Details of the Researcher

PHOTO

Takuma Toyonaga
Section
Institute of Multidisciplinary Research for Advanced Materials
Job title
Assistant Professor

Research History 3

  • 2024/04 - Present
    東北大学 多元物質科学研究所　助教

  • 2022/04 - 2024/03
    大阪公立大学 大学院理学研究科 生物学専攻　助教

  • 2021/10 - 2024/03
    大阪市立大学 大学院理学研究科 生物地球系専攻　助教

Education 2

  • 大阪市立大学　大学院理学研究科　生物地球系専攻

    2015/04 - 2020/03

  • 大阪市立大学　理学部　生物学科

    2011/04 - 2015/03

Research Interests 3

  • 回転分子モーター

  • マイコプラズマ滑走運動

  • クライオ電子顕微鏡

Research Areas 2

  • Life sciences / Bacteriology /

  • Life sciences / Biophysics /

Awards 8

  1. Louis Dienes Award

    2021/11　23th Biennial Congress of the International Organization for Mycoplasmology

  2. Poster Prize

    2019/03　10th OCARINA International Symposium

  3. 優秀発表賞

    2018/03　第91回日本細菌学会総会

  4. Poster Prize

    2017/09　International Symposium on ‟Harmonized supramolecular motility machinery and its diversity"

  5. 学生発表賞

    2017/09　第55回日本生物物理学会年会

  6. Poster Prize

    2017/03　8th OCARINA International Symposium

  7. 学生発表賞

    2016/11　第54回日本生物物理学会年会

  8. 優秀発表賞

    2016/06　第43回日本マイコプラズマ学会学術集会

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Papers 8

  1. Dimeric assembly of F1-like ATPase for the gliding motility of Mycoplasma Peer-reviewed

    Takuma Toyonaga, Takayuki Kato, Akihiro Kawamoto, Tomoko miyata, Keisuke Kawakami, Junso Fujita, Tasuku Hamaguchi, Keiichi Namba, Makoto Miyata

    Science Advances　11　(9)　2025/02/26

    DOI： 10.1126/sciadv.adr9319 　

  2. Assembly Formation of P65 Protein, Featured by an Intrinsically Disordered Region Involved in Gliding Machinery of Mycoplasma pneumoniae Peer-reviewed

    Masaru Yabe, Takuma Toyonaga, Miki Kinoshita, Yukio Furukawa, Tasuku Hamaguchi, Yuhei O. Tahara, Munehito Arai, Katsumi Imada, Makoto Miyata

    Biomolecules　15　(3)　429-429　2025/02/17

    Publisher: MDPI AG

    DOI： 10.3390/biom15030429 　

    eISSN： 2218-273X

    More details Close

    Mycoplasma pneumoniae is a human pathogen that glides on host cell surfaces by a repeated catch and release mechanism using sialylated oligosaccharides. At a pole, this organism forms a protrusion called an attachment organelle composed of surface structures, including an adhesin complex and an internal core structure. To clarify the structure and function of the attachment organelle, we focused on a core component, P65, which is essential for stabilization of the adjacent surface and core proteins P30 and HMW2, respectively. Analysis of its amino acid sequence (405 residues) suggested that P65 contains an intrinsically disordered region (residues 1–217) and coiled-coil regions (residues 226–247, 255–283, and 286–320). Four protein fragments and the full-length P65 were analyzed by size exclusion chromatography, analytical centrifugation, circular dichroism spectroscopy, small-angle X-ray scattering, limited proteolysis, and negative staining electron microscopy. The results showed that P65 formed a multimer composed of a central globule with 30 and 23 nm axes and four to six projections 14 nm in length. Our data suggest that the C-terminal region of P65 is responsible for multimerization, while the intrinsically disordered N-terminal region forms a filament. These assignments and roles of P65 in the attachment organelle are discussed.

  3. Internal structure of Mycoplasma mobile gliding machinery analyzed by negative staining electron tomography Peer-reviewed

    Minoru Fukushima, Takuma Toyonaga, Yuhei O. Tahara, Daisuke Nakane, Makoto Miyata

    Biophysics and Physicobiology　21　(2)　2024/02/09

    DOI： 10.1101/2024.02.08.578511 　

  4. Purification and Structural Analysis of the Gliding Motility Machinery in Mycoplasma mobile Peer-reviewed

    Takuma Toyonaga, Makoto Miyata

    Methods in Molecular Biology　2023

    DOI： 10.1007/978-1-0716-3060-0_25 　

    ISSN： 1064-3745 1940-6029

  5. Chained Structure of Dimeric F1-like ATPase in Mycoplasma mobile Gliding Machinery Peer-reviewed

    Takuma Toyonaga, Takayuki Kato, Akihiro Kawamoto, Noriyuki Kodera, Tasuku Hamaguchi, Yuhei O. Tahara, Toshio Ando, Keiichi Namba, Makoto Miyata

    mBio　12　(4)　2021/08/31

    Publisher: American Society for Microbiology

    DOI： 10.1128/mBio.01414-21 　

  6. Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy Peer-reviewed

    Kohei Kobayashi, Noriyuki Kodera, Taishi Kasai, Yuhei O Tahara, Takuma Toyonaga, Masaki Mizutani, Ikuko Fujiwara, Toshio Ando, Makoto Miyata

    mBio　2021/06/29

    DOI： 10.1128/mbio.00040-21 　

    ISSN： 2150-7511

    More details Close

    <jats:p> The <jats:italic>Mycoplasma</jats:italic> genus contains bacteria generally parasitic to animals and plants. Some <jats:italic>Mycoplasma</jats:italic> species form a protrusion at a pole, bind to solid surfaces, and glide by a special mechanism linked to their infection and survival. </jats:p>

  7. Refined Mechanism of Mycoplasma mobile Gliding Based on Structure, ATPase Activity, and Sialic Acid Binding of Machinery Peer-reviewed

    Miyuki S. Nishikawa, Daisuke Nakane, Takuma Toyonaga, Akihiro Kawamoto, Takayuki Kato, Keiichi Namba, Makoto MIYATA

    mBio　2019/12/24

    DOI： 10.1128/mbio.02846-19 　

    ISSN： 2161-2129 2150-7511

    More details Close

    <jats:p> The genus <jats:italic>Mycoplasma</jats:italic> is made up of the smallest parasitic and sometimes commensal bacteria; <jats:named-content content-type="genus-species">Mycoplasma pneumoniae</jats:named-content> , which causes human “walking pneumonia,” is representative. More than ten <jats:italic>Mycoplasma</jats:italic> species glide on host tissues by novel mechanisms, always in the direction of the distal side of the machinery. <jats:named-content content-type="genus-species">Mycoplasma mobile</jats:named-content> , the fastest species in the genus, catches, pulls, and releases sialylated oligosaccharides (SOs), the carbohydrate molecules also targeted by influenza viruses, by means of a specific receptor and using ATP hydrolysis for energy. Here, the architecture of the gliding machinery was visualized three dimensionally by electron cryotomography (ECT), and changes in the structure and binding activity coupled to ATP hydrolysis were discovered. Based on the results, a refined mechanism was proposed for this unique motility. </jats:p>

  8. 高速AFMが捕らえた！Mycoplasma mobileの滑走装置 Peer-reviewed

    小林 昂平, 古寺 哲幸, 田原 悠平, 豊永 拓真, 笠井 大司, 安藤 敏夫, 宮田 真人

    顕微鏡　54　(2)　2019

    DOI： 10.11410/kenbikyo.54.2_67 　

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Misc. 4

  1. Structure of Mycoplasma Gliding Motor Evolved from ATP Synthase Invited Peer-reviewed

    Takuma TOYONAGA, Makoto MIYATA

    Seibutsu Butsuri　63　(5)　270-272　2023

    Publisher: Biophysical Society of Japan

    DOI： 10.2142/biophys.63.270 　

    ISSN： 0582-4052

    eISSN： 1347-4219

  2. ATP合成酵素から進化したMycoplasma mobile滑走運動のモーター—Motor evolved from ATP synthase for Mycoplasma mobile gliding—第49回日本マイコプラズマ学会学術集会

    豊永 拓真, 加藤 貴之, 川本 晃大, 宮田 知子, 浜口 祐, 川上 恵典, 難波 啓一, 宮田 真人

    日本マイコプラズマ学会雑誌 = Japanese journal of mycoplasmology　(49)　34-37　2022/05

    Publisher: 長崎 : 日本マイコプラズマ学会事務局

    ISSN： 1340-2382

  3. 生態,生理・構造,遺伝・ゲノミクス・バイオテクノロジー 高速原子間力顕微鏡が捕らえた! マイコプラズマモービレ滑走装置の動き(Movement of Gliding Motors in Mycoplasma mobile Visualized by High-speed Atomic Force Microscopy)

    小林 昂平, 古寺 哲幸, 田原 悠平, 豊永 拓真, 笠井 大司, 安藤 敏夫, 宮田 真人

    日本細菌学雑誌　74　(1)　39-39　2019/03

    Publisher: 日本細菌学会

    ISSN： 0021-4930

    eISSN： 1882-4110

  4. Mycoplasma mobile滑走モーターの三次元像—Three-dimensional image of Mycoplasma mobile gliding motor—第44回日本マイコプラズマ学会学術集会

    豊永 拓真, 加藤 貴之, 川本 晃大, 浜口 祐, 田原 悠平, 難波 啓一, 宮田 真人

    日本マイコプラズマ学会雑誌 = Japanese journal of mycoplasmology　(44)　56-58　2017/05/27

    Publisher: 長崎 : 日本マイコプラズマ学会事務局

    ISSN： 1340-2382

Presentations 14

  1. Cryo-EM structural analysis of the rotary ATPase supercomplex driving Mycoplasma gliding motility Invited

    Takuma Toyonaga, Daisuke Unabara, Tasuku Hamaguchi, Koji Yonekura

    2026/05/25

  2. クライオFIB-SEMおよびクライオミクロトームを用いた納豆菌芽胞の観察

    丹羽 瑠美, 濵口 祐, 豊永 拓真, 海原 大輔, 川上 恵典, 勝部 哲, 米倉 功治

    公益社団法人日本顕微鏡学会 第68回シンポジウム　2025/11/13

  3. 細胞壁欠失細菌マイコプラズマの独自進化したリボソームの構造解析

    室澤 皓暉, 池内 健, 豊永 拓真, 海原 大輔, 濵口 祐, 米倉 功治

    公益社団法人日本顕微鏡学会 第68回シンポジウム　2025/11/13

  4. Integrated cryo-EM structure of G1-ATPase motor for Mycoplasma gliding motility Invited

    Takuma Toyonaga

    NIPS CryoEM workshop 2025, Frontiers of cryo-electron microscopy and tomography　2025/10/29

  5. Cryo-EM visualization of the conformational states of G1-ATPase, a rotary motor driving Mycoplasma mobile gliding

    Takuma Toyonaga, Daisuke Unabara, Tasuku Hamaguchi, Koji Yonekura

    2025/09/26

  6. マイコプラズマ滑走運動を駆動する回転モーターG1-ATPaseの構造解析 Invited

    豊永 拓真

    第14回 分子モーター討論会　2025/09/11

  7. Structural Basis of the Mycoplasma mobile Ribosomes Revealed by Cryo-electron Microscopy

    Murosawa K., Ikeuchi K., Toyonaga T., Unabara D., Hamaguchi T., Yonekura K.

    2025/07/08

  8. 低GC生物マイコプラズマ由来新規Cas9の探索

    上野 莉香子, 池内 健, 豊永 拓真, 濵口 祐, 米倉 功治

    公益社団法人日本顕微鏡学会 第81回学術講演会　2025/06/11

  9. CryoEMによって可視化したマイコプラズマ滑走型Rotary ATPaseの回転

    豊永拓真, 海原大輔, 濵口 祐, 米倉功治

    公益社団法人日本顕微鏡学会 第81回学術講演会　2025/06/09

  10. Cryo-EM Analysis of Chained G1-ATPase, Motor for Mycoplasma Gliding Invited

    Takuma Toyonaga, Daisuke Unabara, Tasuku Hamaguchi, Makoto Miyata, Koji Yonekura

    Sixth Japan-Canada Microscopy Societies Joint Symposium 2025　2025/06/10

  11. マイコプラズマ滑走運動を駆動する新奇回転分子モーターのCryoEM構造

    豊永拓真, 濵口 祐, 宮田真人, 米倉功治

    第24回東北大学多元物質科学研究所研究発表会　2024/12/12

  12. CryoEM structure of dimeric F1-like ATPase in Mycoplasma mobile suggests a rotary catalytic mechanism for the gliding motility

    Takuma TOYONAGA, Takayuki KATO, Akihiro KAWAMOTO, Tomoko MIYATA, Keisuke KAWAKAMI, Junso FUJITA, Tasuku HAMAGUCHI, Keiichi NAMBA, Makoto MIYATA

    21st IUPAB Congress 2024　2024/06/28

  13. Visualization and analysis of MreBs driving Spiroplasma motility in minimal synthetic bacterium

    2024/05/17

  14. Structural analysis of the gliding machinery of Mycoplasma mobile by cryo-electron tomography

    2024/05/17

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Research Projects 1

  1. 細菌滑走運動を駆動する新型Rotary ATPaseの膜を越えた力伝達機構の解明

    豊永 拓真

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 若手研究

    Institution: 東北大学

    2026/04/01 - 2028/03/31