Details of the Researcher

PHOTO

Yutaka Shibata
Section
Graduate School of Science
Job title
Associate Professor
Degree

  • 博士（理学）（大阪大学）

  • 修士（理学）（大阪大学）

Professional Memberships 6

  • THE JAPANESE SOCIETY OF PHOTOSYNTHESIS RESEARCH

  • 分子科学会

  • 日本植物生理学会

  • 日本化学会

  • 日本生物物理学会

  • 日本物理学会

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Research Areas 3

  • Life sciences / Biophysics /

  • Nanotechnology/Materials / Basic physical chemistry /

  • Natural sciences / Bio-, chemical, and soft-matter physics /

Papers 116

  1. Access to the Antenna System of Photosystem I via Single-Molecule Excitation–Emission Spectroscopy

    Xianjun Zhang, Rin Taniguchi, Ryo Nagao, Tatsuya Tomo, Takumi Noguchi, Shen Ye, Yutaka Shibata

    The Journal of Physical Chemistry B　128　(11)　2664-2674　2024/03/08

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jpcb.3c07789 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  2. Development of Line-Detected UV–Vis Absorption Microscope and Its Application to Quantitative Evaluation of Lithium Surface Reactivity

    Ken-ichi Inoue, Jianxin Mao, Rika Okamoto, Yutaka Shibata, Wenbo Song, Shen Ye

    Analytical Chemistry　2023/02/24

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.analchem.2c05759 　

    ISSN： 0003-2700

    eISSN： 1520-6882

  3. An oxygenic photosynthetic mechanism for using infrared in an Antarctic alga

    Makiko Kosugi, Masato Kawasaki, Yutaka Shibata

    Journal of the Atomic Energy Society of Japan　65　(11)　670-673　2023

    Publisher: Atomic Energy Society of Japan

    DOI： 10.3327/jaesjb.65.11_670 　

    ISSN： 1882-2606

    eISSN： 2433-7285

  4. Accumulation of quenched LHCII around PSI in Chlamydomonas cells in state2 revealed by cryo-fluorescence lifetime imaging

    Yuki Fujita, XianJun Zhang, Ahmed Mohamed, Shen Ye, Yutaka Shibata

    Journal of Photochemistry and Photobiology B: Biology　236　112584-112584　2022/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.jphotobiol.2022.112584 　

    ISSN： 1011-1344

  5. State transition is quiet around pyrenoid and LHCII phosphorylation is not essential for thylakoid deformation in Chlamydomonas 137c

    XianJun Zhang, Yuki Fujita, Naoya Kaneda, Ryutaro Tokutsu, Shen Ye, Jun Minagawa, Yutaka Shibata

    Proceedings of the National Academy of Sciences　119　(37)　2022/09/06

    Publisher: Proceedings of the National Academy of Sciences

    DOI： 10.1073/pnas.2122032119 　

    ISSN： 0027-8424

    eISSN： 1091-6490

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    Photosynthetic organisms have developed a regulation mechanism called state transition (ST) to rapidly adjust the excitation balance between the two photosystems by light-harvesting complex II (LHCII) movement. Though many researchers have assumed coupling of the dynamic transformations of the thylakoid membrane with ST, evidence of that remains elusive. To clarify the above-mentioned coupling in a model organism Chlamydomonas , here we used two advanced microscope techniques, the excitation-spectral microscope (ESM) developed recently by us and the superresolution imaging based on structured-illumination microscopy (SIM). The ESM observation revealed ST-dependent spectral changes upon repeated ST inductions. Surprisingly, it clarified a less significant ST occurrence in the region surrounding the pyrenoid, which is a subcellular compartment specialized for the carbon-fixation reaction, than that in the other domains. Further, we found a species dependence of this phenomenon: 137c strain showed the significant intracellular inhomogeneity of ST occurrence, whereas 4A+ strain hardly did. On the other hand, the SIM observation resolved partially irreversible fine thylakoid transformations caused by the ST-inducing illumination. This fine, irreversible thylakoid transformation was also observed in the STT7 kinase-lacking mutant. This result revealed that the fine thylakoid transformation is not induced solely by the LHCII phosphorylation, suggesting the highly susceptible nature of the thylakoid ultrastructure to the photosynthetic light reactions.

  6. Exciton quenching by oxidized chlorophyll Z across the two adjacent monomers in a photosystem II core dimer

    Ahmed Mohamed, Shunsuke Nishi, Keisuke Kawakami, Jian-Ren Shen, Shigeru Itoh, Hiroshi Fukumura, Yutaka Shibata

    Photosynthesis Research　2022/08/17

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s11120-022-00948-1 　

    ISSN： 0166-8595

    eISSN： 1573-5079

  7. Uphill energy transfer mechanism for photosynthesis in the Antarctic alga

    Makiko Kosugi, Masato Kawasaki, Yutaka Shibata, Kojiro Hara, Shinichi Takaichi, Toshio Moriya, Naruhiko Adachi, Yasuhiro Kamei, Yasuhiro Kashino, Sakae Kudoh, Hiroyuki Koike, Toshiya Senda

    2021/08/05

    Publisher: Research Square Platform LLC

    DOI： 10.21203/rs.3.rs-701485/v1 　

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    Abstract Prasiola crispa, a major green alga in Antarctica, forms layered colonies for survival under the severe terrestrial conditions of Antarctica, which include severe cold, drought, and strong sunlight. As a result of these conditions, the surface cells of P. crispa and other Antarctic organisms face high risk of photodamage. Cells of deeper layer escape from photodamage at the sacrifice of photosynthetic active radiation except infrared. P. crispa achieves effective photosynthesis by low energy far-red light for photosystem II excitation with high efficiency similar to that of visible light. Here, we identified a far-red light-harvesting complex of photosystem II in P. crispa, Pc-frLHC, and proposed a molecular mechanism of uphill excitation energy transfer based on its cryogenic electron-microscopy structure. While Pc-frLHC is associated with photosystem II, it is evolutionarily related to the light-harvesting complex of photosystem I. Pc-frLHC forms a ring-shaped homo-undecamer in which all chlorophyll a molecules are energetically connected and contains chlorophyll a trimers. It seems that the trimers are long-wavelength-absorbing chlorophylls for far-red light at 708 nm, and further absorbance extension is accomplished by Davydov-splitting in dimeric chlorophylls. The chlorophyll network should enable a highly efficient entropy-driven uphill excitation energy transfer using far-red light up to 725 nm.

  8. High-Speed Excitation-Spectral Microscopy Uncovers In Situ Rearrangement of Light-Harvesting Apparatus in Chlamydomonas during State Transitions at Submicron Precision

    Xian Jun Zhang, Yuki Fujita, Ryutaro Tokutsu, Jun Minagawa, Shen Ye, Yutaka Shibata

    Plant and Cell Physiology　2021/04/02

    Publisher: Oxford University Press ({OUP})

    DOI： 10.1093/pcp/pcab047 　

    ISSN： 0032-0781

    eISSN： 1471-9053

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    <title>Abstract</title> Photosynthetic organisms adjust to fluctuating natural light under physiological ambient conditions through flexible light-harvesting ability of light-harvesting complex II (LHCII). A process called state transition is an efficient regulation mechanism to balance the excitations between photosystem II (PSII) and photosystem I (PSI) by shuttling mobile LHCII between them. However, in situ observation of the migration of LHCII in vivo remains limited. In this study, we investigated the in vivo reversible changes in the intracellular distribution of the chlorophyll (Chl) fluorescence during the light-induced state transitions in Chlamydomonas reinhardtii. The newly developed noninvasive excitation-spectral microscope provided powerful spectral information about excitation-energy transfer between Chl-a and Chl-b. The excitation spectra were detected through the fluorescence emission in the 700–750-nm spectral range, where PSII makes the main contribution, though PSI still makes a non-negligible contribution at room temperature. The technique is sensitive to the Chl-b spectral component specifically bound to LHCII. Using a PSI-specific 685-nm component also provided visualization of the local relative concentration of PSI within a chloroplast at room temperature. The decrease in the relative intensity of the Chl-b band in state 2 was more conspicuous in the PSII-rich region than in the PSI-rich region, reflecting the dissociation of LHCII from PSII. We observed intracellular redistributions of the Chl-b-related light-harvesting abilities within a chloroplast during the state transitions. This observation implies the association of the state transitions with the morphological changes in the thylakoid membrane.

  9. Chlamydomonas State Transition Is Quiet Around Pyrenoid and Independent from Thylakoid Deformation Peer-reviewed

    XianJun Zhang, Yuki Fujita, Naoya Kaneda, Ryutaro Tokutsu, Shen Ye, Jun Minagawa, Yutaka Shibata

    bioRxiv　2021/01

    DOI： 10.1101/2021.10.27.465227 　

  10. A gold nanoparticle conjugate with photosystem I and photosystem II for development of a biohybrid water-splitting photocatalyst Peer-reviewed

    Kousuke Kawahara, Natsuko Inoue-Kahino, Keisuke Namie, Yuki Kato, Tatsuya Tomo, Yutaka Shibata, Yasuhiro Kashino, Takumi Noguchi

    Biomedical Spectroscopy and Imaging　9　(1-2)　73-81　2020/05/15

    Publisher: IOS Press

    DOI： 10.3233/bsi-200200 　

    ISSN： 2212-8794

    eISSN： 2212-8808

  11. Development of a Multicolor Line-Focus Microscope for Rapid Acquisitions of Excitation Spectra Peer-reviewed

    Sakar Jana, Yutaka Shibata

    Biophysical Journal　118　36-43　2020/01

    DOI： 10.1016/j.bpj.2019.11.023 　

    ISSN： 0006-3495

  12. Identification of assembly precursors to photosystems emitting fluorescence at 683 nm and 687 nm by cryogenic fluorescence microspectroscopy Peer-reviewed

    Tomofumi Chiba, Yutaka Shibata

    Biochimica et Biophysica Acta (BBA) - Bioenergetics　1860　2019/12

    DOI： 10.1016/j.bbabio.2019.148090 　

    ISSN： 0005-2728

  13. Enhanced Fluorescence Emission and Magnetic Alignment Control of Biphasic Functionalized Composite Janus Particles Peer-reviewed

    Naonari Sakamoto, Yutaro Hirai, Tsunenobu Onodera, Takuma Dezawa, Yutaka Shibata, Hitoshi Kasai, Hidetoshi Oikawa, Hiroshi Yabu

    Particle & Particle Systems Characterization　36　(1)　1800311-1800311　2019/01

    DOI： 10.1002/ppsc.201800311 　

    ISSN： 0934-0866

    eISSN： 1521-4117

  14. Redox-state dependent blinking of single photosystem I trimers at around liquid-nitrogen temperature Peer-reviewed

    Sankar Jana, Ting Du, Ryo Nagao, Takumi Noguchi, Yutaka Shibata

    Biochimica et Biophysica Acta (BBA) - Bioenergetics　1860　30-40　2019/01

    DOI： 10.1016/j.bbabio.2018.11.002 　

    ISSN： 0005-2728

  15. Fabrication of Au-conjugated polymer hybridized nanoparticles and their optical properties Peer-reviewed

    Naonari Sakamoto, Tsunenobu Onodera, Hidetoshi Oikawa, Takuma Dezawa, Yutaka Shibata

    e-J. Surf. Sci., Nanotech.　in press　2018/11

  16. Imaging of intracellular rearrangement of photosynthetic proteins in Chlamydomonas cells upon state transition Peer-reviewed

    Yuki Fujita, Wakana Ito, Kento Washiyama, Yutaka Shibata

    Journal of Photochemistry and Photobiology B: Biology　185　111-116　2018/08

    DOI： 10.1016/j.jphotobiol.2018.05.029 　

    ISSN： 1011-1344

  17. Red shift in the spectrum of a chlorophyll species is essential for the drought-induced dissipation of excess light energy in a poikilohydric moss, Bryum argenteum Peer-reviewed

    Yutaka Shibata, Ahmed Mohamed, Koichiro Taniyama, Kentaro Kanatani, Makiko Kosugi, Hiroshi Fukumura

    PHOTOSYNTHESIS RESEARCH　136　(2)　229-243　2018/05

    DOI： 10.1007/s11120-017-0461-0 　

    ISSN： 0166-8595

    eISSN： 1573-5079

  18. Single bovine serum albumin molecule can hold plural blue-emissive gold nanoclusters: A quantitative study with two-photon excitation Peer-reviewed

    Hamza Al Kindi, Ahmed Mohamed, Shinji Kajimoto, Nurbosyn Zhanpeisov, Hideyuki Horino, Yutaka Shibata, Izabela I. Rzeznicka, Hiroshi Fukumura

    Journal of Photochemistry and Photobiology A: Chemistry　357　168-174　2018/04/15

    DOI： 10.1016/j.jphotochem.2018.02.029 　

    ISSN： 1010-6030

  19. InVivo Energy Transfer from Bacteriochlorophyllc, d, e, or f to Bacteriochlorophylla in Wild-Type and Mutant Cells of the Green Sulfur Bacterium Chlorobaculum limnaeum Peer-reviewed

    Harada Jiro, Shibata Yutaka, Teramura Misato, Mizoguchi Tadashi, Kinoshita Yusuke, Yamamoto Ken, Tamiaki Hitoshi

    CHEMPHOTOCHEM　2　(3)　190-195　2018/03

    DOI： 10.1002/cptc.201700164 　

    ISSN： 2367-0932

  20. Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle Peer-reviewed

    Kazuki Tahara, Ahmed Mohamed, Kousuke Kawahara, Ryo Nagao, Yuki Kato, Hiroshi Fukumura, Yutaka Shibata, Takumi Noguchi

    FARADAY DISCUSSIONS　198　121-134　2017/06

    DOI： 10.1039/c6fd00188b 　

    ISSN： 1359-6640

    eISSN： 1364-5498

  21. Highly Enhanced Emission of Visible Light from Core–Dual-Shell-Type Hybridized Nanoparticles Peer-reviewed

    Sakamoto, N., Onodera, T., Dezawa, T., Shibata, Y., Oikawa, H.

    Particle and Particle Systems Characterization　34　(12)　2017

    DOI： 10.1002/ppsc.201700258 　

    ISSN： 0934-0866

    eISSN： 1521-4117

  22. Structure-Based Modeling of Fluorescence Kinetics of Photosystem II: Relation between Its Dimeric Form and Photoregulation Peer-reviewed

    Mohamed, A., Nagao, R., Noguchi, T., Fukumura, H., Shibata, Y.

    Journal of Physical Chemistry B　120　(3)　365-376　2016

    DOI： 10.1021/acs.jpcb.5b09103 　

    ISSN： 1520-6106

  23. Development of a novel cryogenic microscope with numerical aperture of 0.9 and its application to photosynthesis research Peer-reviewed

    Yutaka Shibata, Wataru Katoh, Tomofumi Chiba, Keisuke Namie, Norikazu Ohnishi, Jun Minagawa, Hanayo Nakanishi, Takumi Noguchi, Hiroshi Fukumura

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS　1837　(6)　880-887　2014/06

    DOI： 10.1016/j.bbabio.2014.03.006 　

    ISSN： 0005-2728

    eISSN： 0006-3002

  24. 3P262 Cryogenic microscope observations of photosynthetic proteins under assembly process in greening etiolated Zea mays leaves(Photobiology: Photosynthesis,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Tomofumi Chiba, Hiroshi Fukumura, Yutaka Shibata

    Seibutsu Butsuri　54　(1)　S292　2014

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.54.S292_4 　

  25. 3P257 Development of an artificial light-driven water splitting nano-device using photosynthetic proteins and metal nanoparticles(Photobiology: Photosynthesis,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Kazuki Tahara, Kousuke Kawahara, Keisuke Namie, Natsuko Inoue, Ryo Nagao, Yuki Kato, Tatsuya Tomo, Yutaka Shibata, Hiroshi Fukumura, Yasuhiro Kashino, Takumi Noguchi

    Seibutsu Butsuri　54　(1)　S291　2014

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.54.S291_5 　

  26. 2P259 Target analysis of the photosystem II-enriched membrane : The effect of oxidizing agent on fluorescence quenching in PSII(18B. Photobiology:Photosynthesis,Poster)

    Mohamed Ahmed, Nagao Ryo, Noguchi Takumi, Fukumura Hiroshi, Shibata Yutaka

    Seibutsu Butsuri　54　(1)　S238　2014

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.54.S238_1 　

  27. Scrambled self-assembly of bacteriochlorophylls c and e in aqueous triton X-100 micelles Peer-reviewed

    Saga, Y., Saiki, T., Takahashi, N., Shibata, Y., Tamiaki, H.

    Photochemistry and Photobiology　90　(3)　552-559　2014

    DOI： 10.1111/php.12219 　

    ISSN： 0031-8655

    eISSN： 1751-1097

  28. Photosystem II Does Not Possess a Simple Excitation Energy Funnel: Time-Resolved Fluorescence Spectroscopy Meets Theory Peer-reviewed

    Yutaka Shibata, Shunsuke Nishi, Keisuke Kawakami, Jian-Ren Shen, Thomas Renger

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　135　(18)　6903-6914　2013/05

    DOI： 10.1021/ja312586p 　

    ISSN： 0002-7863

  29. Study of cell-differentiation and assembly of photosynthetic proteins during greening of etiolated Zea mays leaves using confocal fluorescence microspectroscopy at liquid-nitrogen temperature Peer-reviewed

    Yutaka Shibata, Wataru Katoh, Yukari Tahara

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS　1827　(4)　520-528　2013/04

    DOI： 10.1016/j.bbabio.2013.02.001 　

    ISSN： 0005-2728

    eISSN： 0006-3002

  30. Lichens assist the drought-induced fluorescence quenching of their photobiont green algae through arabitol

    Makiko Kosugi, Akihisa Miyake, Yasuhiro Kasino, Yutaka Shibata, Kazuhiko Satoh, Shigeru Itoh

    Advanced Topics in Science and Technology in China　514-517　2013

    Publisher: Springer

    DOI： 10.1007/978-3-642-32034-7_109 　

    ISSN： 1995-6827 1995-6819

  31. 2P255 Development of a cryogenic optical microscope with NA of 0.9 and its application to studies of pigment distributions in plant cells(18B. Photobiology: Photosynthesis,Poster)

    Shibata Yutaka, Namie Keisuke, Chiba Tomofumi, Kajihara Mizu, Kato Wataru, Fukumura Hiroshi

    Seibutsu Butsuri　53　(1)　S201　2013

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.53.S201_2 　

  32. Two types of fucoxanthin-chlorophyll-binding proteins i tightly bound to the photosystem i core complex in marine centric diatoms Peer-reviewed

    Ikeda, Y., Yamagishi, A., Komura, M., Suzuki, T., Dohmae, N., Shibata, Y., Itoh, S., Koike, H., Satoh, K.

    Biochimica et Biophysica Acta - Bioenergetics　1827　(4)　529-539　2013

    DOI： 10.1016/j.bbabio.2013.02.003 　

    ISSN： 0005-2728

  33. Arabitol provided by lichenous fungi enhances ability to dissipate excess light energy in a symbiotic green alga under desiccation Peer-reviewed

    Kosugi, M., Miyake, H., Yamakawa, H., Shibata, Y., Miyazawa, A., Sugimura, T., Satoh, K., Itoh, S., Kashino, Y.

    Plant and Cell Physiology　54　(8)　1316-1325　2013

    DOI： 10.1093/pcp/pct079 　

    ISSN： 0032-0781 1471-9053

  34. 3D0948 Possible non-photochemical quenching mechanism within pohotosystem II core complex(Photobiology:Photosynthesis,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

    Shibata Yutaka, Nishi Shunsuke, Kawakami Keisuke, Shen Jian-Ren, Renger Thomas

    Seibutsu Butsuri　52　S62　2012

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.52.S62_4 　

  35. Supramolecular energy transfer from photoexcited chlorosomal zinc porphyrin self-aggregates to a chlorin or bacteriochlorin monomer as models of main light-harvesting antenna systems in green photosynthetic bacteria Peer-reviewed

    Kataoka, Y., Shibata, Y., Tamiaki, H.

    Bioorganic and Medicinal Chemistry Letters　22　(16)　5218-5221　2012

    DOI： 10.1016/j.bmcl.2012.06.066 　

    ISSN： 0960-894X

  36. Nanocrystallization of Oligosilanes by a Reprecipitation Method: Easy Fabrication of H-Aggregates of Linear Chain Oligosilane Molecules Peer-reviewed

    Y. Morita, N. Nagaya, Y. Shibata, E. Kwon, H. Oikawa, H. Nakanishi, M. Kira, K. Sakamoto

    Chemical Communications　47　2315-2317　2011/12

    DOI： 10.1039/c0cc03949g 　

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    6957

  37. Multiple dissipation components of excess light energy in dry lichen revealed by ultrafast fluorescence study at 5 K Peer-reviewed

    Miyake, H., Komura, M., Itoh, S., Kosugi, M., Kashino, Y., Satoh, K., Shibata, Y.

    Photosynthesis Research　110　(1)　39-48　2011

    DOI： 10.1007/s11120-011-9691-8 　

    ISSN： 0166-8595

  38. Intensity Borrowing via Excitonic Couplings among Soret and Q(y) Transitions of Bacteriochlorophylls in the Pigment Aggregates of Chlorosomes, the Light-Harvesting Antennae of Green Sulfur Bacteria Peer-reviewed

    Yutaka Shibata, Shingo Tateishi, Shosuke Nakabayashi, Shigeru Itoh, Hitoshi Tamiaki

    BIOCHEMISTRY　49　(35)　7504-7515　2010/09

    DOI： 10.1021/bi100607c 　

    ISSN： 0006-2960

  39. Shallow Sink in an Antenna Pigment System of Photosystem I of a Marine Centric Diatom, Chaetoceros gracilis, Revealed by Ultrafast Fluorescence Spectroscopy at 17 K Peer-reviewed

    Atsushi Yamagishi, Yohei Ikeda, Masayuki Komura, Hiroyuki Koike, Kazuhiko Satoh, Shigeru Itoh, Yutaka Shibata

    JOURNAL OF PHYSICAL CHEMISTRY B　114　(27)　9031-9038　2010/07

    DOI： 10.1021/jp102205v 　

    ISSN： 1520-6106

  40. Kinetically Distinct Three Red Chlorophylls in Photosystem I of Thermosynechococcus elongatus Revealed by Femtosecond Time-Resolved Fluorescence Spectroscopy at 15 K Peer-reviewed

    Yutaka Shibata, Atsushi Yamagishi, Shunsuke Kawamoto, Tomoyasu Noji, Shigeru Itoh

    JOURNAL OF PHYSICAL CHEMISTRY B　114　(8)　2954-2963　2010/03

    DOI： 10.1021/jp909583r 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  41. Spectral properties of single light-harvesting complexes in bacterial photosynthesis Peer-reviewed

    Yoshitaka Saga, Yutaka Shibata, Hitoshi Tamiaki

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS　11　(1)　15-24　2010/03

    DOI： 10.1016/j.jphotochemrev.2010.02.002 　

    ISSN： 1389-5567

  42. Mechanism of strong quenching of photosystem II chlorophyll fluorescence under drought stress in a lichen, Physciella melanchla, studied by subpicosecond fluorescence spectroscopy Peer-reviewed

    Masayuki Komura, Atsushi Yamagishi, Yutaka Shibata, Ikuko Iwasaki, Shigeru Itoh

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS　1797　(3)　331-338　2010/03

    DOI： 10.1016/j.bbabio.2009.11.007 　

    ISSN： 0005-2728

  43. Accumulated Arabitol In Lichen Body Play A Important Role Against Dehydrated Tolerance Of Trebouxia

    Kosugi Makiko, Kashino Yasuhiro, Satoh Kazuhiko, Miyake Hirohisa, Komura Masayuki, Shibata Yutaka, Itoh Shigeru

    Plant and Cell Physiology Supplement　2010　83-83　2010

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2010.0.0083.0 　

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    In order to clarify the role of symbiotic association in desiccation tolerance of photosynthetic partners in lichens, responses to dehydration in a chlorolichen (Ramalina yasudae) and its photobiont (Trebouxia sp.) was studied. Responses to dehydration in the isolated Trebouxia sp. were different from R. yasudae. That is, (1) the photosystem II (PSII) reaction was totally inhibited in R. yasudae when photosynthesis was completely inhibited by desiccation, but it remained active in Trebouxia sp. (2) Dehydration-induced quenching of PSII fluorescence was smaller in the Trebouxia sp. compared to that in R. yasudae. (3) The isolated Trebouxia sp. showed a higher sensitivity to photoinhibition than R. yasudae in the dehydrated condition. We analyzed about the water extract of R. yasudae and found out that arabitol which contained large amount in water extract accelerated quenching of PSII fluorescence of Trebouxia in the dehydrated state. The phenomenon was confirmed by the analysis of picosecond time-resolved fluorescence spectra. In other words, it indicates that accumulated arabitol in lichen body play a important role against dehydrated tolerance of Trebouxia.

  44. Photoprotection by conversion of excess excitation energy into heat in dry lichen and symbiont alga ; Analysis by time-resolved fluorimetry at cryogenic temperature

    Miyake Hirohisa, Tukagoshi Yuta, Komura Masayuki, Yamagishi Atsushi, Shibata Yutaka, Kosugi Makiko, Sato Kazuhiko, Itoh Shigeru

    Plant and Cell Physiology Supplement　2010　770-770　2010

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2010.0.0770.0 　

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    Lichen is a symbiotic organism of a fungi as a host and an alga or (and) a cyanobacterium as a guest. Lichen shows high tolerance to the strong light under drought condition. Recent studies have shown that lichen has a specific mechanism to effectively convert excessive light energy into heat under drought condition. We have performed the time-resolved fluorimetry of lichens at cryogenic temperature to identify the molecular location where this mechanism functions. The study clarified that the quenching of the excitation energy occurs in photosystem II. The lichen containing Trebouxia as a symbiotic alga has a fluorescence component at 740 nm, which seems to contribute to the energy dissipation. In the present study, we report the results in a green alga Trebouxia isolated from a lichen R. yasudae obtained by time-resolved fluorimetry in cryogenic temperature. The isolated Trebouxia showed no rapid energy dissipation upon desiccation as in lichen. However, the ability of rapid energy dissipation was reproduced by drying with a sugar. The results suggest the essential role of the sugar for the drought-tolerance of Trebouxia.

  45. Intramolecular excitation energy transfer from visible-light absorbing chlorophyll derivatives to a near-infrared-light emitting boron dipyrromethene moiety Peer-reviewed

    Kataoka, Y., Shibata, Y., Tamiaki, H.

    Chemistry Letters　39　(9)　953-955　2010

    DOI： 10.1246/cl.2010.953 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  46. Linearly polarized light absorption spectra of chlorosomes, light-harvesting antennas of photosynthetic green sulfur bacteria Peer-reviewed

    Hitoshi Tamiaki, Shingo Tateishi, Shosuke Nakabayashi, Yutaka Shibata, Shigeru Itoh

    CHEMICAL PHYSICS LETTERS　484　(4-6)　333-337　2010/01

    DOI： 10.1016/j.cplett.2009.12.018 　

    ISSN： 0009-2614

    eISSN： 1873-4448

  47. Acceleration of Electron-Transfer-Induced Fluorescence Quenching upon Conversion to the Signaling State in the Blue-Light Receptor, TePixD, from Thermosynechococcus elongatus Peer-reviewed

    Yutaka Shibata, Yoshiya Murai, Yosuke Satoh, Yoshimasa Fukushima, Koji Kajima, Masahiko Ikeuchi, Shigeru Itoh

    JOURNAL OF PHYSICAL CHEMISTRY B　113　(23)　8192-8198　2009/06

    DOI： 10.1021/jp901631b 　

    ISSN： 1520-6106

  48. Anisotropic distribution of emitting transition dipoles in chlorosome from Chlorobium tepidum: fluorescence polarization anisotropy study of single chlorosomes Peer-reviewed

    Yutaka Shibata, Yoshitaka Saga, Hitoshi Tamiaki, Shigeru Itoh

    PHOTOSYNTHESIS RESEARCH　100　(2)　67-78　2009/05

    DOI： 10.1007/s11120-009-9429-z 　

    ISSN： 0166-8595

  49. 2P-223 Comparision of energy-transfer process between photosystem II monomer and dimer from Thermosynechococcus vulucanus studied by time-resolved fluorescence measurement at cryogenic temperature(Photobiology:Photosynthesis,The 47th Annual Meeting of the Biophysical Society of Japan)

    Nishi Shunsuke, Komura Masayuki, Noji Tomoyasu, Kawakami Keisuke, Shen Jian-Ren, Shibata Yutaka, Itoh Shigeru

    Seibutsu Butsuri　49　S142　2009

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.49.S142_2 　

  50. Ultrafast light-harvesting process of Photosystem I reaction center at cryogenic temperature

    Shibata Yutaka, Yamagishi Atsushi, Komura Masayuki, Itoh Shigeru

    Plant and Cell Physiology Supplement　2009　45-45　2009

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2009.0.0045.0 　

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    The light-harvesting and primary charge-separation processes in photosystem I (PS I) reaction center have not been revealed completely, because of 1) the large spectral overlap among many antenna chlorophyll(Chl)s, 2) very fast energy transfers among Chl, and 3) the overlap of the time range between the light harvesting and primary charge separation. At cryogenic temperature, the above difficulties 1) and 2) are expected to be overcome because the Chl spectra become very sharp and the energy transfer becomes drastically slower. We studied the ultrafast fluorescence dynamics of PS I at 15 K. The results showed that at 15 K the antenna Chl in PS I are divided into 3 groups; Chl that transfer the excitation energy to P700 with a 5-ps time constant, 300-ps time constant, and transfer the excitation energy to the red Chl with a 30-ps time constant. Stoichiometry of these Chl groups will be discussed.

  51. Ultra-fast energy transfer process on the isolated antenna-core PSI super complex

    Yamagishi Atsushi, Ikeda Youhei, Koike Hiroyuki, Satoh Kazuhiko, Komura Masayuki, Shibata Yutaka, Itoh Shigeru

    Plant and Cell Physiology Supplement　2009　44-44　2009

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2009.0.0044.0 　

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    Photosystem I(PS I) pigment-protein super complex of diatoms consists of two peripheral antennas, FCP(fucoxanthin-Chlorophyll(Chl)-binding proteins) I-1, FCP I-2, and core complex (PS I-Core). We studied the ultra-fast excitation energy transfer in isolated FCP-PS I super-complex, PS I-Core complex, FCP I-1 and FCP I-2 isolated from C. gracillis by the upconversion and the streak camera methods at 17 K. Results of a global analysis revealed a major fluorescence decay phase with a 5-ps time constant upon the selective excitation of Chl a at 430 nm. However, it was not observed upon the 460-nm excitation that mainly excited Chl c in FCP. Thus, the 5-ps phase was interpreted as the energy trapping in the core. Two components with 0.86-ps and 14-ps time constants were observed upon the excitation at 460 nm suggesting the two energy-transfer pathways from FCP to the core (FCP I-1-> core and FCP I-2-> core).

  52. Drought-induced ultra-fast Fluorescence quenching in Photosystem II in lichens studied by femto-picosecond time-resolved fluorescence spectrophotometry at cryogenic temperature

    Komura Masayuki, Miyake Hiroyuki, Yamagishi Atsushi, Shibata Yutaka, Iwasaki Ikuko, Yamamoto Yoshikazu, Itoh Shigeru

    Plant and Cell Physiology Supplement　2009　578-578　2009

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2009.0.0578.0 　

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    Lichens are photoautotrophic symbionts of fungi and algae and survive even under the drought environments. It has been suggested that dried lichens immediately convert excess light energy into heat to prevent the photoinhibition in photosystem II (PSII). A recent report suggests that the chlorophyll emitting fluorescence at 740 nm (Chl740) is present and acts as an energy quencher in PSII in lichen [1]. We used the same type of lichens and measured ultrafast fluorescence decay in dry lichen cells by the combination of upconversion and streakcamera system at 4-300 K to study whether and where Chl740 is present in PSII.<br>The fluorescence of Chl740 rising within 1 ps after laser excitation, decaying in several picoseconds was observed. The rate of EET and quenching process in PSII was estimated. <br>We also discuss with respect to other two different types of lichens.<br>[1] Veerman et al, Plant Physiol., 145, 997-1005 (2007)

  53. Covalently linked zinc chlorophyll dimers as a model of a chlorophyllous pair in photosynthetic reaction centers Peer-reviewed

    Hitoshi Tamiaki, Kazuhiro Fukai, Hideki Shimazu, Katsunori Nishide, Yutaka Shibata, Shigeru Itoh, Michio Kunieda

    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES　7　(10)　1231-1237　2008/10

    DOI： 10.1039/b802353k 　

    ISSN： 1474-905X

    eISSN： 1474-9092

  54. Temperature-dependent energy gap of the primary charge separation in photosystem I: Study of delayed fluorescence at 77-268 K Peer-reviewed

    Yutaka Shibata, Shinpei Akai, Takashi Kasahara, Isamu Ikegami, Shigeru Itoh

    JOURNAL OF PHYSICAL CHEMISTRY B　112　(21)　6695-6702　2008/05

    DOI： 10.1021/jp710551e 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  55. 3P-270 Sharp zero-phonon lines in fluorescence spectra of single antenna complexes, chlorosomes at cryogenic temperature(The 46th Annual Meeting of the Biophysical Society of Japan)

    Shibata Yutaka, Saga Yoshitaka, Tamiaki Hitoshi, Itoh Shigeru

    Seibutsu Butsuri　48　S169　2008

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.48.S169_2 　

  56. 3P-267 Measurement of diffusion of phycobilisomes inside cyanobacterial cells by fluorescence recovery after photobleaching (FRAP) technique(The 46th Annual Meeting of the Biophysical Society of Japan)

    Yoshikawa Akira, Shibata Yutaka, Itoh Shigeru

    Seibutsu Butsuri　48　S168　2008

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.48.S168_6 　

  57. 3P-268 Light-harvesting process of photosystem I of a diatom Chaetoceros gracillis studied by time-resolved fluorescence at cryogenic temperature(The 46th Annual Meeting of the Biophysical Society of Japan)

    Yamagishi Atsushi, Ikeda Yohei, Koike Hiroyuki, Satoh Kazuhiko, Komura Masayuki, Shibata Yutaka, Itoh Shigeru

    Seibutsu Butsuri　48　S168-S169　2008

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.48.S168_7 　

  58. Ultra-fast up-conversion measurement of chlorophyll fluorescence from dry lichen thalli

    Yamagishi Atsushi, Komura Masayuki, Shibata Yutaka, Ito Shigeru

    Plant and Cell Physiology Supplement　2008　543-543　2008

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2008.0.0543.0 　

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    In the conventional ultra-fast up-conversion fluorescence measurement, fluorescence emitted from the opposite side of the laser excitation is detected. Only a transparent sample could be measured by this type of system. For the noninvasive in vivo measurement, we developed a new reflection-type system, in which the fluorescence emitted at the same side as the laser excitation can be detected. We measured the rise and decay of fluorescence from the green alga inside dried lichen. Lichens are drought tolerant, and have a mechanism to convert the excess light energy into heat to prevent the photoinhibition. We directly measured the time constant of the fluorescence lifetime of dry lichen to be 20 ps. No fluorescence rise corresponding to the dacay was observed suggesting that the most of the excitation energy is quenched within 20 ps.The reflection type system enables us to perform noninvasive ultra-fast measurements of opaque samples like leaves in vivo.

  59. Direct counting of submicrometer-sized photosynthetic apparatus dispersed in medium at cryogenic temperature by confocal laser fluorescence microscopy: Estimation of the number of bacteriochlorophyll c in single light-harvesting antenna complexes chlorosomes of green photosynthetic bacteria Peer-reviewed

    Yoshitaka Saga, Yutaka Shibata, Shigeru Ltoh, Hitoshi Tamiaki

    JOURNAL OF PHYSICAL CHEMISTRY B　111　(43)　12605-12609　2007/11

    DOI： 10.1021/jp071559p 　

    ISSN： 1520-6106

  60. Excited-state dynamics of normal and doubly N-confused type hexaphyrin derivatives studied by time-resolved fluorescence measurements Peer-reviewed

    Jang-Hyun Ryu, Fuyuki Ito, Toshihiko Nagamura, Kiyohisa Nakamura, Hiroyuki Furuta, Yutaka Shibata, Shigeru Itoh

    CHEMICAL PHYSICS LETTERS　443　(4-6)　274-279　2007/08

    DOI： 10.1016/j.cplett.2007.06.097 　

    ISSN： 0009-2614

  61. Polarized fluorescence of aggregated bacteriochlorophyll c and baseplate bacteriochlorophyll a in single chlorosomes isolated from Chloroflexus aurantiacus Peer-reviewed

    Yutaka Shibata, Yoshitaka Saga, Hitoshi Tamiaki, Shigeru Itoh

    BIOCHEMISTRY　46　(23)　7062-7068　2007/06

    DOI： 10.1021/bi0623072 　

    ISSN： 0006-2960

  62. Femtosecond fluorescence spectroscopy and near-field spectroscopy of water-soluble tetra(4-sulfonatophenyl)porphyrin and its J-aggregate (vol 178, pg 192, 2006) Peer-reviewed

    Atsushi Miura, Yutaka Shibata, Haik Chosrowjan, Noboru Mataga, Naoto Tamai

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY　188　(1)　140-140　2007/04

    DOI： 10.1016/j.jphotochem.2006.11.019 　

    ISSN： 1010-6030

  63. Curious temperature depencence of energy gap DG of the photo-induced charge separation in photosynthetic reaction center (in Japanese)

    Shibata, Y, Itoh, S, Ikegami, I

    Photochemistry　38　209-211　2007

  64. Energy and electron transfer in the photosynthetic reaction center complex of Acidiphilium rubrum containing Zn-bacteriochlorophyll a studied by femtosecond up-conversion spectroscopy Peer-reviewed

    Tetsuo Tomi, Yutaka Shibata, Yuki Ikeda, Seiji Taniguchi, Chosrowjan Haik, Noboru Mataga, Keizo Shimada, Shigeru Itoh

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS　1767　(1)　22-30　2007/01

    DOI： 10.1016/j.bbabio.2006.10.008 　

    ISSN： 0005-2728

    eISSN： 1879-2650

  65. A new fluorescence band F689 in photosystem II revealed by picosecond analysis at 4-77 K: function of two terminal energy sinks F689 and F695 in PS II. International-journal

    Masayuki Komura, Yutaka Shibata, Shigeru Itoh

    Biochimica et biophysica acta　1757　(12)　1657-68　2006/12

    ISSN： 0006-3002

    More details Close

    We performed picosecond time-resolved fluorescence spectroscopy in spinach photosystem II (PS II) particles at 4, 40, and 77 K and identified a new fluorescence band, F689. F689 was identified in addition to the well-known F685 and F695 bands in both analyses of decay-associated spectra and global Gaussian deconvolution of time-resolved spectra. Its fast decay suggests the energy transfer directly from F689 to the reaction center chlorophyll P680. The contribution of F689, which increases only at low temperature, explains the unusually broad and variable bandwidth of F695 at low temperature. Global analysis revealed the three types of excitation energy transfer/dissipation processes: (1) energy transfer from the peripheral antenna to the three core antenna bands F685, F689, and F695 with time constants of 29 and 171 ps at 77 and 4 K, respectively; (2) between the three core bands (0.18 and 0.82 ns); and (3) the decays of F689 (0.69 and 3.02 ns) and F695 (2.18 and 4.37 ns). The retardations of these energy transfer rates and the slow F689 decay rate produced the strong blue shift of the PS II fluorescence upon the cooling below 77 K.

  66. A new fluorescence band F689 in photosystem II revealed by picosecond analysis at 4-77 K: Function of two terminal energy sinks F689 and F695 in PSII Peer-reviewed

    Masayuki Komura, Yutaka Shibata, Shigeru Itoh

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS　1757　(12)　1657-1668　2006/12

    DOI： 10.1016/j.bbabio.2006.09.007 　

    ISSN： 0005-2728

  67. Low-temperature fluorescence from single chlorosomes, photosynthetic antenna complexes of green filamentous and sulfur bacteria Peer-reviewed

    Yutaka Shibata, Yoshitaka Saga, Hitoshi Tamiaki, Shigeru Itoh

    BIOPHYSICAL JOURNAL　91　(10)　3787-3796　2006/11

    DOI： 10.1529/biophysj.106.084178 　

    ISSN： 0006-3495

    eISSN： 1542-0086

  68. Fate determination of the flavin photoreceptions in the cyanobacterial blue light receptor TePixD (T110078) Peer-reviewed

    Koji Okajima, Yoshimasa Fukushima, Hiroyuki Suzuki, Akiko Kita, Yuriko Ochiai, Mitsunori Katayama, Yutaka Shibata, Kunio Miki, Takurni Noguchi, Shigeru Itoh, Masahiko Ikeuchi

    JOURNAL OF MOLECULAR BIOLOGY　363　(1)　10-18　2006/10

    DOI： 10.1016/j.jmb.2006.08.005 　

    ISSN： 0022-2836

  69. Feratosecond fluorescence spectroscopy and near-field spectroscopy of water-soluble tetra(4-sulfonatophenyl)porphyrin and its J-aggregate Peer-reviewed

    A Miura, Y Shibata, H Chosrowjan, N Mataga, N Tamai

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY　178　(2-3)　192-200　2006/03

    DOI： 10.1016/j.jphotochem.2005.10.037 　

    ISSN： 1010-6030

  70. 2P346 Femtosecond Fluorescence Up-Conversion Study of Light Harvesting and Electron Transfer in Intact Purple Photosynthetic Bacterial Membrane(43. Photosynthesis,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Shibata Yutaka, Satoh Yosuke, Fujita Daiju, Tomii Tetsuo, Itoh Shigeru

    Seibutsu Butsuri　46　(2)　S382　2006

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.46.S382_2 　

  71. 2P347 Photosynthetic energy and electron transfer in the reaction center complex of Acidiphilium rubrum containing Zn-bacteriochlorophyll a(43. Photosynthesis,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Tomi Tetsuo, Shibata Yutaka, Ikeda Yuki, Taniguchi Seiji, Haik Chosrowjan, Mataga Noboru, Shimada Keizo, Itoh Shigeru

    Seibutsu Butsuri　46　(2)　S382　2006

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.46.S382_3 　

  72. Photoprotection mechanism in dried lichens using pico-second time resolved fluorescence spectrophotometry at cryogenic temperature

    Komura Masayuki, Shibata Yutaka, Iwasaki Ikuko, Itoh Shigeru

    Plant and Cell Physiology Supplement　2006　10-10　2006

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2006.0.010.0 　

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    Lichens are photoautotrophical symbionts containing algae and can live even in the drought environment. It has been suggested that lichens convert absorbed light energy into thermal energy efficiently in the drought conditions, although the mechanism is still unclear. We measured the lifetime of fluorescence of a lichen, Pertusaria sp. using pico-second time resolved spectrophotometer, and analyzed the process of energy conversion. We obtained following results. (1) The fluorescence of photosystem II decayed much faster in the dried cells. (2) Excitation energy transfer within photosystem II was still observed in the dried cells. (3) The lifetime of photosystem II was increased within 1 min after water-absorption. (4) The blue shift and the shortening of the lifetime were also observed in photosystem I upon dehydration. Similar features were observed in other lichens. We discuss this energy dissipation mechanism from these results.

  73. Function of membrane protein in silica nanopores: Incorporation of photosynthetic light-harvesting protein LH2 into FSM Peer-reviewed

    Oda, I, K Hirata, S Watanabe, Y Shibata, T Kajino, Y Fukushima, S Iwai, S Itoh

    JOURNAL OF PHYSICAL CHEMISTRY B　110　(3)　1114-1120　2006/01

    DOI： 10.1021/jp0540860 　

    ISSN： 1520-6106

  74. Excitation energy transfer in individual light-harvesting chlorosome from green photosynthetic bacterium Chloroflexus aurantiacus at cryogenic temperature Peer-reviewed

    Y Saga, H Tamiaki, Y Shibata, S Itoh

    CHEMICAL PHYSICS LETTERS　409　(1-3)　34-37　2005/06

    DOI： 10.1016/j.cplett.2005.04.080 　

    ISSN： 0009-2614

  75. Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria Peer-reviewed

    K Okajima, S Yoshihara, Y Fukushima, XX Geng, M Katayama, S Higashi, M Watanabe, S Sato, S Tabata, Y Shibata, S Itoh, M Ikeuchi

    JOURNAL OF BIOCHEMISTRY　137　(6)　741-750　2005/06

    DOI： 10.1093/jb/mvi089 　

    ISSN： 0021-924X

  76. Primary intermediate in the photocycle of a blue-light sensory blue-light FAD-protein, Tll0078, of Thermosynechococcus elongatus BP-1 Peer-reviewed

    Y Fukushima, K Okajima, Y Shibata, M Ikeuchi, S Itoh

    BIOCHEMISTRY　44　(13)　5149-5158　2005/04

    DOI： 10.1021/bi048044y 　

    ISSN： 0006-2960

  77. 3P284 Function of photosynthetic light-harvesting membrane protein LH2 and Reaction center complex inside silica nano-pores

    Oda I., Ishizaka S., Tsutsui Y., Shibata Y., Dewa M., Kajino T., Fukushima Y., Iwai S., Itoh S.

    Seibutsu Butsuri　45　S274　2005

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.45.S274_4 　

  78. 3P279 Estimation of the number of bacteriochlorophyll-c molecules within a chlorosome by singleapparatus observation at cryogenic temperature

    Shibata Y., Saga Y., Tamiaki H.

    Seibutsu Butsuri　45　S273　2005

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.45.S273_3 　

  79. 2P297 Photoconversion mechanisms of PixD protein studied by site-directed mutagenesis and time-resolved spectroscopy

    Fukushima Y., Okajima K., Shibata Y., Ikeuchi M., Itoh S.

    Seibutsu Butsuri　45　S194　2005

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.45.S194_1 　

  80. Temperature Dependency of Excitation Energy Transfer in Photosystem II of Higher Plants and Cyanobacteria at Cryogenic Temperature

    Komura Masayuki, Shibata Yutaka, Gombos Zoltan, Ajlani Ghada, Itoh Shigeru

    Plant and Cell Physiology Supplement　2005　621-621　2005

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2005.0.621.0 　

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    We investigated excitation energy transfer in Photosystem II (PS II) of higher plants and cyanobacteria at cryogenic temperature. With respect to the steady-state fluorescence spectrum of PS II, the red shift occurs with decreasing temperature to 77 K, however, the opposite blue shift occurs with further decreasing to 5 K. We measured time-resolved fluorescence of spinach PS II at 5 - 77 K and found the new fluorescence band F689. We proposed that the temperature dependency of decay rate of F689 could explain this spectral change.<br> In the present study, we analyzed the cyanobacterial mutant PAL that lacks phycobiliprotein. F689 also existed in the cyanobacterial PS II, however, kinetics of each fluorescence band was different.<br> Kinetic simulations of excitation energy transfer on the basis of these results were performed. We will discuss light-harvesting mechanism in PS II of higher plants and cyanobacteria.

  81. Nanoscale organization of chlorophyll a in mesoporous silica: Efficient energy transfer and stabilized charge separation as in natural photosynthesis Peer-reviewed

    T Itoh, K Yano, T Kajino, S Itoh, Y Shibata, H Mino, R Miyamoto, Y Inada, S Iwai, Y Fukushima

    JOURNAL OF PHYSICAL CHEMISTRY B　108　(36)　13683-13687　2004/09

    DOI： 10.1021/jp047661t 　

    ISSN： 1520-6106

  82. Femtosecond time-resolve fluorescence study of reaction center isolated from a purple bacteria Acidiphilium rubrum containing Zn-bacteriochlorophyll

    T Tomii, Y Shibata, Y Ikeda, S Taniguchi, H Chosrowjan, N Mataga, K Shimada, S Itoh

    PLANT AND CELL PHYSIOLOGY　45　S130-S130　2004

    ISSN： 0032-0781

  83. 1P276 Trapping of a Light-induced Primary Intermediate at 10 K in Photocycle of a blue light sensory BLUF Protein Tl10078

    Fukushima Y., Okajima K., Shibata Y., Ikeuchi M., Itoh S.

    Seibutsu Butsuri　44　S98　2004

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.44.S98_4 　

  84. 2P261 Photosystem II in heterocyst in Nostoc sp. Studied by confocal microspectroscopy

    Sugiura K., Shibata Y., Itoh S.

    Seibutsu Butsuri　44　S175　2004

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.44.S175_1 　

  85. 2P256 Modification of spectral and kinetic properties of fluorescence in phosphatidylglycerol depleted thylakoid membranes

    Komura M., Shibata Y., Domonkos Ildiko, Gombos Zoltan, Wada H., Itoh S.

    Seibutsu Butsuri　44　S173　2004

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.44.S173_4 　

  86. 2P246 Stabilization of photosynthetic antenna protein LH2 by adsorption inside silica nano-pore

    Oda I., Shibata Y., Kajino T., Fukushima Y., Iwai S., Itoh S.

    Seibutsu Butsuri　44　S171　2004

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.44.S171_2 　

  87. Excitation-Energy Transfer in Photosystem II with Fluorescence Decay Measurement at 5 K

    Komura Masayuki, Shibata Yutaka, Itoh Shigeru

    Plant and Cell Physiology Supplement　2004　219-219　2004

    Publisher: The Japanese Society of Plant Physiologists

    DOI： 10.14841/jspp.2004.0.219.0 　

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    It has been reported two fluorescence emission bands at 685 nm(F685) and 695 nm(F695) in photosystem II(PSII) at 77 K, emitted from chlorophyll a in the core antenna CP43 and CP47, respectively. This study, we measured the fluorescence dynamics of a Arabidopsis thaliana leaf in the picosecond time region at 5 K, 40 K and 77 K. We found a new band at 687 nm(F687). With increasing temperature, the decay rate of F687 became faster so that in a later time region it could not be distinguished from F695 because of the overlap with F695. We also observed F687 in the spinach PSII and Synechocystis sp. PCC 6803 at 5-77 K. Each bands in cyanobacteria decay faster than those of higher plants. We will propose a model for the excitation-energy transfer dynamics in PSII, accounts for the rise and decay kinetics of the fluorescence bands.

  88. Simultaneous time resolution of the emission spectra of fluorescent proteins and zooxanthellar chlorophyll in reef-building coral Peer-reviewed

    AM Gilmore, AWD Larkum, A Sallh, S Itoh, Y Shibata, C Bena, H Yamasaki, M Papina, R Van Woesik

    PHOTOCHEMISTRY AND PHOTOBIOLOGY　77　(5)　515-523　2003/05

    DOI： 10.1562/0031-8655(2003)077<0515:STROTE>2.0.CO;2 　

    ISSN： 0031-8655

  89. Absorption and fluorescence spectra of single heterocyst and vegetative cell in Nostoc sp. studied by confocal microscope

    Sugiura K., Shibata Y., Itoh S.

    Seibutsu Butsuri　43　S198　2003

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.43.S198_4 　

  90. Fluorescence spectrum observation of single chlorosome at cryogenic temperature

    Shibata Y., Saga Y., Tamiaki H., Itoh S.

    Seibutsu Butsuri　43　S201　2003

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.43.S201_3 　

  91. Ultrafast charge separation from the S-2 excited state of directly linked porphyrin-imide dyads: First unequivocal observation of the whole bell-shaped energy-gap law and its solvent dependencies Peer-reviewed

    N Mataga, H Chosrowjan, S Taniguchi, Y Shibata, N Yoshida, A Osuka, T Kikuzawa, T Okada

    JOURNAL OF PHYSICAL CHEMISTRY A　106　(51)　12191-12201　2002/12

    DOI： 10.1021/jp021522z 　

    ISSN： 1089-5639

  92. 2D1500 Fluorescence observation of light-harvesting antenna of photosystem II by confocal laser microscope

    Shibata Y., Komura M., Itoh S.

    Seibutsu Butsuri　42　(2)　S109　2002

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.42.S109_1 　

  93. Ultrafast photoinduced reaction dynamics of photoactive yellow protein (PYP): observation of coherent oscillations in the femtosecond fluorescence decay dynamics Peer-reviewed

    N Mataga, H Chosrowjan, Y Shibata, Y Imamoto, M Kataoka, F Tokunaga

    CHEMICAL PHYSICS LETTERS　352　(3-4)　220-225　2002/01

    DOI： 10.1016/S0009-2614(01)01448-8 　

    ISSN： 0009-2614

  94. First unequivocal observation of the whole bell-shaped energy gap law in intramolecular charge separation from S-2 excited state of directly linked porphyrin-imide dyads and its solvent-polarity dependencies Peer-reviewed

    N Mataga, H Chosrowjan, Y Shibata, N Yoshida, A Osuka, T Kikuzawa, T Okada

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　123　(49)　12422-12423　2001/12

    DOI： 10.1021/ja010865s 　

    ISSN： 0002-7863

  95. Excited-state dynamics of rhodopsin probed by femtosecond fluorescence spectroscopy Peer-reviewed

    H Kandori, Y Furutani, S Nishimura, Y Shichida, H Chosrowjan, Y Shibata, N Mataga

    CHEMICAL PHYSICS LETTERS　334　(4-6)　271-276　2001/02

    DOI： 10.1016/S0009-2614(00)01457-3 　

    ISSN： 0009-2614

  96. Time-resolved hole-burning study on myoglobin: Fluctuation of restricted water within distal pocket Peer-reviewed

    Y Shibata, H Ishikawa, S Takahashi, Morishima, I

    BIOPHYSICAL JOURNAL　80　(2)　1013-1023　2001/02

    ISSN： 0006-3495

  97. Femtosecond fluorescence dynamics of porphyrins; internal conversion, energy relaxation, and quantum beats Peer-reviewed

    Y Shibata, H Chosrowjan, N Mataga, N Yoshida, A Osuka

    ULTRAFAST PHENOMENA XII　66　689-691　2001

    ISSN： 0172-6218

  98. Dynamics and mechanisms of ultrafast fluorescence quenching reactions of flavin chromophores in protein nanospace Peer-reviewed

    N Mataga, H Chosrowjan, Y Shibata, F Tanaka, Y Nishina, K Shiga

    JOURNAL OF PHYSICAL CHEMISTRY B　104　(45)　10667-10677　2000/11

    DOI： 10.1021/jp002145y 　

    ISSN： 1089-5647

  99. Effects of modification of protein nanospace structure and change of temperature on the femtosecond to picosecond fluorescence dynamics of photoactive yellow protein Peer-reviewed

    N Mataga, H Chosrowjan, Y Shibata, Y Imamoto, F Tokunaga

    JOURNAL OF PHYSICAL CHEMISTRY B　104　(21)　5191-5199　2000/06

    DOI： 10.1021/jp994205+ 　

    ISSN： 1089-5647

  100. Internal conversion and vibronic relaxation from higher excited electronic state of porphyrins: Femtosecond fluorescence dynamics studies Peer-reviewed

    N Mataga, Y Shibata, H Chosrowjan, N Yoshida, A Osuka

    JOURNAL OF PHYSICAL CHEMISTRY B　104　(17)　4001-4004　2000/05

    DOI： 10.1021/jp9941256 　

    ISSN： 1089-5647

  101. Femtosecond fluorescence studies on ultrafast reaction dynamics of photoactive proteins Peer-reviewed

    N Mataga, H Chosrowjan, Y Shibata, Y Imamoto, F Tokunaga, F Tanaka

    JOURNAL OF LUMINESCENCE　87-9　821-823　2000/05

    DOI： 10.1016/S0022-2313(99)00426-3 　

    ISSN： 0022-2313

  102. Intramolecular energy relaxation and competing electron transfer in porphyrin-acceptor supermolecule systems Peer-reviewed

    Y Shibata, H Chosrowjan, N Mataga, A Osuka

    JOURNAL OF LUMINESCENCE　87-9　757-759　2000/05

    DOI： 10.1016/S0022-2313(99)00388-9 　

    ISSN： 0022-2313

  103. Conformational fluctuation of horseradish Peroxidase observed by time-resolved hole-burning spectroscopy

    Shibata Y.

    Seibutsu Butsuri　40　S8　2000

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.40.S8_3 　

    ISSN： 0582-4052

  104. Conformational fluctuation of native-like and molten-globule-like cytochrome c observed by time-resolved hole burning Peer-reviewed

    Y Shibata, H Takahashi, R Kaneko, A Kurita, T Kushida

    BIOCHEMISTRY　38　(6)　1802-1810　1999/02

    DOI： 10.1021/bi981568b 　

    ISSN： 0006-2960

  105. Solvent effects on conformational dynamics of Zn-substituted myoglobin observed by time-resolved hole-burning spectroscopy Peer-reviewed

    Y Shibata, A Kurita, T Kushida

    BIOCHEMISTRY　38　(6)　1789-1801　1999/02

    DOI： 10.1021/bi9815694 　

    ISSN： 0006-2960

  106. Environmental effects on the femtosecond-picosecond fluorescence dynamics of photoactive yellow protein: Chromophores in aqueous solutions and in protein nanospaces modified by site-directed mutagenesis Peer-reviewed

    H Chosrowjan, N Mataga, Y Shibata, Y Imamoto, F Tokunaga

    JOURNAL OF PHYSICAL CHEMISTRY B　102　(40)　7695-7698　1998/10

    DOI： 10.1021/jp982905t 　

    ISSN： 1089-5647

  107. Rhodopsin emission in real time: A new aspect of the primary event in vision Peer-reviewed

    H Chosrowjan, N Mataga, Y Shibata, S Tachibanaki, H Kandori, Y Shichida, T Okada, T Kouyama

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　120　(37)　9706-9707　1998/09

    DOI： 10.1021/ja981659w 　

    ISSN： 0002-7863

  108. Ultrafast fluorescence quenching dynamics of flavin chromophores in protein nanospace Peer-reviewed

    N Mataga, H Chosrowjan, Y Shibata, F Tanaka

    JOURNAL OF PHYSICAL CHEMISTRY B　102　(37)　7081-7084　1998/09

    DOI： 10.1021/jp982583t 　

    ISSN： 1089-5647

  109. Real-time observation of conformational fluctuations in Zn-substituted myoglobin by time-resolved transient hole-burning spectroscopy Peer-reviewed

    Y Shibata, A Kurita, T Kushida

    BIOPHYSICAL JOURNAL　75　(1)　521-527　1998/07

    DOI： 10.1016/S0006-3495(98)77541-1 　

    ISSN： 0006-3495

  110. Determination of Q(x)- and Q(y)-absorption bands of Zn-porphyrin derivatives contained in proteins by hole-burning spectroscopy Peer-reviewed

    Y Shibata, T Kushida

    CHEMICAL PHYSICS LETTERS　284　(1-2)　115-120　1998/02

    DOI： 10.1016/S0009-2614(97)01366-3 　

    ISSN： 0009-2614

  111. Slow fluctuation in chromophore-solvent systems observed by time-resolved transient hole-burning spectroscopy Peer-reviewed

    A Kurita, K Matsumoto, Y Shibata, T Kushida

    JOURNAL OF LUMINESCENCE　76-7　295-298　1998/02

    DOI： 10.1016/S0022-2313(97)00293-7 　

    ISSN： 0022-2313

  112. Femtosecond-picosecond fluorescence studies on excited state dynamics of photoactive yellow protein (PYP) and it's site-directed mutants Peer-reviewed

    H Chosrowjan, Y Shibata, N Mataga, Y Imamoto, F Tokunaga

    ULTRAFAST PHENOMENA XI　63　684-686　1998

    ISSN： 0172-6218

  113. Temperature and solvent viscosity dependence of conformational dynamics of Zn-substituted myoglobin observed by nanosecond time-resolved transient hole-burning spectroscopy Peer-reviewed

    Y Shibata, H Takahashi, A Kurita, T Kushida

    JOURNAL OF LUMINESCENCE　72-4　605-606　1997/06

    DOI： 10.1016/S0022-2313(96)00394-8 　

    ISSN： 0022-2313

  114. Structural relaxations in H-2-substituted myoglobin observed by temperature-cycling hole burning Peer-reviewed

    Y Shibata, A Kurita, T Kushida

    JOURNAL OF CHEMICAL PHYSICS　104　(12)　4396-4405　1996/03

    DOI： 10.1063/1.471191 　

    ISSN： 0021-9606

  115. Transient hole-burning spectroscopy of Zn-protoporphyrin-substituted myoglobin Peer-reviewed

    Y Shibata, A Kurita, T Kushida

    JOURNAL OF LUMINESCENCE　66-7　(1-6)　13-18　1995/12

    DOI： 10.1016/0022-2313(95)00096-8 　

    ISSN： 0022-2313

  116. 2-LEVEL SYSTEMS IN MYOGLOBIN PROBED BY NON-LORENTZIAN HOLE BROADENING IN A TEMPERATURE-CYCLING EXPERIMENT Peer-reviewed

    A KURITA, Y SHIBATA, T KUSHIDA

    PHYSICAL REVIEW LETTERS　74　(21)　4349-4352　1995/05

    DOI： 10.1103/PhysRevLett.74.4349 　

    ISSN： 0031-9007

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Misc. 20

  1. Protein-protected Au nanoclusters for deep-tissue imaging

    RZEZNICKA Izabela, SHIBATA Yutaka, FUKUMURA Hiroshi

    芝浦工業大学特別教育・研究報告集(CD-ROM)　2016　118‐121　2017/07/31

    ISSN： 2185-7326

  2. 光合成系の光捕集過程を構造に立脚して理解する：理論と実験の融合で見えてきたこと

    柴田 穣

    日本物理学会誌　69　(9)　639-643　2014/09

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 0029-0181

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    The picosecond time-resolved fluorescence spectra of photosystem II (PS II), a major protein of the plant-type photosynthesis, at 5-180K are compared with the simulated spectra based on a microscopic theory. In the theory, exciton relaxations within a group of pigments and exciton transfers between the groups are treated on the basis of the Redfield theory and the generalized Forster theory, respectively. The simulated spectra well reproduced the temperature dependence of the time-resolved fluorescence spectrum of PS II, which is characterized by the emergence of a 695-nm fluorescence peak upon cooling down to 77K and the decrease of its relative intensity upon further cooling below 77K. The study revealed which chlorophyll molecule on the known PS II structure is responsible for the emission at 695nm.

  3. 開口数0.9を持つ極低温顕微鏡の開発

    柴田穣, 加藤渉, 浪江慶祐

    分光研究　63　(3)　103-105　2014

    Publisher: 日本分光学会

    ISSN： 0038-7002

  4. 26aXC-1 Light-harvesting processes of photosynthetic reaction centers of plants : toward its understanding based on the structural information 2

    Shibata Y., Nishi S., Kawakami K., Shen J. R., Renger Thomas

    Meeting abstracts of the Physical Society of Japan　68　(1)　414-414　2013/03/26

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 1342-8349

  5. 光合成系での光捕集過程を構造に立脚して理解する

    柴田 穣

    光合成研究　21　(3)　128-134　2011/12/01

    Publisher: 日本光合成学会

    ISSN： 1884-2852

  6. 27pHC-12 Simulation of time-resolved fluorescence spectrum of photosystem II on the basis of structural information

    Shibata Y., Nishi S., Noji T., Kawakami K., Shen J. R., Renaer Thomas

    Meeting abstracts of the Physical Society of Japan　66　(1)　794-794　2011/03/03

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 1342-8349

  7. 23pED-2 Light-harvesting processes of photosynthetic reaction centers of plants : toward understanding based on the structural information

    Shibata Yutaka, Nishi Shunsuke, Yamagishi Atsushi, Komura Masayuki

    Meeting abstracts of the Physical Society of Japan　65　(1)　409-409　2010/03/01

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 1342-8349

  8. 光合成反応中心における光誘起電荷分離のエネルギーギャップ△Gの奇妙な温度依存性

    柴田 穣, 伊藤 繁, 池上 勇

    2008/12/01

  9. 23pYD-2 Orientation distribution of transition dipoles in photosynthetic antenna apparatus chlorosome made of huge pigment aggregates, studied by fluorescence anisotropy of single molecules at cryogenic temperature

    Shibata Yutaka, Saga Yoshitaka, Tateishi Shingo, Nakabayasi Shosuke, Tamiaki Hitoshi, Itoh Shigeru

    Meeting abstracts of the Physical Society of Japan　63　(1)　357-357　2008/02/29

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 1342-8349

  10. Investigation of Photoprotection Mechanism and Its Diversity in Dry Lichen Studied by Fluorescence Lifetime Analysis

    KOMURA Masayuki, SHIBATA Yutaka, IWASAKI Ikuko, ITOH Shigeru

    119　95-96　2006/12/01

    ISSN： 0918-9440

  11. 15pTA-5 Single molecule detection of chlorosome, a photosynthetic antenna pigment supra-molecule complex, at cryogenic temperature

    Shibata Yutaka, Saga Yoshitaka, Tamiaki Hitoshi, Itoh Shigeru

    Meeting abstracts of the Physical Society of Japan　59　(2)　313-313　2004/08/25

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 1342-8349

  12. Temperature dependence of delayed-fluorescence dynamics of ether-treated PSI reaction center

    Y Shibata, T Kasahara, S Akai, S Itoh, Ikegami, I

    PLANT AND CELL PHYSIOLOGY　45　S43-S43　2004

    ISSN： 0032-0781

  13. 時間分解ホールバーニング分光法による蛋白質構造揺らぎの時間領域観測

    柴田 穣

    生物物理　40　(5)　331-335　2000/09/25

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.40.331 　

    ISSN： 0582-4052

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    Time-resolved transient hole-burning (TRTHB) spectroscopy is quite effective to elucidate the conformational fluctuation dynamics of protein in ns-ms time regime, which has not been investigated intensively so far. It has also great importance for the understanding of the molecular-level mechanism of protein functioning. TRTHB study on Zn-substituted myoglobin has clarified its highly non-exponential fluctuation dynamics. The time scale of the observed fluctuation is distributed over very wide range from ns to μs. The results underscore the utility of the present method to characterize conformational dynamics of protein.

  14. Time-Domain Observation of Conformational Fluctuation of Protein by Time-Resolved Hole-Burning Spectroscopy

    Yutaka Shibata

    Science Progress　83　193-208　2000/08/01

  15. Observation of Structural Fluctuation of Enzyme Protein by Time-Resolved Transient-Hole-Burning Spectroscopy

    Shibata Yutaka

    Meeting abstracts of the Physical Society of Japan　55　(1)　282-282　2000/03/10

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 1342-8349

  16. Femtosecond Spectroscopic Study on Excited-State Dynamics of Water-Soluble Porphyrin J-aggregate.

    三浦篤志, 松村和夫, 玉井尚登, CHOSROWJAN H, 柴田穣, 又賀昇

    光化学討論会講演要旨集　1999　1999

  17. Electron transfer dynamics from higher excited state of Zn-porphyrin-derivative-naphtbylimide super molecular system by femtosecond fluoresecence measurement

    SHIBATA Y., CHOSROWJAN H., MATAGA N., OHSUKA A.

    Meeting abstracts of the Physical Society of Japan　53　(2)　826-826　1998/09/05

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 1342-8349

  18. 6p-YL-8 Slow fluctuation in chromophore-solvent systems observed by time-resolved transient hole-burning spectroscopy

    Kurita A, Matsumoto K, Shibata Y, Kushida T

    Meeting abstracts of the Physical Society of Japan　52　(2)　766-766　1997/09/16

    Publisher: The Physical Society of Japan (JPS)

    ISSN： 1342-8349

  19. 1a-P-9 Observation of the structural fluctuations of Zn-substituted myoglobin by transient hole-burning spectroscopy II

    Shibata Y., Kurita A., Kushida T.

    Abstracts of the meeting of the Physical Society of Japan. Annual meeting　51　(3)　603-603　1996/03/15

    Publisher: The Physical Society of Japan (JPS)

  20. 3p-E-4 Study of the structural fluctuation of Myoglobin by hole burning spectroscopy

    Shibata Y, Kurita A, Kushida T

    Abstracts of the meeting of the Physical Society of Japan. Sectional meeting　1994　(3)　563-563　1994/08/16

    Publisher: The Physical Society of Japan (JPS)

Show all ︎Show first 5

Books and Other Publications 3

  1. Plant Physiology and Development

    Lincoln Taiz, Eduardo Zeiger, Ian Max Moller, Angus, Murphy

    講談社　2017/02

  2. Plant Physiology and Development 6th edition

    SHIBATA Yutaka

    2017

  3. 光合成のエネルギー変換と物質変換

    柴田 穣

    化学同人　2015

Research Projects 19

  1. Elucidation of the uphill excitation energy transfer mechanism and evolutionary process found in an Antarctic alga

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: National Institute for Basic Biology

    2025/04/01 - 2028/03/31

  2. Analysis of Assembly Intermediates of Photosynthetic Pigment Proteins by RNA-Probe-Based Exhaustive Single-Molecule Spectroscopy

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2021/07/09 - 2023/03/31

  3. Search for assembly intermediates of photosynthetic proteins by high-throughput single-molecule spectroscopy

    Shibata Yutaka

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    In order to reveal the mechanism based on which photosynthetic proteins are automatically assembled within biological systems, I aimed to detect the intermediate states of their assembly processes. Since such intermediates are generated only transiently and therefore accumulate only a tiny amount, detection of these components are quite difficult. To overcome the difficulty, I extended the single molecule detection technique to perform an exhaustive survey of all the components within a sample. The present development have established a method to characterize components with a tiny amount within a sample.

  4. 極低温顕微分光による光合成研究の新展開 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    2015/04 - 2018/03

  5. 振電バンド観測による光合成タンパク質の１分子振動分光 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    2015/09 - 2017/09

  6. 励起スペクトル−蛍光スペクトル同時取得可能な走査型光学顕微鏡の開発 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    2014/04 - 2016/03

  7. 新開発の極低温蛍光顕微鏡で観測する光合成タンパク質の動的性質 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    2012/04 - 2015/03

  8. 微気象観測と生理学的実験手法を用いた、南極陸上生物の生理生態の解明 Competitive

    小杉 真貴子

    System: Grant-in-Aid for Scientific Research

    2012/04 - 2014/03

  9. １分子蛍光分光による光合成タンパク質の光捕集機構の解明 Competitive

    柴田 穣

    System: 研究援助金

    2012/04 - 2013/03

  10. 極低温２光子励起顕微鏡による光合成系光エネルギー捕集ダイナミクスの観測 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    2009/04 - 2012/03

  11. 室温から極低温までのピコ・フェムト秒蛍光分光による光合成反応初期過程の研究 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    2005/04 - 2007/03

  12. 新型低エネルギー光合成植物の作成:人為進化への試み Competitive

    伊藤 繁

    System: Grant-in-Aid for Scientific Research

    2005/04 - 2007/03

  13. 新型光合成の機能と構造から反応中心構築原理を探る Competitive

    伊藤 繁

    System: Grant-in-Aid for Scientific Research

    2003/04 - 2005/03

  14. 光合成タンパク質複合体の構造形成過程の追跡 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    2002/04 - 2004/03

  15. 蛋白質ナノスペースにおいて超高速・高効率光反応が特異的に起こるのは何故か Competitive

    又賀 昇

    System: Grant-in-Aid for Scientific Research

    2001/04 - 2003/03

  16. フェムト秒蛍光測定による亜鉛クロロフィルを使う光合成生物の光化学反応の解析 Competitive

    柴田 穣

    System: 研究開発助成金

    2001/04 - 2002/03

  17. 時間分解ホールバーニング分光法を用いた蛋白質構造ダイナミクスの研究 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    1999/04 - 2001/03

  18. 分子内プロトン移動過程における分子振動の選択的励起の効果 Competitive

    柴田 穣

    System: さきがけ研究２１

    1997/10 - 2000/09

  19. 時間分解ホ-ルバ-ニングによる蛋白質の構造ダイナミクスの研究 Competitive

    柴田 穣

    System: Grant-in-Aid for Scientific Research

    1998/04 - 1999/03

Show all Show first 5

Social Activities 1

  1. 化学への招待

    2012/08/03 - 2012/08/04

    More details Close

    以下の３つの実習を行うことで、光と分子が出会った時に起こる現象について、理解を深める。光合成についての基礎も学ぶ。実習１：パセリの葉から光合成色素を抽出し、さらにカラムクロマトグラフィによりクロロフィルa、b、いくつかのカロテノイドに分離する。実習２：実習１で得られた色素溶液などを用いて、光と色の関係や部落ライトによる色素分子からの蛍光についての実験を行う。ルミノールを用いた化学発光についての実験も行う。実習３：野草の切片を作成し、蛍光顕微鏡を用いてその断面を観察する。クロロフィルからの蛍光像を観察することで、葉の内部での葉緑体の分布を知る。また、クロロフィル溶液にベンゾキノンを加えることで、蛍光が消光される実験を行い、電子移動との関係を学ぶ。ＶＭＤというソフトを使って、タンパク質分子の構造をモニター画面上に表示させ、自分で自由に動かすことを体験する。また、protein data bankへアクセスし、参加者が

Other 1

  1. １分子蛍光分光による光合成タンパク質の光捕集機構の解明

    More details Close

    １分子蛍光分光による光合成タンパク質の光捕集機構の解明