Details of the Researcher

PHOTO

Hiromu Tanimoto
Section
Graduate School of Life Sciences
Job title
Professor
Degree

  • Ph. D. (Science) (The University of Tokyo)

Research History 4

  • 2013 - Present
    Tohoku University　Graduate School of Life Sciences　Professor

  • 2008 - 2013
    Max-Planck Institute of Neurobiology　Group Leader

  • 2006 - 2007
    University of Wuerzburg　Department of Genetics and Neurobiology　Group Leader of Emmy-Noether Programme (DFG)

  • 2002 - 2006
    University of Wuerzburg　Department of Genetics and Neurobiology　Postdoctoral fellow

Education 2

  • The University of Tokyo　Graduate School of Sciences (Institute of Molecular and Cellular Biosciences)

    1997 - 2002

  • Tokyo Metropolitan University　Department of Chemistry

    - 1997

Research Interests 1

  • neuroethology

Research Areas 2

  • Life sciences / Animals: biochemistry, physiology, behavioral science /

  • Life sciences / Neuroscience - general /

Awards 2

  1. Thomson Reuters Japan Research Front Awards 2016

    2016/07/07　Thomson Reuters　報酬シグナルとして働くドーパミンニューロン

  2. 第12回（平成27年度）日本学術振興会賞

    2015/12　日本学術振興会　ショウジョウバエ記憶回路の網羅的解析とドーパミン神経機能の解明

Papers 95

  1. Presynaptic computation of reward intensities through the dual autoreceptor system. International-journal

    Kokoro Saito, Shun Hiramatsu, Aoi Watanabe, Hongyang Wu, Toshiharu Ichinose, Nobuhiro Yamagata, Hiromu Tanimoto

    Current Biology　2026/04/23

    DOI： 10.1016/j.cub.2026.03.077 　

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    To make optimal decisions, animals must accurately differentiate reward intensities, yet synaptic mechanisms for this computation remain poorly understood. Here, we show presynaptic gain control of reward signals in the dopaminergic neurons (DANs) of Drosophila melanogaster, mediated by two opposing dopamine autoreceptors, Dop1R1 and Dop2R. Cell-type-specific endogenous protein tagging and functional imaging of the reward-signaling DANs revealed the localization of both receptors at active zones and the regulation of presynaptic calcium in response to distinct reward intensities. Reward learning with cell-type-specific silencing of these receptors uncovered the role of Dop2R in attenuating reward signals specifically at high concentrations of sugar and alcohol, in contrast to selective amplification of low-intensity rewards by Dop1R1. This dose-specific and bidirectional regulation may extend the dynamic range of perceived reward intensity, enabling the selection of options that predict better outcomes.

  2. A bidirectional brain-fat body axis for pathogen avoidance. International-journal

    Yujie Wang, Jean-François De Backer, Aurélie Muria, Ayako Abe, Kokoro Saito, Helen Holvoet, Mareike Selcho, Hiromu Tanimoto, Ilona C Grunwald Kadow

    Neuron　2026/04/16

    DOI： 10.1016/j.neuron.2026.03.026 　

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    Ingesting pathogens poses a threat to survival, driving the evolution of avoidance behaviors across species. The mechanisms linking immune detection to behavioral responses remain poorly understood. Here, we identify a bidirectional fat body-brain communication pathway that mediates pathogen avoidance in Drosophila melanogaster. Immune receptors and a specific antimicrobial peptide (AMP) are required in both the fat body and neuromodulatory neurons to suppress pathogen intake. We show that pathogen-sensing octopaminergic neurons innervate the fat body, activating calcium signaling via an octopamine receptor, thereby triggering fat body dopamine release. Dopamine then acts through Dop1R1 receptors in mushroom body output neurons to drive avoidance behavior. Two-photon calcium imaging reveals that pathogen ingestion modulates odor responses in these neurons, linking immune system activation to behavioral change. Our findings uncover a previously unrecognized immune-to-brain communication loop, illustrating how fat tissue and the innate immune system can drive behavioral adaptation to enhance survival during infection.

  3. Profiling presynaptic scaffolds using split-GFP reconstitution reveals cell-type-specific spatial configurations in the fly brain. International-journal

    Hongyang Wu, Yoh Maekawa, Sayaka Eno, Shu Kondo, Nobuhiro Yamagata, Hiromu Tanimoto

    eLife　14　2026/03/18

    Publisher: eLife Sciences Publications, Ltd

    DOI： 10.7554/eLife.107663 　

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    Abstract Characterization of intracellular synapse heterogeneity aides to understand the intricate computational logic of neuronal circuits. Despite recent advances in connectomics, the spatial patterns of synapses and their inter-individual variability remain largely unknown. Using directed split-GFP reconstitution, we achieved visualization of endogenous Bruchpilot (Brp), a presynaptic active zone (AZ) scaffold protein, in a cell-type-specific manner. By developing a high-throughput quantification pipeline, we profiled AZ structures in identified neurons of the mushroom body circuit, where intracellular synaptic patterns are crucial due to compartmentalized connectivity. Quantitative characterization of the pattern of Brp clusters across multiple individuals revealed cell-type-dependent synapse heterogeneity and stereotypy. Furthermore, we discovered previously unidentified sub-compartmental synapse configuration and its transient structural plasticity triggered by associative learning. Synapse profiling thus uncovered multi-layered organizations of AZs, ranging from neighboring synapses to consistent patterns across individuals.

  4. Disruption of a selective vesicle pool upon retrograde amnesia dissociates memory at presynaptic terminals. International-journal

    Shun Hiramatsu, Kaito Kabetani, Shu Kondo, Hiromu Tanimoto

    Proceedings of the National Academy of Sciences of the United States of America　123　(10)　e2514875123　2026/03/10

    DOI： 10.1073/pnas.2514875123 　

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    A single learning episode induces both labile and consolidated forms of aversive olfactory memory in Drosophila melanogaster. Retrograde amnesia triggered by post-learning perturbations specifically impairs the labile memory. However, synaptic mechanisms for this selectivity remain elusive. Here, we show that diverse amnestic treatments, such as concussion, commonly disrupt the presynaptic clustering of Synapsin, which is required for anesthesia-sensitive memory. Consistently, targeted knockout of synaptojanin, a key regulator of the endocytic pathway, selectively impaired labile memory and the Synapsin-associated vesicles. In contrast, we identified Rab3, a small GTPase that regulates the late steps of vesicle exocytosis at the active zone, as selectively required for anesthesia-resistant memory. Rab3 hyperactivation enhanced its association with vesicles while displacing Synapsin. Strikingly, this manipulation biased memory toward stabilization at the cost of the labile component. We thus propose distinct vesicle pools at the presynaptic terminal underlie the formation of labile and consolidated memories. Thus, our work offers a molecular framework for controlling memory stability through targeted manipulation of vesicle dynamics.

  5. Chameau (HBO1) regulates starvation resistance in Drosophila melanogaster in a temperature-dependent manner. International-journal

    Anuroop Venkateswaran Venkatasubramani, Toshiharu Ichinose, Ignasi Forne, Nathaniel W Snyder, Hiromu Tanimoto, Shahaf Peleg, Axel Imhof

    Life science alliance　9　(1)　2026/01

    DOI： 10.26508/lsa.202503524 　

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    The body temperature of Drosophila melanogaster depends on the extrinsic temperature. Numerous studies show that environmental temperature influences metabolism, lifespan, and starvation resilience. We have previously shown that Chameau (Chm), a MYST-domain acetyltransferase, promotes aging but also increases starvation resilience. Strikingly, the metabolic increase associated with a 2°C temperature rise was sufficient to bypass the requirement for Chm in starvation resilience, suggesting that Chm modulates metabolism in a temperature-dependent manner. The increase in temperature also rescued the dampened expression of genes involved in starvation response, the weight loss, and the misregulation of trehalose, which we observed in chm mutants at 23°C. Thus, Chm regulates starvation at ≤23°C but becomes obsolete at higher temperatures, likely because of efficient acetyl-CoA generation ensuring similar acetylation despite lower Chm. Supporting this, citrate supplementation increased starvation resilience of chm mutants at lower temperatures. Our finding that a gene's role manifests only under specific environments has important implications in light of global climate change.

  6. Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neurons. International-journal

    Hongyang Wu, Sayaka Eno, Kyoko Jinnai, Ayako Abe, Kokoro Saito, Yoh Maekawa, Darren W Williams, Nobuhiro Yamagata, Shu Kondo, Hiromu Tanimoto

    PLoS biology　23　(10)　e3003449　2025/10/23

    DOI： 10.1371/journal.pbio.3003449 　

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    Neurons can adjust synaptic output according to the postsynaptic partners. However, the target-specific regulation of synaptic structures within individual neurons in the central nervous system remains unresolved. Applying the CRISPR/Cas9-mediated split-GFP tagging, we visualized the endogenous active zone scaffold protein, Bruchpilot (Brp), in specific cells. This technology enabled the spatial characterization of the presynaptic scaffolds only within the Kenyon cells (KCs) of the Drosophila mushroom bodies. We found the patterned accumulation of Brp among the compartments of axon terminals, where a KC synapses onto different postsynaptic neurons. Mechanistically, the localized octopaminergic projections along γ KC terminals regulate this compartmental Brp heterogeneity via Octβ2R and cAMP signaling. We further found that physiological stress, such as food or sleep deprivation reorganizes this intracellular pattern in an octopamine-dependent manner. Such concurrent regulation of local active zone assemblies thus suggests how the mushroom bodies integrate changing physiological states.

  7. Ecdysteroid-DopEcR signaling in neuronal and midgut cells mediates toxin avoidance and detoxification in Drosophila. International-journal

    Kokoro Saito, Mai Kanno, Hiromu Tanimoto, Toshiharu Ichinose

    Current biology : CB　35　(14)　3418-3428　2025/07/21

    DOI： 10.1016/j.cub.2025.06.023 　

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    Food is not only a source of nutrition but can also contain toxic substances. To survive, animals first need to avoid the ingestion of such harmful food, and if ingestion occurs, activate detoxification. However, the molecular coordination of these two functions has remained unclear. Using Drosophila melanogaster, we show that the major insect steroid hormone ecdysteroid and dopamine/ecdysteroid receptor (DopEcR) signaling play pivotal roles in both behavioral avoidance and detoxification of foods containing lethal levels of copper. Cell-type-specific knockdown experiments revealed that DopEcR signaling in neuronal cells mediates feeding aversion, while in the midgut copper cell region (CCR), it protects against copper toxicity. Upon copper ingestion, DopEcR in the CCR induces the expression of metallothionein (Mtn), a widely known detoxification protein. Furthermore, DopEcR mutants also failed to avoid and mitigate the effects of other hazardous substances such as paraquat and cocaine, suggesting a general role for DopEcR signaling in toxic food responses. This study demonstrates a cell-type-specific functional dissociation of ecdysteroid-DopEcR signaling for behavioral and physiological defenses against toxic foods.

  8. Gut microbiota-mediated lipid accumulation as a driver of evolutionary adaptation to blue light toxicity in Drosophila. International-journal

    Yuta Takada, Toshiharu Ichinose, Naoyuki Fuse, Kokoro Saito, Wakako Ikeda-Ohtsubo, Hiromu Tanimoto, Masatoshi Hori

    Communications biology　8　(1)　998-998　2025/07/07

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1038/s42003-025-08348-6 　

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    Blue light (BL), abundant in sunlight, is highly toxic to many insect species when exposure is excessive. While the physiological mechanisms of BL toxicity are being revealed, the evolutionary responses remain less explored. In this study, using Drosophila melanogaster as a model system, we conducted laboratory selection for over 60 generations under excessive BL exposure. The selected line (SL) flies exhibited enhanced BL-tolerance, and also increased body weight and lipid accumulation. Interestingly, we found elongated midgut in the SL flies, and increased microbiota, which was dominated by Acetobacter persici. The BL-tolerance and the lipid accumulation were dependent on the increment of gut microbiota. Genomic analysis identified mutations in the Hippo signaling pathway linked to midgut elongation, while transcriptome analysis showed downregulation of Tachykinin (Tk), a key suppressor of intestinal lipogenesis. Genetically induced lipid accumulation through manipulation of Tk or related lipogenic genes was sufficient to confer BL-tolerance. Moreover, our findings indicate that parental BL irradiation, along with accumulated mutations from laboratory selection, played a crucial role in midgut elongation and increased bacterial abundance. These findings reveal evolutionary responses to excessive BL exposure that shape host traits to maximize the benefits provided by gut microbiota.

  9. Satiety differentially modulates feeding steps in the jellyfish Cladonema. International-journal

    Genta Mashiba, Hiromu Tanimoto, Vladimiros Thoma

    iScience　28　(4)　112192-112192　2025/04/18

    DOI： 10.1016/j.isci.2025.112192 　

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    Following a meal, animals exhibit satiety, a state of decreased motivation to feed. Satiety is observed throughout the animal kingdom, suggesting ancient underlying mechanisms. Here, we investigate how satiety alters feeding in jellyfish, species that lack a centralized brain. Using comprehensive ethological analyses in Cladonema, a jellyfish with highly stereotyped, sequential feeding behavior, we show that satiety disorganizes its feeding sequence and delays all feeding steps, thus reducing food consumption. Surprisingly, isolated tentacles from fed jellyfish displayed satiety, thereby showing sustained and autonomous signaling of this state. Moreover, temporal dynamics of inhibition differed among feeding steps. Taken together, our results highlight complex satiety signaling in this species, suggesting multiple underlying signals.

  10. Synaptic enrichment and dynamic regulation of the two opposing dopamine receptors within the same neurons International-journal

    Shun Hiramatsu, Kokoro Saito, Shu Kondo, Hidetaka Katow, Nobuhiro Yamagata, Chun-Fang Wu, Hiromu Tanimoto

    eLife　13　2025/01/20

    Publisher: eLife Sciences Publications, Ltd

    DOI： 10.7554/elife.98358.2 　

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    Dopamine can play opposing physiological roles depending on the receptor subtype. In the fruit fly Drosophila melanogaster, Dop1R1 and Dop2R encode the D1- and D2-like receptors, respectively, and are reported to oppositely regulate intracellular cAMP levels. Here, we profiled the expression and subcellular localization of endogenous Dop1R1 and Dop2R in specific cell types in the mushroom body circuit. For cell-type-specific visualization of endogenous proteins, we employed reconstitution of split-GFP tagged to the receptor proteins. We detected dopamine receptors at both presynaptic and postsynaptic sites in multiple cell types. Quantitative analysis revealed enrichment of both receptors at the presynaptic sites, with Dop2R showing a greater degree of localization than Dop1R1. The presynaptic localization of Dop1R1 and Dop2R in dopamine neurons suggests dual feedback regulation as autoreceptors. Furthermore, we discovered a starvation-dependent, bidirectional modulation of the presynaptic receptor expression in the PAM and PPL1 clusters, two distinct subsets of dopamine neurons, suggesting regulation of appetitive behaviors. Our results highlight the significance of the co-expression of the two opposing dopamine receptors in the spatial and conditional regulation of dopamine responses in neurons.

  11. Profiling translation in the nervous system International-journal

    Toshiharu Ichinose, Hiromu Tanimoto

    The Journal of Biochemistry　177　(4)　239-246　2025/01/02

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/jb/mvae096 　

    ISSN： 0021-924X

    eISSN： 1756-2651

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    Abstract Regulation at the level of translation is critical for functions in the nervous system, such as the formation of cell-type specific proteomes or plastic changes in neural circuits. While current knowledge of the translatome is relatively limited compared to transcriptome, a growing array of tools to analyze translation is becoming available. In this review, we introduce techniques for profiling translation on a genome-wide scale with a special emphasis on cell-type specific analyses in the nervous system. This includes polysome-profiling-seq, Translating Ribosome Affinity Purification (TRAP)-seq and ribosome profiling (Ribo-seq). We review recent advances to achieve spatial resolution of translatome analysis, such as genetic labeling of the targeted cells and cell sorting, and discuss the biological implications of translational regulation in the brain and potential future extensions.

  12. Spatial Gradient of Axon Terminal Maturation Reveals the Sequential Active Zone Assembly in Adult Drosophila Mushroom Bodies. International-journal

    Hongyang Wu, William Constance, Ayako Abe, Shu Kondo, Darren Williams, Hiromu Tanimoto

    microPublication biology　2025　2025

    DOI： 10.17912/micropub.biology.001715 　

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    Presynaptic active zones (AZs) form during synaptogenesis and are critical for ensuring precise synaptic transmission. Although much is known about the development of AZs at the neuromuscular junction, their assembly in the Drosophila central nervous system (CNS) remains incompletely understood. Here, we demonstrate the mushroom body α/β Kenyon cells in young adults in which new AZs are continuously formed, as a novel system to dissect the sequential AZ assembly in the CNS. With this system we show proof of principle using a tagged allele of the cell-adhesion molecule Neurexin-1 to investigate the structural maturation of synapses in the fly brain.

  13. A high-protein diet-responsive gut hormone regulates behavioral and metabolic optimization in Drosophila melanogaster. International-journal

    Yuto Yoshinari, Takashi Nishimura, Taishi Yoshii, Shu Kondo, Hiromu Tanimoto, Tomoe Kobayashi, Makoto Matsuyama, Ryusuke Niwa

    Nature communications　15　(1)　10819-10819　2024/12/30

    DOI： 10.1038/s41467-024-55050-y 　

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    Protein is essential for all living organisms; however, excessive protein intake can have adverse effects, such as hyperammonemia. Although mechanisms responding to protein deficiency are well-studied, there is a significant gap in our understanding of how organisms adaptively suppress excessive protein intake. In the present study, utilizing the fruit fly, Drosophila melanogaster, we discover that the peptide hormone CCHamide1 (CCHa1), secreted by enteroendocrine cells in response to a high-protein diet (HPD), is vital for suppressing overconsumption of protein. Gut-derived CCHa1 is received by a small subset of enteric neurons that produce short neuropeptide F, thereby modulating protein-specific satiety. Importantly, impairment of the CCHa1-mediated gut-enteric neuronal axis results in ammonia accumulation and a shortened lifespan under HPD conditions. Collectively, our findings unravel the crosstalk of gut hormone and neuronal pathways that orchestrate physiological responses to prevent and adapt to dietary protein overload.

  14. Peer-induced quiescence of male Drosophila melanogaster following copulation

    Katrina Lynn, Toshiharu Ichinose, Hiromu Tanimoto

    Frontiers in Behavioral Neuroscience　18　2024/07/16

    Publisher: Frontiers Media SA

    DOI： 10.3389/fnbeh.2024.1414029 　

    eISSN： 1662-5153

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    Mating experience impacts the physiology and behavior of animals. Although mating effects of female Drosophila melanogaster have been studied extensively, the behavioral changes of males following copulation have not been fully understood. In this study, we characterized the mating-dependent behavioral changes of male flies, especially focusing on fly-to-fly interaction, and their dependence on rearing conditions. Our data demonstrate that male flies quiesce their courtship toward both females and males, as well as their locomotor activity. This post-copulatory quiescence appears to be contingent upon the presence of a peer, as minimal variation is noted in locomotion when the male is measured in isolation. Interestingly, copulated males influence a paired male without successful copulation to reduce his locomotion. Our findings point to a conditional behavioral quiescence following copulation, influenced by the presence of other flies.

  15. The fruit fly acetyltransferase chameau promotes starvation resilience at the expense of longevity. International-journal

    Anuroop Venkateswaran Venkatasubramani, Toshiharu Ichinose, Mai Kanno, Ignasi Forne, Hiromu Tanimoto, Shahaf Peleg, Axel Imhof

    EMBO reports　24　(10)　e57023　2023/10/09

    DOI： 10.15252/embr.202357023 　

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    Proteins involved in cellular metabolism and molecular regulation can extend lifespan of various organisms in the laboratory. However, any improvement in aging would only provide an evolutionary benefit if the organisms were able to survive under non-ideal conditions. We have previously shown that Drosophila melanogaster carrying a loss-of-function allele of the acetyltransferase chameau (chm) has an increased healthy lifespan when fed ad libitum. Here, we show that loss of chm and reduction in its activity results in a substantial reduction in weight and a decrease in starvation resistance. This phenotype is caused by failure to properly regulate the genes and proteins required for energy storage and expenditure. The previously observed increase in survival time thus comes with the inability to prepare for and cope with nutrient stress. As the ability to survive in environments with restricted food availability is likely a stronger evolutionary driver than the ability to live a long life, chm is still present in the organism's genome despite its apparent negative effect on lifespan.

  16. Translational regulation enhances distinction of cell types in the nervous system

    Toshiharu Ichinose, Shu Kondo, Mai Kanno, Yuichi Shichino, Mari Mito, Shintaro Iwasaki, Hiromu Tanimoto

    eLife　2023/09/26

    Publisher: eLife Sciences Publications, Ltd

    DOI： 10.7554/elife.90713 　

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    Multicellular organisms are composed of specialized cells with distinct proteomes. While recent advances in single-cell transcriptome analyses have revealed differential expression of mRNAs, cellular diversity in translational profiles remains to be understood. In this study, by performing RNA-seq and ribo-seq in genetically-defined cells in the Drosophila brain, we revealed substantial posttranscriptional regulations that augment the cell-type distinctions. Specifically, we found that translational efficiency of proteins fundamental to neuronal functions, such as ion channels and neurotransmitter receptors, was maintained low in glia, leading to preferential translation in neurons. Notably, distribution of ribosome footprints on these mRNAs exhibited a remarkable bias towards the 5′ untranslated regions (UTR) in glia. Using a transgenic reporter, we provide evidence that the UTR confer translational suppression selectively in glia. Overall, these findings underscore the profound impact of translational regulation in shaping cell identity and provide new insights into the molecular mechanisms driving cell-type diversity.

  17. A descending inhibitory mechanism of nociception mediated by an evolutionarily conserved neuropeptide system in Drosophila. International-journal

    Izumi Oikawa, Shu Kondo, Kao Hashimoto, Akiho Yoshida, Megumi Hamajima, Hiromu Tanimoto, Katsuo Furukubo-Tokunaga, Ken Honjo

    eLife　12　2023/06/13

    DOI： 10.7554/eLife.85760 　

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    Nociception is a neural process that animals have developed to avoid potentially tissue-damaging stimuli. While nociception is triggered in the peripheral nervous system, its modulation by the central nervous system is a critical process in mammals, whose dysfunction has been extensively implicated in chronic pain pathogenesis. The peripheral mechanisms of nociception are largely conserved across the animal kingdom. However, it is unclear whether the brain-mediated modulation is also conserved in non-mammalian species. Here, we show that Drosophila has a descending inhibitory mechanism of nociception from the brain, mediated by the neuropeptide Drosulfakinin (DSK), a homolog of cholecystokinin (CCK) that plays an important role in the descending control of nociception in mammals. We found that mutants lacking dsk or its receptors are hypersensitive to noxious heat. Through a combination of genetic, behavioral, histological, and Ca2+ imaging analyses, we subsequently revealed neurons involved in DSK-mediated nociceptive regulation at a single-cell resolution and identified a DSKergic descending neuronal pathway that inhibits nociception. This study provides the first evidence for a descending modulatory mechanism of nociception from the brain in a non-mammalian species that is mediated by the evolutionarily conserved CCK system, raising the possibility that the descending inhibition is an ancient mechanism to regulate nociception.

  18. Female reproductive dormancy in Drosophila is regulated by DH31-producing neurons projecting into the corpus allatum. International-journal

    Yoshitomo Kurogi, Eisuke Imura, Yosuke Mizuno, Ryo Hoshino, Marcela Nouzova, Shigeru Matsuyama, Akira Mizoguchi, Shu Kondo, Hiromu Tanimoto, Fernando G Noriega, Ryusuke Niwa

    Development (Cambridge, England)　150　(10)　2023/05/15

    DOI： 10.1242/dev.201186 　

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    Female insects can enter reproductive diapause, a state of suspended egg development, to conserve energy under adverse environments. In many insects, including the fruit fly, Drosophila melanogaster, reproductive diapause, also frequently called reproductive dormancy, is induced under low-temperature and short-day conditions by the downregulation of juvenile hormone (JH) biosynthesis in the corpus allatum (CA). In this study, we demonstrate that neuropeptide Diuretic hormone 31 (DH31) produced by brain neurons that project into the CA plays an essential role in regulating reproductive dormancy by suppressing JH biosynthesis in adult D. melanogaster. The CA expresses the gene encoding the DH31 receptor, which is required for DH31-triggered elevation of intracellular cAMP in the CA. Knocking down Dh31 in these CA-projecting neurons or DH31 receptor in the CA suppresses the decrease of JH titer, normally observed under dormancy-inducing conditions, leading to abnormal yolk accumulation in the ovaries. Our findings provide the first molecular genetic evidence demonstrating that CA-projecting peptidergic neurons play an essential role in regulating reproductive dormancy by suppressing JH biosynthesis.

  19. On the origin of appetite: GLWamide in jellyfish represents an ancestral satiety neuropeptide. International-journal

    Vladimiros Thoma, Shuhei Sakai, Koki Nagata, Yuu Ishii, Shinichiro Maruyama, Ayako Abe, Shu Kondo, Masakado Kawata, Shun Hamada, Ryusaku Deguchi, Hiromu Tanimoto

    Proceedings of the National Academy of Sciences of the United States of America　120　(15)　e2221493120　2023/04/11

    DOI： 10.1073/pnas.2221493120 　

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    Food intake is regulated by internal state. This function is mediated by hormones and neuropeptides, which are best characterized in popular model species. However, the evolutionary origins of such feeding-regulating neuropeptides are poorly understood. We used the jellyfish Cladonema to address this question. Our combined transcriptomic, behavioral, and anatomical approaches identified GLWamide as a feeding-suppressing peptide that selectively inhibits tentacle contraction in this jellyfish. In the fruit fly Drosophila, myoinhibitory peptide (MIP) is a related satiety peptide. Surprisingly, we found that GLWamide and MIP were fully interchangeable in these evolutionarily distant species for feeding suppression. Our results suggest that the satiety signaling systems of diverse animals share an ancient origin.

  20. Functional impact of subunit composition and compensation on Drosophila melanogaster nicotinic receptors-targets of neonicotinoids. International-journal

    Yuma Komori, Koichi Takayama, Naoki Okamoto, Masaki Kamiya, Wataru Koizumi, Makoto Ihara, Daitaro Misawa, Kotaro Kamiya, Yuto Yoshinari, Kazuki Seike, Shu Kondo, Hiromu Tanimoto, Ryusuke Niwa, David B Sattelle, Kazuhiko Matsuda

    PLoS genetics　19　(2)　e1010522　2023/02

    DOI： 10.1371/journal.pgen.1010522 　

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    Neonicotinoid insecticides target insect nicotinic acetylcholine receptors (nAChRs) and their adverse effects on non-target insects are of serious concern. We recently found that cofactor TMX3 enables robust functional expression of insect nAChRs in Xenopus laevis oocytes and showed that neonicotinoids (imidacloprid, thiacloprid, and clothianidin) exhibited agonist actions on some nAChRs of the fruit fly (Drosophila melanogaster), honeybee (Apis mellifera) and bumblebee (Bombus terrestris) with more potent actions on the pollinator nAChRs. However, other subunits from the nAChR family remain to be explored. We show that the Dα3 subunit co-exists with Dα1, Dα2, Dβ1, and Dβ2 subunits in the same neurons of adult D. melanogaster, thereby expanding the possible nAChR subtypes in these cells alone from 4 to 12. The presence of Dα1 and Dα2 subunits reduced the affinity of imidacloprid, thiacloprid, and clothianidin for nAChRs expressed in Xenopus laevis oocytes, whereas the Dα3 subunit enhanced it. RNAi targeting Dα1, Dα2 or Dα3 in adults reduced expression of targeted subunits but commonly enhanced Dβ3 expression. Also, Dα1 RNAi enhanced Dα7 expression, Dα2 RNAi reduced Dα1, Dα6, and Dα7 expression and Dα3 RNAi reduced Dα1 expression while enhancing Dα2 expression, respectively. In most cases, RNAi treatment of either Dα1 or Dα2 reduced neonicotinoid toxicity in larvae, but Dα2 RNAi enhanced neonicotinoid sensitivity in adults reflecting the affinity-reducing effect of Dα2. Substituting each of Dα1, Dα2, and Dα3 subunits by Dα4 or Dβ3 subunit mostly increased neonicotinoid affinity and reduced efficacy. These results are important because they indicate that neonicotinoid actions involve the integrated activity of multiple nAChR subunit combinations and counsel caution in interpreting neonicotinoid actions simply in terms of toxicity.

  21. Neural control of redox response and microbiota-triggered inflammation in Drosophila gut. International-journal

    Naoyuki Fuse, Haruka Hashiba, Kentaro Ishibashi, Takuro Suzuki, Quang-Dat Nguyen, Kiho Fujii, Wakako Ikeda-Ohtsubo, Haruki Kitazawa, Hiromu Tanimoto, Shoichiro Kurata

    Frontiers in immunology　14　1268611-1268611　2023

    DOI： 10.3389/fimmu.2023.1268611 　

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    BACKGROUND: The neural system plays a critical role in controlling gut immunity, and the gut microbiota contributes to this process. However, the roles and mechanisms of gut-brain-microbiota interactions remain unclear. To address this issue, we employed Drosophila as a model organism. We have previously shown that NP3253 neurons, which are connected to the brain and gut, are essential for resistance to oral bacterial infections. Here, we aimed to investigate the role of NP3253 neurons in the regulation of gut immunity. METHODS: We performed RNA-seq analysis of the adult Drosophila gut after genetically inactivating the NP3253 neurons. Flies were reared under oral bacterial infection and normal feeding conditions. In addition, we prepared samples under germ-free conditions to evaluate the role of the microbiota in gut gene expression. We knocked down the genes regulated by NP3253 neurons and examined their susceptibility to oral bacterial infections. RESULTS: We found that immune-related gene expression was upregulated in NP3253 neuron-inactivated flies compared to the control. However, this upregulation was abolished in axenic flies, suggesting that the immune response was abnormally activated by the microbiota in NP3253 neuron-inactivated flies. In addition, redox-related gene expression was downregulated in NP3253 neuron-inactivated flies, and this downregulation was also observed in axenic flies. Certain redox-related genes were required for resistance to oral bacterial infections, suggesting that NP3253 neurons regulate the redox responses for gut immunity in a microbiota-independent manner. CONCLUSION: These results show that NP3253 neurons regulate the appropriate gene expression patterns in the gut and contribute to maintain homeostasis during oral infections.

  22. Nutrient responding peptide hormone CCHamide-2 consolidates appetitive memory. International-journal

    Nobuhiro Yamagata, Yasuhito Imanishi, Hongyang Wu, Shu Kondo, Hiroko Sano, Hiromu Tanimoto

    Frontiers in behavioral neuroscience　16　986064-986064　2022

    DOI： 10.3389/fnbeh.2022.986064 　

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    CCHamide-2 (CCHa2) is a protostome excitatory peptide ortholog known for various arthropod species. In fruit flies, CCHa2 plays a crucial role in the endocrine system, allowing peripheral tissue to communicate with the central nervous system to ensure proper development and the maintenance of energy homeostasis. Since the formation of odor-sugar associative long-term memory (LTM) depends on the nutrient status in an animal, CCHa2 may play an essential role in linking memory and metabolic systems. Here we show that CCHa2 signals are important for consolidating appetitive memory by acting on the rewarding dopamine neurons. Genetic disruption of CCHa2 using mutant strains abolished appetitive LTM but not short-term memory (STM). A post-learning thermal suppression of CCHa2 expressing cells impaired LTM. In contrast, a post-learning thermal activation of CCHa2 cells stabilized STM induced by non-nutritious sugar into LTM. The receptor of CCHa2, CCHa2-R, was expressed in a subset of dopamine neurons that mediate reward for LTM. In accordance, the receptor expression in these dopamine neurons was required for LTM specifically. We thus concluded that CCHa2 conveys a sugar nutrient signal to the dopamine neurons for memory consolidation. Our finding establishes a direct interplay between brain reward and the putative endocrine system for long-term energy homeostasis.

  23. The sugar-responsive enteroendocrine neuropeptide F regulates lipid metabolism through glucagon-like and insulin-like hormones in Drosophila melanogaster International-journal

    Yuto Yoshinari, Hina Kosakamoto, Takumi Kamiyama, Ryo Hoshino, Rena Matsuoka, Shu Kondo, Hiromu Tanimoto, Akira Nakamura, Fumiaki Obata, Ryusuke Niwa

    Nature communications　12　(1)　4818-4818　2021/08/10

    DOI： 10.1038/s41467-021-25146-w 　

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    The enteroendocrine cell (EEC)-derived incretins play a pivotal role in regulating the secretion of glucagon and insulins in mammals. Although glucagon-like and insulin-like hormones have been found across animal phyla, incretin-like EEC-derived hormones have not yet been characterised in invertebrates. Here, we show that the midgut-derived hormone, neuropeptide F (NPF), acts as the sugar-responsive, incretin-like hormone in the fruit fly, Drosophila melanogaster. Secreted NPF is received by NPF receptor in the corpora cardiaca and in insulin-producing cells. NPF-NPFR signalling resulted in the suppression of the glucagon-like hormone production and the enhancement of the insulin-like peptide secretion, eventually promoting lipid anabolism. Similar to the loss of incretin function in mammals, loss of midgut NPF led to significant metabolic dysfunction, accompanied by lipodystrophy, hyperphagia, and hypoglycaemia. These results suggest that enteroendocrine hormones regulate sugar-dependent metabolism through glucagon-like and insulin-like hormones not only in mammals but also in insects.

  24. Presynaptic inhibition of dopamine neurons controls optimistic bias International-journal Peer-reviewed

    Nobuhiro Yamagata, Takahiro Ezaki, Takahiro Takahashi, Hongyang Wu, Hiromu Tanimoto

    eLife　10　2021/06/01

    DOI： 10.7554/eLife.64907 　

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    Regulation of reward signaling in the brain is critical for appropriate judgement of the environment and self. In Drosophila, the protocerebral anterior medial (PAM) cluster dopamine neurons mediate reward signals. Here, we show that localized inhibitory input to the presynaptic terminals of the PAM neurons titrates olfactory reward memory and controls memory specificity. The inhibitory regulation was mediated by metabotropic gamma-aminobutyric acid (GABA) receptors clustered in presynaptic microdomain of the PAM boutons. Cell type-specific silencing the GABA receptors enhanced memory by augmenting internal reward signals. Strikingly, the disruption of GABA signaling reduced memory specificity to the rewarded odor by changing local odor representations in the presynaptic terminals of the PAM neurons. The inhibitory microcircuit of the dopamine neurons is thus crucial for both reward values and memory specificity. Maladaptive presynaptic regulation causes optimistic cognitive bias.

  25. A population of neurons that produce hugin and express the diuretic hormone 44 receptor gene projects to the corpora allata in Drosophila melanogaster Peer-reviewed

    Yosuke Mizuno, Eisuke Imura, Yoshitomo Kurogi, Yuko Shimada-Niwa, Shu Kondo, Hiromu Tanimoto, Sebastian Hückesfeld, Michael J Pankratz, Ryusuke Niwa

    Development, growth & differentiation　63　(4-5)　249-261　2021/05

    DOI： 10.1111/dgd.12733 　

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    The corpora allata (CA) are essential endocrine organs that biosynthesize and secrete the sesquiterpenoid hormone, namely juvenile hormone (JH), to regulate a wide variety of developmental and physiological events in insects. CA are directly innervated with neurons in many insect species, implying the innervations to be important for regulating JH biosynthesis. Although this is also true for the model organism Drosophila melanogaster, neurotransmitters produced in the CA-projecting neurons are yet to be identified. In this study on D. melanogaster, we aimed to demonstrate that a subset of neurons producing the neuropeptide hugin, the invertebrate counterpart of the vertebrate neuromedin U, directly projects to the adult CA. A synaptic vesicle marker in the hugin neurons was observed at their axon termini located on the CA, which were immunolabeled with a newly-generated antibody to the JH biosynthesis enzyme JH acid O-methyltransferase. We also found the CA-projecting hugin neurons to likely express a gene encoding the specific receptor for diuretic hormone 44 (Dh44). Moreover, our data suggest that the CA-projecting hugin neurons have synaptic connections with the upstream neurons producing Dh44. Unexpectedly, the inhibition of CA-projecting hugin neurons did not significantly alter the expression levels of the JH-inducible gene Krüppel-homolog 1, which implies that the CA-projecting neurons are not involved in JH biosynthesis but rather in other known biological processes. This is the first study to identify a specific neurotransmitter of the CA-projecting neurons in D. melanogaster, and to anatomically characterize a neuronal pathway of the CA-projecting neurons and their upstream neurons.

  26. Drosophila acquires seconds-scale rhythmic behavior International-journal Peer-reviewed

    Masayoshi Ikarashi, Hiromu Tanimoto

    The Journal of experimental biology　2021/04/01

    DOI： 10.1242/jeb.242443 　

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    Detection of the temporal structure of stimuli is crucial for prediction. While perception of interval timing is relevant for immediate behavioral adaptations, it has been scarcely investigated, especially in invertebrates. Here we examined if the fruit fly, Drosophila melanogaster, can acquire rhythmic behavior in the range of seconds. To this end, we developed a novel temporal conditioning paradigm utilizing repeated electric shocks. Combined automatic behavioral annotation and time-frequency analysis revealed that behavioral rhythms continued after cessation of the shocks. Furthermore, we found that aging impaired interval timing. This study thus not only demonstrated the ability of insects to acquire behavioral rhythms of a few seconds, but highlighted a life-course decline of temporal coordination, that is common also in mammals.

  27. Mushroom body output differentiates memory processes and distinct memory-guided behaviors Peer-reviewed

    Toshiharu Ichinose, Mai Kanno, Hongyang Wu, Nobuhiro Yamagata, Huan Sun, Ayako Abe, Hiromu Tanimoto

    Current Biology　31　(6)　1294-1302.e4　2021/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.cub.2020.12.032 　

    ISSN： 0960-9822

  28. Voluntary intake of psychoactive substances is regulated by the dopamine receptor Dop1R1 in Drosophila International-journal Peer-reviewed

    Mai Kanno, Shun Hiramatsu, Shu Kondo, Hiromu Tanimoto, Toshiharu Ichinose

    Scientific reports　11　(1)　3432-3432　2021/02/09

    DOI： 10.1038/s41598-021-82813-0 　

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    Dysregulated motivation to consume psychoactive substances leads to addictive behaviors that often result in serious health consequences. Understanding the neuronal mechanisms that drive drug consumption is crucial for developing new therapeutic strategies. The fruit fly Drosophila melanogaster offers a unique opportunity to approach this problem with a battery of sophisticated neurogenetic tools available, but how they consume these drugs remains largely unknown. Here, we examined drug self-administration behavior of Drosophila and the underlying neuronal mechanisms. We measured the preference of flies for five different psychoactive substances using a two-choice feeding assay and monitored its long-term changes. We found that flies show acute preference for ethanol and methamphetamine, but not for cocaine, caffeine or morphine. Repeated intake of ethanol, but not methamphetamine, increased over time. Preference for methamphetamine and the long-term escalation of ethanol preference required the dopamine receptor Dop1R1 in the mushroom body. The protein level of Dop1R1 increased after repeated intake of ethanol, but not methamphetamine, which correlates with the acquired preference. Genetic overexpression of Dop1R1 enhanced ethanol preference. These results reveal a striking diversity of response to individual drugs in the fly and the role of dopamine signaling and its plastic changes in controlling voluntary intake of drugs.

  29. Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster International-journal Peer-reviewed

    Yuto Yoshinari, Tomotsune Ameku, Shu Kondo, Hiromu Tanimoto, Takayuki Kuraishi, Yuko Shimada-Niwa, Ryusuke Niwa

    eLife　9　2020/10/20

    DOI： 10.7554/eLife.57101 　

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    Stem cells fuel the development and maintenance of tissues. Many studies have addressed how local signals from neighboring niche cells regulate stem cell identity and their proliferative potential. However, the regulation of stem cells by tissue-extrinsic signals in response to environmental cues remains poorly understood. Here we report that efferent octopaminergic neurons projecting to the ovary are essential for germline stem cell (GSC) increase in response to mating in female Drosophila. The neuronal activity of the octopaminergic neurons is required for mating-induced GSC increase as they relay the mating signal from sex peptide receptor-positive cholinergic neurons. Octopamine and its receptor Oamb are also required for mating-induced GSC increase via intracellular Ca2+ signaling. Moreover, we identified Matrix metalloproteinase-2 as a downstream component of the octopamine-Ca2+ signaling to induce GSC increase. Our study provides a mechanism describing how neuronal system couples stem cell behavior to environmental cues through stem cell niche signaling.

  30. Cofactor-enabled functional expression of fruit fly, honeybee, and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions International-journal Peer-reviewed

    Makoto Ihara, Shogo Furutani, Sho Shigetou, Shota Shimada, Kunihiro Niki, Yuma Komori, Masaki Kamiya, Wataru Koizumi, Leo Magara, Mai Hikida, Akira Noguchi, Daiki Okuhara, Yuto Yoshinari, Shu Kondo, Hiromu Tanimoto, Ryusuke Niwa, David B Sattelle, Kazuhiko Matsuda

    Proceedings of the National Academy of Sciences of the United States of America　117　(28)　16283-16291　2020/07/14

    DOI： 10.1073/pnas.2003667117 　

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    The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollinators. We show that thioredoxin-related transmembrane protein 3 (TMX3) is essential to enable robust expression in Xenopus laevis oocytes of honeybee (Apis mellifera) and bumblebee (Bombus terrestris) as well as fruit fly (Drosophila melanogaster) nAChR heteromers targeted by neonicotinoids and not hitherto robustly expressed. This has enabled the characterization of picomolar target site actions of neonicotinoids, findings important in understanding their toxicity.

  31. The Corazonin-PTTH Neuronal Axis Controls Systemic Body Growth by Regulating Basal Ecdysteroid Biosynthesis in Drosophila melanogaster International-journal Peer-reviewed

    Eisuke Imura, Yuko Shimada-Niwa, Takashi Nishimura, Sebastian Hückesfeld, Philipp Schlegel, Yuya Ohhara, Shu Kondo, Hiromu Tanimoto, Albert Cardona, Michael J Pankratz, Ryusuke Niwa

    Current biology : CB　30　(11)　2156-2165　2020/06/08

    DOI： 10.1016/j.cub.2020.03.050 　

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    Steroid hormones play key roles in development, growth, and reproduction in various animal phyla [1]. The insect steroid hormone, ecdysteroid, coordinates growth and maturation, represented by molting and metamorphosis [2]. In Drosophila melanogaster, the prothoracicotropic hormone (PTTH)-producing neurons stimulate peak levels of ecdysteroid biosynthesis for maturation [3]. Additionally, recent studies on PTTH signaling indicated that basal levels of ecdysteroid negatively affect systemic growth prior to maturation [4-8]. However, it remains unclear how PTTH signaling is regulated for basal ecdysteroid biosynthesis. Here, we report that Corazonin (Crz)-producing neurons regulate basal ecdysteroid biosynthesis by affecting PTTH neurons. Crz belongs to gonadotropin-releasing hormone (GnRH) superfamily, implying an analogous role in growth and maturation [9]. Inhibition of Crz neuronal activity increased pupal size, whereas it hardly affected pupariation timing. This phenotype resulted from enhanced growth rate and a delay in ecdysteroid elevation during the mid-third instar larval (L3) stage. Interestingly, Crz receptor (CrzR) expression in PTTH neurons was higher during the mid- than the late-L3 stage. Silencing of CrzR in PTTH neurons increased pupal size, phenocopying the inhibition of Crz neuronal activity. When Crz neurons were optogenetically activated, a strong calcium response was observed in PTTH neurons during the mid-L3, but not the late-L3, stage. Furthermore, we found that octopamine neurons contact Crz neurons in the subesophageal zone (SEZ), transmitting signals for systemic growth. Together, our results suggest that the Crz-PTTH neuronal axis modulates ecdysteroid biosynthesis in response to octopamine, uncovering a regulatory neuroendocrine system in the developmental transition from growth to maturation.

  32. Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila International-journal Peer-reviewed

    Huan Sun, Tomoki Nishioka, Shun Hiramatsu, Shu Kondo, Mutsuki Amano, Kozo Kaibuchi, Toshiharu Ichinose, Hiromu Tanimoto

    The Journal of neuroscience : the official journal of the Society for Neuroscience　40　(14)　2935-2942　2020/04/01

    DOI： 10.1523/JNEUROSCI.1572-19.2020 　

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    In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here we address this question in the context of sugar-rewarded olfactory long-term memory (LTM). We show that associative training increases the phosphorylation of MAPK in KCs, via Dop1R2 signaling. Consistently, the attenuation of Dop1R2, Raf, or MAPK expression in KCs selectively impairs LTM, but not short-term memory. Moreover, we show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.SIGNIFICANCE STATEMENT Dopaminergic input to the Kenyon cells (KCs) is pivotal to stabilize memory in Drosophila This process is mediated by dopamine receptors like Dop1R2. Nevertheless, little is known for its underlying molecular mechanism. Here we show that the Raf/MAPK pathway is specifically engaged in appetitive long-term memory in KCs. With combined biochemical and behavioral experiments, we reveal that activation of the Raf/MAPK pathway is regulated through Dop1R2, shedding light on how dopamine modulates intracellular signaling for memory stabilization.

  33. Future perspectives of neurogenetics - in honor of Troy D. Zars (1967-2018) International-journal Peer-reviewed

    Bertram Gerber, Elizabeth G King, David Schulz, Hiromu Tanimoto, Scott Waddell, Chun-Fang Wu

    Journal of neurogenetics　34　(1)　1-1　2020/03

    DOI： 10.1080/01677063.2020.1715975 　

  34. Environmental Light Is Required for Maintenance of Long-Term Memory in Drosophila International-journal Peer-reviewed

    Show Inami, Shoma Sato, Shu Kondo, Hiromu Tanimoto, Toshihiro Kitamoto, Takaomi Sakai

    The Journal of neuroscience : the official journal of the Society for Neuroscience　40　(7)　1427-1439　2020/02/12

    DOI： 10.1523/JNEUROSCI.1282-19.2019 　

    ISSN： 0270-6474

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    Long-term memory (LTM) is stored as functional modifications of relevant neural circuits in the brain. A large body of evidence indicates that the initial establishment of such modifications through the process known as memory consolidation requires learning-dependent transcriptional activation and de novo protein synthesis. However, it remains poorly understood how the consolidated memory is maintained for a long period in the brain, despite constant turnover of molecular substrates. Using the Drosophila courtship conditioning assay of adult males as a memory paradigm, here, we show that in Drosophila, environmental light plays a critical role in LTM maintenance. LTM is impaired when flies are kept in constant darkness (DD) during the memory maintenance phase. Because light activates the brain neurons expressing the neuropeptide pigment-dispersing factor (Pdf), we examined the possible involvement of Pdf neurons in LTM maintenance. Temporal activation of Pdf neurons compensated for the DD-dependent LTM impairment, whereas temporal knockdown of Pdf during the memory maintenance phase impaired LTM in light/dark cycles. Furthermore, we demonstrated that the transcription factor cAMP response element-binding protein (CREB) is required in the memory center, namely, the mushroom bodies (MBs), for LTM maintenance, and Pdf signaling regulates light-dependent transcription via CREB. Our results demonstrate for the first time that universally available environmental light plays a critical role in LTM maintenance by activating the evolutionarily conserved memory modulator CREB in MBs via the Pdf signaling pathway.SIGNIFICANCE STATEMENT Temporary memory can be consolidated into long-term memory (LTM) through de novo protein synthesis and functional modifications of neuronal circuits in the brain. Once established, LTM requires continual maintenance so that it is kept for an extended period against molecular turnover and cellular reorganization that may disrupt memory traces. How is LTM maintained mechanistically? Despite the critical importance of LTM maintenance, its molecular and cellular underpinnings remain elusive. This study using Drosophila is significant because it revealed for the first time in any organism that universally available environmental light plays an essential role in LTM maintenance. Interestingly, light does so by activating the evolutionarily conserved transcription factor cAMP response element-binding protein via peptidergic signaling.

  35. Neurochemical Organization of the Drosophila Brain Visualized by Endogenously Tagged Neurotransmitter Receptors International-journal Peer-reviewed

    Shu Kondo, Takahiro Takahashi, Nobuhiro Yamagata, Yasuhito Imanishi, Hidetaka Katow, Shun Hiramatsu, Katrina Lynn, Ayako Abe, Ajayrama Kumaraswamy, Hiromu Tanimoto

    Cell reports　30　(1)　284-297　2020/01/07

    DOI： 10.1016/j.celrep.2019.12.018 　

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    Neurotransmitters often have multiple receptors that induce distinct responses in receiving cells. Expression and localization of neurotransmitter receptors in individual neurons are therefore critical for understanding the operation of neural circuits. Here we describe a comprehensive library of reporter strains in which a convertible T2A-GAL4 cassette is inserted into endogenous neurotransmitter receptor genes of Drosophila. Using this library, we profile the expression of 75 neurotransmitter receptors in the brain. Cluster analysis reveals neurochemical segmentation of the brain, distinguishing higher brain centers from the rest. By recombinase-mediated cassette exchange, we convert T2A-GAL4 into split-GFP and Tango to visualize subcellular localization and activation of dopamine receptors in specific cell types. This reveals striking differences in their subcellular localization, which may underlie the distinct cellular responses to dopamine in different behavioral contexts. Our resources thus provide a versatile toolkit for dissecting the cellular organization and function of neurotransmitter systems in the fly brain.

  36. Bodily Awareness: How Flies Learn Their Own Body Size International-journal Peer-reviewed

    Hiromu Tanimoto

    Current biology : CB　29　(12)　R572-R574-R574　2019/06/17

    DOI： 10.1016/j.cub.2019.05.007 　

    ISSN： 0960-9822

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    Animals need to perceive their own body size to apprehend their relationship to the environment. A new study shows that the fruit fly Drosophila acquires the requisite information on its body size from visual feedback during walking, and has further identified a subset of neurons responsible for maintenance of body-size memory.

  37. Courtship behavior induced by appetitive olfactory memory Peer-reviewed

    Yuya Onodera, Rino Ichikawa, Kanta Terao, Hiromu Tanimoto, Nobuhiro Yamagata

    Journal of neurogenetics　33　(2)　1-9　2019/04

    DOI： 10.1080/01677063.2019.1593978 　

    ISSN： 0167-7063

  38. Tango knock-ins visualize endogenous activity of G protein-coupled receptors in Drosophila Peer-reviewed

    Hidetaka Katow, Takahiro Takahashi, Kuniaki Saito, Hiromu Tanimoto, Shu Kondo

    Journal of neurogenetics　33　(2)　44-51　2019/03

    DOI： 10.1080/01677063.2019.1611806 　

    ISSN： 0167-7063

  39. Comparative behavioral genetics: the Yamamoto approach Peer-reviewed

    Hiromu Tanimoto, Chun-Fang Wu

    Journal of neurogenetics　33　(2)　41-43　2019/03

    DOI： 10.1080/01677063.2019.1616720 　

    ISSN： 0167-7063

  40. Quantification of Aggregation and Associated Brain Areas in Drosophila Melanogaster. Peer-reviewed

    Takuto Okuno, Koichi Hashimoto, Hiromu Tanimoto

    IEEE International Conference on Pervasive Computing and Communications Workshops, PerCom Workshops 2019, Kyoto, Japan, March 11-15, 2019　759-764　2019

    Publisher: IEEE

    DOI： 10.1109/PERCOMW.2019.8730594 　

  41. Data-driven analysis of motor activity implicates 5-HT2A neurons in backward locomotion of larval Drosophila International-journal Peer-reviewed

    Jeonghyuk Park, Shu Kondo, Hiromu Tanimoto, Hiroshi Kohsaka, Akinao Nose

    Scientific reports　8　(1)　10307-10307　2018/07/09

    DOI： 10.1038/s41598-018-28680-8 　

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    Rhythmic animal behaviors are regulated in part by neural circuits called the central pattern generators (CPGs). Classifying neural population activities correlated with body movements and identifying the associated component neurons are critical steps in understanding CPGs. Previous methods that classify neural dynamics obtained by dimension reduction algorithms often require manual optimization which could be laborious and preparation-specific. Here, we present a simpler and more flexible method that is based on the pre-trained convolutional neural network model VGG-16 and unsupervised learning, and successfully classifies the fictive motor patterns in Drosophila larvae under various imaging conditions. We also used voxel-wise correlation mapping to identify neurons associated with motor patterns. By applying these methods to neurons targeted by 5-HT2A-GAL4, which we generated by the CRISPR/Cas9-system, we identified two classes of interneurons, termed Seta and Leta, which are specifically active during backward but not forward fictive locomotion. Optogenetic activation of Seta and Leta neurons increased backward locomotion. Conversely, thermogenetic inhibition of 5-HT2A-GAL4 neurons or application of a 5-HT2 antagonist decreased backward locomotion induced by noxious light stimuli. This study establishes an accelerated pipeline for activity profiling and cell identification in larval Drosophila and implicates the serotonergic system in the modulation of backward locomotion.

  42. The role of the gustatory system in the coordination of feeding Peer-reviewed

    Vladimiros Thoma, Kimiko Kobayashi, Hiromu Tanimoto

    eNeuro　4　(6)　2017/11/01

    Publisher: Society for Neuroscience

    DOI： 10.1523/ENEURO.0324-17.2017 　

    ISSN： 2373-2822

  43. Behavioral Modulation by Spontaneous Activity of Dopamine Neurons International-journal Peer-reviewed

    Toshiharu Ichinose, Hiromu Tanimoto, Nobuhiro Yamagata

    Frontiers in systems neuroscience　11　88-88　2017

    DOI： 10.3389/fnsys.2017.00088 　

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    Dopamine modulates a variety of animal behaviors that range from sleep and learning to courtship and aggression. Besides its well-known phasic firing to natural reward, a substantial number of dopamine neurons (DANs) are known to exhibit ongoing intrinsic activity in the absence of an external stimulus. While accumulating evidence points at functional implications for these intrinsic "spontaneous activities" of DANs in cognitive processes, a causal link to behavior and its underlying mechanisms has yet to be elucidated. Recent physiological studies in the model organism Drosophila melanogaster have uncovered that DANs in the fly brain are also spontaneously active, and that this activity reflects the behavioral/internal states of the animal. Strikingly, genetic manipulation of basal DAN activity resulted in behavioral alterations in the fly, providing critical evidence that links spontaneous DAN activity to behavioral states. Furthermore, circuit-level analyses have started to reveal cellular and molecular mechanisms that mediate or regulate spontaneous DAN activity. Through reviewing recent findings in different animals with the major focus on flies, we will discuss potential roles of this physiological phenomenon in directing animal behaviors.

  44. Suppression of Dopamine Neurons Mediates Reward International-journal Peer-reviewed

    Nobuhiro Yamagata, Makoto Hiroi, Shu Kondo, Ayako Abe, Hiromu Tanimoto

    PLoS biology　14　(12)　e1002586-e1002586　2016/12

    DOI： 10.1371/journal.pbio.1002586 　

    ISSN： 1544-9173

  45. Functional dissociation in sweet taste receptor neurons between and within taste organs of Drosophila Peer-reviewed

    Vladimiros Thoma, Stephan Knapek, Shogo Arai, Marion Hartl, Hiroshi Kohsaka, Pudith Sirigrivatanawong, Ayako Abe, Koichi Hashimoto, Hiromu Tanimoto

    CHEMICAL SENSES　41　(9)　E171-E171　2016/11

    ISSN： 0379-864X

    eISSN： 1464-3553

  46. Dynamics of memory-guided choice behavior in Drosophila Peer-reviewed

    Toshiharu Ichinose, Hiromu Tanimoto

    PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES　92　(8)　346-357　2016/10

    DOI： 10.2183/pjab.92.346 　

    ISSN： 0386-2208

  47. Direct neural pathways convey distinct visual information to Drosophila mushroom bodies Peer-reviewed

    Katrin Vogt, Yoshinori Aso, Toshihide Hige, Stephan Knapek, Toshiharu Ichinose, Anja B. Friedrich, Glenn C. Turner, Gerald M. Rubin, Hiromu Tanimoto

    ELIFE　5　2016/04

    DOI： 10.7554/eLife.14009 　

    ISSN： 2050-084X

  48. Four Individually Identified Paired Dopamine Neurons Signal Reward in Larval Drosophila Peer-reviewed

    Astrid Rohwedder, Nana L. Wenz, Bernhard Stehle, Annina Huser, Nobuhiro Yamagata, Marta Zlatic, James W. Truman, Hiromu Tanimoto, Timo Saumweber, Bertram Gerber, Andreas S. Thum

    CURRENT BIOLOGY　26　(5)　661-669　2016/03

    DOI： 10.1016/j.cub.2016.01.012 　

    ISSN： 0960-9822

    eISSN： 1879-0445

  49. Functional dissociation in sweet taste receptor neurons between and within taste organs of Drosophila Peer-reviewed

    Vladimiros Thoma, Stephan Knapek, Shogo Arai, Marion Hartl, Hiroshi Kohsaka, Pudith Sirigrivatanawong, Ayako Abe, Koichi Hashimoto, Hiromu Tanimoto

    NATURE COMMUNICATIONS　7　10678-10678　2016/02

    DOI： 10.1038/ncomms10678 　

    ISSN： 2041-1723

  50. Suppression of dopamine neurons for memory formation Peer-reviewed

    Nobuhiro Yamagata, Makoto Hiroi, Shu Kondo, Ayako Abe, Tetsuya Tabata, Hiromu Tanimoto

    GENES & GENETIC SYSTEMS　90　(6)　366-366　2015/12

    ISSN： 1341-7568

    eISSN： 1880-5779

  51. Reward signal in a recurrent circuit drives appetitive long-term memory formation Peer-reviewed

    Toshiharu Ichinose, Yoshinori Aso, Nobuhiro Yamagata, Ayako Abe, Gerald M. Rubin, Hiromu Tanimoto

    ELIFE　4　e10719-e10719　2015/11

    DOI： 10.7554/eLife.10719 　

    ISSN： 2050-084X

  52. Reversing Stimulus Timing in Visual Conditioning Leads to Memories with Opposite Valence in Drosophila Peer-reviewed

    Katrin Vogt, Ayse Yarali, Hiromu Tanimoto

    PLOS ONE　10　(10)　e0139797-e0139797　2015/10

    DOI： 10.1371/journal.pone.0139797 　

    ISSN： 1932-6203

  53. Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster Peer-reviewed

    Mirjam Appel, Claus-Juergen Scholz, Tobias Mueller, Marcus Dittrich, Christian Koenig, Marie Bockstaller, Tuba Oguz, Afshin Khalili, Emmanuel Antwi-Adjei, Tamas Schauer, Carla Margulies, Hiromu Tanimoto, Ayse Yarali

    PLOS ONE　10　(5)　e0126986-e0126986　2015/05

    DOI： 10.1371/journal.pone.0126986 　

    ISSN： 1932-6203

  54. A model for non-monotonic intensity coding Peer-reviewed

    Johannes Nehrkorn, Hiromu Tanimoto, Andreas V. M. Herz, Ayse Yarali

    ROYAL SOCIETY OPEN SCIENCE　2　(5)　150120-150120　2015/05

    DOI： 10.1098/rsos.150120 　

    ISSN： 2054-5703

  55. Distinct dopamine neurons mediate reward signals for short- and long-term memories Peer-reviewed

    Nobuhiro Yamagata, Toshiharu Ichinose, Yoshinori Aso, Pierre-Yves Placais, Anja B. Friedrich, Richard J. Sima, Thomas Preat, Gerald M. Rubin, Hiromu Tanimoto

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　112　(2)　578-583　2015/01

    DOI： 10.1073/pnas.1421930112 　

    ISSN： 0027-8424

  56. Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila Peer-reviewed

    Yoshinori Aso, Divya Sitaraman, Toshiharu Ichinose, Karla R. Kaun, Katrin Vogt, Ghislain Belliart-Guerin, Pierre-Yves Placais, Alice A. Robie, Nobuhiro Yamagata, Christopher Schnaitmann, William J. Rowell, Rebecca M. Johnston, Teri-T B. Ngo, Nan Chen, Wyatt Korff, Michael N. Nitabach, Ulrike Heberlein, Thomas Preat, Kristin M. Branson, Hiromu Tanimoto, Gerald M. Rubin

    ELIFE　3　e04580-e04580　2014/12

    DOI： 10.7554/eLife.04580 　

    ISSN： 2050-084X

  57. The neuronal architecture of the mushroom body provides a logic for associative learning Peer-reviewed

    Yoshinori Aso, Daisuke Hattori, Yang Yu, Rebecca M. Johnston, Nirmala A. Iyer, Teri-T B. Ngo, Heather Dionne, L. F. Abbott, Richard Axel, Hiromu Tanimoto, Gerald M. Rubin

    ELIFE　3　e04577-e04577　2014/12

    DOI： 10.7554/eLife.04577 　

    ISSN： 2050-084X

  58. Shared mushroom body circuits underlie visual and olfactory memories in Drosophila Peer-reviewed

    Katrin Vogt, Christopher Schnaitmann, Kristina V. Dylla, Stephan Knapek, Yoshinori Aso, Gerald M. Rubin, Hiromu Tanimoto

    ELIFE　3　e02395-e02395　2014/08

    DOI： 10.7554/eLife.02395 　

    ISSN： 2050-084X

  59. Converging Circuits Mediate Temperature and Shock Aversive Olfactory Conditioning in Drosophila Peer-reviewed

    Dana Shani Galili, Kristina V. Dylla, Alja Luedke, Anja B. Friedrich, Nobuhiro Yamagata, Jin Yan Hilary Wong, Chien Hsien Ho, Paul Szyszka, Hiromu Tanimoto

    CURRENT BIOLOGY　24　(15)　1712-1722　2014/08

    DOI： 10.1016/j.cub.2014.06.062 　

    ISSN： 0960-9822

    eISSN： 1879-0445

  60. Color Discrimination with Broadband Photoreceptors Peer-reviewed

    Christopher Schnaitmann, Christian Garbers, Thomas Wachtler, Hiromu Tanimoto

    CURRENT BIOLOGY　23　(23)　2375-2382　2013/12

    DOI： 10.1016/j.cub.2013.10.037 　

    ISSN： 0960-9822

    eISSN： 1879-0445

  61. Two Pairs of Mushroom Body Efferent Neurons Are Required for Appetitive Long-Term Memory Retrieval in Drosophila Peer-reviewed

    Pierre-Yves Placais, Severine Trannoy, Anja B. Friedrich, Hiromu Tanimoto, Thomas Preat

    CELL REPORTS　5　(3)　769-780　2013/11

    DOI： 10.1016/j.celrep.2013.09.032 　

    ISSN： 2211-1247

  62. Suppression of Conditioned Odor Approach by Feeding Is Independent of Taste and Nutritional Value in Drosophila Peer-reviewed

    Franz Gruber, Stephan Knapek, Michiko Fujita, Koichiro Matsuo, Lasse Braecker, Nao Shinzato, Igor Siwanowicz, Teiichi Tanimura, Hiromu Tanimoto

    CURRENT BIOLOGY　23　(6)　507-514　2013/03

    DOI： 10.1016/j.cub.2013.02.010 　

    ISSN： 0960-9822

    eISSN： 1879-0445

  63. Short Neuropeptide F Acts as a Functional Neuromodulator for Olfactory Memory in Kenyon Cells of Drosophila Mushroom Bodies Peer-reviewed

    Stephan Knapek, Lily Kahsai, Asa M. E. Winther, Hiromu Tanimoto, Dick R. Nassel

    JOURNAL OF NEUROSCIENCE　33　(12)　5340-+　2013/03

    DOI： 10.1523/JNEUROSCI.2287-12.2013 　

    ISSN： 0270-6474

  64. Identification of a dopamine pathway that regulates sleep and arousal in Drosophila Peer-reviewed

    Taro Ueno, Jun Tomita, Hiromu Tanimoto, Keita Endo, Kei Ito, Shoen Kume, Kazuhiko Kume

    NATURE NEUROSCIENCE　15　(11)　1516-1523　2012/11

    DOI： 10.1038/nn.3238 　

    ISSN： 1097-6256

  65. A subset of dopamine neurons signals reward for odour memory in Drosophila Peer-reviewed

    Chang Liu, Pierre-Yves Placais, Nobuhiro Yamagata, Barret D. Pfeiffer, Yoshinori Aso, Anja B. Friedrich, Igor Siwanowicz, Gerald M. Rubin, Thomas Preat, Hiromu Tanimoto

    NATURE　488　(7412)　512-+　2012/08

    DOI： 10.1038/nature11304 　

    ISSN： 0028-0836

  66. Three Dopamine Pathways Induce Aversive Odor Memories with Different Stability Peer-reviewed

    Yoshinori Aso, Andrea Herb, Maite Ogueta, Igor Siwanowicz, Thomas Templier, Anja B. Friedrich, Kei Ito, Henrike Scholz, Hiromu Tanimoto

    PLOS GENETICS　8　(7)　2012/07

    DOI： 10.1371/journal.pgen.1002768 　

    ISSN： 1553-7404

  67. Three Dopamine pathways induce aversive odor memories with different stability Peer-reviewed

    Yoshinori Aso, Andrea Herb, Maite Ogueta, Igor Siwanowicz, Thomas Templier, Anja B. Friedrich, Kei Ito, Henrike Scholz, Hiromu Tanimoto

    PLoS Genetics　8　(7)　e1002768-e1002768　2012/07

    DOI： 10.1371/journal.pgen.1002768 　

    ISSN： 1553-7390 1553-7404

  68. Different classes of input and output neurons reveal new features in microglomeruli of the adult Drosophila mushroom body calyx Peer-reviewed

    Nancy J. Butcher, Anja B. Friedrich, Zhiyuan Lu, Hiromu Tanimoto, Ian A. Meinertzhagen

    JOURNAL OF COMPARATIVE NEUROLOGY　520　(10)　2185-2201　2012/07

    DOI： 10.1002/cne.23037 　

    ISSN： 0021-9967

  69. Slow oscillations in two pairs of dopaminergic neurons gate long-term memory formation in Drosophila Peer-reviewed

    Pierre-Yves Placais, Severine Trannoy, Guillaume Isabel, Yoshinori Aso, Igor Siwanowicz, Ghislain Belliart-Guerin, Philippe Vernier, Serge Birman, Hiromu Tanimoto, Thomas Preat

    NATURE NEUROSCIENCE　15　(4)　592-599　2012/04

    DOI： 10.1038/nn.3055 　

    ISSN： 1097-6256

  70. Event Timing in Associative Learning: From Biochemical Reaction Dynamics to Behavioural Observations Peer-reviewed

    Ayse Yarali, Johannes Nehrkorn, Hiromu Tanimoto, Andreas V. M. Herz

    PLOS ONE　7　(3)　e32885-e32885　2012/03

    DOI： 10.1371/journal.pone.0032885 　

    ISSN： 1932-6203

  71. Pan-Neuronal Knockdown of Calcineurin Reduces Sleep in the Fruit Fly, Drosophila melanogaster Peer-reviewed

    Jun Tomita, Madoka Mitsuyoshi, Taro Ueno, Yoshinori Aso, Hiromu Tanimoto, Yasuhiro Nakai, Toshiro Aigaki, Shoen Kume, Kazuhiko Kume

    JOURNAL OF NEUROSCIENCE　31　(37)　13137-13146　2011/09

    DOI： 10.1523/JNEUROSCI.5860-10.2011 　

    ISSN： 0270-6474

  72. Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila Peer-reviewed

    Julien Sejourne, Pierre-Yves Placais, Yoshinori Aso, Igor Siwanowicz, Severine Trannoy, Vladimiros Thoma, Stevanus R. Tedjakumala, Gerald M. Rubin, Paul Tchenio, Kei Ito, Guillaume Isabel, Hiromu Tanimoto, Thomas Preat

    NATURE NEUROSCIENCE　14　(7)　903-U129　2011/07

    DOI： 10.1038/nn.2846 　

    ISSN： 1097-6256

  73. Cellular site and molecular mode of synapsin action in associative learning Peer-reviewed

    Birgit Michels, Yi-chun Chen, Timo Saumweber, Dushyant Mishra, Hiromu Tanimoto, Benjamin Schmid, Olivia Engmann, Bertram Gerber

    LEARNING & MEMORY　18　(5)　332-344　2011/05

    DOI： 10.1101/lm.2101411 　

    ISSN： 1072-0502

    eISSN： 1549-5485

  74. Olfactory Trace Conditioning in Drosophila Peer-reviewed

    Dana Shani Galili, Alja Luede, C. Giovanni Galizia, Paul Szyszka, Hiromu Tanimoto

    JOURNAL OF NEUROSCIENCE　31　(20)　7240-7248　2011/05

    DOI： 10.1523/JNEUROSCI.6667-10.2011 　

    ISSN： 0270-6474

  75. Bruchpilot, A Synaptic Active Zone Protein for Anesthesia-Resistant Memory Peer-reviewed

    Stephan Knapek, Stephan Sigrist, Hiromu Tanimoto

    JOURNAL OF NEUROSCIENCE　31　(9)　3453-3458　2011/03

    DOI： 10.1523/JNEUROSCI.2585-10.2011 　

    ISSN： 0270-6474

  76. カルシニューリンによるショウジョウバエの睡眠および記憶の制御(Calcineurin regulates sleep and memory in Drosophila)

    冨田 淳, 光吉 まどか, 上野 太郎, 麻生 能功, 谷本 拓, 粂 昭苑, 粂 和彦

    神経化学　49　(2-3)　570-570　2010/08

    Publisher: (一社)日本神経化学会

    ISSN： 0037-3796

  77. Specific Dopaminergic Neurons for the Formation of Labile Aversive Memory Peer-reviewed

    Yoshinori Aso, Igor Siwanowicz, Lasse Braecker, Kei Ito, Toshihiro Kitamoto, Hiromu Tanimoto

    CURRENT BIOLOGY　20　(16)　1445-1451　2010/08

    DOI： 10.1016/j.cub.2010.06.048 　

    ISSN： 0960-9822

  78. Cellular Configuration of Single Octopamine Neurons in Drosophila Peer-reviewed

    Sebastian Busch, Hiromu Tanimoto

    JOURNAL OF COMPARATIVE NEUROLOGY　518　(12)　2355-2364　2010/06

    DOI： 10.1002/cne.22337 　

    ISSN： 0021-9967

  79. Synapsin is selectively required for anesthesia-sensitive memory Peer-reviewed

    Stephan Knapek, Bertram Gerber, Hiromu Tanimoto

    LEARNING & MEMORY　17　(2)　76-79　2010/02

    DOI： 10.1101/lm.1661810 　

    ISSN： 1072-0502

    eISSN： 1549-5485

  80. Appetitive and aversive visual learning in freely moving Drosophila Peer-reviewed

    Christopher Schnaitmann, Katrin Vogt, Tilman Triphan, Hiromu Tanimoto

    FRONTIERS IN BEHAVIORAL NEUROSCIENCE　4　10-10　2010

    DOI： 10.3389/fnbeh.2010.00010 　

    ISSN： 1662-5153

  81. A Map of Octopaminergic Neurons in the Drosophila Brain Peer-reviewed

    Sebastian Busch, Mareike Selcho, Kei Ito, Hiromu Tanimoto

    JOURNAL OF COMPARATIVE NEUROLOGY　513　(6)　643-667　2009/04

    DOI： 10.1002/cne.21966 　

    ISSN： 0021-9967

  82. The Mushroom Body of Adult Drosophila Characterized by GAL4 Drivers Peer-reviewed

    Yoshinori Aso, Kornelia Grubel, Sebastian Busch, Anja B. Friedrich, Igor Siwanowicz, Hiromu Tanimoto

    JOURNAL OF NEUROGENETICS　23　(1-2)　156-U29　2009

    DOI： 10.1080/01677060802471718 　

    ISSN： 0167-7063

  83. 'Pain relief' learning in fruit flies Peer-reviewed

    Ayse Yarali, Thomas Niewalda, Yi-Chun Chen, Hiromu Tanimoto, Stefan Duerrnagel, Bertram Gerber

    ANIMAL BEHAVIOUR　76　1173-1185　2008/10

    DOI： 10.1016/j.anbehav.2008.05.025 　

    ISSN： 0003-3472

  84. Neuronal assemblies of the Drosophila mushroom body Peer-reviewed

    Nobuaki K. Tanaka, Hiromu Tanimoto, Kei Ito

    JOURNAL OF COMPARATIVE NEUROLOGY　508　(5)　711-755　2008/06

    DOI： 10.1002/cne.21692 　

    ISSN： 0021-9967

  85. Multiple memory traces for olfactory reward learning in Drosophila Peer-reviewed

    Andreas S. Thum, Arnim Jenett, Kei Ito, Martin Heisenberg, Hiromu Tanimoto

    JOURNAL OF NEUROSCIENCE　27　(41)　11132-11138　2007/10

    DOI： 10.1523/JNEUROSCI.2712-07.2007 　

    ISSN： 0270-6474

  86. JDRC 8 - History, neurobiology and imaging Peer-reviewed

    Hiromu Tanimoto, Takashi Suzuki

    FLY　1　(5)　291-293　2007/09

    DOI： 10.4161/fly.5242 　

    ISSN： 1933-6934

  87. Light activation of an innate olfactory avoidance response in Drosophila Peer-reviewed

    Greg S. B. Suh, Shlomo Ben-Tabou de Leon, Hiromu Tanimoto, Andre Fiala, Seymour Benzer, David J. Anderson

    CURRENT BIOLOGY　17　(10)　905-908　2007/05

    DOI： 10.1016/j.cub.2007.04.046 　

    ISSN： 0960-9822

  88. Differential potencies of effector genes in adult Drosophila Peer-reviewed

    Andreas S. Thum, Stephan Knapek, Jens Rister, Eva Dierichs-Schmitt, Martin Heisenberg, Hiromu Tanimoto

    JOURNAL OF COMPARATIVE NEUROLOGY　498　(2)　194-203　2006/09

    DOI： 10.1002/cne.21022 　

    ISSN： 0021-9967

  89. A role for Synapsin in associative learning: The Drosophila larva as a study case Peer-reviewed

    Birgit Michels, Sören Diegelmann, Hiromu Tanimoto, Isabell Schwenkert, Erich Buchner, Bertram Gerber

    LEARNING & MEMORY　12　(3)　224-231　2005/05

    DOI： 10.1101/lm.92805 　

    ISSN： 1072-0502

  90. Pain-relief learning in fruit flies Peer-reviewed

    Ayse Yarali, Thomas Niewalda, Yi-chun Chen, Hiromu Tanimoto, Stefan Duerrnagel, Bertram Gerber

    REVIEWS IN THE NEUROSCIENCES　16　(76(4))　S69-S69　2005

    DOI： 10.1016/j.anbehav.2008.05.025 　

    ISSN： 0334-1763

  91. An engram found? - Evaluating the evidence from fruit flies Peer-reviewed

    Bertram Gerber, Hiromu Tanimoto, Martin Heisenberg

    CURRENT OPINION IN NEUROBIOLOGY　14　(6)　737-744　2004/12

    DOI： 10.1016/j.conb.2004.10.014 　

    ISSN： 0959-4388

  92. Event timing turns punishment to reward Peer-reviewed

    Hiromu Tanimoto, Martin Heisenberg, Bertram Gerber

    NATURE　430　(7003)　983-983　2004/08

    DOI： 10.1038/430983a 　

    ISSN： 0028-0836

  93. Androgen-dependent neurodegeneration by polyglutamine-expanded human androgen receptor in Drosophila Peer-reviewed

    Ken-ichi Takeyama, Saya Ito, Ayako Yamamoto, Hiromu Tanimoto, Takashi Furutani, Hirotaka Kanuka, Masayuki Miura, Tetsuya Tabata, Shigeaki Kato

    NEURON　35　(5)　855-864　2002/08

    DOI： 10.1016/S0896-6273(02)00875-9 　

    ISSN： 0896-6273

  94. Hedgehog creates a gradient of DPP activity in Drosophila wing imaginal discs Peer-reviewed

    Hiromu Tanimoto, Susumu Itoh, Peter ten Dijke, Tetsuya Tabata

    MOLECULAR CELL　5　(1)　59-71　2000/01

    DOI： 10.1016/S1097-2765(00)80403-7 　

    ISSN： 1097-2765

  95. Brinker is a target of Dpp in Drosophila that negatively regulates Dpp-dependent genes Peer-reviewed

    Maki Minami, Noriyuki Kinoshita, Yuko Kamoshida, Hiromu Tanimoto, Tetsuya Tabata

    NATURE　398　(6724)　242-246　1999/03

    DOI： 10.1038/18451 　

    ISSN： 0028-0836

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Misc. 24

  1. Jellyfish and flies shed light on the origin of appetite Invited

    Abe A., Thoma V., Tanimoto H.

    The Cell　56　(6)　24-27　2024/06

  2. 翻訳制御による細胞個性の実現と神経適応

    市之瀬敏晴, 市之瀬敏晴, 菅野舞, 菅野舞, 近藤周, 七野悠一, 水戸麻理, 岩崎信太郎, 岩崎信太郎, 谷本拓

    日本生物学的精神医学会(Web)　46th　2024

  3. Artificial selection on blue light toxicity induces symbiont-dependent phenotypic changes in Drosophila

    高田悠太, 西塔心路, 大坪和香子, 布施直之, 市之瀬敏晴, 谷本拓, 堀雅敏

    日本生態学会大会講演要旨(Web)　71st　2024

  4. 青色光毒性を用いた人為選抜が駆動したDrosophila melanogasterにおける”Acetobacter共生菌-脂質代謝に関する表現型”の進化

    高田悠太, 西塔心路, 大坪和香子, 布施直之, 市之瀬敏晴, 市之瀬敏晴, 谷本拓, 堀雅敏

    日本応用動物昆虫学会大会講演要旨　68th　2024

  5. Regulation of gut starvation response through Drosophila NP3253 neurons International-journal

    NGUYEN Quang-Dat, 藤井希帆, 石橋謙太朗, 布施直之, 大坪和香子, 北澤春樹, 谷本拓, 倉田祥一朗

    日本分子生物学会年会プログラム・要旨集(Web)　46th　(2)　e70005　2023

    DOI： 10.1111/gtc.70005 　

    More details Close

    The "gut-brain axis," a bidirectional communication system between the gastrointestinal tract and the central nervous system, plays a crucial role in regulating complex physiological functions in response to nutrients, pathogens, and microbiota. However, the cellular and molecular mechanisms governing this regulation remain poorly understood. Using Drosophila melanogaster as a model organism, we previously identified NP3253 neurons, located in both the brain and gut, as key contributors to gut homeostasis during oral bacterial infection. Here, we found a novel role of NP3253 neurons in regulating starvation resistance. We observed that a subset of NP3253 neurons in the gut were activated during starvation. To investigate downstream effect, we conducted RNA-Seq analysis on the gut of adult flies with genetically silenced NP3253 neurons, comparing gene expression under starved and fed conditions. This analysis identified 26 genes differentially expressed in response to both starvation and NP3253 neuronal activity. Among these, CG12643, encoding an uncharacterized short peptide, was found to be essential for starvation resistance in the gut. Our findings demonstrate that NP3253 neurons modulate the gut gene expression in response to starvation, thereby supporting physiological adaptation to environmental stressors.

  6. Regulation of starvation response through neural control in Drosophila

    藤井希帆, NGUYEN Quang-Dat, 石橋謙太朗, 布施直之, 谷本拓, 北澤春樹, 大坪和香子, 倉田祥一朗

    日本分子生物学会年会プログラム・要旨集(Web)　46th　2023

  7. 適正な「価値判断」を制御する神経回路

    谷本 拓

    上原記念生命科学財団研究報告集　32　4p　2018

    Publisher: 上原記念生命科学財団

    ISSN： 2433-3441

  8. Suppression of Conditioned Odor Approach by Feeding Is Independent of Taste and Nutritional Value in Drosophila (vol 23, pg 507, 2013)

    Franz Gruber, Stephan Knapek, Michiko Fujita, Koichiro Matsuo, Lasse Braecker, Nao Shinzato, Igor Siwanowicz, Teiichi Tanimura, Hiromu Tanimoto

    CURRENT BIOLOGY　23　(7)　631-631　2013/04

    DOI： 10.1016/j.cub.2013.03.059 　

    ISSN： 0960-9822

  9. Reward circuit in the fly brain

    Nobuhiro Yamagata, Yoshinori Aso, Hiromu Tanimoto

    Experimental Medicine　3　(2月)　429-432　2013/02

  10. 報酬・罰を伝達するドーパミン神経と記憶の局所回路

    谷本 拓

    生体の科学　64　(64)　348-353　2013

    Publisher: 金原一郎記念医学医療振興財団 ; 1949-

    DOI： 10.11477/mf.2425101469 　

    ISSN： 0370-9531

  11. NMDA receptor-calcineurin signaling function in promoting sleep in Drosophila

    Jun Tomita, Madoka Mitsuyoshi, Taro Ueno, Yoshinori Aso, Hiromu Tanimoto, Shoen Kume, Kazuhiko Kume

    JOURNAL OF NEUROGENETICS　26　57-57　2012/12

    ISSN： 0167-7063

  12. Differential dopaminergic circuits for the formation of olfactory memory in Drosophila

    Hiromu Tanimoto, Yoshinori Aso, Igor Siwanowicz, Lasse Braecker, Chang Liu, Kei Ito, Toshihiro Kitamoto

    NEUROSCIENCE RESEARCH　68　E26-E26　2010

    DOI： 10.1016/j.neures.2010.07.353 　

    ISSN： 0168-0102

  13. Calcineurin regulates sleep and memory in Drosophila

    Jun Tomita, Madoka Mitsuyoshi, Taro Ueno, Yoshinori Aso, Hiromu Tanimoto, Shoen Kume, Kazuhiko Kume

    NEUROSCIENCE RESEARCH　68　E176-E176　2010

    DOI： 10.1016/j.neures.2010.07.2355 　

    ISSN： 0168-0102

  14. Requirement of the mushroom body associated neurons for appetitive and aversive odour memories in Drosophila

    Yoshinori Aso, Ito Kei, Martin Heisenberg, Hiromu Tanimoto

    JOURNAL OF NEUROGENETICS　23　S48-S48　2009

    ISSN： 0167-7063

  15. Behavioural assay for appetitive visual memory

    Christopher Schnaitmann, Tilman Triphan, Simon Triphan, Martin Heisenberg, Hiromu Tanimoto

    JOURNAL OF NEUROGENETICS　23　S78-S79　2009

    ISSN： 0167-7063

  16. A map of single octopaminergic neurons in the Drosophila brain

    Sebastian Busch, Mareike Selcho, Kei Ito, Hiromu Tanimoto

    JOURNAL OF NEUROGENETICS　23　S49-S49　2009

    ISSN： 0167-7063

  17. Individual dopaminergic neurons signalling punishment in associative odor memory

    Hiromu Tanimoto, Yoshinori Aso, Kei Ito

    NEUROSCIENCE RESEARCH　65　S25-S25　2009

    DOI： 10.1016/j.neures.2009.09.1630 　

    ISSN： 0168-0102

  18. Requirement of presynaptic proteins for specific components of associative memory

    Stephan Knapek, Eva Dierich-Schmitt, Bertram Gerber, Stephan Sigrist, Erich Buchner, Martin Heisenberg, Hiromu Tanimoto

    JOURNAL OF NEUROGENETICS　23　S40-S40　2009

    ISSN： 0167-7063

  19. Toward localizing a synapsin-dependent olfactory memory trace in the brain of larval Drosophila

    Birgit Michels, Bertram Gerber, Hiromu Tanimoto, Erich Buchner

    JOURNAL OF NEUROGENETICS　20　(3-4)　178-179　2006/07

    ISSN： 0167-7063

  20. Specific requirement of synapsin-dependent synaptic plasticity for short-term memory

    Stephan Knapek, Eva Dierich-Schmi, Martin Heisenberg, Bertram Gerber, Hiromu Tanimoto

    JOURNAL OF NEUROGENETICS　20　(3-4)　146-147　2006/07

    ISSN： 0167-7063

  21. Multiple memories of Drosophila sugar reward learning

    Andreas S. Thum, Martin Heisenberg, Hiromu Tanimoto

    JOURNAL OF NEUROGENETICS　20　(3-4)　238-239　2006/07

    ISSN： 0167-7063

  22. A role of the presynaptic protein synapsin in associative learning

    Birgit Michels, Sören Diegelmann, Hiromu Tanimoto, Isabell Schwenkert, Erich Buchner, Bertram Gerber

    REVIEWS IN THE NEUROSCIENCES　16　S47-S47　2005

    ISSN： 0334-1763

  23. Hedgehogはコンパートメント境界の形成とそこに発現するモルフォゲンの活性勾配を制御する (特集 位置情報と境界形成を担うシグナル:器官形成の基本原理)

    武井ゆき, 谷本拓, 多羽田哲也

    細胞工学　21　(5)　508-512　2002/05

    Publisher: 秀潤社

    ISSN： 0287-3796

  24. Genetic screen to identify novel genes involved in pattern formation of the developing Drosophila wing

    TANIMOTO Hiromu, TABATA Tetsuya

    21　626-626　1998/12/01

Show all ︎Show first 5

Books and Other Publications 1

  1. 分子昆虫学―ポストゲノムの昆虫研究―

    谷本 拓

    共立出版　2009

    ISBN: 9784320056954

Presentations 105

  1. Piezo mediating ingestion-induced aversion memory in satiated Drosophila

    Nobuhiro Takahashi, Hiromu Tanimoto, Nobuhiro Yamagata

    The 4th Asia-Pacific Drosophila Neurobiology Conference　2026/04/21

  2. Disruption of a Selective Vesicle Pool upon Retrograde Amnesia Dissociates Memory at Presynaptic Terminals

    Hiromu Tanimoto

    The 4th Asia-Pacific Drosophila Neurobiology Conference　2026/04/23

  3. Differential regulation of synaptic vesicles underlies labile and consolidated memories of Drosophila Invited

    Hiromu Tanimoto

    2025/12/23

  4. Satiety-gated aversive ingestion memory mediated by Piezo in Drosophila

    Nobuhiro Takahashi, Hiromu Tanimoto, Nobuhiro Yamagata

    The 47th Annual Meeting of The Japanese Society for Comparative Physiology and Biochemistry　2025/12/05

  5. Activity-Dependent Translational Dynamics of Ribosomal Proteins

    Akihiro Sasaya, Masaki Kato, Shu Kondo, Hiromu Tanimoto

    The 48th Annual Meeting of the Molecular Biology Society of Japan　2025/12/03

  6. Molecular mechanisms of differential translation among neuronal and glial cells

    Yingyue Fang, Hiromu Tanimoto., Toshiharu Ichinose

    2025/12/02

  7. クラゲの栄養素依存的摂食抑制

    眞柴 弦太, 谷本 拓, トーマ ブラディミロス

    2025年度生理研研究会 食理学研究会　2025/10/17

  8. Neuromodulation of odor-guided feeding motivation and odor preference in the hungry fruit fly Drosophila melanogaster

    Tzu-Ting Huang, Hiromu Tanimoto

    The 19th European Symposium for Insect Taste and Olfaction　2025/09/19

  9. Differential regulation of synaptic vesicles underlies labile and consolidated memories of Drosophila Invited

    Hiromu Tanimoto

    The NIPS Research Meeting 2025 "Frontiers in Memory and Learning Research through Multilayered Approaches"　2025/09/18

  10. Proteins synthesized upon neuronal stimulation Invited

    Hiromu Tanimoto

    The Future of Bee and Fly Neuroethology, Modeling and Robotics　2025/08/25

  11. Uncovering genetic control of food-searching motivation and odor preference in the fruit fly Drosophila melanogaster

    Tzu-Ting Huang, Hiromu Tanimoto

    The 48th Annual Meeting of the Japan Neuroscience Society　2025/07/24

  12. Molecular mechanisms of differential protein synthesis among cell types

    Yingyue Fang, Hiromu Tanimoto, Toshiharu Ichinose

    26th Annual Meeting of the RNA Society of Japan　2025/07/09

  13. On the evolutionary origin of the central nervous system Invited

    Hiromu Tanimoto

    ACC International Symposium 2025　2025/01/25

  14. Foregut Piezo neurons convey sugar aversion in fed Drosophila

    Nobuhiro Takahashi, Hiromu Tanimoto, Nobuhiro Yamagata

    the 46th Annual Meeting of the Japan Society for Comparative Physiology and Biochemistry　2024/09/30

  15. Imbalance between dopamine subsystems underlies optimism bias

    Nobuhiro Yamagata, Nobuhiro Takahashi, Norihiro Katayama, Hiromu Tanimoto

    16th Japanese Drosophila Research Conference　2024/09/17

  16. Foregut Piezo reinforces aversive sugar memory

    Nobuhiro Takahashi, Hiromu Tanimoto, Nobuhiro Yamagata

    16th Japanese Drosophila Research Conference　2024/09/17

  17. Molecular mechanisms underlying the untranslated region（UTR）- mediated differential translational regulation in neurons and glia

    Yingyue Fang, Hiromu Tanimoto, Toshiharu Ichinose

    16th Japanese Drosophila Research Conference　2024/09/18

  18. Regulation of synaptic vesicles underlies retrograde amnesia

    Kaito Kabetani, Shun Hiramatsu, Shu Kondo, Hiromu Tanimoto

    16th Japanese Drosophila Research Conference　2024/09/18

  19. Octopamine signaling regulates intracellular active zone heterogeneity depending on nutritional states

    Sayaka Eno, Hongyang Wu, Shu Kondo, Kokoro Saito, Kyoko Jinnai, Nobuhiro Yamagata, Hiromu Tanimoto

    16th Japanese Drosophila Research Conference　2024/09/17

  20. Toward the evolutionary origin of appetite systems Invited

    Hiromu Tanimoto

    The 95th Annual Meeting of the Zoological Society of Japan　2024/09/13

  21. Octopamine signaling regulates intracellular active zone heterogeneity depending on nutritional states

    Sayaka Eno, Hongyang Wu, Kokoro Saito, Kyoko Jinnai, Shu Kondo, Hiromu Tanimoto

    Neurofly 2024　2024/09/06

  22. Intracellular Profiling of Synapses in Identified Neurons in the Drosophila brains

    Hongyang Wu, Yoh Maekawa, Sayaka Eno, Shu Kondo, Nobuhiro Yamagata, Hiromu Tanimoto

    NEURO 2024　2024/07/24

  23. Activity imbalance between rewarding and punishment dopamine subsystems underlies optimism bias

    Nobuhiro Yamagata, Nobuhiro Takahashi, Norihiro Katayama, Hiromu Tanimoto

    NEURO 2024　2024/07/27

  24. Cell type distinction and neuronal adaptation through translational regulation

    Toshiharu Ichinose, Mai Kanno, Shu Kondo, Yuichi Shichino, Mari Mito, Shintaro Iwasaki, Hiromu Tanimoto

    NEURO 2024　2024/07/26

  25. Toward the evolutionary origin of appetite systems Invited

    Hiromu Tanimoto

    Neuro 2024　2024/07/26

  26. Learning from Cladonema feeding regulation toward the evolutionary origins of nervous systems Invited

    Hiromu Tanimoto, Vladimiros Thoma, MIngxin Liu

    2024/03/28

  27. On the origin of appetite: Evidence from functional complementation in flies and jellyfish

    Vladimiros Thoma, Shuhei Sakai, Koki Nagata, Yuu Ishii, Shinichiro Maruyama, Ayako Abe, Shu Kondo, Masakado Kawata, Shun Hamada, Ryusaku Deguchi, Hiromu Tanimoto

    The 3rd Asia Pacific Drosophila Neurobiology Conference　2024/02/27

  28. Tuning the reward intensity Invited

    Hiromu Tanimoto

    The 3rd Asia Pacific Drosophila Neurobiology Conference　2024/02/28

  29. Regulation of vesicle pools for distinct memory phases Invited

    Hiromu Tanimoto

    NIPS Learning & Memory meeting 2023 “Diverse approaches to understanding learning and memory”　2023/09/26

  30. A novel role of Ecdysone-DopEcR signaling in toxin aversion and addiction-like behavior

    Kokoro Saito, Aoi Watanabe, Hiromu Tanimoto, Toshiharu Ichinose

    The 46th Annual Meeting of the Japan Neuroscience Society　2023/08/03

  31. Presynaptic nicotinic receptor in dopamine neurons mediates learned odor choice

    Nobuhiro Yamagata, Rino Ichikawa, Ayako Abe, Norihiro Katayama, Hiromu Tanimoto

    The 46th Annual Meeting of the Japan Neuroscience Society　2023/08/03

  32. On the origin of appetite: GLWamide in jellyfish represents an ancestral satiety neuropeptide

    Vladimiros Thoma, Shuhei Sakai, Koki Nagata, Yuu Ishii, Shinichiro Maruyama, Ayako Abe, Shu Kondo, Masakado Kawata, Shun Hamada, Ryusaku Deguchi, Hiromu Tanimoto

    EMBO/COB workshop 'Trans-Scale Biology' using exotic non-model organisms　2023/07/25

  33. The state-dependent active zone structural plasticity induced through octopamine signalling

    Sayaka Eno, Hongyang Wu, Kokoro Saito, Kyoko Jinnai, Shu Kondo, Hiromu Tanimoto

    Asia Pacific Drosophila Research Conference 2023　2023/07/26

  34. Feedback regulation of dopamine signaling tunes reward intensities.

    Kokoro Saito, Shun Hiramatsu, Aoi Watanabe, Toshiharu Ichinose, Nobuhiro Yamagata, Hiromu Tanimoto

    Mushroom body meeting 2023　2023/05/30

  35. On the origin of appetite: GLWamide in jellyfish represents an ancestral satiety neuropeptide Invited

    Hiromu Tanimoto

    The 19th International Symposium on Molecular and Neural mechanisms of Taste and Olfactory Perception　2023/03/19

  36. Synaptic heterogeneity among the compartments ofaxon terminals

    Hongyang Wu, Sayaka Eno, Kokoro Saito, Shu Kondo, Nobuhiro Yamagata, Hiromu Tanimoto

    64th Anuual Drosophila Research Conference　2023/03/02

  37. Cell-type-specific protein visualization reveals subcellular localization and dynamics of endogenous dopamine receptors

    Shun Hiramatsu, Shu Kondo, Hidetaka Katow, Nobuhiro Yamagata, Hiromu Tanimoto

    64th Anuual Drosophila Research Conference　2023/03/02

  38. Activity dependent protein translation in the fly brain

    Toshiharu Ichinose, Hiromu Tanimoto

    ACC International Symposium 2023　2023/02/22

  39. Synapse heterogeneity among the compartments of axon terminals Invited

    Hiromu Tanimoto

    2023/02/03

  40. On the origin of appetite Invited

    谷本 拓

    NIBB新規モデル生物開発共同利用研究ミニシンポジウム「エダアシクラゲを用いた新規刺胞動物モデルの研究基盤構築」　2022/11/15

  41. Diversity of presynaptic protein localization within and among cell types

    Sayaka Eno, Hongyang Wu, Shu Kondo, Hiromu Tanimoto

    15th Japan Drosophila Research Conference　2022/09/12

  42. Translational regulation through upstream ORF ensures cell type specific expression of neuronal proteins

    Toshiharu Ichinose, Shu Kondo, Mai Kanno, Yuichi Shichino, Mari Mito, Shintaro Iwasaki, Hiromu Tanimoto

    15th Japan Drosophila Research Conference　2022/09/14

  43. A gradient synaptic heterogeneity along axon terminals of Kenyon cells

    Hongyang Wu, Shu Kondo, Sayaka Eno, Nobuhiro Yamagata, Hiromu Tanimoto

    Neurofly 2022　2022/09/07

  44. Dopamine auto-feedback regulation fine-tunes reward memory in Drosophila

    Kokoro Saito, Shun Hiramatsu, Hiromu Tanimoto

    European Chemoreception Research Organization 2022　2022/09/01

  45. Regulation of dopamine neurons for proper valuation Invited

    Nobuhiro Yamagata, Hiromu Tanimoto

    XXVI International Congress of Entomology　2022/07/19

  46. A gradient synaptic heterogeneity along axon terminals of Kenyon cells

    Hongyang Wu, Shu Kondo, Sayaka Eno, Nobuhiro Yamagata, Hiromu Tanimoto

    NEURO 2022　2022/07/01

  47. Dopamine auto-feedback regulation fine-tunes reward memory in Drosophila

    Kokoro Saito, Shun Hiramatsu, Hiromu Tanimoto

    NEURO 2022　2022/07/01

  48. Terminal activity of dopamine neurons controls reward signals in the fly Invited

    Hiromu Tanimoto

    2021/12/13

  49. Cell-type specific characterization of presynaptic active zone heterogeneity

    Hongyang Wu, Shu Kondo, Nobuhiro Yamagata, Hiromu Tanimoto

    14th Japan Drosophila Research Conference　2021/09/13

  50. Cav 2.2 channel cacophony regulates spontaneous and evoked active-zone calcium activity in dopamine terminals

    Karin Yamaguchi, Hiromu Tanimoto, Nobuhiro Yamagata

    14th Japan Drosophila Research Conference　2021/09/13

  51. Cell-type specific profiling of protein synthesis Invited

    Tanimoto H.

    2020/12/01

  52. Cell-type specific visualization of endogenous dopamine receptors

    Shun Hiramatsu, Takahiro Takahashi, Shu Kondo, Hidetaka Katow, Nobuhiro Yamagata N, Hiromu Tanimoto

    The 5th Asia Pacific Drosophila Research Conference　2020/01/06

  53. Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors. Invited

    Hiromu Tanimoto

    The 5th Asia Pacific Drosophila Research Conference　2020/01/07

  54. Disinhibition of rewarding dopamine neurons causes cognitive bias International-presentation Invited

    Nobuhiro Yamagata, Takahiro Takahashi, Rino Ichikawa, Hiromu Tanimoto

    The 18th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception　2019/11/02

  55. Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors International-presentation Invited

    Shu Kondo, Takahiro Takahashi, Yasuhito Imanishi, Hidetaka Katow, Shun Hiramatsu, Ayako Abe, Ajayrama Kumaraswamy, Nobuhiro Yamagata, Hiromu Tanimoto

    The 48th Naito Conference 'Integrated Sensory Sciences - Pain, Itch, Smell and Taste　2019/10/10

  56. Chronic malnutrition enhances preference for rewarding substances International-presentation

    Toshiharu Ichinose, Mai Kanno, Shu Kondo, Shun Hiramatsu, Ayako Abe, Sena Hatori, Riho Kobayashi, Kazuhiko Kume, Hiromu Tanimoto

    The 42nd Annual Meeting of the Japan Neuroscience Society　2019/07/27

  57. Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors.

    Shu Kondo, Takahiro Takahashi, Yasuhito Imanishi, Hidetaka Katow, Shun Hiramatsu, Katrina Lynn, Ayako Abe, Ajayrama Kumaraswamy, Nobuhiro Yamagata, Hiromu Tanimoto

    Gordon Research Conference 2019 - Modulation of Neural Circuits and Behavior　2019/05/27

  58. Quantification of Aggregation and Associated Brain Areas in Drosophila Melanogaster. International-presentation

    Takuto Okuno, Koichi Hashimoto, Hiromu Tanimoto

    IEEE International Conference on Pervasive Computing and Communications 2019　2019/03/11

  59. Chronic poor condition enhances preference to rewarding substances through dopamine system. International-presentation

    Toshiharu Ichinose, Mai Kanno, Shu Kondo, Shun Hiramatsu, Ayako Abe, Hiromu Tanimoto

    Asia Pacific Drosophila Neuroscience Conference 2019　2019/01/17

  60. Consolidation of appetitive long-term memory through dopamine-Raf signaling. International-presentation

    Huan Sun, Tomoki Nishioka, Toshiharu Ichinose, Kozo Kaibuchi, Hiromu Tanimoto

    Asia Pacific Drosophila Neuroscience Conference 2019　2019/01/17

  61. Memory formation by dopamine signals in the fly brain. Invited

    Hiromu Tanimoto

    昆虫脳コネクトーム研究の最前線公開シンポジウム　2018/12/03

  62. Neural circuits determining the predictive value of an odor International-presentation Invited

    Nobuhiro Yamagata, Hiromu Tanimoto

    The 17th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception　2018/12/01

  63. ドーパミン神経の活動修飾と報酬シグナル Invited

    Hiromu Tanimoto

    The 52nd Annual Meeting of the Japanese Association for the Study of Taste and Smell　2018/10/30

  64. Cell-type specific visualization of endogenous dopamine receptors.

    Shun Hiramatsu, Takahiro Takahashi, Shu Kondo, Hidetaka Katow, Nobuhiro Yamagata, Hiromu Tanimoto

    13th Japanese Drosophila Research Conference　2018/09/11

  65. Chronic poor condition enhances preference for rewarding substances.

    Toshiharu Ichinose, Mai Kanno, Ayako Abe, Hiromu Tanimoto

    13th Japanese Drosophila Research Conference　2018/09/11

  66. Neurochemical substrates underlying optimistic behavioral traits.

    Nobuhiro Yamagata, Hiromu Tanimoto

    The 41st Annual Meeting of the Japan Neuroscience Society　2018/07/26

  67. Neural circuits that distinguish memory processes in the fly brain International-presentation

    Mai Kanno, Toshiharu Ichinose, Hiromu Tanimoto

    International Symposium on Adaptive Circuit Shift 2017　2017/12/18

  68. Molecules functioning downstream of dopamine signaling for olfactory associative learning in the mushroom body

    Huan Sun, Shun Hiramatsu, Shu Kondo, Toshiharu Ichinose, Hiromu Tanimoto

    The Inaugural Asia-Pacific Drosophila Neurobiology Conference　2017/10/26

  69. Determination of reward values by regulation of dopamine neurons International-presentation Invited

    Hiromu Tanimoto

    The Inaugural Asia-Pacific Drosophila Neurobiology Conference　2017/10/26

  70. Interaction between GABA and dopamine underlies optimistic behavioral traits

    Nobuhiro Yamagata, Hiromu Tanimoto

    The 44th Naito ConferenceThe 44th Naito Conference 'Decision Making in the Brain - Motivation, Prediction, and Learning'　2017/10/04

  71. Segmentation of dopamine signaling in the fly brain International-presentation Invited

    Shu Kondo, Yasuhito Imanishi, Takahiro Takahashi, Hidetaka Katow, Ayako Abe, Nobuhiro Yamagata, Hiromu Tanimoto

    The 44th Naito Conference 'Decision Making in the Brain - Motivation, Prediction, and Learning'　2017/10/04

  72. State dependent feeding control by a nutrient signal

    Nobuhiro Yamagata, Takahiro Takahashi, Shu Kondo, Yasuhito Imanishi, Hiromu Tanimoto

    The 39th Annual Meeting of The Japanese Society for Comparative Physiology and Biochemistry　2017/09/25

  73. Frequency analysis of behavioral time series in temporal conditioning International-presentation

    Masayoshi Ikarashi, Hiromu Tanimoto

    The 2nd International Symposium on the Science of Mental Time　2017/09/12

  74. Classification of the direction and behavior of fruit flies by machine vision and image-based CNN International-presentation

    Takuto Okuno, Masayoshi Ikarashi, Pudith Sirigrivatanawong, Koichi Hashimoto, Hiromu Tanimoto

    Neuro Informatics 2017　2017/08/20

  75. Segmentation of dopamine signaling in the fly brain International-presentation Invited

    Hiromu Tanimoto

    Neuroscience Program of Academia Sinica Symposium on Drosophila Neurobiology　2017/07/26

  76. Memory by dopamine signals in the mushroom body International-presentation Invited

    Hiromu Tanimoto

    The EMBO-kavli meeting "Neural circuits and behaviour of Drosophila"　2017/07/06

  77. Segmentation of dopamine signaling in the fly brain

    Hiromu Tanimoto

    16th TMIMS International Symposium 'Functions and mechanisms of neuromodulation: a synthesis of knowledge from various organisms'　2017/05/17

  78. Molecules functioning downstream of dopamine signaling for olfactory associative learning in the mushroom body International-presentation

    Huan Sun, Toshiharu Ichinose, Nobuhiro Yamagata, Ayako Abe, Hiromu Tanimoto

    The 4th Asia-Pacific Drosophila Research Conference　2017/05/08

  79. Suppression of dopamine neurons mediates reward

    Nobuhiro Yamagata, Makoto Hiroi, Shu Kondo, Ayako Abe, Hiromu Tanimoto

    Janelia Farm Conference “Structure and Function of the Insect Mushroom Body”　2017/03/06

  80. Visualization and neuronal control of memory-guided choice behaviour. International-presentation Invited

    Mai Kanno, Toshiharu Ichinose, Yuya Onodera, Pudith Sirigrivatanawong, Koichi Hashimoto, Nobuhiro Yamagata, Hiromu Tanimoto

    Janelia Farm Conference “Structure and Function of the Insect Mushroom Body”　2017/03/08

  81. Temporal conditioning in insects

    Masayoshi Ikarashi, Hiromu Tanimoto

    Time In Tokyo: International Symposium on temporal perception and experience　2016/10/11

  82. Mapping dopamine receptor expression in the fly brain

    Yasuhito Imanishi, Nobuhiro Yamagata, Ayako Abe, Shu Kondo, Hiromu Tanimoto

    The 12th Japanese Drosophila Research Conference　2016/09/10

  83. Suppression of Dopamine Neurons Mediates Reward Invited

    Nobuhiro Yamagata, Makoto Hiroi, Shu Kondo, Ayako Abe, Hiromu Tanimoto

    Neurofly 2016　2016/09/05

  84. Functional dissociation in sweet taste recepter neurons between and within taste organs of Drosophila International-presentation

    Vladimiros Thoma, Stephan Knapek, Shogo Arai, Martin Hartl, Hiroshi Kohsaka, Pudith Sirigrivatanawong, Ayako Abe, Koichi Hashimoto, Hiromu Tanimoto

    17th International Symposium on Olfaction and Taste　2016/06/06

  85. Neural pathways for the formation, consolidation, and retrieval of memory in the fly brain International-presentation Invited

    Hiromu Tanimoto

    The 6th International Symposium on "Biology of Decision Making"　2016/05/27

  86. Reward signal in a recurrent circuit drives appetitive long-term memory formation

    Toshiharu Ichinose, Yoshinori Aso, Nobuhiro Yamagata, Gerald M. Rubin, Hiromu Tanimoto

    包括脳科学研究推進支援ネットワーク冬のシンポジウム　2015/12/17

  87. 嗅覚連合記憶の異なる素過程におけるキノコ体出力パターンの解析

    2015/12/03

  88. Suppression of dopamine neurons mediates reward

    Nobuhiro Yamagata, Makoto Hiroi, Shu Kondo, Ayako Abe, Tetsuya Tabata, Hiromu Tanimoto

    Cold Spring Harbor Laboratory Meeting 'Neurobiology of Drosophila'　2015/10/03

  89. Dopamine circuits and memory formation International-presentation Invited

    Hiromu Tanimoto

    14th European Symposium for Insect Taste and Olfaction　2015/09/20

  90. ショウジョウバエキノコ体の神経接続と記憶形成の回路 Invited

    谷本 拓

    日本動物学会第86回大会　2015/09/18

  91. Reward signal in a recurrent circuit drives appetitive long-term memory formation International-presentation Invited

    Hiromu Tanimoto

    International Symposium on the Science of Mental time　2015/09/12

  92. Mushroom body reward circuits International-presentation Invited

    Hiromu Tanimoto

    The EMBO-Kavli Workshop on Neural Circuits and Behaviour of Drosophila　2015/07/09

  93. Dopamine circuits for memory formation International-presentation Invited

    Hiromu Tanimoto

    3rd Asia-Pacific Drosophila Research Conference　2015/05/14

  94. Mushroom body circuits and memory formation. International-presentation Invited

    Hiromu Tanimoto

    The Genetics Society Autumn Meeting 2014　2014/11/27

  95. Functional dissociation in sweet taste receptor neurons between and within taste organs of Drosophila.

    Vladimiros Thoma, Stephan Knapek, Martin Hartl, Hiromu Tanimoto

    Neurofly 2014　2014/10/08

  96. Mushroom body circuits and memory formation. International-presentation Invited

    Hiromu Tanimoto

    Neurofly 2014　2014/10/08

  97. Reward signal in a recurrent circuit drives appetitive long-term memory formation

    Toshiharu Ichinose, Yoshinori Aso, Nobuhiro Yamagata, Ayako Abe, Gerald M. Rubin, Hiromu Tanimoto

    Janelia Conference 'Learning and Memory: A Synthesis of Bees and Flies'　2014/09/22

  98. Distinct dopamine neurons mediate reward signals for short- and long-term memories. International-presentation Invited

    Hiromu Tanimoto

    Janelia Farm 2014 FaJanelia Farm 2014 Fall Conference 'Learning and Memory: A Synthesis of Bees and Flies'll Conference　2014/09/22

  99. Distinct dopamine neurons mediate reward signals for short- and long-term memories

    Nobuhiro Yamagata, Toshiharu Ichinose, Hiromu Tanimoto

    The 85th Annual Meeting of the Zoological Society of Japan　2014/09/11

  100. Neural circuits for memory formation. International-presentation Invited

    Hiromu Tanimoto

    The 85th Annual Meeting of the Zoological Society of Japan　2014/09/11

  101. Neural circuits for colour discrimination learning in the fly Invited

    Hiromu Tanimoto, Katrin Vog, Christopher Schnaitmann, Yoshinori Aso, Gerald M. Rubin, Thomas Wachtler, Christian Garbers

    2014 ICN / JSCPB　2014/07/29

  102. Distinct reward signals drive short-term and long-term memories

    Nobuhiro Yamagata, Toshiharu Ichinose, Pierre-Yves Plaçais, Yoshinori Aso, Anja B. Friedrich, Richard J. Sima, Thomas Prea, Gerald M. Rubin, Hiromu Tanimoto

    2014 ICN / JSCPB　2014/07/31

  103. Distinct reward signals drive short-term and long-term memories

    Nobuhiro Yamagata, Toshiharu Ichinose, Pierre-Yves Plaçais, Yoshinori Aso, Anja B. Friedrich, Richard J. Sima, Thomas Preat, Gerald M. Rubin, Hiromu Tanimoto

    The 11th Japanese Drosophila Research Conference　2014/06/06

  104. Functional diversity of the Drosophila mushroom body. International-presentation Invited

    Hiromu Tanimoto

    Janelia Farm 2014 Spring Conference　2014/04/27

  105. High-throughput synapse profiling reveals cell-type-specific spatial configurations in the fly brain

    Hongyang Wu, Yoh Maekawa, Sayaka Eno, Shu Kondo, Nobuhiro Yamagata, Hiromu Tanimoto

    CSHL meeting 2025 "Neurobiology of Drosophila"　2025/10/07

Show all Show first 5

Research Projects 18

  1. Neural Mechanisms for the Regulation of Experience-Dependent Social Interaction

    Hiromu Tanimoto, Susumu Yokota, Masayuki Koganezawa

    Offer Organization: Tohoku University

    System: Frontier Research in Duo

    Institution: Tohoku University

    2025/06 - 2030/03

  2. 相反する記憶を駆動する翻訳メカニズムの解明

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(A)

    Institution: 東北大学

    2025/04/01 - 2028/03/31

  3. Exploring the diversity of adaptation from translatome dynamics

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Transformative Research Areas (A)

    Institution: Tohoku University

    2024/04/01 - 2026/03/31

  4. Translatome responses to extreme sleep rhythms and neuronal integration

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Institution: Tohoku University

    2022/10/07 - 2026/03/31

  5. トランスラトームダイナミクスから探る適応の多様性

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 学術変革領域研究(A)

    Category: 学術変革領域研究(A)

    Institution: 東北大学

    2022/06/16 - 2024/03/31

  6. 細胞内ナノドメインでのカルシウム動態の制御と機能的意義

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2022/05 - 2024/03

  7. 学習依存的に合成されるタンパク質の同定と記憶の長期化における動態の解明

    谷本 拓, 市之瀬 敏晴, 近藤 周

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(A)

    Category: 基盤研究(A)

    Institution: 東北大学

    2020/04/01 - 2023/03/31

    More details Close

    今から50年以上前、学習後のタンパク質合成が記憶の安定化に重要であることが報告された。しかし、翻訳されるべきタンパク質が何であり、神経系のどの細胞でどのように機能することで記憶が長期化するかは未解明のままである。 本研究はショウジョウバエの匂い報酬記憶をモデルに、記憶の長期化を司る分子メカニズムの解明を目指す。具体的には、神経活動に依存して翻訳される候補タンパク質を細胞種特異的に同定する。記憶の長期化に関わる実行因子の動態を細胞種特異的に可視化、操作する遺伝学的技術を確立し、タンパク質の発現レベルと細胞内局在の機能的意義を明らかにする。 今年度は、細胞腫特異的なリボソームプロファイリングの実験条件を最適化した。 具体的には、研究代表者らが開発したトランスジェニック系統を用いてエピトープタグを付したリボソームを特定の細胞に発現させる。RNase処理と免疫沈降法により翻訳中のmRNA断片を回収し、このmRNA断片を網羅的に解読することで、ゲノム配列に照らし合わせて翻訳活性を測定した。この手法を神経細胞およびグリア細胞に適用し、リボソームプロファイリングを行うことで、各遺伝子に対する翻訳活性の検出に成功した。

  8. 多機能を実現する細胞ネットワークの情報処理特性

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2020/04 - 2022/03

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    脳神経系は外界の情報を処理するために、外部入力に応じてその情報出力を可塑的に変化させる機能を持つ。ショウジョウバエのキノコ体という脳構造は、同一の匂い情報に対して、相反する行動を誘起する報酬・忌避記憶を形成する。この適応的な行動変化は、記憶の獲得・保持・読み出しの素過程に区別できるが、二つの情動記憶の全ての素過程はキノコ体で処理されることが知られている。 本研究では、キノコ体の神経細胞集団がどのように異なる状況に応じた入力情報を処理するのか、構造と機能の両面から明らかにする。相反する記憶の各素過程におけるキノコ体出力の状態変化の法則を解析し、キノコ体神経回路の情報処理特性の理解を目指す。 今年度は各出力神経を形態学的にプロファイルし、同一脳画像上に全ての出力神経をマップした「キノコ体出力回路図」を作成した。細胞の形態情報に加えて、各神経細胞の入出力部位の分布や放出される神経伝達物質などの情報を統合し、出力神経回路の構造的特徴を定量的に分類した。 さらに、出力神経回路図と情動記憶発現におけるキノコ体の出力パターン（機能コード）を解析することで、神経伝達物質と記憶の各素過程での機能の連関を検討した。その結果、一部の素過程に共通する神経回路モチーフを同定した。例えば、フィードバック回路を形成するグルタミン酸作動性のキノコ体出力神経が報酬記憶の獲得に重要な役割を担っていることを明らかにした。

  9. Drosophila acquires seconds-scale rhythmic behavior

    Tanimoto Hiromu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2019/06/28 - 2021/03/31

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    Detection of the temporal structure of stimuli is crucial for prediction. While perception of interval timing is relevant for immediate behavioral adaptations, it has scarcely been investigated, especially in invertebrates. Here, we examined whether the fruit fly, Drosophila melanogaster, can acquire rhythmic behavior in the range of seconds. To this end, we developed a novel temporal conditioning paradigm utilizing repeated electric shocks. Combined automatic behavioral annotation and time frequency analysis revealed that behavioral rhythms continued after cessation of the shocks. Furthermore, we found that aging impaired interval timing. This study thus not only demonstrates the ability of insects to acquire behavioral rhythms of a few seconds, but highlights a life-course decline of temporal coordination, which is also common in mammals.

  10. Understanding the diversity of neurotransmitter receptor expression in the fly brain through endogenous gene tagging

    Tanimoto Hiromu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

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    Neurotransmitters often have multiple receptors that induce distinct responses in receiving cells. Expression and localization of neurotransmitter receptors in individual neurons are therefore critical for understanding the operation of neural circuits. Here we describe a comprehensive library of reporter strains in which a convertible T2A-GAL4 cassette is inserted into endogenous neurotransmitter receptor genes of Drosophila. Using this library, we profile the expression of 75 neurotransmitter receptors in the brain. Cluster analysis reveals neurochemical segmentation of the brain, distinguishing higher brain centers from the rest.

  11. 記憶学習において作動する神経回路の遷移

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2017/06/30 - 2019/03/31

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    ショウジョウバエの匂い連合学習では、記憶の獲得・保持・読み出しの過程を明確に区別でき、その全ての過程がキノコ体と呼ばれる単一の脳構造で処理される。本研究では、記憶の素過程を定義するキノコ体出力神経の機能パターンと、その遷移を理解することを目的とする。研究代表者らの研究グループは、キノコ体の出力を担う21種類の出力神経を全て同定した。つまりキノコ体は、全ての神経種が遺伝学的に標識でき、機能操作と行動出力との因果関係を明らかにすることができる独自のモデル回路である。本研究ではこの遺伝学的リソースを用い、キノコ体の出力を構造と機能の両面から解析する。 2018年度は、キノコ体の異なる記憶の素過程における出力パターンを調べるために、嗅覚報酬・嗅覚罰記憶の各素過程における各出力神経の必要性を定量した。全ての出力神経について、その神経伝達を各素過程特異的に阻害し、記憶学習実験を行った。各出力神経の記憶に対する阻害効果から、各素過程での貢献度を定量することで、キノコ体からの出力コードが時間とともに変化する様子を明らかにした。さらに、各素過程の機能コードを実際の顕微鏡画像上にプロットし、その出力先、放出される神経伝達物質を可視化した。各出力神経細胞種の形態的特徴と記憶の各素過程の機能コードを統合することにより、例えば報酬記憶の獲得に共通する神経回路モチーフを同定した。

  12. Temporal conditioning in insects

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Tohoku University

    2016/04/01 - 2018/03/31

  13. Neural circuits that distinguish memory processes in the fly brain

    Tanimoto Hiromu, YAMAGATA Nobuhiro, ICHINOSE Toshiharu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2017/03/31

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    Fruit flies Drosophila melanogaster bidirectionally adapt the response to an odor based on appetitive and aversive memories of sugar reward and electric shock punishment, respectively. Accumulating evidence suggest that the output from a brain structure called the mushroom body (MB) is necessary for acquisition, consolidation, and retrieval of appetitive and aversive memories. However, it is largely unclear how the common MB circuit operates these distinct memory processes. To comprehensively characterize the organization of the MB output, we anatomically identified all the MB output neuron types in Drosophila. By measuring the behavioral importance of each output pathway, we found that the different sets of the MB output neurons are recruited in appetitive and aversive memories. Furthermore, our comprehensive behavioral analysis revealed acquisition, consolidation and retrieval of memories are characterized by the combinatorial functions of MB output neurons.

  14. Direct visualization of endogenous gene expression through targeted genetic labeling

    Tanimoto Hiromu, YAMAGATA Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    Comprehensive connectivity maps of nervous systems and connectomics are providing detailed designs of neuronal circuits. Although these connectivity maps are powerful in predicting hypothetical circuit functions and guiding new experiments thereon, the information of neurotransmitters and receptors is critical in interpreting how a given circuit functions. This project research aims at comprehensive mapping of neurotransmitters in the Drosophila brain. Using the CRISPR/Cas9 system, we generated a transgenic library of GAL4 knock-in that recapitulate the expression of genes for neurotransmitter biosynthesis and their receptors. By visualizing their expression patterns in the brain, we quantitatively compare the distribution of neurotransmitter-releasing and receiving cells. This study provided the basis of neurotransmitter map in the fly brain.

  15. 長期報酬記憶を制御するフィードバック神経回路

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2014/04/01 - 2016/03/31

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    短期記憶・長期記憶などに分類されるように、生理学的に異なったプロセスを経て形成される記憶の成分比は、時間経過に伴って変化する。特に長期記憶は学習後一定時間経ってから形成され始めるなど、その形成過程には謎が多い。これを構成する神経細胞がいつ、どのように働くかの解明は、神経回路の発現する機能を理解するうえで不可欠である。我々は先行研究で、短期報酬記憶と長期報酬記憶は独立して形成されることを示した。さらに、長期報酬記憶を特異的に誘導するドーパミン細胞種を同定した。このドーパミン神経細胞は、昆虫の連合記憶中枢であるキノコ体の特定領域に入力する。本研究では、この領域からの出力神経が、ドーパミン入力神経に再び入力するというフィードバック回路を形成しているという仮説に基づき、その形態の詳細な解析を行った。 平成27年度は記憶の形成、固定化など様々なタイミングで強制的にフィードバック回路の機能阻害または活性化を行い、各時点におけるその重要性を調査した。この結果、これに関わる神経回路に機能不全を持つハエは長期記憶を形成できないことが分かった。すなわち、報酬情報がキノコ体のフィードバック回路によって保持され、安定した長期記憶の形成に重要な役割を担うということが証明された。この成果は、Ichinose et al., eLife, 2015に代表される4編の原著論文として報告した。 <BR> <BR>

  16. 異なる感覚様式の記憶による行動制御と神経回路

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2014/04/01 - 2016/03/31

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    記憶には、色や音、匂い、味、手触りといった様々な感覚成分が一体となって結びついている。これらの感覚と紐付けられた記憶は、同じ報酬や罰によって形成されるにも関わらず、それぞれの強度や性質が全く異なる。記憶によって導かれる予測価値の差異が、どのような神経メカニズムによるのかの解明は、適応的行動の発現を理解するうえで重要である。本研究では、ショウジョウバエの砂糖報酬・電気ショック罰情報と感覚情報の統合の場である脳構造キノコ体に焦点を当て、色と匂いという2つの感覚情報が処理される神経回路の比較を通じて、このような予測価値の差異が生じる要因を探った。 平成27年度は、訓練時にキノコ体の標的細胞を単一細胞レベルで機能阻害することで、視覚・嗅覚記憶における報酬・罰回路を構成するドーパミン神経をそれぞれ同定し、これらの解剖学的、行動学的な比較を行った。この結果、報酬や罰を伝達する神経細胞の機能を妨げると、嗅覚と視覚の両者ともに、連合記憶の形成に支障が生じることが確認された。すなわち、色と匂いについては独立した記憶中枢で処理されるのではなく、両者とも同じ神経回路において一括で処理されるということが明らかとなった。この成果は、Vogt et al,. eLife, 2016に代表される4編の原著論文として報告した。

  17. 新しい嗅覚学習実験系の確立

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 研究活動スタート支援

    Category: 研究活動スタート支援

    Institution: 東北大学

    2013/08/30 - 2015/03/31

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    記憶学習を司る分子メカニズムを理解するためのモデルとして、ショウジョウバエの嗅覚連合学習系はこれまで盛んに研究されてきた。近年の遺伝学的技術の発展に伴い、複数の変異体の組み合わせや複雑な遺伝子型のハエの連合記憶を測定することが必要となるなど、行動実験の大規模化が進んでいる。 これらの行動学的解析には一般的に非常に時間がかかることに加え、これまでの研究により多数の記憶変異体が単離されてきたが、これら変異体の記憶読み出しにおける匂いの選択行動の詳細な解析はなされていない。本研究では、新たなシステムを構築し、実験作業・データ解析を自動化することで嗅覚学習実験系の高速化を実現した。また、学習に必要な刺激（匂い刺激、電気ショック刺激）の提示とデータ収集・解析をコンピュータプログラミングにより自動化することで実験を高速化するとともに、匂い選択行動の詳細な画像解析を行う実験装置を構築した。 本研究において、四グループのハエに対し同時に自動で嗅覚罰訓練を行う訓練装置、四グループのハエの嗅覚記憶を同時に自動で測定・解析するテスト装置を作製した。訓練装置では、学習に必要な電気ショック刺激、匂い刺激の提示をコンピュータからの指令により自動で制御する。テスト装置では、透明な台形チューブ内で匂いの選択をハエに行わせ、その選択行動を上部に配置したカメラにより撮影する。撮影された画像はコンピュータにおいて自動解析され、学習スコアが算出される。これらの訓練装置とテスト装置を二台ずつ並べて実験することで、８グループのハエの行動実験を自動で行うことができるようになった。また、これまでの研究手法では、記憶学習後の匂い選択行動は、それぞれの匂いを選択したハエの数を手作業でカウントするという方法しかとられていなかったが、本テスト装置では匂いの選択行動をカメラで撮影するため、その詳細な画像解析が可能となった。

  18. 2種類のモルフォゲンからの位置情報を統合する機構

    谷本 拓

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東京大学

    2000 - 2001

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Social Activities 7

  1. 世界脳週間2024 合同研究室ツアー

    サイエンスカフェ

    2025/03/20 - 2025/03/20

  2. Neural circuits tuning memory in the fly brain

    NCKU Department of Life Sciences International exchange seminar

    2024/03/12 - 2024/03/12

  3. 「動物らしさ」を研究する

    仙台第二高校「一日大学」

    2023/12/14 - 2023/12/14

  4. OLYMPUS Innovation Forum 2017 in Kobe

    2017/12/06 - 2017/12/06

  5. Dopamine circuits and memory formation

    RIKEN Brain Science Institute Summer Program

    2016/06/20 - 2016/06/20

  6. 脳が生みだす「好き嫌い」のしくみ

    市民のためのサイエンス講座2018「最新脳科学が解き明かす 脳とは何か？～脳を知って心を知る～」

    2018/12/09 -

  7. 第38回日本神経科学大会オリンパス株式会社共催セミナー

    2015/07/30 -

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    第38回日本神経科学大会において、オリンパス株式会社共催で「Mapping neural circuits for memory formation」と題したセミナーを行った。

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Media Coverage 9

  1. 東北大チーム、クラゲで確認 満腹→ホルモンが食欲抑制 摂食障害治療のヒントに？

    日経新聞

    2023/05

  2. 脳を持たないクラゲ 信号物質で食欲調節

    毎日新聞

    2023/04

    Type: Newspaper, magazine

  3. 脳はないけど…クラゲも「満腹感」 東北大グループ、食欲制御する神経物質発見

    河北新報

    2023/04

    Type: Newspaper, magazine

  4. 昆虫にも「楽天家」 東北大研究グループ、ハエの学習行動から解明

    河北新報

    2021/08

    Type: Newspaper, magazine

  5. ハエ老化 リズム感低下

    河北新報

    2021/04

    Type: Newspaper, magazine

  6. ハエで知る脳の基本原理

    読売新聞

    2018/11/25

    Type: Newspaper, magazine

  7. 脳科学から心知る

    読売新聞

    2018/11/09

    Type: Newspaper, magazine

  8. artificial memories created in drosophila, by simulating sugar reward using direct activation of dopamine Mb’sneurons.

    Arte TELEVISION, France & Germany

    2016/10

    Type: TV or radio program

  9. Fragrant Flashbacks 記憶を立ちのぼらせる香りの力

    T JAPAN: The New York Times Style Magazine

    2015/05/25

    Type: Newspaper, magazine

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Other 4

  1. ショウジョウバエ脳の神経ペプチドサーキットの再構築

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    神経ペプチドは、生存に重要な動物行動を制御する。特にショウジョウバエでは、神経ペプチドによるドーパミン系の制御が、学習や摂食行動をコントロールしている。本研究では、神経ペプチドの網羅的な形態解析を行う。PC 上での画像重ね合わせを駆使し、in silico での神経ペプチドの脳サーキット構築・分析を行うことで、ハエ脳における神経ペプチドデータバンクの作成を目指す。

  2. 異なる感覚モダリティによる記憶の脳内表現

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    現象の認知は、対象の情報を視覚、嗅覚、聴覚、など様々な感覚器官から得て、それらを脳で統合することで成立する。これらを統合する脳部位には、異なる感覚モダリティ情報を同時に処理する機能が備わっていなければならない。本研究では、色と匂いの情報が、昆虫の記憶中枢においてどのように表現されているのか、単一神経種レベル、あるいは細胞レベルで解明することを目的とする。また、視覚野からキノコ体への情報伝達回路を明らかにすることにより、記憶中枢が異なる感覚モダリティ情報を処理する神経回路メカニズムを理解する。

  3. 文部科学省・脳科学研究戦略推進プログラム課題G「脳科学研究を支える体系的・集約的な情報基盤の構築」

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    ショウジョウバエ成虫脳では、モノアミン神経群は樹状突起を脳の下部に持ち、脳の上部の広範な部位に投射するという、哺乳類脳と類似した構造を持っている。昆虫でも情動の制御にモノアミン神経経路が重要な役割を果たすことは確実視されているが、(1) 罰や報酬などの情報はどのようにして異なるモノアミン系の活動に結びつくのか？ (2) 異なるモノアミン系が活性化するとき、脳の中ではどのように異なった反応が起きているのか？という２つの重要な問題は、まだほとんど解析されていない。そこで前者を知るために、モノアミン神経がどのような神経から情報を受けるのかの回路ネットワークを明らかにし、それらの神経を人為的に遮断したり活性化したりすると脳機能にどのような変化が起こるかを解析する。また後者を知るために、異なるモノアミン系が活性化した脳で、生化学的にどのような変化が起きているかを解析する。これによって神経回 路と神経細胞内という２つのレベルにまたがる多階層の解析を行う。

  4. モノアミン作動性神経の選択的機能阻害

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    本研究では、モノアミン産生細胞のみを選択的に阻害する外来遺伝子をショウジョウバエに導入し、自由行動下でのモノアミン作動性神経の機能を操作する実験系を確立する。具体的には、モノアミンを特異的に化学修飾する外来遺伝子（ebony,DβH）を発現させ、当該神経の不活性化を試みる。