Details of the Researcher

PHOTO

Akiko Inagaki
Section
Graduate School of Medicine
Job title
Assistant Professor
Degree

  • 博士（理学）（石巻専修大学）

  • 修士（理学）（石巻専修大学）

Professional Memberships 3

  • 日本組織移植学会

    2022/04 - Present

  • 日本膵膵島移植学会

    2017/10 -

  • 日本再生医療学会

Research Interests 1

  • 細胞デバイス 膵島移植 肝細胞移植 再生医療 糖尿病治療 細胞治療

Research Areas 1

  • Life sciences / General surgery, pediatric surgery /

Awards 2

  1. 優秀演題賞

    2020/02　第22回日本異種移植研究会　新規免疫隔離膵島デバイスにおける膵島生着効率の検討

  2. 優秀演題賞

    2018/03　第45回日本膵・膵島移植研究会 (JPITA)　免疫抑制剤を不要とする革新的膵島デバイスの確認

Papers 35

  1. A Recombinant Peptide Device Combined with Adipose Tissue-Derived Stem Cells Enhances Subcutaneous Islet Engraftment. International-journal

    Takahiro Mizui, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Satomi Suzuki Uematsu, Shigehito Miyagi, Takashi Kamei, Michiaki Unno, Kimiko Watanabe, Masafumi Goto

    Cells　13　(6)　2024/03/13

    DOI： 10.3390/cells13060499 　

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    Subcutaneous space has been considered an attractive site for islet graft transplantation; however, the oxygen tension and vascularization are insufficient for islet graft survival. We investigated whether subcutaneous pre-implantation of a recombinant peptide (RCP) device with adipose tissue-derived stem cells (ADSCs) enhanced subcutaneous islet engraftment. RCP devices with/without syngeneic ADSCs were pre-implanted into the subcutaneous space of C57BL/6 mice. Syngeneic islets (300 or 120 islet equivalents (IEQs)) were transplanted into the pre-treated space after diabetes induction using streptozotocin. The cure rates of groups in which RCP devices were implanted four weeks before transplantation were significantly better than the intraportal transplantation group when 300 IEQs of islets were transplanted (p < 0.01). The blood glucose changes in the RCP+ADSCs-4w group was significantly ameliorated in comparison to the RCP-4w group when 120 IEQs of islets were transplanted (p < 0.01). Immunohistochemical analyses showed the collagen III expression in the islet capsule of the RCP+ADSCs-4w group was significantly enhanced in comparison to the RCP-4w and RCP+ADSCs-d10 groups (p < 0.01, p < 0.01). In addition, the number of von Willebrand factor-positive vessels within islets in the RCP+ADSCs-4w group was significantly higher than the RCP-4w group. These results suggest that using ADSCs in combination with an RCP device could enhance the restoration of the extracellular matrices, induce more efficient prevascularization within islets, and improve the graft function.

  2. A Gelatin Hydrogel Nonwoven Fabric Combined With Adipose Tissue-Derived Stem Cells Enhances Subcutaneous Islet Engraftment. International-journal

    Ryusuke Saito, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Norifumi Kanai, Hiroaki Mitsugashira, Yukiko Endo Kumata, Takumi Katano, Shoki Suzuki, Kazuaki Tokodai, Takashi Kamei, Michiaki Unno, Kimiko Watanabe, Yasuhiko Tabata, Masafumi Goto

    Cell transplantation　33　9636897241251621-9636897241251621　2024

    DOI： 10.1177/09636897241251621 　

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    Subcutaneous islet transplantation is a promising treatment for severe diabetes; however, poor engraftment hinders its prevalence. We previously revealed that a gelatin hydrogel nonwoven fabric (GHNF) markedly improved subcutaneous islet engraftment. We herein investigated whether the addition of adipose tissue-derived stem cells (ADSCs) to GHNF affected the outcome. A silicone spacer sandwiched between two GHNFs with (AG group) or without (GHNF group) ADSCs, or a silicone spacer alone (Silicone group) was implanted into the subcutaneous space of healthy mice at 6 weeks before transplantation, then diabetes was induced 7 days before transplantation. Syngeneic islets were transplanted into the pretreated space. Intraportal transplantation (IPO group) was also performed to compare the transplant efficiency. Blood glucose, intraperitoneal glucose tolerance, immunohistochemistry, and inflammatory mediators were evaluated. The results in the subcutaneous transplantation were compared using the Silicone group as a control. The results of the IPO group were also compared with those of the AG group. The AG group showed significantly better blood glucose changes than the Silicone and the IPO groups. The cure rate of AG group (72.7%) was the highest among the groups (GHNF; 40.0%, IPO; 40.0%, Silicone; 0%). The number of vWF-positive vessels in the subcutaneous space of the AG group was significantly higher than that in other groups before transplantation (P < 0.01). Lectin angiography also showed that the same results (P < 0.05). According to the results of the ADSCs tracing, ADSCs did not exist at the transplant site (6 weeks after implantation). The positive rates for laminin and collagen III constructed around the transplanted islets did not differ among groups. Inflammatory mediators were higher in the Silicone group, followed by the AG and GHNF groups. Pretreatment using bioabsorbable scaffolds combined with ADSCs enhanced neovascularization in subcutaneous space, and subcutaneous islet transplantation using GHNF with ADSCs was superior to intraportal islet transplantation.

  3. A Gelatin Hydrogel Nonwoven Fabric Enhances Subcutaneous Islet Engraftment in Rats. International-journal

    Ryusuke Saito, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Norifumi Kanai, Hiroaki Mitsugashira, Yukiko Endo Kumata, Takumi Katano, Shoki Suzuki, Kazuaki Tokodai, Takashi Kamei, Michiaki Unno, Kimiko Watanabe, Yasuhiko Tabata, Masafumi Goto

    Cells　13　(1)　2023/12/26

    DOI： 10.3390/cells13010051 　

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    Although subcutaneous islet transplantation has many advantages, the subcutaneous space is poor in vessels and transplant efficiency is still low in animal models, except in mice. Subcutaneous islet transplantation using a two-step approach has been proposed, in which a favorable cavity is first prepared using various materials, followed by islet transplantation into the preformed cavity. We previously reported the efficacy of pretreatment using gelatin hydrogel nonwoven fabric (GHNF), and the length of the pretreatment period influenced the results in a mouse model. We investigated whether the preimplantation of GHNF could improve the subcutaneous islet transplantation outcomes in a rat model. GHNF sheets sandwiching a silicone spacer (GHNF group) and silicone spacers without GHNF sheets (control group) were implanted into the subcutaneous space of recipients three weeks before islet transplantation, and diabetes was induced seven days before islet transplantation. Syngeneic islets were transplanted into the space where the silicone spacer was removed. Blood glucose levels, glucose tolerance, immunohistochemistry, and neovascularization were evaluated. The GHNF group showed significantly better blood glucose changes than the control group (p < 0.01). The cure rate was significantly higher in the GHNF group (p < 0.05). The number of vWF-positive vessels was significantly higher in the GHNF group (p < 0.01), and lectin angiography showed the same tendency (p < 0.05). The expression of laminin and collagen III around the transplanted islets was also higher in the GHNF group (p < 0.01). GHNF pretreatment was effective in a rat model, and the main mechanisms might be neovascularization and compensation of the extracellular matrices.

  4. A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation. International-journal

    Norifumi Kanai, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Hiroaki Mitsugashira, Ryusuke Saito, Shigehito Miyagi, Kimiko Watanabe, Takashi Kamei, Michiaki Unno, Yasuhiko Tabata, Masafumi Goto

    Scientific reports　13　(1)　11968-11968　2023/07/24

    DOI： 10.1038/s41598-023-39212-4 　

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    Subcutaneous islet transplantation is a promising treatment for severe diabetes; however, poor engraftment hinders its prevalence. We previously reported that a recombinant peptide (RCP) enhances subcutaneous islet engraftment. However, it is impractical for clinical use because RCP must be removed when transplanting islets. We herein investigated whether a novel bioabsorbable gelatin hydrogel nonwoven fabric (GHNF) could improve subcutaneous islet engraftment. A silicon spacer with or without GHNF was implanted into the subcutaneous space of diabetic mice. Syngeneic islets were transplanted into the pretreated space or intraportally (Ipo group). Blood glucose, intraperitoneal glucose tolerance, immunohistochemistry, CT angiography and gene expression were evaluated. The cure rate and glucose tolerance of the GHNF group were significantly better than in the control and Ipo groups (p < 0.01, p < 0.05, respectively). In the GHNF group, a limited increase of vWF-positive vessels was detected in the islet capsule, whereas laminin (p < 0.05), collagen III and IV were considerably enhanced. TaqMan arrays revealed a significant upregulation of 19 target genes (including insulin-like growth factor-2) in the pretreated space. GHNF markedly improved the subcutaneous islet transplantation outcomes, likely due to ECM compensation and protection of islet function by various growth factors, rather than enhanced neovascularization.

  5. Pancreatic Islet Transplantation into the Submandibular Gland: Our Experimental Experience and a Review of the Relevant Literature International-journal

    Ibrahim Fathi, Akiko Inagaki, Takehiro Imura, Tarek Koraitim, Masafumi Goto

    Journal of Clinical Medicine　12　(11)　3735-3735　2023/05/29

    Publisher: MDPI AG

    DOI： 10.3390/jcm12113735 　

    eISSN： 2077-0383

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    Pancreatic islet transplantation is a promising therapy for type 1 diabetes. Islet transplantation is clinically performed through intra-portal infusion, which is associated with several drawbacks, including poor engraftment. The histological resemblance between the submandibular gland and the pancreas renders it an attractive alternative site for islet transplantation. In this study, we refined the technique of islet transplantation into the submandibular gland to achieve good morphological features. Then, we transplanted 2600 islet equivalents into the submandibular glands of diabetic Lewis rats. Intra-portal islet transplantation was performed in diabetic rats as a control. Blood glucose levels were followed for 31 days, and an intravenous glucose tolerance test was performed. Immunohistochemistry was used to demonstrate the morphology of transplanted islets. Follow-up after transplantation showed that diabetes was cured in 2/12 rats in the submandibular group in comparison to 4/6 in the control group. The intravenous glucose tolerance test results of the submandibular and intra-portal groups were comparable. Immunohistochemistry showed large islet masses in the submandibular gland in all examined specimens with positive insulin staining. Our results show that submandibular gland tissue can support the islet function and engraftment but with considerable variability. Good morphological features were achieved using our refined technique. However, islet transplantation into rat submandibular glands did not demonstrate a clear advantage over conventional intra-portal transplantation.

  6. Ideal Duration of Pretreatment Using a Gelatin Hydrogel Nonwoven Fabric Prior to Subcutaneous Islet Transplantation. International-journal

    Ryusuke Saito, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Norifumi Kanai, Hiroaki Mitsugashira, Yukiko Endo, Takumi Katano, Shoki Suzuki, Kazuaki Tokodai, Takashi Kamei, Michiaki Unno, Kimiko Watanabe, Yasuhiko Tabata, Masafumi Goto

    Cell transplantation　32　9636897231186063-9636897231186063　2023

    DOI： 10.1177/09636897231186063 　

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    Subcutaneous islet transplantation is a promising treatment for severe diabetes; however, poor engraftment hinders its prevalence. We previously revealed that a gelatin hydrogel nonwoven fabric (GHNF) markedly improved subcutaneous islet engraftment in comparison with intraportal islet transplantation. We herein investigated whether the duration of pretreatment using GHNF affected the outcome of subcutaneous islet transplantation. A silicone spacer with GHNF was implanted into the subcutaneous space of healthy mice at 2, 4, 6, or 8 weeks before transplantation, and then diabetes was induced 7 days before transplantation. Syngeneic islets were transplanted into the pretreated space. Blood glucose, intraperitoneal glucose tolerance, immunohistochemistry, inflammatory mediators, and gene expression were evaluated. The 6-week group showed significantly better blood glucose changes than the other groups (P < 0.05). The cure rate of the 6-week group (60.0%) was the highest among the groups (2-week = 0%, 4-week = 50.0%, 8-week = 15.4%). The number of von Willebrand factor (vWF)-positive vessels in the 6-week group was significantly higher than in the other groups at pre-islet and post-islet transplantation (P < 0.01 [vs 2-and 4-week groups] and P < 0.05 [vs all other groups], respectively). Notably, this beneficial effect was also observed when GHNF was implanted into diabetic mice injected with streptozotocin 7 days before GHNF implantation. The positive rates for laminin, collagen III, and collagen IV increased as the duration of pretreatment became longer and were significantly higher in the 8-week group (P < 0.01). Inflammatory mediators, including interleukin (IL)-1b, granulocyte colony-stimulating factor (G-CSF), and interferon (IFN)-γ, were gradually downregulated according to the duration of GHNF pretreatment and re-elevated in the 8-week group. Taken together, the duration of GHNF pretreatment apparently had an impact on the outcomes of subcutaneous islet transplantation, and 6 weeks appeared to be the ideal duration. Islet graft revascularization, extracellular matrix compensation of the islet capsule, and the inflammatory status at the subcutaneous space would be crucial factors for successful subcutaneous islet transplantation.

  7. Development of a novel method for measuring tissue oxygen pressure to improve the hypoxic condition in subcutaneous islet transplantation International-journal

    Hiroaki Mitsugashira, Takehiro Imura, Akiko Inagaki, Yukiko Endo, Takumi Katano, Ryusuke Saito, Shigehito Miyagi, Kimiko Watanabe, Takashi Kamei, Michiaki Unno, Masafumi Goto

    Scientific Reports　12　(1)　14731-14731　2022/08/30

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-022-19189-2 　

    eISSN： 2045-2322

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    Abstract Subcutaneous tissue is a promising site for islet transplantation, but poor engraftment, due to hypoxia and low vascularity, hinders its prevalence. However, oxygen partial pressure (pO2) of the subcutaneous space (SC) and other sites were reported to be equivalent in several previous reports. This contradiction may be based on accidental puncture to the indwelling micro-vessels in target tissues. We therefore developed a novel optical sensor system, instead of a conventional Clark-type needle probe, for measuring tissue pO2 and found that pO2 of the SC was extremely low in comparison to other sites. To verify the utility of this method, we transplanted syngeneic rat islets subcutaneously into diabetic recipients under several oxygenation conditions using an oxygen delivery device, then performed pO2 measurement, glucose tolerance, and immunohistochemistry. The optical sensor system was validated by correlating the pO2 values with the transplanted islet function. Interestingly, this novel technique revealed that islet viability estimated by ATP/DNA assay reduced to less than 75% by hypoxic condition at the SC, indicating that islet engraftment may substantially improve if the pO2 levels reach those of the renal subcapsular space. Further refinements for a hypoxic condition using the present technique may contribute to improving the efficiency of subcutaneous islet transplantation.

  8. Use of Perfluorohexyloctane for Preservation of Rat Liver After Circulatory Death and a Prolonged Cold Preservation Model for Hepatocyte Transplantation

    Muneyuki Matsumura, Takehiro Imura, Akiko Inagaki, Hiroyuki Ogasawara, Shigehito Miyagi, Kazuo Ohashi, Michiaki Unno, Takashi Kamei, Masafumi Goto

    Transplant International　35　2022/07/05

    Publisher: Frontiers Media SA

    DOI： 10.3389/ti.2022.10659 　

    eISSN： 1432-2277

  9. KRP-203 Is a Desirable Immunomodulator for Islet Allotransplantation. International-journal

    Ibrahim Fathi, Ryuichi Nishimura, Takehiro Imura, Akiko Inagaki, Norifumi Kanai, Akira Ushiyama, Masafumi Kikuchi, Masamitsu Maekawa, Hiroaki Yamaguchi, Masafumi Goto

    Transplantation　106　(5)　963-972　2022/05/01

    DOI： 10.1097/TP.0000000000003870 　

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    BACKGROUND: The current standard immunosuppressive regimens, calcineurin inhibitors, have diabetogenic and anti-vascularization effects on islet grafts. KRP-203, a sphingosine-1-phosphate functional antagonist, exerts its immunomodulatory function through lymphocyte sequestration. However, the effect of this antagonist on islets is unclear. We examined the effect of KRP-203 on the islet function and vascularization and sought a calcineurin-free regimen for islet allotransplantation. METHODS: KRP-203 was administered for 14 d to mice, then diabetogenic effect was evaluated by blood glucose levels and a glucose tolerance test. Static glucose stimulation, the breathing index, and insulin/DNA were examined using isolated islets. Islet neovascularization was evaluated using a multiphoton laser scanning microscope. After islet allotransplantation with either KRP-203 alone, sirolimus alone, or both in combination, the graft survival was evaluated by blood glucose levels and immunohistochemical analyses. A mixed lymphocyte reaction was also performed to investigate the immunologic characteristics of KRP-203 and sirolimus. RESULTS: No significant differences in the blood glucose levels or glucose tolerance were observed between the control and KRP-203 groups. Functional assays after islet isolation were also comparable. The multiphoton laser scanning microscope showed no inhibitory effect of KRP-203 on islet neovascularization. Although allogeneic rejection was effectively inhibited by KRP-203 monotherapy (44%), combination therapy prevented rejection in most transplanted mice (83%). CONCLUSIONS: KRP-203 is a desirable immunomodulator for islet transplantation because of the preservation of the endocrine function and lack of interference with islet neovascularization. The combination of KRP-203 with low-dose sirolimus may be promising as a calcineurin-free regimen for islet allotransplantation.

  10. Cotransplantation With Adipose Tissue-Derived Stem Cells Improves Engraftment of Transplanted Hepatocytes. International-journal

    Hiroki Yamana, Akiko Inagaki, Takehiro Imura, Yasuhiro Nakamura, Hiroyasu Nishimaki, Takumi Katano, Kazuo Ohashi, Shigehito Miyagi, Takashi Kamei, Michiaki Unno, Masafumi Goto

    Transplantation　106　(10)　1963-1973　2022/04/11

    DOI： 10.1097/TP.0000000000004130 　

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    BACKGROUND: Hepatocyte transplantation is expected to be an alternative therapy to liver transplantation; however, poor engraftment is a severe obstacle to be overcome. The adipose tissue-derived stem cells (ADSCs) are known to improve engraftment of transplanted pancreatic islets, which have many similarities to the hepatocytes. Therefore, we examined the effects and underlying mechanisms of ADSC cotransplantation on hepatocyte engraftment. METHODS: Hepatocytes and ADSCs were cotransplanted into the renal subcapsular space and livers of syngeneic analbuminemic rats, and the serum albumin level was quantified to evaluate engraftment. Immunohistochemical staining and fluorescent staining to trace transplanted cells in the liver were also performed. To investigate the mechanisms, cocultured supernatants were analyzed by a multiplex assay and inhibition test using neutralizing antibodies for target factors. RESULTS: Hepatocyte engraftment at both transplant sites was significantly improved by ADSC cotransplantation (P < 0.001, P < 0.001). In the renal subcapsular model, close proximity between hepatocytes and ADSCs was necessary to exert this effect. Unexpectedly, ≈50% of transplanted hepatocytes were attached by ADSCs in the liver. In an in vitro study, the hepatocyte function was significantly improved by ADSC coculture supernatant (P < 0.001). The multiplex assay and inhibition test demonstrated that hepatocyte growth factor, vascular endothelial growth factor, and interleukin-6 may be key factors for the abovementioned effects of ADSCs. CONCLUSIONS: The present study revealed that ADSC cotransplantation can improve the engraftment of transplanted hepatocytes. This effect may be based on crucial factors, such as hepatocyte growth factor, vascular endothelial growth factor, and interleukin-6, which are secreted by ADSCs.

  11. Short-term inhalation of isoflurane improves the outcomes of intraportal hepatocyte transplantation. International-journal

    Hiroyasu Nishimaki, Yoshikatsu Saitoh, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Ibrahim Fathi, Hiroki Yamana, Kazuo Ohashi, Shigehito Miyagi, Takashi Kamei, Michiaki Unno, Masafumi Goto

    Scientific reports　12　(1)　4241-4241　2022/03/10

    DOI： 10.1038/s41598-022-08237-6 　

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    Clinical hepatocyte transplantation (HTx) is only performed without general anesthesia, while inhalation anesthetics are usually used in animal experiments. We hypothesized that isoflurane may be a possible reason for the discrepancy between the results of animal experiments and the clinical outcomes of HTx. Syngeneic rat hepatocytes (1.0 × 107) were transplanted to analbuminemic rats with (ISO group) and without (AW group) isoflurane. The serum albumin, AST, ALT, LDH levels and several inflammatory mediators were analyzed. Immunohistochemical staining and ex vivo imaging were also performed. The serum albumin levels of the ISO group were significantly higher in comparison to the AW group (p < 0.05). The serum AST, ALT, LDH levels of the ISO group were significantly suppressed in comparison to the AW group (p < 0.0001, respectively). The serum IL-1β, IL-10, IL-18, MCP-1, RNTES, Fractalkine and LIX levels were significantly suppressed in the ISO group. The ischemic regions of the recipient livers in the ISO group tended to be smaller than the AW group; however, the distribution of transplanted hepatocytes in the liver parenchyma was comparable between the two groups. Isoflurane may at least in part be a reason for the discrepancy between the results of animal experiments and the clinical outcomes of HTx.

  12. Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures International-journal

    Takehiro Imura, Akiko Inagaki, Yasuhiro Igarashi, Masafumi Goto

    PLoS ONE　16　(8)　e0255733　2021/08/01

    Publisher: Public Library of Science

    DOI： 10.1371/journal.pone.0255733 　

    ISSN： 1932-6203

  13. The Liver Surface Is an Attractive Transplant Site for Pancreatic Islet Transplantation International-journal Peer-reviewed

    Akiko Inagaki, Takehiro Imura, Yasuhiro Nakamura, Kazuo Ohashi, Masafumi Goto

    Journal of Clinical Medicine　10　(4)　724-724　2021/02/12

    Publisher: MDPI AG

    DOI： 10.3390/jcm10040724 　

    eISSN： 2077-0383

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    In the current clinical islet transplantation, intraportal transplantation is regarded as the gold-standard procedure. However, in this procedure, 50 to 70% of the transplanted islets are immediately damaged due to a strong innate immune response based on islet–blood contact. We investigated the transplant efficiency of a novel method of liver surface transplantation using a syngeneic keratinocyte sheet to avoid islet–blood contact. To examine the influence of the keratinocyte sheet, substantial amounts of syngeneic islets (8 IEQs/g) were transplanted on the liver surface of diabetic rats, while marginal amounts of islets (4 IEQs/g) were transplanted via intraportal transplantation to compare the transplant efficiency. Blood glucose, intraperitoneal glucose tolerance, immunohistochemistry, and in vivo imaging findings of the cell sheet were evaluated. The study showed that islet transplantation to the liver surface immediately followed by a syngeneic keratinocyte sheet covering was effective for curing diabetic rats, while no rats were cured in the group without the cell sheet. Notably, islet grafts transplanted via this approach appeared to penetrate into the liver parenchyma. However, the transplant efficiency did not reach that of intraportal transplantation. Further refinements of this approach by introducing mesothelial or fibroblast cell sheets in combination with a preferable scaffold for islet grafts may help to improve the transplant efficiency.

  14. Improvement of hepatocyte engraftment by co-transplantation with pancreatic islets in hepatocyte transplantation. International-journal Peer-reviewed

    Yoshikatsu Saitoh, Akiko Inagaki, Ibrahim Fathi, Takehiro Imura, Hiroyasu Nishimaki, Hiroyuki Ogasawara, Muneyuki Matsumura, Shigehito Miyagi, Yohichi Yasunami, Michiaki Unno, Takashi Kamei, Masafumi Goto

    Journal of tissue engineering and regenerative medicine　15　(4)　361-374　2021/01/23

    DOI： 10.1002/term.3170 　

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    Because of the fragility of isolated hepatocytes, extremely poor engraftment of transplanted hepatocytes remains a severe issue in hepatocyte transplantation. Therefore, improving hepatocyte engraftment is necessary to establish hepatocyte transplantation as a standard therapy. Since the pancreatic islets are known to have favorable autocrine effects, we hypothesized that the transplanted islets might influence not only the islets but also the nearby hepatocytes, subsequently promoting engraftment. We evaluated the effects of islet co-transplantation using an analbuminemic rat model (in vivo model). Furthermore, we established a mimicking in vitro model to investigate the underlying mechanisms. In an in vivo model, the hepatocyte engraftment was significantly improved only when the islets were co-transplanted to the nearby hepatocytes (p < 0.001). Moreover, the transplanted hepatocytes appeared to penetrate the renal parenchyma together with the co-transplanted islets. In an in vitro model, the viability of cultured hepatocytes was also improved by coculture with pancreatic islets. Of particular interest, the coculture supernatant alone could also exert beneficial effects comparable to islet coculture. Although insulin, VEGF, and GLP-1 were selected as candidate crucial factors using the Bio-Plex system, beneficial effects were partially counteracted by anti-insulin receptor antibodies. In conclusion, this study demonstrated that islet co-transplantation improves hepatocyte engraftment, most likely due to continuously secreted crucial factors, such as insulin, in combination with providing favorable circumstances for hepatocyte engraftment. Further refinements of this approach, especially regarding substitutes for islets, could be a promising strategy for improving the outcomes of hepatocyte transplantation.

  15. Preferable Transplant Site for Hepatocyte Transplantation in a Rat Model International-journal

    Hiroyuki Ogasawara, Akiko Inagaki, Ibrahim Fathi, Takehiro Imura, Hiroki Yamana, Yoshikatsu Saitoh, Muneyuki Matsumura, Kengo Fukuoka, Shigehito Miyagi, Yasuhiro Nakamura, Kazuo Ohashi, Michiaki Unno, Takashi Kamei, Masafumi Goto

    Cell Transplantation　30　096368972110400-096368972110400　2021/01/01

    Publisher: SAGE Publications

    DOI： 10.1177/09636897211040012 　

    ISSN： 0963-6897

    eISSN： 1555-3892

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    Intraportal injection is regarded as the current standard procedure of hepatocyte transplantation (HTx). In islet transplantation, which shares many aspects with HTx, recent studies have clarified that instant blood-mediated inflammatory reaction (IBMIR), characterized by strong innate immune responses, can cause poor engraftment, so other transplant sites to avoid such a reaction have been established. Although IBMIR was reported to occur in HTx, few reports have evaluated alternative transplant sites for HTx. In this study, we sought to determine the optimum transplant site for HTx. Rat hepatocytes (1.0 × 107) were transplanted at the 9 transplant sites (intraportal (IPO), intrasplenic (IS), liver parenchyma, subcutaneous, intraperitoneal, renal subcapsular, muscle, inguinal subcutaneous white adipose tissue, and omentum) of analbuminemic rats. The serum albumin levels, immunohistochemical staining (albumin, TUNEL, and BrdU), and in vivo imaging of the grafts were evaluated. The serum albumin levels of the IPO group were significantly higher than those of the other groups ( p &lt; .0001). The BrdU-positive hepatocyte ratio of liver in the IS group (0.9% ± 0.2%) was comparable to that of the IPO group (0.9% ± 0.3%) and tended to be higher than that of the spleen in the IS group (0.5% ± 0.1%, p = .16). Considering the in vivo imaging evaluation and the influence of splenectomy, the graft function in the IS group may be almost entirely achieved by hepatocytes that have migrated to the liver. The present study clearly showed that the intraportal injection procedure is more efficient than other procedures for performing HTx

  16. Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis. International-journal

    Ibrahim Fathi, Takehiro Imura, Akiko Inagaki, Yasuhiro Nakamura, Ayman Nabawi, Masafumi Goto

    Frontiers in bioengineering and biotechnology　9　756755-756755　2021

    DOI： 10.3389/fbioe.2021.756755 　

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    Introduction: Whole-organ decellularization is an attractive approach for three-dimensional (3D) organ engineering. However, progress with this approach is hindered by intra-vascular blood coagulation that occurs after in vivo implantation of the re-cellularized scaffold, resulting in a short-term graft survival. In this study, we explored an alternative approach for 3D organ engineering through an axial pre-vascularization approach and examined its suitability for pancreatic islet transplantation. Methods: Whole livers from male Lewis rats were decellularized through sequential arterial perfusion of detergents. The decellularized liver scaffold was implanted into Lewis rats, and an arteriovenous bundle was passed through the scaffold. At the time of implantation, fresh bone marrow preparation (BM; n = 3), adipose-derived stem cells (ADSCs; n = 4), or HBSS (n = 4) was injected into the scaffold through the portal vein. After 5 weeks, around 2,600 islet equivalents (IEQs) were injected through the portal vein of the scaffold. The recipient rats were rendered diabetic by the injection of 65 mg/kg STZ intravenously 1 week before islet transplantation and were followed up after transplantation by measuring the blood glucose and body weight for 30 days. Intravenous glucose tolerance test was performed in the cured animals, and samples were collected for immunohistochemical (IHC) analyses. Micro-computed tomography (CT) images were obtained from one rat in each group for representation. Results: Two rats in the BM group and one in the ADSC group showed normalization of blood glucose levels, while one rat from each group showed partial correction of blood glucose levels. In contrast, no rats were cured in the HBSS group. Micro-CT showed evidence of sprouting from the arteriovenous bundle inside the scaffold. IHC analyses showed insulin-positive cells in all three groups. The number of von-Willebrand factor-positive cells in the islet region was higher in the BM and ADSC groups than in the HBSS group. The number of 5-bromo-2'-deoxyuridine-positive cells was significantly lower in the BM group than in the other two groups. Conclusions: Despite the limited numbers, the study showed the promising potential of the pre-vascularized whole-organ scaffold as a novel approach for islet transplantation. Both BM- and ADSCs-seeded scaffolds were superior to the acellular scaffold.

  17. A Simple and Useful Predictive Assay for Evaluating the Quality of Isolated Hepatocytes for Hepatocyte Transplantation. International-journal Peer-reviewed

    Muneyuki Matsumura, Takehiro Imura, Akiko Inagaki, Hiroyuki Ogasawara, Kengo Fukuoka, Ibrahim Fathi, Shigehito Miyagi, Kazuo Ohashi, Michiaki Unno, Takashi Kamei, Susumu Satomi, Masafumi Goto

    Scientific reports　9　(1)　6166-6166　2019/04/16

    DOI： 10.1038/s41598-019-42720-x 　

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    No optimal assay for assessing isolated hepatocytes before hepatocyte transplantation (HTx) has been established, therefore reliable and rapid assays are warranted. Isolated rat hepatocytes were dipped in a water bath (necrosis model), and were also cultured with Okadaic acid (apoptosis model) or vehicle, followed by cellular assessment including trypan blue exclusion (TBE) viability, ADP /ATP ratio, plating efficiency (PE), DNA quantity and ammonia elimination. Hepatocytes were transplanted into the liver of analbuminemic rats, subsequently engraftment was assessed by serum albumin and the histology of transplanted grafts. In the necrosis model, the ADP/ATP ratio was strongly and negatively correlated with the TBE (R2 = 0.559, P < 0.001). In the apoptosis model, the ADP/ATP ratio assay, PE, DNA quantification and an ammonia elimination test clearly distinguished the groups (P < 0.001, respectively). The ADP/ATP ratio, PE and DNA quantity were well-correlated and the ammonia elimination was slightly correlated with the transplant outcome. TBE could not distinguish the groups and was not correlated with the outcome. The ADP/ATP ratio assay predicted the transplant outcome. PE and DNA quantification may improve the accuracy of the retrospective (evaluations require several days) quality assessment of hepatocytes. The ADP/ATP ratio assay, alone or with a short-term metabolic assay could improve the efficiency of HTx.

  18. A Simple and Effective Non-Human Primate Diabetic Model Combining Sub-Total Pancreatectomy and Low-Dose Streptozotocin Injection

    Ibrahim Fathi, Takehiro Imura, Kozue Imura, Megumi Goto, Yasuhiro Igarashi, Akiko Inagaki, Makiko Kikkawa, Fumiko Ono, Hiroo Iwata, Yohichi Yasunami, Masafumi Goto

    OBM Transplantation　3　(3)　1-1　2019/03/27

    Publisher: LIDSEN Publishing Inc

    DOI： 10.21926/obm.transplant.1903071 　

    ISSN： 2577-5820

  19. Strategy towards tailored donor tissue-specific pancreatic islet isolation. International-journal Peer-reviewed

    Yuki Miyazaki, Kazutaka Murayama, Ibrahim Fathi, Takehiro Imura, Youhei Yamagata, Kimiko Watanabe, Hiroshi Maeda, Akiko Inagaki, Yasuhiro Igarashi, Shigehito Miyagi, Hiroki Shima, Kazuhiko Igarashi, Takashi Kamei, Michiaki Unno, Masafumi Goto

    PloS one　14　(5)　e0216136　2019

    DOI： 10.1371/journal.pone.0216136 　

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    BACKGROUND: Optimizing the collagenase G (ColG):collagenase H (ColH) ratio is a key strategy for achieving tailored donor-tissue specific islet isolation. Collagen V (Col V) and collagen III (Col III) are crucial target matrices of ColG and ColH, respectively. We herein investigated the relevance between the expression of target matrices in pancreatic tissues and influence of ColG:ColH ratio on islet isolation outcome. METHODS: Islet isolation was performed in Lewis and SD rats using different ColG:ColH ratios (5:1, 1:1 and 1:5; n = 7/group). The composition of Col III and Col V was examined using immunohistochemical staining, real-time polymerase chain reaction (PCR), Western blotting and mass spectrometry. Chain types in collagen I (Col I) were also assessed using mass spectrometry. RESULTS: No beneficial effects were observed by increasing the ColG amount, irrespective of the rat strain. In contrast, the islet yield in Lewis rats was considerably increased by high amounts of ColH but decreased in SD rats, suggesting that Lewis pancreas contains more Col III than SD pancreas. Neither immunohistochemical nor real-time PCR showed correlation with isolation outcome. However, Western blotting revealed that Lewis contained considerably higher amount of Col III than SD (p = 0.10). Likewise, Col-I(α1)/Col-III(α1) and Col-I(α2)/Col-III(α1) were significantly lower in Lewis than in SD rats (p = 0.007, respectively). Furthermore, the isolation outcome was considerably correlated with the composition of homotrimeric Col I. CONCLUSIONS: The Col III expression and the composition of homotrimeric Col I in pancreatic tissues determined using mass analyses appeared useful for optimizing the ColG:ColH ratio in islet isolation.

  20. The Optimization of the Prevascularization Procedures for Improving Subcutaneous Islet Engraftment. International-journal Peer-reviewed

    Satomi Suzuki Uematsu, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Yasuhiro Igarashi, Ibrahim Fathi, Shigehito Miyagi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    Transplantation　102　(3)　387-395　2018/03

    DOI： 10.1097/TP.0000000000001970 　

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    BACKGROUND: Subcutaneous islet transplantation is associated with minimal invasiveness, but poor vascularization. Thus, the optimization of the prevascularization procedures is crucial for improving the outcomes. Although the effectiveness of basic fibroblast growth factor (bFGF) was reported, the optimal procedures remain unclear. We sought to optimize the prevascularization procedures including the use of a novel scaffold, recombinant peptide (RCP). METHODS: Devices containing various amount of bFGF with/without heparin or RCP were implanted into the subcutaneous space of diabetic C57BL/6 mice. Syngeneic islets were transplanted into the prevascularized space. Blood glucose, intraperitoneal glucose tolerance, and immunohistochemistry were evaluated. RESULTS: The cure rates in all the device groups irrespective of bFGF doses were considerably higher than in the nondevice group. The cure rate in the bFGF0 group was unexpectedly higher than that in the subcutaneous islet transplant alone group (the None group) (57.1% vs 28.6%). Glucose tolerance was ameliorated in the bFGF10(-), 10(+) and 15(-) groups. The number of von Willebrand factor-positive vessels in the bFGF10(+) group was significantly higher than that in the None and bFGF0 groups (P < 0.01). Taken together, the bFGF10(+) group was considered to have received optimized procedures. In a marginal graft model, the efficiency in the RCP group was better than that in the bFGF10(+) group, furthermore, comparable to that in the intraportal transplantation group. Unlike bFGF, no bleeding and effusion were observed in the RCP group. CONCLUSIONS: These results suggest that optimizing biomaterials to induce efficient prevascularization could be a novel strategy for improving subcutaneous islet transplantation.

  21. The Optimization of Short-Term Hepatocyte Preservation Before Transplantation. International-journal Peer-reviewed

    Kengo Fukuoka, Akiko Inagaki, Yasuhiro Nakamura, Muneyuki Matsumura, Satoru Yoshida, Takehiro Imura, Yasuhiro Igarashi, Shigehito Miyagi, Kazuo Ohashi, Shin Enosawa, Takashi Kamei, Michiaki Unno, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    Transplantation direct　3　(7)　e176　2017/07

    DOI： 10.1097/TXD.0000000000000687 　

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    BACKGROUND: No optimal methods for short-term hepatocyte preservation have been established. We have recently developed a prominent oxygen-permeable bag (Tohoku Device [TD]) for pancreatic islet culture and transplantation. In this study, we investigated whether TD is also effective for hepatocyte preservation and tried to optimize other conditions. METHODS: Hepatocytes were preserved in the following conditions, and their outcomes were observed. First, the effectiveness of TD was investigated. Second, hepatocyte medium (HM) and organ preservation solutions with or without fetal bovine serum (FBS) were compared. Third, as supplementations, FBS and human serum albumin (HSA) were compared. Fourth, low, room and high temperature were compared. And finally, hepatocytes preserved in various conditions were transplanted into the subrenal capsule space of nonalbumin rats and engrafted areas were assessed. RESULTS: The survival rate of hepatocytes preserved in TD tended to be higher and their viability and function were maintained significantly greater than those of non-TD group. Irrespective of FBS supplementation, the survival rate of HM group was significantly higher than those of organ preservation solution group while viabilities and plating efficiency were similar among them. Although survival rates of groups without FBS were extremely low, results of HSA supplemented group were not inferior to FBS supplemented group. Hepatocytes preserved at high temperature had the worst results. The engrafted area of TD group tended to be higher than those of other groups. CONCLUSIONS: TD is effective for short-term hepatocyte preservation. HSA is a useful substitute for FBS, and preserving in HM at low temperature is recommended.

  22. Collagen V Is a Potential Substrate for Clostridial Collagenase G in Pancreatic Islet Isolation. International-journal Peer-reviewed

    Hiroki Shima, Akiko Inagaki, Takehiro Imura, Youhei Yamagata, Kimiko Watanabe, Kazuhiko Igarashi, Masafumi Goto, Kazutaka Murayama

    Journal of diabetes research　2016　4396756-4396756　2016

    DOI： 10.1155/2016/4396756 　

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    The clostridial collagenases, H and G, play key roles in pancreatic islet isolation. Collagenases digest the peptide bond between Yaa and the subsequent Gly in Gly-Xaa-Yaa repeats. To fully understand the pancreatic islet isolation process, identification of the collagenase substrates in the tissue is very important. Although collagen types I and III were reported as possible substrates for collagenase H, the substrate for collagenase G remains unknown. In this study, collagen type V was focused upon as the target for collagenases. In vitro digestion experiments for collagen type V were performed and analyzed by SDS-PAGE and mass spectrometry. Porcine pancreatic tissues were digested in vitro under three conditions and observed during digestion. The results revealed that collagen type V was only digested by collagenase G and that the digestion was initiated from the N-terminal part. Tissue degradation during porcine islet isolation was only observed in the presence of both collagenases H and G. These findings suggest that collagen type V is one of the substrates for collagenase G. The enzymatic activity of collagenase G appears to be more important for pancreatic islet isolation in large mammals such as pigs and humans.

  23. Synergistic Effect of Neutral Protease and Clostripain on Rat Pancreatic Islet Isolation. International-journal Peer-reviewed

    Mami Dendo, Hiroshi Maeda, Youhei Yamagata, Kazutaka Murayama, Kimiko Watanabe, Takehiro Imura, Akiko Inagaki, Yasuhiro Igarashi, Yasutake Katoh, Masayuki Ebina, Keisei Fujimori, Kazuhiko Igarashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    Transplantation　99　(7)　1349-55　2015/07

    DOI： 10.1097/TP.0000000000000662 　

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    BACKGROUND: Islet isolation currently requires collagenase, neutral protease and other components. Thermolysin (TL) from Bacillus thermoproteolyticus is the gold standard neutral protease. However, we speculated that neutral protease derived from Clostridium histolyticum (Ch; ChNP) would be biologically superior for islet isolation. Tryptic-like activity has also been reported to be important. Therefore, we focused on clostripain (CP), since it is one of the main proteases in Clostridium histolyticum which possesses tryptic-like activity. We then examined the synergistic effects of highly purified ChNP and CP on rat islet isolation. METHODS: The same amount of collagenase was used in all four groups (TL, ChNP, TL+CP and ChNP+CP; n = 12/group). The efficiency was evaluated by the islet yield and function. An immunohistochemical analysis, in vitro digestion assay for each enzyme component and evaluation of the activation of endogenous exocrine proteases during islet isolation were also performed. RESULTS: The islet yield of the TL group was significantly higher than that of the ChNP group (P < 0.01). The islet yield was dose dependently increased in the ChNP+CP group, but was decreased in the TL + CP group. The islet yield in the ChNP + CP group was significantly higher than that in the TL group, but their islet function was similar. Different specificities for laminin, especially laminin-511, were observed in the TL, ChNP, and CP groups. CONCLUSIONS: Clostripain had a strong synergistic effect with ChNP, but not with TL. Therefore, ChNP and CP, in combination with collagenase derived from the same bacteria, may effectively increase the isolation efficiency without affecting the quality of islets.

  24. The Influence of Collagen III Expression on the Efficiency of Cell Isolation With the Use of Collagenase H Peer-reviewed

    S. Yoshida, Y. Yamagata, K. Murayama, K. Watanabe, T. Imura, Y. Igarashi, A. Inagaki, K. Fujimori, K. Ohashi, N. Ohuchi, S. Satomi, M. Goto

    TRANSPLANTATION PROCEEDINGS　46　(6)　1942-1944　2014/07

    DOI： 10.1016/j.transproceed.2014.06.007 　

    ISSN： 0041-1345

    eISSN： 1873-2623

  25. A novel resting strategy for improving islet engraftment in the liver. International-journal Peer-reviewed

    Takuya Jimbo, Akiko Inagaki, Takehiro Imura, Satoshi Sekiguchi, Yasuhiro Nakamura, Keisei Fujimori, Jun-ichiro Miyagawa, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    Transplantation　97　(3)　280-6　2014/02/15

    DOI： 10.1097/01.tp.0000437557.50261.b9 　

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    BACKGROUND: Several studies have revealed that posttransplant insulin treatment is beneficial to rest the islet grafts. However, insulin infusion per se is not enough to completely suppress the heavy workload arising caused by postprandial hyperglycemia. Therefore, the present study examined whether short-term fasting combined with insulin treatment could effectively prevent graft exhaustion after intraportal islet transplantation. METHODS: A marginal dose of syngeneic rat islet grafts were transplanted intraportally into the control, insulin-treated, and insulin+rest groups of streptozotocin-induced diabetic rats. The control group fed freely without insulin treatment, and the other groups were continuously treated with an optimal amount of insulin to maintain normoglycemia. In addition, the insulin+rest group fasted and received total parenteral nutrition during the 2 weeks after transplantation. RESULTS: The curative rate was significantly higher in both the insulin and insulin+rest groups than the control group (P<0.0001). The glucose tolerance, residual graft mass, and graft function were significantly ameliorated in the insulin+rest group, but not in the insulin group, compared to the control group (P<0.01, P=0.03, P=0.001). CONCLUSIONS: These data suggest that short-term fasting combined with insulin treatment, especially during the avascular period of the grafts, could therefore be a promising regimen for improving pancreatic islet engraftment in the liver.

  26. Collagenase H is crucial for isolation of rat pancreatic islets. International-journal Peer-reviewed

    Atsushi Fujio, Kazutaka Murayama, Youhei Yamagata, Kimiko Watanabe, Takehiro Imura, Akiko Inagaki, Naomi Ohbayashi, Hiroki Shima, Satoshi Sekiguchi, Keisei Fujimori, Kazuhiko Igarashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    Cell transplantation　23　(10)　1187-98　2014

    DOI： 10.3727/096368913X668654 　

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    The role(s) of collagenase G (ColG) and collagenase H (ColH) during pancreatic islet isolation remains controversial, possibly due to the enzyme blends used in the previous studies. We herein examined the role of ColG and ColH using highly pure enzyme blends of recombinant collagenase of each subtype. Rat pancreases were digested using thermolysin, together with ColG, ColH, or ColG/ColH (n = 9, respectively). No tryptic-like activity was detected in any components of the enzyme blends. The efficiency of the collagenase subtypes was evaluated by islet yield and function. Immunohistochemical analysis, in vitro collagen digestion assay, and mass spectrometry were also performed to examine the target matrix components of the crucial collagenase subtype. The islet yield was highest in the ColG/ColH group (4,101 ± 460 islet equivalents). A substantial number of functional islets (2,811 ± 581 islet equivalents) was obtained in the ColH group, whereas no islets were retrieved in the ColG group. Mass spectrometry demonstrated that ColH reacts with collagen I and III. In the immunohistochemical analysis, both collagen I and III were located in exocrine tissues, although collagen III expression was more pronounced. The collagen digestion assay showed that collagen III was more effectively digested by ColH than by ColG. The present study reveals that ColH is crucial, while ColG plays only a supporting role, in rat islet isolation. In addition, collagen III appears to be one of the key targets of ColH.

  27. （Secondary Publication）Ductal injection does not increase the islet yield or function after cold storage in a vascular perfusion model

    NAKANISHI Wataru, IMURA Takehiro, INAGAKI Akiko, NAKAMURA Yasuhiro, SEKIGUCHI Satoshi, FUJIMORI Keisei, OHUCHI Noriaki, SATOMI Susumu, GOTO Masafumi

    Japanese Journal of Transplantation　48　(6)　405-416　2013

    Publisher: The Japan Society for Transplantation

    DOI： 10.11386/jst.48.405 　

    ISSN： 0578-7947

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    Several studies have reported that pancreatic ductal preservation greatly improved the islet yield and function after cold storage. However, these studies were devoid of appropriate controls, such as vascular perfusion, which is routinely performed to preserve organs in the clinical setting. In this study, we created a vascular perfusion model using inbred rats, and investigated the effects of ductal injection on the islet yield and function after cold storage. Rat pancreases after 10 h cold ischemia were classified as follows: without ductal/vascular perfusion; with ductal injection; with vascular perfusion; and with ductal/vascular perfusion. The islet yield, function, viability, release of inflammatory mediators, and pathological changes in the exocrine tissues were assessed in the Hanks Balanced Salt Solution model. The islet yield was also assessed by introducing University of Wisconsin Solution and Histidine-Tryptophan-Ketoglutarate Solution, which are the standard clinical preservation solutions. In the Hanks Balanced Salt Solution model, ductal injection and vascular perfusion significantly improved the islet yield compared with the control group. However, ductal injection showed no additional effects on the islet yield, function, viability, and suppression of the release of inflammatory mediators when vascular perfusion was performed. Although ductal injection significantly decreased the apoptosis of exocrine cells, no beneficial effect on vacuolation was observed. In contrast, vascular perfusion significantly suppressed vacuolation in the exocrine tissues. Likewise, in the University of Wisconsin Solution and Histidine-Tryptophan-Ketoglutarate Solution model, ductal injection and vascular perfusion improved the islet yield compared with the control group. Nevertheless, the combination group showed no additional effects. These data suggest that ductal injection has no additional effect on islet yield and function after cold storage in a vascular perfusion model. We propose that ductal injection can be an effective and simple alternative for vascular perfusion prior to pancreas harvest, but it is usually unnecessary, since vascular perfusion is routinely performed.<br/>This article is based on a study first reported in [Nakanishi W, Imura T, Inagaki A, et al. Ductal injection does not increase the islet yield or function after cold storage in a vascular perfusion model. PloS one 2012; 7: e42319.].

  28. Thioredoxin-1 attenuates early graft loss after intraportal islet transplantation in mice. International-journal Peer-reviewed

    Kengo Asami, Akiko Inagaki, Takehiro Imura, Satoshi Sekiguchi, Keisei Fujimori, Hiroshi Masutani, Junji Yodoi, Susumu Satomi, Noriaki Ohuchi, Masafumi Goto

    PloS one　8　(8)　e70259　2013

    DOI： 10.1371/journal.pone.0070259 　

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    AIMS: Recent studies suggest that decreasing oxidative stress is crucial to achieve successful islet transplantation. Thioredoxin-1 (TRX), which is a multifunctional redox-active protein, has been reported to suppress oxidative stress. Furthermore, it also has anti-inflammatory and anti-apoptotic effects. In this study, we investigated the effects of TRX on early graft loss after islet transplantation. METHODS: Intraportal islet transplantation was performed for two groups of streptozotocin-induced diabetic mice: a control and a TRX group. In addition, TRX-transgenic (Tg) mice were alternately used as islet donors or recipients. RESULTS: The changes in blood glucose levels were significantly lower in the TRX group compared with the TRX-Tg donor and control groups (p<0.01). Glucose tolerance and the residual graft mass were considerably better in the TRX group. TRX significantly suppressed the serum levels of interleukin-1β (p<0.05), although neither anti-apoptotic nor anti-chemotactic effects were observed. Notably, no increase in the 8-hydroxy-2'-deoxyguanosine level was observed after islet infusion, irrespective of TRX administration. CONCLUSIONS: The present study demonstrates that overexpression of TRX on the islet grafts is not sufficient to improve engraftment. In contrast, TRX administration to the recipients exerts protective effects on transplanted islet grafts by suppressing the serum levels of interleukin-1β. However, TRX alone appears to be insufficient to completely prevent early graft loss after islet transplantation. We therefore propose that a combination of TRX and other anti-inflammatory treatments represents a promising regimen for improving the efficacy of islet transplantation.

  29. Ductal injection does not increase the islet yield or function after cold storage in a vascular perfusion model. International-journal Peer-reviewed

    Wataru Nakanishi, Takehiro Imura, Akiko Inagaki, Yasuhiro Nakamura, Satoshi Sekiguchi, Keisei Fujimori, Susumu Satomi, Masafumi Goto

    PloS one　7　(8)　e42319　2012

    DOI： 10.1371/journal.pone.0042319 　

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    Several studies have reported that pancreatic ductal preservation greatly improved the islet yield and function after cold storage. However, these studies were devoid of appropriate controls, such as vascular perfusion, which is routinely performed to preserve organs in the clinical setting. In this study, we created a vascular perfusion model using inbred rats, and investigated the effect of ductal injection on the islet yield and function after cold storage. Rat pancreases after 10 h cold ischemia were classified as follows: without ductal/vascular perfusion; with ductal injection; with vascular perfusion; and with ductal/vascular perfusion. The islet yield, function, viability, release of inflammatory mediators, and pathological changes in the exocrine tissues were assessed in the Hanks' Balanced Salt Solution (HBSS) model. The islet yield was also assesed by introducing University of Wisconsin Solution (UWS) and Histidine-Tryptophan-Ketoglutarate solution (HTK), which are the standard clinical preservation solutions. In the HBSS model, ductal injection and vascular perfusion significantly improved the islet yield compared with the control group. However, ductal injection showed no additional effects on the islet yield, function, viability and suppressing the release of inflammatory mediators when vascular perfusion was performed. Although ductal injection significantly decreased the apoptosis of exocrine cells, no beneficial effect on vacuolation was observed. In contrast, vascular perfusion significantly suppressed vacuolation in the exocrine tissues. Likewise, in the UWS and HTK model, ductal injection and vascular perfusion improved the islet yield compared with the control group. Nevertheless, the combination group showed no additional effects. These data suggest that ductal injection has no additional effect on islet yield and function after cold storage in a vascular perfusion model. We propose that ductal injection can be an effective and simple alternative for vascular perfusion prior to pancreas harvest, but is not necessary in most cases, since vascular perfusion is routinely performed.

  30. Attenuation of cross-talk between the complement and coagulation cascades by C5a blockade improves early outcomes after intraportal islet transplantation. International-journal Peer-reviewed

    Kazuaki Tokodai, Masafumi Goto, Akiko Inagaki, Wataru Nakanishi, Norihiko Ogawa, Kazushige Satoh, Naoki Kawagishi, Satoshi Sekiguchi, Bo Nilsson, Noriko Okada, Hidechika Okada, Susumu Satomi

    Transplantation　90　(12)　1358-65　2010/12/27

    eISSN： 1534-6080

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    BACKGROUND: Complement 5a factor (C5a) elicits a broad range of proinflammatory effects, including chemotaxis of inflammatory cells and cytokine release. C5a is also linked to the coagulant activity in autoimmune diseases. Therefore, C5a most likely plays a crucial role in the instant blood-mediated inflammatory reaction. METHODS: Intraportal transplantation of 2.5 islet equivalents/g of syngeneic rat islet grafts was performed in two groups of streptozotocin-induced diabetic rats: controls and C5a inhibitory peptide (C5aIP)-treated group. RESULTS: The thrombin-antithrombin complex was significantly suppressed in the C5aIP group (P=0.003), and both the curative rate and the glucose tolerance were significantly improved in the C5aIP group (P<0.05 and P<0.005, respectively). Expression of tissue factor on granulocytes in recipient livers was up-regulated 1 h after islet infusion (P<0.0001), which was significantly suppressed by C5aIP (P<0.005). However, C5aIP was unable to regulate tissue factor expression on isolated islets. Furthermore, no differences were detected between the groups, regarding infiltration of CD11b-positive cells and deposition of C5b-9 on the islet grafts. CONCLUSIONS: These data suggest that C5aIP attenuates cross-talk between the complement and coagulation cascades through suppressing up-regulation of tissue factor expression on leukocytes in recipient livers but not on islet grafts, a process leading to improvement in islet engraftment. Therefore, C5aIP in combination with conventional anticoagulants could be a strong candidate strategy to control the instant blood-mediated inflammatory reaction induced in clinical islet transplantation.

  31. Evaluation of a poly(L-lactic acid) stent for sutureless vascular anastomosis. International-journal

    Yoshiyuki Nakano, Yoshio Hori, Akira Sato, Tetsuo Watanabe, Shuji Takada, Hitoshi Goto, Akiko Inagaki, Yoshito Ikada, Susumu Satomi

    Annals of vascular surgery　23　(2)　231-8　2009/03

    DOI： 10.1016/j.avsg.2008.07.004 　

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    We have developed a sutureless anastomosis device consisting of a biodegradable stent and stainless steel band for end-to-end anastomosis. The aim of this acute phase study was to evaluate the feasibility of a sutureless anastomotic procedure with a bioabsorbable stent during a 4-week period in a swine model. The porcine infrarenal aorta was replaced with an expanded polytetrafluoroethylene graft. A proximal anastomosis was completed using a sutureless anastomotic procedure employing a bioabsorbable stent made of poly(L-lactic acid) (PLLA) and a stainless steel plate. A distal anastomosis completed by manual suturing served as a control. At 4 weeks after surgery, angiography was performed. The animals were then killed, and the specimens were evaluated histologically. The sutureless anastomotic procedure required significantly less time than the suturing technique. Angiograms showed patency of the grafts, and no signs of either stenosis or leakage. Both pressure-proof and tensile tests confirmed the adequate mechanical strength of the anastomoses. Sutureless anastomosis with a PLLA stent appears to be feasible, at least within an observation period of 4 weeks. This simple procedure shortened the time of surgery and would contribute to reducing the risks of operation-related complications.

  32. Inhibitory effect of succinic acid on epithelial cell proliferation of colonic mucosa in rats. Peer-reviewed

    Akiko Inagaki, Hirofumi Ichikawa, Takashi Sakata

    Journal of nutritional science and vitaminology　53　(4)　377-9　2007/08

    ISSN： 0301-4800

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    Microbial breakdown of carbohydrates in the large intestine mainly produces short-chain fatty acids (SCFA). SCFA stimulate epithelial cell proliferation of the digestive tract in vivo. Succinic acid sometimes accumulates in the colonic lumen. However, the effect of succinic acid on colonic epithelial cell proliferation is unknown. Thus, we planned to clarify the influence of succinic acid on colonic epithelial cell proliferation in vivo. We continuously administered infusate with or without succinic acid (100 mM) into the distal colon of rats for 6 d and measured accumulated mitosis per crypt of distal colon of these rats. Succinic acid infused into rat colons significantly inhibited colonic cell proliferation and reduced crypt size. These results clearly indicated the inhibitory effects of succinic acid on colonic epithelial cell proliferation in vivo.

  33. Dose-dependent stimulatory and inhibitory effects of luminal and serosal n-butyric acid on epithelial cell proliferation of pig distal colonic mucosa. Peer-reviewed

    Akiko Inagaki, Takashi Sakata

    Journal of nutritional science and vitaminology　51　(3)　156-60　2005/06

    ISSN： 0301-4800

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    Large bowel bacteria convert various carbohydrates into short-chain fatty acids (SCFA). SCFA stimulate epithelial cell proliferation of the large intestine in vivo and inhibit that of various cells in vitro. Supposing that too high concentration of SCFA on the serosal side is responsible for their inhibitory effect in vitro, we studied effects of luminal and serosal n-butyric acid (0, 0.1, 1, or 10 mmol/L, adjusted to neutral pH) on the epithelial cell proliferation rate of pig colonic mucosa in organ culture taking crypt cell production rate (CCPR) as the measure of proliferative activity. With 0 or 0.1 mmol/L n-butyric acid on the serosal side, luminal n-butyric acid increased CCPR at 1.0 mmol/L, and decreased CCPR at 10 mmol/L when compared to the luminal 0 mmol/L control. With 1.0 or 10 mmol/L serosal n-butyric acid, luminal n-butyric acid depressed CCPR dose-dependently. The above results indicated that n-butyric acid stimulated colonic epithelial cell proliferation at low concentration and inhibit it at high concentration with interaction effect to enhance the inhibitory action. The stimulatory effect of a low dose of serosal n-butyric acid may be responsible for the distant trophic effect of SCFA.

  34. Effect of the Viscous Exudate of Mekabu (Sporophyll of Undaria pinnatifida) on the Rate of Cecal Fermentation in Rats Peer-reviewed

    YAMANAKA Natsumi, INAGAKI Akiko, SAKATA Takashi, OGAWA Noriko

    Journal of home economics of Japan　53　(10)　991-999　2002

    Publisher:

    DOI： 10.11428/jhej1987.53.991 　

    ISSN： 0913-5227 1882-0352

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    The suppressive effect of ingesting a viscous exudate of mekabu on the rate of cecal fermentation in rats was evaluated from the lactic acid concentration in the cecum. Powdered feed including 7.5% fructooligosaccharides and 10% freeze-dried viscous exudate of mekabu were mixed with twice the amount of water to form a paste, and this was fed to male Wistar rats for four days. Feeding was limited 3 h after the start of the dark period. On the final day, the rats were sacrificed either 3 or 6 h after feeding, and the tissue weight of the gastrointestinal segments, the weight of the gastro-intestinal lumen contents, the viscosity of the cecal contents and the cecal organic acid concentration were measured. The retention time of the contents in various segments of the gastro-intestinal tract was estimated from the weight of the gastro-intestinal lumen contents. The retention time in the stomach and small intestine was prolonged after the ingestion of the viscous exudate of mekabu. This effect is thought to have been dependent on the viscosity of the contents. The lactic acid concentration in the cecal contents of the rats that had consumed a diet containing a viscous exudate of mekabu was lower than that in rats that had consumed a diet without the additive. This indicates that consumption of the viscous exudate suppressed the rate of fermentation by the cecal bacteria. Ingestion of the viscous exudate of mekabu increased the viscosity of the gastro-intestinal lumen contents and decreased the rate at which the substrate flowed into the cecum. At the same time, the metabolism of the intestinal bacteria was inhibited by the flow into the cecum of the low-fermentability viscous substances themselves.

  35. Probiotic bacteria stimulate gut epithelial cell proliferation in rat Peer-reviewed

    H Ichikawa, T Kuroiwa, A Inagaki, R Shineha, T Nishihira, S Satomi, T Sakata

    DIGESTIVE DISEASES AND SCIENCES　44　(10)　2119-2123　1999/10

    DOI： 10.1023/A:1026647024077 　

    ISSN： 0163-2116

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Misc. 144

  1. 膵島移植においてアロ免疫拒絶制御以外に別の膵島生着促進作用も併せ持つ新規免疫抑制薬の開発

    鈴木 翔輝, 猪村 武弘, 稲垣 明子, 前川 正充, 奥平 貴成, 片野 匠, 公文代 將希, 佐藤 裕, 戸子台 和哲, 海野 倫明, 亀井 尚, 渡邉 君子, 後藤 昌史

    Organ Biology　31　(3)　106-106　2024/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  2. 膵臓移植・膵島移植・人工膵臓の現状と展望 皮下膵島移植における低酸素環境改善が移植膵島グラフトに与える影響

    三頭 啓明, 猪村 武弘, 稲垣 明子, 鈴木 翔輝, 遠藤 有希子, 片野 匠, 戸子台 和哲, 渡邉 君子, 亀井 尚, 海野 倫明, 後藤 昌史

    膵臓　39　(3)　A279-A279　2024/07

    Publisher: (一社)日本膵臓学会

    ISSN： 0913-0071

    eISSN： 1881-2805

  3. 膵島移植に理想的な新規免疫抑制プロトコールの開発

    鈴木翔輝, 猪村武弘, 前川正充, 稲垣明子, 眞屋梢, 後藤めぐみ, 遠藤有希子, 片野匠, 戸子台和哲, 戸子台和哲, 海野倫明, 亀井尚, 後藤昌史

    日本膵・膵島移植学会プログラム・抄録集　51st　2024

  4. 不織布構造ゼラチン基材を用いた新規膵島移植法の構築

    遠藤有希子, 稲垣明子, 中村保宏, 猪村武弘, 片野匠, 鈴木翔輝, 戸子台和哲, 亀井尚, 海野倫明, 渡邉君子, 田畑泰彦, 後藤昌史

    日本膵・膵島移植学会プログラム・抄録集　51st　2024

  5. Development of a novel islet transplantation procedure by introducing a gelatin hydrogel nonwoven fabric

    後藤昌史, 稲垣明子, 中村保宏, 猪村武弘, 金井哲史, 斎藤竜助, 遠藤有希子, 片野匠, 田畑泰彦, 渡邉君子

    日本再生医療学会総会(Web)　23rd　2024

  6. 皮下膵島移植における低酸素環境改善が移植膵島グラフトに与える影響

    三頭啓明, 猪村武弘, 稲垣明子, 鈴木翔輝, 遠藤有希子, 片野匠, 戸子台和哲, 渡邉君子, 亀井尚, 海野倫明, 後藤昌史, 後藤昌史

    膵臓(Web)　39　(3)　2024

    ISSN： 1881-2805

  7. 新規肝細胞移植法の構築

    片野匠, 稲垣明子, 猪村武弘, 山名浩樹, 齊藤竜助, 遠藤有希子, 鈴木翔輝, 萩原義也, 大橋一夫, 渡邉君子, 田畑泰彦, 後藤昌史, 後藤昌史

    日本組織移植学会雑誌　22　(1)　2024

    ISSN： 1347-6491

  8. 肝表面への肝細胞移植は経門脈的アプローチに代わる移植方法となりうるか?

    片野 匠, 稲垣 明子, 猪村 武弘, 後藤 昌史

    Organ Biology　30　(3)　112-112　2023/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  9. 不織布構造ゼラチン基材を用いた肝表面膵島移植法の検討

    遠藤 有希子, 稲垣 明子, 猪村 武弘, 鈴木 翔輝, 片野 匠, 戸子台 和哲, 亀井 尚, 海野 倫明, 田畑 泰彦, 後藤 昌史

    移植　58　(総会臨時)　292-292　2023/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  10. 不織布構造ゼラチン基材と脂肪由来幹細胞の併用による皮下膵島移植成績の検討

    齊藤竜助, 稲垣明子, 中村保宏, 猪村武弘, 片野匠, 鈴木翔輝, 遠藤有希子, 三頭啓明, 金井哲史, 戸子台和哲, 亀井尚, 海野倫明, 渡邉君子, 田畑泰彦, 後藤昌史

    日本膵・膵島移植学会プログラム・抄録集　50th (CD-ROM)　2023

  11. 不織布構造ゼラチン基材の前留置期間が皮下膵島移植のグラフト生着へ及ぼす影響に関する検討

    齊藤竜助, 稲垣明子, 中村保宏, 猪村武弘, 片野匠, 鈴木翔輝, 遠藤有希子, 三頭啓明, 金井哲史, 戸子台和哲, 亀井尚, 海野倫明, 渡邉君子, 田畑泰彦, 後藤昌史

    日本膵・膵島移植学会プログラム・抄録集　50th (CD-ROM)　2023

  12. 低酸素環境改善を目的とした新規組織内酸素濃度測定法の開発と皮下膵島移植への応用

    三頭啓明, 猪村武弘, 稲垣明子, 鈴木翔輝, 遠藤有希子, 片野匠, 齋藤竜助, 戸子台和哲, 渡邉君子, 亀井尚, 海野倫明, 後藤昌史, 後藤昌史

    日本膵・膵島移植学会プログラム・抄録集　50th (CD-ROM)　2023

  13. 肝表面への肝細胞移植は経門脈的アプローチに代わる移植方法となりうるか?

    片野匠, 稲垣明子, 猪村武弘, 後藤昌史

    Organ Biology (Web)　30　(3)　2023

    ISSN： 2188-0204

  14. 皮下における膵島生着環境の創出

    稲垣明子, 齊藤竜助, 金井哲史, 植松智海, 中村保宏, 猪村武弘, 三頭啓明, 遠藤有希子, 片野匠, 鈴木翔輝, 渡邉君子, 田畑泰彦, 後藤昌史

    日本再生医療学会総会(Web)　22nd　2023

  15. ラット皮下膵島移植における不織布構造ゼラチン基材の前留置が移植結果へ及ぼす影響

    齊藤 竜助, 稲垣 明子, 中村 保宏, 猪村 武弘, 片野 匠, 戸子台 和哲, 亀井 尚, 海野 倫明, 田畑 泰彦, 後藤 昌史

    Organ Biology　29　(3)　125-125　2022/11

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  16. 皮下膵島移植における低酸素環境改善のための新規組織内酸素濃度測定法の開発

    三頭 啓明, 猪村 武弘, 稲垣 明子, 遠藤 有希子, 片野 匠, 齋藤 竜助, 宮城 重人, 渡邉 君子, 亀井 尚, 海野 倫明, 後藤 昌史

    移植　57　(総会臨時)　312-312　2022/10

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  17. 皮下膵島移植における不織布構造ゼラチン基材の前留置期間に関する検討

    齊藤竜助, 稲垣明子, 中村保宏, 猪村武弘, 遠藤有希子, 三頭啓明, 金井哲史, 宮城重人, 亀井尚, 海野倫明, 田畑泰彦, 後藤昌史

    日本膵・膵島移植学会プログラム・抄録集　49th　2022

  18. 肝細胞と脂肪由来幹細胞の共移植による移植肝細胞生着促進効果

    山名浩樹, 稲垣明子, 猪村武弘, 中村保宏, 西牧宏泰, 片野匠, 大橋一夫, 宮城重人, 亀井尚, 海野倫明, 後藤昌史, 後藤昌史

    日本移植学会総会プログラム抄録集　58th (Web)　(総会臨時)　308-308　2022

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  19. 不織布構造ゼラチン基材と脂肪由来幹細胞の併用による皮下膵島移植の成績向上

    齊藤竜助, 稲垣明子, 中村保宏, 猪村武弘, 片野匠, 遠藤有希子, 三頭啓明, 金井哲史, 宮城重人, 亀井尚, 海野倫明, 田畑泰彦, 後藤昌史

    日本移植学会総会プログラム抄録集　58th (Web)　(総会臨時)　312-312　2022

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  20. 経門脈移植に代わる膵島移植法の確立

    稲垣明子, 金井哲史, 中村保宏, 猪村武弘, 三頭啓明, 齊藤竜助, 遠藤有希子, 田畑泰彦, 大橋一夫, 後藤昌史

    日本再生医療学会総会(Web)　21st　2022

  21. 吸入麻酔薬、イソフルランによる肝細胞移植グラフト生着促進効果に関する検討

    西牧 宏泰, 齋藤 純健, 稲垣 明子, 中村 保宏, 山名 浩樹, 猪村 武弘, 大橋 一夫, 宮城 重人, 亀井 尚, 海野 倫明, 後藤 昌史

    移植　56　(総会臨時)　O4-2　2021/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  22. 不織布構造ゼラチン基材の導入による皮下膵島移植法の確立

    金井 哲史, 稲垣 明子, 中村 保宏, 猪村 武弘, Fathi Ibrahim, 三頭 啓明, 齋藤 竜助, 宮城 重人, 亀井 尚, 海野 倫明, 田畑 泰彦, 後藤 昌史

    移植　56　(総会臨時)　P2-21　2021/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  23. イソフランが肝細胞移植においてグラフト生着へ及ぼす影響に関する検討

    西牧 宏泰, 齋藤 純健, 稲垣 明子, 中村 保宏, 山名 浩樹, 猪村 武弘, 大橋 一夫, 宮城 重人, 亀井 尚, 海野 倫明, 後藤 昌史

    日本組織移植学会雑誌　19　(1)　66-66　2021/07

    Publisher: (一社)日本組織移植学会

    ISSN： 1347-6491

  24. 不織布構造ゼラチン基材の導入による経門脈移植を凌駕する革新的皮下膵島移植法の確立

    金井哲史, 稲垣明子, 中村保宏, 猪村武弘, FATHI Ibrahim, 三頭啓明, 斎藤竜助, 宮城重人, 亀井尚, 海野倫明, 田畑泰彦, 後藤昌史

    日本膵・膵島移植研究会プログラム・抄録集　48th　2021

  25. 皮下膵島移植成績向上の基盤となる新規組織内酸素濃度測定方法の開発

    三頭 啓明, 猪村 武弘, 稲垣 明子, Fathi Ibrahim, 宮城 重人, 亀井 尚, 海野 倫明, 後藤 昌史

    移植　55　(総会臨時)　349-349　2020/10

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  26. 膵島移植におけるKRP-203の有用性に関する検証

    Ibrahim Fathi, 西村 隆一, 猪村 武弘, 稲垣 明子, 牛山 明, 山口 浩明, 後藤 昌史

    Organ Biology　26　(3)　83-83　2019/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  27. 肝細胞移植の成績向上に寄与するグラフト生着促進因子の同定および機序の検討

    齋藤 純健, 稲垣 明子, 猪村 武弘, 後藤 めぐみ, Ibrahim Fathi, 西牧 宏泰, 小笠原 弘之, 宮城 重人, 海野 倫明, 亀井 尚, 安波 洋一, 後藤 昌史

    Organ Biology　26　(3)　119-119　2019/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  28. 膵島移植におけるKRP-203の有用性に関する検証

    Ibrahim Fathi, 西村 隆一, 猪村 武弘, 稲垣 明子, 牛山 明, 山口 浩明, 後藤 昌史

    Organ Biology　26　(3)　83-83　2019/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  29. 膵島移植の現況と今後の方向性 膵島移植と再生医療の融合を促進する細胞デバイスの開発

    後藤 昌史, 稲垣 明子, 猪村 武弘, Ibrahim Fathi, 水井 崇浩, 金井 哲史, 宮城 重人, 村山 和隆

    移植　54　(総会臨時)　172-172　2019/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  30. 膵島移植におけるKRP-203の有用性に関する検証

    ファテヒ・イブラヒム, 西村 隆一, 猪村 武弘, 稲垣 明子, 牛山 明, 山口 浩明, 後藤 昌史

    移植　54　(総会臨時)　232-232　2019/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  31. 免疫抑制剤不要の次世代膵島移植実現へ向けた試み

    後藤昌史, 後藤昌史, 稲垣明子, 猪村武弘, IBRAHIM Fathi, 水井崇浩, 金井哲史, 戸子台和哲, 宮城重人, 村山和隆, 亀井尚, 海野倫明

    日本膵・膵島移植研究会プログラム・抄録集　46th　2019

  32. 脂肪由来幹細胞を付加したリコンビナントペプチドの導入による新規皮下膵島移植法の開発

    水井崇浩, 稲垣明子, 中村保宏, IBRAHIM Fathi, 猪村武弘, 植松智海, 宮城重人, 亀井尚, 海野倫明, 後藤昌史, 後藤昌史

    日本膵・膵島移植研究会プログラム・抄録集　46th　2019

  33. 肝細胞移植における至適移植部位の検討

    小笠原 弘之, 稲垣 明子, 猪村 武弘, 齋藤 純健, 松村 宗幸, 福岡 健吾, Fathi Ibrahim, 宮城 重人, 大橋 一夫, 海野 倫明, 亀井 尚, 後藤 昌史

    Organ Biology　25　(3)　67-67　2018/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  34. 膵島との共移植が肝細胞生着へ及ぼす影響に関する検討

    齋藤 純健, 稲垣 明子, 猪村 武弘, 猪村 梢, Fathi Ibrahim, 小笠原 弘之, 宮城 重人, 亀井 尚, 海野 倫明, 安波 洋一, 後藤 昌史

    Organ Biology　25　(3)　68-68　2018/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  35. 本邦の膵島移植の今後の展開 東北大学が目指す膵島移植の次の展開

    後藤 昌史, 稲垣 明子, 山形 洋平, 村山 和隆, 渡邉 君子, 前田 浩, 猪村 武弘, 五十嵐 康宏, 植松 智海, 宮崎 勇希, 水井 崇浩, Fathi Ibrahim, 戸子台 和哲, 宮城 重人, 藤盛 啓成, 亀井 尚, 海野 倫明

    移植　53　(総会臨時)　275-275　2018/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  36. 膵島から放出される細胞生着促進因子を用いた肝細胞グラフトの成績向上について

    齋藤 純健, 猪村 武弘, 稲垣 明子, 後藤 めぐみ, Fathi Ibrahim, 小笠原 弘之, 宮城 重人, 亀井 尚, 海野 倫明, 安波 洋一, 後藤 昌史

    移植　53　(総会臨時)　514-514　2018/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  37. ドナー膵組織の細胞外マトリックス組成に対応する膵島分離酵素の至適化に向けた試み

    宮崎 勇希, 猪村 武弘, 村山 和隆, Fathi Ibrahim, 山形 洋平, 前田 浩, 渡邉 君子, 稲垣 明子, 宮城 重人, 島 弘季, 五十嵐 和彦, 海野 倫明, 亀井 尚, 後藤 昌史

    移植　53　(総会臨時)　523-523　2018/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  38. 米国から搬送されたヒト膵島の性能に関する検討

    猪村 武弘, 稲垣 明子, Ibrahim Fathi, 後藤 昌史

    移植　53　(総会臨時)　524-524　2018/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  39. 肝細胞と膵島の共移植による肝細胞生着の定量的及び組織学的検討

    齋藤 純健, 猪村 武弘, 稲垣 明子, Fathi Ibrahim, 小笠原 弘之, 宮城 重人, 亀井 尚, 海野 倫明, 安波 洋一, 後藤 昌史

    日本組織移植学会雑誌　17　(1)　48-48　2018/07

    Publisher: (一社)日本組織移植学会

    ISSN： 1347-6491

  40. 肝細胞移植における経門脈移植法と脾実質移植法の比較検討

    小笠原 弘之, 稲垣 明子, 猪村 武弘, 齋藤 純健, 松村 宗幸, 福岡 健吾, Fathi Ibrahim, 宮城 重人, 大橋 一夫, 海野 倫明, 亀井 尚, 後藤 昌史

    日本組織移植学会雑誌　17　(1)　49-49　2018/07

    Publisher: (一社)日本組織移植学会

    ISSN： 1347-6491

  41. The Optimization of the Hepatocyte Surface Modification Procedures in Terms of Heparin and Apyrase for Improving Hepatocyte Engraftment

    Hiroyuki Ogasawara, Yuji Teramura, Takehiro Imura, Akiko Inagaki, Yoshikatsu Saito, Muneyuki Matsumura, Kengo Fukuoka, Ibrahim Fathi, Shigehito Miyagi, Kristina Nilsson, Kazuo Ohashi, Michiaki Unno, Takashi Kamei, Susumu Satomi, Bo Nilsson, Elisabet Gustafson, Masafumi Goto

    TRANSPLANTATION　102　S727-S727　2018/07

    DOI： 10.1097/01.tp.0000543706.60801.e9 　

    ISSN： 0041-1337

    eISSN： 1534-6080

  42. 移植前肝細胞に対するADP/ATP ratioを用いた新規評価法の確立

    松村 宗幸, 猪村 武弘, 小笠原 弘之, 福岡 健吾, 稲垣 明子, 宮城 重人, 大橋 一夫, 海野 倫明, 亀井 尚, 里見 進, 後藤 昌史

    移植　52　(6)　557-557　2018/02

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  43. RGD基を含有する新規コラーゲン素材のリコンビナントペプチド(RCP)を用いた皮下膵島移植の試み

    植松 智海, 稲垣 明子, 中村 保宏, 後藤 めぐみ, 猪村 武弘, 猪村 梢, 五十嵐 康宏, 亀井 尚, 海野 倫明, 里見 進, 後藤 昌史

    移植　52　(6)　565-565　2018/02

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  44. 移植前肝細胞に対するADP/ATP ratioを用いた簡便で有用な評価法の確立

    松村宗幸, 猪村武弘, 小笠原弘之, 福岡健吾, 稲垣明子, 宮城重人, 大橋一夫, 海野倫明, 亀井尚, 里見進, 後藤昌史, 後藤昌史

    日本再生医療学会総会(Web)　17th　2018

  45. 肝細胞移植の生着率向上をめざしたヘパリン及びアピラーゼ肝細胞表面コーティング法の最適化についての検討

    小笠原弘之, 寺村裕治, 寺村裕治, 稲垣明子, 齋藤純健, 松村宗幸, FATHI Ibrahim, 宮城重人, NILSSON Kristina, 大橋一夫, 海野倫明, 亀井尚, NILSSON Bo, GUSTAFSON Elisabet, 後藤昌史, 後藤昌史

    日本移植学会総会プログラム抄録集　54th　(総会臨時)　515-515　2018

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  46. 出血が少なく使いやすい皮下デバイスを目指して:糖尿病マウスにおけるRGD基含有ヒトI型コラーゲン素材による皮下膵島移植についての報告

    植松智海, 稲垣明子, 中村保宏, 後藤めぐみ, 猪村武弘, 猪村梢, 五十嵐康宏, FATHI Ibrahim, 宮城重人, 亀井尚, 内藤剛, 海野倫明, 里見進, 後藤昌史, 後藤昌史

    日本膵・膵島移植研究会プログラム・抄録集　45th　2018

  47. ドナー膵組織のマトリックス組成に対応するテーラーメイド型膵島分離法の確立へ向けた試み

    宮崎勇希, 猪村武弘, 村山和隆, 山形洋平, 前田浩, 渡邉君子, 稲垣明子, 宮城重人, 内藤剛, 海野倫明, 亀井尚, 里見進, 後藤昌史, 後藤昌史

    日本膵・膵島移植研究会プログラム・抄録集　45th　2018

  48. 膵島を用いた細胞生着促進因子を同定する有用な解析モデルの構築

    齋藤純健, 猪村武弘, 後藤めぐみ, 稲垣明子, FATHI Ibrahim, 小笠原弘之, 宮城重人, 亀井尚, 海野倫明, 里見進, 安波洋一, 後藤昌史, 後藤昌史

    日本膵・膵島移植研究会プログラム・抄録集　45th　2018

  49. 細胞移植 RGD基(Arg-Gly-Asp)含有コラーゲン素材を使用した皮下膵島移植における血管新生と移植効率についての報告

    植松 智海, 稲垣 明子, 中村 保宏, 後藤 めぐみ, 猪村 武弘, 猪村 梢, 五十嵐 康宏, 亀井 尚, 海野 倫明, 里見 進, 後藤 昌史

    Organ Biology　24　(3)　78-78　2017/11

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  50. 移植前肝細胞におけるviability評価法としてのADP/ATP assayの有用性に関する検証

    松村 宗幸, 猪村 武弘, 小笠原 弘之, 福岡 健吾, 稲垣 明子, 宮城 重人, 大橋 一夫, 海野 倫明, 亀井 尚, 里見 進, 後藤 昌史

    Organ Biology　24　(3)　91-91　2017/11

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  51. 膵島移植:今後の方向性はいかに? 免疫抑制剤不要の膵島移植実現へ向けて

    後藤 昌史, 稲垣 明子, 猪村 武弘, 五十嵐 康宏, Fathi Ibrahim, 戸子台 和哲, 宮城 重人, 里見 進

    移植　52　(総会臨時)　306-306　2017/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  52. 血管誘導効果を有するRGD基(Arg-Gly-Asp)含有コラーゲン素材を使用した皮下膵島移植法の樹立

    植松 智海, 稲垣 明子, 中村 保宏, 後藤 めぐみ, 猪村 武弘, 五十嵐 康宏, 亀井 尚, 海野 倫明, 里見 進, 後藤 昌史

    移植　52　(総会臨時)　404-404　2017/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  53. 膵島移植:今後の方向性はいかに? 免疫抑制剤不要の膵島移植実現へ向けて

    後藤 昌史, 稲垣 明子, 猪村 武弘, 五十嵐 康宏, Fathi Ibrahim, 戸子台 和哲, 宮城 重人, 里見 進

    移植　52　(総会臨時)　306-306　2017/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

  54. 血管誘導効果を有するRGD基(Arg-Gly-Asp)含有コラーゲン素材を使用した皮下膵島移植法の樹立

    植松 智海, 稲垣 明子, 中村 保宏, 後藤 めぐみ, 猪村 武弘, 五十嵐 康宏, 亀井 尚, 海野 倫明, 里見 進, 後藤 昌史

    移植　52　(総会臨時)　404-404　2017/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

  55. Thioredoxin-1による移植後早期膵島障害の抑制に関する研究

    阿佐美健吾, 稲垣明子, 猪村武弘, 関口悟, 藤盛啓成, 増谷弘, 淀井淳司, 淀井淳司, 里見進, 大内憲明, 後藤昌史, 後藤昌史

    移植(Web)　52　(1)　2017

    ISSN： 2188-0034

  56. RGD基を含有する新規コラーゲン素材のリコンビナントペプチド(RCP)を用いた皮下膵島移植の試み

    植松智海, 稲垣明子, 中村保宏, 後藤めぐみ, 猪村武弘, 猪村梢, 五十嵐康宏, 亀井尚, 海野倫明, 里見進, 後藤昌史, 後藤昌史

    移植(Web)　52　(6)　2017

    ISSN： 2188-0034

  57. 移植前肝細胞に対するADP/ATP ratioを用いた新規評価法の確立

    松村宗幸, 猪村武弘, 小笠原弘之, 福岡健吾, 稲垣明子, 宮城重人, 大橋一夫, 海野倫明, 亀井尚, 里見進, 後藤昌史, 後藤昌史

    移植(Web)　52　(6)　2017

    ISSN： 2188-0034

  58. 血管誘導効果を有するRGD基(Arg-Gly-Asp)含有コラーゲン素材の導入による皮下膵島移植の生着促進の試み

    植松智海, 稲垣明子, 中村保宏, 後藤めぐみ, 猪村武弘, 五十嵐康宏, 大内憲明, 里見進, 後藤昌史, 後藤昌史

    日本膵・膵島移植研究会プログラム・抄録集　44th　2017

  59. 肝細胞移植におけるグラフト保存法の至適化

    福岡 健吾, 松村 宗幸, 吉田 論, 猪村 武弘, 稲垣 明子, 五十嵐 康弘, 宮城 重人, 大橋 一夫, 絵野沢 伸, 大内 憲明, 里見 進, 後藤 昌史

    Organ Biology　23　(3)　79-79　2016/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  60. 肝細胞移植前の分離肝細胞評価法としてのADP/ATP assayの有用性に関する検証

    松村 宗幸, 猪村 武弘, 福岡 健吾, 稲垣 明子, 宮城 重人, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    Organ Biology　23　(3)　80-80　2016/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  61. 膵消化酵素が膵島分離工程で膵島に与える影響

    宮崎 勇希, 猪村 武弘, 山形 洋平, 前田 浩, 村山 和隆, 渡邉 君子, 稲垣 明子, 五十嵐 康宏, 大内 憲明, 里見 進, 後藤 昌史

    Organ Biology　23　(3)　82-82　2016/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  62. 肝細胞移植におけるグラフト保存法の至適化

    福岡 健吾, 松村 宗幸, 吉田 論, 猪村 武弘, 稲垣 明子, 五十嵐 康弘, 宮城 重人, 大橋 一夫, 絵野沢 伸, 大内 憲明, 里見 進, 後藤 昌史

    移植　51　(総会臨時)　256-256　2016/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  63. 実用化へ向けた膵島分離用新規中性プロテアーゼカクテルの構築

    後藤 昌史, 山形 洋平, 村山 和隆, 渡邉 君子, 前田 浩, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 里見 進

    移植　51　(総会臨時)　322-322　2016/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  64. 膵島分離における消化酵素剤の膵島障害性に関する検討

    宮崎 勇希, 猪村 武弘, 山形 洋平, 前田 浩, 村山 和隆, 渡邉 君子, 稲垣 明子, 五十嵐 康宏, 大内 憲明, 里見 進, 後藤 昌史

    移植　51　(総会臨時)　322-322　2016/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  65. 膵島皮下移植を実現するための移植前血管床構築法の至適化

    植松 智海, 稲垣 明子, 後藤 めぐみ, 猪村 武弘, 五十嵐 康宏, 大内 憲明, 里見 進, 後藤 昌史

    移植　51　(総会臨時)　323-323　2016/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  66. 移植前肝細胞評価法としてのADP/ATP assayの有用性に関する検証

    松村 宗幸, 猪村 武弘, 福岡 健吾, 稲垣 明子, 宮城 重人, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    移植　51　(総会臨時)　374-374　2016/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  67. 膵島移植の長期生着のための課題と克服 膵島分離成否の鍵を握る中性プロテアーゼ使用法に関する戦略

    後藤 昌史, 山形 洋平, 村山 和隆, 渡邉 君子, 前田 浩, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 佐藤 真実, 宮崎 勇希, 植松 智海, 宮城 重人, 藤盛 啓成, 大内 憲明, 里見 進

    移植　51　(2-3)　274-274　2016/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  68. 高濃度コラゲナーゼが膵島分離へ及ぼす影響に関する検討

    猪村 武弘, 山形 洋平, 村山 和隆, 渡邉 君子, 稲垣 明子, 五十嵐 康宏, 里見 進, 後藤 昌史

    移植　51　(2-3)　288-288　2016/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  69. テーラーメイド膵島分離実現へ向けたコラゲナーゼサブタイプGの対象基質の検証

    吉田 諭, 山形 洋平, 中川 香奈子, 前田 憲寿, 村山 和隆, 渡邊 君子, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 宮城 重人, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    移植　51　(2-3)　288-288　2016/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  70. 膵島分離工程において消化酵素剤が分離膵島へ及ぼす影響

    宮崎 勇希, 猪村 武弘, 山形 洋平, 前田 浩, 村山 和隆, 渡邉 君子, 稲垣 明子, 五十嵐 康宏, 大内 憲明, 里見 進, 後藤 昌史

    移植　51　(2-3)　289-289　2016/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  71. 膵島皮下移植モデルにおけるCTを用いた移植前血管床評価の試み

    植松 智海, 稲垣 明子, 猪村 梢, 五十嵐 康宏, 大内 憲明, 里見 進, 後藤 昌史

    移植　51　(2-3)　292-292　2016/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  72. 塩基性線維芽細胞増殖因子デバイスによる皮下血管床構築のための霊長類における検証

    稲垣 明子, 猪村 梢, 柏山 浩, 猪村 武弘, 五十嵐 康宏, 末田 輝子, 笠井 憲雪, 小野 文子, 後藤 昌史

    移植　51　(2-3)　292-292　2016/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  73. 【異種移植研究、最近のトピック】国内未発売の臨床グレードStreptozotocinを用いた霊長類糖尿病モデルの作成

    五十嵐 康宏, 猪村 梢, 柏山 浩, 猪村 武弘, 後藤 めぐみ, 稲垣 明子, 末田 輝子, 笠井 憲雪, 小野 文子, 後藤 昌史

    Organ Biology　23　(2)　162-163　2016/07

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  74. The influence of dissociation enzymes on isolated islets during pancreatic islet isolation procedures

    Yuki Miyazaki, Takehiro Imura, Youhei Yamagata, Hiroshi Maeda, Kazutaka Murayama, Kimiko Watanabe, Akiko Inagaki, Yasuhiro Igarashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    TRANSPLANTATION　100　(7)　S59-S59　2016/07

    ISSN： 0041-1337

    eISSN： 1534-6080

  75. The investigation on target matrix of collagenase G for achieving tailor-made islet isolation

    Satoru Yoshida, Youhei Yamagata, Kanako Nakagawa, Kazuhisa Maeda, Kazutaka Murayama, Kimiko Watanabe, Takehiro Imura, Akiko Inagaki, Yasuhiro Igarashi, Shigehito Miyagi, Kazuo Ohashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    TRANSPLANTATION　100　(7)　S849-S849　2016/07

    ISSN： 0041-1337

    eISSN： 1534-6080

  76. 次世代の外科治療における早期探索的医療研究の役割 新規リコンビナント型コラゲナーゼの膵島移植への臨床応用

    後藤 昌史, 山形 洋平, 村山 和隆, 渡邉 君子, 前田 浩, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 宮城 重人, Korsgren Olle, 大内 憲明, 里見 進

    日本外科学会定期学術集会抄録集　116回　WS-3　2016/04

    Publisher: (一社)日本外科学会

  77. 肝細胞移植における移植前グラフト保存法に関する検討

    福岡健吾, 吉田諭, 猪村武弘, 稲垣明子, 五十嵐康宏, 宮城重人, 大橋一夫, 川岸直樹, 大内憲明, 里見進, 後藤昌史, 後藤昌史

    移植(Web)　51　(1)　2016

    ISSN： 2188-0034

  78. PROOF OF CONCEPT FOR THE CLINICAL APPLICATION OF ANIMAL COMPONENT FREE RECOMBINANT COLLAGENASE FOR ISOLATING PANCREATIC ISLETS.

    Masafumi Goto, Andrew Friberg, Magnus Stahle, Takehiro Imura, Youhei Yamagata, Kimiko Watanabe, Kazutaka Murayama, Akiko Inagaki, Yasuhiro Igarashi, Susumu Satomi, Olle Korsgren

    TRANSPLANTATION　99　(11)　S80-S80　2015/11

    ISSN： 0041-1337

    eISSN： 1534-6080

  79. CHARACTERIZATION AND OPTIMIZATION OF ENZYME COMPONENTS FOR HEPATOCYTE ISOLATION

    Satoru Yoshida, Youhei Yamagata, Kanako Nakagawa, Kazutaka Murayama, Kimiko Watanabe, Takehiro Imura, Yasuhiro Igarashi, Akiko Inagaki, Shigehito Miyagi, Kazuo Ohashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    TRANSPLANTATION　99　(11)　S253-S253　2015/11

    ISSN： 0041-1337

    eISSN： 1534-6080

  80. Characterization and optimization of enzyme components for hepatocyte isolation

    Satoru Yoshida, Youhei Yamagata, Kanako Nakagawa, Kazutaka Murayama, Kimiko Watanabe, Takehiro Imura, Yasuhiro Igarashi, Akiko Inagaki, Shigehito Miyagi, Kazuo Ohashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    XENOTRANSPLANTATION　22　S154-S154　2015/11

    ISSN： 0908-665X

    eISSN： 1399-3089

  81. Application of a prominent oxygen-permeable bag for short-time hepatocyte preservation prior to transplantation

    Kengo Fukuoka, Satoru Yoshida, Takehiro Imura, Akiko Inagaki, Yasuhiro Igarashi, Shigehito Miyagi, Kazuo Ohashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    XENOTRANSPLANTATION　22　S44-S45　2015/11

    ISSN： 0908-665X

    eISSN： 1399-3089

  82. THE INFLUENCE OF THE SYNERGISTIC EFFECT OF NEUTRAL PROTEASE DERIVED FROM CLOSTRIDIUM HISTOLYTICUM AND CLOSTRIPAIN ON THE HEPATOCYTE ISOLATION.

    Satoru Yoshida, Youhei Yamagata, Kazutaka Murayama, Kimiko Watanabe, Takehiro Imura, Yasuhiro Igarashi, Akiko Inagaki, Shigehito Miyagi, Kazuo Ohashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    TRANSPLANTATION　99　(11)　S220-S220　2015/11

    ISSN： 0041-1337

    eISSN： 1534-6080

  83. APPLICATION OF A PROMINENT OXYGEN-PERMEABLE BAG FOR SHORT-TIME HEPATOCYTE PRESERVATION PRIOR TO TRANSPLANTATION.

    Kengo Fukuoka, Satoru Yoshida, Takehiro Imura, Akiko Inagaki, Yasuhiro Igarashi, Shigehito Miyagi, Kazuo Ohashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    TRANSPLANTATION　99　(11)　S72-S72　2015/11

    ISSN： 0041-1337

    eISSN： 1534-6080

  84. The influence of the synergistic effect of neutral protease derived from clostridium histolyticum and clostripain on the hepatocyte isolation

    Satoru Yoshida, Youhei Yamagata, Kazutaka Murayama, Kimiko Watanabe, Takehiro Imura, Yasuhiro Igarashi, Akiko Inagaki, Shigehito Miyagi, Kazuo Ohashi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    XENOTRANSPLANTATION　22　S132-S132　2015/11

    ISSN： 0908-665X

    eISSN： 1399-3089

  85. Proof of concept for the clinical application of animal component free recombinant collagenase for isolating pancreatic islets

    Masafumi Goto, Andrew Friberg, Magnus Stahle, Takehiro Imura, Youhei Yamagata, Kimiko Watanabe, Kazutaka Murayama, Akiko Inagaki, Yasuhiro Igarashi, Susumu Satomi, Olle Korsgren

    XENOTRANSPLANTATION　22　S50-S50　2015/11

    ISSN： 0908-665X

    eISSN： 1399-3089

  86. 日本の外科系研究室の異種移植研究(4施設から) 国内未発売臨床グレードStreptozotocin(STZ) Zanosarを用いた霊長類糖尿病モデルの作成

    五十嵐 康宏, 小野 文子, 猪村 梢, 猪村 武弘, 稲垣 明子, 後藤 めぐみ, 佐藤 文俊, 伊藤 貞嘉, 後藤 昌史

    Organ Biology　22　(3)　51-51　2015/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  87. 再生医療新時代の膵・膵島移植の課題と対応 安全で高性能な新規膵島分離用酵素剤のヒト膵臓におけるPOC樹立および今後の戦略

    後藤 昌史, 山形 洋平, 村山 和隆, 渡邉 君子, 前田 浩, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 宮城 重人, 佐藤 真実, 藤盛 啓成, Korsgren Olle, 大内 憲明, 里見 進

    移植　50　(総会臨時)　259-259　2015/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  88. 肝細胞分離における中性プロテアーゼとクロストリパインの併用の有効性に関する検証

    吉田 諭, 山形 洋平, 村山 和隆, 渡邉 君子, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 宮城 重人, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    移植　50　(総会臨時)　392-392　2015/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  89. 肝細胞分離における細胞分離酵素成分の役割解明と至適化

    吉田 諭, 山形 洋平, 中川 香奈子, 村山 和隆, 渡邉 君子, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 宮城 重人, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    移植　50　(総会臨時)　392-392　2015/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  90. 酸素透過性に優れる細胞培養バッグの肝細胞移植への応用

    福岡 健吾, 吉田 諭, 稲垣 明子, 五十嵐 康宏, 宮城 重人, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    移植　50　(総会臨時)　393-393　2015/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  91. 膵島分離酵素剤が膵島へ及ぼす影響 高濃度の酵素剤は膵島障害を引き起こすのか?

    猪村 武弘, 山形 洋平, 村山 和隆, 渡邉 君子, 前田 浩, 稲垣 明子, 五十嵐 康宏, 後藤 昌史

    移植　50　(総会臨時)　443-443　2015/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  92. Secondary Publication 膵島分離におけるコラゲナーゼHの作用機序に関する研究

    藤尾 淳, 村山 和隆, 山形 洋平, 渡邉 君子, 猪村 武弘, 稲垣 明子, 大林 尚美, 島 弘季, 関口 悟, 藤盛 啓成, 五十嵐 和彦, 大内 憲明, 里見 進, 後藤 昌史

    移植　50　(2-3)　216-228　2015/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  93. 肝細胞分離用酵素剤の至適化

    吉田 諭, 山形 洋平, 中川 香奈子, 村山 和隆, 渡邉 君子, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 宮城 重人, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    日本組織移植学会雑誌　14　(1)　40-40　2015/08

    Publisher: 日本組織移植学会

    ISSN： 1347-6491

  94. インスリンデグルデクによる非ヒト霊長類糖尿病モデルの血糖管理の経験

    五十嵐 康宏, 猪村 梢, 柏山 浩, 後藤 めぐみ, 稲垣 明子, 末田 輝子, 笠井 憲雪, 小野 文子, 後藤 昌史

    糖尿病　58　(Suppl.1)　S-364　2015/04

    Publisher: (一社)日本糖尿病学会

    ISSN： 0021-437X

    eISSN： 1881-588X

  95. (Secondary Publication) Collagenase H is crucial for isolation of rat pancreatic islets

    藤尾淳, 村山和隆, 山形洋平, 渡邉君子, 猪村武弘, 稲垣明子, 大林尚美, 大林尚美, 島弘季, 関口悟, 藤盛啓成, 五十嵐和彦, 大内憲明, 里見進, 後藤昌史, 後藤昌史

    移植(Web)　50　(2-3)　2015

    ISSN： 2188-0034

  96. 新規膵島分離用酵素剤の臨床応用と今後の戦略

    後藤昌史, 後藤昌史, 山形洋平, 山形洋平, 村山和隆, 渡邉君子, 前田浩, 前田浩, 猪村武弘, 稲垣明子, 五十嵐康宏, 宮城重人, 戸子台和哲, 田頭真実, 藤盛啓成, KORSGREN Olle, 大内憲明, 里見進

    日本膵・膵島移植研究会プログラム・抄録集　42nd　2015

  97. 肝細胞分離用酵素剤の至適化に関する検討

    吉田 諭, 山形 洋平, 村山 和隆, 渡邉 君子, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    Organ Biology　21　(3)　89-89　2014/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  98. 肝細胞分離におけるコラゲナーゼGの重要性

    吉田 諭, 山形 洋平, 村山 和隆, 渡邉 君子, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 大橋 一夫, 大内 憲明, 里見 進, 後藤 昌史

    日本移植学会総会プログラム抄録集　50回　363-363　2014/08

    Publisher: (一社)日本移植学会

  99. 新規膵島分離用酵素剤の開発

    田頭 真実, 前田 浩, 山形 洋平, 村山 和隆, 渡邉 君子, 猪村 武弘, 稲垣 明子, 五十嵐 康宏, 藤盛 啓成, 大内 憲明, 里見 進, 後藤 昌史

    日本移植学会総会プログラム抄録集　50回　458-458　2014/08

    Publisher: (一社)日本移植学会

  100. 脳死ドナー膵に対する膵臓移植・膵島移植の最適な選択 重度虚血障害により肝臓及び腎臓が使用できないマージナル脳死ドナーからの膵島移植の経験

    後藤 昌史, 五十嵐 康宏, 宮城 重人, 戸子台 和哲, 西村 隆一, 米田 海, 吉田 諭, 佐藤 則子, 伊藤 貴子, 稲垣 明子, 猪村 武弘, 澤田 正二郎, 金子 慶三, 藤盛 啓成, 片桐 秀樹, 大内 憲明, 里見 進

    日本移植学会総会プログラム抄録集　50回　253-253　2014/08

    Publisher: (一社)日本移植学会

  101. 膵臓移植に使用できない脳死ドナー膵臓の膵島移植への有効活用

    後藤 昌史, 五十嵐 康宏, 宮城 重人, 戸子台 和哲, 西村 隆一, 米田 海, 吉田 諭, 佐藤 則子, 伊藤 貴子, 稲垣 明子, 猪村 武弘, 澤田 正二郎, 金子 慶三, 藤盛 啓成, 片桐 秀樹, 大内 憲明, 里見 進

    日本組織移植学会雑誌　13　(1)　130-130　2014/08

    Publisher: 日本組織移植学会

    ISSN： 1347-6491

  102. 膵島移植における細胞性免疫能モニタリングの試み

    五十嵐 康宏, 鈴木 裕之, 相馬 真志, 宮城 重人, 戸子台 和哲, 稲垣 明子, 猪村 武弘, 澤田 正二郎, 金子 慶三, 片桐 秀樹, 藤盛 啓成, 大内 憲明, 里見 進, 島田 美樹, 眞野 成康, 後藤 昌史

    日本移植学会総会プログラム抄録集　50回　461-461　2014/08

    Publisher: (一社)日本移植学会

  103. Synergistic Effect of Neutral Protease and Clostripain On Pancreatic Islet Isolation.

    M. Dendo, Y. Yamagata, K. Murayama, K. Watanabe, H. Maeda, T. Imura, A. Inagaki, Y. Igarashi, K. Fujimori, N. Ohuchi, S. Satomi, M. Goto

    TRANSPLANTATION　98　358-359　2014/07

    ISSN： 0041-1337

    eISSN： 1534-6080

  104. The Influence of Collagen-III Expression on the Efficiency of Cell Isolation by Collagenase H

    Satoru Yoshida, Youhei Yamagata, Kazutaka Murayama, Kimiko Watanabe, Takehiro Imura, Yasuhiro Igarashi, Akiko Inagaki, Keisei Fujimori, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto

    TRANSPLANTATION　96　(6)　S143-S143　2013/09

    ISSN： 0041-1337

    eISSN： 1534-6080

  105. Distinct Role of Collagenase Subtypes in the Isolation of Large Animal Pancreatic Islets

    Masafumi Goto, Youhei Yamagata, Kazutaka Murayama, Kimiko Watanabe, Takehiro Imura, Yasuhiro Igarashi, Akiko Inagaki, Hideyuki Yamaya, Keisei Fujimori, Susumu Satomi

    TRANSPLANTATION　96　(6)　S142-S142　2013/09

    ISSN： 0041-1337

    eISSN： 1534-6080

  106. 肝細胞移植に必要な細胞分離用酵素成分の考察

    吉田諭, 山形洋平, 山形洋平, 村山和隆, 渡邉君子, 猪村武弘, 五十嵐康宏, 稲垣明子, 大橋一夫, 大内憲明, 里見進, 後藤昌史, 後藤昌史

    日本組織移植学会雑誌　12　(1)　49-49　2013

    Publisher: (一社)日本組織移植学会

    ISSN： 1347-6491

  107. 安全で高性能な膵島分離用酵素剤の開発

    後藤昌史, 山形洋平, 村山和隆, 渡邉君子, 前田浩, 稲垣明子, 五十嵐康宏, 藤森啓成, 中川香奈子, 猪村武弘, 里見進

    移植　48　(総会臨時)　292-292　2013

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  108. 細胞分離のCollagenase H至適化において対象組織が発現するコラーゲンIIIの意義

    吉田諭, 山形洋平, 山形洋平, 村山和隆, 渡邉君子, 猪村武弘, 稲垣明子, 五十嵐康宏, 藤盛啓成, 大橋一夫, 大内憲明, 里見進, 後藤昌史, 後藤昌史

    移植　48　(総会臨時)　292-292　2013

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  109. Flt3L-Induced Dendritic Cells Suppress Donor-Specific Immune Reaction but not Prolong the Graft Survival in Pancreatic Islet Transplantation

    A. Fujio, H. Kishimoto, A. Inagaki, S. Sekiguchi, K. Fujimori, S. Satomi, M. Goto

    TRANSPLANTATION　94　(10)　711-711　2012/11

    ISSN： 0041-1337

    eISSN： 1534-6080

  110. 膵島移植における膵管保存法の意義に関する研究

    中西 渉, 稲垣 明子, 猪村 武弘, 中村 保宏, 関口 悟, 藤盛 啓成, 里見 進, 後藤 昌史

    移植　47　(総会臨時)　233-233　2012/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  111. PS-147-8 リコンビナントタイプ新規膵島分離用酵素剤による分離膵島に起因する炎症性メディエーターの制御(PS-147 膵 基礎-1,ポスターセッション,第112回日本外科学会定期学術集会)

    後藤 昌史, 山形 洋平, 渡邉 君子, 村山 和隆, 猪村 武弘, 稲垣 明子, 山谷 英之, 関口 悟, 藤盛 啓成, 黒川 良望, 里見 進

    日本外科学会雑誌　113　(2)　786-786　2012/03/05

    Publisher: 一般社団法人日本外科学会

    ISSN： 0301-4894

  112. インスリンおよび中心静脈栄養を伴う短期絶食が膵島移植後のグラフト生着へ及ぼす影響

    神保琢也, 稲垣明子, 猪村武弘, 関口悟, 中村保宏, 宮川潤一郎, 藤盛啓成, 里見進, 後藤昌史, 後藤昌史

    日本膵・膵島移植研究会プログラム・抄録集　39th　2012

  113. 「膵島移植におけるCell Loss対策」「膵島移植におけるMarginal Donor対策」 膵動静脈奇形に起因する急性腹膜炎に対しグラフト保護プロトコールの導入により良好な結果を得た自家膵島移植症例の報告

    後藤 昌史, 坂田 直昭, 山谷 英之, 元井 冬彦, 山口 賢, 猪村 武弘, 稲垣 明子, 伊藤 経夫, 林 洋毅, 石垣 泰, 片桐 秀樹, 長谷川 豊, 澤田 正二郎, 廣田 衛久, 下瀬川 徹, 関口 悟, 藤盛 啓成, 江川 新一, 海野 倫明, 里見 進

    移植　46　(6)　609-609　2011/12

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  114. 切除した膵島に対する膵臓保存時の虚血ストレスの影響と管理についての研究(A Study on the Influence and Control of Ischemic Stress during Pancreas Preservation on Isolated Islets)

    Zhaoqiang Feng, 後藤 昌史, 猪村 武弘, 稲垣 明子, 山谷 英之, 黒川 良望, 関口 悟, 藤盛 啓成, 淀井 淳司, 里見 進

    移植　46　(6)　615-616　2011/12

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  115. Thioredoxin-1による移植後早期膵島障害の抑制

    阿佐美 健吾, 後藤 昌史, 猪村 武弘, 稲垣 明子, 関口 悟, 藤盛 啓成, 淀井 淳司, 里見 進

    移植　46　(6)　617-618　2011/12

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  116. 次世代の移植医療に向けて 先端技術を応用した移植医療の開発 糖尿病治療のメディカルイノベーション

    後藤 昌史, 山谷 英之, 小川 則彦, 坂田 直昭, 稲垣 明子, 猪村 武弘, 関口 悟, 藤盛 啓成, 黒川 良望, 里見 進

    移植　46　(総会臨時)　123-123　2011/10

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  117. 膵島移植におけるC5a阻害ペプチドの炎症性メディエーターに対する効果についての解析

    中西 渉, 後藤 昌史, 稲垣 明子, 関口 悟, 藤盛 啓成, 岡田 則子, 岡田 秀親, 里見 進

    移植　46　(総会臨時)　203-203　2011/10

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  118. グラフト保護プロトコールの導入により良好な結果を得た急性腹膜炎下における自家膵島移植症例の報告

    後藤 昌史, 坂田 直昭, 山谷 英之, 元井 冬彦, 稲垣 明子, 伊藤 経夫, 石垣 泰, 片桐 秀樹, 長谷川 豊, 廣田 衛久, 下瀬川 徹, 関口 悟, 藤盛 啓成, 江川 新一, 海野 倫明, 里見 進

    移植　46　(総会臨時)　204-204　2011/10

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  119. 臨床応用を目的とした大動物モデルにおける新規膵島評価法の有用性に関する検証

    後藤 昌史, 阿部 宏之, 伊藤 隆広[佐々木], 後藤 めぐみ, 稲垣 明子, 山谷 英之, 関口 悟, 藤盛 啓成, 黒川 良望, 末永 智一, 里見 進

    移植　46　(総会臨時)　205-205　2011/10

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  120. Attenuation of cross-talk between the complement and coagulation cascades by C5a blockade improves early outcomes after intraportal islet transplantation

    TOKODAI Kazuaki, GOTO Masafumi, INAGAKI Akiko, NAKANISHI Wataru, OGAWA Norihiko, SATOH Kazushige, KAWAGISHI Naoki, SEKIGUCHI Satoshi, NILSSON Bo, OKADA Noriko, OKADA Hidechika, SATOMI Susumu

    46　(4)　343-352　2011/09/10

    ISSN： 0578-7947

  121. 膵島移植におけるC5a阻害ペプチドの作用機序に関する検証

    中西 渉, 後藤 昌史, 稲垣 明子, 戸子台 和哲, 岡田 則子, 岡田 秀親, 里見 進

    補体シンポジウム講演集　48　43-43　2011/09

    Publisher: (一社)日本補体学会

    ISSN： 2185-8470

  122. 膵島分離におけるTLA活性の意義に関する検討

    後藤 昌史, 山形 洋平, 渡邉 君子, 村山 和隆, 猪村 武弘, 稲垣 明子, 山谷 英之, 黒川 良望, 里見 進

    Organ Biology　17　(2)　216-216　2010/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  123. 虚血性ストレスが切除したランゲルハンス島に与える影響(Impact of ischemic stress on the quality of isolated islets)

    Zhaoqiang Feng, 後藤 昌史, 猪村 武弘, 稲垣 明子, 山谷 英之, 黒川 良望, 淀井 淳司, 里見 進

    Organ Biology　17　(2)　217-217　2010/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  124. 臨床再開へ向けた膵島分離用酵素剤の比較検討

    後藤 昌史, 山形 洋平, 渡邉 君子, 村山 和隆, 猪村 武弘, 稲垣 明子, 山谷 英之, 小川 則彦, 藤盛 啓成, 黒川 良望, 里見 進

    移植　45　(4)　396-396　2010/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  125. 移植後早期膵島障害の抑制を目的とした新規プロトコールの有効性の評価および作用機序の解明

    戸子台 和哲, 後藤 昌史, 稲垣 明子, 中西 渉, 岡田 則子, 岡田 秀親, 里見 進

    移植　45　(4)　403-403　2010/08

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  126. 長期保存を要する心停止ドナーからの膵島分離への対策

    後藤 昌史, 猪村 武弘, 稲垣 明子, 守屋 俊浩, 中川 栄, 山谷 英之, 藤盛 啓成, 黒川 良望, 里見 進

    移植　45　(3)　297-297　2010/06

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  127. 凝固・補体系の制御による移植後早期膵島障害の抑制

    戸子台 和哲, 後藤 昌史, 稲垣 明子, 岡田 則子, 岡田 秀親, 里見 進

    移植　45　(3)　287-288　2010/06

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  128. 現状における臨床グレードの膵島分離用酵素剤の検証

    後藤昌史, 後藤昌史, 山形洋平, 渡邉君子, 村山和隆, 猪村武弘, 稲垣明子, 山谷英之, 小川則彦, 藤盛啓成, 黒川良望, 里見進

    移植　45　(総会臨時)　227-227　2010

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  129. 安全で高性能な新規膵島分離用酵素剤の開発

    後藤昌史, 後藤昌史, 山形洋平, 渡邉君子, 村山和隆, 猪村武弘, 稲垣明子, 山谷英之, 小川則彦, 藤盛啓成, 黒川良望, 里見進

    移植　45　(総会臨時)　227-227　2010

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  130. 臓器の阻血障害が膵島分離へ及ぼす影響に関する検討

    後藤 昌史, 猪村 武弘, 稲垣 明子, 小川 則彦, 山谷 英之, Feng Qiang, 藤盛 啓成, 黒川 良望, 里見 進

    移植　44　(総会臨時)　280-280　2009/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  131. The impact of ischemic stress on the quality of isolated pancreatic islets

    Masafumi Goto, Takehiro Imura, Akiko Inagaki, Norihiko Ogawa, Hideyuki Yamaya, Keisei Fujimori, Yoshimochi Kurokawa, Susumu Satomi

    XENOTRANSPLANTATION　16　(5)　419-419　2009/09

    ISSN： 0908-665X

  132. A strong candidate approach to prevent the instant blood-mediated inflammatory reaction in clinical islet transplantation

    Kazuaki Tokodai, Masafumi Goto, Akiko Inagaki, Wataru Nakanishi, Noriko Okada, Hidechika Okada, Susumu Satomi

    XENOTRANSPLANTATION　16　(5)　300-300　2009/09

    ISSN： 0908-665X

  133. 臨床膵島移植の成績向上を目的とした凝固・補体系の制御による新規プロトコールの検討

    戸子台 和哲, 後藤 昌史, 稲垣 明子, 岡田 則子, 岡田 秀親, 里見 進

    移植　44　(総会臨時)　277-277　2009/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  134. C5aを標的とした補体阻害ペプチドAcPepA導入による移植後早期膵島障害の抑制

    戸子台 和哲, 後藤 昌史, 稲垣 明子, 中西 渉, 岡田 則子, 岡田 秀親, 里見 進

    補体シンポジウム講演集　46　45-46　2009/08

    Publisher: (一社)日本補体学会

    ISSN： 2185-8470

  135. 有用な膵島評価法である呼吸活性計測法の実際

    後藤 昌史, 阿部 宏之, 伊藤 隆広, 佐々木, 後藤 めぐみ, 稲垣 明子, 小川 則彦, 山谷 英之, 高橋 英幸, 齋藤 之彦, 戸子台 和哲, Feng Qiang, 関口 悟, 藤盛 啓成, 黒川 良望, 里見 進

    移植　43　(総会臨時)　317-317　2008/09

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  136. 高酸素溶液の膵管注入による保存膵組織への影響

    稲垣 明子, 後藤 昌史, 猪村 武弘, 駒林 真理子, 守屋 俊浩, 中川 栄, 小川 則彦, 山谷 英之, 高橋 英幸, 齋藤 之彦, 戸子台 和哲, 関口 悟, 藤盛 啓成, 黒川 良望, 玉井 信, 里見 進

    移植　43　(3)　238-239　2008/06

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  137. 非侵襲性呼吸計測技術の発展的活用による東北大式膵島評価法の確立

    後藤 昌史, 阿部 宏之, 伊藤 隆広, 佐々木, 後藤 めぐみ, 稲垣 明子, 猪村 武弘, 小川 則彦, 山谷 英之, 高橋 英幸, 齋藤 之彦, 戸子台 和哲, 関口 悟, 藤盛 啓成, 黒川 良望, 玉井 信, 里見 進

    移植　43　(3)　240-240　2008/06

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  138. 膵管への高酸素溶液の注入による保存膵組織の酸素化

    稲垣 明子, 後藤 昌史, 猪村 武弘, 駒林 真理子, 守屋 俊浩, 中川 栄, 小川 則彦, 山谷 英之, 高橋 英幸, 齋藤 之彦, 戸子台 和哲, 関口 悟, 藤盛 啓成, 黒川 良望, 玉井 信, 里見 進

    移植　43　(2)　150-150　2008/04

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  139. 糖尿病根治における技術革新 非侵襲呼吸計測技術導入による高精度移植前膵島評価法の確立

    後藤 昌史, 阿部 宏之, 伊藤 隆広[佐々木], 後藤 めぐみ, 稲垣 明子, 藤盛 啓成, 黒川 良望, 玉井 信, 里見 進

    移植　42　(総会臨時)　163-163　2007/10

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  140. 膵管への高酸素溶液の注入による保存膵組織の酸素化

    稲垣 明子, 後藤 昌史, 猪村 武弘, 駒林 真理子, 小川 則彦, 藤盛 啓成, 黒川 良望, 玉井 信, 里見 進

    移植　42　(総会臨時)　308-308　2007/10

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  141. 膵管への高酸素溶液の注入による保存膵組織の酸素化

    稲垣 明子, 後藤 昌史, 猪村 武弘, 駒林 真理子, 守屋 俊浩, 中川 栄, 小川 則彦, 山谷 英之, 高橋 英幸, 齋藤 之彦, 戸子台 和哲, 関口 悟, 藤盛 啓成, 黒川 良望, 玉井 信, 里見 進

    Organ Biology　14　(3)　267-267　2007/10

    Publisher: (一社)日本臓器保存生物医学会

    ISSN： 1340-5152

    eISSN： 2188-0204

  142. DP-101-6 ステントを用いた血管吻合法に関する実験的研究(第107回日本外科学会定期学術集会)

    中野 善之, 堀 義生, 佐藤 成, 渡辺 徹雄, 高田 秀司, 後藤 均, 稲垣 明子, 筏 義人, 里見 進

    日本外科学会雑誌　108　(2)　527-527　2007/03/10

    Publisher: 一般社団法人日本外科学会

    ISSN： 0301-4894

  143. ラット結腸粘膜上皮細胞の増殖に対するコハク酸の抑制効果

    A INAGAKI, H ICHIKAWA, T SAKATA

    Journal of Nutritional Science and Vitaminology　53　(4)　377-379　2007

    DOI： 10.3177/jnsv.53.377 　

  144. Influences og lactic acid ,succinic acid and ammoniaon epithelial cell proliferation and motility of large bowel (Asia Pac J Clin Nutr)

    Sakata T, Ichikawa H, Inagaki A

    Asia Pac J Clin Nutr　8　(Suppl)　9-13　1999/04

Show all ︎Show first 5

Books and Other Publications 2

  1. 消化管の栄養・生理と腸内細菌

    アニマルメディア社（東京）　2010/01

  2. Advanced dietary fibre technology

    Akiko Inagaki, Takashi Sakata

    Blackwell Science　2001/12

    ISBN: 0632056347

Research Projects 9

  1. 移植医療と再生医療を効果的に融合するための革新的基盤技術の構築

    後藤 昌史, 稲垣 明子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04/01 - 2026/03/31

  2. The improvement in islet engraftment using a gelatin hydrogel nonwoven fabric on subcutaneous islet transplantation

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2021/04/01 - 2024/03/31

  3. 従来の常識を覆す革新的免疫抑制剤開発への挑戦

    後藤 昌史, 稲垣 明子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的研究(萌芽)

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2020/07/30 - 2023/03/31

    More details Close

    移植医療の深刻な課題として生涯に渡る免疫抑制剤の服用があげられる。長期に及ぶ免疫抑制剤の服用は患者に様々な副作用をもたらすが、それだけでなくカルシニューリン阻害剤をベースとする現行の世界標準である免疫抑制剤は移植グラフトにも糖尿病や血管障害など多くの悪影響を及ぼすことが明らかとなっている。こうした背景により、カルシニューリン阻害剤に依存しない新規免疫抑制剤の開発が求められている。申請者らはカルシニューリン阻害剤と異なる作用機序を有するKRP-203に着目し、この新規物質が"糖尿病を引き起こさない"、"血管障害を生じない"、"カルシニューリン阻害剤と同等以上に拒絶反応を制御できる"ことを本研究にて実証し、体やグラフトに優しい免疫抑制剤として確立することを目指す。本年度は、昨年度に引き続きKRP-203の新生血管構築阻害に関する検証を目的とし、これまでに我々がその有用性を報告してきたdorsal skinfold chamberから成る高感度イメージングシステムにて、移植膵島グラフト周囲の新生血管体積量の評価を実施した。その結果、薬剤の作用機序から予想した通り、KRP-203は膵島グラフトの新生血管構築阻害を引き起こさないことが明らかとなった。さらに本年度はhigh responderであるB6マウスをレシピエントとした同種同系膵島移植実験を実施し、KRP-203とラパマイシンを組み合わせることにより、同種免疫拒絶反応におけるT細胞およびB細胞のグラフト浸潤を効果的に制御することが可能であることが明らかとなり、本研究の最大の目的であったカルシニューリン阻害剤に依存しない臨床応用可能な免疫制御プロトコールを樹立することができた。本研究における主要目的を達成することができたため、まずはここまでの成果を分野の主要journalであるTransplantationへ報告した。

  4. Development of a basis for establishing the ultimate transplantation medicine that does not require the administration of immunosuppressive drugs

    Goto Masafumi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2018/04/01 - 2022/03/31

    More details Close

    This study was conducted to establish the basis of transplantation medicine that does not require immunosuppressants by combining a new transplantation method to subcutaneous space, which is an ideal transplant site, with an immunoisolated cell device. As a result, it has been clarified that we could cure diabetes for more than 6 months without need of immunosuppressants even in a rat diabetic model, by implanting a gelatin hydrogel nonwoven fabric (GHNF) into the subcutaneous space as pre-treatment, then replacing it with pancreatic islets encapsulated in a novel immunoisolated cell device consisting of modified polyvinyl alcohol. It was also revealed that the dramatic effect of GHNF is due not only to the construction of new blood vessels but also to the compensation of various extracellular matrices and various growth factors derived from host cells (IGF-2, HGF, etc.).

  5. 安全な再生医療実現へ向けた革新的細胞デバイスの構築

    Offer Organization: 日本医療研究開発機構

    System: 再生医療実現拠点ネットワークプログラム （技術開発個別課題）

    Category: 2019年10月 - 2022年3月

    Institution: 東北大学

    2019/10 - 2022/03

  6. The optimization of the prevascularization procedures for improving subcutaneous islet engraftment introducing novel recombinant peptide

    Inagaki Akiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

    More details Close

    Subcutaneous islet transplantation is associated with minimal invasiveness, replicability, and ready removability or re-transplantation thus attracted attention as a new site for islet transplantation. However, subcutaneous tissue involves an issue of lack of vascularization, which is important for engraftment of a transplanted islet graft. Thus, we focused on a recombinant peptide (RCP) device containing a lot of angiogenic RGD and placed the device in a subcutaneous space at a planned site for transplantation, and found that the efficiency of islet engraftment with subcutaneous transplantation was improved to a level comparable to that with intraportal transplantation.

  7. Establishment of next generation cell therapy for diabetes on the prevascularization procedures with Adipose-Derived Stem Cell

    Inagaki Akiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2013/04/01 - 2017/03/31

    More details Close

    Subcutaneous islet transplantation is associated with minimal invasiveness, but poor vascularization. Thus, the optimization of the prevascularization procedures is crucial for improving the outcome. Therefore, in presence study, we evaluated the effectiveness of (Adipose-Derived Stem Cells: ADSC). Although the cure rates in the bFGF group were considerably higher than in the non-device and control group, but there was no significant difference among ADSC group and non-device group on the cure rate.

  8. Development of a Subcutaneous Site Islet Transplantation Method Using Cell Sheet.

    INAGAKI Akiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2011 - 2012

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    We aimed to establish a method to transplant islets of islets to subcutaneous sites with pre-constructed vascular beds by using cell sheets. We found fat-derived stem cells are more appropriate for vascular bed construction prior to subcutaneoustransplantation than basic fibroblast growth factors (bFGF) entailing bloody effusions. In addition, we succeeded in preparing islet cell sheets consisting of islet cell sheets and fibroblasts and in an in vitro sugar tolerance test we confirmed insulin secretion accompanying increase in glucose concentration . However, we could not produce islet cell sheets containing an enough amount of islets to curediabetic animals.

  9. Improvement of pancreatic tissue preservation during organ storage through the introduction of an Islet of Langerhans oxygenation system

    INAGAKI Akiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2009 - 2010

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    Establishing methods to prepare high quality Islets of Langerhans for transplantation is an important subject directly linked to improving transplantation success. This research aims to prevent hypoxic damage to the Islets of Langerhans. We accomplished this by introducing a method to ensure consistent oxygenation from pancreatic organ storage to the completion of pancreatic enzyme treatment. The method involved injecting artificial hemoglobin into the pancreatic ducts to oxygenate the pancreatic tissue during storage. The introduction of oxygenation to the pancreatic ducts via artificial oxygen carriers, as well as the use of hollow fiber pressurization modules within the donor islets clearly improved the output, energy status, and viability of islets and suppressed apoptosis.

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