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Hikaru Nakazawa

Section

Graduate School of Engineering

Job title

Associate Professor

Degree

博士（工学）（長岡技術科学大学）
修士（工学）（長岡技術科学大学）

researchmap

https://researchmap.jp/7000010851

J-GLOBAL ID

201501041481327630

Committee Memberships 4

生物工学会北日本支部　北日本支部幹事

2023/04 - Present
日本農芸化学会　産学官学術交流委員

2022/04 - Present
日本農芸化学会　産学官若手交流会さんわか

2017/04 - 2022/03
化学工学会化工誌編集委員会　化工誌編集委員

2017/04 - 2020/03

Professional Memberships 7

日本抗体学会

2022/10 - Present
日本化学会
農芸化学会
生物物理学会
化学工学会
蛋白質科学会
生物工学会

︎Show all ︎Show first 5

Research Interests 3

Bioengineering
Protein engineering
Enzyme engineering

Research Areas 1

Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering /

Awards 3

Poster award by the 3rd international Workshop by the 174th Committee JSPS “Symbiosis of Biology and Nanodevices (2019)

2019/08　the 174th Committee JSPS　Smart interface design with ZnO binding VHH for plasmonic biosensor
YABEC(Young Asian Biochemical Engineers’ Community) Poster Award

2013/08　AFOB
第27回セルラーゼ研究会, ポスター賞第一等,

2013/07　セルラーゼ研究会

Papers 62

Design of Cyborg Proteins by Loop Region Replacement with Oligo(ethylene glycol): Exploring Suitable Mutations for Cyborg Protein Construction Using Machine Learning Peer-reviewed

Wijak Yospanya, Akari Matsumura, Yukihiro Imasato, Tomoyuki Itou, Yusuke Aoki, Hikaru Nakazawa, Takashi Matsui, Takeshi Yokoyama, Mihoko Ui, Mitsuo Umetsu, Satoru Nagatoishi, Kouhei Tsumoto, Yoshikazu Tanaka, Kazushi Kinbara

Bulletin of the Chemical Society of Japan　2024/09/02
Publisher: Oxford University Press (OUP)
DOI： 10.1093/bulcsj/uoae090 　

ISSN： 0009-2673

eISSN： 1348-0634

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Abstract We synthesized a “cyborg protein,” wherein a synthetic molecule partially substitutes the main peptide chain by linking two protein domains with a synthetic oligomer. Green fluorescent protein (GFP) served as the model for constructing the cyborg proteins. We prepared circularly permuted GFP (cpGFP) with new termini between β10 and β11, where the original N- and C-termini were linked by a cleavable peptide loop. The cyborg GFP was constructed from cpGFP by linking the β10 and β11 with oligo(ethylene glycol) using maleimide-cysteine couplings, followed by the enzymatic cleavage of the N- and C-termini linking loop by thrombin. With the help of machine learning, we were able to obtain the cpGFP mutants that significantly alter the fluorescence activity (53% increase) by thrombin treatment, which splits cpGFP into two fragments (fragmented-GFP), and by heat shock. When the cyborg GFP was constructed using this mutant, the fluorescence intensity increased by 13% after heat treatment, similar to cpGFP (33% increase), and the behavior was significantly different from that of the fragmented-GFP. This result suggests the possibility that the oligo(ethylene glycol) chain in the cyborg protein plays a similar role to the peptide in the main chain of the protein.
Extensive antibody search with whole spectrum black-box optimization

Andrejs Tučs, Tomoyuki Ito, Yoichi Kurumida, Sakiya Kawada, Hikaru Nakazawa, Yutaka Saito, Mitsuo Umetsu, Koji Tsuda

Scientific Reports　14　(1)　2024/01/04
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41598-023-51095-z 　

eISSN： 2045-2322

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Abstract In designing functional biological sequences with machine learning, the activity predictor tends to be inaccurate due to shortage of data. Top ranked sequences are thus unlikely to contain effective ones. This paper proposes to take prediction stability into account to provide domain experts with a reasonable list of sequences to choose from. In our approach, multiple prediction models are trained by subsampling the training set and the multi-objective optimization problem, where one objective is the average activity and the other is the standard deviation, is solved. The Pareto front represents a list of sequences with the whole spectrum of activity and stability. Using this method, we designed VHH (Variable domain of Heavy chain of Heavy chain) antibodies based on the dataset obtained from deep mutational screening. To solve multi-objective optimization, we employed our sequence design software MOQA that uses quantum annealing. By applying several selection criteria to 19,778 designed sequences, five sequences were selected for wet-lab validation. One sequence, 16 mutations away from the closest training sequence, was successfully expressed and found to possess desired binding specificity. Our whole spectrum approach provides a balanced way of dealing with the prediction uncertainty, and can possibly be applied to extensive search of functional sequences.
Recent research advances on non-linear phenomena in various biosystems

Yutaka Tamaru, Shuji Nakanishi, Kenya Tanaka, Mitsuo Umetsu, Hikaru Nakazawa, Aruto Sugiyama, Tomoyuki Ito, Naofumi Shimokawa, Masahiro Takagi

Journal of Bioscience and Bioengineering　136　(2)　75-86　2023/08
Publisher: Elsevier BV
DOI： 10.1016/j.jbiosc.2023.03.012 　

ISSN： 1389-1723
Synthesis of epitope-targeting nanobody based on native protein-protein interactions for FtsZ filamentation suppressor. International-journal

Hikaru Nakazawa, Taiji Katsuki, Takashi Matsui, Atsushi Tsugita, Takeshi Yokoyama, Tomoyuki Ito, Sakiya Kawada, Yoshikazu Tanaka, Mitsuo Umetsu

Biotechnology journal　e2300039　2023/07/17

DOI： 10.1002/biot.202300039 　

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Phage display and biopanning are powerful tools for generating binding molecules for a specific target. However, the selection process based only on binding affinity provides no assurance for the antibody's affinity to the target epitope. In this study, we propose a molecular-evolution approach guided by native protein-protein interactions to generate epitope-targeting antibodies. The binding-site sequence in a native protein was grafted into a complementarity-determining region (CDR) in the nanobody, and a nonrelated CDR loop (in the grafted nanobody) was randomized to create a phage display library. In this construction of nanobodies by integrating graft and evolution technology (CAnIGET method), suitable grafting of the functional sequence added functionality to the nanobody, and the molecular-evolution approach enhanced the binding function to inhibit the native protein-protein interactions. To apply for biological tool with growth screening, model nanobodies with an affinity for filamenting temperature-sensitive mutant Z (FtsZ) from Staphylococcus aureus were constructed and completely inhibited the polymerization of FtsZ as a function. Consequently, the expression of these nanobodies drastically decreased the cell division rate. We demonstrate the potential of the CAnIGET method with the use of native protein-protein interactions for steady epitope-specific evolutionary engineering. This article is protected by copyright. All rights reserved.
Selection of target-binding proteins from the information of weakly enriched phage display libraries by deep sequencing and machine learning. International-journal

Tomoyuki Ito, Thuy Duong Nguyen, Yutaka Saito, Yoichi Kurumida, Hikaru Nakazawa, Sakiya Kawada, Hafumi Nishi, Koji Tsuda, Tomoshi Kameda, Mitsuo Umetsu

mAbs　15　(1)　2168470-2168470　2023

DOI： 10.1080/19420862.2023.2168470 　

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Despite the advances in surface-display systems for directed evolution, variants with high affinity are not always enriched due to undesirable biases that increase target-unrelated variants during biopanning. Here, our goal was to design a library containing improved variants from the information of the "weakly enriched" library where functional variants were weakly enriched. Deep sequencing for the previous biopanning result, where no functional antibody mimetics were experimentally identified, revealed that weak enrichment was partly due to undesirable biases during phage infection and amplification steps. The clustering analysis of the deep sequencing data from appropriate steps revealed no distinct sequence patterns, but a Bayesian machine learning model trained with the selected deep sequencing data supplied nine clusters with distinct sequence patterns. Phage libraries were designed on the basis of the sequence patterns identified, and four improved variants with target-specific affinity (EC50 = 80-277 nM) were identified by biopanning. The selection and use of deep sequencing data without undesirable bias enabled us to extract the information on prospective variants. In summary, the use of appropriate deep sequencing data and machine learning with the sequence data has the possibility of finding sequence space where functional variants are enriched.
G6P-capturing molecules in the periplasm of Escherichia coli accelerate the shikimate pathway International-journal

Ryosuke Fujiwara, Mariko Nakano, Yuuki Hirata, Chisako Otomo, Daisuke Nonaka, Sakiya Kawada, Hikaru Nakazawa, Mitsuo Umetsu, Tomokazu Shirai, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

Metabolic Engineering　72　68-81　2022/07
Publisher: Elsevier BV
DOI： 10.1016/j.ymben.2022.03.002 　

ISSN： 1096-7176

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Escherichia coli, the most studied prokaryote, is an excellent host for producing valuable chemicals from renewable resources as it is easy to manipulate genetically. Since the periplasmic environment can be easily controlled externally, elucidating how the localization of specific proteins or small molecules in the periplasm affects metabolism may lead to bioproduction development using E. coli. We investigated metabolic changes and its mechanisms occurring when specific proteins are localized to the E. coli periplasm. We found that the periplasmic localization of β-glucosidase promoted the shikimate pathway involved in the synthesis of aromatic chemicals. The periplasmic localization of other proteins with an affinity for glucose-6-phosphate (G6P), such as inactivated mutants of Pgi, Zwf, and PhoA, similarly accelerated the shikimate pathway. Our results indicate that G6P is transported from the cytoplasm to the periplasm by the glucose transporter protein EIICBGlc, and then captured by β-glucosidase.
A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii Peer-reviewed

Yoichiro Ito, Misa Ishigami, Goro Terai, Yasuyuki Nakamura, Noriko Hashiba, Teruyuki Nishi, Hikaru Nakazawa, Tomohisa Hasunuma, Kiyoshi Asai, Mitsuo Umetsu, Jun Ishii, Akihiko Kondo

Communications Biology　5　(1)　561　2022/06
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s42003-022-03475-w 　

eISSN： 2399-3642

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Abstract Expression of secreted recombinant proteins burdens the protein secretion machinery, limiting production. Here, we describe an approach to improving protein production by the non-conventional yeast Komagataella phaffii comprised of genome-wide screening for effective gene disruptions, combining them in a single strain, and recovering growth reduction by adaptive evolution. For the screen, we designed a multiwell-formatted, streamlined workflow to high-throughput assay of secretion of a single-chain small antibody, which is cumbersome to detect but serves as a good model of proteins that are difficult to secrete. Using the consolidated screening system, we evaluated >19,000 mutant strains from a mutant library prepared by a modified random gene-disruption method, and identified six factors for which disruption led to increased antibody production. We then combined the disruptions, up to quadruple gene knockouts, which appeared to contribute independently, in a single strain and observed an additive effect. Target protein and promoter were basically interchangeable for the effects of knockout genes screened. We finally used adaptive evolution to recover reduced cell growth by multiple gene knockouts and examine the possibility for further enhancing protein secretion. Our successful, three-part approach holds promise as a method for improving protein production by non-conventional microorganisms.
Enzymatic ligation of an antibody and arginine 9 peptide for efficient and cell-specific siRNA delivery

Yu Ando, Hikaru Nakazawa, Daisuke Miura, Maho Otake, Mitsuo Umetsu

Scientific Reports　11　(1)　2021/12
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41598-021-01331-1 　

eISSN： 2045-2322

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Abstract A fusion protein comprising an antibody and a cationic peptide, such as arginine-9 (R9), is a candidate molecule for efficient and cell-specific delivery of siRNA into cells in order to reduce the side effects of nucleic acid drugs. However, their expression in bacterial hosts, required for their development, often fails, impeding research progress. In this study, we separately prepared anti-EGFR nanobodies with the K-tag sequence MRHKGS at the C-terminus and R9 with the Q-tag sequence LLQG at the N-terminus, and enzymatically ligated them in vitro by microbial transglutaminase to generate Nanobody-R9, which is not expressed as a fused protein in E. coli. Nanobody-R9 was synthesized at a maximum binding efficiency of 85.1%, without changing the binding affinity of the nanobody for the antigen. Nanobody-R9 successfully delivered siRNA into the cells, and the cellular influx of siRNA increased with increase in the ratio of Nanobody-R9 to siRNA. We further demonstrated that the Nanobody-R9–siRNA complex, at a 30:1 ratio, induced an approximately 58.6% reduction in the amount of target protein due to RNAi in mRNA compared to lipofectamine.
Machine-Learning-Guided Library Design Cycle for Directed Evolution of Enzymes: The Effects of Training Data Composition on Sequence Space Exploration Peer-reviewed

Yutaka Saito, Misaki Oikawa, Takumi Sato, Hikaru Nakazawa, Tomoyuki Ito, Tomoshi Kameda, Koji Tsuda, Mitsuo Umetsu

ACS Catalysis　11　(23)　14615-14624　2021/12

DOI： 10.1021/acscatal.1c03753 　

ISSN： 2155-5435
Combination Informatic and Experimental Approach for Selecting Scaffold Proteins for Development as Antibody Mimetics

Ito Tomoyuki, Nishi Hafumi, Kameda Tomoshi, Yoshida Mayu, Fukazawa Reito, Kawada Sakiya, Nakazawa Hikaru, Umetsu Mitsuo

Chemistry Letters　50　(11)　1867-1871　2021
Publisher: The Chemical Society of Japan
DOI： 10.1246/cl.210443 　

ISSN： 0366-7022

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<p>Antibody mimetics are proteins smaller than antibodies that have antigen-binding properties. Here, we used a combination of informatic selection and experimental verification to identify from a public database several candidate scaffold proteins. From those candidates, we selected a protein with high thermal stability, bacterial expression, and mutation tolerance and used molecular engineering and phage display selection techniques to develop the scaffold into an antibody mimetic with binding affinity for galactin-3, a biomarker of cancer and heart disease.</p>
Association behavior and control of the quality of cancer therapeutic bispecific diabodies expressed in Escherichia coli Peer-reviewed

Hikaru Nakazawa, Tomoko Onodera-Sugano, Aruto Sugiyama, Yoshikazu Tanaka, Takamitsu Hattori, Teppei Niide, Hiromi Ogata, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu

Biochemical Engineering Journal　160　107636-107636　2020/08/15
Publisher: Elsevier BV
DOI： 10.1016/j.bej.2020.107636 　

ISSN： 1369-703X

eISSN： 1873-295X
Chemically Crosslinked Bispecific Antibodies for Cancer Therapy: Breaking from the Structural Restrictions of the Genetic Fusion Approach. International-journal Peer-reviewed

Asami Ueda, Mitsuo Umetsu, Takeshi Nakanishi, Kentaro Hashikami, Hikaru Nakazawa, Shuhei Hattori, Ryutaro Asano, Izumi Kumagai

International journal of molecular sciences　21　(3)　2020/01/21

DOI： 10.3390/ijms21030711 　

ISSN： 1661-6596

eISSN： 1422-0067
Identification of Indium Tin Oxide Nanoparticle-Binding Peptides via Phage Display and Biopanning Under Various Buffer Conditions. International-journal Peer-reviewed

Hikaru Nakazawa, Mitsuo Umetsu, Tatsuya Hirose, Takamitsu Hattori, Izumi Kumagai

Protein and peptide letters　27　(6)　557-566　2020

DOI： 10.2174/0929866526666191113151934 　

ISSN： 0929-8665

eISSN： 1875-5305
Nanoparticle Assisted Remodeling of Proteotoxic SOD1 Mutants Alters the Biointerface of the Functional Interaction of Microtubules and Kinesin Motors Peer-reviewed

Kyongwan Kim, Selvaraj Subramaniyam, Ahmad Galaleldeen, Hikaru Nakazawa, Mitsuo Umetsu, Winfried Teizer, Sanjib Bhattacharyya

ACS Applied Bio Materials　2　(10)　4121-4128　2019/10/21

DOI： 10.1021/acsabm.9b00501 　

eISSN： 2576-6422
抗体断片の熱安定性向上を目的としたバイオ情報を活用したライブラリーデザインサイクルの提案

服部修平, 本田亜由美, 二井手哲平, 中澤光, 梅津光央

日本生物工学会大会講演要旨集　2019年　280-280　2019/08
Publisher: (公社)日本生物工学会
Complementary Design for Pairing between Two Types of Nanoparticles Mediated by a Bispecific Antibody: Bottom-Up Formation of Porous Materials from Nanoparticles. International-journal Peer-reviewed

Teppei Niide, Noriyoshi Manabe, Hikaru Nakazawa, Kazuto Akagi, Takamitsu Hattori, Izumi Kumagai, Mitsuo Umetsu

Langmuir : the ACS journal of surfaces and colloids　35　(8)　3067-3076　2019/02/26

DOI： 10.1021/acs.langmuir.8b03687 　

ISSN： 0743-7463

eISSN： 1520-5827
Machine-Learning-Guided Mutagenesis for Directed Evolution of Fluorescent Proteins. International-journal Peer-reviewed

Yutaka Saito, Misaki Oikawa, Hikaru Nakazawa, Teppei Niide, Tomoshi Kameda, Koji Tsuda, Mitsuo Umetsu

ACS synthetic biology　7　(9)　2014-2022　2018/09/21

DOI： 10.1021/acssynbio.8b00155 　

ISSN： 2161-5063

eISSN： 2161-5063
抗体データベースを利用した熱安定性向上と発現量向上に向けた大規模変異導入プロセスの開発

服部修平, 本田亜由美, 二井手哲平, 中澤光, 梅津光央

日本生物工学会大会講演要旨集　平成30年度　157-157　2018/08
Publisher: (公社)日本生物工学会
High-throughput cytotoxicity and antigen-binding assay for screening small bispecific antibodies without purification. Peer-reviewed

Aruto Sugiyama, Mitsuo Umetsu, Hikaru Nakazawa, Teppei Niide, Ryutaro Asano, Takamitsu Hattori, Izumi Kumagai

Journal of bioscience and bioengineering　126　(2)　153-161　2018/08

DOI： 10.1016/j.jbiosc.2018.02.007 　

ISSN： 1389-1723

eISSN： 1347-4421
Impact in stability during sequential CDR grafting to construct camelid VHH antibodies against zinc oxide and gold. International-journal Peer-reviewed

Ryota Saito, Yutaro Saito, Hikaru Nakazawa, Takamitsu Hattori, Izumi Kumagai, Mitsuo Umetsu, Koki Makabe

Journal of biochemistry　164　(1)　21-25　2018/07/01

DOI： 10.1093/jb/mvy016 　

ISSN： 0021-924X

eISSN： 1756-2651
Compact Seahorse-Shaped T Cell-Activating Antibody for Cancer Therapy Peer-reviewed

Fujii, H, Tanaka, Y, Nakazawa, H, Sugiyama, A, Manabe, N, Shinoda, A, Shimizu, N, Hattori, T, Hosokawa, K, Sujino, T, Ito, T, Niide, T, Asano, R, Kumagai, I, Umetsu, M

Adv. Therapeut.　1　(1700031)　1700031-1700031　2018/06
Publisher: Wiley
DOI： 10.1002/adtp.201700031 　

ISSN： 2366-3987

eISSN： 2366-3987
Large-scale chirality in an active layer of microtubules and kinesin motor proteins. International-journal Peer-reviewed

Kyongwan Kim, Natsuhiko Yoshinaga, Sanjib Bhattacharyya, Hikaru Nakazawa, Mitsuo Umetsu, Winfried Teizer

Soft matter　14　(17)　3221-3231　2018/05/02
Publisher: Royal Society of Chemistry
DOI： 10.1039/c7sm02298k 　

ISSN： 1744-683X

eISSN： 1744-6848
Use of a Phage-Display Method to Identify Peptides that Bind to a Tin Oxide Nanosheets. International-journal Peer-reviewed

Hikaru Nakazawa, Yasuko Seta, Tatsuya Hirose, Yoshitake Masuda, Mitsuo Umetsu

Protein and peptide letters　25　(1)　68-75　2018
Publisher: Bentham Science Publishers B.V.
DOI： 10.2174/0929866525666171206114429 　

ISSN： 0929-8665

eISSN： 1875-5305
A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity. International-journal Peer-reviewed

Aruto Sugiyama, Mitsuo Umetsu, Hikaru Nakazawa, Teppei Niide, Tomoko Onodera, Katsuhiro Hosokawa, Shuhei Hattori, Ryutaro Asano, Izumi Kumagai

Scientific reports　7　(1)　2862-2862　2017/06/06

DOI： 10.1038/s41598-017-03101-4 　

ISSN： 2045-2322

eISSN： 2045-2322
Combination of molecular evolution and conformational information for antibody mimic

Umetsu Mitsuo, Nakazawa Hikaru, Niide Teppei

MEDCHEM NEWS　27　(1)　25-29　2017/02/01
Publisher: The Pharmaceutical Society of Japan
DOI： 10.14894/medchem.27.1_25 　

ISSN： 2432-8618

eISSN： 2432-8626

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Advance in library approach and accumulation of conformational information of proteins are causing drastic changes of protein engineering. Flexible design for the combination of amino acids appearing in library and easy access to conformational information enable us to draw up effective and dense libraries to express a new function on non-functional protein. Here, we show the creation of a protein with a new function, especially molecular recognition, by means of library approach and conformational information of proteins.
親和性ペプチド分子による有機結晶の構造制御と生体分子-半導体有機結晶ハイブリッド材料作製プロセスの開発

二井手哲平, 中澤光, 梅津光央

ケミカルエンジニヤリング　62　23-27　2017
Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing. International-journal Peer-reviewed

Yuki Shibuya, Natsuki Haga, Ryutaro Asano, Hikaru Nakazawa, Takamitsu Hattori, Daisuke Takeda, Aruto Sugiyama, Reiko Kurotani, Izumi Kumagai, Mitsuo Umetsu, Koki Makabe

Protein engineering, design & selection : PEDS　30　(1)　15-21　2017/01

DOI： 10.1093/protein/gzw057 　

ISSN： 1741-0126

eISSN： 1741-0134
Modulating the microtubule-tau interactions in biomotility systems by altering the chemical environment Peer-reviewed

S. Bhattacharyya, K. Kim, H. Nakazawa, M. Umetsu, W. Teizer

Integrative Biology (United Kingdom)　8　(12)　1296-1300　2016/12

DOI： 10.1039/c6ib00182c 　

ISSN： 1757-9694

eISSN： 1757-9708
Altering the microtubule kinesin nanomechanics in the presence of metallic ions Peer-reviewed

Sanjib Bhattacharyya, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Winfried Teizer

16th International Conference on Nanotechnology - IEEE NANO 2016　79-81　2016/11/21

DOI： 10.1109/NANO.2016.7751410 　
Salt-Switchable Artificial Cellulase Regulated by a DNA Aptamer. International-journal Peer-reviewed

Mari Takahara, Geisa Aparecida Lopes Gonçalves Budinova, Hikaru Nakazawa, Yutaro Mori, Mitsuo Umetsu, Noriho Kamiya

Biomacromolecules　17　(10)　3356-3362　2016/10/10

DOI： 10.1021/acs.biomac.6b01141 　

ISSN： 1525-7797

eISSN： 1526-4602
Contributions of loop histidine residues for Zinc ion binding and its stability of an anti-ZnO VHH antibody Peer-reviewed

Ryosuke Sasaki, Hikaru Nakazawa, Izumi Kumagai, Mitsuo Umetsu, Koki Makabe

PROTEIN SCIENCE　25　(S1)　168-168　2016/10

ISSN： 0961-8368

eISSN： 1469-896X
Isomorphic coalescence of aster cores formed in vitro from microtubules and kinesin motors Peer-reviewed

K. Kim, A. Sikora, H. Nakazawa, M. Umetsu, W. Hwang, W. Teizer

Physical Biology　13　(5)　2016/09/21

DOI： 10.1088/1478-3975/13/5/056002 　

ISSN： 1478-3967

eISSN： 1478-3975
Interleukin-6 Detection with a Plasmonic Chip Peer-reviewed

Keiko Tawa, Masashi Sumiya, Mana Toma, Chisato Sasakawa, Takuma Sujino, Tatsuki Miyaki, Hikaru Nakazawa, Mitsuo Umetsu

JOURNAL OF MOLECULAR AND ENGINEERING MATERIALS　4　(3)　1640009　2016/09

DOI： 10.1142/S2251237316400098 　

ISSN： 2251-2373

eISSN： 2251-2381
One-dimensional assembly of functional proteins: Toward the design of an artificial cellulosome Peer-reviewed

Y. Mori, H. Nakazawa, G. A.L. Gonçalves, T. Tanaka, M. Umetsu, N. Kamiya

Molecular Systems Design and Engineering　1　(1)　66-73　2016

DOI： 10.1039/c5me00011d 　

eISSN： 2058-9689
A high performance Trichoderma reesei strain that reveals the importance of xylanase III in cellulosic biomass conversion. International-journal Peer-reviewed

Hikaru Nakazawa, Tetsushi Kawai, Noriko Ida, Yosuke Shida, Kouki Shioya, Yoshinori Kobayashi, Hirofumi Okada, Shuji Tani, Jun-Ichi Sumitani, Takashi Kawaguchi, Yasushi Morikawa, Wataru Ogasawara

Enzyme and microbial technology　82　89-95　2016/01

DOI： 10.1016/j.enzmictec.2015.08.019 　

ISSN： 0141-0229

eISSN： 1879-0909
Heterologously expressed Aspergillus aculeatus β-glucosidase in Saccharomyces cerevisiae is a cost-effective alternative to commercial supplementation of β-glucosidase in industrial ethanol production using Trichoderma reesei cellulases. Peer-reviewed

Treesukon Treebupachatsakul, Hikaru Nakazawa, Hideaki Shinbo, Hiroki Fujikawa, Asami Nagaiwa, Nobuhiro Ochiai, Takashi Kawaguchi, Mitsuru Nikaido, Kazuhide Totani, Koki Shioya, Yosuke Shida, Yasushi Morikawa, Wataru Ogasawara, Hirofumi Okada

Journal of bioscience and bioengineering　121　(1)　27-35　2016/01

DOI： 10.1016/j.jbiosc.2015.05.002 　

ISSN： 1389-1723

eISSN： 1347-4421
Characterization of two endoglucanases for the classification of the earthworm, Eisenia fetida Waki. International-journal Peer-reviewed

Shin-ichi Akazawa, Yuki Ikarashi, Jun Yarimizu, Keisuke Yokoyama, Tomoya Kobayashi, Hikaru Nakazawa, Wataru Ogasawara, Yasushi Morikawa

Bioscience, biotechnology, and biochemistry　80　(1)　55-66　2016

DOI： 10.1080/09168451.2015.1075860 　

ISSN： 0916-8451

eISSN： 1347-6947
Organic crystal-binding peptides: morphology control and one-pot formation of protein-displaying organic crystals. International-journal Peer-reviewed

Teppei Niide, Kyohei Ozawa, Hikaru Nakazawa, Daniel Oliveira, Hitoshi Kasai, Mari Onodera, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu

Nanoscale　7　(47)　20155-63　2015/12/21

DOI： 10.1039/c5nr06471f 　

ISSN： 2040-3364

eISSN： 2040-3372
Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus β-glucosidase I (JN11) for a more economical production of ethanol from lignocellulosic biomass. Peer-reviewed

Treesukon Treebupachatsakul, Koki Shioya, Hikaru Nakazawa, Takashi Kawaguchi, Yasushi Morikawa, Yosuke Shida, Wataru Ogasawara, Hirofumi Okada

Journal of bioscience and bioengineering　120　(6)　657-65　2015/12

DOI： 10.1016/j.jbiosc.2015.04.015 　

ISSN： 1389-1723

eISSN： 1347-4421
Behavior of Kinesin Driven Quantum Dots Trapped in a Microtubule Loop. International-journal Peer-reviewed

Aurélien Sikora, Filippo Federici Canova, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Wonmuk Hwang, Winfried Teizer

ACS nano　9　(11)　11003-13　2015/11/24

DOI： 10.1021/acsnano.5b04348 　

ISSN： 1936-0851

eISSN： 1936-086X
Zinc Ion-binding Activity of an Anti-ZnO VHH Antibody, 4F2 Peer-reviewed

Ryosuke Sasaki, Soichiro Kitazawa, Ryo Kitahara, Hikaru Nakazawa, Yoshikazu Tanaka, Izumi Kumagai, Mitsuo Umetsu, Koki Makabe

CHEMISTRY LETTERS　44　(10)　1309-1311　2015/10

DOI： 10.1246/cl.150537 　

ISSN： 0366-7022

eISSN： 1348-0715
Microtubule guiding in a multi-walled carbon nanotube circuit Peer-reviewed

Aurélien Sikora, Javier Ramón-Azcón, Mustafa Sen, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Hitoshi Shiku, Tomokazu Matsue, Winfried Teizer

Biomedical Microdevices　17　(4)　2015/08
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s10544-015-9978-1 　

ISSN： 1387-2176

eISSN： 1572-8781
Microtubule guiding in a multi-walled carbon nanotube circuit. International-journal Peer-reviewed

Aurélien Sikora, Javier Ramón-Azcón, Mustafa Sen, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Hitoshi Shiku, Tomokazu Matsue, Winfried Teizer

Biomedical microdevices　17　(4)　78-78　2015/08

DOI： 10.1007/s10544-015-9978-1 　

ISSN： 1387-2176

eISSN： 1572-8781
ナノの発想からの新しい酵素複合体設計：ハイブリッドナノセルロソーム

梅津光央, 中澤光

酵素工学ニュース　72　10-14　2014/10
Publisher: 酵素工学研究会
Functional localization of kinesin/microtubule-based motility system along metallic glass microwires Peer-reviewed

K. Kim, A. Sikora, K. S. Nakayama, H. Nakazawa, M. Umetsu, W. Hwang, W. Teizer

APPLIED PHYSICS LETTERS　105　(14)　1-5　2014/10

DOI： 10.1063/1.4896964 　

ISSN： 0003-6951

eISSN： 1077-3118
Microtubule shuttles on kinesin-coated glass micro-wire tracks. International-journal Peer-reviewed

Kyongwan Kim, Andrew L Liao, Aurélien Sikora, Daniel Oliveira, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Wonmuk Hwang, Winfried Teizer

Biomedical microdevices　16　(4)　501-8　2014/08

DOI： 10.1007/s10544-014-9852-6 　

ISSN： 1387-2176

eISSN： 1572-8781
Molecular motor-powered shuttles along multi-walled carbon nanotube tracks. International-journal Peer-reviewed

Aurélien Sikora, Javier Ramón-Azcón, Kyongwan Kim, Kelley Reaves, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Hitoshi Shiku, Tomokazu Matsue, Wonmuk Hwang, Winfried Teizer

Nano letters　14　(2)　876-81　2014/02/12

DOI： 10.1021/nl4042388 　

ISSN： 1530-6984

eISSN： 1530-6992
Detection of Brain-Derived Neurotrophic Factor (BDNF) with a Sandwich Assay on a Plasmonic Chip

Mari Satoh, Keiko Tawa, Koichi Uegaki, Tomoko Hara, Mitsuo Umetsu, Hikaru Nakazawa, Makoto Itakura, Masami Takahashi, Hiroyuki Aota, Masami Kojima

Transactions of the Materials Research Society of Japan　39　(3)　361-364　2014
Publisher: The Materials Research Society of Japan
DOI： 10.14723/tmrsj.39.361 　

ISSN： 1382-3469

eISSN： 2188-1650
2P086 Smart interface antibody design for biosensor(01F. Protein: Engineering,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Sujino Takuma, Nakazawa Hikaru, Tawa Keiko, Asano Ryutaro, Kumagai Izumi, Umetsu Mitsuo

Seibutsu Butsuri　54　(1)　S209　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S209_2 　
Application of 300x Enhanced Fluorescence on a Plasmonic Chip Modified with a Bispecific Antibody to a Sensitive lmmunosensor Peer-reviewed

Keiko Tawa, Mitsuo Umetsu, Hikaru Nakazawa, Takamitsu Hattori, Izumi Kumagai

ACS APPLIED MATERIALS & INTERFACES　5　(17)　8628-8632　2013/09

DOI： 10.1021/am402173y 　

ISSN： 1944-8244
A comprehensive analysis of the effects of the main component enzymes of cellulase derived from Trichoderma reesei on biomass saccharification Peer-reviewed

Tetsushi Kawai, Hikaru Nakazawa, Noriko Ida, Hirofumi Okada, Wataru Ogasawara, Yasushi Morikawa, Yoshinori Kobayashi

Journal of Industrial Microbiology and Biotechnology　40　(8)　805-810　2013/08

DOI： 10.1007/s10295-013-1290-6 　

ISSN： 1367-5435 1476-5535
Rapid and Sensitive Detection of Brain-Derived Neurotrophic Factor with a Plasmonic Chip Peer-reviewed

Keiko Tawa, Mari Satoh, Koichi Uegaki, Tomoko Hara, Masami Kojima, Haruko Kumanogoh, Hiroyuki Aota, Yoshiki Yokota, Takahiko Nakaoki, Mitsuo Umetsu, Hikaru Nakazawa, Izumi Kumagai

JAPANESE JOURNAL OF APPLIED PHYSICS　52　(6)　UNSP 06GK01　2013/06

DOI： 10.7567/JJAP.52.06GK01 　

ISSN： 0021-4922

eISSN： 1347-4065
2P089 Evolutional cellulosome design from module library(01F. Protein: Engineering,Poster)

Nakazawa Hikaru, Ishigaki Yuri, Kobayashi Eiko, Kim Do-Myoung, Umetsu Mitsuo

Seibutsu Butsuri　53　(1)　S173　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S173_5 　
Biomass-binding peptides designed by molecular evolution for efficient degradation of cellulose in biomass by cellulase Peer-reviewed

Hikaru Nakazawa, Akinori Ikeuchi, Do-Myoung Kim, Yuri Ishigaki, Hidetaka Asano, Katsunori Kouda, Izumi Kumagai, Mitsuo Umetsu

GREEN CHEMISTRY　15　(2)　365-369　2013

DOI： 10.1039/c2gc36914a 　

ISSN： 1463-9262
In-one-pot-at-a-time ligation for high-throughput construction of a protein expression vector library Peer-reviewed

Hikaru Nakazawa, Rui Todokoro, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu

Chemistry Letters　42　(4)　424-426　2013

DOI： 10.1246/cl.130014 　

ISSN： 0366-7022 1348-0715
Hybrid nanocellulosome design from cellulase modules on nanoparticles: Synergistic effect of catalytically divergent cellulase modules on cellulose degradation activity Peer-reviewed

Hikaru Nakazawa, Do-Myoung Kim, Takashi Matsuyama, Nobuhiro Ishida, Akinori Ikeuchi, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu

ACS Catalysis　3　(6)　1342-1348　2013

DOI： 10.1021/cs400012v 　

ISSN： 2155-5435
Analysis of the saccharification capability of high-functional cellulase JN11 for various pretreated biomasses through a comparison with commercially available counterparts Peer-reviewed

Tetsushi Kawai, Hikaru Nakazawa, Noriko Ida, Hirofumi Okada, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi, Wataru Ogasawara, Yasushi Morikawa, Yoshinori Kobayashi

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY　39　(12)　1741-1749　2012/12

DOI： 10.1007/s10295-012-1195-9 　

ISSN： 1367-5435
1C1510 Hybrid nano-cellulosome: functional analysis of various cellulose binding module as component of artificial cellulosome(Proteins: Measurement, Analysis, Engineering,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

Nakazawa Hikaru, Kim Do-myoung, Matuyama Takashi, Ishida Nobuhiro, Ikeuchi Akinori, Kumagai Izumi, Umetsu Mitsuo

Seibutsu Butsuri　52　S23-S24　2012
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.52.S23_5 　
A nanocluster design for the construction of artificial cellulosomes Peer-reviewed

Do-Myoung Kim, Hikaru Nakazawa, Mitsuo Umetsu, Takashi Matsuyama, Nobuhiro Ishida, Akinori Ikeuchi, Haruo Takahashi, Ryutaro Asano, Izumi Kumagai

CATALYSIS SCIENCE & TECHNOLOGY　2　(3)　499-503　2012

DOI： 10.1039/c2cy00371f 　

ISSN： 2044-4753
Construction of a recombinant Trichoderma reesei strain expressing Aspergillus aculeatus ss-glucosidase 1 for efficient biomass conversion Peer-reviewed

Hikaru Nakazawa, Tetsushi Kawai, Noriko Ida, Yosuke Shida, Yoshinori Kobayashi, Hirofumi Okada, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi, Yasushi Morikawa, Wataru Ogasawara

BIOTECHNOLOGY AND BIOENGINEERING　109　(1)　92-99　2012/01

DOI： 10.1002/bit.23296 　

ISSN： 0006-3592

eISSN： 1097-0290
Directed evolution of endoglucanase III (Cel12A) from Trichoderma reesei Peer-reviewed

Hikaru Nakazawa, Katsunori Okada, Tomoko Onodera, Wataru Ogasawara, Hirofumi Okada, Yasushi Morikawa

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　83　(4)　649-657　2009/06

DOI： 10.1007/s00253-009-1901-3 　

ISSN： 0175-7598

eISSN： 1432-0614
Characterization of the catalytic domains of Trichoderma reesei endoglucanase I, II, and III, expressed in Escherichia coli Peer-reviewed

Hikaru Nakazawa, Katsunori Okada, Ryota Kobayashi, Tetsuya Kubota, Tomoko Onodera, Nobuhiro Ochiai, Naoki Omata, Wataru Ogasawara, Hirofumi Okada, Yasushi Morikawa

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　81　(4)　681-689　2008/12

DOI： 10.1007/s00253-008-1667-z 　

ISSN： 0175-7598

eISSN： 1432-0614

Show all ︎Show first 5

Misc. 59

光-熱変換ナノ粒子を用いた太陽光応答型酵素活性化デバイス

中澤光, 二井手哲平, 石垣友理, 梅津光央

日本蛋白質科学会年会(Web)　24th　2024
FtsZのネイティブタンパク質間相互作用から発想するエピトープターゲティング抗体設計

中澤光, 勝木泰司, 松井崇, 田中良和, 梅津光央

日本抗体学会学術大会プログラム・抄録集(Web)　2nd　2023
Synthesis of epitope-targeting antibody based on native Protein-Protein interaction

中澤光, 勝木泰司, 松井崇, 田中良和, 梅津光央

日本生物工学会大会講演要旨集　75th　2023
細胞選択的RNAiを誘発する抗体-膜貫通ペプチド酵素的架橋設計

中澤光, 勝木泰司, 松井崇, 田中良和, 梅津光央

日本抗体学会学術大会プログラム・抄録集(Web)　1st　2022
FtsZのネイティブタンパク質間相互作用から発想するエピトープターゲティング抗体設計

中澤光, 勝木泰司, 松井崇, 田中良和, 梅津光央

化学工学会秋季大会研究発表講演要旨集(CD-ROM)　53rd　2022
Hybrid Nanoassembly via Material-binding Peptides and Proteins

梅津光央, 中澤光, 二井手哲平

Bio Industry　39　(3)　2022

ISSN： 0910-6545
Application of Next-Generation Sequencing Analysis in the Directed Evolution for Creating Antibody Mimic

Tomoyuki Ito, Hafumi Nishi, Thuy Duong Nguyen, Yutaka Saito, Tomoshi Kameda, Hikaru Nakazawa, Koji Tsuda, Mitsuo Umetsu

BIOPHYSICAL JOURNAL　120　(3)　87A-87A　2021/02

ISSN： 0006-3495

eISSN： 1542-0086
そのエピトープは創薬標的となりえるか?:天然タンパク質の相互作用情報を利用したツール抗体設計

勝木泰司, 中澤光, 松井崇, 田中良和, 梅津光央

化学工学会秋季大会研究発表講演要旨集(CD-ROM)　51st　2020
MACHINE-LEARNING-GUIDED MUTAGENESIS FOR DIRECTED EVOLUTION OF FLUORESCENT PROTEINS

Tomoshi Kameda, Yutaka Saito, Misaki Oikawa, Hikaru Nakazawa, Teppei Niide, Koji Tsuda, Mitsuo Umetsu

PROTEIN SCIENCE　28　203-204　2019/09

ISSN： 0961-8368

eISSN： 1469-896X
機械学習支援によるタンパク質進化工学検証:GFPからYFPへ

及川未早来, 齋藤裕, 亀田倫史, 中澤光, 二井手哲平, 津田宏治, 梅津光央

化学工学会年会研究発表講演要旨集(CD-ROM)　84th　2019
ライブラリーデザインサイクルによる臨床試験と動物実験を結ぶための種交差性抗体の作製

服部修平, 服部峰充, 本田亜由美, 二井手哲平, 中澤光, 山口純奈, 西裕志, 梅津光央

日本細胞生物学会大会(Web)　71st　2019
AIが導くタンパク質の進化:指向性が強い変異体は必要か?

及川未早来, 齋藤裕, 齋藤裕, 亀田倫史, 亀田倫史, 中澤光, 二井手哲平, 津田宏治, 津田宏治, 梅津光央, 梅津光央

日本細胞生物学会大会(Web)　71st　2019
抗体の配列柔軟性を利用した部分構造移植による機能性タンパク質の創出

阿久津澪, 中澤光, 二井手哲平, 亀田倫史, 宮田健, 梅津光央

日本生物工学会大会講演要旨集　71st　2019
AIを利用したスマートホットライブラリーデザイン:AIはGFPをYFP化できるか?

及川未早来, 中澤光, 二井手哲平, 亀田倫史, 齋藤裕, 津田宏治, 津田宏治, 梅津光央, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　18th　2018
抗体移植を意識したβ-ヘアピン構造足場を利用した分子標的ペプチド取得

菊地真裕, 二井手哲平, 中澤光, 今中洋行, 梅津光央

日本生物工学会大会講演要旨集　70th　2018
AIはタンパク質進化を導くか?:機械学習支援によるGFPのYFP化検証

及川未早来, 齋藤裕, 亀田倫史, 中澤光, 二井手哲平, 津田宏治, 津田宏治, 梅津光央, 梅津光央

日本生物工学会大会講演要旨集　70th　2018
データベースからの小規模繰り返しライブラリー設計による抗体断片の発現向上

服部修平, 本田亜由美, 二井手哲平, 中澤光, 梅津光央

化学工学会秋季大会研究発表講演要旨集(CD-ROM)　50th　2018
終止コドンを除去したライブラリーを使った生体外選択法

伊藤智之, 西羽美, 亀田倫史, 二井手哲平, 中澤光, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　18th　2018
β-ヘアピン構造に着目したペプチド医薬と抗体医薬の同時開発

菊地真裕, 二井手哲平, 中澤光, 今中洋行, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　18th　2018
ドライとウェットの両側面からの抗体に代わる分子標的足場タンパク質の検討

伊藤智之, 西羽美, 亀田倫史, 二井手哲平, 中澤光, 梅津光央

日本生物工学会大会講演要旨集　70th　2018
低分子医薬候補分子の親和性向上を目指した薬剤修飾ペプチド提示ファージライブラリーの可能性

高橋拓人, 二井手哲平, 中澤光, 今中洋行, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　18th　2018
光に応答して活性化するハイブリッド酵素デザイン

中澤光, 石垣友理, 二井手哲平, 梅津光央

酵素工学研究会講演会講演要旨集　80th　2018
ファージ提示法とドメインライブラリー発想を連結した高活性T-cell recruiting抗体の迅速創出

杉山在生人, 中澤光, 村上明一, 岸本英博, 梅津光央

日本分子生物学会年会プログラム・要旨集(Web)　41st　2018
進化工学的操作と高速傷害活性評価システムを組み合わせた高活性T-cell recruiting抗体の迅速創出

杉山在生人, 中澤光, 村上明一, 岸本英博, 梅津光央

化学工学会秋季大会研究発表講演要旨集(CD-ROM)　50th　2018
親和性ペプチド分子による有機結晶の構造制御と生体分子-半導体有機結晶ハイブリッド材料作製プロセスの開発 (特集創造的プロセス開発の新展開)

二井手哲平, 中澤光, 梅津光央

ケミカルエンジニヤリング = Chemical engineering　62　(7)　483-487　2017/07
Publisher: 化学工業社
ISSN： 0387-1037
繰り返し飽和変異法の確実性を高める迅速な変異体群の作製と機能評価プロセスの開発

及川未早来, 中澤光, 二井手哲平, 亀田倫史, 齋藤裕, 津田宏治, 津田宏治, 梅津光央, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　17th　2017
段階的変異導入による効率的な指向的進化工学手法の構築

菊池沙也香, 服部峰充, 中澤光, 二井手哲平, 梅津光央

日本生化学会大会(Web)　90th　2017
低分子医薬の欠点を補完する有機低分子修飾ペプチドフォーマットの可能性

高橋拓人, 二井手哲平, 中澤光, 今中洋行, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　17th　2017
配列相同性を利用した難発現低分子抗体の発現量向上

服部修平, 熊谷維子, 中澤光, 二井手哲平, 金子美華, 加藤幸成, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　17th　2017
抗体データベースを利用した大規模変異導入による難発現抗体断片の発現量向上プロセスの提案

服部修平, 二井手哲平, 中澤光, 本田亜由美, 梅津光央

日本生物工学会大会講演要旨集　69th　2017
核酸医薬のDDSに利用可能な低分子抗体の設計

三浦大輔, 服部峰充, 二井手哲平, 中澤光, 梅津光央

日本生化学会大会(Web)　90th　2017
飽和変異法とライブラリー的手法の融合による効率的な指向的進化工学の提案

菊池沙也香, 服部峰充, 中澤光, 二井手哲平, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　17th　2017
抗体断片の発現量向上を目的とした抗体データベースによる大規模変異導入プロセスの提案

服部修平, 本田亜由美, 二井手哲平, 中澤光, 梅津光央

化学工学会秋季大会研究発表講演要旨集(CD-ROM)　49th　2017
タンパク質の機能改変を効率化するライブラリーデザインサイクルの提案

菊池沙也香, 服部峰充, 中澤光, 二井手哲平, 梅津光央

日本生物工学会大会講演要旨集　69th　2017
終止コドンを排除したライブラリー設計によるループペプチド開発

菊地真裕, 二井手哲平, 中澤光, 今中洋行, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　17th　2017
Contact orderから見た組換えタンパク質の物性相関

伊藤智之, 西羽美, 亀田倫史, 二井手哲平, 中澤光, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　17th　2017
二重特異性材料認識抗体によるナノ材料の集積化

二井手哲平, 真鍋法義, 中澤光, 熊谷泉, 梅津光央

日本蛋白質科学会年会プログラム・要旨集　17th　2017
Combination of molecular evolution and conformational information for antibody mimic

梅津光央, 中澤光, 二井手哲平

Medchem News (Web)　27　(1)　2017

ISSN： 2432-8626
低分子抗体-薬物複合体開発:リジン残基側鎖の化学反応へ界面活性剤が及ぼす影響

服部修平, 服部峰充, 中澤光, 二井手哲平, 松永淳, LEE Seon Hwa, 大江知行, 梅津光央

日本生物工学会大会講演要旨集　68th　2016
ファージ提示法を利用したCDR模倣ペプチドライブラリーの構築

柳沢美貴, 二井手哲平, 服部峰充, 中澤光, 熊谷泉, 梅津光央

日本生物工学会大会講演要旨集　68th　2016
難発現抗体断片を大腸菌発現させるデータベース利用

服部修平, 齋琢磨, 服部峰充, 中澤光, 二井手哲平, 亀田倫史, 熊谷維子, 金子美華, 加藤幸成, 梅津光央

日本分子生物学会年会プログラム・要旨集(Web)　39th　2016
蛋白質の分子認識を利用したプラズモニック膜と多孔質膜のボトムアップ作製プロセス

二井手哲平, 真鍋法義, 中澤光, 服部峰充, 熊谷泉, 梅津光央

化学工学会大会講演要旨集(CD-ROM)　2015　2015
1P-283 Biomass-binding peptides designed by molecular evolution for efficient degradation of cellulose in biomass by cellulase

Nakazawa Hikaru, Ishigaki Yuri, Ikeuchi Akinori, Kouda Katsunori, Yasutani Noriko, Onishi Toru, Umetsu Mitsuo

67　159-159　2015
Publisher: 日本生物工学会
2P-055 Domain library approach describes rules for highly cytotoxic antibody

Sugiyama Aruto, Umetsu Mitsuo, Nakazawa Hikaru, Asano Ryutaro, Kumagai Izumi

67　188-188　2015
Publisher: 日本生物工学会
2P-051 Development of an artificial cellulase utilizing cellulose-binding DNA aptamer

Takahara Mari, Mori Yutaro, Budinova Geisa A.L.G., Nakazawa Hikaru, Umetsu Mitsuo, Kamiya Noriho

67　187-187　2015
Publisher: 日本生物工学会
2P-056 Influence of amino acid sequence homology on dimerization of diabody-type

Onodera Tomoko, Umetsu Mitsuo, Tanaka Yoshikazu, Nakazawa Hikaru, Hattori takamitsu, Asano Ryutaro, Kumagai Izumi

67　188-188　2015
Publisher: 日本生物工学会
3P-036 How effective new small bispecific antibodies are created from existing antibody library

Sugiyama Aruto, Nakazawa Hikaru, Hosokawa Katsuhiro, Asano Ryutaro, Kumagai Izumi, Umetsu Mitsuo

66　203-203　2014
Publisher: 日本生物工学会
2P-057 Synergistic effect on catalytic activity of cellulase with a number of hybrid nano-cellulosomes designed from library-selected cellulose binding domains

Nakazawa Hikaru, Okada Izumi, Ishigaki Yuri, Kobayashi Eiko, Umetsu Mitsuo

66　121-121　2014
Publisher: 日本生物工学会
2P-058 Kimera cellulase design from module library approach

Okada Izumi, Nakazawa Hikaru, Ishigaki Yuri, Kobayashi Eiko, Umetsu Mitsuo

66　121-121　2014
Publisher: 日本生物工学会
2P-083 High performance artificial cellulosome design based on protein module library on nanomaterial

Nakazawa Hikaru, Ishigaki Yuri, Kumagai Izumi, Umetsu Mitsuo

65　124-124　2013
Publisher: 日本生物工学会
酸化亜鉛コーティングプラズモニックチップ上のサンドイッチアッセイによるうつ病関連マーカーBDNFの迅速検出

佐藤茉莉, 佐藤茉莉, 田和圭子, 上垣浩一, 原とも子, 梅津光央, 中澤光, 熊谷泉, 板倉誠, 板倉誠, 高橋正身, 高橋正身, 青田浩幸, 小島正己, 小島正己

高分子学会予稿集(CD-ROM)　62　(1)　2013
Ca1-2 セルロース系バイオマス分解におけるTrichoderma reeseiヘミセルラーゼの役割(セルラーゼおよび関連酵素,一般講演,日本応用糖質科学会平成24年度大会(第61回))

齋藤勇司, 長野まどか, 三井勇輔, 小松光子, 中澤光, 小笠原渉, 森川康, 二階堂満, 戸谷一英, 岡田宏文

応用糖質科学 : 日本応用糖質科学会誌　2　(3)　B39　2012/08/20
Publisher: 日本応用糖質科学会
ISSN： 2185-6427
Ca1-1 Trichoderma reesei CBH IIの親水性がバイオマス糖化に及ぼす影響(セルラーゼおよび関連酵素,一般講演,日本応用糖質科学会平成24年度大会(第61回))

小松光子, 齋藤勇司, 中澤光, 二階堂満, 戸谷一英, 小笠原渉, 森川康, 岡田宏文

応用糖質科学 : 日本応用糖質科学会誌　2　(3)　B38　2012/08/20
Publisher: 日本応用糖質科学会
ISSN： 2185-6427
4Ca15 Hybrid nanocellulosome: Nanocluster design of high performancve cellulases with multivalent cellulose-binding modules

Nakazawa Hikaru, Kim Do-myoung, Matsuyama Takashi, Ishida Nobuhiro, Ikeuchi Akinori, Kumagai Izumi, Umetsu Mitsuo

64　195-195　2012
Publisher: 日本生物工学会
うつ病候補マーカーであるBDNFの酸化亜鉛コーティングプラズモニックチップを用いた迅速・高感度検出

佐藤茉莉, 田和圭子, 上垣浩一, 原とも子, 梅津光央, 中澤光, 熊谷泉, 青田浩幸, 松本昭, 小島正己

日本化学会講演予稿集　92nd　(1)　2012

ISSN： 0285-7626
4Ia09 Role of endoglucanase I and II from Trichoderma reesei in biomass degradation

Mitsui Yusuke, Saito Yuji, Nagano Madoka, Komatsu Mitsuko, Nakazawa Hikaru, Ogasawara Wataru, Morikawa Yasushi, Okada Hirofumi, Totani Kazuhide, Nikaido Mitsuru

64　241-241　2012
Publisher: 日本生物工学会
3P-1050 Characterization of two endoglucanases from Lumbricus rubellus and saccharification of biomass

AKAZAWA Shin-ichi, IKARASHI Yuki, YARIMIZU Jun, YOKOYAMA Daichi, NAKAZAWA Hikaru, MORIKAWA Yasushi, OGASAWARA Wataru

22　70-70　2010
Publisher: 日本生物工学会
2Da09 Analysis of polysaccharide-degrading enzymes from Lumbricus rubellus

AKAZAWA Shin-ichi, YARIMIZU Jun, NAKAZAWA Hikaru, OGASAWARA Wataru, MORIKAWA Yasushi

21　45-45　2009
Publisher: 日本生物工学会
3Ga10 Disaccharide-condensing activity of Trichoderma reesei EG I catalytic domain

TOTANI Kazuhide, YOSHIDA Naoki, NAKAZAWA Hikaru, OGASAWARA Wataru, OKADA Hirofumi, MORIKAWA Yasushi

20　187-187　2008
Publisher: 日本生物工学会

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Books and Other Publications 6

微生物を活用した有用物質の製造技術

中澤光、梅津光央

シーエムシー出版　2023/05

ISBN: 9784781317403
生物工学会誌バイオミディア酵素はメカ化できるのか？

中澤光

生物工学会　2019/04
MEDCHEM NEWS

Mitsuo Umetsu, Hikaru Nakazawa, Teppei Niide

2017/02/01
ケミカルエンジニヤリング親和性ペプチド分子による有機結晶の構造制御と生体分子-半導体有機結晶ハイブリッド材料作製プロセスの開発

二井手哲平, 中澤光, 梅津光央

化学工業社　2017/01
進化分子工学

梅津光央, 中澤光, 服部峰充

エヌティー・エス　2013
バイオマス分解関連酵素研究の最前線

梅津光央, 金渡明, 中澤光

シーエムシー出版　2012

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Presentations 7

Machine learning-assisted molecular evolution for stable enzyme engineering Invited

Hikaru Nakazawa

25th NEA symposium Synthetic Biology 2024　2024/09/10
タンパク質の自動設計は可能になるのか？：ドメインライブラリアプローチと機械学習支援 Invited

中澤光

日本化学会バイオ関連化学若手フォーラム　2019/09/03
蛋白質機能を向上するためのドメインライブラリー設計 Invited

中澤光

日本化学会東北支部会　2019/07/12
分子認識アプタマー開発における進化分子工学操作の新しい展開 Invited

中澤光, 梅津光央

日本分析化学会第79回分析化学討論会マイクロ・ナノで生命を測る　2019/05/14
共役効果を意識したモジュールライブラリー発想人工セルロソーム設計 Invited

中澤光, 石垣友理, 岡田和, 小林栄子, 梅津光央

第29回セルラーゼ研究会　2015/07/17
モジュールライブラリーという発想からの進化工学的人工セルロソーム設計 Invited

中澤光, 石垣友理, 岡田和, 小林栄子, 梅津光央

第28回セルラーゼ研究会　2014/07/11
酵素共役系を意識したモジュール集積によるハイブリッドセルロソームの設計 Invited

中澤光, 金渡明, 熊谷泉, 梅津光央

新学術領域融合マテリアル：分子制御による材料創成と機能開拓, 第五回若手スクール　2012/11/26

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Industrial Property Rights 7

糖化残差成分結合能を有するペプチドおよびその利用

Property Type: Patent
セルロース系バイオマスからの糖およびアルコールの製造方法

5745237号

Property Type: Patent
ダイアボディ型BsAbを発現・分泌するビフィドバクテリウム属細菌

嶋谷裕子, 小林聡, 塩谷公一郎, 片岡之郎, 関雄史, 松村富穂, 金成安慶, 梅津光央, 中澤光

Property Type: Patent
プラスミド一周ＰＣＲ法を用いたランダム遺伝子プラスミドライブラリー作成方法、それを用いた形質転換体作成方法、および、その方法に使用するキット

中澤光, 柳沢美貴, 二井手哲平, 服部峰充, 梅津光央

Property Type: Patent
抗体断片及び該抗体を含む二重特異性抗体

梅津光央, 杉山在生人, 中澤光, 二井手哲平, 岸本英博, 村上明一

Property Type: Patent
標的分子と結合するポリペプチド

梅津光央, 伊藤智之, 西羽美, 中澤光, 二井手哲平, 亀田倫史

Property Type: Patent
セルロース系バイオマスからの糖及びアルコールの製造方法、並びに該方法に用いられる微生物

中澤光, 小笠原渉, 岡田宏文, 森川康, 小林良則, 川口剛司, 炭谷順一, 谷修治

Property Type: Patent

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Research Projects 7

機械学習×進化工学が指し示すタンパク質のif進化：創薬酵素の高度成熟・機能化

梅津光央, 中澤光, 伊藤智之, 田中良和

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2024/04/01 - 2027/03/31
精密移植＆フィッティング技術により挑戦する金属表面滑走モータータンパク質創出

中澤光

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的研究(萌芽)

Institution: 東北大学

2022/06/30 - 2025/03/31

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キネシンはモータータンパク質として知られており、「走る」という動的性質からナノスケールのアクチュエーターとして期待されており、最近ではアメーバ状ナノロボットや繊維状ナノロボットの駆動力として使われている。キネシンのマイクロチューブリン上しか移動できないという使用の制限を解決するために、本申請は、マイクロチューブリン表面以外の無機材料表面を走るキネシンタンパク質の開発に挑戦した。キネシンは、ループL11とループL12によって微小管の表面に繰り返し存在するそれぞれの相互作用部分とATP依存で交互に結合することによって、走っていることがわかってきた。結合は物理吸着であることから、申請者は「走る」ためには、キネシンの動く相互作用部位と、結合対象の「8nm」の幅で繰り返す相互作用部位が重要と考えた。本申請では①キネシンの相互作用ループへの結合ペプチドの移植および②相互作用部位を一定の間隔で配置したナノマテリアル構造体の作製を行い、無機材料表面を走るキネシンタンパク質の開発にチャレンジする。当該年度はC末端ビオチン化したNeurospora Crassa 由来のキネシンをすでに大腸菌を宿主として大量発現させていたが、調製に難航した。大腸菌にてより発現量の多いキネシンが見つかったためそれを用いた方が今後の実験が円滑に進むと考え、新たに、タグ融合遺伝子構築を行った。このキネシンを用いて所属する研究室でペプチド移植を進めていくこととする。移植に関してはまだ行っていないが、予定通り金結合ペプチドおよび酸化亜鉛結合ペプチド）の長さを調節し、キネシンのループL11とループL12それぞれに移植、あるいはシリカ結合ペプチドを両ループに移植することを想定して遺伝子を設計した。
光－動力変換ナノデバイスの動作原理に関する予備検討

Offer Organization: 国立研究開発法人科学技術振興機構（JST）

System: 戦略的創造研究推進事業（さきがけ「光融合」）特定課題調査

Category: 戦略的創造研究推進事業（さきがけ「光融合」）特定課題調査

Institution: 東北大学

2024/10 - 2025/03
光照射で酵素の活性を向上するスキャフォ―ルド粉体の開発

Offer Organization: ホソカワ粉体工学振興財団

System: 研究助成

Category: 研究助成

Institution: 東北大学

2021/04 - 2022/03
Nanobio antifungal agent for fungal pandemics

NAKAZAWA HIKARU

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2018/04/01 - 2021/03/31

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Japanese mold (Candida aureas) has become poisonous overseas, causing the first mycotic pandemic (global epidemic) in Europe, the United States and Asia.The development of antifungal agents with a new mechanism of action that is difficult to have resistance is expected.In this study, we have developed a nanobio antifungal agent that exhibits antifungal activity by using the natural antifungal enzyme chitinase on the surface of silver nanoparticles that have high antifungal activity and are less likely to develop resistance.
An artificial enzyme for improvement of enzymatic activity by irradiation of sun light.

Nakazawa Hikaru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

Category: Grant-in-Aid for Young Scientists (B)

Institution: Tohoku University

2016/04/01 - 2019/03/31

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Non-edible biomass expected and its convert to energy and chemicals. In case of use of rigid cellulose. It is necessary to degrade for long period. Enzymatic activity should be enhanced naturally without heat source like boiler. Here, we constructed novel enzyme design for eco-carbon cycles by use of star shape gold nanoparticles with conversion of sun light to heat. Consequently, clustering effect of cellulase module on the surface of nanoparticle and enhanced reaction activity in nanoparticle surface by Light-heat effect of Gold nanostar result in 5 times increase in saccharification activity of cellulase module.
Application of hybrid nano-assembly for in-vitro synthetic biology tool

Umetsu Mitsuo

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2016/04 - 2019/03

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In vivo, a series of enzymes are closely packed in a minute space, and the enzyme reactions are coupled. In this study, enzymes are clustered on nanoparticles and the clustered enzymes are hierarchically assembled to reconstruct in-vivo-mimic enzyme-crowded environment. The utilization of material-binding peptides and proteins showed the possibility for spontaneous self-assembly of clustered enzymes.

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Teaching Experience 5

Molecular Biological Engineering　Tohoku University
Chemical and Biomolecular Engineering II　Tohoku University
Biofunctional Chemistry　Tohoku University
Life Sciences B　Tohoku University
Exercises B　Tohoku University

Social Activities 1

タンパク質の進化分子工学×AIでバイオ医薬品の開発

地域創造学

2024/11/05 - Present