Details of the Researcher

PHOTO

Hideaki Yamamoto
Section
Research Institute of Electrical Communication
Job title
Associate Professor
Degree

  • 博士（工学）（早稲田大学）

  • 修士（工学）（早稲田大学）

Profile

JSPS Grant-in-Aid for Transformative Research Areas (A) "Multicellular Neurobiocomputing" https://www.mnbc.riec.tohoku.ac.jp/en/ (2024.4 - 2029.3)

JSPS Grant-in-Aid for Transformative Research Areas (B) "Multicellular Neurobiocomputing" https://www.mnbc.riec.tohoku.ac.jp/Areas_B/en/ (2021.4 - 2024.3)

Research History 11

  • 2020/01 - Present
    Tohoku University　Research Institute of Electrical Communication　Associate Professor

  • 2020/01 - Present
    Tohoku University　Advanced Institute for Materials Research　Associate Professor

  • 2020/01 - Present
    Tohoku University　Faculty of Engineering Department of Electrical,Information and Physics Engineering　Associate Professor

  • 2020/01 - Present
    Tohoku University　Graduate School of Engineering Department of Electronic Engineering　Associate Professor

  • 2018/10 - 2022/03
    JST　PRESTO Researcher

  • 2018/05 - 2019/12
    Tohoku University　Advanced Institute for Materials Research　Assistant Professor

  • 2018/05 - 2019/03
    Technical University of Munich　Institute for Advanced Study　Visiting Fellow

  • 2014/04 - 2018/04
    Tohoku University　Frontier Research Institute for Interdisciplinary Sciences　Assistant Professor

  • 2013/04 - 2014/03
    Waseda University　Waseda Institute for Advanced Study　Assistant Professor

  • 2010/04 - 2013/03
    Tokyo University of Agriculture and Technology　Faculty of Engineering Department of Biotechnology and Life Science　JSPS Research Fellow (SPD)

  • 2007/04 - 2010/03
    Waseda University　Graduate School of Advanced Science and Engineering　JSPS Research Fellow (DC1/PD)

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Education 3

  • Waseda University　Graduate School of Advanced Science and Engineering

    2007/04 - 2009/03

  • Waseda University　Graduate School of Science and Engineering

    2005/04 - 2007/03

  • Waseda University　School of Science and Engineering

    2001/04 - 2005/03

Committee Memberships 16

  • Japanese Neural Network Society　Representative

    2025/04 - Present

  • 応用物理学会　機関誌企画・編集委員会 委員

    2025/04 - Present

  • 文部科学省教科用図書検定調査審議会　臨時委員

    2022/04 - Present

  • Japan Nanomedicine Society　Board Member

    2017/04 - Present

  • Japanese Neural Network Society　Board Member

    2021/04 - 2025/03

  • 応用物理学会　プログラム編集委員

    2020/04 - 2024/09

  • IEEE World Congress on Computational Intelligence (WCCI) 2024　Exhibition Chair

    2022/07 - 2024/07

  • 2024 International Conference on Molecular Electronics & Bioelectronics (M&BE11)　Executive member

    2023/05 - 2024/06

  • 応用物理学会 有機分子・バイオエレクトロニクス分科会　幹事

    2020/04 - 2024/03

  • 31st International Conference on Artificial Neural Networks (ICANN2022)　Programme Committee

    2022/03 - 2022/09

  • 文部科学省教科用図書検定調査審議会　専門委員

    2020/10 - 2022/03

  • Nonlinear Theory and Its Applications (NOLTA), IEICE (Special Section on Nonlinear Dynamical Aspects of Edge Computing and Neuromorphic Hardware)　Guest Associate Editor

    2021/03 - 2021/10

  • 日本表面科学会　会誌電子化委員会 委員

    2008/07 - 2017/12

  • The 30th Annual Conference of Japanese Neural Network Society　Executive Committee Vice-Chair

    2020/12 -

  • 国際会議 11th International Symposium on Nanomedicine (11th ISNM)　運営委員

    2017/12 -

  • 国際会議 2017 International Conference on Solid State Devices and Materials (SSDM2017)　実行委員

    2017/12 -

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Professional Memberships 4

  • Japanese Neural Network Society

  • The Institute of Electronics, Information and Communication Engineers

  • The Japan Society of Vacuum and Surface Science

  • Japan Society of Applied Physics

Research Interests 8

  • bioelectronics

  • neuronal network

  • cultured neurons

  • self-assembled monolayer

  • biointerface

  • surface engineering

  • nanofabrication

  • Semiconductor process

Research Areas 2

  • Informatics / Biological, health, and medical informatics /

  • Nanotechnology/Materials / Nanobioscience /

Awards 9

  1. Research Encouragement Award

    2024/05　Miyagi Foundation for the Promotion of Industrial Science　Cell manipulation based on semiconductor technology and its neuroscience applications

  2. RIEC Award

    2024/02

  3. Best Paper Award

    2022/09　Molecular Electronics and Bioelectronics Division, Japan Society of Applied Physics　Impact of modular organization on dynamical richness in cortical networks

  4. Best Presentation Award

    2020/02　The Japan Society for Vacuum and Surface Science　In vitro engineering of neuronal network dynamics using microcontact-printed protein scaffolds

  5. 第23回青葉工学研究奨励賞

    2017/12　一般財団法人青葉工学振興会　バイオ界面設計に基づく実細胞操作による機能的神経回路の人工再構成

  6. トーキン財団奨励賞

    2017/03　公益財団法人トーキン科学技術振興財団

  7. 第74回応用物理学会秋季学術講演会 Poster Award（共同受賞）

    2013/09　応用物理学会　マイクロ加工基板上での単一神経細胞の長期培養

  8. 5th International Symposium on Surface Science and Nanotechnology (ISSS-5) Best Poster Prize

    2008/11　5th International Symposium on Surface Science and Nanotechnology (ISSS-5)　Enhancement of hydrofluoric acid resistivity in octadecylsilane self-assembled monolayers induced by post-deposition annealing

  9. 日本表面科学会 第27回表面科学講演大会 講演奨励賞（スチューデント部門）

    2008/02　日本表面科学会 第27回表面科学講演大会　大規模分子シミュレーションによる有機シランSAM/SiO2界面構造に関する研究

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Papers 102

  1. Precision Microfluidic Control of Neuronal Ensembles in Cultured Cortical Networks Peer-reviewed

    Hakuba Murota, Hideaki Yamamoto, Nobuaki Monma, Shigeo Sato, Ayumi Hirano-Iwata

    Advanced Materials Technologies　10　(4)　2400894　2025/02/19

    DOI： 10.1002/admt.202400894 　

    ISSN： 2365-709X

  2. Directional intermodular coupling enriches functional complexity in biological neuronal networks Peer-reviewed

    Nobuaki Monma, Hideaki Yamamoto, Naoya Fujiwara, Hakuba Murota, Satoshi Moriya, Ayumi Hirano-Iwata, Shigeo Sato

    Neural Networks　184　106967-106967　2024/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.neunet.2024.106967 　

    ISSN： 0893-6080

  3. In silico modeling of reservoir-based predictive coding in biological neuronal networks on microelectrode arrays Peer-reviewed

    Yuya Sato, Hideaki Yamamoto, Yoshitaka Ishikawa, Takuma Sumi, Yuki Sono, Shigeo Sato, Yuichi Katori, Ayumi Hirano-Iwata

    Japanese Journal of Applied Physics　63　(10)　108001　2024/10/07

    DOI： 10.35848/1347-4065/ad7ec1 　

    ISSN： 0021-4922

    eISSN： 1347-4065

  4. Enhanced responses to inflammatory cytokine interleukin-6 in micropatterned networks of cultured cortical neurons Peer-reviewed

    Mamoru Sakaibara, Hideaki Yamamoto, Hakuba Murota, Nobuyuki Monma, Shigeo Sato, Ayumi Hirano-Iwata

    Biochemical and Biophysical Research Communications　149379　2023/12/21

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbrc.2023.149379 　

    ISSN： 0006-291X

  5. Modular architecture facilitates noise-driven control of synchrony in neuronal networks Peer-reviewed

    Hideaki Yamamoto, F. Paul Spitzner, Taiki Takemuro, Victor Buendía, Hakuba Murota, Carla Morante, Tomohiro Konno, Shigeo Sato, Ayumi Hirano-Iwata, Anna Levina, Viola Priesemann, Miguel A. Muñoz, Johannes Zierenberg, Jordi Soriano

    Science Advances　9　(34)　eade1755　2023/08/25

    Publisher: American Association for the Advancement of Science (AAAS)

    DOI： 10.1126/sciadv.ade1755 　

    eISSN： 2375-2548

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    High-level information processing in the mammalian cortex requires both segregated processing in specialized circuits and integration across multiple circuits. One possible way to implement these seemingly opposing demands is by flexibly switching between states with different levels of synchrony. However, the mechanisms behind the control of complex synchronization patterns in neuronal networks remain elusive. Here, we use precision neuroengineering to manipulate and stimulate networks of cortical neurons in vitro, in combination with an in silico model of spiking neurons and a mesoscopic model of stochastically coupled modules to show that (i) a modular architecture enhances the sensitivity of the network to noise delivered as external asynchronous stimulation and that (ii) the persistent depletion of synaptic resources in stimulated neurons is the underlying mechanism for this effect. Together, our results demonstrate that the inherent dynamical state in structured networks of excitable units is determined by both its modular architecture and the properties of the external inputs.

  6. Biological neurons act as generalization filters in reservoir computing Peer-reviewed

    Takuma Sumi, Hideaki Yamamoto, Yuichi Katori, Koki Ito, Satoshi Moriya, Tomohiro Konno, Shigeo Sato, Ayumi Hirano-Iwata

    Proceedings of the National Academy of Sciences　120　(25)　2023/06/12

    Publisher: Proceedings of the National Academy of Sciences

    DOI： 10.1073/pnas.2217008120 　

    ISSN： 0027-8424

    eISSN： 1091-6490

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    Reservoir computing is a machine learning paradigm that transforms the transient dynamics of high-dimensional nonlinear systems for processing time-series data. Although the paradigm was initially proposed to model information processing in the mammalian cortex, it remains unclear how the nonrandom network architecture, such as the modular architecture, in the cortex integrates with the biophysics of living neurons to characterize the function of biological neuronal networks (BNNs). Here, we used optogenetics and calcium imaging to record the multicellular responses of cultured BNNs and employed the reservoir computing framework to decode their computational capabilities. Micropatterned substrates were used to embed the modular architecture in the BNNs. We first show that the dynamics of modular BNNs in response to static inputs can be classified with a linear decoder and that the modularity of the BNNs positively correlates with the classification accuracy. We then used a timer task to verify that BNNs possess a short-term memory of several 100 ms and finally show that this property can be exploited for spoken digit classification. Interestingly, BNN-based reservoirs allow categorical learning, wherein a network trained on one dataset can be used to classify separate datasets of the same category. Such classification was not possible when the inputs were directly decoded by a linear decoder, suggesting that BNNs act as a generalization filter to improve reservoir computing performance. Our findings pave the way toward a mechanistic understanding of information representation within BNNs and build future expectations toward the realization of physical reservoir computing systems based on BNNs.

  7. Impact of modular organization on dynamical richness in cortical networks. Peer-reviewed

    Yamamoto H, Moriya S, Ide K, Hayakawa T, Akima H, Sato S, Kubota S, Tanii T, Niwano M, Teller S, Soriano J, Hirano-Iwata A

    Science Advances　4　(11)　eaau4914　2018/11

    DOI： 10.1126/sciadv.aau4914 　

  8. Differential neurite outgrowth is required for axon specification by cultured hippocampal neurons Peer-reviewed

    Hideaki Yamamoto, Takanori Demura, Mayu Morita, Gary A. Banker, Takashi Tanii, Shun Nakamura

    JOURNAL OF NEUROCHEMISTRY　123　(6)　904-910　2012/12

    DOI： 10.1111/jnc.12001 　

    ISSN： 0022-3042

  9. Analog VLSI Implementation of Subthreshold Spiking Neural Networks and Its Application to Reservoir Computing Peer-reviewed

    Satoshi Moriya, Masaya Ishikawa, Satoshi Ono, Hideaki Yamamoto, Yasushi Yuminaka, Yoshihiko Horio, Jordi Madrenas, Shigeo Sato

    IEEE Transactions on Circuits and Systems I: Regular Papers　1-12　2025

    Publisher: Institute of Electrical and Electronics Engineers (IEEE)

    DOI： 10.1109/tcsi.2025.3550876 　

    ISSN： 1549-8328

    eISSN： 1558-0806

  10. Design of mixed-signal LSI with analog spiking neural network and digital inference circuits for reservoir computing, Peer-reviewed

    Satoshi Moriya, Hideaki Yamamoto, Masaya Ishikawa, Yasushi Yuminaka, Yoshihiko Horio, Jordi Madrenas, Shigeo Sato

    Proc. IEEE World Congress on Computational Intelligence　1-6　2024/06

    DOI： 10.1109/IJCNN60899.2024.10649999 　

  11. Numerical study on physical reservoir computing with Josephson junctions Peer-reviewed

    Kohki Watanabe, Yoshinao Mizugaki, Satoshi Moriya, Hideaki Yamamoto, Taro Yamashita, Shigeo Sato

    IEEE Transactions on Applied Superconductivity　34　(3)　1-4　2024/05

    Publisher: Institute of Electrical and Electronics Engineers (IEEE)

    DOI： 10.1109/tasc.2024.3350576 　

    ISSN： 1051-8223

    eISSN： 1558-2515 2378-7074

  12. Integrating predictive coding with reservoir computing in spiking neural network model of cultured neurons Peer-reviewed

    Yoshitaka Ishikawa, Takumi Shinkawa, Takuma Sumi, Hideyuki Kato, Hideaki Yamamoto, Yuichi Katori

    Nonlinear Theory and Its Applications, IEICE　2024

    DOI： 10.1587/nolta.15.432 　

  13. Two-dimensional water-molecule-cluster layers at nanobubble interfaces Peer-reviewed

    Michio Niwano, Teng Ma, Kazuki Iwata, Daisuke Tadaki, Hideaki Yamamoto, Yasuo Kimura, Ayumi Hirano-Iwata

    Journal of Colloid and Interface Science　652, Part B　1775-1783　2023/12/15

    Publisher: Elsevier BV

    DOI： 10.1016/j.jcis.2023.08.173 　

    ISSN： 0021-9797

  14. Analog hardware implementation of spiking neural networks for edge computing Peer-reviewed

    Satoshi Moriya, Hideaki Yamamoto, Shigeo Sato, Yasushi Yuminaka, Yoshihiko Horio, Jordi Madrenas

    Proc. International Symposium on Nonlinear Theory and Its Applications　317-318　2023/09

    DOI： 10.34385/proc.76.B3L-32 　

  15. Model-free idealization: Adaptive integrated approach for idealization of ion-channel currents Peer-reviewed

    Madoka Sato, Masanori Hariyama, Maki Komiya, Kae Suzuki, Yuzuru Tozawa, Hideaki Yamamoto, Ayumi Hirano-Iwata

    Biophysical Journal　122　(19)　3959-3975　2023/08/25

    Publisher: Elsevier BV

    DOI： 10.1016/j.bpj.2023.08.019 　

    ISSN： 0006-3495

  16. Microfluidic technologies for reconstituting neuronal network functions in vitro Invited

    Hideaki Yamamoto, Ayumi Hirano-Iwata, Shigeo Sato

    JSAP Review　2023　230420　2023/07/06

    DOI： 10.11470/jsaprev.230420 　

  17. マイクロ流体デバイスを用いた神経回路機能の実細胞再構成 Invited

    山本 英明, 平野 愛弓, 佐藤 茂雄

    応用物理　92　(5)　278-282　2023/05/01

    DOI： 10.11470/oubutsu.92.5_278 　

  18. Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks Peer-reviewed

    Yuya Sato, Hideaki Yamamoto, Hideyuki Kato, Takashi Tanii, Shigeo Sato, Ayumi Hirano-Iwata

    Frontiers in Neuroscience　16　943310　2023/01/09

    Publisher: Frontiers Media SA

    DOI： 10.3389/fnins.2022.943310 　

    eISSN： 1662-453X

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    Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined neuronal networks possessing a modular architecture on high-density microelectrode arrays (HD-MEAs), a state-of-the-art electrophysiological tool for recording neural activity with high spatial and temporal resolutions. We first established a surface coating protocol using a cell-permissive hydrogel to stably attach a polydimethylsiloxane microfluidic film on the HD-MEA. We then recorded the spontaneous neural activity of the engineered neuronal network, which revealed an important portrait of the engineered neuronal network–modular architecture enhances functional complexity by reducing the excessive neural correlation between spatially segregated modules. The results of this study highlight the impact of HD-MEA recordings combined with cell engineering technologies as a novel tool in neuroscience to constructively assess the structure-function relationships in neuronal networks.

  19. Time-Series Classification in Micropatterned Neuronal Network Reservoirs

    Takuma Sumi, Hideaki Yamamoto, Yuichi Katori, Koki Ito, Shigeo Sato, Ayumi Hirano-Iwata

    IEICE Proceeding Series　71　173-175　2022/12/12

    Publisher: The Institute of Electronics, Information and Communication Engineers

    DOI： 10.34385/proc.71.a5l-d-02 　

    eISSN： 2188-5079

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    Reservoir computing provides a novel framework to understand how the dynamics within biological neuronal networks (BNNs) is linked to information processing. Here, we used micropatterned substrates to fabricate BNNs with modular topology, one of the important structural features of brain networks, and realized a reservoir system with the modular BNN. Using image and time-series classification tasks, we evaluated the reservoir computing properties of the BNN reservoirs. The results show that modularity facilitates the separation between the trajectories of the neuronal responses to different spatial patterns, pointing to the functional advantage of the animals to modular topology within the nervous systems.

  20. Ultra-low power analog CMOS implementation of spiking neural networks for reservoir computing applications Peer-reviewed

    Satoshi Moriya, Hideaki Yamamoto, Shigeo Sato, Yasushi Yuminaka, Yoshihiko Horio, Jordi Madrenas

    Proceedings of International Symposium on Nonlinear Theory and Its Applications　171-172　2022/12

    DOI： 10.34385/proc.71.A5L-D-01 　

  21. マイクロパターン基板を用いた神経回路機能の実細胞再構成 Invited

    山本英明

    Molecular Electronics and Bioelectronics　33　(4)　185-189　2022/12

  22. A Fully Analog CMOS Implementation of a Two-variable Spiking Neuron in the Subthreshold Region and its Network Operation

    Satoshi Moriya, Hideaki Yamamoto, Shigeo Sato, Yasushi Yuminaka, Yoshihiko Horio, Jordi Madrenas

    2022 International Joint Conference on Neural Networks (IJCNN)　2022/07/18

    Publisher: IEEE

    DOI： 10.1109/ijcnn55064.2022.9891920 　

  23. An investigation of the relationship between numerical precision and performance of Q-learning for hardware implementation Peer-reviewed

    Daisuke Oguchi, Satoshi Moriya, Hideaki Yamamoto, Shigeo Sato

    Nonlinear Theory and Its Applications, IEICE　13　(2)　427-433　2022/04

    DOI： 10.1587/nolta.13.427 　

  24. Analog-circuit implementation of multiplicative spike-timing-dependent plasticity with linear decay Peer-reviewed

    Satoshi Moriya, Tatsuki Kato, Daisuke Oguchi, Hideaki Yamamoto, Shigeo Sato, Yasushi Yuminaka, Yoshihiko Horio, Jordi Madrenas

    Nonlinear Theory and Its Applications, IEICE　12　(4)　685-694　2021/10

    Publisher: Institute of Electronics, Information and Communications Engineers (IEICE)

    DOI： 10.1587/nolta.12.685 　

    eISSN： 2185-4106

  25. A Subthreshold Spiking Neuron Circuit Based on the Izhikevich Model Peer-reviewed

    Shigeo Sato, Satoshi Moriya, Yuka Kanke, Hideaki Yamamoto, Yoshihiko Horio, Yasushi Yuminaka, Jordi Madrenas

    Lecture Notes in Computer Science　12895　177-181　2021/09

    Publisher: Springer International Publishing

    DOI： 10.1007/978-3-030-86383-8_14 　

    ISSN： 0302-9743

    eISSN： 1611-3349

  26. Role of Noise in Spontaneous Activity of Networks of Neurons on Patterned Silicon Emulated by Noise--activated CMOS Neural Nanoelectronic Circuits Peer-reviewed

    Ramin Hasani, Giorgio Ferrari, Hideaki Yamamoto, Takashi Tanii, Enrico Prati

    Nano Express　2021/03/26

    Publisher: IOP Publishing

    DOI： 10.1088/2632-959x/abf2ae 　

    eISSN： 2632-959X

  27. Selective Stimulation of a Target Neuron in Micropatterned Neuronal Circuits Using a Pair of Needle Electrodes Peer-reviewed

    Kouhei Hattori, Hekiru Kurakake, Junko Imai, Takuya Hashimoto, Mihoko Ishida, Koki Sato, Honoka Takahashi, Soichiro Oguma, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii

    Electrochemistry　in press　2021/03

  28. Parallel recordings of hERG channel currents using a solvent-free lipid bilayer microarray

    Tadaki Daisuke, Miyata Ryusuke, Yamaura Daichi, Araki Shun, Sato Madoka, Komiya Maki, Ma Teng, Yamamoto Hideaki, Niwano Michio, Hirano-Iwata Ayumi

    JSAP Annual Meetings Extended Abstracts　2021.1　2147-2147　2021/02/26

    Publisher: The Japan Society of Applied Physics

    DOI： 10.11470/jsapmeeting.2021.1.0_2147 　

    eISSN： 2436-7613

  29. Reservoir computing properties of in-silico/in-vitro modular neuronal networks

    Sumi Takuma, Yamamoto Hideaki, Takemuro Taiki, Moriya Satoshi, Sato Shigeo, Hirano-Iwata Ayumi

    JSAP Annual Meetings Extended Abstracts　2021.1　2185-2185　2021/02/26

    Publisher: The Japan Society of Applied Physics

    DOI： 10.11470/jsapmeeting.2021.1.0_2185 　

    eISSN： 2436-7613

  30. Computational Efficiency of a Modular Reservoir Network for Image Recognition Peer-reviewed

    Yifan Dai, Hideaki Yamamoto, Masao Sakuraba, Shigeo Sato

    Frontiers in Computational Neuroscience　15　594337　2021/02/05

    Publisher: Frontiers Media SA

    DOI： 10.3389/fncom.2021.594337 　

    eISSN： 1662-5188

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    Liquid state machine (LSM) is a type of recurrent spiking network with a strong relationship to neurophysiology and has achieved great success in time series processing. However, the computational cost of simulations and complex dynamics with time dependency limit the size and functionality of LSMs. This paper presents a large-scale bioinspired LSM with modular topology. We integrate the findings on the visual cortex that specifically designed input synapses can fit the activation of the real cortex and perform the Hough transform, a feature extraction algorithm used in digital image processing, without additional cost. We experimentally verify that such a combination can significantly improve the network functionality. The network performance is evaluated using the MNIST dataset where the image data are encoded into spiking series by Poisson coding. We show that the proposed structure can not only significantly reduce the computational complexity but also achieve higher performance compared to the structure of previous reported networks of a similar size. We also show that the proposed structure has better robustness against system damage than the small-world and random structures. We believe that the proposed computationally efficient method can greatly contribute to future applications of reservoir computing.

  31. Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray Peer-reviewed

    Ryusuke Miyata, Daisuke Tadaki, Daichi Yamaura, Shun Araki, Madoka Sato, Maki Komiya, Teng Ma, Hideaki Yamamoto, Michio Niwano, Ayumi Hirano-Iwata

    Micromachines　12　(1)　98-98　2021/01/19

    Publisher: MDPI AG

    DOI： 10.3390/mi12010098 　

    eISSN： 2072-666X

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    The reconstitution of ion-channel proteins in artificially formed bilayer lipid membranes (BLMs) forms a well-defined system for the functional analysis of ion channels and screening of the effects of drugs that act on these proteins. To improve the efficiency of the BLM reconstitution system, we report on a microarray of stable solvent-free BLMs formed in microfabricated silicon (Si) chips, where micro-apertures with well-defined nano- and micro-tapered edges were fabricated. Sixteen micro-wells were manufactured in a chamber made of Teflon®, and the Si chips were individually embedded in the respective wells as a recording site. Typically, 11 to 16 BLMs were simultaneously formed with an average BLM number of 13.1, which corresponded to a formation probability of 82%. Parallel recordings of ion-channel activities from multiple BLMs were successfully demonstrated using the human ether-a-go-go-related gene (hERG) potassium channel, of which the relation to arrhythmic side effects following drug treatment is well recognized.

  32. Contribution of AMPA and NMDA receptors in the spontaneous firing patterns of single neurons in autaptic culture Peer-reviewed

    Kouhei Hattori, Takeshi Hayakawa, Akira Nakanishi, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii

    Biosystems　198　104278-104278　2020/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.biosystems.2020.104278 　

    ISSN： 0303-2647

  33. Modular networks of spiking neurons for applications in time-series information processing Peer-reviewed

    Satoshi Moriya, Hideaki Yamamoto, Ayumi Hirano-Iwata, Shigeru Kubota, Shigeo Sato

    Nonlinear Theory and Its Applications, IEICE　11　(4)　590-600　2020/10

    Publisher: Institute of Electronics, Information and Communications Engineers (IEICE)

    DOI： 10.1587/nolta.11.590 　

    eISSN： 2185-4106

  34. Polydimethylsiloxane microfluidic films for in vitro engineering of small-scale neuronal networks Peer-reviewed

    Taiki Takemuro, Hideaki Yamamoto, Shigeo Sato, Ayumi Hirano-Iwata

    Japanese Journal of Applied Physics　59　(11)　117001-117001　2020/10

    Publisher: IOP Publishing

    DOI： 10.35848/1347-4065/abc1ac 　

    ISSN： 0021-4922

    eISSN： 1347-4065

  35. Characterization of Bulk Nanobubbles Formed by Using a Porous Alumina Film with Ordered Nanopores Peer-reviewed

    Teng Ma, Yasuo Kimura, Hideaki Yamamoto, Xingyao Feng, Ayumi Hirano-Iwata, Michio Niwano

    The Journal of Physical Chemistry B　2020/06/04

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jpcb.0c02279 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  36. Impact of electrical field noise on micropatterned neuronal networks

    Sumi Takuma, Yamamoto Hideaki, Wakimura Kei, Sato Shigeo, Hirano-Iwata Ayumi

    JSAP Annual Meetings Extended Abstracts　2020.1　3653-3653　2020/02/28

    Publisher: The Japan Society of Applied Physics

    DOI： 10.11470/jsapmeeting.2020.1.0_3653 　

    eISSN： 2436-7613

  37. Suppression of hypersynchronous network activity in cultured cortical neurons using an ultrasoft silicone scaffold Peer-reviewed

    Takuma Sumi, Hideaki Yamamoto, Ayumi Hirano-Iwata

    Soft Matter　16　(13)　3195-3202　2020/02

    Publisher: Royal Society of Chemistry (RSC)

    DOI： 10.1039/c9sm02432h 　

    ISSN： 1744-683X

    eISSN： 1744-6848

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    <p>An ultrasoft silicone elastomer as an alternative substrate for neuronal culture is investigated.</p>

  38. Advances in Artificial Cell Membrane Systems as a Platform for Reconstituting Ion Channels Peer-reviewed

    Maki Komiya, Miki Kato, Daisuke Tadaki, Teng Ma, Hideaki Yamamoto, Ryugo Tero, Yuzuru Tozawa, Michio Niwano, Ayumi Hirano‐Iwata

    The Chemical Record　20　(7)　730-742　2020/01/16

    Publisher: Wiley

    DOI： 10.1002/tcr.201900094 　

    ISSN： 1527-8999

    eISSN： 1528-0691

  39. Culturing primary neurons on an ultrasoft silicone gel with biomimetic stiffness

    Sumi Takuma, Yamamoto Hideaki, Hirano-Iwata Ayumi

    JSAP Annual Meetings Extended Abstracts　2019.2　2852-2852　2019/09/04

    Publisher: The Japan Society of Applied Physics

    DOI： 10.11470/jsapmeeting.2019.2.0_2852 　

    eISSN： 2436-7613

  40. Ultrasoft silicone gel as a biomimetic passivation layer in inkjet‐printed 3D MEA devices International-journal Peer-reviewed

    Yamamoto H, Grob L, Sumi T, Oiwa K, Hirano-Iwata A, Wolfrum B

    Advanced Biosystems　3　(9)　1900130　2019/09

    DOI： 10.1002/adbi.201900130 　

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    Multielectrode arrays (MEAs) are versatile tools that are used for chronic recording and stimulation of neural cells and tissues. Driven by the recent progress in understanding of how neuronal growth and function respond to scaffold stiffness, development of MEAs with a soft cell-to-device interface has gained importance not only for in vivo but also for in vitro applications. However, the passivation layer, which constitutes the majority of the cell-device interface, is typically prepared with stiff materials. Herein, a fabrication of an MEA device with an ultrasoft passivation layer is described, which takes advantage of inkjet printing and a polydimethylsiloxane (PDMS) gel with a stiffness comparable to that of the brain. The major challenge in using the PDMS gel is that it cannot be patterned to expose the sensing area of the electrode. This issue is resolved by printing 3D micropillars at the electrode tip. Primary cortical neurons are grown on the fabricated device, and effective stimulation of the culture confirms functional cell-device coupling. The 3D MEA device with an ultrasoft interface provides a novel platform for investigating evoked activity and drug responses of living neuronal networks cultured in a biomimetic environment for both fundamental research and pharmaceutical applications.

  41. A Teflon-based system for applying multidirectional voltages to lipid bilayers as a novel platform for membrane proteins

    Maki Komiya, Kensaku Kanomata, Ryo Yokota, Yusuke Tsuneta, Madoka Sato, Daichi Yamaura, Daisuke Tadaki, Teng Ma, Hideaki Yamamoto, Yuzuru Tozawa, Albert Marti, Jordi Madrenas, Shigeru Kubota, Fumihiko Hirose, Michio Niwano, Ayumi Hirano-Iwata

    2019/07/12

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    Artificial bilayer lipid membranes (BLMs), along with patch-clamped<br /> membranes, are frequently used for functional analyses of membrane proteins. In<br /> both methods, the electric properties of membranes are characterized by only<br /> one parameter, namely, transmembrane potential. Here the construction of a<br /> novel BLM system was reported, in which membrane voltages can be controlled in<br /> a lateral direction in addition to conventional transmembrane direction. A<br /> microaperture was fabricated in a Teflon film and Ti electrodes were evaporated<br /> around the aperture. BLMs were reproducibly formed in the aperture without<br /> being affected by the presence of the electrodes. The application of a lateral<br /> voltage induced no significant changes in the electric properties of the BLMs,<br /> such as baseline current, transmembrane resistance, and transmembrane<br /> capacitance. In contrast, lateral voltages clearly affected the activities of<br /> biological ion channels, suggesting that the lateral voltage might be a useful<br /> parameter for analyzing channel activities. The present Teflon-based system in<br /> which multidirectional voltages can be applied to BLMs represent a promising<br /> platform for the analysis of underlying functional properties of membrane<br /> proteins.

  42. Analysis of ion channel openings using bilayer lipid membranes

    Yokota Ryo, Hirano-Iwata Ayumi, Tsuneta Yusuke, Sato Madoka, Kato Miki, Yamaura Daichi, Tadaki Daisuke, Komiya Maki, Yamamoto Hideaki, Niwano Michio

    Abstract book of Annual Meeting of the Japan Society of Vacuum and Surface Science　2019　(0)　2P56　2019

    Publisher: The Japan Society of Vacuum and Surface Science

    DOI： 10.14886/jvss.2019.0_2P56 　

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  43. Printed 3D electrode arrays with micrometer-scale lateral resolution for extracellular recording of action potentials Peer-reviewed

    Grob L, Yamamoto H, Zips S, Rinklin P, Hirano-Iwata A, Wolfrum B

    Advanced Materials Technologies　4　1900517　2019

    DOI： 10.1002/admt.201900517 　

  44. Mean-field analysis of directed modular networks. Peer-reviewed

    Moriya S, Yamamoto H, Akima H, Hirano-Iwata A, Kubota S, Sato S

    Chaos: An Interdisciplinary Journal of Nonlinear Science　29　013142　2019/01

    DOI： 10.1063/1.5044689 　

  45. A single-cell based hybrid neuronal network configured by integration of cell micropatterning and dynamic patch-clamp Peer-reviewed

    Yamamoto H, Hayakawa T, Netoff TI, Hirano-Iwata A

    Applied Physics Letters　113　(13)　133703　2018/09

    DOI： 10.1063/1.5049487 　

  46. Dependence and Homeostasis of Membrane Impedance on Cell Morphology in Cultured Hippocampal Neurons. Peer-reviewed

    Matsumura R, Yamamoto H, Hayakawa T, Katsurabayashi S, Niwano M, Hirano-Iwata A

    Scientific Reports　8　9905　2018/07

    DOI： 10.1038/s41598-018-28232-0 　

  47. Amphiphobic Septa Enhance the Mechanical Stability of Free-Standing Bilayer Lipid Membranes Peer-reviewed

    Daichi Yamaura, Daisuke Tadaki, Shun Araki, Miyu Yoshida, Kohei Arata, Takeshi Ohori, Ken-ichi Ishibashi, Miki Kato, Teng Ma, Ryusuke Miyata, Hideaki Yamamoto, Ryugo Tero, Masao Sakuraba, Toshio Ogino, Michio Niwano, Ayumi Hirano-Iwata

    LANGMUIR　34　(19)　5615-5622　2018/05

    DOI： 10.1021/acs.langmuir.8b00747 　

    ISSN： 0743-7463

  48. Effective subnetwork topology for synchronizing interconnected networks of coupled phase oscillators Peer-reviewed

    Yamamoto H, Kubota S, Shimizu FA, Hirano-Iwata A, Niwano M

    Front Comput Neurosci　12　17　2018/03

    DOI： 10.3389/fncom.2018.00017 　

  49. Micro- and nanofabrication methods for ion channel reconstitution in bilayer lipid membranes Invited Peer-reviewed

    Daisuke Tadaki, Daichi Yamaura, Kohei Arata, Takeshi Ohori, Teng Ma, Hideaki Yamamoto, Michio Niwano, Ayumi Hirano-Iwata

    Japanese Journal of Applied Physics　57　(3)　03EA01　2018/03/01

    Publisher: Japan Society of Applied Physics

    DOI： 10.7567/JJAP.57.03EA01 　

    ISSN： 1347-4065 0021-4922

    eISSN： 1347-4065

  50. In situ modification of cell-culture scaffolds by photocatalysis of visible-light-responsive TiO2 film Peer-reviewed

    Sho Kono, Kohei Furusawa, Atsushi Kurotobi, Kohei Hattori, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii

    Japanese Journal of Applied Physics　57　(2)　027001　2018/02/01

    Publisher: Japan Society of Applied Physics

    DOI： 10.7567/JJAP.57.027001 　

    ISSN： 1347-4065 0021-4922

  51. Biomimetic culture of primary neurons using ultrasoft gels

    Sumi Takuma, Yamamoto Hideaki, Hayakawa Takeshi, Ide Katsuya, Kino Hisashi, Tanaka Tetsu, Hirano Ayumi

    Abstract of annual meeting of the Surface Science of Japan　2018　180　2018

    Publisher: The Japan Society of Vacuum and Surface Science

    DOI： 10.14886/sssj2008.2018.0_180 　

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    Mechanical properties of scaffolds have recently been found to impose strong impact on the behavior of cultured cells. Here, we focused on the effect of scaffold’s elastic modulus on the development of synaptic connections in cultured neurons, and established a novel system for culturing rat cortical neurons on an ultrasolft gel surface with an elastic modulus resembling the brain. Impact of the soft scaffold on the amplitude of synaptic currents and spontaneous neuronal activity, analyzed via patch -clamp recording and fluorescence calcium imaging, will also be presented.

  52. Quantification of Drug Side Effects on Cell-Free Synthesized Ion-Channels Reconstituted in Bilayer Lipid Membranes

    Kato Miki, Hirano Ayumi, Inoue Haruka, Yamaura Daichi, Yokota Reo, Komiya Maki, Tadaki Daisuke, Yamamoto Hidaka, Tozawa Yuzuru, Niwano Michio

    Abstract of annual meeting of the Surface Science of Japan　2018　(0)　181-181　2018

    Publisher: The Japan Society of Vacuum and Surface Science

    DOI： 10.14886/sssj2008.2018.0_181 　

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    We aim to develop a drug screening system based on a reconstitution system in which ion channels are embedded in an artificial lipid bilayer membrane (BLM). We have been developing stable BLMs based on silicon (Si) microfabrication. We previously reported on efficient incorporation of ion channels in BLMs. Here we applied this system to a cell-free synthesized hERG channel and tried to quantify drug side effects on this channel.

  53. Astrocytes with previous chronic exposure to amyloid β-peptide fragment 1-40 suppress excitatory synaptic transmission. International-journal Peer-reviewed

    Hiroyuki Kawano, Kohei Oyabu, Hideaki Yamamoto, Kei Eto, Yuna Adaniya, Kaori Kubota, Takuya Watanabe, Ayumi Hirano-Iwata, Junichi Nabekura, Shutaro Katsurabayashi, Katsunori Iwasaki

    Journal of neurochemistry　143　(6)　624-634　2017/12

    DOI： 10.1111/jnc.14247 　

    More details Close

    Synaptic dysfunction and neuronal death are responsible for cognitive and behavioral deficits in Alzheimer's disease (AD). It is well known that such neurological abnormalities are preceded by long-term exposure of amyloid β-peptide (Aβ) and/or hyperphosphorylated tau prior. In addition to the neurological deficit, astrocytes as a major glial cell type in the brain, significantly participate in the neuropathogenic mechanisms underlying synaptic modulation. Although astrocytes play a significant key role in modulating synaptic transmission, little is known on whether astrocyte dysfunction caused by such long-term Aβ exposure affects synapse formation and function. Here, we show that synapse formation and synaptic transmission are attenuated in hippocampal-naïve neurons co-cultured with astrocytes that have previously experienced chronic Aβ1-40 exposure. In this abnormal astrocytic condition, hippocampal neurons exhibit decrements of evoked excitatory post-synaptic currents (EPSCs) and miniature EPSC frequency. Furthermore, size of readily releasable synaptic pools and number of excitatory synapses were also significantly decreased. Contrary to these negative effects, release probability at individual synapses was significantly increased in the same astrocytic condition. Taken together, our data indicate that lower synaptic transmission caused by astrocytes previously, and chronically, exposed to Aβ1-40 is attributable to a small number of synapses with higher release probability.

  54. Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels Peer-reviewed

    Daisuke Tadaki, Daichi Yamaura, Shun Araki, Miyu Yoshida, Kohei Arata, Takeshi Ohori, Ken-ichi Ishibashi, Miki Kato, Teng Ma, Ryusuke Miyata, Yuzuru Tozawa, Hideaki Yamamoto, Michio Niwano, Ayumi Hirano-Iwata

    SCIENTIFIC REPORTS　7　17736　2017/12

    DOI： 10.1038/s41598-017-17905-x 　

    ISSN： 2045-2322

  55. モジュール構造型神経回路モデルにおける同期活動のメカニズム

    守谷 哲, 山本英明, 井手克哉, 秋間学尚, 平野愛弓, 庭野道夫, 久保田 繁, 佐藤茂雄

    信学技報　117　(325)　19-23　2017/11

    Publisher:

    ISSN： 0913-5685

  56. Modularity-dependent modulation of synchronized bursting activity in cultured neuronal network models Peer-reviewed

    Satoshi Moriya, Hideaki Yamamoto, Hisanao Akima, Ayumi Hirano-Iwata, Michio Niwano, Shigeru Kubota, Shigeo Sato

    Proceedings of the International Joint Conference on Neural Networks　2017-　1163-1168　2017/06/30

    Publisher: Institute of Electrical and Electronics Engineers Inc.

    DOI： 10.1109/IJCNN.2017.7965983 　

  57. 半導体微細加工に基づく薬物副作用センサの開発

    平野愛弓, 但木大介, 山浦大地, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫

    信学技報　117　(6)　33-35　2017/04

    Publisher:

    ISSN： 0913-5685

  58. 自己組織化単分子膜による自立型脂質二分子膜の膜特性制御

    山浦大地, 但木大介, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫, 平野愛弓

    信学技報　117　(6)　41-42　2017/04

    ISSN： 0913-5685

  59. 安定な人工脂質二分子膜形成のためのテーパー構造を有した微細孔をもつ半導体シリコンチップの作製

    但木大介, 山浦大地, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫, 平野愛弓

    信学技報　117　(6)　43-45　2017/04

    Publisher:

    ISSN： 0913-5685

  60. Functional integration of human ion channels in artificially formed bilayer lipid membranes

    Hirano-Iwata Ayumi, Tadaki Daisuke, Yamaura Daichi, Araki Shun, Yoshida Miyu, Arata Kohei, Ohori Takeshi, Yamamoto Hideaki, Niwano Michio

    JSAP Annual Meetings Extended Abstracts　2017.1　228-228　2017/03/01

    Publisher: The Japan Society of Applied Physics

    DOI： 10.11470/jsapmeeting.2017.1.0_228 　

    eISSN： 2436-7613

  61. Control of the correlation of spontaneous neuron activity in biological and noise-activated CMOS artificial neural microcircuits.

    Hasani RM, Ferrari G, Yamamoto H, Kono S, Ishihara K, Fujimori S, Tanii T, Prati E

    arXiv　1702.07426　2017/02/24

  62. Stabilization of artificial cell membranes based on nano- and micro-structures

    Hirano-Iwata Ayumi, Tadaki Daisuke, Ohori Takeshi, Arata Kohei, Ma Teng, Yamamoto Hideaki, Niwano Michio

    Abstract of annual meeting of the Surface Science of Japan　37　(0)　297-297　2017

    Publisher: The Surface Science Society of Japan

    DOI： 10.14886/sssj2008.37.0_297 　

  63. Reconstitution of human ion channels in lipid bilayers formed in microfabricated apertures

    Hirano-Iwata Ayumi, Yoshida Miyu, Araki Shun, Tadaki Daisuke, Ishibashi Kenichi, Arata Kohei, Takeshi Ohori, Yamamoto Hideaki, Michio Niwano

    JSAP Annual Meetings Extended Abstracts　2016.2　133-133　2016/09/01

    Publisher: The Japan Society of Applied Physics

    DOI： 10.11470/jsapmeeting.2016.2.0_133 　

    eISSN： 2436-7613

  64. Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: an experimental study on the anti-adhesion effect of green-tea catechins. International-journal Peer-reviewed

    Rumi Sakamoto, Eisuke Kakinuma, Kentaro Masuda, Yuko Takeuchi, Kosaku Ito, Kentaro Iketaki, Takahisa Matsuzaki, Seiichiro Nakabayashi, Hiroshi Y Yoshikawa, Hideaki Yamamoto, Yuko Sato, Takashi Tanii

    In vitro cellular & developmental biology. Animal　52　(8)　799-805　2016/09

    DOI： 10.1007/s11626-016-0049-6 　

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    The main constituent of green tea, (-)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (-)-Epicatechin-3-O-gallate (ECG) and (-)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion-suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells-and validates the use of OMT as a tool for screening cancer cell adhesion.

  65. Live-Cell, Label-Free Identification of GABAergic and Non-GABAergic Neurons in Primary Cortical Cultures Using Micropatterned Surface Peer-reviewed

    Sho Kono, Hideaki Yamamoto, Takatoshi Kushida, Ayumi Hirano-Iwata, Michio Niwano, Takashi Tanii

    PLOS ONE　11　(8)　e0160987　2016/08

    DOI： 10.1371/journal.pone.0160987 　

    ISSN： 1932-6203

  66. Size-dependent regulation of synchronized activity in living neuronal networks Peer-reviewed

    Hideaki Yamamoto, Shigeru Kubota, Yudai Chida, Mayu Morita, Satoshi Moriya, Hisanao Akima, Shigeo Sato, Ayumi Hirano-Iwata, Takashi Tanii, Michio Niwano

    PHYSICAL REVIEW E　94　(1)　012407　2016/07

    DOI： 10.1103/PhysRevE.94.012407 　

    ISSN： 2470-0045

    eISSN： 2470-0053

  67. Unidirectional signal propagation in primary neurons micropatterned at a single-cell resolution Peer-reviewed

    H. Yamamoto, R. Matsumura, H. Takaoki, S. Katsurabayashi, A. Hirano-Iwata, M. Niwano

    APPLIED PHYSICS LETTERS　109　(4)　043703　2016/07

    DOI： 10.1063/1.4959836 　

    ISSN： 0003-6951

    eISSN： 1077-3118

  68. 神経回路の同期的活動に対するモジュール構造の影響に関する計算論的研究

    守谷 哲, 山本英明, 秋間学尚, 平野愛弓, 庭野道夫, 久保田 繁, 佐藤茂雄

    信学技報　116　(120)　217-222　2016/06/28

  69. Reconstitution of Human Ion Channels into Solvent-free Lipid Bilayers Enhanced by Centrifugal Forces Peer-reviewed

    Ayumi Hirano-Iwata, Yutaka Ishinari, Miyu Yoshida, Shun Araki, Daisuke Tadaki, Ryusuke Miyata, Kenichi Ishibashi, Hideaki Yamamoto, Yasuo Kimura, Michio Niwano

    BIOPHYSICAL JOURNAL　110　(10)　2207-2215　2016/05

    DOI： 10.1016/j.bpj.2016.04.010 　

    ISSN： 0006-3495

    eISSN： 1542-0086

  70. 微細加工脂質二分子膜チップへのイオンチャネルの包埋

    平野愛弓, 吉田美優, 荒木 駿, 但木大介, 山本英明, 庭野道夫

    信学技報　116　(14)　1-3　2016/04/14

    Publisher:

    ISSN： 0913-5685

  71. 安定な人工脂質二分子膜形成のための微細孔を有するシリコンチップの作製

    但木大介, 平野愛弓, 石橋健一, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫

    信学技報　116　(14)　5-7　2016/04/14

    Publisher:

    ISSN： 0913-5685

  72. Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy Peer-reviewed

    Takahisa Matsuzaki, Kosaku Ito, Kentaro Masuda, Eisuke Kakinuma, Rumi Sakamoto, Kentaro Iketaki, Hideaki Yamamoto, Masami Suganuma, Naritaka Kobayashi, Seiichiro Nakabayashi, Takashi Tanii, Hiroshi Y. Yoshikawa

    JOURNAL OF PHYSICAL CHEMISTRY B　120　(7)　1221-1227　2016/02

    DOI： 10.1021/acs.jpcb.5b11870 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  73. Controlling stability of a free-standing lipid bilayer by surface modification with self-assembled monolayers.

    Yamaura Daichi, Tadaki Daisuke, Araki Syun, Yoshida Miu, Arata Kohei, Yamamoto Hideaki, Ogino Toshio, Niwano Michio, Hirano Ayumi

    Abstract of annual meeting of the Surface Science of Japan　36　41-41　2016

    Publisher: The Surface Science Society of Japan

    DOI： 10.14886/sssj2008.36.0_41 　

  74. Development of an artificial cell membrane array based on silicon microfabrication

    Araki Shun, Hirano-Iwata Ayumi, Miyata Ryusuke, Tadaki Daisuke, Ishibashi Kenichi, Yamamoto Hideaki, Niwano Michio

    Abstract of annual meeting of the Surface Science of Japan　36　146-146　2016

    Publisher: The Surface Science Society of Japan

    DOI： 10.14886/sssj2008.36.0_146 　

  75. Neuraminidase-Dependent Degradation of Polysialic Acid Is Required for the Lamination of Newly Generated Neurons Peer-reviewed

    Mari Sajo, Hiroki Sugiyama, Hideaki Yamamoto, Takashi Tanii, Norio Matsuki, Yuji Ikegaya, Ryuta Koyama

    PLOS ONE　11　(1)　e0146398　2016/01

    DOI： 10.1371/journal.pone.0146398 　

    ISSN： 1932-6203

  76. An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings Peer-reviewed

    R. Matsumura, H. Yamamoto, M. Niwano, A. Hirano-Iwata

    APPLIED PHYSICS LETTERS　108　(2)　023701　2016/01

    DOI： 10.1063/1.4939629 　

    ISSN： 0003-6951

    eISSN： 1077-3118

  77. 位相振動子がつくる複雑ネットワークにおける同期状態の解析

    清水 ファビオ アキラ, 山本英明, 千田雄大, 平野愛弓, 久保田 繁, 庭野道夫

    信学技報　115　(318)　1-5　2015/11/13

    Publisher:

    ISSN： 0913-5685

  78. 積分発火型ニューロン回路におけるスモールワールド構造と同期の関係

    千田雄大, 山本英明, 平野愛弓, 久保田 繁, 庭野道夫

    信学技報　115　(318)　7-12　2015/11/13

  79. Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis Invited Peer-reviewed

    Hideaki Yamamoto, Takanori Demura, Kohei Sekine, Sho Kono, Michio Niwano, Ayumi Hirano-Iwata, Takashi Tanii

    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS　104　(104)　e53045　2015/10

    DOI： 10.3791/53045 　

    ISSN： 1940-087X

  80. Micro- and nano-technologies for lipid bilayer-based ion-channel functional assays Invited Peer-reviewed

    Ayumi Hirano-Iwata, Yutaka Ishinari, Hideaki Yamamoto, Michio Niwano

    Chemistry - An Asian Journal　10　(6)　1266-1274　2015/06/01

    Publisher: John Wiley and Sons Ltd

    DOI： 10.1002/asia.201403391 　

    ISSN： 1861-471X 1861-4728

  81. 微細加工シリコン基板に基づくイオンチャネル機能の再構成

    平野愛弓, 山本英明, 木村康男, 庭野道夫

    信学技報　115　(5)　35-39　2015/04/09

    Publisher:

    ISSN： 0913-5685

  82. 赤外分光法によるナノバブルの表面洗浄効果のその場観察

    齋藤栄幸, 馬 騰, 山本英明, 平野愛弓, 庭野道夫

    信学技報　115　(5)　47-52　2015/04/09

  83. Formation of artificial cell membranes based on microfabricated silicon substrates

    Araki Shun, Hirano Ayumi, Yamamoto Hideaki, Niwano Michio

    Abstract of annual meeting of the Surface Science of Japan　35　172-172　2015

    Publisher: The Surface Science Society of Japan

    DOI： 10.14886/sssj2008.35.0_172 　

  84. In-situ observation of reactions of water cluster ions with polypeptides by infrared absorption spectroscopy

    Yoshida Mao, Ma Teng, Yamamoto Hideaki, Hirano Ayumi, Niwano Michio

    Abstract of annual meeting of the Surface Science of Japan　35　406-406　2015

    Publisher: The Surface Science Society of Japan

    DOI： 10.14886/sssj2008.35.0_406 　

  85. Generation of nanobubbles by porous alumina thin films

    Yamada Natsuki, Saito Hideyuki, Ma Teng, Hirano Ayumi, Yamamoto Hideaki, Ishibashi Ken-ichi, Miyazawa Makoto, Sakamoto Hitoshi, Niwano Michio

    Abstract of annual meeting of the Surface Science of Japan　35　281-281　2015

    Publisher: The Surface Science Society of Japan

    DOI： 10.14886/sssj2008.35.0_281 　

  86. Surface micromodification techniques for engineering cultured neurons and neuronal networks

    Yamamoto Hideaki, Hirano-Iwata Ayumi, Tanii Takashi, Niwano Michio

    Abstract of annual meeting of the Surface Science of Japan　35　21-21　2015

    Publisher: The Surface Science Society of Japan

    DOI： 10.14886/sssj2008.35.0_21 　

  87. Surface modification of cell scaffold in aqueous solution using TiO&lt;inf&gt;2&lt;/inf&gt; photocatalysis and linker protein L2 for patterning primary neurons Peer-reviewed

    Kohei Sekine, Hideaki Yamamoto, Sho Kono, Takeshi Ikeda, Akio Kuroda, Takashi Tanii

    e-Journal of Surface Science and Nanotechnology　13　213-218　2015

    Publisher: Surface Science Society of Japan

    DOI： 10.1380/ejssnt.2015.213 　

    ISSN： 1348-0391

  88. In situ modification of cell-culture scaffolds by photocatalytic decomposition of organosilane monolayers Peer-reviewed

    Hideaki Yamamoto, Takanori Demura, Mayu Morita, Sho Kono, Kohei Sekine, Takahiro Shinada, Shun Nakamura, Takashi Tanii

    BIOFABRICATION　6　(3)　035021　2014/09

    DOI： 10.1088/1758-5082/6/3/035021 　

    ISSN： 1758-5082

    eISSN： 1758-5090

  89. 微細加工シリコン基板とイオンチャネルの融合

    石成 裕, 平野 愛弓, 山本 英明, 木村 康男, 庭野 道夫

    表面科学学術講演会要旨集　34　60-60　2014

    Publisher: 公益社団法人 日本表面科学会

    DOI： 10.14886/sssj2008.34.0_60 　

    More details Close

    膜タンパク質の一つであるイオンチャネルは高感度な分子認識機能を有し，創薬における主ターゲットになっている。我々はイオンチャネルを人工脂質二分子膜に包埋した再構成系に基づく薬物スクリーニングチップの開発を目指しており，半導体微細加工との融合による安定な脂質二分子膜の構築について報告してきた。今回は，この安定化脂質二分子膜中への幾つかのイオンチャネル包埋について検討したので，ここに報告する。

  90. 1SDA-02 Manipulating neurons and neuronal networks with micropatterned surfaces(1SDA Measurement and control of neurodynamics,Symposium,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Yamamoto Hideaki, Tanii Takashi, Niwano Michio, Hirano-Iwata Ayumi

    Seibutsu Butsuri　54　(1)　S118　2014

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.54.S118_1 　

  91. Improving zero-mode waveguide structure for enhancing signal-to-noise ratio of real-time single-molecule fluorescence imaging: A computational study Peer-reviewed

    Takashi Tanii, Rena Akahori, Shun Higano, Kotaro Okubo, Hideaki Yamamoto, Taro Ueno, Takashi Funatsu

    Physical Review E - Statistical, Nonlinear, and Soft Matter Physics　88　(1)　012727　2013/07/30

    DOI： 10.1103/PhysRevE.88.012727 　

    ISSN： 1539-3755 1550-2376

  92. In situ laser micropatterning of proteins for dynamically arranging living cells Peer-reviewed

    Kazunori Okano, Ai Matsui, Yasuyo Maezawa, Ping-Yu Hee, Mie Matsubara, Hideaki Yamamoto, Yoichiroh Hosokawa, Hiroshi Tsubokawa, Yaw-Kuen Li, Fu-Jen Kao, Hiroshi Masuhara

    LAB ON A CHIP　13　(20)　4078-4086　2013

    DOI： 10.1039/c3lc50750e 　

    ISSN： 1473-0197

  93. In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers Peer-reviewed

    Hideaki Yamamoto, Kazunori Okano, Takanori Demura, Yoichiroh Hosokawa, Hiroshi Masuhara, Takashi Tanii, Shun Nakamura

    APPLIED PHYSICS LETTERS　99　(16)　163701　2011/10

    DOI： 10.1063/1.3651291 　

    ISSN： 0003-6951

  94. Application of Organosilane Monolayer Template to Quantitative Evaluation of Cancer Cell Adhesive Ability Peer-reviewed

    Takashi Tanii, Kosuke Sasaki, Kota Ichisawa, Takanori Demura, Yuichi Beppu, Hoan Anh Vu, Hoan Thanh Chi, Hideaki Yamamoto, Yuko Sato

    JAPANESE JOURNAL OF APPLIED PHYSICS　50　(6)　06GL01　2011/06

    DOI： 10.1143/JJAP.50.06GL01 　

    ISSN： 0021-4922

  95. Adsorption Mechanism of Ribosomal Protein L2 onto a Silica Surface: A Molecular Dynamics Simulation Study Peer-reviewed

    Ryo Tosaka, Hideaki Yamamoto, Iwao Ohdomari, Takanobu Watanabe

    LANGMUIR　26　(12)　9950-9955　2010/06

    DOI： 10.1021/la1004352 　

    ISSN： 0743-7463

  96. Green tea epigalocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via inhibition of both FAK and IGF-1R Peer-reviewed

    H. A. Vu, Y. Beppu, H. T. Chi, K. Sasaki, H. Yamamoto, P. T. Xinh, T. Tanii, Y. Hara, T. Watanabe, Y. Sato, I. Ohdomari

    IFMBE Proceedings　27　223-226　2010

    DOI： 10.1007/978-3-642-12020-6-56 　

    ISSN： 1680-0737

  97. Green Tea Epigallocatechin Gallate Exhibits Anticancer Effect in Human Pancreatic Carcinoma Cells via the Inhibition of Both Focal Adhesion Kinase and Insulin-Like Growth Factor-I Receptor Peer-reviewed

    Hoang Anh Vu, Yuuichi Beppu, Hoang Thanh Chi, Kousuke Sasaki, Hideaki Yamamoto, Phan Thi Xinh, Takashi Tanii, Yukihiko Hara, Toshiki Watanabe, Yuko Sato, Iwao Ohdomari

    JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY　2010　290516　2010

    DOI： 10.1155/2010/290516 　

    ISSN： 1110-7243

    eISSN： 1110-7251

  98. In-plane X-ray Diffraction Profiles from Organosilane Monolayer/SiO2 Models Peer-reviewed

    Hideaki Yamamoto, Takanobu Watanabe, Iwao Ohdomari

    APPLIED PHYSICS EXPRESS　1　(10)　105002　2008/10

    DOI： 10.1143/APEX.1.105002 　

    ISSN： 1882-0778

  99. A molecular simulation study of an organosilane self-assembled monolayer/SiO(2) substrate interface Peer-reviewed

    Hideaki Yamamoto, Takanobu Watanabe, Iwao Ohdomari

    JOURNAL OF CHEMICAL PHYSICS　128　(16)　164710　2008/04

    DOI： 10.1063/1.2895052 　

    ISSN： 0021-9606

  100. Structural investigation of organosilane self-assembled monolayers by atomic scale simulation Peer-reviewed

    H Yamamoto, T Watanabe, K Nishiyama, K Tatsumura, Ohdomari, I

    JOURNAL DE PHYSIQUE IV　132　189-193　2006/03

    DOI： 10.1051/jp4:2006132036 　

    ISSN： 1155-4339

  101. Reactions and diffusion of atomic and molecular oxygen in the SiO2 network Peer-reviewed

    K Tatsumura, T Shimura, E Mishima, K Kawamura, D Yamasaki, H Yamamoto, T Watanabe, M Umeno, Ohdomari, I

    PHYSICAL REVIEW B　72　(4)　045205　2005/07

    DOI： 10.1103/PhysRevB.72.045205 　

    ISSN： 1098-0121

  102. Quantitativecomparisonofcancerandnormalcelladhesionusingorganosilanemonolayertemplates:Anexperimentalstudyontheanti-adhesioneffectofgreen-teacatechins

    R.Sakamoto, E.Kakinuma, K.Masuda, Y.Takeuchi, K.Ito, K.Iketaki, T.Matsuzaki, S.Nakabayashi, H.Y.Yoshikawa, H.Yamamoto, Y.Sato, T.Tanii

    InVitroCell.Dev.Biol.-Animal52(2016)799-805.

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Misc. 37

  1. Network Structure and Reservoir Computing in Cultured Neuronal Networks Invited

    Takuma Sumi, Hideaki Yamamoto, Hayato Chiba, Yuichi Katori, Ayumi Hirano-Iwata

    日本神経回路学会誌　31　(3)　131-140　2024/09/05

    DOI： 10.3902/jnns.31.131 　

    ISSN： 1340-766X

  2. Biomimetic Culture of Primary Neurons on an Ultrasoft Gel Surface Peer-reviewed

    Takuma SUMI, Hideaki YAMAMOTO, Ayumi HIRANO-IWATA

    Vacuum and Surface Science　63　(6)　298-303　2020/06/10

    Publisher: Surface Science Society Japan

    DOI： 10.1380/vss.63.298 　

    ISSN： 2433-5835

    eISSN： 2433-5843

  3. 脳組織に近い弾性率を有するシリコーン樹脂の生体界面材料応用

    山本英明, 住 拓磨, 佐藤茂雄, 平野愛弓

    Molecular Electronics and Bioelectronics　31　(2)　77-80　2020/05

    Publisher: 応用物理学会有機分子・バイオエレクトロニクス分科会

    ISSN： 2423-8805

  4. Engineering the Structure and Function of Cultured Neuronal Networks Using Microfabricated Substrates for Understanding and Exploiting Multicellular Computation Invited Peer-reviewed

    Hideaki Yamamoto, Ayumi Hirano-Iwata

    J102-C　(12)　340-347　2019/12

  5. Evaluation of Reservoir-Based Predictive Coding in Cultured Neurons with Spiking Neural Network Model

    Ishikawa Yoshitaka, Shinkawa Takumi, Sumi Takuma, Kato Hideyuki, Yamamoto Hideaki, Katori Yuichi

    IEICE Proceeding Series　76　693-696　2023/09/21

    Publisher: The Institute of Electronics, Information and Communication Engineers

    DOI： 10.34385/proc.76.d2l-14 　

    eISSN： 2188-5079

    More details Close

    The field of physical reservoir computing, an approach that leverages the dynamics of physical systems for information processing, continues to evolve. Utilizing cultured neurons as a physical system in physical reservoir computing has helped to elucidate the relationship between the structure of neuronal networks and information processing. In this study, we present a model that integrates predictive coding and reservoir computing in cultured neurons, serving as a sensory information processing model. We demonstrate that the relationship between the structure of neuronal networks and the number of neurons influences sensory information processing. This study may provide a foundation for further investigations into information processing in biological neuronal networks.

  6. Spiking and Bursting Properties of Spontaneous Activities in Modular Spiking Neuronal Networks

    Kato Hideyuki, Shinkawa Takumi, Ishikawa Yoshitaka, Sumi Takuma, Yamamoto Hideaki, Katori Yuichi

    IEICE Proceeding Series　76　601-601　2023/09/21

    Publisher: The Institute of Electronics, Information and Communication Engineers

    DOI： 10.34385/proc.76.c4l-15 　

    eISSN： 2188-5079

    More details Close

    The application of cultured neuronal networks as a physical reservoir is one of the attractive topics in reservoir computing. One can easily imagine that the performance of reservoir computing depends on their network topology because the topology influences neuronal activities in the network. In contrast, neurons try to sustain their activities as possible. This property is called the homeostasis of neurons. In this study, we mathematically model the networks with synaptically connected spiking neurons by considering the above two aspects and then evaluate spontaneous neuronal activities from the viewpoints of spiking and bursting behaviors.

  7. Development of a hERG channel sensor for evaluation of drug side effects

    渡辺恭也, 横田澪央, 加藤美生, 佐藤まどか, 但木大介, 小宮麻希, 山本英明, 井上遥香, 戸澤譲, 庭野道夫, 平野愛弓

    電子情報通信学会技術研究報告　119　(431(CPM2019 91-109))　2020

    ISSN： 0913-5685

  8. Quantitative Analysis of Dynamical Complexity in Cultured Neuronal Network Models for Reservoir Computing Applications

    Satoshi Moriya, Hideaki Yamamoto, Ayumi Hirano-Iwata, Shigeru Kubota, Shigeo Sato

    Proceedings of the International Joint Conference on Neural Networks　2019-July　2019/07/01

    Publisher: IEEE

    DOI： 10.1109/IJCNN.2019.8852207 　

  9. Construction of an artificial cell membrane device and its application to a sensor for drug side effects

    119　(9)　23-26　2019/04/18

    Publisher: 電子情報通信学会

    ISSN： 0913-5685

  10. Quantitative assessment of drug side effect on hERG channels based on artificial bilayer lipid membranes and cell-free expression system

    横田澪央, 加藤美生, 山浦大地, 常田悠介, 佐藤まどか, 但木大介, 小宮麻希, 山本英明, 井上遥, 戸澤譲, 庭野道夫, 平野愛弓

    電子情報通信学会技術研究報告　118　(461(CPM2018 101-120))　2019

    ISSN： 0913-5685

  11. 人工細胞膜に基づいたイオンチャネルタンパク質の新規解析系の開発

    小宮麻希, 横田澪央, 常田悠介, 佐藤まどか, 加藤美生, 山浦大地, 但木大介, 馬騰, 山本英明, 庭野道夫, 平野愛弓, 平野愛弓

    日本分析化学会年会講演要旨集(Web)　68th　2019

  12. Stable formation of an artificial cell membrane sensor and its application to sensing drug side effects

    118　(9)　37-40　2018/04/19

    Publisher: 電子情報通信学会

    ISSN： 0913-5685

  13. 微細加工チップ中に形成した脂質二分子膜への無細胞合成イオンチャネルの包埋と薬物副作用評価の定量

    横田澪央, 加藤美生, 吉田美優, 山浦大地, 荒田航平, 但木大介, 山本英明, 戸澤譲, 庭野道夫, 平野愛弓, 平野愛弓

    日本分析化学会年会講演要旨集　67th　2018

  14. 微細加工チップ中に形成した脂質二分子膜への無細胞合成イオンチャネルの包埋と薬物副作用の定量

    加藤美生, 吉田美優, 山浦大地, 荒田航平, 但木大介, 山本英明, 戸澤譲, 庭野道夫, 平野愛弓, 平野愛弓

    応用物理学会春季学術講演会講演予稿集(CD-ROM)　65th　2018

  15. 微細加工シリコンチップ中に再構成した脂質二分子膜における無細胞合成hERGチャネルの薬物副作用の定量

    常田悠介, 加藤美生, 山浦大地, 荒田航平, 但木大介, 小宮麻希, 山本英明, 井上遥香, 戸澤譲, 庭野道夫, 平野愛弓, 平野愛弓

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)　79th　2018

  16. Variation in spontaneous activity patterns in modular neuronal network models

    116　(521)　133-136　2017/03/13

    Publisher: 電子情報通信学会

    ISSN： 0913-5685

  17. 脂質二分子膜に対する多方向電気特性評価用シリコンチップの作製

    大堀健, 平野愛弓, 平野愛弓, 但木大介, 山浦大地, 荒木駿, 吉田美優, 荒田航平, 山本英明, 庭野道夫

    応用物理学会春季学術講演会講演予稿集(CD-ROM)　64th　2017

  18. 半導体シリコンチップ上に作製された微細孔における安定な人工脂質二分子膜形成のためのエッジ構造の最適化

    但木大介, 平野愛弓, 平野愛弓, 山浦大地, 荒木駿, 吉田美優, 荒田航平, 大堀健, 山本英明, 庭野道夫

    応用物理学会春季学術講演会講演予稿集(CD-ROM)　64th　2017

  19. マルチウェル型チャンバーによる高効率薬物副作用検査系の構築

    山浦大地, 宮田隆典, 荒木駿, 但木大介, 山本英明, 平野愛弓, 平野愛弓

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)　78th　2017

  20. 支持体表面修飾による自立型脂質二重膜の安定性向上

    山浦大地, 但木大介, 荒木駿, 吉田美優, 荒田航平, 大堀健, 山本英明, 荻野俊郎, 庭野道夫, 平野愛弓, 平野愛弓

    応用物理学会春季学術講演会講演予稿集(CD-ROM)　64th　2017

  21. 微細加工シリコンチップにおける脂質二分子膜中のイオンチャネル電流計測

    荒田航平, 平野愛弓, 平野愛弓, 但木大介, 山浦大地, 荒木駿, 吉田美優, 大堀健, 山本英明, 庭野道夫

    応用物理学会春季学術講演会講演予稿集(CD-ROM)　64th　2017

  22. Investigation of network structure and synchronous activity in modularly-structured neuronal network models

    116　(313)　33-38　2016/11/18

    Publisher: 電子情報通信学会

    ISSN： 0913-5685

  23. Modification of Cell-Substrate Interface using TiO2 Photocatalysis

    Yamamoto H, Hirano-Iwata A, Tanii T, Niwano M

    J. Surf. Sci. Soc. Jpn.　37　(5)　224-229　2016/05

    DOI： 10.1380/jsssj.37.224 　

  24. Integration of surface science and neurobiology for designing neuronal networks and investigating their structure-function relationships

    1-6　2016

    Publisher: 旭硝子財団

    ISSN： 1882-0069

  25. ナノテーパー構造に基づく人工細胞膜の構築

    荒木駿, 平野愛弓, 但木大介, 石橋健一, 山本英明, 庭野道夫, 庭野道夫

    日本表面科学会東北・北海道支部講演会講演予稿集　2015　2016

  26. 微細加工シリコンチップにおけるナノエッジ構造と人工脂質二分子膜形成

    荒田航平, 荒田航平, 平野愛弓, 平野愛弓, 石橋健一, 石橋健一, 但木大介, 但木大介, 荒木駿, 荒木駿, 吉田美優, 吉田美優, 山本英明, 山本英明, 庭野道夫, 庭野道夫, 庭野道夫

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)　77th　2016

  27. 人工脂質二分子膜形成のための微細孔を有する半導体シリコンチップの作製プロセスの改善

    但木大介, 平野愛弓, 石橋健一, 荒木駿, 吉田美優, 荒田航平, 大堀健, 山本英明, 庭野道夫

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)　77th　2016

  28. 半導体微細加工を用いた人工細胞膜アレイの開発

    荒木駿, 平野愛弓, 宮田隆典, 但木大介, 石橋健一, 山本英明, 庭野道夫, 庭野道夫

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)　77th　2016

  29. 半導体微細加工を用いた人工細胞膜アレイの開発

    荒木駿, 平野愛弓, 宮田隆典, 但木大介, 石橋健一, 山本英明, 庭野道夫, 庭野道夫

    真空に関する連合講演会講演予稿集　57th　2016

  30. 微細加工シリコン基板に基づく脂質二分子膜へのヒトイオンチャネル機能の再構成

    吉田美優, 平野愛弓, 山本英明, 山本英明, 但木大介, 庭野道夫, 庭野道夫

    真空に関する連合講演会講演予稿集　57th　2016

  31. CI-3-6 Functional Reconstitution of Ion Channel Proteins in Microfabricated Silicon Chips

    Hirano-Iwata Ayumi, Yamamoto Hideaki, Kimura Yasuo, Niwano Michio

    Proceedings of the Society Conference of IEICE　2015　(2)　"SS-52"-"SS-53"　2015/08/25

    Publisher: The Institute of Electronics, Information and Communication Engineers

  32. Nano-scale patterning of functional molecules for biomolecule detection and cell manipulation

    Yamamoto H, Tanii T

    Molecular Electronics and Bioelectronics　24　(4)　266-271　2013/11

  33. Fabrication of Artificial Neuronal Circuits by in situ Modification of Cell-Culture Scaffolds by Photocatalytic Activity of Titanium Dioxide and Dynamics Analyses of Them

    (33)　123-129　2012

    Publisher: 日本板硝子材料工学助成会

    ISSN： 1883-3128

  34. 液中レーザプロセスによる細胞培養環境場での神経突起誘導

    山本英明, 山本英明, 岡野和宣, 岡野和宣, 谷井孝至, 谷井孝至, 出村崇徳, 細川陽一郎, 増原宏, 中村俊

    応用物理学関係連合講演会講演予稿集(CD-ROM)　58th　2011

  35. 液中レーザープロセスによる細胞培養環境場での神経突起誘導とその機構

    山本英明, 山本英明, 岡野和宣, 岡野和宣, 出村崇徳, 細川陽一郎, 谷井孝至, 谷井孝至, 中村俊

    応用物理学会学術講演会講演予稿集(CD-ROM)　72nd　2011

  36. In situ guidance of individual neurites by wet femtosecond-laser processing of organosilane monolayers

    Hideaki Yamamoto, Kazunori Okano, Takashi Tanii, Takanori Demura, Yoichiroh Hosokawa, Shun Nakamura

    NEUROSCIENCE RESEARCH　71　E236-E237　2011

    DOI： 10.1016/j.neures.2011.07.1033 　

    ISSN： 0168-0102

  37. Atomistic Modeling of GeO_2/Ge Interface Structure by Molecular Dynamics : Comparison with SiO_2/Si Interface

    WATANABE Takanobu, ONDA Tomoya, TOSAKA Ryo, YAMAMOTO Hideaki

    IEICE technical report　109　(87)　3-8　2009/06/12

    Publisher: The Institute of Electronics, Information and Communication Engineers

    ISSN： 0913-5685

    More details Close

    We have performed atomistic modeling of GeO_2/Ge interface structure by using newly developed interatomic force-field for Ge, O mixed systems. The force-field for Ge, O systems is designed by modifying an existing potential function for Si, O systems which is developed in our previous work. The binding energies and distortion energies of bond angles in the Ge, O system is smaller than corresponding bonds and angles in the Si, O system as a whole, but only a Ge-O-Ge bridging oxygen angle is found to be harder than a Si-O-Si angle. Using these force-fields, we have modeled GeO_2/Ge and SiO_2/Si interface structures to investigate stress distribution, bond angle distribution, and defect density within the two oxide films. The present results show that the oxidation-induced strain is weaker in the GeO_2 film than in the SiO_2 film, and the defect density at the GO_2/Ge interface is lower than that at the SiO_2/Si interface.

Show all ︎Show first 5

Books and Other Publications 2

  1. 『新しい途を拓く』（東北大学教養教育院叢書「大学と教養」第8巻）

    東北大学教養教育院

    東北大学出版会　2025/03

    ISBN: 9784861634062

  2. 百科繚覧 Vol. 2: 若手研究者が挑む学際フロンティア

    東北大学学際科学フロンティア研究所「百科繚覧」編集委員会

    東北大学出版会　2020/01

    ISBN: 9784861633133

Presentations 223

  1. 距離依存結合構造を有するアナログSNN回路を用いたリザバー計算による音声信号分類の高性能化

    酒井哲汰, 守谷 哲, 石川将也, 山本英明, 佐藤茂雄

    2025年電子情報通信学会総合大会　2025/03/27

  2. 低電力リザバー計算システムの構築に向けた スパイキングニューラルネットワークLSIの設計

    石川将也, 守谷 哲, 酒井哲汰, 山本英明, 佐藤茂雄

    2025年電子情報通信学会総合大会　2025/03/27

  3. 低消費電力アナログCMOS回路を用いたスパイキングニューラルネットワークの実現とリザバー計算応用 Invited

    佐藤茂雄, 守谷 哲, 石川将也, 山本英明

    令和7年電気学会全国大会　2025/03/20

  4. Magnetic µCoil fibers for non-invasive neuromodulation

    E. Lebourdonnec, K. Hiramoto, H. Yamamoto, A. Hirano-Iwata, S. Ohnuma, H. Masumoto, Y. Guo

    2025/03/17

  5. マイクロ流体デバイスで培養した神経細胞の高倍率イメージング

    齋藤 新, 山本英明, 室田白馬, 佐藤茂雄, 平野愛弓

    第72回応用物理学会春季学術講演会　2025/03/15

  6. 薄膜型マイクロ流体デバイスを用いたヒトiPS細胞由来大脳皮質神経細胞のパターン培養

    酒井原一守, 山本英明, 西村周泰, 遠藤壮太, 金野智浩, 佐藤茂雄, 正水芳人, 平野愛弓

    第72回応用物理学会春季学術講演会　2025/03/15

  7. 「ニューラルネットワーク」の多様性：ANN, SNN, そしてBNN Invited

    山本英明

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2025/03/06

  8. Induction of neuronal ensemble plasticity by optogenetic stimulation in micropatterned neuronal networks

    H. Murota, H. Yamamoto, N. Monma, S. Sato, A. Hirano-Iwata

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  9. Exploring the functional role of directional intermodular coupling through in vitro experiments and in silico modeling

    N. Monma, H. Yamamoto, N. Fujiwara, H. Murota, S. Moriya, A. Hirano-Iwata, S. Sato

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  10. Enhanced chronic response to interleukin-6 in micropatterned cortical networks

    M. Sakaibara, H. Yamamoto, H. Murota, S. Sato, A. Hirano-Iwata

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  11. In-silico and in-vitro modeling of reservoir-based predictive coding in biological neuronal networks on multielectrode arrays

    Y. Sato, H. Yamamoto, Y. Ishikawa, T. Sumi, Y. Sono, S. Sato, Y. Katori, A. Hirano-Iwata

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  12. Exploring reservoir computing in neuronal dynamical systems

    T. Sumi, H. Yamamoto, Y. Katori, A. M. Houben, H. Kato, J. Soriano, A. Hirano-Iwata, H. Chiba

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  13. Synaptic selection rule for single neurons in an autaptic culture with STDP

    R. Onda, M. Yamazoe, K. Oishi, Y. Kuwabara, M. Ishida, H. Yamamoto, T. Tanii

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  14. In vitro & in vivo evaluation of directional functional connectivity by anatomical connectivity

    K. Kawamoto, N. Monma, R. Li, H. Yamamoto, T. Matsui

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  15. Study on the impact of connectivity and spontaneous activity on the e-prop training algorithm

    D. Noè, H. Yamamoto, Y. Katori, S. Sato

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  16. Evaluation of the effect of calcium ion concentration on reservoir computing using cultured neuron model

    S. Tanaka, Y. Ishikawa, H. Kato, H. Yamamoto, Y. Katori

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  17. Ten-class speech classification under noisy conditions via predictive coding and reservoir computing in spiking neural networks

    Y. Ishikawa, Y. Sato, T. Sumi, H. Kato, H. Yamamoto, Y. Katori

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  18. The re-launch of "Multicellular Neurobiocomputing" and the role of neuroengineering technologies Invited

    H. Yamamoto, K. Watanabe, T. Sumi, S. Sato, A. Hirano-Iwata

    The Future of Computing: Perspectives (The 13th RIEC International Symposium on Brain Functions and Brain Computer)　2025/02/27

  19. Analog circuit implementation of;pike;imi;lasticity for;efficient computing

    S. Moriya, Y. Iida, H. Yamamoto, S. Sato

    2024 International Symposium on Nonlinear Theory and Its Applications (NOLTA2024)　2024/12/05

  20. Role of synaptic plasticity and modular structure on neuronal network recovery from injury

    T. Sumi, A. Houben, H. Yamamoto, H. Kato, Y. Katori, H. Chiba, J. Soriano, A. Hirano-Iwata

    2024 International Symposium on Nonlinear Theory and Its Applications (NOLTA2024)　2024/12/05

  21. Noise reduction in speech recognition using predictive coding and reservoir computing in cultured neuronal networks in spiking neural networks

    Y. Ishikawa, Y. Sato, T. Sumi, H. Kato, H. Yamamoto, Y. Katori

    2024 International Symposium on Nonlinear Theory and Its Applications (NOLTA2024)　2024/12/05

  22. K. Kawamoto, N. Monma, Y. Kobayashi, K. Hayashi, H. Yamamoto, T. Matsui

    K. Kawamoto, N. Monma, Y. Kobayashi, K. Hayashi, H. Yamamoto, T. Matsui

    2024 International Symposium on Nonlinear Theory and Its Applications (NOLTA2024)　2024/12/05

  23. Neuroengineering technologies for reconstituting neuronal network functions in vitro

    H. Yamamoto, T. Sumi, A. Hirano-Iwata, S. Sato

    2024 International Symposium on Nonlinear Theory and Its Applications (NOLTA2024)　2024/12/05

  24. Fabrication of microfluidic devices for reconstituting brain region connectivity with cultured neurons

    S. Endo, H. Yamamoto, K. Nishimura, M. Sakaibara, N. Monma, Y. Masamizu, A. Hirano-Iwata, S. Sato

    17th International Symposium on Nanomedicine (ISNM2024)

  25. Microelectronic fiber for bio-signal recording and stimulation

    E. le Bourdonnec, K. Hiramoto, H. Yamamoto, A. Hirano-Iwata, S. Ohnuma, H. Masumoto, Y. Guo

    Wearable and Implantable Sensors and Electrochemical Devices (WISED) 2024

  26. 培養細胞における神経発火パターンの可塑性制御技術の開発

    室田白馬, 山本英明, 佐藤茂雄, 平野愛弓

    化学とマイクロ・ナノシステム学会 第50回研究会　2024/11

  27. Cell engineering technologies to regulate neuronal networks Invited

    H. Yamamoto, A. Hirano-Iwata, S. Sato

    37th International Microprocesses and Nanotechnology Conference (MNC 2024)　2024/11/14

  28. マイクロ流体デバイスを活用した神経回路機能のボトムアップ解析 Invited

    山本英明

    第14回分子サイバネティクス・第58回分子ロボティクス定例研究会　2024/11/08

  29. In silico modeling of reservoir-based predictive coding in biological neuronal networks on multielectrode arrays

    Y. Sato, H. Yamamoto, Y. Ishikawa, T. Sumi, Y. Sono, S. Sato, Y. Katori, A. Hirano-Iwata

    4th International Symposium on Design & Engineering by Joint Inverse Innovation for Materials Architecture (DEJI2MA2024)　2024/10/03

  30. マルコフ連鎖モデルによる神経細胞ネットワークの構造機能相関解析

    門間信明, 山本英明, 藤原直哉, 佐藤茂雄

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2024/09/28

  31. STDP則のアナログCMOS回路実装と分類タスクへの応用

    飯田陽介, 守谷 哲, 山本英明, 佐藤茂雄

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2024/09/27

  32. In vitro neuroengineering using microfluidic devices Invited

    H. Yamamoto, A. Hirano-Iwata, S. Sato

    11th Asian Conference on Nanoscience and Nanotechnology (AsiaNANO2024)　2024/09/23

  33. レーザーマイクロダイセクションによるマイクロパターン培養神経回路への局所損傷

    渡邉啓太, 山本英明, 室田白馬, 住 拓磨, 佐藤茂雄, 平野愛弓

    第85回応用物理学会秋季学術講演会　2024/09/20

  34. 高密度微小電極アレイを用いた培養神経回路における集団ダイナミクスのフィードバック制御と時系列信号生成タスクによる性能評価

    薗 勇輝, 山本英明, 佐藤有弥, 西 悠聖, 住 拓磨, 香取勇一, 平野愛弓, 佐藤茂雄

    第39回ライフサポート学会大会(LIFE 2024)　2024/09/14

  35. モジュール構造型培養神経回路の物理リザバーコンピューティング応用

    住 拓磨, 山本英明, 千葉逸人, 香取勇一, 平野愛弓

    第39回ライフサポート学会大会(LIFE 2024)　2024/09/13

  36. 培養神経回路モデルにおけるリザバー計算を用いた予測符号化による感覚信号処理のノイズ削減

    石川慶孝, 佐藤有弥, 住 拓磨, 加藤秀行, 山本英明, 香取勇一

    第34回日本神経回路学会全国大会　2024/09/12

  37. 高密度微小電極アレイを用いた培養神経細胞回路の集団ダイナミクスのフィードバック制御

    薗 勇輝, 山本英明, 佐藤有弥, 西 悠聖, 住 拓磨, 香取勇一, 平野愛弓, 佐藤茂雄

    第34回日本神経回路学会全国大会　2024/09/11

  38. Structure and function in biological neuronal networks: Engineering approaches Invited

    H. Yamamoto

    International Workshop on Complex Systems and Their Interdisciplinary Applications　2024/08/06

  39. 高密度多点電極アレイ上のマイクロパターン培養神経回路を用いた物理リザバーコンピューティング

    西 悠聖, 山本英明, 住 拓磨, 薗 勇輝, 佐藤有弥, 香取勇一, 平野愛弓, 佐藤茂雄

    第47回日本神経科学大会 (NEURO2024)　2024/07/24

  40. Design of mixed-signal LSI with analog spiking neural network and digital inference circuits for reservoir computing

    S. Moriya, H. Yamamoto, M. Ishikawa, Y. Yuminaka, Y. Horio, J. Madrenas, S. Sato

    IEEE World Congress on Computational Intelligence (WCCI) 2024　2024/07/04

  41. Dynamical complexity in engineered biological neuronal networks with directional and modular connections

    N. Monma, H. Yamamoto, H. Murota, S. Moriya, A. Hirano-Iwata, S. Sato

    FENS Forum 2024　2024/06/28

  42. Effect of optogenetic modulation of neuronal ensembles in cultured neuronal networks

    H. Murota, H. Yamamoto, N. Monma, S. Sato, A. Hirano-Iwata

    FENS Forum 2024　2024/06/28

  43. Reservoir computing using cultured neuronal networks with modular topology

    T. Sumi, H. Yamamoto, Y. Katori, K. Ito, H. Kato, H. Chiba, S. Sato, A. Hirano-Iwata

    FENS Forum 2024　2024/06/28

  44. Biological reservoir computing: Multicellular computing within biological neuronal networks Invited

    H. Yamamoto

    FENS 2024 Satellite Event "Cortical Neurons for Biological Computing"　2024/06/24

  45. In silico modeling of reservoir-based predictive coding in biological neuronal networks on multielectrode arrays

    Y. Sato, H. Yamamoto, Y. Ishikawa, T. Sumi, Y. Sono, S. Sato, Y. Katori, A. Hirano-Iwata

    11th International Conference on Molecular Electronics & Bioelectronics (M&BE11)

  46. Using engineering tools to study the brain Invited

    H. Yamamoto

    UPC Roundtable "Meeting Biological and Electronic Neurons for Biohybrid and In-Vitro Neural Networks: Challenges and Applications"　2024/06/17

  47. Microcontact printing for patterning neurons and neuronal networks International-presentation

    H. Yamamoto, K. Ide, R. Matsumura, K. Wakimura, M. Niwano, A. Hirano-Iwata

    11th International Symposium on Nanomedicine (ISNM2017)　2017/12/13

  48. Spontaneous activity patterns of micropatterned cortical neurons: Dependence on the number of modules International-presentation

    K. Ide, H. Yamamoto, K. Wakimura, M. Niwano, A. Hirano-Iwata

    11th International Symposium on Nanomedicine (ISNM2017)　2017/12/13

  49. Modified aperture surface with self-assembled monolayer enhanced stability of suspended lipid membrane International-presentation

    D. Yamaura, D. Tadaki, H. Yamamoto, M. Niwano, A. Hirano-Iwata

    11th International Symposium on Nanomedicine (ISNM2017)　2017/12/13

  50. モジュール構造型神経回路モデルにおける同期活動のメカニズム

    守谷 哲, 山本英明, 井手克哉, 秋間学尚, 平野愛弓, 庭野道夫, 久保田 繁, 佐藤茂雄

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2017/11/24

  51. Engineering modular organization of living neuronal networks by microcontact printed proteins International-presentation

    H. Yamamoto, K. Ide, K. Wakimura, T. Tanii, M. Niwano, A. Hirano-Iwata

    The 8th International Symposium on Surface Science (ISSS-8)　2017/10/22

  52. Microfabrication method for investigating ion channel activities International-presentation

    A. Hirano-Iwata, D. Tadaki, D. Yamaura, K. Arata, T. Ohori, T. Ma, H. Yamamoto, M. Niwano

    The 8th International Symposium on Surface Science (ISSS-8)　2017/10/22

  53. Generation of nanobubbles by a porous alumina thin film with ordered straight nanoholes International-presentation

    T. Ma, H. Yamamoto, Y. Kimura, A. Hirano-Iwata, M. Niwano

    The 8th International Symposium on Surface Science (ISSS-8)　2017/10/22

  54. Functional reconstruction of cell-free synthesized human ion channels in artificial bilayer lipid membranes International-presentation

    K. Arata, D. Tadaki, H. Yamamoto, Y. Tozawa, M. Niwano, A. Hirano-Iwata

    The 8th International Symposium on Surface Science (ISSS-8)　2017/10/22

  55. Microfabricated silicon chips for evaluation of multidirectional electrical properties of BLMs International-presentation

    T. Ohori, D. Tadaki, H. Yamamoto, M. Niwano, A. Hirano-Iwata

    The 8th International Symposium on Surface Science (ISSS-8)　2017/10/22

  56. An experimental and computaitonal study on spontaneous firing pattern of a single neuron

    K. Hattori, S. Kono, T. Yoneyama, A. Nakanishi, H. Yamamoto, T. Tanii

    第27回日本神経回路学会全国大会　2017/09/20

  57. Frequency-response curves of micropatterned hippocampal neurons: Effect of cell morphology on membrane impedance International-presentation

    R. Matsumura, H. Yamamoto, S. Katsurabayashi, M. Niwano, A. Hirano-Iwata

    2017 International Conference on Solid State Devices and Materials (SSDM2017)　2017/09/19

  58. マイクロコンタクトプリンティング法による生体分子パターニングと培養神経回路の構造制御

    山本英明, 松村亮佑, 井手克哉, 早川岳志, 脇村 桂, 庭野道夫, 平野愛弓

    第78回応用物理学会秋季学術講演会　2017/09/05

  59. 生体分子パターンの形状による神経細胞回路のモジュール間相互作用の制御

    井手克哉, 山本英明, 守谷 哲, 脇村 桂, 谷井孝至, 秋間学尚, 久保田 繁, 佐藤茂雄, 庭野道夫, 平野愛弓

    第78回応用物理学会秋季学術講演会　2017/09/05

  60. 接続構造を制御した神経細胞回路ユニットの入出力特性

    早川岳志, 山本英明, 松村亮佑, 平野愛弓

    第78回応用物理学会秋季学術講演会　2017/09/05

  61. 可視光応答酸化チタン薄膜の光触媒能を利用した細胞の液中パターニング(II)

    河野 翔, 黒飛 敦, 服部晃平, 山本英明, 谷井孝至

    第78回応用物理学会秋季学術講演会　2017/09/05

  62. マルチウェル型チャンバーによる高効率薬物副作用検査系の構築

    山浦大地, 宮田隆典, 荒木 駿, 但木大介, 山本英明, 平野愛弓

    第78回応用物理学会秋季学術講演会　2017/09/05

  63. ナノ・マイクロ構造制御に基づく人工細胞膜の安定化

    平野愛弓, 但木大介, 大堀 健, 荒田航平, 馬 騰, 山本英明, 庭野道夫

    第37回表面科学学術講演大会　2017/08/17

  64. 可視光応答酸化チタンの光触媒作用を活用した液中表面改質と細胞パターニング

    河野 翔, 黒飛 敦, 服部晃平, 山本英明, 平野愛弓, 谷井孝至

    第37回表面科学学術講演大会　2017/08/17

  65. Frequency characteristics of morphology controlled neurons on microcontact printed protein patterns International-presentation

    R. Matsumura, H. Yamamoto, S. Katsurabayashi, M. Niwano, A. Hirano-Iwata

    9th International Conference on Molecular Electronics and Bioelectronics (M&BE9)　2017/06/26

  66. Fabrication of multidirectional electrical characteristics evaluation system of BLM based on microfabrication International-presentation

    T. Ohori, D. Tadaki, H. Yamamoto, M. Niwano, A. Hirano-Iwata

    9th International Conference on Molecular Electronics and Bioelectronics (M&BE9)　2017/06/26

  67. Lipophobic surfaces enhance the stability of suspended lipid bilayers International-presentation

    D. Yamaura, D. Tadaki, H. Yamamoto, M. Niwano, A. Hirano-Iwata

    9th International Conference on Molecular Electronics and Bioelectronics (M&BE9)　2017/06/26

  68. Recording activities of cell-free synthesized ion-channels at artificial lipid bilayers formed in microfabricated silicon chips International-presentation

    K. Arata, D. Tadaki, H. Yamamoto, Y. Tozawa, M. Niwano, A. Hirano-Iwata

    9th International Conference on Molecular Electronics and Bioelectronics (M&BE9)　2017/06/26

  69. TiO2-assisted photocatalytic lithography: Applications in patterning neuronal cells and networks International-presentation

    H. Yamamoto, S. Kono, S. Fujishiro, K. Furusawa, T. Tanii, M. Niwano, A. Hirano-Iwata

    European Materials Research Society (E-MRS) 2017 Spring Meeting　2017/05/22

  70. Modularity-dependent modulation of synchronized bursting activity in cultured neuronal network models International-presentation

    S. Moriya, H. Yamamoto, H. Akima, A. Hirano-Iwata, M. Niwano, S. Kubota, S. Sato

    International Joint Conference on Neural Networks (IJCNN) 2017　2017/05/14

  71. 半導体微細加工に基づく薬物副作用センサの開発

    平野愛弓, 但木大介, 山浦大地, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫

    電子情報通信学会 電子デバイス(ED）研究会　2017/04/20

  72. 自己組織化単分子膜による自立型脂質二分子膜の膜特性制御

    山浦大地, 但木大介, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫, 平野愛弓

    電子情報通信学会 電子デバイス(ED）研究会　2017/04/20

  73. 安定な人工脂質二分子膜形成のためのテーパー構造を有した微細孔をもつ半導体シリコンチップの作製

    但木大介, 山浦大地, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫, 平野愛弓

    電子情報通信学会 電子デバイス(ED）研究会　2017/04/20

  74. モジュール構造型培養神経回路の計算論的モデリング

    山本英明, 守谷 哲, 井手克哉, 松村亮佑, 秋間学尚, 久保田 繁, 谷井孝至, 佐藤茂雄, 庭野道夫, 平野愛弓

    第64回応用物理学会春季学術講演会　2017/03/14

  75. 可視光応答酸化チタン薄膜の光触媒能を利用した細胞の液中パターニング

    古澤昂平, 河野 翔, 藤城翔偉, 山本英明, 谷井孝至

    第64回応用物理学会春季学術講演会　2017/03/14

  76. 支持体表面修飾による自立型脂質二重膜の安定性向上

    山浦大地, 但木大介, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 荻野俊郎, 庭野道夫, 平野愛弓

    第64回応用物理学会春季学術講演会　2017/03/14

  77. 脂質二分子膜に対する多方向電気特性評価用シリコンチップの作製

    大堀 健, 平野愛弓, 但木大介, 山浦大地, 荒木 駿, 吉田美優, 荒田航平, 山本英明, 庭野道夫

    第64回応用物理学会春季学術講演会　2017/03/14

  78. マイクロ加工表面を用いた培養神経回路のモジュール性制御

    井手克哉, 山本英明, 松村亮佑, 谷井孝至, 吉信達夫, 庭野道夫, 平野愛弓

    第64回応用物理学会春季学術講演会　2017/03/14

  79. マイクロ加工表面を用いた単一神経細胞の周波数特性制御

    松村亮佑, 山本英明, 桂林秀太郎, 庭野道夫, 平野愛弓

    第64回応用物理学会春季学術講演会　2017/03/14

  80. Functional integration of human ion channels in artificially formed bilayer lipid membranes

    A. Hirano-Iwata, D. Tadaki, D. Yamaura, S. Araki, M. Yoshida, K. Arata, T. Ohori, H. Yamamoto, M. Niwano

    第64回応用物理学会春季学術講演会　2017/03/14

  81. 半導体シリコンチップ上に作製された微細孔における安定な人工脂質二分子膜形成のためのエッジ構造の最適化

    但木大介, 平野愛弓, 山浦大地, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫

    第64回応用物理学会春季学術講演会　2017/03/14

  82. 微細加工シリコンチップにおける脂質二分子膜中のイオンチャネル電流計測

    荒田航平, 平野愛弓, 但木大介, 山浦大地, 荒木 駿, 吉田美優, 大堀 健, 山本英明, 庭野 道夫

    第64回応用物理学会春季学術講演会　2017/03/14

  83. モジュール構造型神経回路モデルにおける自発活動パターンのばらつきに関する考察

    守谷 哲, 山本英明, 井手克哉, 秋間学尚, 平野愛弓, 庭野道夫, 久保田繁, 佐藤茂雄

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2017/03/13

  84. Analyzing synchronized bursts in cortical neuronal networks International-presentation

    S. Moriya, H. Yamamoto, H. Akima, A. Hirano-Iwata, M. Niwano, S. Kubota, S. Sato

    5th RIEC International Symposium on Brain Functions and Brain Computer　2017/02/27

  85. A computational study on spontaneous activity of a single neuron International-presentation

    K. Hattori, S. Kono, H. Yamamoto, T. Tanii

    5th RIEC International Symposium on Brain Functions and Brain Computer　2017/02/27

  86. Unidirectional signal propagation in primary neurons micropatterned at a single-cell resolution International-presentation

    R. Matsumura, H. Yamamoto, S. Katsurabayashi, M. Niwano, A. Hirano-Iwata

    5th RIEC International Symposium on Brain Functions and Brain Computer　2017/02/27

  87. In situ modification of cell-culture scaffolds by photocatalysis of visible-light-responsible TiO2 film International-presentation

    S. Kono, K. Furusawa, S. Fujishiro, H. Yamamoto, T. Tanii

    5th RIEC International Symposium on Brain Functions and Brain Computer　2017/02/27

  88. Controlling modularity of cortical neuronal networks using micropatterned surfaces International-presentation

    K. Ide, H. Yamamoto, R. Matsumura, T. Tanii, M. Niwano, A. Hirano-Iwata

    5th RIEC International Symposium on Brain Functions and Brain Computer　2017/02/27

  89. Synchronization in neuronal networks with modular geometry International-presentation

    H. Yamamoto, T. Tanii, S. Kubota, S. Sato, M. Niwano, A. Hirano-Iwata

    5th RIEC International Symposium on Brain Functions and Brain Computer　2017/02/27

  90. 自己組織化単分子膜修飾による自立型脂質二分子膜の安定性の制御

    山浦大地, 但木大介, 荒木 駿, 吉田美優, 荒田航平, 山本英明, 荻野俊郎, 庭野道夫, 平野愛弓

    2016年真空・表面科学合同講演会　2016/11/29

  91. 微細加工シリコン基板に基づく脂質二分子膜へのヒトイオンチャネル機能の再構成

    吉田美優, 平野愛弓, 山本英明, 但木大介, 庭野道夫

    2016年真空・表面科学合同講演会　2016/11/29

  92. Structure-function relationships in living neuronal networks: a surface engineering approach International-presentation

    H. Yamamoto, A. Hirano-Iwata, T. Tanii, M. Niwano

    10th Anniversary International Symposium on Nanomedicine (ISNM2016)　2016/11/24

  93. モジュール型神経回路モデルにおける構造と同期活動の解析

    守谷 哲, 山本英明, 秋間学尚, 平野愛弓, 庭野道夫, 久保田繁, 佐藤茂雄

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2016/11/18

  94. Reconstitution of human ion channels into solvent-free lipid bilayers in microfabricated silicon chips: accelerated vesicle fusion via centrifugation International-presentation

    A. Hirano-Iwata, M. Yoshida, S. Araki, D. Tadaki, R. Miyata, K. Ishibashi, H. Yamamoto, M. Niwano

    International Conference on Solid State Devices and Materials (SSDM) 2016　2016/09/27

  95. Equivalent circuit simulation of a neuron-electrode interface: mechanism of signal amplification by resistive covering International-presentation

    R. Matsumura, H. Yamamoto, A. Hirano-Iwata, M. Niwano

    International Conference on Solid State Devices and Materials (SSDM) 2016　2016/09/27

  96. マイクロ加工表面を用いた神経信号伝達の整流性制御

    松村亮佑, 山本英明, 桂林秀太郎, 平野愛弓, 庭野道夫

    第77回応用物理学会秋季学術講演会　2016/09/13

  97. 有機単分子膜パターンにより細胞間接続を制御した2細胞神経回路の構築と解析

    河野 翔, 藤森壮也, 山本英明, 谷井孝至

    第77回応用物理学会秋季学術講演会　2016/09/13

  98. Reconstitution of human ion channels in lipid bilayers formed in microfabricated apertures

    A. Hirano-Iwata, M. Yoshida, S. Araki, D. Tadaki, R. Miyata, K. Ishibashi, K. Arata, T. Ohori, H. Yamamoto, M. Niwano

    第77回応用物理学会秋季学術講演会　2016/09/13

  99. 微細加工シリコンチップにおけるナノエッジ構造と人工脂質二分子膜形成

    荒田航平, 平野愛弓, 石橋健一, 但木大介, 荒木 駿, 吉田美優, 山本英明, 庭野道夫

    第77回応用物理学会秋季学術講演会　2016/09/13

  100. 人工脂質二分子膜形成のための微細孔を有する半導体シリコンチップの作製プロセスの改善

    但木大介, 平野愛弓, 石橋健一, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫

    第77回応用物理学会秋季学術講演会　2016/09/13

  101. 半導体微細加工を用いた人工細胞膜アレイの開発

    荒木 駿, 平野愛弓, 宮田隆典, 但木大介, 石橋健一, 山本英明, 庭野道夫

    第77回応用物理学会秋季学術講演会　2016/09/13

  102. Fabrication method of silicon chips with micro-apertures for formation of artificial bilayer lipid membranes International-presentation

    D. Tadaki, A. Hirano-Iwata, K. Ishibashi, S. Araki, M. Yoshida, K. Arata, T. Ohori, H. Yamamoto, M. Niwano

    20th International Vacuum Congress (IVC-20)　2016/08/21

  103. Directed polarization and signal propagation in neuronal networks on microcontact printed protein patterns International-presentation

    R. Matsumura, H. Yamamoto, A. Hirano-Iwata, M. Niwano

    20th International Vacuum Congress (IVC-20)　2016/08/21

  104. 神経回路の同期的活動に対するモジュール構造の影響に関する計算論的研究

    守谷 哲, 山本英明, 秋間学尚, 平野愛弓, 庭野道夫, 久保田繁, 佐藤茂雄

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2016/07/04

  105. Spontaneous activity of cultured neuronal networks with defined numbers of neurons: a computational study International-presentation

    H. Yamamoto, Y. Chida, A. Hirano-Iwata, T. Tanii, S. Kubota, M. Niwano

    10th FENS Forum for Neuroscience　2016/07/02

  106. Amplifying neuronal signals in extracellular recordings using electrical resistance of a glial cell sheet International-presentation

    R. Matsumura, H. Yamamoto, A. Hirano-Iwata, M. Niwano

    10th FENS Forum for Neuroscience　2016/07/02

  107. Integration of human ion channels into solvent-free lipid bilayers in microfabricated apertures: accelerated vesicle fusion via centrifugation International-presentation

    A. Hirano-Iwata, M. Yoshida, S. Araki, D. Tadaki, R. Miyata, K. Ishibashi, H. Yamamoto, M. Niwano

    10th FENS Forum for Neuroscience　2016/07/02

  108. Reconstruction of human ion-channels in solvent-free bilayer lipid membranes based on microfabricated silicon chips International-presentation

    M. Yoshida, A. Hirano-Iwata, S. Araki, H. Yamamoto, M. Niwano

    10th FENS Forum for Neuroscience　2016/07/02

  109. 微細加工脂質二分子膜チップへのイオンチャネルの包埋

    平野愛弓, 吉田美優, 荒木 駿, 但木大介, 山本英明, 庭野道夫

    電子情報通信学会 電子デバイス(ED）研究会　2016/04/21

  110. 安定な人工脂質二分子膜形成のための微細孔を有するシリコンチップの作製

    但木大介, 平野愛弓, 石橋健一, 荒木 駿, 吉田美優, 荒田航平, 大堀 健, 山本英明, 庭野道夫

    電子情報通信学会 電子デバイス(ED）研究会　2016/04/21

  111. マイクロパターン表面を用いた培養神経回路の構造-機能制御

    山本英明, 石原広識, 河野翔, 千田雄大, 松村亮佑, 平野愛弓, 久保田繁, 谷井孝至, 庭野道夫

    第63回応用物理学会春季学術講演会　2016/03/19

  112. 細胞シートの電気抵抗を活用した神経細胞外計測の信号増強

    松村亮佑, 山本英明, 平野愛弓, 庭野道夫

    第63回応用物理学会春季学術講演会　2016/03/19

  113. 微細加工イオンチャネルチップにおけるチャネル包埋促進

    平野愛弓, 吉田美優, 荒木 駿, 山本英明, 庭野道夫

    第63回応用物理学会春季学術講演会　2016/03/19

  114. レーザ走査光触媒リソグラフィを活用した液中細胞パターニング

    藤城翔偉, 関根浩平, 河野 翔, 池田 丈, 黒田章夫, 山本英明, 谷井孝至

    第63回応用物理学会春季学術講演会　2016/03/19

  115. Fabrication of planar patch clamp chip by Bosch process and neuron network formation using this chip

    王 志宏, 宇野秀隆, 中尾 聡, 高田紀子, 青山正樹, 鈴井光一, 中原 康, 杉浦広峻, 長谷川貴之, 新井史人, 山本英明, 宇理須恒雄

    第63回応用物理学会春季学術講演会　2016/03/19

  116. 安定化脂質二分子膜イオンチャネルセンサの開発

    吉田美優, 平野愛弓, 山本英明, 庭野道夫

    平成27年度日本表面科学会東北・北海道支部講演会　2016/03/09

  117. ナノテーパー構造に基づく人工細胞膜の構築

    荒木 駿, 平野愛弓, 但木大介, 石橋健一, 山本英明, 庭野道夫

    平成27年度日本表面科学会東北・北海道支部講演会　2016/03/09

  118. Improved process of silicon chips with micro-apertures for formation of mechanically stable bilayer lipid membranes International-presentation

    D. Tadaki, A. Hirano-Iwata, K. Ishibashi, S. Araki, M. Yoshida, K. Arata, T. Ohori, H. Yamamoto, M. Niwano

    7th International Workshop on Nanostructures & Nanoelectronics　2016/03/01

  119. Integration of human ion channels in bilayer lipid membranes formed in microfabricated apertures International-presentation

    A. Hirano-Iwata, M. Yoshida, S. Araki, D. Tadaki, K. Ishibashi, H. Yamamoto, M. Niwano

    7th International Workshop on Nanostructures & Nanoelectronics　2016/03/01

  120. Formation of stable artificial bilayer lipid membranes in nano-tapered microapertures International-presentation

    S. Araki, A. Hirano-Iwata, D. Tadaki, K. Ishibashi, H. Yamamoto, M. Niwano

    7th International Workshop on Nanostructures & Nanoelectronics　2016/03/01

  121. Study on basic characteristics of nano-bubbles generated by porous alumina thin film International-presentation

    N. Yamada, H. Saito, T. Ma, H. Yamamoto, K. Ishibashi, A. Hirano-Iwata, M. Niwano

    7th International Workshop on Nanostructures & Nanoelectronics　2016/03/01

  122. Recording multi- and single-channel currents of human ion-channels in solvent-free bilayer lipid membranes in tapered microapertures International-presentation

    M. Yoshida, S. Araki, A. Hirano-Iwata, H. Yamamoto, M. Niwano

    7th International Workshop on Nanostructures & Nanoelectronics　2016/03/01

  123. Spontaneous activity patterns of small neuronal ensembles International-presentation

    H. Yamamoto, A. Hirano-Iwata, T. Tanii, S. Kubota, M. Niwano

    4th RIEC International Symposium on Brain Functions and Brain Computer　2016/02/23

  124. Surface engineering technologies for constructing and analyzing neuronal circuits International-presentation

    A. Hirano-Iwata, R. Matsumura, H. Yamamoto, M. Niwano

    4th RIEC International Symposium on Brain Functions and Brain Computer　2016/02/23

  125. Equivalent circuit models of covering neurons with a cell sheet for amplifying extracellularly recorded signals International-presentation

    R. Matsumura, H. Yamamoto, M. Niwano, A. Hirano-Iwata

    4th RIEC International Symposium on Brain Functions and Brain Computer　2016/02/23

  126. Live-cell identification of excitatory-inhibitory cell types of cultured cortical neurons focusing on specific axon growth length on micropatterns International-presentation

    S. Kono, T. Kushida, H. Yamamoto, T. Tanii

    4th RIEC International Symposium on Brain Functions and Brain Computer　2016/02/23

  127. Small world-like structure increases synchrony in computational models of cultured neuronal networks International-presentation

    Y. Chida, H. Yamamoto, A. Hirano-Iwata, S. Kubota, M. Niwano

    4th RIEC International Symposium on Brain Functions and Brain Computer　2016/02/23

  128. Effect of network topology on synchronization in modular networks of Kuramoto oscillators International-presentation

    A. F. Shimizu, H. Yamamoto, Y. Chida, A. Hirano-Iwata, S. Kubota, M. Niwano

    4th RIEC International Symposium on Brain Functions and Brain Computer　2016/02/23

  129. Manipulating neuronal cells in situ by TiO2-assisted photocatalytic lithography International-presentation

    H. Yamamoto, A. Hirano-Iwata, T. Tanii, M. Niwano

    9th International Symposium on Nanomedicine (ISNM2015)　2015/12/10

  130. 表面マイクロ改質技術を活用した培養神経細胞/回路の構造制御

    山本英明, 平野愛弓, 谷井孝至, 庭野道夫

    第35回表面科学学術講演会　2015/12/01

  131. 微細加工シリコン基板を用いた人工細胞膜の構築

    荒木駿, 平野愛弓, 山本英明, 庭野道夫

    第35回表面科学学術講演会　2015/12/01

  132. アルミナ多孔質薄膜を用いたナノバブル発生

    山田夏輝, 齋藤栄幸, 馬 騰, 平野愛弓, 山本英明, 石橋健一, 宮澤 誠, 坂本仁志, 庭野道夫

    第35回表面科学学術講演会　2015/12/01

  133. 水クラスターイオンのポリペプチドとの反応の赤外吸収分光その場観察

    吉田真生, 馬 騰, 山本英明, 平野愛弓, 庭野道夫

    第35回表面科学学術講演会　2015/12/01

  134. 位相振動子がつくる複雑ネットワークにおける同期状態の解析

    清水 ファビオ アキラ, 山本英明, 千田雄大, 平野愛弓, 久保田 繁, 庭野道夫

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2015/11/20

  135. 積分発火型ニューロン回路におけるスモールワールド構造と同期の関係

    千田雄大, 山本英明, 平野愛弓, 久保田 繁, 庭野道夫

    電子情報通信学会 ニューロコンピューティング(NC)研究会　2015/11/20

  136. Identification of Normal-Cancer Cells by the Difference in their Adhesion on Organosilane Monolayer Templates: A Feasibility Study International-presentation

    Y. Takeuchi, R. Sakamoto, H. Wakabayashi, H. Yamamoto, Y. Sato, T. Tanii

    28th International Microprocesses and Nanotechnology Conference (MNC 2015)　2015/11/10

  137. Laser-Scanning Photocatalytic Lithography of Organosilane Monolayers for Fabrication of Artificial Neuronal Circuits International-presentation

    K. Sekine, H. Yamamoto, S. Kono, S. Fujishiro, T. Ikeda, A. Kuroda, T. Tanii

    28th International Microprocesses and Nanotechnology Conference (MNC 2015)　2015/11/10

  138. ハイドロゲル膜を用いた神経細胞－グリア細胞の接触共培養系の確立

    松村亮佑, 山本英明, 平野愛弓, 庭野道夫

    第76回応用物理学会秋季学術講演会　2015/09/13

  139. 微細加工シリコン基板に基づくイオンチャネル機能の発現

    吉田美優, 平野愛弓, 山本英明, 庭野道夫

    第76回応用物理学会秋季学術講演会　2015/09/13

  140. マイクロパターン上での培養による興奮性-抑制性神経細胞の非標識判別II

    河野 翔, 櫛田昂歳, 山本英明, 谷井孝至

    第76回応用物理学会秋季学術講演会　2015/09/13

  141. 微細加工シリコン基板を用いたイオンチャネル機能の再構成

    平野愛弓, 山本英明, 木村康男, 庭野道夫

    2015年電子情報通信学会 ソサイエティ大会　2015/09/08

  142. 位相振動子を用いた複数の複雑ネットワーク間の結合による同期状態の制御

    清水 ファビオ アキラ, 山本英明, 千田雄大, 平野愛弓, 久保田 繁, 庭野道夫

    第25回日本神経回路学会全国大会　2015/09/02

  143. 積分発火モデルニューロンにおける同期的活動のネットワーク規模依存性

    千田雄大, 山本英明, 平野愛弓, 谷井孝至, 久保田 繁, 庭野道夫

    第25回日本神経回路学会全国大会　2015/09/02

  144. 培養神経回路の自発発火パターンの素子数依存性に関する理論的考察

    山本英明, 千田雄大, 平野愛弓, 谷井孝至, 久保田 繁, 庭野道夫

    第38回日本神経科学大会　2015/07/28

  145. マウス海馬スライスにおけるキャピラリ型グルタミン酸センサによる前膜からのグルタミン酸放出計測と後シナプス電位の同時測定

    松村亮佑, 平野愛弓, 山本英明, 庭野道夫

    第38回日本神経科学大会　2015/07/28

  146. Quantitative analysis of synapse formation in micropatterned neuronal networks International-presentation

    R. Matsumura, H. Yamamoto, H. Takaoki, S. Katsurabayashi, A. Hirano-Iwata, M. Niwano

    Workshop on Atomically Controlled Processing for Ultra-large Scale Integration　2015/07/09

  147. Reconstitution of ion channel functions in microfabricated silicon chips International-presentation

    A. Hirano-Iwata, H. Yamamoto, Y. Kimura, M. Niwano

    Workshop on Atomically Controlled Processing for Ultra-large Scale Integration　2015/07/09

  148. Live-cell, label free identification of excitatory-inhibitory neurons on micropatterned surfaces International-presentation

    S. Kono, T. Kushida, H. Yamamoto, T. Tanii

    8th International Conference on Molecular Electronics and Bioelectronics (M&BE8)　2015/06/22

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    Student Poster Award受賞

  149. Single-molecule FRET measurements using molecular beacon International-presentation

    T. Kushida, S. Izumi, H. Yamamoto, T. Tanii

    6th International Symposium on Advanced Materials and Development and Integration of Novel Structured Metallic an dInorganic Materials (AMDI-6)　2015/06/09

  150. 微細加工シリコン基板に基づくイオンチャネル機能の再構成

    平野愛弓, 山本英明, 木村康男, 庭野道夫

    電子情報通信学会 電子デバイス(ED）研究会　2015/04/16

  151. 赤外分光法によるナノバブルの表面洗浄効果のその場観察

    齋藤栄幸, 馬 騰, 山本英明, 平野愛弓, 庭野道夫

    電子情報通信学会 電子デバイス(ED）研究会　2015/04/16

  152. マイクロパターン基板上の単一神経細胞間に形成されるシナプスの解析 International-presentation

    高沖英里, 山本英明, 松村亮佑, 桂林秀太郎, 平野愛弓, 庭野道夫

    第62回応用物理学会春季学術講演会　2015/03/11

  153. マイクロパターン神経細胞回路の活動様式に関する理論的考察

    千田雄大, 山本英明, 平野愛弓, 石原広識, 藤森壮也, 谷井孝至, 久保田 繁, 庭野道夫

    第62回応用物理学会春季学術講演会　2015/03/11

  154. モレキュラービーコンを用いた1分子FRET計測系の構築

    和泉聡志, 大久保幸太朗, 櫛田昂歳, 山本英明, 谷井孝至

    第62回応用物理学会春季学術講演会　2015/03/11

  155. 溶液塗布法による有機強誘電体薄膜（PVDF薄膜）の成膜

    中山貴裕, 今井裕司, 馬 騰, 山本英明, 平野愛弓, 庭野道夫

    第62回応用物理学会春季学術講演会　2015/03/11

  156. 規則ナノ細孔を有する多孔質薄膜を用いたナノバブル発生

    齋藤栄幸, 馬 騰, 山本英明, 平野愛弓, 庭野道夫

    第62回応用物理学会春季学術講演会　2015/03/11

  157. 有機シラン単分子膜パターンへの接着差を用いた癌細胞の選別

    竹内祐子, 柿沼瑛介, 坂本留実, 山本英明, 佐藤裕子, 谷井孝至

    第62回応用物理学会春季学術講演会　2015/03/11

  158. 溶液塗布法による有機強誘電体薄膜（PVDF薄膜）の成膜と圧力センサへの応用

    中山貴裕, 今井裕司, 馬 騰, 山本英明, 平野愛弓, 庭野道夫

    平成26年度日本表面科学会東北・北海道支部講演会　2015/03/09

  159. 規則ナノ細孔を有する多孔質薄膜を用いたナノバブル発生

    齋藤栄幸, 馬 騰, 山本英明, 平野愛弓, 庭野道夫

    平成26年度日本表面科学会東北・北海道支部講演会　2015/03/09

  160. Generation of nano-bubbles by a porous film with ordered nanoholes International-presentation

    H. Saito, T. Ma, H. Yamamoto, A. Hirano-Iwata, M. Niwano

    6th International Workshop on Nanostructures & Nanoelectronics　2015/03/02

  161. Chronoamperometric recording of released glutamate after induction of long-term potentiation International-presentation

    R. Matsumura, A. Hirano-Iwata, H. Yamamoto, M. Niwano

    6th International Workshop on Nanostructures & Nanoelectronics　2015/03/02

  162. Recording activities of ion channel proteins in microfablicated silicon chips International-presentation

    A. Hirano-Iwata, Y. Ishinari, H. Yamamoto, Y. Kimura, M. Niwano

    6th International Workshop on Nanostructures & Nanoelectronics　2015/03/02

  163. Single-cell micropatterning techniques for engineering neuronal networks in vitro International-presentation

    H. Yamamoto, A. Hirano-Iwata, M. Niwano, T. Tanii

    3rd RIEC International Symposium on Brain Functions and Brain Computer　2015/02/18

  164. Functional reconstitution of ion channels in artificially formed bilayer lipid membranes International-presentation

    A. Hirano-Iwata, Y. Ishinari, H. Yamamoto, Y. Kimura, M. Niwano

    3rd RIEC International Symposium on Brain Functions and Brain Computer　2015/02/18

  165. Immunocytochemical analysis of synaptogenesis in micropatterned hippocampal neurons International-presentation

    H. Takaoki, H. Yamamoto, R. Matsumura, S. Katsurabayashi, A. Hirano-Iwata, M. Niwano

    3rd RIEC International Symposium on Brain Functions and Brain Computer　2015/02/18

  166. Chronoamperometric recording of increased glutamate release after induction of long-term potentiation in mouse hippocampal slices International-presentation

    R. Matsumura, A. Hirano-Iwata, H. Yamamoto, M. Niwano

    3rd RIEC International Symposium on Brain Functions and Brain Computer　2015/02/18

  167. Computational modeling of the spontaneous activity in micropatterned neuronal networks International-presentation

    Y. Chida, H. Yamamoto, A. Hirano-Iwata, K. Ishihara, S. Fujimori, T. Tanii, S. Kubota, M. Niwano

    3rd RIEC International Symposium on Brain Functions and Brain Computer　2015/02/18

  168. Recording activities of cardiomyocyte channels in microfablicated silicon chips International-presentation

    Y. Ishinari, A. Hirano-Iwata, H. Yamamoto, Y. Kimura, M. Niwano

    3rd RIEC International Symposium on Brain Functions and Brain Computer　2015/02/18

  169. Spontaneous activity patterns of micropatterned neuronal networks in culture International-presentation

    H. Yamamoto, T. Tanii, M. Niwano, A. Hirano-Iwata

    8th International Symposium on Nanomedicine (ISNM2014)　2014/12/04

  170. Adhesibility-Dependent Cell Sorting Using Organosilane Monolayer Template and Green-Tea Epigallocatechin Gallate International-presentation

    R. Sakamoto, E. Kakinuma, Y. Takeuchi, H. Yamamoto, T. Tanii

    5th International Symposium on Advanced Materials Development and Integration of Novel Structural Metallic and Inorganic Materials (AMDI-5)　2014/11/19

  171. In Situ Modification of Cell-Culture Scaffolds by Photocatalytic Decomposition of Organosilane Monolayers on Titanium Dioxide International-presentation

    S. Kono, H. Yamamoto, K. Sekine, T. Tanii

    5th International Symposium on Advanced Materials Development and Integration of Novel Structural Metallic and Inorganic Materials (AMDI-5)　2014/11/19

  172. Optimization of micropattern geometry for long-term culture of isolated neurons and identification of excitatory-inhibitory cell types International-presentation

    H. Yamamoto, S. Kono, T. Kushida, A. Hirano-Iwata, M. Niwano, T. Tanii

    Neuroscience 2014　2014/11/15

  173. 微細加工シリコン基板とイオンチャネルの融合

    石成 裕, 平野愛弓, 山本英明, 木村康男, 庭野道夫

    第34回表面科学学術講演会　2014/11/06

  174. Surface modification in aqueous solution using TiO2 photocatalysis and a linker protein L2 for patterning primary neurons International-presentation

    K. Sekine, H. Yamamoto, S. Kono, T. Ikeda, A. Kuroda, T. Tanii

    7th International Symposium on Surface Science (ISSS-7)　2014/11/02

  175. Interference between Chronoamperometric L-Glutamate Currents and Simultaneously Recorded Field Excitatory Postsynaptic Potentials International-presentation

    R. Matsumura, A. Hirano-Iwata, H. Yamamoto, M. Niwano

    7th International Symposium on Surface Science (ISSS-7)　2014/11/02

  176. Fabrication of micropattered substrate for live-cell, label free identification of excitatory-inhibitory neurons International-presentation

    S. Kono, T. Kushida, H. Yamamoto, T. Tanii

    7th International Symposium on Surface Science (ISSS-7)　2014/11/02

    More details Close

    Travel Award受賞

  177. Microfabricated Si Chips for Reconstitution of Ion Channel Proteins International-presentation

    A. Hirano-Iwata, Y. Ishinari, H. Yamamoto, Y. Kimura, M. Niwano

    7th International Symposium on Surface Science (ISSS-7)　2014/11/02

  178. 培養神経細胞・神経回路操作のための表面マイクロ加工技術

    山本英明, 谷井孝至, 庭野道夫, 平野愛弓

    第52回日本生物物理学会年会　2014/09/25

  179. ナノスリット導波路を用いた細胞内生体分子のリアルタイム蛍光イメージング

    大久保幸太朗, 櫛田昂歳, 和泉聡志, 山本英明, 谷井孝至

    第75回応用物理学会秋季学術講演会　2014/09/17

  180. グルタミン酸電流と興奮性シナプス後電位の同時測定法

    松村亮佑, 平野愛弓, 山本英明, 庭野道夫

    第75回応用物理学会秋季学術講演会　2014/09/17

  181. 有機シラン単分子膜パターン基板を用いたモジュール構造型培養神経回路の構築

    石原広識, 藤森壮也, 山本英明, 谷井孝至

    第75回応用物理学会秋季学術講演会　2014/09/17

  182. マイクロ加工基板を用いた興奮性/抑制性神経細胞判別法の開発

    河野 翔, 櫛田昂歳, 山本英明, 谷井孝至

    第37回日本神経科学大会　2014/09/11

  183. マイクロコンタクトプリンティング法による培養マウス海馬神経細胞の軸索誘導

    高沖英里, 山本英明, 桂林秀太郎, 木村康男, 平野愛弓, 庭野道夫

    第37回日本神経科学大会　2014/09/11

  184. 酸化チタンの光触媒能とリンカータンパク質RPL2を活用した神経細胞パターニング

    関根浩平, 山本英明, 池田 丈, 黒田章夫, 谷井孝至

    第61回応用物理学会春期学術講演会　2014/03/17

  185. マイクロパターン上での培養による興奮性-抑制性神経細胞の非標識判別

    櫛田昂歳, 河野 翔, 山本英明, 谷井孝至

    第61回応用物理学会春期学術講演会　2014/03/17

  186. マイクロパターン上への細胞接着過程のタイムラプス解析 －緑茶カテキンを含む培養液中でのがん細胞と正常細胞の比較－

    坂本留実, 益田顕太朗, 柿沼瑛介, 伊藤耕作, 池滝健太郎, 松崎賢寿, 吉川洋史, 中林誠一郎, 山本英明, 佐藤裕子, 谷井孝至

    第61回応用物理学会春期学術講演会　2014/03/17

  187. 有機単分子膜パターンを用いた細胞接着制御と評価

    谷井孝至, 山本英明

    第61回応用物理学会春期学術講演会　2014/03/17

  188. 表面ナノ改質技術を活用した培養神経回路の構造・機能制御

    山本英明, 谷井孝至

    生物物理学会北海道支部 支部講演会　2014/03/06

  189. Intrinsic activity of defined neuronal networks in culture International-presentation

    H. Yamamoto, T. Tanii

    2nd RIEC International Symposium on Brain Functions and Brain Computer　2014/02/21

  190. Live-cell, label-free identification of excitatory-inhibitory neurons in culture using surface micropatterns International-presentation

    S. Kono, H. Yamamoto, T. Kushida, T. Tanii

    2nd RIEC International Symposium on Brain Functions and Brain Computer　2014/02/21

  191. Application of surface modification techniques in cancer and neuroscience research International-presentation

    T. Tanii, H. Yamamoto

    International Symposium on EcoTopia Science '13　2013/12/13

  192. Directing axon-dendrite polarity of cultured hippocampal neurons with surface micropatterns International-presentation

    H. Yamamoto, S. Kono, T. Demura, M. Morita, G. Banker, T. Tanii, S. Nakamura

    Neuroscience 2013　2013/11/09

  193. Green-tea catechin alters the adhesion state in cancer cells, but not in normal cells International-presentation

    K. Masuda, E. Kakinuma, R. Sakamoto, K. Ito, K. Iketaki, T. Matsuzaki, H. Yoshikawa, S. Nakabayashi, H. Yamamoto, H. T. Chi, Y. Sato, T. Tanii

    5th Int. Conf. on O-CHA (Tea) Culture and Science (ICOS 2013)　2013/11/06

  194. Suppressive effect of epigallocatechin-3-gallate on adhesion of metastatic cancer cells studied by reflection interference contrast microscopy measurement of micropatterned cells International-presentation

    R. Sakamoto, K. Masuda, E. Kakinuma, K. Ito, K. Iketaki, T. Matsuzaki, H. Yoshikawa, S. Nakabayashi, H. Yamamoto, Y. Sato, T. Tanii

    5th Int. Conf. on O-CHA (Tea) Culture and Science (ICOS 2013)　2013/11/06

  195. 有機シラン単分子膜パターン基板を用いた癌細胞の接着能評価

    柿沼 瑛介, 益田 顕太朗, 坂本 留実, 伊藤 耕作, 松崎 賢寿, 吉川 洋史, 中林 誠一郎, 山本 英明, 品田 賢宏, 佐藤 裕子, 谷井 孝至

    第74回応用物理学会秋季学術講演会　2013/09/16

  196. リアルタイム一分子蛍光イメージング法のSN比を向上するゼロモード導波路の設計

    大久保 幸太朗, 日向野 駿, 上野 太郎, 船津 高志, 山本 英明, 谷井 孝至

    第74回応用物理学会秋季学術講演会　2013/09/16

  197. マイクロ加工基板上での単一神経細胞の長期培養

    河野翔, 山本英明, 森田麻裕, 谷井孝至

    第74回応用物理学会秋季学術講演会　2013/09/16

    More details Close

    Poster Award受賞

  198. Fabrication of novel cell chips using in-situ surface modification International-presentation

    T. Tanii, H. Yamamoto

    Collaborative Conference on Materials Research (CCMR) 2013　2013/06/24

  199. 素子数が規定された培養神経細胞ネットワークにおける自発的神経活動の解析

    山本英明, 森田麻裕, 出村崇徳, 河野 翔, 谷井孝至, 中村 俊

    第36回日本神経科学大会　2013/06/20

  200. マイクロパターン基板上の微小神経回路における自発的神経活動

    森田麻裕, 山本英明, 出村崇徳, 河野翔, 中村俊, 谷井孝至

    第60回応用物理学会春期学術講演会　2013/03/27

  201. 酸化チタンの光触媒作用を用いた有機シラン単分子膜の液中分解と細胞培養環境での細胞接着足場改質

    出村崇徳, 山本英明, 森田麻裕, 河野翔, 中村俊, 谷井孝至

    第60回応用物理学会春期学術講演会　2013/03/27

  202. 緑茶カテキンが癌細胞特異的に細胞接着能を抑制する作用機序の調査

    益田顕太朗, 一澤晃太, 柿沼瑛介, 山本英明, Chi Hoang Thanh, 佐藤裕子, 品田賢宏, 谷井孝至

    第60回応用物理学会春期学術講演会　2013/03/27

  203. マイクロ加工基板上にパターニングされた神経細胞における極性形成(Ⅱ)

    河野翔, 山本英明, 出村崇徳, 森田麻裕, 中村俊, 谷井孝至

    第60回応用物理学会春期学術講演会　2013/03/27

  204. 有機シラン単分子膜パターン基板を用いた癌細胞の接着能評価

    柿沼瑛介, 一澤晃太, 益田顕太朗, 山本英明, 品田賢宏, 佐藤裕子, 谷井孝至

    第60回応用物理学会春期学術講演会　2013/03/27

  205. Micropatterned neuronal network in culture as a model system of CNS local circuit International-presentation

    H. Yamamoto, T. Tanii, S. Nakamura

    7th International Symposium on Medical, Bio-, and Nano-Electronics　2013/03/07

  206. In-situ modification of cell-culture scaffolds by TiO2 photocatalysis International-presentation

    H. Yamamoto, T. Demura, M. Morita, S. Kono, S. Nakamura, T. Tanii

    6th International Symposium on Nanomedicine (ISNM2012)　2012/11/29

  207. Surface engineering techniques for designing neuronal networks in vitro International-presentation

    H. Yamamoto, T. Tanii, S. Nakamura

    1st RIEC International Symposium on Brain Functions and Brain Computer　2012/11/15

  208. Photocatalytic Decomposition of Organosilane Monolayer on TiO2 and its Application to In-Situ Modification of a Cell-Culture Surface International-presentation

    T. Demura, H. Yamamoto, M. Morita, S. Kono, S. Nakamura, T. Tanii

    25th International Microprocess and Nanotechnology Conference (MNC 2012)　2012/10/30

  209. 有機シラン単分子膜テンプレートを用いた初代培養神経細胞の極性化誘導

    山本英明

    第1回 ナノメディシン分子科学 若手の会　2012/09/22

  210. 培養海馬神経細胞の軸索決定における突起伸長の役割

    山本英明, 出村崇徳, 森田麻裕, Gary A. Banker, 谷井孝至, 中村 俊

    第35回日本神経科学大会　2012/09/18

  211. 安定な自律的発火リズムを保持するminimal神経回路の解析: ナノ/マイクロ加工技術を用いたアプローチの提案

    山本英明

    第18回情報バイオトロニクス研究会　2012/08/30

  212. 人工神経細胞ネットワークの構築のための要素技術の確立と今後の展開

    山本英明

    第57回ナノ・スピン工学研究会　2012/05/11

  213. 超短パルスレーザー細胞プロセスを利用した神経細胞パターニング

    山本英明

    第1回 超短パルス細胞プロセス研究会　2012/04/13

  214. Fabrication of Artificial Neuronal Circuit by In-Situ Guidance of Individual Neurites Using Photocatalytic Decomposition of Organosilane Monolayer International-presentation

    T. Tanii, T. Demura, M. Morita, H. Yamamoto, S. Nakamura

    3rd International Workshop on Nanostructures & Nanoelectronics　2012/03/21

  215. Surface nano/micro-modification techniques for designing neuronal networks in vitro International-presentation

    H. Yamamoto, T. Demura, M. Morita, T. Tanii, S. Nakamura

    5th International Symposium on Nanomedicine (ISNM2011)　2012/03/15

  216. 有機シラン単分子膜パターン基板を用いた緑茶カテキンを含む培養液中での細胞接着能の評価

    一澤晃太, 益田顕太朗, 山本英明, 佐藤裕子, 谷井孝至

    第59回応用物理学関連連合講演会　2012/03/15

  217. マイクロ加工基板上にパターニングされた神経細胞における極性形成

    山本英明, 出村崇徳, 森田麻裕, Gary Banker, 谷井孝至, 中村 俊

    第59回応用物理学関連連合講演会　2012/03/15

  218. スパッタ法により堆積した酸化チタン膜の光触媒作用を用いた有機シラン単分子膜の液中表面改質と細胞パターニング

    森田麻裕, 出村崇徳, 山本英明, 中村 俊, 谷井孝至

    第59回応用物理学関連連合講演会　2012/03/15

  219. 人工神経細胞回路の構築のための表面ナノ/マイクロ改質技術

    山本英明, 谷井孝至, 中村俊

    第17回情報バイオトロニクス研究会　2012/01/24

  220. Photocatalytic decomposition of organosilane monolayer on TiO2 under aqueous condition and its application to cell patterning International-presentation

    T. Demura, H. Yamamoto, M. Morita, S. Nakamura, T. Tanii

    6th International Symposium on Surface Science　2011/12/11

  221. Effect of Green-Tea Catechins on Normal- and Cancer-Cell Adhesiveness Evaluated Using an Organosilane-Modified Surface Pattern International-presentation

    K. Ichisawa, H. Yamamoto, K. Masuda, T. Demura, M. Morita, Y. Sato, T. Tanii

    6th International Symposium on Surface Science　2011/12/11

  222. In Situ Guidance of Individual Neuronal Processes by Wet Femtosecond-Laser Processing of Self-Assembled Monolayers International-presentation

    H. Yamamoto, K. Okano, T. Demura, Y. Hosokawa, T. Tanii, S. Nakamura

    Materials Research Society Fall Meeting 2011　2011/11/28

  223. Dynamic surface control of culture substrate using a laser-directed microfabrication International-presentation

    K. Okano, A. Matsui, Y. Maezawa, Y. Hosokawa, H. Yamamoto, H. Masuhara, H. Tsubokawa

    Society for Neuroscience Meeting 2011　2011/11/12

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Industrial Property Rights 1

  1. 羽ばたきロボット

    渡邉孝信, 澤根 慧, 富永俊平, 金川 清, 山本英明

    特許第5857658

    Property Type: Patent

Research Projects 26

  1. In vitro reconstitution of bioplausible information processing models and their wetware applications

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Transformative Research Areas (A)

    Institution: Tohoku University

    2024/04/01 - 2029/03/31

  2. Multicellular Neurobiocomputing (Administrative Group)

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Transformative Research Areas (A)

    Institution: Tohoku University

    2024/04/01 - 2029/03/31

  3. Multicellular neurobiocomputing: Understanding and advancing towards biological supremacy

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Transformative Research Areas (A)

    2024/04/01 - 2029/03/31

  4. Mechanisms of neuronal circuit pathogenesis and exploring for therapeutic agents using iPS Cell induced astrocytes from epilepsy patients

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Fukuoka University

    2023/04/01 - 2027/03/31

  5. Artificial reconstitution and reservoir-computing applications of neuronal networks exhibiting high-dimensional dynamics

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2023/04/01 - 2027/03/31

  6. 膜平行電界の実証とそれに基づく膜タンパク質機能計測法への展開

    平野 愛弓, 小宮 麻希, 山本 英明, 但木 大介

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(A)

    Institution: 東北大学

    2023/04/01 - 2026/03/31

  7. Modeling neurodegenerative diseases in artificial neuronal networks

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Institution: Tohoku University

    2022/10/07 - 2026/03/31

  8. ハイブリッド脳の構成と脳型計算機能の検証

    佐藤 茂雄, 山本 英明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2022/06/30 - 2025/03/31

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    本研究では、培養神経細胞を用いて再構成される人工神経回路すなわち培養神経回路と、集積回路上に実現される人工神経回路すなわち半導体神経回路を融合したハイブリッド脳を構成する。研究の目的は、構造可変かつ大規模な生体神経回路を模倣するハイブリッド脳を手段として、生体で実現されている脳型計算機能を検証する。人工的に実現された神経回路において、回路構造と計算機能の関係、学習機能の発現メカニズムなどを実験から明らかにし、半導体神経回路に取り込むべき計算機能の抽出を行う。研究のスケジュールは、最初の１年半でハイブリッド脳の構成を行い、残りの１年半で脳型計算機能の抽出を行う。 本年度は、ハイブリッド脳の構成に向けて、引き続き、半導体神経回路の実装と、培養神経回路の信号計測と刺激印加を同時に行うシステムの構築を行った。半導体神経回路の開発では、サブスレッショルド領域で動作するMOSトランジスタを用いたアナログCMOS回路を用いて、96ニューロンから成る結合が可変なスパイキングニューラルネットワークを構築し、入力信号や結合構造に依存して多様な反応を示すことを確認した。また、FPGAを介して半導体神経回路とPCを接続し、PCから各種制御が可能であることを確認した。培養神経回路の開発では、高密度多点電極アレイを用いたフィードバックシステムを構築し、神経細胞の状態計測と刺激印加が同時に実行できることを確認した。さらに、各種学習則の実装に向けて、必要なソフトウェアの開発を行った。

  9. エッジ応用に向けた超低消費電力スパイキングニューラルネットワークハードウェア

    佐藤 茂雄, 櫻庭 政夫, 山本 英明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  10. Multicellular neurobiocomputing

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Transformative Research Areas (B)

    Category: Grant-in-Aid for Transformative Research Areas (B)

    2021/08/23 - 2024/03/31

  11. Multicellular Neurobiocomputing (Administrative Group)

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Transformative Research Areas (B)

    Institution: Tohoku University

    2021/08/23 - 2024/03/31

  12. In Vitro Reconstitution of Multicellular Computing Systems

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Transformative Research Areas (B)

    Institution: Tohoku University

    2021/08/23 - 2024/03/31

  13. Lateral biasに基づく革新的膜タンパク質機能解析場の構築

    平野 愛弓, 但木 大介, 馬 騰, 山本 英明, 小宮 麻希, 火原 彰秀

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的研究(開拓)

    Category: 挑戦的研究(開拓)

    Institution: 東北大学

    2020/07 - 2024/03

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    Lateral bias印加のための電極内蔵型支持体を作製し，この支持体中での膜形成について検討した．ベース素材としてはシリコン（Si）チップとテフロンフィルムの2種を比較した．その結果，いずれの素材においても脂質二分子膜の形成が可能であったが，Siチップでは脂質分子のみで膜形成が可能であるのに対し，テフロンフィルムでは孔周辺にヘキサデカンの塗布が必要であった．次に，これらの膜系にLateral biasを印加したところ，Siチップ系では交流電圧が，テフロンフィルム系では直流電圧が低ノイズ計測に適していることが分かった．さらに，形成した脂質二分子膜中にフラーレン誘導体（PCBM）や生体イオンチャネルを包埋し，Lateral biasの効果について検討した結果，PCBMドープ膜が示す膜貫通方向の光誘起電流や，電位依存性Naチャネルが示す膜貫通電流が，ともにLateral biasによって増大する現象を見出した（Faraday Discussion, in press, 2022）．しかし，その作用メカニズムは不明であったため，平面脂質二分子膜に対する蛍光観測系を立ち上げ，Lateral biasの作用メカニズムについて蛍光プローブを用いた検討を進めた．また，上述の電極内蔵型支持体では，Lateral biasの印加に伴って電極材料のチタン（Ti）が酸化チタン（TiO2）へと変化するという課題も判明したが，Ti電極を白金（Pt）で被覆することによって酸化を防止できることも分かった．来年度は，電極内蔵型支持体の量産化も進め，メカニズムの検討および応用探索を加速化していく予定である．

  14. Research and Development of Computational Storage Technology to Realize Large Scale Neuron Structure Visualization

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2020/04 - 2024/03

  15. 単一イオンチャネル分子／バイオ二次元物質ハイブリッド膜の機能解析と応用

    平野 愛弓, 但木 大介, 馬 騰, 山本 英明, 小宮 麻希, 戸澤 譲

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(A)

    Category: 基盤研究(A)

    Institution: 東北大学

    2019/04/01 - 2023/03/31

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    脂質二分子膜の膜内に，膜に平行な方向に電圧を印加するための電極内蔵型膜支持体を，シリコン（Si）チップおよびテフロンフィルムの2種類の素材をベース材料として作製した．これらの支持体中での脂質二分子膜形成が，電極の存在によって影響を受けないことを確認した後，膜平行電圧印加に伴う膜電気特性の変化について，ノイズ電流レベル，膜抵抗，電気容量の観点から評価した．さらに，形成した脂質二分子膜中にナノ材料や生体イオンチャネルを包埋し，これらのハイブリッド膜に対する膜平行電圧の効果について検討した．その結果，フラーレン誘導体のPCBMドープ膜が示す膜貫通方向の光応答電流や，電位依存性Naチャネルが示す膜貫通電流が，ともに膜平行電圧の印加によって変調・増強されることを見出した（Faraday Discussion, in press,2022）．この膜平行電圧による膜貫通電流の増強作用や，PCBMドープ膜の光応答電流については，どちらも興味深い現象ではあるが発生メカニズムが不明であったため，2021年度は，脂質二分子膜系に対する蛍光測定系を立ち上げ，これらの現象の作用メカニズムについて蛍光プローブを用いて検討した．また，上述の膜支持体では，チタン（Ti）を電極材料として用いていたが，膜平行電圧の印加に伴って，コンタクト部位が酸化チタン（TiO2）へと変化しやすいことも分かってきた．そこで，Ti電極上への白金（Pt）コーティングを検討した結果，電極の酸化防止に効果的であった．一方，TiO2はそれ自体が魅力的なチューブ状ナノ構造体であり，このナノチューブを利用したガスセンサーの構築（Langmuir, 2021）やナノバブル生成への応用についても検討した（Sensors and Actuators: B, Chemical, in press）．

  16. バイオニック情報処理システムの人工再構成

    山本英明

    Offer Organization: 国立研究開発法人科学技術振興機構（JST）

    System: 戦略的創造研究推進事業

    Category: さきがけ

    Institution: 東北大学

    2018/10 - 2022/03

  17. Time-series information processing based on complex dynamics in artificial neuronal networks and its computational modelling

    Yamamoto Hideaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2018/04 - 2022/03

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    We developed a novel perturbation-analysis setup for artificial neuronal networks to constructively investigate the structure-function relationships in neuronal networks, focusing especially on the modular architecture in the nervous system. Using this setup, we unveiled the functional significance of the modular structure through the analysis of their response to asynchronous input and time-series signals.

  18. Expression of an artificial action potential and its application to screening devices for drug side effects

    Hirano-Iwata Ayumi, NIWANO MICHIO

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    In recent years, the risk evaluation of potential drug side effects to the heart is indispensable in drug discovery, and it is highly desirable to construct an artificial system for this evaluation. In this study, we tried to develop a screening platform for potential drug side effects based on artificially formed bilayer lipid membranes (BLMs) that can express action potentials. However, we faced an issue of a low efficiency in incorporation of ion-channels that are necessary for generation of action potentials. To address this issue, we investigated the process of ion channel incorporation and found that application of appropriate centrifugal forces during the incorporation process lead to a dramatic enhancement of the incorporation probability from ca. 6% to ca. 70%. We also investigated development of high-throughput assay systems for the ion channels and succeeded in simultaneous recordings of hERG channel activities in BLM systems.

  19. Functional analysis of micropatterned neuronal networks using nano-structured electrodes

    Yamamoto Hideaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    Brain is a complex network of nerve cells. Neuronal culture has played important roles in the study of the nerve cells, but the innate difference in network architecture has hindered the wide-spread use of the culture system in the study of network functions. In the current project, we developed a series of surface engineering technologies to direct the spontaneous network formation by cultured neurons. This was realized at both the single-cellular and multi-cellular levels. We also developed a technology for amplifying weak signals of multi-electrode array recordings, that takes advantage of a cell sheet grown on a free-standing hydrogel membrane.

  20. Development of novel techniques for single-molecule fluorescence imaging of various biomolecules

    TANII Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Waseda University

    2014/04/01 - 2017/03/31

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    In order to enhance the ability of single-molecule fluorescence imaging (SMFI) that enables to detect binding and dissociation events of bio-molecules in real time using zero-mode waveguides (ZMWs), we propose a novel technique to combine fluorescence resonance energy transfer (FRET) and SMFI with ZMWs, and proved experimentally that the proposed method is feasible. We also tried to extend the ability of this technique by demonstrating SMFI of individual DNA aptamers for sensing application, SMFI of DNA strand scission by endonuclease, and in-situ cell patterning.

  21. Single electron tunneling in lipid bilayers as a novel sensing principle

    Hirano-Iwata Ayumi, NIWANO MICHIO, KIMURA YASUO, YAMAMOTO HIDEAKI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04/01 - 2016/03/31

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    This study aims at realization of single-electron tunneling in lipid bilayers as a new sensing principle. We first examined incorporation of nanoparticles into lipid bilayers and found that only very small nanoparticles could be incorporated into self-standing bilayer lipid membranes. We next examined simulation on single-electron effects of aligned nanoparticles and revealed that observable single-electron effects would be possible even at room temperature. In addition, we have established fabrication procedures for silicon chips with wired metal electrodes. We would like to combine these techniques to realize single-electron tunneling in lipid bilayers and apply to highly sensitive biosensors to demonstrate a novel sensing principle.

  22. Improvement of in situ surface modification techniques for manipulating neuronal networks

    YAMAMOTO Hideaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up

    Category: Grant-in-Aid for Research Activity Start-up

    2013/08/30 - 2015/03/31

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    Titanium dioxide (TiO2) photocatalysis can be applied to pattern proteins and cells under aqueous solution. Here, we extended the application of this technique to pattern primary neurons. We employed ribosomal protein L2 (RPL2) to stably bind laminin to TiO2. We found that a protein complex consisting of laminin/anti-laminin antibody/protein A-RPL2 is spontaneously formed by simply mixing the precursor proteins in solution phase, and that the surface coated with the protein complex supports stable growth of rat hippocampal neurons. Finally, we showed that the cells can be selectively grown on the protein complex patterned with the TiO2-assisted method. The protocol established here is a unique combination of a top-down micropatterning of the surface using TiO2 photocatalysis and a bottom-up self-assembly of biomolecules, which can be further applied to pattern a wide range of proteins and cells. (Sekine, Yamamoto et al., e-J Surf Sci Nanotechnol 2015)

  23. Fabrication and evaluation of artificial neuronal networks on semiconductor surfaces

    NIWANO Michio, HIRANO Ayumi, KIMURA Yasuo, YAMAMOTO Hideaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    Human brain is a complex, hierarchical system comprised of 10 billion neurons. By engineering a simple neuronal network in vitro that mimics the in vivo architecture and functions, a novel system for studying of the dynamical properties of the neuronal networks in the brain will become available. In the current project, we developed a series of surface micromodification techniques for fabricating "template" surfaces for neuronal cell patterning. We then evaluated the spontaneous activity of the micropatterned neuronal networks via fluorescent calcium imaging. A simulation model of neuronal networks based on a biophysical model was also developed to theoretically investigate activity modulation in micropatterned networks.

  24. Biosensing for nanospace synapses and its application to measurements of brain functions

    HIRANO-IWATA Ayumi, NIWANO Michio, KIMURA Yasuo, AONUMA Yuki, YAMAMOTO Hideaki, SUGAWARA Masao

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    We have developed biosensors for bio-nanospace “synapses” and investigated their application to measurements in brain. We combined amperometric glutamate microsensor and recording of field excitatory postsynaptic potentials (fEPSPs) as a new method for analyzing relation between presynaptic and postsynaptic activities. Although it was found that electric stimuli and evoked fEPSPs induced a capacitive transient current at the microsensor, we proposed a method to extract a faradaic signal from the observed currents. Then we applied this method to long-term potentiation (LTP) in the hippocampus. It was found that the level of the extracellular L-glutamate during tetanus stimulation was significantly related to LTP induction. Thus, our simultaneous recording method for pre- and post-synaptic activities can be a useful tool for neurosciences. We also investigated efficient construction of lipid bilayer-based sensor utilizing ion channel as a sensory element for neurotransmitters.

  25. 表面ナノ改質技術を基盤とした人工神経細胞回路チップの開発とその創薬応用

    山本 英明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東京農工大学

    2010 - 2012

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    中枢神経系の複雑な神経回路網のモデルとなる神経細胞回路を培養系で自在に構築し、そのダイナミクスを評価するための方法論を確立するために、以下の研究課題に取り組んだ。(1)マイクロパターン基板上の微小神経回路における自発的神経活動の解析:マイクロパターン基板上の微小培養神経回路の自発的な活動をカルシウムイメージングによって計測することに成功した。寸法の異なるパターン上の神経回路の活動を比較したところ、神経回路の大きさが増加するとともに自発的バースト活動の頻度が増加することが分かった。また、たかだか十数個の細胞からなる神経回路でも自発活動が発生することを確認することができた。これは、従来考えられていたよりもはるかに少ない数の素子数からなる神経回路であっても、cell assemblyとしての機能を持ちうることを示唆している。(2)酸化チタンの光触媒作用を用いた有機シラン単分子膜の液中分解法の開発:細胞培養環境下で基板表面の細胞接着性を改変できる局所表面改質法は、異種細胞間の相互作用解析や局所神経回路の構築のための細胞操作の自由度を向上させる。本年度は、酸化チタンの光触媒作用を用いて培養液中で有機単分子膜表面(オクタデシルシラン単分子膜)を分解除去できること、それにより基板表面の細胞親和性を局所的に改質できることを、がん細胞株を用いて実証した。改質には、蛍光顕微鏡水銀ランプのUV光、もしくは375nm UVレーザーを用いた。本手法の神経細胞系への適応も進めている。

  26. 有機単分子膜とナノ加工技術を用いた神経細胞軸索の高精度誘導技術の開発

    山本 英明

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 早稲田大学

    2007 - 2009

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    【目的】細胞親和性が局所的に改質された基板上で神経細胞を培養すると、細胞は親和性の高い領域に選択的に接着し、神経突起を伸長させる。本研究ではこの特性を応用し、単一細胞レベルで制御された入出力接続を有する小さな神経回路を、数個の神経細胞を構成要素として再構築するための、ナノ改質基板の開発を行った。 【ナノ改質基板上でのPC12細胞の培養】電子線リソグラフィを用いて、アミノ基末端単分子膜(接着促進領域)およびメチル基末端単分子膜(接着阻害領域)をガラス基板上にパターニングした。アミノ基末端単分子膜は、APTES(aminopropyltriethoxysilane)を前駆体分子として成膜した。細胞培養実験には、神経細胞のモデル細胞として、PC12細胞を用いた。細胞播種条件を整えたのちにナノ改質基板上でPC12細胞を培養したところ、正方形のアミノ基末端単分子膜パターン上に単一のPC12細胞が接着し、またそこから延びる直線パターン上に神経突起を伸長させている様子が確認できた。 【ナノ改質基板上での初代培養神経細胞の培養】続いて、初代培養神経細胞を配列固定するための実験に着手した。神経細胞は胎生9-10日のニワトリ胚の前脳から採取した。APTES上に神経細胞を培養したところ、神経細胞はPC12細胞に比べて接着能力が低く、その上に神経細胞は接着しないことが分かった。そこで、APTESに比べて1分子あたりのアミノ基数が多いDETA(diethylenetriamine)上への神経細胞の接着性を評価し、神経細胞はDETA単分子膜上に非常に強く接着するとこ、そしてDETA単分子膜上で培養を一週間以上に渡って安定に継続できることを確認した。また、DETA/ODSナノ改質基板上に神経細胞を配列固定するための培養条件もほぼ確立した。

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  1. 加藤バイオサイエンス振興財団・国際交流助成：Spontaneous activity of cultured neuronal networks with defined numbers of neurons: a computational study

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    生物の脳を構成する神経細胞の活動は時間的にも空間的にも非ランダムである．特に，複数の細胞が時間的に近いタイミングで発火する現象は同期発火と呼ばれ，信号伝達効率の調整や距離の離れた脳領域間での情報伝達において重要な役割を担っている．しかし，非線形素子である神経細胞の活動がどのように自律的に同期し，またそれを安定に維持するのかは明らかにされていない．そこで本研究では，表面微細加工技術を用いて回路の規模を規定した培養神経回路を用意し，回路を構成する細胞数が活動の同期性に与える影響を調べた．培養神経回路の活動は蛍光Caイメージング法を用いて計測し，さらに計算機シミュレーションを用いて，実験の結果を説明する数理モデルを構築した．

  2. 旭硝子財団・研究奨励：表面工学と脳神経科学の融合によるネットワーク構造を規定した神経細胞回路の作製とその構造-機能相関の解明

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    脳機能の神経基盤の解明は、21世紀の生命科学における最も大きな課題の１つであり、同時に、神経変性疾患や精神疾患に悩む現代社会が抱える喫緊の課題である。 本研究では、ナノ/マイクロスケールでのガラス基板表面の機能化技術を駆使して、複雑な中枢神経系のモデルとなる神経回路を細胞培養系で構築する。この技術を基盤として、生体神経回路様の接続様式を持つ培養神経回路を作製し、神経回路の動作原理の解明において最も重要な、回路構造と機能との相関関係を明らかにする。