Details of the Researcher

PHOTO

Takahiro Ito
Section
Graduate School of Medicine
Job title
Senior Assistant Professor
Degree

  • (理学) (Yamagata University)

Profile

2005年3月 山形大学大学院理工学研究科博士後期課程 卒業
2005年4月 東北大学先進医工学研究科阿部研究室 技術補佐員
2008年1月 東北大学先進医工学研究科阿部研究室 博士研究員
2008年4月 山形大学大学院理工学研究科阿部研究室 博士研究員
2008年9月 クリノ株式会社 テクニカルアドバイザー
2012年3月 東北大学原子分子材料科学高等研究機構末永研究室 研究員
2012年6月 東北大学未来科学技術共同研究センター後藤研究室 技術補佐員
2013年12月 東北大学大学院医工学研究科川瀬研究室 特任助教
2015年12月 東北大学原子分子材料科学高等研究機構末永研究室 研究員
2016年4月 東北大学大学院環境科学研究科末永研究室 研究員

2023年4月 山梨大学大学院総合研究部工学域基礎教育センター井上研究室 研究助教

2023年10月 東北大学大学院医工学研究科 永富研究室 学術研究員

2024年4月 東北大学産学連携機構イノベーション戦略推進センター未来社会健康デザイン拠点 学術研究員

2025年4月 東北大学大学院医学系研究科運動学分野山田研究室 講師

Research History 14

  • 2025/04 - Present
    Tohoku University

  • 2024/04 - 2025/03
    Tohoku University　Head Office of Enterprise Partnerships

  • 2023/10 - 2024/03
    Tohoku University

  • 2023/04 - 2023/09
    山梨大学大学院　総合研究部工学域基礎教育センター　研究助教

  • 2022/04 - 2023/03
    Tohoku University　Graduate School of Biomedical Engineering

  • 2018/04 - 2022/03
    東北大学大学院　環境科学研究科　学術研究員

  • 2015/12 - 2018/03
    Tohoku University

  • 2013/12 - 2015/11
    東北大学大学院　医工学研究科　特任助教

  • 2012/06 - 2013/11
    Tohoku University　New Industry Creation Hatchery Center

  • 2012/03 - 2012/05
    Tohoku University

  • 2008/09 - 2012/02
    クリノ株式会社　テクニカルアドバイザー

  • 2008/04 - 2008/08
    Yamagata University　Graduate School of Science and Engineering

  • 2008/01 - 2008/03
    Tohoku University　Biomedical Engineering Research Organization

  • 2005/04 - 2007/12
    Tohoku University　Biomedical Engineering Research Organization

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Education 3

  • Yamagata University　Graduate School of Science and Engineering

    2002/04 - 2005/03

  • Yamagata University　Graduate School of Science and Engineering

    2000/04 - 2002/03

  • Yamagata University　Faculty of Science　Department of Biology

    1996/04 - 2000/03

Research Interests 3

  • sport and health science

  • reproductive biology

  • Medical and Biological Engineering

Research Areas 1

  • Life sciences / Medical systems /

Papers 28

  1. Au paste electrode to increase the intensity of cathodic electrochemiluminescence Peer-reviewed

    Ayane Endo, Koki Kubota, Takahiro Ito-sasaki, Kumi Y. Inoue

    ECS Meeting Abstracts　MA2024-02　(67)　4765-4765　2024/11/22

    Publisher: The Electrochemical Society

    DOI： 10.1149/ma2024-02674765mtgabs 　

    eISSN： 2151-2043

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    Bipolar electrochemical microscopy (BEM) is a novel electrochemical imaging platform using a vertical closed bipolar electrode (cBPE) array as an imaging element. In BEM, the electrochemical reaction generated at one end of the BPE is converted to electrochemiluminescence (ECL) at the other end of the BPE. Unlike the conventional electrode array method, which requires lead wires for each electrode, BEM can operate without wires to each electrode. Therefore, imaging with both high-temporal and high-spatial resolution is possible, from which advantage, BEM is expected to be a tool for the analysis of biological phenomena that occur in a very short period in a local area on the scale of a single cell. As a simple method for fabricating vertical cBPE arrays, Iwama et al. previously proposed a method of filling conducting carbon pastes (CPs) into pores of a glass capillary plate.1 As another direction of BEM development research, we are conducting dopamine (DA) detection using N,N’-dimethyl-3,4,9,10-perylenetetracarboxylicdiimide (PDI-CH3) as a cathodic luminophore for ECL.2 We are currently investigating the use of a PDI-CH3 mixed paste electrodes as BEM detection elements for DA imaging, taking advantage of the flexibility and ease of modification of the paste electrode. In the process, we found a phenomenon in which ECL is enhanced using Au paste (AuP).3 In this study, we compared PDI-CH3 mixed CP and AuP electrode by three-electrode system and cBPE-ECL detection system. CP was prepared by mixing acetylene carbon black (particle size 42 nm), and liquid paraffin at a weight ratio of 1:1. AuRoFUSE (particle size 0.2–0.4 µm) was used for AuP. The PDI-CPs were obtained by wet mixing of PDI-CH3 and CP at weight ratios from 4:1 to 1:20 in an alumina mortar with ethanol. PDI-AuPs were obtained in a similar way by wet mixing of PDI-CH3 and AuP at weight ratios from 1:5 to 1:20. Each paste was filled into a paste electrode holder (1.6 mm in diameter). PDI-CH3/CP 1:1 by weight and PDI-CH3/AuP 1:20 by weight contain the same volume of PDI-CH3 in a given volume of each paste, because the specific gravities of CP and AuP are speculated 0.95 g/cm3 and 19.3 g/cm3, respectively. Measurements using three-electrode system was conducted with 35 mm dish filled with 0.2 M Na2S2O8 (containing phosphate-buffered saline; PBS), a working electrode (WE; 1.6 mm in diameter), a counter electrode (CE; platinum plate), and a reference electrode (RE; Ag/AgCl). Linear sweep voltammograms (LSVs) and ECL intensities were obtained at a scan rate of 50 mV/s with 500 ms exposure time of a CMOS camera. In the cBPE-ECL detection system, two 35 mm dishes filled with 0.2 M Na2S2O8 (containing PBS) and 0 or 1 mM DA (containing PBS) were used as a detection cell and a sample cell, respectively. A paste electrode to be evaluated in the detection cell and a glassy carbon electrode (1.6 mm in diameter) in the sample cell were connected to each other to be a cBPE. As driving electrodes, a platinum plate in the detection cell, an Ag/AgCl electrode and a second platinum plate in the sample cell were connected to the WE, RE, and CE terminals of a potentiostat, respectively. LSVs and ECL intensities were obtained at a scan rate of 20 mV/s with 5 s exposure time of the CMOS camera. At the three-electrode system, LSVs of PDI-CH3/CP 1:1 and PDI-CH3/AuP 1:20 in weight ratio, containing the same volumes of PDI-CH3 in the pastes, produced similar maximum values of the first current peak related to ECL reaction, while the peak ECL intensities were 1.75 times higher in case using PDI-AuP than in case using PDI-CP. This result suggests that the ECL intensity can be enhanced using AuP. This ECL enhancement may be due to the surface plasmon field-enhancement effect by Au particles on the electrode surface, given that the AuRoFUSE particle size is 0.2 to 0.4 µm. The figure shows the images obtained by CMOS camera at 1.2 V using cBPE-ECL detection system. The ECL intensities peaks originating from DA oxidation in the sample cell were 9.5 times higher using PDI-AuP rather than PDI-CP, even though the peak current values obtained using PDI-AuP and PDI-CP were almost the same. By using AuP instead of CP, imaging such as DA is expected be with high sensitivity in BEM system. References: T. Iwama et al., Anal. Chem., 94, 8857 (2022). T. Iwama et al., available at SSRN, http://dx.doi.org/10.2139/ssrn.4632693. A. Endo et al., Electrochemistry, 92, 022013 (2024). Figure 1 <p></p>

  2. (Invited) Development of Bipolar Electrochemical Microscopy for High-Speed and High-Resolution Monitoring of Biochemical Phenomena

    Kumi Y. Inoue, Koki Kubota, Ayane Endo, Tomoki Iwama, Takahiro Ito-Sasaki, Hitoshi Shiku

    ECS Meeting Abstracts　MA2024-02　(65)　4343-4343　2024/11/22

    Publisher: The Electrochemical Society

    DOI： 10.1149/ma2024-02654343mtgabs 　

    eISSN： 2151-2043

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    Closed bipolar electrochemistry (cBPE) involves simultaneous oxidation and reduction at opposite poles of a conductor in two separated solutions. (Figure left). The unique property of cBPE to be operated without direct connection to an external power source is expected to lead to higher resolution in electrochemical imaging than the conventional microelectrode array type imaging platforms. We have applied cBPE to an electrochemical microscopy (BEM) (Figure right) for high-resolution biochemical imaging.1- 7 The operation principle of BEM has been successfully verified,1 then the current focus is on fabrication method of cBPE array. Previously, we filled Au into pores of a polyethylene terephthalate track-etched membrane having D=8 µm pores by electroless plating using 50 mM HAuCl4 aqueous solution and 50 mM NaBH4 EtOH solution.2 Using this cBPE array, an image of oxygen concentration distribution around cell spheroids was successfully obtained, but this method has a problem of the inability to completely fill the pores with gold, resulting in large ionic currents. In this study, we use conductive pastes for fabrication of vertical closed bipolar electrode array to completely fill the pores with conductive materials. Epoxy-based carbon paste (ECP) was prepared by mixing acetylene carbon black (ACB; particle size=42 nm; Strem Chemicals) and epoxy resin (mixture of Epok 812 (62 mL), DDSA (100 mL), and DMP-30 (1.5%); Okenshoji). Oil-based carbon paste (OCP) was prepared by mixing the ACB and the liquid paraffin. For Au paste (AuP), commercially available AURoFUSE (particle size (D50) 0.2–0.4 µm; Tanaka Kikinzoku Kogyo) was used as is. Vertical cBPE arrays using the carbon paste filled in pores of a glass capillary plate was fabricated by filling ECP (ACB: epoxy resin=1:4 in weight ratio) cured at 60°C for 15 h. After cooling, the surface was coated by OCP (ACB: liquid paraffin=1:1 in weight ratio) to fill the dented parts shrank by heat curing. For [Fe(CN)6]3 − imaging, a sample side of the array was modified with reduced graphene oxide (RGO) by coating OCP containing 5 wt.% RGO (RGO-OCP). For H2O2 imaging, a sample side of the array was modified with Prussian blue (PB) by coating commercially available PB containing carbon paste (Carbon Mediator Paste, C2070424P2; SunChemical Gwent). The BEM system was constructed using two solution chambers (detection/sample chambers), BPE array, and driving electrodes. The detection chamber located at the bottom side of the BPE array was filled with 10 mM [Ru(bpy)3] Cl2/20 mM tripropylamine (TPA) solution containing phosphate buffered saline (PBS). The sample chamber located at the bottom side was filled with the sample solution for each measurement. A platinum driving electrode inserted into the detection chamber, another platinum driving electrode and a Ag/AgCl reference electrode inserted into the sample chamber were connected WE, CE and RE connector of a potentiostat, respectively. The entire system was mounted on an inverted microscope with a high sensitivity CCD camera to observe the electrochemiluminescence (ECL) generated at the underside of the BPEs. By using RGO and PB modified carbon paste (PC) cBPE array for BEM element, we successfully obtained an image of 5 mM [Fe(CN)6]3− and H2O2 inflow, respectively.5 These results show that cBPE arrays for BEM can be fabricated by filling micropores with carbon paste taking advantage of the flexibility and ease of modification of the paste electrode. We are now investigating the use of AuP as an electrode material of cBPE array. We have obtained the result that ECL can be enhanced by using AuP as an electrode material.7 This may be due to the surface plasmon enhancement effect by the Au particles contained in the AuP. The establishment of a simple method for fabricating vertical cBPE arrays without ion leakage is expected to further accelerate the research and development of BEM. References: Iwama, K. Y. Inoue, H. Abe, T. Matsue, Chem. Lett., 47, 843–845 (2018) Iwama, K. Y. Inoue, H. Abe, T. Matsue, H. Shiku, Analyst, 145, 6895–6900 (2020) Iwama, M. Komatsu, K. Y. Inoue, H. Shiku, ChemElectroChem, 8, 3492–3498 (2021) Iwama, Y. Guo, S. Handa, K. Y. Inoue, T. Yoshinobu, F. Sorin, H. Shiku, Adv. Mater. Technol., 7, 2101066–2101066 (2022) Iwama, K. Y. Inoue, H. Shiku, Anal. Chem., 94, 8857–8866 (2022) Iwama, M. Komatsu, K. Y. Inoue, K. Kubota, T. Ito-Sasaki, H. Shiku, SSRN, posted 28 Nov 2023, available at https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4632693 A. Endo, K. Kubota, T. Ito-Sasaki, M. Komatsu, T. Iwama, H. Shiku, K. Y. Inoue, Electrochemistry, 92, 022013 (2024). Figure 1 <p></p>

  3. Bipolar electrochemical sensor with perylene diimide-based cathodic luminophore for dopamine detection and imaging. International-journal Peer-reviewed

    Tomoki Iwama, Mayo Komatsu, Kumi Y Inoue, Koki Kubota, Takahiro Ito-Sasaki, Hitoshi Shiku

    Talanta　278　126509-126509　2024/10/01

    DOI： 10.1016/j.talanta.2024.126509 　

    More details Close

    Bipolar electrochemical microscopy (BEM), which visualizes the concentration distribution of molecular species in biological systems by electrochemiluminescence (ECL), is expected to be applied to the high-spatiotemporal-resolution imaging of biomolecules, enabling the analysis of cellular functions. In the past, the molecular species that could be imaged by BEM were generally restricted to oxidized molecules due to the limitation derived from the ECL mechanism of the luminophore. Recently, the imaging of dopamine (DA), a reduced molecule, was achieved using Ru (bpy)32+/glutathione disulfide (GSSG) as a cathodic luminophore. However, a large driving voltage was required for ECL generation, resulting in a low S/N ratio. In this study, we employed N,N'-dimethyl-3,4,9,10-perylenetetracarboxylic diimide (PDI-CH3)/potassium peroxodisulfate (K2S2O8), which is a cathodic luminophore that can be reduced at a nobler potential to produce ECL than [Ru(bpy)3]2+/GSSG. First, the ECL mechanism of PDI-CH3/K2S2O8 was elucidated by using a PDI-CH3 drop-cast glassy carbon electrode (GCE) immersed in K2S2O8 solution as the working electrode in a 3-electrode system. The PDI-CH3 drop-casted GCE, a single closed bipolar electrode (c-BPE), was used as the cathode in the successful quantification of 50-500 μmol L-1 DA in a sample chamber in which a c-BPE anode was immersed, resulting in a high S/N. The selective detection of DA in the presence of ascorbic acid was achieved by modifying the anode with Nafion. Finally, DA imaging was demonstrated using a commercially available anisotropic conducting film with PDI-CH3 coating on the cathode surface as a c-BPE array. The change in the concentration distribution in the inflow of DA was successfully imaged based on the change in the ECL intensity at the c-BPE cathode. This BEM system is expected to be useful for DA imaging of the brain.

  4. Relationship between Plasma Lipopolysaccharide Concentration and Health Status in Healthy Subjects and Patients with Abnormal Glucose Metabolism in Japan: A Preliminary Cross-Sectional Study Peer-reviewed

    Nobuo Fuke, Shojiro Sawada, Takahiro Ito-Sasaki, Kumi Y. Inoue, Yusuke Ushida, Ikuo Sato, Tomokazu Matsue, Hideki Katagiri, Hiroyuki Ueda, Hiroyuki Suganuma

    J　6　(4)　605-626　2023/11/30

    Publisher: MDPI AG

    DOI： 10.3390/j6040040 　

    eISSN： 2571-8800

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    Lipopolysaccharides are components of Gram-negative bacteria. The relationship between blood lipopolysaccharide levels and health status has mainly been investigated in Europe, and there is a lack of information about Asia, particularly Japan. This study aimed to investigate the relationship between blood lipopolysaccharide levels and health status in the Japanese. We conducted two cross-sectional studies in 36 healthy subjects (Study 1) and 36 patients with abnormal glucose metabolism (AGM; Study 2). The plasma lipopolysaccharide concentration in healthy subjects was positively correlated with body mass index. The plasma lipopolysaccharide concentration in AGM patients was obviously higher than that in healthy subjects. Furthermore, in AGM patients, the plasma lipopolysaccharide concentration was positively correlated with C-peptide, fasting plasma glucose levels, triglycerides, and stage of diabetic nephropathy. The plasma lipopolysaccharide concentration was also negatively correlated with 20/(C-peptide × fasting plasma glucose), an indicator of insulin resistance, and high-density lipoprotein cholesterol. In particular, the correlation between plasma lipopolysaccharide concentration and triglycerides in AGM patients was maintained in multiple regression analyses adjusted for age, sex, or body mass index. These results suggest a possible role of lipopolysaccharides in obesity in healthy subjects and in the deterioration of triglyceride metabolism in AGM patients in the Japanese population.

  5. Highly Sensitive Electrochemical Endotoxin Sensor Based on Redox Cycling Using an Interdigitated Array Electrode Device. International-journal Peer-reviewed

    Kentaro Ito, Kumi Y Inoue, Takahiro Ito-Sasaki, Miho Ikegawa, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku

    Micromachines　14　(2)　2023/01/27

    DOI： 10.3390/mi14020327 　

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    The Limulus amebocyte lysate (LAL) reaction-based assay, the most commonly used endotoxin detection method, requires a skilled technician. In this study, to develop an easy-to-use and highly sensitive endotoxin sensor, we created an electrochemical endotoxin sensor by using an interdigitated array electrode (IDAE) device with advantages of amplifiable signals via redox cycling and portability. We added Boc-Leu-Gly-Arg-p-aminophenol (LGR-pAP) as an electrochemical substrate for an LAL reaction and detected p-aminophenol (pAP) released from LGR-pAP as a product of an endotoxin-induced LAL reaction via an IDAE device. The IDAE device showed a great redox cycling efficiency of 79.8%, and a 4.79-fold signal amplification rate. Then, we confirmed that pAP was detectable in the presence of LGR-pAP through chronoamperometry with the potential of the anode stepped from -0.3 to 0.5 V vs. Ag/AgCl while the cathode was biased at -0.3 V vs. Ag/AgCl. Then, we performed an endotoxin assay by using the IDAE device. Our endotoxin sensor detected as low as 0.7 and 1.0 endotoxin unit/L after the LAL reaction for 1 h and 45 min, respectively, and these data were within the cut-off value for ultrapure dialysis fluid. Therefore, our highly sensitive endotoxin sensor is useful for ensuring medical safety.

  6. Enhancement of Electrochemiluminescence by Au Paste Electrode for Bipolar Electroanalysis Peer-reviewed

    Ayane ENDO, Koki KUBOTA, Takahiro ITO-SASAKI, Mayo KOMATSU, Tomoki IWAMA, Hitoshi SHIKU, Kumi Y. INOUE

    Electrochemistry　2023

    Publisher: The Electrochemical Society of Japan

    DOI： 10.5796/electrochemistry.23-68114 　

    ISSN： 1344-3542

    eISSN： 2186-2451

  7. Bipolar Electrochemical Sensor with Perylene Diimide-Based Cathodic Luminophore for Dopamine Detection and Imaging International-journal Peer-reviewed

    Kumi Y. Inoue, Tomoki Iwama, Mayo Komatsu, Koki Kubota, Takahiro Ito-Sasaki, Hitoshi Shiku

    Talanta　278　126509-126509　2023

    Publisher: Elsevier BV

    DOI： 10.2139/ssrn.4632693 　

    More details Close

    Bipolar electrochemical microscopy (BEM), which visualizes the concentration distribution of molecular species in biological systems by electrochemiluminescence (ECL), is expected to be applied to the high-spatiotemporal-resolution imaging of biomolecules, enabling the analysis of cellular functions. In the past, the molecular species that could be imaged by BEM were generally restricted to oxidized molecules due to the limitation derived from the ECL mechanism of the luminophore. Recently, the imaging of dopamine (DA), a reduced molecule, was achieved using Ru (bpy)32+/glutathione disulfide (GSSG) as a cathodic luminophore. However, a large driving voltage was required for ECL generation, resulting in a low S/N ratio. In this study, we employed N,N'-dimethyl-3,4,9,10-perylenetetracarboxylic diimide (PDI-CH3)/potassium peroxodisulfate (K2S2O8), which is a cathodic luminophore that can be reduced at a nobler potential to produce ECL than [Ru(bpy)3]2+/GSSG. First, the ECL mechanism of PDI-CH3/K2S2O8 was elucidated by using a PDI-CH3 drop-cast glassy carbon electrode (GCE) immersed in K2S2O8 solution as the working electrode in a 3-electrode system. The PDI-CH3 drop-casted GCE, a single closed bipolar electrode (c-BPE), was used as the cathode in the successful quantification of 50-500 μmol L-1 DA in a sample chamber in which a c-BPE anode was immersed, resulting in a high S/N. The selective detection of DA in the presence of ascorbic acid was achieved by modifying the anode with Nafion. Finally, DA imaging was demonstrated using a commercially available anisotropic conducting film with PDI-CH3 coating on the cathode surface as a c-BPE array. The change in the concentration distribution in the inflow of DA was successfully imaged based on the change in the ECL intensity at the c-BPE cathode. This BEM system is expected to be useful for DA imaging of the brain.

  8. Electrochemical Immunoassay with Dual-Signal Amplification for Redox Cycling within a Nanoscale Gap Peer-reviewed

    Kentaro Ito, Kumi Y. Inoue, Takahiro Ito-Sasaki, Kosuke Ino, Hitoshi Shiku

    ACS Applied Nano Materials　2021/10/22

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsanm.1c02837 　

    ISSN： 2574-0970

    eISSN： 2574-0970

  9. A Nanopore-based Endotoxin Sensor Peer-reviewed

    Kentaro ITO, Kumi Y. INOUE, Takahiro ITO-SASAKI, Tomokazu MATSUE, Hitoshi SHIKU

    Bunseki Kagaku　68　(8)　575--580　2019/08

    DOI： 10.2116/bunsekikagaku.68.575 　

    ISSN： 0525-1931

  10. APTAMER-BASED ALLERGEN SENSING SYSTEM FOR FOOD SAFETY

    Hamza Abdelli, Takashiro Tsukamoto, Takahiro Ito, Kumi Y. Inoue, Tomokazu Matsue, Shuji Tanaka

    2019 20TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS & EUROSENSORS XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)　932-935　2019

  11. Front Cover: Simultaneous Multiplex Potentiostatic Electroanalysis with Liquid-Junction-Removed Reference Electrode System using a Closed Bipolar Electrode (ChemElectroChem 16/2018) Peer-reviewed

    Kumi Y. Inoue, Miho Ikegawa, Takahiro Ito-Sasaki, Shinichiro Takano, Hitoshi Shiku, Tomokazu Matsue

    ChemElectroChem　5　(16)　2157-2157　2018/08/09

    Publisher: Wiley

    DOI： 10.1002/celc.201800895 　

  12. Development of novel detection system using bipolar electrode. Peer-reviewed

    Miho Ikegawa, Shinichiro Takano, Kumi Y. Inoue, Takahiro S. Ito, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

    Chemical Sensor　32　(Supplement A)　78-80　2016

  13. 着床能力の高い胚の選別を目的としたヒト凍結融解胚盤胞における酸素消費量特性の検討 Peer-reviewed

    前沢 忠志, 山中 昌哉, 橋本 周, 伊藤 隆広, 福田 愛作, 森本 義晴

    日本生殖医学会雑誌　58　(4)　286-286　2013/10

    Publisher: (一社)日本生殖医学会

    ISSN： 1881-0098

  14. 安価な簡易型センサの実用化に向けた電気化学エンドトキシン検出法の開発 Peer-reviewed

    井上(安田)久美, 高野 真一朗, 高橋 里子, 石田 洋祐, 伊藤(佐々木)隆広, 伊野浩介, 珠玖 仁, 末永 智一

    エンドトキシン・自然免疫研究　16　7-11　2013

  15. Monitoring oxygen consumption of single mouse embryos using an integrated electrochemical microdevice Peer-reviewed

    Yasumoto Date, Shinichiro Takano, Hitoshi Shiku, Kosuke Ino, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Tomokazu Matsue

    BIOSENSORS & BIOELECTRONICS　30　(1)　100-106　2011/12

    DOI： 10.1016/j.bios.2011.08.037 　

    ISSN： 0956-5663

  16. Developmental assessment of human vitrified-warmed blastocysts based on oxygen consumption Peer-reviewed

    Masaya Yamanaka, Shu Hashimoto, Ami Amo, Takahiro Ito-Sasaki, Hiroyuki Abe, Yoshiharu Morimoto

    HUMAN REPRODUCTION　26　(12)　3366-3371　2011/12

    DOI： 10.1093/humrep/der324 　

    ISSN： 0268-1161

  17. ヒト凍結融解胚盤胞の呼吸量測定 Peer-reviewed

    山中昌哉, 橋本周, 天羽杏実, 伊藤-佐々木隆広, 阿部宏之, 森本善晴

    産婦人科の実際　60　(6)　923-927　2011

    Publisher: 金原出版

    ISSN： 0558-4728

  18. A microfluidic dual capillary probe to collect messenger RNA from adherent cells and spheroids Peer-reviewed

    Hitoshi Shiku, Takeshi Yamakawa, Yuji Nashimoto, Yasufumi Takahashi, Yu-suke Torisawa, Tomoyuki Yasukawa, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Hideki Kambara, Tomokazu Matsue

    ANALYTICAL BIOCHEMISTRY　385　(1)　138-142　2009/02

    DOI： 10.1016/j.ab.2008.10.039 　

    ISSN： 0003-2697

  19. A Novel Predictive Method for Assessing the Quality of Isolated Pancreatic Islets Using Scanning Electrochemical Microscopy Peer-reviewed

    M. Goto, H. Abe, T. Ito-Sasaki, M. Goto, A. Inagaki, N. Ogawa, K. Fujimori, Y. Kurokawa, T. Matsue, S. Satomi

    TRANSPLANTATION PROCEEDINGS　41　(1)　311-313　2009/01

    DOI： 10.1016/j.transproceed.2008.10.075 　

    ISSN： 0041-1345

  20. Evaluating the Quality of Human Embryos with a Measurement of Oxygen Consumption by Scanning Electrochemical Microscopy

    UTSUNOMIYA Takafumi, GOTO Kaori, NASU Megumi, KUMASAKO Yoko, ARAKI Yasuhisa, YOKOO Masaki, ITOH SASAKI Takahiro, ABE Hiroyuki

    Journal of Mammalian Ova Research　25　(1)　2-7　2008/04/01

    Publisher: JAPANESE SOCIETY OF OVA RESEARCH

    DOI： 10.1274/jmor.25.2 　

    ISSN： 1341-7738

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    Morphological evaluation has been widely used to evaluate embryo quality because it is non-invasive and useful in predicting pregnancy rate. However, morphological evaluations are subjective and categorization standards often vary between investigators. The respiration rate of embryos is a useful parameter for evaluating embryo quality. The scanning electrochemical microscopy (SECM) measuring system provides a non-invasive, simple, accurate, and consistent measurement of the respiration activity of human embryos. After morphological evaluation by Veeck's method, oxygen consumption by individual human embryos was quantified by SECM. Fundamentally, the maturation of mitochondria correlated with an increase in oxygen consumption during the development of embryos. The development of mitochondria may be an important factor in embryo quality, because mitochondria provide ATP for embryonic development by metabolism of nutrients in the cytoplasm. The respiration rates on the day 3 after in vitro fertilization (IVF) were measured and significant differences in oxygen consumption were registered even among embryos with the same morphological classification. There were no significant differences between the mean rates of oxygen consumption at each cleavage stage, however, there was considerable variation in respiration rate within embryos of the same morphological grade. The safety of SECM is assured as the embryos which were examined by SECM for oxygen consumption showed the same development levels as the control group. These results support the hypothesis that measuring embryonic respiration provides additional and valuable information about embryo quality.<br>

  21. Multiple Analysis of Respiratory Activity in the Identical Oocytes by Applying Scanning Electrochemical Microscopy Peer-reviewed

    Masaki Yokoo, Takahiro Ito-Sasaki, Hitoshi Shiku, Tomokazu Matsue, Hiroyuki Abe

    TRANSACTIONS OF THE MATERIALS RESEARCH SOCIETY OF JAPAN, VOL 33, NO 3　33　(3)　763-766　2008

  22. Measurement of gene expression from single adherent cells and spheroids collected using fast electrical lysis Peer-reviewed

    Yuji Nashimoto, Yasufumi Takahashi, Takeshi Yamakawa, Yu-Suke Torisawa, Tomoyuki Yasukawa, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Hitoshi Shiku, Hideki Kambara, Tomokazu Matsue

    ANALYTICAL CHEMISTRY　79　(17)　6823-6830　2007/09

    DOI： 10.1021/ac071050q 　

    ISSN： 0003-2700

    eISSN： 1520-6882

  23. Oxygen consumption of mammalian embryos and Oocytes monitored by scanning electrochemical microscopy Peer-reviewed

    Hitoshi Shiku, Tomoyuki Yasukawa, Tornokazu Matsue, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe

    2007 IEEE SENSORS, VOLS 1-3　1408-1411　2007

    ISSN： 1930-0395

  24. Dual - Capillary micro - Fluidic probe to collect mRNA from a single adherent cells Peer-reviewed

    H. Shiku, T. Yamakawa, Y. Nashimoto, Y. Takahashi, Y. Torisawa, T. Yasukawa, T. Ito-Sasaki, M. Yokoo, H. Abe, H. Kambara, T. Matsue

    PROGRESS ON POST-GENOME TECHNOLOGIES　61-64　2007

  25. Estimation of respiratory activity of mouse embryos by scanning electrochemical microscopy Peer-reviewed

    Takahiro Ito-Sasaki, Naoto Okazaki, Masaki Yokoo, Hitoshi Shiku, Tomoyuki Yasukawa, Tomokazu Matsue, Hiroyuki Abe

    ZOOLOGICAL SCIENCE　23　(12)　1185-1185　2006/12

    ISSN： 0289-0003

  26. Microsystem surveying three dimensional (3D) culture and embryo development Peer-reviewed

    H. Shiku, Y. Nashimoto, Y. Torisawa, T. Yasukawa, T. Saito, T. Ito-Sasaki, M. Yokoo, H. Abe, T. Matsue

    Progress on Post-Genome Technologies　221-223　2006

  27. Oxygen consumption of cell suspension in a poly(dimethylsiloxane) (PDMS) microchannel estimated by scanning electrochemical microscopy Peer-reviewed

    Takeshi Saito, Ching-Chou Wu, Hitoshi Shiku, Tomoyuki Yasukawa, Masaki Yokoo, Takashi Ito-Sasaki, Hiroyuki Abe, Hiroyoshi Hoshi, Tomokazu Matsue

    ANALYST　131　(9)　1006-1011　2006

    DOI： 10.1039/b600080k 　

    ISSN： 0003-2654

  28. Ordered progress of spermiogenesis to the fertilizable sperm of the medaka fish, Oryzias latipes, in cell culture Peer-reviewed

    T Sasaki, A Watanabe, E Takayama-Watanabe, M Suzuki, H Abe, K Onitake

    DEVELOPMENT GROWTH & DIFFERENTIATION　47　(2)　87-97　2005/02

    DOI： 10.1111/j.1440-169x.2005.00785.x 　

    ISSN： 0012-1592

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Misc. 15

  1. クローズドバイポーラ電気化学顕微鏡の実現に向けた多孔質膜への導電性ペースト充填の検討—FILLING METHOD OF CONDUCTIVE PASTE INTO POROUS MEMBRANE FOR CLOSED BIPOLAR ELECTROCHEMICAL MICROSCOPE

    井上(安田) 久美, 遠藤 彩音, 久保田 恒喜, 水庫 詩苑, 伊藤-佐々木 隆広, 戸塚 友理

    Proceedings of the Chemical Sensor Symposium　73　88-90　2023/09

    Publisher: 電気化学会化学センサ研究会

  2. 世界初のLAL試薬を使用しないエンドトキシン検出法の開発

    伊藤-佐々木 隆広, 伊藤 健太郎

    バイオインダストリー　39　(1)　24-30　2022

  3. Simultaneous Multiplex Potentiostatic Electroanalysis with Liquid-Junction-Removed Reference Electrode System using a Closed Bipolar Electrode

    Kumi Y. Inoue, Miho Ikegawa, Takahiro Ito-Sasaki, Shinichiro Takano, Hitoshi Shiku, Tomokazu Matsue

    CHEMELECTROCHEM　5　(16)　2161-2161　2018/08

    DOI： 10.1002/celc.201800894 　

    ISSN： 2196-0216

  4. Close型バイポーラ電極による液絡不要の参照電極集積型アンペロメトリックセンシングプローブの開発.

    井上(安田)久美, シティマストゥラ, 都賀亮人, 伊藤-佐々木隆広, 珠玖 仁, 末永智一

    Chemical Sensors　34　67-69　2018

  5. バイポーラ電極を用いた新規測定システムの開発.

    池川未歩, 高野真一朗, 井上(安田)久美, 伊藤(佐々木, 隆広, 伊野浩介, 珠玖 仁, 末永智一

    Chemical Sensors　32　78-80　2016

  6. PREDICTION FOR DEVELOPMENTAL COMPETANCE OF HUMAN BLASTOCYST BASED ON ITS OXYGEN CONSUMPTION

    M. Yamanaka, S. Hashimoto, A. Amo, T. Ito-Sasaki, H. Abe, Y. Morimoto

    FERTILITY AND STERILITY　96　(3)　S73-S73　2011/09

    ISSN： 0015-0282

  7. Can we predict the developmental competence of frozen-thawed blastocyst by measuring respiratory activity?

    YAMANAKA Masaya, HASHIMOTO Shu, AMOU Ami, ITO Takahiro, ABE Hiroyuki, MORIMOTO Yoshiharu

    28　(2)　S87　2011/04/01

    ISSN： 1341-7738

  8. 有用な膵島評価法である呼吸活性計測法の実際

    後藤 昌史, 阿部 宏之, 伊藤 隆広, 佐々木, 後藤 めぐみ, 稲垣 明子, 小川 則彦, 山谷 英之, 高橋 英幸, 齋藤 之彦, 戸子台 和哲, Feng Qiang, 関口 悟, 藤盛 啓成, 黒川 良望, 里見 進

    移植　43　(総会臨時)　317-317　2008/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

  9. 非侵襲性呼吸計測技術の発展的活用による東北大式膵島評価法の確立

    後藤 昌史, 阿部 宏之, 伊藤 隆広, 佐々木, 後藤 めぐみ, 稲垣 明子, 猪村 武弘, 小川 則彦, 山谷 英之, 高橋 英幸, 齋藤 之彦, 戸子台 和哲, 関口 悟, 藤盛 啓成, 黒川 良望, 玉井 信, 里見 進

    移植　43　(3)　240-240　2008/06

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

  10. Development of a microwell culture device for individual embryo

    DATE Yasumoto, YOKOO Masaki, ITOH SASAKI Takahiro, SHIKU Hitoshi, ABE Hiroyuki, MATSUE Tomokazu

    24　(2)　S65　2007/04/01

    ISSN： 1341-7738

  11. Assessment of embryo culture medium for ART using by electrochemical respiration measuring system

    YOKOO Masaki, ITO SASAKI Takahiro, DATE Yasumoto, SHIKU Hitoshi, MATSUE Tomokazu, ABE Hiroyuki

    24　(2)　S49　2007/04/01

    ISSN： 1341-7738

  12. Analysis of mitochondrial function in mouse embryos during early embryonic development

    OKAZAKI Naoto, YOKOO Masaki, ITO SASAKI Takahiro, YASUKAWA Tomoyuki, SHIKU Hitoshi, ABE Hiroyuki, MATSUE Tomokazu

    24　(2)　S47　2007/04/01

    ISSN： 1341-7738

  13. The efficient maintenance of oxygen consumption by porcine oocytes relates to the high competence of oocyte maturation

    M. Yokoo, T. Ito-Sasaki, H. Shiku, T. Matsue, S. Aoyagi, H. Hoshi, H. Abe

    REPRODUCTION FERTILITY AND DEVELOPMENT　19　(1)　295-295　2007

    ISSN： 1031-3613

  14. Estimation of oxygen consumption of mouse oocytes during maturation by scanning electrochemical microscopy

    Takahiro Sasaki, Masaki Yokoo, Hiroyuki Abe

    ZOOLOGICAL SCIENCE　22　(12)　1453-1453　2005/12

    ISSN： 0289-0003

  15. Autonomous differentiation of primary spermatocytes into fertilizable sperm in the teleost, Oryzias Latipes

    A Watanabe, T Sasaki, E Takayama-Watanabe, K Onitake

    PROCEEDING OF 9TH INTERNATIONAL SYMPOSIUM ON SPERMATOLOGY　45-49　2002

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Presentations 3

  1. 世界初のＬＡＬ試薬を使用しないエンドトキシン検出装置

    伊藤 隆広

    新技術説明会　2021/07/27

  2. アプタマーを用いたアレルゲン検出デバイスの開発

    伊藤 隆広

    COI学会　2018/10/25

  3. マウス胚発生過程におけるCytochrome c oxidase遺伝子の発現変化

    伊藤-佐々木

    日本動物学会東北支部大会　2008/07/26

Industrial Property Rights 7

  1. race material measuremen;method using hydrolsse

    TOTSUKA Naoya, MASAKARI Yosue, MATSUE Tomokazu, INOUE Kumi, ITO Takahiro

    特許WO2021210669A1

    Property Type: Patent

  2. 穿刺システム、穿刺補助具、体表面照射レーザ機構及び穿刺ナビゲーションシステム

    宮口裕, 針谷綾花, 小黒草太, 伊藤隆広

    Property Type: Patent

  3. 電極チップ

    末永智一, 井上久美, 伊藤隆広, 珠玖仁, 池川未歩

    Property Type: Patent

  4. 核酸検出法

    川瀬三雄, 伊藤 隆広

    Property Type: Patent

  5. 哺乳動物受精卵の呼吸活性測定方法及びその電気化学デバイス

    珠玖 仁, 末永 智一, 高野 真一朗, 伊達 安基, 斉藤 剛史, 横尾 正樹, 伊藤 隆広, 阿部 宏之

    Property Type: Patent

  6. エンドトキシン検出装置、及びエンドトキシンの検出方法

    伊藤隆広、井上久美、伊藤健太郎、珠玖仁、末永智一

    Property Type: Patent

  7. 携帯型検査システム、アプタマーシート、および携帯型検査デバイス

    金子 直人, 堀井 克紀, 和賀 巌, 田中 秀治, 塚本 貴城, 井上 久美, 伊藤 隆広, アブデリ ハムザ

    Property Type: Patent

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Research Projects 3

  1. 感染拡大抑制に寄与する迅速細菌遺伝子検査キットの開発

    Offer Organization: 国立研究開発法人 科学技術振興機構

    System: COI若手連携研究ファンド

    2020/04 - 2021/03

  2. 感染拡大抑制に寄与する迅速細菌遺伝子検査キットの開発

    Offer Organization: 国立研究開発法人 科学技術振興機構

    System: COI若手連携研究ファンド

    2019/04 - 2020/03

  3. 次世代オンサイト簡易遺伝子検査法「RCA-PAS法」の開発 Competitive

    伊藤 隆広

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業若手研究（B)

    2015/04 - 2017/03