TOHOKU UNIVERSITY Researchers

Details of the Researcher

Home

日本語 English

Hitoshi Shiku

Section

Graduate School of Engineering

Job title

Professor

Degree

博士（工学）（東北大学）
修士（工学）（東北大学）

researchmap

https://researchmap.jp/shikuhitoshi

J-GLOBAL ID

200901092327385305

e-Rad No.

10361164

Education 1

Tohoku University　Graduate School of Engineering　Department of Applied Chemistry

1994/04 - 1997/03

Professional Memberships 4

日本分析学会
表面技術協会
日本化学会
The Electrochemical Society of Japan

Research Interests 1

Bioelectrochemistry

Research Areas 4

Nanotechnology/Materials / Optical engineering and photonics /
Life sciences / Biomaterials /
Life sciences / Biomedical engineering /
Nanotechnology/Materials / Basic physical chemistry /

Awards 9

2015年度先端分析技術賞、JAIMA機器開発賞

2015/09/10　（社）日本分析化学会　電気化学計測に基づく受精卵および細胞塊の機能評価装置の開発
電気化学会論文賞

2014/03/30　(社)電気化学会　Electrochemical device with interdigitated ring array electrodes for investigating the relationship between cardiomyocyte differentiation from embryonic stem cells and alkaline phosphatase activity. Electrochemistry 81, 682-687 (2013).
東北分析化学賞

2013/12/16　日本分析化学会東北支部　微小電極探針を用いる受精卵および細胞塊の機能探索に関する研究
Best Paper Award, The Elecgtrochemical Society of Japan

2011/03/25　電気化学会　Electrochemical characterization of enzyme and immunoglobulin G patterned using microcontact printing. Electrochemistry 78, 122-125 (2010).
Well-Received Oral Presentation Award

2010/12/19　The International Congress of Pacific Basin Societies, Recent Advances in Bioanalysis: Ultra-Small Volumes, Global Metabolite Profiling and Single Cells (#56)
市村学術賞（貢献賞）

2009/04/28　新技術開発財団　超高精度・非侵襲細胞呼吸計測装置の開発と医療応用
The Young Scientists' Prize, The Commendation for Science and Technology by the Minister of Education,Culture, Sports, Science and Technology

2009/04/14　文部科学省　微小電極法に基づく初期胚および細胞機能探索の研究
電気化学会進歩賞・佐野賞

2004/03/25　電気化学会　走査型電気化学顕微鏡による生体機能表面の構築と評価に関する研究
山形県科学技術奨励賞

2003/04/15　山形県　走査型電気化学顕微鏡によるウシ体外培養胚の品質評価

Show all ︎Show 5

Papers 319

Fabrication of three-dimensional calcium alginate hydrogels using sacrificial templates of sugar Peer-reviewed

Kosuke Ino, Mika T. Fukuda, Kaoru Hiramoto, Noriko Taira, Yuji Nashimoto, Hitoshi Shiku

Journal of Bioscience and Bioengineering　130　(5)　539-544　2020/11
Publisher: Elsevier BV
DOI： 10.1016/j.jbiosc.2020.06.014 　

ISSN： 1389-1723

More details Close

Hydrogels are receiving increasing attention in bioapplications. Among hydrogels, calcium alginate (Ca-alginate) hydrogels are widely used for their biocompatibility, low toxicity, low cost, and rapid fabrication by simple mixing of Ca2+ and sodium alginate (Na-alginate). For bioapplications using hydrogels, it is necessary to construct designed hydrogel structures. Although several methods have been proposed for fabricating designed hydrogels, a simple and low-cost method is desirable. Therefore, we developed a new method using sacrificial templates of sugar structures to fabricate three-dimensional (3D) designed Ca-alginate hydrogels. In this method, Na-alginate solution is mixed with molten sugar, and the resulting highly viscous material used to mold 3D sugar structures as sacrificial templates. Since sugar constructs are easily handled compared to hydrogels, sugar templates are useful for preparing 3D constructs. Finally, the sugar and Na-alginate structure is immersed in a CaCl2 solution to simultaneously dissolve the template and form the Ca-alginate hydrogel. The resulting hydrogel takes the shape of the sugar template. By stacking and fusing various sugar structures, such as fibers and blocks, 3D designed Ca-alginate hydrogels can be successfully fabricated. This simple and low-cost method shows excellent potential for application to a variety of bioapplications.
Electrochemical measurement of respiratory activity for evaluation of fibroblast spheroids containing endothelial cell networks Peer-reviewed

Kaoru Hiramoto, Hao-Jen Pai, Kosuke Ino, Yuji Nashimoto, Hitoshi Shiku

Electrochimica Acta　340　135979-135979　2020/04
Publisher: Elsevier BV
DOI： 10.1016/j.electacta.2020.135979 　

ISSN： 0013-4686
Closed Bipolar Electrode Array for On-Chip Analysis of Cellular Respiration by Cell Aggregates Peer-reviewed

Kosuke Ino, Ryosuke Yaegaki, Kaoru Hiramoto, Yuji Nashimoto, Hitoshi Shiku

ACS Sensors　5　(3)　740-745　2020/03/27
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acssensors.9b02061 　

ISSN： 2379-3694

eISSN： 2379-3694
Oxygen consumption rate of tumour spheroids during necrotic-like core formation International-journal Peer-reviewed

Rei Mukomoto, Yuji Nashimoto, Takato Terai, Takuto Imaizumi, Kaoru Hiramoto, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Hitoshi Shiku

The Analyst　145　(19)　6342-6348　2020
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d0an00979b 　

ISSN： 0003-2654

eISSN： 1364-5528

More details Close

Scanning electrochemical microscopy (SECM) is a powerful method for evaluating three-dimensional culture models. In this study, we investigated the oxygen consumption rate of tumor spheroids during the formation of necrotic-like core using SECM.
Bioimaging using bipolar electrochemical microscopy with improved spatial resolution Peer-reviewed

Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku

The Analyst　145　(21)　6895-6900　2020
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d0an00912a 　

ISSN： 0003-2654

eISSN： 1364-5528
High Resolution Electrochemical Mapping of the Hydrogen Evolution Reaction on Transition Metal Dichalcogenide Nanosheets. Peer-reviewed

Takahashi Y, Kobayashi Y, Wang Z, Ito Y, Ota M, Ida H, Kumatani A, Miyazawa K, Fujita T, Shiku H, Korchev YE, Miyata Y, Fukuma T, Chen M, Matsue T

Angewandte Chemie (International ed. in English)　59　(9)　3601-3608　2020/01

DOI： 10.1002/anie.201912863 　

ISSN： 1433-7851

eISSN： 1521-3773
Genipin crosslinking of electrodeposited chitosan/gelatin hydrogels for cell culture. Peer-reviewed

A. Tamura, K. Hiramoto, K. Ino, N. Taira, Y. Nashimoto, H. Shiku

Chem. Lett.　48　(10)　1178-1180　2019/08

DOI： 10.1246/cl.190466 　
A Nanopore-based Endotoxin Sensor Peer-reviewed

Kentaro ITO, Kumi Y. INOUE, Takahiro ITO-SASAKI, Tomokazu MATSUE, Hitoshi SHIKU

Bunseki Kagaku　68　(8)　575-580　2019/08

DOI： 10.2116/bunsekikagaku.68.575 　

ISSN： 0525-1931
Site-specific cytosol sampling from a single cell in an intact tumor spheroid using an electrochemical syringe. Peer-reviewed

Y. Nashimoto, M. Echigo, K. Ino, H. Shiku

Analytical Chemistry,　91　(14)　8772-8776　2019/06

DOI： 10.1021/acs.analchem.9b02062 　
Combination of double-mediator system with large-scale integration-based amperometric devices for detecting NAD(P)H:quinone oxidoreductase 1 activity of cancer cell aggregates. Peer-reviewed

K. Ino, T. Onodera, M. T. Fukuda, Y. Nashimoto, H. Shiku

ACS Sensors　4　(6)　1619-1625　2019/06

DOI： 10.1021/acssensors.9b00344 　
A highly sensitive endotoxin sensor based on redox cycling in a nanocavity. Peer-reviewed

K. Ito, K. Y. Inoue, K. Ino, T. Matsue, H. Shiku

Analyst　144　(11)　3659-3667　2019/04
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/C9AN00478E 　

ISSN： 0003-2654

eISSN： 1364-5528

More details Close

A highly sensitive endotoxin sensor and novel analytical principle using diffusion coefficient difference was developed using a nanocavity device.
Electrodeposition-based rapid bioprinting of 3D-designed hydrogels with a pin art device. Peer-reviewed

N. Taira, K. Ino, H. Ida, Y. Nashimoto, H. Shiku

11　035018　2019/04

DOI： 10.1088/1758-5090/ab166e 　
Electrochemical fabrication of fibrin gels bia cascade reaction for cell culture. Peer-reviewed

N. Taira, K. Ino, T. Kumagai, Y. Nashimoto, H. Shiku

Chem Commun.　55　(37)　5335-5338　2019/03

DOI： 10.1039/c9cc01576k 　
Different electrochemicolor imaging using LSI-besed device for simultaneous detection of multiple analytes. Peer-reviewed

K. Ino, T. Onodera, Y. Nashimoto, H. Shiku

Sensors and Materials　31　(1)　13-22　2019/01

DOI： 10.18494/SAM.2019.2035 　
Electrochemical Imaging of Cell Activity in Hydrogels Embedded in Grid-Shaped Polycaprolactone Scaffolds Using a Large-scale Integration (LSI)-based Amperometric Device. Peer-reviewed

K. Ino, Y. Yokokawa, N. Taira, A. Suda, R. Kunikata, Y. Nashimoto, T. Matsue, H. Shiku

Anal. Sci.　35　(1)　39-43　2019/01

DOI： 10.2116/analsci.18SDP01 　
Quantitative Real-Time Monitoring of Antibody-Induced Internalization of Epidermal Growth Factor Receptors on Single Living Mammalian Cells by Scanning Electrochemical Microscopy. Peer-reviewed

Y. Matsumae, Y. Takahashi, H. Shiku, T. Matsue

ChemElectroChem　5　(20)　3096-3101　2018/12

DOI： 10.1002/celc.201800563 　

ISSN： 2196-0216
Hydrogel electrodeposition based on bipolar electrochemistry. Peer-reviewed

K. Ino, T. Matsumoto, N. Taira, T. Kumagai, Y. Nashimoto, H. Shiku

Lab Chip　18　(16)　2425-2432　2018/09

DOI： 10.1039/c8lc00465j 　
Electrochemical printing of calcium alginate/gelatin hydrogel Peer-reviewed

Noriko Taira, Kosuke Ino, Jordan Robert, Hitoshi Shiku

Electrochimica Acta　281　429-436　2018/08/10
Publisher: Elsevier Ltd
DOI： 10.1016/j.electacta.2018.05.124 　

ISSN： 0013-4686
Electrochemicolor imaging of endogenous alkaline phosphatase and respiratory activities of mesenchymal stem cell aggregates in early-stage osteodifferentiation Peer-reviewed

Kosuke Ino, Takehiro Onodera, Yusuke Kanno, Atsushi Suda, Ryota Kunikata, Tomokazu Matsue, Hitoshi Shiku

Electrochimica Acta　268　554-561　2018/04/01
Publisher: Elsevier Ltd
DOI： 10.1016/j.electacta.2018.02.094 　

ISSN： 0013-4686
Erratum to: An approach to the research on ion and water properties in the interphase between the plasma membrane and bulk extracellular solution. Peer-reviewed

Hiroshi Hibino, Madoka Takai, Hidenori Noguchi, Seishiro Sawamura, Yasufumi Takahashi, Hideki Sakai, Hitoshi Shiku

The journal of physiological sciences : JPS　68　(1)　101-101　2018/01

DOI： 10.1007/s12576-017-0572-6 　

More details Close

The article An approach to the research on ion and water properties in the interphase between the plasma membrane and bulk extracellular solution, written by Hiroshi Hibino, Madoka Takai, Hidenori Noguchi, Seishiro Sawamura, Yasufumi Takahashi, Hideki Sakai and Hitoshi Shiku, was originally published Online First without open access.
Electrochemicolor Imaging Using an LSI-Based Device for Multiplexed Cell Assays Peer-reviewed

Yusuke Kanno, Kosuke Ino, Hiroya Abe, Chika Sakamoto, Takehiro Onodera, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　89　(23)　12778-12786　2017/12

DOI： 10.1021/acs.analchem.7b03042 　

ISSN： 0003-2700

eISSN： 1520-6882
3D electrochemical and ion current imaging using scanning electrochemical-scanning ion conductance microscopy Peer-reviewed

Yasufumi Takahashi, Hiroki Ida, Yoshiharu Matsumae, Hirokazu Komaki, Yuanshu Zhou, Akichika Kumatani, Makoto Kanzaki, Hitoshi Shiku, Tomokazu Matsue

PHYSICAL CHEMISTRY CHEMICAL PHYSICS　19　(39)　26728-26733　2017/10

DOI： 10.1039/c7cp05157c 　

ISSN： 1463-9076

eISSN： 1463-9084
Development of Oxygen Consumption Analysis with an on-Chip Electrochemical Device and Simulation Peer-reviewed

Kaoru Hiramoto, Masahiro Yasumi, Hiroshi Ushio, Atsushi Shunori, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　89　(19)　10303-10310　2017/10

DOI： 10.1021/acs.analchem.7b02074 　

ISSN： 0003-2700

eISSN： 1520-6882
Electrochemical Motion Tracking of Microorganisms Using a Large-Scale-Integration-Based Amperometric Device Peer-reviewed

Kosuke Ino, Yusuke Kanno, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　56　(24)　6818-6822　2017/06

DOI： 10.1002/anie.201701541 　

ISSN： 1433-7851

eISSN： 1521-3773
High Speed Scanning Ion Conductance Microscopy for Quantitative Analysis of Nanoscale Dynamics of Microvilli Peer-reviewed

Hiroki Ida, Yasufumi Takahashi, Akichika Kumatani, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　89　(11)　6016-6021　2017/06

DOI： 10.1021/acs.analchem.7b00584 　

ISSN： 0003-2700

eISSN： 1520-6882
Continuous collection and simultaneous detection of picoliter volume of nucleic acid samples using a mille-feuille probe Peer-reviewed

Hidenori Ito, Motoki Tanaka, Yuanshu Zhou, Yuji Nashimoto, Yasufumi Takahashi, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

ANALYTICAL AND BIOANALYTICAL CHEMISTRY　409　(4)　961-969　2017/02

DOI： 10.1007/s00216-016-0006-y 　

ISSN： 1618-2642

eISSN： 1618-2650
Evaluation of mRNA Localization Using Double Barrel Scanning Ion Conductance Microscopy Peer-reviewed

Yuji Nashimoto, Yasufumi Takahashi, Yuanshu Zhou, Hidenori Ito, Hiroki Ida, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

ACS Nano　10　(7)　6915-6922　2016/07

DOI： 10.1021/acsnano.6b02753 　

ISSN： 1936-0851

eISSN： 1936-086X
Sequential Monitoring of Oxygen Consumption Rate of Mouse Embryoid Bodies in Glucose-Depleted Solution Peer-reviewed

Hitoshi Shiku, Nana Aoki, Toshiharu Arai, Yuanshu Zhou, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue

ELECTROCHEMISTRY　84　(5)　302-304　2016/05

DOI： 10.5796/electrochemistry.84.302 　

ISSN： 1344-3542
Hybrid hydrogel-aligned carbon nanotube scaffolds to enhance cardiac differentiation of embryoid bodies Peer-reviewed

Samad Ahadian, Shukuyo Yamada, Javier Ramon-Azcon, Mehdi Estili, Xiaobin Liang, Ken Nakajima, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

ACTA BIOMATERIALIA　31　134-143　2016/02

DOI： 10.1016/j.actbio.2015.11.047 　

ISSN： 1742-7061

eISSN： 1878-7568
Localized Gene Expression Analysis during Sprouting Angiogenesis in Mouse Embryoid Bodies Using a Double Barrel Carbon Probe Peer-reviewed

Hidenori Ito, Yuji Nashimoto, Yuanshu Zhou, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　88　(1)　610-613　2016/01

DOI： 10.1021/acs.analchem.5b04338 　

ISSN： 0003-2700

eISSN： 1520-6882
Evaluation of senescence in individual MCF-7 spheroids based on electrochemical measurement of senescence-associated beta-galactosidase activity Peer-reviewed

Yuanshu Zhou, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHIMICA ACTA　186　449-454　2015/12

DOI： 10.1016/j.electacta.2015.10.115 　

ISSN： 0013-4686

eISSN： 1873-3859
Nanoscale Imaging of an Unlabeled Secretory Protein in Living Cells Using Scanning Ion Conductance Microscopy Peer-reviewed

Yuji Nashimoto, Yasufumi Takahashi, Hiroki Ida, Yoshiharu Matsumae, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　87　(5)　2542-2545　2015/03

DOI： 10.1021/ac5046388 　

ISSN： 0003-2700

eISSN： 1520-6882
Improving the Electrochemical Imaging Sensitivity of Scanning Electrochemical Microscopy-Scanning Ion Conductance Microscopy by Using Electrochemical Pt Deposition Peer-reviewed

Mustafa Sen, Yasufumi Takahashi, Yoshiharu Matsumae, Yoshiko Horiguchi, Akichika Kumatani, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　87　(6)　3484-3489　2015/03

DOI： 10.1021/acs.analchem.5b00027 　

ISSN： 0003-2700

eISSN： 1520-6882
Advanced LSI-based amperometric sensor array with light-shielding structure for effective removal of photocurrent and mode selectable function for individual operation of 400 electrodes Peer-reviewed

Kumi Y. Inoue, Masahki Matsudaira, Masanori Nakano, Kosuke Ino, Chika Sakamoto, Yusuke Kanno, Reyushi Kubo, Ryota Kunikata, Atsushi Kira, Atsushi Suda, Ryota Tsurumi, Toshihito Shioya, Shinya Yoshida, Masanori Muroyama, Tomohiro Ishikawa, Hitoshi Shiku, Shiro Satoh, Masayoshi Esashi, Tomokazu Matsue

LAB ON A CHIP　15　(3)　848-856　2015

DOI： 10.1039/c4lc01099j 　

ISSN： 1473-0197

eISSN： 1473-0189
Metabolic suppression during mesodermal differentiation of embryonic stem cells identified by single-cell comprehensive gene expression analysis Peer-reviewed

Yuanshu Zhou, Ikuma Fujisawa, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

MOLECULAR BIOSYSTEMS　11　(9)　2560-2567　2015

DOI： 10.1039/c5mb00340g 　

ISSN： 1742-206X

eISSN： 1742-2051
Nanoscale visualization of redox activity at lithium-ion battery cathodes Peer-reviewed

Yasufumi Takahashi, Akichika Kumatani, Hirokazu Munakata, Hirotaka Inomata, Komachi Ito, Kosuke Ino, Hitoshi Shiku, Patrick R. Unwin, Yuri E. Korchev, Kiyoshi Kanamura, Tomokazu Matsue

NATURE COMMUNICATIONS　5　5450　2014/11

DOI： 10.1038/ncomms6450 　

ISSN： 2041-1723
Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique Peer-reviewed

Samad Ahadian, Shukuyo Yamada, Javier Ramon-Azcon, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

LAB ON A CHIP　14　(19)　3690-3694　2014/10

DOI： 10.1039/c4lc00479e 　

ISSN： 1473-0197

eISSN： 1473-0189
Hybrid hydrogels containing vertically aligned carbon nanotubes with anisotropic electrical conductivity for muscle myofiber fabrication Peer-reviewed

Samad Ahadian, Javier Ramon-Azcon, Mehdi Estili, Xiaobin Liang, Serge Ostrovidov, Hitoshi Shiku, Murugan Ramalingam, Ken Nakajima, Yoshio Sakka, Hojae Bae, Tomokazu Matsue, Ali Khademhosseini

SCIENTIFIC REPORTS　4　4271　2014/03

DOI： 10.1038/srep04271 　

ISSN： 2045-2322
Molecular Motor-Powered Shuttles along Multi-walled Carbon Nanotube Tracks Peer-reviewed

Aurelien Sikora, Javier Ramon-Azcon, Kyongwan Kim, Kelley Reaves, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Hitoshi Shiku, Tomokazu Matsue, Wonmuk Hwang, Winfried Teizer

NANO LETTERS　14　(2)　876-881　2014/02

DOI： 10.1021/nl4042388 　

ISSN： 1530-6984

eISSN： 1530-6992
Nanoscale visualisation of redox activity and Li+ (de)intercalation dynamics at LiFePO4 battery cathodes Peer-reviewed

Yasufumi Takahashi, Akichika Kumatani, Hirokazu Munakata, Hirotaka Inomata, Komachi Ito, Kosuke Ino, Hitoshi Shiku, Patrick R. Unwin, Yuri E. Korchev, Kiyoshi Kanamura, Tomokazu Matsue

Nature Commun　(5)　6450-6450　2014
Noninvasively measuring respiratory activity of rat primary hepatocyte spheroids by scanning electrochemical microscopy Peer-reviewed

Ryosuke Takahashi, Yuanshu Zhou, Yoshiko Horiguchi, Hitoshi Shiku, Hiroshi Sonoda, Naoshi Itabashi, Jiro Yamamoto, Taku Saito, Tomokazu Matsue, Akiko Hisada

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　117　(1)　113-121　2014/01

DOI： 10.1016/j.jbiosc.2013.06.021 　

ISSN： 1389-1723

eISSN： 1347-4421
Isolation and quantification of messenger RNA from tissue models by using a double-barrel carbon probe Peer-reviewed

Yuji Nashimoto, Yasufumi Takahashi, Ryosuke Takano, Kosuke Miyashita, Shukuyo Yamada, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL AND BIOANALYTICAL CHEMISTRY　406　(1)　275-282　2014/01

DOI： 10.1007/s00216-013-7430-z 　

ISSN： 1618-2642

eISSN： 1618-2650
Noninvasive Measurement of Alkaline Phosphatase Activity in Embryoid Bodies and Coculture Spheroids with Scanning Electrochemical Microscopy Peer-reviewed

Toshiharu Arai, Taku Nishijo, Yoshiharu Matsumae, Yuanshu Zhou, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　85　(20)　9647-9654　2013/10

DOI： 10.1021/ac401824q 　

ISSN： 0003-2700

eISSN： 1520-6882
Dielectrophoretically aligned carbon nanotubes to control electrical and mechanical properties of hydrogels to fabricate contractile muscle myofibers Peer-reviewed

Javier Ramón-Azcón, Samad Ahadian, Mehdi Estili, Xiaobin Liang, Serge Ostrovidov, Hirokazu Kaji, Hitoshi Shiku, Murugan Ramalingam, Ken Nakajima, Yoshio Sakka, Ali Khademhosseini, Tomokazu Matsue

Advanced Materials　25　(29)　4028-4034　2013/08/07

DOI： 10.1002/adma.201301300 　

ISSN： 0935-9648 1521-4095
Tacrolimus Inhibits the Revascularization of Isolated Pancreatic Islets Peer-reviewed

Nishimura, Ryuichi, Nishioka, Sho, Fujisawa, Ikuma, Shiku, Hitoshi, Shimada, Miki, Sekiguchi, Satoshi, Fujimori, Keisei, Ushiyama, Akira, Matsue, Tomokazu, Ohuchi, Noriaki, Satomi, Susumu, Goto, Masafumi

PLOS ONE　8　(4)　e56799　2013/04

DOI： 10.1371/journal.pone.0056799 　

ISSN： 1932-6203
Dielectrophoretically aligned carbon nanotubes to control electrical and mechanical properties of hydrogels for muscle tissue engineering Peer-reviewed

J. Ramón-Azcóna, S. Ahadian, M. Estili, X. Liang, S. Ostrovidov, H. Kaji, H. Shiku, M. Ramalingam, K. Nakajima, Y. Sakka, A. Khademhosseini, T. Matsue

Adv. Mater.　(25)　4028-4038　2013

DOI： 10.1002/adma.201301300 　
Evaluation of the differentiation status of single embryonic stem cells using scanning electrochemical microscopy Peer-reviewed

Yoshiharu Matsumae, Toshiharu Arai, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

CHEMICAL COMMUNICATIONS　49　(58)　6498-6500　2013

DOI： 10.1039/c3cc43126f 　

ISSN： 1359-7345
Noninvasive measurement of respiratory activity of mouse embryoid bodies and its correlation with mRNA levels of undifferentiation/differentiation markers Peer-reviewed

Hitoshi Shiku, Toshiharu Arai, Yuanshu Zhou, Nana Aoki, Taku Nishijo, Yoshiko Horiguchi, Kosuke Ino, Tomokazu Matsue

MOLECULAR BIOSYSTEMS　9　(11)　2701-2711　2013

DOI： 10.1039/c3mb70223e 　

ISSN： 1742-206X

eISSN： 1742-2051
Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene expression profiles Peer-reviewed

Yuanshu Zhou, Toshiharu Arai, Yoshiko Horiguchi, Kosuke Ino, Tomokazu Matsue, Hitoshi Shiku

Analytical Biochemistry　439　(2)　187-193　2013
Publisher: Academic Press Inc.
DOI： 10.1016/j.ab.2013.04.020 　

ISSN： 1096-0309 0003-2697
Novel Electrochemical Methodology for Activity Estimation of Alkaline Phosphatase Based on Solubility Difference Peer-reviewed

Kosuke Ino, Yusuke Kanno, Toshiharu Arai, Kumi Y. Inoue, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　84　(18)　7593-7598　2012/09

DOI： 10.1021/ac301429n 　

ISSN： 0003-2700
A new electrochemical assay method for gene expression using hela cells with a secreted alkaline phosphatase (SEAP) reporter system Peer-reviewed

Mustafa Sen, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

BIOTECHNOLOGY AND BIOENGINEERING　109　(8)　2163-2167　2012/08

DOI： 10.1002/bit.24461 　

ISSN： 0006-3592

eISSN： 1097-0290
Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopy Peer-reviewed

Yasufumi Takahashi, Andrew I. Shevchuk, Pavel Novak, Babak Babakinejad, Julie Macpherson, Patrick R. Unwin, Hitoshi Shiku, Julia Gorelik, David Klenerman, Yuri E. Korchev, Tomokazu Matsue

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　109　(29)　11540-11545　2012/07

DOI： 10.1073/pnas.1203570109 　

ISSN： 0027-8424
A Pt layer/Pt disk electrode configuration to evaluate respiration and alkaline phosphatase activities of mouse embryoid bodies Peer-reviewed

Raquel Obregon, Yoshiko Horiguchi, Toshiharu Arai, Shihomi Abe, Yuanshu Zhou, RyosukeTakahashi, Akiko Hisada, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

TALANTA　94　30-35　2012/05

DOI： 10.1016/j.talanta.2012.01.059 　

ISSN： 0039-9140

eISSN： 1873-3573
Development of an electrochemical Limulus amebocyte lysate assay technique for portable and highly sensitive endotoxin sensor Peer-reviewed

Kumi Y. Inoue, Satoko Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

INNATE IMMUNITY　18　(2)　343-349　2012/04

DOI： 10.1177/1753425911410337 　

ISSN： 1753-4259
Local Redox-Cycling-Based Electrochemical Chip Device with Deep Microwells for Evaluation of Embryoid Bodies Peer-reviewed

Kosuke Ino, Taku Nishijo, Toshiharu Arai, Yusuke Kanno, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　51　(27)　6648-6652　2012

DOI： 10.1002/anie.201201602 　

ISSN： 1433-7851
Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells Peer-reviewed

Javier Ramon-Azcon, Samad Ahadian, Raquel Obregon, Gulden Camci-Unal, Serge Ostrovidov, Vahid Hosseini, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

LAB ON A CHIP　12　(16)　2959-2969　2012

DOI： 10.1039/c2lc40213k 　

ISSN： 1473-0197

eISSN： 1473-0189
LSI-based amperometric sensor for bio-imaging and multi-point biosensing Peer-reviewed

Kumi Y. Inoue, Masahki Matsudaira, Reyushi Kubo, Masanori Nakano, Shinya Yoshida, Sakae Matsuzaki, Atsushi Suda, Ryota Kunikata, Tatsuo Kimura, Ryota Tsurumi, Toshihito Shioya, Kosuke Ino, Hitoshi Shiku, Shiro Satoh, Masayoshi Esashi, Tomokazu Matsue

LAB ON A CHIP　12　(18)　3481-3490　2012

DOI： 10.1039/c2lc40323d 　

ISSN： 1473-0197
Monitoring oxygen consumption of single mouse embryos using an integrated electrochemical microdevice Peer-reviewed

Yasumoto Date, Shinichiro Takano, Hitoshi Shiku, Kosuke Ino, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　30　(1)　100-106　2011/12

DOI： 10.1016/j.bios.2011.08.037 　

ISSN： 0956-5663
Electrochemical detection of receptor-mediated endocytosis by scanning electrochemical microscopy Peer-reviewed

Yasufumi Takahashi, Takeshi Miyamoto, Hitoshi Shiku, Kosuke Ino, Tomoyuki Yasukawa, Ryutaro Asano, Izumi Kumagai, Tomokazu Matsue

PHYSICAL CHEMISTRY CHEMICAL PHYSICS　13　(37)　16569-16573　2011

DOI： 10.1039/c1cp21886g 　

ISSN： 1463-9076

eISSN： 1463-9084
Multifunctional Nanoprobes for Nanoscale Chemical Imaging and Localized Chemical Delivery at Surfaces and Interfaces Peer-reviewed

Yasufumi Takahashi, Andrew I. Shevchuk, Pavel Novak, Yanjun Zhang, Neil Ebejer, Julie V. Macpherson, Patrick R. Unwin, Andrew J. Pollard, Debdulal Roy, Charles A. Clifford, Hitoshi Shiku, Tomokazu Matsue, David Klenerman, Yuri E. Korchev

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　50　(41)　9638-9642　2011

DOI： 10.1002/anie.201102796 　

ISSN： 1433-7851
Electrochemical chip integrating scalable ring-ring electrode array to detect secreted alkaline phosphatase Peer-reviewed

Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu Matsue

ANALYST　136　(23)　4991-4996　2011

DOI： 10.1039/c1an15620a 　

ISSN： 0003-2654

eISSN： 1364-5528
Chronoamperometric characterization of secreted alkaline phosphatase from single-cell entrapped in a poly(dimethylsiloxisane) microwell Peer-reviewed

Hitoshi Shiku, Junya Suzuki, Tatsuya Murata, Kosuke Ino, Tomokazu Matsue

ELECTROCHIMICA ACTA　55　(27)　8263-8267　2010/11

DOI： 10.1016/j.electacta.2010.04.011 　

ISSN： 0013-4686

eISSN： 1873-3859
Reporter gene expression at single-cell level characterized with real-time RT-PCR, chemiluminescence, fluorescence, and electrochemical imaging Peer-reviewed

Hitoshi Shiku, Daisuke Okazaki, Junya Suzuki, Yasufumi Takahashi, Tatsuya Murata, Hidetaka Akita, Hideyoshi Harashima, Kosuke Ino, Tomokazu Matsue

FEBS LETTERS　584　(18)　4000-4008　2010/09

DOI： 10.1016/j.febslet.2010.08.008 　

ISSN： 0014-5793
Simultaneous Noncontact Topography and Electrochemical Imaging by SECM/SICM Featuring Ion Current Feedback Regulation Peer-reviewed

Yasufumi Takahashi, Andrew I. Shevchuk, Pavel Novak, Yumi Murakami, Hitoshi Shiku, Yuri E. Korchev, Tomokazu Matsue

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　132　(29)　10118-10126　2010/07

DOI： 10.1021/ja1029478 　

ISSN： 0002-7863
Electrochemical Characterization of Enzyme and Immunoglobulin G Patterned Using Microcontact Printing Peer-reviewed

Hitoshi Shiku, Ayako Kumagai, Hong Qun Luo, Yasufumi Takahashi, Tomoyuki Yasukawa, Hiroshi Yamada, Tomokazu Matsue

ELECTROCHEMISTRY　78　(2)　122-125　2010/02

DOI： 10.5796/electrochemistry.78.122 　

ISSN： 1344-3542
Topographic imaging of convoluted surface of live cells by scanning ion conductance microscopy in a standing approach mode Peer-reviewed

Yasufumi Takahashi, Yumi Murakami, Kuniaki Nagamine, Hitoshi Shiku, Shigeo Aoyagi, Tomoyuki Yasukawa, Makoto Kanzaki, Tomokazu Matsue

PHYSICAL CHEMISTRY CHEMICAL PHYSICS　12　(34)　10012-10017　2010

DOI： 10.1039/c002607g 　

ISSN： 1463-9076
Electrochemical single-cell gene-expression assay combining dielectrophoretic manipulation with secreted alkaline phosphatase reporter system Peer-reviewed

Tatsuya Murata, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　25　(4)　913-919　2009/12

DOI： 10.1016/j.bios.2009.09.001 　

ISSN： 0956-5663

eISSN： 1873-4235
Transfected Single-Cell Imaging by Scanning Electrochemical Optical Microscopy with Shear Force Feedback Regulation Peer-reviewed

Yasufumi Takahashi, Hitoshi Shiku, Tatsuya Murata, Tomoyuki Yasukawa, Tomokazu Matsue

ANALYTICAL CHEMISTRY　81　(23)　9674-9681　2009/12

DOI： 10.1021/ac901796r 　

ISSN： 0003-2700
Electrochemical Detection of Epidermal Growth Factor Receptors on a Single Living Cell Surface by Scanning Electrochemical Microscopy Peer-reviewed

Yasufumi Takahashi, Takeshi Miyamoto, Hitoshi Shiku, Ryutaro Asano, Tomoyuki Yasukawa, Izumi Kumagai, Tomokazu Matsue

ANALYTICAL CHEMISTRY　81　(7)　2785-2790　2009/04

DOI： 10.1021/ac900195m 　

ISSN： 0003-2700

eISSN： 1520-6882
Development of electrochemical reporter assay using HeLa cells transfected with vector plasmids encoding various responsive elements Peer-reviewed

Hitoshi Shiku, Michiaki Takeda, Tatsuya Murata, Uichi Akiba, Fumio Hamada, Tomokazu Matsue

ANALYTICA CHIMICA ACTA　640　(1-2)　87-92　2009/04

DOI： 10.1016/j.aca.2009.03.018 　

ISSN： 0003-2670

eISSN： 1873-4324
A microfluidic dual capillary probe to collect messenger RNA from adherent cells and spheroids Peer-reviewed

Hitoshi Shiku, Takeshi Yamakawa, Yuji Nashimoto, Yasufumi Takahashi, Yu-suke Torisawa, Tomoyuki Yasukawa, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Hideki Kambara, Tomokazu Matsue

ANALYTICAL BIOCHEMISTRY　385　(1)　138-142　2009/02

DOI： 10.1016/j.ab.2008.10.039 　

ISSN： 0003-2697
Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system Peer-reviewed

Hitoshi Shiku, Shun Goto, Sungbong Jung, Kuniaki Nagamine, Masahiro Koide, Tomosato Itayama, Tomoyuki Yasukawa, Tomokazu Matsue

ANALYST　134　(1)　182-187　2009

DOI： 10.1039/b808428a 　

ISSN： 0003-2654

eISSN： 1364-5528
Electrochemical Gene-Function Analysis for Single Cells with Addressable Microelectrode/Microwell Arrays Peer-reviewed

Zhenyu Lin, Yasufumi Takahashi, Tatsttya Murata, Michiaki Takeda, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　48　(11)　2044-2046　2009

DOI： 10.1002/anie.200805743 　

ISSN： 1433-7851
An addressable microelectrode array for electrochemical detection Peer-reviewed

Zhenyu Lin, Yasufumi Takahashi, Yuusuke Kitagawa, Taizo Umemura, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　80　(17)　6830-6833　2008/09

DOI： 10.1021/ac801389d 　

ISSN： 0003-2700
Microfluidic chip integrated with amperometric detector array for in situ estimating oxygen consumption characteristics of single bovine embryos Peer-reviewed

Ching-Chou Wu, Takeshi Saito, Tomoyuki Yasukawa, Hitoshi Shiku, Hiroyuki Abe, Hiroyoshi Hoshi, Tornokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　125　(2)　680-687　2007/08

DOI： 10.1016/j.snb.2007.03.017 　

ISSN： 0925-4005
Regulation and characterization of the polarity of cells embedded in a reconstructed basement matrix using a three-dimensional micro-culture system Peer-reviewed

Yu-Suke Torisawa, Yuji Nashimoto, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

BIOTECHNOLOGY AND BIOENGINEERING　97　(3)　615-621　2007/06

DOI： 10.1002/bit.21274 　

ISSN： 0006-3592
Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy (SECM) Peer-reviewed

Kuniaki Nagamine, Shiho Onodera, Ai Kurihara, Tomoyuki Yasukawa, Hitoshi Shiku, Ryutaro Asano, Izumi Kumagai, Tomokazu Matsue

BIOTECHNOLOGY AND BIOENGINEERING　96　(5)　1008-1013　2007/04

DOI： 10.1002/bit.21173 　

ISSN： 0006-3592
A multicellular spheroid array to and viability realize spheroid formation, culture, assay on a chip Peer-reviewed

Yu-suke Torisawa, Airi Takagi, Yuji Nashimoto, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

BIOMATERIALS　28　(3)　559-566　2007/01

DOI： 10.1016/j.biomaterials.2006.08.054 　

ISSN： 0142-9612

eISSN： 1878-5905
Electrochemical monitoring of cellular signal transduction with a secreted alkaline phosphatase reporter system Peer-reviewed

Yu-suke Torisawa, Noriko Ohara, Kuniaki Nagamine, Shigenobu Kasai, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　78　(22)　7625-7631　2006/11

DOI： 10.1021/ac060737s 　

ISSN： 0003-2700
Oxygen permeability of surface-modified poly(dimethylsiloxane) characterized by scanning electrochemical microscopy Peer-reviewed

H Shiku, T Saito, CC Wu, T Yasukawa, M Yokoo, H Abe, T Matsue, H Yamada

CHEMISTRY LETTERS　35　(2)　234-235　2006/02

DOI： 10.1246/cl.2006.234 　

ISSN： 0366-7022

eISSN： 1348-0715
Oxygen consumption of cell suspension in a poly(dimethylsiloxane) (PDMS) microchannel estimated by scanning electrochemical microscopy Peer-reviewed

Takeshi Saito, Ching-Chou Wu, Hitoshi Shiku, Tomoyuki Yasukawa, Masaki Yokoo, Takashi Ito-Sasaki, Hiroyuki Abe, Hiroyoshi Hoshi, Tomokazu Matsue

ANALYST　131　(9)　1006-1011　2006

DOI： 10.1039/b600080k 　

ISSN： 0003-2654
Fabrication of miniature Clark oxygen sensor integrated with microstructure Peer-reviewed

CC Wu, T Yasukawa, H Shiku, T Matsue

SENSORS AND ACTUATORS B-CHEMICAL　110　(2)　342-349　2005/10

DOI： 10.1016/j.snb.2005.02.014 　

ISSN： 0925-4005
Real-time monitoring of reactive oxygen species production during differentiation of human monocytic cell lines (THP-1) Peer-reviewed

S Kasai, H Shiku, YS Torisawa, H Noda, J Yoshitake, T Shiraishi, T Yasukawa, T Watanabe, T Matsue, T Yoshimura

ANALYTICA CHIMICA ACTA　549　(1-2)　14-19　2005/09

DOI： 10.1016/j.aca.2005.06.034 　

ISSN： 0003-2670
Three-dimensional micro-culture system with a silicon-based cell array device for multi-channel drug sensitivity test Peer-reviewed

Y Torisawa, H Shiku, T Yasukawa, M Nishizawa, T Matsue

SENSORS AND ACTUATORS B-CHEMICAL　108　(1-2)　654-659　2005/07

DOI： 10.1016/j.snb.2004.11.045 　

ISSN： 0925-4005
Metabolic and enzymatic activities of individual cells, spheroids and embryos as a function of the sample size Peer-reviewed

H Shiku, YS Torisawa, A Takagi, S Aoyagi, H Abe, H Hoshi, T Yasukawa, T Matsue

SENSORS AND ACTUATORS B-CHEMICAL　108　(1-2)　597-602　2005/07

DOI： 10.1016/j.snb.2004.12.030 　

ISSN： 0925-4005
On-chip transformation of bacteria Peer-reviewed

K Nagamine, S Onodera, Y Torisawa, T Yasukawa, H Shiku, T Matsue

ANALYTICAL CHEMISTRY　77　(13)　4278-4281　2005/07

DOI： 10.1021/ac048278n 　

ISSN： 0003-2700
Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test Peer-reviewed

Y Torisawa, H Shiku, T Yasukawa, M Nishizawa, T Matsue

BIOMATERIALS　26　(14)　2165-2172　2005/05

DOI： 10.1016/j.biomaterials.2004.05.028 　

ISSN： 0142-9612
A Multicellular Spheroid-Based Drug Sensitivity Test by Scanning Electrochemical Microscopy Peer-reviewed

Y. Torisawa, A. Takagi, H. Shiku, T. Yasukawa, T. Matsue

Oncology Reports　13　(6)　1107-1112　2005/05
Respiration activity of single bovine embryos entrapped in a cone-shaped microwell monitored by scanning electrochemical microscopy Peer-reviewed

H Shiku, T Shiraishi, S Aoyagi, Y Utsumi, M Matsudaira, H Abe, H Hoshi, S Kasai, H Ohya, T Matsue

ANALYTICA CHIMICA ACTA　522　(1)　51-58　2004/09

DOI： 10.1016/j.aca.2004.06.054 　

ISSN： 0003-2670
Proliferation assay on a silicon chip applicable for tumors extirpated from mammalians Peer-reviewed

YS Torisawa, H Shiku, S Kasai, M Nishizawa, T Matsue

INTERNATIONAL JOURNAL OF CANCER　109　(2)　302-308　2004/03

DOI： 10.1002/ijc.11693 　

ISSN： 0020-7136
On-chip electrochemical measurement of beta-galactosidase expression using a microbial chip Peer-reviewed

T Kaya, K Nagamine, N Matsui, T Yasukawa, H Shiku, T Matsue

CHEMICAL COMMUNICATIONS　(2)　248-249　2004/01

DOI： 10.1039/b312462b 　

ISSN： 1359-7345
Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM) Peer-reviewed

T Kaya, D Numai, K Nagamine, S Aoyagi, H Shiku, T Matsue

ANALYST　129　(6)　529-534　2004

DOI： 10.1039/b316582e 　

ISSN： 0003-2654
Characterization of the peroxidase activity of single algae protoplasts by scanning electrochemical microscopy Peer-reviewed

HF Zhou, S Kasai, H Noda, H Ohya-Nishiguchi, H Shiku, T Matsue

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN　76　(9)　1757-1762　2003/09

DOI： 10.1246/bcsj.76.1757 　

ISSN： 0009-2673

eISSN： 1348-0634
Fabrication of microbial chip using collagen gel microstructure Peer-reviewed

T Kaya, K Nagamine, D Oyamatsu, H Shiku, M Nishizawa, T Matsue

LAB ON A CHIP　3　(4)　313-317　2003

DOI： 10.1039/b309349b 　

ISSN： 1473-0189
Oxygen consumption of single bovine embryos probed by scanning electrochemical microscopy Peer-reviewed

H Shiku, T Shiraishi, H Ohya, T Matsue, H Abe, H Hoshi, M Kobayashi

ANALYTICAL CHEMISTRY　73　(15)　3751-3758　2001/08

DOI： 10.1021/ac010339j 　

ISSN： 0003-2700
Monitoring activity of living cells entrapped in a membrane-sealed microcapillary with scanning electrochemical microscopy Peer-reviewed

H Skiku, T Shiraishi, H Ohya, T Matsue, H Abe, H Hoshi, M Kobayashi

ELECTROCHEMISTRY　68　(11)　890-892　2000/11

ISSN： 1344-3542
Near-field scanning optical microscopy studies of L-alpha-dipalmitoylphosphatidylcholine monolayers at the air/liquid interface Peer-reviewed

H. Shiku, R. C. Dunn

J. Microscopy　194　461-466　1999/08
Domain formation in thin lipid films probed with near-field scanning optical microscopy Peer-reviewed

H. Shiku, R. C. Dunn

J. Microscopy　194　455-460　1999/08

DOI： 10.1046/j.1365-2818.1999.00526.x 　
Noncontact near-field scanning optical microscopy imaging using an interferometric optical feedback mechanism Peer-reviewed

H. Shiku, J. R. Krogmeier, R. C. Dunn

Langmuir　15　2162-2168　1999/08

DOI： 10.1021/la981198s 　
Direct observation of DPPC domain motion on mica surface under conditions of high relative humidity Peer-reviewed

H. Shiku, R. C. Dunn

J. Phys. Chem. B　102　3791-3797　1998/03

DOI： 10.1021/jp9808330 　
Dual immunoassay of human chorionic gonadotropin and human placental lactogen at a miocrofabricated substrate by scanning electrochemical microscopy Peer-reviewed

Hitoshi Shiku, Yoshitake Hara, Tomokazu Matsue, Isamu Uchida, Tadakazu Yamauchi

Journal of Electroanalytical Chemistry　438　(1-2)　187-190　1997/11/25
Publisher: Elsevier
DOI： 10.1016/S0022-0728(96)04979-0 　

ISSN： 1572-6657
Microfabrication of alkylsilanized glass substrate by electrogenerated hydroxyl radical using scanning electrochemical microscopy Peer-reviewed

H. Shiku, I. Uchida, T. Matsue

Langmuir　13　7239-7244　1997/10

DOI： 10.1021/la970554o 　
Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy Peer-reviewed

Hitoshi Shiku, Tomokazu Matsue, Isamu Uchida

Analytical Chemistry　68　(7)　1276-1278　1996
Publisher: American Chemical Society
DOI： 10.1021/ac950824c 　

ISSN： 0003-2700
MICROFABRICATION AND CHARACTERIZATION OF DIAPHORASE-PATTERNED SURFACES BY SCANNING ELECTROCHEMICAL MICROSCOPY Peer-reviewed

H SHIKU, T TAKEDA, H YAMADA, T MATSUE, UCHIDA, I

ANALYTICAL CHEMISTRY　67　(2)　312-317　1995/01

DOI： 10.1021/ac00098a014 　

ISSN： 0003-2700
Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry Peer-reviewed

Tomokazu Matsue, Hitoshi Shiku, Hiroshi Yamada, Isamu Uchida

Journal of Physical Chemistry　98　(43)　11001-11003　1994

DOI： 10.1021/j100094a002 　

ISSN： 0022-3654
Analysis for diffusion of redox species in 3-dimensional cultured cells using microelectrodes

Shotaro Yamada, Gaku Kurogi, Keita Ebine, Tomoyuki Ogawa, Yuanjia Lin, Kosuke Ino, Shan Liu, Chen-zhong Li, Hitoshi Shiku, Hiroya Abe

Electrochimica Acta　2025/10

DOI： 10.1016/j.electacta.2025.147005 　
Electrochemiluminescence microscopy of diffusive biocompounds as co-reactants in cell spheroids with [Ru(bpy)3]2+

Tomas Mockaitis, Ryota Shikuwa, Yoshinobu Utagawa, Kimiharu Oba, Kaoru Hiramoto, Hiroya Abe, Inga Morkvenaite-Vilkonciene, Hitoshi Shiku, Kosuke Ino

Sensors and Actuators B: Chemical　2025/10

DOI： 10.1016/j.snb.2025.137944 　
Detection of C‐Reactive Protein by Electrochemiluminescence‐Based Droplet‐Free Digital Immunoassay with Tyramide Signal Amplification

Hiroya Abe, Shuri Nakamura, Kentaro Ito, Kosuke Ino, Zhengyi Liu, Shinichi Sato, Kumi Y. Inoue, Hitoshi Shiku

ChemElectroChem　2025/06/10

DOI： 10.1002/celc.202500024 　
Hydrogen Binding Energy Is Insufficient for Describing Hydrogen Evolution on Single‐Atom Catalysts Peer-reviewed

Songbo Ye, Fangzhou Liu, Fangxin She, Jiaxiang Chen, Di Zhang, Akichika Kumatani, Hitoshi Shiku, Li Wei, Hao Li

Angewandte Chemie International Edition　2025/03/20
Publisher: Wiley
DOI： 10.1002/anie.202425402 　

ISSN： 1433-7851

eISSN： 1521-3773

More details Close

The design principles for metal‐nitrogen‐carbon (M‐N‐C) single‐atom catalysts (SACs) in the hydrogen evolution reaction (HER) have been extensively studied. While hydrogen binding energy ([[EQUATION]]) has long been used as a HER descriptor during the past decades, its applicability to HER SACs has been met with significant controversy. Herein, we investigate the effects of HO*/O* poisoning and H* coverage on SACs with varied metal centers and coordination environments using pH‐dependent surface Pourbaix diagrams at the reversible hydrogen electrode (RHE) scale and microkinetic modeling. Our findings reveal that HO* poisoning, realistic H* adsorption strengths at active metal sites, and the potential HER activity at the coordinating N‐sites are crucial factors that should be considered for accurate descriptor development. Experimental validation using a series of M‐phthalocyanine/CNT catalysts confirms the theoretical predictions, with excellent agreement in exchange current densities and the role of N‐sites in Ni/Cu‐phthalocyanine/CNT catalysts. More importantly, the controversy surrounding HER SAC design principles is resolved through a novel 2D microkinetic volcano model that incorporates active sites, H* coverage, and HO* poisoning. This work provides answers to a long‐lasting debate on HER descriptors by establishing [[EQUATION]] and [[EQUATION]] as a combined HER descriptor for SACs, offering new guidelines for catalyst design.
EMT-Induced Morphological Variations on Living Cell Membrane Surface Peer-reviewed

Hiroki Ida, Noriko Taira, Yuji Nashimoto, Akichika Kumatani, Yasufumi Takahashi, Hitoshi Shiku

Analytical Chemistry　97　(1)　312-318　2025/01/14

DOI： 10.1021/acs.analchem.4c04204 　
Electrochemiluminescence of [Ru(bpy)3]2+/tri-n-propylamine to visualize different lipid compositions in supported lipid membranes

Kaoru Hiramoto, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku

Chemical Communications　2025

DOI： 10.1039/D4CC06245K 　
Extended Spherical Diffusion Theory: Electrochemiluminescence Imaging Analysis of Diffusive Molecules from Spherical Biosamples

Kosuke Ino, Miyu Mashiko, Yusuke Kanno, Yeyi Tang, Shuzo Masui, Takasi Nisisako, Kaoru Hiramoto, Hiroya Abe, Hitoshi Shiku

Analytical Chemistry　2024/12/03

DOI： 10.1021/acs.analchem.4c03167 　
Porous membranes integrated into electrochemical systems for bioanalysis Peer-reviewed

Kosuke Ino, Yoshinobu Utagawa, Kaoru Hiramoto, Hiroya Abe, Hitoshi Shiku

Electrochemical Science Advances　4　e2300026　2024/12

DOI： 10.1002/elsa.202300026 　
(Invited) Development of Bipolar Electrochemical Microscopy for High-Speed and High-Resolution Monitoring of Biochemical Phenomena

Kumi Y. Inoue, Koki Kubota, Ayane Endo, Tomoki Iwama, Takahiro Ito-Sasaki, Hitoshi Shiku

ECS Meeting Abstracts　MA2024-02　(65)　4343-4343　2024/11/22
Publisher: The Electrochemical Society
DOI： 10.1149/ma2024-02654343mtgabs 　

eISSN： 2151-2043

More details Close

Closed bipolar electrochemistry (cBPE) involves simultaneous oxidation and reduction at opposite poles of a conductor in two separated solutions. (Figure left). The unique property of cBPE to be operated without direct connection to an external power source is expected to lead to higher resolution in electrochemical imaging than the conventional microelectrode array type imaging platforms. We have applied cBPE to an electrochemical microscopy (BEM) (Figure right) for high-resolution biochemical imaging.^1- ⁷ The operation principle of BEM has been successfully verified,¹ then the current focus is on fabrication method of cBPE array. Previously, we filled Au into pores of a polyethylene terephthalate track-etched membrane having D=8 µm pores by electroless plating using 50 mM HAuCl₄ aqueous solution and 50 mM NaBH₄ EtOH solution.² Using this cBPE array, an image of oxygen concentration distribution around cell spheroids was successfully obtained, but this method has a problem of the inability to completely fill the pores with gold, resulting in large ionic currents. In this study, we use conductive pastes for fabrication of vertical closed bipolar electrode array to completely fill the pores with conductive materials. Epoxy-based carbon paste (ECP) was prepared by mixing acetylene carbon black (ACB; particle size=42 nm; Strem Chemicals) and epoxy resin (mixture of Epok 812 (62 mL), DDSA (100 mL), and DMP-30 (1.5%); Okenshoji). Oil-based carbon paste (OCP) was prepared by mixing the ACB and the liquid paraffin. For Au paste (AuP), commercially available AURoFUSE (particle size (D50) 0.2–0.4 µm; Tanaka Kikinzoku Kogyo) was used as is. Vertical cBPE arrays using the carbon paste filled in pores of a glass capillary plate was fabricated by filling ECP (ACB: epoxy resin=1:4 in weight ratio) cured at 60°C for 15 h. After cooling, the surface was coated by OCP (ACB: liquid paraffin=1:1 in weight ratio) to fill the dented parts shrank by heat curing. For [Fe(CN)₆]³ ⁻ imaging, a sample side of the array was modified with reduced graphene oxide (RGO) by coating OCP containing 5 wt.% RGO (RGO-OCP). For H₂O₂ imaging, a sample side of the array was modified with Prussian blue (PB) by coating commercially available PB containing carbon paste (Carbon Mediator Paste, C2070424P2; SunChemical Gwent). The BEM system was constructed using two solution chambers (detection/sample chambers), BPE array, and driving electrodes. The detection chamber located at the bottom side of the BPE array was filled with 10 mM [Ru(bpy)₃] Cl₂/20 mM tripropylamine (TPA) solution containing phosphate buffered saline (PBS). The sample chamber located at the bottom side was filled with the sample solution for each measurement. A platinum driving electrode inserted into the detection chamber, another platinum driving electrode and a Ag/AgCl reference electrode inserted into the sample chamber were connected WE, CE and RE connector of a potentiostat, respectively. The entire system was mounted on an inverted microscope with a high sensitivity CCD camera to observe the electrochemiluminescence (ECL) generated at the underside of the BPEs. By using RGO and PB modified carbon paste (PC) cBPE array for BEM element, we successfully obtained an image of 5 mM [Fe(CN)₆]³⁻ and H₂O₂ inflow, respectively.⁵ These results show that cBPE arrays for BEM can be fabricated by filling micropores with carbon paste taking advantage of the flexibility and ease of modification of the paste electrode. We are now investigating the use of AuP as an electrode material of cBPE array. We have obtained the result that ECL can be enhanced by using AuP as an electrode material.⁷ This may be due to the surface plasmon enhancement effect by the Au particles contained in the AuP. The establishment of a simple method for fabricating vertical cBPE arrays without ion leakage is expected to further accelerate the research and development of BEM. References: Iwama, K. Y. Inoue, H. Abe, T. Matsue, Chem. Lett., 47, 843–845 (2018) Iwama, K. Y. Inoue, H. Abe, T. Matsue, H. Shiku, Analyst, 145, 6895–6900 (2020) Iwama, M. Komatsu, K. Y. Inoue, H. Shiku, ChemElectroChem, 8, 3492–3498 (2021) Iwama, Y. Guo, S. Handa, K. Y. Inoue, T. Yoshinobu, F. Sorin, H. Shiku, Adv. Mater. Technol., 7, 2101066–2101066 (2022) Iwama, K. Y. Inoue, H. Shiku, Anal. Chem., 94, 8857–8866 (2022) Iwama, M. Komatsu, K. Y. Inoue, K. Kubota, T. Ito-Sasaki, H. Shiku, SSRN, posted 28 Nov 2023, available at https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4632693 A. Endo, K. Kubota, T. Ito-Sasaki, M. Komatsu, T. Iwama, H. Shiku, K. Y. Inoue, Electrochemistry, 92, 022013 (2024). Figure 1
Electrochemical permeability assays of hydrolyzed acetylsalicylic acid (aspirin) in engineered gut models Peer-reviewed

Chisato Ito, Kosuke Ino, Yoshinobu Utagawa, Kazuyuki Iwase, Yasuhiko Shinoda, Hiroya Abe, Hitoshi Shiku

Chemistry Letters　53　(9)　2024/08/30
Publisher: Oxford University Press (OUP)
DOI： 10.1093/chemle/upae174 　

ISSN： 0366-7022

eISSN： 1348-0715

More details Close

Abstract This study presents an electrochemical permeability assay for drugs using an engineered gut model. Caco-2 cells (human colorectal adenocarcinoma cell line) were cultured on porous membranes for 21 d. Acetylsalicylic acid (aspirin), after hydrolysis, was introduced from either the apical or basal sides and incubated for 2 h. The solution from the acceptor side was collected, and cyclic voltammetry was conducted to measure the hydrolyzed acetylsalicylic acid. This electrochemical approach holds promise for drug screening applications in engineered gut models.
Fabrication of Two-Layer Microfluidic Devices with Porous Electrodes Using Printed Sacrificial Layers Peer-reviewed

Kosuke Ino, An Konno, Yoshinobu Utagawa, Taiyo Kanno, Kazuyuki Iwase, Hiroya Abe, Hitoshi Shiku

Micromachines　15　(8)　1054-1054　2024/08/22
Publisher: MDPI AG
DOI： 10.3390/mi15081054 　

eISSN： 2072-666X

More details Close

Two-layer microfluidic devices with porous membranes have been widely used in bioapplications such as microphysiological systems (MPS). Porous electrodes, instead of membranes, have recently been incorporated into devices for electrochemical cell analysis. Generally, microfluidic channels are prepared using soft lithography and assembled into two-layer microfluidic devices. In addition to soft lithography, three-dimensional (3D) printing has been widely used for the direct fabrication of microfluidic devices because of its high flexibility. However, this technique has not yet been applied to the fabrication of two-layer microfluidic devices with porous electrodes. This paper proposes a novel fabrication process for this type of device. In brief, Pluronic F-127 ink was three-dimensionally printed in the form of sacrificial layers. A porous Au electrode, fabricated by sputtering Au on track-etched polyethylene terephthalate membranes, was placed between the top and bottom sacrificial layers. After covering with polydimethylsiloxane, the sacrificial layers were removed by flushing with a cold solution. To the best of our knowledge, this is the first report on the sacrificial approach-based fabrication of two-layer microfluidic devices with a porous electrode. Furthermore, the device was used for electrochemical assays of serotonin and could successfully measure concentrations up to 5 µM. In the future, this device can be used for MPS applications.
Enzyme-Free In-Situ Electrochemical Measurement Using a Porous Membrane Electrode for Glucose Transport into Cell Spheroids Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Yasuhiko Shinoda, Masateru Yamazaki, Hiroya Abe, Hitoshi Shiku

ACS Sensors　9　(8)　4248-4255　2024/07/30
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acssensors.4c01230 　

ISSN： 2379-3694

eISSN： 2379-3694
膵臓移植・膵島移植・人工膵臓の現状と展望生体模倣システムによる膵島の血管形成能力と遺伝子発現の照合

梨本裕司, 今野杏, 西川香里, 伊野浩介, 堀武志, 梶弘和, 新宅博文, 後藤昌史, 珠玖仁

膵臓　39　(3)　A279-A279　2024/07
Publisher: (一社)日本膵臓学会
ISSN： 0913-0071

eISSN： 1881-2805
膵臓移植・膵島移植・人工膵臓の現状と展望生体模倣システムによる膵島の血管形成能力と遺伝子発現の照合

梨本裕司, 今野杏, 西川香里, 伊野浩介, 堀武志, 梶弘和, 新宅博文, 後藤昌史, 珠玖仁

膵臓　39　(3)　A279-A279　2024/07
Publisher: (一社)日本膵臓学会
ISSN： 0913-0071

eISSN： 1881-2805
Comprehensive electrochemical imaging analyses of redox activities correlated to multilayer graphene and graphite structures Peer-reviewed

Akichika Kumatani, Chiho Miura, Hiroki Ida, Yasufumi Takahashi, Yuichi Ikuhara, Hitoshi Shiku, Tomokazu Matsue, Takeru Okada

Electrochimica Acta　499　144688-144688　2024/07
Publisher: Elsevier BV
DOI： 10.1016/j.electacta.2024.144688 　

ISSN： 0013-4686
Bipolar Electrochemical Sensor with Perylene Diimide-Based Cathodic Luminophore for Dopamine Detection and Imaging Peer-reviewed

Kumi Y. Inoue, Tomoki Iwama, Mayo Komatsu, Koki Kubota, Takahiro Ito-Sasaki, Hitoshi Shiku

Talanta　278　126509　2024/07
Publisher: Elsevier BV
DOI： 10.2139/ssrn.4632693 　
Scanning electrochemical microscopy for determining oxygen consumption rates of cells in hydrogel fibers fabricated using an extrusion 3D bioprinter Peer-reviewed

Kosuke Ino, Mana Wachi, Yoshinobu Utagawa, An Konno, Masahiro Takinoue, Hiroya Abe, Hitoshi Shiku

Analytica Chimica Acta　1304　342539-342539　2024/05
Publisher: Elsevier BV
DOI： 10.1016/j.aca.2024.342539 　

ISSN： 0003-2670
Comprehensive Cell Adhesion Analysis Using Electrochemiluminescence Imaging and Electrochemical Impedance Spectroscopy Peer-reviewed

Kimiharu OBA, Kosuke INO, Yoshinobu UTAGAWA, Hiroya ABE, Hitoshi SHIKU

Electrochemistry　92　(2)　022009　2024/05
Publisher: The Electrochemical Society of Japan
DOI： 10.5796/electrochemistry.23-68109 　

ISSN： 1344-3542

eISSN： 2186-2451
Enhancement of Electrochemiluminescence by Au Paste Electrode for Bipolar Electroanalysis Peer-reviewed

Ayane ENDO, Koki KUBOTA, Takahiro ITO-SASAKI, Mayo KOMATSU, Tomoki IWAMA, Hitoshi SHIKU, Kumi Y. INOUE

Electrochemistry　92　(2)　022013　2024/05
Publisher: The Electrochemical Society of Japan
DOI： 10.5796/electrochemistry.23-68114 　

ISSN： 1344-3542

eISSN： 2186-2451
Osteogenic differentiation of mesenchymal stem cell spheroids: A microfluidic device and electrochemiluminescence imaging study Peer-reviewed

Kaoru Hiramoto, An Konno, Yuji Nashimoto, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku

Electrochimica Acta　491　144291-144291　2024/04
Publisher: Elsevier BV
DOI： 10.1016/j.electacta.2024.144291 　

ISSN： 0013-4686
Emergence of electrochemical catalytic activity via an electrochemical-probe on defective transition metal dichalcogenide nanosheets Peer-reviewed

A. Kumatani, H. Ogawa, T. Endo, J. Lustikova, H. Ida, Y. Takahashi, Y. Miyata, Y. Ikuhara, H. Shiku, Y. Wakayama

APL Energy　2　016107　2024/03/01

DOI： 10.1063/5.0175653 　
Electrochemiluminescence microscopy for the investigation of peptides interactions within planar lipid membranes Peer-reviewed

Kaoru Hiramoto, Kosuke Ino, Ibuki Takahashi, Ayumi Hirano-Iwata, Hitoshi Shiku

Faraday Discussions　2024
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d4fd00137k 　

ISSN： 1359-6640

eISSN： 1364-5498

More details Close

Understanding the interactions between lipid membranes and peptides is crucial for controlling bacterial and viral infections, and developing effective drugs. In this study, we proposed the use of electrochemiluminescence (ECL)...
Microfluidic vascular formation model for assessing angiogenic capacities of single islets. International-journal Peer-reviewed

Yuji Nashimoto, An Konno, Takuto Imaizumi, Kaori Nishikawa, Kosuke Ino, Takeshi Hori, Hirokazu Kaji, Hirofumi Shintaku, Masafumi Goto, Hitoshi Shiku

Biotechnology and bioengineering　121　(3)　1050-1059　2024/01/01

DOI： 10.1002/bit.28631 　

More details Close

Pancreatic islet transplantation presents a promising therapy for individuals suffering from type 1 diabetes. To maintain the function of transplanted islets in vivo, it is imperative to induce angiogenesis. However, the mechanisms underlying angiogenesis triggered by islets remain unclear. In this study, we introduced a microphysiological system to study the angiogenic capacity and dynamics of individual islets. The system, which features an open-top structure, uniquely facilitates the inoculation of islets and the longitudinal observation of vascular formation in in vivo like microenvironment with islet-endothelial cell communication. By leveraging our system, we discovered notable islet-islet heterogeneity in the angiogenic capacity. Transcriptomic analysis of the vascularized islets revealed that islets with high angiogenic capacity exhibited upregulation of genes related to insulin secretion and downregulation of genes related to angiogenesis and fibroblasts. In conclusion, our microfluidic approach is effective in characterizing the vascular formation of individual islets and holds great promise for elucidating the angiogenic mechanisms that enhance islet transplantation therapy.
Vasculature-on-a-Chip with a Porous Membrane Electrode for In Situ Electrochemical Detection of Nitric Oxide Released from Endothelial Cells Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Kazuyuki Iwase, Yuji Nashimoto, Itaru Honma, Hitoshi Shiku

Analytical Chemistry　95　(49)　18158-18165　2023/11/28
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.3c03684 　

ISSN： 0003-2700

eISSN： 1520-6882
C-reactive Protein Detection Using an Ion-sensitive Field-effect Transistor (ISFET)-based Aptasensor with a Chemically Modified Gate Surface for Improved Sensitivity Peer-reviewed

Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Masayoshi Esashi, Hitoshi Shiku

Sensors and Materials　35　(10)　4797-4808　2023/10/25
Publisher: MYU K.K.
DOI： 10.18494/sam4570 　

ISSN： 0914-4935

eISSN： 2435-0869
Electrochemical imaging correlated to hydrogen evolution reaction on transition metal dichalcogenide, WS2 Peer-reviewed

Akichika Kumatani, Hiroto Ogawa, Takahiko Endo, Yu Kobayashi, Jana Lustikova, Hiroki Ida, Yasufumi Takahashi, Tomokazu Matsue, Yasumitsu Miyata, Hitoshi Shiku

Journal of Vacuum Science & Technology B　41　(5)　052401　2023/09/01

DOI： 10.1116/6.0002706 　
Energy-harvesting and mass sensor performances of magnetostrictive cobalt ferrite-spattered Fe–Co alloy plate Peer-reviewed

Hiroki Kurita, Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Takeru Nakaki, Shotaro Kuroda, Zhenjin Wang, Wakako Araki, Hitoshi Shiku, Fumio Narita

Journal of Alloys and Compounds　951　169844-169844　2023/08
Publisher: Elsevier BV
DOI： 10.1016/j.jallcom.2023.169844 　

ISSN： 0925-8388
Fabrication and Cell Culture Applications of Core-Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations. International-journal Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Masahiro Takinoue, Hitoshi Shiku

Advanced healthcare materials　12　(31)　e2302011　2023/07/21

DOI： 10.1002/adhm.202302011 　

More details Close

Core-shell hydrogel fibers are widely used in cell culture applications. A simple and rapid method is presented for fabricating core-shell hydrogel fibers, consisting of straight or beaded core fibers, for cell culture applications. The core fibers are prepared using interfacial polyelectrolyte complexation (IPC) with chitosan and DNA. Briefly, two droplets of chitosan and DNA are brought in contact to form an IPC film, which is dragged to prepare an IPC fiber. The incubation time and DNA concentration are adjusted to prepare straight and beaded IPC fibers. The fibers with Ca2+ are immersed in an alginate solution to form calcium alginate shell hydrogels around the core IPC fibers. To the best of the knowledge, this is the first report of core-shell hydrogel fibers with IPC fiber cores. To demonstrate cell culture, straight hydrogel fibers are applied to fabricate hepatic models consisting of HepG2 and 3T3 fibroblasts, and vascular models consisting of human umbilical vein endothelial cells and 3T3 fibroblasts. To evaluate the effect of co-culture, albumin secretion, and angiogenesis are evaluated. Beaded hydrogel fibers are used to fabricate many size-controlled spheroids for fiber and cloning applications. This method can be widely applied in tissue engineering and cell analysis.
Microarray-Based Electrochemical Biosensing Peer-reviewed

Kosuke Ino, Yoshinobu Utagawa, Hitoshi Shiku

Advances in Biochemical Engineering/Biotechnology　187　317-338　2023/06/13
Publisher: Springer Berlin Heidelberg
DOI： 10.1007/10_2023_229 　

ISSN： 0724-6145

eISSN： 1616-8542
Oxygen metabolism analysis of a single organoid toward the noninvasive discrimination of cancer subpopulations with different growth capabilities International-journal Peer-reviewed

Yuji Nashimoto, Shotaro Shishido, Kunishige Onuma, Kosuke Ino, Masahiro Inoue, Hitoshi Shiku

Frontiers in Bioengineering and Biotechnology　11　1184325-1184325　2023/05

DOI： 10.3389/fbioe.2023.1184325 　

More details Close

Heterogeneous nature is a pivotal aspect of cancer, rendering treatment problematic and frequently resulting in recurrence. Therefore, advanced techniques for identifying subpopulations of a tumour in an intact state are essential to develop novel screening platforms that can reveal differences in treatment response among subpopulations. Herein, we conducted a non-invasive analysis of oxygen metabolism on multiple subpopulations of patient-derived organoids, examining its potential utility for non-destructive identification of subpopulations. We utilised scanning electrochemical microscopy (SECM) for non-invasive analysis of oxygen metabolism. As models of tumours with heterogeneous subpopulations, we used patient-derived cancer organoids with a distinct growth potential established using the cancer tissue-originated spheroid methodology. Scanning electrochemical microscopy measurements enabled the analysis of the oxygen consumption rate (OCR) for individual organoids as small as 100 µm in diameter and could detect the heterogeneity amongst studied subpopulations, which was not observed in conventional colorectal cancer cell lines. Furthermore, our oxygen metabolism analysis of pre-isolated subpopulations with a slow growth potential revealed that oxygen consumption rate may reflect differences in the growth rate of organoids. Although the proposed technique currently lacks single-cell level sensitivity, the variability of oxygen metabolism across tumour subpopulations is expected to serve as an important indicator for the discrimination of tumour subpopulations and construction of novel drug screening platforms in the future.
局所電気化学測定を可能とするナノ電気化学セル顕微鏡とその応用

明哉熊谷, 覚仁佐藤, 佳子堀口, 仁珠玖

顕微鏡　58　(1)　13-17　2023/04/30

DOI： 10.11410/kenbikyo.58.1_13 　

ISSN： 1349-0958 2434-2386
Simple, Rapid, and Large‐Scale Fabrication of Multi‐Branched Hydrogels Based on Viscous Fingering for Cell Culture Applications Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Hitoshi Shiku

Macromolecular Bioscience　2300069　2023/04/22
Publisher: Wiley
DOI： 10.1002/mabi.202300069 　

ISSN： 1616-5187

eISSN： 1616-5195
ミニ組織モデルに対する経血管刺激の電気化学的定量評価

梨本裕司, 堀武志, 梶弘和, 伊野浩介, 珠玖仁

生体医歯工学共同研究拠点成果報告書　2022年度　109-109　2023/04
Publisher: 東京医科歯科大学生体材料工学研究所
ミニ組織モデルに対する経血管刺激の電気化学的定量評価

梨本裕司, 堀武志, 梶弘和, 伊野浩介, 珠玖仁

生体医歯工学共同研究拠点成果報告書　2022年度　109-109　2023/04
Publisher: 東京医科歯科大学生体材料工学研究所
Effects of Ion Concentration on Ion Current Rectification Using a Glass Nanocapillary Peer-reviewed

Chisato ITO, Kosuke INO, Kaoru HIRAMOTO, Yuji NASHIMOTO, Hitoshi SHIKU

BUNSEKI KAGAKU　72　(3)　117-123　2023/03/05
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.72.117 　

ISSN： 0525-1931
Topographical evaluation of human mesenchymal stem cells during osteogenic differentiation using scanning ion conductance microscopy Peer-reviewed

Kota Nozawa, Xuyang Zhang, Takuo Nakamura, Yuji Nashimoto, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku

Electrochimica Acta　449　142192　2023/03

DOI： 10.1016/j.electacta.2023.142192 　
Surface morphology live-cell imaging reveals how macropinocytosis inhibitors affect membrane dynamics Peer-reviewed

Hiroki Ida, Noriko Taira, Koichi Azuma, Akichika Kumatani, Misao Akishiba, Shiroh Futaki, Yasufumi Takahashi, Hitoshi Shiku

Electrochimica Acta　441　141783-141783　2023/02
Publisher: Elsevier BV
DOI： 10.1016/j.electacta.2022.141783 　

ISSN： 0013-4686
Droplet-free digital immunoassay based on electrochemiluminescence Peer-reviewed

Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku

Biosensors and Bioelectronics: X　13　100312-100312　2023/02
Publisher: Elsevier BV
DOI： 10.1016/j.biosx.2023.100312 　

ISSN： 2590-1370
Highly Sensitive Electrochemical Endotoxin Sensor Based on Redox Cycling Using an Interdigitated Array Electrode Device. International-journal Peer-reviewed

Kentaro Ito, Kumi Y Inoue, Takahiro Ito-Sasaki, Miho Ikegawa, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku

Micromachines　14　(2)　2023/01/27

DOI： 10.3390/mi14020327 　

More details Close

The Limulus amebocyte lysate (LAL) reaction-based assay, the most commonly used endotoxin detection method, requires a skilled technician. In this study, to develop an easy-to-use and highly sensitive endotoxin sensor, we created an electrochemical endotoxin sensor by using an interdigitated array electrode (IDAE) device with advantages of amplifiable signals via redox cycling and portability. We added Boc-Leu-Gly-Arg-p-aminophenol (LGR-pAP) as an electrochemical substrate for an LAL reaction and detected p-aminophenol (pAP) released from LGR-pAP as a product of an endotoxin-induced LAL reaction via an IDAE device. The IDAE device showed a great redox cycling efficiency of 79.8%, and a 4.79-fold signal amplification rate. Then, we confirmed that pAP was detectable in the presence of LGR-pAP through chronoamperometry with the potential of the anode stepped from -0.3 to 0.5 V vs. Ag/AgCl while the cathode was biased at -0.3 V vs. Ag/AgCl. Then, we performed an endotoxin assay by using the IDAE device. Our endotoxin sensor detected as low as 0.7 and 1.0 endotoxin unit/L after the LAL reaction for 1 h and 45 min, respectively, and these data were within the cut-off value for ultrapure dialysis fluid. Therefore, our highly sensitive endotoxin sensor is useful for ensuring medical safety.
Evaluation of respiratory and secretory activities of multicellular spheroids via electrochemiluminescence imaging International-journal Invited Peer-reviewed

Kaoru Hiramoto, Keika Komatsu, Ryota Shikuwa, An Konno, Yusuke Sato, Ayumi Hirano-Iwata, Kosuke Ino, Hitoshi Shiku

Electrochimica Acta　458　142507-142507　2023
Publisher: Elsevier BV
DOI： 10.1016/j.electacta.2023.142507 　

ISSN： 0013-4686
Batteryless wireless magnetostrictive Fe30Co70/Ni clad plate for human coronavirus 229E detection Peer-reviewed

Daiki Neyama, Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Hiroki Kurita, Shion Osana, Naoto Miyamoto, Tsuyoki Tayama, Daiki Chiba, Masahito Watanabe, Hitoshi Shiku, Fumio Narita

Sensors and Actuators A: Physical　349　114052-114052　2023/01
Publisher: Elsevier BV
DOI： 10.1016/j.sna.2022.114052 　

ISSN： 0924-4247
High-Sensitivity Amperometric Dual Immunoassay Using Two Cascade Reactions with Signal Amplification of Redox Cycling in Nanoscale Gap International-journal Peer-reviewed

Kentaro Ito, Kumi Y Inoue, Kosuke Ino, Hitoshi Shiku

Analytical Chemistry　94　(47)　16451-16460　2022/11/04
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.2c03921 　

ISSN： 0003-2700

eISSN： 1520-6882

More details Close

Here, we report a high-sensitivity dual immunoassay using Lumulus amebocyte lysate (LAL) and blood coagulation cascade reactions with redox cycling in a nanoscale-gap electrode. Endotoxin and factor XIa were used as the label molecules for the immunoassay of two types of analytes to induce the LAL and coagulation cascade reactions, respectively, when each corresponding analyte existed in the sample solution. In addition to the signal amplification by the cascade reactions, we employed redox cycling in a nanoscale gap to achieve a highly sensitive assay. The nanoscale-gap electrode amplifies the amperometric signals from p-aminophenol liberated from artificial substrates in the final steps of the cascade reactions. First, the cross reaction between the LAL and coagulation cascade reactions was investigated. The results indicated that these cascade reactions did not efficiently proceed in a single solution owing to the cross reaction. Therefore, we selected to induce two cascade reactions in different solutions by bisecting the beads after the immunocomplex formation on the beads. The cross reactions of factor XIa with the LAL cascade reaction and of endotoxin with the coagulation cascade reaction were investigated. The effects of these cross reactions were revealed to be negligible by bisecting the beads before inducing the cascade reactions. Finally, a dual immunoassay for goat and human immunoglobulin G was performed, for which the limits of detection were 70 pg/mL (470 fmol/L) and 1.0 ng/mL (6.6 pmol/L), respectively. Thus, our dual immunoassay provides a sensitive platform for clinical diagnosis requiring detection of multiple analytes.
Electrochemical sensing of oxygen metabolism for a three-dimensional cultured model with biomimetic vascular flow Peer-reviewed

Yuji Nashimoto, Rei Mukomoto, Takuto Imaizumi, Takato Terai, Shotaro Shishido, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Kunishige Onuma, Masahiro Inoue, Hitoshi Shiku

Biosensors and Bioelectronics　219　114808-114808　2022/10
Publisher: Elsevier BV
DOI： 10.1016/j.bios.2022.114808 　

ISSN： 0956-5663
Detection of virus-like particles using magnetostrictive vibration energy harvesting Peer-reviewed

Hiroki Kurita, Siti Masturah Binti Fakhruddin, Daiki Neyama, Kumi Y. Inoue, Tsuyoki Tayama, Daiki Chiba, Masahito Watanabe, Hitoshi Shiku, Fumio Narita

Sensors and Actuators A: Physical　345　113814-113814　2022/10
Publisher: Elsevier BV
DOI： 10.1016/j.sna.2022.113814 　

ISSN： 0924-4247
Highly Sensitive Electrochemical Immunoassay Using Signal Amplification of the Coagulation Cascade Peer-reviewed

Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku

Analytical Chemistry　94　(36)　12427-12434　2022/08/26
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.2c02241 　

ISSN： 0003-2700

eISSN： 1520-6882
Electrochemiluminescence Imaging Based on Bipolar Electrochemistry Using Commercially Available Anisotropic Conductive Films Peer-reviewed

Rise Akasaka, Kosuke Ino, Tomoki Iwama, Kumi Y. Inoue, Yuji Nashimoto, Hitoshi Shiku

Sensors and Materials　34　(8)　3113-3122　2022/08/15
Publisher: MYU K.K.
DOI： 10.18494/sam3745 　

ISSN： 0914-4935

eISSN： 2435-0869
Electrochemical microwell sensor with Fe–N co-doped carbon catalyst to monitor nitric oxide release from endothelial cell spheroids Peer-reviewed

Kaoru Hiramoto, Kazuyuki Iwase, Yoshinobu Utagawa, Yuji Nashimoto, Itaru Honma, Kosuke Ino, Hitoshi Shiku

Analytical Sciences　38　(10)　1297-1304　2022/07/27
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s44211-022-00160-0 　

ISSN： 0910-6340

eISSN： 1348-2246
Dissolved Oxygen-Sensing Chip Integrating an Open Container Connected with a Position-Raised Channel for Estimation of Cellular Mitochondrial Activity Peer-reviewed

Ching-Chou Wu, Chieh-Jen Wang, Cicero Lee-Tian Chang, Hitoshi Shiku, Yu-Ren Wang, Jia-De Yan, Shinn-Jyh Ding

ACS Sensors　7　(7)　1808-1818　2022/07/22
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acssensors.1c02287 　

ISSN： 2379-3694

eISSN： 2379-3694
Fabrication of High-Density Vertical Closed Bipolar Electrode Arrays by Carbon Paste Filling Method for Two-Dimensional Chemical Imaging International-journal Peer-reviewed

Tomoki Iwama, Kumi Y. Inoue, Hitoshi Shiku

Analytical Chemistry　94　(25)　8857-8866　2022/06/28
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.1c05354 　

ISSN： 0003-2700

eISSN： 1520-6882

More details Close

In this study, a carbon paste filling method was proposed as a simple strategy for fabricating high-density bipolar electrode (BPE) arrays for bipolar electrochemical microscopy (BEM). High spatiotemporal resolution imaging was achieved using the fabricated BPE array. BEM, which is an emerging microscopic system in recent years, achieves label-free and high spatiotemporal resolution imaging of molecular distributions using high-density BPE arrays and electrochemiluminescence (ECL) signals. We devised a simple method to fabricate a BPE array by filling a porous plate with carbon paste and succeeded in fabricating a high-density BPE array (15 μm pitch). After a detailed observation of the surface of the BPE array using a scanning electron microscope, the basic electrochemical and ECL emission characteristics were evaluated using potassium ferricyanide solution as a sample solution. Moreover, inflow imaging of the sample molecules was conducted to evaluate the imaging ability of the prepared BPE array. In addition, Prussian Blue containing carbon ink was applied to the sample solution side of the BPE array to provide catalytic activity to hydrogen peroxide, and the quantification and inflow imaging of hydrogen peroxide by ECL signals was achieved. This simple fabrication method of the BPE array can accelerate the research and development of BEM. Furthermore, hydrogen peroxide imaging by BEM is an important milestone for achieving bioimaging with high spatiotemporal resolution such as biomolecule imaging using enzymes.
Electrochemical glue for binding chitosan/alginate hydrogel fibers for cell culture International-journal Peer-reviewed

Yoshinobu Utagawa, Kosuke Ino, Tatsuki Kumagai, Kaoru Hiramoto, Masahiro Takinoue, Yuji Nashimoto, Hitoshi Shiku

Micromachines　13　(3)　420　2022/03

DOI： 10.3390/mi13030420 　
Electrochemiluminescence imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds International-journal Peer-reviewed

Kosuke Ino, Keika Komatsu, Kaoru Hiramoto, Yoshinobu Utagawa, Yuji Nashimoto, Hitoshi Shiku

Electrochimica Acta　415　140240　2022/03

DOI： 10.1016/j.electacta.2022.140240 　
Electrochemical Imaging of Endothelial Permeability Using a Large-Scale Integration-Based Device. International-journal Peer-reviewed

Kosuke Ino, Hao-Jen Pai, Kaoru Hiramoto, Yoshinobu Utagawa, Yuji Nashimoto, Hitoshi Shiku

ACS omega　6　(51)　35476-35483　2021/12/28

DOI： 10.1021/acsomega.1c04931 　

More details Close

It is important to clarify the transport of biomolecules and chemicals to tissues. Herein, we present an electrochemical imaging method for evaluating the endothelial permeability. In this method, the diffusion of electrochemical tracers, [Fe(CN)6]4-, through a monolayer of human umbilical vein endothelial cells (HUVECs) was monitored using a large-scale integration-based device containing 400 electrodes. In conventional tracer-based assays, tracers that diffuse through an HUVEC monolayer into another channel are detected. In contrast, the present method does not employ separated channels. In detail, a HUVEC monolayer is immersed in a solution containing [Fe(CN)6]4- on the device. As [Fe(CN)6]4- is oxidized and consumed at the packed electrodes, [Fe(CN)6]4- begins to diffuse through the monolayer from the bulk solution to the electrodes and the obtained currents depend on the endothelial permeability. As a proof-of-concept, the effects of histamine on the monolayer were monitored. Also, an HUVEC monolayer was cocultured with cancer spheroids, and the endothelial permeability was monitored to evaluate the metastasis of the cancer spheroids. Unlike conventional methods, the device can provide spatial information, allowing the interaction between the monolayer and the spheroids to be monitored. The developed method is a promising tool for organs-on-a-chip and drug screening in vitro.
Ion conductance-based perfusability assay of vascular vessel models in microfluidic devices International-journal Peer-reviewed

Rise Akasaka, Masashi Ozawa, Yuji Nashimoto, Kosuke Ino, Hitoshi Shiku

Micromachines　12　(12)　1491　2021/12

DOI： 10.3390/mi12121491 　
Thermally-Drawn Multi-Electrode Fibers for Bipolar Electrochemistry and Magnified Electrochemical Imaging Peer-reviewed

Tomoki Iwama, Yuanyuan Guo, Shoma Handa, Kumi Y. Inoue, Tatsuo Yoshinobu, Fabien Sorin, Hitoshi Shiku

ADVANCED MATERIALS TECHNOLOGIES　7　2101066　2021/11

DOI： 10.1002/admt.202101066 　

ISSN： 2365-709X
Electrochemical Immunoassay with Dual-Signal Amplification for Redox Cycling within a Nanoscale Gap Peer-reviewed

Kentaro Ito, Kumi Y. Inoue, Takahiro Ito-Sasaki, Kosuke Ino, Hitoshi Shiku

ACS Applied Nano Materials　4　12393-12400　2021/10/22
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acsanm.1c02837 　

ISSN： 2574-0970

eISSN： 2574-0970
Cover Feature: Detection and 2D Imaging of Dopamine Distribution Using a Closed Bipolar Electrode System by Applying a Cathodic Luminophore (ChemElectroChem 18/2021) Peer-reviewed

Tomoki Iwama, Mayo Komatsu, Kumi Y. Inoue, Hitoshi Shiku

ChemElectroChem　8　(18)　3428-3428　2021/09/13
Publisher: Wiley
DOI： 10.1002/celc.202101037 　
Detection and 2D Imaging of Dopamine Distribution Using a Closed Bipolar Electrode System by Applying a Cathodic Luminophore Peer-reviewed

Tomoki Iwama, Mayo Komatsu, Kumi Y. Inoue, Hitoshi Shiku

ChemElectroChem　8　(18)　3492-3498　2021/09/13
Publisher: Wiley
DOI： 10.1002/celc.202100675 　

ISSN： 2196-0216

eISSN： 2196-0216
Electrochemical Sensor to Detect Proteinuria Using Peptidases and Glutamate Oxidase Jointly Immobilized on a Prussian Blue-modified Electrode

Kentaro ITO, Kumi Y. INOUE, Tsubasa MIURA, Tomokazu MATSUE, Hitoshi SHIKU

Electrochemistry　89　(5)　409-414　2021/09/05

DOI： 10.5796/electrochemistry.21-00058 　

ISSN： 1344-3542

eISSN： 2186-2451
Topography and Permeability Analyses of Vasculature-on-a-Chip Using Scanning Probe Microscopies. International-journal Peer-reviewed

Yuji Nashimoto, Minori Abe, Ryota Fujii, Noriko Taira, Hiroki Ida, Yasufumi Takahashi, Kosuke Ino, Javier Ramon-Azcon, Hitoshi Shiku

Advanced healthcare materials　10　(21)　e2101186　2021/08/19

DOI： 10.1002/adhm.202101186 　

More details Close

Microphysiological systems (MPS) or organs-on-chips (OoC) can emulate the physiological functions of organs in vitro and are effective tools for determining human drug responses in preclinical studies. However, the analysis of MPS has relied heavily on optical tools, resulting in difficulties in real-time and high spatial resolution imaging of the target cell functions. In this study, the role of scanning probe microscopy (SPM) as an analytical tool for MPS is evaluated. An access hole is made in a typical MPS system with stacked microchannels to insert SPM probes into the system. For the first study, a simple vascular model composed of only endothelial cells is prepared for SPM analysis. Changes in permeability and local chemical flux are quantitatively evaluated during the construction of the vascular system. The morphological changes in the endothelial cells after flow stimulation are imaged at the single-cell level for topographical analysis. Finally, the possibility of adapting the permeability and topographical analysis using SPM for the intestinal vascular system is further evaluated. It is believed that this study will pave the way for an in situ permeability assay and structural analysis of MPS using SPM.
Electrochemiluminescence imaging of respiratory activity of cellular spheroids using sequential potential steps International-journal Peer-reviewed

Kaoru Hiramoto, Kosuke Ino, Keika Komatsu, Yuji Nashimoto, Hitoshi Shiku

Biosensors and Bioelectronics　181　113123-113123　2021/06
Publisher: Elsevier BV
DOI： 10.1016/j.bios.2021.113123 　

ISSN： 0956-5663

More details Close

The respiratory activity of cultured cells can be electrochemically monitored using scanning electrochemical microscopy (SECM) with high spatial resolution. However, in SECM, the electrode takes a long time to scan, limiting simultaneous measurements with large biological samples such as cell spheroids. Therefore, for rapid electrochemical imaging, a novel strategy is needed. Herein, we report electrochemiluminescence (ECL) imaging of spheroid respiratory activity for the first time using sequential potential steps. L-012, a luminol analog, was used as an ECL luminophore, and H2O2, a sensitizer for ECL of L-012, was generated by the electrochemical reduction of dissolved O2. The ECL imaging visualized spheroid respiratory activity-evidenced by ECL suppression-corresponding to O2 distribution around the spheroids. This method enabled the time-lapse imaging of respiratory activity in multiple spheroids with good spatial resolution comparable to that of SECM. Our work provides a promising high-throughput imaging strategy for elucidating spheroid cellular dynamics.
Nanoscale Visualization of Morphological Alteration of Live-Cell Membranes by the Interaction with Oligoarginine Cell-Penetrating Peptides Peer-reviewed

Hiroki Ida, Yasufumi Takahashi, Akichika Kumatani, Hitoshi Shiku, Tomo Murayama, Hisaaki Hirose, Shiroh Futaki, Tomokazu Matsue

Analytical Chemistry　93　5383-5393　2021/03/26
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.0c04097 　

ISSN： 0003-2700

eISSN： 1520-6882
Micropipet-Based Navigation in a Microvascular Model for Imaging Endothelial Cell Topography Using Scanning Ion Conductance Microscopy International-journal Peer-reviewed

Noriko Taira, Yuji Nashimoto, Kosuke Ino, Hiroki Ida, Takuto Imaizumi, Akichika Kumatani, Yasufumi Takahashi, Hitoshi Shiku

Analytical Chemistry　93　(11)　4902-4908　2021/03/23
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.analchem.0c05174 　

ISSN： 0003-2700

eISSN： 1520-6882

More details Close

Scanning ion conductance microscopy (SICM) has enabled cell surface topography at a high resolution with low invasiveness. However, SICM has not been applied to the observation of cell surfaces in hydrogels, which can serve as scaffolds for three-dimensional cell culture. In this study, we applied SICM for imaging a cell surface in a microvascular lumen reconstructed in a hydrogel. To achieve this goal, we developed a micropipet navigation technique using ionic current to detect the position of a microvascular lumen. Combining this navigation technique with SICM, endothelial cells in a microvascular model and blebs were visualized successfully at the single-cell level. To the best of our knowledge, this is the first report on visualizing cell surfaces in hydrogels using a SICM. This technique will be useful for furthering our understanding of the mechanism of intravascular diseases.
A Droplet Array Device for Electrochemical Detection of Methylene Blue Based on Local Redox Cycling Peer-reviewed

Kaoru HIRAMOTO, Keika KOMATSU, Yuta YAMADA, Yuji NASHIMOTO, Tomokazu MATSUE, Kosuke INO, Hitoshi SHIKU

BUNSEKI KAGAKU　70　(3)　183-189　2021/03/05
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.70.183 　

ISSN： 0525-1931
Electrodeposition of Thiolated Polymer-based Hydrogels via Disulfide Formation Using Electrogenerated Benzoquinone Peer-reviewed

Kosuke Ino, Ayako Tamura, Kaoru Hiramoto, Mika T. Fukuda, Yuji Nashimoto, Hitoshi Shiku

Chemistry Letters　50　(2)　256-259　2021/02/05

DOI： 10.1246/cl.200732 　

ISSN： 0366-7022

eISSN： 1348-0715
Microfluidic Separation of Blood Cells Based on the Negative Dielectrophoresis Operated by Three Dimensional Microband Electrodes Peer-reviewed

Tomoyuki Yasukawa, Junko Yamada, Hitoshi Shiku, Tomokazu Matsue, Masato Suzuki

Micromachines　11　(9)　833-833　2020/08/31
Publisher: MDPI AG
DOI： 10.3390/mi11090833 　

eISSN： 2072-666X
Electrochemical Bioimaging Platform Using Closed Bipolar Electrode Array

Kumi Y. Inoue, Tomoki Iwama, Siti Masturah Fakhruddin, Hitoshi Shiku, Tomokazu Matsue

ECS Meeting Abstracts　2020/05/01

DOI： 10.1149/MA2020-01332372mtgabs 　
Oxygen metabolism analysis of a vascularized spheroid using a scanning electrochemical microscopy

Yuji Nashimoto, Rei Mukomoto, Takato Terai, Kosuke Ino, Koichi Nishiyama, Ryuji Yokokawa, Takashi Miura, Hitoshi Shiku

MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences　520-521　2020
Publisher: Chemical and Biological Microsystems Society
LSI-based electrochemical device for evaluation of respiratory activity of three-dimensional cultured cells

Kosuke Ino, Takehiro Onodera, Hitoshi Shiku

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017　477-478　2020
Publisher: Chemical and Biological Microsystems Society
Scanning Electrochemical Cell Microscopy for Analysis of Solid Electrolyte Interface on Negative Electrodes in Lithium-Ion Batteries

Akichika Kumatani, Yuto Sato, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ECS Meeting Abstracts　{MA}2019-01　(47)　2267-2267　2019/05/01
Publisher: The Electrochemical Society
DOI： 10.1149/ma2019-01/47/2267 　

ISSN： 2151-2043

More details Close

<jats:p> Lithium-ion battery is one of the most popular secondary batteries for practical applications such as mobile phones and electrical cars because of their high energy density and good cyclability. For further improvement of their total performance with highly efficient cyclability, it is necessary to be in precise control of lithium-ion transport at the interface between electrolyte and electrodes. There is a major issue for efficient ion transport due to solid electrolyte interface (SEI) on negative composite electrodes such as graphite and/or silicon with some binders and electrical additives. The SEI is in general formed by decomposition of electrolyte. However, the SEI formation process or SEI itself is not still clear how the decomposition process proceeds due to the surface structure, binders or dispersion of active materials. Here, in this work we have used a scanning electrochemical microscopy (SECCM) with a single barrel nanopipette inside glove-box for analyzing local electrochemical reaction for SEI formation. A 50 nm radius nanopipette was filled with organic electrolyte (1.0 M LiPF<jats:sub>6</jats:sub> in ethylene carbonate:diethyl carbonate = 1:1 in vol. %) with a Li reference electrode. When the pipette was approached to the sample surface, a meniscus was created. Then, through the meniscus as a nanoscale electrochemical simulator, cyclic voltammogram was measured to introduce the SEI in localized area. The location SEI formed and their created potential were mapped by scanning the pipette on the sample. Further, on the particular structure such as basal and edge of graphite, the decomposition process was also analyzed for investigation of material structures for SEI formation. Those information would be particularly important to understand their mechanism and to control SEI formation. </jats:p>
Visualization of Inhomogeneuous Reactivity on Battery Material Using Scanning Electrochemical Cell Microscopy

Takahashi Yasufumi, Inomata Hirotaka, Daiko Takamatsu, Akichika Kumatani, Hitoshi Shiku, Tomokazu Matsue

ECS Meeting Abstracts　{MA}2019-01　(47)　2259-2259　2019/05/01
Publisher: The Electrochemical Society
DOI： 10.1149/ma2019-01/47/2259 　

ISSN： 2151-2043

More details Close

<jats:p> To understand the metal oxide coating effect on battery performance, the following two techniques are required: 1) constructing a flat thin-film electrode surface to realize a well-defined interface and 2) analyzing the electrode/electrolyte interface reaction with nanoscale resolution. We previously studied flat LiCoO<jats:sub>2</jats:sub> thin-film electrodes using<jats:italic> in situ</jats:italic> surface-sensitive X-ray absorption spectroscopy (XAS) and reported that Co reduction at the LiCoO<jats:sub>2</jats:sub> surface resulting from electrolyte contact caused the initial degradation. We also showed that the ZrO<jats:sub>2</jats:sub> layer successfully prevented physical contact between LiCoO<jats:sub>2</jats:sub> and the electrolyte. And it confirmed that a thicker ZrO<jats:sub>2</jats:sub> layer (above 2 nm) increased the diffusion resistance of the lithium ions in the ZrO<jats:sub>2</jats:sub> layer. However, since XAS lacks in-plane resolution and provides only averaged information, it is impossible to analyze the ZrO<jats:sub>2</jats:sub> morphology in detail. Recently, Taguchi et al. investigated a thin Li-Zr-layer (ca. 2 nm) on a LiCoO<jats:sub>2</jats:sub> composite electrode by transmission electron microscopy (TEM). They suggested that this thin layer could improve the durability.<jats:sup /> However, it is difficult to analyze the electrochemical properties using TEM. To evaluate the intrinsic mechanism of the metal oxide coating effect, it is necessary to develop a novel <jats:italic>in-situ</jats:italic> method that can analyze the surface morphology with high spatial resolution and simultaneously determine the local electrochemical properties. </jats:p> <jats:p>Scanning electrochemical microscopy (SECM) is a powerful technique for linking the surface morphology of a sample to its electrochemical properties. For the battery materials, the SECM feedback mode is effective in monitoring solid electrolyte interphase formation. To directly and quantitatively investigate spatially resolved ionic processes, mercury-capped platinum ultramicroelectrodes were developed and employed for Li<jats:sup>+</jats:sup> imaging based on Li stripping. However, it is difficult to visualize the Li<jats:sup>+</jats:sup> flux in battery materials at the sub-micrometer scale by SECM. Scanning electrochemical cell microscopy (SECCM), which uses a nanopipette as a probe and forms a local electrochemical cell, is effective in characterizing surface reactivity. We recently applied SECCM for visualization of electrochemical activities on a lithium-ion battery cathode material at sub-micrometer resolution. The SECCM was applied to collect or provide Li in specified area confined by the nanopipette. Further, it collection visualized the electrochemical properties by scanning the nanopipette as an image. There are some strong advantages in SECCM for battery material research such as its high spatial resolution, small capacitive current, and isolated electrochemical cell. </jats:p> <jats:p>In this report, we applied SECCM to characterize a ZrO<jats:sub>2</jats:sub>-coated LiCoO<jats:sub>2</jats:sub> thin-film electrode prepared by pulsed laser deposition. Local cyclic voltammetry (CV) and galvanostatic charge/discharge were performed to characterize the cycle durability and rate performance of ZrO<jats:sub>2</jats:sub>-coated LiCoO<jats:sub>2</jats:sub> thin-film electrodes and to reveal the relationship between the ZrO<jats:sub>2</jats:sub> morphology and thickness. </jats:p>
Electrochemical imaging using redox mediators for cell activity of three-dimensional cultured cells

Kosuke Ino, Takehiro Onodera, Mika T. Fukuda, Yuji Nashimoto, Hitoshi Shiku

Proceedings of SPIE - The International Society for Optical Engineering　11007　2019
Publisher: SPIE
DOI： 10.1117/12.2518825 　

ISSN： 1996-756X 0277-786X
Development of a high spatio-temporal resolution electrochemical imaging system using a closed bipolar electrode array

Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019　1380-1381　2019
Scanning electrochemical cell microscopy for visualization and local electrochemical activites of lithium-ion (de) intercalation process in lithium-ion batteryies electrodes. Peer-reviewed

A. Kumatani, Y. Takahashi, C. Miura, H. Ida, H. Inomata, H. Shiku, H. Munakata, K. Kanamura, T. Matsue

Surf. Interface Anal.　51　(1)　27-30　2019/01

DOI： 10.1002/sia.6538 　

ISSN： 0142-2421

eISSN： 1096-9918
Visualization of Inhomogeneous Current Distribution on ZrO2-Coated LiCoO2 Thin-Film Electrodes using Scanning Electrochemical Cell Microscopy. Peer-reviewed

H. Inomata, Y. Takahashi, D. Takamatsu, A. Kumatani, H. Shiku, T. Matsue

Chem Commun.　55　(4)　545-548　2019/01

DOI： 10.1039/c8cc08916g 　

ISSN： 1359-7345

eISSN： 1364-548X
Local hydrogel fabrication based on electrodeposition with a large-scale integration (LSI)-based amperometric device. Peer-reviewed

K. Ino, M. Terauchi, M. Gakumasawa, N. Taira, A. Suda, R. Kunikata, T. Matsue, H. Shiku

Sens. Actuat. B　277　95-101　2018/09

DOI： 10.1016/j.snb.2018.08.135 　
Simultaneous multiplex potentiostatic electroanalysis with liquid-junction-removed reference electrode system using a closed bipolar electrode. Peer-reviewed

K. Y. Inoue, M. Ikegawa, T. Ito-Sasaki, S. Takano, H. Shiku, T. Matsue

ChemElectroChem　5　(16)　2167-2170　2018/09

DOI： 10.1002/celc.201800536 　

ISSN： 2196-0216
Front Cover: Simultaneous Multiplex Potentiostatic Electroanalysis with Liquid-Junction-Removed Reference Electrode System using a Closed Bipolar Electrode (ChemElectroChem 16/2018)

Kumi Y. Inoue, Miho Ikegawa, Takahiro Ito-Sasaki, Shinichiro Takano, Hitoshi Shiku, Tomokazu Matsue

ChemElectroChem　5　(16)　2157-2157　2018/08/09
Publisher: Wiley
DOI： 10.1002/celc.201800895 　
Nanoscale Electrochemical Imaging of Aligned Semiconducting and Metallic Single-Walled Carbon Nanotube Bundles

Akichika Kumatani, Takeru Okada, Takahashi Yasufumi, Miyu Shimura, Hitoshi Shiku, Seiji Samukawa, Tomokazu Matsue

ECS Meeting Abstracts　{MA}2018-02　(9)　564-564　2018/07/23
Publisher: The Electrochemical Society
DOI： 10.1149/ma2018-02/9/564 　

ISSN： 2151-2043

More details Close

<jats:p> Carbon nanotubes have been studied in the variety of research fields from electronic devices to energy harvesting applications [1]. In general, their unique properties come from their ideal one-dimensional structure and tremendous metallic or semiconducting behavior depending upon their chirality. For energy application, it is an important issue to develop a good separation technique for the metallic and semiconducting single-walled carbon nanotubes (SWNTs) in large volume and to understand their electrochemical activities of both types of nanotubes. Recent progress for separation between metallic and semiconducting of has been developed. For example, a gel-based separation technique reported by Tanaka and co-workers [2]. Also, Unwin’s group has revealed the electrochemical activities for each type of the individual SWNT by scanning electrochemical cell microscopy [3]. </jats:p> <jats:p>In this study, we have at first created aligned SWNT bundles by dielectrophoresis [4] using by the commercially available suspension created by a gel separation technique. The aligned nanotube samples were prepared from either semiconducting or metallic nanotube suspension. Then, scanning electrochemical cell microscopy with a single-barrel nanopipette [5] was applied to each sample for the detection of the existence of the other property of the nanotube, referring to the difference in electrochemical activities between semiconducting and metallic nanotubes [3]. </jats:p> <jats:p>[1] M. F. L. De Volder et al., Science 339, 535, (2013). [2] T. Tanaka et al., Nano Lett. 9, 1497 (2009). [3] A. G. Guel et al., Nano Lett., 14, 220 (2014). [4] R. Krupke et al., Science 301, 344 (2003). [5] Y. Takahashi et al. Nat. Comm. 5:6450 (2014). </jats:p>
Micropatterning of nafion membranes on an electrode array using photolithographic and lift-off techniques for selective electrochemical detection and signal accumulation Peer-reviewed

Mayuko Terauchi, Kosuke Ino, Yusuke Kanno, Shunsuke Imai, Hitoshi Shiku, Tomokazu Matsue

Chemistry Letters　47　(2)　204-206　2018
Publisher: Chemical Society of Japan
DOI： 10.1246/cl.171031 　

ISSN： 1348-0715 0366-7022
Fabrication of poly(ethylene glycol) hydrogels containing vertically and horizontally aligned graphene using dielectrophoresis: An experimental and modeling study Peer-reviewed

Samad Ahadian, Ushio Naito, Velappa Jayaraman Surya, Sorour Darvishi, Mehdi Estili, Xiaobin Liang, Ken Nakajima, Hitoshi Shiku, Yoshiyuki Kawazoe, Tomokazu Matsue

CARBON　123　460-470　2017/10

DOI： 10.1016/j.carbon.2017.07.082 　

ISSN： 0008-6223

eISSN： 1873-3891
Redox Reaction on the Surface/Edge Strturtures of Graphene/Graphite

Akichika Kumatani, Chiho Miura, Hitoshi Shiku, Takahashi Yasufumi, Tomokazu Matsue

ECS Meeting Abstracts　{MA}2017-02　(8)　660-660　2017/09/01
Publisher: The Electrochemical Society
DOI： 10.1149/ma2017-02/8/660 　

ISSN： 2151-2043

More details Close

<jats:p> Graphene, a single atomic layer of graphite, has been widely studied because of its unique electrical, optical, electrochemical, and elastic properties. With respect to the electrochemical property, it is also expected for energy harvesting and storage applications such as hydrogen generation for fuel cells and ion transport for secondary batteries. Indeed, the graphene’s exotic phenomena come from its ideal structure such as edge states, two-dimensional surface, defects. To understand their intrinsic mechanism due to structure effects, it is required to analyze and visualize their electrochemical reaction by a spatially resolved electrochemical analysis technique at microscopic scale. In general, electrochemical analytical tools are conducted at mesoscopic scale. Scanning probe microscopies have been also utilized for analyzing their properties to satisfy the issue in resolution. Scanning tunneling microscopy and conductive atomic force microscopy can analyze at atomic scale precisely, but their information is mainly limited to electronic properties which do not directly reflect to their electrochemical properties. In this study, to overcome the issue, scanning electrochemical cell microscopy with sub-micrometer resolution, i.e. nanoSECCM, was utilized as a direct technique for electrochemical reactions [1]. A glass nano-pipette was applied as a probe. The pipettes were filled electrolyte and a reference electrode (5 mM hexaammineruthenium chloride and 25 mM KCl in phosphate buffer or deionized water for electrolyte, Ag/AgCl for a reference electrode). The nanoSECCM was applied to cleaved graphite or graphene on silicon substrates. When the pipette was approached to the sample surface, a meniscus was created as an electrochemical cell. Through the cell, redox reaction of ruthenium ion inside electrolyte was measured. By scanning the pipette with the same process at next measurement location, redox reaction on the graphene/graphite surface were visualized as shown in Fig 1. </jats:p> <jats:p>[1] Y. Takahashi, A. Kumatani <jats:italic>et al</jats:italic>. Nat. Comm. 5:6450 (2014).</jats:p> <jats:p> </jats:p> <jats:p> <jats:inline-formula> <jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="660fig1.jpeg" xlink:type="simple" /> </jats:inline-formula> </jats:p> <jats:p>Figure 1</jats:p> <jats:p />
Carbon nanotubes embedded in embryoid bodies direct cardiac differentiation Peer-reviewed

Samad Ahadian, Shukuyo Yamada, Mehdi Estili, Xiaobin Liang, Ramin Banan Sadeghian, Ken Nakajima, Hitoshi Shiku, Tomokazu Matsue, Ali Khademhosseini

BIOMEDICAL MICRODEVICES　19　(3)　57-57　2017/09

DOI： 10.1007/s10544-017-0184-1 　

ISSN： 1387-2176

eISSN： 1572-8781
Advanced Scanning Electrochemical Microscope System for High-Resolution imaging and Electrochemical Applications Peer-reviewed

Ryo Matsuoka, Shigeo Aoyagi, Naoshi Matsumoto, Masaaki Matsudaira, Yasufumi Takahashih, Akichika Kumatanid, Hiroki Ida, Hirokazu Munakata, Katsuhiko Iida, Hitoshi Shiku, Kiyoshi Kanamura, Tomokazu Matsue

ELECTROCHEMISTRY　85　(6)　319-326　2017/06

DOI： 10.5796/electrochemistry.85.319 　

ISSN： 1344-3542
Binary-number-based digital electrochemical detection using a single working electrode with multiple sensors Peer-reviewed

Kosuke Ino, Yusuke Kanno, Yuta Yamada, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY COMMUNICATIONS　77　76-80　2017/04

DOI： 10.1016/j.elecom.2017.02.016 　

ISSN： 1388-2481

eISSN： 1873-1902
Cell Sheet Fabrication Using RGD Peptide-coupled Alginate Hydrogels Fabricated by an Electrodeposition Method Peer-reviewed

Fumisato Ozawa, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

CHEMISTRY LETTERS　46　(4)　605-608　2017/04

DOI： 10.1246/cl.170003 　

ISSN： 0366-7022

eISSN： 1348-0715
Amperometric Detection of Apoptosis by using p-Methoxyaniline-Conjugated Substrate for Caspase-3 Peer-reviewed

Sun Sixiang, Kumi Y. Inoue, Shusaku Shiomoto, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

CHEMELECTROCHEM　4　(4)　941-946　2017/04

DOI： 10.1002/celc.201600700 　

ISSN： 2196-0216
Three-dimensional co-culture of C2C12/PC12 cells improves skeletal muscle tissue formation and function Peer-reviewed

Serge Ostrovidov, Samad Ahadian, Javier Ramon-Azcon, Vahid Hosseini, Toshinori Fujie, S. Prakash Parthiban, Hitoshi Shiku, Tomokazu Matsue, Hirokazu Kaji, Murugan Ramalingam, Hojae Bae, Ali Khademhosseini

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE　11　(2)　582-595　2017/02

DOI： 10.1002/term.1956 　

ISSN： 1932-6254

eISSN： 1932-7005
Molecular electrochemical switching element based on diffusive molecular competition for multipoint electrochemical detection of respiration activity of cell aggregates Peer-reviewed

Kosuke Ino, Yuta Yamada, Yusuke Kanno, Shunsuke Imai, Hitoshi Shiku, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　234　201-208　2016/10

DOI： 10.1016/j.snb.2016.04.160 　

ISSN： 0925-4005
Localized Electrochemical Analysis of Polyacrylate Binders on Graphite-Silicon Negative Electrodes in Lithium-Ion Batteries

Akichika Kumatani, Tetsuya Watanabe, Takahashi Yasufumi, Kei Kubota, Hiroki Ida, Hitoshi Shiku, Shinichi Komaba, Tomokazu Matsue

ECS Meeting Abstracts　{MA}2016-02　(3)　492-492　2016/09/01
Publisher: The Electrochemical Society
DOI： 10.1149/ma2016-02/3/492 　

ISSN： 2151-2043

More details Close

<jats:p>Graphite-silicon composite negative electrodes are expected to satisfy one of recent demands for creation of high performance lithium-ion batteries because of their high-capacity (above 1000 mAh/g). A major issue for their practical application is a control of large volume expansion/shrinkage of silicon materials, which is a key to realize their good reversible cyclability. Recently, Komaba <jats:italic>et al</jats:italic>. reported that polyacrylate binders improve their cyclability because of well-dispersion of graphite/silicon materials by coating them with polyacrylate homogeniously.<jats:sup>1,2</jats:sup> Also, polyacrylate can suppress a formation of decomposition of organic electrolyte during lithiation process, which induces poor cyclability. For further investigation of existence of the decomposition, we have applied a scanning electrochemical cell microscopy with single barrel nano-pipette filled with organic electrolyte and a reference electrode, called nanoSECCM in Figure 1 a.<jats:sup>3</jats:sup>NanoSECCM can measure electrochemical properties (such by cyclic voltammetry, CV) with sub-micrometer scale locally. From localized CV results (scan rate at 100 mV/s) as shown in Figure 1 b, we have observed current response related to the formation of decomposition. This response was not observed by typical bulk CV measurement, suggesting that our technique is sensitive to investigate graphite-silicon composite negative electrodes for realization of high performance electrodes in lithium-ion batteries. </jats:p> <jats:p>[1] N. Yabuuchi <jats:italic>et al</jats:italic>., <jats:italic>Adv. Energy Mater.</jats:italic>, 2011, 1, 759.</jats:p> <jats:p>[2] Z-J. Han <jats:italic>et al</jats:italic>., Phys. <jats:italic>Chem. Chem. Phys.</jats:italic>, 2015, 17, 3783.</jats:p> <jats:p>[3] Y. Takahashi <jats:italic>et al</jats:italic>., <jats:italic>Nat. Commu</jats:italic>n. 2014, 5, 6450.</jats:p> <jats:p /> <jats:p> <jats:inline-formula> <jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="492fig1.jpeg" xlink:type="simple" /> </jats:inline-formula> </jats:p> <jats:p>Figure 1</jats:p> <jats:p />
Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture Peer-reviewed

Fumisato Ozawa, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

MATERIALS　9　(9)　744-744　2016/09

DOI： 10.3390/ma9090744 　

ISSN： 1996-1944
Liquid-junction-free system for substitutional stripping voltammetry using a closed bipolar electrode system Peer-reviewed

Shinichiro Takano, Kumi Y. Inoue, Miho Ikegawa, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY COMMUNICATIONS　66　34-37　2016/05

DOI： 10.1016/j.elecom.2016.02.014 　

ISSN： 1388-2481

eISSN： 1873-1902
Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device Peer-reviewed

Hiroya Abe, Yusuke Kanno, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY　84　(5)　364-367　2016/05

DOI： 10.5796/electrochemistry.84.364 　

ISSN： 1344-3542
Redox Cycling-based Electrochemical Reporter Gene Assay for Single Cells Using a Scanning Electrochemical Microscope-microwell System Peer-reviewed

Hiroki Ida, Kosuke Ino, Junya Suzuki, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY　84　(5)　308-311　2016/05

DOI： 10.5796/electrochemistry.84.308 　

ISSN： 1344-3542
Potentiometric bioimaging with a large-scale integration (LSI)-based electrochemical device for detection of enzyme activity Peer-reviewed

Yusuke Kanno, Kosuke Ino, Chika Sakamoto, Kumi Y. Inoue, Masahki Matsudaira, Atsushi Suda, Ryota Kunikata, Tomohiro Ishikawa, Hiroya Abe, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　77　709-714　2016/03

DOI： 10.1016/j.bios.2015.10.021 　

ISSN： 0956-5663

eISSN： 1873-4235
Digital electrochemical array detection on a single working electrode

K. Ino, H. Shiku, T. Matsue

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016　1294-1295　2016
Publisher: Chemical and Biological Microsystems Society
Graphene induces spontaneous cardiac differentiation in embryoid bodies Peer-reviewed

Samad Ahadian, Yuanshu Zhou, Shukuyo Yamada, Mehdi Estili, Xiaobin Liang, Ken Nakajima, Hitoshi Shiku, Tomokazu Matsue

NANOSCALE　8　(13)　7075-7084　2016

DOI： 10.1039/c5nr07059g 　

ISSN： 2040-3364

eISSN： 2040-3372
Developing biomedical nano-grained beta-type titanium alloys using high pressure torsion for improved cell adherence Peer-reviewed

Hakan Yilmazer, Mustafa Sen, Mitsuo Niinomi, Masaaki Nakai, Liu Huihong, Ken Cho, Yoshikazu Todaka, Hitoshi Shiku, Tomokazu Matsue

RSC ADVANCES　6　(9)　7426-7430　2016

DOI： 10.1039/c5ra23454a 　

ISSN： 2046-2069

eISSN： 2046-2069
Local Redox-cycling-based Electrochemical System for Bioimaging Peer-reviewed

Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

BUNSEKI KAGAKU　64　(9)　669-678　2015/09

ISSN： 0525-1931
Microtubule guiding in a multi-walled carbon nanotube circuit Peer-reviewed

Aurélien Sikora, Javier Ramón-Azcón, Mustafa Sen, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Hitoshi Shiku, Tomokazu Matsue, Winfried Teizer

Biomedical Microdevices　17　(4)　2015/08
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s10544-015-9978-1 　

ISSN： 1387-2176

eISSN： 1572-8781
Microtubule guiding in a multi-walled carbon nanotube circuit. Peer-reviewed

Sikora A, Ramón-Azcón J, Sen M, Kim K, Nakazawa H, Umetsu M, Kumagai I, Shiku H, Matsue T, Teizer W

Biomedical microdevices　17　(4)　78　2015/08

DOI： 10.1007/s10544-015-9978-1 　

ISSN： 1387-2176

eISSN： 1572-8781
Current Response at Grain and Grain Boundaries in LiFePO₄ Thin Films By Nano-Scanning Electrochemical Cell Microscopy

Akichika Kumatani, Yasufumi Takahashi, Hirotaka Inomata, Tetsuya Watanabe, Kuniko Yamamoto, Masakazu Haruta, Susumu Shiraki, Hitoshi Shiku, Taro Hitosugi, Tomokazu Matsue

ECS Meeting Abstracts　{MA}2015-02　(1)　88-88　2015/07/07
Publisher: The Electrochemical Society
DOI： 10.1149/ma2015-02/1/88 　

ISSN： 2151-2043

More details Close

<jats:p>Creation of high performance electrodes in rechargeable lithium-ion batteries (LIBs) is one of urgent demand to store unused energy towards low-carbon emission society. To satisfy this demand, a facile characterization technique is required for analyzing electrochemical response related to lithium-ion transport via crystal orientation or crystal boundaries in the electrodes. Recently, we have developed a single-channel nano-scanning electrochemical call microscopy (nanoSECCM).<jats:sup>1</jats:sup> The nanoSECCM can obtain current response related to ion transport only as <jats:italic>in-situ </jats:italic>measurement with nanometer resolution. In this study, we have applied nanoSECCM to characterize the current repose at grain and grain boundaries on the LiFePO<jats:sub>4</jats:sub> thin film electrode. The film is multi-domain structure with sub-micrometer grains including [010] crystal orientation, confirmed by SEM and XRD analyses. The inhomogeneity of the response at grains was visualized from nanoSECCM mapping. The response at particular grain with higher response presumably indicates that it possesses [010] crystal orientation which is known as higher current response in LiFePO<jats:sub>4</jats:sub>.<jats:sup>2</jats:sup> We show that this analysis is a sensitive and facile measure of the influence for crystal orientation and boundaries in electrodes of LIBs. </jats:p> <jats:p>[1] Y. Takahashi, A. Kumatani <jats:italic>et al</jats:italic>., Nat. Communi. <jats:bold>5</jats:bold>:5450 (2014).</jats:p> <jats:p>[2] D. Morgan <jats:italic>et a</jats:italic>l., Electrochem. Solid State Lett. <jats:bold>7</jats:bold>, A30 (2004).</jats:p>
Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity Peer-reviewed

Yusuke Kanno, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL SCIENCES　31　(7)　715-719　2015/07

DOI： 10.2116/analsci.31.715 　

ISSN： 0910-6340

eISSN： 1348-2246
Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors Peer-reviewed

Hiroya Abe, Kosuke Ino, Chen-Zhong Li, Yusuke Kanno, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　87　(12)　6364-6370　2015/06

DOI： 10.1021/acs.analchem.5b01307 　

ISSN： 0003-2700

eISSN： 1520-6882
Feedback mode-based electrochemical imaging of conductivity and topography for large substrate surfaces using an LSI-based amperometric chip device with 400 sensors Peer-reviewed

Yusuke Kanno, Kosuke Ino, Kumi Y. Inoue, Mustafa Sen, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hiroya Abe, Chen-Zhong Li, Hitoshi Shiku, Tomokazu Matsue

JOURNAL OF ELECTROANALYTICAL CHEMISTRY　741　109-113　2015/03

DOI： 10.1016/j.jelechem.2015.01.020 　

ISSN： 1572-6657

eISSN： 1873-2569
Electrochemical imaging using molecular consumption-based switching system for evaluation of respiration activity of cell aggregates

K. Ino, Y. Yamada, H. Shiku, T. Matsue

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences　1698-1700　2015
Publisher: Chemical and Biological Microsystems Society
Mechanical properties and cytocompatibility of oxygen-modified beta-type Ti-Cr alloys for spinal fixation devices Peer-reviewed

Huihong Liu, Mitsuo Niinomi, Masaaki Nakai, Ken Cho, Kengo Narita, Mustafa Sen, Hitoshi Shiku, Tomokazu Matsue

ACTA BIOMATERIALIA　12　352-361　2015/01

DOI： 10.1016/j.actbio.2014.10.014 　

ISSN： 1742-7061

eISSN： 1878-7568
Facile and green production of aqueous graphene dispersions for biomedical applications Peer-reviewed

Samad Ahadian, Mehdi Estili, Velappa Jayaraman Surya, Javier Ramon-Azcon, Xiaobin Liang, Hitoshi Shiku, Murugan Ramalingam, Tomokazu Matsue, Yoshio Sakka, Hojae Bae, Ken Nakajima, Yoshiyuki Kawazoe, Ali Khademhosseini

NANOSCALE　7　(15)　6436-6443　2015

DOI： 10.1039/c4nr07569b 　

ISSN： 2040-3364

eISSN： 2040-3372
Hydrogels containing metallic glass sub-micron wires for regulating skeletal muscle cell behaviour Peer-reviewed

Samad Ahadian, Ramin Banan Sadeghian, Shin Yaginuma, Javier Ramon-Azcon, Yuji Nashimoto, Xiaobin Liang, Hojae Bae, Ken Nakajima, Hitoshi Shiku, Tomokazu Matsue, Koji S. Nakayama, Ali Khademhosseini

BIOMATERIALS SCIENCE　3　(11)　1449-1458　2015

DOI： 10.1039/c5bm00215j 　

ISSN： 2047-4830

eISSN： 2047-4849
A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies Peer-reviewed

Yusuke Kanno, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　15　(23)　4404-4414　2015

DOI： 10.1039/c5lc01016k 　

ISSN： 1473-0197

eISSN： 1473-0189
Facile and rapid generation of 3D chemical gradients within hydrogels for high-throughput drug screening applications Peer-reviewed

Samad Ahadian, Javier Ramon-Azcon, Mehdi Estili, Raquel Obregon, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　59　166-173　2014/09

DOI： 10.1016/j.bios.2014.03.031 　

ISSN： 0956-5663

eISSN： 1873-4235
Electrochemical monitoring of intracellular enzyme activity of single living mammalian cells by using a double-mediator system Peer-reviewed

Yoshiharu Matsumae, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICA CHIMICA ACTA　842　20-26　2014/09

DOI： 10.1016/j.aca.2014.06.047 　

ISSN： 0003-2670

eISSN： 1873-4324
Electrochemical evaluation of sarcomeric alpha-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array Peer-reviewed

Mustafa Sen, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL METHODS　6　(16)　6337-6342　2014/08

DOI： 10.1039/c4ay00791c 　

ISSN： 1759-9660

eISSN： 1759-9679
Nanoscale Cell Surface Topography Imaging using Scanning Ion Conductance Microscopy Peer-reviewed

Yasufumi Takahashi, Komachi Ito, Xiongwei Wang, Yoshiharu Matsumae, Hirokazu Komaki, Akichika Kumatani, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY　82　(5)　331-334　2014/05

DOI： 10.5796/electrochemistry.82.331 　

ISSN： 1344-3542
Electrochemical Approach for the Development of a Simple Method for Detecting Cell Apoptosis Based on Caspase-3 Activity Peer-reviewed

Shinichiro Takano, Shusaku Shiomoto, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　86　(10)　4723-4728　2014/05

DOI： 10.1021/ac403394z 　

ISSN： 0003-2700

eISSN： 1520-6882
Densified Electrochemical Sensors Based on Local Redox Cycling between Vertically Separated Electrodes in Substrate Generation/Chip Collection and Extended Feedback Modes Peer-reviewed

Kosuke Ino, Yusuke Kanno, Taku Nishijo, Hirokazu Komaki, Yuta Yamada, Shinya Yoshida, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　86　(8)　4016-4023　2014/04

DOI： 10.1021/ac500435d 　

ISSN： 0003-2700

eISSN： 1520-6882
SU-8-based flexible amperometric device with IDA electrodes to regenerate redox species in small spaces Peer-reviewed

Yusuke Kanno, Takehito Goto, Kosuke Ino, Kumi Y. Inoue, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Analytical Sciences　30　(2)　305-309　2014/02

DOI： 10.2116/analsci.30.305 　

ISSN： 0910-6340 1348-2246
Lsi-based amperometric chip device with 400 sensors for detection of alkaline phosphatase and respiration activities of embryonic stem cells

Y. Kanno, K. Ino, K. Y. Inoue, A. Suda, R. Kunikata, M. Matsudaira, H. Shiku, T. Matsue

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014　2152-2154　2014
Publisher: Chemical and Biological Microsystems Society
3P105 Real-time Monitoring of Membrane Receptor Internalization : Low-Invasive, Quantitative and Single-Cell Level Measurement by SECM(03. Membrane proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Matsumae Yoshiharu, Takahashi Yasufumi, Ino Kosuke, Shiku Hitoshi, Matsue Tomokazu

Seibutsu Butsuri　54　(1)　S266　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S266_3 　
1P292 Single-cell nanobiopsy to investigate intracellular mRNA localization using scanning ion conductance microscopy(26. Measurements,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Nashimoto Yuji, Takahashi Yasufumi, Ino Kosuke, Inoue Y Kumi, Shiku Hitoshi, Matsue Tomokazu

Seibutsu Butsuri　54　(1)　S189　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S189_4 　
2P303 Development of Nano Electrochemical Mircoscope for living cell imaging(27. Bioimaging,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Takahashi Yasufumi, Mustafa Sen, Matsumae Yoshiharu, Ino Kosuke, Shiku Hitoshi, Matsue Tomokazu

Seibutsu Butsuri　54　(1)　S245　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S245_3 　
（Secondary Publication）Tacrolimus inhibits the revascularization of isolated pancreatic islets Peer-reviewed

NISHIMURA Ryuichi, NISHIOKA Sho, FUJISAWA Ikuma, SHIKU Hitoshi, SHIMADA Miki, SEKIGUCHI Satoshi, FUJIMORI Keisei, USHIYAMA Akira, MATSUE Tomokazu, OHUCHI Noriaki, SATOMI Susumu, GOTO Masafumi

Japanese Journal of Transplantation　49　(4 & 5)　353-362　2014
Publisher: The Japan Society for Transplantation
DOI： 10.11386/jst.49.353 　

ISSN： 0578-7947

More details Close

【Objective】Immunosuppressive drugs could be crucial factors for a poor outcome after islet allotransplantation. Unlike rapamycin, the effects of tacrolimus, the current standard immunosuppressant used in islet transplantation, on graft revascularization remain unclear. We examined the effects of tacrolimus on islet revascularization using a highly sensitive imaging system, and analyzed the gene expression in transplanted islets by introducing laser microdissection techniques. 【Methods】Islets isolated from C57BL/6-Tg (CAG-EGFP) mice were transplanted into the nonmetallic dorsal skinfold chamber on the recipients. Balb/c athymic mice were used as recipients and divided into two groups, including a control group (n=9) and tacrolimus-treated group (n=7). Changes in the newly-formed vessels surrounding the islet grafts were imaged and semi-quantified using multi-photon laser-scanning microscopy and a Volocity system. Gene expression in transplanted islets was analyzed by the BioMark dynamic system. 【Results】The revascularization process was completed within 14 days after pancreatic islet transplantation at subcutaneous sites. The newly-formed vascular volume surrounding the transplanted islets in the tacrolimus-treated group was significantly less than that in the control group (p＜0.05). Although the expression of Vegfa (p＜0.05) and Ccnd1 (p＜0.05) was significantly upregulated in the tacrolimus-treated group compared with that of the control group, no differences were observed between the groups in terms of other types of gene expression. 【Conclusion】The present study demonstrates that tacrolimus inhibits the revascularization of isolated pancreatic islets without affecting the characteristics of the transplanted grafts. Further refinements of this immunosuppressive regimen, especially regarding the revascularization of islet grafts, could improve the outcome of islet allotransplantation. *This article is a secondary publication in Japanese of "Tacrolimus inhibits the revascularization of isolated pancreatic islets" published by Nishimura R, Nishioka S, Fujisawa I, et al. PLoS One.2013 Apr 17; 8. DOI: 10.1371/journal.pone.56799
Three-dimensional co-culture of C2C12/PC12 cells improves skeletal muscle tissue formation and function Peer-reviewed

Ostrovidov, Serge, Ahadian, Samad, Ramon-Azcon, Javier, Hosseini, Vahid, Fujie, Toshinori, Prakash Parthiban, Selvakumar, Shiku, Hitoshi, Matsue, Tomokazu, Kaji, Hirokazu, Ramalingam, Murgan, Bae, Hojae, Khademhosseini, Ali

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE　in press　2014

DOI： 10.1002/term.1956 　

ISSN： 1932-7005
Detection of the Oxygen Consumption Rate of Migrating Zebrafish by Electrochemical Equalization Systems Peer-reviewed

Tomoyuki Yasukawa, Masahiro Koide, Norihisa Tatarazako, Ryoko Abe, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue

ANALYTICAL CHEMISTRY　86　(1)　304-307　2014/01

DOI： 10.1021/ac402962f 　

ISSN： 0003-2700

eISSN： 1520-6882
Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device Peer-reviewed

Kosuke Ino, Takehito Goto, Yusuke Kanno, Kumi Y. Inoue, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　14　(4)　787-794　2014

DOI： 10.1039/c3lc51156a 　

ISSN： 1473-0197

eISSN： 1473-0189
Electrically regulated differentiation of skeletal muscle cells on ultrathin graphene-based films Peer-reviewed

Samad Ahadian, Javier Ramon-Azcon, Haixin Chang, Xiaobin Liang, Hirokazu Kaji, Hitoshi Shiku, Ken Nakajima, Murugan Ramalingam, Hongkai Wu, Tomokazu Matsue, Ali Khademhosseini

RSC ADVANCES　4　(19)　9534-9541　2014

DOI： 10.1039/c3ra46218h 　

ISSN： 2046-2069
Electrochemical sensor with substitutional stripping voltammetry for highly sensitive endotoxin assay Peer-reviewed

Shinichiro Takano, Kumi Y. Inoue, Satoko Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYST　139　(19)　5001-5006　2014

DOI： 10.1039/c4an00510d 　

ISSN： 0003-2654

eISSN： 1364-5528
Non-invasive measurement of glucose uptake of skeletal muscle tissue models using a glucose nanobiosensor Peer-reviewed

Raquel Obregon, Samad Ahadian, Javier Ramon-Azcon, Luyang Chen, Takeshi Fujita, Hitoshi Shiku, Mingwei Chen, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　50　194-201　2013/12

DOI： 10.1016/j.bios.2013.06.020 　

ISSN： 0956-5663
LSI-based amperometric sensor for real-time monitoring of embryoid bodies Peer-reviewed

Mustafa Sen, Kosuke Ino, Kumi Y. Inoue, Toshiharu Arai, Taku Nishijo, Atsushi Suda, Ryota Kunikata, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　48　12-18　2013/10

DOI： 10.1016/j.bios.2013.03.069 　

ISSN： 0956-5663

eISSN： 1873-4235
Electrochemical device with interdigitated ring array electrodes for investigating the relationship between cardiomyocyte differentiation from embryonic stem cells and alkaline phosphatase activity Peer-reviewed

Kosuke Ino, Taku Nishijo, Yusuke Kanno, Fumisato Ozawa, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Electrochemistry　81　(9)　682-687　2013/09

DOI： 10.5796/electrochemistry.81.682 　

ISSN： 1344-3542 2186-2451

eISSN： 2186-2451
Carbon-Ag/AgCl probes for detection of cell activity in droplets Peer-reviewed

Kosuke Ino, Kaoru Ono, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Analytical Chemistry　85　(8)　3832-3835　2013/04/16

DOI： 10.1021/ac303569t 　

ISSN： 0003-2700 1520-6882
Electrodeposition of alginate gels for construction of vascular-like structures Peer-reviewed

Fumisato Ozawa, Kosuke Ino, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Journal of Bioscience and Bioengineering　115　(4)　459-461　2013/04

DOI： 10.1016/j.jbiosc.2012.10.014 　

ISSN： 1389-1723 1347-4421

eISSN： 1347-4421
A contactless electrical stimulator: Application to fabricate functional skeletal muscle tissue Peer-reviewed

Samad Ahadian, Javier Ramón-Azcón, Serge Ostrovidov, Gulden Camci-Unal, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

Biomedical Microdevices　15　(1)　109-115　2013/02

DOI： 10.1007/s10544-012-9692-1 　

ISSN： 1387-2176 1572-8781
Densified electrochemical sensor based on vertically separated electrode array for electrochemical imaging

K. Ino, Y. Kanno, H. Komaki, H. Shiku, T. Matsue

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013　2　1282-1284　2013
Publisher: Chemical and Biological Microsystems Society
1P298 Mapping of neurotransmitter releasing sites using scanning electrochemical ion conductance microscopy(27. Bioimaging,Poster)

Takahashi Yasufumi, Wang Xiongwe, Ino Kosuke, Shiku Hitoshi, Matsue Tomoakazu

Seibutsu Butsuri　53　(1)　S155　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S155_3 　
1P265 Collection and quantification of messenger RNA from tissue models by double barrel carbon probe(21A. Genome biology: Genome analysis,Poster)

Nashimoto Yuji, Takahashi Yasufumi, Takano Ryosuke, Miyashita Kosuke, Yamada Shukuyo, Ino Kosuke, Shiku Hitoshi, Matsue Tomokazu

Seibutsu Butsuri　53　(1)　S149　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S149_6 　
抗体スポットを用いた表面抗原発現細胞の選択的補足 Peer-reviewed

安川智之, 山田純子, 珠玖仁, 水谷文雄, 末永智一

表面技術　(64)　52-56　2013

DOI： 10.4139/sfj.64.52 　
Alginate gel microwell arrays using electrodeposition for three-dimensional cell culture Peer-reviewed

Fumisato Ozawa, Kosuke Ino, Toshiharu Arai, Javier Ramon-Azcon, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　13　(15)　3128-3135　2013

DOI： 10.1039/c3lc50455g 　

ISSN： 1473-0197

eISSN： 1473-0189
Cell pairing using a dielectrophoresis-based device with interdigitated array electrodes Peer-reviewed

Mustafa Sen, Kosuke Ino, Javier Ramon-Azcon, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　13　(18)　3650-3652　2013

DOI： 10.1039/c3lc50561h 　

ISSN： 1473-0197

eISSN： 1473-0189
A screen-printed endotoxin sensor based on amperometry using a novel p-aminophenol conjugated substrate for a Limulus amebocyte lysate protease reaction Peer-reviewed

Kumi Y. Inoue, Shinichiro Takano, Satoko Takahashi, Yosuke Ishida, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ANALYST　138　(21)　6523-6531　2013

DOI： 10.1039/c3an01202f 　

ISSN： 0003-2654

eISSN： 1364-5528
Positioning of cells flowing in a fluidic channel by negative dielectrophoresis Peer-reviewed

Tomoyuki Yasukawa, Junko Yamada, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue

Sensors and Actuators, B: Chemical　186　9-16　2013

DOI： 10.1016/j.snb.2013.05.048 　

ISSN： 0925-4005
Patterning with particles using three-dimensional interdigitated array electrodes with negative dielectrophoresis and its application to simple immunosensing Peer-reviewed

Masashi Yamamoto, Tomoyuki Yasukawa, Masato Suzuki, Satoshi Kosuge, Hitoshi Shiku, Tomokazu Matsue, Fumio Mizutani

ELECTROCHIMICA ACTA　82　35-42　2012/11

DOI： 10.1016/j.electacta.2012.02.109 　

ISSN： 0013-4686

eISSN： 1873-3859
A Facile Method for Patterned Growth of ZnO Nanowires Using a Black Ink Peer-reviewed

Sang Hyun Lee, Hyun Jung Lee, Hitoshi Shiku, Takafumi Yao, Tomokazu Matsue

ELECTRONIC MATERIALS LETTERS　8　(5)　511-513　2012/10

DOI： 10.1007/s13391-012-2047-5 　

ISSN： 1738-8090

eISSN： 2093-6788
タクロリムスが移植膵島の血管新生に及ぼす影響

西村隆一, 西岡翔, 珠玖仁, 島田美樹, 関口悟, 藤盛啓成, 牛山明, 末永智一, 里見進, 後藤昌史

移植　47　(総会臨時)　235-235　2012/09
Publisher: (一社)日本移植学会
ISSN： 0578-7947

eISSN： 2188-0034
Microfluidic heavy metal immunoassay based on absorbance measurement. Peer-reviewed

Y. Date, S. Terakado, K. Sasaki, A. Aota, N. Matsumoto, H. Shiku, K. Ino, Y. Watanabe, T. Matsue, N. Ohmura

Biosens. Bioelectron.　33　(1)　106-112　2012/05/01

DOI： 10.1016/j.bios.2011.12.030. 　
Local redox cycling-based electrochemical chip devicefor high-throughput assay toward evaluating embryoid bodies

Kosuke Ino, Taku Nishijo, Yusuke Kanno, Hitoshi Shiku, Tomokazu Matsue

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012　1345-1347　2012
Publisher: Chemical and Biological Microsystems Society
Electrochemical detection of secreted alkaline phosphatase (SEAP) from transformed hela cells using a lab-on-a-chip device based on target concentration and local redox-cycling

Mustafa Şen, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012　1918-1920　2012
Publisher: Chemical and Biological Microsystems Society
An application of interdigitated array of Pt electrodes for electrical stimulation of engineered muscle tissue Peer-reviewed

Ahadian S, Ram{\'o}n-Azc{\'o}n J, Ostrovidov S, Kaji H, Shiku H, Khademhosseini A, Matsue T

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012　1132-1134　2012
Rapid and Simple Immunoassay Based on Negative Dielectrophoresis with Three-Dimensional Interdigitated Array Electrodes Peer-reviewed

T. Yasukawa, H. Shiku, T. Matsue, F. Mizutani

CHEMICAL SENSORS 10 -AND- MEMS/NEMS 10　50　(12)　139-146　2012

DOI： 10.1149/05012.0139ecst 　

ISSN： 1938-5862
Improvement of Detectable Sensitivity for Enzyme Reaction by Scanning Electrochemical Microscopy with Distance Control System for Immunosensing Peer-reviewed

Yu Hirano, Tomoyuki Yasukawa, Yoshiaki Mase, Daisuke Oyamatsu, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue

ELECTROCHEMISTRY　80　(1)　30-32　2012/01

DOI： 10.5796/electrochemistry.80.30 　

ISSN： 1344-3542
Microfluidic devices for electrochemical measurement of photosynthetic activity of cyanobacteria Microcystis cells Peer-reviewed

Masahiro Koide, Tomoyuki Yasukawa, Yoshiko Horiguchi, Kuniaki Nagamine, Hitoshi Shiku, Tomokazu Matsue, Tomoaki Itayama

Analytical Sciences　28　(1)　69-72　2012

DOI： 10.2116/analsci.28.69 　

ISSN： 0910-6340 1348-2246

eISSN： 1348-2246
Interdigitated array of Pt electrodes for electrical stimulation and engineering of aligned muscle tissue Peer-reviewed

Samad Ahadian, Javier Ramon-Azcon, Serge Ostrovidov, Gulden Camci-Unal, Vahid Hosseini, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

LAB ON A CHIP　12　(18)　3491-3503　2012

DOI： 10.1039/c2lc40479f 　

ISSN： 1473-0197

eISSN： 1473-0189
Electrochemical detection for dynamic analyses of a redox component in droplets using a local redox cycling-based electrochemical (LRC-EC) chip device Peer-reviewed

Kosuke Ino, Yusuke Kanno, Taku Nishijo, Takehito Goto, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

CHEMICAL COMMUNICATIONS　48　(68)　8505-8507　2012

DOI： 10.1039/c2cc34264b 　

ISSN： 1359-7345
Accumulation and detection of secreted proteins from single cells for reporter gene assays using a local redox cycling-based electrochemical (LRC-EC) chip device Peer-reviewed

Mustafa Sen, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　12　(21)　4328-4335　2012

DOI： 10.1039/c2lc40674h 　

ISSN： 1473-0197
Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device Peer-reviewed

Xi Zhu, Kosuke Ino, Zhenyu Lin, Hitoshi Shiku, Guonan Chen, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　160　(1)　923-928　2011/12

DOI： 10.1016/j.snb.2011.09.004 　

ISSN： 0925-4005
Influence of Tip Size on Single Yeast Cell Imaging Using Scanning Electrochemical Microscopy Peer-reviewed

Kuniaki Nagamine, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ELECTROANALYSIS　23　(5)　1168-1174　2011/05

DOI： 10.1002/elan.201000595 　

ISSN： 1040-0397

eISSN： 1521-4109
An electrochemical device with microwells for determining the photosynthetic activity of a single cyanobacterium Peer-reviewed

Masahiro Koide, Tomoyuki Yasukawa, Kuniaki Nagamine, Hitoshi Shiku, Tomoaki Itayama, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　153　(2)　474-478　2011/04

DOI： 10.1016/j.snb.2010.10.051 　

ISSN： 0925-4005
Electrorotation chip consisting of three-dimensional interdigitated array electrodes Peer-reviewed

Kosuke Ino, Atsuko Ishida, Kumi Y. Inoue, Masato Suzuki, Masahiro Koide, Tomoyuki Yasukawa, Hitoshi Shikua, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　153　(2)　468-473　2011/04

DOI： 10.1016/j.snb.2010.11.012 　

ISSN： 0925-4005
Addressable electrode array device incorporated with IDA electrodes for biological analyses

K. Ino, T. Nishijo, W. Saito, H. Shiku, T. Matsue

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011　2　1278-1280　2011
Addressable electrode array device with IDA electrodes for high-throughput detection Peer-reviewed

Kosuke Ino, Wataru Saito, Masahiro Koide, Taizo Umemura, Hitoshi Shiku, Tomokazu Matsue

LAB ON A CHIP　11　(3)　385-388　2011

DOI： 10.1039/c0lc00437e 　

ISSN： 1473-0197
Fabrication of Line and Grid Patterns with Cells Based on Negative Dielectrophoresis. Peer-reviewed

T. Yasukawa, M. Suzuki, H. Shiku, T. Matsue

Journal of Robotics and Mechatronics　22　(5)　613-618　2010/10
Electrochemical detection of endotoxin using recombinant factor C zymogen Peer-reviewed

Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY COMMUNICATIONS　12　(8)　1066-1069　2010/08

DOI： 10.1016/j.elecom.2010.05.028 　

ISSN： 1388-2481
Rapid and simple immunosensing system for simultaneous detection of tumor markers based on negative-dielectrophoretic manipulation of microparticles Peer-reviewed

Hyun Jung Lee, Sang Hyun Lee, Tomoyuki Yasukawa, Javier Ramon-Azcon, Fumio Mizutani, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

TALANTA　81　(1-2)　657-663　2010/04

DOI： 10.1016/j.talanta.2009.12.058 　

ISSN： 0039-9140

eISSN： 1873-3573
Electrochemical monitoring of hydrogen peroxide released from leucocytes on horseradish peroxidase redox polymer coated electrode chip Peer-reviewed

Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku, Shigenobu Kasai, Tomoyuki Yasukawa, Fumio Mizutani, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　25　(7)　1723-1728　2010/03

DOI： 10.1016/j.bios.2009.12.014 　

ISSN： 0956-5663

eISSN： 1873-4235
Preparation of Immunosensors Using a Microfluidic Device with an Interdigitated Array Electrode Modified with Antibodies Invited Peer-reviewed

Yu Hirano, Tomoyuki Yasukawa, Yoshihiro Sawayashiki, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue

ELECTROCHEMISTRY　78　(2)　175-177　2010/02

DOI： 10.5796/electrochemistry.78.175 　

ISSN： 1344-3542
A multi-point detection system with addressable electrode array device incorporated with IDA electrodes

K. Ino, W. Saito, M. Koide, T. Umemura, H. Shiku, T. Matsue

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010　1　593-595　2010
Addressable electrochemiluminescence detection system based on redox-cycling of Ru(bpy)(3)(2+) Peer-reviewed

Zhenyu Lin, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue, Guonan Chen

CHEMICAL COMMUNICATIONS　46　(2)　243-245　2010

DOI： 10.1039/b915871e 　

ISSN： 1359-7345
Electrochemical topography of a cell monolayer with an addressable microelectrode array Peer-reviewed

Zhenyu Lin, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

CHEMICAL COMMUNICATIONS　46　(4)　559-561　2010

DOI： 10.1039/b915212a 　

ISSN： 1359-7345
Manipulation of Microparticles for Construction of Array Patterns by Negative Dielectrophoresis Using Multilayered Array and Grid Electrodes Peer-reviewed

Kosuke Ino, Hitoshi Shiku, Fumisato Ozawa, Tomoyuki Yasukawa, Tomokazu Matsue

BIOTECHNOLOGY AND BIOENGINEERING　104　(4)　709-718　2009/11

DOI： 10.1002/bit.22445 　

ISSN： 0006-3592

eISSN： 1097-0290
Microfluid-Assisted Dielectrophoretic Alignment and Device Characterization of Single ZnO Wires Peer-reviewed

Sang Hyun Lee, Hyun Jung Lee, Kosuke Ino, Hitoshi Shiku, Takafumi Yao, Tomokazu Matsue

JOURNAL OF PHYSICAL CHEMISTRY C　113　(45)　19376-19381　2009/11

DOI： 10.1021/jp908161v 　

ISSN： 1932-7447
Control of the microparticle position in the channel based on dielectrophoresis Peer-reviewed

Tomoyuki Yasukawa, Masato Suzuki, Hitoshi Shiku, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　142　(1)　400-403　2009/10

DOI： 10.1016/j.snb.2009.07.024 　

ISSN： 0925-4005
Detection of hormone active chemicals using genetically engineered yeast cells and microfluidic devices with interdigitated array electrodes Invited Peer-reviewed

Kosuke Ino, Yusuke Kitagawa, Tsuyoshi Watanabe, Hitoshi Shiku, Masahiro Koide, Tomoaki Itayama, Tomoyuki Yasukawa, Tomokazu Matsue

ELECTROPHORESIS　30　(19)　3406-3412　2009/10

DOI： 10.1002/elps.200900244 　

ISSN： 0173-0835
Three dimensional microelectrode array device integrating multi-channel microfluidics to realize manipulation and characterization of enzyme-immobilized polystyrene beads Peer-reviewed

Ryouta Kunikata, Yasufumi Takahashi, Masahiro Koide, Tomoaki Itayama, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　141　(1)　256-262　2009/08

DOI： 10.1016/j.snb.2009.05.028 　

ISSN： 0925-4005
Lateral arrays of vertical ZnO nanowalls on a periodically polarity-inverted ZnO template Peer-reviewed

Sang Hyun Lee, Tsutomu Minegishi, Jun-Seok Ha, Jin-sub Park, Hyo-Jong Lee, Hyun Jung Lee, Hitoshi Shiku, Tomokazu Matsue, Soon-Ku Hong, Heonsu Jeon, Takafumi Yao

NANOTECHNOLOGY　20　(23)　235304　2009/06

DOI： 10.1088/0957-4484/20/23/235304 　

ISSN： 0957-4484

eISSN： 1361-6528
Simple and rapid preparation of vertically aligned gold nanoparticle arrays and fused nanorods in pores of alumina membrane based on positive dielectrophoresis Peer-reviewed

Hyun Jung Lee, Tomoyuki Yasukawa, Masato Suzuki, Sang Hyun Lee, Takafumi Yao, Yusuke Taki, Akira Tanaka, Masaomi Kameyama, Hitoshi Shiku, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　136　(2)　320-325　2009/03

DOI： 10.1016/j.snb.2008.12.054 　

ISSN： 0925-4005
Detection of pesticide residues using an immunodevice based on negative dielectrophoresis Peer-reviewed

Javier Ramon-Azcon, Ryouta Kunikata, F. -J. Sanchez, M. -P. Marco, Hitoshi Shiku, Tomoyuki Yasukawa, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　24　(6)　1592-1597　2009/02

DOI： 10.1016/j.bios.2008.08.035 　

ISSN： 0956-5663
A microfludic device with interdigitated array electrodes for detection of hormone active chemicals using genetically-engineered yeast cells

Kosuke Ino, Yusuke Kitagawa, Hitoshi Shiku, Masahiro Koide, Yoshiko Horiguchi, Tomoaki Itayama, Tomoyuki Yasukawa, Tomokazu Matsue

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences　1623-1625　2009
Rapid immunosensing based on accumulation of microparticles by negative dielectrophoresis

Tomoyuki Yasukawa, Hyun Jung Lee, Javier Ramon-Azcon, Yusuke Yoshida, Hitoshi Shiku, Tomokazu Matsue, Fumio Mizutani

20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science　385-390　2009

DOI： 10.1109/MHS.2009.5352000 　
Entrapment and measurement of a biologically functionalized microbead with a microwell electrode Peer-reviewed

Ching-Yu Chang, Yasufumi Takahashi, Tatsuya Murata, Hitoshi Shiku, Hsien-Chang Chang, Tomokazu Matsue

LAB ON A CHIP　9　(9)　1185-1192　2009

DOI： 10.1039/b817705h 　

ISSN： 1473-0197
Negative dielectrophoretic patterning with different cell types Peer-reviewed

Masato Suzuki, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　24　(4)　1043-1047　2008/12

DOI： 10.1016/j.bios.2008.06.051 　

ISSN： 0956-5663
Rapid and separation-free sandwich immunosensing based on accumulation of microbeads by negative-dielectrophoresis Peer-reviewed

Hyun Jung Lee, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　24　(4)　1000-1005　2008/12

DOI： 10.1016/j.bios.2008.08.002 　

ISSN： 0956-5663
Cell-based electrochemical assay for endotoxin using a secreted alkaline phosphatase reporter system Peer-reviewed

Kumi Y. Inoue, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

ELECTROCHEMISTRY　76　(8)　525-528　2008/08

DOI： 10.5796/electrochemistry.76.525 　

ISSN： 1344-3542
Rapid fabrication of nanoparticles array on polycarbonate membrane based on positive dielectrophoresis Peer-reviewed

Hyun Jung Lee, Tomoyuki Yasukawa, Masato Suzuki, Yusuke Taki, Akira Tanaka, Masaomi Kameyama, Hitoshi Shiku, Tomokazu Matsue

SENSORS AND ACTUATORS B-CHEMICAL　131　(2)　424-431　2008/05

DOI： 10.1016/j.snb.2007.11.051 　

ISSN： 0925-4005
Electrophoretic cell manipulation and electrochemical gene-function analysis based on a yeast two-hybrid system in a microfluidic device Peer-reviewed

Tomoyuki Yasukawa, Kuniaki Nagamine, Yoshiko Horiguchi, Hitoshi Shiku, Masahiro Koide, Tomoaki Itayama, Fujio Shiraishi, Tomokazu Matsue

ANALYTICAL CHEMISTRY　80　(10)　3722-3727　2008/05

DOI： 10.1021/ac800143t 　

ISSN： 0003-2700

eISSN： 1520-6882
Whole-Cell Biosensors

H. Shiku, K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue

Bioelectrochemistry: Fundamentals, Experimental Techniques and Applications　249-266　2008/04/11
Publisher: John Wiley & Sons, Ltd
DOI： 10.1002/9780470753842.ch7 　
立体格子電極ウェルアレイデバイスによる微粒子の捕捉と電気化学検出

梅村太三, 珠玖仁, 高橋康史, 伊野浩介, 末永智一

化学工学会研究発表講演要旨集　2008　950-950　2008
Publisher: 公益社団法人化学工学会
DOI： 10.11491/scej.2008f.0.950.0 　
Rapid and Separation-Free Sandwich Immunosensing Based on Accmulation of Microbeads by Negative Dielectrophoresis Peer-reviewed

H.L.Lee, T.Yasukawa, H.Shiku, T. Matsue

Sensors and Actuators B　131　421-431　2008
Microcontact printed diaphorase monolayer on glass characterized by atomic force microscopy and scanning electrochemical microscopy Peer-reviewed

Hong Qun Luo, Hitoshi Shiku, Ayako Kumagai, Yasufumi Takahashi, Tomoyuki Yasukawa, Tomokazu Matsue

ELECTROCHEMISTRY COMMUNICATIONS　9　(11)　2703-2708　2007/11

DOI： 10.1016/j.elecom.2007.08.024 　

ISSN： 1388-2481
A competitive immunochromatographic assay for testosterone based on electrochemical detection Peer-reviewed

Kumi Inoue, Pascal Ferrante, Yu Hirano, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

TALANTA　73　(5)　886-892　2007/10

DOI： 10.1016/j.talanta.2007.05.008 　

ISSN： 0039-9140
Measurement of gene expression from single adherent cells and spheroids collected using fast electrical lysis Peer-reviewed

Yuji Nashimoto, Yasufumi Takahashi, Takeshi Yamakawa, Yu-Suke Torisawa, Tomoyuki Yasukawa, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Hitoshi Shiku, Hideki Kambara, Tomokazu Matsue

ANALYTICAL CHEMISTRY　79　(17)　6823-6830　2007/09

DOI： 10.1021/ac071050q 　

ISSN： 0003-2700

eISSN： 1520-6882
Enzyme immunosensing of pepsinogens 1 and 2 by scanning electrochemical microscopy Peer-reviewed

Tomoyuki Yasukawa, Yu Hirano, Naomi Motochi, Hitoshi Shiku, Tomokazu Matsue

BIOSENSORS & BIOELECTRONICS　22　(12)　3099-3104　2007/06

DOI： 10.1016/j.bios.2007.01.015 　

ISSN： 0956-5663

eISSN： 1873-4235
Electrochemical ELISA of testosterone using nitrocellulose membrane as a support for antibodies Peer-reviewed

Kumi Inoue, Pascal Ferrante, Yu Hirano, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

BUNSEKI KAGAKU　56　(6)　471-478　2007/06

DOI： 10.2116/bunsekikagaku.56.471 　

ISSN： 0525-1931
Flow sandwich-type immunoassay in microfluidic devices based on negative dielectrophoresis Peer-reviewed

Tomoyuki Yasukawa, Masato Suzuki, Takashi Sekiya, Hitoshi Shiku, Tomokazu Matsue

Biosensors and Bioelectronics　22　(11)　2730-2736　2007/05/15

DOI： 10.1016/j.bios.2006.11.010 　

ISSN： 0956-5663
Negative dielectrophoretic patterning with colloidal particles and encapsulation into a hydrogel Peer-reviewed

Masato Suzuki, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

Langmuir　23　(7)　4088-4094　2007/03/27

DOI： 10.1021/la063075a 　

ISSN： 0743-7463
Enzyme immunosensing for C-reactivc protein with scanning electrochemical microscopy Peer-reviewed

Tomoyuki Yasukawa, Yu Hirano, Daichi Ogasawara, Naomi Motochi, Hitoshi Shiku, Shohei Kawabata, Tomokazu Matsue

BUNSEKI KAGAKU　55　(12)　979-985　2006/12

DOI： 10.2116/bunsekikagaku.55.979 　

ISSN： 0525-1931
Topographic, electrochemical, and optical images captured using standing approach mode scanning electrochemical/optical microscopy Peer-reviewed

Yasufumi Takahashi, Yu Hirano, Tomoyuki Yasukawa, Hitoshi Shiku, Hiroshi Yamada, Tomokazu Matsue

LANGMUIR　22　(25)　10299-10306　2006/12

DOI： 10.1021/la0611763 　

ISSN： 0743-7463
Quality evaluation of in vitro-produced bovine embryos by respiration measurement and development of semi-automatic instrument Peer-reviewed

Shigeo Aoyagi, Yousuke Utsumi, Masaaki Matsudaira, Hiroshi Yamada, Masayuki Kaachi, Hitoshi Shiku, Hiroyuki Abe, Hiroyoshi Hoshi, Tomokazu Matsue

BUNSEKI KAGAKU　55　(11)　847-854　2006/11

DOI： 10.2116/bunsekikagaku.55.847 　

ISSN： 0525-1931
Cytokine assay on a cellular chip by combining collagen gel embedded culture with scanning electrochemical microscopy Peer-reviewed

S Kasai, H Shiku, YS Torisawa, K Nagamine, T Yasukawa, T Watanabe, T Matsue

ANALYTICA CHIMICA ACTA　566　(1)　55-59　2006/04

DOI： 10.1016/j.aca.2006.02.061 　

ISSN： 0003-2670
Electrochemical microdevice with separable electrode and antibody chips for simultaneous detection of pepsinogens 1 and 2 Peer-reviewed

D Ogasawara, Y Hirano, T Yasukawa, H Shiku, K Kobori, K Ushizawa, S Kawabata, T Matsue

BIOSENSORS & BIOELECTRONICS　21　(9)　1784-1790　2006/03

DOI： 10.1016/j.bios.2005.09.009 　

ISSN： 0956-5663
Development of negative dielectrophoretic cellular patterning system for co-culturing of two different cell types

Masato Suzuki, Tomoyuki Yasukawa, Mariko Komabayashi, Akik Inagaki, Yoshio Hori, Hitoshi Shiku, Tomokazu Matsue

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences　942-944　2006
Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device

Kuniaki Nagamine, Tomoyuki Yasukawa, Hitoshi Shiku, Masahiro Koide, Tomosato Itayama, Tomokazu Matsue

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences　645-647　2006
Electrochemical mutagen screening using microbial chip Peer-reviewed

N Matsui, T Kaya, K Nagamine, T Yasukawa, H Shiku, T Matsue

BIOSENSORS & BIOELECTRONICS　21　(7)　1202-1209　2006/01

DOI： 10.1016/j.bios.2005.05.004 　

ISSN： 0956-5663
Separation of live and dead microorganisms in a micro-fluidic device by dielectrophoresis Peer-reviewed

M Suzuki, T Yasukawa, H Shiku, T Matsue

BUNSEKI KAGAKU　54　(12)　1189-1195　2005/12

DOI： 10.2116/bunsekikagaku.54.1189 　

ISSN： 0525-1931
Functionalization of electrode surfaces with thionine modified Au nano-particles and its applications to electrochemical glucose sensors Invited Peer-reviewed

T. Yasukawa, M. K. Islam, T. Ohasi, M. Suto, H. Shiku, T. Matsue

Journal of the Surface Finishing Society of Japan　56　(10)　60-65　2005/10/01
Publisher: The Surface Finishing Society of Japan
DOI： 10.4139/sfj.56.616 　

ISSN： 0915-1869

More details Close

The electrochemically effective oxidation of NADH has drawn striking attention because it is widely applicable for fabricating biosensors. The mediators are normally used as electron shuttle molecules between the electrode and enzymes. Phenothiazine compounds deserve as suitable mediators due to theirs negative formal potential and large rate constant. For the practical use, both the enzyme and mediator are required to be co-immobilized on the electrode surface. In this study, gold nano-particles modified with thionine (Th+) were immobilized on the surface of the gold electrode by physical adsorption. Th+ was covalently bound to the surface of the gold nano-particles using 3,3'-dithiobis (succinimidyl propionate) (DSP) as a cross linker. The electrochemistry of Th+ modified on the gold nano-particles was characterized by voltammetry. Th+ molecules on the gold nano-particle modified electrodes showed two redox couples at −30mV and 240mV vs Ag/AgCl at pH7. A well-defined peak for the electrocatalytic oxidation of NADH was observed at 240mV. The nano-particle electrodes modified with glucose dehydrogenase (GDH) and Th+ mediators were fabricated and characterized aiming at fabricating a reagentless glucose biosensor. Upon the addition of glucose to the electrochemical cell, the oxidation current dramatically increased due to an electrocatalytic reaction.
Application of microbial chip for amperometric detection of metabolic alteration in bacteria Peer-reviewed

K Nagamine, T Kaya, T Yasukawa, H Shiku, T Matsue

SENSORS AND ACTUATORS B-CHEMICAL　108　(1-2)　676-682　2005/07

DOI： 10.1016/j.snb.2004.10.050 　

ISSN： 0925-4005
Amperometric detection of the bacterial metabolic regulation with a microbial array chip Peer-reviewed

K Nagamine, N Matsui, T Kaya, T Yasukawa, H Shiku, T Nakayama, T Nishino, T Matsue

BIOSENSORS & BIOELECTRONICS　21　(1)　145-151　2005/07

DOI： 10.1016/j.bios.2004.08.039 　

ISSN： 0956-5663
Dielectrophoretic micropatterning with microparticle monolayers covalently linked to glass surfaces Peer-reviewed

M Suzuki, T Yasukawa, Y Mase, D Oyamatsu, H Shiku, T Matsue

LANGMUIR　20　(25)　11005-11011　2004/12

DOI： 10.1021/la048111p 　

ISSN： 0743-7463
Scanning electrochemical microscopy for protein chip Imaging and shear force feedback regulation of substrate-probe distance Peer-reviewed

Y Hirano, D Oyamatsu, T Yasukawa, H Shiku, T Matsue

ELECTROCHEMISTRY　72　(2)　137-142　2004/02

ISSN： 1344-3542
Electrochemical/photochemical formation of enzyme patterns on glass substrates using a scanning electrochemical/confocal microscope Peer-reviewed

D Oyamatsu, N Kanaya, H Shiku, M Nishizawa, T Matsue

SENSORS AND ACTUATORS B-CHEMICAL　91　(1-3)　199-204　2003/06

DOI： 10.1016/S0925-4005(03)00089-3 　

ISSN： 0925-4005
Simultaneous detection of uric acid and glucose on a dual-enzyme chip using scanning electrochemical microscopy/scanning chemiluminescence microscopy Peer-reviewed

Shigenobu Kasai, Yu Hirano, Naomi Motochi, Hitoshi Shiku, Matsuhiko Nishizawa, Tomokazu Matsue

Analytica Chimica Acta　458　(2)　263-270　2002/05/06

DOI： 10.1016/S0003-2670(02)00064-8 　

ISSN： 0003-2670
Scanning electrochemical microscopy: SECM Peer-reviewed

H Shiku, H Ohya, T Matsuei

ELECTROCHEMISTRY　69　(10)　806-810　2001/10

ISSN： 1344-3542
Mapping peroxidase in plant tissues by scanning electrochemical microscopy Peer-reviewed

Huafang Zhou, Hitoshi Shiku, Shigenobu Kasai, Hiroyuki Noda, Tomokazu Matsue, Hiroaki Ohya-Nishiguchi, Hitoshi Kamada

Bioelectrochemistry　54　(2)　151-156　2001

DOI： 10.1016/S1567-5394(01)00123-2 　

ISSN： 1567-5394
Microvoltammetric characterization of diaphorase monolayer at glass surfaces Peer-reviewed

Hiroshi Yamada, Hitoshi Shiku, Tomokazu Matsue, Isamu Uchida

Bioelectrochemistry and Bioenergetics　33　(1)　91-93　1994

DOI： 10.1016/0302-4598(94)87038-1 　

ISSN： 0302-4598
Determination of active diaphorase immobilized at electrode surfaces Invited Peer-reviewed

Hiroshi Yamada, Hitoshi Shiku, Tomokazu Matsue, Isamu Uchida

Bioelectrochemistry and Bioenergetics　29　(3)　337-346　1993

DOI： 10.1016/0302-4598(93)85008-H 　

ISSN： 0302-4598
Electron transfer at a planar bilayer lipid membrane incorporated with 7,7,8,8-tetracyanoquinodimethane studied by ac impedance spectroscopy Peer-reviewed

Hiroshi Yamada, Hitoshi Shiku, Tomokazu Matsue, Isamu Uchida

Journal of Physical Chemistry　97　(38)　9547-9549　1993

DOI： 10.1021/j100140a001 　

ISSN： 0022-3654

Show all ︎Show first 5

Misc. 206

Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis

Kaoru Hiramoto, Elena Villani, Tomoki Iwama, Keika Komatsu, Shinsuke Inagi, Kumi Inoue, Yuji Nashimoto, Kosuke Ino, Hitoshi Shiku

Micromachines　11　(5)　530-530　2020/05/22
Publisher: MDPI AG
DOI： 10.3390/mi11050530 　

eISSN： 2072-666X

More details Close

Mammalian cell analysis is essential in the context of both fundamental studies and clinical applications. Among the various techniques available for cell analysis, electrochemiluminescence (ECL) has attracted significant attention due to its integration of both electrochemical and spectroscopic methods. In this review, we summarize recent advances in the ECL-based systems developed for mammalian cell analysis. The review begins with a summary of the developments in luminophores that opened the door to ECL applications for biological samples. Secondly, ECL-based imaging systems are introduced as an emerging technique to visualize single-cell morphologies and intracellular molecules. In the subsequent section, the ECL sensors developed in the past decade are summarized, the use of which made the highly sensitive detection of cell-derived molecules possible. Although ECL immunoassays are well developed in terms of commercial use, the sensing of biomolecules at a single-cell level remains a challenge. Emphasis is therefore placed on ECL sensors that directly detect cellular molecules from small portions of cells or even single cells. Finally, the development of bipolar electrode devices for ECL cell assays is introduced. To conclude, the direction of research in this field and its application prospects are described.
Biofabrication Using Electrochemical Devices and Systems Peer-reviewed

Kosuke Ino, Fumisato Ozawa, Ning Dang, Kaoru Hiramoto, Shodai Hino, Rise Akasaka, Yuji Nashimoto, Hitoshi Shiku

Advanced Biosystems　4　(4)　1900234-1900234　2020/04

DOI： 10.1002/adbi.201900234 　

ISSN： 2366-7478

eISSN： 2366-7478
電気化学計測デバイスを用いた細胞の消費物・分泌物評価 Invited

平本薫, 伊野浩介, 梨本裕司, 珠玖仁

Electrochemistry　87　323-328　2019/12

DOI： 10.5796/denkikagaku.19-FE0032 　
走査型イオンコンダクタンス顕微鏡による多項目ナノイメージング Invited

梨本裕司, 平典子, 伊野浩介, 珠玖仁

高分子　68　658-659　2019/12
Electric and Electrochemical Microfluidic Devices for Cell Analysis. International-journal Invited Peer-reviewed

K. Hiramoto, K. Ino, Y. Nashimoto, K. Ito, H. Shiku

Frontiers in Chemistry　7　396-396(7 pages)　2019/06

DOI： 10.3389/fchem.2019.00396 　

More details Close

Microfluidic devices are widely used for cell analysis, including applications for single-cell analysis, healthcare, environmental monitoring, and organs-on-a-chip that mimic organs in microfluidics. Moreover, to enable high-throughput cell analysis, real-time monitoring, and non-invasive cell assays, electric and electrochemical systems have been incorporated into microfluidic devices. In this mini-review, we summarize recent advances in these systems, with applications from single cells to three-dimensional cultured cells and organs-on-a-chip. First, we summarize microfluidic devices combined with dielectrophoresis, electrophoresis, and electrowetting-on-a-dielectric for cell manipulation. Next, we review electric and electrochemical assays of cells to determine chemical section activity, and oxygen and glucose consumption activity, among other applications. In addition, we discuss recent devices designed for the electric and electrochemical collection of cell components from cells. Finally, we highlight the future directions of research in this field and their application prospects.
Electrochemical biosensing system for single cells, cellular aggregates and microenvironments Invited Peer-reviewed

SHIKU Hitoshi

Anal. Sci.　35　(1)　29-38　2019/01

DOI： 10.2116/analsci.18SDR01 　
Characterization System of Embryos, Embryoid Bodies, and Multicellular Spheroids Based on an Electrochemical Method. Invited Peer-reviewed

SHIKU Hitoshi

Bunseki Kagaku　67　(11)　653-660　2018/11

DOI： 10.2116/bunsekikagaku.67.653 　
Intracellular Electrochemical Sensing Peer-reviewed

K. Ino∗, Y. Nashimoto, N. Taira, J. Ramon Azcon, H. Shiku∗

Electroanalysis　30　2195-2209　2018/10

DOI： 10.1002/elan.201800410 　
Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells Invited Peer-reviewed

Kosuke Ino, Mustafa Sen, Hitoshi Shiku, Tomokazu Matsue

ANALYST　142　(23)　4343-4354　2017/12

DOI： 10.1039/c7an01442b 　

ISSN： 0003-2654

eISSN： 1364-5528
Scanning Probe Microscopy for Nanoscale Electrochemical Imaging Invited Peer-reviewed

Yasufumi Takahashi, Akichika Kumatani, Hitoshi Shiku, Tomokazu Matsue

ANALYTICAL CHEMISTRY　89　(1)　342-357　2017/01

DOI： 10.1021/acs.analchem.6b04355 　

ISSN： 0003-2700

eISSN： 1520-6882
Estimation of respiratory activity of individual human embryos by scanning electrochemical microscopy Peer-reviewed

H. Abe, M. Yokoo, T. Itoh-Sasaki, M. Nasu, K. Goto, Y. Kumasako, Y. Araki, H. Shiku, T. Matsue, T. Utsunomiya

PROCEEDINGS OF THE 14TH WORLD CONGRESS ON IN VITRO FERTILIZATION & 3RD WORLD CONGRESS ON IN VITRO MATURATION　171-+　2007
Near-field scanning - Optical microscopy

H Shiku, RC Dunn

ANALYTICAL CHEMISTRY　71　(1)　23A-29A　1999/01

DOI： 10.1021/ac980576v 　

ISSN： 0003-2700
Recent advances in electrochemiluminescence sensing for in vitro cell analysis: a review

Kosuke Ino, Tomas Mockaitis, Ryota Shikuwa, Kimiharu Oba, Kaoru Hiramoto, Inga Morkvenaite-Vilkonciene, Hiroya Abe, Hitoshi Shiku

Analytical Sciences　2025

DOI： 10.1007/s44211-025-00723-x 　

ISSN： 0910-6340

eISSN： 1348-2246
生体模倣システムによる膵島の血管形成能力と遺伝子発現の照合

梨本裕司, 今野杏, 西川香里, 伊野浩介, 堀武志, 梶弘和, 新宅博文, 新宅博文, 後藤昌史, 珠玖仁

膵臓(Web)　39　(3)　2024

ISSN： 1881-2805
Electrochemical imaging for cell analysis in microphysiological systems International-journal Invited Peer-reviewed

An Konno, Kosuke Ino, Yoshinobu Utagawa, Hitoshi Shiku

Current Opinion in Electrochemistry　39　101270　2023/06

DOI： 10.1016/j.coelec.2023.101270 　
マイクロ流体デバイスを用いた単一膵島の血管形成能力の評価

梨本裕司, 今野杏, 西川香里, 伊野浩介, 堀武志, 梶弘和, 新宅博文, 新宅博文, 後藤昌史, 珠玖仁

日本分析化学会年会講演要旨集(Web)　72nd　2023
Bipolar Electrode Systems for Biosensing Peer-reviewed

Hitoshi Shiku

Review of Polarography　68　(2)　87-95　2022/10/21
Publisher: The Polarographic Society of Japan
DOI： 10.5189/revpolarography.68.87 　

ISSN： 0034-6691

eISSN： 1884-7692
Electrochemical Substrates and Systems for Enzyme-Based Bioassays Peer-reviewed

Yoshinobu UTAGAWA, Kentaro ITO, Kumi Y. INOUE, Yuji NASHIMOTO, Kosuke INO, Hitoshi SHIKU

BUNSEKI KAGAKU　71　(3)　109-117　2022/03/05
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.71.109 　

ISSN： 0525-1931
Bipolar Electrode‐based Electrochromic Devices for Analytical Applications – a Review Peer-reviewed

Siti Masturah binti Fakhruddin, Kosuke Ino, Kumi Y. Inoue, Yuji Nashimoto, Hitoshi Shiku

Electroanalysis　34　(2)　212-226　2022

DOI： 10.1002/elan.202100153 　

ISSN： 1040-0397

eISSN： 1521-4109
電気化学反応を使ったハイドロゲルのバイオファブリケーション

伊野浩介, 宇田川喜信, 梨本裕司, 珠玖仁

月刊バイオインダストリー　40-48　2021/10
In vitro electrochemical assays for vascular cells and organs International-journal Invited Peer-reviewed

Yoshinobu Utagawa, Kaoru Hiramoto, Yuji Nashimoto, Kosuke Ino, Hitoshi Shiku

Electrochemical Science Advances　e2100089　2021/10

DOI： 10.1002/elsa.202100089 　
(Invited) Closed Bipolar Electrochemistry for Sensor and Bioimaging Applications

Kumi Y. Inoue, Tomoki Iwama, Siti Masturah Fakhruddin, Hitoshi Shiku, Tomokazu Matsue

ECS Meeting Abstracts　2020/11/23

DOI： 10.1149/MA2020-02442811mtgabs 　
ヤヌス粒子を用いたアルドステロン症等の診断システムの開発

藪浩, 前田郁麻, 岩本恭典, 佐藤さつき, 井上久美, 珠玖仁, 末永智一, 根本靖久, 佐藤文俊

日本内分泌学会雑誌　95　(1)　2019

ISSN： 0029-0661
第3章細胞培養に関連した技術研究、第3節細胞計測技術 Invited

梨本裕司, 伊野浩介, 平典子, 珠玖仁

三次元培養における培養手法と周辺技術動向　83-97　2019
Simultaneous Multiplex Potentiostatic Electroanalysis with Liquid-Junction-Removed Reference Electrode System using a Closed Bipolar Electrode

Kumi Y. Inoue, Miho Ikegawa, Takahiro Ito-Sasaki, Shinichiro Takano, Hitoshi Shiku, Tomokazu Matsue

CHEMELECTROCHEM　5　(16)　2161-2161　2018/08

DOI： 10.1002/celc.201800894 　

ISSN： 2196-0216
酸化還元反応の差異による半導体・金属カーボンナノチューブの検出

熊谷明哉, 熊谷明哉, 志村実優, 高橋康史, 高橋康史, 三浦千穂, 岡田健, 岡田健, 井田大貴, 珠玖仁, 寒川誠二, 寒川誠二, 末永智一

応用物理学会春季学術講演会講演予稿集(CD-ROM)　65th　ROMBUNNO.17p‐P5‐4　2018/03/05
電気化学イメージングによる金属/半導体カーボンナノチューブの酸化還元反応の検証

志村実優, 熊谷明哉, 熊谷明哉, 岡田健, 三浦千穂, 井田大貴, 寒川誠二, 寒川誠二, 珠玖仁, 高橋康史, 高橋康史, 末永智一

電気化学会大会講演要旨集(CD-ROM)　85th　ROMBUNNO.1J12　2018/02/23
原発性アルドステロン症検査のための微小粒子を用いたイムノアッセイの検討

木内亮汰, 佐藤さつき, 伊藤健太郎, 井上久美, 藪浩, 伊野浩介, 末永智一, 珠玖仁

日本分析化学会年会講演要旨集　67th　2018
Bioelectrochemical applications of microelectrode arrays in cell analysis and engineering Invited Peer-reviewed

Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Current Opinion in Electrochemistry　5　(1)　146-151　2017/10/01
Publisher: Elsevier B.V.
DOI： 10.1016/j.coelec.2017.08.004 　

ISSN： 2451-9111 2451-9103
An approach to the research on ion and water properties in the interphase between the plasma membrane and bulk extracellular solution Invited Peer-reviewed

Hiroshi Hibino, Madoka Takai, Hidenori Noguchi, Seishiro Sawamura, Yasufumi Takahashi, Hideki Sakai, Hitoshi Shiku

JOURNAL OF PHYSIOLOGICAL SCIENCES　67　(4)　439-445　2017/07

DOI： 10.1007/s12576-017-0530-3 　

ISSN： 1880-6546

eISSN： 1880-6562
ALIGNMENT OF NANO CARBON MATERIALS USING DIELECTROPHORESIS AND APPLICATION FOR ELECTRODE ARRAY DEVICE

Proceedings of the Chemical Sensor Symposium　61　85-87　2017/03
Publisher: 電気化学会化学センサ研究会
抗原修飾ヤヌス粒子を用いたイムノアッセイ法の開発

佐藤(増子)さつき, 井上(安田)久美, 伊藤健太郎, FAKHRUDDIN Siti Masturah, 廣瀬理美, 珠玖仁, 珠玖仁, 佐藤文俊, 藪浩, 末永智一

日本分析化学会年会講演要旨集　66th　2017
DNAチップの開発に向けた電気化学クリック反応による表面修飾 Peer-reviewed

坂本ちか, 阿部博弥, 伊野浩介, 藤野智子, 磯部寛之, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム学会誌　16　(1)　41-42　2017
ナノ電気化学顕微鏡を用いた電極表面の局所電気化学測定. Invited Peer-reviewed

熊谷明哉, 高橋康史, 三浦千穂, 渡邊徹弥, 猪又宏貴, 棟方裕一, 珠玖仁, 金村聖志, 末永智一

表面科学　37　(10)　494-498　2016/10
Publisher: Surface Science Society Japan
DOI： 10.1380/jsssj.37.494 　

ISSN： 0388-5321

eISSN： 1881-4743
Carbon nanotubes and graphene-based nanomaterials for stem cell differentiation and tissue regeneration Peer-reviewed

Samad Ahadian, Raquel Obregón, Javier Ramón-Azcón, Georgina Salazar, Hitoshi Shiku, Murugan Ramalingam, Tomokazu Matsue

Journal of Nanoscience and Nanotechnology　16　(9)　8862-8880　2016/09/01
Publisher: American Scientific Publishers
DOI： 10.1166/jnn.2016.12729 　

ISSN： 1533-4899 1533-4880
Mechanical and Surface Functionalities of Nanostructured β-Type Titanium Alloys Through Severe Plastic Deformation Peer-reviewed

Hakan Yilmazer, Mtsuo Niinomi, Ken Cho, Masaaki Nakai, Huihoung Lui, Yoshikazu Todaka, Burak Dikici, Mustafa Şen, Hitoshi Shiku, Tomokazu Matsue

Proceedings of the 13th World Conference on Titanium　1761-1766　2016/05/06
Publisher: John Wiley & Sons, Inc.
DOI： 10.1002/9781119296126.ch296 　
DEVELOPMENT OF BIO-LSI WITH HIGH SPATIAL RESOLUTION FOR ESTIMATION OF CELL RESPIRATORY ACTIVITY

Proceedings of the Chemical Sensor Symposium　60　63-65　2016/03
Publisher: 電気化学会化学センサ研究会
DEVELOPMENT OF NOVEL DETECTION SYSTEM USING BIPOLAR ELECTRODE

Proceedings of the Chemical Sensor Symposium　60　78-80　2016/03
Publisher: 電気化学会化学センサ研究会
Multicolor electrochemical imaging for simultaneous multiplex cell assay using a chip device

Y. Kanno, K. Ino, H. Abe, K. Y. Inoue, M. Matsudaira, A. Suda, R. Kunikata, H. Shiku, T. Matsue

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016　104-105　2016
Local redox-cycling-based electrochemical system for bioimaging

Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Bunseki Kagaku　64　(9)　669-678　2015/10/06
Publisher: Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.64.669 　

ISSN： 0525-1931
高速走査型イオンコンダクタンス顕微鏡による細胞表面の微小構造動態の非侵襲測定

井田大貴, 高橋康史, 珠玖仁, 末永智一

第1回細胞生物若手の会　2015/06
生細胞表面のナノスケールの形状観察を実現する走査型イオンコンダクタンス顕微鏡の開発. Invited Peer-reviewed

井田大貴, 高橋康史, 珠玖仁, 末永智一

表面科学　36　(6)　313-318　2015/06
Publisher: The Surface Science Society of Japan
DOI： 10.1380/jsssj.36.313 　

ISSN： 0388-5321

More details Close

Live cell imaging is important to understand the cell function such as membrane dynamics. Scanning probe microscopy (SPM) is used for evaluation of cell surface topography with nano-scale, but in most cases the measurement induced cell damage when probe contact with the cell surface. Scanning Ion Conductance Microscopy (SICM) uses ion current as a feedback signal for nanopipette probe-sample distance control. SICM allows non-contact live cell imaging and high-resolution characterization of dynamic changes of cell surface. Furthermore, SICM can combine with other analytical tool as distance control technique.
エネルギーデバイスの最前線電気化学セル顕微鏡を用いた電池材料表面における充放電特性のナノスケール画像化技術 Peer-reviewed

高橋康史, 熊谷明哉, 猪又宏貴, 珠玖仁, 末永智一

エネルギーデバイス　2　(4)　66-69　2015/04
Publisher: 技術情報協会 = Technical Information Institute Co., Ltd.
ISSN： 2188-1383
走査型イオンコンダクタンス顕微鏡を用いたセネッセンス細胞の表面形状測定

井田大貴, 高橋康史, 松前義治, 伊野浩介, 珠玖仁, 末永智一

電気化学会大会講演要旨集(CD-ROM)　82nd　ROMBUNNO.3M02　2015/03/09
CMOS型電気化学デバイスを用いた神経細胞塊から放出される神経伝達物質のイメージング

阿部博弥, 伊野浩介, LI Chen‐Zhong, 菅野祐介, 井上久美, 國方亮太, 須田篤史, 松平昌昭, 高橋康史, 珠玖仁, 末永智一

電気化学会大会講演要旨集(CD-ROM)　82nd　ROMBUNNO.PS31　2015/03/09
マウス胚様体血管新生モデルにおける局所遺伝子解析

伊藤秀矩, 梨本裕司, 高橋康史, 伊野浩介, 珠玖仁, 末永智一

電気化学会大会講演要旨集(CD-ROM)　82nd　ROMBUNNO.1M02　2015/03/09
ナノ電気化学セル顕微鏡:有機ゲルピペットの電池材料評価への応用

熊谷明哉, 高橋康史, 猪又宏貴, 白木将, 山本邦子, 春田正和, 伊野浩介, 珠玖仁, 一杉太郎, 末永智一

電気化学会大会講演要旨集(CD-ROM)　82nd　ROMBUNNO.PBT03　2015/03/09
ナノ電気化学セル顕微鏡:有機ゲルピペットを用いた局所電気化学測定条件の検討

熊谷明哉, 高橋康史, 猪又宏貴, 白木将, 山本邦子, 春田正和, 伊野浩介, 珠玖仁, 一杉太郎, 末永智一

応用物理学会春季学術講演会講演予稿集(CD-ROM)　62nd　ROMBUNNO.14A-D9-4　2015/02/26
Osteoblast attachment on nanostructured β-type titanium alloys subjected to severe plastic deformation Peer-reviewed

H.Yilmazer, M.Niinomi, K.Cho, M.Nakai, H.Liu, Y.Todaka, D.Burak, M.Sen, H.Shiku, T.Matsue

日本歯科理工学会誌　2015
Lsi-based amperometric device for electrochemical imaging of drug effect on dopamine release from three-dimensional cultured pc12 cells Peer-reviewed

H. Abe, K. Ino, C. Z. Li, Y. Kanno, K. Y. Inoue, A. Suda, R. Kunikata, M. Matsudaira, Y. Takahashi, H. Shiku, T. Matsue

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences　1598-1600　2015
Potentiometric imaging of stem cells using an lsi-based electrochemical chip device with 400 microelectrodes

Y. Kanno, K. Ino, C. Sakamoto, K. Y. Inoue, M. Matsudaira, A. Suda, R. Kunikata, H. Abe, H. Shiku, T. Matsue

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences　1686-1688　2015
Scanning electrochemical microscopy for imaging single cells and biomolecules Peer-reviewed

Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Nanobiosensors and Nanobioanalyses　335-352　2015/01/01
Publisher: Springer Japan
DOI： 10.1007/978-4-431-55190-4_16 　
ナノスケール形状の非侵襲測定を可能にする高速走査型イオンコンダクタンス顕微鏡

井田大貴, 高橋康史, 高橋康史, 高橋康史, 珠玖仁, 末永智一, 末永智一

日本生化学会大会(Web)　88th　4T19L-08(3P0896) (WEB ONLY)　2015
LiFePO₄正極薄膜材料表面のイオン電流応答における結晶粒・粒界の影響

熊谷明哉, 高橋康史, 猪又宏貴, 白木将, 山本邦子, 春田正和, 伊野浩介, 珠玖仁, 一杉太郎, 末永智一

電池討論会講演要旨集　55th　252　2014/11/19
界面イオン伝導顕微鏡を利用した充放電特性の局所イメージング

高橋康史, 熊谷明哉, 猪俣宏貴, 棟方裕一, 伊野浩介, 珠玖仁, 金村聖志, 末永智一

電池討論会講演要旨集　55th　251　2014/11/19
Gradient Biomaterials as Tissue Scaffolds Peer-reviewed

Raquel Obregón, Javier Ramón-Azcón, Samad Ahadian, Hitoshi Shiku, Murugan Ramalingam, Ali Khademhosseini, Tomokazu Matsue

Stem Cell Biology and Tissue Engineering in Dental Sciences　175-186　2014/11/13
Publisher: Elsevier Inc.
DOI： 10.1016/B978-0-12-397157-9.00015-1 　
Dielectrophoretical fabrication of hybrid carbon nanotubes-hydrogel biomaterial for muscle tissue engineering applications Peer-reviewed

Javier Ramón-Azcón, Samad Ahadian, Raquel Obregon, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

Journal of Fluid Mechanics　1621　2014/11/06
Publisher: Cambridge University Press
DOI： 10.1557/opl.2014.70 　

ISSN： 1469-7645 0022-1120
Applications of Carbon Nanotubes in Stem Cell Research Peer-reviewed

Javier Ramon-Azcon, Samad Ahadian, Raquel Obregon, Hitoshi Shiku, Murugan Ramalingam, Tomokazu Matsue

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY　10　(10)　2539-2561　2014/10

DOI： 10.1166/jbn.2014.1899 　

ISSN： 1550-7033

eISSN： 1550-7041
マウスES細胞の神経細胞分化誘導系における呼吸活性と網羅的遺伝子解析

珠玖仁, 新井俊陽, ZHOU Y, 高橋康史, 伊野浩介, 井上久美, 末永智一

電気化学秋季大会講演要旨集　2014　202　2014/09/27
液絡構造を排した変換ストリッピングデバイスによる高感度エンドトキシン検出法の開発

高野真一朗, 井上(安田)久美, 高橋康史, 伊野浩介, 珠玖仁, 末永智一

電気化学秋季大会講演要旨集　2014　225　2014/09/27
界面イオン伝導顕微鏡を利用した充放電特性のリアルタイムイメージング

高橋康史, 熊谷明哉, 猪俣宏貴, 棟方裕一, 伊野浩介, 珠玖仁, 金村聖志, 末永智一

電気化学秋季大会講演要旨集　2014　245　2014/09/27
界面イオン伝導顕微鏡を用いたLiイオン電池電極表面におけるLi挿入脱離特性のマッピング

猪又宏貴, 熊谷明哉, 高橋康史, 棟方裕一, 珠玖仁, 金村聖志, 末永智一

化学系学協会東北大会プログラムおよび講演予稿集　2014　206　2014/09/20
走査型イオンコンダクタンス顕微鏡を用いたクラスリンピットの連続イメージング

井田大貴, 松前義治, 高橋康史, 伊野浩介, 珠玖仁, 末永智一

化学系学協会東北大会プログラムおよび講演予稿集　2014　91　2014/09/20
ES細胞を用いた血管新生モデルの構築と局所遺伝子解析

伊藤秀矩, 梨本裕司, 高橋康史, 伊野浩介, 珠玖仁, 末永智一

化学系学協会東北大会プログラムおよび講演予稿集　2014　90　2014/09/20
神経細胞から分泌されるシグナル物質の解析に向けた電気化学デバイスの開発

阿部博弥, 菅野祐介, 伊野浩介, 高橋康史, 井上(安田)久美, 珠玖仁, LI Chenzhong, 末永智一

化学系学協会東北大会プログラムおよび講演予稿集　2014　90　2014/09/20
走査型イオンコンダクタンス顕微鏡を用いた単一細胞ナノバイオプシー技術の開発と細胞内mRNA局在性の評価

梨本裕司, 高橋康史, 伊野浩介, 井上(安田)久美, 珠玖仁, 末永智一

日本分析化学会年会講演要旨集　63rd　26　2014/09/03
Improvement of LSI-based amperometric sensor array for wide application of bioimaging and biosensing Peer-reviewed

Kumi Y. Inoue, Masahki Matsudaira, Kosuke Ino, Masanori Nakano, Kosuke Takara, Atsushi Suda, Ryota Kunikata, Shinya Yoshida, Takeshi Hayasaka, Yoshiyuki Kikuchi, Xijiang Chang, Tomohiro Kubota, Hitoshi Shiku, Shuji Tanaka, Seiji Samukawa, Tomokaz

65th Annual Meeting of the International Society of Electrochemistry　2014/09
界面イオン伝導顕微鏡による正極薄膜材料表面におけるLiイオン挿入・脱離機構の可視化

熊谷明哉, 高橋康史, 猪又宏貴, 白木将, 山本邦子, 春田正和, 伊野浩介, 珠玖仁, 一杉太郎, 末永智一

応用物理学会秋季学術講演会講演予稿集(CD-ROM)　75th　ROMBUNNO.19A-A8-1　2014/09/01
細胞培養に向けたハイドロゲル電解析出法の開発

伊野浩介, 小沢文智, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム学会研究会講演要旨集　29th　31　2014/05/22
マイクロガラスピペットによるmicroRNA回収・分析法

宮下紘介, 梨本裕司, 高橋康史, 伊野浩介, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム学会研究会講演要旨集　29th　78　2014/05/22
微小ガラスプローブを用いたヒト培養細胞のmicroRNA回収および定量

宮下紘介, 梨本裕司, 高橋康史, 伊野浩介, 珠玖仁, 末永智一

分析化学討論会講演要旨集　74th　36　2014/05/10
電気化学的手法を用いた細胞活性評価

伊野浩介, 珠玖仁, 末永智一

生物工学会誌　92　(4)　176-179　2014/04
Dielectrophoretical fabrication of hybrid carbon nanotubes-hydrogel biomaterial for muscle tissue engineering applications Peer-reviewed

Javier Ramón-Azcón, Samad Ahadian, Raquel Obregon, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

Materials Research Society Symposium Proceedings　1621　2014
Publisher: Materials Research Society
DOI： 10.1557/opl.2014.70 　

ISSN： 0272-9172
Metallic Glass Nanofibers in Future Hydrogel-based Scaffolds Peer-reviewed

Ramin Banan Sadeghian, Samad Ahadian, Shin Yaginuma, Javier Ramon-Azcon, Xiaobin Liang, Ken Nakajima, Hitoshi Shiku, Tomokazu Matsue, Koji S. Nakayama, Ali Khademhosseini

2014 36TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC)　5276-5279　2014

ISSN： 1557-170X
1C11 Electrochemical lithography for fabricating alginate hydrogel

OZAWA Fumisato, INO Kosuke, SHIKU Hitoshi, MATSUE Tomokazu

The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME　2014　(0)　67-68　2014
Publisher: 一般社団法人日本機械学会
Electrodeposited alginate hydrogels for fabrication of cell sheets

K. Ino, F. Ozawa, H. Shiku, T. Matsue, T. Matsue

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014　509-511　2014/01/01
東北大学のMEMS拠点-異分野融合と協働から生まれたバイオLSI.

井上(安田)久美, 伊野浩介, 珠玖仁, 末永智一

Electrochemistry　82　(1)　37-42　2014/01
Publisher: The Electrochemical Society of Japan
DOI： 10.5796/electrochemistry.82.37 　

ISSN： 1344-3542
The Use of Microtechnology and Nanotechnology in Fabricating Vascularized Tissues

Raquel Obregon, Javier Ramon-Azcon, Samad Ahadian, Hitoshi Shiku, Hojae Bae, Murugan Ramalingam, Tomokazu Matsue

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY　14　(1)　487-500　2014/01

DOI： 10.1166/jnn.2014.9051 　

ISSN： 1533-4880

eISSN： 1533-4899
電気化学会創立80周年記念大会報告

末永智一, 珠玖仁, 伊藤隆

電気化学および工業物理化学 : denki kagaku　81　(7)　595-599　2013/07/05

ISSN： 1344-3542
電解析出アルギン酸ゲルを利用した微小環境における細胞培養

小沢文智, 伊野浩介, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム学会研究会講演要旨集　27th　59　2013/05/23
Electrochemical measurement of alkaline phosphatase activity to evaluate developmental potential of mouse embryonic stem (ES) cells

SHIKU Hitoshi, ARAI Toshiharu, NISHIJO Taku, ZHOU Yuanshu, INO Kosuke, MATSUE Tomokazu

Technical report of IEICE. OME　113　(18)　93-96　2013/04/25
Publisher: The Institute of Electronics, Information and Communication Engineers
ISSN： 0913-5685

More details Close

Electrochemical imaging techniques including scanning electrochemical microscopy (SECM) and local redox cycling-based electrochemical (LRC-EC) chip device have been applied for quantitative evaluation of alkaline phosphatase (ALP) activity of mouse embryonic stem (ES) cells to judge the state of undifferentiation of the cellular samples. Our techniques allowed rapid and high-throughput measuring procedures for ALP activity of stem cells which remarkably improved the invasiveness against the undifferentiated cellular samples. The ES cells and embryoid bodies (EBs) that have been used for ALP assay were found to be re-usable for further cultivation to induce differentiation into cardiomyocyte-like cells. We also mention about background researches done by us and other groups including non-invasive measurement of respiration activity of embryos and EBs for quality control.
電解析出ハイドロゲルマイクロウェルを用いた三次元培養法の開発

小沢文智, 伊野浩介, 新井俊陽, 珠玖仁, 末永智一

電気化学会大会講演要旨集　80th　318　2013/03/29
超高感度化に向けたナノ空間を有する多点電気化学デバイスの開発

菅野佑介, 伊野浩介, 西條拓, 小沢文智, 珠玖仁, 末永智一

電気化学会大会講演要旨集　80th　394　2013/03/29
2P-167 Three-dimensional cell culture using electrodeposition of alginate gels

Ino Kosuke, Ozawa Fumisato, Shiku Hitoshi, Matsue Tomokazu

日本生物工学会大会講演要旨集　65　146-146　2013
Publisher: 日本生物工学会
安価な簡易型センサの実用化に向けた電気化学エンドトキシン検出法の開発 Invited

井上(安田)久美, 髙野真一朗, 高橋里子, 石田洋祐, 伊藤(佐々木, 隆広, 伊野浩介, 珠玖仁, 末永智一

エンドトキシン・自然免疫研究　16　7-11　2013
Bio Sensing and Imaging by Using Mirco/Nano Electrode System

高橋康史, 珠玖仁, 末永智一

表面科学　34　(9)　488-493 (J-STAGE)-493　2013
Publisher: The Surface Science Society of Japan
DOI： 10.1380/jsssj.34.488 　

ISSN： 0388-5321

More details Close

The micro/nanoelectrode has unique characteristics, which are not expected for conventional electrodes, such as the capability to perform localized measurements, low double layer charging currents, low ohmic drops, and fast mass transport. Scanning electrochemical microscopy (SECM) with a micro/nanoelectrode for detecting electroactive chemical species and is an effective tool for the investigation of the localized chemical properties of sample surfaces and interfaces. Because SECM has high temporal resolution under physiological conditions, it is particularly well suited for quantitative measurements of chemicals like neurotransmitters, nitric oxide, reactive oxygen species, and oxygen, which are released/consumed by living cells. This article presents an overview of the recent progress of SECM.
局所レドックスサイクリングを利用した電気化学多点計測デバイスの開発

末永智一, 西條拓, SEN Mustafa, 珠玖仁, 伊野浩介

ポーラログラフィー　58　(3)　174-174　2012/11/21

ISSN： 0034-6691
細胞内での分子反応を計測する―受精卵、細胞塊、組織の１細胞分析技術 Invited Peer-reviewed

珠玖仁, 末永智一

Journal of Japanese Society for Biomaterials　30　(3)　153-158　2012/08
Publisher: Journal of Japanese Society for Biomaterials7
高感度イメージングシステムとレーザーマイクロダイセクションを組み合わせた微小組織血管新生評価システムの有用性に関する検証

西村隆一, 西岡翔, 珠玖仁, 島田美貴, 関口悟, 藤盛啓成, 牛山明, 末永智一, 里見進, 後藤昌史

日本組織移植学会雑誌　11　(1)　42-42　2012/07
Publisher: 日本組織移植学会
ISSN： 1347-6491
「使える解析テクニック！」シリーズ、マイクロ電極（3）応用計測事例：電気化学顕微鏡(SECM)-走査型イオンコンダクタンス顕微鏡(SICM)複合システムの開発

高橋康史, 珠玖仁, 末永智一

Electrochemistry　80　(4)　271-275　2012/04
Publisher: The Electrochemical Society of Japan
DOI： 10.5796/electrochemistry.80.271 　

ISSN： 1344-3542
C-13-3 多点計測デバイスによる電気化学バイオイメージング(C-13.有機エレクトロニクス,一般セッション)

珠玖仁, 伊野浩介, 末永智一

電子情報通信学会総合大会講演論文集　2012　(2)　157-157　2012/03/06
Publisher: 一般社団法人電子情報通信学会
ELECTROCHEMICAL CHIP DEVICE FOR DETECTION OF A SECRETED PROTEIN FROM SINGLE CELLS

Proceedings of the Chemical Sensor Symposium　53　52-54　2012/03
Publisher: 電気化学会化学センサ研究会
4Gp13 Comprehensive electrochemical detection using micro/nanogap electrodes for cell analysis

Ino Kosuke, kanno Yusuke, Nishijo Taku, Shiku Hitoshi, Matsue Tomokazu

64　226-226　2012
Publisher: 日本生物工学会
Comprehensive electrochemical imaging with local redox cycling-based electrochemical chip device for evaluation of three-dimensional culture cells Peer-reviewed

Kosuke Ino, Mustafa Sen, Taku Nishijo, Yusuke Kanno, Hitoshi Shiku, Tomokazu Matsue

2012 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)　16-18　2012

ISSN： 2474-378X

eISSN： 2474-3798
A high-throughput assay for evaluation of embryoid bodies using local redox cycling-based electrochemical chip device Peer-reviewed

K. Ino, T. Nishijo, Y. Kanno, H. Shiku, T. Matsue

CHEMICAL SENSORS 10 -AND- MEMS/NEMS 10　50　(12)　205-210　2012

DOI： 10.1149/05012.0205ecst 　

ISSN： 1938-5862
Negative dielectrophoretic particle positioning in a fluidic flow Peer-reviewed

Tomoyuki Yasukawa, Junko Yamada, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue

Intelligent Automation and Soft Computing　18　(2)　201-211　2012

DOI： 10.1080/10798587.2008.10643237 　

ISSN： 2326-005X 1079-8587

eISSN： 2326-005X
走査型電気化学/イオンコンダクタンス顕微鏡を用いた生体試料の高解像度イメージング

末永智一, 高橋康史, 伊野浩介, 珠玖仁, KORCHEV Y. E., MACPHERSON J. V., UNWIN P. R., KLENERMAN D.

ポーラログラフィー　57　(3)　232-232　2011/11/21

ISSN： 0034-6691
電解析出によるアルギン酸カルシウムゲルを用いた管状血管組織の構築

小沢文智, 伊野浩介, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム研究会講演要旨集　24th　48　2011/11/17
ミジンコの呼吸活性を利用した生態毒性評価のための変換濃縮型計測法の開発

小出昌弘, 安川智之, 伊野浩介, 珠玖仁, 鑪迫典久, 水谷文雄, 末永智一

日本化学会バイオテクノロジー部会シンポジウム講演要旨集　14th　76　2011/09/12
酸素の変換濃縮法を応用したミジンコの呼吸活性計測

小出昌弘, 安川智之, 伊野浩介, 珠玖仁, 鑪迫典久, 水谷文雄, 末永智一

化学とマイクロ・ナノシステム研究会講演要旨集　23rd　54　2011/06/10
細胞ドットアレイの誘電泳動パターニングと機能評価

小沢文智, 伊野浩介, 珠玖仁, 末永智一

電気化学会大会講演要旨集　78th(CD-ROM)　ROMBUNNO.1D09　2011/03/29
マイクロ培養システムによる環境保全に資する難培養性微生物の同定と単離に関する研究

珠玖仁, 末永智一, 板山朋聡

ゼネラル石油研究奨励財団研究報告書　(14)　2011
2Lp05 Microelectrode array device for cell imaging

Ino Kosuke, Nishijo Taku, Shiku Hitoshi, Matsue Tomokazu

63　199-199　2011
Publisher: 日本生物工学会
MRNA ANALYSIS OF MULTICELLULAR SPHEROID WITH A SCANNING PROBE MICROSCOPY SYSTEM Peer-reviewed

Hitoshi Shiku, Genki Saito, Yuanshu Zhou, Yoshiko Horiguchi, Ryosuke Takano, Kosuke Ino, Tomokazu Matsue

PROGRESS ON POST-GENOME TECHNOLOGIES AND MODERN NATURAL PRODUCTS, 2011　343-344　2011
新規電極型プローブを用いたスフェロイド由来mRNA発現解析の検討

斉藤元気, 伊野浩介, 珠玖仁, 末永智一

ポーラログラフィー　56　(3)　144-144　2010/10/19

ISSN： 0034-6691
誘電泳動デバイス上での細胞パターニングの研究

小沢文智, 伊野浩介, 珠玖仁, 末永智一

Rev Polarogr　56　(3)　145　2010/10/19

ISSN： 0034-6691
単一細胞由来mRNAの定量解析

珠玖仁, 伊野浩介, 末永智一

Electrochemistry　78　(10)　832-836　2010/10/01
Publisher: The Electrochemical Society of Japan
DOI： 10.5796/electrochemistry.78.832 　

ISSN： 1344-3542
流路培養による微小環境の再現

珠玖仁

生物工学　88　(10)　533-533　2010/10/01
細胞ドットアレイ作製のための誘電泳動デバイスの開発

小沢文智, 伊野浩介, 珠玖仁, 末永智一

電気化学秋季大会講演要旨集　2010　148　2010/09/02
Electrochemical detection of specific antibodies using microwell array chips

Proceedings of the Chemical Sensor Symposium　50　34-36　2010/09
Publisher: 電気化学会化学センサ研究会
呼吸活性を指標とした胚の品質評価―マウス胚移植試験の成績と産子の正常性について Invited Peer-reviewed

横尾正樹, 伊藤-佐々木隆広, 珠玖仁, 末永智一, 阿部宏之

産婦人科の実際　59　(9)　1375-1379　2010/09
Publisher: 金原出版
ISSN： 0558-4728
誘電泳動による細胞ドットアレイの作製

小沢文智, 伊野浩介, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム研究会講演要旨集　21st　4　2010/06/10
単一細胞由来mRNA回収プローブの開発

珠玖仁, 伊野浩介, 末永智一

シングルセル解析の最前線　223-228　2010/03/28
Publisher: 株式会社シーエムシー出版
Electrochemical detection for endotoxin using recombinant limulus factor C

Proceedings of the Chemical Sensor Symposium　49　136-138　2010/03
Publisher: 電気化学会化学センサ研究会
Scanning Probe Microscopy

SHIKU Hitoshi, MATSUE Tomokazu

61　(2)　187-188　2010/02/01

ISSN： 0915-1869
微生物活性測定のためのマイクロウェルデバイスの作製・評価

小出昌弘, 小出昌弘, 板山朋聡, 安川智之, 伊野浩介, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム研究会講演要旨集　21st　2010
3P-2112 A novel electrochemical device for imaging enzyme activity

INO Kosuke, SAITO Wataru, KOIDE Masahiro, SHIKU Hitoshi, MATSUE Tomokazu

22　176-176　2010
Publisher: 日本生物工学会
MN-P29 Monitoring respiration activity of mouse embryo on an integrated electrochemical microdevice(Section X Micro/Nano Technology for Analysis and Cell Manipulation)

Shiku Hitoshi, Takano Shinichiro, Date Yasumoto, Ino Kosuke, Abe Hiroyuki, Matsue Tomokazu

Journal of bioscience and bioengineering　108　(1)　S164　2009/11
Publisher: The Society for Biotechnology, Japan
ISSN： 1389-1723
Quantitative characterization of gene expression at single cell level combining reporter system with RT real-time PCR

Hitoshi Shiku, Daisuke Okazaki, Kosuke Ino, Tomokazu Matsue

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　108　S149-S150　2009/11

DOI： 10.1016/j.jbiosc.2009.08.401 　

ISSN： 1389-1723
Monitoring respiration activity of mouse embryo on an integrated electrochemical microdevice

Hitoshi Shiku, Shinichiro Takano, Yasumoto Date, Kosuke Ino, Hiroyuki Abe, Tomokazu Matsue

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　108　S164-S164　2009/11

DOI： 10.1016/j.jbiosc.2009.08.442 　

ISSN： 1389-1723
微小電極アレイを組み込んだバイオチップデバイスによる新規多点電気化学検出

伊野浩介, 堀口佳子, 林振字, 珠玖仁, 末永智一

トピックスシーズ集（第61回日本生物工学会大会）　38-39　2009/09/25
誘電泳動による生体微粒子のドットアレイパターン形成

小沢文智, 伊野浩介, 珠玖仁, 末永智一

化学系学協会東北大会プログラムおよび講演予稿集　2009　136　2009/09/19
電気化学的多点計測デバイス Invited Peer-reviewed

珠玖仁, 伊野浩介, 末永智一

未来材料　9　(8)　56-61　2009/08
Publisher: エヌ・ティー・エス
ISSN： 1346-0986
Effects of maternal ageing on mitochondrial activities of mouse oocytes

YOKOO Masaki, KIMURA Naoko, SHIKU Hitoshi, MATSUE Tomokazu, ABE Hiroyuki

26　(2)　S49　2009/04/01

ISSN： 1341-7738
誘電泳動を用いた生体微粒子のアレイパターニング

伊野浩介, 小沢文智, 珠玖仁, 安川智之, 末永智一

電気化学会大会講演要旨集　76th　282　2009/03/29
Cell-based biosensor for detecting toxic activities using leucocytes

Proceedings of the Chemical Sensor Symposium　47　151-153　2009/03
Publisher: 電気化学会化学センサ研究会
HERMETICALLY SEALED ELECTROCHEMICAL MICROWELL-DEVICE FOR MEASUREMENT OF MICROORGANISM ACTIVITY

小出昌弘, 小出昌弘, 小出昌弘, 板山朋聡, 久米博, 伊野浩介, 珠玖仁, 末永智一

Chemical Sensors　25　(Supplement B)　2009
誘電泳動による微粒子集積化技術の免疫測定への応用

安川智之, 安川智之, LEE Hyun Jung, 珠玖仁, 末永智一, 水谷文雄

化学とマイクロ・ナノシステム研究会講演要旨集　19th　2009
2Ip16 A novel lab-on-a-chip device incorporating microelectrode arrays for multi-detection systems

INO Kosuke, HORIGUCHI Yoshiko, LIN Zhenyu, SHIKU Hitoshi, MATSUE Tomokazu

21　129-129　2009
Publisher: 日本生物工学会
DEVELOPMENT OF MULTI-FUNCTIONAL PROBES FOR GENE EXPRESSION ANALYSIS AT SIGLE CELL LEVEL Peer-reviewed

Hitoshi Shiku, Takeshi Yamakawa, Daisuke Okazaki, Genki Saito, Yasumoto Date, Kosuke Ino, Tomokazu Matsue

IFPT'6: PROGRESS ON POST-GENOME TECHNOLOGIES, PROCEEDINGS　261-263　2009
バイオチップの走査型電気化学顕微鏡解析

安川智之, 珠玖仁, 水谷文雄, 末永智一

表面技術　59　(12)　818-824　2008/12
Publisher: The Surface Finishing Society of Japan
DOI： 10.4139/sfj.59.818 　

ISSN： 0915-1869
One-step sandwich immunosensing by negative dielectrophoresis

Proceedings of the Chemical Sensor Symposium　46　76-78　2008/09
Publisher: 電気化学会化学センサ研究会
微小電極を用いた誘電詠動による三次元細胞パターニング法の開発

伊野浩介, 小沢文智, 安川智之, 珠玖仁, 末永智一

日本生物工学会大会講演要旨集　60th　89　2008/07/11
細胞機能を利用するマイクロバイオセンサ

井上(安田)久美, 珠玖仁, 末永智一

マテリアルインテグレーーション　21　232-241　2008/06
Quality evaluation of embryos cultured in vitro by scanning electrochemical microscopy

伊達安基, 阿部宏之, 珠玖仁, 末永智一

バイオサイエンスとインダストリー　66　(6)　298-299　2008/06
Publisher: 財バイオインダストリー協会
走査型イオンコンダクタンス顕微鏡を用いた細胞膜表面観察と局所電気浸透流インジェクションへの応用

高橋康史, 村上有美, 安川智之, 珠玖仁, 神崎展, 末永智一

電気化学会大会講演要旨集　75th　435　2008/03/29
複合酵素による走査型電気化学顕微鏡の電流シグナルの増幅と細胞膜タンパク質検出への応用

高橋康史, 宮本健史, 安川智之, 珠玖仁, 浅野竜太郎, 熊谷泉, 末永智一

分析化学討論会講演要旨集　69th　2008
Negative dielectrophoretic manipulation with microparticles for rapid immunosensing Peer-reviewed

Tomoyuki Yasukawa, Ramón-Azcón Javier, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue

2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008　372-378　2008

DOI： 10.1109/MHS.2008.4752480 　
Ebpl expression is induced by influenza virus infection Peer-reviewed

Miho Ejima, Ryuta Ueda, Shinichiro Kume, Daisuke Okazaki, Takefumi Yamakawa, Hitoshi Shiku, Ayae Honda

2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008　214-218　2008

DOI： 10.1109/MHS.2008.4752452 　
Measurement of the Respiratory Activity of Single Human Embryos by Scanning Electrochemical Microscopy Peer-reviewed

Hiroyuki Abe, Masaki Yokoo, Takahiro Itoh-Sasaki, Megumi Nasu, Kaori Goto, Yoko Kumasako, Yasuhisa Araki, Hitoshi Shiku, Tomokazu Matsue, Takafumi Utsunomiya

TRANSACTIONS OF THE MATERIALS RESEARCH SOCIETY OF JAPAN, VOL 33, NO 3　33　(3)　759-762　2008
Multiple Analysis of Respiratory Activity in the Identical Oocytes by Applying Scanning Electrochemical Microscopy Peer-reviewed

Masaki Yokoo, Takahiro Ito-Sasaki, Hitoshi Shiku, Tomokazu Matsue, Hiroyuki Abe

TRANSACTIONS OF THE MATERIALS RESEARCH SOCIETY OF JAPAN, VOL 33, NO 3　33　(3)　763-766　2008
A lab-on-a-chip device for electrochemical detection of endocrine disruptors using genetically-engineered yeast cells

Matsue Tomokazu, Kosuke Ino, Hitoshi Shiku

Chemical sensors　24　(4)　168-173　2008
Publisher: 化学センサ研究会
Development of a microwell culture device for individual embryo

DATE Yasumoto, YOKOO Masaki, ITOH SASAKI Takahiro, SHIKU Hitoshi, ABE Hiroyuki, MATSUE Tomokazu

24　(2)　S65　2007/04/01

ISSN： 1341-7738
Assessment of embryo culture medium for ART using by electrochemical respiration measuring system

YOKOO Masaki, ITO SASAKI Takahiro, DATE Yasumoto, SHIKU Hitoshi, MATSUE Tomokazu, ABE Hiroyuki

24　(2)　S49　2007/04/01

ISSN： 1341-7738
Analysis of mitochondrial function in mouse embryos during early embryonic development

OKAZAKI Naoto, YOKOO Masaki, ITO SASAKI Takahiro, YASUKAWA Tomoyuki, SHIKU Hitoshi, ABE Hiroyuki, MATSUE Tomokazu

24　(2)　S47　2007/04/01

ISSN： 1341-7738
細胞分析電気化学デバイスとマイクロシステムの開発

珠玖仁, 梨本裕司, 鳥澤勇介, 安川智之, 末永智一

日本化学会バイオテクノロジー部会 NEWS LETTER　10　(2)　7-8　2007/02/28
Publisher: 社団法日本化学会バイオテクノロジー部会
単一細胞由来mRNA回収マイクロプローブの開発

珠玖仁, 梨本裕司, 高橋康史, 安川智之, 阿部宏之, 末永智一

日本化学会講演予稿集　87th　(2)　2007

ISSN： 0285-7626
誘電泳動を利用した膜タンパク質の同定と細胞分離

安川智之, 鈴木雅登, 山田純子, 珠玖仁, 末永智一

表面技術協会講演大会講演要旨集　115th　2007

ISSN： 0917-2947
多機能ナノ電気化学顕微鏡を用いた単一生細胞表面の膜タンパク質局所イメージング

高橋康史, 宮本健史, 安川智之, 珠玖仁, 浅野竜太郎, 熊谷泉, 末永智一

電気化学秋季大会講演要旨集　2007　2007
高感度ナノ電気化学顕微鏡を用いた細胞膜タンパク質のイメージング

高橋康史, 宮本健史, 安川智之, 珠玖仁, 浅野竜太郎, 熊谷泉, 末永智一

応用物理学会学術講演会講演予稿集　68th　(3)　2007
Oxygen consumption of mammalian embryos and Oocytes monitored by scanning electrochemical microscopy Peer-reviewed

Hitoshi Shiku, Tomoyuki Yasukawa, Tornokazu Matsue, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe

2007 IEEE SENSORS, VOLS 1-3　1408-1411　2007

ISSN： 1930-0395
Respiratory activity and ultrastructural features of bovine somatic nuclear transfer embryos

H. Abe, K. Aoyagi, S. Aoyagi, H. Shiku, T. Matsue, Y. Sendai, H. Hoshi

REPRODUCTION FERTILITY AND DEVELOPMENT　19　(1)　129-130　2007

ISSN： 1031-3613
Dual - Capillary micro - Fluidic probe to collect mRNA from a single adherent cells Peer-reviewed

H. Shiku, T. Yamakawa, Y. Nashimoto, Y. Takahashi, Y. Torisawa, T. Yasukawa, T. Ito-Sasaki, M. Yokoo, H. Abe, H. Kambara, T. Matsue

PROGRESS ON POST-GENOME TECHNOLOGIES　61-64　2007
Micropatterning with different cell types by dielectrophoretic manipulation Peer-reviewed

Tomoyuki Yasukawa, Masato Suzuki, Hitoshi Shiku, Tomokazu Matsue

2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE, VOLS 1 AND 2　179-182　2007

DOI： 10.1109/MHS.2007.4420848 　

ISSN： 2474-378X

eISSN： 2474-3798
The efficient maintenance of oxygen consumption by porcine oocytes relates to the high competence of oocyte maturation. Peer-reviewed

Yokoo M, Ito-Sasaki T, Shiku H, Matsue T, Aoyagi S, Hoshi H, Abe H

REPRODUCTION FERTILITY AND DEVELOPMENT　19　295-295　2007
Estimation of respiratory activity of mouse embryos by scanning electrochemical microscopy Peer-reviewed

Ito-Sasaki Takahiro, Okazaki Naoto, Yokoo Masaki, Shiku Hitoshi, Yasukawa Tomoyuki, Matsue Tomokazu, Abe Hiroyuki

ZOOLOGICAL SCIENCE　23　(12)　1185　2006/12

ISSN： 0289-0003
Protein Patterning Techniques and Analysis of Interfaces Phenomenon

高橋康史, 珠玖仁, 安川智之, 末永智一

表面科学　27　(10)　613-616　2006/10

DOI： 10.1380/jsssj.27.613 　
「電気化学：測定と解析のてびき」プローブ法（１）走査型電気化学顕微鏡(SECM)・走査型振動電極法(SVET)

青柳重夫, 内海陽介, 福泉敦尚, 珠玖仁, 末永智一

Electrochemictry　74　(9)　787-790　2006/09
Publisher: The Electrochemical Society of Japan
DOI： 10.5796/electrochemistry.74.787 　

ISSN： 1344-3542
Relationship between respiration activity and competence for in vitro maturation in porcine oocyte

YOKOO Masaki, SASAKI Takahiro, SHIKU Hitoshi, MATSUE Tomokazu, AOYAGI Shigeo, HOSHI Hiroyoshi, ABE Hiroyuki

23　(2)　S59　2006/04/01

ISSN： 1341-7738
Quality evaluation of bovine oocytes : Relationship between oocyte quality and respiration activity

SAITO Takeshi, ABE Hiroyuki, YOKOO Masaki, SASAKI Takahiro, HOSHI Hiroyoshi, YASUKAWA Tomoyuki, SHIKU Hitoshi, MATSUE Tomokazu

23　(2)　S60　2006/04/01

ISSN： 1341-7738
Electrochemical microbiosensors using living cells

T Matsue, H Shiku

ELECTROCHEMISTRY　74　(2)　107-113　2006/02

DOI： 10.5796/electrochemistry.74.107 　

ISSN： 1344-3542
Fabrication and electrochemical characterization of a microcfluidic device with a separated reference electrode

長峯邦明, 沼井大輔, 安川智之, 葛西重信, 珠玖仁, 小出昌弘, 板山朋聡, 末永智一

日本化学会講演予稿集　86th　(1)　2006

ISSN： 0285-7626
シアフォース電気化学・近接場光学顕微鏡の開発とバイオイメージングへの応用

高橋康史, 平野悠, 安川智之, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム研究会講演要旨集　13th　2006
酵素修飾ナノ微粒子を固定化したマイクロセンサの作製と評価

安川智之, 沼井大輔, 長峯邦明, 珠玖仁, 末永智一

表面技術協会講演大会講演要旨集　113th　2006

ISSN： 0917-2947
泳動電着法を利用したマイクロ電極表面の選択的酵素修飾

須藤淳, 安川智之, 鈴木雅登, 珠玖仁, 末永智一

電気化学秋季大会講演要旨集　2006　2006
細胞・微生物チップを用いた電気化学的レポーターアッセイ

長峯邦明, 栗原愛, 小野寺志穂, 鳥沢勇介, 安川智之, 珠玖仁, 末永智一, 浅野竜太郎, 熊谷泉

電気化学会大会講演要旨集　73rd　2006
交互くし型電極を用いたanti-mouse IgG修飾微粒子の誘電泳動特性の評価

須藤淳, 安川智之, 鈴木雅登, 珠玖仁, 末永智一

電気化学会大会講演要旨集　73rd　2006
シアフォース電気化学/近接場光顕微鏡によるリアルタイム生体イメージング

高橋康史, 平野悠, 安川智之, 珠玖仁, 末永智一

電気化学会大会講演要旨集　73rd　2006
1B16-1 Construction of single bacterial cell arrays for electrochemical gene function analysis

NAGAMINE Kuniaki, GOTO Shun, YASUKAWA Tomoyuki, SHIKU Hitoshi, MATSUE Tomokazu

18　25-25　2006
Publisher: 日本生物工学会
Analysis of respiratory activity of single bovine oocytes by scanning electrochemical microscopy Peer-reviewed

Hiroyuki Abe, Takeshi Saito, Hitoshi Shiku, Masaki Yokoo, Itoh-Sasaki Takahiro, Hiroyoshi Hoshi, Tomokazu Matsue

Progress on Post-Genome Technologies　19-22　2006
Microsystem surveying three dimensional (3D) culture and embryo development Peer-reviewed

H. Shiku, Y. Nashimoto, Y. Torisawa, T. Yasukawa, T. Saito, T. Ito-Sasaki, M. Yokoo, H. Abe, T. Matsue

Progress on Post-Genome Technologies　221-223　2006
Development of negative dielectrophoretic cellular patterning system for living cells Peer-reviewed

Masato Suzuki, Tomoyuki Yasukawa, Mariko Komabayashi, Akiko Inagaki, Yoshio Hor, Hitoshi Shiku, Tomokazu Matsue

2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS　138-+　2006

DOI： 10.1109/MHS.2006.320230 　

ISSN： 2474-378X
電気化学・測定法と解析の手引きプローブ法（１）

青柳重夫, 内海陽介, 福泉敦尚, 珠玖仁, 末永智一

電気化学　2006
Oxygen consumption during oocyte maturation in bovine cumulus-oocyte complexes and oocytes cultured either in serum-free medium or serum-containing medium

ABE Hiroyuki, KACCHI Masayuki, YAMASHITA Shoko, SHIKU Hitoshi, MATSUE Tomokazu, AOYAGI Shigeo, HOSHI Hiroyoshi

22　(2)　S34　2005/04/01

ISSN： 1341-7738
HERMETICALLY SEALED ELECTROCHEMICAL MICROWELL ARRAY CHIP FOR SINGLE CELL ANALYSIS

沼井大輔, 長峯邦明, 安川智之, 珠玖仁, 小出昌弘, 板山朋聡, 末永智一

Chemical Sensors　21　(Supplement B)　2005
有毒藍藻類のマイクロ電気化学測定による活性測定技術の開発

小出昌弘, 稲森悠平, 長峯邦明, 安川智之, 沼井大輔, 珠玖仁, 末永智一, 板山朋聡

日本水処理生物学会誌別巻　(25)　2005

ISSN： 0910-6766
微小電極を組み込んだ密閉型ウエル中での藻類の光合成活性計測

長峯邦明, 安川智之, 沼井大輔, 珠玖仁, 末永智一, 小出昌弘, 板山朋聡

化学とマイクロ・ナノシステム研究会講演要旨集　11th　2005
大腸菌を宿主とした遺伝子および組み換え蛋白質発現スクリーニングチップの開発

小野寺志穂, 長峯邦明, 安川智之, 珠玖仁, 末永智一

エレクトロオーガニックケミストリー討論会講演要旨集　29th　2005
流路・電極集積化型細胞チップの作製とリアルタイム活性評価への応用

鳥沢勇介, 松井伸人, 梨本裕司, 安川智之, 珠玖仁, 末永智一

化学とマイクロ・ナノシステム研究会講演要旨集　11th　2005
OXYGEN PERMEATBILITY FOR POLY(DIMETHYLSILOXANE) (PDMS) CHARACTERIZED BY FEEDBACK-MODE SCANNING ELECTROCHAMICAL MICROSCOPY

珠玖仁, 斉藤剛史, WU Ching-Chou, 安川智之, 末永智一, 末永智一, 星宏良, 横尾正樹, 阿部宏之, 山田弘

Chemical Sensors　21　(Supplement B)　2005
マイクロデバイスを用いたウシ精子の呼吸活性評価

斎藤剛史, 珠玖仁, 呉靖宙, 安川智之, 佐々木隆広, 横尾正樹, 阿部宏之, 星宏良, 星宏良, 末永智一

化学とマイクロ・ナノシステム研究会講演要旨集　12th(CD-ROM)　2005
2D09-1 Transformation of E.coil cell microarrays with microfabricated device

NAGAMINE Kuniaki, ONODERA Shiho, TORISAWA Yu-suke, YASUKAWA Tomoyuki, SHIKU Hhtoshi, MATSUE Tomokazu

17　128-128　2005
Publisher: 日本生物工学会
電気化学オンチップ遺伝子工学 : オンチップ培養とその評価 Invited Peer-reviewed

彼谷高敏, 珠玖仁, 安川智之, 末永智一

ぶんせき　(359)　670-672　2004/11
Publisher: 日本分析化学会
ISSN： 0386-2178
電気化学オンチップ遺伝子工学：オンチップ培養とその評価

彼谷高敏, 珠玖仁, 安川智之, 末永智一

ぶんせき　2004　(11)　671-673　2004/11
Bioassay using living cells integrated on a chip

YS Torisawa, H Shiku, T Yasukawa, T Matsue

BUNSEKI KAGAKU　53　(5)　367-382　2004/05

ISSN： 0525-1931
細胞チップを用いるバイオアッセイ

鳥澤勇介, 珠玖仁, 安川智之, 末永智一

Bunsei Kagaku　53　(5)　367-382　2004/05
Publisher: The Japan Society for Analytical Chemistry
DOI： 10.2116/bunsekikagaku.53.367 　

ISSN： 0525-1931

More details Close

A living cell-based assay is widely used for the characterization of various cellular functions, screening of drugs and environmental monitoring. Recent progress in cell culture and microfabrication technologies has attracted the integration of cell culture and sensors on a chip. A bioassay using a cell chip makes it possible to evaluate small-volume samples with multitudinous characterizations, and is applicable for a rapid and simple detection system. This paper reviews cell-based bioassay using living cells integrated on a chip. The cell patterning and three-dimensional cell culture facilitates the integration of cell culture technology. These in vitro culture technologies are appropriate for the recapitulation of in vivo-like cell behavior, because of the potential to control the cell differentiation and polarity. Alternatively, the evaluation methods, such as cellular acidification, oxygen consumption and impedance measurements, are principally non-invasive, and are especially expected in the field of anticancer drug sensitivity test using a biopsy tumor.
Oxygen consumption of bovine oocytes and cumulus cells during in vitro maturation

ABE Hiroyuki, KACCHI Masayuki, SHIKU Hitoshi, MATSUE Tomokazu, AOYAGI Shigeo, HOSHI Hiroyoshi

21　(2)　S17　2004/04/01

ISSN： 1341-7738
東北支部平成15年度活動報告

珠玖仁, 末永智一

表面技術 = The Journal of the Surface Finishing Society of Japan　55　(2)　137-138　2004/02/01

ISSN： 0915-1869
走査型電気化学顕微鏡のセンサへの利用と距離制御による高解像度化

平野悠, 小谷松大祐, 安川智之, 珠玖仁, 末永智一

Electrochemictry　72　(2)　137-142　2004/02
単一微生物細胞のパターニング及び活性評価法の開発

沼井大輔, 長峯邦明, 板山朋聡, 荒川智之, 珠玖仁, 末永智一

化学系学協会東北大会プログラムおよび講演予稿集　2004　2004
増殖・発現アッセイを可能にする細胞・微生物チップ

珠玖仁, 長峯邦明, 鳥沢勇介, 松井伸人, 彼谷高敏, 安川智之, 末永智一

日本化学会バイオテクノロジー部会シンポジウム講演要旨集　8th　2004
DEVELOPMENT OF SCANNING ELECTROCHEMICAL MICROSCOPY TO MEASURE THE RESPIRATION OF MAMMALIAN EMBRYOS(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) :

Abe Hiroyuki, Shiku Hitoshi, Aoyagi Shigeo, Utsumi Yosuke, Hoshi Hiroyoshi, Matsue Tomokazu

Zoological science　21　(12)　1295-1295　2004
Publisher: Zoological Society of Japan
ISSN： 0289-0003
In vitro culture and evaluation techniques of embryos for production of high quality bovine embryos

H. Abe, H. Shiku, S. Aoyagi, H. Hoshi

J. Mamm. Ova Res　21　(1)　22-30　2004
Publisher: J. Mamm. Ova Res
DOI： 10.1274/jmor.21.22 　

ISSN： 1341-7738
電気化学顕微鏡を用いた細胞計測法の実用化をめざして

安川智之, 鳥澤勇介, 珠玖仁, 末永智一

Seibutsu Butsuri　44　(1)　36-39　2004/01
Publisher: 生物物理
DOI： 10.2142/biophys.44.36 　

ISSN： 0582-4052
電気泳動および誘電泳動を利用したマイクロチャンネル内における微粒子の流動制御

尾形幸子, 安川智之, 珠玖仁

Proceedings of the Chemical Sensor Symposium　37　10-12　2003/09
Publisher: 電気化学会化学センサ研究会
Characterization of single embryos with SECM.

T Matsue, H Shiku

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　226　U131-U131　2003/09

ISSN： 0065-7727
Quantification of oxygen consumption of bovne embryos developed from in Vitro-matured and fertilized oocytes by a scanning electrochemical microscopy

ABE Hiroyuki, SHIKU Hitoshi, AOYAGI Shigeo, UTSUMI Yousuke, MATSUDAIRA Masaaki, SHIRAISHI Takuo, HOSHI Hiroyoshi

20　(2)　S65　2003/08/01

ISSN： 1341-7738
抗体アレイチップを用いた高感度電気化学イムノアッセイデバイスの開発

小笠原大知, 平野悠, 安川智之, 珠玖仁, 末永智一, 小堀樹一郎, 牛沢幸司, 川端荘平

化学系学協会連合東北地方大会プログラムおよび講演予稿集　2003　2003
ウシ胚におけるミトコンドリアの形態変化と胚の品質評価

阿部宏之, 珠玖仁, 星宏良

日本胚移植学雑誌　24　(1)　19-27　2002/01
走査型電気化学顕微鏡(SECM)/共焦点顕微鏡(CFM)システムを用いた固定化酵素の酵素活性と蛍光の同時イメージング

金谷典大, 珠玖仁, 小谷松大祐, 西澤松彦, 末永智一

バイオイメージング　10　(3)　186-187　2001/10/01

ISSN： 0919-4916
測定法講座/その場測定法/走査型電気化学顕微鏡

珠玖仁, 大矢博昭, 末永智一

Electrochemistry　69　(10)　806-810　2001/10
電気化学顕微鏡の基礎と応用

珠玖仁, 大矢博昭, 末永智一

表面技術　51　(1)　46-52　2000/01

DOI： 10.4139/sfj.51.46 　
微小空間の電気化学—走査型電気化学顕微鏡による生体分子機能表面の構築と評価

珠玖仁, 大矢博昭, 末永智一

化学　54　(11)　72-73　1999/11
Probing biological systems with near-field optics

H Shiku, CW Hollars, MA Lee, CE Talley, G Cooksey, RC Dunn

LASER TECHNIQUES FOR CONDENSED-PHASE AND BIOLOGICAL SYSTEMS　3273　156-164　1998

DOI： 10.1117/12.306124 　

ISSN： 0277-786X
Microfabrication and characterization of solid surfaces patterned with enzymes or antigen-antibodies by scanning electrochemical microscopy

H Shiku, Y Hara, T Takeda, T Matsue, Uchida, I

SOLID-LIQUID ELECTROCHEMICAL INTERFACES　656　202-209　1997

ISSN： 0097-6156
Ion and electron transport across a planar bilayer lipid membrane

T. Matsue, H. Yamada, H. Shiku, I. Uchida

Redox Mechanism and Interfacial Properties of Molecules of Biological Importance　93-11　290-298　1993/10

Show all ︎Show first 5

Books and Other Publications 13

Encyclopedia of Electrochemistry(Chapter 8 (Scanning electrochemical microscopy applied to biological systems))

H. Shiku, H. Ohya, T. Matsue

Encyclopedia of Electrochemistry, Wiley-VCH　2002/04
基礎からわかる物理化学

柴田茂雄, 珠玖仁, 須藤祐子, 長尾大輔, 松本祐司, 米本年邦

共立出版　2022/04

ISBN: 9784320044999
三次元培養における培養手法と周辺技術動向

梨本裕司, 伊野浩介, 平典子, 珠玖仁

情報機構（東京）　2019/04

ISBN: 9784865021684
Nanobiosensors and Nanobioanalyses

Y. Takahashi, H. Shiku, T. Matsue

Springer Japan　2015/12

ISBN: 9784431551898
電気化学ナノイメージング、in監修民谷栄一，“ナノ融合による先進バイオデバイス”第4章3節PP.156-162

珠玖仁, 末永智一, シーエムシー出版, 日発行

2011/11/30
酵素利用技術体系-基礎・解析から改変・高機能化・産業利用まで-, 走査型電気化学顕微鏡による酵素イメージング, p. 112-116（第２編、第２節）

末永智一, 高橋康史, 伊野浩介, 珠玖仁

株式会社エヌ・ティー・エス　2010/04/16

More details Close

監修小宮山眞
“シングルセル解析の最前線”, 単一細胞由来mRNA回収プローブの開発、（第4章3節PP.223-228 (9ページ)）、

珠玖仁, 伊野浩介, 末永智一

株式会社シーエムシー出版　2010/03
細胞機能を利用するマイクロバイオセンサ in 先進化学センサ（第２章、第１節）

井上(安田)久美, 珠玖仁, 末永智一

株式会社ティー・アイ・シィー出版　2008/06
Bioelectrochemistry: fundamentals, experimental techniques, and applications, (Eds. P.N. Bartlett) (Chapter 7,Whole-cell biosensors,p. 249-266)

H. Shiku, K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue

John Wiley & Sons Ltd., West Sussex, UK.　2008/04/11

ISBN: 9780470843642
バイオ電気化学の実際-バイオセンサ・バイオ電池の実用展開-（第13章（生体分子の局所分析と電気化学イメージング）p.207-227）

高橋康史, 珠玖仁, 安川智之, 末永智一

シーエムシー出版　2007/03/31

More details Close

監修池田篤治
一細胞定量解析の最前線‐ライフサーベイヤ構築に向けて‐（第5章4節細胞操作のデバイスとマイクロシステム）

珠玖仁, 末永智一

シーエムシー出版　2006/12/27
バイオチップの最新技術と応用（4章第1節（細胞・微生物チップの開発）p.179-191）

彼谷高敏, 安川智之, 珠玖仁, 末永智一

シーエムシー出版　2004/06
Microamperometric sensing of localized biomolecules

H. Shiku, T. Matsue

Sensors Update Vol. 6, Wiley-VCH　2000/01

Show all Show first 5

Industrial Property Rights 4

電気化学的多検体分析方法

青柳重夫, 松平昌昭, 内海陽介, 珠玖仁, 白石卓夫, 末永智一, 阿部宏之, 星宏良

第3688671号

Property Type: Patent
哺乳動物胚の無侵襲的品質評価方法及びその装置

珠玖仁, 白石卓夫, 大矢博昭, 末永智一, 阿部宏之, 星宏良

第3693907号

Property Type: Patent
凸状領域を用いた分析方法

末永智一, 珠玖仁, 内田勇

第3764779号

Property Type: Patent
検体セルおよび電気化学的分析装置及び電気化学的分析方法

珠玖仁, 白石卓夫, 末永智一, 阿部宏之, 星宏良, 青柳重夫, 松平昌昭, 内海陽介

特許第4097492号

Property Type: Patent

Research Projects 20

Reconstruction of tumor microenvironment and in-situ metabolic analysis

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

2021/04/01 - 2024/03/31
Electrochemical imaging that contributes to cell lineage determination

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2021/04/01 - 2024/03/31
Monitoring system for dynamic change of bio-medical materials with scanning electrochemical microscopy

Shiku Hitoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2018/04/01 - 2021/03/31

More details Close

The improved spatial resolution of the scanning electrochemical microscope (SECM) has created a necessity to revisit previously overlooked information. It is extremely important to examine and clarify in detail the interaction between the probe and the substrate, which has become apparent due to the improvement in spatial resolution, and the surface charge on the probe and the sample surface. In this research project, the correlation between the topography and the electrochemical image obtained by SECM system. We will develop a scanning probe microscopy (SPM) system that enables comprehensive gene expression analysis by acquiring high-resolution images of tissue models and collecting trace amounts of samples from the targeted cells. The changes in the imaging data over time will be recorded by SPM and optical microscope observation.
Large-scale multi-analyte sensing system with bipolar electrode array

Shiku Hitoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2016/04/01 - 2018/03/31

More details Close

We have developed a multi-analyte sensing platform based on bipolar electrode (BPE) array device to realize small size, power saving, highly sensitive, and rapid screening system. BPE array allows wireless controlling individual redox reactions at conductive plate. Autonomous and novel large-scale multi-analyte sensing system is designed, by addressing various combination of different types of biomolecules, including nucleic acids and proteins, and redox couples with different electrode potentials. Electrochemical bioimaging instrument we have developed has been applied for mapping of localized surface charge or current.
electrochemical control of mas transfer and single cell analysis by multifunctional nanopipet

Shiku Hitoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2015/04/01 - 2018/03/31

More details Close

Recently, single-cell analysis has been developed to achieve highly sensitive and high-throughput system. Large-scale data analysis of gene expression profiles for actual tissue samples of colorectal cancer patients. However, in the present stage, expensive cell sorter is required to perform single-cell analysis of tissue samples. And the cell sorting process lose information about the original location of the single cells in the tissue samples. We have developed a single-cell analytical tool based on scanning probe microscopy to obtain high-resolution topographic images of tissue model. Sequentially, the SPM system allows to precisely collect a targeted single-cell or a part of cytosol sample from a single-cell for high-throughput gene expression analysis.
Innovative electrochemical imaging device for bioanalysis

Matsue Tomokazu, INO KOSUKE, TAKAHASHI YASUFUMI, RAMON JAVIER, SHIKU HITOSHI, INOUE KUMI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2013/05/31 - 2016/03/31

More details Close

In the present study, we have developed two-types of electrochemical devices for bioimaging. One was a bio-LSI device which was based on large-scale integrated (LSI) circuits dedicated to bioanalysis. The other was a local redox cycling-based electrochemical (LRC-EC) device utilizing local redox cycling. The device consisted of many switching sensors incorporated into a small chip device to detect electrochemical responses. These bioimaging devices were successfully applied for evaluation of oxygen consumption and secreted chemicals from cells, and cell differentiation.
Novel electrode array device for electrochemical imaging

MATSUE Tomokazu, SHIKU Hitoshi, INO Kosuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2010 - 2012

More details Close

In this study, we have proposed a novel method for electrochemical imaging using local redox cycling-based electrochemical (LRC-EC) devices and systems. In the device, two arrays of microelectrodes are orthogonally arranged to fabricate an n × n array of crossing points (measurement points) with only 2n bonding pads for external connection. The crossing points of the column and row electrodes can easily be addressed by setting the proper potentials at the column and row electrodes to induce local redox cycling at desired crossing points. Therefore, the crossing points can be used as individual electrochemical sensors. This addressable and multiple detection system successfully applied for the detection of enzyme activity, gene-expression analysis, cell differentiation analysis, DNA detection and dynamic analyses of droplets. The present LRC-EC system will be widely applicable for various bioimaging and biosensing.
Analysis of mitochondrial function in mammalian oocytes using electrochemical microscopy

ABE Hiroyuki, YOKOO Masaki, SHIKU Hitoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Yamagata University

2009 - 2012

More details Close

Oxygen consumption is a ubiquitous parameter which can provide valuable informationon metabolic mechanisms, and oocyte and embryo quality. The aim of this study was 1) todevelop a device to measure cellular respiration of single oocytes and embryos based onscanning electrochemical microscopy (SECM) and 2) to assess the mitochondrial respiration function, such as oxygen consumption and gene expression of cytochrome coxidase (Cox) in single oocytes and embryos at different developmental stages. We succeeded in development of a modified SECM measuring system including Pt-microdiskelectrodes and respiration assay medium can monitor the oxygen consumption rate ofsingle oocytes and embryos. Using this system, we can measure respiration activity bysingle embryos and oocytes of bovine and mice at different developmental stages. Development of mitochondrial respiration activity, such as membrane potential of mitochondria and gene expression of Cox, corresponds to the increase of oxygen consumption rates during the development of embryos.
ソフト界面の輸送現象を追跡可能なマニピュレーションシステムの開発

珠玖仁

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 新学術領域研究(研究領域提案型)

Institution: 東北大学

2011 - 2011
1細胞マイクロ分析システム:遺伝子発現から代謝までの階層的理解

珠玖仁

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 若手研究(A)

Institution: 東北大学

2009 - 2011

More details Close

本研究では、1細胞レベルでの核酸(DNA.RNA)、タンパク質の同時定量法を確立し、遺伝子発現から代謝経路を含む階層的理解を目標とする。分析システムの感度向上により、核酸-タンパク質修飾などエピジェネティックな発現制御に係る情報を得る。これにより、マウス初期胚の発生過程、iPS細胞など遺伝子導入系細胞の発現効率の評価、組織培養やマイクロデバイスを用いるオンチップ培養過程で遺伝子発現解析が可能となる。(1)損傷回復モデルおよび同調培養系で1細胞遺伝子発現解析を実施した。一般的に、大量細胞系と1細胞系で、遺伝子発現の結果は極めて良く一致した。損傷復培養では各種成長因子を添加し、スクラッチングにより形成した傷モデルのエッジ部分特異的に、膜受容体およびシグナル伝達の下流に位置するキナーゼのmRNA発現量が大量細胞系-1細胞系間で有意に異なることを見出した。同調培養系では、細胞周期感受性の蛍光プローブレポータータンパク質の発現を蛍光顕微鏡下で観察しながら、mRNA発現を1細胞レベルで定量することを試みた。(2)3次元培養系に適用可能な多機能プローブとして、リング電極-針状電極2電極系で顕微鏡観察下、細胞塊を構成する任意の1細胞を狙って電場破砕→mRNA回収・定量が可能であることを実証した。(3)DNAのGpCメチル化領域を検出するために、簡易型発光検出装置を搭載したパイロシーケンサを用いてDNA配列を解読するための基礎検討を実施した。
ダイナミックな細胞内外輸送現象の1細胞定量解析法

珠玖仁

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 新学術領域研究(研究領域提案型)

Institution: 東北大学

2009 - 2010

More details Close

本研究では、ソフト界面を介する細胞内外輸送現象を1細胞レベルで定量的に解析する手法を開発し、それらを組合せ、遺伝子導入やドラッグデリバリーのメカニズム解明と効高率化に貢献することを目標とする。本年度は、上皮成長因子受容体(EGFR)-EGF結合を介するエンドサイトーシス過程を追跡する。 (1)電気化学イメージングによるエンドサイトーシスのリアルタイムモニタリング:昨年度に引き続き、生細胞の膜表面に存在する上皮成長因子受容体(EGFR)の電気化学イメージングを検討した。生細胞電気化学イメージングから、メナジオンや酸素の細胞内反応・細胞膜透過現象を評価した。金ナノ粒子をプローブとしてEGFRを免疫標識し、光散乱に基づき細胞の内外を識別可能かどうか基礎的検討を実施した。我々が独自に開発した電気化学多点計測デバイスのスループットを大輻に改善・改良できた。 (2)パッチクランプ法:イオン電流をプローブ-サンプル間距離制御に用いるSICM(scanning ion-conductance microscopy)により、単一細胞の形状-電気化学同時イメージングに成功した。電気化学イメージングを可能とする複数タイプのナノプローブを試作し、白金電極に加えて炭素電極タイププロープの開発に成功した。マクロファージに着目し、マクロファージ活性化因子の添加に伴う、形態変化,膜容量変化・活性酸素産生能の同時評価を実施した。 (3)PCRに基づく核酸分析法(1cell RT-PCR):ブローブを改良し1細胞ごとのmRNA回収定量を実施した。アルカリホスファターゼをモデルタンパク質として、回収したタンパク質の酵素活性を電気化学的に評価する流体デバイスの開発を実施した。
Dielectrophoresis-based cell patterning for cell culture chip

INO Kosuke, MATSUE Tomokazu, SHIKU Hitoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (Start-up)

Institution: Tohoku University

2008 - 2009

More details Close

In this study, dielectrophoresis-based cell patterning was developed for cell culture chip. Electrodes were designed and electric field simulations were performed to manipulate bioparticles toward target areas. Based on the simulation results, several kinds of devices were fabricated for the formation of localized and non-uniform electric fields. When an appropriate AC voltage was applied to the electrode, array patterns of microparticles such as polystyrene beads, yeasts and cells were rapidly fabricated on the target substrate with ease. The results suggest that the devices can be widely applied for tissue engineering and drug screening.
Multifunctional Nano-Scanning Electrochemical Microscopy

MATSUE Tomokazu, MIZUTANI Fumio, YANAGISAWA Teruyuki, SHIKU Hitoshi, YASUKAWA Tomoyuki, YAMADA Hiroshi, AKIBA Uichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (S)

Institution: Tohoku University

2006 - 2009

More details Close

The multifunctional micro/nano scanning electrochemical imaging system (NanoSECM) was developed and utilized for the single-cell diagnostics. We fabricated multifunctional micro/nano-structured probe electrodes and developed a novel feedback control between the electrode and sample surfaces at nanometer levels. By using NanoSECM with these new technologies, we successfully attained the fast and high resolution and simultaneous imaging of live cells to disclose the cell functions.
Micro-culture device for environmental condition control and quantitative electrochemical analysis

SHIKU Hitoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (B)

Institution: Tohoku University

2007 - 2008
The development of methods for evaluating embryo qualitybased on the electrochemical measuring technique

ABE Hiroyuki, HITOSHI Shiku, YOKOO Masaki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

2005 - 2008
The development of accurate quantitative and digital tools for Lifesurveyor

KAMBARA Hideki, TAKEYAMA Haruko, SHIKU Hitoshi, KOBATAKE Eiri, AKIYAMA Yutaka, OKAMURA Yoshiko

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research on Priority Areas

Institution: Tokyo University of Agriculture and Technology

2005 - 2008
積層化マイクロ培養デバイスによる電気化学的遺伝子発現アッセイ

珠玖仁

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 若手研究(B)

Institution: 東北大学

2005 - 2006

More details Close

本研究では、3次元微細化工技術とソフトリソグラフィーの融合により、プラスミドアレイ、細胞アレイ、電極アレイを積層した積層型マイクロ培養デバイスを作製し、一連の遺伝子工学操作をオンチップで行うことを試みた。電気化学測定法の利点であるリアルタイム計測と定量性に着目し、遺伝子-タンパク質発現過程を詳細に追跡した。レポーターとしてべ一タ・ガラクトシダーゼ(βGAL)やアルカリホスファターゼ(AP)を用いた。 1、Maltose Binding Protein (MBP)を標的とし、野生型および変異型MBPを発現ベクターを構築し、あらかじめMBPの下流に融合させたβGALの活性を指標として標的タンパク質の可溶性・不溶性を判定するデバイスを開発した。ウェスタンブロッティングによる発現状態との比較から、電流応答とMBPの可溶性発現量がよく対応していることが示された。最終的に電極-培養ウェルー体型デバイスを作製し、発現過程のリアルタイム計測に成功した。 2、酵母Two-hybrid法を利用し、リガンド依存的な核内ホルモン受容体一転写制御因子の相互作用を電気化学的に評価した。17βエストラジオールをリガンドとする酵母Two-hybridシステムにおいて、リガンド濃度依存的にβGAL由来の電流応答増加を確認した。これにより、タンパク質問相互作用の電気化学的評価が微生物チップ上で可能であることが示された。 3、ウェルアレイデバイスのデザインを改良し、各ウェルに単一微生物を捕捉する条件出しを検討した。単一微生物の培養、遺伝子発現、密閉ウェル内への代謝産物の蓄積に伴う蛍光計測などに成功した。昨年度開発したOn-chip遺伝子導入デバイスにおいても、プラスミドDNAおよびホスト大腸菌濃度を最適化することにより、1コロニー/ウェルが実現可能であることが示された。電極-ウェルー体型デバイスでは、細胞活性計測(酵母〜数細胞レベル)および電場制御に基づく細胞マニピュレーション(単一細胞(大腸菌・酵母)の導入・導出)に成功した。 4、動物細胞培養株を用い、3次元培養デバイス上で細胞の極性・非極性を制御し、その培養速度の違いを呼吸活性に基づく電気化学応答として追跡することに成功した。代謝活性のみならずmRNAおよびタンパク質(サイトカイン)レベルでの活性評価にも成功した。
走査型電気化学/化学発光顕微鏡による光反応/電気化学反応制御とイメージング

末永智一, 濱田文男, 珠玖仁, 安川智之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 特定領域研究

Institution: 東北大学

2005 - 2006

More details Close

走査型電気化学顕微鏡(SECM)は、局所反応化学反応の検出・誘起を可能とするユニークなシステムである。探針-基板間距離および探針サイズをナノメートルオーダーで制御し、空間分解能を向上させるとともに、生体機能表面評価およびデバイス作製・環境センシングに応用した。光ファイバプローブを用いて、カルシウムイオン濃度依存性蛍光試薬を導入した神経様細胞PC12に近接させ、シアフォースシグナルと蛍光強度の同時追跡を実施した。ルミノール水溶液をキャピラリプローブに充填し、電気泳動により極微量のルミノールをプローブ外へ導出することにより、イオン電流と発光シグナルの同時観測を行った。泳動電着法により、酵素(グルコースオキシダーゼほか)修飾金ナノ粒子(直径20nm)アレイ電極の所定の位置への酵素パターニングを行った。酵素活性を触媒電流の増加として電極プローブにて観測できた。ソフトリソグラフィーの一種であるマイクロ・コンタクト・プリンティング法により、ジアフォラーゼ、ペルオキシダーゼ、グルコースオキシダーゼをガラス基板上にパターニングした。原子間力顕微鏡観察より、膜厚はほぼ単一分子層相当であることを確認した。SECMでは、ジアフォラーゼ以外の酵素パターンを単分子層レベルで観測することは困難であった。グルタルアルデヒドなど架橋剤の影響により酵素活性が著しい低下することが確認できた。微細加工技術により作製した細胞チップ上に培養細胞や大腸菌をアレイ化し、遺伝子発現や細胞増殖が良好に行われる環境下で、ベータ・ガラクトシダーゼの発現活性を電気化学的に観測し、薬剤の変異原性、ホルモン受容体結合活性、細胞内シグナル伝達のリアルタイム追跡が可能であることを示した。
Development of a high-resolution scanning electrochemical microscope to characterize micro-bio devices

MATSUE Tomokazu, SHIKU Hitoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2003 - 2005

More details Close

We developed a high-resolution scanning electrochemical microscope (SECM) towards characterization of various micro-bio devices. Our SECM-based multifunctional measuring system provides near-field scanning optical microscopic (NSOM) information by featuring an optical fiber-electrode probe. The size of the electrode-, optical fiber-, or optical fiber-electrode- probe is less than 100nm, which allows us high-resolution images of protein arrays and cellular arrays. The feedback mechanism controlling the probe-sample distance is based on the shearing force, by which high-quality images of rough surfaces with the height differences larger than 10μm and fragile samples such as living cells under physiological conditions were captured. 1, Nano-electrode probes were fabricated with the Ti/P sputtering and the subsequent parylene C-vapor deposition polymerization. The effective electrode radius estimated from the cyclic voltammogram for ferrocyanide has found to be less than 50nm. The optical fiber-electrode probes with the radii less than 100nm were made as the same manner. The optical aperture size was also less than 100 nm which was confirmed from the cross section of the NSOM image of the quantum-dot (QD) particles with tens nm-diameters. 2, The feedback mechanism controlling the probe-sample distance was improved by vertically moving the probe with the width of 〜 100nm to reduce the damage applied to the samples. This feedback mode, defined as "standing approach (STA) mode" (Yamada et al., Anal. Chem., 2005, 77, 1785-179), has allowed to simultaneous electrochemical and topographic images of the axon and the cell body of the single PC-12 cell under physiological conditions for the first time. The STA-mode feedback imaging sometimes works better than the tip-sample regulation for the commercially available AFM. For example, relatively larger polystyrene beads (with 〜 10μm diameter) can be imaged with our STA-mode SECM, whereas not imaged with the conventional AFM instrument. 3, Towards the progress in the construction of the micro-bio devices, various techniques to pattern baiomaterials were attempted. Especially, collagen-gel embedded culture of bacteria and mammalian cells with ultra-small volume (tens to several nL) has been successfully demonstrated on glass or silicon chip to microfabricate various types of cellular arrays. Micro-contact printing (μCP), microfluidics, dip-pen nanolithography (DPN), and negative dielectric phoresis (n-DEP) have been also applied to assemble protein and cellular arrays.
走査型電気化学/化学発光顕微鏡を用いた酵素触媒界面の電気化学/発光イメージング

末永智一, 西澤松彦, 珠玖仁

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 特定領域研究

Institution: 東北大学

2003 - 2004

More details Close

本研究では、自作の走査型電気化学顕微鏡(SECM)に発光・蛍光・トポグラフィー計測系をハイブリッドしたシステムにより、ナノメートルレベルの生体機能表面を評価し、デバイス作製や環境センシングへ展開する.これまでの研究で、探針-試料間距離および探針サイズをナノメートルオーダーで制御することを可能にした.これによりイメージングにおける空間分解能の向上のみならず、局所反応誘起に基づくナノリソグラフィーが期待できる. 1.我々の計測システムでは、シアーフォース(せん断応力)フィードバック距離制御を採用することにより、プローブ-基板間距離を100nm以下に保持することが可能となっている.基板と垂直方向にプローブを振動させる距離制御プログラムに改良し、イメージングの際生体試料に与える付加を大幅に軽減することができた.具体的には、プローブをxy方向の振動に加えz方向にも作動して距離制御を行うことで、試料に衝突させずに高解像度イメージングが可能となった(防衛大学山田弘博士との共同研究). 2.細胞を液中で安定にイメージングすることができた.細胞骨格を蛍光染色した試料の形状・蛍光同時イメージングを検討した.さらに、生細胞をそのままイメージングすることも可能であることを確認した. 3.ガラスキャピラリを細尖化したのち、金スパッタ蒸着膜/パリレン蒸着膜の順でコートした.パリレン絶縁膜は安定性に優れ、電極半径70nmのナノ電極を作製できた.電極型(〜100nm)、光ファイバ型(孔径〜100nm)、および光ファイバ-電極複合型ナノプローブ各種を作製した. 4.デバイス作製技術:プロテインアレイのディメンジョンをナノメートルオーダーにスケールダウンするための要素技術として、ナノ微細加工技術、微粒子配列化技術、極微量サンプルの操作技術が挙げられる.誘電泳動を利用して、微粒子のマニピュレーション、タンパク質-細胞のパターニング技術を確立した.

Show all Show first 5