Details of the Researcher

PHOTO

Yuji Tanaka
Section
New Industry Creation Hatchery Center
Job title
Specially Appointed Associate Professor(Research)
Degree

Research History 14

  • 2026/04 - Present
    Tohoku University　New Industry Creation Hatchery Center, Age-Adaptive Project for Centenarian Mobility

  • 2025/10 - Present
    Tohoku University Hospital　Endowed Research on Division Geriatric Sleep Medicine and Social Safety Development

  • 2024/04 - Present
    東北大学大学院農学研究科

  • 2024/02 - Present
    Tohoku University　New Industry Creation Hatchery Center

  • 2018 - Present
    ナノ医療イノベーションセンター　客員研究員

  • 2023/04 - 2024/01
    Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology

  • 2019/10 - 2023/09
    公立大学法人横浜市立大学視覚再生外科学　客員研究員

  • 2022/04 - 2023/03
    国立大学法人 大阪大学　先導的学際研究機構共生知能システム研究センター　招へい研究員

  • 2018/07 - 2023/03
    University of Yamanashi　Graduate Faculty of Interdisciplinary Research Faculty of Medicine

  • 2018/07 - 2020/03
    理化学研究所　客員研究員

  • 2017 - 2018
    理化学研究所　多細胞システム研究センター　研究員

  • 2014 - 2017
    理化学研究所　情報基盤センター　センター研究員

  • 2011 - 2014
    東北大学大学院　医学系研究科　助教

  • 2010 - 2011
    東京女子医大　先端生命医科学研究所　博士研究員

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Education 4

  • 東北大学大学院　医学系研究科

    2006 - 2010

  • 東北大学大学院　工学研究科

    2004 - 2006

  • Tohoku University　Faculty of Engineering

    1999 - 2004

  • University of Waterloo

    2003 - 2003

Committee Memberships 2

  • 韮崎市長寿介護課　解析・評価員

    2020/04 - 2023/03

  • 富士河口湖町シニアドライバー支援事業委員会　委員

    2020/04 - 2023/03

Professional Memberships 3

  • 日本老年医学会

    2022 -

  • 臓器保存学会

  • THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE

Research Areas 1

  • Life sciences / Applied molecular and cellular biology /

Papers 50

  1. Modular Control of PEG-Poly(2-Oxazoline) Co-Assembly Enables Tunable Aggregate Properties for Intraocular Pressure Management. International-journal Peer-reviewed

    Yuji Tanaka, Shohei Morikawa, Shigehito Osawa

    Advanced healthcare materials　e04206　2026/04/09

    DOI： 10.1002/adhm.202504206 　

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    Poly(2-oxazoline) (POx) polymers are hydrophilic and biocompatible materials with tunable physicochemical properties for biomedical use. Postoperative hypotony, which currently lacks approved pharmacological or device-based treatment, requires minimally invasive and controllable methods for temporary intraocular pressure restoration. Herein, we present a modular strategy to control POx aggregation via thermally triggered co-assembly with thermoswitchable poly(2-n-propyl-2-oxazoline) (PnPrOx) and hydrophilic PEG-modified PnPrOx (PnPrOx-PEG). These mixtures remain dissolved at low temperatures but aggregate at physiological temperatures because of the thermo-switchable behavior of the PnPrOx segments, with PEG content modulating particle size, dispersibility, and fusion. Based on in vivo compositional screening in rabbits, we highlight a 98:2 (w/w) PnPrOx:PnPrOx-PEG formulation (POx2P) that induces transient intraocular pressure elevation without persistent turbidity or deposition. In a trabeculectomy-induced hypotony model, POx2P restored intraocular pressure without adverse effects. Microscopy and in vivo imaging suggest that this formulation forms flow-responsive aggregates that transiently occlude aqueous outflow pathways. We established a compositionally tunable and injectable POx platform for in situ aggregation and pressure modulation, offering a clinically relevant approach for localised intraocular therapy.

  2. Minimum pulse rate during sleep as a MMSE-associated factor: a multifaceted exploratory study in older adults without long-term care certification. International-journal Peer-reviewed

    Yuji Tanaka, Kozo Saito, Kyoichiro Tsuchiya, Yusuke Iwata, Takashi Ando, Kazuki Mochizuki, Tamami Taniguchi, Takahiko Mitsui

    BMC geriatrics　26　(1)　106-106　2025/12/20

    DOI： 10.1186/s12877-025-06584-3 　

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    BACKGROUND: Developing quantitative indicators of daily life that detect subtle cognitive decline is an important challenge in today's growing population of older adults worldwide. In this study, we aimed to explore novel candidates for indicators associated with subtle abnormalities in brief dementia screening test. METHODS: In this multifaceted cross-sectional study, data were collected from 35 older adults who had not been certified for long-term care or diagnosed with cognitive impairment using questionnaires, the Mini-Mental State Examination (MMSE), body composition measurements, sleep apnoea testing, activity monitoring, motor function assessments, blood tests, and nutrient analyses. Of the 89 factors examined in this study, several less invasive indicators for cognitive impairment were identified using Spearman's correlation analysis, two-group comparison, and multiple linear regression model analysis. RESULTS: An increased minimum pulse rate during sleep was found to be the most important non-invasive marker associated with both the correlation with MMSE scores and the risk classification of cognitive impairment (mild cognitive impairment or dementia). CONCLUSIONS: The minimum pulse rate during sleep is easily detectable owing to recent developments and widespread use of wearable devices. The results of this study will not only provide opportunities for older adults and their families to recognise slight cognitive decline but will also contribute to the early detection of cognitive decline, as a guide for the development of research evaluating the detection sensitivity and specificity of these indicators.

  3. Effects of feeding the medium-chain triacylglycerol on GLP-1 secretion in aged Brd4-heterozygous mice, which are a mouse model of aging Peer-reviewed

    Musashi Kawamura, Natsuyo Hariya, Shiori Ishiyama, Yuji Tanaka, Keiko Ozato, Kazuki Mochizuki

    Nutrition　131　112656-112656　2025/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.nut.2024.112656 　

    ISSN： 0899-9007

  4. Effect of the Sugar Present in the Culture Medium on the Preservation of Human RPE Cell Suspensions. International-journal Peer-reviewed

    Shohei Kitahata, Hinako Ichikawa, Yuji Tanaka, Shin Tanaka, Tatsuya Inoue, Maiko Maruyama-Inoue, Kazuaki Kadonosono

    Translational vision science & technology　14　(2)　1-1　2025/02/03

    DOI： 10.1167/tvst.14.2.1 　

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    PURPOSE: The retinal pigment epithelium (RPE) is crucial for photoreceptor function, and its dysfunction is associated with several retinal degenerative diseases. This study examines how different sugars in preservation media affect the viability of RPE cells, highlighting the need for effective storage solutions for cell transplantation. METHODS: Human RPE cells were cultured and suspended in modified media with various sugars. The survival rate was measured for cells cultured under adhesion for 3 weeks and for those stored at 37°C for 24 hours. Metabolism was evaluated using liquid chromatography, whereas the responses to hypoxia were assessed with specific markers. RESULTS: Fructose-containing media significantly enhanced RPE cell survival, even under hypoxic conditions. In adherent cultures, fructose showed higher survival rates compared to galactose, which had a notably low survival rate. Chromatography results indicated that fructose played a role in non-anaerobic metabolism, helping to explain its effectiveness. In suspension, fructose maintained higher cell viability than glucose and suppressed hypoxia markers, suggesting increased resistance to hypoxic stress. CONCLUSIONS: The study highlights the critical role of sugar composition in preservation media on RPE cell survival, positioning fructose as a potential enhancer. Its antioxidative properties suggest fructose could be effective in suspension preservation. These findings indicate that fructose-containing media are promising for preserving RPE cells and could have broader applications in preserving various cell types and tissues. TRANSLATIONAL RELEVANCE: The results of this study may allow for longer-term storage of RPE cells, potentially increasing the versatility of cell transplantation therapy.

  5. Pigmentation level of human iPSC-derived RPE does not indicate a specific gene expression profile. International-journal Peer-reviewed

    Yoko Nakai-Futatsugi, Jianshi Jin, Taisaku Ogawa, Noriko Sakai, Akiko Maeda, Ken-Ichi Hironaka, Masakazu Fukuda, Hiroki Danno, Yuji Tanaka, Seiji Hori, Katsuyuki Shiroguchi, Masayo Takahashi

    eLife　12　2024/05/09

    DOI： 10.7554/eLife.92510 　

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    Retinal pigment epithelium (RPE) cells show heterogeneous levels of pigmentation when cultured in vitro. To know whether their color in appearance is correlated with the function of the RPE, we analyzed the color intensities of human-induced pluripotent stem cell-derived RPE cells (iPSC-RPE) together with the gene expression profile at the single-cell level. For this purpose, we utilized our recent invention, Automated Live imaging and cell Picking System (ALPS), which enabled photographing each cell before RNA-sequencing analysis to profile the gene expression of each cell. While our iPSC-RPE were categorized into four clusters by gene expression, the color intensity of iPSC-RPE did not project any specific gene expression profiles. We reasoned this by less correlation between the actual color and the gene expressions that directly define the level of pigmentation, from which we hypothesized the color of RPE cells may be a temporal condition not strongly indicating the functional characteristics of the RPE.

  6. Height and Weight, Not Body Mass Index, Are Closely Associated With Activities of Daily Living in Japanese Older Adults. International-journal Peer-reviewed

    Yuji Tanaka, Takashi Ando, Kyoichiro Tsuchiya, Kazuki Mochizuki

    Asia-Pacific journal of public health　10105395241247336-10105395241247336　2024/04/20

    DOI： 10.1177/10105395241247336 　

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    Body mass index (BMI) is routinely used to ascertain health status, including activities of daily living (ADLs); however, the associations of ADLs with height and weight in older adults have not been elucidated. Therefore, we cross-sectionally investigated the correlations between ADLs and height, weight, and BMI in 155 participants aged 82 to 103 years and characterized the naïve Bayesian prediction for ADLs. Activities of daily living showed a significant negative correlation with height and weight and a positive correlation with age. In males, a shorter height was associated with an increased risk of falling and disability in phone calling independently, and losing weight was associated with an increased risk of disability in going out. Combining age, weight, and height improved the area under the receiver operating characteristic curve in the prediction of disability in going out and phone calling independently in males. Therefore, height and weight, not BMI, are potential predictors of ADL decline.

  7. Investigation of the effectiveness of gelatin hydrolysate in human iPS-RPE cell suspension transplantation. International-journal Peer-reviewed

    Shohei Kitahata, Michiko Mandai, Hinako Ichikawa, Yuji Tanaka, Toshika Senba, Keisuke Kajita, Sunao Sugita, Kazuaki Kadonosono, Masayo Takahashi

    Regenerative therapy　25　238-249　2024/03

    DOI： 10.1016/j.reth.2023.12.016 　

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    INTRODUCTION: The retinal pigment epithelium (RPE) plays essential roles in maintaining retinal functions as well as choroidal capillaries and can lead to visual disorders if dysfunctional. Transplantation of human-induced pluripotent stem cell-derived RPE (hiPSC-RPE) is a promising therapy for such RPE impaired conditions including age-related macular degeneration. The challenge with cell suspension transplantation is targeted delivery of graft cells and undesired cell reflux. Gelatin hydrolysate, a soluble variant with specific molecular weight distribution, is examined in this study for its potential use in hiPSC-RPE suspension transplantation, particularly in reducing cell reflux and enhancing RPE engraftment. METHODS: A retinal bleb model was created using polydimethylsiloxane (PDMS) soft lithography to quantify cellular reflux. We examined the effects of gelatin hydrolysate on the hiPSC-RPE of various aspects of cell behavior and performance such as cell viability, hypoxia reaction, morphology, induction of inflammation and immune responses. RESULTS: Gelatin hydrolysate at 5 % concentration effectively mitigated cell reflux in vitro mimic, improved cell viability, reduced cell aggregation, and had an inhibitory effect on hypoxic reactions due to cell deposition with hiPSC-RPE. Additionally, gelatin hydrolysate did not affect cell adhesion and morphology, and decreased the expression of major histocompatibility complex class II molecules, which suggests reduced immunogenicity of hiPSC-RPE. CONCLUSION: Gelatin hydrolysate is considered a valuable and useful candidate for future regenerative therapies in hiPSC-RPE suspension transplantation.

  8. Sleep status of older adults with sleep apnoea syndrome may vary by body mass index. International-journal Peer-reviewed

    Yuji Tanaka, Naana Baba-Mori, Takaaki Yonaga, Kazuki Mochizuki, Satoshi Igarashi, Takashi Ando, Takashi Kohda, Yasumi Ito, Kenzo Soejima, Daiju Sakurai

    Frontiers in aging　5　1331448-1331448　2024

    DOI： 10.3389/fragi.2024.1331448 　

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    Obesity and ageing are the most important risk factors for sleep apnoea syndrome (SAS); however, the role of body mass index (BMI) on sleep status in healthy older adults is unclear. To explore sleep parameters according to BMI among active older adults, we cross-sectionally examined the relationship between sleep-related parameters and BMI in 32 Japanese adults aged from 83 to 95 years without long-term care who were unaware of having SAS. Correlation and linear regression analyses were performed. Moderate or severe SAS prevalence was high in both those with low (68.8%) and high (68.8%) BMI. A higher increase in apnoea-hypopnoea index (AHI) was negatively correlated with sleep depth in the high-BMI group. In the low-BMI group, the number of awakenings and age were positively correlated with AHI. Older adults may have SAS regardless of their BMI, and the sleep status of patients with SAS may vary by BMI.

  9. Dietary medium-chain triglycerides upregulate metabolic gene expression in the gastrocnemius and downregulate expression of muscle-degradation gene in the soleus in aged bromodomain-containing 4 heterozygous mice Peer-reviewed

    Mayu Kimura, Shiori Ishiyama, Takashi Ando, Yuji Tanaka, Keiko Ozato, Kazuki Mochizuki

    PharmaNutrition　26　100361-100361　2023/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.phanu.2023.100361 　

    ISSN： 2213-4344

  10. Super-multifactorial survey YHAB revealed high prevalence of sleep apnoea syndrome in unaware older adults and potential combinatorial factors for its initial screening Peer-reviewed

    Yuji Tanaka, Takashi Ando, Kazuki Mochizuki, Satoshi Igarashi, Kyoichiro Tsuchiya, Kozo Saito, Yasumi Ito, Zentaro Yamagata, Masaru Iwasaki, YHAB Health Data Survey Group 2020

    Frontiers in Aging　3　2022/10/14

    Publisher: Frontiers Media SA

    DOI： 10.3389/fragi.2022.965199 　

    eISSN： 2673-6217

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    Study Objectives: Aging is a risk factor for sleep apnoea syndrome (SAS), which is associated with lower quality of life and sudden mortality. However, SAS is often overlooked in older adults without suspicions. Therefore, this study aimed to evaluate SAS incidence and 48 other general factors in older adults. Methods: This cross-sectional study included all non-caregiver-certified, healthy individuals (N = 32) who survived during the long-term cohort study and agreed to participate in apnoea-hypopnoea index (AHI) measurement (aged 83–95 years). AHI and 48 other general factors were evaluated, and simple linear regression analysis was used to identify potential AHI-related factors. Stepwise evaluation was further performed using multiple linear regression analyses. Results: Although no individuals were previously diagnosed with SAS, 30 (93.75%) participants had some degree of SAS (AHI > 5/h), and 22 (68.75%) had severe or moderate SAS (AHI > 15/h). Compared with typical single risk factors represented by body mass index, combining daily steps and other factors improved the fit to the multiple linear regression. Combining daily steps and body mass index improved the fit for males and combining daily steps and red blood cell count improved the fit for females. Conclusion: SAS was highly prevalent in unaware healthy Japanese older adults; combinations of daily steps and body mass index, and daily steps and red blood cell count may predict AHI in such individuals without the need for a specific AHI test.

  11. Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion Peer-reviewed

    Mitsuhiro Nishida, Yuji Tanaka, Yo Tanaka, Satoshi Amaya, Nobuyuki Tanaka, Hirofumi Uyama, Tomohiro Masuda, Akishi Onishi, Junki Sho, Satoshi Yokota, Masayo Takahashi, Michiko Mandai

    Scientific Reports　11　(1)　2021/11/02

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-021-00703-x 　

    eISSN： 2045-2322

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    Abstract Several clinical studies have been conducted into the practicality and safety of regenerative therapy using hESC/iPSC-retinal pigment epithelium (RPE) as a treatment for the diseases including age-related macular degeneration. These studies used either suspensions of RPE cells or an RPE cell sheet. The cells can be injected using a minimally invasive procedure but the delivery of an intended number of cells at an exact target location is difficult; cell sheets take a longer time to prepare, and the surgical procedure is invasive but can be placed at the target area. In the research reported here, we combined the advantages of the two approaches by producing a quickly formed hiPSC-RPE strip in as short as 2 days. The strip readily expanded into a monolayer sheet on the plate, and after transplantation in nude rats, it showed a potency to partly expand with the correct apical/basal polarity in vivo, although limited in expansion area in the presence of healthy host RPE. The strip could be injected into a target area in animal eyes using a 24G canula tip.

  12. Transient rho-associated coiled-coil containing kinase (ROCK) inhibition on human retinal pigment epithelium results in persistent Rho/ROCK downregulation International-journal Peer-reviewed

    Shohei Kitahata, Hinako Ichikawa, Yuji Tanaka, Tatsuya Inoue, Kazuaki Kadonosono

    Biochemistry and Biophysics Reports　24　100841-100841　2020/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbrep.2020.100841 　

    ISSN： 2405-5808

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    Retinal pigment epithelium (RPE) cells is the outermost layer of the retina and RPE dysfunction is a key factor in the disease pathogenesis of age-related macular degeneration (AMD). Transplantation therapy using induced pluripotent stem cell (iPSC)-derived RPEs has recently received much attention as a treatment for AMD. Preserving these cells under the best possible conditions is important, and preservation methods using Y-27632 have been reported. Rho-associated coiled-coil containing kinase (ROCK) inhibitors are known to inhibit cell death, emerging as important drug candidates for stem cell differentiation and regenerative medicine. However, it has recently been shown that ROCK inhibitors may have a vasodilatory effect on human retinal arterioles, a side effect that should ideally be avoided in RPE transplantation. Although ROCK inhibitors hold great potential, optimizing efficacy while minimizing adverse reactions is critical for translation into a clinical treatment. We examined the effect of transient exposure of RPE cells to ROCK inhibitor Y-27632 to determine whether the extracellular presence of the drug is necessary for ongoing Rho/ROCK downregulation. Human RPE cells were subcultured as a suspension for 4 h in drug-free medium following exposure to Y-27632 for 2 h. A Y-27632 concentration of >10 μM improved cell survival beyond 4 h and cell proliferation in recovery culture medium. ROCK2 expression levels were specifically downregulated by Y-27632 in the Rho/ROCK signaling pathway. In conclusion, we demonstrated that the effect of Y-27632 is not dependent on its extracellular availability and can last beyond the 2 h of exposure. The lasting Rho/ROCK signaling pathway downregulation by Y-27632 suggests that RPE cell transplantation with ROCK inhibitor-free media is possible, which can minimize side effects to host tissue and have wider implications for transplantation methods requiring ROCK inhibition.

  13. A simple and static preservation system for shipping retinal pigment epithelium cell sheets. International-journal Peer-reviewed

    Hori K, Kuwabara J, Tanaka Y, Nishida M, Koide N, Takahashi M

    Journal of tissue engineering and regenerative medicine　13　(3)　459-468　2019/03

    DOI： 10.1002/term.2805 　

    ISSN： 1932-6254

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    The ability to move cells and tissues from bench to bedside is an essential aspect of regenerative medicine. In this study, we propose a simple and static shipping system to deliver tissue-engineered cell sheets. Notably, this system is electronic-device-free and simplified to minimize the number of packing and opening steps involved. Shipping conditions were optimized, and application and verification of the system were performed using human iPS cell-derived or fetal retinal pigment epithelium (RPE) cell sheets. The temperature of the compartments within the insulated container was stable at various conditions, and filling up the cell vessel with medium effectively prevented turbulence-induced mechanical damage to the RPE cell sheets. Furthermore, no abnormal changes were observed in RPE morphology, transepithelial electrical resistance, or mRNA expression after transit by train and car. Taken together, our simple shipping system has the potential to minimize the costs and human error associated with bench to bedside tissue transfer. This specially designed regenerative tissue shipping system, validated for use in this field, can be used without any special training. This study provides a procedure for easily sharing engineered tissues with the goal of promoting collaboration between laboratories and hospitals and enhancing patient care.

  14. Critical Functionality Effects from Storage Temperature on Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cell Suspensions. International-journal Peer-reviewed

    Shohei Kitahata, Yuji Tanaka, Kanji Hori, Cody Kime, Sunao Sugita, Hiroshi Ueda, Masayo Takahashi

    Scientific reports　9　(1)　2891-2891　2019/02/27

    DOI： 10.1038/s41598-018-38065-6 　

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    Human induced pluripotent stem cell (hiPSC)-derived retinal pigment epithelium (hiPSC-RPE) cells suspension have the potential for regenerative treatment. However, practical regenerative applications with hiPSC-RPE cells require the development of simple and cost-effective non-freezing preservation methods. We investigated the effect of non-freezing temperatures on suspended hiPSC-RPE cells in various conditions and analysed mechanisms of cell death, anoikis, Rho GTPases, hypoxia, microtubule destruction, and cell metabolism. Cells stored at 37 °C had the lowest viability due to hypoxia from high cell metabolism and cell deposits, and cells preserved at 4 °C were damaged via microtubule fragility. Cell suspensions at 16 °C were optimal with drastically reduced apoptosis and negligible necrosis. Moreover, surviving cells proliferated and secreted key proteins normally, compared to cells without preservation. hiPSC-RPE cell suspensions were optimally preserved at 16 °C. Temperatures above or below the optimal temperature decreased cell viability significantly yet differentially by mechanisms of cell death, cellular metabolism, microtubule destruction, and oxygen tension, all relevant to cell conditions. Surviving cells are expected to function as grafts where high cell death is often reported. This study provides new insight into various non-freezing temperature effects on hiPSC-RPE cells that are highly relevant to clinical applications and may improve cooperation between laboratories and hospitals.

  15. Recent Advances in Biosynthetic Corneal Substitutes Peer-reviewed

    Yokokura S, Tanaka Y

    Current Ophthalmology Reports　2018/07

  16. Promoter-level transcriptome in primary lesions of endometrial cancer identified biomarkers associated with lymph node metastasis Peer-reviewed

    Emiko Yoshida, Yasuhisa Terao, Noriko Hayashi, Kaoru Mogushi, Atsushi Arakawa, Yuji Tanaka, Yosuke Ito, Hiroko Ohmiya, Yoshihide Hayashizaki, Satoru Takeda, Masayoshi Itoh, Hideya Kawaji

    SCIENTIFIC REPORTS　7　(1)　14160　2017/10

    DOI： 10.1038/s41598-017-14418-5 　

    ISSN： 2045-2322

  17. Creation of nano eye-drops and effective drug delivery to the interior of the eye Peer-reviewed

    Yoshikazu Ikuta, Shigenobu Aoyagi, Yuji Tanaka, Kota Sato, Satoshi Inada, Yoshitaka Koseki, Tsunenobu Onodera, Hidetoshi Oikawa, Hitoshi Kasai

    SCIENTIFIC REPORTS　7　44229　2017/03

    DOI： 10.1038/srep44229 　

    ISSN： 2045-2322

  18. Transparent, resilient human amniotic membrane laminates for corneal transplantation Peer-reviewed

    Takehiro Hariya, Yuji Tanaka, Shunji Yokokura, Toru Nakazawa

    BIOMATERIALS　101　76-85　2016/09

    DOI： 10.1016/j.biomaterials.2016.05.038 　

    ISSN： 0142-9612

    eISSN： 1878-5905

  19. Transcriptome profiling of the rat retina after optic nerve transection Peer-reviewed

    Masayuki Yasuda, Yuji Tanaka, Kazuko Omodaka, Koji M. Nishiguchi, Orie Nakamura, Satoru Tsuda, Toru Nakazawa

    SCIENTIFIC REPORTS　6　28736　2016/06

    DOI： 10.1038/srep28736 　

    ISSN： 2045-2322

  20. Real-time imaging of RGC death with a cell-impermeable nucleic acid dyeing compound after optic nerve crush in a murine model Peer-reviewed

    Satoru Tsuda, Yuji Tanaka, Hiroshi Kunikata, Yu Yokoyama, Masayuki Yasuda, Azusa Ito, Toru Nakazawa

    EXPERIMENTAL EYE RESEARCH　146　179-188　2016/05

    DOI： 10.1016/j.exer.2016.03.017 　

    ISSN： 0014-4835

    eISSN： 1096-0007

  21. Transcriptome analysis identifies genes, enhancer RNAs and repetitive elements that are recurrently deregulated across multiple cancer types Peer-reviewed

    Kaczkowski B, Tanaka Y, Kawaji H, Sandelin A, Andersson R, Itoh M, Lassmann T, Hayashizaki Y, Carninci P, Forrest A. R. R, FANTOMS Consortium

    Human Genomics　10　2016

  22. Transcriptome Analysis of Recurrently Deregulated Genes across Multiple Cancers Identifies New Pan-Cancer Biomarkers Peer-reviewed

    Bogumil Kaczkowski, Yuji Tanaka, Hideya Kawaji, Albin Sandelin, Robin Andersson, Masayoshi Itoh, Timo Lassmann, Yoshihide Hayashizaki, Piero Carninci, Alistair R. R. Forrest

    CANCER RESEARCH　76　(2)　216-226　2016/01

    DOI： 10.1158/0008-5472.CAN-15-0484 　

    ISSN： 0008-5472

    eISSN： 1538-7445

  23. Relationship of Skin Autofluorescence to Severity of Retinopathy in Type 2 Diabetes Peer-reviewed

    Masayuki Yasuda, Masahiko Shimura, Hiroshi Kunikata, Hiroko Kanazawa, Kanako Yasuda, Yuji Tanaka, Hideyuki Konno, Mai Takahashi, Taiki Kokubun, Kazuichi Maruyama, Noriko Sato, Masako Kakizaki, Mari Sato, Ichiro Tsuji, Toshio Miyata, Toru Nakazawa

    CURRENT EYE RESEARCH　40　(3)　338-345　2015/03

    DOI： 10.3109/02713683.2014.918152 　

    ISSN： 0271-3683

    eISSN： 1460-2202

  24. β2-Microglobulin is an appropriate reference gene for RT-PCR-based gene expression analysis of hematopoietic stem cells Peer-reviewed

    Yu Matsuzaki, Terumasa Umemoto, Yuji Tanaka, Teruo Okano, Masayuki Yamato

    Regenerative Therapy　1　91-97　2015

    Publisher: Japanese Society of Regenerative Medicine

    DOI： 10.1016/j.reth.2015.04.003 　

    ISSN： 2352-3204

  25. Artemin Augments Survival and Axon Regeneration in Axotomized Retinal Ganglion Cells Peer-reviewed

    Kazuko Omodaka, Takuji Kurimoto, Orie Nakamura, Kota Sato, Masayuki Yasuda, Yuji Tanaka, Noriko Himori, Yu Yokoyama, Toru Nakazawa

    JOURNAL OF NEUROSCIENCE RESEARCH　92　(12)　1637-1646　2014/12

    DOI： 10.1002/jnr.23449 　

    ISSN： 0360-4012

    eISSN： 1097-4547

  26. Retinal transcriptome profiling at transcription start sites: a cap analysis of gene expression early after axonal injury Peer-reviewed

    Masayuki Yasuda, Yuji Tanaka, Koji M. Nishiguchi, Morin Ryu, Satoru Tsuda, Kazuichi Maruyama, Toru Nakazawa

    BMC GENOMICS　15　982　2014/11

    DOI： 10.1186/1471-2164-15-982 　

    ISSN： 1471-2164

  27. Experimental Application of Piezoelectric Actuator-Driven Pulsed Water Jets in Retinal Vascular Surgery Peer-reviewed

    Hiroshi Kunikata, Yuji Tanaka, Naoko Aizawa, Atsuhiro Nakagawa, Teiji Tominaga, Toru Nakazawa

    TRANSLATIONAL VISION SCIENCE & TECHNOLOGY　3　(6)　10　2014/11

    DOI： 10.1167/tvst.3.6.10 　

    ISSN： 2164-2591

  28. Neuroprotective effect against axonal damage-induced retinal ganglion cell death in apolipoprotein E-deficient mice through the suppression of kainate receptor signaling Peer-reviewed

    Kazuko Omodaka, Koji M. Nishiguchi, Masayuki Yasuda, Yuji Tanaka, Kota Sato, Orie Nakamura, Kazuichi Maruyama, Toru Nakazawa

    BRAIN RESEARCH　1586　203-212　2014/10

    DOI： 10.1016/j.brainres.2014.08.053 　

    ISSN： 0006-8993

    eISSN： 1872-6240

  29. Critical Neuroprotective Roles of Heme Oxygenase-1 Induction Against Axonal Injury-Induced Retinal Ganglion Cell Death Peer-reviewed

    Noriko Himori, Kazuichi Maruyama, Kotaro Yamamoto, Masayuki Yasuda, Morin Ryu, Kazuko Omodaka, Yukihiro Shiga, Yuji Tanaka, Toru Nakazawa

    JOURNAL OF NEUROSCIENCE RESEARCH　92　(9)　1134-1142　2014/09

    DOI： 10.1002/jnr.23398 　

    ISSN： 0360-4012

    eISSN： 1097-4547

  30. Relationship of Skin Autofluorescence to Severity of Retinopathy in Type 2 Diabetes Peer-reviewed

    Masayuki Yasuda, Masahika Shimura, Hiroshi Kunikata, Hiroshi Kanazawa, Kanako Yasuda, Yuji Tanaka, Hideyuki Konno, Mai Takahashi, Taiki Kokubun, Kazuichi Maruyama, Noriko Sato, Masako Kakizaki, Mari Sato, Ichiro Tsuji, Toshio Miyata, Toru Nakazawa

    Current Eye Research　40　(3)　338-345　2014/05

  31. Profiles of Extracellular miRNAs in the Aqueous Humor of Glaucoma Patients Assessed with a Microarray System Peer-reviewed

    Yuji Tanaka, Satoru Tsuda, Hiroshi Kunikata, Junko Sato, Taiki Kokubun, Masayuki Yasuda, Koji M. Nishiguchi, Toshifumi Inada, Toru Nakazawa

    SCIENTIFIC REPORTS　4　5089　2014/05

    DOI： 10.1038/srep05089 　

    ISSN： 2045-2322

  32. RNA Sequence Reveals Mouse Retinal Transcriptome Changes Early after Axonal Injury Peer-reviewed

    Masayuki Yasuda, Yuji Tanaka, Morin Ryu, Satoru Tsuda, Toru Nakazawa

    PLOS ONE　9　(3)　e93258　2014/03

    DOI： 10.1371/journal.pone.0093258 　

    ISSN： 1932-6203

  33. Eyeball Deformation Characteristics during Air Jet Application Before and After the Vitreous Surgery Peer-reviewed

    UEMURA Shohei, KANEKO Makoto, AIZAWA Naoko, TANAKA Yuji, KUNIKATA Hiroshi, NAKAZAWA Toru

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME　2014　(0)　395-396　2014

    Publisher: The Japan Society of Mechanical Engineers

    DOI： 10.1299/jsmebio.2014.26.395 　

  34. A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes Peer-reviewed

    Kenta Nakagawa, Heather Harper-Lovelady, Yuji Tanaka, Masahito Tanaka, Masayuki Yamato, Toru Asahi

    CHEMICAL COMMUNICATIONS　50　(95)　15086-15089　2014

    DOI： 10.1039/c3cc49328h 　

    ISSN： 1359-7345

    eISSN： 1364-548X

  35. Development of azo-based fluorescent probes to detect different levels of hypoxia Peer-reviewed

    Wen Piao, Satoru Tsuda, Yuji Tanaka, Satoshi Maeda, Fengyi Liu, Shodai Takahashi, Yu Kushida, Toru Komatsu, Tasuku Ueno, Takuya Terai, Toru Nakazawa, Masanobu Uchiyama, Keiji Morokuma, Tetsuo Nagano, Kenjiro Hanaoka

    Angewandte Chemie - International Edition　52　(49)　13028-13032　2013/12/02

    DOI： 10.1002/anie.201305784 　

    ISSN： 1433-7851 1521-3773

  36. Intraocular concentrations of cytokines and chemokines in rhegmatogenous retinal detachment and the effect of intravitreal triamcinolone acetonide. International-journal Peer-reviewed

    Hiroshi Kunikata, Masayuki Yasuda, Naoko Aizawa, Yuji Tanaka, Toshiaki Abe, Toru Nakazawa

    American journal of ophthalmology　155　(6)　1028-1037　2013/06

    DOI： 10.1016/j.ajo.2013.01.013 　

    ISSN： 0002-9394

    More details Close

    PURPOSE: To investigate the role of intravitreal injection of triamcinolone acetonide (IVTA) in preventing photoreceptor apoptosis in eyes with rhegmatogenous retinal detachment (RRD) by measuring cytokine levels in the aqueous humor before and after IVTA. DESIGN: Prospective, nonrandomized, interventional case series. METHODS: setting: Institutional. patients: Nineteen eyes of 19 consecutive patients with RRD. intervention: All 19 eyes underwent IVTA 1 day before 25-gauge vitrectomy. Seventeen eyes free of retinal vascular disease served as controls. main outcome measure: Both baseline and 1 day post-IVTA measurements were made of the relative concentrations of 15 soluble factors (3 cytokines, 7 chemokines, and 5 growth factors). The associations with clinical findings, including macular status, were then analyzed. RESULTS: Elevated monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein 1β (MIP-1β), and interferon γ-induced protein 10 (IP-10) in eyes with RRD were significantly reduced after IVTA. MCP-1 levels were significantly correlated with MIP-1β and IP-10 before and after IVTA. The decreases in MCP-1, MIP-1β, and IP-10 were also closely correlated to each other. Both before and after IVTA, MCP-1 was higher in eyes with macula-off RRD than in eyes with macula-on RRD. CONCLUSIONS: IVTA suppressed elevated levels of intraocular MCP-1, MIP-1β, and IP-10 in eyes with RRD. The decrease in the aqueous levels of each of these factors was significantly correlated with the others. In addition to MCP-1, MIP-1β and IP-10 might potentially be additional target molecules for RRD therapy.

  37. Mechanical Characteristic Index Expressing Internal Properties of Eyeball Peer-reviewed

    KIMURA Yosuke

    TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series C　79　(799)　450-458　2013

    Publisher: The Japan Society of Mechanical Engineers

    DOI： 10.1299/kikaic.79.450 　

    ISSN： 0387-5024

  38. Metabolic stress response implicated in diabetic retinopathy: The role of calpain, and the therapeutic impact of calpain inhibitor Peer-reviewed

    Ahmed Y. Shanab, Toru Nakazawa, Morin Ryu, Yuji Tanaka, Noriko Himori, Keiko Taguchi, Masayuki Yasuda, Ryo Watanabe, Jiro Takano, Takaomi Saido, Naoko Minegishi, Toshio Miyata, Toshiaki Abe, Masayuki Yamamoto

    NEUROBIOLOGY OF DISEASE　48　(3)　556-567　2012/12

    DOI： 10.1016/j.nbd.2012.07.025 　

    ISSN： 0969-9961

    eISSN： 1095-953X

  39. Optical mechanical refinement of human amniotic membrane by dehydration and cross-linking Peer-reviewed

    Yuji Tanaka, Akira Kubota, Shunji Yokokura, Masafumi Uematsu, Dong Shi, Masayuki Yamato, Teruo Okano, Andrew J. Quantock, Kohji Nishida

    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE　6　(9)　731-737　2012/10

    DOI： 10.1002/term.479 　

    ISSN： 1932-6254

  40. A Simple Stock of Optical Glasses for a Catastrophic Disaster: Eyewear Donations after the 2011 Pacific Coast Tohoku Earthquake Peer-reviewed

    Yuji Tanaka, Ayako Yamato, Naoko Yaji, Masayuki Yamato

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　227　(2)　93-95　2012/06

    DOI： 10.1620/tjem.227.93 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  41. Irreversible optical clearing of rabbit dermis for autogenic corneal stroma transplantation Peer-reviewed

    Yuji Tanaka, Dong Shi, Akira Kubota, Yoshimasa Takano, Nobuo Fuse, Masayuki Yamato, Teruo Okano, Kohji Nishida

    BIOMATERIALS　32　(28)　6764-6772　2011/10

    DOI： 10.1016/j.biomaterials.2011.05.081 　

    ISSN： 0142-9612

  42. A Novel Gelatin Hydrogel Carrier Sheet for Corneal Endothelial Transplantation Peer-reviewed

    Ryou Watanabe, Ryuhei Hayashi, Yu Kimura, Yuji Tanaka, Tomofumi Kageyama, Susumu Hara, Yasuhiko Tabata, Kohji Nishida

    TISSUE ENGINEERING PART A　17　(17-18)　2213-2219　2011/09

    DOI： 10.1089/ten.tea.2010.0568 　

    ISSN： 1937-3341

  43. A method for enhancing the ocular penetration of eye drops using nanoparticles of hydrolyzable dye Peer-reviewed

    Koichi Baba, Yuji Tanaka, Akira Kubota, Hitoshi Kasai, Shunji Yokokura, Hachiro Nakanishi, Kohji Nishida

    JOURNAL OF CONTROLLED RELEASE　153　(3)　278-287　2011/08

    DOI： 10.1016/j.jconrel.2011.04.019 　

    ISSN： 0168-3659

    eISSN： 1873-4995

  44. Physical and Specific Crosslinking of Collagen Fibers by Supramolecular Nanogelators Peer-reviewed

    Michiya Matsusaki, Ryotaro Amekawa, Masahiro Matsumoto, Yuji Tanaka, Akira Kubota, Kohji Nishida, Mitsuru Akashi

    ADVANCED MATERIALS　23　(26)　2957-+　2011/07

    DOI： 10.1002/adma.201101284 　

    ISSN： 0935-9648

  45. Transparent, tough collagen laminates prepared by oriented flow casting, multi-cyclic vitrification and chemical cross-linking Peer-reviewed

    Yuji Tanaka, Koichi Baba, Thomas J. Duncan, Akira Kubota, Toru Asahi, Andrew J. Quantock, Masayuki Yamato, Teruo Okano, Kohji Nishida

    BIOMATERIALS　32　(13)　3358-3366　2011/05

    DOI： 10.1016/j.biomaterials.2010.11.011 　

    ISSN： 0142-9612

    eISSN： 1878-5905

  46. Irreversible optical clearing of sclera by dehydration and cross-linking Peer-reviewed

    Yuji Tanaka, Akira Kubota, Masayuki Yamato, Teruo Okano, Kohji Nishida

    BIOMATERIALS　32　(4)　1080-1090　2011/02

    DOI： 10.1016/j.biomaterials.2010.10.002 　

    ISSN： 0142-9612

  47. Anisotropic Mechanical Properties of Collagen Hydrogels Induced by Uniaxial-Flow for Ocular Applications Peer-reviewed

    Yuji Tanaka, Akira Kubota, Michiya Matsusaki, Thomas Duncan, Yoshikiyo Hatakeyama, Katsuya Fukuyama, Andrew J. Quantock, Masayuki Yamato, Mitsuru Akashi, Kohji Nishida

    JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION　22　(11)　1427-1442　2011

    DOI： 10.1163/092050610X510542 　

    ISSN： 0920-5063

    eISSN： 1568-5624

  48. Flow-manipulated, crosslinked collagen gels for use as corneal equivalents Peer-reviewed

    Thomas J. Duncan, Yuji Tanaka, Dong Shi, Akira Kubota, Andrew J. Quantock, Kohji Nishida

    BIOMATERIALS　31　(34)　8996-9005　2010/12

    DOI： 10.1016/j.biomaterials.2010.08.042 　

    ISSN： 0142-9612

  49. Site-Dependent Differences in Collagen Lamellae in the Corneal Substantia Propria of Beagle Dogs Peer-reviewed

    Aya Nagayasu, Tomoe Hirayanagi, Yuji Tanaka, Prasarn Tangkawattana, Hiromi Ueda, Kazushige Takehana

    JOURNAL OF VETERINARY MEDICAL SCIENCE　71　(9)　1229-1231　2009/09

    ISSN： 0916-7250

  50. Development of layered collagen gel with orthogonal molecular orientation

    Chizuru Hongo, Michiya Matsusaki, Yuji Tanaka, Kohji Nishida, Mitsuru Akashi

    8th World Biomaterials Congress 2008　3　1190　2008

Show all ︎Show first 5

Misc. 23

  1. 健康長寿多角的実測調査「YHAB」対象者の認知機能評価と関連指標の探索

    田中 佑治, YHAB研究グループ

    Dementia Japan　37　(4)　698-698　2023/10

    Publisher: (一社)日本認知症学会

    ISSN： 1342-646X

  2. 健康な後期高齢者32名中16名で疑われた睡眠時無呼吸症候群と罹患予測の可能性

    田中 佑治, 安藤 隆, 望月 和樹, 五十嵐 賢

    日本老年医学会雑誌　59　(Suppl.)　112-113　2022/05

    Publisher: (一社)日本老年医学会

    ISSN： 0300-9173

  3. ヒトiPS細胞由来RPE細胞紐状凝集塊移植の試み

    梶田 敬介, 西田 光宏, 田中 佑治, 田中 陽, 天谷 諭, 田中 信行, 宇山 紘史, 横田 聡, 三浦 央子, 栗本 康夫, 高橋 政代, 万代 道子, 理研・生命機能科学研究センター網膜再生医療研究開発プロジェクト

    日本眼科学会雑誌　126　(臨増)　202-202　2022/03

    Publisher: (公財)日本眼科学会

    ISSN： 0029-0203

  4. 睡眠時無呼吸症候群の危険因子としてのロコモティブシンドローム 山梨健康長寿コホートY-HALE study

    安藤 隆, 田中 佑治, 望月 和樹, 土屋 恭一郎, 谷口 直史, 落合 聡司, 萩野 哲男, 山縣 然太朗, 波呂 浩孝

    日本整形外科学会雑誌　96　(3)　S913-S913　2022/03

    Publisher: (公社)日本整形外科学会

    ISSN： 0021-5325

    eISSN： 2758-7479

  5. Matrix Quest 2019 生体組織透明化による角膜実質代替物の開発と検証

    田中 佑治

    日本結合組織学会学術大会プログラム・抄録集　51回　68-68　2019/05

    Publisher: 日本結合組織学会

  6. ヒトiPS細胞由来網膜色素上皮細胞懸濁液の未凍結条件下における保存の検討

    北畑 将平, 田中 佑治, 高橋 政代

    組織培養研究　37　(1)　38-38　2018/05

    Publisher: 日本組織培養学会

    ISSN： 0912-3636

    eISSN： 1881-3704

  7. 糖尿病網膜症における強膜自発蛍光測定の有用性の検討

    安田 正幸, 國方 彦志, 相澤 奈帆子, 田中 佑治, 永富 良一, 中澤 徹

    眼科臨床紀要　9　(10)　853-854　2016/10

    Publisher: 眼科臨床紀要会

    ISSN： 1882-5176

  8. Recurrent transcriptome alterations across multiple cancer types

    Bogumil Kaczkowski, Yuji Tanaka, Hideya Kawaji, Albin Sandelin, Robin Andersson, Masayoshi Itot, Timo Lassmann, Yoshihide Hayashizaki, Piero Carninci, Alistair R. R. Forrest

    CANCER RESEARCH　76　2016/07

    DOI： 10.1158/1538-7445.AM2016-2897 　

    ISSN： 0008-5472

    eISSN： 1538-7445

  9. ラット視神経挫滅モデルの網膜トランスクリプトーム解析

    安田 正幸, 西口 康二, 面高 宗子, 田中 佑治, 中澤 徹

    日本眼科学会雑誌　120　(臨増)　246-246　2016/03

    Publisher: (公財)日本眼科学会

    ISSN： 0029-0203

  10. マウス視神経挫滅モデルにおけるScotopic Threshold Response早期変化

    雪田 昌克, 町田 繁樹, 西口 康二, 津田 聡, 田中 佑治, 横山 悠, 安田 正幸, 丸山 和一, 中澤 徹

    眼科臨床紀要　8　(4)　251-251　2015/04

    Publisher: 眼科臨床紀要会

    ISSN： 1882-5176

  11. Aiming for Zero Blindness

    中澤徹, 國方彦志, 横倉俊二, 丸山和一, 西口康二, 高橋秀肇, 国松志保, 劉孟林, 檜森紀子, 田中佑治, 新田文彦, 森藤暁, 藤田幸輔, 中村織江, 面高宗子, 安田正幸, 横山悠, 相澤奈帆子, 山本耕太郎, 雪田昌克, 津田聡, 志賀由己浩, 前川重人, 國分太貴, 今野瑛之, 高橋麻衣, 萱場寛子, 橋本清香, 佐藤茉莉華, 矢花武史, 高田菜生子, 高橋世理, 鎌田泰彰, 秋葉正博, 木川勉, 松本朗子, 新家眞, 石橋達朗, 立花崇, 岩瀬愛子

    日本眼科学会雑誌　119　(3)　168-193　2015/03/10

    Publisher: 日本眼科学会

    ISSN： 0029-0203

  12. RNAシークエンスを用いたラット視神経軸索障害モデルの網膜トランスクリプトーム解析

    安田 正幸, 西口 康二, 面高 宗子, 田中 佑治, 中澤 徹

    日本眼科学会雑誌　119　(臨増)　242-242　2015/03

    Publisher: (公財)日本眼科学会

    ISSN： 0029-0203

  13. 糖尿病網膜症における強膜自発蛍光測定の有用性の検討

    安田正幸, 國方彦志, 相澤奈帆子, 田中佑治, 永富良一, 中澤徹

    日本網膜硝子体学会総会プログラム・講演抄録集　54th　85　2015

  14. In vivo imagingによる視神経挫滅後の網膜神経節細胞死の評価

    津田 聡, 田中 佑治, 國方 彦志, 横山 悠, 安田 正幸, 中澤 徹

    日本緑内障学会抄録集　25回　115-115　2014/09

    Publisher: 日本緑内障学会

  15. 前眼部偏光光干渉断層計による濾過胞内の線維化組織の観察

    國分太貴, 津田聡, 山成正宏, 田中佑治, 志賀由己浩, 横山悠, 国松志保, 高橋秀肇, 丸山和一, 國方彦志, 中澤徹

    日本緑内障学会抄録集　25th　90-90　2014

    Publisher: 日本緑内障学会

  16. 緑内障に関連する前房水miRNAマーカーのマイクロアレイ解析

    津田 聡, 田中 佑治, 安田 正幸, 佐藤 順子, 國分 太貴, 中澤 徹

    日本緑内障学会抄録集　24回　51-51　2013/09

    Publisher: 日本緑内障学会

  17. RNA-Seqによるマウス視神経挫滅後早期における網膜の網羅的遺伝子発現定量解析

    田中 佑治, 安田 正幸, 中澤 徹

    日本緑内障学会抄録集　24回　51-51　2013/09

    Publisher: 日本緑内障学会

  18. Development of neuroprotective drugs for glaucoma

    田中佑治, 中澤徹

    眼科　55　(5)　601-607　2013/05

    Publisher: 金原出版

    ISSN： 0016-4488

  19. 低酸素応答生理活性蛍光プローブを用いた網膜低酸素領域のin vivoイメージング

    津田聡, 田中佑治, 朴文, 國方彦志, 横山悠, 安田正幸, 花岡健二郎, 長野哲雄, 中澤徹

    日本眼科学会雑誌　117　(臨増)　280-280　2013/03/04

    Publisher: (公財)日本眼科学会

    ISSN： 0029-0203

  20. エリスロポエチンノックアウトマウスを用いた網膜・視神経の組織学的検討

    安田 正幸, 劉 孟林, 田中 佑治, 檜森 紀子, 面高 宗子, 横山 悠, 雪田 昌克, 津田 聡, 丸山 和一, 山本 雅之, 中澤 徹

    日本眼科学会雑誌　117　(臨増)　311-311　2013/03

    Publisher: (公財)日本眼科学会

    ISSN： 0029-0203

  21. 酸化ストレスによる網膜神経節細胞死におけるカルパインの働き

    横山 悠, 劉 孟林, 山本 耕太郎, 安田 正幸, 田中 佑治, 丸山 和一, 中澤 徹

    日本眼科学会雑誌　117　(臨増)　332-332　2013/03

    Publisher: (公財)日本眼科学会

    ISSN： 0029-0203

  22. Mechanical Characteristic Index Expressing Internal Properties of Eyeball

    木村洋介, 金子真, 相澤奈帆子, 田中佑治, 國方彦志, 中澤徹

    日本機械学会論文集 C編(Web)　79　(799)　450-458 (WEB ONLY)-458　2013

    Publisher: 一般社団法人 日本機械学会

    DOI： 10.1299/kikaic.79.450 　

    ISSN： 1884-8354

    More details Close

    There is a big need for estimating internal properties of eyeball, since they shoud be closely related to eye diseases, such as detached retina. Based on this background, this paper discusses a new bio-marker expressing the mechanical characteristics of internal eyeball. For this purpose, we focus on subjects having vitreum surgery in which vitreum of subject is replaced with succedaneum. With the assistance of high speed camera, the eye deformations before and after operation are captured during air-puff impartment. Through the analysis, we found the difference between the behaviors before and after operation, especially the shape of eye ball immediately after the tip of cornea recovers. We discuss why such different behaviors happen and how we can explain the physics.

  23. アポリポ蛋白質E欠損マウスにおけるカイニン酸障害および軸索障害に対する網膜神経節細胞の保護効果

    田中 佑治, Ahmed Shanab, 安田 正幸, 面高 宗子, 横山 悠, 劉 孟林, 中澤 徹

    日本緑内障学会抄録集　23回　152-152　2012/09

    Publisher: 日本緑内障学会

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Industrial Property Rights 27

  1. COMPOSITE INCLUDING NEURAL RETINA, RETINAL PIGMENT EPITHELIAL CELLS, AND HYDROGEL, AND METHOD FOR PRODUCING SAME

    Yuji TANAKA, Michiko MANDAI, Masayo TAKAHASHI, Suguru YAMASAKI

    特許7553924

    Property Type: Patent

    Holder:

  2. 包埋用ゲルでの生体試料の包埋を補助するための空間を備える医療用デバイス

    田中 佑治

    特許7458568

    Property Type: Patent

    Holder: 国立大学法人東北大学

  3. Medical treatment method, medical treatment apparatus, and medical treatment system for bloodstream disorder

    Property Type: Patent

  4. 認知機能判定プログラム、認知機能判定方法及び認知機能判定装置

    田中 佑治, 望月 和樹, 齋藤 光象, 土屋 恭一郎, 岩田 祐輔, 安藤 隆

    Property Type: Patent

  5. 鋳型核酸の分析方法、標的物質の分析方法、鋳型核酸または標的物質の分析用キット、および鋳型核酸または標的物質の分析用装置

    田中 佑治, 林崎 良英, 辻丸 光一郎

    Property Type: Patent

  6. 子宮体がんのリンパ節転移能の評価方法

    寺尾 泰久, 吉田 惠美子, 竹田 省, 林崎 良英, 伊藤 昌可, 川路 英哉, 田中 佑治

    Property Type: Patent

  7. 視神経障害発症リスクの判定方法

    田中 佑治, 安田 正幸, 面高 宗子, 中澤 徹

    Property Type: Patent

  8. 蛍光測定装置及び蛍光測定方法

    目賀 章正, 田中 佑治, 安田 正幸, 永富 良一, 中澤 徹

    Property Type: Patent

  9. 透明化羊膜の作製方法及び透明化羊膜

    西田 幸二, 田中 佑治, 久保田 享, 横倉 俊二, 上松 聖典

    Property Type: Patent

  10. 皮膚真皮透明化による角膜移植材料調製法

    西田 幸二, 田中 佑治, 久保田 亨

    Property Type: Patent

  11. 角膜実質細胞培養用スキャフォールド

    西田 幸二, 田中 佑治, 久保田 享, 石 棟

    Property Type: Patent

  12. 高分子薄膜、高分子積層膜の製造方法、その製造方法で製造された高分子薄膜、および高分子積層膜

    田中 佑治, 大和 雅之, 岡野 光夫, 西田 幸二, 馬場 耕一, 久保田 享, 朝日 透, 中川 鉄馬

    Property Type: Patent

  13. 眼内移行性の高い点眼剤および蛍光イメージング剤ならびにそれらの製造方法

    西田 幸二, 馬場 耕一, 笠井 均, 田中 佑治, 久保田 享, 横倉 俊二

    Property Type: Patent

  14. 強膜透明化による角膜移植材料調製方法

    西田 幸二, 田中 佑治, 久保田 享

    Property Type: Patent

  15. 強膜透明化による角膜移植材料調製方法

    西田 幸二, 田中 佑治, 久保田 享

    特許第5388090号

    Property Type: Patent

  16. 角膜経上皮電気抵抗値の測定方法

    上松 聖典, 北岡 隆, 西田 幸二, 田中 佑治, 西澤 松彦, 梶 弘和, 関根 宗一郎

    Property Type: Patent

  17. 積層コラーゲンゲルの作製方法及び積層コラーゲンゲル

    明石 満, 西田 幸二, 松崎 典弥, 本郷 千鶴, 田中 佑治, 久保田 享

    Property Type: Patent

  18. ⽣細胞を保存するための⽅法

    田中佑治

    Property Type: Patent

  19. ⽣体試料製造デバイス、それに⽤いる剥離器具、及び⽣体試料製造⽅法

    田中佑治, 市川比奈子

    Property Type: Patent

  20. 認知機能判定プログラム、認知機能判定方法及び認知機能判定装置

    田中佑治

    Property Type: Patent

  21. 網膜色素上皮細胞のひも状凝集体、それを製造するためのデバイスおよび製造方法、ならびに該ひも状凝集体を含有する治療薬

    高橋政代, 田中佑治, 田中陽, 田中信行, 天谷論, 西田光宏, 万代道子

    Property Type: Patent

  22. 温度制御により薬効解消可能な眼圧上昇剤

    田中佑治, 柏木賢治, 大澤重仁

    Property Type: Patent

  23. 生体試料の培養と包埋材での生体試料の包埋を行う医療用デバイス、キット及び培養包埋方法

    田中佑治

    Property Type: Patent

    Holder: 国立大学法人東北大学

  24. 蛍光測定装置及び蛍光測定方法

    目賀 章正, 田中 佑治, 安田 正幸, 永富 良一, 中澤 徹

    特許第6161693号

    Property Type: Patent

  25. 眼内移行性の高い点眼剤および蛍光イメージング剤ならびにそれらの製造方法

    西田 幸二, 馬場 耕一, 笠井 均, 田中 佑治, 久保田 享, 横倉 俊二

    特許第5709523号

    Property Type: Patent

  26. 角膜経上皮電気抵抗値の測定方法及び装置

    上松 聖典, 北岡 隆, 西田 幸二, 田中 佑治, 西澤 松彦, 梶 弘和, 関根 宗一郎

    特許第5470508号

    Property Type: Patent

  27. 積層コラーゲンゲルの作製方法及び積層コラーゲンゲル

    明石 満, 西田 幸二, 松崎 典弥, 本郷 千鶴, 田中 佑治, 久保田 享

    特許第5525823号

    Property Type: Patent

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Research Projects 20

  1. 非凍結低温保存『冷眠』技術の確立と工程横断型細胞死制御による複合網膜移植の実装研究

    田中佑治, 市川比奈子, 金野智弘, 黒田拓也

    Offer Organization: AMED

    System: 再生・細胞医療・遺伝子治療実現加速化プログラム / 再生・細胞医療・遺伝子治療研究開発課題（基礎応用研 究課題）

    Institution: 東北大学、横浜市立大学、国立医薬品食品衛生研究所

    2026/08 - 2029/03

  2. 次世代網膜再生医療のための製造基盤及び臨床パイプライン開発のための事業化基盤の構築

    Offer Organization: JST

    System: 令和8年度みちのくGAPファンドSTEP2

    2026/07 - 2029/03

  3. 高齢運転者の事故リスク可視化に資する睡眠時無呼吸症候群・認知機能・運転実態等の調査データ基盤強化と社会実装

    Offer Organization: 一般社団法人 日本損害保険協会

    System: 自賠責運用益拠出事業

    Institution: 東北大学、国立長寿医療研究センター

    2026/04 - 2029/03

  4. 高齢者の眠りに潜む事故リスクの可視化技術の開発と安心・安全社会の提案

    Offer Organization: 国立研究開発法人新エネルギー・産業技術総合開発機構

    System: 官民による若手研究者発掘支援事業（共同研究フェーズ）

    Institution: 国立大学法人東北大学、国立研究開発法人国立長寿医療研究センター

    2025/09 - 2027/09

  5. 温度応答性整体親和性ポリマーを用いた新しい眼科治療法の開発（仮）

    Offer Organization: AMED橋渡し研究筑波大学拠点

    System: 橋渡し研究シーズA

    Institution: 東北大学

    2025/04 - 2027/03

  6. 温度応答性ポリマーを用いた新しい細胞培養方法の開発（仮）

    Offer Organization: 池谷科学技術振興財団

    2025/04 - 2026/03

  7. 複合細胞膜One-pot形成・保存キットの事業化検証

    Offer Organization: 東北大学

    System: BIPギャップファンド

    Institution: 東北大学

    2024/08 - 2025/03

  8. 低眼圧症治療のための冷却溶解性隅角閉塞材の開発

    田中佑治

    Offer Organization: AMED

    System: 橋渡し研究プログラムpreF

    2023/04 - 2025/03

  9. A proposal for "anti-dementia dietary habits" based on healthy longevity epigenomics that links dietary habits and cognitive function

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: University of Yamanashi

    2021/04 - 2024/03

  10. 世界に先駆けた代謝異常に起因するサルコペニア動物モデルの樹立とヒトによる検証

    望月 和樹, 安藤 隆, 田中 佑治

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的研究(萌芽)

    Category: 挑戦的研究(萌芽)

    Institution: 山梨大学

    2020/07/30 - 2023/03/31

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    1. WTマウスと老齢サルコペニアモデルBrd4ヘテロ欠損マウスを比較したところ、対照食摂取群において、Brd4ヘテロ欠損マウスの体重、握力、臓器重量(肝臓、副睾丸脂肪、腓腹筋、ヒラメ筋、前脛骨筋)が顕著に低く、サルコペニアマウスとして使用できる可能性が示唆された。 2. 昨年度まで、カプリル酸が多い油脂[カプリル酸(C8)：カプリン酸(C10)＝75%:25%]を使用していたが、今年度は、カプリン酸が多い油脂[カプリル酸(C8)：カプリン酸(C10)＝25%:75%]を投与する実験を行った。具体的には、WT マウスとBrd4ヘテロ欠損マウスに対照食（長鎖飽和脂肪が多いラードを油脂源として使用）およびカプリン酸が多い油脂[カプリル酸(C8)：カプリン酸(C10)＝25%:75%]に置き換えた食餌を5ヶ月のあいだ投与した。その結果、中鎖脂肪が多い食餌を摂取したBrd4ヘテロ欠損マウスでは体重や副睾丸脂肪は低下傾向を示したが、腓腹筋、ヒラメ筋、前脛骨筋、長指伸筋の重量は増大傾向を示した。さらにBrd4ヘテロ欠損マウスへの中鎖脂肪の投与により握力が顕著に上昇した。

  11. ロボット指導、見守り等の実証研究

    Offer Organization: ロボット指導、見守り等の実証研究

    System: Society5.0実現化研究拠点支援事業 ライフデザイン・イノベーション研究拠点グランドチャレンジ

    2022/07 - 2023/03

  12. 健康寿命の長い高齢者 の PLR 取得基盤の形成

    Offer Organization: 文部科学省

    System: Society 5.0 実現化 研究拠点支援事業 (ライフデザイン・ イノベーション研究 拠点)

    Category: グランドチェレンジ

    2020/04 - 2022/03

  13. 網膜への薬剤送達を目指したバリア突破型温度応答性スマートナノ点眼の開発

    田中 佑治, 大澤 重仁

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的研究(萌芽)

    Category: 挑戦的研究(萌芽)

    Institution: 山梨大学

    2018/06/29 - 2020/03/31

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    2020年度は、前年度mRNA導入の効果検証系として選択したマウス視神経挫滅モデルへの神経保護物質の眼内注射実験系の検証を継続した。まず正常コントロールとして、細胞死抑制効果が報告されているROCK inhibitorまたは生理食塩水を視神経挫滅後のマウス眼内に注射した。網膜神経節細胞（RGC）数の評価方法として、処置5日後に摘出した網膜を展開して、代表的なRGCマーカーであるBrn3aとRbpmsを免疫染色した。未処置ガンに対する傾向陽性細胞の割合は、Brn3a染色では、生理食塩水で54.3％であったのに対して、ROCK inhibitor 注射眼が66.5%、Rbpms染色では、生理食塩水で48.5％であったのに対して、ROCK inhibitor 注射眼が60.3%と、双方共にROCK inhibitor注射眼でRGC保護効果が認められ、神経保護効果を評価できることを確認した。しかしながら、RGCに対する神経保護効果が報告されているBdnfのmRNAを内包したナノミセルを上記の視神経挫滅モデルに注射したが神経保護効果は認められなかった。そこでナノミセルを構成する生体親和性ポリマーを新たに検討して従来と異なるものを複数用意した。また、培養系での検証への移行の可能性を検証するために、網膜色素上皮細胞の細胞死保護効果検証系のFeasibilityを検証した。さらに。この生体親和性ポリマーを活用した新しい眼科治療法の提案をmRNAの導入に囚われずに検討して、眼圧制御を効果的に実現する新しいコンセプトの試薬の提案に繋げて特許出願や新しい予算申請等に繋げた。

  14. Development of Digital Isothermal Nucleic Acid Amplification for Rapid Quantification

    Tanaka Yuji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    2016/04/01 - 2018/03/31

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    In this study, we examined two new detection methods: digital nucleic acid quantification method with reverse transcription and digital nucleic acid quantification method of identifying and quantifying nucleic acid amplified by melting curve analysis. The isothermal amplification method can amplify a nucleic acid only by maintaining it at around 70 ° C, but there is a problem that it can not be determined unless there is a 10-fold concentration difference, but digitization of the isothermal amplification kit with reverse transcription It has been found that it becomes possible to detect even a concentration difference of about 2 times. In addition, in order to detect genomic mutations used for quality control of cells utilized for regenerative medicine of retina, newly designed primers for isothermal amplification, digital nucleic acid amplification, and biotyped and mutated nucleic acid by melting curve analysis. It turned out that it can be identified and quantified.

  15. Research for blood RNA marker predicting prognosis of glaucoma focusing on the early and individualized medicine.

    Nakazawa Toru, ITO Masayoshi, KAWAJI Hideya

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    Glaucoma is a chronic ocular disease that is often influenced by high intraocular pressure, resulting in irreversible loss of visual field. Visual field loss caused by glaucoma is progressive, thus, we should diagnose glaucoma at its early stages and start the treatment if indicated. The goal of the present study is to identify biomarkers for glaucoma, through investigating the translational profiles of RNA derived from blood. Thirty-two patients with normal tension glaucoma and 32 controls with ocular diseases which have little association with genetic factors were recruited in the present study. RNA was extracted from the peripheral blood. Activation of promoters of all the genes were comprehensively quantitated using cap analysis gene expression (CAGE) technique. We selected the candidate genes as biomarker according to the result of the differential analysis of the two groups. The promoters identified hold promise for their clinical application as biomarkers for glaucoma.

  16. Experimental application of piezoelectric actuator-driven pulsed water jets in retinal vein occlusion surgery

    Kunikata Hiroshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    We found that at 0.025-ml/min, IOP stayed below 40 mmHg even after 3 min. Moreover, we found that a 0.025 ml/min ADPJ stream was stable at a pulse frequency of 10Hz, and that at this flow rate/frequency the ADPJ pressure was closely correlated with the applied voltage. The minimum voltage and distance to achieve intravenous displacement of the blood column (DBC) without causing retinal tearing or hemorrhage were 40 V and 0.5 mm, respectively. Furthermore, at this voltage (40V), consecutive OCT measurements revealed that this inner retinal swelling resolved within 1 week. Histological examination of the enucleated eyes 7 days after ADPJ massage showed that retinal areas near the massaged vessels were similar to areas near untreated vessels. With an appropriate flow rate and surgical time, ADPJ successfully induced massage of the retinal vessels and intravenous DBC while maintaining safe IOP and not causing retinal complications.

  17. Search for RNA Biomarker in Aqueous Humor from patients by Conprehensive Transcriptome Analysis

    Tanaka Yuji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Institute of Physical and Chemical Research

    2014/04/01 - 2017/03/31

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    We extracted a trace amount of RNA from anterior aqueous humor from patients with ophthalmopathy of just a few 100 μL and tried comprehensive RNA analysis using next generation sequencer etc. About 360 kinds of microRNAs were successfully detected from one clinical specimen, and about one third of them were commonly detected in eight cataract specimens. Of these, 75% of the top 40 in expression levels were consistent with other studies reported during the study period. As a study that tried comprehensive analysis individually without mixing samples, our microarray analysis reported at the beginning of the research period is the first time, and the analysis scale by the next generation sequencer is the largest. In addition, as a surrounding study, we have examined microRNA analysis method and pathology model evaluation method and obtained basic knowledge.

  18. Development of novel device for ocular surface treatment with tranparent amnion membrane

    Yokokura Shunji, TANAKA YUJI, WATANABE RYO

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    We made alternative corneal stroma having strenght and tranparency the same as normal corneal stroma with transparent human amniotic membrane laminates. When these laminates were tranplanted to rabbit cornea, these membranes survived for a long time. These laminates were useful for filling scleral defect and covering glaucoma shunt device. We manipulated scleral contact lens with these laminates. When this contact lens was worn on chemical injured rabbit cornea, inflammation on the ocular surface was reduced significantly. This contact lens might be effective for severe ocular surface inflammation.

  19. Development of retinal treatment with dental pulp cells

    NAKAZAWA Toru, TANAKA Yuji, SASAKI Ryo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2012/04/01 - 2014/03/31

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    The transplantation of dental pulp-derived cells to the eye was explored for the possibility of the novel neuroprotection and regenerative treatments to the critical retinal diseases that results in vision loss. Firstly, the isolated dental pulp-derived cells were directly injected into normal rat vitreous, and it was confirmed that some of the injected cells adhered to the retina and formed the neuronal process. Furthermore, several cell manipulation methods were examined to enrich stem cell population and up-regulate neuron-related molecular expression. Specifically, (1) gene expression of nerve and stem cell related molecule were activates by sphere-forming floating culture, (2) sphere-forming cells can be enriched by cell sorting of CD105-positive dental pulp-derived cells, (3) the expression of an axonal regeneration related molecule was promoted by applying hypoxic culture to the sphere-forming culture.

  20. Development of opticaly-cleared tissues for novel ophthalmic treatments

    TANAKA Yuji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2012/04/01 - 2014/03/31

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    For the ophthalmic application of optically-cleared tissues, their modification and animal experiments were investigated in this study. This study focused on the amnion that were widely used in the ophthalmology field, and examined their biocompatibility in vivo and also improvement in the tolerance to a suture and transparency. Firstly, it was confirmed that no inadequate biological responses to the optically-cleared amniotic membrane were observed during the transplanted into rabbit corneal stroma. Next, further functional refinements of the optically-cleared amnion, and additional tissue optical clearing were also induced. Further optical-clearing was effective to improve the transparency of the refinement processes. Furthermore, the improvements of mechanical properties were confirmed that it was possible to improve the mechanical property to about 10 times of the original amnion, and that the corneal transplantation, which requires a tight suture, was also possible.

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Teaching Experience 1

  1. Special Education Program for Development of Integrated Science and Technology for Health and Longevity　University of Yamanashi

Media Coverage 9

  1. 高齢者の交通事故要因、睡眠との関連探る

    河北新報社

    2026/08/23

    Type: Newspaper, magazine

  2. AIロボ同居 健康に 独居高齢者の「話し相手」

    山梨日日新聞　1面見出し

    2023/03/06

    Type: Newspaper, magazine

  3. 「山梨県の健康長寿」

    FM-Fuji

    2022/12

    Type: TV or radio program

  4. 80歳以上の9割に症状 睡眠時無呼吸症候群を山梨大調査

    朝日新聞デジタル　https://www.asahi.com/articles/ASQCD6QZHQC7UZOB002.html

    2022/11

    Type: Internet

  5. 睡眠時無呼吸症候群 80歳以上の9割に

    朝日新聞　山梨県地域紙面

    2022/11

    Type: Newspaper, magazine

  6. 「元気な高齢者でも無呼吸症候群 注意」

    山梨日日新聞　1面

    2022/11

    Type: Newspaper, magazine

  7. 革新的イノベーション創出プログラムCOIで「何が実現でき 何が足りなかったか」

    日経BP　https://project.nikkeibp.co.jp/behealth/atcl/feature/00004/030100252/

    2021/02

    Type: Internet

  8. 新型コロナウイルス対策として入院患者モニタリングシステムを新規導入

    NHKなど　山梨日日新聞社など

    2020/12

    Type: TV or radio program

  9. 「山梨大と大阪大研究連携 健康長寿の理由 生活から探る」

    山梨日日新聞　1面見出し

    2020/06

    Type: Newspaper, magazine

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