISSN： 1525-1438

  88. 糖尿病とがん 乳癌発症/進展における脂肪細胞とエストロゲン代謝(Diabetes and Cancer(Japan Diabetes Society) Breast cancer, estrogen and obesity)

    笹野 公伸, 櫻井 美奈子, 三木 康宏

    日本癌学会総会記事　72回　11-11　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  89. p62/SQSTM1は癌進展における重要なautophagy制御因子である(A Role for p62/SQSTM1 as a Crucial Mediator of Autophagy during Cancer Progression)

    仁平 開人, 三木 康宏, 小野 克彦, 鈴木 貴, 笹野 公伸

    日本癌学会総会記事　72回　120-120　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  90. RNA結合蛋白Musashi-1と協調的に乳癌進展に寄与するLin28Bの新規治療標的としての可能性(Lin28B is a new potential molecular target in breast cancer progression, and enhanced by RNA binding protein, Musashi)

    櫻井 美奈子, 三木 康宏, 柴原 裕紀子, 鈴木 貴, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　72回　162-162　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  91. 乳癌の転移リンパ節におけるER、PR、HER2、Ki-67発現と術前化学療法の関与について(The effect of preoperative chemotherapy on ER, PR, HER2 and Ki-67 expression in metastatic lymph node of breast cancer)

    内田 惠子, 柴原 裕紀子, 三木 康宏, 端 秀子, 岩渕 英里奈, 根本 紀子, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　72回　324-324　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  92. ヒト乳癌におけるNRF2発現の免疫組織化学的検討(Immunolocalization of NRF2 in human breast carcinoma)

    小野寺 好明, 本橋 ほづみ, 高木 清司, 三木 康宏, 柴原 裕紀子, 渡辺 みか, 石田 孝宣, 笹野 公伸, 山本 雅之, 鈴木 貴

    日本癌学会総会記事　72回　403-403　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  93. 乳癌細胞におけるエストロゲンレセプターダイマーの検出(In situ detection of estrogen receptor dimers in breast carcinoma cells)

    岩渕 英里奈, 三木 康宏, 小野寺 好明, 小野 克彦, 笹野 公伸

    日本癌学会総会記事　72回　404-404　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  94. Molecular Apocrine乳癌における17βHSD5のアンドロゲンによる発現調節(Androgenic Regulation of 17βHSD5 Expression in Molecular Apocrine Breast Cancer)

    依田 智美, McNamara Keely, 三木 康宏, 高木 清司, 鈴木 貴, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　72回　428-428　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  95. 乳癌におけるARHGAP15の発現意義(Immunolocalization of ARHGAP15 in human breast carcinoma)

    高木 清司, 三木 康宏, 小野寺 好明, 中村 保宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　72回　425-425　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  96. 非小細胞肺癌におけるGas6によるAxl分解の意義(Novel Roles of Axl cleavage by its ligand Gas6 in non-small cell lung carcinoma)

    飯田 慎也, 三木 康宏, 中村 保宏, 鈴木 貴, 岡部 尚文, 笹野 公伸

    日本癌学会総会記事　72回　461-461　2013/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  97. ヒト子宮内膜癌におけるCRH発現と予後の関連

    佐藤 菜保子, 高木 清司, 鈴木 貴, 三木 康宏, 割田 仁, 福土 審, 佐藤 冨美子, 八重樫 伸生, 伊藤 潔

    日本癌治療学会誌　48　(3)　1991-1991　2013/09

    Publisher: (一社)日本癌治療学会

    ISSN： 0021-4671

  98. 近接ライゲーションアッセイ法を用いた乳癌培養細胞におけるPLA法を用いたエストロゲンレセプターダイマーの検出

    岩渕 英里奈, 三木 康宏, 小野寺 好明, 小野 克彦, 笹野 公伸

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集　54回　(54)　88-88　2013/09

    Publisher: 日本組織細胞化学会

  99. オートファジー関連因子LC3AはEGFRチロシンキナーゼ阻害剤奏功性予測マーカーになり得る

    仁平 開人, 三木 康宏, 鳴海 創大, 飯田 慎也, 小野 克彦, 海老名 雅仁, 笹野 公伸

    日本分子腫瘍マーカー研究会プログラム・講演抄録　33回　60-61　2013/09

    Publisher: 日本分子腫瘍マーカー研究会

  100. 浸潤性小葉癌におけるアンドロゲン代謝酵素の発現とその意義の解明

    依田 智美, マクナマラ・キーリー, 三木 康宏, 高木 まゆ, 雷 哲明, 平川 久, 石田 孝宣, 鈴木 貴, 大内 憲明, 笹野 公伸

    日本内分泌学会雑誌　89　(2)　631-631　2013/09

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  101. 閉経後非浸潤性乳管癌に対するレトロゾール術前内分泌療法の検討

    江幡 明子, 石田 孝宣, 高木 清司, 三木 康宏, 平川 久, 角川 陽一郎, 天野 吾郎, 森 奈緒子, 中村 保宏, 渡辺 みか, 甘利 正和, 笹野 公伸, 鈴木 貴, 大内 憲明

    日本癌治療学会誌　48　(3)　1574-1574　2013/09

    Publisher: (一社)日本癌治療学会

    ISSN： 0021-4671

  102. 肺癌・乳癌におけるp62/SQSTM1の発現動態およびその意義

    仁平 開人, 三木 康宏, 鈴木 貴, 笹野 公伸

    日本病理学会会誌　102　(1)　294-294　2013/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  103. 非小細胞肺癌におけるエストロゲン合成・代謝酵素の発現動態とその意義

    飯田 慎也, 三木 康宏, 小野 克彦, 笹野 公伸

    日本病理学会会誌　102　(1)　421-421　2013/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  104. エストロゲン受容体陰性、プロゲステロン受容体陰性、HER2陰性の三重陰性乳がん(TNBC)患者のアンドロゲン代謝 多変量比較解析(Androgen metabolism in triple negative breast cancer (TNBC) patients-multivariate comparison of the findings)

    マクナマラ・キーリー, 依田 智美, 三木 康宏, チャンプラコン・ニラモン, ヲングァイサヤワン・サンサネエ, インチロエン・ピンピン, コンダン・ヨウワヌシュ, 王 リン, 高木 清司, 高木 まゆ, 中村 保宏, 鈴木 貴, 宮下 穣, 根本 紀子, 玉城 研太朗, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本内分泌学会雑誌　89　(1)　254-254　2013/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  105. 乳癌の転移リンパ節におけるアンドロゲン発現解析

    柴原 裕紀子, 櫻田 千佳子, 三木 康宏, 端 秀子, 中村 保宏, 笹野 公伸

    日本病理学会会誌　102　(1)　293-293　2013/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  106. PS-134-2 非浸潤性乳管癌組織におけるエストロゲン誘導遺伝子の発現(PS ポスターセッション,第113回日本外科学会定期学術集会)

    江幡 明子, 鈴木 貴, 高木 清司, 三木 康宏, 小野寺 好明, 中村 保宏, 藤島 史喜, 石田 和之, 渡辺 みか, 玉城 研太朗, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本外科学会雑誌　114　(2)　704-704　2013/03/05

    Publisher: 一般社団法人日本外科学会

    ISSN： 1880-1129

  107. 肺癌および乳癌における腫瘍マーカーとしてのp62/SQSTM1の意義

    仁平 開人, 三木 康宏, 鈴木 貴, 笹野 公伸

    日本分子腫瘍マーカー研究会誌　28　43-44　2013/03

    Publisher: 日本分子腫瘍マーカー研究会

    DOI： 10.11241/jsmtmr.28.43 　

  108. 非浸潤性乳管癌組織におけるエストロゲン誘導遺伝子の発現

    江幡 明子, 鈴木 貴, 高木 清司, 三木 康宏, 小野寺 好明, 中村 保宏, 藤島 史喜, 石田 和之, 渡辺 みか, 玉城 研太朗, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本外科学会雑誌　114　(臨増2)　704-704　2013/03

    Publisher: (一社)日本外科学会

    ISSN： 0301-4894

  109. 乳がんと性ステロイド合成阻害剤：現状と将来

    三木康宏, 笹野公伸, 伊藤 潔

    産婦人科の実際　62　(09)　1247-1253　2013

    Publisher: 金原出版株式会社

  110. Estrogen-induced genes in ductal carcinoma in situ(DCIS): their comparison with invasive ductal carcinoma.

    A. Ebata, T. Suzuki, K. Takagi, Y. Miki, Y. Onodera, Y. Nakamura, F. Fujishima, K. Ishida, M. Watanabe, K. Tamaki, T. Ishida, N. Ohuchi, H. Sasano

    CANCER RESEARCH　72　2012/12

    DOI： 10.1158/0008-5472.SABCS12-P8-05-14 　

    ISSN： 0008-5472

    eISSN： 1538-7445

  111. 乳癌におけるアロマターゼ阻害剤の投与に伴うmicroRNAの変動およびlet-7fによるCYP19A1遺伝子の制御について(Aromatase inhibitor treatment of breast cancer cells increases the expression of let-7f, a microRNA targeting CYP19A1)

    柴原 裕紀子, 三木 康宏, 端 秀子, 小野寺 好明, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　71回　231-231　2012/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  112. EGFRチロシンキナーゼ阻害剤耐性を獲得した肺癌細胞におけるオートファジーの役割(A Role for Macroautophagy in the Development of Lung Cancer Resistance to EGFR Tyrosine Kinase Inhibitor)

    仁平 開人, 三木 康宏, 飯田 慎也, 小野 克彦, 笹野 公伸

    日本癌学会総会記事　71回　294-294　2012/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  113. ER陽性乳癌におけるAhRを介したAromatase発現誘導(Induction of aromatase through Aryl hydrocarbon receptor in ER-positive breast cancer)

    齊藤 涼子, 三木 康宏, 飯田 慎也, 端 秀子, 高木 清司, 小野 克彦, 大内 憲明, 鈴木 貴, 笹野 公伸

    日本癌学会総会記事　71回　365-365　2012/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  114. ヒト結腸癌におけるRUNX2の発現意義 ERβを介した予後規定因子(RUNX2 in human colon earcinoma: a potent prognostic factor associated with estrogen receptor)

    佐瀬 友彦, 鈴木 貴, 三浦 康, 椎葉 健一, 佐藤 郁郎, 高木 清司, 小野寺 好明, 三木 康宏, 大沼 忍, 佐々木 宏之, 柴田 近, 海野 倫明, 笹野 公伸

    日本癌学会総会記事　71回　491-491　2012/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  115. トリプルネガティブ乳癌におけるアンドロゲン生成経路の活性化は腫瘍増殖能の低下に関与する(Activation of androgenic pathways results in lower tumor cell proliferation in triple negative breast cancer)

    マクナマラ・キーリー, 三木 康宏, チャンプラコン・ニラモン, ウォンワイサヤワン・サンサニー, インチャロン・ピンピン, 中村 保宏, 鈴木 貴, 宮下 穣, 玉城 研太朗, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　71回　363-363　2012/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  116. 乳癌におけるpromyelocytic zinc finger protein(PLZF)の発現(Expression of promyelocytic zinc finger protein (PLZF) in human breast carcinoma)

    高木 清司, 三木 康宏, 小野寺 好明, 中村 保宏, 石田 孝宣, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　71回　364-364　2012/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  117. 非小細胞肺癌におけるAxlのリガンド依存的および非依存的活性化(Ligand-dependent and independent activation of Axl in non-small cell lung carcinoma)

    飯田 慎也, 三木 康宏, 中村 保宏, 鈴木 貴, 岡部 尚文, 笹野 公伸

    日本癌学会総会記事　71回　464-464　2012/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  118. 膵神経内分泌腫瘍における腫瘍増殖に関与する諸因子の免疫組織学的検討

    笠島 敦子, 中村 保宏, 三木 康宏, 鈴木 貴, 笹野 公伸

    日本内分泌学会雑誌　88　(1)　263-263　2012/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  119. 乳癌におけるアロマターゼ阻害剤投与によるmicroRNAの変動およびLet-7fによるCYP19A1の制御に関する検討

    柴原 裕紀子, 三木 康宏, 端 秀子, 小野寺 好明, 中村 保宏, 赤平 純一, 阿部 佳子, 笹野 公伸

    日本病理学会会誌　101　(1)　253-253　2012/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  120. 非小細胞肺癌におけるAxlおよびGas6の発現局在と意義

    飯田 慎也, 三木 康宏, 中村 保宏, 鈴木 貴, 岡部 尚文, 笹野 公伸

    日本病理学会会誌　101　(1)　386-386　2012/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  121. 乳癌組織におけるAryl hydrocarbon receptorと臨床学的事項およびaromatase発現との関わりについての検討

    齊藤 涼子, 三木 康宏, 大庭 悠貴, 端 秀子, 中村 保宏, 鈴木 貴, 笹野 公伸

    日本病理学会会誌　101　(1)　408-408　2012/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  122. 災害産婦人科学とは何か？

    伊藤 潔, 三木康宏

    医学のあゆみ　242　(12)　949-950　2012

    Publisher: 医歯薬出版株式会社

  123. 植物性エストロゲン

    三木康宏

    CANCER BOARD 乳癌　5　(2)　39-44　2012

    Publisher: 株式会社メディカルレビュー社

  124. 乳癌におけるaryl hydrocarbon receptorと腫瘍内Estrogen産生との関係

    齊藤 涼子, 三木 康宏, 端 秀子, 飯田 慎也, 小野 克彦, 石田 孝宣, 鈴木 貴, 大内 憲明, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　19回　225-225　2011/09

    Publisher: (一社)日本乳癌学会

  125. 男子乳癌におけるエストロゲン作用

    高木 清司, 森谷 卓也, 黒住 昌史, 岡 きま子, 豊島 隆, 武井 寛幸, 平川 久, 石田 孝宣, 紅林 淳一, 林 慎一, 三木 康宏, 笹野 公伸, 鈴木 貴

    日本乳癌学会総会プログラム抄録集　19回　386-386　2011/09

    Publisher: (一社)日本乳癌学会

  126. 乳癌原発巣および転移リンパ節でのアロマターゼ免疫染色の比較検討

    柴原 裕紀子, 三木 康宏, 工藤 陽花子, 小野 克彦, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　19回　408-408　2011/09

    Publisher: (一社)日本乳癌学会

  127. ヒトER陽性乳癌におけるエストロゲン誘導性microRNA-7とEGFR発現

    増田 真理子, 三木 康宏, 端 秀子, 鈴木 貴, 石田 孝宣, 平川 久, 大内 憲明, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　19回　411-411　2011/09

    Publisher: (一社)日本乳癌学会

  128. 通常の副腎皮質、副腎腺腫、副腎癌における代謝型グルタミン酸受容体3(GRM3)評価(Metabotropic Glutamate Receptor 3 (GRM3) evaluation in Normal Adrenal Cortex, Adrenal Adenomas and Carcinoma)

    フェリゾラ・サウロ, 中村 保宏, 佐藤 文俊, 小野寺 好明, 岩渕 絵里奈, 小野 克彦, 三木 康宏, 笹野 公伸

    日本内分泌学会雑誌　87　(2)　824-825　2011/09

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  129. 乳癌におけるKLF5の発現意義(KLF5 in human breast carcinoma)

    高木 清司, 三木 康宏, 小野寺 好明, 中村 保宏, 石田 孝宣, 井上 聡, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　70回　168-168　2011/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  130. 乳癌組織におけるamyloid precursor proteinの発現(Immunolocalization of amyloid precursor protein in human breast carcinoma)

    伊藤 重宏, 高木 清司, 小野寺 好明, 三木 康宏, 中村 保宏, 石田 孝宣, 井上 聡, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　70回　169-169　2011/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  131. 非浸潤性及び浸潤性乳癌組織におけるエストロゲン作用

    江幡 明子, 鈴木 貴, 高木 清司, 三木 康宏, 五十嵐 やよい, 中村 保宏, 石田 和之, 渡辺 みか, 石田 孝宣, 大内 憲明, 笹野 公伸

    日本乳癌学会総会プログラム抄録集　19回　225-225　2011/09

    Publisher: (一社)日本乳癌学会

  132. 肺癌とエストロゲン (医学のあゆみ)

    三木康宏, 笹野公伸

    医学のあゆみ　238　(11)　1080-1081　2011/09

  133. 乳癌研究の最前線Micro RNA (月刊カレントテラピー )

    笹野公伸, 柴原裕紀子, 三木康宏

    月刊カレントテラピー　29　(5)　73-78　2011/05

  134. 骨芽細胞におけるaryl hydrocarbon receptorのエストロゲン合成への関与

    三木 康宏, 端 秀子, 小野 克彦, 鈴木 貴, 熊本 裕行, 笹野 公伸

    Clinical Calcium　21　(5)　755-756　2011/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  135. 神経内分泌腫瘍組織におけるinsulin-like growth factor-1 receptor(IGF-1R)の活性化と細胞内シグナル伝達系との関連性

    飯田 慎也, 三木 康宏, 小野 克彦, 中村 保宏, 鈴木 貴, 笹野 公伸

    日本内分泌学会雑誌　87　(1)　327-327　2011/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  136. 基礎からみた乳腺疾患 miRNAと乳癌について (CANCER BOARD 乳癌)

    柴原裕紀子, 増田真理子, 三木康宏, 笹野公伸

    CANCER BOARD 乳癌　4　(1)　39-44　2011/04

  137. 口腔扁平上皮癌における多剤耐性因子の発現 (日本病理学会会誌)

    三木康宏, 及川麻理子, 小野克彦, 清水良央, 笹野公伸, 熊本裕行

    日本病理学会会誌　100　(1)　466-466　2011/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  138. 乳癌における原発巣および転移リンパ節のアロマターゼ免疫染色の比較検討 (日本病理学会会誌)

    柴原裕紀子, 三木康宏, 阿部佳子, 笹野公伸

    日本病理学会会誌　100　(1)　399-399　2011/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  139. 間質性肺炎におけるエストロゲン受容体とアロマターゼの発現

    谷内 真司, 鈴木 貴, 三木 康宏, 阿部 佳子, 藤島 史喜, 中村 保宏, 佐藤 聡子, 笠島 敦子, 石田 和之, 渡辺 みか, 笹野 公伸

    日本病理学会会誌　100　(1)　335-335　2011/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  140. 非小細胞肺癌組織におけるAxlの活性化と発現意義

    飯田 慎也, 三木 康宏, 阿部 佳子, 小野 克彦, 中村 保宏, 鈴木 貴, 岡部 尚文, 笹野 公伸

    日本病理学会会誌　100　(1)　457-457　2011/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  141. Insulin-Like Growth Factor-1 Receptor (IGF-1R) and Its Inhibition in Non-Functioning Neuroendocrine Tumors

    H. Sasano, S. Iida, K. Ono, Y. Nakamura, Y. Miki

    NEUROENDOCRINOLOGY　94　10-10　2011

    ISSN： 0028-3835

  142. ステロイドホルモンとその受容体の組織化学 肺癌における性ステロイドホルモンの作用 (日本組織細胞化学会総会・学術集会講演プログラム・予稿集)

    三木康宏, 阿部佳子, 鈴木貴, 笹野公伸

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集　51回　33-33　2010/09

  143. 男子乳癌における性ホルモン受容体の発現(Sex-steroid receptors in male breast carcinoma)

    岡 きま子, 森谷 卓也, 黒住 昌史, 高木 清司, 三木 康宏, 豊島 隆, 武井 寛幸, 平川 久, 石田 孝宣, 紅林 淳一, 林 慎一, 笹野 公伸, 鈴木 貴

    日本癌学会総会記事　69回　458-458　2010/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  144. Immunolocalization of NUCB2 in human breast carcinoma

    Shiho Suzuki, Yoshiaki Onodera, Yasuhiro Miki, Jun-ichi Akahira, Hironobu Sasano, Takashi Suzuki

    ENDOCRINE JOURNAL　57　S578-S578　2010/03

    ISSN： 0918-8959

  145. Tissue concentration of sex steroid hormones and its correlations with clinicopathological parameters in human epithelial ovarian cancer

    Jun-ichi Akahira, Fumihiko Suzuki, Takashi Suzuki, Yasuhiro Miki, Shuji Nagasaki, Seijiro Honma, Kiyoshi Ito, Nobuo Yaegashi, Hironobu Sasano

    ENDOCRINE JOURNAL　57　S579-S579　2010/03

    ISSN： 0918-8959

  146. Intratumoral androgen levels in breast carcinoma with aromatase inhibitor exemestane: 17 beta-hydroxysteroid dehydrogenase type 2 as a marker for increased androgen actions

    Kiyoshi Takagi, Shuji Nagasaki, Yoshiaki Onodera, Yasuhiro Miki, Jun-ichi Akahira, Katsuhiko Ono, Hisashi Hirakawa, Hironobu Sasano, Takashi Suzuki

    ENDOCRINE JOURNAL　57　S578-S579　2010/03

    ISSN： 0918-8959

  147. Identification of estradiol-estrogen receptor-regulated miRNAs in breast cancer cells

    Mariko Masuda, Shuko Hata, Yasuhiro Miki, Hironobu Sasano

    ENDOCRINE JOURNAL　57　S579-S579　2010/03

    ISSN： 0918-8959

  148. Regulation of aromatase expression by intratumoral stromal cell-derived factors in lung carcinoma cells

    Yasuhiro Miki, Takashi Suzuki, Keiko Abe, Satoshi Suzuki, Shuko Hata, Jun-ichi Akahira, Hironobu Sasano

    ENDOCRINE JOURNAL　57　S592-S593　2010/03

    ISSN： 0918-8959

  149. Expression of mTOR signaling pathway and the effects of mTOR inhibitor on cell signaling pathway in neuroendocrine tumors

    Shinya Iida, Yasuhiro Miki, Katsuhiko Ono, Hironobu Sasano

    ENDOCRINE JOURNAL　57　S521-S521　2010/03

    ISSN： 0918-8959

  150. Immunolocalization of estrogen-related enzymes in benign human breast disorders

    Yoshie Sasaki, Yoshiaki Onodera, Yasuhiro Miki, Jun-ichi Akahira, Hironobu Sasano, Takashi Suzuki

    ENDOCRINE JOURNAL　57　S539-S539　2010/03

    ISSN： 0918-8959

  151. Expression of steroid sulfatase in human non-small cell lung carcinoma

    Hideaki Kakinuma, Yasuhiro Miki, Keiko Abe, Satoshi Suzuki, Jun-ichi Akahira, Takashi Suzuki, Hironobu Sasano

    ENDOCRINE JOURNAL　57　S593-S593　2010/03

    ISSN： 0918-8959

  152. Estrogen receptors in human skin-its correlation with age and gender

    Takayoshi Inoue, Yasuhiro Miki, Keiko Abe, Masahito Hatori, Masami Hosaka, Katsuhiko Ono, Miki Mori, Yoshiyuki Kariya, Setsuya Aiba, Hironobu Sasano

    ENDOCRINE JOURNAL　57　S541-S541　2010/03

    ISSN： 0918-8959

  153. 間質性肺炎におけるエストロゲン受容体とアロマターゼの発現

    谷内 真司, 鈴木 貴, 三木 康宏, 阿部 佳子, 鈴木 瑛介, 中村 保宏, 藤島 史喜, 伊藤 しげみ, 石田 和之, 渡辺 みか, 笹野 公伸

    日本病理学会会誌　99　(1)　291-291　2010/03

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  154. Estrogen Receptor and Aromatase in Squamous Cell Carcinoma of the Skin

    K. Abe, M. Mori, Y. Miki, T. Inotte, K. Ono, Y. Shibahara, J. Akahira, H. Sasano

    LABORATORY INVESTIGATION　90　110A-110A　2010/02

    ISSN： 0023-6837

  155. Estrogen Receptor and Aromatase in Squamous Cell Carcinoma of the Skin

    K. Abe, M. Mori, Y. Miki, T. Inoue, K. Ono, Y. Shibahara, J. Akahira, H. Sasano

    MODERN PATHOLOGY　23　110A-110A　2010/02

    ISSN： 0893-3952

  156. [Bone and Men's Health. Effects of aromatase inhibitors on human osteoblasts.] (Clin Calcium)

    Sasano Hironobu, Miki Yasuhiro

    Clin Calcium　20　(2)　189-197　2010/02

  157. ヒト骨芽細胞へのアロマターゼ阻害剤の影響

    笹野公伸, 三木康宏

    CLINICAL CALCIUM　20　35-43　2010

  158. Expression of mTOR Signaling Pathway and Effects of mTOR Inhibitors on Its Pathway in Human Neuroendocrine Tumors

    S. Iida, Y. Miki, K. Ono, H. Sasano

    NEUROENDOCRINOLOGY　92　(1)　8-8　2010

    ISSN： 0028-3835

  159. 【テストステロンと骨】 ヒト骨芽細胞へのアロマターゼ阻害剤の影響 (Clinical Calcium)

    笹野公伸, 三木康宏

    Clinical Calcium　20　(2)　189-197　2010/01

  160. Estrogen receptors and aromatase in Japanese female patients with pulmonary adenocarcinoma: a comparison between BAC and non-BAC

    K. Abe, Y. Miki, K. Ono, N. Kikuchi, S. Suzuki, T. Takahashi, H. Sasano

    VIRCHOWS ARCHIV　455　345-346　2009/08

    ISSN： 0945-6317

  161. ヒト骨組織におけるaryl hydrocarbon receptorの発現

    三木 康宏, 端 秀子, 長崎 修治, 鈴木 貴, 笹野 公伸

    The Journal of Toxicological Sciences　34　(Suppl.)　S103-S103　2009/06

    Publisher: (一社)日本毒性学会

    ISSN： 0388-1350

  162. 10%ホルマリン固定パラフィン包理標本から抽出したTotalRNAおよびmicroRNAの質的検討 (日本病理学会会誌)

    三浦伊久美, 三木康宏, 赤平純一, 鈴木貴, 笹野公伸

    日本病理学会会誌　98　(1)　345-345　2009/03

  163. 皮膚扁平上皮癌におけるホルモン受容体とアロマターゼの発現の検討 (日本病理学会会誌)

    森美紀, 阿部桂子, 小野克彦, 三木康宏, 赤平純一, 笹野公伸

    日本病理学会会誌　98　(1)　340-340　2009/03

  164. 臨床的にDiGeorge症候群が疑われたEBV陽性T細胞性悪性リンパ腫の1剖検例 (日本病理学会会誌)

    伊藤しげみ, 三木康宏, 笹野公伸, 一迫玲

    日本病理学会会誌　98　(1)　273-273　2009/03

  165. Estrogen receptor beta in non-small cell lung cancer a significant correlation with intratumoral aromatase

    K. Abe, Y. Miki, M. Mori, Y. Kou, N. Kudo, M. Koguchi, H. Sasano

    HISTOPATHOLOGY　53　336-337　2008/10

    ISSN： 0309-0167

  166. 上皮性卵巣癌における組織中性ステロイドホルモン濃度と臨床病理学的因子の関連について(Tissue concentration of sex steroid hormones and its clinicopathological significance in epithelial ovarian cancer)

    赤平 純一, 鈴木 史彦, 鈴木 貴, 伊藤 潔, 三木 康宏, 長崎 修治, 八重樫 伸生, 笹野 公伸

    日本癌学会総会記事　67回　197-197　2008/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  167. 乳癌組織におけるアロマターゼとその発現調節機構に関する研究

    三木 康宏

    東北医学雑誌　120　(1)　54-57　2008/06/25

    ISSN： 0040-8700

  168. ヒト骨組織におけるSteroid and Xenobiotic Receptorの発現とその作用

    三木 康宏, 長崎 修治, 端 秀子, 赤平 純一, 鈴木 貴, 笹野 公伸

    The Journal of Toxicological Sciences　33　(Suppl.)　S88-S88　2008/06

    Publisher: (一社)日本毒性学会

    ISSN： 0388-1350

  169. 内分泌攪乱物質と骨 (医学のあゆみ)

    三木康宏, 笹野公伸

    医学のあゆみ　224　(11)　873-874　2008/03/15

  170. アロマターゼの基礎と臨床 (HORMONE FRONTIER IN GYNECOLOGY )

    笹野公伸, 三木康宏

    HORMONE FRONTIER IN GYNECOLOGY　15　(1)　11-14　2008/03

  171. 乳癌におけるNUDT2の発現に関する免疫組織化学的検討

    岡きま子, 鈴木貴, 赤平純一, 三木康宏, 小野寺好明, 小野克彦, 長崎修治, 森美紀, 石田孝宣, 大内憲明, 笹野公伸

    日本癌学会総会記事　67回　223-223　2008

  172. New development in intracrinology of breast carcinoma: Therapeutic horizons after aromatase inhibitors

    Hironobu Sasano, Yasuhiro Miki, Tsuyoshi Fukuda, Shuji Nagasaki, Taisuke Nakata, Takashi Suzuki

    Expert Review of Endocrinology and Metabolism　2　(3)　367-374　2007/06

    DOI： 10.1586/17446651.2.3.367 　

    ISSN： 1744-6651

  173. 【乳癌 基礎・臨床研究のアップデート】 基礎研究 病理・分子生物学 乳癌における局所ホルモン環境とアロマターゼ (日本臨床)

    笹野公伸, 三木康宏

    日本臨床　65　(増刊6 乳癌)　81-86　2007/06

    Publisher: 日本臨床社

    ISSN： 0047-1852

  174. ヒト骨芽細胞における内分泌攪乱物質の影響

    三木 康宏, 長崎 修治, 赤平 純一, 五十嵐 勝秀, 菅野 純, 鈴木 貴, 笹野 公伸

    The Journal of Toxicological Sciences　32　(Suppl.)　S196-S196　2007/05

    Publisher: (一社)日本毒性学会

    ISSN： 0388-1350

  175. 前立腺癌におけるEstrogen Receptor β及びβcxの機能解析

    長崎 修治, 中村 保宏, 三木 康宏, 赤平 純一, 鈴木 貴, 林 慎一, 笹野 公伸

    日本内分泌学会雑誌　83　(1)　148-148　2007/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  176. ヒト上皮性卵巣癌におけるEstrogen related receptor(ERR)αの発現と臨床病理学的因子との関連について

    赤平 純一, 鈴木 貴, 三木 康宏, 長崎 修治, 伊藤 潔, 森谷 卓也, 林 慎一, 八重樫 伸生, 笹野 公伸

    日本内分泌学会雑誌　82　(2)　425-425　2006/09

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  177. 卵巣癌におけるRIZ1の発現と遺伝子プロモーター領域のDNAメチル化について

    赤平 純一, 鈴木 貴, 伊藤 潔, 森谷 卓也, 八重樫 伸生, 三木 康宏, 長崎 修治, 笹野 公伸

    日本癌学会総会記事　65回　198-198　2006/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  178. 乳癌組織におけるアンドロゲン濃度

    鈴木 貴, 三木 康宏, 森谷 卓也, 赤平 純一, 石田 孝宣, 平川 久, 山口 ゆり, 林 慎一, 笹野 公伸

    日本内分泌学会雑誌　82　(2)　417-417　2006/09

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  179. 前立腺癌細胞におけるEstrogen Receptor beta及びbeta cxの機能解析

    長崎 修治, 中村 保宏, 三木 康宏, 赤平 純一, 鈴木 貴, 林 慎一, 笹野 公伸

    日本内分泌学会雑誌　82　(2)　423-423　2006/09

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  180. 乳癌組織における核内受容体LRH-1の発現意義

    鈴木 貴, 三木 康宏, 渋谷 里絵, 中村 保宏, 森谷 卓也, 笹野 公伸

    日本病理学会会誌　95　(1)　235-235　2006/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  181. 乳癌組織における核内受容体peroxisome proliferator-activated receptor(PPAR)γの発現意義

    鈴木貴, 三木康宏, 森谷卓也, 石田孝宣, 菅原明, 林慎一, 大内憲明, 笹野公伸

    日本内分泌学会雑誌　82　(1)　108-108　2006

  182. ヒト乳癌アロマターゼの腫瘍内局在と発現機序の検討

    三木康宏, 鈴木貴, 山口ゆり, 森谷卓也, 林慎一, 大内憲明, 笹野公伸

    日本癌学会総会記事　65回　340-340　2006

    Publisher: 日本癌学会

    ISSN： 0546-0476

  183. カルチノイド腫瘍におけるソマトスタチン受容体(SSTR)の発現

    小野 克彦, 鈴木 貴, 三木 康宏, 谷山 裕亮, 中村 保宏, 笹野 公伸

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集　46回　(46)　64-64　2005/10

    Publisher: 日本組織細胞化学会

  184. カルチノイド細胞におけるソマトスタチンの作用

    小野 克彦, 鈴木 貴, 三木 康宏, 谷山 裕亮, 中村 保宏, 笹野 公伸

    日本内分泌学会雑誌　81　(1)　168-168　2005/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  185. 内分泌攪乱物質のヒト骨芽細胞に対する影響 マイクロアレイによる遺伝子発現解析

    三木 康宏, 鈴木 貴, 五十嵐 勝秀, 相崎 健一, 菅野 純, 中村 保宏, 井上 達, 笹野 公伸

    日本内分泌学会雑誌　81　(1)　129-129　2005/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  186. IA-29 Expression of somatostatin receptor (SSTR) in human carcinoid tumor(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY) :

    Ono Katsuhiko, Suzuki Takashi, Miki Yasuhiro, Taniyama Yuusuke, Nakamura Yasuhiro, Sasano Hironobu

    Acta histochemica et cytochemica　38　(6)　S18　2005

    Publisher: Japan Society of Histochemistry and Cytochemistry

  187. Effects of aromatase inhibitors on human osteoblast cells: a possible androgenic bone protective effects induced by exemestane

    Y Miki, T Suzuki, K Igarashi, J Kanno, H Sasano

    BREAST CANCER RESEARCH AND TREATMENT　94　S204-S204　2005

    ISSN： 0167-6806

  188. 乳癌組織におけるestrogen-related receptor α(ERRα)の発現

    鈴木貴, 三木康宏, 森谷卓也, 石田孝宣, 井上聡, 大内憲明, 笹野公伸

    日本内分泌学会雑誌　81　(1)　168-168　2005

  189. Steroid and Xenobiotic Receptor(SXR),CYP3A4,MDR1の乳癌組織における発現

    三木 康宏, 鈴木 貴, 田澤 智香, 松井 恵, 石田 孝宣, 平川 久, Blumberg Bruce, 笹野 公伸

    日本内分泌学会雑誌　80　(2)　409-409　2004/09

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  190. ヒト乳癌組織におけるperoxisome proliferator-activated receptor(PPAR)γの発現

    鈴木貴, 中林正雄, 三木康宏, 森谷卓也, 石田孝宣, 大内憲明, 笹野公伸

    日本乳癌学会総会プログラム抄録集　12回　175-175　2004

  191. ヒト乳癌組織におけるliver receptor homologue-1(LRH-1)の発現

    斉藤 涼子, 鈴木 貴, 三木 康宏, 石田 孝宣, 森谷 卓也, 大内 憲明, 笹野 公伸

    日本癌学会総会記事　62回　443-443　2003/08

    Publisher: 日本癌学会

    ISSN： 0546-0476

  192. ヒト乳癌におけるsteroid sulfatase(STS)及びestrogen sulfotransferase(EST)の発現意義

    鈴木 貴, 三木 康宏, 田澤 智香, 中田 泰介, 塩津 行正, 秋永 士朗, 石田 孝宣, 森谷 卓也, 笹野 公伸

    日本病理学会会誌　92　(1)　221-221　2003/04

    Publisher: (一社)日本病理学会

    ISSN： 0300-9181

  193. ヒト大動脈におけるPRの発現及びプロゲステロンの局所産生

    中村 保宏, 鈴木 貴, 井上 宰, 三木 康宏, 田澤 智香, 小野 克彦, 松井 恵, 森谷 卓也, 笹野 公伸

    日本内分泌学会雑誌　79　(1)　134-134　2003/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

  194. 動脈硬化病変でヒト大動脈に発現するエストロゲンレセプターのサブタイプの検討

    中村保宏, 鈴木貴, 三木康宏, 金子智香, 笹野公伸

    日本内分泌学会雑誌　78　(1)　106　2002/04/20

    ISSN： 0029-0661

  195. ヒト乳癌組織におけるsteroid sulfatase及びestrone sulfotransferaseの発現

    鈴木 貴, 三木 康宏, 森谷 卓也, 石田 孝宣, 中田 泰介, 塩津 行正, 秋永 士朗, 井上 謙吾, 木村 道夫, 笹野 公伸

    日本内分泌学会雑誌　78　(1)　138-138　2002/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

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Books and Other Publications 17

  1. 生体統御システムと内分泌攪乱 (SPRINGER REVIEWS)

    三木康宏, 鈴木 貴, 笹野公伸

    シュプリンガーフェアラーク東京　2023/05/25

    ISBN: 4431711120

  2. 地球防災ラボ : 実験でしくみを知って、命を守る

    東北大学災害科学国際研究所

    岩崎書店　2022/09/30

    ISBN: 9784265059775

  3. 苦悩する女性研究者

    三木康宏

    日本経済新聞　2021/04/26

  4. 東日本大震災からのスタート: 災害を考える51のアプローチ

    東北大学災害科学国際研究所

    東北大学出版会　2021/03/18

    ISBN: 4861633575

  5. 研修ノート 災害時における周産期医療

    公益財団法人日本産婦人科医会

    2021

  6. コロナ禍の行動規制が研究者の不安を煽る

    三木康宏

    Medical Tribune　2020/11/27

  7. 新型コロナ対策の行動規制, 研究者にも大きな影響 東北大学が調査

    三木康宏

    大学ジャーナル　2020/11/24

  8. 東北大, 新型コロナウイルス感染症対策としての行動規制（COVID-19規制）が研究者に及ぼした影響の実態調査結果を発表

    日本経済新聞　2020/11/19

  9. DISASTERS LEAD TO REDUCTIONS IN CANCER SCREENING

    Miki Yasuhiro

    ScienceDaily　2020/05/07

  10. Gynecologic Cancer Screening After a Natural Disaster.

    Miki Yasuhiro

    The ASCO post　2020/04/01

  11. Disasters Can Affect Cervical Cancer Screening for Years

    Miki Yasuhiro

    2020/03/27

  12. Disasters can affect cervical cancer screening for years.

    Miki Yasuhiro

    2020/03/27

  13. 東北大, 東日本大震災後の子宮頸がん検診の受診率回復に地域格差があることを解明

    三木康宏

    日本経済新聞　2020/03/13

  14. 災害起きたら ペットもいっしょに避難

    三木康宏

    朝日小学生新聞　2020/02/12

  15. Interface Oral Health Science 2011

    Yasuhiro MIKI, Shuko HATA, Ryoko SAITO, KatsuhikoONO, Hironobu SASANO, Hiroyuki KUMAMOTO

    Springer Japan KK.　2012/03/27

  16. 生体統御システムと内分泌撹乱, Springer Reviews

    井上 達, 井口 泰泉, 三木康宏, 鈴木 貴, 笹野公伸

    シュプリンガー・フェアラーク東京株式会社　2005/05

  17. Advances in Endocrine Therapy of Breast Cancer

    Sasano H, Edwards DP, Anderson TJ, Silverberg SG, Evans DB, Santen RJ, Ramage P, Miki Y, Suzuki T, Simpson ER, Bhatnagar AS, Miller WR

    Marcel Dekker Inc.　2004/05

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Presentations 420

  1. The roles of asporin in cancer-associated fibroblasts of lung adenocarcinoma

    The 114th Annual Meeting of the Japanese Society of Pathology　2025/04/19

  2. Significance of Cadherin 6 expression in endometrial carcinoma

    The 114th Annual Meeting of the Japanese Society of Pathology　2025/04/19

  3. Clinicopathological significance of SPHK1 and TYMP expressions in pulmonary lymphangioleiomyomatosis

    The 114th Annual Meeting of the Japanese Society of Pathology　2025/04/19

  4. Lower transglutaminase 2 expression in fibroblastic foci of usual interstitial pneumonia pattern

    The 114th Annual Meeting of the Japanese Society of Pathology　2025/04/19

  5. TROP2 expression in breast cancer subtypes

    The 114th Annual Meeting of the japanese Society of Pathology　2025/04/19

  6. トリプルネガティブ乳癌におけるホルモン濃度とTリンパ球サブセットの関係

    The 114th Annual Meeting of the japanese Society of Pathology　2025/04/19

  7. Development of analytical method of FOXL2 mutation detection in adult granulosa cell tumor

    The 114th Annual Meeting of the Japanese Society of Pathology　2025/04/18

  8. Spatial transcriptomics revealed low ITGA11 in the stroma of lung cancer with interstitial pneumonia

    The 114th Annual Meeting of the Japanese Society of Pathology　2025/04/18

  9. Significance of ferroportin expression in ovarain cancer

    The 114th Annual Meeting of the Japanese Society of Pathology　2025/04/19

  10. 加齢および高脂肪食負荷が骨髄脂肪細胞の特性に与える影響

    海野 雄加, 村上 昌平, 三木 康宏, 山口 壹範, 谷 春菜

    日本薬学会第145年会　2025

  11. トリプルネガティブ乳癌における腫瘍内ホルモン濃度とTリンパ球サブセットの関係

    山崎 あすみ, 三木 康宏, 岩渕 英里奈, 西脇 良紀, 井上 千裕, 江幡 明子, 石田 孝宣, 鈴木 貴

    第32回ステロイドホルモン学会学術集会　2025/02/22

  12. 乳癌におけるステロイドホルモンシグナルの可視化

    三木 康宏, 岩渕 英里奈, 井上 千裕, 山﨑 有人, 鈴木 貴

    第99回日本病理学会東北支部学術集会　2024/12/07

  13. 間質性肺炎合併肺腺癌における癌関連線維芽細胞と細胞外基質の特徴

    井上千裕, 三木康宏, 青山弥生, 柴田沙織, 野津田 泰嗣, 岡田克典, 鈴木貴

    第99回日本病理学会東北支部学術集会　2024/12/07

  14. 乳癌の診断、治療と研究における組織細胞化学

    三木康宏, 岩渕英里奈, 山崎あすみ, 井上千裕, 鈴木貴

    第65回日本組織細胞化学会総会・学術集会　2024/10/27

  15. HER2結合タンパクによるHER2低発現乳癌の特徴の解明

    岩渕英里奈, 三木康宏, 石井博子, 山崎あすみ, 石田孝宣, 鈴木貴

    第65回日本組織細胞化学会総会・学術集会　2024/10/26

  16. Lipid metabolism in endometrial cancer: significance of carnitine palmitoyltransferase 2 in cancer malignant behavior

    Yasuhiro Miki, Erina Iwabuchi, Hiroko Ishii, Kiyohsi Takagi, Muneaki Shimada, Kiyoshi Ito, Takashi Suzuki

    THE 83RD ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2024/10/21

  17. The clinicopathological significance of zinc transporters in breast cancer

    Erina Iwabuchi, Yasuhiro Miki, Takanori Ishida, Takashi Suzuki

    THE 83RD ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2024/10/19

  18. The pro-tumorigenic role of Tim1 in human breast cancer

    Mai Sawafuji, Mio Tanaka, Kiyoshi Takagi, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Takashi Suziki

    THE 83RD ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2024/10/19

  19. Infiltration of TIM4 expressing antigen-presenting cells is associated with worse prognosis in breast cancer

    Mio Tanaka, Kiyoshi Takagi, Miyu Takahashi, Ai Sato, YasuhiroMiki, Minoru Miyashita, Takashi Suzuki

    THE 83RD ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2024/10/19

  20. Intratumoral cortisol and 11b-hydroxysteroid dehydrogenase 1 expression in estrogen receptor-negative breast cancer

    Ryoki Nishiwaki, Yasuhiro Miki, Erina Iwabuchi, Asumi Yamazaki, Akiko Ebata, Takanori Ishida, Takashi Suzuki

    THE 83RD ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2024/10/19

  21. Significance of Hyaluronan-related Molecules Expression in Breast Cancer

    Shiori Fujisawa, Kiyoshi Takagi, Mio Tanaka, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Hiroshi Tada, Takashi Suzuki, Takanori Ishida

    THE 83RD ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2024/10/19

  22. Spatial transcriptomics revealed features of cancer-stroma interactions in lung cancer with interstitial pneumonia

    Chihiro Inoue, Yasuhiro Miki, Yayoi Aoyama, Saori Shibata, Yoshinori Okada, Takashi Suzuki

    THE 83RD ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2024/10/19

  23. 子宮内膜癌の脂質代謝におけるカルニチン・パルミトイルトランスフェラーゼ2の意義

    三木 康宏, 岩渕 英里奈, 石井 博子, 高木 清司, 島田 宗昭, 伊藤 潔, 鈴木 貴

    第83回日本癌学会学術総会　2024/09/21

  24. 乳癌における亜鉛トランスポーターの発現意義に関する検討

    岩渕 英里奈, 三木 康宏, 石田 孝宣, 鈴木 貴

    第83回日本癌学会学術総会　2024/09/19

  25. 乳癌の進展におけるTim1の役割

    澤藤 真衣, 田中(山口) 美桜, 高木 清司, 佐藤 和, 三木 康宏, 宮下 穣, 鈴木 貴

    第83回日本癌学会学術総会　2024/09/19

  26. TIM4陽性抗原提示細胞の浸潤は乳癌の予後不良に寄与する

    田中(山口) 美桜, 高木 清司, 高橋 美佑, 佐藤 和, 三木 康宏, 宮下 穣, 鈴木 貴

    第83回日本癌学会学術総会　2024/09/19

  27. 西脇 良紀、三木 康宏、岩渕 英里奈、山崎 あすみ、江幡 明子、石田 孝宣、鈴木 貴

    エストロゲン受容体陰性乳癌における腫瘍組織中のコルチゾールと, hydroxysteroid dehydrogenase, 発現

    2024/09/19

  28. 乳癌におけるヒアルロン酸関連分子の発現意義

    藤沢 詩織, 高木 清司, 田中(山口) 美桜, 佐藤 和, 三木 康宏, 宮下 穣, 多田 寛, 鈴木 貴, 石田 孝宣

    第83回日本癌学会学術総会　2024/09/19

  29. 空間トランスクリプトーム解析による間質性肺炎合併肺癌における癌とαSMA陽性間質の相互作用に関する検討

    井上 千裕, 三木 康宏, 青山 弥生, 柴田 沙織, 岡田 克典, 鈴木 貴

    第83回日本癌学会学術総会　2024/09/19

  30. タンパク質間相互作用可視化技術の実践

    岩渕英里奈, 三木康宏, 小野克彦, 鈴木貴

    第49回組織細胞化学講習会　2024/08/09

  31. 間質性肺炎合併肺腺癌における癌細胞-癌関連線維芽細胞相互作用に関する検討

    井上千裕, 三木康宏, 青山弥生, 柴田沙織, 岡田克典, 鈴木貴

    第20回日本病理学会カンファレンス　2024/07/27

  32. エストロゲン受容体（ER）陰性乳癌組織におけるホルモン濃度と細胞老化との関係

    山崎あすみ, 三木康宏, 岩渕英里奈, 江幡明子, 多田寛, 原田成美, 濱中洋平, 宮下穣, 佐藤未来, 本成登貴和, 柳垣美歌, 昆智美, 坂本有, 鈴木貴, 石田孝宣

    第32回日本乳癌学会学術総会　2024/07/11

  33. 乳癌の化学療法耐性における低分子量ヒアルロン酸とRHAMMの相互作用

    藤沢詩織, 髙木清司, 三木康宏, 宮下穣, 多田寛, 石田孝宜, 鈴木貴

    第32回日本乳癌学会学術総会　2024/07/12

  34. 災害を契機に「なぜ研究をするのか？」を考える Invited

    三木康宏

    令和6年度第1回生物・生命科学群全体会　2024/06/28

  35. 肺腺癌での癌関連線維芽細胞におけるintegrin発現と臨床病理学的因子の相関についての検討

    柴田 沙織, 井上 千裕, 三木 康宏, 野津田 泰嗣, 新井川 弘道, 岡田 克典, 鈴木 貴

    第41回日本呼吸器外科学会学術集会　2024/05/31

  36. 肺腺癌の癌関連線維芽細胞におけるインテグリンファミリー発現と生命予後との関連性

    The 113rd Annual Meeting of the japanese Society of Pathology　2024/03/24

  37. 肺腺癌での癌関連線維芽細胞におけるintegrin発現について

    The 64th Annual Meeting of the Japan Lung Cancer Society　2023/11/03

  38. 乳癌随伴マクロファージにおける膜貫通型受容体TIM4は腫瘍免疫制御を介して乳癌の進展に寄与する

    田中(山口)美桜, 髙木清司, 髙橋美佑, 佐藤和, 三木康宏, 宮下穣, 石岡千加史, 鈴木貴

    第64回日本組織細胞化学会総会・学術集会

  39. 乳癌におけるVersicanおよびADAMTS1の発現意義

    髙木清司, 田中(山口)美桜, 佐藤和, 古川和樹, 藤田愛海, 海谷沙綾香, 小野寺好明, 三木康宏, 宮下穣, 鈴木貴

    第64回 日本組織細胞化学会総会・学術集会

  40. 乳癌の病態へのタンパク質間相互作用可視化技術を用いたアプローチ

    岩渕英里奈, 三木康宏, 笹野公伸, 鈴木貴

    第64回日本組織細胞化学会総会・学術集会

  41. 腫瘍組織を用いたステロイドホルモン・シグナルの可視化

    三木康宏, 岩渕英里奈, 鈴木貴

    第64回日本組織細胞化学会総会・学術集会　2023/10/21

  42. 子宮内膜癌における脂質代謝：carnitinepalmitoyltransferase2の意義

    三木康宏, 岩渕英里奈, 石井博子, 髙木清司, 伊藤潔, 鈴木貴

    第64回 日本組織細胞化学会総会・学術集会　2023/10/20

  43. HMGB1/TLR2,4 axis is associated with therapeutic resistance of human breast cancer

    Kiyoshi Takagi, Reina Taguchi, Mio Tanaka, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Takashi Suzuki

    THE 82ND ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2023/09/23

  44. Prognostic role of chondroitin sulfate-C in breast cancer

    Yumeko Sato, Kiyoshi Takagi, Mio Tanaka, Shiori Fujisawa, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Takashi Suzuki

    THE 82ND ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2023/09/23

  45. The role of 14-3-3ξ in breast cancer chemoresistance

    Iku Takahashi, Kiyoshi Takagi, Mio Tanaka, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Takashi Suzuki

    THE 82ND ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2023/09/23

  46. Expression of SGLT1 and SGLT2 in Breast Cancer and Potential Anticancer Effects of SGLT1 Inhibitors

    Satoko Tsunokake, Erina Iwabichi, Yasuhiro Miki, Ayako Kanai, Yoshiaki Onodera, Takanori Ishida, Hironobu Sasano, Takashi Suzuki

    THE 82ND ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2023/09/23

  47. The role of RHAMM and Hyaluronan in human breast carcinoma

    Shiori Fujisawa, Kiyoshi Takagi, Mio Tanaka, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Hiroshi Tada, Takashi Suzuki, Takanori Ishida

    THE 82ND ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2023/09/23

  48. The role of the phosphatidylserine receptor TIM4 expressed on macrophages in breast cancers.

    Mio Tanaka, Kiyoshi Takagi, Miyu Takahashi, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Chikashi Ishioka, Takashi Suzuki

    THE 82ND ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2023/09/23

  49. Dipeptidyl peptidase 4-positive cancer-associated fibroblasts enhance lung adenocarcinoma growth

    Chihiro Inoue, Yasuhiro Miki, Ryoko Koyama, Yoshinori Okada, Hironobu Sasano, Takashi Suzuki

    THE 82ND ANNUAL MEETING OF JAPANESE CANCER ASSOCIATION　2023/09/22

  50. 乳癌における模型アンドゲン受容体ZIP9の発現意義

    田中(山口)美桜, 橋場克幸, 髙木清司, 佐藤和, 江幡明子, 三木康宏, 宮下穣, 鈴木貴

    第41回日本内分泌学会内分泌代謝学サマーセミナー

  51. 乳癌におけるRHAMMの発現意義

    藤沢詩織, 髙木清司, 田中(山口)美桜, 佐藤和, 三木康宏, 宮下穣, 多田寛, 石田孝宣, 鈴木貴

    第31回日本乳癌学会学術総会

  52. 肺腺癌の癌細胞と間質におけるvasohibin-2発現に関する検討

    井上千裕, 熱海南帆, 三木康宏, 小山涼子, 岡田克典, 鈴木貴

    第112回日本病理学会総会

  53. 肺腺癌間質における弾性線維、膠原線維の割合と癌関連線維芽細胞マーカーの発現に関する検討

    浅岡 祈里, 井上 千裕, 三木 康宏, 小山 涼子, 岡田 克典, 鈴木 貴

    日本病理学会会誌　2023/03

  54. Fatty acid-related factors in breast and endometrial cancers

    Miki Y, Naraoka H, Iwabuchi E

    ENDO2022 −The 104th Annual Meeting of The Endocrine Society　2022

  55. 子宮内膜癌におけるIntracrinology

    三木康宏

    仙台内分泌フォーラム 2022（シンポジウム）　2022

  56. 災害ストレスの健康への影響

    三木康宏

    第30回特定非営利法人東北内分泌研究会（教育講演）　2022

  57. 子宮内膜癌のIntracrinology：アンドロゲンとエストロゲン

    三木康宏

    日本アンドロロジー学会 第41回学術大会（教育講演）　2022

  58. The exosome marker CD63 and Caveolin-1 in breast cancer patients

    Iwabuchi E, Miki Y, Kanai A, Ishida T, Sasano H

    American Association for Cancer Research Annual Meeting 2021　2021

  59. Correlation between T cell infiltration subtypes and intratumoral hormone levels in endometrial cancer

    Yasuhiro Miki, Fuka Onuma, Kiyoshi Takagi, Erina Iwabuchi, Takashi Suzuki, Hironobu Sasano, Kiyoshi Ito

    American Association for Cancer Research Annual Meeting 2021　2021

  60. Microtubule-Associated Protein 2 as a DHEA Binding Protein in Endometrial Cancer

    Yasuhiro Miki, Erina Iwabuchi, Kiyoshi Takagi, Takashi Suzuki, Hironobu Sasano, Kiyoshi Ito

    ENDO2021 −The 103th Annual Meeting of The Endocrine Society　2021

  61. One Health：ヒトと動物の感染症

    三木康宏

    歴史が導く災害科学の新展開Ⅳ －先人の疫病文化に学ぶ－（招待講演）　2021

  62. 子宮内膜癌におけるMAP2の発現とDHEAとの関係

    三木康宏, 岩渕英里奈, 髙木清司, 鈴木 貴, 笹野公伸, 伊藤 潔

    第61回日本組織細胞化学会総会・学術集会 （WEB開催）

  63. VEGFR Family and Vasohibin in Lymphangioleiomyomatosis (LAM) Cells Associated with its Early Onset and Progression

    Inoue C, Miki Y, Saito R, Kobayashi K, Okada Y, Sasano H.

    109th Annual Meeting of the United States and Canadian Academy of Pathology (USCAP), 2020　2020

  64. 子宮内膜癌におけるKrüppel-like factor 5 (KLF5) の発現

    三木康宏, 髙木清司, 鈴木 貴, 伊藤 潔

    第24回 日本生殖内分泌学会学術集会　2020

  65. 災害を契機に ｢なぜ研究をするのか」 を考える

    三木康宏

    超異分野学会 関西フォーラム　2020

  66. ホルモン依存性癌におけるintracrineとホルモン受容体シグナルの可視化

    三木康宏, 岩渕英里奈, 鈴木 貴

    第93回 日本内分泌学会学術集会（特別講演）　2020

  67. 令和パンデミックの今, 大正パンデミックを計量書誌学的に考察する

    三木康宏, 中鉢奈津子, 川内淳史

    第25回日本生殖内分泌学会学術集会（教育講演）　2020

  68. 子宮内膜癌におけるステロイドホルモンとKrüppel-like factor 5 の発現

    三木 康宏, 吉田 伶奈, 髙木 清司, 鈴木 貴, 伊藤 潔

    第92回 日本内分泌学会学術集会　2019/05

  69. ホルモン依存性癌におけるホルモンシグナルの可視化

    三木康宏, 岩渕英里奈, 鈴木 貴

    第92回 日本内分泌学会学術集会（特別講演）　2019

  70. Protein-protein interaction検出技術によるステロイドホルモン・シグナルの可視化

    第 27 回日本ステロイドホルモン学会学術集会（招待講演）　2019

  71. 子宮内膜癌におけるDehydroepiandrosteroneの組織中濃度とその意義

    三木 康宏, 吉田 伶奈, 高木 清司, 鈴木 貴, 伊藤 潔

    第23回 日本生殖内分泌学会学術集会　2018/12

  72. 子宮内膜癌組織におけるホルモン濃度とKrüppel-like factor 5の発現

    三木 康宏, 吉田 伶奈, 髙木 清司, 鈴木 貴, 伊藤 潔

    第19回ホルモンと癌研究会（ワークショップ）　2018

  73. Intratumoral Level of Dehydroepiandrosterone and Its Role in Endometrial Cancer Cell Proliferation

    Yoshida R, Miki Y (Presentation), Fue M, Takahashi S, Takagi K, Suzuki T, Ito K.

    ENDO2018－The 100th Annual Meeting of The Endocrine Society－　2018

  74. Intratumoral Steroid Hormone and Krüppel-Like Factor 5 Expression in Endometrial Cancer

    Miki Y, Fue M, Yoshida R, Shimizu M, Takagi K, Suzuki T, Ito K.

    ENDO2018－The 100th Annual Meeting of The Endocrine Society－　2018

  75. Annexin A1 Induced By Intratumoral Cortisol in Endometrial Cancer Microenvironment

    Miki Y, Fue M, Takagi K, Suzuki T, Ito K

    ENDO2017－The 99th Annual Meeting of The Endocrine Society　2017

  76. The 17β-Hydroxysteroid Dehydrogenase Type 2 Expression Induced by the Androgen Signal in Endometrial Cancer

    Hashimoto C, Miki Y, Tanaka S, Takagi K, Fue M, Doe Z, Li B, Suzuki T, Ito K, Yaegashi N.

    64th Annual Meeting of the Society for Reproductive Investigation　2017

  77. 婦人科悪性腫瘍に合併する悪性腫瘍：日本病理学会剖検輯報データベースを用いた解析

    三木康宏, 菅原由美, 柴原裕紀子, 辻 一郎, 笹野公伸, 伊藤 潔

    第26回日本婦人科がん検診学会総会・学術講演会　2017

  78. Effect of Intratumoral Cortisol on Aromatase Expression in Cancer Stromal Cells of Endometrial Carcinoma

    Yasuhiro Miki, Misaki Fue, Kiyoshi Takagi, Takashi Suzuki, Hironobu Sasano, Kiyoshi Ito

    ENDO2016－The 98th Annual Meeting of The Endocrine Society－　2016/04/01

  79. 子宮内膜癌におけるコルチゾールの意義

    三木康宏, 笛 未崎, 髙木清司, 橋本千明, 鈴木 貴, 伊藤 潔

    第20回特定非営利活動法人東北内分泌研究会総会（第32 回日本内分泌学会東北地方会）　2016

  80. 子宮内膜癌組織におけるコルチゾールによる aromatase の誘導

    三木康宏, 笛未崎, 髙木清司, 鈴木貴, 笹野公伸, 伊藤潔

    第17回ホルモンと癌研究会（ワークショップ）　2016/06/24

  81. 培養細胞をみる ー免疫細胞化学から電子顕微鏡までー

    三木康宏, 岩渕英里奈, 笹野公伸

    第41回日本組織細胞化学会主催 組織細胞化学講習会（特別講演）　2016

  82. Cortisol and S100P in Human Endometrial Carcinoma

    Miki Y, Fue M, Hasegawa M, Takagi K, Suzuki T, Sasano H, Ito K

    ENDO2015－The 97th Annual Meeting of The Endocrine Society－　2015

  83. Involvement of the stress hormone, cortisol, in the progression of endometrial cancer

    Yasuhiro MIKI, Misaki Fue, Kiyoshi TAKAGI, Takashi SUZUKI, Hironobu SASANO, Kiyoshi ITO

    2015

  84. 子宮内膜癌組織におけるコルチゾール濃度とエストロゲン合成との関係

    三木康宏, 笛 未崎, 髙木清司, 鈴木 貴, 伊藤 潔

    第19回特定非営利活動法人東北内分泌研究会総会（第31 回日本内分泌学会東北地方会）　2015

  85. 喫煙によるエストロゲン環境の撹乱に関する研究

    公益財団法人ひと・健康・未来研究財団 第12回助成研究発表会　2014/12/13

  86. Identification of Psoriasin (S100A7) as a key factor during interaction of cancer and adipocytes in breast tumor microenvironment. International-presentation

    Minako SAKURAI, Yasuhiro MIKI, Kiyoshi TAKAGI, Takashi SUZUKI, Hironobu SASANO

    NIH-Japan-JSPS Symposium　2014/10/23

  87. エストロゲンレセプターダイマーの発現局在の可視化とパターン解析

    岩渕英里奈, 三木康宏, 小野寺好明, 小野克彦, 鈴木 貴, 笹野公伸

    第55回日本組織細胞化学会総会・学術集会　2014/09/27

  88. S100A7 in breast carcinoma invasion into adipose tissue

    Minako SAKURAI, Yasuhiro MIKI, Takashi SUZUKI, Hironobu SASANO

    第73回日本癌学会学術総会　2014/09/25

  89. Detection of estrogen-induced dimerization patterns of estrogen receptor in breast carcinoma cells

    Erina IWABUCHI, Yasuhiro MIKI, Yoshiaki ONODERA, Katsuhiko ONO, Hironobu SASANO

    第73回日本癌学会学術総会　2014/09/25

  90. NUCB2 in human endometrial carcinoma as a potent prognostic factor

    Kiyoshi TAKAGI, Yasuhiro MIKI, Sota TANAKA, Nobuo YAEGASHI, Hironobu SASANO, Kiyoshi ITO, Takashi SUZUKI

    第73回日本癌学会学術総会　2014/09/25

  91. Transforming acidic coiled-coil-containing protein 2 (TACC2) immunolocalization in breast carcinoma

    Yoshiaki ONODERA, Kiyoshi TAKAGI, Yasuhiro MIKI, Yasuhiro NAKAMURA, Mika WATANABE, Takanori ISHIDA, Hironobu SASANO, Satoshi INOUE, Takashi SUZUKI

    第73回日本癌学会学術総会　2014/09/25

  92. 11β-Prostaglandin F2α produced by AKR1C3 stimulates FPreceptor and contributes to breast cancer progression

    Tomomi YODA, Keely M McNAMARA, Yasuhiro MIKI, Kiyoshi TAKAGI, Takashi SUZUKI, Hironobu SASANO

    第73回日本癌学会学術総会　2014/09/25

  93. ストレスホルモンと癌：子宮内膜癌におけるコルチゾール代謝

    三木康宏, 髙木清司, 鈴木 貴, 笹野公伸, 伊藤 潔

    第15回ホルモンと癌研究会　2014/07/04

  94. 乳癌微小環境において癌と脂肪細胞の相互作用に関与するS100A7の発現意義

    櫻井美奈子, 三木康宏, 髙木清司, 鈴木 貴, 石田孝宣, 大内憲明, 笹野公伸

    第15回ホルモンと癌研究会　2014/07/04

  95. 子宮内膜癌におけるNUCB2の発現意義意義

    高木清司, 三木康宏, 田中創太, 八重樫伸生, 笹野公伸, 伊藤 潔, 鈴木 貴

    第15回ホルモンと癌研究会　2014/07/04

  96. 類内膜型の子宮内膜癌における腫瘍組織中DHT濃度に関連した5αリダクターゼ1の意義

    田中創太, 三木康宏, 橋本千明, 高木清司, 鈴木 貴, 八重樫伸生, 伊藤 潔

    第15回ホルモンと癌研究会　2014/07/04

  97. ステロイドホルモン合成酵素AKR1C3は11β-Prostaglandin F2αの産生に寄与し,乳癌の悪化に関わる

    依田智美, McNamara M. Keely, 三木康宏, 髙木清司, 鈴木 貴, 石田孝宣, 大内憲明, 笹野公伸

    第15回ホルモンと癌研究会　2014/07/04

  98. 乳癌微小環境における27-hydroxy cholesterol合成酵素の発現

    石際康平, 櫻井美奈子, 三木康宏, 石田孝宣, 大内憲明, 笹野公伸

    第15回ホルモンと癌研究会　2014/07/04

  99. Intratumoral Concentration of Stress Hormone, Cortisol in Endometrial Carcinoma International-presentation

    Yasuhiro MIKI, Kiyoshi TAKAGI, Zhulanqiqige DOE, Sota TANAKA, Takashi SUZUKI, Hironobu SASANO, Kiyoshi ITO

    16th International Congress of Endocrinology/The 96th Annual Meeting of The Endocrine Society　2014/06/21

  100. Tumor Suppressive Roles of ARHGAP15 in Human Breast Cancer International-presentation

    Kiyoshi TAKAGI, Yasuhiro MIKI, Yoshiaki ONODERA, Yasuhiro NAKAMURA, Takanori ISHIDA, Hironobu SASANO, Takashi SUZUKI

    16th International Congress of Endocrinology/The 96th Annual Meeting of The Endocrine Society　2014/06/21

  101. Identification of Signaling Factors Involved in an Interaction Between Human Breast Carcinoma and Adipocytes International-presentation

    Minako SAKURAI, Yasuhiro MIKI, Kiyoshi TAKAGI, Takashi SUZUKI, Hironobu SASANO

    16th International Congress of Endocrinology/The 96th Annual Meeting of The Endocrine Society　2014/06/21

  102. 11β-Prostaglandin F2α Produced By AKR1C3 Stimulates FP-Receptor and Contributes to Breast Cancer Progression International-presentation

    Tomomi YODA, Keely McNAMARA, Yasuhiro MIKI, Takanori ISHIDA, Takashi SUZUKI, Noriaki OHUCHI, Hironobu SASANO

    16th International Congress of Endocrinology/The 96th Annual Meeting of The Endocrine Society　2014/06/21

  103. The Interaction of Androgen Pathways with the Pioneer Factor FOXA1 in Triple Negative Breast Cancers International-presentation

    Keely McNAMARA, Shota NAKAMURA, Tomomi YODA, Alif Meem NURANI, Yasuhiro MIKI, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    16th International Congress of Endocrinology/The 96th Annual Meeting of The Endocrine Society　2014/06/21

  104. 乳癌におけるheterogeneous nuclear ribonucleoprotein K の発現意義

    岩渕英里奈, 三木康宏, 増田真理子, 柴原裕紀子, 鈴木 貴, 笹野公伸

    第103回日本病理学会総会　2014/04/24

  105. mTOR 阻害剤による薬剤性肺障害の剖検例を含む肺組織におけるmTOR 活性の免疫組織学的解析

    齊藤涼子, 柳内 充, 三木康宏, 笠島敦子, 藤島史喜, 中村保宏, 海老名雅仁, 深澤雄一郎, 渡辺みか, 笹野公伸

    第103回日本病理学会総会　2014/04/24

  106. Estrogenic pathways exist in TNBC and correlate with androgen receptor status International-presentation

    Keely M McNAMARA, Tomomi YODA, Alif M NURANI, Yasuhiro MIKI, Takashi SUZUKI, Noriko NEMOTO, Minoru MIYASHITA, Kentaro TAMAKI, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    36th Annual San Antonio Breast Cancer Symposium　2013/12/10

  107. 浸潤性小葉癌におけるアンドロゲン代謝酵素の発現とその意義の解明

    依田智美, マクナマラ キーリー, 三木康宏, 高木まゆ, 雷 哲明, 平川 久, 石田孝宣, 鈴木 貴, 大内憲明, 笹野公伸

    第21回日本ステロイドホルモン学会　2013/11/16

  108. ヒト子宮内膜癌におけるCRH発現と予後の関連

    佐藤菜保子, 高木清司, 鈴木貴, 三木康宏, 割田仁, 福土審, 佐藤冨美子, 八重樫伸生, 伊藤 潔

    第51回日本癌治療学会学術集会　2013/10/24

  109. 閉経後非浸潤性乳管癌に対するレトロゾール術前内分泌療法の検討

    江幡明子, 石田孝宣, 高木清司, 三木康宏, 平川 久, 角川陽一郎, 天野吾郎, 森奈緒子, 中村保宏, 渡辺みか, 甘利正和, 笹野公伸, 鈴木 貴, 大内憲明

    第51回日本癌治療学会学術集会　2013/10/24

  110. Intratumoral androgen concentrations in endometrial carcinoma tissues International-presentation

    Sota TANAKA, Yasuhiro MIKI, Takashi SUZUKI, Zhulanqiqige Doe, Kiyoshi TAKAGI, Hiroki UTSUNOMIYA, Satoru NAGASE, Hitoshi NIIKURA, Nobuo YAEGASHI, Kiyoshi ITO

    The 18th International Meeting of the European Society of Gynaecological Oncology　2013/10/19

  111. Breast cancer, estrogen and obesity

    Hironobu SASANO, Minako SAKURAI, Yasuhiro MIKI

    第72回日本癌学会学術総会　2013/10/03

  112. Lin28B is a new potential molecular target in breast cancer progression, and enhanced by RNA binding protein, Musashi-1

    Minako SAKURAI, Yasuhiro MIKI, Yukiko SHIBAHARA, Takashi SUZUKI, Noriaki OHUCHI, Hironobu SASANO

    第72回日本癌学会学術総会　2013/10/03

  113. In situ detection of estrogen receptor dimers in breast carcinoma cells

    Erina IWABUCHI, Yasuhiro MIKI, Yoshiaki ONODERA, Katsuhiko ONO, Hironobu SASANO

    第72回日本癌学会学術総会　2013/10/03

  114. A Role for p62/SQSTM1 as a Crucial Mediator of Autophagy during Cancer Progression

    Kaito NIHIRA, Yasuhiro MIKI, Katsuhiko ONO, Takashi SUZUKI, Hironobu SASANO

    第72回日本癌学会学術総会　2013/10/03

  115. Immunolocalization of ARHGAP15 in human breast carcinoma

    Kiyoshi TAKAGI, Yasuhiro MIKI, Yoshiaki ONODERA, Yasuhiro NAKAMURA, Takanori ISHIDA, Hironobu SASANO, Takashi SUZUKI

    第72回日本癌学会学術総会　2013/10/03

  116. Novel Roles of Axl cleavage by its ligand Gas6 in non-small cell lung carcinoma

    Shinya IIDA, Yasuhiro MIKI, Yasuhiro NAKAMURA, Takashi SUZUKI, Hisafumi OKABE, Hironobu SASANO

    第72回日本癌学会学術総会　2013/10/03

  117. The effect of preoperative chemotherapy on ER, PR, HER2 and Ki-67 expression in metastatic lymph node of breast cancer

    Keiko UCHIDA, Yukiko SHIBAHARA, Yasuhiro MIKI, Shuko HATA, Erina IWABUCHI, Noriko NEMOTO, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    第72回日本癌学会学術総会　2013/10/03

  118. Androgenic Regulation of 17βHSD5 Expression in Molecular Apocrine Breast Cancer

    Tomomi YODA, Keely McNAMARA, Yasuhiro MIKI, Kiyoshi TAKAGI, Takashi SUZUKI, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    第72回日本癌学会学術総会　2013/10/03

  119. Immunolocalization of NRF2 in human breast carcinoma

    Yoshiaki ONODERA, Hozumi MOTOHASHI, Kiyoshi TAKAGI, Yasuhiro MIKI, Yukiko SHIBAHARA, Mika WATANABE, Takanori ISHIDA, Hironobu SASANO, Masayuki YAMAMOTO, Takashi SUZUKI

    第72回日本癌学会学術総会　2013/10/03

  120. Clinicopathological significance of Androgen Receptor status in recurrent triple negative breast carcinoma patients

    Keely M McNAMARA, Alif M NURANI, Tomomi YODA, Yasuhiro MIKI, Reiki NISHIMURA, Nobuyuki ARIMA, Takashi SUZUKI, Minoru MIYASHITA, Kentaro TAMAKI, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    第72回日本癌学会学術総会　2013/10/03

  121. オートファジー関連因子LC3A はEGFR チロシンキナーゼ阻害剤奏功性予測マーカーになり得る

    仁平開人, 三木康宏, 鳴海創大, 飯田慎也, 小野克彦, 海老名雅仁, 笹野公伸

    第33回日本分子腫瘍マーカー研究会　2013/10/02

  122. 近接ライゲーションアッセイ法を用いた乳癌培養細胞におけるエストロゲンレセプターダイマーの検出

    岩渕英里奈, 三木康宏, 小野寺好明, 小野克彦, 笹野公伸

    第54回日本組織細胞化学会総会・学術集会　2013/09/27

  123. Lack of androgen receptor (AR) associated with an increased Ki-67 labeling index in triple negative ductal carcinoma in situ International-presentation

    Keely M McNAMARA, Tomomi YODA, Alif Meem NURANI, Yasuhiro MIKI, Niramol CHANPLAKORN, Eriko ABE, Yang YANG, Koyu SUZUKI, Hisashi HIRAKAWA, Takashi SUZUKI, Noriko NEMOTO, Minoru MIYASHITA, Kentaro TAMAKI, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    The Annual Scientific Meeting of the Endocrine Society of Australia and the Society for Reproductive Biology　2013/08/25

  124. 口腔癌および前癌病変におけるEGFRとそのシグナル伝達分子の発現についての免疫組織化学的検討

    田代和樹, 渡邊純奈, 三木康宏, 及川麻理子, 清水良央, 熊本裕行

    第77回日本病理学会東北支部学術総会　2013/07/27

  125. 乳癌組織におけるARHGAP15の発現意義

    高木清司, 三木康宏, 小野寺好明, 中村保宏, 石田孝宣, 笹野公伸, 鈴木 貴

    第14回ホルモンと癌研究会　2013/07/12

  126. RNA結合タンパクMusashi-1は乳癌においてLIN28Bと協調的に進展を促進する

    櫻井美奈子, 三木康宏, 柴原裕紀子, 大内憲明, 鈴木 貴, 笹野公伸

    第14回ホルモンと癌研究会　2013/07/12

  127. 非浸潤性乳管癌 (DCIS) におけるアロマターゼ阻害剤の臨床病理的効果

    鈴木 貴, 高木清司, 三木康宏, 石田孝宣, 笹野公伸

    第14回ホルモンと癌研究会　2013/07/12

  128. 乳癌におけるステロイドホルモン代謝酵素17β-hydroxysteroid dehydrogenase type 5の発現調節

    依田智美, Keely May McNAMARA, 三木康宏, 高木清司, 鈴木 貴, 石田孝宣, 大内憲明, 笹野公伸

    第14回ホルモンと癌研究会　2013/07/12

  129. 浸潤性小葉癌と浸潤性乳管癌における臨床病理学的因子およびエストロゲン合成代謝酵素の病理学的比較検討

    高木まゆ, 石田孝宣, 甘利正和, 三木康宏, 玉城研太朗, 雷 哲明, 平川 久, 大内憲明, 笹野公伸

    第14回ホルモンと癌研究会　2013/07/12

  130. 浸潤性乳管癌と浸潤性小葉癌のホルモン代謝における病理学的検討比較

    第21回日本乳癌学会学術総会　2013/06/27

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    高木まゆ、石田孝宣、甘利正和、玉城研太朗、雷 哲明、平川 久、三木康宏、笹野公伸、大内憲明

  131. A comparison of an androgenic pathway between development and reoccurrence/progression of triple negative breast cancer patients International-presentation

    Keely M McNAMARA, Alif Meem NURANI, Tomomi YODA, Yasuhiro MIKI, Niramol CHANPLAKORN, Eriko ABE, Yang YANG, Hideko YAMAUCHI, Koyu SUZUKI, Hisashi HIRAKAWA, Reiki NISHIMURA, Nobuyuki ARIMA, Takashi SUZUKI, Minoru MIYASHITA, Kentaro TAMAKI, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    ENDO 2013 −The 95th Annual Meeting of The Endocrine Society−　2013/06/15

  132. Intratumoral Concentration of Stress Hormone Cortisol in Endometrial Carcinoma International-presentation

    Yasuhiro MIKI, Kiyoshi TAKAGI, Takashi SUZUKI, Kiyoshi ITO

    NIH-Tohoku Universtity-JSPS Symposium　2013/05/09

  133. Msi1 Enhances LIN28B Mediated Pathogenesis in Breast Cancer International-presentation

    Minako Sakurai, Yasuhiro Miki, Yukiko Shibara, Hisashi Hirakawa, Noriaki Ohuchi, Takashi Suzuki, Hironobu Sasano

    NIH-Tohoku Universtity-JSPS Symposium　2013/05/09

  134. ARHGAP15 in Human Breast Carcinoma International-presentation

    Kiyoshi TAKAGI, Yasuhiro MIKI, Yoshiaki ONODERA, Yasuhiko NAKAMURA, Takanori ISHIDA, Hironobu SASANO, Takashi SUZUKI

    NIH-Tohoku Universtity-JSPS Symposium　2013/05/09

  135. Androgenic Regulation of 17βHSD5 Expression in Breast Cancer International-presentation

    Tomomi YODA, Keely May McNAMARA, Yasuhiro MIKI, Kiyoshi TAKAGI, Takashi SUZUKI, Noriaki OHUCHI, Hironobu SASANO

    NIH-Tohoku Universtity-JSPS Symposium　2013/05/09

  136. 肺リンパ脈管筋腫症における性ステロイドホルモン受容体とホルモン合成酵素の発現

    安達厚子, 三木康宏, 斎藤涼子, 三上芳喜, 近藤 丘, 笹野公伸

    第86回日本内分泌学会学術総会　2013/04/25

  137. 肺動脈における肺高血圧症治療標的因子PDE5の発現とアンドロゲンによる発現調節

    安達厚子, 三木康宏, 斎藤涼子, 福本義弘, 下川宏明, 笹野公伸

    第86回日本内分泌学会学術総会　2013/04/25

  138. LIN28Bの乳癌における臨床病理学的意義

    櫻井美奈子, 三木康宏, 柴原裕紀子, 平川 久, 鈴木 貴, 大内憲明, 笹野公伸

    第86回日本内分泌学会学術総会　2013/04/25

  139. 乳癌におけるステロイドホルモン代謝酵素17β-hydroxysteroid dehydrogenase type 5の発現調節

    依田智美, McNamara Keely, 三木康宏, 高木清司, 鈴木 貴, 大内憲明, 笹野公伸

    第86回日本内分泌学会学術総会　2013/04/25

  140. 乳癌の転移リンパ節におけるアンドロゲンレセプターの発現解析

    柴原裕紀子, 櫻田千華子, 三木康宏, 笹野公伸

    第86回日本内分泌学会学術総会　2013/04/25

  141. Androgen metabolism in triple negative breast cancer (TNBC) patients -multivariate comparison of the findings

    マクナマラ キーリー, 依田智美, 三木康宏, チャンプラコン ニラモン, ヲングァイサヤワン サンサネエ, インチロエン ピンピン, コンダン ヨウワヌシュ, 王 リン, 高木清司, 高木まゆ, 中村保宏, 鈴木 貴, 宮下 穣, 根元紀子, 玉城研太郎, 石田孝宣, 大内憲明, 笹野公伸

    第86回日本内分泌学会学術総会　2013/04/25

  142. 浸潤性乳管癌と浸潤性小葉癌のエストロゲン合成代謝における病理学的検討比較

    高木まゆ, 石田孝宣, 甘利正和, 玉城研太郎, 雷 哲明, 平川 久, 三木康宏, 笹野公伸, 大内憲明

    第10回日本乳癌学会東北地方会　2013/03/02

  143. Endocrine disrupting effects of cigarette smoking International-presentation

    Yasuhiro MIKI, Takashi SUZUKI, Satoshi SUZUKI, Kiyoshi ITO, Hiroyuki KUMAMOTO, Hironobu SASANO

    15th International Congress on Hormonal Steroids and Hormones & Cancer　2012/11/15

  144. The roles of AR in triple negative breast cancer International-presentation

    Keely McNAMARA, Takashi SUZUKI, Yasuhiro MIKI, Hironobu SASANO

    15th International Congress on Hormonal Steroids and Hormones & Cancer　2012/11/15

  145. Endocrine disruptors and local estrogen production in the breast tumour microenvironment International-presentation

    Kevin C. KNOWER, Sarah Q. TO, Hoi Tin PUN, Kiyoshi TAKAGI, Yasuhiro MIKI, Hironobu SASANO, Evan R. SIMPSON, Wah Chin BOON, Colin D. CLYNE

    15th International Congress on Hormonal Steroids and Hormones & Cancer　2012/11/15

  146. ARHGAP15 in human breast carcinoma: potent favorable prognostic factor induced by androgen International-presentation

    Kiyoshi TAKAGI, Yasuhiro MIKI, Yoshiaki ONODERA, Yasuhiro NAKAMURA, Takanori ISHIDA, Hironobu SASANO, Takashi SUZUKI

    15th International Congress on Hormonal Steroids and Hormones & Cancer　2012/11/15

  147. LIN28B as a Potential Prognostic Marker of Breast Cancer Patients International-presentation

    Minako SAKURAI, Yasuhiro MIKI, Yukiko SHIBAHARA, Hisashi HIRAKAWA, Noriaki Ouchi, Takashi SUZUKI, Hironobu SASANO

    15th International Congress on Hormonal Steroids and Hormones & Cancer　2012/11/15

  148. Androgen receptor in invasive ductal carcinoma and ductal carcinoma in situ (DCIS) component in triple negative breast cancer (TNBC) patients International-presentation

    Keely McNAMARA, Tomomi YODA, Yasuhiro MIKI, Niramol CHANPLAKORN, Sansanee WONGWAISAYAWAN, Pimpin INCHAROEN, Youwanush KONGDAN, Yasuhiro NAKAMURA, Takashi SUZUKI, Noriko NEMOTO, Minoru MIYASHITA, Kentaro TAMAKI, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    15th International Congress on Hormonal Steroids and Hormones & Cancer　2012/11/15

  149. Androgenic Regulation of 17bHSD5 Expression in Breast Cancer International-presentation

    Tomomi YODA, Keely McNAMARA, Yasuhiro MIKI, Kiyoshi TAKAGI, Takashi SUZUKI, Noriaki Ohuchi, Hironobu SASANO

    15th International Congress on Hormonal Steroids and Hormones & Cancer　2012/11/15

  150. Aromatase inhibitor treatment of breast cancer cells increases the expression of let-7f, a microRNA targeting CYP19A1

    Yukiko SHIBAHARA, Yasuhiro MIKI, Shuko HATA, Yoshiaki ONODERA, Noriaki OHUCHI, Hironobu SASANO

    第71回日本癌学会学術総会　2012/09/19

  151. A Role for Macroautophagy in the Development of Lung Cancer Resistance to EGFR Tyrosine Kinase Inhibitor

    Kaito NIHIRA, Yasuhiro MIKI, Shinya IIDA, Katsuhiko ONO, Hironobu SASANO

    第71回日本癌学会学術総会　2012/09/19

  152. Activation of androgenic pathways results in lower tumor cell proliferation in triple negative breast cancer

    Kaito NIHIRA, Yasuhiro MIKI, Shinya IIDA, Katsuhiko ONO, Hironobu SASANO

    第71回日本癌学会学術総会　2012/09/19

  153. Expression of promyelocytic zinc finger protein (PLZF) in human breast carcinoma

    Kiyoshi TAKAGI, Yasuhiro MIKI, Yoshiaki ONODERA, Yasuhiro NAKAMURA, Takanori ISHIDA, Hironobu SASANO, Takashi SUZUKI

    第71回日本癌学会学術総会　2012/09/19

  154. Induction of aromatase through Aryl hydrocarbon receptor in ER-positive breast cancer

    Ryoko SAITO, Yasuhiro MIKI, Shinya IIDA, Shuko HATA, Kiyoshi TAKAGI, Katsuhiko ONO, Noriaki OHUCHI, Takashi SUZUKI, Hironobu SASANO

    第71回日本癌学会学術総会　2012/09/19

  155. 17-beta hydroxysteroid dehydrogenase type 1 in non-small cell lung carcinomas: a potential prognostic marker

    Mohit K VERMA, Yasuhiro MIKI, Takashi SUZUKI, Hironobu SASANO

    第71回日本癌学会学術総会　2012/09/19

  156. Ligand-dependent and independent activation of Axl in non-small cell lung carcinoma

    Shinya IIDA, Yasuhiro MIKI, Yasuhiro NAKAMURA, Takashi SUZUKI, Hisafumi OKABE, Hironobu SASANO

    第71回日本癌学会学術総会　2012/09/19

  157. Methylation and expression of the prostanoid receptor EP2 in ER+ breast cancer

    Sarah Q TO, Kiyoshi TAKAGI, Yasuhiro MIKI, Koyu SUZUKI, Eriko ABE, Yang YANG, Hironobu SASANO, Evan R SIMPSON, Colin D CLYNE, Kevin C KNOWER

    第71回日本癌学会学術総会　2012/09/19

  158. RUNX2 in human colon carcinoma: a potential prognostic factor associated with estrogen receptor

    Tomohiko SASE, Takashi SUZUKI, Kou MIURA, Kenichi SIIBA, Ikuro SATO, Kiyoshi TAKAGI, Yoshiaki ONODERA, Yasuhiro MIKI, Shinobu OHNUMA, Hiroyuki SASAKI, Chikashi SHIBATA, Michiaki UNNO, Hironobu SASANO

    第71回日本癌学会学術総会　2012/09/19

  159. 肺癌および乳癌における腫瘍マーカーとしてのp62/SQSTM1の意義

    仁平開人, 三木康宏, 鈴木 貴, 笹野公伸

    第32回日本分子腫瘍マーカー研究会　2012/09/18

  160. Expression of ARHGAP15 in human breast carcinoma International-presentation

    Kiyoshi TAKAGI, Yasuhiro MIKI, Yoshiaki ONODERA, Yasuhiro NAKAMURA, Takanori ISHIDA, Hironobu SASANO, Takashi SUZUKI

    14th International Congress of Histochemistry and Cytochemistry　2012/08/26

  161. In situ visualization of estrogen receptor dimers using Proximity Ligation Assay International-presentation

    Erina IWABUCHI, Yasuhiro MIKI, Yoshiaki ONODERA, Katsuhiko ONO, Hironobu SASANO

    14th International Congress of Histochemistry and Cytochemistry　2012/08/26

  162. タバコ煙抽出液による核内受容体の活性化

    三木康宏, 端 秀子, 熊本裕行, 笹野公伸

    第39回日本毒性学会学術年会　2012/07/17

  163. ヒト肝組織を用いた変異原性試験とCYPs発現プロファイルの関係

    端 秀子, 三木康宏, 笹野公伸

    第39回日本毒性学会学術年会　2012/07/17

  164. Cytochrome P450 19A1 (aromatase) in human liver and its diseases International-presentation

    Shuko HATA, Yasuhiro MIKI, Hironobu SASANO

    The 6th International Congress of Asian Society of Toxicology　2012/07/17

  165. Lung Cancer: An Estrogen Target? International-presentation

    Yasuhiro MIKI

    The 94th Annual Meeting of The Endocrine Society　2012/06/23

  166. Effects of Aromatase Inhibitor Therapy on Alteration of Enzymes Involved in Intratumoral Estrogen Production in Lung Carcinoma Cells International-presentation

    Mohit Kumar VERMA, Yasuhiro MIKI, Hironobu SASANO

    The 94th Annual Meeting of The Endocrine Society　2012/06/23

  167. Co-Expression of Androgen Receptor and 5αR1 in Triple-Negative Breast Cancer (TNBC) Is Associated with Lower Rates of Tumor Cell Proliferation International-presentation

    Keely May McNAMARA, Yasuhiro MIKI, Niramol CHANPLAKOM, Sansanee WONGWAISAYAWAN, Pimpin INCHAROEN, Yasuhiro NAKAMURA, Takashi SUZUKI, Minoru MIYASHITA, Kentaro TAMAKI, Takonori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    The 94th Annual Meeting of The Endocrine Society　2012/06/23

  168. Profiling of Nuclear Receptor Expression in Breast Cancer–Associated Fibroblasts Identifies RORA, THRB, VDR and PPARG as Potential Mediators within the Tumor Microenvironment International-presentation

    Kevin C KNOWER, Ashwini CHAND, Natalie ERIKSSON, Kiyoshi TAKAGI, Yasuhiro MIKI, Hironobu SASANO, Geoff LINDEMANN, Jane VISVADER, George MUSCAT, Evan R SIMPSON, Colin D CLYNE

    The 94th Annual Meeting of The Endocrine Society　2012/06/23

  169. 乳癌におけるアロマターゼ阻害剤投与によるmicroRNA の変動およびLet-7f によるCYP19A1の制御に関する検討

    柴原裕紀子, 三木康宏, 端 秀子, 小野寺好明, 中村保宏, 赤平純一, 阿部佳子, 笹野公伸

    第101回日本病理学会総会　2012/04/26

  170. エナメル上皮腫のおけるerbBファミリー分子に関する検討

    及川麻理子, 三木康宏, 清水良央, 熊本裕行

    第101回日本病理学会総会　2012/04/26

  171. 非小細胞肺癌におけるAxl およびGas6 の発現局在と意義

    飯田慎也, 三木康宏, 中村保宏, 鈴木 貴, 岡部尚文, 笹野公伸

    第101回日本病理学会総会　2012/04/26

  172. 乳癌組織におけるAryl hydrocarbon receptor と臨床学的事項およびaromatase 発現との関わりについての検討

    齊藤涼子, 三木康宏, 大庭悠貴, 端 秀子, 中村保宏, 鈴木 貴, 笹野公伸

    第101回日本病理学会総会　2012/04/26

  173. 近接ライゲーションアッセイ法を用いたestrogen receptor ダイマーの可視化の検討

    岩渕英里奈, 三木康宏, 小野寺好明, 小野克彦, 笹野公伸

    第101回日本病理学会総会　2012/04/26

  174. Proliferation and maturation of intratumoral blood vessels, the comparison between adenocarcinoma and squamous cell carcinoma

    Samaneh YAZDANI, Yasuhiro MIKI, Hironobu SASANO

    OOTR the 8th Annual Conference　2012/04/20

  175. 膵神経内分泌腫瘍における腫瘍増殖に関与する諸因子の免疫組織学的検討

    笠島敦子, 中村保宏, 三木康宏, 鈴木 貴, 笹野公伸

    第85回日本内分泌学会学術総会　2012/04/19

  176. 乳癌におけるpromyelocytic leukaemia zinc finger protein (PLZF)の発現意義

    高木清司, 三木康宏, 小野寺好明, 中村保宏, 笹野公伸, 鈴木 貴

    第12回特定非営利活動法人東北内分泌研究会総会　2012/04/07

  177. トリプルネガテイブ乳癌でのアンドロゲンによる腫瘍細胞の増殖低下作用の解析

    McNamara Keely, 三木康宏, Chanplakorn Niramol、Wongwaisayawan, Sansanee, Incharoen Pimpin, 中村保宏, 鈴木 貴, 宮下 穣, 玉城研太朗, 石田孝宣, 大内憲明, 笹野公伸

    第12回特定非営利活動法人東北内分泌研究会総会　2012/04/07

  178. ヒト乳癌組織におけるAryl hydrocarbon receptor (AhR)の発現の検討

    大庭悠貴, 齊藤涼子, 三木康宏, 飯田慎也, 端 秀子, 高木清司, 小野克彦, 鈴木 貴, 大内憲昭, 笹野公伸

    第12回特定非営利活動法人東北内分泌研究会総会　2012/04/07

  179. 骨芽細胞における乳癌治療薬（アロマターゼ阻害剤）の影響

    三木康宏, 端 秀子, 熊本裕行, 笹野公伸

    第33回東北骨代謝研究会　2012/02/04

  180. Metabotropic Glutamate Receptor 3 (GRM3) evaluation in Normal Adrenal Cortex, Adrenal Adenomas and Carcinoma

    フェリゾラ・サウロ, 中村保宏, 佐藤文俊, 小野寺好明, 岩渕絵里奈, 小野克彦, 三木康宏, 笹野公伸

    第15回日本内分泌病理学会　2011/11/23

  181. Aromatase inhibitor increases let-7f which targets CYP19A1 in Human Breast Cancer International-presentation

    Yukiko SHIBAHARA, Yasuhiro MIKI, Yoshiaki ONODERA, Shuko HATA, Hironobu SASANO

    Global breast cancer conference　2011/10/06

  182. Effects of smoking on intratumoral estrogen synthesis in lung cancer

    Yasuhiro MIKI, Takashi SUZUKI, Hiroyuki KUMAMOTO, Hironobu SASANO

    第70回日本癌学会学術総会　2011/10/03

  183. CEACAM family as a surrogate marker for tyrosine kinase inhibitors-sensitivity in human lung cancer

    Makoto KOBAYASHI, Yasuhiro MIKI, Keiko ABE, Katsuhiko ONO, Masahito EBINA, Ikuro SATO, Makoto MAEMONDO, Chiaki ENDO, Akira INOUE, Hisafumi OKABE, Takashi KONDO, Toshihiro NUKIWA, Hironobu SASANO

    第70回日本癌学会学術総会　2011/10/03

  184. Immunohistochemical analysis of Estrogen receptor α and β (ERα,ERβ)in human esophageal squamous cell carcinoma

    Masashi ZUGUCHI, Yasuhiro MIKI, Fumiyoshi FUJISHIMA, Go MIYATA, Susumu SATOMI, Hironobu SASANO

    第70回日本癌学会学術総会　2011/10/03

  185. KLF5 in human breast carcinoma

    Kiyoshi TAKAGI, Yasuhiro MIKI, Yoshiaki ONODERA, Yasuhiro NAKAMURA, Takanori ISHIDA, Satoshi INOUE, Hironobu SASANO, Takashi SUZUKI

    第70回日本癌学会学術総会　2011/10/03

  186. LIN28 regulates tumor suppressing activity of let-7 miRNA in human breast cancer

    Minako SAKURAI, Yasuhiro MIKI, Mariko MASUDA, Hisashi HIRAKAWA, Takashi SUZUKI, Hironobu SASANO

    第70回日本癌学会学術総会　2011/10/03

  187. Estrogen effects upon autophagic substrate p62 in non-small cell lung carcinomas

    Kaito NIHIRA, Shinya IIDA, Yasuhiro MIKI, Makoto KOBAYASHI, Hironobu SASANO

    第70回日本癌学会学術総会　2011/10/03

  188. Immunolocalization of amyloid precursor protein in human breast carcinoma

    Shigehiro ITO, Kiyoshi TAKAGI, Yoshiaki ONODERA, Yasuhiro MIKI, Yasuhiro NAKAMURA, Takanori ISHIDA, Satoshi INOUE, Hironobu SASANO, Takashi SUZUKI

    第70回日本癌学会学術総会　2011/10/03

  189. 乳癌組織におけるAmyloid Precursor Protein (APP) の発現意義

    伊藤重広, 高木清司, 小野寺好明, 三木康宏, 中村保宏, 石田孝宣, 井上 聡, 笹野公伸, 鈴木 貴

    第11回特定非営利活動法人東北内分泌研究会総会　2011/10/01

  190. Metabotropic Glutamate Receptor 3 (GRM3) evaluation in Normal Adrenal Cortex, Adrenal Adenomas and Carcinoma

    SAURO J.A. FELIZOLA, 中村保宏, 佐藤文俊, 小野寺好明, 岩渕絵里奈, 小野克彦, 三木康宏, 笹野公伸

    第11回特定非営利活動法人東北内分泌研究会総会　2011/10/01

  191. 乳癌組織におけるaromatase 発現調節機構の解明

    三木康宏

    第19回日本乳癌学会学術総会　2011/09/02

  192. 乳癌におけるaryl hydrocarbon receptor と腫瘍内Estrogen 産生との関係

    斎藤涼子, 三木康宏, 端 秀子, 飯田慎也, 小野克彦, 石田孝宣, 鈴木 貴, 大内憲明, 笹野公伸

    第19回日本乳癌学会学術総会　2011/09/02

  193. 非浸潤性及び浸潤性乳癌組織におけるエストロゲン作用

    江幡明子, 鈴木 貴, 高木清司, 三木康宏, 五十嵐やよい, 中村保宏, 石田和之, 渡辺みか, 石田孝宣, 大内憲明, 笹野公伸

    第19回日本乳癌学会学術総会　2011/09/02

  194. 男子乳癌におけるエストロゲン作用

    高木清司, 森谷卓也, 黒住昌史, 岡きま子, 豊島 隆, 武井寛幸, 平川 久, 石田孝宣, 紅林淳一, 林 慎一, 三木康宏, 笹野公伸, 鈴木 貴

    第19回日本乳癌学会学術総会　2011/09/02

  195. 乳癌原発巣および転移リンパ節でのアロマターゼ免疫染色の比較検討

    柴原裕紀子, 三木康宏, 工藤陽花子, 小野克彦, 石田孝宣, 笹野公伸

    第19回日本乳癌学会学術総会　2011/09/02

  196. ヒトER 陽性乳癌におけるエストロゲン誘導性microRNA-7 とEGFR 発現

    増田真理子, 三木康宏, 端 秀子, 鈴木 貴, 石田孝宣, 平川 久, 大内憲明, 笹野公伸

    第19回日本乳癌学会学術総会　2011/09/02

  197. ヒト乳癌におけるSuppressor of Cytokine Signalling 3（SOCS3）発現の意義

    櫻井美奈子, 三木康宏, 石田孝宣, 鈴木 貴, 大内憲明, 笹野公伸

    第19回日本乳癌学会学術総会　2011/09/02

  198. KLF5 in human breast carcinoma

    Kiyoshi TAKAGI, Yasuhiro MIKI, Yoshiaki ONODERA, Yasuhiro NAKAMURA, Takanori ISHIDA, Satoshi INOUE, Hironobu SASANO, Takashi SUZUKI

    第70回日本癌学会学術総会　2011/09/02

  199. ヒト乳癌におけるLIN28によるlet-7発現抑制

    櫻井美奈子, 三木康宏, 増田真理子, 柴原裕紀子, 平川久, 鈴木 貴, 笹野公伸

    第12回ホルモンと癌研究会　2011/07/15

  200. 乳癌におけるアンドロゲン応答遺伝子の多様性

    高木清司, 三木康宏, 小野寺好明, 中村保宏, 石田孝宣, 笹野公伸, 鈴木 貴

    第12回ホルモンと癌研究会　2011/07/15

  201. 骨と性ホルモン

    三木康宏, 熊本裕行, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  202. タバコ煙抽出液の核内受容体リガンド活性

    三木康宏, 端 秀子, 鈴木 貴, 熊本裕行, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  203. エストロゲン陽性乳癌における原発巣と転移リンパ節のアロマターゼ比較検討

    柴原裕紀子, 三木康宏, 小野克彦, 工藤陽花子, 中村保宏, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  204. 乳癌におけるSuppressor of Cytokine Signaling (SOCS3) の発現意義

    櫻井美奈子, 三木康宏, 石田孝宣, 鈴木 貴, 大内憲明, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  205. Cytoplasmic estrogen receptor b as a potential prognosis marker in human non-small cell lung carcinoma

    Mohit Kumar Verma, Yasuhiro MIKI, Keiko Abe, Hiromichi Niikawa, Hironobu SASANO

    第29回内分泌代謝学サマーセミナー　2011/07/07

  206. ヒト皮脂腺におけるステロイドホルモン関連因子の発現解析

    井上高良, 三木康宏, 角尾進吾, 八谷 輝, 北原 隆, 相場節也, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  207. 神経内分泌腫瘍に対する分子標的治療薬の効果と臨床応用への可能性

    飯田慎也, 三木康宏, 小野克彦, 中村保宏, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  208. ヒト転移性肝癌におけるaromatase の発現

    端 秀子, 三木康宏, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  209. 乳癌における腫瘍内Estrogen産生とarylhydrocarbon receptorの関連

    齊藤涼子, 三木康宏, 端 秀子, 飯田慎也, 小野克彦, 石田孝宣, 鈴木 貴, 大内憲明, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  210. 乳癌組織におけるアミロイド前駆体タンパクの発現

    伊藤重広, 高木清司, 小野寺好明, 三木康宏, 中村保宏, 石田孝宣, 井上 聡, 笹野公伸, 鈴木 貴

    第29回内分泌代謝学サマーセミナー　2011/07/07

  211. 正常副腎皮質、副腎腫瘍における代謝型グルタミン酸受容体３型（GRM3）の発現解析

    フェリゾラ・サウロ, 中村保宏, 佐藤文俊, 小野寺好明, 岩渕絵里奈, 小野克彦, 三木康宏, 笹野公伸

    第29回内分泌代謝学サマーセミナー　2011/07/07

  212. 口腔扁平上皮癌における多剤耐性因子の発現

    三木康宏, 及川麻理子, 小野克彦, 清水良央, 安藤紀昭, 笹野公伸, 熊本裕行

    第100回日本病理学会総会　2011/04/28

  213. 乳癌における原発巣および転移リンパ節のアロマターゼ免疫染色の比較検討

    柴原裕紀子, 三木康宏, 阿部佳子, 笹野公伸

    第100回日本病理学会総会　2011/04/28

  214. 非小細胞肺癌組織におけるAxl の活性化と発現意義

    飯田 慎也, 三木康宏, 阿部佳子, 小野克彦, 中村保宏, 鈴木 貴, 岡部尚文, 笹野公伸

    第100回日本病理学会総会　2011/04/28

  215. 間質性肺炎におけるエストロゲン受容体とアロマターゼの発現

    谷内真司, 鈴木 貴, 三木康宏, 阿部佳子, 藤島史喜, 中村保宏, 佐藤聡子, 笠島敦子, 石田和之, 渡邊みか, 笹野公伸

    第100回日本病理学会総会　2011/04/28

  216. 免疫組織染色に適したメラニン含有細胞の識別法の探索

    森 美紀, 藤島史喜, 三木康宏, 阿部佳子, 岩渕英里奈, 三浦博守, 渡邊みか, 笹野公伸

    第100回日本病理学会総会　2011/04/28

  217. 骨芽細胞におけるaryl hydrocarbon receptorを介したエストロゲン合成

    三木康宏, 鈴木 貴, 熊本裕行, 笹野公伸

    第84回日本内分泌学会学術総会　2011/04/21

  218. 神経内分泌腫瘍組織におけるinsulin-like growth factor-1 receptor （IGF-1R） の活性化と細胞内シグナル伝達系との関連性

    飯田慎也, 三木康宏, 小野克彦, 中村保宏, 鈴木 貴, 笹野公伸

    第84回日本内分泌学会学術総会　2011/04/21

  219. ヒト皮膚における性ステロイド濃度の解明と、aromtase、steroidogenic acute regulatory proteinの役割

    井上高良, 三木康宏, 阿部佳子, 羽鳥正仁, 保坂正美, 角尾進吾, 藤村 努, 八谷 輝, 北原 隆, 本間誠二郎, 相場節也, 笹野公伸

    第84回日本内分泌学会学術総会　2011/04/21

  220. Suppressor of cytokine signalling-3 (SOCS3) in human breast carcinoma International-presentation

    Minako SAKURAI, Yasuhiro MIKI, Hironobu SASANO

    Congress on Steroid Research 2011　2011/03/27

  221. Aryl hydrocarbon receptor regulates estrogen synthesis in osteoblast cells

    Yasuhiro MIKI, Shuko HATA, Katsuhiko ONO, Hironobu SASANO, Hiroyuki KUMAMOTO

    The 4th International Symposium for Interface Oral Health Science　2011/03/07

  222. 骨芽細胞におけるaryl hydrocarbon receptorのエストロゲン合成への関与

    三木康宏, 端 秀子, 鈴木 貴, 熊本裕行, 笹野公伸

    第32回 東北骨代謝研究会　2011/02/05

  223. 転移性肝癌におけるaromataseの発現

    端 秀子, 三木康宏, 鈴木 貴, 笹野公伸

    第9回特定非営利活動法人東北内分泌研究会総会　2010/10/02

  224. Aromatase expression in lung carcinoma cells through intratumoral stromal cell interactions（肺癌におけるアロマターゼ発現に対する癌間質細胞の影響）

    Yasuhiro MIKI, Takashi SUZUKI, Keiko ABE, Shinya IIDA, Jun-ichi AKAHIRA, Hironobu SASANO

    第69回日本癌学会学術総会　2010/09/22

  225. Individual differences in human CYPs: correlation between mutagenicty test and gene expression profiles（ヒト肝組織におけるCYPsの個人差：変異原性試験と遺伝子発現プロファイルの関係）

    Shuko HATA, Yasuhiro MIKI, Jun-ichi AKAHIRA, Michiaki Unno, Hironobu SASANO

    第69回日本癌学会学術総会　2010/09/22

  226. MiRNA profile following aromatase inhibitor in breast cancer-potential difference between steroidal/non-steroidal agents（ヒト乳癌におけるアロマターゼ阻害薬によるmiRNA発現の変動）

    Yukiko Shibahara, Yasuhiro MIKI, Katsuhiko ONO, Jun-ichi AKAHIRA, Keiko ABE, Takashi SUZUKI, Hironobu SASANO

    第69回日本癌学会学術総会　2010/09/22

  227. Axl asa a key factor for cell signal non-small cell lung carcinoma cells（非小細胞肺癌細胞の細胞増殖シグナルにおけるAxlの役割）

    Shinya IIDA, Yasuhiro MIKI, Katsuhiko ONO, Keiko ABE, Jun-ichi AKAHIRA, Takashi SUZUKI, Hisafumi OKABE, Hironobu SASANO

    第69回日本癌学会学術総会　2010/09/22

  228. Estrogen receptor beta and aromatase in lung cancer: co-expression associated with gender dependent clinical outcome

    Mohit K. Verma, Yasuhiro MIKI, Keiko ABE, Hiromichi NIIKAWA, Hironobu SASANO

    第69回日本癌学会学術総会　2010/09/22

  229. Estrogen-dependent expression of miRNAs in human breast cancer（ヒト乳癌におけるエストロゲン依存性miRNAの発現）

    Mariko Masuda, Shuko HATA, Yasuhiro MIKI, Hironobu SASANO

    第69回日本癌学会学術総会　2010/09/22

  230. Expression of estrogen-related enzymes in benign breast disease: comparison with normal breast and breast carcinoma（乳腺良性疾患におけるエストロゲン合成代謝酵素の発現の検討）

    Yoshie SASAKI, Yoshiaki ONODERA, Yasuhiro MIKI, Jun-ichi AKAHIRA, Hironobu SASANO, Takashi SUZUKI

    第69回日本癌学会学術総会　2010/09/22

  231. Expression of NUCB2 and its significance in human breast carcinoma（乳癌におけるNUCB2の発現意義）

    Shiho SUZUKI, Yoshiaki ONODERA, Yasuhiro MIKI, Jun-ichi AKAHIRA, Hironobu SASANO, Takashi SUZUKI

    第69回日本癌学会学術総会　2010/09/22

  232. Runx2 in human breast carcinoma-its potential roles in cancer progression（乳癌におけるRunx2—その進行における役割について）

    Yoshiaki ONODERA, Takashi SUZUKI, Kiyoshi TAKAGI, Yasuhiro MIKI, Jun-ichi AKAHIRA, Hironobu SASANO

    第69回日本癌学会学術総会　2010/09/22

  233. Sex-steroid receptors in male breast carcinoma（男子乳癌における性ホルモン受容体の発現）

    Kimako OKA, Takuya MORIYA, Masafumi KUROSUMI, Kiyoshi TAKAGI, Yasuhiro MIKI, Takashi TOYOHSIMA, Hiroyuki TAKEI, Hisashi HIRAKAWA, Takanori ISHIDA, Junichi KUREBAYASHI, Shin-ichi HAYASHI, Hironobu SASANO, Takashi SUZUKI

    第69回日本癌学会学術総会　2010/09/22

  234. Aromatase in the breast carcinoma as a negative regulator of intratumoral androgen production（アロマターゼによる乳癌組織におけるアンドロゲン合成の抑制）

    Kiyoshi TAKAGI, Yasuhiro MIKI, Noriaki OHUCHI, Hironobu SASANO, Takashi SUZUKI

    第69回日本癌学会学術総会　2010/09/22

  235. 肺癌における性ステロイドホルモンの作用

    三木康宏, 阿部佳子, 鈴木 貴, 笹野公伸

    第51回日本組織細胞化学会総会・学術集会　2010/09/04

  236. 乳癌におけるアロマターゼの発現意義：アンドロゲン局所合成の観点から

    鈴木 貴, 高木清司, 三木康宏, 大内憲明, 笹野公伸

    第11回ホルモンと癌研究会　2010/07/30

  237. Potential combination of mTOR and MEK inhibitors and their synergic therapeutic effects in non-functioning neuroendocrine tumor cell lines

    Shinya IIDA, Yasuhiro MIKI, Katsuhiko ONO, Jun-ichi AKAHIRA, Takashi SUZUKI, Hironobu SASANO

    第28回内分泌代謝学サマーセミナー　2010/07/08

  238. 皮膚扁平上皮癌および皮膚上皮内癌におけるエストロゲン受容体とアロマターゼの発現の検討

    森 美紀, 阿部佳子, 小野克彦, 三木康宏, 赤平純一, 笹野公伸

    第99回日本病理学会総会　2010/04/27

  239. 間質性肺炎におけるエストロゲン受容体とアロマターゼの発現

    谷内真司, 鈴木 貴, 三木康宏, 阿部佳子, 鈴木瑛介, 中村保宏, 藤島史喜, 伊藤しげみ, 石田和之, 渡辺みか, 笹野公伸

    第99回日本病理学会総会　2010/04/27

  240. 皮膚扁平上皮癌および皮膚上皮内癌におけるエストロゲン受容体とアロマターゼ

    森 美紀, 阿部佳子, 小野克彦, 三木康宏, 赤平純一, 笹野公伸

    第99回日本病理学会総会　2010/04/27

  241. 乳癌におけるNUCB2の発現意義の検討

    鈴木志穂, 小野寺好明, 三木康宏, 赤平純一, 笹野公伸, 鈴木 貴

    第8回特定非営利活動法人東北内分泌研究会総会　2010/04/24

  242. 乳癌組織におけるアンドロゲン受容体及びアンドロゲン関連遺伝子IQWD-1の検討

    菅原裕人, 中野基一郎, 三木康宏, 赤平純一, 鈴木 貴, 笹野公伸

    第8回特定非営利活動法人東北内分泌研究会総会　2010/04/24

  243. 乳腺良性疾患におけるエストロゲン合成代謝酵素の発現の検討

    佐々木淑恵, 小野寺好明, 三木康宏, 赤平純一, 笹野公伸, 鈴木 貴

    第8回特定非営利活動法人東北内分泌研究会総会　2010/04/24

  244. 乳癌におけるエストロゲン誘発性microRNAの解析

    増田真理子, 端 秀子, 三木康宏, 笹野公伸

    第8回特定非営利活動法人東北内分泌研究会総会　2010/04/24

  245. 皮膚扁平上皮癌におけるエストロゲン受容体とアロマターゼの発現の検討

    森 美紀, 阿部佳子, 小野克彦, 三木康宏, 赤平純一, 笹野公伸

    第8回特定非営利活動法人東北内分泌研究会総会　2010/04/24

  246. Immunolocalization of estrogen-related enzymes in genign human breast disorders International-presentation

    Yoshie Sasaki, Yosiaki Onodera, Yasuhiro Miki, Jun-ichi Akahira, Hironobu Sasano, Takashi Suzuki

    14th International Congress of Endocrinology　2010/03/26

  247. Estrogen receptors in human skin-its correlation with age and gender- International-presentation

    Yasuhiro Miki, Keiko Abe, Miki Mori, Yosiyuki Kariya, Setsuya Aiba, Hironobu Sasano

    14th International Congress of Endocrinology　2010/03/26

  248. Expression of mTOR signaling pathway and effects of mTOR inhibitor on cell signaling pathway in neuroendocrine tumors International-presentation

    Shinya Iida, Tasuhiro Miki, Katsuhiro Ono, Hironobu Sasano

    14th International Congress of Endocrinology　2010/03/26

  249. Immunolocalization of NUCB2 in human breast carcinoma International-presentation

    Shiho Suzuki, Yoshiaki Onodera, Yasuhiro Miki, Jun-ichi Akahira, Hironobu Sasano, Takashi Suzuki

    14th International Congress of Endocrinology　2010/03/26

  250. Estrogen receptor βin human non-small cell lung carcinoma:Cytoplasmic ER β as a potential prognosis marker International-presentation

    Mohito K.Verma, Yasuhiro Miki, Keiko Abe, Hiromichi Niikawa, Hironobu Sasano

    14th International Congress of Endocrinology　2010/03/26

  251. Identification of estradiol-estrogen receptor-regulated miRNAs in breast cancer cells International-presentation

    Mariko Masuda, Syuko Hata, Yasuhiro Miki, Hironobu Sasano

    14th International Congress of Endocrinology　2010/03/26

  252. ntratumoral androgen levels brest carcinoma with aromatase inhibitor exemestane:17β-hydroxysteroid dehydrogenase type 2 as a maker for increased androgen actions International-presentation

    Kiyosi Takagi, Shuji Nagasaki, Yoshiaki Onodera, Yasuhiro Miki, Jun-ichi Akahira, Katsuhiko Ono, Hisashi Hirakawa, Hironobu Sasano

    14th International Congress of Endocrinology　2010/03/26

  253. Tissue concentration of sex steroid hormones and its correlations with clinicopathological parameters in human epithelial ovarian cancer International-presentation

    Jun-ichi Akahira, Fumihiro Suzuki, TakashiSuzuki, Yasuhiro Miki, Shuji Nagasaki, Seijiro Honma, Kiyoshi Ito, Nobuo Yaegashi, Hironobu Sasano

    14th International Congress of Endocrinology　2010/03/26

  254. エストロゲン環境の撹乱を介した喫煙の肺癌プログレッション作用

    三木康宏, 鈴木 貴, 阿部佳子, 鈴木 聡, 笹野公伸

    第83回日本内分泌学会学術総会　2010/03/25

  255. Estrogen receptor and aromatase in squamous cell carcinoma of the skin International-presentation

    Keiko ABE, Miki MORI, Yasuhiro MIKI, Takayoshi INOUE, Katsuhiko ONO, Yukiko SHIBAHARA, Jun-ichi AKAHIRA, Hironobu SASANO

    99th Annual Meeting of United States and Canadian Academy of Pathology　2010/03/20

  256. Increased estrogen sulfatase (STS) and 17β- hydroxysteroid dehydrogenase type 1(17β-HSD1) after neoadjuvant aromatase inhibitor therapy in breast cancer patients International-presentation

    Niramol CHANPLAKORN, Pongsthorn CHANPLAKORN, Katsuhiko ONO, Yasuhiro MIKI, Shigetoyo SAJI, Takayuki UENO, Masakazu TOI, Hironobu SASANO

    OOTR the 6th Annual Conference　2010/02/26

  257. 神経内分泌腫瘍組織における標的分子の発現解析とmTOR阻害剤およびMEK阻害剤の併用効果に関する検討

    飯田慎也, 三木康宏, 小野克彦, 笹野公伸

    第15回 NET Work Japan　2010/01/30

  258. Intratumoral estrogen production in human lung carcinoma

    Suzuki T, Miki Y, Sasano H

    4th International Congress on Hormonal Steroids and Steroids & Cancer (Symposium)　2010

  259. Human lung cancer microenvironment： Effects of intratumoral stromal cells on cell proliferation of carcinoma cells.

    2009/10

  260. Expression of estrogen receptors and aromatase in Japanese female patients with pulmonary adenocarcinoma: A comparioson between BAC and non-BAC International-presentation

    Keiko ABE, Yasuhiro MIKI, Katsuhiko ONO, Naohiko KIKUCHI, Satoshi SUZUKI, Toru TAKAHASHI, Hironobu SASANO

    22nd European Congress of Pathology　2009/09/04

  261. 肺癌組織におけるエストロゲン合成と喫煙の関係

    三木康宏, 鈴木 聡, 阿部佳子, 鈴木 貴, 笹野公伸

    第12回伊豆レスピロロジーフォーラム　2009/08/22

  262. 肺癌におけるaromataseの発現とその誘導因子の検討

    三木康宏, 阿部佳子, 鈴木 貴, 鈴木 聡, 赤平純一, 笹野公伸

    第10回ホルモンと癌研究会　2009/07/31

  263. アロマターゼ阻害剤投与に伴う乳癌組織中アンドロゲン濃度の変化

    高木清司, 小野寺好明, 三木康宏, 小野克彦, 平川 久, 笹野公伸, 鈴木 貴

    第10回ホルモンと癌研究会　2009/07/31

  264. ヒト骨組織におけるaryl hydrocarbon receptorの発現

    三木康宏, 端 秀子, 長崎修治, 鈴木 貴, 笹野公伸

    第36回日本トキシコロジー学会学術年会　2009/07/06

  265. ヒト肝組織における薬物代謝の個人差―Ames試験と遺伝子発現プロファイルの関連

    端 秀子, 三木康宏, 長崎修治, 笹野公伸

    第36回日本トキシコロジー学会学術年会　2009/07/06

  266. 臨床的にDiGeorge症候群が疑われたEBV陽性T細胞性悪性リンパ腫の１剖検例

    伊藤しげみ, 三木康宏, 笹野公伸, 一迫 玲

    第98回日本病理学会総会　2009/05/01

  267. 皮膚扁平上皮癌におけるホルモン受容体とアロマターゼの発現の検討

    森 美紀, 阿部佳子, 小野克彦, 三木康宏, 赤平純一, 笹野公伸

    第98回日本病理学会総会　2009/05/01

  268. 10％ホルマリン固定パラフィン包埋標本から抽出したTotal RNAおよびmicroRNAの質的検討

    三浦伊久美, 三木康宏, 赤平純一, 鈴木 貴, 笹野公伸

    第98回日本病理学会総会　2009/05/01

  269. 卵巣癌におけるsteroid and xenobiotic receptorの発現と臨床病理学的因子との関連についての検討

    楽 暁妮, 赤平純一, 三木康宏, 宇都宮裕貴, 伊藤 潔, 笹野公伸, 八重樫伸生

    第98回日本病理学会総会　2009/05/01

  270. ヒト肺癌におけるsteroid sulfataseの発現

    三木康宏, 柿沼秀明, 阿部佳子, 鈴木 聡, 赤平純一, 鈴木 貴, 笹野公伸

    第82回日本内分泌学会学術総会　2009/04/23

  271. 卵巣癌におけるsteroid and xenobiotic receptorの発現と臨床病理学的因子との関連についての検討

    楽 暁ジ, 赤平純一, 三木康宏, 宇都宮裕貴, 伊藤 潔, 笹野公伸, 八重樫伸生

    第82回日本内分泌学会学術総会　2009/04/23

  272. 乳癌における17beta-hydroxysteroid dehydrogenase type12の機能に関する検討

    長崎修治, 鈴木 貴, 三木康宏, 赤平純一, 笹野公伸

    第82回日本内分泌学会学術総会　2009/04/23

  273. 神経内分泌腫瘍に対するmTOR阻害剤の影響とソマトスタチン誘導体の併用効果に関する検討

    飯田慎也, 三木康宏, 小野克彦, 笹野公伸

    第82回日本内分泌学会学術総会　2009/04/23

  274. 神経内分泌腫瘍に対する新規併用薬物療法に関する基礎的検討

    飯田慎也, 三木康宏, 小野克彦, 笹野公伸

    第6 回特定非営利活動法人東北内分泌研究会総会（第18 回日本内分泌学会東北地方会）　2009/04/04

  275. 乳癌に対するアロマターゼ阻害薬—microRNAの関与

    柴原裕紀子, 三木康宏, 小野克彦, 赤平純一, 鈴木 貴, 笹野公伸

    第6 回特定非営利活動法人東北内分泌研究会総会（第18 回日本内分泌学会東北地方会）　2009/04/04

  276. 正常皮膚組織におけるエストロゲン関連因子の発現解析

    井上高良, 三木康宏, 小野克彦, 森 美紀, 笹野公伸

    第6 回特定非営利活動法人東北内分泌研究会総会（第18 回日本内分泌学会東北地方会）　2009/04/04

  277. 乳癌組織におけるexemestane投与に伴う性ホルモン合成／代謝酵素の発現変動

    高木清司, 長崎修治, 小野寺好明, 三木康宏, 小野克彦, 平川 久, 笹野公伸, 鈴木 貴

    第6 回特定非営利活動法人東北内分泌研究会総会（第18 回日本内分泌学会東北地方会）　2009/04/04

  278. 女性肺腺癌組織におけるステロイドホルモン受容体およびアロマターゼ発現の免疫組織化学的検討

    菊池直彦, 阿部佳子, 三木康宏, 小野克彦, 鈴木 聡, 高橋 徹, 笹野公伸

    第6 回特定非営利活動法人東北内分泌研究会総会（第18 回日本内分泌学会東北地方会）　2009/04/04

  279. ヒト肝組織を用いたAmes試験と薬物代謝酵素の個人差の解析

    端 秀子, 三木康宏, 笹野公伸

    日本農芸化学会2009年度大会　2009/03/27

  280. 肺癌におけるaromataseの発現と喫煙の影響

    三木康宏, 新井川弘道, 鈴木 聡, 端 秀子, 阿部佳子, 鈴木 貴, 笹野公伸

    第17回バイオフィジオロジー研究会　2009/03/06

  281. Sex steroid receptors expression and hormone-induced cell proliferation in human osteosarcoma International-presentation

    Osamu DOHI, Masahito HATORI, Takashi SUZUKI, Katsuhiko ONO, Masami HOSAKA, Jun-ichi AKAHIRA, Yasuhiro MIKI, Shuji NAGASAKI, Eiji ITOI, Hironobu SASANO

    55th Annual Meeting of the Orthopaedic Research Society　2009/02/22

  282. ヒト骨芽細胞における内分泌撹乱物質の影響：Steroid and xenobiotic receptorを介したbisphenol Aの作用

    三木康宏, 端 秀子, 笹野公伸

    第30回 東北骨代謝研究会　2009/02/07

  283. 神経内分泌腫瘍組織における活性化mTORの発現とmTOR阻害剤およびソマトスタチン誘導体の単独・併用効果に関する検討

    飯田慎也, 三木康宏, 小野克彦, 笹野公伸

    第4回 NET Work Japan　2009/01/24

  284. 乳癌におけるCOUP-TFIIの発現解析

    長崎修治, 鈴木 貴, 三木康宏, 赤平純一, 笹野公伸

    第16回日本ステロイドホルモン学会学術総会　2008/11/22

  285. 肺癌組織における微小環境：癌細胞増殖における間質細胞の影響

    三木康宏, 新井川弘道, 森 一茂, 鈴木 貴, 赤平純一, 鈴木 聡, 岡部尚文, 近藤 丘, 笹野公伸

    第46回 日本癌治療学会総会　2008/10/30

  286. Aromatase as a possible target for human lung cancer therapy

    Yasuhiro Miki, Takashi Suzuki, Jun-ichi Akahira, Keiko Abe, Hironobu Sasano

    第67回日本癌学会学術総会　2008/10/28

  287. 乳癌における17β-hydroxysteroid dehydrogenase type12 の機能に関する検討

    長崎修治, 鈴木 貴, 三木康宏, 赤平純一, 笹野公伸

    第67回日本癌学会学術総会　2008/10/28

  288. 上皮性卵巣癌における組織中性ステロイドホルモン濃度と臨床病理学的因子の関連について

    赤平純一, 鈴木史彦, 鈴木 貴, 伊藤 潔, 三木康宏, 長崎修治, 八重樫伸生, 笹野公伸

    第67回日本癌学会学術総会　2008/10/28

  289. 大腸癌におけるステロイドサルファターゼとエストロゲンスルフォトランスフェラーゼの発現意義

    佐藤龍一郎, 鈴木 貴, 片寄 友, 三浦 康, 椎葉健一, 立野紘雄, 三木康宏, 赤平純一, 山本久仁治, 井伊貴幸, 江川新一, 海野倫明, 笹野公伸

    第67回日本癌学会学術総会　2008/10/28

  290. 乳癌におけるNUDT2の発現に関する免疫組織化学的検討

    岡きま子, 鈴木 貴, 赤平純一, 三木康宏, 小野寺好明, 小野克彦, 長崎修治, 森 美紀, 石田孝宣, 大内憲明, 笹野公伸

    第67回日本癌学会学術総会　2008/10/28

  291. ヒト肝組織を用いたAmes試験とCYPsの発現プロファイルの個人差の比較

    端 秀子, 三木康宏, 笹野公伸

    フォーラム2008：衛生薬学・環境トキシコロジー　2008/10/17

  292. ESTROGEN RECEPTOR β IN NON-SMALL CELL LUNG CANCER – a significant correlation with intratumoral aromatase- International-presentation

    Keiko ABE, Yasuhiro MIKI, Miki MORI, Yuki KOU, Nobutaka KUDO, Masashi KOGUCHI, Hironobu SASANO

    27th Congress of the International Academy of Pathology　2008/10/12

  293. 肺癌におけるEstrogen合成酵素の発現

    柿沼秀明, 三木康宏, 笹野公伸

    第5 回特定非営利活動法人東北内分泌研究会総会　2008/10/04

  294. 神経内分泌腫瘍に対するmTOR阻害剤の適応と基礎的検討

    飯田慎也, 小野克彦, 三木康宏, 笹野公伸

    第5 回特定非営利活動法人東北内分泌研究会総会　2008/10/04

  295. 乳癌におけるCOUP-TF IIの免疫組織化学的検討

    長崎修治, 鈴木 貴, 三木康宏, 赤平純一, 笹野公伸

    第5 回特定非営利活動法人東北内分泌研究会総会　2008/10/04

  296. Proliferative effect of estrogen on human lung cancer in vitro -Aromatase as a possible target for human lung cancer therapy- International-presentation

    Yasuhiro MIKI, Hiromichi NIIKAWA, Keiko ABE, Satoshi SUZUKI, Shuko HATA, Jun-ichi AKAHIRA, Takashi SUZUKI, Hironobu SASANO

    13th International Congress on Hormonal Steroids and Hormones & Cancer　2008/09/27

  297. Intracrinology of Estrogen and Androgens in Human Breast Carcinoma International-presentation

    Hironobu SASANO, Yasuhiro MIKI, Shuji NAGASAKI, Takashi SUZUKI

    13th International Congress on Hormonal Steroids and Hormones & Cancer　2008/09/27

  298. Immunolocalization of COUP-TF？ in human breast carcinoma : a possible prognostic factor International-presentation

    Shuji NAGASAKI, Takashi SUZUKI, Yasuhiro MIKI, Jun-ichi AKAHIRA, Hironobu SASANO

    13th International Congress on Hormonal Steroids and Hormones & Cancer　2008/09/27

  299. INTRACRINOLOGY OF SEX-STEROIDS IN DUCTAL CARCINOMA IN SITU (DCIS) OF THE BREAST: COMPARISON TO INVASIVE DUCTAL CARCINOMA (IDC) AND NON-NEOPLASTIC BREAST International-presentation

    Hironobu SASANO, Yasuhiro MIKI, Rie SHIBUYA, Takashi SUZUKI

    18th INTERNATIONAL SYMPOSIUM of the JOURNAL OF STEROID BIOCHEMISTRY & MOLECULAR BIOLOGY　2008/09/18

  300. ヒト骨肉腫における性ステロイドホルモンの作用

    土肥 修, 羽鳥正仁, 鈴木 貴, 小野克彦, 保坂正美, 赤平純一, 三木康宏, 長崎修治, 井樋栄二, 笹野公伸

    第41回 日本整形外科学会 骨・軟部腫瘍学術集会　2008/07/17

  301. ヒト骨組織におけるSteroid and Xenobiotic Receptorの発現とその作用

    三木康宏, 長崎修治, 端 秀子, 赤平純一, 鈴木 貴, 笹野公伸

    第35回日本トキシコロジー学会学術年会　2008/06/26

  302. ヒト肝組織における薬物代謝酵素発現パターンの個人差の解析

    端 秀子, 三木康宏, 藤島史喜, 佐藤龍一郎, 海野倫明, 笹野公伸

    第35回日本トキシコロジー学会学術年会　2008/06/26

  303. Comparative effects of raloxifene, tamoxifen and estradiol on human osteoblasts in vitro International-presentation

    Yasuhiro MIKI, Shuji NAGASAKI, Shuko HATA, Jun-ichi AKAHIRA, Takashi SUZUKI, Hironobu SASANO

    The Endocrine Society’s 90th Annual Meeting　2008/06/15

  304. The expression of steroid sulfatase and estrogen sulfotransferase in human epithelial ovarian cancer International-presentation

    Jun-ichi AKAHIRA, Takashi SUZUKI, Fumihiko SUZUKI, Ikumi MIURA, Yasuhiro MIKI, Kiyoshi ITO, Nobuo YAEGASHI, Hironobu SASANO

    The Endocrine Society’s 90th Annual Meeting　2008/06/15

  305. ヒト肺癌におけるaromataseの発現

    三木康宏, 新井川弘道, 鈴木 貴, 青木貴央, 端 秀子, 長崎修治, 阿部佳子, 赤平純一, 鈴木 聡, 近藤 丘, 笹野公伸

    第81回日本内分泌学会学術総会　2008/05/16

  306. ヒト上皮性卵巣癌におけるSteroid sulfatase (STS) およびEstrogen sulfotransferase (EST) の発現について

    赤平純一, 鈴木 貴, 鈴木史彦, 三木康宏, 伊藤 潔, 八重樫伸生, 笹野公伸

    第81回日本内分泌学会学術総会　2008/05/16

  307. 乳癌における17beta-hydroxysteroid dehydrogenase type12の機能に関する検討

    長崎修治, 三木康宏, 赤平純一, 鈴木 貴, 笹野公伸

    第81回日本内分泌学会学術総会　2008/05/16

  308. New Developments in intracrinology of human breast cancer -estrogen sulfatase and sulfotransferase- International-presentation

    Hironobu SASANO, Shuji NAGASAKI, Yasuhiro MIKI, Takashi SUZUKI

    9th Advanced Course on: Steroid Enzymes and Cancer　2008/05/03

  309. 17β-hydroxysteroid dehydrogenase (17β-HSD) in human breast and endometrial carcinoma International-presentation

    Hironobu SASANO, Shuji NAGASAKI, Yasuhiro MIKI, Takashi SUZUKI

    9th Advanced Course on: Steroid Enzymes and Cancer　2008/05/03

  310. 肺癌細胞におけるエストロゲン合成酵素aromataseを介した細胞増殖作用

    三木康宏, 端 秀子, 阿部佳子, 赤平純一, 鈴木 貴, 笹野公伸

    第4 回特定非営利活動法人東北内分泌研究会総会　2008/04/26

  311. 非小細胞癌におけるestrogen receptorβとアロマターゼ発現の有意な相関

    神 幸希, 阿部佳子, 工藤信隆, 森 美紀, 三木康宏, 笹野公伸

    第4 回特定非営利活動法人東北内分泌研究会総会　2008/04/26

  312. 非浸潤性乳管癌における性ホルモン濃度と性ホルモン合成酵素の発現

    鈴木 貴, 渋谷里絵, 三木康宏, 吉田きま子, 赤平純一, 石田孝宣, 平川 久, 笹野公伸

    第4 回特定非営利活動法人東北内分泌研究会総会　2008/04/26

  313. 乳癌における17beta-hydroxysteroid dehydrogenase type12の機能に関する検討

    長崎修治, 鈴木 貴, 三木康宏, 赤平純一, 笹野公伸

    第4 回特定非営利活動法人東北内分泌研究会総会　2008/04/26

  314. 肺癌におけるエストロゲン合成酵素aromataseの発現

    三木康宏, 新井川弘道, 阿部佳子, 赤平純一, 鈴木 貴, 鈴木 聡, 笹野公伸

    第16回バイオフィジオロジー研究会　2008/02/29

  315. 骨芽細胞に対するエストロゲン受容体作動薬の直接作用の比較

    三木康宏, 端 秀子, 長崎修治, 赤平純一, 鈴木 貴, 笹野公伸

    第29回東北骨代謝研究会　2008/02/02

  316. New Developments in intracrinology of human breast cancer -estrogen sulfatase and sulfotransferase-．

    Sasano H, Nagasaki S, Miki Y

    9th Advanced Course on: Steroid Enzymes and Cancer (Symposium)　2008

  317. 骨芽細胞に対するSteroid and Xenobiotic Receptorを介したBisphenol Aの作用

    三木康宏, 長崎修治, 小野克彦, 赤平純一, 鈴木 貴, 笹野公伸

    第15回日本ステロイドホルモン学会学術集会　2007/11/24

  318. ヒト組織中ステロイドホルモン測定の臨床的病理的有用性：ホルモン依存性癌を例に

    鈴木 貴, 三木康宏, 赤平純一, 笹野公伸

    第15回日本ステロイドホルモン学会学術集会　2007/11/24

  319. 子宮内膜癌細胞におけるエストロゲン合成・代謝酵素の発現 縲怺熏ラ胞のエストロゲン合成に対する間質細胞の影響縲鰀

    宇都宮裕貴, 伊藤 潔, 小林里香, 高橋尚美, 三木康宏, 鈴木 貴, 本間誠次郎, 林 慎一, 笹野公伸, 八重樫伸生

    第15回日本ステロイドホルモン学会学術集会　2007/11/24

  320. 乳癌における17beta-hydroxysteroid dehydrogenase type12の機能に関する検討

    長崎修治, 三木康宏, 赤平純一, 鈴木 貴, 笹野公伸

    第15回日本ステロイドホルモン学会学術集会　2007/11/24

  321. 大腸癌におけるSteroid sulfataseとEstrogen sulfotransferaseの発現意義

    佐藤龍一郎, 鈴木 貴, 三木康宏, 三浦 康, 片寄 友, 海野倫明, 笹野公伸

    第15回日本ステロイドホルモン学会学術集会　2007/11/24

  322. ヒト骨肉腫組織における性ステロイドホルモンの作用

    土肥 修, 羽鳥正仁, 鈴木 貴, 小野克彦, 保坂正美, 赤平純一, 三木康宏, 長崎修治, 井樋栄二, 笹野公伸

    第15回日本ステロイドホルモン学会学術集会　2007/11/24

  323. 非小細胞肺癌における組織内Estradiol濃度とEstrogen receptor発現の関係

    新井川弘道, 鈴木 貴, 三木康宏, 鈴木 聡, 近藤 丘, 笹野公伸

    第15回日本ステロイドホルモン学会学術集会　2007/11/24

  324. ヒト肺癌組織におけるaromatase の発現

    三木康宏, 新井川弘道, 鈴木 貴, 青木貴央, 端 秀子, 赤平純一, 鈴木 聡, 近藤 丘, 笹野公伸

    第3 回特定非営利活動法人東北内分泌研究会総会　2007/10/13

  325. 大腸癌におけるSteroid sulfatase とEstrogen sulfotransferase の発現と予後の検討

    佐藤龍一郎, 鈴木 貴, 三木康宏, 三浦 康, 片寄 友, 海野倫明, 笹野公伸

    第3 回特定非営利活動法人東北内分泌研究会総会　2007/10/13

  326. The possible role of 17beta-hydroxysteroid dehydrogenase type12 in human breast cancer

    長崎修治, 三木康宏, 赤平純一, 鈴木 貴, 笹野公伸

    第66回日本癌学会学術総会　2007/10/03

  327. Sex Steroids and human osteosarcoma - Hormone dependent cell proliferation and its clinicopathological significance -

    土肥 修, 赤平純一, 鈴木 貴, 三木康宏, 笹野公伸

    第66回日本癌学会学術総会　2007/10/03

  328. The expression of estrogen receptor-related receptors in human epithelial ov arian cancer

    赤平純一, 鈴木 貴, 鈴木史彦, 三浦伊久美, 三木康宏, 長崎修治, 伊藤 潔, 八重樫伸生, 笹野公伸

    第66回日本癌学会学術総会　2007/10/03

  329. Analysis of CpG island methylator phenotype in human epithelial ovarian cancer

    鈴木史彦, 赤平純一, 三浦伊久美, 鈴木 貴, 三木康宏, 伊藤 潔, 八重樫伸生, 笹野公伸

    第66回日本癌学会学術総会　2007/10/03

  330. ヒト前立腺癌におけるEstrogen Receptor β cxの機能解析

    長崎修治, 中村保宏, 三木康宏, 赤平純一, 鈴木 貴, 林 慎一, 笹野公伸

    第8回 ホルモンと癌研究会　2007/07/27

  331. ヒト骨芽細胞における内分泌撹乱物質の影響

    三木康宏, 長崎修治, 赤平純一, 五十嵐勝秀, 菅野 純, 鈴木 貴, 笹野公伸

    第34回日本トキシコロジー学会学術年会　2007/06/27

  332. ヒト骨芽細胞における内分泌撹乱物質Bisphenol Aの細胞増殖作用

    三木康宏, 長崎修治, 赤平純一, 鈴木 貴, 笹野公伸

    第80回 日本内分泌学会学術総会　2007/06/14

  333. ヒト乳癌におけるEGR3（early growth response 3）の発現意義

    鈴木 貴, 三木康宏, 森谷卓也, 赤平純一, 石田孝宣, 山口ゆり, 林 慎一, 笹野公伸

    第80回 日本内分泌学会学術総会　2007/06/14

  334. 前立腺癌におけるEstrogen Receptor β 及び βcxの機能解析

    長崎修治, 中村保宏, 三木康宏, 赤平純一, 鈴木 貴, 林 慎一, 笹野公伸

    第80回 日本内分泌学会学術総会　2007/06/14

  335. ヒト上皮性卵巣癌におけるEstrogen receptor-related receptor (ERR)βの発現と臨床病理学的因子との関連について

    赤平純一, 鈴木 貴, 鈴木史彦, 三浦伊久美, 三木康宏, 長崎修治, 鴨川由紀子, 伊藤 潔, 森谷卓也, 林 慎一, 八重樫伸生, 笹野公伸

    第80回 日本内分泌学会学術総会　2007/06/14

  336. Early growth responsive gene 3 (EGR3) in human breast carcinoma: aregulator of estrogen-meditated invasion and a potent prognostic factor International-presentation

    Takashi SUZUKI, Yasuhiro MIKI, Takuya MORIYA, Jun-ichi AKAHIRA, Takanori ISHIDA, Yuri YAMAGUCHI, Shin-ichi HAYASHI, Hironobu SASANO

    The Endocrine Society’s 89th Annual Meeting　2007/06/02

  337. The expression of estrogen-related receptors in human epithelial ovarian cancer: The clinical significance of ERRα in clear cell adenocarcinoma International-presentation

    Jun-ichi AKAHIRA, Takashi SUZUKI, Fumihiko SUZUKI, Ikumi MIURA, Noriko, KAMOGAWA, Yasuhiro MIKI, Kiyoshi ITO, Nobuo YAEGASHI, Hironobu SASANO

    The Endocrine Society’s 89th Annual Meeting　2007/06/02

  338. 骨芽細胞に対するSERMsの直接作用の比較

    三木康宏, 長崎修治, 赤平純一, 鈴木 貴, 笹野公伸

    第3回 SERM学術研究会学術集会　2007/05/12

  339. 86. ヒト骨芽細胞に対するビスフェノール Aの影響

    三木康宏, 長崎修治, 赤平純一, 鈴木 貴, 笹野公伸

    第14回 日本内分泌学会東北地方会（第59回東北内分泌研究会）　2007/04/28

  340. 87. ヒト骨肉腫組織における性ステロイドホルモンの作用

    土肥 修, 鈴木 貴, 小野克彦, 赤平純一, 三木康宏, 保坂正美, 羽鳥正仁, 笹野公伸

    第14回 日本内分泌学会東北地方会（第59回東北内分泌研究会）　2007/04/28

  341. 乳癌組織におけるEGR3（early growth response 3）の発現意義

    鈴木 貴, 三木康宏, 森谷卓也, 赤平純一, 石田孝宣, 山口ゆり, 林 慎一, 笹野公伸

    第14回 日本内分泌学会東北地方会（第59回東北内分泌研究会）　2007/04/28

  342. 非浸潤性乳管癌における性ステロイドホルモン濃度および性ステロイド合成酵素の発現について

    渋谷里絵, 鈴木 貴, 三木康宏, 森谷卓也, 小野克彦, 赤平純一, 石田孝宣, 平川 久, 笹野公伸

    第14回 日本内分泌学会東北地方会（第59回東北内分泌研究会）　2007/04/28

  343. 前立腺癌におけるEstrogen Receptor beta cxの機能解析

    長崎修治, 中村保宏, 三木康宏, 赤平純一, 鈴木 貴, 林 慎一, 笹野公伸

    第14回 日本内分泌学会東北地方会（第59回東北内分泌研究会）　2007/04/28

  344. RIZ1 expression in the patients with epithelial ovarian carcinoma: Its correlation with aberrant DNA methylation International-presentation

    Jun-ichi AKAHIRA, Hirofumi SUZUKI, Ikumi MIURA, Takashi SUZUKI, Takuya MORIYA, Yasuhiro MIKI, Kiyoshi ITO, Shinichi HAYASHI, Nobuo YAEGASHI, Hironobu SASANO

    American Association for Cancer Research 98th Annual Meeting　2007/04/14

  345. ヒト骨芽細胞におけるエストロゲン及びSERMの直接作用の比較

    三木康宏, 長崎修治, 赤平純一, 鈴木 貴, 笹野公伸

    第14回 日本ステロイドホルモン学会　2006/12/09

  346. 乳癌組織におけるアンドロゲン濃度

    鈴木 貴, 三木康宏, 森谷卓也, 赤平純一, 石田孝宣, 平川 久, 山口ゆり, 林 慎一, 笹野公伸

    第14回 日本ステロイドホルモン学会　2006/12/09

  347. 前立腺癌細胞におけるEstrogen Receptor beta 及び beta cxの機能解析

    長崎修治, 中村保宏, 三木康宏, 赤平純一, 鈴木 貴, 林 慎一, 久田 茂, 堀内 敏, 笹野公伸

    第14回 日本ステロイドホルモン学会　2006/12/09

  348. ヒト上皮性卵巣癌におけるEstrogen related receptor (ERR) αの発現と臨床病理学的因子との関連について

    赤平純一, 鈴木 貴, 三木康宏, 長崎修治, 伊藤 潔, 森谷卓也, 林 慎一, 八重樫伸生, 笹野公伸

    第14回 日本ステロイドホルモン学会　2006/12/09

  349. ヒト骨芽細胞における内分泌撹乱化学物質の影響

    三木康宏, 鈴木 貴, 笹野公伸

    第13回 日本内分泌学会東北地方会（第58回東北内分泌研究会）　2006/10/14

  350. ヒト上皮性卵巣癌におけるEstrogen related receptor (ERR)αの発現と臨床病理学的因子との関連について

    赤平純一, 鈴木 貴, 三木康宏, 長崎修治, 伊藤 潔, 森谷卓也, 林 慎一, 八重樫伸生, 笹野公伸

    第13回 日本内分泌学会東北地方会（第58回東北内分泌研究会）　2006/10/14

  351. 前立腺癌細胞におけるEstrogen Receptor beta 及び beta cxの機能解析

    長崎修治, 中村保宏, 三木康宏, 赤平純一, 鈴木 貴, 林 慎一, 笹野公伸

    第13回 日本内分泌学会東北地方会（第58回東北内分泌研究会）　2006/10/14

  352. ヒト乳癌組織における性ステロイド合成

    鈴木 貴, 森谷卓也, 三木康宏, 平川 久, 林 慎一, 笹野公伸

    第38回日本臨床分子形態学会総会ならびに学術講演会　2006/09/29

  353. ヒト乳癌アロマターゼの腫瘍内局在と発現機序の検討

    三木康宏, 鈴木 貴, 山口ゆり, 森谷卓也, 林 慎一, 大内憲明, 笹野公伸

    第65回日本癌学会学術総会　2006/09/28

  354. 卵巣癌におけるRIZ1の発現と遺伝子プロモーター領域のDNAメチル化について

    赤平純一, 鈴木 貴, 伊藤 潔, 森谷卓也, 八重樫伸生, 三木康宏, 長崎修治, 笹野公伸

    第65回日本癌学会学術総会　2006/09/27

  355. Aromatase localization in human breast carcinoma: a possible interaction between intratumoral stromal and parenchymal cells International-presentation

    Yasuhiro MIKI, Takashi SUZUKI, Yuri YAMAGUCHI, Seiziro HONMA, Takuya MORIYA, Dean EVANS, Shin-ich HAYASHI, Noriaki OHUCHI, Hironobu SASANO

    12th International Congress on Hormonal Steroids and Hormones & Cancer　2006/09/13

  356. Expression of estrogen related genes (EBAG9 and efp) and its clinicopathological significance in patients with epithelial ovarian cancer International-presentation

    Jun-ichi AKAHIRA, Michiko SAKUMA, Takashi SUZUKI, Yasuhiro MIKI, Takuya MORIYA, Nobuo YAEGASHI, Hironobu SASANO

    12th International Congress on Hormonal Steroids and Hormones & Cancer　2006/09/13

  357. ヒト乳癌細胞におけるアロマターゼの発現：癌細胞/間質細胞の相互作用

    三木康宏, 山口ゆり, 本間誠次郎, 鈴木 貴, 林 慎一, 笹野公伸

    第7回 ホルモンと癌研究会　2006/06/30

  358. 乳癌のIntracrinology

    笹野公伸, 三木康宏, 鈴木 貴

    第7回 ホルモンと癌研究会　2006/06/30

  359. Peroxisome proliferator-activated receptor γ (PPARγ) in human breast carcinoma: a possible modulator of estrogenic actions International-presentation

    Takashi SUZUKI, Shin-ichi HAYASHI, Yasuhiro MIKI, Yasuhiro NAKAMURA, Takuya MORIYA, Akira SUGAWARA, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO

    The Endocrine Society’s 88th Annual Meeting　2006/06/24

  360. ヒト乳癌組織におけるアロマターゼの局在：乳癌細胞および周囲間質細胞の相互作用

    三木康宏, 田澤智香, 鈴木 貴, 山口ゆり, 本間誠次郎, 北田邦雄, 林 慎一, 笹野公伸

    第3回 東北大学バイオサイエンスシンポジウム　2006/05/29

  361. 内分泌撹乱物質：Genistein, Bisphenol A, Diethylstilbestrolのヒト骨芽細胞に対する影響

    三木康宏, 鈴木 貴, 五十嵐勝秀, 相崎健一, 小野克彦, 菅野 純, 笹野公伸

    第79回 日本内分泌学会学術総会　2006/05/19

  362. 乳癌組織における核内受容体peroxisome proliferator-activated receptor (PPAR) γ の発現意義

    鈴木 貴, 三木康宏, 森谷卓也, 石田孝宣, 大内憲明, 林 慎一, 笹野公伸

    第79回 日本内分泌学会学術総会　2006/05/19

  363. 乳癌組織における核内受容体LRH-1の発現意義

    鈴木 貴, 三木康宏, 渋谷理絵, 中村保宏, 森谷卓也, 笹野公伸

    第95回 日本病理学会総会　2006/04/30

  364. ヒト骨芽細胞におけるselective estrogen receptor modulatorsの影響

    三木康宏, 鈴木 貴, 笹野公伸

    第12回 日本内分泌学会東北地方会（第57回東北内分泌研究会）　2006/04/08

  365. 乳癌組織におけるLiver Receptor homologue-1 (LRH-1) の発現

    鈴木 貴, 三木康宏, 中村保宏, 森谷卓也, 笹野公伸

    第12回 日本内分泌学会東北地方会（第57回東北内分泌研究会）　2006/04/08

  366. ヒトカルチノイド腫瘍におけるソマトスタチン受容体（SSTR）の発現とソマトスタチンアナログの効果

    笹野公伸, 小野克彦, 鈴木 貴, 三木康宏, 谷山裕亮, 中村保宏

    第1回 Neuroendocrine Tumor Workshop Japan　2006/01/21

  367. Effects of aromatase inhibitors on human osteoblast cells: a possible androgenic bone protective effects induced by exemestane. International-presentation

    Yasuhiro MIKI, Takashi SUZUKI, Katsuhide IGARASHI, Jun KANNO, Hironobu SASANO

    28th Anneal San Antonio Breast Cancer Symposium　2005/12/08

  368. ヒト骨芽細胞におけるアロマターゼ阻害剤の影響

    三木康宏, 鈴木 貴, 五十嵐勝秀, 菅野 純, 笹野公伸

    第13回 日本ステロイドホルモン学会　2005/11/12

  369. ヒト乳癌におけるperoxisome proliferator-activated receptor (PPAR)γの発現意義：エストロゲン作用調節因子の可能性

    鈴木 貴, 三木康宏, 森谷卓也, 石田孝宣, 菅原 明, 林 慎一, 大内憲明, 笹野公伸

    第11回 日本内分泌学会東北地方会（第56回東北内分泌研究会）・第17回青森県内分泌研究会　2005/11/12

  370. カルチノイド細胞におけるソマトスタチンの作用

    小野 克彦, 鈴木 貴, 三木康宏, 谷山裕亮, 中村保宏, 笹野公伸

    第10回 日本内分泌学会東北地方会（第55回東北内分泌研究会）　2005/10/01

  371. ヒト乳癌細胞及び周囲間質細胞におけるアロマターゼの発現

    三木康宏, 田澤智香, 小野克彦, 鈴木 貴, 笹野公伸

    第6回 ホルモンと癌研究会　2005/07/15

  372. 腫瘍内ホルモン代謝酵素−EndocrinologyからIntracrinologyへの概念の転換−

    笹野公伸, 三木康宏, 中村保宏, 鈴木 貴

    第6回 ホルモンと癌研究会　2005/07/15

  373. カルチノイド組織におけるソマトスタチン受容体 (SSTR) の発現

    小野 克彦, 鈴木 貴, 三木康宏, 谷山裕亮, 中村保宏, 笹野公伸

    第46回 日本組織細胞化学会総会・学術総会　2005/07/15

  374. 内分泌撹乱物質のヒト骨芽細胞に対する影響：マイクロアレイによる遺伝子発現解析

    三木康宏, 鈴木 貴, 五十嵐勝秀, 相崎健一, 菅野 純, 中村保宏, 井上 達, 笹野公伸

    第78回 日本内分泌学会学術総会　2005/07/01

  375. 乳癌組織におけるestrogen-related receptor α (ERRα)の発現

    鈴木 貴, 三木康宏, 森谷卓也, 石田孝宣, 井上 聡, 大内憲明, 笹野公伸

    第78回 日本内分泌学会学術総会　2005/07/01

  376. カルチノイド細胞におけるソマトスタチンの作用

    小野 克彦, 鈴木 貴, 三木康宏, 谷山裕亮, 中村保宏, 笹野公伸

    第78回 日本内分泌学会学術総会　2005/07/01

  377. Estrogen-related receptor α (ERRα) in human breast carcinoma as a potent prognostic factor International-presentation

    Takashi SUZUKI, Yasuhiro MIKI, Takuya MORIYA, Norihiro SHIMADA, Takanori ISHIDA, Hisashi HIRAKAWA, Noriaki OHUCHI, Hironobu SASANO

    The Endocrine Society’s 87th Annual Meeting　2005/06/04

  378. ヒト成人組織における17β-hydoroxysteroid dehydrogenase type 12 の発現

    三木康宏, 櫻井信行, 鈴木寿恵, 佐々木亜由美, 鈴木 貴, 笹野公伸

    第10回 日本内分泌学会東北地方会（第55回東北内分泌研究会）　2005/04/23

  379. ヒト乳癌組織におけるSteroid and Xenobiotic Receptor (SXR), CYP3A4, MDR1の発現

    三木康宏, 鈴木 貴, 田澤智香, 松井 恵, 石田孝宣, 平川 久, Bruce Blumberg, 笹野公伸

    第12回 日本ステロイドホルモン学会　2004/11/20

  380. ヒト乳癌組織におけるSteroid and Xenobiotic Receptor及び応答遺伝子の解析

    三木康宏, 鈴木 貴, 田澤智香, 小野克彦, 笹野公伸

    第9回 日本内分泌学会東北地方会（第54回東北内分泌研究会）　2004/10/02

  381. ヒト副腎組織におけるNGFI-Bファミリーの発現

    吉川 陽介, 鈴木 貴, 三木康宏, 村上 治, 笹野公伸

    第77回 日本内分泌学会学術総会　2004/06/24

  382. 48. エストロゲン作用調節に関与する核内受容体の乳癌組織での発現

    鈴木 貴, 三木康宏, 笹野公伸

    第77回 日本内分泌学会学術総会　2004/06/24

  383. Interactions between aromatase, prostaglandin E2 and LRH-1 in breast cancer International-presentation

    Colin D. CLYNE, Takashi SUZUKI, Agnes KOVACIC, Jiong ZHOU, Ryoko SAITO, Yasuhiro MIKI, Takanori ISHIDA, Takuya MORIYA, Hironobu SASANO, Evan R. SIMPSON

    The Endocrine Society’s 86th Annual Meeting　2004/06/16

  384. ヒト乳癌組織におけるperoxisome proliferator-activated receptor (PPAR)gの発現

    鈴木 貴, 中林正雄, 三木康宏, 森谷卓也, 石田孝宣, 大内憲明, 笹野公伸

    第12回 日本乳癌学会総会　2004/06/11

  385. ヒト副腎皮質腫瘍におけるNurr1及びNGFI-Bの発現

    鈴木 貴, 三木康宏, 森谷卓也, 笹野公伸

    第93回 日本病理学会総会　2004/06/09

  386. ヒト乳癌組織におけるSteroid and Xenobiotic Receptor (SXR) 及び代謝酵素の解析

    三木康宏, 鈴木 貴, 田澤智香, 石田孝宣, Bruce Blumberg, 笹野公伸

    第1回 東北大学バイオサイエンスシンポジウム　2004/05/14

  387. ヒト乳癌組織におけるsteroid and xenobiotic receptor (SXR) の発現

    三木康宏, 鈴木 貴, 金子智香, 松井 恵, 笹野公伸

    第8回 日本内分泌学会東北地方会（第53回東北内分泌研究会）　2004/04/24

  388. 乳癌細胞株を用いたSTS発現制御解析の基礎検討

    福田 剛, 鈴木 貴, 三木康宏, 田澤智香, 小野克彦, 笹野公伸

    第8回 日本内分泌学会東北地方会（第53回東北内分泌研究会）　2004/04/24

  389. ヒト乳癌組織におけるperoxisome proliferator-activated receptor (PPAR)gの発現

    鈴木 貴, 中林正雄, 三木康宏, 森谷卓也, 石田孝宣, 大内憲明, 笹野公伸

    第8回 日本内分泌学会東北地方会（第53回東北内分泌研究会）　2004/04/24

  390. 乳癌におけるLiver Receptor Homologue-1 (LRH-1) の発現

    鈴木 貴, 斉藤涼子, 三木康宏, 笹野公伸

    第11回 日本ステロイドホルモン学会　2003/11/22

  391. ヒト乳癌組織におけるLiver Receptor Homologue-1 (LRH-1) の発現

    斉藤涼子, 鈴木 貴, 三木康宏, 笹野公伸

    第62回 日本癌学会総会　2003/09/25

  392. Estrogen Sulfotransferase (EST) and steroid Sulfatase (STS) in Human Breast Carcinoma International-presentation

    Takashi SUZUKI, Taisuke NAKATA, Yasuhiro MIKI, Takuya MORIYA, Hironobu SASANO

    The Endocrine Society’s 85th Annual Meeting　2003/06/19

  393. ヒト成人及び胎児組織におけるSXR、CYP3A及びMDR1の発現

    三木康宏, 鈴木 貴, 田澤智香, Bruce Blumberg, 笹野公伸

    第76回 日本内分泌学会学術総会　2003/05/09

  394. Laser capture microscopy

    鈴木 貴, 田澤智香, 三木康宏, 森谷卓也, 笹野公伸

    第76回 日本内分泌学会学術総会　2003/05/09

  395. ヒト大動脈におけるPRの発現およびプロゲステロンの局所産生

    中村保宏, 鈴木 貴, 井上 宰, 三木康宏, 田澤智香, 小野克彦, 松井 恵, 森谷卓也, 笹野公伸

    第76回 日本内分泌学会学術総会　2003/05/09

  396. ヒト乳癌組織におけるsteroid sulfatase及びestrogen sulfotransferaseの発現意義

    鈴木 貴, 三木康宏, 森谷卓也, 石田孝宣, 中田泰介, 塩津行正, 秋永士郎, 井上謙吾, 木村道夫, 笹野公伸

    第92回 日本病理学会学術総会　2003/04/23

  397. 乳癌組織におけるLRH-1（liver receptor homologue-1）の発現

    斉藤涼子, 鈴木 貴, 三木康宏, 笹野公伸

    第6回 日本内分泌学会東北地方会（第51回東北内分泌研究会）　2003/04/12

  398. ヒト全身組織におけるNGFI-B及びNurr1の発現

    吉川陽介, 鈴木 貴, 三木康宏, 笹野公伸

    第6回 日本内分泌学会東北地方会（第51回東北内分泌研究会）　2003/04/12

  399. 正常副腎及び副腎腫瘍におけるNGFI-B及びNurr1の発現

    鈴木 貴, 吉川陽介, 三木康宏, 笹野公伸

    第6回 日本内分泌学会東北地方会（第51回東北内分泌研究会）　2003/04/12

  400. ヒト全身組織におけるDAX-1の発現

    佐藤容子, 鈴木 貴, 三木康宏, 金子智香, 笹野公伸

    第6回 日本内分泌学会東北地方会（第51回東北内分泌研究会）　2003/04/12

  401. ヒト大動脈におけるプロゲステロンレセプターの発現およびプロゲステロンの局所産生

    中村保宏, 鈴木 貴, 井上 宰, 三木康宏, 田澤智香, 小野克彦, 松井 恵, 森谷卓也, 笹野公伸, 斉藤春夫, 石橋忠司, 高橋昭喜

    第6回 日本内分泌学会東北地方会（第51回東北内分泌研究会）　2003/04/12

  402. ヒト成人及び胎児組織におけるsteroid sulfatase及びestrogen sulfotransferaseの分布

    三木康宏, 鈴木 貴, 金子智香, 日高久美子, 中田泰介, 塩津行正, 秋永士郎, 井上謙吾, 笹野公伸

    第75回 日本内分泌学会学術総会　2002/06/28

  403. Estrogen Receptor A and B Expression in the Human Athelosclerotic Aorta International-presentation

    Yasuhiro NAKAMURA, Takashi SUZUKI, Yasuhiro MIKI, Andrew David Darnel, Kumiko HIDAKA, Chika KANEKO, Hironobu SASANO

    The 84th Annual Meeting of The Endocrine Society　2002/06/19

  404. Sex steroid Receptors in the Synovial Tissue of Rheumatoid International-presentation

    Masato ISHIZUKA, Takashi SUZUKI, Yasuhiro MIKI, Chika KANEKO, Takashi SAWAI, Miwa UZUKI, Hironobu SASANO

    The 84th Annual Meeting of The Endocrine Society　2002/06/19

  405. Mineralocorticoid Receptor, type 1 and 2 11beta-Hydroxysteroid dehydrogenaze and type 3 collagen in human and SHR rat heart International-presentation

    Akinobu KONISHI, Takashi SUZUKI, Chika KANEKO, Yasuhiro MIKI, Andrew. D. Darnel, Takuya MORIYA, Koichi TABAYASHI, Hironobu SASANO

    The 28th International Aldosterone Conference　2002/06/18

  406. ヒト成人及び胎児組織におけるsteroid sulfatase及びestrogen sulfotransferaseの分布

    三木康宏, 鈴木 貴, 金子智香, 日高久美子, 中田泰介, 塩津行正, 日下英昭, 笹野公伸

    第4回 日本内分泌学会東北地方会（第49回東北内分泌研究会）　2002/04/13

  407. ヒト乳癌組織におけるsteroid sulfatase及びestrogen sulfotransferaseの発現

    鈴木 貴, 中田泰介, 三木康宏, 森谷卓也, 石田孝宣, 塩津行正, 秋永士郎, 井上謙吾, 木村道夫, 笹野公伸

    第4回 日本内分泌学会東北地方会（第49回東北内分泌研究会）　2002/04/13

  408. 乳癌組織におけるsteroid and xenobiotic receptor の発現

    三木康宏, 鈴木 貴, 金子智香, 松井 恵, 石田孝宣, 木村道夫, Bruce Blumberg, 笹野公伸

    第3回 ホルモンと癌研究会（ワークショップ）　2002

  409. Steroid Xenobiotic Recepter and Cytocrome P450 3A subfamily in Human Adult and Fetal Tissues

    Miki Y, Suzuki T, Tazawa C, Blumberg B, Sasano H.

    11th International Congress on Hormone Steroid/ 7th International Congress on Hormone and Cancer　2002

  410. Steroid and xenobiotic Receptor and estrogen synthesis/metabolite enzymes in human breast carcinoma

    Miki Y, Suzuki T, Tazawa C, Matsui M, Ishida T, Kimura M, Blumberg B, Sasano H.

    VI International Aromatase Conference　2002

  411. Steroid sulfatase and estrogen sulfotransferase in human breast carcinoma

    Suzuki T, Miki Y, Nakata T

    VI International Aromatase Conference (Symposium)　2002

  412. アルカリ性フォスファターゼ（ALP）活性の上昇と薬物代謝酵素誘導

    渡辺 大, 堀口浩資, 三木康宏, 石井俊也, 山口裕子, 笠原健一郎, 畠山和久, 斉藤 準, 長島吉和, 岡庭 梓

    第28回 日本トキシコロジー学会学術年会　2001/06/10

  413. 毒性試験における初期全身性反応 II. イヌにおけるエンドトキシン投与及び外科的侵襲時の所見

    星谷 達, 渡辺 大, 松岡哲也, 堀口浩資, 三木康宏, 水口浩康, 石井俊也, 野村典之, 長島吉和, 岡庭 梓

    第27回 日本トキシコロジー学会学術年会　2000/06/28

  414. スタウロスポリンによる血管内皮細胞のCa2+依存性及び非依存性アポトーシス

    三木康宏, 寺岡宏樹, 竹花一成, 平賀武夫

    第125回 日本獣医学会学術総会　1998/04/05

  415. Ca2+-dependent and –independent apoptosis by staurosporine in vascular endothelial cells

    寺岡宏樹, 三木康宏, 竹花一成, 平賀武夫

    第71回 日本薬理学会学術年会　1998/03/23

  416. 血管内皮細胞におけるスタウロスポリン誘発性アポトーシス

    三木康宏, 寺岡宏樹, 竹花一成, 小野寺秀一, 平賀武夫

    第11回 北海道薬物作用談話会　1997/08/02

  417. スタウロスポリンによる血管内皮細胞のアポトーシス

    三木康宏, 寺岡宏樹, 竹花一成, 平賀武夫

    第24回 日本毒科学会学術年会 1997年　1997/07/23

  418. ニコチン受容体を介するニワトリ副腎クロム親和性細胞からのカテコールアミン分泌

    松澤留美子, 丸山 豊, 三木康宏, 寺岡宏樹, 平賀武夫

    第122回 日本獣医学会学術総会　1996/08/29

  419. 培養血管内皮に対する出血性ヘビ毒の細胞障害作用

    寺岡宏樹, 横山 恵, 丸山 豊, 三木康宏, 竹花一成, 平賀武夫

    第23回 日本毒科学会学術年会　1996/07/25

  420. Cross talk between adipocytes and carcinomas in human breast cancer.

    Sasano H, Sakurai M, MikiY

    12th International Aromatase Symposium 2014

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Research Projects 32

  1. 子宮内膜癌におけるリピドミクス解析

    伊藤 潔

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04 - 2023/03

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    免疫組織化学によって、子宮内膜癌組織におけるCD36およびアシルCoAデヒドロゲナーゼ長鎖（ACADL：Acyl-CoA Dehydrogenase Long Chain）の発現を調べた。CD36は長鎖脂肪酸を輸送する膜タンパク質であり、ACADLはβ酸化の初期反応を触媒する酵素である。リンパ節転移陽性症例において、CD36陽性の割合が有意に高かった。ACADLと臨床病理学的因子との関連は認められなかった。細胞増殖に対する脂質添加の効果を検討するために、同じレベルのCD36を有する乳癌培養細胞（T-47D）および子宮内膜癌培養細胞（AN3CA）を使用した。脂質はRefeed JNS（Remembrane、イタリア）を用いた。脂質の添加では、T-47DとAN3CAの両方において、非添加群と比べて添加群で有意な細胞数の増加を認めた。さらに細胞内脂肪酸のβ酸化を可視化するFAOBlue（フナコシ、日本）による検討を行った。FAOBlueは、青色蛍光色素クマリンの誘導体を含むノナン酸（C9）であり、4回目のFAOサイクルでFAOBlueが分解された後、クマリンはプロピオン酸から放出され、放出されたクマリンは405nmで励起された強い青色の蛍光を示す。脂質添加培地では、T-47Dはβ酸化活性を示す青色蛍光を示した。一方、AN3CAでは同じ条件下で蛍光が非常に弱かった。乳癌および子宮内膜癌は、細胞内脂質の取り込みとそれに続くβ酸化の経路を有すると考えられた。組織解析から、子宮内膜癌の脂質の影響には、脂質の取り込みが重要であることが示唆された。取り込まれた脂肪酸の増殖や浸潤に対するメカニズムに関して、複数での培養細胞での検討とリピドミクス解析による脂肪酸代謝の検討を行う。

  2. The stress hormone and carcinogenic potential

    Miki Yasuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    Many studies have suggested a relationship between stress and carcinogenesis, but the mechanism remains unclear. In this study, we focused on the effects of cortisol, which secretion increases with stress. The results of this study revealed that in endometrial cancer, intratumoral cortisol concentration is related to the expression of cellular senescence markers in cancer cells. Intratumoral cortisol also affected the number of exhausted T lymphocytes in endometrial cancer microenvironment. It has been suggested that stress not only causes cellular senescence by increasing cortisol secretion, but also affects the entire microenvironment such as tumor immunity.

  3. The establishment of developed bone regeneration using time-lag release material

    Shimizu Yoshinaka

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2017/04/01 - 2021/03/31

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    In this study, the biocompatibility of new hydroxyapatite as a material having a drug-carrying structure that enables time-lag release, was examined in the viewpoint of fibrous encapsulation and inflammation. And in vivo decomposition behavior and osteoconductive of the material was clarified by histopathological and histomorphometric examinations. This hydroxyapatite was immersed with a bone marrow stem cell-derived extracted protein solution and applied bone defect to confirm the bone formation effect. This hydroxyapatite composed of ultralong hydroxyapatite nanowire has excellent drug-carrying property, and promotes the new bone formation in early time. The hydroxyapatite containing bone marrow stem cell-derived extracted protein solution promote new bone formation more promptly. The multi-layered structure of this material would enable tissue regeneration by acting on multiple tissues due to the drug action at different times.

  4. 研究者にとってのニュー・ノーマル：COVID-19は研究者の研究環境とモチベーションを変えたのか？

    三木康宏

    Offer Organization: 新型コロナウイルス対応研究支援プロジェクト（東北大学災害科学国際研究所）

    2020/04 - 2021/03

  5. Effects of stress hormone on hormone metabolism and cancer growth in endometrial cancer

    Miki Yasuhiro, ITO Kiyoshi, TAKAGI Kiyoshi, YOSHIDA Rena, FUE Misaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    Intratumoral cortisol concentration was considered to be regulated by 11β-hydroxysteroid dehydrogenase (HSD) 1 and 11β-HSD2 in endometrial cancer. In endometrial cancer, cortisol induced enzymatic activity of estrogen synthase (aromatase) through glucocorticoid receptor (GR) located in the stromal cells but nor carcinoma cells. This finding suggests that cortisol contributed estrogen-dependency of endometrial cancer cells. It is also suggested that cortisol directly relates to cell growth through the induction of annexin A1 expression, which is known as a GR target gene, in stromal cells of endometrial cancer. Dhydroepiandrosterone (DHEA), which is also known as a stress-related hormone, was synthesized by steroid sulfatase in endometrial cancer tissue.

  6. In situ visualization of HER2 signal in archival breast cancer specimens.

    Sasano Hironobu, MIKI Yasuhiro, ONO Katsuhiko, ONODERA Yoshiaki, IWABUCHI Erina

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2017/03/31

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    It has become important to identify the surrogate markers regarding HER2 inhibitor sensitivity for HER2-positive breast cancer patients. In this study, we focused on protein-protein interaction (PPI) between HER2 and carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) in breast cancer cells. In breast cancer RT-474 cells, HER2/CEACAM6 PPI was detected by using co-immunoprecipitation assay and visualized by using both proximity ligation assay (PLA) and high resolution microscopy. We then visualized HER2/CEACAM6 PPI by using PLA in human breast carcinoma tissues. Knockdown of CEACAM6 gene significantly decreased the anti-cell proliferative effect of HER2 inhibitor in RT-474. These findings suggest that CEACAM6 has an effect by PPI with HER2 on HER2 sensitivity in breast cancer cells.

  7. Progesteron Receptor Expression in Pancreatic Neuroendocrine Tumor

    KASAJIMA ATSUKO, SASANO Hironobu, NAKAMURA Yasuhiro, MIKI Yasuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    Progesteron receptor: PR is frequently expressed in pancreatic neuroendocrine tumor: PNET and is related to a longer prognosis of the patients. However, its mechanism is not clarified. Expression of PR subtype A (PRA) was negatively associated with multiple cell cycle regulators and with proliferative activities in PNET tissues. In addition, expression induced decrease expression of multiple cell cycle related gene (CCNA2, CCNB1, CDK1, CDK2) and inhibited cell cycle in a PNET cell line (QGP1). These results suggested that PRA is closely associated with inhibition of cell cycle and can be a target molecule in treatment for patients with PNET.

  8. Bone regeneration using condensing platelet fibrin gel and adipose-derived stem cells conditioned medium

    ITO HIDETOSHI, FUKUOKA HIROTAROU

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    The objective of this research is to investigate the effect to bone regeneration of condensing platelet fibrin gel derived from autologous blood (Concentrated Growth Factors: CGF) and adipose-derived stem cells conditioned medium(ADSCs-CM). In culture examination, the behavior of angiogenesis of CGF was confirmed. The CGF implanted subcutaneously in rabbit back was disappeared before 4 weeks after implantation. In process of disappearance, many macrophages and newly formed vessels was seen around the CGF. The compressed and membranal CGF showed excellent mechanical properties characterized by high ductility with wide clinical application. The ADSC-MC contained PDGF, VEGF, HGF,and TGF-b, which are known to be necessary for tissue regeneration. This medium contained a high level of VEGF, HGF and TGF-b.

  9. Does post-menopausal endometrioid endometrial carcinoma occur in de novo and form endometrial hyperplasia in the neighborhood?

    Ito Kiyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    We indicated that estrogen-dependent carcinoma progression is suppressed by dihydrotestosterone (DHT)-androgen receptor pathway in post-menopausal endometrioid endometrial carcinoma. Among the enzymes required for local biosynthesis of androgen, 5α-reductase type1 is the principal enzyme in the formation of DHT in endometrial carcinoma. The expression of this enzyme may play the important role for suppressing the carcinoma progression. In addition, We found that 17β-hydroxysteroid dehydrogenase type2, which catalyzes E2 to E1, is induced by DHT in post-menopausal endometrioid endometrial carcinoma.

  10. Detection of estrogen receptor dimerization patter in breast carcinoma tissues.

    SASANO HIRONOBU, MIKI Yasuhiro, IWABUCHI Erina

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    Examination of estrogen receptors (ER) is important to determine appropriate treatment options for breast cancer. ERα and ERβ dimers bind DNA with comparable affinities as either homo- or heterodimers to the same estrogen response elements. ER dimers have been identified by molecular technique but no in situ methodologies have been reported. Proximity Ligation Assay (PLA) could demonstrate the molecular proximity of two proteins in situ in tissue specimens. In this study, we established detection method of ER dimerization using PLA between specific antibodies for ERα and ERβ in breast cancer cell lines and breast carcinoma tissues.

  11. Histological comparison of fibroblastic stromal cells in cancer microenvironment

    MIKI Yasuhiro, HATA Shuko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    It is well known that intratumoral stromal cells exert important role on cancer proliferation and invasion in several types of cancer. However, the histological differences in the cell growth and angiogenesis of cancer remain unclear. In this study, we established fibroblast-like stromal cell (FSC) derived from breast and lung carcinoma tissues, respectively. There were no differences in the cancer cell growth between the FSC derived from squamous cell carcinoma and adenocarcinoma of the lung, but considerable differences in tubular formation of endothelial cells were observed between the two histologic types.

  12. Roles of cell degradation system regulation in oncogenesis of odontogenic epithleium within the intraosseous microenvironmnet

    KUMAMOTO HIROYUKI, MIKI Yasuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    Regulator molecules associated with cell proliferation and degradation as well as hypocic condition under the intraosseous microenvironment were examined in oncogenesis, cell differantiation, and progression. Growth factor receptors, EGFR and HER2-4, were expresssed in odontogenic epithelial cells, and reactivity for EGFR and HER4 was prominent. Gene alterations of these molecules were obscure. Autophagy-related molecules, ATG7, LC3, and p62, were recognized chiefly in odontogenic epithelial cells neighboring the basement membrane. Granula cell ameloblastoma showed high reactivity for these molecules. Hypoxia-related molecules, HIF-1 and CA IX, were found greater in ameloblastoma than in tooth germ, and solid type ameloblastoma showed high reactivity as compared with unicystic type ameloblastoma. Measurement of CD34-positive microvessels is in progress.

  13. ストレスホルモンと子宮内膜癌：治療標的因子としてのコルチゾールシグナルの解明

    三木康宏

    Offer Organization: 公益財団法人宮城県対がん協会 黒川利雄がん研究基金

    2013/04 - 2014/03

  14. エストロゲン依存性腫瘍における抗ホルモン療法耐性機序に関する研究

    三木康宏

    Offer Organization: 公益財団法人山口内分泌疾患研究振興財団 研究助成金

    2013/04 - 2014/03

  15. 公益財団法人ライフサイエンス振興財団 国際交流援助（研究集会）

    三木康宏

    Offer Organization: 公益財団法人ライフサイエンス振興財団

    2012/04 - 2013/03

  16. 震災時ストレスとその後の生活環境変化が婦人科疾患に及ぼす影響の解析

    三木康宏

    Offer Organization: 東北大学災害科学国際研究所特定プロジェクト研究（拠点研究）

    2012/04 - 2013/03

  17. 喫煙によるエストロゲン環境の撹乱に関する研究

    三木康宏

    Offer Organization: 公益財団法人ひと・健康・未来研究財団 研究助成

    2012/04 - 2013/03

  18. トミーデジタルバイオロジー株式会社 若手研究者応援プログラム：Travel Award 2011

    三木康宏

    Offer Organization: トミーデジタルバイオロジー株式会社

    2011/04 - 2012/03

  19. 財団法人金原一郎記念医学医療振興財団 第26回研究交流助成金

    三木康宏

    Offer Organization: 財団法人金原一郎記念医学医療振興財団

    2011/04 - 2012/03

  20. 口腔癌におけるSXRを介した薬物療法耐性機序に関する研究

    三木康宏

    Offer Organization: 財団法人工藤学術財団 学術研究補助

    2011/04 - 2012/03

  21. microRNA analysis as the predictive tool of the breast cancer treatment

    SASANO Hironobu, ISHIDA Takanori, SUZUKI Takashi, MIKI Yasuhiro, ONO Katsuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

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    A new molecular technique as a breast cancer diagnosis is attracting a great deal of attention. In this research, I clarified relation between miRNA expression and pathological biomarkers using breast cancer tissues. These main results of this research are as follows: 1) LIN28 correlated with let-7 family 2) Identification of miRNAs relatedwith Ki-67, which is proliferation marker. and 3) Identification of miRNAs related with HER2, which is targeting molecule for breast cancer therapy. The results from our research suggest the possibility of the miRNA analysis as an novel diagnosis tool for breast cancer.

  22. The effects of sex steroid hormones on human pulmonary fibrosis

    MIKI Yasuhiro, ABE Keiko, SUZUKI Takashi, SASANO Hironobu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2010 - 2011

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    This is the first study to demonstrate tissue concentration of estrogen in human interstitial pneumonia (IP)/lung fibrosis tissues. Results from this study suggest that estradiol concentration in IP tissue is increased by aromatase, which is located in alveolar epithelial cells. In the result of mice model, expression level of phosphodiesterase mRNA in lung tissue was increased through the androgen signal. These results all suggest that sex steroid hormones, estrogen and androgen may play an important role in the development of IP.

  23. ヒト肝組織を用いたアクリルアミドの変異原性の評価とその関連遺伝子の解析

    Offer Organization: 内閣府食品安全委員会 食品健康影響評価技術研究

    2007/04 - 2010/03

  24. Lung cancer and Smoking : Effect of cigarette smoke extra on nuclear receptors

    MIKI Yasuhiro, ABE Keiko, SASANO Hironobu, SUSUKI Takashi, AKAHIRA Jun-ichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2008 - 2009

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    Recently, sex steroid hormones such as estrogen have been considered to play some roles in lung cancer. In this study, we demonstrated that cigarette smoke condensate (CSC) has estrogenic action through the estrogen receptor in vitro. In lung carcinoma patients, a correlation was found between the patients smoking history and CYP19 expression evaluated using quantitative RT-PCR. Results from our study suggested that CYP19 expression might be increased smoking through the cytokine signal. These findings all suggested that smoking might stimulate the estrogen signals directly and /or indirectly in lung carcinoma tissues.

  25. 公益財団法人中冨健康科学振興財団研究助成 骨組織の機能維持に対するエストロゲンの作用に関する研究

    Offer Organization: 公益財団法人中冨健康科学振興財団

    2007/04 - 2008/03

  26. 第22回基礎医学医療研究助成金 肺癌微小環境におけるアロマターゼの発現とその制御

    三木康宏

    Offer Organization: 財団法人金原一郎記念医学医療振興財団

    2007/04 - 2008/03

  27. Estrogen in human lung cancer-intratumoral hormone production and analysis of intracrine mechanisms-

    SASANO Hironobu, SUZUKI Satoshi, SUZUKI Takashi, HAYASHI Shin-ichi, MIKI Yasuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2006 - 2008

  28. 第39回医学研究助成 閉経後乳癌患者の骨粗鬆症治療に向けての基礎検討：骨細胞を用いたin vitroでのSERM誘導因子の検討

    Offer Organization: 財団法人三井生命厚生事業団

    2006/04 - 2007/03

  29. ヒト骨芽細胞におけるステロイドホルモンの影響:乳癌ホルモン療法の骨組織への影響

    三木 康宏

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東北大学

    2006 - 2007

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    骨組織においてはエストロゲンがその発育・維持に深く関与しており、selective estrogen receptor modulator(SERM)であるラロキシフェン(RAL)は、既に本邦においても骨粗雑症治療薬として用いられている。骨形成を担っている骨芽細胞に対するSERMの直接作用には不明な点が多い。本研究では、ヒト骨芽細胞に対するSERMの直接作用について、細胞増殖・周期及びマイクロアレイ解析を行い、エストロゲン(エストラジオール:E2)及びタモキシフェン(TAM)の作用との比較を行った。また、estrogenic compoundsとして知られているジエチルスチルベストロール(DES)、ゲニステイン(GEN)及びビスフェノールA(BPA)の影響も同様に検討した。ヒト骨芽細胞は正常細胞由来株のhFOB(ATCC)を用い、E2、TAM、RAL、DES、GEN及びBPAを添加し、細胞増殖(WST-8)に対する影響を調べた。次にマイクロアレイを用いてそれぞれの化合物を添加したときのhFOBの遺伝子発現解析を行い、階層的クラスター解析にてそれぞれの遺伝子発現プロファイルを比較した。さらに変動を示した遺伝子については、定量的PCRでのバリデーションを行った。E2、TAM及びRALの添加によって、いずれもhFOBの細胞数が増加した。また、BPAによる細胞増殖作用も確認されたが、GENでは変化は無く、DESはアポトーシスを引き起こした。階層的クラスター解析では、E2、TAM及びRAL添加時の全遺伝子での発現プロファイルにはほとんど差が認められなかったが、発現増加遺伝子群ではE2とRAL、発現低下遺伝子群ではE2とTAMの発現プロファイルがそれぞれ類似した。個々の遺伝子についてはRAL添加によって骨芽細胞の増殖に関連する遺伝子が誘導された。骨芽細胞に対し、SERMであるTAM及びRALはいずれも細胞増殖に働くことが示されたが、その作用に関連する遺伝子群には差異があることが考えられた。また、estrogenic compoundsに分類される化合物は、骨芽細胞に対して全く異なる作用を示した。

  30. 癌研究奨学金 ヒト乳癌細胞及び周囲間質細胞におけるアロマターゼの解析

    Offer Organization: 財団法人安田記念医学財団

    2005/04 - 2006/03

  31. 第9回研究助成金 閉経後乳癌患者の骨密度に対する内分泌療法剤の与える影響の基礎的研究

    三木康宏

    Offer Organization: 財団法人神澤医学研究振興財団

    2005/04 - 2006/03

  32. 第22回内分泌・代謝学サマーセミナー トラベルグラント

    三木康宏

    Offer Organization: 一般社団法人 日本内分泌学会

    2004/04 - 2005/03

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  1. エストロゲン依存性腫瘍における抗ホルモン療法耐性機序に関する研究

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    エストロゲン依存性腫瘍における抗ホルモン療法耐性機序に関する研究

  2. ストレスホルモンと子宮内膜癌：治療標的因子としてのコルチゾールシグナルの解明

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    ストレスホルモンと子宮内膜癌：治療標的因子としてのコルチゾールシグナルの解明

  3. ヒト乳癌細胞及び周囲間質細胞におけるアロマターゼの解析

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    ヒト乳癌細胞及び周囲間質細胞におけるアロマターゼの解析

  4. 閉経後乳癌患者の骨密度に対する内分泌療法剤の与える影響の基礎的研究

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    閉経後乳癌患者の骨密度に対する内分泌療法剤の与える影響の基礎的研究