TOHOKU UNIVERSITY Researchers - Seiichi Nishizawa

Details of the Researcher

Home

日本語 English

Seiichi Nishizawa

Section

Graduate School of Science

Job title

Professor

Degree

博士（理学）（東京大学）
修士（理学）（北海道大学）

researchmap

https://researchmap.jp/6972

J-GLOBAL ID

200901052320015152

Research History 5

2013/04 - Present

東北大学大学院理学研究科化学専攻教授　教授
2007/04 - 2013/03

東北大学大学院理学研究科化学専攻准教授　准教授
2006/04 - 2007/03

東北大学大学院理学研究科化学専攻助教授　助教授
2004/04 - 2006/03

Lecturer, Department of Chemistry, Graduate School of Science, Tohoku University
1996/04 - 2004/03

Assistant Professor, Department of Chemistry, Graduate School of Science, Tohoku University　助手

Committee Memberships 30

日本化学会東北支部幹事会（平成29年度）　幹事

2017/02 - Present
日本化学会東北支部幹事会（平成29年度）　幹事

2017/02 - Present
日本化学会化学グランプリ・オリンピック委員会　幹事

2016/01 - Present
日本化学会化学グランプリ・オリンピック委員会　幹事

2016/01 - Present
日本化学会分析化学ディビジョン　幹事

2015/04 - Present
日本化学会分析化学ディビジョン　幹事

2015/04 - Present
日本分析化学会　東北支部常任幹事

2001/12 - Present
日本分析化学会　東北支部常任幹事

2001/12 - Present
Analytical Sciences 編集委員会　Associate Editor

2013/03 - 2015/02
Analytical Sciences 編集委員会　編集委員

2013/03 - 2015/02
日本化学会平成26年度化学グランプリ二次選考運営委員会　委員

2014/01 - 2014/10
日本化学会平成26年度化学グランプリ二次選考運営委員会　委員

2014/01 - 2014/10
日本化学会主催・平成25年度化学グランプリ・オリンピック委員会グランプリ小委員会　委員

2013/01 - 2013/10
日本化学会主催・平成25年度化学グランプリ・オリンピック委員会グランプリ小委員会　委員

2013/01 - 2013/10
日本分析化学会学会活性化戦略委員会　委員

2012/02 - 2013/02
日本分析化学会学会活性化戦略委員会　委員

2012/02 - 2013/02
日本学術振興会特別研究員等審査会　専門委員

2010/08 - 2012/07
日本学術振興会特別研究員等審査会　専門委員

2010/08 - 2012/07
第38回国際核酸化学シンポジウム組織委員会　委員

2011/01 - 2011/11
第38回国際核酸化学シンポジウム組織委員会　委員

2011/01 - 2011/11
平成23年度化学系学協会東北大会実行委員会　委員

2011/06 - 2011/09
平成23年度化学系学協会東北大会実行委員会　委員

2011/06 - 2011/09
日本分析化学会第59年会実行委員会　委員

2009/08 - 2010/12
日本分析化学会第59年会実行委員会　委員

2009/08 - 2010/12
日本化学会東北支部　幹事（会計）

2009/02 - 2010/02
日本化学会東北支部　幹事（会計）

2009/02 - 2010/02
第22回生体機能関連化学シンポジウム実行委員会　委員

2007/01 - 2007/09
第22回生体機能関連化学シンポジウム実行委員会　委員

2007/01 - 2007/09
IACIS2012（International Association of Colloid and Interface Scientists)、現地実行委員会　委員

2012/05 -
IACIS2012（International Association of Colloid and Interface Scientists)、現地実行委員会　委員

2012/05 -

Show all ︎Show first 5

Professional Memberships 3

日本表面科学会
日本分析化学会
日本化学会

Research Interests 3

SNPs
DNA
Analytical reagents

Research Areas 2

Nanotechnology/Materials / Functional solid-state chemistry /
Nanotechnology/Materials / Analytical chemistry /

Awards 6

東北分析化学賞

2012/12　日本分析化学会東北支部
平成23年度特別研究員等審査会専門委員表彰

2012/08　日本学術振興会
Hot Article Award Analytical Sciences

2008/06/10　Analytical Sciences
平成１９年度科学技術分野の文部科学大臣表彰若手科学者賞

2007/04/17　文部科学省　新しいDNA結合試薬に基づく遺伝子検出法の研究
2002年度日本分析化学会奨励賞

2002/09/20　日本分析化学会　分子複合体形成に基づく分析試薬の開発と液液界面イオン認識
第49回日本化学会進歩賞

2000/03/28　日本化学会　水素結合性アニオン認識試薬の開発とその展開

Show all ︎Show 5

Papers 130

Viral Membrane-Targeting Amphipathic Helical Peptide-Based Fluorogenic Probes for the Analysis of Infectious Titers of Enveloped Viruses. International-journal

Yusuke Sato, Yusaku Hatanaka, Yoshitaka Sato, Kota Matsumoto, Shion Osana, Ryoichi Nagatomi, Seiichi Nishizawa

Analytical chemistry　2025/01/22

DOI： 10.1021/acs.analchem.4c04852 　

More details Close

Enveloped viruses have caused the majority of epidemics and pandemics over the past decade. Direct sensing of virus particles (virions) holds great potential for the functional analysis of enveloped viruses. Here, we explore a series of viral membrane-targeting amphipathic helical (AH) peptide-based molecular probes for the assessment of infectious titers of the human coronavirus 229E virus (HCoV-229E). The M2-protein-derived AH peptide is identified as a strong binder for HCoV-229E, and its conjugate with Nile Red, M2-NR, exhibits fluorogenic response upon selective binding to the viral membrane of HCoV-229E. We demonstrate that the response of M2-NR toward the HCoV-229E virus enables the rapid, simple, and reliable assessment of the infectivity of HCoV-229E. In addition, the present fluorescence assay for infectivity analysis is applicable to various kinds of enveloped virus including influenza A virus, herpes simplex virus-1, and lentivirus.
Immobilization of Liposomes into Porous Anodized Aluminum Oxide for On-Demand Release by Ultrasonic Irradiation: Implications for Drug Delivery

Masahiro Okada, Yusuke Sato, Toshihiko Ogura, Tetsuji Itoh, Seiichi Nishizawa

ACS Applied Nano Materials　2024/07/17
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acsanm.4c03267 　

ISSN： 2574-0970

eISSN： 2574-0970
Cationic Oligopeptides with Amino Groups as Synthetic Nucleolar Localization Signals for the Rational Design of Nucleolus-Staining Probes

Michiyuki Suzuki, Yusuke Sato, Nao Togashi, Seiichi Nishizawa

ACS Omega　8　(10)　9592-9596　2023/02/28
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acsomega.3c00116 　

ISSN： 2470-1343

eISSN： 2470-1343
Self-Assembly and Disassembly of Membrane Curvature-Sensing Peptide-Based Deep-Red Fluorescent Probe for Highly Sensitive Sensing of Exosomes

Kaito Ohira, Yusuke Sato, Seiichi Nishizawa

ACS Sensors　8　(2)　522-526　2023/01/25
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acssensors.2c02498 　

ISSN： 2379-3694

eISSN： 2379-3694
Classical thiazole orange and its regioisomer as fluorogenic probes for nucleolar RNA imaging in living cells

Mengmeng He, Yusuke Sato, Seiichi Nishizawa

The Analyst　148　(3)　636-642　2023
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d2an01804g 　

ISSN： 0003-2654

eISSN： 1364-5528

More details Close

Thiazole orange (TO) performs as a promising fluorogenic dye for nucleolar RNA imaging in living cells. More interestingly, 2TO, a regioisomer of TO, performs much better and has superior selectivity for RNA in both solution and living cells.
Kinetic analysis of highly effective triplex formation between a small molecule–peptide nucleic acid conjugate probe and the influenza A virus RNA promoter region at neutral pH

Chioma Uche Okeke, Hiromasa Miura, Yusuke Sato, Seiichi Nishizawa

Organic & Biomolecular Chemistry　21　(16)　3402-3410　2023
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d3ob00262d 　

ISSN： 1477-0520

eISSN： 1477-0539

More details Close

The binding of PNA–small molecule conjugate probes to IAV RNA was examined by means of a stopped-flow technique. It is highly likely that conjugation is the key factor to realize the effective triplex formation of PNA with natural nucleobases at neutral pH.
Bright and Light-Up Sensing of Benzo[c,d]indole-oxazolopyridine Cyanine Dye for RNA and Its Application to Highly Sensitive Imaging of Nucleolar RNA in Living Cells

Kei Higuchi, Yusuke Sato, Nao Togashi, Michiyuki Suzuki, Yukina Yoshino, Seiichi Nishizawa

ACS Omega　7　(27)　23744-23748　2022/06/29
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acsomega.2c02408 　

ISSN： 2470-1343

eISSN： 2470-1343
Fluorescence Sensing of the Panhandle Structure of the Influenza A Virus RNA Promoter by Thiazole Orange Base Surrogate-Carrying Peptide Nucleic Acid Conjugated with Small Molecule. International-journal

Yusuke Sato, Hiromasa Miura, Takaaki Tanabe, Chioma Uche Okeke, Akiko Kikuchi, Seiichi Nishizawa

Analytical chemistry　94　(22)　7814-7822　2022/06/07

DOI： 10.1021/acs.analchem.1c05488 　

More details Close

We have developed a new class of triplex-forming peptide nucleic acid (PNA)-based fluorogenic probes for sensing of the panhandle structure of the influenza A virus (IAV) RNA promoter region. Here, a small molecule (DPQ) capable of selectively binding to the internal loop structure was conjugated with triplex-forming forced intercalation of the thiazole orange (tFIT) probe with natural PNA nucleobases. The resulting conjugate, tFIT-DPQ, showed a significant light-up response (83-fold) upon strong (Kd = 107 nM) and structure-selective binding to the IAV RNA promoter region under physiological conditions (pH 7.0, 100 mM NaCl). We demonstrated the conjugation of these two units through the suitable spacer was key to show useful binding and fluorogenic signaling functions. tFIT-DPQ facilitated the sensitive and selective detection of IAV RNA based on its binding to the promoter region. Furthermore, we found that tFIT-DPQ could work as a sensitive indicator for screening of test compounds targeting the IAV RNA promoter region in the fluorescence indicator displacement assay.
Triplex-Forming Peptide Nucleic Acid Probes Having Cyanine Base Surrogates for Fluorogenic Sensing of Double-Stranded RNA

Seiichi NISHIZAWA, Takaya SATO, En Ting Tabitha LEE, Naonari SAKAMOTO, Toshiki CHIBA, Takaaki TANABE, Yukina YOSHINO, Yuki TAKAHASHI, Yusuke SATO

BUNSEKI KAGAKU　71　(3)　133-144　2022/03/05
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.71.133 　

ISSN： 0525-1931
Molecular Design of Fluorogenic Probes for Targeting rRNA: Indicator in FID Assay and Dye for Imaging of Nucleolar RNA in Living Cells

Seiichi NISHIZAWA, En Ting Tabitha LEE, Yukina YOSHINO, Sayaka YAJIMA, Masafumi ROKUGAWA, Yusuke SATO

BUNSEKI KAGAKU　70　(12)　703-714　2021/12/05
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.70.703 　

ISSN： 0525-1931
Spectroscopic, thermodynamic and kinetic analysis of selective triplex formation by peptide nucleic acid with double‐stranded <scp>RNA</scp> over its <scp>DNA</scp> counterpart

Takaya Sato, Yusuke Sato, Seiichi Nishizawa

Biopolymers　113　(1)　2021/09/03
Publisher: Wiley
DOI： 10.1002/bip.23474 　

ISSN： 0006-3525

eISSN： 1097-0282

More details Close

Abstract Unlike conventional triplex‐forming oligonucleotide (TFO), triplex‐forming peptide nucleic acid (PNA) can tightly bind with double‐stranded RNA (dsRNA) than double‐stranded DNA (dsDNA). Here, we performed spectroscopic, thermodynamic and kinetic experiments for triplex formation by PNA to examine different binding behaviors between PNA − dsRNA and PNA − dsDNA triplexes. We found 9‐mer PNA (cytosine content of 66%) formed the thermally stable triplex with dsRNA compared to dsDNA over a wide range of pH (5.5‐8.0), salt concentration (50‐500 mM NaCl). Both the calorimetric binding constant and the association rate constant for dsRNA were larger than those for dsDNA, indicating the favorable association process for the PNA − dsRNA triplex formation. Comparison with the DNA/RNA heteroduplexes revealed that the DNA strand was detrimental to the triplex stability for PNA, a contrasting result for conventional TFO. The keys underlying the difference in the triplex formation of PNA with different duplexes appear to be the conformational adoptability and the geometric compatibility of PNA to fit the deep, narrow major groove of dsRNA and the helical rigidity difference of the duplexes. Our results emphasize the importance of both the sugar puckering of the duplex and the appropriate conformational flexibility of PNA for the triplex formation.
Fluorogenic Indicator for Targeting HIV-1 TAR RNA: Evaluation for Binging and Signaling Functions of Monomethine Cyanine Dye Thiazole Orange and Its Application to FID Assay

Yoshiko ITO, Yusuke SATO, Norio TERAMAE, Seiichi NISHIZAWA

BUNSEKI KAGAKU　70　(3)　149-157　2021/03/05
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.70.149 　

ISSN： 0525-1931
Deep-red fluorogenic cyanine dyes carrying an amino group-terminated side chain for improved RNA detection and nucleolar RNA imaging

Yusuke Sato, Yugo Igarashi, Michiyuki Suzuki, Kei Higuchi, Seiichi Nishizawa

RSC Advances　11　(56)　35436-35439　2021
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d1ra05872j 　

eISSN： 2046-2069

More details Close

The introduction of an amino-group-terminated side chain into deep-red emissive benzo[c,d]indole–quinoline monomethine cyanine dye has led to the improved detection of RNAs as well as the imaging of nucleolar RNAs in cells.
Chloro-Substituted Naphthyridine Derivative and Its Conjugate with Thiazole Orange for Highly Selective Fluorescence Sensing of an Orphan Cytosine in the AP Site-Containing Duplexes

Chun-xia Wang, Yusuke Sato, Takashi Sugimoto, Norio Teramae, Seiichi Nishizawa

Applied Sciences　10　(12)　4133-4133　2020/06/16
Publisher: MDPI AG
DOI： 10.3390/app10124133 　

eISSN： 2076-3417

More details Close

Fluorescent probes with the binding selectivity to specific structures in DNAs or RNAs have gained much attention as useful tools for the study of nucleic acid functions. Here, chloro-substituted 2-amino-5,7-dimethyl-1,8-naphthyridine (ClNaph) was developed as a strong and highly selective binder for target orphan cytosine opposite an abasic (AP) site in the DNA duplexes. ClNaph was then conjugated with thiazole orange (TO) via an alkyl spacer (ClNaph–TO) to design a light-up probe for the detection of cytosine-related mutations in target DNA. In addition, we found the useful binding and fluorescence signaling of the ClNaph–TO conjugate to target C in AP site-containing DNA/RNA hybrid duplexes with a view toward sequence analysis of microRNAs.
Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA

Yusuke Sato, Yuki Takahashi, Takaaki Tanabe, Seiichi Nishizawa

Organic & Biomolecular Chemistry　18　(21)　4009-4013　2020
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d0ob00794c 　

ISSN： 1477-0520

eISSN： 1477-0539

More details Close

PNA-based fluorescent probes conjugated with the pyrene unit exhibited the improved detection selectivity for double-stranded siRNA.
Amphipathic helical peptide-based fluorogenic probes for a marker-free analysis of exosomes based on membrane-curvature sensing

Yusuke Sato, Kazuki Kuwahara, Kenta Mogami, Kenta Takahashi, Seiichi Nishizawa

RSC Advances　10　(63)　38323-38327　2020
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d0ra07763a 　

eISSN： 2046-2069

More details Close

Fluorogenic probes based on membrane curvature sensing-amphipathic helical peptides have been developed for a marker-free exosome analysis.
Small molecule–PNA oligomer conjugates for rRNA A-site at neutral pH for FID assays

En Ting Tabitha Lee, Yusuke Sato, Seiichi Nishizawa

Chemical Communications　56　(95)　14976-14979　2020
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d0cc06084d 　

ISSN： 1359-7345

eISSN： 1364-548X

More details Close

A conjugation strategy was used to synthesize light-up probes for the bacterial rRNA A-site with strong binding affinity and selectivity.
Strong Binding and Off–On Signaling Functions of Deep-Red Fluorescent TO-PRO-3 for Influenza A Virus RNA Promoter Region

Yusuke Sato, Yuri Aiba, Sayaka Yajima, Takaaki Tanabe, Kei Higuchi, Seiichi Nishizawa

ChemBioChem　20　(21)　2752-2756　2019/11/04
Publisher: Wiley-VCH Verlag
DOI： 10.1002/cbic.201900331 　

ISSN： 1439-7633 1439-4227
Deep-Red Light-up Signaling of Benzo[c,d]indole–Quinoline Monomethine Cyanine for Imaging of Nucleolar RNA in Living Cells and for Sequence-Selective RNA Analysis

Yukina Yoshino, Yusuke Sato, Seiichi Nishizawa

Analytical Chemistry　91　(22)　14254-14260　2019/10/09
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.9b01997 　

ISSN： 0003-2700

eISSN： 1520-6882
Programmable RNA detection with a fluorescent RNA aptamer using optimized three-way junction formation. International-journal

Yuichi Furuhata, Mizuki Kobayashi, Ryo Maruyama, Yusuke Sato, Kurumi Makino, Tatsuo Michiue, Hiroharu Yui, Seiichi Nishizawa, Keitaro Yoshimoto

RNA (New York, N.Y.)　25　(5)　590-599　2019/05

DOI： 10.1261/rna.069062.118 　

More details Close

RNAs play essential roles in various cellular processes and can be used as biomarkers. Hence, it is important to detect endogenous RNA for understanding diverse cellular functions and diagnosing diseases. To construct a low-cost and easy-to-use RNA detection probe, a chemically unmodified RNA aptamer that binds to a pro-fluorophore to increase its fluorescence is desirable. Here, we focused on Broccoli, a superior variant of Spinach, which is a well-known fluorescent RNA aptamer that binds to DFHBI-1T and emits green fluorescence. We experimentally characterized Broccoli and predicted that it forms a G-quadruplex-based DFHBI-1T recognition region sandwiched between two stems. Based on this, we designed a Broccoli-based RNA detection probe (BRD probe) composed of a sequence of destabilized Broccoli fused with complementary sequences against target RNA. The resulting probe with its target RNA formed a stable three-way junction, named the MT2 three-way junction, which contributed to efficient refolding of the Broccoli structure and allowed for programmable RNA detection with high signal-to-noise ratio and sensitivity. Interestingly, the MT2 three-way junction also could be applied to probe construction of a truncated form of Spinach (Baby Spinach). The BRD and Baby Spinach-based RNA detection probes (BSRD probe) exhibited up to 48- and 140-fold fluorescence enhancements in the presence of their target RNAs and detected small amounts of target RNA that were as low as 160 and 5 nM, respectively. Thus, we experimentally characterized the higher order structure of Broccoli and developed structure-switching aptamer probes for highly sensitive, programmable, RNA detection using an MT2 three-way junction.
Enhanced Binding Affinity of siRNA Overhang-Binding Fluorescent Probes by Conjugation with Cationic Oligopeptides for Improved Analysis of the siRNA Delivery Process

Yusuke Sato, Mitsumasa Kaneko, Takaya Sato, Saki Nakata, Yuki Takahashi, Seiichi Nishizawa

ChemBioChem　20　(3)　408-414　2019/02/01
Publisher: Wiley-VCH Verlag
DOI： 10.1002/cbic.201800560 　

ISSN： 1439-7633 1439-4227
Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA

Yusuke Sato, Sayaka Yajima, Akifumi Taguchi, Kyosuke Baba, Mayu Nakagomi, Yuri Aiba, Seiichi Nishizawa

Chemical Communications　55　(22)　3183-3186　2019
Publisher: Royal Society of Chemistry
DOI： 10.1039/c9cc00414a 　

ISSN： 1364-548X 1359-7345
Fluorescent Trimethylated Naphthyridine Derivative with an Aminoalkyl Side Chain as the Tightest Non-aminoglycoside Ligand for the Bacterial A-site RNA

Yusuke Sato, Masafumi Rokugawa, Sho Ito, Sayaka Yajima, Hiroki Sugawara, Norio Teramae, Seiichi Nishizawa

Chemistry - A European Journal　24　(52)　13862-13870　2018/09/18
Publisher: Wiley-VCH Verlag
DOI： 10.1002/chem.201802320 　

ISSN： 1521-3765 0947-6539
Effect of Cavity Size of Mesoporous Silica on Short DNA Duplex Stability

Tsubasa Masuda, Yuuta Shibuya, Shota Arai, Sayaka Kobayashi, Sotaro Suzuki, Jun Kijima, Tetsuji Itoh, Yusuke Sato, Seiichi Nishizawa, Akira Yamaguchi

LANGMUIR　34　(19)　5545-5550　2018/05

DOI： 10.1021/acs.langmuir.8b00437 　

ISSN： 0743-7463
Highly sensitive programmable RNA detection using fluorescent RNA aptamer Peer-reviewed

Furuhata Yuichi, Kobayahshi Mizuki, Maruyama Ryo, Sato Yusuke, Makino Kurumi, Michiue Tatsuo, Yui Hiroharu, Nishizawa Seiichi, Yoshimoto Keitaro

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　255　2018/03/18

ISSN： 0065-7727
Design and synthesis of RNA-binding fluorescent probes for analysis of small RNAs

Yusuke Sato, Takaya Sato, Seiichi Nishizawa

Bunseki Kagaku　67　(9)　531-540　2018
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.67.531 　

ISSN： 0525-1931
Design of a fluorogenic PNA probe capable of simultaneous recognition of 3′-overhang and double-stranded sequences of small interfering RNAs

Takaaki Tanabe, Takaya Sato, Yusuke Sato, Seiichi Nishizawa

RSC Advances　8　(73)　42095-42099　2018
Publisher: Royal Society of Chemistry
DOI： 10.1039/c8ra08759h 　

ISSN： 2046-2069
Improved boronate affinity electrophoresis by optimization of the running buffer for a single-step separation of piRNA from mouse testis total RNA Peer-reviewed

Yusuke Sato, Daijiro Iwasawa, Kuo Ping Hui, Rena Nakagomi, Seiichi Nishizawa

Analytical Sciences　34　(5)　627-630　2018
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/analsci.17N024 　

ISSN： 1348-2246 0910-6340
Red-emissive triplex-forming PNA probes carrying cyanine base surrogates for fluorescence sensing of double-stranded RNA Peer-reviewed

Toshiki Chiba, Takaya Sato, Yusuke Sato, Seiichi Nishizawa

ORGANIC & BIOMOLECULAR CHEMISTRY　15　(37)　7765-7769　2017/10

DOI： 10.1039/c7ob02077e 　

ISSN： 1477-0520

eISSN： 1477-0539
Optimization of the Alkyl Linker of TO Base Surrogate in Triplex-Forming PNA for Enhanced Binding to Double-Stranded RNA Peer-reviewed

Takaya Sato, Yusuke Sato, Seiichi Nishizawa

CHEMISTRY-A EUROPEAN JOURNAL　23　(17)　4079-4088　2017/03

DOI： 10.1002/chem.201604676 　

ISSN： 0947-6539

eISSN： 1521-3765
Pteridine Derivatives Modified with a Guanidine for Binding and Sensing an Orphan Guanine in RNA Duplexes Peer-reviewed

Yusuke Sato, Takahiro Asami, Yu Toriyabe, Takaya Sato, Norio Teramae, Seiichi Nishizawa

CHEMISTRY LETTERS　45　(8)　982-984　2016/08

DOI： 10.1246/cl.160425 　

ISSN： 0366-7022

eISSN： 1348-0715
Triplex-Forming Peptide Nucleic Acid Probe Having Thiazole Orange as a Base Surrogate for Fluorescence Sensing of Double-stranded RNA Peer-reviewed

Takaya Sato, Yusuke Sato, Seiichi Nishizawa

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　138　(30)　9397-9400　2016/08

DOI： 10.1021/jacs.6b05554 　

ISSN： 0002-7863
Thermodynamics of Complexation between Thiourea-based Receptor and Acetate in Water/Acetonitrile Mixture Peer-reviewed

Takaya Suzuki, Yuuta Shibuya, Takaya Sato, Seiichi Nishizawa, Itaru Sato, Akira Yamaguchi

ANALYTICAL SCIENCES　32　(7)　741-744　2016/07

DOI： 10.2116/analsci.31.315 　

ISSN： 0910-6340

eISSN： 1348-2246
Lysine linkage in abasic site-binding ligand-thiazole orange conjugates for improved binding affinity to orphan nucleobases in DNA/RNA hybrids Peer-reviewed

Yusuke Sato, Hiroki Saito, Daisuke Aoki, Norio Teramae, Seiichi Nishizawa

CHEMICAL COMMUNICATIONS　52　(100)　14446-14449　2016

DOI： 10.1039/c6cc07236d 　

ISSN： 1359-7345

eISSN： 1364-548X
Fluorescence Imaging of siRNA Delivery by Peptide Nucleic Acid-based Probe Peer-reviewed

Takaya Sato, Yusuke Sato, Kenta Iwai, Shusuke Kuge, Norio Teramae, Seiichi Nishizawa

ANALYTICAL SCIENCES　31　(4)　315-320　2015/04

DOI： 10.2116/analsci.31.315 　

ISSN： 0910-6340

eISSN： 1348-2246
A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity Peer-reviewed

Jing Tao, Panshu Song, Yusuke Sato, Seiichi Nishizawa, Norio Teramae, Aijun Tong, Yu Xiang

CHEMICAL COMMUNICATIONS　51　(5)　929-932　2015

DOI： 10.1039/c4cc06170e 　

ISSN： 1359-7345

eISSN： 1364-548X
Synthetic fluorescent probes capable of selective recognition of 3 '-overhanging nucleotides for siRNA delivery imaging Peer-reviewed

Takaya Sato, Yusuke Sato, Kenta Iwai, Shusuke Kuge, Seiichi Nishizawa, Norio Teramae

CHEMICAL COMMUNICATIONS　51　(8)　1421-1424　2015

DOI： 10.1039/c4cc08800j 　

ISSN： 1359-7345

eISSN： 1364-548X
Abasic site-binding ligands conjugated with cyanine dyes for "off-on" fluorescence sensing of orphan nucleobases in DNA duplexes and DNA-RNA hybrids Peer-reviewed

Yusuke Sato, Megumi Kudo, Yu Toriyabe, Shota Kuchitsu, Chun-Xia Wang, Seiichi Nishizawa, Norio Teramae

CHEMICAL COMMUNICATIONS　50　(5)　515-517　2014

DOI： 10.1039/c3cc47717g 　

ISSN： 1359-7345

eISSN： 1364-548X
Recent Progress in Abasic Site-binding Small Molecules for Detecting Single-base Mutations in DNA Invited Peer-reviewed

Seiichi Nishizawa, Yusuke Sato, Norio Teramae

ANALYTICAL SCIENCES　30　(1)　137-142　2014/01

DOI： 10.2116/analsci.30.137 　

ISSN： 0910-6340

eISSN： 1348-2246
The effect of LNA nucleobases as enhancers for the binding of amiloride to an abasic site in DNA/DNA and DNA/RNA duplexes Peer-reviewed

Yusuke Sato, Tetsushi Sato, Takaya Sato, Seiichi Nishizawa, Norio Teramae

ORGANIC & BIOMOLECULAR CHEMISTRY　12　(37)　7250-7256　2014

DOI： 10.1039/c4ob00977k 　

ISSN： 1477-0520

eISSN： 1477-0539
Rational Design for Cooperative Recognition of Specific Nucleobases Using -Cyclodextrin-Modified DNAs and Fluorescent Ligands on DNA and RNA Scaffolds Peer-reviewed

Futamura, Akika, Uemura, Asuka, Imoto, Takeshi, Kitamura, Yusuke, Matsuura, Hirotaka, Wang, Chun-Xia, Ichihashi, Toshiki, Sato, Yusuke, Teramae, Norio, Nishizawa, Seiichi, Ihara, Toshihiro

CHEMISTRY-A EUROPEAN JOURNAL　19　(32)　10526-10535　2013/08

DOI： 10.1002/chem.201300985 　

ISSN： 0947-6539

eISSN： 1521-3765
Competitive Binding of Abasic Site-Binding Ligands and Masking Ligands to DNA Duplexes for the Analysis of Single-Base Mutation Peer-reviewed

Yusuke Sato, Tomoe Kageyama, Seiichi Nishizawa, Norio Teramae

ANALYTICAL SCIENCES　29　(1)　15-19　2013/01

DOI： 10.2116/analsci.29.15 　

ISSN： 0910-6340
2,4-Diamino-6,7-dimethylpteridine as a fluorescent ligand for binding and sensing an orphan cytosine in RNA duplexes Peer-reviewed

Yusuke Sato, Yu Toriyabe, Seiichi Nishizawa, Norio Teramae

CHEMICAL COMMUNICATIONS　49　(85)　9983-9985　2013

DOI： 10.1039/c3cc46085a 　

ISSN： 1359-7345
Competitive Assay for Theophylline Based on an Abasic Site-Containing DNA Duplex Aptamer and a Fluorescent Ligand Peer-reviewed

Yusuke Sato, Yushuang Zhang, Seiichi Nishizawa, Takehiro Seino, Kodai Nakamura, Minjie Li, Norio Teramae

CHEMISTRY-A EUROPEAN JOURNAL　18　(40)　12719-12724　2012/10

DOI： 10.1002/chem.201201298 　

ISSN： 0947-6539
Ratiometric Fluorescent Signaling of Small Molecule, Environmentally Sensitive Dye Conjugates for Detecting Single-Base Mutations in DNA Peer-reviewed

Chun-xia Wang, Yusuke Sato, Megumi Kudo, Seiichi Nishizawa, Norio Teramae

CHEMISTRY-A EUROPEAN JOURNAL　18　(31)　9481-9484　2012/07

DOI： 10.1002/chem.201200219 　

ISSN： 0947-6539
Base Pairing at the Abasic Site in DNA Duplexes and Its Application in Adenosine Aptasensors Peer-reviewed

Yuanfeng Pang, Zhiai Xu, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

CHEMBIOCHEM　13　(3)　436-442　2012/02

DOI： 10.1002/cbic.201100666 　

ISSN： 1439-4227
Highly selective binding of naphthyridine with a trifluoromethyl group to cytosine opposite an abasic site in DNA duplexes Peer-reviewed

Yusuke Sato, Yushuang Zhang, Takehiro Seino, Takashi Sugimoto, Seiichi Nishizawa, Norio Teramae

ORGANIC & BIOMOLECULAR CHEMISTRY　10　(20)　4003-4006　2012

DOI： 10.1039/c2ob25513h 　

ISSN： 1477-0520
Strong and Selective Binding of Amiloride to an Abasic Site in RNA Duplexes: Thermodynamic Characterization and MicroRNA Detection Peer-reviewed

Yusuke Sato, Toshiki Ichihashi, Seiichi Nishizawa, Norio Teramae

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　51　(26)　6369-6372　2012

DOI： 10.1002/anie.201201790 　

ISSN： 1433-7851
DNA-Binding Small-Ligand-Immobilized Surface Plasmon Resonance Biosensor for Detecting Thymine-Related Single-Nucleotide Polymorphisms Peer-reviewed

Sara Miura, Seiichi Nishizawa, Akinori Suzuki, Yukiko Fujimoto, Katsuya Ono, Qiang Gao, Norio Teramae

CHEMISTRY-A EUROPEAN JOURNAL　17　(50)　14104-14110　2011/12

DOI： 10.1002/chem.201101290 　

ISSN： 0947-6539
Label-Free Molecular Beacon System Based on DNAs Containing Abasic Sites and Fluorescent Ligands That Bind Abasic Sites Peer-reviewed

Yusuke Sato, Seiichi Nishizawa, Norio Teramae

CHEMISTRY-A EUROPEAN JOURNAL　17　(41)　11650-11656　2011/10

DOI： 10.1002/chem.201100384 　

ISSN： 0947-6539
Fluorescent aptasensors based on conformational adaptability of abasic site-containing aptamers in combination with abasic site-binding ligands Peer-reviewed

Zhiai Xu, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

BIOSENSORS & BIOELECTRONICS　26　(12)　4733-4738　2011/08

DOI： 10.1016/j.bios.2011.05.051 　

ISSN： 0956-5663
Fluorescent trimethyl-substituted naphthyridine as a ligand for C-C mismatch detection in CCG trinucleotide repeats Peer-reviewed

Yusuke Sato, Atsuko Honjo, Daisuke Ishikawa, Seiichi Nishizawa, Norio Teramae

CHEMICAL COMMUNICATIONS　47　(20)　5885-5887　2011

DOI： 10.1039/c1cc11370d 　

ISSN： 1359-7345
Enhancement in fluorescence response by a quencher for amiloride upon binding to thymine opposite an abasic site in a DNA duplex Peer-reviewed

Yusuke Sato, Jing Tian, Toshiki Ichihashi, Yuka Chinda, Zhiai Xu, Yuanfeng Pang, Seiichi Nishizawa, Norio Teramae

ANALYTICA CHIMICA ACTA　675　(1)　49-52　2010/08

DOI： 10.1016/j.aca.2010.06.042 　

ISSN： 0003-2670
Effect of the bases flanking an abasic site on the recognition of nucleobase by amiloride Peer-reviewed

Arivazhagan Rajendran, Chunxia Zhao, Burki Rajendar, Viruthachalam Thiagarajan, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS　1800　(6)　599-610　2010/06

DOI： 10.1016/j.bbagen.2010.03.007 　

ISSN： 0304-4165
NBD-Based Green Fluorescent Ligands for Typing of Thymine-Related SNPs by Using an Abasic Site-Containing Probe DNA Peer-reviewed

Viruthachalam Thiagarajan, Arivazhagan Rajendran, Hiroyuki Satake, Seiichi Nishizawa, Norio Teramae

CHEMBIOCHEM　11　(1)　94-100　2010/01

DOI： 10.1002/cbic.200900530 　

ISSN： 1439-4227
Abasic site-based DNA aptamers for analytical applications Peer-reviewed

Seiichi Nishizawa, Yusuke Sato, Zhiai Xu, Kotaro Morita, Minjie Li, Norio Teramae

SUPRAMOLECULAR CHEMISTRY　22　(7-8)　467-476　2010

DOI： 10.1080/10610278.2010.484865 　

ISSN： 1061-0278
Effect of substituents of alloxazine derivatives on the selectivity and affinity for adenine in AP-site-containing DNA duplexes Peer-reviewed

Burki Rajendar, Arivazhagan Rajendran, Zhiqiang Ye, Eriko Kanai, Yusuke Sato, Seiichi Nishizawa, Marek Sikorski, Norio Teramae

ORGANIC & BIOMOLECULAR CHEMISTRY　8　(21)　4949-4959　2010

DOI： 10.1039/c0ob00057d 　

ISSN： 1477-0520
Influence of substituent modifications on the binding of 2-amino-1,8-naphthyridines to cytosine opposite an AP site in DNA duplexes: thermodynamic characterization Peer-reviewed

Yusuke Sato, Seiichi Nishizawa, Keitaro Yoshimoto, Takehiro Seino, Toshiki Ichihashi, Kotaro Morita, Norio Teramae

NUCLEIC ACIDS RESEARCH　37　(5)　1411-1422　2009/04

DOI： 10.1093/nar/gkn1079 　

ISSN： 0305-1048

eISSN： 1362-4962
Small-Molecule Binding at an Abasic Site of DNA: Strong Binding of Lumiflavin for Improved Recognition of Thymine-Related Single Nucleotide Polymorphisms Peer-reviewed

N. B. Sankaran, Yusuke Sato, Fuyuki Sato, Burki Rajendar, Kotaro Morita, Takehiro Seino, Seiichi Nishizawa, Norio Teramae

JOURNAL OF PHYSICAL CHEMISTRY B　113　(5)　1522-1529　2009/02

DOI： 10.1021/jp808576t 　

ISSN： 1520-6106
Simultaneous recognition of nucleobase and sites of DNA damage: Effect of tethered cation on the binding affinity Peer-reviewed

Arivazhagan Rajendran, Viruthachalam Thiagarajan, Burki Rajendar, Seiichi Nishizawa, Norio Teramae

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS　1790　(2)　95-100　2009/02

DOI： 10.1016/j.bbagen.2008.09.003 　

ISSN： 0304-4165
Cooperative DNA Probing Using a beta-Cyclodextrin-DNA Conjugate and a Nucleobase-Specific Fluorescent Ligand Peer-reviewed

Toshihiro Ihara, Asuka Uemura, Akika Futamura, Masamichi Shimizu, Noriyuki Baba, Seiichi Nishizawa, Norio Teramae, Akinori Jyo

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　131　(4)　1386-+　2009/02

DOI： 10.1021/ja809023n 　

ISSN： 0002-7863
2-Aminopurine-Modified Abasic-Site-Containing Duplex DNA for Highly Selective Detection of Theophylline Peer-reviewed

Minjie Li, Yusuke Sato, Seiichi Nishizawa, Takehiro Seino, Kodai Nakamura, Norio Teramae

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　131　(7)　2448-+　2009/02

DOI： 10.1021/ja8095625 　

ISSN： 0002-7863
Effect of methyl substitution in a ligand on the selectivity and binding affinity for a nucleobase: A case study with isoxanthopterin and its derivatives Peer-reviewed

Burki Rajendar, Arivazhagan Rajendran, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

BIOORGANIC & MEDICINAL CHEMISTRY　17　(1)　351-359　2009/01

DOI： 10.1016/j.bmc.2008.10.062 　

ISSN： 0968-0896
Signal-Off and Signal-On Design for a Label-Free Aptasensor Based on Target-Induced Self-Assembly and Abasic-Site-Binding Ligands Peer-reviewed

Zhiai Xu, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

CHEMISTRY-A EUROPEAN JOURNAL　15　(40)　10375-10378　2009

DOI： 10.1002/chem.200901226 　

ISSN： 0947-6539
Label-free aptamer-based sensor using abasic site-containing DNA and a nucleobase-specific fluorescent ligand Peer-reviewed

Zhiai Xu, Kotaro Morita, Yusuke Sato, Qing Dai, Seiichi Nishizawa, Norio Teramae

CHEMICAL COMMUNICATIONS　(42)　6445-6447　2009

DOI： 10.1039/b908345f 　

ISSN： 1359-7345
A surface plasmon resonance sensor based on 3,5-diaminopyrazine with a high selectivity for thymine in AP site-containing DNA duplex

Sara Miura, Katsuya Ono, Miwa Watanabe, Seiichi Nishizawa, Norio Teramae

Nucleic Acids Symp. Ser.　52　123-124　2008/09/08

DOI： 10.1093/nass/nrn063 　
Development of label-free molecular beacons based on abasic site-binding fluorescence molecules

Yusuke Sato, Seiichi Nishizawa, Norio Teramae

Nucleic Acids Symp. Ser.　52　121-122　2008/09/08

DOI： 10.1093/nass/nrn062 　
Competitive binding of small ligands to nucleobases in AP site-containing DNA duplexes

Tomoe Kageyama, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

Nucleic Acids Symp. Ser.　52　119-120　2008/09/08

DOI： 10.1093/nass/nrn061 　
Effect of an alkyl amino group on the binding of 1,8-naphthyridines to AP site-containing DNA duplexes

Toshiki Ichihashi, Yusuke Sato, Takehiro Seino, Seiichi Nishizawa, Norio Teramae

Nucleic Acids Symp. Ser.　52　117-118　2008/09/08

DOI： 10.1093/nass/nrn060 　
A pteridine derivative with electron-withdrawing groups for binding and sensing of nucleobases in AP site-containing DNA duplexes

Eriko Kanai, Seiichi Nishizawa, Norio Teramae

Nucleic Acids Symp. Ser.　52　115-116　2008/09/08

DOI： 10.1093/nass/nrn059 　
A pyrazine-based fluorescence-enhancing ligand with a high selectivity for thymine in AP site-containing DNA duplexes Peer-reviewed

Chunxia Zhao, Arivazhagan Rajendran, Qing Dai, Sefichi Nishizawa, Norio Teramae

ANALYTICAL SCIENCES　24　(6)　693-695　2008/06

DOI： 10.2116/analsci.24.693 　

ISSN： 0910-6340
Sequence dependence of cytochrome c electrochemistry on DNA modified electrodes: Effect of hydrogen bonding of a ligand to nucleobases opposite an abasic site Peer-reviewed

Yong Shao, Kotaro Morita, Qing Dai, Seiichi Nishizawa, Norio Teramae

ELECTROCHEMISTRY COMMUNICATIONS　10　(3)　438-442　2008/03

DOI： 10.1016/j.elecom.2008.01.008 　

ISSN： 1388-2481
Fluorescence and electrochemical detection of pyrimidine/purine transversion by a ferrocenyl aminonaphthyridine derivative Peer-reviewed

Kotaro Morita, Yusuke Sato, Takehiro Seino, Seiichi Nishizawa, Norio Teramae

ORGANIC & BIOMOLECULAR CHEMISTRY　6　(2)　266-268　2008

DOI： 10.1039/b716682f 　

ISSN： 1477-0520
Alloxazine as a ligand for selective binding to adenine opposite AP sites in DNA duplexes and analysis of single-nucleotide polymorphisms Peer-reviewed

Burki Rajendar, Seiichi Nishizawa, Norio Teramae

ORGANIC & BIOMOLECULAR CHEMISTRY　6　(4)　670-673　2008

DOI： 10.1039/b719786a 　

ISSN： 1477-0520
6,7-Dimethyllumazine as a potential ligand for selective recognition of adenine opposite an abasic site in DNA duplexes Peer-reviewed

Zhiqiang Ye, Burki Rajendar, Dai Qing, Seiichi Nishizawa, Norio Teramae

CHEMICAL COMMUNICATIONS　2008　(48)　6588-6590　2008

DOI： 10.1039/b816876h 　

ISSN： 1359-7345
Electrochamical and fluorescence detection of cytosine-related SNPs using ferrocenyl naphthyridine derivative

K. Morita, Y. Sato, T. Seino, S. Nishizawa, N. Teramae

Nucleic Acids Symp. Ser.　51　(1)　295-296　2007/11

DOI： 10.1093/nass/nrm148 　
Fluorescence emission detection of single-nucleotide polymorphisms by a naphthyridine-benzofurazan conjugate

H. Satake, S. Nishizawa, N. Teramae

Nucleic Acids Symp. Ser.　51　(1)　297-298　2007/11

DOI： 10.1093/nass/nrm149 　
Strong binding of naphthyridine derivatives to cytosine in an AP site-containing DNA duplex and their application to fluorescence detection of single nucleotide polymorphisms

Y. Sato, T. Seino, S. Nishizawa, N. Teramae

Nucleic Acids Symp. Ser.　51　(1)　313-314　2007/11

DOI： 10.1093/nass/nrm157 　
Improvement of base selectivity and binding affinity by controlling hydrogen bonding motifs between nucleobases and isoxanthopterin: Application to the detection of T/C mutation Peer-reviewed

Burki Rajendar, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　17　(13)　3682-3685　2007/07

DOI： 10.1016/j.bmcl.2007.04.033 　

ISSN： 0960-894X
Fluorescence detection of single-nucleotide polymorphisms with two simple and low cost methods: A double-DNA-probe method and a bulge form method Peer-reviewed

Na Li, Ling Mei, Yu Xiang, Aijun Tong, Seiichi Nishizawa, Norio Teramae

ANALYTICA CHIMICA ACTA　597　(1)　97-102　2007/07

DOI： 10.1016/j.aca.2007.06.031 　

ISSN： 0003-2670
Fluorescent-based SNP typing using hydrogen bond-forming small ligand and its lanthanide(III) complex Peer-reviewed

Keitaxo Yoshimoto, Hiroshi Atsumi, Seiichi Nishizawa, Shingo Saito, Hiroyuki Koshino, Moriya Okuma, Mizuo Maeda, Norio Teramae, Yukio Nagasaki

PROGRESS ON POST-GENOME TECHNOLOGIES　22-23　2007
Electrochemical detection at low temperature for a specific nucleobase of target nucleic acids by an abasic site-containing DNA binding ligand Peer-reviewed

W Huang, K Morita, NB Sankaran, S Nishizawa, N Teramae

ELECTROCHEMISTRY COMMUNICATIONS　8　(3)　395-398　2006/03

DOI： 10.1016/j.elecom.2005.12.021 　

ISSN： 1388-2481
Ratiometric fluorescence detection of pyrimidine/purine transversion by using a 2-amino-1,8-naphthyridine derivative

H. Satake, S. Nishizawa, N. Teramae

Anal. Sci.　22 (2), 195-197　2006/03

DOI： 10.2116/analsci.22.195 　
Enhancement of the binding ability of a ligand for nucleobase recognition by introducing a methyl group Peer-reviewed

Q Dai, CY Xu, Y Sato, K Yoshimoto, S Nishizawa, N Teramae

ANALYTICAL SCIENCES　22　(2)　201-203　2006/02

ISSN： 0910-6340
Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone Peer-reviewed

CX Zhao, Q Dai, T Seino, YY Cui, S Nishizawa, N Teramae

CHEMICAL COMMUNICATIONS　(11)　1185-1187　2006

DOI： 10.1039/b5165756j 　

ISSN： 1359-7345
Use of vitamin B-2 for fluorescence detection of thymidine-related single-nucleotide polymorphisms Peer-reviewed

S Nishizawa, NB Sankaran, T Seino, YY Cui, Q Dai, CY Xu, K Yoshimoto, N Teramae

ANALYTICA CHIMICA ACTA　556　(1)　133-139　2006/01

DOI： 10.1016/j.aca.2005.05.064 　

ISSN： 0003-2670
Electrochemical SNPs detection using an abasic site-containing DNA on a gold electrode Peer-reviewed

Kotaro Morita, N. B. Sankaran, Weimin Huang, Takehiro Seino, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

CHEMICAL COMMUNICATIONS　(22)　2376-2378　2006

DOI： 10.1039/b517391d 　

ISSN： 1359-7345
Abasic-site-containing oligodeoxynucleotides as aptamers for riboflavin Peer-reviewed

NB Sankaran, S Nishizawa, T Seino, K Yoshimoto, N Teramae

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　45　(10)　1563-1568　2006

DOI： 10.1002/anie.200502979 　

ISSN： 1433-7851
Strong binding of naphthyridine derivatives to a guanine base in DNA duplexes containing an AP site

Q. Gao, H. Satake, Q. Dai, K. Ono, S. Nishizawa, N. Teramae

Nucleic Acids Symp. Ser.　49, 219-220　2005/09
Designing ratiometric fluorescent sensors for alkali metal ions from simple PET sensors by controlling spacer length

N. B. Sankaran, Seiichi Nishizawa, Masatoshi Watanabe, Tatsuya Uchida, Norio Teramae

Journal of Materials Chemistry　15　(27-28)　2755-2761　2005/07/21

DOI： 10.1039/b500958h 　

ISSN： 0959-9428
Assignment of hydrogen-bond structure in a ligand-nucleobase complex inside duplex DNA: combined use of quantum chemical calculations and 15N NMR experiments.

Keitaro Yoshimoto, Seiichi Nishizawa, Hiroyuki Koshino, Yusuke Sato, Norio Teramae, Mizuo Maeda

Nucleic acids symposium series (2004)　(49)　255-256　2005

DOI： 10.1093/nass/49.1.255 　

ISSN： 1746-8272
Hydrogen bond-mediated binding of ligands to a nucleobase at a gap site in a DNA duplex and its use for fluorescence detection of single-nucleotide polymorphisms.

Takehiro Seino, Seiichi Nishizawa, Norio Teramae

Nucleic acids symposium series (2004)　(49)　205-206　2005

DOI： 10.1093/nass/49.1.205 　

ISSN： 1746-8272
Fluorescence detection of thymidine-related single-nucleotide polymorphisms by 3, 5-diaminopyrazine derivatives.

Chunxia Zhao, Qing Dai, Takehiro Seino, Ying-Yu Cui, Seiichi Nishizawa, Norio Teramae

Nucleic acids symposium series (2004)　(49)　221-222　2005

DOI： 10.1093/nass/49.1.221 　

ISSN： 1746-8272
Facilitated sulfate transfer across the nitrobenzene-water interface as mediated by hydrogen-bonding ionophores Peer-reviewed

S Nishizawa, T Kamaishi, T Yokobori, R Kato, YY Cui, T Shioya, N Teramae

ANALYTICAL SCIENCES　20　(11)　1559-1565　2004/11

DOI： 10.2116/analsci.20.1559 　

ISSN： 0910-6340
Fluorescence detection of cytosine/guanine transversion based on a hydrogen bond forming ligand Peer-reviewed

S Nishizawa, K Yoshimoto, T Seino, CY Xu, M Minagawa, H Satake, A Tong, N Teramae

TALANTA　63　(1)　175-179　2004/05

DOI： 10.1016/j.talanta.2003.09.027 　

ISSN： 0039-9140
Thiourea-isothiouronium conjugate for strong and selective binding of very hydrophilic H2PO4- anion at the 1,2-dichloroethane-water interface Peer-reviewed

R Kato, YY Cui, S Nishizawa, T Yokobori, N Teramae

TETRAHEDRON LETTERS　45　(22)　4273-4276　2004/05

DOI： 10.1016/j.tetlet.2004.04.001 　

ISSN： 0040-4039
Study of the binding mode of a chromophoric host zinc protoporphyrin with histamine Peer-reviewed

AJ Tong, CY Tong, QY Yang, Y Kato, S Nishizawa, N Teramae

ACTA CHIMICA SINICA　62　(9)　905-910　2004/05

ISSN： 0567-7351
Nucleobase recognition by hydrogen bond forming ligands and its use for fluorescence detection of single-nucleotide polymorphisms Peer-reviewed

S Nishizawa, K Yoshimoto, T Seino, CY Xu, N Teramae

BUNSEKI KAGAKU　53　(5)　383-391　2004/05

DOI： 10.2116/bunsekikagaku.53.383 　

ISSN： 0525-1931
Fluorescence detection of guanine-adenine transition by a hydrogen bond forming small compound Peer-reviewed

K Yoshimoto, CY Xu, S Nishizawa, T Haga, H Satake, NE Teramae

CHEMICAL COMMUNICATIONS　(24)　2960-2961　2003/12

DOI： 10.1039/b309229a 　

ISSN： 1359-7345
Anion recognition at the solid/liquid interface as studied by second harmonic generation spectroscopy Peer-reviewed

A Yamaguchi, R Kato, S Nishizawa, N Teramae

CHEMISTRY LETTERS　32　(9)　798-799　2003/09

DOI： 10.1246/cl.2003.798 　

ISSN： 0366-7022

eISSN： 1348-0715
Use of abasic site-containing DNA strands for nucleobase recognition in water Peer-reviewed

K Yoshimoto, S Nishizawa, M Minagawa, N Teramae

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　125　(30)　8982-8983　2003/07

DOI： 10.1021/ja029786m 　

ISSN： 0002-7863
Hydrogen-bond forming ionophore for highly efficient transport of phosphate anions across the nitrobenzene-water interface Peer-reviewed

S Nishizawa, T Yokobori, R Kato, K Yoshimoto, T Kamaishi, N Teramae

ANALYST　128　(6)　663-669　2003

DOI： 10.1039/b301141k 　

ISSN： 0003-2654
DNA脱塩基部位を利用する核酸塩基認識と一塩基変異蛍光検出

西沢精一, 吉本敬太郎, 寺前紀夫

日本化学会生体機能関連部会 News Letter　18　(2)　6-9　2003
Template-assisted preferential formation of a syn photodimer in a pyrophosphate-induced self-assembly of a thymine-functional ized isothiouronium receptor Peer-reviewed

Y Kato, S Nishizawa, N Teramae

ORGANIC LETTERS　4　(25)　4407-4410　2002/12

DOI： 10.1021/ol0268485 　

ISSN： 1523-7060
Conversion of thioureas to fluorescent isothiouronium-based photoinduced electron transfer sensors for oxoanion sensing Peer-reviewed

S Nishizawa, YY Cui, M Minagawa, K Morita, Y Kato, S Taniguchi, R Kato, N Teramae

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2　(5)　866-870　2002

DOI： 10.1039/b201704k 　

ISSN： 1472-779X
Selective binding of very hydrophilic H2PO4- anion by a hydrogen-bonding receptor adsorbed at the 1,2-dichloroethane-water interface Peer-reviewed

K Shigemori, S Nishizawa, T Yokobori, T Shioya, N Teramae

NEW JOURNAL OF CHEMISTRY　26　(9)　1102-1104　2002

DOI： 10.1039/b203881a 　

ISSN： 1144-0546
Chloride transfer across the liquid-liquid interface facilitated by a mono-thiourea as a hydrogen-bonding ionophore Peer-reviewed

S Nishizawa, T Yokobori, R Kato, T Shioya, N Teramae

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN　74　(12)　2343-2347　2001/12

DOI： 10.1246/bcsj.74.2343 　

ISSN： 0009-2673

eISSN： 1348-0634
Facilitated transfer of hydrophilic anions across the nitrobenzene-water interface by a hydrogen-bonding ionophore: Applicability for multianalyte detection Peer-reviewed

S Nishizawa, T Yokobori, T Shioya, N Teramae

CHEMISTRY LETTERS　(10)　1058-1059　2001/10

DOI： 10.1246/cl.2001.1058 　

ISSN： 0366-7022

eISSN： 1348-0715
A thiourea-based chromoionophore for selective binding and sensing of acetate Peer-reviewed

R Kato, S Nishizawa, T Hayashita, N Teramae

TETRAHEDRON LETTERS　42　(30)　5053-5056　2001/07

DOI： 10.1016/S0040-4039(01)00916-9 　

ISSN： 0040-4039
Selective potassium ion recognition by benzo-15-crown-5 fluoroionophore/y-cyclodextrin complex sensors in water Peer-reviewed

A Yamauchi, T Hayashita, A Kato, S Nishizawa, M Watanabe, N Teramae

ANALYTICAL CHEMISTRY　72　(23)　5841-5846　2000/12

DOI： 10.1021/ac000741i 　

ISSN： 0003-2700
A dibenzo-16-crown-5 fluoroionophore for selective emission ratio sensing of Na+ in basic aqueous dioxane solution Peer-reviewed

T Hayashita, S Taniguchi, Y Tanamura, T Uchida, S Nishizawa, N Teramae, YS Jin, JC Lee, RA Bartsch

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2　5　(5)　1003-1006　2000/05

DOI： 10.1039/a909638h 　

ISSN： 0300-9580
Positioning dependent anion recognition by thiourea-based chromoionophores via hydrogen bonding in aqueous vesicle solutions Peer-reviewed

T Hayashita, T Onodera, R Kato, S Nishizawa, N Teramae

CHEMICAL COMMUNICATIONS　(9)　755-756　2000

DOI： 10.1039/a909758i 　

ISSN： 1359-7345
Efficient ion-pair extraction of potassium chloride from aqueous phase into nitrobenzene containing dicyclohexyl-18-crown-6 as cation binder and diphenylthiourea as anion binder Peer-reviewed

MM Murad, T Hayashita, K Shigemori, S Nishizawa, N Teramae

ANALYTICAL SCIENCES　15　(12)　1185-1189　1999/12

DOI： 10.2116/analsci.15.1185 　

ISSN： 0910-6340
A thiourea-functionalized benzo-15-crown-5 for cooperative binding of sodium ions and anions Peer-reviewed

S Nishizawa, K Shigemori, N Teramae

CHEMISTRY LETTERS　(11)　1185-1186　1999/11

DOI： 10.1246/cl.1999.1185 　

ISSN： 0366-7022

eISSN： 1348-0715
Fluorescence sensing of anions via intramolecular excimer formation in a pyrophosphate-induced self-assembly of a pyrene-functionalized guanidinium receptor Peer-reviewed

S Nishizawa, Y Kato, N Teramae

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　121　(40)　9463-9464　1999/10

DOI： 10.1021/ja991497j 　

ISSN： 0002-7863
Benzo-15-crown-5 fluoroionophore/gamma-cyclodextrin complex with remarkably high potassium ion sensitivity and selectivity in water Peer-reviewed

A Yamauchi, T Hayashita, S Nishizawa, M Watanabe, N Teramae

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　121　(10)　2319-2320　1999/03

DOI： 10.1021/ja984164f 　

ISSN： 0002-7863
Complexation kinetics of 8-quinolinol derivatives with Ni(II) and Zn(II) at the 1,2-dichloroethane-water interface as studied by electrolyte ascending electrode polarography Peer-reviewed

T Shioya, S Nishizawa, N Teramae

LANGMUIR　15　(7)　2575-2579　1999/03

DOI： 10.1021/la9815547 　

ISSN： 0743-7463
Fluorescence ratio sensing of alkali metal ions based on control of the intramolecular exciplex formation Peer-reviewed

S Nishizawa, M Watanabe, T Uchida, N Teramae

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2　(2)　141-143　1999/02

ISSN： 0300-9580
Anion recognition at the liquid-liquid interface. Sulfate transfer across the 1,2-dichloroethane-water interface facilitated by hydrogen-bonding ionophores Peer-reviewed

T Shioya, S Nishizawa, N Teramae

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　120　(44)　11534-11535　1998/11

DOI： 10.1021/ja982854d 　

ISSN： 0002-7863
Anion sensing by a donor-spacer-acceptor system: an intramolecular exciplex emission enhanced by hydrogen bond-mediated complexation Peer-reviewed

S Nishizawa, H Kaneda, T Uchida, N Teramae

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2　(11)　2325-2327　1998/11

DOI： 10.1039/a805075i 　

ISSN： 0300-9580
Hydrogen-bonding ionophores for inorganic anions and nucleotides and their application in chemical sensors Peer-reviewed

P Buhlmann, S Amemiya, S Nishizawa, KP Xiao, Y Umezawa

JOURNAL OF INCLUSION PHENOMENA AND MOLECULAR RECOGNITION IN CHEMISTRY　32　(2-3)　151-163　1998/11

DOI： 10.1023/A:1008055225528 　

ISSN： 0923-0750
Complexation kinetics of 5-alkyloxymethyl-8-quinolinols at liquid-liquid interfaces as studied by dynamic interfacial tensiometry Peer-reviewed

T Shioya, S Nishizawa, N Teramae

LANGMUIR　14　(16)　4552-4558　1998/08

DOI： 10.1021/la9714059 　

ISSN： 0743-7463
Anion sensing by a thiourea based chromoionophore via hydrogen bonding Peer-reviewed

S Nishizawa, R Kato, T Hayashita, N Teramae

ANALYTICAL SCIENCES　14　(3)　595-597　1998/06

DOI： 10.2116/analsci.14.595 　

ISSN： 0910-6340
Application of a bis-thiourea ionophore for an anion selective electrode with a remarkable sulfate selectivity Peer-reviewed

S Nishizawa, P Buhlmann, KP Xiao, Y Umezawa

ANALYTICA CHIMICA ACTA　358　(1)　35-44　1998/01

DOI： 10.1016/S0003-2670(97)00569-2 　

ISSN： 0003-2670
A chloride ion-selective solvent polymeric membrane electrode based on a hydrogen bond forming ionophore Peer-reviewed

KP Xiao, P Buhlmann, S Nishizawa, S Amemiya, Y Umezawa

ANALYTICAL CHEMISTRY　69　(6)　1038-1044　1997/03

DOI： 10.1021/ac961035d 　

ISSN： 0003-2700
Strong hydrogen bond-mediated complexation of H2PO4- by neutral bis-thiourea hosts Peer-reviewed

P Buhlmann, S Nishizawa, KP Xiao, Y Umezawa

TETRAHEDRON　53　(5)　1647-1654　1997/02

DOI： 10.1016/S0040-4020(96)01094-0 　

ISSN： 0040-4020
Anion recognition by a pyrene derivative with a thiourea function Peer-reviewed

S Nishizawa, N Teramae

ANALYTICAL SCIENCES　13　485-488　1997

DOI： 10.2116/analsci.13.Supplement_485 　

ISSN： 0910-6340
STUDIES ON THE PHASE BOUNDARIES AND THE SIGNIFICANCE OF IONIC SITES OF LIQUID MEMBRANE ION-SELECTIVE ELECTRODES Peer-reviewed

P BUHLMANN, S YAJIMA, K TOHDA, K UMEZAWA, S NISHIZAWA, Y UMEZAWA

ELECTROANALYSIS　7　(9)　811-816　1995/09

DOI： 10.1002/elan.1140070905 　

ISSN： 1040-0397
ANION RECOGNITION BY UREA AND THIOUREA GROUPS - REMARKABLY SIMPLE NEUTRAL RECEPTORS FOR DIHYDROGENPHOSPHATE Peer-reviewed

S NISHIZAWA, P BUHLMANN, M IWAO, Y UMEZAWA

TETRAHEDRON LETTERS　36　(36)　6483-6486　1995/09

DOI： 10.1016/0040-4039(95)01296-T 　

ISSN： 0040-4039
CATION PERMSELECTIVITY IN THE PHASE-BOUNDARY OF IONOPHORE-INCORPORATED SOLVENT POLYMERIC MEMBRANES AS STUDIED BY FOURIER-TRANSFORM INFRARED ATTENUATED TOTAL-REFLECTION SPECTROMETRY Peer-reviewed

K UMEZAWA, XM LIN, S NISHIZAWA, M SUGAWARA, Y UMEZAWA

ANALYTICA CHIMICA ACTA　282　(2)　247-257　1993/10

DOI： 10.1016/0003-2670(93)80208-3 　

ISSN： 0003-2670

Show all ︎Show first 5

Misc. 14

Triplex-Forming Peptide Nucleic Acid Probe Having Thiazole Orange as a Base Surrogate for Fluorescence Sensing of Double-stranded RNA (vol 138, pg 9397, 2016)

Takaya Sato, Yusuke Sato, Seiichi Nishizawa

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　139　(9)　3567-3567　2017/03

DOI： 10.1021/jacs.7b01636 　

ISSN： 0002-7863
siRNA選択性蛍光プローブを用いた細胞内デリバリーイメージング解析

佐藤貴哉, 西澤精一

Dojin news　(157)　6-10　2016/06/28

More details Close

総説
シクロデキストリン修飾DNA複合体の協同的塩基認識デザイン

二村朱香, 伊本剛, 北村裕介, 佐藤雄介, 西澤精一, 井原敏博

シクロデキストリンシンポジウム講演要旨集　30th　184-185　2013/08/27
核酸分析を指向した協同的塩基認識プログラム

二村朱香, 伊本剛, 北村裕介, 西澤精一, 佐藤雄介, 寺前紀夫, 井原敏博

分析化学討論会講演要旨集　72nd　6　2012/05/05
核酸を足場とした特異的塩基認識機構のプログラミング

二村朱香, 伊本剛, 北村裕介, 城昭典, 西澤精一, 佐藤雄介, 寺前紀夫, 井原敏博

日本化学会講演予稿集　92nd　(3)　1047　2012/03/09

ISSN： 0285-7626
DNAの微小空間を利用する分析法の開発〜小分子結合リガンドの開拓と脱塩基部位含有DNAの活用〜

西澤精一

FBC Lett.　(36)　16-21　2011/07
日本分析化学会第59年会

寺前紀夫, 西澤精一, 藤原一彦

ぶんせき　(12月)　690-693　2010/12
規制反応場における化学センシング

寺前紀夫, 西澤精一

科学と工業　84　(9)　376-382　2010
心のギアチェンジ（リレーエッセイ）

西澤精一

ぶんせき　2008　(4)　189　2008/04/05
Thermodynamic characterization of the binding of naphthyridines to AP site-containing DNA duplexes

Yusuke Sato, Takehiro Seino, Seiichi Nishizawa, Norio Teramae

Nucleic Acids Symp. Ser.　50　219-220　2006
Use of Abasic Site-Containing DNA for Electrochemical SNPs detection

Kotaro Morita, Seiichi Nishizawa, Norio Teramae

Nucleic Acids Symp. Ser.　50　91-92　2006
DNA 脱塩基部位空間における核酸塩基認識と一塩基多型の蛍光検出

西澤精一, 寺前紀夫

ぶんせき　(11)　655-657　2004
蛍光性ヌクレオシドによる遺伝子変異検出（2003年の化学／注目の論文）

西澤精一

化学　58　(8)　51-52　2003/08/01
Publisher: （株）化学同人
水素結合性イオノフォアによる油水界面アニオン促進移動解析

西沢精一, 横堀倫之, 加藤亮

Proceedings of the Chemical Sensor Symposium　34　73-75　2002/04
Publisher: 電気化学会化学センサ研究会

Show all ︎Show first 5

Books and Other Publications 9

原書7版クリスチャン分析化学 I. 基礎編

G. D. Christian, P. K. Dasgupta, K. A. Schug, 著今任稔彦, 角田欣一監訳, 西澤精一ほか共訳

丸善出版株式会社　2016/12/30

ISBN: 9784621301098
理科年表平成29年（国立天文台編）

西澤精一

丸善出版株式会社　2016/11/30
化学フロンティア20 新しい地平をひらく分析手法の最前線

北森武彦編, 寺前紀夫, 西澤精一

2009/01/10

ISBN: 9784759807509
基礎教育シリーズ新版分析化学実験

本水昌二, 磯崎昭徳, 井原敏博, 櫻川昭雄, 善木道雄, 寺前紀夫, 西澤精一, 平山和雄, 三浦恭之, 森田耕太郎, 山口央

東京教学社　2008/04/01

ISBN: 9784808230456
文科系のための自然科学総合実験

西澤精一

東北大学出版会　2008/03/28

ISBN: 9784861630835
基礎分析化学実験（梅澤喜夫・本水昌二・渡会仁・寺前紀夫編）

西沢精一

2004
機器分析実験（梅澤喜夫・本水昌二・渡会仁・寺前紀夫編）

西沢精一

2002
分析化学実験（梅澤喜夫・本水昌二・渡会仁・寺前紀夫編）

西沢精一

1999
実用に役立つテキスト分析化学I・II(共訳)

NISHIZAWA Seiichi

1998

Show all Show first 5

Presentations 54

核酸結合プローブの精密設計とRNA検出・細胞内イメージング

日本化学会第97春季年会　2017/03/16

More details Close

慶應義塾大学日吉キャンパス
FLUORESCENCE SENSING OF DOUBLE-STRANDED RNA BY TRIPLEX-FORMING PEPTIDE NUCLEIC ACID PROBES HAVING THIAZOLE ORANGE AS A BASE SURROGATE International-presentation

Seiichi Nishizawa, Takaya Sato, Yusuke Sato

XVII International Symposium on Luminescence Spectroscopy (ISLS 2016)　2016/11/22

More details Close

Howard Civil Service International House, Taipei, Taiwan
Design and synthesis of RNA-binding fluorescent molecules for analytical applications International-presentation

Satellite International Mini Symposium on Middle Molecular Strategy in Sendai　2016/09/20

More details Close

IMRAM, Tohoku University
核酸結合リガンドによるRNA検出と細胞内イメージング

第71回応用化学セミナー（大坂府立大学大学院工学研究科）　2016/07/08
RNA検出・細胞内イメージングのための蛍光性核酸結合プローブの分子設計

第76回分析化学討論会　2016/05/28
核酸結合リガンドによるRNA検出と細胞内イメージング

日本分析化学会第64年会　2015/09/10

More details Close

九州大学伊都キャンパス
核酸結合リガンドによるDNA・RNAセンシングと細胞内イメージング

第86回化学センサ研究会　2015/08/27

More details Close

仙台市情報・産業プラザ
核酸結合リガンドによるDNA・RNA検出と細胞内イメージング

第13回ホスト・ゲスト化学シンポジウム　2015/06/06

More details Close

東北大学川内北キャンパス
水素結合を利用する分子認識と分析化学

OZAKI INVITATION: バイオ・光分析最前線　2015/04/28

More details Close

関西学院大学理工学部神戸三田キャンパス
Design and Synthesis of DNA- and RNA-binding Ligands for Gene Detection International-presentation

2015 Yonsei International Symposium on Nano-Bio Molecular Assembly　2015/01/09
RNA結合リガンドの合成と分析化学的応用

第13回化学系薬学若手研究者セミナー　2014/09/06

More details Close

東北薬科大学 70周年記念講堂
Design and Synthesis of DNA- and RNA-binding Ligands for Analytical Applications International-presentation

The Michinoku International Symposium on Analytical Science 2014　2014/08/25
核酸結合リガンドの合成と分析化学的応用

日本化学会東北支部秋田地区講演会　2014/02/21
Design and Synthesis of DNA- and RNA-binding Ligands for Analytical Applications International-presentation

The 3rd Dalian University of Technology-Tohoku University Joint Symposium in Chemistry　2013/12/18
Design and Synthesis of DNA- and RNA-binding Ligands for Analytical Applications International-presentation

International Symposium for the 70th Anniversary of the Tohoku Branch of the Chemical Society of Japan　2013/09/28
有機低分子化合物による核酸認識

第30回無機・分析化学コロキウム　2013/05/31
RNAを標的とする蛍光性小分子リガンド

日本分析化学会第61年会　2012/09/19

More details Close

特別シンポジウムバイオセンシングの最先端
DNA-Binding Small Ligand-Immobilized Surface Plasmon Resonance Biosensor for Detecting Single-Nucleotide Polymorphisms International-presentation

Sara Miura, Akinori Suzuki, Yukiko Fujimoto, Katsuya Ono, Qiang Gao, Norio Teramae

International Association of Colloid and Interface Scientists, Conference 2012　2012/05/13
核酸結合リガンドの開発と応用〜DNAからRNA検出へ〜

第13回機能構造と分析化学シンポジウム　2012/02/18
ハロゲン化に基づくDNA結合リガンドの機能制御

2011ハロゲン利用ミニシンポジウム（第4回臭素化学懇話会年会）　2011/11/25
DNA-Binding Small Ligand-Immobilized Surface Plasmon Resonance Biosensor Detecting Single-Nucleotide Polymorphisms International-presentation

Sara Miura, Akinori Suzuki, Katsuya Ono, Qiang Gao, Norio Teramae

The 38th International Symposium on Nucleic Acid Chemistry (ISNAC 2011)　2011/11/09
核酸／リガンド相互作用解析と分析化学的応用

平成23年度化学系学協会東北大会　2011/09/17
Abasic Site-Containing Duplex DNA for Analytical Applications International-presentation

Minjie Li, Yusuke Sato, Norio Teramae

IUPAC International Congress on Analytical Sciences 2011 (ICAS 2011)　2011/05/22
Abasic Site-Based DNA Aptamers for Analytical Applications International-presentation

Norio Teramae

2010環太平洋国際化学会議　2010/12/15
Small molecule-immobilized surface plasmon resonance biosensors for gene detection International-presentation

Norio Teramae

2010環太平洋国際化学会議　2010/12/15
核酸／リガンド相互作用解析と分析化学的応用

寺前紀夫

GEヘルスケア・ジャパンセミナー BIA Symposium in 仙台　2010/10/26
A Surface Plasmon Resonance Biosensor Based on a Small Ligand for Detecting Orphan Bases in Abasic Site-containing DNA Duplexes International-presentation

Norio Teramae

2nd Japanese-Russian Young Scientists Conference on Nano-Materials and Nano-Technology　2010/09/21
蛍光性核酸塩基修飾DNAアプタマーによるテオフィリン検出

李敏杰, 佐藤雄介, 寺前紀夫

日本分析化学会第59年会　2010/09/15
疎水場感受性蛍光色素を導入したナフチリジン誘導体の合成と(CNG)n トリヌクレオチドリピートDNA配列との相互作用解析

佐藤雄介, 本城温子, 石川大佑, 寺前紀夫

第24回生体機能関連化学シンポジウム　2009/09/13
蛍光性核酸結合リガンドを利用するアフィニティーラベル化と一塩基多型検出

佐藤雄介, 寺前紀夫

「テーラーメイド医療を目指したゲノム情報活用基盤技術」第5回公開シンポジウム　2009/08/03
特定領域研究「液液界面ナノ領域の化学」に関わって

分析化学若手交流シンポジウム　2009/05/17
蛍光性ヌクレオシド修飾DNAアプタマーによる高選択的テオフィリン検出

李敏杰, 佐藤雄介, 寺前紀夫

第70回分析化学討論会　2009/05/16
Characterization of the ligand-DNA interaction for analytical applications International-presentation

Teramae, Norio

Global COE and IREMC Symposium on Functional Materials and Analytical Approaches for Molecular Complex Chemistry　2008/11/05

More details Close

口頭（依頼）
脱塩基部位を基質結合サイトとするDNAアプタマー

李敏杰, 佐藤雄介, 中村洪大, 清野丈博, 寺前紀夫

第三回バイオ関連化学合同シンポジウム2008　2008/09/18
DNA二重鎖／有機リガンド相互作用解析と一塩基多型検出への応用

寺前紀夫

日本分析化学会第57年会　2008/09/10
DNA二重鎖／リガンド相互作用解析と分析化学的応用

「分離機能とセンシング機能の化学」セミナー2008　2008/03/22

More details Close

東北大学金属材料研究所
有機低分子リガンドによる核酸塩基認識と一塩基多型検出

第13回生物機能高分子専攻セミナー　2007/11/30

More details Close

北海道大学大学院工学研究科
有機低分子化合物による核酸塩基認識と一塩基多型検出

多元研講演会「核酸医薬への展開を目指した人工核酸の新しいサイエンス」　2007/05/18

More details Close

東北大学・多元物質科学研究所
DNA脱塩基空間における核酸塩基認識と遺伝子診断への応用

第18回分子構造若手夏の学校　2004/09/04
ここまで来たSNPs（一塩基多型）検出（化学センサー研究懇談会講演）

日本分析化学会第５3年会　2004/09/01
蛍光生小分子によるDNAセンシング（シンポジウム／バイオアナリシスの新展開）

日本分析化学会第５2年会　2003/09/23
水素結合性人工レセプターによる界面反応場イオン・分子認識

文部科学省科学研究費特定領域研究（B）「液液界面ナノ領域の化学」，第4回公開シンポジウム　2003/07/24
界面反応場を利用する分子・イオン認識

日本表面科学会東北支部講演会　2003/03/06
分子複合体形成に基づく分子・イオン認識

化学系7学協会連合東北地方大会　2002/10/05
分子複合体形成に基づく分析試薬の開発と液液界面イオン認識

日本分析化学会第５１年会　2002/09/19

More details Close

奨励賞受賞講演
界面反応場における分子・イオン認識

9) 文部科学省科学研究費特定領域研究（B）「液液界面ナノ領域の化学」，第2回公開シンポジウム　2002/07/29
水素結合性人工レセプターによるアニオン認識

第31回中部化学関係学協会支部連合秋季大会　2000/09/29
水素結合性レセプターによるアニオン認識

第16回分析化学緑陰セミナー　2000/07/15
水素結合性アニオン認識試薬の開発とその展開

日本化学会第78春季年会　2000/03/28

More details Close

進歩賞受賞講演
超分子構造に基づくアニオン認識

第3回機能構造と分析化学シンポジウム　1999/10/29
分子内エキサイプレックス発光を利用したイオンセンシング

第 4 回機能性ホスト-ゲスト化学研究会サマーセミナー　1998/07/23
ニュートラルレセプターによる水素結合を介したアニオン認識

第 15 回無機分析コロキウム　1998/06/18
水素結合を利用した人工アニオンレセプター

第20回分析化学若手交流会　1997/07/04
人工レセプターによる水素結合を利用したアニオン認識

「機能構造と分析化学」セミナー　1997/05/28

Show all Show first 5

Industrial Property Rights 2

遺伝子変異検出方法及び遺伝子変異検出用キット

寺前紀夫, 西澤精一, 吉本敬太郎, 清野丈博, 佐藤冬樹

特許第4638419号

Property Type: Patent
一塩基置換検出方法及び一塩基置換検出用キット

西澤精一, 寺前紀夫, 吉本敬太郎

特許第4320188号

Property Type: Patent

Research Projects 35

分子プローブによる環境中ウイルス粒子の検出・可視化技術の創製

西澤精一, 永富良一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的研究(開拓)

Institution: 東北大学

2021/07/09 - 2024/03/31

More details Close

本研究では、ウイルス粒子表面のエンベロープ構造及び内包されているゲノムRNAを標的とする蛍光性分子プローブ（検出試薬）をそれぞれ設計・合成し、両者の併用に基づく全く新しいウイルス粒子検出・可視化（イメージング）技術を開発する。具体的には、（i）ウイルス粒子表面の高曲率脂質二重膜（エンベロープ：直径約100 nm）に高選択的に結合・蛍光応答を示す両親媒性α-helixペプチドプローブ、及び（ii）ウイルス粒子のエンベロープ構造を迅速に透過・内部に拡散し、ゲノムRNAに特異的に結合・蛍光応答を示すRNA染色蛍光色素を開発する。２種類の蛍光性分子プローブを併用することで、環境中ウイルス粒子（エンベローブ構造＆内包RNA）の存在をダブル検出（２重染色）できる簡易技術を開発、医療現場やウイルス研究で用いられる様々な材料に付着したウイルス検出へと適用することで方法論としての基礎と有用性を実証する。令和3年度の研究実績の概要は下記のようになる。 (a) 新規生細胞RNA染色蛍光色素として、世界トップレベルの高輝度モノメチンシアニン色素の開発を達成した（特願2021-134803：特許第7029841号）。新規に開発したRNA染色蛍光色素は、off-on型の優れたlight-up応答機能と膜透過性を併せ持ち、生細胞中のRNA（核小体）を明瞭に可視化（イメージング）することができる。 (b) 本研究課題に関連して、RNAが脂質膜に内包された製剤（ワクチン）の品質管理技術を提案した（PCT/JP2021/030716）。
Development of triplex-forming peptide nucleic acid probes for advanced RNA analysis

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2020/04/01 - 2023/03/31
Design and synthesis of red fluorescent RNA-binding indicators and their application to high-throughput screening for drug discovery

NISHIZAWA Seiichi, SATO Yusuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Tohoku University

2017/06/30 - 2019/03/31

More details Close

We have successfully discovered that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, functions as a deep-red fluorescenct indicator for the internal loop structure of the bacterial (Escherichia coli) ribosomal decoding region of the aminoacyl-tRNA site (A-site), which enables the assessment of A-site binding capability of various test compounds including blue and even-green-emitting compounds (Chem. Commun. 2019). TO-PRO-3 can also work as a useful indicator for the assessment of the binding capabilities of various test compounds for Influenza A virus RNA promoter region with a view toward the development of anti-influenza drug candidates (ChemBioChem. 2019). In addition, we have developed a new class of small heterocyclic ligands based on the 2-amino-5,6,7-trimethyl-1,8-naphthyridine structure for the bacterial A-site (Chem. Eur. J. 2018). Significantly, this ligand shows the tightest binding reported to date among non-aminoglycoside ligands.
Design, synthesis and analytical application of peptide-based RNA-binding ligands with high affinity and selectivity

NISHIZAWA Seiichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2016/04/01 - 2019/03/31

More details Close

We recently proposed a new strategy for analyzing the siRNA delivery process based on affinity labeling with a peptide nucleic acid (PNA)-based fluorescent probe capable of selectively binding to the overhanging structures of siRNAs (Chem. Commun. 2015). Here, we have successfully prepared a new probe with improved binding affinity and imaging ability by conjugation with a cationic oligopeptide (ChemBioChem 2019). In addition, we have developed new fluorogenic sensing probes for double-stranded RNA (dsRNA) by integrating thiazole orange (TO) as a base surrogate into triplex-forming PNA (J. Am. Chem. Soc. 2016; Chem. Eur. J. 2017; Org . Biomol. Chem. 2017; Org . Biomol. Chem. 2018; RSC Advances 2018). Furthermore, we have developed useful fluorescent indicators for the assessment of the binding capabilities of various test compounds for the internal loop structure of the bacterial ribosomal decoding region of the aminoacyl-tRNA site (A-site) (Chem. Eur. J. 2018; Chem. Commun. 2019).
Development of a Bio-Raman Microscope to Enable Long Term Monitoring of Single Cells

Shin-ichi Morita

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2015/04/01 - 2018/03/31

More details Close

Recently it became possible to measure Raman spectra of a single live cell using a standard Raman microscope. We developed a bio-Raman microscope in order to monitor the dynamics of cellular differentiation, proliferation, and apoptosis. To do so, living cells were incubated on the microscope stage. Using the bio-Raman microscope, we defined cellular states in a non-destructive and non-labeling manner. The obtained information of cellular conditions is very important to control cellular destinies aiming tissue engineering. Advanced mathematical methods, developed in this project, were found effective to spectral analysis in a general manner.
Development of boronate affinity electrophoresis for quantitative separation and analysis of Piwi-interacting RNA

Nishizawa Seiichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2015/04/01 - 2017/03/31

More details Close

Piwi-interacting RNA (piRNA), single stranded RNA of 26-31 nucleotide length, is a new class of small noncoding RNA which is mainly expressed in the germline. Their 3’-terminal nucleotides feature 2’-O-ribose methylation by 3’methyltransferase HEN1, which can enhance the piRNA stability. It has been known that piRNA plays a key role in the protection of germ cell genomes from the mobile genetic elements, thereby preventing transposon-induced defects in gametogenesis and fertility. In this work, we have successfully improved boronate affinity electrophoresis for piRNA separation in the biological RNA samples, which is based on specific capture of boronic acid in the gel for cis-diol group in 3’terminal ribose of RNA species other than piRNAs having 2’-O-methylated ribose. Our improved method allows piRNA separation from mouse testes total RNA in a single step manner, revealing the great potential for quantitative separation and analysis of piRNA.
Development of fluorescent small probes for RNA imaging in cells

NISHIZAWA Seiichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2013/04/01 - 2015/03/31

More details Close

In this work, synthetic fluorescent probes are successfully developed for intracellular delivery analysis of small interfering RNAs (siRNAs). The probes are based on peptide nucleic acids that are conjugated with thiazole orange (TO) and pyrene to their C- and N-termini, respectively. The probe is able to selectively bind to 3’-overhanging two nucleotides uniquely found in siRNAs, and the binding is accompanied by a significant light-up response due to intercalation of the TO moiety into the double-stranded regions near overhangs. In contrast to typical methods using fluorophore-labeled siRNA, the present probe does not compromise the siRNA silencing activity, and is applicable to the analysis of both cellular uptake and the intracellular disassembly of the polyplexes. The use of the probe thus allows a more detailed and quantitative analysis of the siRNA delivery process, which would be very useful for siRNA-related study.
Development of fluorescent ligands for the analysis of non-coding RNA

NISHIZAWA Seiichi, SATO Yusuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2012/04/01 - 2015/03/31

More details Close

In this work, new analytical methods using synthetic fluorescent ligands are successfully developed for the study of non-coding RNA, including trans-activation-responsive region (TAR) RNA of human immunodeficiency virus type 1 (HIV-1), microRNA, and small interfering RNA (siRNA). First, we report on promising binding and signaling functions of thiazole orange (TO) to recognize TAR RNA. We found that TO does bind to selectivity TAR RNA with a nanomolar affinity, and TO was utilized as a fluorescent indicator for the high-throughput screening assay to identify new class of scaffolds of HIV inhibitors. As for the detection of microRNA in vitro, fluorescent ligands are designed and synthesized based on abasic site-binding ligands that are conjugated with cyanine dyes. Furthermore, fluorescent probes are successfully developed based on peptide nucleic acids for intracellular delivery analysis of siRNA.
Development of Micro Arrays for Analyzing Small RNAs

TERAMAE Norio, YAMAGUCHI Akira, NISHIZAWA Seiichi, SATO Yusuke, XU Zhiai

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (S)

Institution: Tohoku University

2010/04/01 - 2015/03/31

More details Close

This work aimed to develop new strategy of small RNA analysis based on the RNA-binding fluorescent ligands in confined nanopores. Toward this end, we focused on the design of fluorescent abasic site (AP site)-bidning ligands (APLs) in RNA or DNA/RNA duplexes. We successfully found useful G, C, A, or U-discriminating candidates targeting the duplexes. Moreover, new class of light-up fluorescent ligands was developed based on the conjugation of APLs with cyanine dyes. On the other hand, we revealed several unique features of confined spaces inside the silica and alumina nanopores. Especially, exothermic reactions such as cyclodextrin inclusion and DNA hybridization were found to be accelerated inside the nanopores under supercooled condition. Finally, we found possible applications to detect dozens of attomole nucleic acids and analyze their sequences with single nucleotide resolution by the binding of APL-cyanine dye conjugates inside the confined nanopores under supercooled conditions.
RNA非翻訳領域を標的とする核酸検出リガンドの創製と機能性RNA研究への応用

寺前紀夫, 西澤精一, 徐志愛, 佐藤雄介

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(A)

Institution: 東北大学

2010 - 2010

More details Close

タンパク質に翻訳されずに機能するRNAは,ノンコーディングRNA (ncRNA)と呼ばれる。ここ10年程の間に,ncRNAが関与する生物現象の発見が相次ぎ,生物学の知見が新しいものに塗替えられつつある。なかでもリボスイッチは,低分子化合物との特異的結合によって遺伝子発現調節を行うユニークなncRNAで,抗生物質の新たな標的として,あるいは遺伝子発現を制御しうる技術開発に繋がる研究対象として,特に注目されているncRNAファミリーの1つである。本研究では,新たな研究領域への新たな分析ツールの提供を目指し,『ノンコーディングRNA,特にリボスイッチを標的とする蛍光性の小分子(リガンド)を開発し,これらの化合物に基づくRNA解析法並びに薬剤ハイスループットスクリーニング法を開発する』ことを目的とする。また,リボスイッチの他,『ヒト免疫不全ウイルス(HIV)のTrans-activation responsive region (TAR RNA)や16SリボソームRNAAサイト』といった遺伝子発現調節に関わるRNAの非翻訳領域を標的とする蛍光性リガンド開発も併せて試みる。本年度(平成22年4月～5月)は,ピラジン誘導体(蛍光極大波長:～400nm)が抗生物質とほぼ同等の結合親和力でTAR RNAと結合しうることを見出すとともに(K_d=770nM),蛍光性ナフチリジン誘導体(蛍光極大波長:～400nm)がアミノグリコシド類に匹敵する結合力でAサイトに結合することを見出した(K_d=530nM)。現在,より詳細な相互作用解析を進めており,それらの知見に基づいて結合機能及び蛍光応答特性の改良をさらに行うことで,ncRNAの新しい分析ツールになることが期待できる。
RNA/DNAハイブリッド特異的結合リガンドの創製と小分子RNA解析

西澤精一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的萌芽研究

Institution: 東北大学

2009 - 2010

More details Close

本研究は、『マイクロRNA(miRNA)を標的とする核酸結合リガンドを創製、これをベースにPCR増幅を不要とするmiRNA解析法を開発すること』を目的とする。具体的には、miRNAと相補的な塩基配列を有する脱塩基部位含有プローブRNA(あるいはDNA)をハイブリダイゼーションさせることで、RNA/RNA二重鎖(RNA/DNAハイブリッド)を形成、形成したRNA/RNA二重鎖(RNA/DNAハイブリッド)を蛍光性リガンドで検出するもので、核酸プローブ中に脱塩基部位を設けることで、標的RNA/RNA二重鎖(RNA/DNAハイブリッド)の特異的かつ高感度検出が可能になると期待できる。昨年度までに、蛍光性ジアミノピラジン誘導体(アミロライド)をmiRNA検出に適用しうることを見出していたが(励起380nm、検出414nm)、本年度は、アミロライドをベースとして蛍光応答特性の改良を進めた。その結果、疎水場感受性色素(DBD)をジアミノピラジン骨格に連結することで、長波長検出(～550nm)に対応した蛍光性リガンドを開発することに成功した。1et-7aを標的とした場合、0.22pmol(1.2ng)のmiRNA検出が可能であり、既存のノーザンブロッティング法とほぼ同程度の検出感度(～ng)を有している。今後、さらにリガンドの改良を進めることで、PCR増幅を不要とするmiRNA解析法になりうると期待できる。
Development of DNA-binding ligands detecting trinucleotide repeats

NISHIZAWA Seiichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2008 - 2010

More details Close

Recent molecular genetic studies have shown that expansion of DNA trinucleotide repeats cause human neurological diseases, which are called trinucleotide repeat disorder. Unfortunately, there are no effective therapeutic approaches for the treatment of these diseases. In this work, we have successfully developed fluorescent ligands for C-C mismatch detection in CCG trinucleotide repeats that are associated with fragile site XE-linked mental retardation. It is expected that the discovery of such small molecules that bind trinucleotide repeats would provide a method for the smart diagnosis of the repeated length and a rational basis for the development of effective therapeutic agents.
新しい核酸結合試薬の創製とDNA損傷のバイオイメージング

西澤精一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 萌芽研究

Institution: 東北大学

2007 - 2008

More details Close

本研究は, 蛍光性DNA結合試薬(リガンド)を利用するDNA損傷検出法の開発を目的とする。具体的には, DNA損傷修復過程の中間体として, あるいは塩基損傷に誘起される二重らせん構造の歪みにより生成する(疑似)脱塩基部位を, DNA損傷のマーカーとして「蛍光性リガンドにより検出すること」を試みる。平成20年度は, (1) チミン欠損を検出しうる蛍光性リガンドの開発を達成するとともに, (2) DNA損傷検出表面プラズモン共鳴(Surface Plasmon Resonance, SPR)センサーを開発した。 (1) チミン欠損を標的とする場合, 対面塩基となるアデニンに対する結合リガンドを開発する必要があるが, アデニンとはワトソン・クリック型の塩基対として二点水素結合形成のみが可能であるため、アデニンに対する結合選択性と高親和力を発現させることが困難な状況にあった。ここでは, 種々の化合物をスクリーニングした結果, アデニンへの二点水素結合形成部位を有し, かつリガンドサイズを制御することで、アデニン(チミン欠損)検出リガンド(アロキサジン及びジメチルルマジン)を開発することに成功した。 (2) 一方, 1, 8-ナフチリジン誘導体やプテリジン誘導体, 3, 5-ジアミノピラジン誘導体を新規合成し, これらを感応素子として利用することで, 高感度・高選択的なDNA損傷検出SPRセンサーを開発した。ここでは, 核酸塩基認識プローブ(DNA結合リガンド)を金基板チップ上に配列・固定化したセンサー(フローセル型)を開発したもので, グアニン欠損及びシトシン欠損, アデニン欠損を検出することが可能である。いずれのセンサーも, 標的欠損含有DNAに対してほぼ特異的なSPR応答を示し, また, 蛍光法と比較してより低濃度のDNA解析に適用可能である。
Molecule recognition interface with specifity and its application for chemical sensing

TERAMAE Norio, NISHIZAWA Seiichi, YAMAGUCHI Akira

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2005 - 2008
Development of Abasic Site-Containing DNA Duplex Aptamers for Biologically Important Substrates

NISHIZAWA Seiichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2006 - 2007

More details Close

Aptamers, short nucleic acid-binding species, have emerged as promising candidates for molecular recognition events on account of their significant ability to bind large number of ligands, including amino acids, ribose-containing cofactors and drugs. High affinity and specificity of these aptamers toward target ligands are generally achieved by a combination of molecular shape complementarity, hydrogen-bonding and stacking interactions. Such binding events typically involve the ligand-induced structural changes of the aptamers, resulting in the formation of unique secondary structures responsible to the ligand binding, for which the internal - or stem-loop structures are representative of active sites for binding events. However, for the further design of both RNA and DNA aptamers for small ligands, little attention has been paid to other types of non-base-pairing moieties despite the potential as binding pockets. Here we report on a new class of DNA duplex aptamers for selective and strong binding to biologically important ligands, where an abasic (AP) site in the duplex is utilized as an active cavity for binding to biologically important ligands, where an abasic (AP) site in the duplex is utilized as an active cavity for binding events. For the AP site-based DNA aptamers, it is strongly expected that, by stacking with two nucleotides flanking the AP site, a target ligand is intercalated into the AP site, and this is accompanied by the formation of hydrogen bonds with a receptor nucleotide opposite the AP site. Indeed, we have successfully developed this type of aptamers with high selectivity for riboflavin (dissociation constant K_d=1.9μM), theophylline (K_d=31μM), caffeine (K_d=20μM), and adenosine (detection range: 10~150μM) in this research project. As compared to typical RNA aptamers, our AP site-based DNA aptamers have some advantages, such as their easy and low-cost synthesis, and higher chemical stability. These features would allow various kinds of analytical applications.
グアノシン高次会合構造の形成制御とバイオ分析への応用

寺前紀夫, 西澤精一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 萌芽研究

Institution: 東北大学

2005 - 2006

More details Close

分子認識液晶を開発するために、液晶分子の配向制御部位および、分子認識部位を併せ持つグアノシン誘導体を長鎖アルキル基を有するアゾベンゼンを置換基として新規合成し(6AG)、その機能を評価した。その結果6AGのリボン型自己集合体が7CB液晶分子の配向を制御しうる水素結合型液晶構造の構築を達成した。さらにこれを臨床的に必須な測定基質であるクレアチニン検出に適用しうることを見出した。ネマティック液晶相を示す7CB単体に対して、6AGモル比の異なるブレンド試料を4種(約5,10,15,30mol%)作製し、偏光顕微鏡観察を行った。その結果、低6AG比の試料ではfinger print組織が、高6AG比の試料ではlamella組織が観察されることから、それぞれキラルネマティック(N^*)液晶相およびスメクティック相(Sm)液晶相が発現していることが分かった。低6AG比でのN^*相発現には、6AGの糖部位由来の光学活性が関与しているものと考えられる.一方、Sm相の発現は6AGリボン型自己集合体形成に起因するものと考えられる。顕微IRスペクトル測定による検討では、カルボニル吸収帯が低波数シフトすることから、グアニン間の水素結合形成が示唆された。また、X線構造回折測定から層構造の存在が支持され、さらに得られた層の周期構造(55.9Å,38.9Å)は,分子モデリングによる6AGの自己集合体の分子サイズとよく一致した。以上により、6AGの相溶比を変化させることにより、7CB単体では発現しえない液晶構造を構築した。約30mol% 6AG/7CBのSm液晶について、そのクレアチニン検出機能を検討した結果、クレアチンやグルコース、尿素などの妨害基質には画像変化を全く示さないのに対し、クレアチニンに対して明瞭な画像変化を示すことが分かった。
蛍光性有機小分子プローブによるDNA損傷検出

西沢精一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 若手研究(B)

Institution: 東北大学

2004 - 2005

More details Close

本研究は,有機小分子プローブを利用するDNA損傷蛍光検出法の開発を目的とする。具体的には,DNA損傷の修復過程において"脱塩基部位"が出現することに着目し,これをDNA損傷の"マーカー"として蛍光性小分子プローブにより検出するもので,既に,小分子プローブとしてAMND (2-amino-7-methyl-1,8-naphthyridine)を用いることにより,脱塩基部位生成の有無を簡便に蛍光検出(励起波長:350nm,検出波長:410nm)できることを見出している。しかし,本系をより優れたシステムとするためには,(1)蛍光応答特性の改善(励起波長の長波長化や発蛍光・レシオタイプにすること),(2)結合定数をより強化すること,が必要である。上述の課題を克服するために,本年度は,1,8-ナフチリジン骨格に蛍光団としてニトロベンゾキサジアゾール(NBD,検出波長:544nm)基を導入したプローブ(Naph-NBD)を合成し,その機能を評価した。その結果,分析化学的に有用なレシオメトリック解析(409nm/544nm蛍光強度比解析)が可能な検出プローブとして機能することが分かった。また,より長波長励起が可能な検出プローブとして,新たにリボフラビン(励起波長:448nm,検出波長:530nm)が機能することを見出した。さらに,DNAのリン酸部位への結合サイトとしてグアニジニウム基を有するアミロライドが,脱塩基部位含有DNAに対して非常に強力な親和性を発現すること(解離定数 150nM)を見出した。以上のように,優れた蛍光特性及び結合親和力を有するDNA損傷検出用の小分子プローブ群の開発を達成した。
Exploitation of Reaction Fields for Analytical Chemistry to Realize Specific Function for Molecule Recognition and Development of Chemical Sensing Systems

TERAMAE Norio, NISHIZAWA Seiichi, YAMAGUCHI Akira, ZHOU Hao-shen

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2002 - 2004

More details Close

In combination with abasic site (AP site)-containing oligodeoxynucleotides (ODNs), a potential use of a hydrogen bond forming ligand, 2-amino-7-methyl-1,8-naphthyridine (AMND), has been demonstrated for the fluorescence detection of the cytosine (C)/guanine (G) mutation sequence of the cancer repression gene p53. An AP site-containing ODN is hybridized with a target ODN so as to place the AP site toward a target nucleobase, by which hydrophobic microenvironments are provided for ligands to recognize target nucleobases through hydrogen-bonding. To increase the binding constant, a methyl group was attached to AMND and its detection ability for SNPs was examined. As a result, high affinity was achieved as expected. The effect of the structure of a hydrophobic site in an oligo-DNA duplex on the affinity was also examined. Potential use of a surfactant-like receptor is demonstrated at the 1,2-dichloroethane-water interface for strong and selective binding of H_2PO_4^-over Br^-and Cl^-. The analysis by interfacial tensiometry reveals that the interfacial adsorption of a thiourea-isothiouronium conjugate, BT-C1, is significantly stabilized by the binding of H_2PO_4^-with the adsorption constant of 1.7x10^5 M^<-1>, while the interfacial adsorptivity of this receptor is relatively moderate for Br^-and Cl^-. Such complexation-induced interfacial adsorption behaviors of BT-C1 are discussed as a basis for the development of receptor-based chemical sensors for phosphate anions A mesoporous membrane composed of nanochannels with a uniform diameter has a potential use for precise size-exclusive separation of molecules. Here, a novel method is reported to form a hybrid membrane composed of silica-surfactant nanocomposite and a porous alumina membrane, by which size-selective transport of molecules across the membrane becomes possible. The nanocomposite formed inside each columnar with nanocomposites Alumina pore was an assembly of surfactant-templated silica-nanochannels with a channel diameter of 3.4 nm ; the channel direction being predominantly oriented along the wall of the columnar alumina pore. Molecules could be transported across the membrane including the silica-surfactant nanocomposite with a capability of nanometer-order size-exclusive separation. Our proposed membrane system has a potential use not only for separation science, but also catalysis and chip technologies.
液液界面の特異性を用いる分子認識とSHG分光法による認識機能解析

寺前紀夫, 内田達也, 山口央, 西沢精一, 石坂昌司, 石岡寿雄

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 特定領域研究

Institution: 東北大学

2001 - 2003

More details Close

本年度の研究の遂行によって液液界面,液膜界面における相関移動あるいは膜透過といった分子の輸送現象,あるいは溶媒和現象を観測することで,液液界面を利用した分子認識機能について検討を行い,以下のような知見を得た。チオ尿素基,チオウロニウム基をアニオン認識部位に持つ一連のレセプターを合成し,液/液界面における特異的分子認識能について検討した。ニトロベンゼン/水系において,ビスチオウレア型のレセプターがリン酸イオンと特異的な1:2錯体を形成し,界面を横切る相間移動を起こすことをポーラログラフィー法により明らかとした。昨年度検討したレセプターよりもリン酸イオンに対する錯形成能は若干小さいものの,高効率なリン酸イオンの相関移動を達成する上で水素結合に起因する安定なレセプター:リン酸イオンの1:2錯体の形成が重要であることが示唆された。また,種々の有機溶媒を用いて形成した液液界面系にアンスロイルオキシ基を吸着させ,時間分解全反射蛍光測定を行うことで,液液界面に吸着した分子周囲の誘電率が優先的溶媒和現象に依存することを見いだした。液膜界面における分子の吸着,膜透過過程についてSHG分光法および蛍光分光法を用いて検討した。脂質二分子膜で構成されるベシクルを水溶液中に分散させ,一連のヘミシアニン分子の膜吸着,膜透過過程を観測したところ,カチオン性のヘミシアニン分子に比べて双性イオン型のヘミシアニン分子の膜透過が非常に遅いことを見いだし,また,膜透過速度がヘミシアニン分子のアニリン部位に結合したアルキル鎖の長さが短いほど膜透過が遅くなることを明らかとした。
フーグスティン塩基対形成型DNA蛍光プローブに基づく点突然変異遺伝子分析法の開発

西沢精一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 若手研究(B)

Institution: 東北大学

2001 - 2002

More details Close

本研究は,SNPs(Single Nucleotide Polymorphisms)蛍光検出法の開発を目的とする。本研究では,特に,DNAがフーグスティン型の水素結合形成により三重らせん構造をとることに着目し,フーグスティン型塩基対形成部位を有する蛍光性水素結合型レセプター分子の合成を行うとともに、DNA脱塩基部位を利用する核酸中一塩基認識法を開発した。つまり,脱塩基型合成DNAをハイブリダゼーションさせることで標的塩基周辺に意図的に疎水場空間を構築させた後,同空間中において水素結合型レセプターとの錯形成を行うというものである。水素結合型レセプターとして二つの芳香環を持つナフチリジン,キノリン誘導体を合成し,各レセプター分子添加前後におけるDNA二重鎖の融解温度(Tm)を測定した。その結果,Tmの著しい増加が観測されることから,レセプター分子が脱塩基部位中において核酸塩基を認識し得ることが示唆された。さらに興味深いことは,Gと相補的な3点の水素結合部位を持つナフチリジン誘導体が,CやTのピリミジン塩基に対して高い選択性を示す点である。このようなバルク溶液系とは異なる反応性は,本系における核酸認識が水素結合形成と脱塩基部位の空間的な規制による協同的な効果により達成されているためで,特に同空間の上下に位置する核酸塩基とのスタッキング相互作用が大きな役割を果たしていると考察している。CDスペクトル測定およびMacroModelを用いた分子力場計算の結果も上記考察を支持するものであった。また、ナフチリジン誘導体を用いることで、C特異的な蛍光検出を達成した。以上のように本研究では,DNA脱塩基部位(AP site)を利用することにより、水素結合型レセプターによる点突然変異蛍光検出法の開発に成功した。本研究で提案する脱塩基型合成DNAを用いる核酸塩基認識法は,脱塩基部位やレセプター分子を適切に設計・合成することで種々の塩基配列に適用可能であり,簡易且つ迅速な遺伝子診断法としての展開が期待できる。
Chemical Sensors based on Higher-Ordered Structures of Abiotic Nucleobases

TERAMAE Norio, UCHIDA Tatsuya, YAMAGUCHI Akira, NISHIZAWA Seiichi, KAGAWA Yasuhiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2001 - 2002

More details Close

1. Template-assisted preferential formation of syn photodimer in a pyrophosphate-induced self-assembly of a thymine-functionalized isothiouronium receptor: The effect of anion-templation is investigated for the photodimerization of a thymine-functionalized isothiouronium receptor. The receptor forms a photodimer at the thymine moiety is methanol upon the UV-irradiation, while the isothiouronium moiety works as on oxoanion binding site via o two-point hydrogen-bonding motif. As compared to the case of free receptor, the presence of pyrophosphate (PPi) resulted is the preferential formation of the cis-syn type photodimer, which would be desirable to recognize the templated PPi. Fluorescence Detection of Creatinine by a Hydrogen-Bonded Guanosine Octamer: We report the design and characterization of guanosine-based octamers held together by twenty-four hydrogen bonds and stacking interaction, capable of fluorescent detection of a clinically important substrate. The octamers consist of two cyclic guanosine-quartets, an formed by Hoogsteen base-pairing, and also by hydrogen bonds two quartets are coupled together so as to stack with a counterclockwise rotation. When creatinine binds to guanosine of the octamer by three hydrogen bonds similar to Watson-Crick base-pairing, the octamer labelled with pyrene dissociates to give a monomeric form accompanied by the change in visible fluorescence from green to purple. Use of A basic Site Containing DNA Strands for Nucleobase Recognition in Water: Nucleobase recognition in water is successfully achieved by the use of an abasic site (AP site) as the molecular recognition field. We intentionally constructed the AP site is DNA duplex so as to orient the AP site toward a target nucleobase, and examine the complexation of 2-amino-7-methylnaphthyridine (AMND) with nucleobases at the AP site. AMND is found to selectively bind to cytosine base (K_<11>>10^6M^1) accompanied by remarkable quenching of its fluorescence, and is available for selective and visible detection of a single-base alternation related to the cytosine base.
一次元ナノ細孔体を用いた生体関連イオン・分子の一斉分析システムの開発

寺前紀夫, 西沢精一, 内田達也

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 萌芽的研究

Institution: 東北大学

2000 - 2001

More details Close

シリカで形成された一次元ナノ細孔体MCM-41を有機溶媒に代わる分子認識場として利用し,生態関連物質に対する新規検出法を確立した。粒子状の細孔体を分子認識場とするために次の二つの方法を検討した。第一の方法では、チオ尿素基を有するシランカップリング剤を新規に合成し、細孔内を化学修飾した。標的分子としてATP類を用い、それらがリン酸基数を反映してチオ尿素基によって選択的に検出されることを明らかにした。第二の方法ではMCM-41細孔内に鋳型として形成された界面活性剤棒状ミセルに対するアルカリ金属イオンの抽出を試みた。クラウン骨格を有する蛍光性の分子認識試薬を用いて抽出を試みたところ、水中のアルカリ金属イオンが選択的に細孔内のミセルに取り込まれることを見いだした。以上の結果はナノ細孔を利用した物質検出法が水溶液中の生態関連物質の分析に有効であることを示している。さらに、分子認識機能を高度に組み合わせた一斉分析システムを構築するために、ナノ細孔構造を有する薄膜を試作し、薄膜における分子認識実験を行った。スピンコーティング法によって作製した薄膜のX線回折の結果から、粒子と同様に細孔がハニカム上に配列した構造をとることが明らかとなった。薄膜作製時に形成された細孔内の棒状ミセルを分子認識場として用いてカリウムの選択的検出を試みたところ、粒子を用いた場よりも高感度な検出が可能となった。今後、選性の異なる様々な薄膜を同基板上に配列することにより、生体関連物質の一斉検出を目指す。
Design of Artificial Cell Membrane Interface and Development of Evaluation System for Biorecognition Function

HAYASHITA Takashi, NISHIZAWA Seiichi, UCHIDA Tatsuya

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2000 - 2001

More details Close

To develop an anion sensing system in water based on a chromoionophore/artificial cell membrane conjugate, novel thiourea-based chromoionophores having various lengths of alkyl chains (Cn-TU) were designed and their anion recognition function in aqueous vesicle solutions were evaluated. This study revealed that the positioning of chromophore binding sites inside the didodecyldimethylammonium bromide (DDAB) vesicle was successfully controlled by the alkyl chain length of Cn-TU. The cationic vesicle interface was found to exhibit a filter function for the less hydrophobic anions, and highly selective anion recognition via hydrogen bonding was achieved by C1-TU located deep inside the DDAB vesicle. Function of crown ether chromoionophores for alkali metal ion recognition in micellar system were also examined. The surface charge of the micelle was found to strongly influence the ion selectivity and sensitivity, and selective Na^+ recognition was only performed by anionic micellar system. To evaluate ion recognition at these membrane interfaces, a resonant second harmonic generation (SHG) analysis system was developed by using 15-crown-5 azophenol as a model probe. Selective sorption of alkali metal complexes with the azoprobe at heptane/water interface was confirmed by SHG spectra, and their conformation changes upon metal recognition at the interface were successfully evaluated by SHG analysis.
Design and Function of Molecular Complex Sensors Based on Supramolecular Structure

HAYASHITA Takashi, NISHIZAWA Seiichi, UCHIDA Tatsuya

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2000 - 2001

More details Close

This study reports the supramolecular function of fluorescent probe/cyclodextrin (CyD) complexes for ion and molecule recognition in water. Benzo-15-crown-5 fluoroionophores, Cn-15C5 (n=1,3,5), with different alkyl spacer lengths were first examined to develop supramolecular Cn-15C5/γ-CyD complex sensors for alkali metal-ion recognition in water. In organic solutions, C3-15C5 shows moderate Na^+ selectivity based on 1:1 complex formation. However, the C3-15C5/γ-CyD complex is found to selectively respond to K^+ ion in water and to exhibit pyrene dimer emission. An equilibrium analysis of the γ-CyD inclusion complexes in water reveals that the major component for the dimer emission is a 2:1:1 complex of C3-15C5 with K^+ and γ-CyD. Although the K^+ sensitivity of the C5-15C5/γ-CyD complex is comparable to that of the C3-15C5/γ-CyD complex, it also responds to Na^+. The fluoroionophore C1-15C5, which has the shortest methylene spacer, exhibits no response to alkali metal cations in the presence of γ-CyD. Thus, the response function is strongly affected by the alkyl spacer length of Cn-15C5, and the highest K^+ selectivity in water is obtained for the C3-15C5/γ-CyD complex. The boronic acid fluoroionophore C4-PB/β-CyD complex binds sugars and produces increased fluorescence emission in water. A pH-fluorescence profile for the C4-PB/β-CyD complex reveals that the fluorescence intensity increases upon the formation of the boronate conjugate base. Upon the addition of fructose, the apparent pK_a decreases to a lower pH, resulting in increased fluorescence at neutral pH. The fluorescence emission response of the C4-PB/β-CyD complex upon sugar binding appears to be due to suppression of the photoinduced electron transfer (PET) from the pyrene donor to the trigonal arylboronic acid acceptor.
Development of analytical methods based on specificity of interfaces and their structural and kinetic stulies

TERAMAE Norio, NISHIZAWA Seiichi, UCHIDA Tatsuya

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: TOHOKU UNIVERSITY

1999 - 2001

More details Close

As to analytical reaction fields, solid-liquid interface, liquid-liquid interface, meso-porous channels of silcate material, gold and semiconductor nano particles are investigated to obtain highly selective and/or specific recognition of molecules and ions using hydrogen bonding artificail receptors. Gold nano-particles modified with thiolated compounds which have molecule or ion recognition sites. The chemically modified gold particles show dramatic color change from red to blue by addition of target molecules or ions in water. Nano-sized semiconductor particles were also modified by thiolated carboxylic compounds, and sensing lead ions was succesfully attained based on photo-induced electron transfer mechanism. Highly selective sensing of very hydrophilic anions was succeeded using liquid-liquid interfaces as recognition fields. Ion-pair complexation behaviors of a thiourea-based bifunctional receptor with alkali metal ions and anions are examined at the 1,2-dichloroethane-water interface by dynamic interfacial tensiometry. A decrease in the interfacial tension, caused by the interfacial adsorption of the receptor in the organic phase, is promoted when alkali metal salts are added in the aqueous phase. The adsorption constants of the receptor depend on alkali metal salts. The results indicate that hydrogen bonding formation between the receptor and anions is actually effective at the liquid-liquid interface despite the significant interference from anion hydration. A more important property of the receptor at the liquid-liquid interface is the preferential binding of dihydrogen phosephate over chloride. This kind of selectivity has merely observed in ionophore-based chemical sensors such as ion-selective electrodes, where the selectivity is governed predominantly by the order of the free energy of hydration of the analyte anions. Anion transfer from aqueous phase to water phase was also investigated by liquid droplet polarography, A hydrogen bond-forming bis-thiourea was able to effectively facilitate the transfer of various hydrophilic anions across the nitrobenzene-water interface, suggesting possible use of the receptor for multianalyte detection with an amperometric sensing mode. In addition, chloride transfer across the nitrobenzene-water interface facilitated by the ionophore was studied, and a well-defined wave could be observed, indicating that the chloride transfer across the interface was facilitated by the ionophore via formation of hydrogen bonds. The analysis of ion transfer polarograms showed that the chloride transfer was assisted by 1 : 1 complexation between 2 and chloride, and the transfer process was reversible and controlled by diffusion of the ionophore from the bulk organic phase to the interface. Despite its simple binding mode based on two point interactions, mono-thiourea showed a strong ability to facilitate chloride transfer compared with a ionophore in which four hydrogen bonds were involved in the chloride binding. Dyanamical studies were also carried out by laser spectroscopies.
分子内エキサイプレックス発光制御に基づく新規イオン認識蛍光プローブの開発

西沢精一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 奨励研究(A)

Institution: 東北大学

1999 - 2000

More details Close

本研究では,光誘起電子移動(Photoinduced Electron Transfer)反応を利用した,いわゆるPET型と呼ばれる蛍光プローブの新たな展開として,PETにおける電荷移動中間体であるエキサイプレックス(EX)に着目し,分子内EX発光制御に基づくアルカリ金属イオン認識蛍光プローブの開発を行った。蛍光団としてピレン,金属イオン認識部位としてアミドベンゾ15-クラウン-5を採用し,また,両者を繋ぐスペーサーとして長さの異なるアルキル鎖(-C_nH_<2n>-,n=0,1,3,5)から構成される一連の化合物を合成した。種々の有機溶媒中における蛍光スペクトルを測定したところ,EX発光の量子収率がスペーサー長に大きく依存することを見い出した。すなわち,EX発光の量子収率はn=3の場合に最大となり,n=3>n=5>n=1の順に減少する。また,形成するEXのコンフォメーションもスペーサー長に依存し,n=3ではface-to-face型,n=1ではface-to-edge型のEXを形成することが分かった。EX発光の量子収率が最大となるn=3の化合物について,アルカリ金属イオン添加に伴う蛍光スペクトル変化を測定したところ,等発光点を伴ってEX発光が減少し,モノマー発光が増大することを見い出した。これはアルカリ金属イオンとの錯形成によりクラウン部位の電子供与性が低下した結果であると考えられ,EX形成をイオン認識により制御できることを意味する。従来のPET型蛍光プローブが単一蛍光強度変化のみを利用しているのに対し,本研究で開発した蛍光プローブではモノマー/EX発光の強度比が変化するため,分析化学的に有用な2波長強度比解析が可能である。また,同様な設計指針により中性・アニオン性基質に対する2波長解析蛍光プローブ開発への展開も期待できることから,本研究で得られた結果は,今後のEX発光性蛍光プローブ開発において重要な基礎知見と成り得ると言える。
Development and Application of the Three Dimensional Microscope System for Nano Space Spectroscopy

TERAMAE Norio, NISHIZAWA Seiichi, UCHIDA Tatsuya

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B).

Institution: TOHOKU UNIVERSITY

1999 - 2000

More details Close

In order to achieve three-dimensional chemical analyses at submicrometer spatial resolution, a new confocal Raman/fluorescent microscope coupled with close loop piezoelectric actuators for XY scanning of a sample stage and Z scanning of an objective has been developed. The closed loop operation based on reference signals from position sensors attached to the actuators provides good linearity, long time stability and high accuracy of sample displacement. Scattered light or emission from the sample is collected using the objective, and guided to a pinhole and a slit of spectrometer. Performance of this system within XY plane was checked using fluorescent microspheres and phototaic crystals made of silicon. A fluorescence image of two 200 nm spheres aggregating on a quartz glass shows that tops of both spheres are clearly resolved. Furthermore, a Raman image of the photonic crystal collected in the 500 cm-1 band of silicon shows the submicormeter holes arranged triangularly. As mentioned above, the microscope system has been developed successfully.
金属錯体を認識部位に用いる界面集積分子のセンシング機能

早下隆士, 西沢精一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 特定領域研究(A)

Institution: 東北大学

1999 - 1999

More details Close

本研究では,界面での錯形成を利用した分子認識を行うための基礎研究として,まず錯形成部位にクラウンエーテルを用いた蛍光および比色プローブを合成し,その機能解析を行なった。まず水中においてホスト-ゲスト相互作用を促進する疎水環境を有するシクロデキストリンを利用することにより,これに包接されたクラウンエーテル型蛍光プローブが水中において高度にアルカリ金属イオンを認識できる系を開発した。ピレン官能基を有するクラウンエーテル型プローブ/γ-シクロデキストリン複合体を用いて,アルカリ金属イオン認識能を評価した。蛍光分光法により解析した結果,その蛍光スペクトルは,Na^+塩の添加により470nm付近に新たな発光を示すことを見出した。イオン半径とクラウンエーテルの空孔サイズから,長波長側の発光は,金属イオンとの2:1錯体形成によるピレン二量体の蛍光であることがわかった。この系を用いて,水中でのK^+の高感度センシング達成した。次に第二高調波発生 (SHG) 分光法を用いて吸着分子の電子状態や配向性,密度に関する解析を行なうことで、液液界面におけるクラウンエーテル型アゾプローブのアルカリ金属イオンを設定し、SHG分光計測を行なったところ,600nm付近ではSHGが全く観測されないのに対し,600nm以下では吸着分子の吸収帯との共鳴によるSHG強度の増大が観測された。これによって,界面における選択性は,液液抽出選択性と一致することを明らかにすると共に,入射偏光依存性の解析から界面での認識に基づくプローブの配向変化を直接観察することに成功した。
ナノポアを有するシリカ多孔体を用いた分析反応場の設計と応用

寺前紀夫, 西沢精一, 内田達也

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 萌芽的研究

Institution: 東北大学

1998 - 1998

More details Close

界面活性剤の棒状ミセルを鋳型として合成されたシリカ系物質(MCM-41)がハニカム状でナノメートルサイズの均一な孔径を持つことから,その開発以来,触媒化学の分野を主体として活発な研究が始められている。一方,分析化学的立場からはモルキュラーシーブやカラム分離剤,吸着剤などの直接的応用も期待されるが,細孔が一次元性の構造を持つことに着目した新規物質の合成も重要である。分析化学における新たな標準試料の調製手段としての利用,およびナノメートル領域を評価する顕微分析法へ適用をめざして,ナノメートルサイズにおける物質の構造制御を行うことを研究目的とした。 MCM-41の細孔内部のシラノール基にイオン交換を利用してCuまたはZnを導入し,これを鋳型としてジシアノベンゼンの気相蒸着を行い,ナノ細孔内での金属フタロシアニンのShip-in-a-Bottle合成に成功した。反応量変化に伴う拡散反射スペクトル変化を検討した結果,生成量増大に伴うQバンドの励起子分裂の解析から会合状態の変化が分かり,高充填では一次元会合体の生成が示唆された。また,細孔内へ蛍光性分子(ペリレン)を気相導入し,高分子マトリックス中にスピンコートにより分散させ,その単一粒子について共焦点蛍光顕微計測を行った。蛍光スペクトルには高充填の場合,エキシマーに由来する発光帯が観測され,ペリレン分子の会合が確認された。また,各粒子から発生する蛍光強度に励起偏光依存性,すなわち異方性が観測されたことから,細孔内でペリレン分子集合体が配向していることが分かった。以上の研究により,一次元ナノ細孔内における分子合成ならびに配向制御の有効性を明確化した。
多点分子認識に伴うエキシマー発光を利用したアニオン蛍光プローブの開発

西沢精一

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 奨励研究(A)

Institution: 東北大学

1997 - 1998

More details Close

本研究では,生体関連アニオンに対する新規蛍光プローブの開発を目的として以下の研究を行った。 PET(photoinduced electron transfer)センサーの新たな展開として,分子内エキサイプレックス(EX)形成制御に基づく新規蛍光プローブの開発を試みた。アニオン認識部位としてチオ尿素基を有するピレン誘導体を新規合成し,有機溶媒中における錯形成能を評価した。その結果,CH_3'CO_2^-やH_2PO_4^-等と水素結合を介して1:1錯体を形成することにより,モノマー蛍光が著しく消光し,その長波長側にEX発光を与えることを明らかにした。従って,アニオンとの錯形成をEX/モノマー発光強度比の変化によりモニターすることが可能であり,また,EX発光のλ_<max>はアニオン種に依存する。分析試薬として有用な,これらのスペクトル応答は,従来のPETセンサーでは殆ど達成し得なかったものであり,分子内EX発光を利用したイオンセンシングの優れた可能性を示唆している。自己集合体形成に基づく新規蛍光プローブの開発を試みた。アニオン認識部位としてグアニジニウム基を有するビレン誘導体を新規合成し,メタノール中における錯形成能を評価した。その結果,ピロリン酸イオン(PPi)との2:1錯体形成を,モノマー/エキシマー発光強度比変化として検出可能であることを明らかにした。すなわち,PPi添加に伴い,モノマー発光は消光し,その長波長側に顕著なエキシマー発光が見られる。このような蛍光スペクトル応答は,他のアニオン(H_2PO_4^--,CH_3CO_2^-,Cl^-,Br^-)では全く見られず,PPiに対して高選択的である。以上,従来の蛍光試薬の殆どが単にアニオンとの消光作用や蛍光物質への誘導体化に基づいているのとは全く異なり,エキシマーあるいはエキサイプレックス発光を利用することにより,新しい情報変換モードに基づくアニオン認識蛍光プローブを開発することに成功した。
Molecular Design of Alkali Metal Ion Probes for Use in Aqueous Media

HAYASHITA Takashi, TAKAGI Makoto, NISHIZAWA Seiichi, UCHIDA Tatsuya

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

1997 - 1998

More details Close

A selective colorimetry of alkali metal cations in aqueous micellar solution containingsym-(alkyyl)(2-hydroxy-5-nitrobenzyloxy)dibenzo-1 6-crown-5 (1) has been examined. Colorimetric behavior were found to be strongly affected by the surface charge of micellar species and a selective colorimetry of Na^+ was performed in the anionic micellar solution containing lipophilic DB 16C5 chromoionophore. By introducing pyrenecarboxamide unit into DB16C5 skeleton (2), a fluorescent response for Na^+ binding is expected. It was found that proton dissociation of the carboxamido moiety in fluoroionophore was promoted by Na^+ binding, which resulted in an efficient ratiometric emission due to internal charge transfer (ICT) from the donorcarboxamido anion to the pyrene acceptor. No fluorescent response was induced by the presence of Li^+ or Cs^+. Thus fluoroionophore 2 exhibited a dual emission response with high Na^+ selectivity in 4 : 1 1 , 4-dioxane-water (v/v). The benzo-15-crown-5 (B15C5) fluoroionophore 3 can be classified as a photoinduced electron transfer (PET) sensor. A moderate Na^+ selectivity is obtained for 3 based on a 1 : 1 complex formation ability of the B 1 5C5 moiety in a non-aqueoussolution. In contrast, 3 was found to work as a novel ion probe in the presence of gamma-CyD, and it achieved high sensitivity and selectivity for K^+ even in the presence of Na^+. The results revealed that 3 formed a 2 : 1 complex with high selectivity for K^+ in the presence of gamma-CyD, and it exhibited both monomer and dimer emission in water.
Development of Molecular Recognition Reaction at the Interface and Analysis of Recognition Functions by Laser Spectroscopy

TERAMAE Norio, NISHIZAWA Seiichi, UCHIDA Tatsuya, HAYASHITA Takashi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: TOHOKU UNIVERSITY

1997 - 1998

More details Close

As to molecular recognitions at the interface, hydrogen bonding receptors were synthesized and their functions were evaluated, paying attentions to recognition functions used in living systems. Reactivities and adsorption behaviors of molecules at the interface were analyzed by resonant second harmonic(RSH) spectroscopy, time resolved total internal reflection fluorescence spectroscopy(TIRF), electrochemical STM, QCM, dynamic interfacial tensiometry, and liquid droplet polarographic measurements. As a new generation of fluoroionophores based on the photoinduced electron transfer process, novel pyrene-based receptors which are donor-spacer-acceptor systems were developed. A fluorophore as an electron acceptor, pyrene, was linked to an ion receptor as an electron donor by a polymethylene chain. In these systems, a formation of an intramolecular exciplex between the excited pyrene and the ion receptor is controlled by ion complexation, resulting in the spectral responses to allow analytically useful ratiometric measurements, that is, a ratio of the exciplex emissin to the monomer emission can be used to cancel out the environmental effect. In a pyrene/thiourea system, where the thiourea group functions as an anion binding site, the maxima of the exciplex emission enhanced by anion binding were foamed to be clearly anion dependent. This dependency is another advantage of the exploitation of the exciplex emission for ion sensing. In a pyrene/amidobenzo-15-crown-5 system, where the amidobenzo-15-crown-5 group functions as an cation binding site, the fluorescence maximum of the exciplex emission was red-shifted with increasing solvent polarity, indicating the charge-transfer nature of the emission. The intensity of the emission band decreased by adding alkali metal ions. This decrease reflects the stabilities of the 1 : 1 complex. As for molecular recognitions at liquid-Liquid interfaces, complexation reactions of hydrogen-bonding reveptors with anions were examined by electrolyte ascending electrode polarography, and the facilitated aniontransfer across the interface was observed for the first time. It was found that sulfate was remarkably facilitated regardless its hydrophilic nature. As mentioned above, several ionophores were developed and examined their functions. Some of the ionophores were demonstrated to recognize anions effectively through hydrogen-bond at liwuid-liquid interfaces.
電気活性人工アニオンレセプターを利用したリン酸イオンセンサーの開発

寺前紀夫, 西沢精一, 内田達也

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 重点領域研究

Institution: 東北大学

1997 - 1997

More details Close

電気活性レセプターは、目的イオンを認識する錯形成部位と、フェロセン等のレドックス中心から構成されており、目的イオンとの錯形成をレドックス中心の酸化還元電位の変化として取り出すことができるため、新規センシングデバイスとしての可能性が近年期待されている。従来、アルカリ金属イオン等のカチオンに対する電気活性レセプターは多数の報告例が、リン酸イオン等の単純な無機アニオンを電気化学的に認識する人工レセプターの報告例は極めて限られている。特に電極表面への固定化による単分子層修飾電極の報告例はなく、本研究により新たな分野が展開されると期待できる。本研究では、まず、1)無機リン酸イオンに対する電気活性人工アニオンレセプターの開発を試みる。更に、2)新規レセプターを電極表面上に固定・配列させることにより単分子層修飾電極を作製し、新規センシングデバイスの構築を行うと共に、3)電極/(水)溶液界面における分子認識過程の基礎的理解を得ることを目的とする。本年度の研究成果を要約すると以下のようになる。アニオン認識サイトとしてアミド、尿素あるいはチオ尿素部位、レドックス中心としてフェロセンから構成される化合物を新規合成し、有機溶媒中におけるアニオンとの錯形成能および錯形成に伴う電気化学特性変化について検討した。その結果、H_2PO_4^-やCl^-と水素結合を介して錯形成することにより、酸化ピーク電位がネガティブシフトすることを見出した。例えば、尿素部位を認識サイトとするレセプターのアセトニトリル溶液にアニオン(対イオン:(C_4H_9)_4N^+)を1等量添加した際のシフト値は、H_2PO_4^-(30mV)>Cl^-(15mV)であり、錯形成の序列(K_<11>(M^<-1>)in CD_3CN:H_2PO_4^-:360;Cl^-:200)とよく一致する。以上、水素結合によるアニオン認識に基づく新規電気活性人工アニオンレセプターの開発に成功した。
Development of In-situ Non-lenear Spectroscopic Probe microscope and its Application to the Surface Phenomena

TERAMAE Norio, SUGIMURA Hiroyuki, NISHIZAWA Seiichi, UCHIDA Tatsuya

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: TOHOKU UNIVERSITY

1995 - 1996

More details Close

Chemical and physical processes occured at solid-liquid interfaces have been paid attentions from the fundamental and technological points of view. In recent years, scanning probe microscopes and non-linear surface spectroscopies have began to reveal new aspects for the interfacial phenomena. In the present study, chemical reactions occured at electrode surfaces were studied at a molecular level by scanning tunnel microscope (STM) and non-linear laser spectroscopies such as second harmonic generation and sum frequency generation (SFG). In the electrochemical STM study, several preparation methods were examined for obtaining an optimum tip which can be used for the measurements of STM and cyclic voltammetry. The tips prepared were also evaluated by SEM.An electrode was immersed in an aqueous solution of Fe (III) protoporphyrin (Fe-PP), and then transferred to an electrochemical cell after rinsing with distillled water. STM meaaurements were carried out in a buffer solution (pH 10). It was found that Fe-PP absorbed on a graphite electrode showed multi-layred structure when the amount of adsorption is rich, and that the well-ordered structure having a regular lattice constant was observed for each adlayr. In contrast to this observation, Fe-PP was found to form a linear chain when the amount of adsorbed molecules is poor. These observations clarified the chemical process occured at the electrode surface, that is, the physical structures of Fe-PP depend on the coverage. To obtain chemical structure of adsorbate, resonant second harmonic spectroscopy was applied to the analysis of redox behaviors of methylene blue at the sulfer coated electrode surface. As a result, dependence of amount of adsorbate on the orientation and electronic structure could be discussed. As for SFG,measurement system was developped based on a pulsed Nd : YAG laser with a differential frequency generator, and fundamental factors which affect the measurements were examined. Although spectral resolution was not enough high, vibrational spectra of octadecanetiol on Au (111) electrode could be measured with a resolution of ca 20 cm^<-1>. As mentioned above, chemical processes occured at solid-liquid interfaces could be analyzed at a molecular level by combining the electrochemical STM with surface sensitive non-linear laser spectroscopies.
Measurements of Non-Equilibrium State for Reactions at Liquid-Liquid Interfaces and the Analysis of Selective Distribution Behaviors

TERAMAE Norio, NISHIZAWA Seiichi, UCHIDA Tatsuya, SAITOH Koichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: TOHOKU UNIVERSITY

1994 - 1996

More details Close

As measurement methods for reactions at liquid-liquid interfaces, the resonat second harmonic (RSH) spectroscopy and the time resolved total internal reflection fluorescence spectroscopy (TIRF) were developped and applied to characterize molecules absorbed at liquid-liquid interfaces. In addition, the potential-scan polarography with an electrolyte dropping electrode was developped for the analysis of the complexation kinetics at liquid-liquid interfaces. As for RSH spectroscopy, rhodamine B (Rh-B) is studied as a moleule adsorbed at liquid-liquid interfaces. Rh-B molecules were found to be stabilized at the heptane-water interface compared bulk solutions. Based on the concentration dependence of RSH spectra, it was found that molecules at the interface can exist as a mixture of monomer and dimer when the bulk concentration is high. Molecular orientations of Rh-B were estimated and the orientation angle was almost constant as 65 degree when the concentration is low, and the angle decreased at higher concentrations. A time resolved TIRF was applied to study the microenvironments of 8-anilino-1-naphthalenesulfonate molecules at the heptane-water interface. By analysing the fluorescence decay profiles, it was found that the fluorophores in the vicinity of the interface are present in two different solvated structures. One of them is located up to a few nanometers from the interface, and the other is located in a relatively wide range twoard the aqueous phase. The former solvated structure is the cause of a long fluorescence lifetime which reflects the low polarity or the extremely high viscosity of the microenvironments. As for chemical reactions at liquid-liquid interfaces, complexation kinetics were examined using 8-quinolinol derivatives and divalent metal ions by measuring dynamic interfacial tension and ion transfer across the interface. As a result, it was found that 5-octyloxymethyl-8-quinolinol showed interface absorptivity and the adorptivity depended on both the counter anion and the organic phase. These dependence was explained by the ion pair formation constant and the distribution coefficient of a chelating reagent. From the measurement of an ion tranfer across the interface, it was suggested that the complexations occured at the interface nearer to the organic phase. As mentioned above, several methods were establishied to analyze reactions at liquid-liquid interfaces, and the reactions at the interface were found to be different from those in bluk solutions.

Show all Show first 5

Social Activities 8

ぶらりがく「ホタルの光を化学する！？」

2016/12/16 -

More details Close

科学イベント
茨城県立竜ヶ崎第一高等学校「大学・学部・学科研究会」

2016/06/23 -

More details Close

出前授業
第37回教師のための化学教育講座

2014/08/11 -

More details Close

公開講座「小さな有機分子による遺伝子分析」
磐城高等学校理学部訪問研修

2014/04/24 -

More details Close

理学部紹介と体験講義
山形県立山形中央高等学校「2学年大学説明会」

2013/12/11 -

More details Close

「ホタルの光と化学発光」と題して、出前授業をおこなった。
コスモス理科実験講座（宮城県宮城第一高等学校）

2009/08/05 -

More details Close

化学発光蛍の光と血痕の検出
第８回東北大学出前授業（仙台市立折立中学校）

2008/11/13 -

More details Close

「蛍の光と血液の検出：化学発光を楽しもう」
岩手県立盛岡第一高等学校出張講義

2008/11/12 -

More details Close

「“蛍の光”と血痕の検出：化学発光」

Show all Show first 5

Other 1

生体分子の高次構造形成に基づく遺伝子診断法

More details Close

「DNAの高次構造形成と有機小分子プローブを利用する、一塩基多型（SNPs）検出法の開発」を目的とする。