Details of the Researcher

PHOTO

Kirika Ishiyama
Section
Graduate School of Dentistry
Job title
Assistant Professor
Degree

  • (Tohoku University)

Papers 12

  1. Bactericidal activity and recovery effect of hydroxyl radicals generated by ultraviolet irradiation and silver ion application on an infected titanium surface International-journal Peer-reviewed

    Taichi Tenkumo, Kirika Ishiyama, Oleg Prymak, Keisuke Nakamura, Midori Shirato, Toru Ogawa, Makiko Miyashita, Masatoshi Takahashi, Matthias Epple, Taro Kanno, Keiichi Sasaki

    Scientific Reports　10　(1)　8553-8553　2020/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-020-65411-4 　

    eISSN： 2045-2322

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    This study investigated the bactericidal effect, the underlying mechanisms of treatment, and recovery of biocompatibility of the infected titanium surface using a combination treatment of silver ion application and ultraviolet-A (UV-A) light irradiation. Streptococcus mutans and Aggregatibacter actinomycetemcomitans were used in suspension and as a biofilm on a titanium surface to test for the bactericidal effect. The bactericidal effect of the combination treatment was significantly higher than that of silver ion application or UV-A light irradiation alone. The bactericidal effect of the combination treatment was attributable to hydroxyl radicals, which generated from the bacterial cell wall and whose yield increased with the silver concentration. To assess the biocompatibility, proliferation and calcification of MC3T3E1 cells were evaluated on the treated titanium surface. The treated titanium screws were implanted into rat tibias and the removal torques were measured 28 days post-surgery. The titanium surface that underwent the combination treatment exhibited recovery of biocompatibility by allowing cellular proliferation or calcification at levels observed in the non-infected titanium surfaces. The removal torque 28 days after surgery was also comparable to the control values. This approach is a novel treatment option for peri-implantitis.

  2. Oral mucosal irritation potential of antimicrobial chemotherapy involving hydrogen peroxide photolysis with high-power laser irradiation for the treatment of periodontitis International-journal Peer-reviewed

    Midori Shirato, Keisuke Nakamura, Taichi Tenkumo, Yuki Kano, Kirika Ishiyama, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano, Hiroshi Matsuura

    Journal of Photochemistry and Photobiology B: Biology　201　111633-111633　2019/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.jphotobiol.2019.111633 　

    ISSN： 1011-1344

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    In the present study, we assessed the oral mucosal irritation potential of antimicrobial chemotherapy involving hydrogen peroxide (H2O2) photolysis with a 405-nm laser device at an output power of ≥100 mW in hamsters. Twenty-four cheek pouches from 12 male Syrian hamsters received 7-min treatment with pure water (PW), 3% H2O2, laser irradiation of PW at 100 mW, laser irradiation of 3% H2O2 at 100 mW, laser irradiation of PW at 200 mW, or laser irradiation of 3% H2O2 at 200 mW (n = 4 each). The diameter of the irradiation area was set at 3 mm; accordingly, the calculated irradiances (optical power densities) of the 100- and 200-mW laser lights were approximately 1400 and 2800 mW/cm2, respectively. In addition, 12 cheek pouches from six animals received laser irradiation of 3% H2O2 at 100 mW for 1, 3, or 5 min (n = 4 each). Each treatment was repeated three times at 1-h intervals. Macroscopic and histological changes were evaluated 24 h after the last treatment. In addition, in vitro bactericidal activity of the treatment against periodontal pathogens was evaluated. We found that 405-nm laser irradiation of 3% H2O2 caused moderate to severe oral mucosal irritation when performed at powers of 100 and 200 mW for ≥3 min, while the same treatment performed at 100 mW for 1 min resulted in mild irritation. Moreover, 1-min H2O2 photolysis at 100 mW caused a >4-log decrease in viable bacterial counts. These findings suggest that 1-min H2O2 photolysis, which can effectively kill periodontal pathogens, may be acceptable when a 405-nm laser device is used at 100 mW. However, use of the laser at a lower power would be preferable for the prevention of unnecessary oral mucosal irritation.

  3. 医師主導治験におけるラジカル殺菌歯周病治療器の臨床効果の検証 無作為化比較試験 Peer-reviewed

    佐々木 啓一, 菅野 太郎, 中村 圭祐, 石山 希里香, 山田 康友, 白土 翠, 庭野 吉己, 萱場 千恵, 池田 浩治, 高木 愛理, 山口 拓洋

    日本歯周病学会会誌　61　(春季特別)　127-127　2019/05

    Publisher: (NPO)日本歯周病学会

    ISSN： 0385-0110

    eISSN： 1880-408X

  4. Potential adverse effects of antimicrobial chemotherapy based on ultraviolet-A irradiation of polyphenols against the oral mucosa in hamsters and wounded skin in rats International-journal Peer-reviewed

    Keisuke Nakamura, Taichi Tenkumo, Takayuki Mokudai, Midori Shirato, Kirika Ishiyama, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano

    Journal of Photochemistry and Photobiology B: Biology　187　96-105　2018/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.jphotobiol.2018.08.006 　

    ISSN： 1011-1344

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    Antimicrobial chemotherapy based on ultraviolet-A (UVA) irradiation of polyphenol solution has been proposed as an adjunctive treatment for dental caries. However, the safety of this treatment has not been thoroughly evaluated. Therefore, the aim of the present study was to assess the influence of this treatment on the oral mucosa in hamsters and wounded skin in rats. An oral mucosal irritation test was performed in hamsters. The cheek pouch was everted and treated with UVA irradiation (wavelength: 365 nm) of pure water, 1 mg/mL of caffeic acid, or 1 mg/mL of grape seed extract using a light-emitting diode at an irradiance of 275 mW/cm2. Each treatment was performed for 2 or 5 min and repeated three times. Macroscopic and histological evaluations were performed 24 h after the last treatment. We also examined the effects of the treatment on the healing process of skin wounds in rats. Full-thickness skin wounds created on the back of the rats were treated for 2 min as described above, but only once. The wound area was then assessed daily for 9 days. The results demonstrated that the treatment induced oral mucosal irritation depending on the irradiation time; however, it did not influence the wound healing process. The oral mucosal irritation potential of three treatment sessions performed for 2 and 5 min was minimal and mild to moderate, respectively, according to histological analysis. These findings suggest that the duration of treatment based on UVA irradiation of polyphenols in the oral cavity should be as short as possible, considering the clinical efficacy of the antimicrobial effects and the irritation potential.

  5. Proanthocyanidin-Rich Grape Seed Extract Modulates Intestinal Microbiota in Ovariectomized Mice Peer-reviewed

    Guangwen Jin, Yoshinori Asou, Kirika Ishiyama, Atsushi Okawa, Taro Kanno, Yoshimi Niwano

    Journal of Food Science　83　(4)　1149-1152　2018/04

    Publisher: Wiley

    DOI： 10.1111/1750-3841.14098 　

    ISSN： 0022-1147

  6. Proanthocyanidin-rich grape seed extract prevent estrogen deficiency-induced metabolic disorders Peer-reviewed

    Guangwen Jin, Alkebaier Aobulikasimu, Jinying Piao, Zulipiya Aibibula, Daisuke Koga, Hiroki Ochi, Kirika Ishiyama, Taro Kanno, Yoshimi Niwano, Atsushi Okawa, Yoshinori Asou

    Journal of Medical and Dental Sciences　65　(2)　45-50　2018

    Publisher: Tokyo Medical and Dental University

    DOI： 10.11480/jmds.650201 　

    ISSN： 2185-9132 1342-8810

  7. Adjunctive antimicrobial chemotherapy based on hydrogen peroxide photolysis for non-surgical treatment of moderate to severe periodontitis: a randomized controlled trial Peer-reviewed

    Taro Kanno, Keisuke Nakamura, Kirika Ishiyama, Yasutomo Yamada, Midori Shirato, Yoshimi Niwano, Chie Kayaba, Koji Ikeda, Airi Takagi, Takuhiro Yamaguchi, Keiichi Sasaki

    Scientific Reports　7　(1)　2017/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-017-12514-0 　

    eISSN： 2045-2322

  8. Bactericidal Action of Photodynamic Antimicrobial Chemotherapy (PACT) with Photosensitizers Used as Plaque-Disclosing Agents against Experimental Biofilm Peer-reviewed

    KIRIKA ISHIYAMA, KEISUKE NAKAMURA, TARO KANNO, YOSHIMI NIWANO

    Biocontrol Science　21　(3)　187-191　2016

    Publisher: The Society for Antibacterial and Antifungal Agents, Japan

    DOI： 10.4265/bio.21.187 　

    ISSN： 1342-4815

    eISSN： 1884-0205

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    <p>Our previous study revealed that the photo-irradiation of rose bengal, erythrosine, and phloxine, xanthene photosensitizers, used as dental plaque disclosing agents, could exert bactericidal action on planktonic Streptococcus mutans via the singlet oxygen. In the present study, the photo-irradiation induced bactericidal activity of the three xanthene compounds against the experimental biofilm of S. mutans was investigated in combination with acid electrolyzed water (AcEW) and alkaline electrolyzed water (AlEW). As a result, only the photo-irradiated rose bengal in AlEW showed prominent bactericidal activity with a >3-log reduction of the viable bacterial count. Since our previous study showed that the affinity of rose bengal to bacterial cells was superior to that of erythrosine and phloxine, it was speculated that AlEW damaged the extracellular matrix of the experimental biofilm, which would let the rose bengal easily be bound to the bacterial cells. From these results, it is strongly suggested that rose bengal is a suitable photosensitizer for use as a plaque disclosing agent in photodynamic antimicrobial chemotherapy to treat dental plaque.</p>

  9. Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria Peer-reviewed

    Keisuke Nakamura, Kirika Ishiyama, Hong Sheng, Hiroyo Ikai, Taro Kanno, Yoshimi Niwano

    Journal of Agricultural and Food Chemistry　63　(35)　7707-7713　2015/09/09

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/jf5058588 　

    ISSN： 0021-8561

    eISSN： 1520-5118

  10. 細菌、ウィルスに対するPDTの研究成果 歯垢染色剤に含まれる各種キサンテン系色素を光感受性物質とした一重項酸素依存性光線力学殺菌療法の比較 Peer-reviewed

    庭野 吉己, 石山 希里香, 中村 圭祐, 猪飼 紘代, 菅野 太郎, 佐々木 啓一

    日本レーザー医学会誌　34　(3)　326-326　2013/10

    Publisher: (NPO)日本レーザー医学会

    ISSN： 0288-6200

    eISSN： 1881-1639

  11. Bactericidal Action of Photogenerated Singlet Oxygen from Photosensitizers Used in Plaque Disclosing Agents Peer-reviewed

    Kirika Ishiyama, Keisuke Nakamura, Hiroyo Ikai, Taro Kanno, Masahiro Kohno, Keiichi Sasaki, Yoshimi Niwano

    PLoS ONE　7　(5)　e37871-e37871　2012/05/22

    Publisher: Public Library of Science (PLoS)

    DOI： 10.1371/journal.pone.0037871 　

    eISSN： 1932-6203

  12. Reevaluation of analytical methods for photogenerated singlet oxygen Peer-reviewed

    Keisuke Nakamura, Kirika Ishiyama, Hiroyo Ikai, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano, Masahiro Kohno

    Journal of Clinical Biochemistry and Nutrition　49　(2)　87-95　2011

    Publisher: The Society for Free Radical Research Japan

    DOI： 10.3164/jcbn.10-125 　

    ISSN： 0912-0009

    eISSN： 1880-5086

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Misc. 2

  1. カフェイン酸に対するUV-A照射で得られる殺菌作用と口腔粘膜刺激性の評価

    中村圭祐, 天雲太一, 目代貴之, 白土翠, 石山希里香, 菅野太郎, 佐々木啓一, 庭野吉己

    日本防菌防黴学会年次大会要旨集　45th　2018

  2. Comparison of different analytical methods for determining singlet oxygen photogenerated from rose bengal

    Kirika Ishiyama, Keisuke Nakamura, Hiroyo Ikai, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano, Masahiro Kohno

    Interface Oral Health Science 2011　89-91　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_15 　

Research Projects 5

  1. The hydrogen peroxide photolysis treatment could be a novel approch for refractory endodontic disease

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2024/04/01 - 2027/03/31

  2. Facilitation of wound healing by proanthocyanidin: Application in gerodontology

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2021/04 - 2024/03

  3. リン酸カルシウム遺伝子導入剤による機能性抗菌コーティングのインプラント周囲炎応用

    天雲 太一, 石山 希里香, 中村 圭祐, 白土 翠

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04/01 - 2023/03/31

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    インプラント治療は表面性状の発展に伴い治療の成功率が飛躍的に向上することで、世界的に普及する治療方法となった半面、インプラント周囲炎の報告も増加している。しかし、インプラント周囲炎に対する有効な治療方法はまだないことが歯科治療において大きな問題となっている。インプラント治療では、細菌などで汚染した表面の殺菌療法だけでは不十分で、その後に骨の再付着を誘導するようなシステムの確立が重要となる。そこで、本研究ではラジカル殺菌処置後に抗菌性リン酸カルシウム遺伝子導入ナノ粒子によるコーティング層を構築することで、再感染予防と再オステオインテグレーションの獲得をする新しいインプラント周囲組織再生療法を確立することを目的としている。まず、「リン酸カルシウムコーティング層の作製条件」を検証したところ、照射強度や照射時間は一定量を超えると、リン酸カルシウムの粒子の付着量に変化がなくなり、上限があることが明らかにしてきた。また、殺菌効果については、同じ照射強度、照射時間でも対象となる細菌の濃度には適正濃度があること、その適正濃度は細菌の種類によって異なることが明らかとなった。一方、「CaP コーティング層の表面性状、生体親和性」について解析したところ、菌種によってはリン酸カルシウム自体が細菌の増殖を助長していることが確認された。そこで、新たにこれまでと異なる抗菌ペプチドを付与したリン酸カルシウムナノ粒子を製作し、同細菌に対して殺菌試験を行ったところ、リン酸カルシウム自体による細菌増殖効果が抑制されることが明らかとなった。現在は同リン酸カルシウムナノ粒子の遺伝子導入効率や生体親和性をインプラント周囲に存在する歯周組織関連細胞を用いて検証している。

  4. developmengt of novel peri-implantitis treatment using hydroxyl radical- effect of combination with ultraviolet irardiation and silver ions

    Ishiyama Kirika

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Category: Grant-in-Aid for Early-Career Scientists

    Institution: Tohoku University

    2018/04 - 2021/03

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    This study investigated the bactericidal effect, the underlying mechanisms of treatment, and recovery of biocompatibility of the infected titanium surface using a combination treatment of silver ion application and ultraviolet-A (UV-A) light irradiation. The bactericidal effect of the combination treatment was significantly higher than that of silver ion application or UV-A light irradiation alone. The bactericidal effect of the combination treatment was attributable to hydroxyl radicals. Furthermore, the titanium surface that underwent the combination treatment exhibited recovery of biocompatibility by allowing cellular proliferation or calcification at levels observed in the non-infected titanium surfaces and the removal torque was also comparable to the control values. From these results, this approach is a novel treatment option for peri-implantitis.

  5. Bactericidal action of photodynamic antimicrobial chemotherapy (PACT) with photosensitizers used as plaque disclosing agents

    Ishiyama Kirika

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2015/04 - 2018/03

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    The present study demonstrated that photodynamic antimicrobial chemotherapy (PACT) using rose bengal as a photosensitizer was effective against planktonic bacteria. However, the antimicrobial activity against biofilm was considerably low. In contrast, antimicrobial chemotherapy based on UVA irradiation of caffeic acid aqueous solution showed high bactericidal effect against Streptococcus mutans biofilm. Caffeic acid is a kind of polyphenols and contained in various fruits and vegetables. This indicates that remaining caffeic acid after treatment may not be toxic when this antimicrobial technique is used in oral cavity. In addition, wounded skin irritation test using rats showed that the treatment did not deter the healing process. Therefore, it is suggested that the UVA irradiation of polyphenols be more suitable for dental application than PACT.