Details of the Researcher

PHOTO

Keisuke Nakamura
Section
Graduate School of Dentistry
Job title
Professor
Degree

  • 博士（歯学）（東北大学）

Research History 10

  • 2025/04 - Present
    Tohoku University　Graduate School of Dentistry　Professor

  • 2023/09 - Present
    University of Gothenburg　Institute of Odontology

  • 2018/04 - 2025/03
    Tohoku University　Graduate School of Dentistry　Associate Professor

  • 2017/04 - 2018/03
    Tohoku University　Graduate School of Dentistry　Lecturer

  • 2011/04 - 2016/03
    Tohoku University　Graduate School of Dentistry　Assistant Professor

  • 2010/08 - 2011/03
    Tohoku University　New Industry Creation Hatchery Center　Assistant Professor

  • 2008/10 - 2010/07
    Tohoku University　New Industry Creation Hatchery Center　Research Fellow

  • 2007/10 - 2008/09
    University of Gothenburg　Institute of Odontology　Visiting Researcher

  • 2007/04 - 2007/09
    Tohoku University　New Industry Creation Hatchery Center　Research Fellow

  • 2006/04 - 2007/03
    Dental Clinic at Tohoku University Hospital　Intern Dentist

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Education 3

  • University of Gothenburg,　Sahlgrenska Academy (Ph.D. course)

    2009/09 - 2015/04

  • Tohoku University　Graduate School of Dentistry

    2002/04 - 2006/03

  • Tohoku University　School of Dentistry

    1996/04 - 2002/03

Research Areas 2

  • Life sciences / Prosthodontics /

  • Life sciences / Regenerative dentistry and dental engineering /

Awards 5

  1. Best Thesis 2015 at Institute of Odontology

    2016/05　University of Gothenburg,

  2. 第39回日本防菌防黴学会 ポスター賞

    2012/09　日本防菌防黴学会

  3. 奨励賞

    2012/05　（公財）インテリジェント・コスモス学術振興財団　過酸化水素光分解によるヒドロキシルラジカル生成技術を応用した新規歯周病治療器の開発

  4. Excellent Young Investigator Award

    2009/01　The 3rd International Symposium for Interface Oral Health Science,

  5. 学術講演会発表優秀賞受賞

    2004/04　日本歯科理工学会

Papers 91

  1. A dental laboratory survey of clinical failure rates in zirconia and conventional restorations over six years Peer-reviewed

    Omma Moonfalk, Keisuke Nakamura, Ulf Örtengren, Per Svanborg

    The Journal of Prosthetic Dentistry　2025/08

    DOI： 10.1016/j.prosdent.2025.07.011 　

  2. Therapeutic Potential of Proanthocyanidins in Dentistry: A Focus on Periodontal Disease and on Dental Implants in Osteoporotic Patients Peer-reviewed

    Yoshimi Niwano, Shunichi Shishido, Midori Shirato, Hidetsugu Kohzaki, Keisuke Nakamura

    Antioxidants　2025/07/10

    DOI： 10.3390/antiox14070850 　

  3. A triple growth factor strategy for optimizing bone augmentation in mice. International-journal Peer-reviewed

    Taichi Tenkumo, Rie Koide, Toru Ogawa, Hirofumi Yamaguchi, Shigeki Suzuki, Makiko Miyashita, Keisuke Nakamura, Han Wang, Nobuhiro Yoda, Keiichi Sasaki

    Journal of biomedical materials research. Part B, Applied biomaterials　112　(7)　e35447　2024/07

    DOI： 10.1002/jbm.b.35447 　

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    With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF). Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V). The prepared scaffolds were surgically implanted into the calvarias of C57BL/6JJcl mice, and hard tissue formation was assessed after 10 and 28 days through histological, tomographic, and biochemical analyses. The combination of BMP-2 and IGF-1 induced a greater volume of hard tissue augmentation compared with that of BMP-2 alone, regardless of VEGF supplementation, and these groups had increased levels of cartilage compared with others. The volume of hard tissue formation was greatest in the BIV group. In contrast, the BI + V group exhibited a hard tissue volume similar to that of the BI group. While VEGF and CD31 levels were highest in the BIV group at 10 days, there was no correlation at the same time point between hard tissue formation and the quantity of M2 macrophages. In conclusion, the simultaneous release of BMP-2, IGF-1, and VEGF proved to be effective in promoting bone augmentation.

  4. Critical evaluations on the crystallographic properties of translucent dental zirconia ceramics stabilized with 3-6 mol% yttria Peer-reviewed

    Keisuke Nakamura, Shunichi Shishido, Ryoichi Inagaki, Taro Kanno, Sargon Barkarmo, Per Svanborg, Ulf Örtengren

    Dental Materials　2024/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.dental.2024.06.027 　

    ISSN： 0109-5641

  5. Mechanism underlying ultraviolet-irradiation-induced discoloration of dental zirconia ceramics stabilized with 3 and 5 mol% yttria Peer-reviewed

    Akari Kurihara, Keisuke Nakamura, Shunichi Shishido, Ryoichi Inagaki, Akio Harada, Taro Kanno, Hiroshi Egusa

    Ceramics International　50　(7)　12136-12145　2024/04

    Publisher: Elsevier BV

    DOI： 10.1016/j.ceramint.2024.01.117 　

    ISSN： 0272-8842

  6. The formation of cariogenic plaque to contemporary adhesive restorative materials: an in vitro study Peer-reviewed

    Anna Lehrkinder, Olivia Rydholm, Anna Wänström, Keisuke Nakamura, Ulf Örtengren

    Odontology　2024/03/19

    DOI： 10.1007/s10266-024-00913-5 　

    ISSN： 1618-1247 1618-1255

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    <jats:title>Abstract</jats:title><jats:p>The research exploiting the ability of dental materials to induce or prevent secondary caries (SC) development still seems inconclusive. Controlling bacterial adhesion by releasing bacteriostatic ions and improving the surface structure has been suggested to reduce the occurrence of SC. This paper analyses the impact of five distinctively composed dental materials on cariogenic biofilm formation. Forty-five specimens of three composites (CeramX Spectra ST, Admira Fusion, Beautifil II) and two glass–ionomers (Fuji II LC, Caredyne Restore), respectively, were incubated in bacterial suspension composed of <jats:italic>Streptococcus mutans</jats:italic>, <jats:italic>Lactobacillus acidophilus</jats:italic>, <jats:italic>Streptococcus mitis, Streptococcus sanguinis, and Streptococcus salivarius</jats:italic> at pH 7.0 and 5.5. Coverslips were used as a control. Adhered bacteria were collected after 2, 4, 6, 12, 24, and 48 h and analyzed using quantitative polymerase chain reaction (qPCR). Fluoride leakage was measured at each collection. The specimens’ surface topography was assessed using interferometry. In the present study, surface roughness seemed to have a partial role in bacterial adhesion and biofilm formation, together with chemical composition of the materials tested. Despite differences in fluoride leakage, biofilm accumulation was similar across materials, but the number of adhered bacteria differed significantly. A release of other ions may also affect adhesion. These variations suggest that certain materials may be more prone to initiating secondary caries.</jats:p>

  7. 青色レーザーを応用した歯周病治療器「ブルーラジカルP-01」 Invited Peer-reviewed

    中村圭祐

    日本歯科理工学会誌　43　151-154　2024

  8. 歯科医療分野における微生物制御：4.歯周病と全身疾患の関係性 Invited Peer-reviewed

    天雲太一, 中村圭祐, 白土翠

    日本防菌防黴学会誌　52　315-321　2024

  9. 歯科医療分野における微生物制御：2.デンタルプラーク Invited Peer-reviewed

    日本防菌防黴学会誌　52　205-211　2024

  10. 歯科医療分野における微生物制御：1.開講にあたって Invited Peer-reviewed

    中村圭祐

    日本防菌防黴学会誌　52　169-171　2024

  11. Residual stress associated with crystalline phase transformation of 3-6 mol% yttria-stabilized zirconia ceramics induced by mechanical surface treatments. International-journal Peer-reviewed

    Shunichi Shishido, Ryoichi Inagaki, Taro Kanno, Per Svanborg, Sargon Barkarmo, Ulf Örtengren, Keisuke Nakamura

    Journal of the mechanical behavior of biomedical materials　146　106067-106067　2023/08/04

    DOI： 10.1016/j.jmbbm.2023.106067 　

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    Monolithic dental prostheses made of 3-6 mol% yttria-stabilized zirconia (3-6YSZ) have gained popularity owing to their improved material properties and semi-automated fabrication processes. In this study, we aimed to evaluate the influence of mechanical surface treatments, such as polishing, grinding, and sandblasting, on the residual stress of 3-6YSZ used for monolithic prostheses in association with crystalline phase transformation. Plate specimens were prepared from five dental zirconia blocks: Aadva Zirconia ST (3YSZ), Aadva Zirconia NT (6YSZ), Katana HT (4YSZ), Katana STML (5YSZ), and Katana UTML (6YSZ). The specimens were either polished using 1, 3, or 9 μm diamond suspensions, ground using 15, 35, or 55 μm diamond discs, or sandblasted at 0.2, 0.3, or 0.4 MPa. The residual stress, crystalline phase, and hardness were analyzed using the cosα method, X-ray diffraction (XRD), and Vickers hardness test, respectively. Additionally, we analyzed the residual stress on the surfaces of monolithic zirconia crowns (MZCs) made of 4YSZ, 5YSZ, and 6YSZ, which were processed using clinically relevant procedures, including manual grinding, followed by polishing using a dental electric motor on the external surface, and sandblasting on the internal surface. Residual stress analysis demonstrated that grinding and sandblasting, particularly the latter, resulted in the generation of compressive residual stress on the surfaces of the plate specimens. XRD revealed that the ground and sandblasted specimens contained a larger amount of the rhombohedral phase than that of the polished specimens, which may be a cause of the residual stress. Sandblasting significantly increased the Vickers hardness compared to polishing, which may possibly be due to the generation of compressive residual stress. In the case of MZCs, compressive residual stress was detected not only on the sandblasted surface, but also on the polished surface. The difference in the residual stress between the plate and crown specimens may be related to the force applied during the automated and manual grinding and polishing procedures. Further studies are required to elucidate the effects of the compressive residual stress on the clinical performance of MZCs.

  12. Mutagenicity assessment of high-power 1.6-THz pulse laser radiation. International-journal Peer-reviewed

    Midori Shirato, Yuma Takida, Taro Kanno, Hiroshi Matsuura, Yoshimi Niwano, Hiroaki Minamide, Keisuke Nakamura

    Photochemistry and photobiology　2023/07/21

    DOI： 10.1111/php.13840 　

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    The effect of terahertz (THz) radiation has been studied in medicine. However, there is a lack of scientific information regarding its possible mutagenicity. Therefore, the present study aimed to assess the mutagenicity of 1.6 THz laser irradiation. The Ames test was conducted using five bacterial tester strains. The bacteria were subjected to (i) 1.6 THz laser irradiation at 3.8 mW/cm2 for 60 min using a tabletop THz pulse laser system, (ii) ultraviolet irradiation, (iii) treatment with positive control chemicals (positive control) or (iv) treatment with the solvent used in the positive control (negative control). After treatment, the bacterial suspensions were cultured on minimal glucose agar to determine the number of revertant colonies. In addition, the comet assay was performed using fibroblasts (V79) to assess possible DNA damage caused by the THz laser irradiation. The Ames test demonstrated that the THz laser irradiation did not increase the number of revertant colonies compared to that in the negative control group, whereas the ultraviolet irradiation and positive control treatment increased the number of revertant colonies. Thus, 1.6 THz laser irradiation is unlikely to be mutagenic. The comet assay additionally suggests that the THz laser irradiation unlikely induce cellular DNA damage.

  13. Fabrication of Optical Fiber Bundles with Wire-Grid Polarizers and Its Application to Tooth Substance Discrimination Peer-reviewed

    Taiyu Okatani, Yuki Yokoyama, Shunichi Shishido, Keisuke Nakamura, Yoshiaki Kanamori

    IEEJ Transactions on Sensors and Micromachines　143　(7)　192-199　2023/07/01

    Publisher: Institute of Electrical Engineers of Japan (IEE Japan)

    DOI： 10.1541/ieejsmas.143.192 　

    ISSN： 1341-8939

    eISSN： 1347-5525

  14. Low-temperature degradation of yttria-stabilized zirconia treated with pulsed laser and annealing techniques Peer-reviewed

    Tomohiro Harai, Masayoshi Mizutani, Shunichi Shishido, Keisuke Nakamura, Hitoshi Ohmori, Toyohiko J. Konno, Tsunemoto Kuriyagawa

    Precision Engineering　80　45-56　2023/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.precisioneng.2022.11.010 　

    ISSN： 0141-6359

  15. Metabolic Fate of Orally Ingested Proanthocyanidins through the Digestive Tract Peer-reviewed

    Yoshimi Niwano, Hidetsugu Kohzaki, Midori Shirato, Shunichi Shishido, Keisuke Nakamura

    Antioxidants　12　(1)　17-17　2022/12/22

    Publisher: MDPI AG

    DOI： 10.3390/antiox12010017 　

    eISSN： 2076-3921

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    Proanthocyanidins (PACs), which are oligomers or polymers of flavan-3ols with potent antioxidative activity, are well known to exert a variety of beneficial health effects. Nonetheless, their bioaccessibility and bioavailability have been poorly assessed. In this review, we focused on the metabolic fate of PACs through the digestive tract. When oligomeric and polymeric PACs are orally ingested, a large portion of the PACs reach the colon, where a small portion is subjected to microbial degradation to phenolic acids and valerolactones, despite the possibility that slight depolymerization of PACs occurs in the stomach and small intestine. Valerolactones, as microbiota-generated catabolites of PACs, may contribute to some of the health benefits of orally ingested PACs. The remaining portion interacts with gut microbiota, resulting in improved microbial diversity and, thereby, contributing to improved health. For instance, an increased amount of beneficial gut bacteria (e.g., Akkermansia muciniphila and butyrate-producing bacteria) could ameliorate host metabolic functions, and a lowered ratio of Firmicutes/Bacteroidetes at the phylum level could mitigate obesity-related metabolic disorders.

  16. Inhibition of tooth demineralization caused by Streptococcus mutans biofilm via antimicrobial treatment using hydrogen peroxide photolysis. International-journal Peer-reviewed

    Midori Shirato, Keisuke Nakamura, Taichi Tenkumo, Yoshimi Niwano, Taro Kanno, Keiichi Sasaki, Peter Lingström, Ulf Örtengren

    Clinical oral investigations　27　(2)　739-750　2022/12/09

    DOI： 10.1007/s00784-022-04821-2 　

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    OBJECTIVES: An antimicrobial technique utilizing hydroxyl radicals generated by the photolysis of 3% H2O2 has been developed recently. The present study aimed to evaluate the effect of H2O2 photolysis treatment on tooth demineralization caused by Streptococcus mutans biofilm. MATERIALS AND METHODS: To induce tooth demineralization, S. mutans biofilm was allowed to form on the maxillary first molars collected from Wistar rats via 24-h culturing. The samples were immersed in 3% H2O2 and irradiated with 365-nm LED (H2O2 photolysis treatment). Viable bacterial counts in the biofilm were evaluated immediately after treatment and after an additional 30-h culturing by colony counting. The acidogenicity of the biofilm, re-established 30 h after treatment, was assessed by measuring the pH. The effect of H2O2 photolysis treatment on tooth demineralization was assessed by measuring the depth of the radiolucent layer in micro-CT images. RESULTS: H2O2 photolysis significantly reduced viable bacterial counts in the biofilm to 3.7 log colony forming units (CFU)/sample, while the untreated group had 7.9 log CFU/sample. The pH of the biofilm re-established after treatment (6.6) was higher than that of the untreated group (5.3). In line with the pH measurement, the treatment group had a significantly lower depth of radiolucent layer in dentin than the untreated group. CONCLUSIONS: H2O2 photolysis treatment was effective not only in killing the biofilm-forming S. mutans but also in lowering the acidogenicity of the biofilm. Thus, this technique could inhibit tooth demineralization. CLINICAL RELEVANCE: H2O2 photolysis can be applicable as a new dental caries treatment.

  17. Putative Mechanisms Underlying the Beneficial Effects of Polyphenols in Murine Models of Metabolic Disorders in Relation to Gut Microbiota Peer-reviewed

    Yoshimi Niwano, Hidetsugu Kohzaki, Midori Shirato, Shunichi Shishido, Keisuke Nakamura

    Current Issues in Molecular Biology　44　(3)　1353-1375　2022/03/18

    Publisher: MDPI AG

    DOI： 10.3390/cimb44030091 　

    eISSN： 1467-3045

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    The beneficial effects of polyphenols on metabolic disorders have been extensively reported. The interaction of these compounds with the gut microbiota has been the focus of recent studies. In this review, we explored the fundamental mechanisms underlying the beneficial effects of polyphenols in relation to the gut microbiota in murine models of metabolic disorders. We analyzed the effects of polyphenols on three murine models of metabolic disorders, namely, models of a high-fat diet (HFD)-induced metabolic disorder, dextran sulfate sodium (DSS)-induced colitis, and a metabolic disorder not associated with HFD or DSS. Regardless of the model, polyphenols ameliorated the effects of metabolic disorders by alleviating intestinal oxidative stress, improving inflammatory status, and improving intestinal barrier function, as well as by modulating gut microbiota, for example, by increasing the abundance of short-chain fatty acid-producing bacteria. Consequently, polyphenols reduce circulating lipopolysaccharide levels, thereby improving inflammatory status and alleviating oxidative imbalance at the lesion sites. In conclusion, polyphenols likely act by regulating intestinal functions, including the gut microbiota, and may be a safe and suitable therapeutic agent for various metabolic disorders.

  18. Anti-Osteoporotic Mechanisms of Polyphenols Elucidated Based on In Vivo Studies Using Ovariectomized Animals. International-journal Peer-reviewed

    Yoshimi Niwano, Hidetsugu Kohzaki, Midori Shirato, Shunichi Shishido, Keisuke Nakamura

    Antioxidants (Basel, Switzerland)　11　(2)　2022/01/23

    DOI： 10.3390/antiox11020217 　

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    Polyphenols are widely known for their antioxidant activity, i.e., they have the ability to suppress oxidative stress, and this behavior is mediated by the autoxidation of their phenolic hydroxyl groups. Postmenopausal osteoporosis is a common health problem that is associated with estrogen deficiency. Since oxidative stress is thought to play a key role in the onset and progression of osteoporosis, it is expected that polyphenols can serve as a safe and suitable treatment in this regard. Therefore, in this review, we aimed to elucidate the anti-osteoporotic mechanisms of polyphenols reported by in vivo studies involving the use of ovariectomized animals. We categorized the polyphenols as resveratrol, purified polyphenols other than resveratrol, or polyphenol-rich substances or extracts. Literature data indicated that resveratrol activates sirtuin 1, and thereafter, suppresses osteoclastogenic pathways, such as the receptor activator of the nuclear factor kappa B (RANK) ligand (RANKL) pathway, and promotes osteoblastogenic pathways, such as the wingless-related MMTV integration site pathway. Further, we noted that purified polyphenols and polyphenol-rich substances or extracts exert anti-inflammatory and/or antioxidative effects, which inhibit RANKL/RANK binding via the NF-κB pathway, resulting in the suppression of osteoclastogenesis. In conclusion, antioxidative and anti-inflammatory polyphenols, including resveratrol, can be safe and effective for the treatment of postmenopausal osteoporosis based on their ability to regulate the imbalance between bone formation and resorption.

  19. Resin Cement–Zirconia Bond Strengthening by Exposure to Low-Temperature Atmospheric Pressure Multi-Gas Plasma International-journal Peer-reviewed

    Nobuhiro Yoda, Yuri Abe, Yuma Suenaga, Yoshiki Matsudate, Tomohiro Hoshino, Takehiko Sugano, Keisuke Nakamura, Akitoshi Okino, Keiichi Sasaki

    Materials　15　(2)　2022/01/14

    DOI： 10.3390/ma15020631 　

    eISSN： 1996-1944

  20. Impact of adhesive primer and light-curing on polymerization kinetics of self-adhesive resin cement in association with free radical reaction. International-journal Peer-reviewed

    Akane Ozaki, Shunichi Shishido, Keisuke Nakamura, Akio Harada, Yusuke Katsuda, Taro Kanno, Hiroshi Egusa

    European journal of oral sciences　2021/10/21

    DOI： 10.1111/eos.12828 　

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    This study analyzed the impact of adhesive primer and light-curing on the polymerization kinetics of urethane dimethacrylate-based self-adhesive resin cement combined with free radical reaction. Specimens were prepared by mixing the cement paste with or without adhesive primer. Subsequently, specimens were light-cured or set without light-curing. The degree of conversion (DC), Vickers hardness (Hv), and free radical concentrations were repeatedly measured up to 168 h after the curing initiation. Irrespective of the curing procedures, DC, Hv, and free radical concentration rapidly increased during the initial 30 min of curing. The specimens cured with adhesive primer and/or light-curing generally showed higher values of DC, Hv, and radical concentration than those set by chemical curing alone, especially during the initial polymerization phase. Kinetic analysis using a linear mixed model revealed that the adhesive primer had a higher coefficient estimate than light-curing, indicating that the former had a higher impact on the polymerization. Additionally, the adhesive primer alleviated the Hv reduction caused by water and air during the initial polymerization phase, although light-curing hardly prevented the polymerization inhibition. Therefore, we suggest that application of adhesive primer is beneficial to achieve higher degree of conversion and better mechanical properties of self-adhesive resin cements by enhancing free radical reactions.

  21. Influence of crystalline phase transformation induced by airborne-particle abrasion and low-temperature degradation on mechanical properties of dental zirconia ceramics stabilized with over 5 mol% yttria. International-journal Peer-reviewed

    Keisuke Nakamura, Aria Tarkeshi, Alexander Niklasson, Shunichi Shishido, Per Svanborg, Sargon Barkarmo, Ulf Örtengren

    Journal of the mechanical behavior of biomedical materials　125　104890-104890　2021/10/13

    DOI： 10.1016/j.jmbbm.2021.104890 　

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    Monolithic dental prostheses fabricated from 5 mol% yttria-stabilized zirconia (5YZ) have been developed to improve the translucency of conventional 3 mol% yttria-stabilized zirconia. In this study, we aimed to evaluate the influence of airborne-particle abrasion (APA) and low-temperature degradation (LTD) on the mechanical properties of 5YZ in association with the crystalline phase transformation. In total, 120 disc-shaped specimens of two brands of 5YZ (Lava Esthetic and Katana UTML) were prepared. The specimens were divided into four groups (n = 15 for each group): (i) control, (ii) APA, (iii) LTD, and (iv) APA + LTD groups. APA was performed with 50 μm alumina particles, and LTD was induced by autoclaving at 134 °C for 50 h. The biaxial flexural strength of the specimens was assessed using a piston-on-three-ball test according to ISO 6872:2015, and Vickers hardness was determined using a microhardness tester. The crystalline phase was analyzed by the Rietveld refinement of X-ray diffraction patterns. APA significantly increased the flexural strength of the Lava Esthetic specimens, whereas LTD hardly affected the strength of both materials. APA and APA + LTD significantly increased the Vickers hardness of both materials. According to Rietveld analysis, the pseudocubic phase was predominant in both materials, i.e., 66 mass% and 81 mass% in the Lava Esthetic and Katana UTML specimens, respectively. APA induced the rhombohedral phase at approximately 37 mass% in both materials, while LTD induced the monoclinic phase at 2.8 mass% in the Lava Esthetic specimens and 0.9 mass% in the Katana UTML specimens. APA + LTD weakly affected the amount of the rhombohedral phase but slightly increased the amount of the monoclinic phase. These findings suggest that APA may improve the mechanical properties of 5YZ, particularly hardness, via the generation of the rhombohedral phase. In contrast, the influence of LTD on the mechanical and microstructural properties of 5YZ was limited.

  22. Gene transfection achieved by utilizing antibacterial calcium phosphate nanoparticles for enhanced regenerative therapy. International-journal Peer-reviewed

    Chen Xiang, Taichi Tenkumo, Toru Ogawa, Yoshiaki Kanda, Keisuke Nakamura, Midori Shirato, Viktoriya Sokolova, Matthias Epple, Yuya Kamano, Hiroshi Egusa, Keiichi Sasaki

    Acta biomaterialia　119　375-389　2021/01/01

    DOI： 10.1016/j.actbio.2020.11.003 　

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    Protamine-coated multi-shell calcium phosphate (CaP) was developed as a non-viral vector for tissue regeneration therapy. CaP nanoparticles loaded with different amounts of plasmid DNA encoding bone morphogenetic protein 2 (BMP-2) and insulin-like growth factor 1 (IGF-1) were used to treat MC3T3E1 cells, and the yield of the released BMP-2 or IGF-1 was measured using ELISA 3 days later. Collagen scaffolds containing CaP nanoparticles were implanted into rat cranial bone defects, and BMP-2 and IGF-1 yields, bone formation, and bone mineral density enhancement were evaluated 28 days after gene transfer. The antibacterial effects of CaP nanoparticles against Streptococcus mutans and Aggregatibacter actinomycetemcomitans increased with an increase in the protamine dose, while they were lower for Staphylococcus aureus and Porphyromonas gingivalis. In the combination treatment with BMP-2 and IGF-1, the concentration ratio of BMP-2 and IGF-1 is an important factor affecting bone formation activity. The calcification activity and OCN mRNA of MC3T3E1 cells subjected to a BMP-2:IGF-1 concentration ratio of 1:4 was higher at 14 days. During gene transfection treatment, BMP-2 and IGF-1 were released simultaneously after gene transfer; the loaded dose of the plasmid DNA encoding IGF-1 did not impact the BMP-2 or IGF-1 yield or new bone formation ratio in vitro and in vivo. In conclusion, two growth factor-releasing systems were developed using an antibacterial gene transfer vector, and the relationship between the loaded plasmid DNA dose and resultant growth factor yield was determined in vitro and in vivo.

  23. Effect of airborne-particle abrasion and polishing on novel translucent zirconias: Surface morphology, phase transformation and insights into bonding Peer-reviewed

    Sebastian Franco-Tabares, Dariusz Wardecki, Keisuke Nakamura, Sina Ardalani, Lars Hjalmarsson, Victoria Franke Stenport, Carina B Johansson

    Journal of Prosthodontic Research　Online ahead of print　2021

    Publisher: Japan Prosthodontic Society

    DOI： 10.2186/jpr.jpor_2019_524 　

    ISSN： 1883-1958 1883-9207

    eISSN： 1883-9207

  24. Reactions of dental pulp to hydrogen peroxide photolysis-based antimicrobial chemotherapy under ultraviolet-A irradiation in rats Peer-reviewed

    Keisuke Nakamura, Midori Shirato, Shunichi Shishido, Yoshimi Niwano, Taro Kanno, Keiichi Sasaki, Peter Lingström, Ulf Örtengren

    Journal of Photochemistry and Photobiology B: Biology　212　112042-112042　2020/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.jphotobiol.2020.112042 　

    ISSN： 1011-1344

  25. Mechanical and microstructural properties of ultra-translucent dental zirconia ceramic stabilized with 5 mol% yttria International-journal Peer-reviewed

    Akio Harada, Shunichi Shishido, Sargon Barkarmo, Ryoichi Inagaki, Taro Kanno, Ulf Örtengren, Hiroshi Egusa, Keisuke Nakamura

    Journal of the Mechanical Behavior of Biomedical Materials　111　103974-103974　2020/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.jmbbm.2020.103974 　

    ISSN： 1751-6161

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    Monolithic dental prostheses fabricated from 3 mol.% yttria-stabilized zirconia (3YZ) are becoming increasingly popular. Recently, 5 mol.% yttria-stabilized zirconia (5YZ) which significantly improves the translucency of 3YZ has been prepared. However, its mechanical and microstructural properties, especially those affected by low-temperature degradation (LTD), have not been fully elucidated yet. The objective of the present study was to establish the relationship between the flexural strength of 5YZ with or without autoclave-induced LTD and its microstructural properties. For this purpose, a total of 320 bar-shaped specimens were cut from 5YZ and 3YZ blocks, and half of the specimens in each group were autoclaved at 134 °C for 50 h. Their flexural strengths were determined by conducting three-point bending tests, and the obtained results were analyzed by the Weibull statistical method. Grain sizes and crystalline structures of the specimens were analyzed by scanning electron microscopy (SEM) and X-ray diffraction, respectively. Additionally, the LTD-induced phase transformation was examined by Raman microscopy and cross-sectional surface analysis. The characteristic strengths of 5YZ and 3YZ were approximately 620 and 950 MPa, respectively, and 5YZ was found to be more resistant to LTD in terms of phase transformation than 3YZ. However, a low amount of the monoclinic phase was detected even in 5YZ after 50 h of autoclaving, which significantly decreased its flexural strength and reliability. The results of SEM analysis revealed that 5YZ was composed of two distinct regions: a dominant matrix with large grains (median size: 0.8 μm) and scattered areas with small grains (median size: 0.4 μm). Phase transformation analysis and fractography data indicated that the small-grain region was strongly affected by LTD and likely represented a fracture origin. The described properties should be considered during the clinical application of monolithic 5YZ dental prostheses.

  26. Proanthocyanidin-rich grape seed extract improves bone loss, bone healing, and implant osseointegration in ovariectomized animals Peer-reviewed

    Taichi Tenkumo, Alkebaier Aobulikasimu, Yoshinori Asou, Midori Shirato, Shunichi Shishido, Taro Kanno, Yoshimi Niwano, Keiichi Sasaki, Keisuke Nakamura

    Scientific Reports　10　8812-8812　2020/06

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-020-65403-4 　

    eISSN： 2045-2322

  27. Bactericidal activity and recovery effect of hydroxyl radicals generated by ultraviolet irradiation and silver ion application on an infected titanium surface International-journal Peer-reviewed

    Taichi Tenkumo, Kirika Ishiyama, Oleg Prymak, Keisuke Nakamura, Midori Shirato, Toru Ogawa, Makiko Miyashita, Masatoshi Takahashi, Matthias Epple, Taro Kanno, Keiichi Sasaki

    Scientific Reports　10　(1)　8553-8553　2020/05

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-020-65411-4 　

    eISSN： 2045-2322

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    This study investigated the bactericidal effect, the underlying mechanisms of treatment, and recovery of biocompatibility of the infected titanium surface using a combination treatment of silver ion application and ultraviolet-A (UV-A) light irradiation. Streptococcus mutans and Aggregatibacter actinomycetemcomitans were used in suspension and as a biofilm on a titanium surface to test for the bactericidal effect. The bactericidal effect of the combination treatment was significantly higher than that of silver ion application or UV-A light irradiation alone. The bactericidal effect of the combination treatment was attributable to hydroxyl radicals, which generated from the bacterial cell wall and whose yield increased with the silver concentration. To assess the biocompatibility, proliferation and calcification of MC3T3E1 cells were evaluated on the treated titanium surface. The treated titanium screws were implanted into rat tibias and the removal torques were measured 28 days post-surgery. The titanium surface that underwent the combination treatment exhibited recovery of biocompatibility by allowing cellular proliferation or calcification at levels observed in the non-infected titanium surfaces. The removal torque 28 days after surgery was also comparable to the control values. This approach is a novel treatment option for peri-implantitis.

  28. Oral mucosal irritation potential of antimicrobial chemotherapy involving hydrogen peroxide photolysis with high-power laser irradiation for the treatment of periodontitis. International-journal Peer-reviewed

    Midori Shirato, Keisuke Nakamura, Taichi Tenkumo, Yuki Kano, Kirika Ishiyama, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano, Hiroshi Matsuura

    Journal of photochemistry and photobiology. B, Biology　201　111633-111633　2019/12

    DOI： 10.1016/j.jphotobiol.2019.111633 　

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    In the present study, we assessed the oral mucosal irritation potential of antimicrobial chemotherapy involving hydrogen peroxide (H2O2) photolysis with a 405-nm laser device at an output power of ≥100 mW in hamsters. Twenty-four cheek pouches from 12 male Syrian hamsters received 7-min treatment with pure water (PW), 3% H2O2, laser irradiation of PW at 100 mW, laser irradiation of 3% H2O2 at 100 mW, laser irradiation of PW at 200 mW, or laser irradiation of 3% H2O2 at 200 mW (n = 4 each). The diameter of the irradiation area was set at 3 mm; accordingly, the calculated irradiances (optical power densities) of the 100- and 200-mW laser lights were approximately 1400 and 2800 mW/cm2, respectively. In addition, 12 cheek pouches from six animals received laser irradiation of 3% H2O2 at 100 mW for 1, 3, or 5 min (n = 4 each). Each treatment was repeated three times at 1-h intervals. Macroscopic and histological changes were evaluated 24 h after the last treatment. In addition, in vitro bactericidal activity of the treatment against periodontal pathogens was evaluated. We found that 405-nm laser irradiation of 3% H2O2 caused moderate to severe oral mucosal irritation when performed at powers of 100 and 200 mW for ≥3 min, while the same treatment performed at 100 mW for 1 min resulted in mild irritation. Moreover, 1-min H2O2 photolysis at 100 mW caused a >4-log decrease in viable bacterial counts. These findings suggest that 1-min H2O2 photolysis, which can effectively kill periodontal pathogens, may be acceptable when a 405-nm laser device is used at 100 mW. However, use of the laser at a lower power would be preferable for the prevention of unnecessary oral mucosal irritation.

  29. Hydroxyl radicals generated by hydrogen peroxide photolysis recondition biofilm-contaminated titanium surfaces for subsequent osteoblastic cell proliferation. International-journal Peer-reviewed

    Keisuke Nakamura, Midori Shirato, Taichi Tenkumo, Taro Kanno, Anna Westerlund, Ulf Örtengren, Keiichi Sasaki, Yoshimi Niwano

    Scientific reports　9　(1)　4688-4688　2019/03/18

    DOI： 10.1038/s41598-019-41126-z 　

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    Titanium dental implants have been successfully used for decades; however, some implants are affected by peri-implantitis due to bacterial infection, resulting in loss of supporting bone. This study aimed to evaluate the effect of an antimicrobial chemotherapy employing H2O2 photolysis-developed to treat peri-implantitis-on biofilm-contaminated titanium surfaces in association with osteoblastic cell proliferation on the treated surface. Titanium discs were sandblasted and acid-etched, followed by contamination with a three-species biofilm composed of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mitis. This biofilm model was used as a simplified model of clinical peri-implantitis biofilm. The discs were subjected to ultrasound scaling, followed by H2O2 photolysis, wherein 365-nm LED irradiation of the disc immersed in 3% H2O2 was performed for 5 min. We analysed proliferation of mouse osteoblastic cells (MC3T3-E1) cultured on the treated discs. Compared with intact discs, biofilm contamination lowered cell proliferation on the specimen surface, whereas H2O2 photolysis recovered cell proliferation. Thus, H2O2 photolysis can recover the degraded biocompatibility of biofilm-contaminated titanium surfaces and can potentially be utilised for peri-implantitis treatment. However, to verify the findings of this study in relation to clinical settings, assessment using a more clinically relevant multi-species biofilm model is necessary.

  30. Critical considerations on load-to-failure test for monolithic zirconia molar crowns. International-journal Peer-reviewed

    Keisuke Nakamura, Shuhei Ankyu, Fritjof Nilsson, Taro Kanno, Yoshimi Niwano, Per Vult von Steyern, Ulf Örtengren

    Journal of the mechanical behavior of biomedical materials　87　180-189　2018/11

    DOI： 10.1016/j.jmbbm.2018.07.034 　

    ISSN： 1751-6161

  31. Prolonged release of bone morphogenetic protein-2 in vivo by gene transfection with DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds Peer-reviewed

    Taichi Tenkumo, Juan Ramón Vanegas Sáenz, Keisuke Nakamura, Yoshinaka Shimizu, Viktoriya Sokolova, Matthias Epple, Yuya Kamano, Hiroshi Egusa, Tsutomu Sugaya, Keiichi Sasaki

    Materials Science and Engineering C　92　172-183　2018/11/01

    Publisher: Elsevier Ltd

    DOI： 10.1016/j.msec.2018.06.047 　

    ISSN： 0928-4931

  32. Influence of low-temperature degradation on the wear characteristics of zirconia against polymer-infiltrated ceramic-network material Peer-reviewed

    Zhichao Hao, Yuanyuan Ma, Wenjia Liu, Yukun Meng, Keisuke Nakamura, Jiefei Shen, Hang Wang

    Journal of Prosthetic Dentistry　120　(4)　596-602　2018/10

    Publisher: Mosby Inc.

    DOI： 10.1016/j.prosdent.2017.12.014 　

    ISSN： 0022-3913

  33. Potential adverse effects of antimicrobial chemotherapy based on ultraviolet-A irradiation of polyphenols against the oral mucosa in hamsters and wounded skin in rats. International-journal Peer-reviewed

    Keisuke Nakamura, Taichi Tenkumo, Takayuki Mokudai, Midori Shirato, Kirika Ishiyama, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    Journal of photochemistry and photobiology. B, Biology　187　96-105　2018/10

    DOI： 10.1016/j.jphotobiol.2018.08.006 　

    ISSN： 1011-1344

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    Antimicrobial chemotherapy based on ultraviolet-A (UVA) irradiation of polyphenol solution has been proposed as an adjunctive treatment for dental caries. However, the safety of this treatment has not been thoroughly evaluated. Therefore, the aim of the present study was to assess the influence of this treatment on the oral mucosa in hamsters and wounded skin in rats. An oral mucosal irritation test was performed in hamsters. The cheek pouch was everted and treated with UVA irradiation (wavelength: 365 nm) of pure water, 1 mg/mL of caffeic acid, or 1 mg/mL of grape seed extract using a light-emitting diode at an irradiance of 275 mW/cm2. Each treatment was performed for 2 or 5 min and repeated three times. Macroscopic and histological evaluations were performed 24 h after the last treatment. We also examined the effects of the treatment on the healing process of skin wounds in rats. Full-thickness skin wounds created on the back of the rats were treated for 2 min as described above, but only once. The wound area was then assessed daily for 9 days. The results demonstrated that the treatment induced oral mucosal irritation depending on the irradiation time; however, it did not influence the wound healing process. The oral mucosal irritation potential of three treatment sessions performed for 2 and 5 min was minimal and mild to moderate, respectively, according to histological analysis. These findings suggest that the duration of treatment based on UVA irradiation of polyphenols in the oral cavity should be as short as possible, considering the clinical efficacy of the antimicrobial effects and the irritation potential.

  34. Influence of microscale expansion and contraction caused by thermal and mechanical fatigue on retentive strength of CAD/CAM-generated resin-based composite crowns. International-journal Peer-reviewed

    Shuhei Ankyu, Keisuke Nakamura, Akio Harada, Ryoichi Inagaki, Yusuke Katsuda, Taro Kanno, Yoshimi Niwano, Ulf Örtengren, Hiroshi Egusa

    Journal of the mechanical behavior of biomedical materials　86　89-97　2018/10

    DOI： 10.1016/j.jmbbm.2018.06.025 　

    ISSN： 1751-6161

  35. A novel pathway for the photooxidation of catechin in relation to its prooxidative activity. International-journal Peer-reviewed

    Shunichi Shishido, Rei Miyano, Takuji Nakashima, Hirotaka Matsuo, Masato Iwatsuki, Keisuke Nakamura, Taro Kanno, Hiroshi Egusa, Yoshimi Niwano

    Scientific reports　8　(1)　12888-12888　2018/08/27

    DOI： 10.1038/s41598-018-31195-x 　

  36. 遺伝子導入剤を含有したナノハイドロキシアパタイトーコラーゲンscaffoldの開発

    天雲 太一, 中村 圭祐, 佐々木 啓一, 菅野 太郎

    日本歯周病学会会誌　60　(春季特別)　130-130　2018/05

    Publisher: (NPO)日本歯周病学会

    ISSN： 0385-0110

    eISSN： 1880-408X

  37. Surface properties of dental zirconia ceramics affected by ultrasonic scaling and low-temperature degradation. International-journal Peer-reviewed

    Kosuke Nakazawa, Keisuke Nakamura, Akio Harada, Midori Shirato, Ryoichi Inagaki, Ulf Örtengren, Taro Kanno, Yoshimi Niwano, Hiroshi Egusa

    PloS one　13　(9)　e0203849　2018

    DOI： 10.1371/journal.pone.0203849 　

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    Zirconia (3Y-TZP) dental prostheses are widely used in clinical dentistry. However, the effect of ultrasonic scaling performed as a part of professional tooth cleaning on 3Y-TZP dental prostheses, especially in conjunction with low-temperature degradation (LTD), has not been fully investigated. The present study aimed to evaluate the influence of ultrasonic scaling and LTD on the surface properties of 3Y-TZP in relation to bacterial adhesion on the treated surface. 3Y-TZP specimens (4 × 4 × 2 mm) were polished and then subjected to autoclaving at 134°C for 100 h to induce LTD, followed by 10 rounds of ultrasonic scaling using a steel scaler tip for 1 min each. Surface roughness, crystalline structure, wettability, and hardness were analyzed by optical interferometry, X-ray diffraction analysis, contact angle measurement, and nano-indentation technique, respectively. Subsequently, bacterial adhesion onto the treated 3Y-TZP surface was evaluated using Streptococcus mitis and S. oralis. The results demonstrated that the combination of ultrasonic scaling and LTD significantly increased the Sa value (surface roughness parameter) of the polished 3Y-TZP surface from 1.6 nm to 117 nm. LTD affected the crystalline structure, causing phase transformation from the tetragonal to the monoclinic phase, and decreased both the contact angle and surface hardness. However, bacterial adhesion was not influenced by these changes in surface properties. The present study suggests that ultrasonic scaling may be acceptable for debridement of 3Y-TZP dental prostheses because it did not facilitate bacterial adhesion even in the combination with LTD, although it did cause slight roughening of the surface.

  38. Oral mucosal irritation study in hamster to evaluate a therapeutic apparatus using hydrogen peroxide photolysis for periodontitis treatment Peer-reviewed

    Yoshimi Niwano, Kazunori Konno, Takeshi Matayoshi, Keisuke Nakamura, Taro Kanno, Keiichi Sasaki

    REGULATORY TOXICOLOGY AND PHARMACOLOGY　90　206-213　2017/11

    DOI： 10.1016/j.yrtph.2017.09.019 　

    ISSN： 0273-2300

    eISSN： 1096-0295

  39. Adjunctive antimicrobial chemotherapy based on hydrogen peroxide photolysis for non-surgical treatment of moderate to severe periodontitis: a randomized controlled trial Peer-reviewed

    Taro Kanno, Keisuke Nakamura, Kirika Ishiyama, Yasutomo Yamada, Midori Shirato, Yoshimi Niwano, Chie Kayaba, Koji Ikeda, Airi Takagi, Takuhiro Yamaguchi, Keiichi Sasaki

    SCIENTIFIC REPORTS　7　12247　2017/09

    DOI： 10.1038/s41598-017-12514-0 　

    ISSN： 2045-2322

  40. Time-kill kinetic analysis of antimicrobial chemotherapy based on hydrogen peroxide photolysis against Streptococcus mutans biofilm Peer-reviewed

    Midori Shirato, Keisuke Nakamura, Taro Kanno, Peter Lingstrom, Yoshimi Niwano, Ulf Ortengren

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY　173　434-440　2017/08

    DOI： 10.1016/j.jphotobiol.2017.06.023 　

    ISSN： 1011-1344

  41. Photo-irradiated caffeic acid exhibits antimicrobial activity against Streptococcus mutans biofilms via hydroxyl radical formation Peer-reviewed

    Keisuke Nakamura, Midori Shirato, Taro Kanno, Peter Lingstrom, Ulf Ortengren, Yoshimi Niwano

    SCIENTIFIC REPORTS　7　6353　2017/07

    DOI： 10.1038/s41598-017-07007-z 　

    ISSN： 2045-2322

  42. Efficacy and safety of a therapeutic apparatus using hydrogen peroxide photolysis to treat dental and periodontal infectious diseases Peer-reviewed

    Hirohisa Sato, Yoshimi Niwano, Keisuke Nakamura, Takayuki Mokudai, Hiroyo Ikai, Taro Kanno, Hiroshi Egusa

    JOURNAL OF TOXICOLOGICAL SCIENCES　41　(6)　793-799　2016/12

    DOI： 10.2131/jts.41.793 　

    ISSN： 0388-1350

    eISSN： 1880-3989

  43. Antimicrobial activity of hydroxyl radicals generated by hydrogen peroxide photolysis against Streptococcus mutans biofilm Peer-reviewed

    Keisuke Nakamura, Midori Shirato, Taro Kanno, Ulf Ortengren, Peter Lingstrom, Yoshimi Niwano

    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS　48　(4)　373-380　2016/10

    DOI： 10.1016/j.ijantimicag.2016.06.007 　

    ISSN： 0924-8579

    eISSN： 1872-7913

  44. Bactericidal Action of Photodynamic Antimicrobial Chemotherapy (PACT) with Photosensitizers Used as Plaque-Disclosing Agents against Experimental Biofilm Peer-reviewed

    Kirika Ishiyama, Keisuke Nakamura, Taro Kanno, Yoshimi Niwano

    BIOCONTROL SCIENCE　21　(3)　187-191　2016/09

    DOI： 10.4265/bio.21.187 　

    ISSN： 1342-4815

    eISSN： 1884-0205

  45. Fatigue analysis of computer-aided design/computer-aided manufacturing resin-based composite vs. lithium disilicate glass-ceramic Peer-reviewed

    Shuhei Ankyu, Keisuke Nakamura, Akio Harada, Guang Hong, Taro Kanno, Yoshimi Niwano, Ulf Ortengren, Hiroshi Egusa

    EUROPEAN JOURNAL OF ORAL SCIENCES　124　(4)　387-395　2016/08

    DOI： 10.1111/eos.12278 　

    ISSN： 0909-8836

    eISSN： 1600-0722

  46. Effect of low-temperature degradation on the mechanical and microstructural properties of tooth-colored 3Y-TZP ceramics Peer-reviewed

    K. Nakamura, A. Harada, M. Ono, H. Shibasaki, T. Kanno, Y. Niwano, E. Adolfsson, R. Milleding, U. Ortengren

    JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS　53　301-311　2016/01

    DOI： 10.1016/j.jmbbm.2015.08.031 　

    ISSN： 1751-6161

    eISSN： 1878-0180

  47. 活性酸素・フリーラジカルを応用した殺菌技術 Peer-reviewed

    中村 圭祐

    日本防菌防黴学会誌　44　191-197　2016

  48. Effect of cements on fracture resistance of monolithic zirconia crowns. Peer-reviewed

    Nakamura K, Mouhat M, Nergård JM, Lægreid SJ, Kanno T, Milleding P, Örtengren U

    Acta Biomater Odont Scand　2　(1)　12-19　2016/01

    DOI： 10.3109/23337931.2015.1129908 　

  49. Cutting efficiency of diamond burs operated with electric high-speed dental handpiece on zirconia Peer-reviewed

    Keisuke Nakamura, Yusuke Katsuda, Shuhei Ankyu, Akio Harada, Taichi Tenkumo, Taro Kanno, Yoshimi Niwano, Hiroshi Egusa, Percy Milleding, Ulf Ortengren

    EUROPEAN JOURNAL OF ORAL SCIENCES　123　(5)　375-380　2015/10

    DOI： 10.1111/eos.12211 　

    ISSN： 0909-8836

    eISSN： 1600-0722

  50. Cytoprotective Effects of Grape Seed Extract on Human Gingival Fibroblasts in Relation to Its Antioxidant Potential (vol 10, e0134704, 2015)

    Yusuke Katsuda, Yoshimi Niwano, Takuji Nakashima, Takayuki Mokudai, Keisuke Nakamura, Satomi Oizumi, Taro Kanno, Hiroyasu Kanetaka, Hiroshi Egusa

    PLOS ONE　10　(9)　2015/09

    DOI： 10.1371/journal.pone.0138394 　

    ISSN： 1932-6203

  51. Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria Peer-reviewed

    Keisuke Nakamura, Kirika Ishiyama, Hong Sheng, Hiroyo Ikai, Taro Kanno, Yoshimi Niwano

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY　63　(35)　7707-7713　2015/09

    DOI： 10.1021/jf5058588 　

    ISSN： 0021-8561

    eISSN： 1520-5118

  52. Cytoprotective Effects of Grape Seed Extract on Human Gingival Fibroblasts in Relation to Its Antioxidant Potential Peer-reviewed

    Yusuke Katsuda, Yoshimi Niwano, Takuji Nakashima, Takayuki Mokudai, Keisuke Nakamura, Satomi Oizumi, Taro Kanno, Hiroyasu Kanetaka, Hiroshi Egusa

    PLOS ONE　10　(8)　e0134704　2015/08

    DOI： 10.1371/journal.pone.0134704 　

    ISSN： 1932-6203

  53. Cytoprotective Effects of Grape Seed Extract on Human Gingival Fibroblasts in Relation to Its Antioxidant Potential Peer-reviewed

    Yusuke Katsuda, Yoshimi Niwano, Takuji Nakashima, Takayuki Mokudai, Keisuke Nakamura, Satomi Oizumi, Taro Kanno, Hiroyasu Kanetaka, Hiroshi Egusa

    PLOS ONE　10　(8)　e0138394　2015/08

    DOI： 10.1371/journal.pone.0134704 　

    ISSN： 1932-6203

  54. Bactericidal Effect of Photolysis of H2O2 in Combination with Sonolysis of Water via Hydroxyl Radical Generation Peer-reviewed

    Hong Sheng, Keisuke Nakamura, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    PLOS ONE　10　(7)　e0132445　2015/07

    DOI： 10.1371/journal.pone.0132445 　

    ISSN： 1932-6203

  55. The influence of low-temperature degradation and cyclic loading on the fracture resistance of monolithic zirconia molar crowns

    K. Nakamura, A. Harada, T. Kanno, R. Inagaki, Y. Niwano, P. Milleding, U. Ortengren

    JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS　47　49-56　2015/07

    DOI： 10.1016/j.jmbbm.2015.03.007 　

    ISSN： 1751-6161

    eISSN： 1878-0180

  56. Fracture resistance of computer-aided design/computer-aided manufacturing generated composite resin-based molar crowns Peer-reviewed

    Akio Harada, Keisuke Nakamura, Taro Kanno, Ryoichi Inagaki, Ulf Ortengren, Yoshimi Niwano, Keiichi Sasaki, Hiroshi Egusa

    EUROPEAN JOURNAL OF ORAL SCIENCES　123　(2)　122-129　2015/04

    DOI： 10.1111/eos.12173 　

    ISSN： 0909-8836

    eISSN： 1600-0722

  57. Synergistic interaction between wavelength of light and concentration of H2O2 in bactericidal activity of photolysis of H2O2 Peer-reviewed

    Toshihide Toki, Keisuke Nakamura, Michiko Kurauchi, Taro Kanno, Yusuke Katsuda, Hiroyo Ikai, Eisei Hayashi, Hiroshi Egusa, Keiichi Sasaki, Yoshimi Niwano

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING　119　(3)　358-362　2015/03

    DOI： 10.1016/j.jbiosc.2014.08.015 　

    ISSN： 1389-1723

    eISSN： 1347-4421

  58. Mechanical and microstructural properties of monolithic zirconia: Crown fracture resistance and impact of low-temperature degradation Peer-reviewed

    Keisuke Nakamura

    University of Gothenburg　2015

  59. Fracture resistance of monolithic zirconia molar crowns with reduced thickness Peer-reviewed

    Keisuke Nakamura, Akio Harada, Ryoichi Inagaki, Taro Kanno, Yoshimi Niwano, Percy Milleding, Ulf Ortengren

    ACTA ODONTOLOGICA SCANDINAVICA　73　(8)　602-608　2015

    DOI： 10.3109/00016357.2015.1007479 　

    ISSN： 0001-6357

    eISSN： 1502-3850

  60. Acceleration of Proliferative Response of Mouse Fibroblasts by Short-Time Pretreatment with Polyphenols Peer-reviewed

    Makoto Tsuruya, Yoshimi Niwano, Keisuke Nakamura, Taro Kanno, Takuji Nakashima, Hiroshi Egusa, Keiichi Sasaki

    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY　174　(6)　2223-2235　2014/11

    DOI： 10.1007/s12010-014-1124-7 　

    ISSN： 0273-2289

    eISSN： 1559-0291

  61. Cytoprotective Effect of Short-Term Pretreatment with Proanthocyanidin on Human Gingival Fibroblasts Exposed to Harsh Environmental Conditions Peer-reviewed

    Michiko Kurauchi, Yoshimi Niwano, Midori Shirato, Taro Kanno, Keisuke Nakamura, Hiroshi Egusa, Keiichi Sasaki

    PLOS ONE　9　(11)　e113403　2014/11

    DOI： 10.1371/journal.pone.0113403 　

    ISSN： 1932-6203

  62. Postantibiotic effect of disinfection treatment by photolysis of hydrogen peroxide Peer-reviewed

    Yu Odashima, Keisuke Nakamura, Hiroyo Ikai, Taro Kanno, Luiz Meirelles, Keiichi Sasaki, Yoshimi Niwano

    JOURNAL OF CHEMOTHERAPY　26　(2)　92-100　2014/04

    DOI： 10.1179/1973947813Y.0000000114 　

    ISSN： 1120-009X

    eISSN： 1973-9478

  63. Synergistic Effect of Proanthocyanidin on the Bactericidal Action of the Photolysis of H2O2 Peer-reviewed

    Hiroyo Ikai, Keisuke Nakamura, Taro Kanno, Midori Shirato, Luiz Meirelles, Keiichi Sasaki, Yoshimi Niwano

    BIOCONTROL SCIENCE　18　(3)　137-141　2013/09

    DOI： 10.4265/bio.18.137 　

    ISSN： 1342-4815

    eISSN： 1884-0205

  64. Influence of a new denture cleaning technique based on photolysis of H2O2 on the mechanical properties and color change of acrylic denture base resin Peer-reviewed

    Tatsuro Nakahara, Akio Harada, Yasutomo Yamada, Yu Odashima, Keisuke Nakamura, Ryoichi Inagaki, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    DENTAL MATERIALS JOURNAL　32　(4)　529-536　2013/08

    DOI： 10.4012/dmj.2013-047 　

    ISSN： 0287-4547

  65. Photo-Irradiation of Proanthocyanidin as a New Disinfection Technique via Reactive Oxygen Species Formation Peer-reviewed

    Keisuke Nakamura, Midori Shirato, Hiroyo Ikai, Taro Kanno, Keiichi Sasaki, Masahiro Kohno, Yoshimi Niwano

    PLOS ONE　8　(3)　e60053　2013/03

    DOI： 10.1371/journal.pone.0060053 　

    ISSN： 1932-6203

  66. In vitro bactericidal activity of photo-irradiated oxydol products via hydroxyl radical generation. Peer-reviewed

    Akira Oyamada, Hiroyo Ikai, Keisuke Nakamura, Eisei Hayashi, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    Biocontrol science　18　(2)　83-8　2013

    DOI： 10.4265/bio.18.83 　

    ISSN： 1342-4815

    eISSN： 1884-0205

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    The oxidative power of hydroxyl radicals has been applied to disinfection systems for the purpose of oral hygiene by utilizing blue light-induced photolysis of hydrogen peroxide (H₂O₂) in our laboratory. In the present study, the bactericidal potential of blue light-irradiated oxydol products via hydroxyl radical generation was compared with that of 3% (w/v) H₂O₂. Eleven commercially available oxydol products were used in the present study. Even though a few of the products that contained ethanol, a hydroxyl radical scavenger, as an additive showed slightly lower hydroxyl radical yield as compared with 3% (w/v) H₂O₂, the blue-light irradiation of each oxydol product for 3 min showed similar or superior bactericidal activity against Staphylococcus aureus to that of 3% (w/v) H₂O₂. The results strongly suggest that any of the oxydol products tested in the present study can be used as a source of hydroxyl radicals for the disinfection technique developed in our laboratory.

  67. In vitro evaluation of the risk of inducing bacterial resistance to disinfection treatment with photolysis of hydrogen peroxide. International-journal Peer-reviewed

    Hiroyo Ikai, Yu Odashima, Taro Kanno, Keisuke Nakamura, Midori Shirato, Keiichi Sasaki, Yoshimi Niwano

    PloS one　8　(11)　e81316　2013

    DOI： 10.1371/journal.pone.0081316 　

    More details Close

    The purpose of the present study was to evaluate the risk of inducing bacterial resistance to disinfection treatment with photolysis of H2O2 and comparing this with existing antibacterial agents. We tested seven antibacterial agents, including amoxicillin, cefepime hydrochloride, erythromycin, ofloxacin, clindamycin hydrochloride, ciprofloxacin hydrochloride, and minocycline hydrochloride, as positive controls for validation of the assay protocol. For all of the agents tested, at least one of the four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Streptococcus salivarius) was resistant to these agents by repeated exposure to subinhibitory concentrations of the agents up to 10 times. In contrast, antibacterial activity against any of the bacterial species tested (S. aureus, E. faecalis, E. coli, S. salivarius, Pseudomonas aeruginosa, Streptococcus mutans, and Aggregatibacter actinomycetemcomitans) was not affected by repeated exposure to the disinfection treatment up to 40 times. This finding suggested that the risk of inducing bacterial resistance by disinfection treatment was low. The active ingredient of this disinfection treatment is hydroxyl radicals generated by photolysis of H2O2. Therefore, hydroxyl radicals interact with several cell structures and different metabolic pathways in microbial cells, probably resulting in a lack of development of bacterial resistance. In conclusion, disinfection treatment with photolysis of H2O2 appears to be a potential alternative for existing antimicrobial agents in terms of a low risk of inducing bacterial resistance.

  68. Corrosive effect of disinfection solution containing hydroxyl radicals generated by photolysis of H2O2 on dental metals Peer-reviewed

    Keisuke Nakamura, Yasutomo Yamada, Yukyo Takada, Takayuki Mokudai, Hiroyo Ikai, Ryoichi Inagaki, Taro Kanno, Keiichi Sasaki, Masahiro Kohno, Yoshimi Niwano

    DENTAL MATERIALS JOURNAL　31　(6)　941-946　2012/11

    DOI： 10.4012/dmj.2012-098 　

    ISSN： 0287-4547

  69. Topical treatment of oral cavity and wounded skin with a new disinfection system utilizing photolysis of hydrogen peroxide in rats (vol 37, pg 329, 2012)

    Yasutomo Yamada, Takayuki Mokudai, Keisuke Nakamura, Eisei Hayashi, Yoshiko Kawana, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    JOURNAL OF TOXICOLOGICAL SCIENCES　37　(5)　1091-1091　2012/10

    ISSN： 0388-1350

    eISSN： 1880-3989

  70. Erratum to Topical treatment of oral cavity and wounded skin with a new disinfection system utilizing photolysis of hydrogen peroxide in rats (J. Toxicol. Sci., (2012), 37, (329-335))

    Yamada Y, Mokudai T, Nakamura K, Hayashi E, Kawana Y, Kanno T, Sasaki K, Niwano Y

    J Toxicol Sci　37　1091　2012/10

    ISSN： 1880-3989

  71. Bactericidal Action of Photoirradiated Gallic Acid via Reactive Oxygen Species Formation Peer-reviewed

    Keisuke Nakamura, Yasutomo Yamada, Hiroyo Ikai, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY　60　(40)　10048-10054　2012/10

    DOI： 10.1021/jf303177p 　

    ISSN： 0021-8561

  72. In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide Peer-reviewed

    Eisei Hayashi, Takayuki Mokudai, Yasutomo Yamada, Keisuke Nakamura, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING　114　(2)　193-197　2012/08

    DOI： 10.1016/j.jbiosc.2012.03.020 　

    ISSN： 1389-1723

  73. Novel Denture-Cleaning System Based on Hydroxyl Radical Disinfection Peer-reviewed

    Taro Kanno, Keisuke Nakamura, Hiroyo Ikai, Eisei Hayashi, Midori Shirato, Takayuki Mokudai, Atsuo Iwasawa, Yoshimi Niwano, Masahiro Kohno, Keiichi Sasaki

    INTERNATIONAL JOURNAL OF PROSTHODONTICS　25　(4)　376-380　2012/07

    ISSN： 0893-2174

  74. Literature review of the role of hydroxyl radicals in chemically-induced mutagenicity and carcinogenicity for the risk assessment of a disinfection system utilizing photolysis of hydrogen peroxide Peer-reviewed

    Taro Kanno, Keisuke Nakamura, Hiroyo Ikai, Katsushi Kikuchi, Keiichi Sasaki, Yoshimi Niwano

    JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION　51　(1)　9-14　2012/07

    DOI： 10.3164/jcbn.11-105 　

    ISSN： 0912-0009

    eISSN： 1880-5086

  75. Determination of reactive oxygen species generated by phorbol 12-myristate 13-acetate-stimulated oral polymorphonuclear cells from healthy human volunteers without any dental problems Peer-reviewed

    Noriko Yaekashiwa, Emiko Sato, Keisuke Nakamura, Atsuo Iwasawa, Akihito Kudo, Taro Kanno, Masahiro Kohno, Yoshimi Niwano

    ARCHIVES OF ORAL BIOLOGY　57　(6)　636-641　2012/06

    DOI： 10.1016/j.archoralbio.2011.10.020 　

    ISSN： 0003-9969

    eISSN： 1879-1506

  76. Influence of grain size and veneer firing process on the flexural strength of zirconia ceramics Peer-reviewed

    Keisuke Nakamura, Erik Adolfsson, Percy Milleding, Taro Kanno, Ulf Ortengren

    EUROPEAN JOURNAL OF ORAL SCIENCES　120　(3)　249-254　2012/06

    DOI： 10.1111/j.1600-0722.2012.00958.x 　

    ISSN： 0909-8836

  77. Bactericidal Action of Photogenerated Singlet Oxygen from Photosensitizers Used in Plaque Disclosing Agents Peer-reviewed

    Kirika Ishiyama, Keisuke Nakamura, Hiroyo Ikai, Taro Kanno, Masahiro Kohno, Keiichi Sasaki, Yoshimi Niwano

    PLOS ONE　7　(5)　e37871　2012/05

    DOI： 10.1371/journal.pone.0037871 　

    ISSN： 1932-6203

  78. Topical treatment of oral cavity and wounded skin with a new disinfection system utilizing photolysis of hydrogen peroxide in rats Peer-reviewed

    Yasutomo Yamada, Takayuki Mokudai, Keisuke Nakamura, Eisei Hayashi, Yoshiko Kawana, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    JOURNAL OF TOXICOLOGICAL SCIENCES　37　(2)　329-335　2012/04

    DOI： 10.2131/jts.37.329 　

    ISSN： 0388-1350

    eISSN： 1880-3989

  79. Microbial resistance in relation to catalase activity to oxidative stress induced by photolysis of hydrogen peroxide Peer-reviewed

    Keisuke Nakamura, Taro Kanno, Takayuki Mokudai, Atsuo Iwasawa, Yoshimi Niwano, Masahiro Kohno

    MICROBIOLOGY AND IMMUNOLOGY　56　(1)　48-55　2012/01

    DOI： 10.1111/j.1348-0421.2011.00400.x 　

    ISSN： 0385-5600

  80. Synergistic Effect of Thermal Energy on Bactericidal Action of Photolysis of H2O2 in Relation to Acceleration of Hydroxyl Radical Generation Peer-reviewed

    Midori Shirato, Hiroyo Ikai, Keisuke Nakamura, Eisei Hayashi, Taro Kanno, Keiichi Sasaki, Masahiro Kohno, Yoshimi Niwano

    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY　56　(1)　295-301　2012/01

    DOI： 10.1128/AAC.05158-11 　

    ISSN： 0066-4804

  81. Presence of hydrogen peroxide, a source of hydroxyl radicals, in acid electrolyzed water. International-journal Peer-reviewed

    Takayuki Mokudai, Keisuke Nakamura, Taro Kanno, Yoshimi Niwano

    PloS one　7　(9)　e46392　2012

    DOI： 10.1371/journal.pone.0046392 　

  82. Reevaluation of analytical methods for photogenerated singlet oxygen Peer-reviewed

    Keisuke Nakamura, Kirika Ishiyama, Hiroyo Ikai, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano, Masahiro Kohno

    JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION　49　(2)　87-95　2011/09

    DOI： 10.3164/jcbn.10-125 　

    ISSN： 0912-0009

    eISSN： 1880-5086

  83. Photolysis of Hydrogen Peroxide, an Effective Disinfection System via Hydroxyl Radical Formation Peer-reviewed

    Hiroyo Ikai, Keisuke Nakamura, Midori Shirato, Taro Kanno, Atsuo Iwasawa, Keiichi Sasaki, Yoshimi Niwano, Masahiro Kohno

    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY　54　(12)　5086-5091　2010/12

    DOI： 10.1128/AAC.00751-10 　

    ISSN： 0066-4804

  84. A novel analytical method to evaluate directly catalase activity of microorganisms and mammalian cells by ESR oximetry Peer-reviewed

    Keisuke Nakamura, Taro Kanno, Takayuki Mokudai, Atsuo Iwasawa, Yoshimi Niwano, Masahiro Kohno

    FREE RADICAL RESEARCH　44　(9)　1036-1043　2010/09

    DOI： 10.3109/10715762.2010.495750 　

    ISSN： 1071-5762

  85. Reevaluation of Quantitative ESR Spin Trapping Analysis of Hydroxyl Radical by Applying Sonolysis of Water as a Model System

    Keisuke Nakamura, Taro Kanno, Hiroyo Ikai, Emiko Sato, Takayuki Mokudai, Yoshimi Niwano, Toshihiko Ozawa, Masahiro Kohno

    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN　83　(9)　1037-1046　2010/09

    DOI： 10.1246/bcsj.20100078 　

    ISSN： 0009-2673

    eISSN： 1348-0634

  86. Zirconia as a dental implant abutment material: A systematic review Peer-reviewed

    Keisuke Nakamura, Taro Kanno, Percy Milleding, Ulf Örtengren

    International Journal of Prosthodontics　23　(4)　299-309　2010

    ISSN： 0893-2174

  87. Radical Sterilization Properties of Hydrogen Peroxide Activated by LED (386nm) Irradiation for Oral Bacterium : A Pilot Study

    KANNO Taro, NAKAMURA Keisuke, IKAI Hiroyo, MOKUDAI Takayuki, HAYASHI Eisei, KOHNO Masahiro

    Prosthodont Res. Pract.　7　(2)　138-140　2008/07/01

    Publisher: Japan Prosthodontic Society

    DOI： 10.2186/prp.7.138 　

    ISSN： 1347-7021

  88. What prosthodontic therapy should we select for periodontally compromised patients? Part 1: Review of the literature focusing on implant therapy for periodontally compromised patients Peer-reviewed

    Kanno T, Nakamura K, Hayashi E, Ikai H, Hirooka H, Kimura K

    Nihon Hotetsu Shika Gakkai zasshi　52　(2)　135-142　2008/04

    Publisher: Japan Prosthodontic Society

    DOI： 10.2186/jjps.52.135 　

    ISSN： 0389-5386

  89. What prosthodontic therapy should we select for periodontally compromised patients? Part 2: Review of the literature focusing on conventional prosthodontic therapy for periodontally compromised patients and clinical implication (RPD vs FPD vs implant) Peer-reviewed

    Kanno T, Nakamura K, Hayashi E, Ikai H, Hirooka H, Kimura K

    Nihon Hotetsu Shika Gakkai zasshi　52　(2)　143-149　2008/04

    Publisher: Japan Prosthodontic Society

    DOI： 10.2186/jjps.52.143 　

    ISSN： 0389-5386

  90. Galvanic corrosion of ferritic stainless steels used for dental magnetic attachments in contact with an iron-platinum magnet Peer-reviewed

    Keisuke Nakamura, Yukyo Takada, Masanobu Yoda, Kohei Kimura, Osamu Okuno

    Dental Materials Journal　27　(2)　203-210　2008

    DOI： 10.4012/dmj.27.203 　

    ISSN： 0287-4547

  91. Study on corrosion behavior of ferritic stainless steels used for dental magnetic attachement systems using an iron-platinum magnet. Peer-reviewed

    NAKAMURA Keisuke

    東北大学　2006

Show all ︎Show first 5

Misc. 92

  1. The efficacy of hydrogen peroxide photolysis on infected dentin

    Shirato M., Nakamura K., Lingström P., rtengren U.

    103rd General Session & Exhibition of the IADR in Barcelona, Spain　2025

  2. Encapsulated sugar - A novel method for sugar administration

    Lingström P., Alshabeeb A., Nakamura K., Lehrkinder A.

    CED/NOF-IADR Oral Health Research Congress in Geneva, Switzerland　2024

  3. Impact of hydrogel-encapsulated sugar on dental caries incidence in rats

    Nakamura K., Shirato M., Shunichi S., Lingström P.

    CED/NOF-IADR Oral Health Research Congress in Geneva, Switzerland　2024

  4. Reduction of bacterial loads in dental plaque by H2O2 photolysis

    Shirato M., Lehrkinder A., Nakamura K., Lingström P., Örtengren U.

    CED/NOF-IADR Oral Health Research Congress in Geneva, Switzerland　2024

  5. Residual stress on translucent zirconia subjected to sandblasting and firing

    Shishido S., Nakamura K., Svanborg P., Barkarmo S., rtengren U.

    CED/NOF-IADR Oral Health Research Congress in Geneva, Switzerland　2024

  6. 感染歯質に対する過酸化水素光分解殺菌技術の殺菌効果

    白土翠, 中村圭祐, 菅野太郎

    日本防菌防黴学会 第51回学術集会　2024

  7. 患者用アプリ「ペリミル」開発の背景とその臨床的意義

    菅野太郎, 石山希里香, 中村圭祐

    歯界展望　144　162-170　2024

  8. 新規歯周病治療器ブルーラジカル P-01：基礎研究および臨床試験データの解説

    中村圭祐, 石山希里香, 菅野太郎

    歯界展望　143　1173-1181　2024

  9. 新規歯周病治療器ブルーラジカル P-01：研究開発の学術的背景と研究結果の解説

    中村圭祐, 石山希里香, 菅野太郎

    歯界展望　143　967-974　2024

  10. ブルーラジカル P-01 & ペリミル

    菅野太郎, 石山希里香, 中村圭祐

    歯界展望　143　760-766　2024

  11. 1.6‐テラヘルツ（THｚ）レーザー照射が細菌に与える影響

    白土翠, 瀧田佑馬, 菅野太郎, 松浦寛, 庭野吉己, 南出泰亜, 中村圭祐

    日本防菌防黴学会 第50回学術集会　2023

  12. Comparison in education and management of the caries disease between two universities

    Åkerlind K., Tu L., Nakamura K., Hong G., Örtengren U.

    International Symposium for Interface Oral Health Science 2022　2022

  13. Treatment with hydrogen peroxide photolysis inhibits tooth demineralization caused by Streptococcus mutans biofilm

    Shirato M., Nakamura K., Tenkumo T., Niwano Y., Kanno T., Sasaki K., Lingström P., Örtengren U.

    International Symposium for Interface Oral Health Science 2022　2022

  14. The mechanical and microstructural properties of self-glazed zirconia

    Shishido S., Hua Y., Chhetri PK, Nakamura K., Svanborg P.

    International Symposium for Interface Oral Health Science 2022　2022

  15. Bacillus cereus芽胞に対する過酸化水素光分解殺菌法の殺芽胞効果

    宍戸駿一, 白土翠, 倉内美智子, 中村圭祐, 菅野太郎, 庭野吉己

    日本防菌防黴学会 第49回学術集会　2022

  16. 活性酸素発生を応用した口腔細菌制御技術の歯科応用 Invited

    中村圭祐

    本防菌防黴学会 第49回学術集会　2022

  17. ヒドロキシルラジカルを応用した殺菌技術の歯科臨床応用 Invited

    中村圭祐

    日本機能水学会 第20回学術大会　2022

  18. Dental application of ultraviolet-A induced photochemical and photobiological reactions Invited

    Nakamura K.

    2022 TU-HKU-FJMU International Symposium on Oral Health Sciences　2022

  19. ワイヤグリッド偏光子付き光ファイババンドルを用いた拡散反射計測による歯質判別

    岡谷泰佑, 横山雄基, 宍戸駿一, 中村圭祐, 金森義明

    第39回「センサ・マイクロマシンと応用システム」シンポジウム　2022

  20. カテキンの光酸化反応機構の解明および活性酸素を介した殺菌活性評価

    宍戸駿一, 宮野怜, 中島琢自, 松尾洋孝, 岩月正人, 中村圭祐, 菅野太郎, 江草宏, 庭野吉己, 庭野吉己

    日本ポリフェノール学会学術集会プログラム・講演抄録集　15th　2022

  21. Improvement of bond strength between resin cement and zirconia by surface treatment with low-temperature atmospheric pressure multi-gas plasma

    依田信裕, 松舘芳樹, 星野智大, 菅野武彦, 中村圭祐, 佐々木啓一, 阿部優凜, 末永祐磨, 沖野晃俊

    生体医歯工学共同研究拠点成果報告書　2021　2022

  22. Improvement of bond strength between resin cement and zirconia by surface treatment with low-temperature atmospheric pressure multi-gas plasma

    依田信裕, 松舘芳樹, 星野智大, 菅野武彦, 中村圭祐, 佐々木啓一, 阿部優凛, 末永祐磨, 沖野晃俊

    生体医歯工学共同研究拠点成果報告会　2021 (CD-ROM)　2022

  23. Improvement of bone health condition by oral administration of proanthocyanidin-rich grape seed extract in ovariectomized animals

    Nakamura K., Tenkumo T., Aobulikasimu A., Shirato M., Shishido S., Kanno T., Niwano Y., Sasaki K., Asou Y.

    XXX International Conference on Polyphenols (ICP2020 Turku)　2021

  24. A reaction mechanism of photo-oxidation process of catechin in relation to its bactericidal activity

    Shishido S., Miyano R., Nakashima T., Matsuo H., Iwatsuki M., Nakamura K., Kanno T., Egusa H., Niwano Y.

    XXX International Conference on Polyphenols (ICP2020 Turku)　2021

  25. 過酸化水素光分解殺菌法によるう蝕治療有効性評価のための多菌種バイオフィルムモデルの検討

    白土翠, 中村圭祐, 菅野太郎, 庭野吉己

    第48回日本防菌防黴学会年次大会　2021

  26. Applications of a novel antimicrobial chemotherapy utilizing HO generated by H2O2 photolysis to dental treatments Invited

    Nakamura K.

    2020 TU-HKU-FJMU International Symposium on Oral Health Sciences　2020

  27. Time-kill kinetic analysis of antimicrobial chemotherapy based on hydrogen peroxide photolysis

    Örtengren U., Shirato M., Lingström P., Nakamura K.

    The Annual Swedish Dental Congress of 2020　2020

  28. 医師主導治験におけるラジカル殺菌歯周病治療器の臨床効果の検証 無作為化比較試験 Peer-reviewed

    佐々木 啓一, 菅野 太郎, 中村 圭祐, 石山 希里香, 山田 康友, 白土 翠, 庭野 吉己, 萱場 千恵, 池田 浩治, 高木 愛理, 山口 拓洋

    日本歯周病学会会誌　61　(春季特別)　127-127　2019/05

    Publisher: (NPO)日本歯周病学会

    ISSN： 0385-0110

    eISSN： 1880-408X

  29. 歯周病原因菌によって汚染されたチタン表面の清浄化技術の開発：過酸化水素光分解で生成する水酸化ラジカルの応用

    白土翠, 中村圭祐, 天雲太一, 菅野太郎, 佐々木啓一, 庭野吉己

    第46回日本防菌防黴学会年次大会　2019

  30. 遺伝子導入剤を含有したナノハイドロキシアパタイトーコラーゲンscaffoldの開発

    天雲 太一, 中村 圭祐, 佐々木 啓一, 菅野 太郎

    日本歯周病学会会誌　60　(春季特別)　130-130　2018/05

    Publisher: (NPO)日本歯周病学会

    ISSN： 0385-0110

  31. Gene transfection in vivo with bone morphogenetic protein-2 encoding DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds

    Tenkumo T., Saenz JRV, Nakamura K., Shimizu Y., Sokolova V., Epple M., Kamano Y., Egusa H., Sugaya T., Sasaki K.

    International Symposium for Multimodal Research and Education in IOHS-Liaison 2018 in Sendai, Japan　2018

  32. Reconditioning of biofilm-contaminated titanium surface for osteoblast proliferation by hydroxyl radicals generated via H2O2 photolysis

    Nakamura K., Shirato M., Tenkumo T., Kanno T., Westerlund A., Örtengren U., Sasaki K., Niwano Y.

    International Symposium for Multimodal Research and Education in IOHS-Liaison 2018 in Sendai, Japan　2018

  33. プロタミンを付与したリン酸カルシウム系遺伝子導入剤の抗菌作用

    天雲太一, 中村圭祐, 白土翠, 菅野太郎, 佐々木啓一

    第45回日本防菌防黴学会年次大会　2018

  34. Streptococcus mutansバイオフィルムが引き起こす歯質脱灰に対する過酸化水素光分解殺菌技術の効果

    白土翠, 中村圭祐, 天雲太一, 菅野太郎, 庭野吉己

    第45回日本防菌防黴学会年次大会　2018

  35. カフェイン酸に対するUV-A照射で得られる殺菌作用と口腔粘膜刺激性の評価

    中村圭祐, 天雲太一, 目代貴之, 白土翠, 石山希里香, 菅野太郎, 佐々木啓一, 庭野吉己

    日本防菌防黴学会年次大会要旨集　45th　2018

  36. カテキンの光酸化を介した殺菌活性と光酸化機序の解明-その2-

    宍戸駿一, 宮野怜, 中島琢自, 松尾洋孝, 岩月正人, 中村圭祐, 菅野太郎, 江草宏, 庭野吉己

    日本防菌防黴学会年次大会要旨集　45th　2018

  37. 齲蝕原因菌（Streptococcus mutans）のバイオフィルムに対する長波長紫外線照射の殺菌作用

    中村圭祐, 白土翠, 菅野太郎, 庭野吉己

    日本防菌防黴学会 第44回年次大会　2017

  38. Comparison of antimicrobial activity of hydrogen peroxide photolysis against Streptococcus mutans biofilm with photodynamic therapy

    Shirato M., Nakamura K., Kanno T., Lingström P., Niwano Y., rtengren U.

    CED-IADR/NOF Oral Health Research Congress in Vienna, Austria　2017

  39. Anti-biofilm effect of hydroxyl radicals generated in UVA-irradiated caffeic acid

    Nakamura K., Shirato M., Kanno T., Lingström P., Örtengren U., Niwano Y.

    CED-IADR/NOF Oral Health Research Congress in Vienna, Austria　2017

  40. カテキンの光酸化を介した殺菌活性と光酸化機序の解明

    宍戸駿一, 宮野怜, 中島琢自, 中島琢自, 佐藤恵美子, 中村圭祐, 菅野太郎, 江草宏, 庭野吉己

    日本防菌防黴学会年次大会要旨集　44th　2017

  41. 過酸化水素光分解殺菌技術における光の波長と過酸化水素濃度の相乗作用

    倉内美智子, 唐木俊英, 中村圭祐, 菅野太郎, 勝田悠介, 猪飼紘代, 林栄成, 佐々木啓一, 庭野吉己

    日本防菌防黴学会年次大会要旨集　41st　2014

  42. 過酸化水素光分解殺菌技術を応用した新規歯周病治療器の安全性と有効性の検証

    猪飼紘代, 目代貴之, 中村圭祐, 菅野太郎, 庭野吉己

    日本防菌防黴学会年次大会要旨集　41st　2014

  43. 細菌、ウィルスに対するPDTの研究成果 歯垢染色剤に含まれる各種キサンテン系色素を光感受性物質とした一重項酸素依存性光線力学殺菌療法の比較 Peer-reviewed

    庭野 吉己, 石山 希里香, 中村 圭祐, 猪飼 紘代, 菅野 太郎, 佐々木 啓一

    日本レーザー医学会誌　34　(3)　326-326　2013/10

    Publisher: (NPO)日本レーザー医学会

    ISSN： 0288-6200

    eISSN： 1881-1639

  44. 過酸化水素の光分解および水の超音波分解を併用したヒドロキシルラジカル生成系の殺菌効果 Peer-reviewed

    生 宏, 中村 圭祐, 菅野 太郎, 佐々木 啓一, 庭野 吉己

    超音波医学　40　(4)　427-427　2013/07

    Publisher: (公社)日本超音波医学会

    ISSN： 1346-1176

    eISSN： 1881-9311

  45. 過酸化水素光分解殺菌技術を応用した新規歯周病治療器の開発 Peer-reviewed

    菅野 太郎, 中村 圭祐, 猪飼 紘代, 庭野 吉己, 佐々木 啓一

    日本歯周病学会会誌　55　(春季特別)　90-90　2013/04

    Publisher: (NPO)日本歯周病学会

    ISSN： 0385-0110

    eISSN： 1880-408X

  46. 電気分解で生成された酸性電解水中に存在する水酸化ラジカルは殺菌活性に寄与するか?

    目代貴之, 中村圭祐, 菅野太郎, 庭野吉己

    日本防菌防黴学会年次大会要旨集　40th　2013

  47. 過酸化水素光分解殺菌法に対する熱エネルギーの相乗的効果 Peer-reviewed

    白土 翠, 猪飼 紘代, 中村 圭祐, 林 栄成, 菅野 太郎, 佐々木 啓一, 庭野 吉己

    日本歯科医師会雑誌　65　(5)　615-615　2012/08

    Publisher: (公社)日本歯科医師会

    ISSN： 0047-1763

  48. プラーク染色剤を用いた新しい光線力学殺菌療法の基礎的検討 Peer-reviewed

    石山 希里香, 中村 圭祐, 猪飼 紘代, 菅野 太郎, 佐々木 啓一, 庭野 吉己

    日本歯科医師会雑誌　65　(5)　615-615　2012/08

    Publisher: (公社)日本歯科医師会

    ISSN： 0047-1763

  49. 過酸化水素光分解殺菌法に対する微生物の抵抗性とカタラーゼ活性の関連性

    中村 圭祐, 菅野 太郎, 目代 貴之, 林 栄成, 猪飼 紘代, 佐々木 啓一, 庭野 吉己

    日本歯科医師会雑誌　65　(5)　615-615　2012/08

    Publisher: (公社)日本歯科医師会

    ISSN： 0047-1763

  50. 過酸化水素光分解を応用したラジカル殺菌技術のin vivo殺菌活性および安全性評価

    庭野吉己, 林栄成, 山田康友, 目代貴之, 中村圭祐, 菅野太郎, 佐々木啓一

    日本防菌防黴学会年次大会要旨集　39th　2012

  51. 過酸化水素光分解を利用した新規歯科治療器のラット口腔粘膜および創傷皮膚への局所応用における急性の毒性学的評価

    山田康友, 目代貴之, 中村圭祐, 林栄成, 菅野太郎, 佐々木啓一, 庭野吉己

    日本酸化ストレス学会学術集会プログラム・抄録集　65th　2012

  52. 過酸化水素光分解を応用した新しい殺菌システムの黄色ブドウ球菌に対するin vitroおよびin vivo抗菌活性

    林栄成, 目代貴之, 山田康友, 中村圭祐, 菅野太郎, 佐々木啓一, 庭野吉己

    日本酸化ストレス学会学術集会プログラム・抄録集　65th　2012

  53. Comparison of different analytical methods for determining singlet oxygen photogenerated from rose bengal

    Kirika Ishiyama, Keisuke Nakamura, Hiroyo Ikai, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano, Masahiro Kohno

    Interface Oral Health Science 2011　89-91　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_15 　

  54. Corrosion resistance of dental metals to hydroxyl radical generated by photolysis of H2O2

    Yasutomo Yamada, Keisuke Nakamura, Yukyo Takada, Takayuki Mokudai, Hiroyo Ikai, Ryoichi Inagaki, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano, Masahiro Kohno

    Interface Oral Health Science 2011　240-242　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_68 　

  55. Bactericidal effect of hydroxyl radical generated by photolysis of hydrogen peroxide

    Hiroyo Ikai, Keisuke Nakamura, Midori Shirato, Taro Kanno, Atsuo Iwasawa, Yoshimi Niwano, Keiichi Sasaki, Masahiro Kohno

    Interface Oral Health Science 2011　86-88　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_14 　

  56. Booster effect of thermal energy on bactericidal action of hydroxyl radical generated by photolysis of H2O2

    Midori Shirato, Hiroyo Ikai, Keisuke Nakamura, Eisei Hayashi, Taro Kanno, Keiichi Sasaki, Masahiro Kohno, Yoshimi Niwano

    Interface Oral Health Science 2011　150-152　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_38 　

  57. ヒドロキシルラジカルが歯科用金属の耐食性に及ぼす影響

    山田康友, 中村圭祐, 高田雄京, 目代貴之, 猪飼紘代, 稲垣亮一, 菅野太郎, 佐々木啓一, 庭野吉己, 河野雅弘

    日本酸化ストレス学会学術集会プログラム・抄録集　64th　2011

  58. 過酸化水素光分解殺菌法における微生物の反応動態解析

    林栄成, 中村圭祐, 菅野太郎, 目代貴之, 岩沢篤朗, 河野雅弘, 庭野吉己

    日本酸化ストレス学会学術集会プログラム・抄録集　64th　2011

  59. Denture Plaque Removal Efficacy of Denture Cleansing System Utilizing Radical Disinfection Ability of Low Concentration H_2O_2 Activated by Visible Radiation

    KANNO T, MOKUDAI T, HAYASHI E, IKAI H, NAKAMURA K, KUDOU A, KOHNO M, SASAKI K

    日本補綴歯科学会誌. 特別号, 社団法人日本補綴歯科学会学術大会プログラム・抄録集 = Program and abstracts, the ... scientific meeting of Japan Prosthodontic Society　1　(118)　2010/08/16

    ISSN： 1883-4426

  60. ESRスピントラップ法を用いたラジカル定量の潜在的問題

    佐藤恵美子, 中村圭祐, 菅野太郎, 猪飼紘代, 目代貴之, 庭野吉己, 小澤俊彦, 河野雅弘

    日本酸化ストレス学会学術集会プログラム・抄録集　63rd　128　2010/06/24

  61. ESRオキシメトリー法を応用した微生物および動物細胞の新しいカタラーゼ活性測定法

    中村圭祐, 中村圭祐, 菅野太郎, 目代貴之, 岩沢篤郎, 岩沢篤郎, 庭野吉己, 河野雅弘

    日本酸化ストレス学会学術集会プログラム・抄録集　63rd　2010

  62. Transmitted laser beam power of the resin washed by experimental washing machine for dentures Peer-reviewed

    Eisei Hayashi, Mika Tada, Taro Kanno, Hiroyo Ikai, Keisuke Nakamura, Masahiro Kohno

    INTERFACE ORAL HEALTH SCIENCE 2009　257-+　2010

    DOI： 10.1007/978-4-431-99644-6_69 　

  63. The evaluation of the dental disinfection device with low concentration of H2O2 and laser diode Peer-reviewed

    Hiroyo Ikai, Taro Kanno, Keisuke Nakamura, Eisei Hayashi, Akihito Kudo, Masahiro Kohno

    INTERFACE ORAL HEALTH SCIENCE 2009　259-+　2010

    DOI： 10.1007/978-4-431-99644-6_70 　

  64. Bactericidal effect of photodynamic therapy Peer-reviewed

    Keisuke Nakamura, Mika Tada, Taro Kanno, Hiroyo Ikai, Eisei Hayashi, Takayuki Mokudai, Masahiro Kohno

    INTERFACE ORAL HEALTH SCIENCE 2009　232-+　2010

    DOI： 10.1007/978-4-431-99644-6_59 　

  65. Denture plaque removal efficacy of denture cleansing device utilizing radical disinfection ability of activated low concentration H2O2 Peer-reviewed

    Taro Kanno, Eisei Hayashi, Hiroyo Ikai, Keisuke Nakamura, Takayuki Mokudai, Masahiro Kohno, Keiichi Sasaki

    INTERFACE ORAL HEALTH SCIENCE 2009　252-+　2010

    DOI： 10.1007/978-4-431-99644-6_67 　

  66. 化学発光における末梢血好中球と口腔内好中球の細胞応答の違い

    八重柏典子, 佐藤恵美子, 中村圭祐, 猪飼紘代, 林栄成, 菅野太郎, 河野雅弘

    日本酸化ストレス学会学術集会プログラム・抄録集　62nd　117　2009/06/11

  67. 光励起された電解水および過酸化水素の殺菌効果

    菅野 太郎, 古田 美香, 目代 貴之, 中村 圭祐, 岩沢 篤朗, 河野 雅弘

    日本口腔機能水学会誌　10　(1)　45-46　2009/03

    Publisher: 日本口腔機能水学会

  68. 塩素・酸素複合体に関与する活性種の研究

    目代貴之, 河野雅弘, 中村圭祐, 岩沢篤郎

    日本防菌防黴学会年次大会要旨集　36th　2009

  69. 低濃度過酸化水素に405nm可視光を照射することにより生成したヒドロキシルラジカルの殺菌効果

    中村圭祐, 目代貴之, 菅野太郎, 林栄成, 猪飼紘代, 河野雅弘, 岩沢篤郎

    日本防菌防黴学会年次大会要旨集　36th　2009

  70. Denture Plaque Removal Efficacy of Denture Cleansing Device Utilizing Radical Ssterilization Ability of Activated Low Hypodense Hydrogen Peroxide

    KANNO T, HAYASHI E, IKAI H, NAKAMURA K, MOKUDAI T, KUDOU A, KOHNO M, SASAKI K

    日本補綴歯科学会雑誌. 特別号, 日本補綴歯科学会学術大会抄録集 = Proceedings of the ... conference, the Japan Prosthodontic Society　52　(117)　2008/06/06

    ISSN： 1883-4426

  71. Radical Sterilization Properties of Hydrogen Peroxide Activated by LED (386nm) Irradiation for Candida Albicans

    IKAI H, KANNO T, NAKAMURA K, HAYASHI E, MOKUDAI T, IWASAWA A, KUDOU A, KOHNO M

    日本補綴歯科学会雑誌. 特別号, 日本補綴歯科学会学術大会抄録集 = Proceedings of the ... conference, the Japan Prosthodontic Society　52　(117)　2008/06/06

    ISSN： 1883-4426

  72. New Irradiation System for Clinical Dental Treatment Advanced Using Low Density Hydrogen Peroxide and Laser Diode

    IKAI H, KANNO T, HAYASHI E, NAKAMURA K, MOKUDAI T, KUDOU A, IWASAWA A, KOHNO M

    日本補綴歯科学会雑誌. 特別号, 日本補綴歯科学会学術大会抄録集 = Proceedings of the ... conference, the Japan Prosthodontic Society　52　(117)　2008/06/06

    ISSN： 1883-4426

  73. 活性酸素・フリーラジカルを応用した化学的なプラーク除去(第3報)

    林 栄成, 菅野 太郎, 目代 貴之, 猪飼 紘代, 中村 圭祐, 木村 幸平, 河野 雅弘

    日本補綴歯科学会雑誌　52　(2)　274-274　2008/04

    Publisher: (公社)日本補綴歯科学会

    ISSN： 0389-5386

    eISSN： 1883-177X

  74. 電解機能水と特定周波数の超音波を併用したプラーク除去法

    林 栄成, 菅野 太郎, 猪飼 紘代, 目代 貴之, 中村 圭祐, 河野 雅弘

    日本口腔機能水学会誌　9　(1)　36-36　2008/03

    Publisher: 日本口腔機能水学会

  75. 活性酸素・フリーラジカルを応用した化学的なプラーク除去(第1報)

    猪飼 紘代, 菅野 太郎, 目代 貴之, 林 栄成, 中村 圭祐, 河野 雅弘, 木村 幸平

    日本補綴歯科学会雑誌　51　(116回特別)　155-155　2007/05

    Publisher: (公社)日本補綴歯科学会

    ISSN： 0389-5386

    eISSN： 1883-177X

  76. 活性酸素・フリーラジカルを応用した化学的なプラーク除去(第2報)

    林 栄成, 菅野 太郎, 目代 貴之, 猪飼 紘代, 中村 圭祐, 河野 雅弘, 木村 幸平

    日本補綴歯科学会雑誌　51　(116回特別)　156-156　2007/05

    Publisher: (公社)日本補綴歯科学会

    ISSN： 0389-5386

    eISSN： 1883-177X

  77. 活性酸素・フリーラジカルを応用した化学的なプラーク除去-第1報-

    猪飼紘代, 菅野太郎, 目代貴之, 林栄成, 中村圭祐, 河野雅弘, 木村幸平

    日本補綴歯科学会学術大会プログラム・抄録集　116th　2007

    ISSN： 1883-4426

  78. 活性酸素・フリーラジカルを応用した化学的なプラーク除去-第2報-

    林栄成, 菅野太郎, 目代貴之, 猪飼紘代, 中村圭祐, 河野雅弘, 木村幸平

    日本補綴歯科学会学術大会プログラム・抄録集　116th　2007

    ISSN： 1883-4426

  79. はたして過剰な咬合力は歯科用インプラントの失敗を招くのか？ -Scandinavian Consensus- (日本実験力学会誌)

    菅野太郎, 中村圭祐, 猪飼紘代, 林栄成

    日本実験力学会誌　6　(2)　82-87　2006

  80. Does the excessive occlusal load cause the failure of the dental implant? -Scandinavian Consensus- (The Japanese Society for Experimental Mechanics)

    Kanno Taro, Nakamura Keisuke, Ikai Hiroyo, Hayashi Eisei

    The Japanese Society for Experimental Mechanics　6　(2)　82-87　2006

  81. 磁性アタッチメント用ステンレス鋼の腐食溶出挙動

    高田雄京, 中村圭祐, 木村幸平, 奥野攻

    日本磁気歯科学会雑誌　14　(1)　94-95　2005/10/01

    Publisher: 日本磁気歯科学会

    ISSN： 0918-9629

  82. Corrosion behavior of the stainless steel composing dental magnetic attachments

    Yukyo Takada, Keisuke Nakamura, Kohei Kimura, Osamu Okuno

    International Congress Series　1284　314-315　2005/09

    DOI： 10.1016/j.ics.2005.06.010 　

    ISSN： 0531-5131

  83. Effects of heat treatment on corrosion behavior of SUS447J1 castings

    NAKAMURA K., TAKADA Y., KIMURA K., OKUNO O.

    Journal of the Japanese Society for Dental Materials and Devices　24　(2)　187-187　2005/04/05

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  84. Effects of heat treatment on anodic behavior of magnetic stainless steel castings in electrochemical evaluations

    TAKADA Y., NAKAMURA K., KIMURA K., OKUNO O.

    Journal of the Japanese Society for Dental Materials and Devices　23　(5)　350-350　2004/09/10

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  85. 6. アルジネート印象の採得方法と保管方法の相違による咬合再現性の臨床的評価(第44回東北大学歯学会講演抄録)

    片岡 展夫, 菅野 太郎, 猪飼 紘代, 中村 圭祐, 三宅 理史, 木村 幸平, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野

    東北大学歯学雑誌 = Tohoku University dental journal　23　(1)　37-37　2004/06/30

    ISSN： 0287-3915

  86. Effects of Cr contents on galvanic corrosion of magnetic stainless steels in contact with the platinum-iron magnetic alloy

    Nakamura Keisuke, Haoka Katsunori, Takada Yukyo, Kimura Kohei, Okuno Osamu

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2004　(0)　115-115　2004/04

    Publisher: 日本歯科理工学会

    DOI： 10.14832/gsjsdmd.2004s.0.115.0 　

    ISSN： 0286-5858

    More details Close

    Cr含有量の異なる3種の磁性ステンレス鋼のSUS444，SUSXM27，SUS447J1と白金鉄磁石合金（Fe-39.5at%Pt-0.75at%Nb）を1:1および1:10の表面積比で接触し、溶存酸素を飽和にした37℃の0.9％NaCl水溶液における溶出イオンを定量分析した。ステンレス鋼のCr含有量と溶出イオン量の関係から、耐食性の面で白金鉄磁石合金と組み合わせ可能な磁性ステンレス鋼を検討した。

  87. Effects of Cr contents on galvanic corrosion of magnetic stainless steels in contact with the platinum-iron magnetic alloy

    NAKAMURA K., HAOKA K., TAKADA Y., KIMURA K., OKUNO O.

    Journal of the Japanese Society for Dental Materials and Devices　23　(2)　171-171　2004/03/20

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  88. 科学的根拠に基づくこれからの歯周病治療の潮流 -神奈川歯科大学同窓会学術講演会より- (歯界展望)

    菅野太郎, 中村圭祐

    歯界展望　104　(6)　1295-1299　2004

  89. The modality of the periodontal therapy based on the evidence. - from the lecture of the alumni association of Kanagawa Dental Univ.- (Dental Outlook)

    Kanno Taro, Nakamura Keisuke

    Dental Outlook　104　(6)　1295-1299　2004

  90. 4. 咬合採得における再現性について : 採得方法のトレーニング前後および採得材料間の相違(第43回東北大学歯学会講演抄録)

    片岡 展夫, 菅野 太郎, 猪飼 紘代, 中村 圭祐, 三宅 埋史, 木村 幸平, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野, 東北大学大学院歯学研究科口腔修復学講座咬合機能再建学分野

    東北大学歯学雑誌 = Tohoku University dental journal　22　(2)　127-127　2003/12/30

    ISSN： 0287-3915

  91. インプラント治療における生物学 -Lindhe教授講演会より- (歯界展望)

    菅野太郎, 中村圭祐

    歯界展望　101　(5)　1141-1145　2003

  92. The biology of implant therapy - from the lecture of Prof. Lindhe.- (Dental Outlook)

    Kanno Taro, Nakamura Keisuke

    Dental Outlook　101　(5)　1141-1145　2003

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Books and Other Publications 3

  1. オクルージョン＆クリニカルプラクティス ―エビデンスに基づいたアプローチ―

    中村 圭祐

    医歯薬出版株式会社　2007

  2. コレクテッドエビデンス〈vol.2〉(Postgraduate program in clinical periodontology)

    中村 圭祐

    デンタルダイヤモンド社　2007

  3. コレクテッドエビデンス〈vol.1〉 (Postgraduate program in clinical periodontology)

    中村 圭祐

    デンタルダイヤモンド社　2007

Research Projects 23

  1. ウルトラファインバブルの医用応用に資する生物学的効果とトリガー援用増強効果の解明

    佐々木 啓一, 中村 圭祐, 松浦 寛, 伊賀 由佳, 久慈 千栄子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2025/04 - 2028/03

  2. 超高齢社会におけるプラーク制御法の確立－活性酸素を応用したバイオフィルム不活化－

    白土 翠, 宍戸 駿一, 中村 圭祐

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  3. Reconditioning of implant surface contaminated by microorganisms using advanced oxidation process

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2022/04/01 - 2025/03/31

  4. Tertiary dentin formation via hormesis effect induced by black-light irradiation

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2022/04/01 - 2025/03/31

  5. レーザ誘起微細周期構造の構造制御ダイナミクスと構造／生体組織間相互作用の解明

    水谷 正義, 中村 圭祐, 金高 弘恭

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  6. Facilitation of wound healing by proanthocyanidin: Application in gerodontology

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2021/04/01 - 2024/03/31

  7. Mechanistic analysis of bone formation via photobiologic reaction triggered by UVA irradiation

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2021/04/01 - 2024/03/31

  8. Improvement of success rate of dental implant in menopausal women: Development of novel technology for enhancing bone-implant osseointegration

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Shumei University

    2020/04/01 - 2023/03/31

  9. リン酸カルシウム遺伝子導入剤による機能性抗菌コーティングのインプラント周囲炎応用

    天雲 太一, 石山 希里香, 中村 圭祐, 白土 翠

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04/01 - 2023/03/31

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    インプラント治療は表面性状の発展に伴い治療の成功率が飛躍的に向上することで、世界的に普及する治療方法となった半面、インプラント周囲炎の報告も増加している。しかし、インプラント周囲炎に対する有効な治療方法はまだないことが歯科治療において大きな問題となっている。インプラント治療では、細菌などで汚染した表面の殺菌療法だけでは不十分で、その後に骨の再付着を誘導するようなシステムの確立が重要となる。そこで、本研究ではラジカル殺菌処置後に抗菌性リン酸カルシウム遺伝子導入ナノ粒子によるコーティング層を構築することで、再感染予防と再オステオインテグレーションの獲得をする新しいインプラント周囲組織再生療法を確立することを目的としている。まず、「リン酸カルシウムコーティング層の作製条件」を検証したところ、照射強度や照射時間は一定量を超えると、リン酸カルシウムの粒子の付着量に変化がなくなり、上限があることが明らかにしてきた。また、殺菌効果については、同じ照射強度、照射時間でも対象となる細菌の濃度には適正濃度があること、その適正濃度は細菌の種類によって異なることが明らかとなった。一方、「CaP コーティング層の表面性状、生体親和性」について解析したところ、菌種によってはリン酸カルシウム自体が細菌の増殖を助長していることが確認された。そこで、新たにこれまでと異なる抗菌ペプチドを付与したリン酸カルシウムナノ粒子を製作し、同細菌に対して殺菌試験を行ったところ、リン酸カルシウム自体による細菌増殖効果が抑制されることが明らかとなった。現在は同リン酸カルシウムナノ粒子の遺伝子導入効率や生体親和性をインプラント周囲に存在する歯周組織関連細胞を用いて検証している。

  10. Comprehensive analysis of high-translucent dental zirconia ceramics

    Inagaki Ryoichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    The present study aimed to investigate mechanical and microstructural properties of high translucent dental zirconia. The translucency of zirconia largely depends on the concentration of yttria, and as such, high translucent zirconia contained 4-6 mol% yttria. We demonstrated that the higher the yttria concentration, the larger amount of pseudo-cubic phase was generated in the zirconia. In addition, as the pseudo-cubic phase was more stable than tetragonal phase, the material with an increased concentration of yttria had higher resistance to hydro-thermal degradation. On the other hand, the increased yttria concentration decreased mechanical strength of the material. Overall, the high translucent zirconia possessed sufficient mechanical strength, even though it showed lower strength than conventional 3 mol% yttria-stabilized zirconia.

  11. Development of a novel dental caries therapy based on hydrogen peroxide photolysis

    Nakamura Keisuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    The aim of the present study was to evaluate 1) the antimicrobial activity of hydrogen photolysis treatment against multi-species biofilm containing cariogenic bacteria, and 2) reactions of the dental pulp to the treatment. The hydrogen photolysis treatment is a novel antimicrobial technique in which oxidative power of hydroxyl radicals generated by photo-irradiation of hydrogen peroxide is used. The treatment could effectively reduce viable bacteria in the multi-species biofilm as in the case of single-species biofilm. The reactions of the dental pulp were evaluated in an animal study using rats. The treatment generated localized degenerative tissue at pulp horn region, whereas infiltration of inflammatory cells was not observed. The degenerative tissue was repaired within 5 days, and tertiary dentin formation was observed 21 days after treatment.

  12. Improvement of implant osseointegration in osteoporosis patients via modification of intestinal microbiota

    Kanno Taro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    The present study aimed to evaluate the potential effects of proanthocyanidin-rich grape seed extract (GSE) on the healing of bone defects as well as implant osseointegration in ovariectomized (OVX) rats, a model of osteoporosis in postmenopausal women. Female Wistar rats were used. The animals underwent OVX operation. One week after OVX surgery, the second surgery was performed to create calvarial bone defects. In addition, one week after the second surgery, implant surgery was performed to install titanium mini-screws in tibiae. During the healing period, GSE or vehicle (water) was daily administered by oral gavage. OVX tended to impair healing of the defects. However, GSE counteracted the OVX-induced impairment in bone healing. Similarly, GSE increased the implant removal torque and bone-to-implant contact compared to those of the water-administered animals. These findings suggest that oral administration of GSE may be beneficial in dental implant therapy in osteoporosis patients.

  13. Development of surface modification process enabling adhesion with soft tissue for suppressing peri-implantitis

    Mizutani Masayoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

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    Dental health is closely related to quality of life (QOL) improvement, and implant therapy is effective in prosthesis for missing teeth and recovery of QOL. Currently, however, inflammation caused by bacterial infection is a problem in implant therapy. The purpose of this study is to create ideal functional bio-interfaces that have both biocompatibility and anti-bacterial adhesion properties, e.g. hydrophobicity and photocatalytic activity by the simultaneous process of microfabrication and chemical property changes on the material surface with laser processing. In conclusion, functional bio-interfaces for dental implants may be realized by forming grooves suppressing bacterial adhesion and suitable micro-irregularities promoting cell growth.

  14. research of prevention of low-temperature degradation of ful-countour zirconia prostheses

    Harada Akio, Egusa Hiroshi, Nakamura Keisuke, Nakazawa Kosuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    The purpose of this study was to evaluate the influence of ultrasonic scaling and low-temperature degradation (LTD) on surface properties of zirconia. Yttria-stabilized tetragonal zirconia polycrystals (3Y-TZP) were aged by autoclaving at 135℃ and 0.2 MPa for 100 hours comparable to 5 years intraoral aging, subsequently treated by ultrasonic scaler tips made of PEEK or stainless steel. X-ray diffraction analysis showed that LTD induced phase transformation whereas ultrasonic scaling did not generate monoclinic phase. Ultrasonic scaling significantly increased Sa (P <0.0001), especially when LTD-induced 3Y-TZP was subjected to ultrasonic scaling using stainless steel tip.

  15. Fatigue analysis of resin-based composite molar crowns fabricated via CAD/CAM technique

    Ryoichi Inagaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    In the present research project, strength, fatigue resistance, and crown retentive strenght of CAD/CAM-generated resin-based composite crowns were evaluated. Fatigue treatments, such as thermal cycling and mechanical cycling designed in relation to the clinical service of crowns in the oral cavity, were performed. The fatigue did not significantly affect the strength of the crown material (resin-based composite) and the crown fracture resistance. However, the fatigue decreased the crown retentive strength, increasing the risk of loss of retention. Thus, it is suggeted that future research and development should focus on prevention of crown loosening of CAD/CAM-generated resin-based composite crowns to achieve better clinical results.

  16. Basic study of peri-implantitis treatment aimed at reosseointegration using hydroxyl radical

    Shirato Midori, NIWANO yoshimi, NAKAMURA keisuke, TENKUMO taichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    This study aimed to evaluate the capability of treatment based on hydroxyl radical for peri-implantitis. In the study, sandblasted titanium discs were used as specimens. Firstly, treated specimens were observed to investigate the effect of this treatment on a titanium surface. There were not significant differences between treated discs and untreated discs. Then I analysed proliferation of mouse osteoblastic cells (MC3T3-E1) cultured on the treated discs. Compared with intact discs, biofilm (using Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum and Streptococcus mitis) contamination lowered cell proliferation on the specimen surface,whereas this treatment recovered cell proliferation. These findings suggest that treatment based on hydroxyl radical can recover the degraded biocompatibility of biofilm-contaminated titanium surfaces without damage and can potentially be utilised for peri-implantitis treatment.

  17. Devalopment of antimicrobial technique utilizing photo-irradiated polyphenol for oral care

    Keisuke Nakamura

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    In this project, in vitro and in vivo tests were performed to evaluate the antimicrobial chemotherapy utilizing photo-irradiated polyphenols. Streptococcus mutans biofilm was treated with the technique using caffeic acid, chlorogenic acid and proanthocyanidin as a representative polyphenol. The results showed that the technique using caffeic acid exerted the highest bactericidal activity. In vivo study where oral mucosal irritation potential of the antimicrobial technique was evaluated demonstrated that it exerted slight irritation potential. Therefore, in order to apply this technique to clinical dentistry, it is suggested that future studies should evaluate the bactericidal effect (benefit) and the oral mucosal irritation potential (risk) more in detail.

  18. Establishment of peri-implantitis treatment based on radical disinfection technique

    Sasaki Keiichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    In this project, in vitro and in vivo tests were performed with an aim of application of radical disinfection technique to treatment for peri-implantitis. In vivo test, where peri-implantitis caused around implants installed in dogs were treated with radical disinfection technique. However, there was no significant difference between the test and control groups. Thus, to find more suitable treatment conditions, in vitro test was additionally performed. The results demonstrated that the radical disinfection technique could recondition the titanium surface that had been previously contaminated by bacteria for subsequent osteoblastic cells. Therefore, future study should focus more on the establishment of treatment condition that enables to acquire re-osseointegration around the implants suffering from peri-implantitis.

  19. Mechanical properties of monolithic zirconia crowns

    Ryoichi Inagaki, NAKAMURA Keisuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    The present study aimed to evaluate the influence of crown thickness on the fracture resistance of monolithic zirconia crowns. In addition, the effects of aging (spontaneous crystalline phase transformation), cyclic loading and addition of coloring agents were investigated. The results demonstrated that monolithic zirconia crowns with a minimal thickness of 0.5 mm could work as molar crowns. Even though monolithic zirconia crowns were affected by aging and cyclic loading, the fracture load of the crown was sufficiently higher than maximum bite force (about 1000 N). Furthermore, it was shown that the coloring procedure would not affect the strength of zirconia. The results obtained in the present study should be verified in clinical studies in the future.

  20. A novel preventive technique of dental diseases based on photo-dynamic anti-microbial therapy utilizing plaque discolinsg liquid

    NAKAMURA Keisuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    Dyes contained in plaque disclosing liquid can be activated by photo-irradiation with a specific wavelength. The activated dyes generate singlet oxygen, a type of reactive oxygen species, when they return to the ground state. Since singlet oxygen has strong oxidative effect, it exerts bactericidal activity when reacts with bacteria. In the present study, it was demonstrated that the photodynamic antimicrobial therapy exerted strong bactericidal action on cariogenic bacteria (Streptococcus mutans). In addition, it was found that photo-irradiation of polyphenol aqueous solution also exerted bactericidal effect. Thus, it is suggested that this technique can be used a novel photodynamic antimicrobial therapy.

  21. Clinical application of a novel disinfection system based on local administration of low concentration hydroxyl radicals

    SASAKI Keiichi, KANNO Taro, HAYASHI Eisei, IKAI Hiroyo, NAKAMURA Keisuke, NIWANO Yoshimi, KOHNO Masahiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    Bactericidal effect and biological safety of a novel therapeutic device utilizing a disinfection technique based on photolysis of hydrogen peroxide for the treatment of periodontal disease were evaluated in the present study. In addition, clinical study was conducted to compare the efficacy of the device in the treatment of periodontitis with that of conventional root debridement. In vitro bactericidal tests demonstrated that device exerted high bactericidal effect, whereas in vivo test showed that it did not cause adverse effect on the oral mucosa of rats. In the clinical study, it was demonstrated that the treatment using the device significantly reduced the probing pocket depth 3 months after the treatment than conventional root debridement. Thus, it is suggested that the periodontal treatment using the device may improve the effect of non-surgical periodontal therapy.

  22. ヒドロキシルラジカルを人工的に生成する新しいラジカル殺菌技術の口腔内領域への応用

    佐々木 啓一, 菅野 太郎, 林 栄成, 猪飼 紘代, 中村 圭祐, 河野 雅弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2010 - 2011

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    〔目的〕 平成23年度は、本ラジカル殺菌技術のin vivoでの抗菌活性及び生体安全性を評価することを目的とした。 〔方法〕 殺菌活性評価:プレドニゾロン投与により免疫抑制状態にしたラット背部に直径1cmの全層皮膚欠損創を作製し、黄色ブドウ球菌を接種した。創作製の翌日(1日後)および2日後に1 M H_2O_2と青色LED光を2分間創傷部全体に処理した。その2日後に創傷部皮膚を摘出し、培養試験に供した。 安全性評価:ラットの口腔粘膜に1 M H_2O_2と青色レーザー光の2分間処理を3日間行い、粘膜組織に対する影響を調べた。加えて、ラット背部に作製した直径1cmの全層皮膚欠損創辺縁部に同様の処理を行い、その後の治癒過程の対する影響を調べた。 〔結果〕 殺菌活性評価:水だけを処理した対照群に対して1 M H_2O_2+青色LED光処理群では、回収された生菌数は約1/100(2logオーダー)と有意に減少した。水+青色LED光処理群、1 M H_2O_2単独処理群では殺菌効果は認められなかった。 安全性評価:1 M H_2O_2+青色レーザー光処理群の口腔粘膜組織像は、水だけを処理した対照群と同様であり、異常所見は認められなかった。ラット皮膚創傷モデルにおいても1 M H_2O_2+青色レーザー光処理群で治癒の遅延はなく、組織学的にも異常所見は認められなかった。 以上、in vitroで抗菌活性を発揮する条件で、本殺菌技術のin vivo表在性感染モデルでの抗菌活性を確認するとともに、局所粘膜および損傷皮膚への急性の影響を検討し、安全性に問題がないことを確認した。

  23. Development of a novel disinfection technique based on photodynamic therapy

    NAKAMURA Keisuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2009 - 2010

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    In the present study, it was demonstrated that singlet oxygen generated in photodynamic therapy could effectively kill Streptococcus aureus. In addition, a quantitative analytical method for singlet oxygen generated in photodynamic therapy has been established. It was demonstrated that electron spin resonance technique using sterically hindered amine was suitable for the measurement. The analysis showed that the yield of singlet oxygen increased with the irradiation time and the concentration of photosensitizer.

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