Details of the Researcher

PHOTO

Seonhwa Lee
Section
Graduate School of Pharmaceutical Sciences
Job title
Associate Professor
Degree

  • 博士（韓国誠信女子大学）

  • 修士（韓国誠信女子大学）

e-Rad No.
60519776

Research History 8

  • 2020/05 - Present
    Tohoku University　Graduate School of Pharmaceutical Sciences　Associate Professor

  • 2008/05 - 2020/04
    Tohoku University　Graduate School of Pharmaceutical Sciences　Assistant Professor

  • 2007/11 - 2008/04
    Tohoku University, Sendai, Japan　Graduate School of Pharmaceutical Sciences　Visiting Scholar

  • 2005/10 - 2007/08
    University of Pennsylvania School of Medicine　Center of Excellence in Environmental Toxicology　Director of biomarker facility core

  • 2004/07 - 2007/08
    University of Pennsylvania School of Medicine　Department of Pharmacology　Research assistant professor

  • 2001/08 - 2004/06
    University of Pennsylvania School of Medicine　Center for Cancer Pharmacology　Research associate

  • 1999/10 - 2001/07
    University of Pennsylvania School of Medicine, Philadelphia, PA, USA　Center for Cancer Pharmacology　Postdoctoral Research Fellow

  • 1992/10 - 1999/09
    Korea Institute of Science & Technology (KIST), Seoul, Korea　Bioanalysis & Biotransformation Research Center　Research Scientist

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Education 3

  • Sungshin Women's University　Department of Chemistry　Ph. D.

    1994/03 - 1999/02

  • Sungshin Women's University　Department of Chemistry　Master of Science

    1990/09 - 1992/08

  • Sungshin Women's University, Seoul, Korea　College of Natural Science　Department of Chemistry

    1987/03 - 1990/08

Committee Memberships 2

  • PPF2020/2021　Member of executive committee

    2020/02 - 2021/09

  • The 45th Biological Mass Spectrometry Conference　Member of executive committee

    2017/09 - 2018/07

Research Interests 6

  • Oxidative stress

  • Lipid peroxidation

  • Chemical modification

  • Mass spectrometry

  • Proteomics

  • Lipidomics

Research Areas 2

  • Life sciences / Pharmaceuticals - analytical and physicochemistry /

  • Life sciences / Clinical pharmacy /

Awards 4

  1. Award for Young Scientists from Tohoku Branch of The Pharmaceutical Society of Japan

    2012/12/01　Tohoku Branch of The Pharmaceutical Society of Japan

  2. Top Cited Reviews 2008 to 2009 in Journal of Chromatography B

    2011/08　Journal of Chromatography B

  3. RCM Beynon Prize Award

    2005/06　Rapid Communication in Mass Spectrometry　Best paper published in the journal Rapid Communication in Mass Spectrometry in 2003 – 2004

  4. AFLAC Scholar-in-Training Award

    2004/03　American Association for Cancer Research (AACR)

Papers 97

  1. Screening of Chemical Modifications in Human Skin Keratins by Mass Spectrometry-Based Proteomic Analysis via Noninvasive Sampling and On-Tape Digestion Peer-reviewed

    Seon Hwa Lee, Jiei Kawase, Yusuke Hiroshima, Tomoyuki Oe

    Journal of Proteome Research　2020/08/20

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jproteome.0c00406 　

    ISSN： 1535-3893

    eISSN： 1535-3907

  2. Angiotensin II-Induced Oxidative Stress in Human Endothelial Cells: Modification of Cellular Molecules through Lipid Peroxidation Peer-reviewed

    Seon Hwa Lee, Shuhei Fujioka, Ryo Takahashi, Tomoyuki Oe

    Chemical Research in Toxicology　2019/07

    Publisher: American Chemical Society ({ACS})

    DOI： 10.1021/acs.chemrestox.9b00110 　

  3. Inhibition effect of pyridoxamine on lipid hydroperoxide-derived modifications to human serum albumin Peer-reviewed

    Seon Hwa Lee, Atsushi Matsunaga, Tomoyuki Oe

    PLoS ONE　13　(4)　e0196050　2018/04/01

    Publisher: Public Library of Science

    DOI： 10.1371/journal.pone.0196050 　

    ISSN： 1932-6203

  4. Stable isotope labeling by fatty acids in cell culture (SILFAC) coupled with isotope pattern dependent mass spectrometry for global screening of lipid hydroperoxide-mediated protein modifications Peer-reviewed

    Ryo Takahashi, Shuhei Fujioka, Tomoyuki Oe, Seon Hwa Lee

    JOURNAL OF PROTEOMICS　166　101-114　2017/08

    DOI： 10.1016/j.jprot.2017.07.006 　

    ISSN： 1874-3919

    eISSN： 1876-7737

  5. Oxidative stress-mediated N-terminal protein modifications and MS-based approaches for N-terminal proteomics Invited

    Seon Hwa Lee, Tomoyuki Oe

    DRUG METABOLISM AND PHARMACOKINETICS　31　(1)　27-34　2016/02

    DOI： 10.1016/j.dmpk.2015.12.002 　

    ISSN： 1347-4367

    eISSN： 1880-0920

  6. Tyrosine modifications of insulin-degrading enzyme enable favorable control of substrate specificity for both Alzheimer’s disease and type-2 diabetes mellitus

    Yusuke Hatakawa, Yuki Takeuchi, Seon Hwa Lee, Tomoyuki Oe

    Bioorganic Chemistry　153　107916-107916　2024/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.bioorg.2024.107916 　

    ISSN： 0045-2068

  7. Lipid peroxidation-derived modification and its effect on the activity of glutathione peroxidase 1

    Seon Hwa Lee, Kazuyuki Takahashi, Yusuke Hatakawa, Tomoyuki Oe

    Free Radical Biology and Medicine　208　252-259　2023/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.freeradbiomed.2023.08.014 　

    ISSN： 0891-5849

  8. Stable Isotope Dilution LC/ESI-SRM/MS Analysis for Highly Polar Bioactive Dipeptides Found in Fermented Brown Rice Product using a Porous Graphitic Carbon Column

    Sawaka Ono, Yusuke Hatakawa, Seon Hwa Lee, Tomoyuki Oe

    Food Analytical Methods　2023/09/05

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s12161-023-02524-2 　

    ISSN： 1936-9751

    eISSN： 1936-976X

    More details Close

    Abstract In this study, a validated analytical method based on liquid chromatography/electrospray ionization-selected reaction monitoring/mass spectrometry (LC/ESI-SRM/MS) was developed for dipeptides in a functional food, fermented brown rice and rice bran with Aspergillus oryzae (FBRA). The aim of this study was to screen and quantify dipeptides in the water-soluble fraction of FBRA. There are few studies focusing on dipeptides in FBRA, even though FBRA is expected to contain bioactive peptides: FBRA or its aqueous extract has shown variety of biological activities. An anti-hypertensive dipeptide was found in similar rice bran products digested by thermolysin. Dipeptides are the smallest class of peptides, and many show a variety of biological activities. Some bioactive peptides are widely found in fermented foods. However, because dipeptides are generally too polar to be retained on versatile LC columns, such as octadecylsilyl columns, dipeptides have been often overlooked and not quantified because of the analytical difficulties. The LC was performed using a porous graphitic carbon column with the mobile phases of 0.1% formic acid/0.1% trifluoroacetic acid/1% tetrahydrofuran in water and acetonitrile. The stable isotope-labeled internal standards and SRM for dipeptides made it possible to develop a validated method. Five dipeptides were identified and quantified in FBRA in much higher concentrations than related materials (malted brown rice, rice bran, brown rice, and white rice) as follows: Ile-Arg, 82.1 µg/g; Ala-Phe, 27.8 µg/g; Ala-Tyr, 31.6 µg/g; Val-Phe, 46.3 µg/g; and Ile-Tyr, 49.9 µg/g. This method was simple and robust and would be applicable for other functional foods. Graphical Abstract

  9. Lipid hydroperoxide-derived insulin resistance and its inhibition by pyridoxamine in skeletal muscle cells

    Seon Hwa Lee, Mizuki Tsutsui, Atsushi Matsunaga, Tomoyuki Oe

    Toxicological Research　2022/11/29

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s43188-022-00155-z 　

    ISSN： 1976-8257

    eISSN： 2234-2753

  10. Comparative studies for amyloid beta degradation: “Neprilysin vs insulysin”, “monomeric vs aggregate”, and “whole Aβ40 vs its peptide fragments”

    Dai Kato, Yoshiaki Takahashi, Haruto Iwata, Yusuke Hatakawa, Seon Hwa Lee, Tomoyuki Oe

    Biochemistry and Biophysics Reports　30　101268-101268　2022/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbrep.2022.101268 　

    ISSN： 2405-5808

  11. Alternative LC/ESI-MS/MS approach to screen hemoglobin N-terminal modifications

    Go Usuzawa, Seon Hwa Lee, Tomoyuki Oe

    International Journal of Mass Spectrometry　468　116651-116651　2021/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.ijms.2021.116651 　

    ISSN： 1387-3806

  12. Robust analysis of angiotensin peptides in human plasma: Column switching-parallel LC/ESI-SRM/MS without adsorption or enzymatic decomposition

    Shinsaku Suzuki, Takaaki Goto, Seon Hwa Lee, Tomoyuki Oe

    Analytical Biochemistry　114327-114327　2021/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.ab.2021.114327 　

    ISSN： 0003-2697

  13. Carnosine and anserine in chicken can quench toxic acrylamide under cooking conditions: Mass spectrometric studies on adduct formation and characterization Peer-reviewed

    Aoi Takama, Hiroko Matsubara, Seon Hwa Lee, Tomoyuki Oe

    Food Chemistry　333　127480-127480　2020/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.foodchem.2020.127480 　

    ISSN： 0308-8146

  14. LC-MS analyses of N-acetyl-p-benzoquinone imine-adducts of glutathione, cysteine, N-acetylcysteine, and albumin in a plasma sample: A case study from a patient with a rare acetaminophen-induced acute swelling rash. Peer-reviewed

    Ozawa M, Kubo T, Lee SH, Oe T

    The Journal of toxicological sciences　44　(8)　559-563　2019

    DOI： 10.2131/jts.44.559 　

    ISSN： 0388-1350

  15. Imidazole dipeptides can quench toxic 4-oxo-2(E)-nonenal: Molecular mechanism and mass spectrometric characterization of the reaction products Peer-reviewed

    Fumiya Tatsuno, Seon Hwa Lee, Tomoyuki Oe

    Journal of Peptide Science　e3097　2018/08

    Publisher: Wiley

    DOI： 10.1002/psc.3097 　

  16. Biomimetic trapping cocktail to screen reactive metabolites: use of an amino acid and DNA motif mixture as light/heavy isotope pairs differing in mass shift Peer-reviewed

    Shuto Hosaka, Takuto Honda, Seon Hwa Lee, Tomoyuki Oe

    Analytical and Bioanalytical Chemistry　410　(16)　3847-3857　2018/06/01

    Publisher: Springer Verlag

    DOI： 10.1007/s00216-018-1057-z 　

    ISSN： 1618-2650 1618-2642

  17. An LC/ESI-SRM/MS method to screen chemically modified hemoglobin: simultaneous analysis for oxidized, nitrated, lipidated, and glycated sites Peer-reviewed

    Koki Kojima, Seon Hwa Lee, Tomoyuki Oe

    ANALYTICAL AND BIOANALYTICAL CHEMISTRY　408　(19)　5379-5392　2016/07

    DOI： 10.1007/s00216-016-9635-4 　

    ISSN： 1618-2642

    eISSN： 1618-2650

  18. Mass spectrometry data from proteomic analysis of human skin keratins after exposure to UV radiation Peer-reviewed

    Seon Hwa Lee, Keita Matsushima, Kohei Miyamoto, Tomoyuki Oe

    Data in Brief　7　100-106　2016/06/01

    Publisher: Elsevier Inc.

    DOI： 10.1016/j.dib.2016.02.008 　

    ISSN： 2352-3409

  19. UV irradiation-induced methionine oxidation in human skin keratins: Mass spectrometry-based non-invasive proteomic analysis Peer-reviewed

    Seon Hwa Lee, Keita Matsushima, Kohei Miyamoto, Tomoyuki Oe

    JOURNAL OF PROTEOMICS　133　54-65　2016/02

    DOI： 10.1016/j.jprot.2015.11.026 　

    ISSN： 1874-3919

    eISSN： 1876-7737

  20. 【生】ヒト血清アルブミンの化学修飾解析の効率化 LC/MS/MSと差異解析ソフトの利用

    後藤 貴章, 工藤 裕太, 李 宣和, 大江 知行

    分析化学　64　(9)　653-659　2015/09

    Publisher: (公社)日本分析化学会

    ISSN： 0525-1931

  21. Angiotensin II modification by decomposition products of linoleic acid-derived lipid hydroperoxide Peer-reviewed

    Ryo Takahashi, Takaaki Goto, Tomoyuki Oe, Seon Hwa Lee

    CHEMICO-BIOLOGICAL INTERACTIONS　239　87-99　2015/09

    DOI： 10.1016/j.cbi.2015.06.029 　

    ISSN： 0009-2797

    eISSN： 1872-7786

  22. Efficient Strategy for Screening Chemical Modifications on Human Serum Albumin: Use of LC/MS/MS and Differential Analysis Software Peer-reviewed

    Takaaki Goto, Yuta Kudo, Seon Hwa Lee, Tomoyuki Oe

    BUNSEKI KAGAKU　64　(9)　653-659　2015/09

    DOI： 10.2116/bunsekikagaku.64.653 　

    ISSN： 0525-1931

  23. Hydroxyl Radical-Mediated Novel Modification of Peptides: N-Terminal Cyclization through the Formation of alpha-Ketoamide Peer-reviewed

    Seon Hwa Lee, Hyunsook Kyung, Ryo Yokota, Takaaki Goto, Tomoyuki Oe

    CHEMICAL RESEARCH IN TOXICOLOGY　28　(1)　59-70　2015/01

    DOI： 10.1021/tx500332y 　

    ISSN： 0893-228X

    eISSN： 1520-5010

  24. Mass spectrometric characterizations of protein carbonylation: Comparison between three different conditions, oxidation by Cu-II/ascorbic acid, adduction of methyl glyoxal, and adduction of 4-hydroxy-2(E)-nonenal Peer-reviewed

    Kazuki Maeda, Hiroyuki Funatsu, Yuki Miyazawa, Yasushi Misawa, Takaaki Goto, Seon Hwa Lee, Tomoyuki Oe

    INTERNATIONAL JOURNAL OF MASS SPECTROMETRY　373　72-80　2014/11

    DOI： 10.1016/j.ijms.2014.09.012 　

    ISSN： 1387-3806

    eISSN： 1873-2798

  25. Predicted multiple selected reaction monitoring to screen activated drug-mediated modifications on human serum albumin Peer-reviewed

    Fumio Osaki, Takaaki Goto, Seon Hwa Lee, Tomoyuki Oe

    ANALYTICAL BIOCHEMISTRY　449　59-67　2014/03

    DOI： 10.1016/j.ab.2013.12.016 　

    ISSN： 0003-2697

    eISSN： 1096-0309

  26. N-Terminal α-ketoamide peptides: formation and transamination Peer-reviewed

    Lee, S.H, Kyung, H, Yokota, R, Goto, T, Oe, T

    Chemical Research in Toxicology　27　(4)　637-648　2014/02/14

    DOI： 10.1021/tx4004 　

  27. Aldehyde Stress-Mediated Novel Modification of Proteins: Epimerization of the N-Terminal Amino Acid Peer-reviewed

    Ryo Kajita, Takaaki Goto, Seon Hwa Lee, Tomoyuki Oe

    CHEMICAL RESEARCH IN TOXICOLOGY　26　(12)　1926-1936　2013/12

    DOI： 10.1021/tx400354d 　

    ISSN： 0893-228X

    eISSN： 1520-5010

  28. Oxidative Stress-mediated Chemical Modifications to Biomacromolecules: Mechanism and Implication of Modifications to Human Skin Keratins and Angiotensin II Invited

    Seon Hwa Lee

    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN　133　(10)　1055-1063　2013/10

    DOI： 10.1248/yakushi.13-00176 　

    ISSN： 0031-6903

  29. Can Edman degradation be used for quantification? Isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry and the long-term stability of 20 phenylthiohydantoin-amino acids Amino Acid Analysis Peer-reviewed

    Ryo Satoh, Takaaki Goto, Seon Hwa Lee, Tomoyuki Oe

    Analytical and Bioanalytical Chemistry　405　(25)　8001-8010　2013/10

    DOI： 10.1007/s00216-013-6901-6 　

    ISSN： 1618-2642 1618-2650

    eISSN： 1618-2650

  30. Complete amino acid sequencing and immunoaffinity clean-up can facilitate screening of various chemical modifications on human serum albumin Peer-reviewed

    Takaaki Goto, Kazuyuki Murata, Seon Hwa Lee, Tomoyuki Oe

    ANALYTICAL AND BIOANALYTICAL CHEMISTRY　405　(23)　7383-7395　2013/09

    DOI： 10.1007/s00216-013-7146-0 　

    ISSN： 1618-2642

  31. MALDI-TOF/MS-based label-free binding assay for angiotensin II type 1 receptor: Application for novel angiotensin peptides Peer-reviewed

    Seon Hwa Lee, Takuro Masuda, Takaaki Goto, Tomoyuki Oe

    Analytical Biochemistry　437　(1)　10-16　2013/06/01

    DOI： 10.1016/j.ab.2013.01.023 　

    ISSN： 0003-2697 1096-0309

    eISSN： 1096-0309

  32. Chemical modificomics: a novel strategy for efficient biomarker discovery through chemical modifications on a target peptide Peer-reviewed

    Takaaki Goto, Shota Kojima, Shohei Shitamichi, Seon Hwa Lee, Tomoyuki Oe

    ANALYTICAL METHODS　4　(7)　1945-1952　2012/07

    DOI： 10.1039/c2ay05841c 　

    ISSN： 1759-9660

  33. Non-invasive proteomic analysis of human skin keratins: Screening of methionine oxidation in keratins by mass spectrometry Peer-reviewed

    Seon Hwa Lee, Kohei Miyamoto, Takaaki Goto, Tomoyuki Oe

    JOURNAL OF PROTEOMICS　75　(2)　435-449　2011/12

    DOI： 10.1016/j.jprot.2011.08.010 　

    ISSN： 1874-3919

    eISSN： 1876-7737

  34. Mass Spectrometric Characterization of Modifications to Angiotensin II by Lipid Peroxidation Products, 4-Oxo-2(E)-nonenal and 4-Hydroxy-2(E)-nonenal Peer-reviewed

    Seon Hwa Lee, Ryo Takahashi, Takaaki Goto, Tomoyuki Oe

    CHEMICAL RESEARCH IN TOXICOLOGY　23　(11)　1771-1785　2010/11

    DOI： 10.1021/tx100228q 　

    ISSN： 0893-228X

  35. Analysis of epoxyeicosatrienoic acids by chiral liquid chromatography/electron capture atmospheric pressure chemical ionization mass spectrometry using [C-13]-analog internal standards Peer-reviewed

    Clementina Mesaros, Seon Hwa Lee, Ian A. Blair

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY　24　(22)　3237-3247　2010/11

    DOI： 10.1002/rcm.4760 　

    ISSN： 0951-4198

  36. Liquid chromatography-mass spectrometry (LC-MS) of steroid hormone metabolites and its applications Peer-reviewed

    Trevor M. Penning, Seon-Hwa Lee, Yi Jin, Alejandro Gutierrez, Ian A. Blair

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　121　(3-5)　546-555　2010/08

    DOI： 10.1016/j.jsbmb.2010.01.005 　

    ISSN： 0960-0760

  37. A simple and efficient approach to improve protein identification by the peptide mass fingerprinting method: concomitant use of negative ionization Peer-reviewed

    Takao Sanaki, Mao Suzuki, Seon Hwa Lee, Takaaki Goto, Tomoyuki Oe

    ANALYTICAL METHODS　2　(8)　1144-1151　2010/08

    DOI： 10.1039/c0ay00215a 　

    ISSN： 1759-9660

  38. A 13-Oxo-9,10-epoxytridecenoate Phospholipid Analogue of the Genotoxic 4,5-Epoxy-2E-decenal: Detection in Vivo, Chemical Synthesis, and Adduction with DNA Peer-reviewed

    Clementina Mesaros, Bogdan G. Gugiu, Rong Zhou, Seon Hwa Lee, Jaewoo Choi, James Laird, Ian A. Blair, Robert G. Salomon

    CHEMICAL RESEARCH IN TOXICOLOGY　23　(3)　516-527　2010/03

    DOI： 10.1021/tx9002484 　

    ISSN： 0893-228X

  39. Aldo-keto reductase 1C3 expression in MCF-7 cells reveals roles in steroid hormone and prostaglandin metabolism that may explain its over-expression in breast cancer Peer-reviewed

    Michael C. Byrns, Ling Duan, Seon Hwa Lee, Ian A. Blair, Trevor M. Penning

    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY　118　(3)　177-187　2010/02

    DOI： 10.1016/j.jsbmb.2009.12.009 　

    ISSN： 0960-0760

  40. Combining [C-13(6)]-phenylisothiocyanate and the Edman degradation reaction: a possible breakthrough for absolute quantitative proteomics together with protein identification Peer-reviewed

    Tomoyuki Oe, Masamitsu Maekawa, Ryo Satoh, Seon Hwa Lee, Takaaki Goto

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY　24　(2)　173-179　2010/01

    DOI： 10.1002/rcm.4372 　

    ISSN： 0951-4198

  41. Targeted quantitative analysis of eicosanoid lipids in biological samples using liquid chromatography-tandem mass spectrometry Invited

    Clementina Mesaros, Seon Hwa Lee, Ian A. Blair

    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES　877　(26)　2736-2745　2009/09

    DOI： 10.1016/j.jchromb.2009.03.011 　

    ISSN： 1570-0232

    eISSN： 1873-376X

  42. Targeted chiral lipidomics analysis of bioactive eicosanoid lipids in cellular systems Invited

    Seon Hwa Lee, Ian A. Blair

    BMB REPORTS　42　(7)　401-410　2009/07

    ISSN： 1976-6696

  43. 5-Lipoxygenase-mediated Endogenous DNA Damage Peer-reviewed

    Wenying Jian, Seon Hwa Lee, Michelle V. Williams, Ian A. Blair

    JOURNAL OF BIOLOGICAL CHEMISTRY　284　(25)　16799-16807　2009/06

    DOI： 10.1074/jbc.M109.011841 　

    ISSN： 0021-9258

  44. Analysis of 7,8-Dihydro-8-oxo-2 '-deoxyguanosine in Cellular DNA during Oxidative Stress Peer-reviewed

    Dipti Mangal, Daljit Vudathala, Jong-Heum Park, Seon Hwa Lee, Trevor M. Penning, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　22　(5)　788-797　2009/05

    DOI： 10.1021/tx800343c 　

    ISSN： 0893-228X

    eISSN： 1520-5010

  45. Human Cytosolic Hydroxysteroid Dehydrogenases of the Aldo-ketoreductase Superfamily Catalyze Reduction of Conjugated Steroids IMPLICATIONS FOR PHASE I AND PHASE II STEROID HORMONE METABOLISM Peer-reviewed

    Yi Jin, Ling Duan, Seon Hwa Lee, Helenius J. Kloosterboer, Ian A. Blair, Trevor M. Penning

    JOURNAL OF BIOLOGICAL CHEMISTRY　284　(15)　10013-10022　2009/04

    DOI： 10.1074/jbc.M809465200 　

    ISSN： 0021-9258

  46. Mass spectrometric and computational analysis of cytokine-induced alterations in the astrocyte secretome Peer-reviewed

    Sarah Dunn Keene, Todd M. Greco, Ioannis Parastatidis, Seon-Hwa Lee, Ethan G. Hughes, Rita J. Balice-Gordon, David W. Speicher, Harry Ischiropoulos

    PROTEOMICS　9　(3)　768-782　2009/02

    DOI： 10.1002/pmic.200800385 　

    ISSN： 1615-9853

  47. A Novel 4-Oxo-2(E)-nonenal-Derived Modification to Angiotensin II: Oxidative Decarboxylation of N-Terminal Aspartic Acid Peer-reviewed

    Seon Hwa Lee, Takaaki Goto, Tomoyuki Oe

    CHEMICAL RESEARCH IN TOXICOLOGY　21　(12)　2237-2244　2008/12

    DOI： 10.1021/tx800316v 　

    ISSN： 0893-228X

  48. Cyclooxygenase-2-mediated metabolism of arachidonic acid to 15-oxo-eicosatetraenoic acid by rat intestinal epithelial cells Invited Peer-reviewed

    Seon Hwa Lee, Kannan Rangiah, Michelle V. Williams, Angela Y. Wehr, Raymond N. DuBois, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　20　(11)　1665-1675　2007/11

    DOI： 10.1021/tx700130p 　

    ISSN： 0893-228X

  49. A novel 4-oxo-2(E)-nonenal-derived endogenous thiadiazabicyclo glutathione adduct formed during cellular oxidative stress Peer-reviewed

    Wenying Jian, Seon Hwa Lee, Clementina Mesaros, Tomoyuki Oe, Maria V. Silva Elipe, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　20　(7)　1008-1018　2007/07

    DOI： 10.1021/tx700001t 　

    ISSN： 0893-228X

  50. Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry Peer-reviewed

    Caterina Pettinella, Seon Hwa Lee, Francesco Cipollone, Ian A. Blair

    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES　850　(1-2)　168-176　2007/05

    DOI： 10.1016/j.jchromb.2006.11.023 　

    ISSN： 1570-0232

  51. Targeted chiral lipidomics analysis by liquid chromatography electron capture atmospheric pressure chemical ionization mass spectrometry (LC-ECAPCI/MS) Invited Peer-reviewed

    Seon Hwa Lee, Ian A. Blair

    Methods in Enzymology　433　159-174　2007

    DOI： 10.1016/S0076-6879(07)33009-7 　

    ISSN： 0076-6879

  52. LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells Invited Peer-reviewed

    Seon Hwa Lee, Caterina Pettinella, Ian A. Blair

    CURRENT DRUG METABOLISM　7　(8)　929-937　2006/12

    DOI： 10.2174/138920006779010584 　

    ISSN： 1389-2002

  53. Characterization of a lipid hydroperoxide-derived RNA adduct in rat intestinal epithelial cells Peer-reviewed

    P Zhu, SH Lee, S Wehrli, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　19　(6)　809-817　2006/06

    DOI： 10.1021/tx0600189 　

    ISSN： 0893-228X

  54. Endogenous lipid hydroperoxide-mediated DNA-adduct formation in min mice Peer-reviewed

    MV Williams, SH Lee, M Pollack, IA Blair

    JOURNAL OF BIOLOGICAL CHEMISTRY　281　(15)　10127-10133　2006/04

    DOI： 10.1074/jbc.M600178200 　

    ISSN： 0021-9258

  55. Tibolone metabolism in human liver is catalyzed by 3 alpha/3 beta-hydroxysteroid dehydrogenase activities of the four isoforms of the aldo-keto reductase (AKR)1C subfamily Peer-reviewed

    S Steckelbroeck, B Oyesanmi, Y Jin, SH Lee, HJ Kloosterboer, TM Penning

    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS　316　(3)　1300-1309　2006/03

    DOI： 10.1124/jpet.105.091587 　

    ISSN： 0022-3565

  56. Targeted chiral lipidomics analysis Invited

    SH Lee, MV Williams, IA Blair

    PROSTAGLANDINS & OTHER LIPID MEDIATORS　77　(1-4)　141-157　2005/09

    DOI： 10.1016/j.prostaglandins.2004.01.009 　

    ISSN： 1098-8823

  57. Cyclooxygenase-2-mediated DNA damage Peer-reviewed

    SH Lee, MV Williams, RN DuBois, IA Blair

    JOURNAL OF BIOLOGICAL CHEMISTRY　280　(31)　28337-28346　2005/08

    DOI： 10.1074/jbc.M504178200 　

    ISSN： 0021-9258

  58. Analysis of Fe(II)-mediated decomposition of a linoleic acid-derived lipid hydroperoxide by liquid chromatography/mass spectrometry Peer-reviewed

    SH Lee, T Oe, JS Arora, IA Blair

    JOURNAL OF MASS SPECTROMETRY　40　(5)　661-668　2005/05

    DOI： 10.1002/jms.838 　

    ISSN： 1076-5174

  59. 4-hydroperoxy-2-nonenal-induced formation of 1,N(2)-etheno-2 '-deoxyguanosine adducts Peer-reviewed

    SH Lee, JA Arora, T Oe, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　18　(4)　780-786　2005/04

    DOI： 10.1021/tx0497088 　

    ISSN： 0893-228X

  60. Unexpected formation of etheno-2 '-deoxyguano sine adducts from 5(S)-hydroperoxyeicosatetraenoic acid: Evidence for a bis-hydroperoxide intermediate Peer-reviewed

    WY Jian, SH Lee, JS Arora, MVS Elipe, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　18　(3)　599-610　2005/03

    DOI： 10.1021/tx049693d 　

    ISSN： 0893-228X

  61. Dioxododecenoic acid: A lipid hydroperoxide-derived bifunctional electrophile responsible for etheno DNA adduct formation Peer-reviewed

    SH Lee, MVS Elipe, JS Arora, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　18　(3)　566-578　2005/03

    DOI： 10.1021/tx049716o 　

    ISSN： 0893-228X

  62. Liquid chromatography/mass spectrometry analysis of bifunctional electrophiles and DNA adducts from vitamin C mediated decomposition of 15-hydroperoxyeicosatetraenoic acid Peer-reviewed

    MV Williams, SH Lee, IA Blair

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY　19　(6)　849-858　2005

    DOI： 10.1002/rcm.1854 　

    ISSN： 0951-4198

  63. Viniferin formation by COX-1: Evidence for radical intermediates during co-oxidation of resveratrol Peer-reviewed

    LM Szewczuk, SH Lee, IA Blair, TM Penning

    JOURNAL OF NATURAL PRODUCTS　68　(1)　36-42　2005/01

    DOI： 10.1021/np049702i 　

    ISSN： 0163-3864

  64. Analysis of etheno 2 '-deoxyguanosine adducts as dosimeters of aldo-keto reductase mediated-oxidative stress Peer-reviewed

    SH Lee, IA Blair

    ALDO-KETO REDUCTASES AND TOXICANT METABOLISM　865　139-152　2004

    ISSN： 0097-6156

  65. Chemistry of polycyclic aromatic hydrocarbons (PAH) o-quinones generated by the aldo-keto reductase pathway of PAH activation Peer-reviewed

    Gopishetty, SR, RG Harvey, SH Lee, IA Blair, TM Penning

    ALDO-KETO REDUCTASES AND TOXICANT METABOLISM　865　127-137　2004

    ISSN： 0097-6156

  66. Aldo-keto reductases and the metabolic activation of polycyclic aromatic hydrocarbons Peer-reviewed

    TM Penning, NT Palackal, SH Lee, Blair, I, DS Yu, JA Berlin, JM Field, RG Harvey

    ALDO-KETO REDUCTASES AND TOXICANT METABOLISM　865　83-99　2004

    ISSN： 0097-6156

  67. A novel lipid hydroperoxide-derived modification to arginine Peer-reviewed

    T Oe, SH Lee, MVS Elipe, BH Arison, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　16　(12)　1598-1605　2003/12

    DOI： 10.1021/tx034178l 　

    ISSN： 0893-228X

  68. Altered urinary profiles of polyamines and endogenous steroids in patients with benign cervical disease and cervical cancer Peer-reviewed

    SH Lee, YJ Yang, KM Kim, BC Chung

    CANCER LETTERS　201　(2)　121-131　2003/11

    DOI： 10.1016/S0304-3835(03)00014-4 　

    ISSN： 0304-3835

  69. A novel lipid hydroperoxide-derived cyclic covalent modification to histone H4 Peer-reviewed

    T Oe, JS Arora, SH Lee, IA Blair

    JOURNAL OF BIOLOGICAL CHEMISTRY　278　(43)　42098-42105　2003/10

    DOI： 10.1074/jbc.M308167200 　

    ISSN： 0021-9258

  70. Characterization of 2 '-deoxycytidine adducts derived from 4-oxo-2-nonenal, a novel lipid peroxidation product Peer-reviewed

    M Pollack, T Oe, SH Lee, MVS Elipe, BH Arison, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　16　(7)　893-900　2003/07

    DOI： 10.1021/tx030009p 　

    ISSN： 0893-228X

  71. Targeted lipidomics using electron capture atmospheric pressure chemical ionization mass spectrometry Peer-reviewed

    SH Lee, MV Williams, RN DuBois, IA Blair

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY　17　(19)　2168-2176　2003

    DOI： 10.1002/rcm.1170 　

    ISSN： 0951-4198

    eISSN： 1097-0231

  72. Estrogens in female thyroid cancer: alteration of urinary profiles in pre- and post-operative cases Peer-reviewed

    SH Lee, KM Kim, BH Jung, WY Chung, CS Park, BC Chung

    CANCER LETTERS　189　(1)　27-32　2003/01

    DOI： 10.1016/S0304-3835(02)00514-1 　

    ISSN： 0304-3835

  73. Activation of polycyclic aromatic hydrocarbon trans-dihydrodiol proximate carcinogens by human aldo-keto reductase (AKR1C) enzymes and their functional overexpression in human lung carcinoma (A549) cells Peer-reviewed

    NT Palackal, SH Lee, RG Harvey, IA Blair, TM Penning

    JOURNAL OF BIOLOGICAL CHEMISTRY　277　(27)　24799-24808　2002/07

    DOI： 10.1074/jbc.M112424200 　

    ISSN： 0021-9258

  74. 4,5-Epoxy-2(E)-decenal-induced formation of 1,N-6-etheno-2 '-deoxyadenosine and 1,N-2-etheno-2 '-deoxyguanosine adducts Peer-reviewed

    SH Lee, T Oe, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　15　(3)　300-304　2002/03

    DOI： 10.1021/tx010147j 　

    ISSN： 0893-228X

  75. Vitamin C and cancer Peer-reviewed

    SH Lee, T Oe, IA Blair

    ANTIOXIDANT VITAMINS C AND E　50-65　2002

  76. Human AKR1C isoforms oxidize the potent proximate carcinogen 7,12-DMBA-3,4-diol in the human lung A549 carcinoma cell line Peer-reviewed

    NT Palackal, SH Lee, RG Harvey, IA Blair, TM Penning

    POLYCYCLIC AROMATIC COMPOUNDS　22　(3-4)　801-810　2002

    DOI： 10.1080/10406630290103951 　

    ISSN： 1040-6638

  77. Vitamin C-induced decomposition of lipid hydroperoxides to endogenous genotoxins Peer-reviewed

    SH Lee, T Oe, IA Blair

    SCIENCE　292　(5524)　2083-2086　2001/06

    DOI： 10.1126/science.1059501 　

    ISSN： 0036-8075

  78. Oxidative DNA damage and cardiovascular disease Invited

    SH Lee, IA Blair

    TRENDS IN CARDIOVASCULAR MEDICINE　11　(3-4)　148-155　2001/04

    DOI： 10.1016/S1050-1738(01)00094-9 　

    ISSN： 1050-1738

  79. Amino acid adducts of PAH o-quinones: model studies with naphthalene-1,2-dione Peer-reviewed

    GR Sridhar, VS Murty, SH Lee, IA Blair, TM Penning

    TETRAHEDRON　57　(2)　407-412　2001/01

    DOI： 10.1016/S0040-4020(00)00954-6 　

    ISSN： 0040-4020

  80. Formation of a substituted 1,N-6-etheno-2 '-deoxyadenosine adduct by lipid hydroperoxide-mediated generation of 4-oxo-2-nonenal Peer-reviewed

    D Rindgen, SH Lee, M Nakajima, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　13　(9)　846-852　2000/09

    DOI： 10.1021/tx0000771 　

    ISSN： 0893-228X

  81. Characterization of 4-oxo-2-nonenal as a novel product of lipid peroxidation Peer-reviewed

    SH Lee, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　13　(8)　698-702　2000/08

    DOI： 10.1021/tx000101a 　

    ISSN： 0893-228X

  82. Characterization of 2 '-deoxyadenosine adducts derived from 4-oxo-2-nonenal, a novel product of lipid peroxidation Peer-reviewed

    SH Lee, D Rindgen, RH Bible, E Hajdu, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　13　(7)　565-574　2000/07

    DOI： 10.1021/tx000057z 　

    ISSN： 0893-228X

  83. Comparison of fatty acid profiles in the serum of patients with prostate cancer and benign prostatic hyperplasia Peer-reviewed

    YJ Yang, SH Lee, SJ Hong, BC Chung

    CLINICAL BIOCHEMISTRY　32　(6)　405-409　1999/08

    DOI： 10.1016/S0009-9120(99)00036-3 　

    ISSN： 0009-9120

  84. Androgen imbalance in premenopausal women with benign breast disease and breast cancer Peer-reviewed

    SH Lee, SO Kim, SW Kwon, BC Chung

    CLINICAL BIOCHEMISTRY　32　(5)　375-380　1999/07

    DOI： 10.1016/S0009-9120(99)00028-4 　

    ISSN： 0009-9120

  85. Olefinic reduction of alpha,beta-unsaturated carbonyl compounds catalyzed by activated nickel Peer-reviewed

    SO Kim, S Rhee, SH Lee

    BULLETIN OF THE KOREAN CHEMICAL SOCIETY　20　(7)　773-774　1999/07

    ISSN： 0253-2964

  86. Profiling of urinary environmental estrogens by gas chromatography/mass spectrometry Peer-reviewed

    Yang, Y.J, Lee, S.H, Chung, B.C

    Analytical Science & Technology　12　265-272　1999

  87. Estrogens and polyamines in breast cancer: their profiles and values in disease staging Peer-reviewed

    SH Lee, SO Kim, HD Lee, BC Chung

    CANCER LETTERS　133　(1)　47-56　1998/11

    DOI： 10.1016/S0304-3835(98)00189-X 　

    ISSN： 0304-3835

  88. Gas chromatographic mass spectrometric determination of urinary oxoacids using O-(2,3,4,5,6-pentafluorobenzyl)oxime trimethylsilyl ester derivatization and cation-exchange chromatography Peer-reviewed

    SH Lee, SO Kim, BC Chung

    JOURNAL OF CHROMATOGRAPHY B　719　(1-2)　1-7　1998/11

    DOI： 10.1016/S0378-4347(98)00388-0 　

    ISSN： 0378-4347

  89. Altered profile of endogeneous steroids in the urine of patients with prolactinoma Peer-reviewed

    SH Lee, SY Nam, BC Chung

    CLINICAL BIOCHEMISTRY　31　(7)　529-535　1998/10

    DOI： 10.1016/S0009-9120(98)00063-0 　

    ISSN： 0009-9120

  90. Simultaneous determination of urinary phytoestrogens and estrogens by gas chromatography/mass spectrometry Peer-reviewed

    Yang, Y.J, Lee, S.H, Chung, B.C

    Analytical Science & Technology　11　(5)　374-385　1998/08

  91. Simultaneous determination of cholesterol, bile acids and sterols in human bile juices and gallstones using GC/MS Peer-reviewed

    Yang, Y.J, Lee, S.H, Kim, H, Chung, B.C

    Journal of Korean Chemical Society　42　(3)　266-276　1998/03

    ISSN： 2234-8530

  92. Polyamine profiles in the urine of patients with leukemia Peer-reviewed

    SH Lee, JW Suh, BC Chung, SO Kim

    CANCER LETTERS　122　(1-2)　1-8　1998/01

    DOI： 10.1016/S0304-3835(97)00399-6 　

    ISSN： 0304-3835

  93. GC-MS determination of organic acids with solvent extraction after cation-exchange chromatography Peer-reviewed

    JW Suh, SH Lee, BC Chung

    CLINICAL CHEMISTRY　43　(12)　2256-2261　1997/12

    ISSN： 0009-9147

  94. Evaluation of endogenous steroids profile after administration of anabolic steroids Peer-reviewed

    Lee, S.H, Choi, M, Kim, T, Chung, B.C

    Journal of Korean Chemical Society　41　(8)　406-413　1997/08

  95. Urinary profiling of endogenous estrogens using GC/MS Peer-reviewed

    Lee, S.H, Yang, Y.J, Kim, T, Paeng, K.-J, Chung, B.C

    Journal of Korean Chemical Society　41　(4)　186-197　1997/04

  96. Urinary polyamine evaluation for effective diagnosis of various cancers Peer-reviewed

    JW Suh, SH Lee, BC Chung, J Park

    JOURNAL OF CHROMATOGRAPHY B　688　(2)　179-186　1997/01

    DOI： 10.1016/S0378-4347(96)00266-6 　

    ISSN： 0378-4347

  97. Comparative free and acetylated polyamine profiles in the urine of normal subject and various cancer patients Peer-reviewed

    Suh, J.W, Lee, S.H, Park, Y.H, Chung, B.C, Park, J

    Analytical Science & Technology　8　(4)　895-900　1995

Show all ︎Show first 5

Misc. 96

  1. マススペクトロメトリーを駆使した生体分子解析によるバイオメディカル研究の新展開 脂質ペルオキシドを介したタンパク質の修飾の細胞培養系における脂肪酸の安定同位体標識(SILFAC)と同位体パターン依存的なスキャンを組み合わせたスクリーニング法(Frontier of biomedical science by biomolecule analysis based on mass spectrometry Screening of lipid hydroperoxide-mediated protein mo

    李 宣和, 高橋 亮, 大江 知行

    日本薬学会年会要旨集　137年会　(1)　258-258　2017/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  2. ヒト内皮細胞中のアンジオテンシンII誘発脂質過酸化のLC/ESI-MS解析

    藤岡 修平, 高橋 亮, 大江 知行, 李 宣和

    日本薬学会年会要旨集　137年会　(2)　246-246　2017/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  3. On-tape消化法を用いるヒト表皮ケラチン上の化学修飾スクリーニング

    川瀬 士瑛, 廣嶋 佑亮, 大江 知行, 李 宣和

    日本薬学会年会要旨集　137年会　(2)　286-286　2017/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  4. N末端ピルビン酸アミド型アンジオテンシンの同位体希釈LC/ESI-SRM/MS分析

    池田 真人, 横田 涼, 大江 知行, 李 宣和

    日本薬学会年会要旨集　137年会　(2)　286-286　2017/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  5. 生体模倣トラッピングカクテル:新規反応性代謝物評価法の開発研究

    保坂周斗, 本多拓人, 李宣和, 大江知行

    日本薬学会年会要旨集(CD-ROM)　137th　(2)　ROMBUNNO.25P‐am07S-246　2017/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  6. 反応性代謝物のin vivoスクリーニングを目的としたアルブミンCys34付加体のLC/ESI‐MS/MS

    小澤真史, 久保貴史, 李宣和, 大江知行

    日本薬学会年会要旨集(CD-ROM)　137th　(2)　ROMBUNNO.27PB‐am033S-287　2017/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  7. 過酸化脂質由来の反応性アルデヒドによる化学修飾アミロイドβの研究

    笹本和之, 李宣和, 大江知行

    日本薬学会年会要旨集(CD-ROM)　137th　(2)　ROMBUNNO.27PB‐am032S-287　2017/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  8. 求電子性薬物代謝物とDNA塩基の反応に関する基礎的研究

    中川智恵, 李宣和, 大江知行, 李宣和, 大江知行

    日本薬学会東北支部大会講演要旨集　56th　57　2017

  9. 副作用マーカー探索を目的とした血清アルブミン‐薬物付加体のLC/ESI‐MS解析

    久保貴史, 小澤真史, 李宣和, 大江知行, 李宣和, 大江知行

    日本薬学会東北支部大会講演要旨集　56th　57　2017

  10. What factors influence chemical conjugation reactions on small antibody? Peer-reviewed

    Shuhei Hattori, Takamitsu Hattori, Hikaru Nakazawa, Teppei Niide, Atsushi Matsunaga, Seon Hwa Lee, Tomoyuki Oe, Mitsuo Umetsu

    NEW BIOTECHNOLOGY　33　S204-S204　2016/07

    DOI： 10.1016/j.nbt.2016.06.1423 　

    ISSN： 1871-6784

    eISSN： 1876-4347

  11. 血清アルブミンのN末端異性化解析:アルデヒドストレスマーカーとしての可能性

    藤本健人, 梶田遼, 後藤貴章, 後藤貴章, 後藤貴章, 李宣和, 李宣和, 大江知行, 大江知行

    日本薬学会東北支部大会講演要旨集　55th　50　2016

  12. 凝集性タンパク質のオリゴマー化と酸化修飾の関連解明を目的とした基礎的検討

    横山瑞樹, 李宣和, 大江知行, 李宣和, 大江知行

    日本薬学会東北支部大会講演要旨集　55th　50　2016

  13. Functionalized Edman-type reagents: Applications to absolute protein quantification. Peer-reviewed

    Ryo Satoh, Takaaki Goto, Seon Hwa Lee, Tomoyuki Oe

    62nd ASMS conference on mass spectrometry and allied topics, WP131, Baltimore, USA (ポスター発表)　2014/06

  14. フェロセンを配したエドマン型試薬: タンパク質・ペプチドへの応用研究

    佐藤涼, 後藤貴章, 李宣和, 大江知行

    第74回分析化学討論会，Y1004，郡山 (ポスター発表)　74th　2014/05

  15. LC/ESI-MSによるタンパク質上の過酸化脂質分解物修飾スクリーニング法:Isotope Data Dependent ScanのHSAを用いた検討

    高橋亮, 李宣和, 後藤貴章, 大江知行

    分析化学討論会講演要旨集　74th　2014

  16. N末端α-ケトアミド型ペプチド:生成機構の解明と高感度質量分析法の開発

    横田涼, 景賢淑, 李宣和, 後藤貴章, 大江知行

    日本薬学会東北支部大会講演要旨集　53rd　2014

  17. 血清アルブミン上の網羅的化学修飾解析を指向したLC/MS手法の開発

    工藤裕太, 後藤貴章, 李宣和, 大江知行

    日本薬学会東北支部大会講演要旨集　53rd　2014

  18. 血清アルブミン上の網羅的化学修飾解析を目的とした高分解能LC/MSによる修飾ペプチド検出法の検討

    後藤貴章, 工藤裕太, 李宣和, 大江知行

    分析化学討論会講演要旨集　74th　2014

  19. 標品不要のタンパク質・ペプチド絶対定量法の開発: エドマン分解反応物の同位体希釈LC-ESI/MS/MS法

    佐藤涼, 後藤貴章, 李宣和, 大江知行

    日本分析化学会東北支部若手交流会、仙台 (ポスター発表)　2013/07

  20. 酸化ストレスによる生体高分子の化学修飾機構 : 表皮ケラチン及び昇圧ペプチド類の修飾解析とその生理的意義

    李 宣和

    薬学雑誌　133　(10)　1055-1063　2013

    Publisher: 日本薬学会

    ISSN： 0031-6903

  21. 酸化修飾α-シヌクレインのオリゴマー化に関する基礎的検討

    近藤旭, 李宣和, 後藤貴章, 大江知行

    日本薬学会東北支部大会講演要旨集　52nd　2013

  22. 表皮ケラチンに対する紫外線の影響評価:LC/MSによる酸化修飾解析

    松島慶太, 李宣和, 後藤貴章, 大江知行

    日本薬学会東北支部大会講演要旨集　52nd　2013

  23. Predicted Selected Reaction Monitoringを用いたヘモグロビン上の化学修飾スクリーニング法の開発

    小島弘貴, 李宣和, 後藤貴章, 大江知行

    日本薬学会東北支部大会講演要旨集　52nd　2013

  24. Non-invasive analysis to monitor skin aging conditions : screening of chemical modifications on keratin proteins by mass spectrometry

    大江 知行, 李 宣和, 後藤 貴章

    Cosmetology : Annual report of cosmetology　20　73-77　2012/09

    Publisher: コスメトロジー研究振興財団

    ISSN： 2188-563X

  25. タンパク質標品を用いない網羅的かつ絶対定量的なプロテオーム解析の試み エドマン分解生成物の同位体希釈LC/MS分析の検討

    佐藤 涼, 後藤 貴章, 李 宣和, 大江 知行

    日本薬学会年会要旨集　132年会　(4)　109-109　2012/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  26. 化学修飾スクリーニングを目的とした化学修飾ペプチドのフラグメンテーション解析

    吉泉玲央奈, 李宣和, 後藤貴章, 大江知行

    日本薬学会東北支部大会講演要旨集　51st　2012

  27. タンパク質の酸化修飾に関する基礎的検討

    前田和輝, 李宣和, 後藤貴章, 大江知行

    日本薬学会東北支部大会講演要旨集　51st　2012

  28. アルデヒドによるN末端アミノ酸異性化とその側鎖構造の及ぼす影響

    梶田遼, 後藤貴章, 李宣和, 大江知行

    日本薬学会年会要旨集　132nd　(4)　2012

    ISSN： 0918-9823

  29. エドマン分解反応と同位体希釈分析法を組合せた絶対定量的プロテオミクスの検討

    佐藤 涼, 前川 正充, 後藤 貴章, 伴野 勧, 李 宣和, 大江 知行

    日本薬学会年会要旨集　131年会　(4)　140-140　2011/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  30. 4-hydroxy-2(E)-nonenalによるアンジオテンシンIIの修飾に関する分子メカニズム(Molecular mechanisms of 4-hydroxy-2(E)-nonenal-derived modifications to angiotensin II)

    高橋 亮, 李 宣和, 伴野 勧, 後藤 貴章, 大江 知行

    日本薬学会年会要旨集　131年会　(4)　140-140　2011/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  31. 脂質過酸化物および糖を介したタンパク質のカルボニル化

    伴野 勧, 船津 啓之, 宮澤 佑貴, 後藤 貴章, 李 宣和, 大江 知行

    日本薬学会年会要旨集　131年会　(4)　141-141　2011/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  32. 血清アルブミン上の非酵素的翻訳後修飾プロファイリング法の検討

    後藤 貴章, 村田 和之, 伴野 勧, 李 宣和, 大江 知行

    日本薬学会年会要旨集　131年会　(4)　147-147　2011/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  33. 血清アルブミンの化学的ストレスによる被修飾部位解析

    村田和之, 後藤貴章, 李宣和, 大江知行

    日本薬学会東北支部大会講演要旨集　50th　2011

  34. 内因性アルデヒドによるN末端アミノ酸異性化の促進機構

    梶田遼, 後藤貴章, 李宣和, 大江知行

    バイオメディカル分析科学シンポジウム講演要旨集　24th　2011

    ISSN： 1347-2364

  35. 非侵襲的サンプリングによる表皮ケラチン分析と酸化修飾解析への応用

    宮本 浩平, 李 宣和, 後藤 貴章, 大江 知行

    日本分析化学会講演要旨集　59年会　179-179　2010/09

    Publisher: (公社)日本分析化学会

  36. 脂質過酸化物由来の4-hydroxy-2(E)-nonenalによるangiotensin IIの化学修飾

    高橋 亮, 李 宣和, 後藤 貴章, 大江 知行

    日本分析化学会講演要旨集　59年会　179-179　2010/09

    Publisher: (公社)日本分析化学会

  37. ELISA用抗体を活用したMALDI-TOF/MSによる生理活性ペプチド迅速定量法の検討

    小野寺 賢, 後藤 貴章, 李 宣和, 大江 知行

    日本分析化学会講演要旨集　59年会　180-180　2010/09

    Publisher: (公社)日本分析化学会

  38. エドマン分解反応とLC/MSによる同位体希釈分析を組合せた絶対定量的プロテオミクスの検討

    佐藤 涼, 前川 正充, 後藤 貴章, 李 宣和, 大江 知行

    日本分析化学会講演要旨集　59年会　180-180　2010/09

    Publisher: (公社)日本分析化学会

  39. 標的タンパク質・ペプチド上の化学修飾解析におけるELISA用抗体の活用

    下道 翔平, 小島 昌太, 後藤 貴章, 李 宣和, 大江 知行

    日本分析化学会講演要旨集　59年会　341-341　2010/09

    Publisher: (公社)日本分析化学会

  40. Oxidative Stress-Mediated DNA Damage on Exposure of Cigarette Smoke

    Dipti Mangal, Angela Y. Wehr, Seon Hwa Lee, Clementina Mesaros, Tilo Grosser, Susanne Fries, Trevor M. Penning, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　21　(12)　2446-2447　2008/12

    ISSN： 0893-228X

  41. Isoprostanes Profiling by Liquid Chromatography Electron-Capture Atmospheric Pressure Chemical Ionization Mass Spectrometry

    Clementina Mesaros, Seon-Hwa Lee, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　21　(12)　2445-2446　2008/12

    ISSN： 0893-228X

  42. Characterization of a novel glutathione adduct from arachidonic acid metabolism

    Kannan Rangiah, Seon Hwa Lee, Angela Y. Wehr, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　20　(12)　2000-2000　2007/12

    ISSN： 0893-228X

  43. Formation of 2 '-deoxyguanosine adducts from 12(S)hydroperoxyeicosatetraenoic acid

    Seon Hwa Lee, Clementina Mesaros, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　20　(12)　2003-2003　2007/12

    ISSN： 0893-228X

  44. Aldo-keto reductase 1C3 regulates prostaglandin signaling in breast cancer

    Michael C. Byrns, Seon Hwa Lee, Ling Duan, Ian A. Blair, Trevor M. Penning

    CHEMICAL RESEARCH IN TOXICOLOGY　20　(12)　1997-1997　2007/12

    ISSN： 0893-228X

  45. Analysis of 8-oxo-2 '-deoxyguanosine in human bronchoalveolar dells by immunoaffinity liquid chromatography/mass spectrometry

    Dipti Mangal, Seon Hwa Lee, Jong Heum Park, Trevor M. Penning, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　20　(12)　1999-1999　2007/12

    ISSN： 0893-228X

  46. Oxidative stress-mediated DNA damage

    Ian A. Blair, Seon Hwa Lee, Dipti Mangal, Jong Heum Park, Trevor M. Penning

    CHEMICAL RESEARCH IN TOXICOLOGY　20　(12)　2014-2014　2007/12

    ISSN： 0893-228X

  47. Analysis of 8-oxo-2 '-deoxyguanosine in human bronchoalveolar dells by immunoaffinity liquid chromatography/mass spectrometry

    Dipti Mangal, Seon Hwa Lee, Jong Heum Park, Trevor M. Penning, Ian A. Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　234　2007/08

    ISSN： 0065-7727

  48. Characterization of a novel glutathione adduct from arachidonic acid metabolism

    Kannan Rangiah, Seon Hwa Lee, Angela Y. Wehr, Ian A. Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　234　2007/08

    ISSN： 0065-7727

  49. Formation of 2 '-deoxyguanosine adducts from 12(S)-hydroperoxyeicosatetraenoic acid

    Seon Hwa Lee, Clementina Mesaros, Ian A. Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　234　2007/08

    ISSN： 0065-7727

  50. Oxidative stress-mediated DNA damage

    Ian A. Blair, Seon Hwa Lee, Dipti Mangal, Jong Heum Park, Trevor M. Penning

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　234　2007/08

    ISSN： 0065-7727

  51. Aldo-keto reductase 1C3 regulates prostaglandin signaling in breast cancer

    Michael C. Byrns, Seon Hwa Lee, Ling Duan, Ian A. Blair, Trevor M. Penning

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　234　2007/08

    ISSN： 0065-7727

  52. Benzo[a]pyrene-mediated DNA adduct formation in bronchoalveolar cells.

    Stacy Lynn Gelhaus, Qian Ruan, Seon Hwa Lee, Trevor M. Penning, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　19　(12)　1682-1682　2006/12

    ISSN： 0893-228X

  53. Oxidative DNA damage in benzo[a]pyrene-7,8-dione-treated human bronchoalveolar cells.

    Dipti Mangal, Seon Hwa Lee, Trevor M. Penning, Ian A. Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　19　(12)　1693-1694　2006/12

    ISSN： 0893-228X

  54. TOXI 29-Benzo[a]pyrene-mediated DNA-adduct formation in bronchoalveolar cells

    Stacy Lynn Gelhaus, Qian Ruan, Seon Hwa Lee, Trevor M. Penning, Ian A. Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　232　2006/09

    ISSN： 0065-7727

  55. TOXI 76-Oxidative DNA damage in benzo[a]pyrene-7,8-dione-treated human bronchoalveolar cells

    Dipti Mangal, Seon Hwa Lee, Trevor M. Penning, Ian A. Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　232　2006/09

    ISSN： 0065-7727

  56. Cyclooxygenase-mediated metabolism of arachidonic acid in rat intestinal epithelial cells.

    SH Lee, MV Williams, RN DuBois, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　18　(12)　1984-1985　2005/12

    ISSN： 0893-228X

  57. Characterization of lipid hydroperoxide-derived protein modifications.

    T Oe, SH Lee, JS Arora, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　18　(12)　1985-1985　2005/12

    ISSN： 0893-228X

  58. Characterization of lipid hydroperoxide-derived protein modifications

    T Oe, SH Lee, JS Arora, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　230　U1870-U1871　2005/08

    ISSN： 0065-7727

  59. Cyclooxygenase-mediated metabolism of arachildonic acid in rat intestinal epithelial cells

    SH Lee, MV Williams, RN DuBois, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　230　U1870-U1870　2005/08

    ISSN： 0065-7727

  60. Lipoxygenase-mediated endogenous DNA damage.

    WY Jian, SH Lee, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　17　(12)　1759-1759　2004/12

    ISSN： 0893-228X

  61. Facile synthesis of bifunctional electrophiles to study the oxidative damage to proteins and DNA.

    JS Arora, T Oe, SH Lee, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　17　(12)　1774-1774　2004/12

    ISSN： 0893-228X

  62. Analysis of conjugated estrogens in plasma by LC/electron capture atmospheric pressure chemical ionization/MS.

    XP Cui, SH Lee, P O'Dwyer, P Moate, R Boston, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　17　(12)　1768-1768　2004/12

    ISSN： 0893-228X

  63. Quantitation of DNA adducts derived from lipid peroxidation.

    M Pollack, WY Jian, SH Lee, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　17　(12)　1778-1779　2004/12

    ISSN： 0893-228X

  64. Reactivity and selectivity of lipid hydroperoxide-derived modifications to peptides.

    T Oe, SH Lee, JS Arora, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　17　(12)　1779-1779　2004/12

    ISSN： 0893-228X

  65. Quantitation of DNA-adducts derived from lipid peroxidation.

    M Pollack, WY Jian, SH Lee, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　228　U390-U390　2004/08

    ISSN： 0065-7727

  66. Lipoxygenase mediated endogenous DNA damage.

    WY Jian, SH Lee, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　228　U375-U375　2004/08

    ISSN： 0065-7727

  67. Facile synthesis of bifunctional electrophiles to study the oxidative damage to proteins and DNA.

    JS Arora, T Oe, SH Lee, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　228　U387-U387　2004/08

    ISSN： 0065-7727

  68. Analysis of conjugated estrogens in plasma by liquid chromatography/electron capture atmospheric pressure chemical ionization/mass spectrometry.

    XP Cui, SH Lee, P O'Dwyer, P Moate, R Boston, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　228　U382-U382　2004/08

    ISSN： 0065-7727

  69. Reactivity and selectivity of lipid hydroperoxide-derived modifications to peptides.

    T Oe, SH Lee, JS Arora, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　228　U391-U391　2004/08

    ISSN： 0065-7727

  70. Cyclooxygenase-mediated DNA damage

    IA Blair, SH Lee, MV Williams

    FASEB JOURNAL　18　(8)　C307-C307　2004/05

    ISSN： 0892-6638

  71. Elucidation of estradiol metabolism in human subjects by LC/MS.

    XP Cui, Y Tian, SH Lee, P O'Dwyer, P Moate, R Boston, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　16　(12)　1677-1677　2003/12

    ISSN： 0893-228X

  72. Preparation of deuterium-labeled 4-oxo-2-nonenals for mechanistic studies of oxidative damage to proteins.

    JS Arora, T Oe, SH Lee, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　16　(12)　1670-1670　2003/12

    ISSN： 0893-228X

  73. Lipid hydroperoxide-mediated protein modifications.

    T Oe, JS Arora, SH Lee, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　16　(12)　1680-1681　2003/12

    ISSN： 0893-228X

  74. Lipid hydroperoxide-derived modifications to the repeat hexamer motif of alpha-synuclein.

    SH Lee, T Oe, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　16　(12)　1680-1680　2003/12

    ISSN： 0893-228X

  75. Preparation of deuterium labeled 4-oxo-2-nonenals for mechanistic studies of oxidative damage to proteins.

    JS Arora, T Oe, SH Lee, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　226　U323-U323　2003/09

    ISSN： 0065-7727

  76. Lipid hydroperoxide-derived modifications to the repeat hexamer motif of alpha-synuclein.

    SH Lee, T Oe, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　226　U331-U331　2003/09

    ISSN： 0065-7727

  77. Lipid hydroperoxide-mediated protein modifications.

    T Oe, JS Arora, SH Lee, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　226　U331-U331　2003/09

    ISSN： 0065-7727

  78. Elucidation of estradiol metabolism in human subjects by LC/MS.

    XP Cui, Y Tian, SH Lee, P O'Dwyer, P Moate, R Boston, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　226　U328-U328　2003/09

    ISSN： 0065-7727

  79. Modification of arginine-containing peptides derived from lipid hydroperoxides.

    T Oe, SH Lee, AM Quinn, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　15　(12)　1666-1666　2002/12

    ISSN： 0893-228X

  80. Chemistry of PAH o-quinones generated by the AKR pathway of PAH activation.

    Gopishetty, SR, RG Harvey, SH Lee, IA Blair, TM Penning

    CHEMICAL RESEARCH IN TOXICOLOGY　15　(12)　1665-1665　2002/12

    ISSN： 0893-228X

  81. LC/APCI/MS analysis of etheno 2 '-deoxyguanosine adducts as a dosimeter for AKR mediated-oxidative stress.

    SH Lee, T Oe, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　15　(12)　1665-1665　2002/12

    ISSN： 0893-228X

  82. Lipid hydroperoxide-mediated covalent modifications to sulfhydryl groups.

    AM Quinn, T Oe, SH Lee, IA Blair

    CHEMICAL RESEARCH IN TOXICOLOGY　15　(12)　1665-1666　2002/12

    ISSN： 0893-228X

  83. Aldo-keto reductases and the metabolic activation of polycyclic aromatic hydrocarbons.

    TM Penning, NT Palackal, SH Lee, IA Blair, DS Yu, JA Berlin, JM Field, RG Harvey

    CHEMICAL RESEARCH IN TOXICOLOGY　15　(12)　1656-1656　2002/12

    ISSN： 0893-228X

  84. Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry

    Seon Hwa Lee, Tomoyuki Oe, Ian A. Blair

    Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics　635-636　2002/12

  85. A novel lipid hydroperoxide-derived modification to arginine-containing peptides

    Tomoyuki Oe, Seon Hwa Lee, Amy M. Quinn, Ian A. Blair

    Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics　225-226　2002/12

  86. Lipid hydroperoxide-derived modifications to cellular macromolecules: From DNA to proteomics

    Ian A. Blair, Tomoyuki Oe, Seon Hwa Lee

    Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics　71-72　2002/12

  87. Lipid hydroperoxide-derived 2′-deoxycytidine adducts

    Michael Pollack, Seon Hwa Lee, Tomoyuki Oe, Ian A. Blair

    Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics　633-634　2002/12

  88. Formation of genotoxic bifunctional electrophiles from arachidonic acid hydroperoxides

    Michelle V. Williams, Seon Hwa Lee, Tomoyuki Oe, Ian A. Blair

    Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics　607-608　2002/12/01

  89. Chemistry of PAH o-quinones generated by the AKR pathway of PAH activation.

    Gopishetty, SR, RG Harvey, SH Lee, IA Blair, TM Penning

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　224　U370-U370　2002/08

    ISSN： 0065-7727

  90. Modification of arginine-containing peptides derived from lipid hydroperoxides.

    T Oe, SH Lee, AM Quinn, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　224　U371-U371　2002/08

    ISSN： 0065-7727

  91. Lipid hydroperoxide-mediated covalent modifications to sulfhydryl groups.

    MA Quinn, T Oe, SH Lee, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　224　U371-U371　2002/08

    ISSN： 0065-7727

  92. LC/APCI/MS analysis of etheno 2 '-deoxyguanosine adducts as a dosimeter for AKR mediated-oxidative stress.

    SH Lee, T Oe, IA Blair

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　224　U370-U370　2002/08

    ISSN： 0065-7727

  93. Aldo-keto reductases and the metabolic activation of polycyclic aromatic hydrocarbons.

    TM Penning, NT Palackal, SH Lee, IA Blair, DS Yu, JA Berlin, JM Field, RG Harvey

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　224　U364-U364　2002/08

    ISSN： 0065-7727

  94. The two faces of vitamin C [2] (multiple letters)

    B. Sardi, D. H. Koobs, D. Bar-Or, Seon Hwa Lee, T. Oe, I. A. Blair

    Science　293　1993-1995　2001/09/14

    ISSN： 0036-8075

  95. The two faces of vitamin C - Response

    SH Lee, T Oe, IA Blair

    SCIENCE　293　(5537)　1995-1995　2001/09

    ISSN： 0036-8075

  96. Oxidative stress and lipid hydroperoxide-mediated DNA damage.

    IA Blair, SH Lee, T Oe

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　222　U289-U289　2001/08

    ISSN： 0065-7727

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Books and Other Publications 5

  1. Lipid peroxide-DNA adducts (Chanter 11) in Chemical Carcinogenesis, Current Cancer Research, pp. 227-244.

    Lee, S.H, Blair, I.A

    Springer Science+Business Media LLC　2011

    ISBN: 9781617379949

  2. Chemistry of polycyclic aromatic hydrocarbons (PAH) o-quinones generated by the aldo-keto reductase pathway of PAH activation (Chapter 9) in Aldo-keto reductases and toxicant metabolism (ACS Symposium Series 865), pp.127-137

    Gopishetty, S.R, Harvey, R.G, Lee, S.H, Blair, I.A, Penning, T

    Oxford University Press　2004

    ISBN: 9780841238466

  3. Aldo-keto reductases and the metabolic activation of polycyclic aromatic hydrocarbons (Chapter 6) in Aldo-keto reductases and toxicant metabolism (ACS Symposium Series 865), pp. 83-99

    Penning, T.M, Palackal, N.T, Lee, S.H, Blair, I.A, Yu, D, Berlin, J.A, Field, J.M, Harvey, R.G

    Oxford University Press　2004

    ISBN: 9780841238466

  4. Analysis of etheno 2’-deoxyguanosine adducts as dosimeters of AKR mediated-oxidative stress (Chapter 10) in Aldo-keto reductases and toxicant metabolism (ACS Symposium Series 865), pp.139-152

    Lee, S.H, Blair, I.A

    Oxford University Press　2004

    ISBN: 9780841238466

  5. Vitamin C and Cancer in The antioxidant Vitamins C and E, pp. 50-65

    Lee, S.H, Oe, T, Blair, I.A

    AOCS Press　2002

    ISBN: 1893997294

Presentations 24

  1. Alternative approach for biomarker discovery through chemical modificomics Invited

    Seon Hwa Lee

    2nd Tohoku University–Sichuan University Pharmaceutical Sciences Collaboration Symposium　2019/09/24

  2. Lipid peroxidation-mediated modifications to biomolecules: mass spectrometry-based mechanistic study and selective screening Invited

    Seon Hwa Lee

    2018/12/18

  3. 基礎講座：生体分子の構造解析・定性の基礎 Invited

    Seon Hwa Lee

    第45回BMSコンファレンス　2018/07/05

  4. Screening of lipid hydroperoxide-mediated protein modification using stable isotope labeling by fatty acids in cell culture (SILFAC) coupled with isotope pattern dependent scan Invited

    Seon Hwa Lee

    日本薬学会 第137年会　2017/03/27

  5. Inhibition of lipid hydroperoxide-derived protein damages by pyridoxamine International-presentation

    Lee, S.H, Matsunaga, A, Kyung, H, Oe, T

    KSMS Summer Conference　2016/08/18

  6. Novel oxidative N-terminal modifications to bioactive peptides Invited

    Seon Hwa Lee, Tomoyuki Oe

    新アミノ酸分析研究会第5回学術講演会　2015/12/07

  7. Oxidative stress-derived formation and transamination of N-terminal α-ketoamide peptides/proteins International-presentation

    Lee, S.H, Kyung, H, Yokota, R, Goto, T, Oe, T

    The 62nd Annual ASMS Conference　2014/06/16

  8. Oxidative modifications to bioactive peptides: formations and biological effects Invited

    Seon Hwa Lee

    日本薬学東北支部、第12回生物化学若手研究者セミナー『酸化ストレスに対する応答機構』　2013/09/07

  9. Oxidative modifications to biomacromolecules: mechanism and implication

    Seon Hwa Lee

    日本薬学東北支部、第34回東北薬学セミナー　2012/12/01

  10. Novel lipid hydroperoxide-derived oxidized angiotensin peptides as potential biomarkers of cardiovascular disease International-presentation Invited

    Seon Hwa Lee

    Sendai Symposium on Analytical Sciences　2012/11/09

  11. Targeted metabolomic analysis of modified angiotensin peptides as potential biomarkers of cardiovascular diseases International-presentation Invited

    Lee, S.H, Takahashi, R, Kojima, S, Suzuki, S, Goto, T. Oe, T

    The 60th Annual ASMS Conference　2012/05/21

  12. Targeted metabolomic approach for cardiovascular diseases International-presentation Invited

    Seon Hwa Lee

    Fall International Convention of the Pharmaceutical Society of Korea　2011/07/09

  13. Novel lipid hydroperoxide-derived oxidized angiotensin peptides as potential modulators of cardiovascular functions International-presentation Invited

    Lee, S.H, Takahashi, R, Goto, T, Oe, T

    The 12th Asian-Pacific Congress of Clinical Biochemistry　2010/10/05

  14. Targeted analysis of oxidized bioactive lipids by chiral liquid chromatography-electron capture atmospheric pressure chemical ionization/mass spectrometry (LC-ECAPCI/MS) Invited

    Seon Hwa Lee

    the 34th Annual Meeting of the Japanese Society for Biomedical Mass Spectrometry　2009/09/11

  15. Targeted chiral lipidomics analysis for the metabolism of arachidonic acid related to cardiovascular disease International-presentation Invited

    Seon Hwa Lee

    The 38th Korean Society of Lipidology and Atherosclerosis Conference　2009/09/04

  16. Novel 4-oxo-2(E)-nonenal-derived post-translational modifications to angiotensin peptides International-presentation

    Lee, S.H, Goto, T, Oe, T

    The 57th Annual ASMS Conference　2009/06/03

  17. 4-Oxo-2(E)-nonenal-derived modifications to cellular macromolecules International-presentation Invited

    Lee, S.H, Oe, T, Blair, I.A

    Lipid peroxidation 2008　2008/10/15

  18. Lipid hydroperoxide-mediated oxidative damage to DNA, GSH and peptides Invited

    Seon Hwa Lee

    The Pharmaceutical Society of Japan, Tohoku area meeting　2008/07/12

  19. A possible implication of oxidative modifications to bioactive peptides in disease mechanism

    Lee, S.H, Goto, T, Oe, T

    35th BMS conference　2008/07/06

  20. Biomarkers of cyclooxygenase-2-mediated oxidative stress: formation of GSH- and DNA-adducts International-presentation Invited

    Seon Hwa Lee

    The Convention of the Pharmaceutical Society of Korea　2007/11/07

  21. Formation of 2`-deoxyguanosine adducts from 12(S)-hydroperoxyeicosatetraenoic acid International-presentation

    Lee, S.H, Mesaros, C, Blair, I.A

    234th ACS National Meeting　2007/08/19

  22. LC/MS analysis of reactive metabolites from cyclooxygenase-2-mediated arachidonic acid metabolism International-presentation

    Lee, S.H, Rangiah, K, Williams, M.V, Wehr, A.Y, DuBois, R.N, Blair, I.A

    The 55th Annual ASMS Conference　2007/06/03

  23. Chiral lipidomics analysis of cyclooxygenase-2-mediated arachidonic acid metabolism International-presentation

    Lee, S.H, Wehr, A.Y, Liu, Y, DuBois, R.N, Blair, I.A

    The 54th Annual ASMS Conference　2006/05/28

  24. COX-derived lipids International-presentation Invited

    Lee, S.H, Williams, M.V, Wehr, A.Y, Liu, Y, Blair, I.A

    The First Workshop on Lipid Mediators and Informatics　2006/04/12

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Research Projects 12

  1. インスリン分解酵素の活性分離に及ぼすチロシン修飾の影響

    幡川 祐資, 大江 知行, 李 宣和

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  2. 表皮ケラチンを用いた非侵襲的環境エクスポゾーム解析法の構築

    大江 知行, 李 宣和, 幡川 祐資

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2021/07/09 - 2023/03/31

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    近年、化学物質の環境濃度のみならず、個々人の暴露（exposure）を解析する『エクスポゾーム』の重要性が示唆されている。主にターゲット分子の長期内部暴露を血中タンパク質付加体として解析されるが、環境濃度のみならず滞在時間・防御・吸収・代謝・排泄などの要因もあり、経時的かつ網羅的解析が望まれる。一方、表皮角質層を構成する疎水性タンパク質『ケラチン』は、外部刺激の防護壁として直接かつ恒常的に暴露されており、『エクスポゾーム』の記録媒体として以下の可能性を有する：①多様な化学修飾を受ける、②疎水性低分子を保持できる、③短周期で置き換わる、④非侵襲的に採取できる。そこで申請者は、従来の長期暴露のターゲット分析から経時的かつノンターゲット解析可能な新規環境エクスポゾーム解析法開発を目的とした。 ボランティア16人の表皮ケラチンの酸化修飾を精査した所、7、8月に向けた増加と梅雨期の減少、被覆部位での抑制が見られた。また、秋期には修飾比率が減少し、各条件間の有意差も見られなくなり、表皮ケラチンのターンオーバーにより酸化修飾は蓄積しないことも確認された。一方、予想に反し、日焼け止めクリームの効果に有意差は見られなかった。この結果は、有効成分の紫外線吸収剤で生じる熱やラジカルによる影響の可能性から、紫外線防御効果を相殺したものと思われる。他の化学修飾に関しては、検出限界に近い修飾比率と個人差により、マーカーとして応用可能な物はなかった。 一方、表皮脂質のin vitro実験では、UVB暴露の結果、セラミドとphosphatidylethanolamine は種類が増加し、UVBが表皮脂質種の構造に影響を及ぼす可能性が示唆された。また二重結合の位置・異性化・酸化など構造の特定までは至らなかった。 更に本手法を毛髪ケラチンにも応用し、パーマ・染色などによる酸化修飾も確認した。

  3. Pyridoxamine could be a candidate drug for Parkinson`s disease: Mechanistic study to reduce dopamine-induced toxicity Competitive

    Offer Organization: Grant-in-Aid for Scientific Research

    2019/04 - 2022/03

  4. Glycation and oxidation on amyloid beta: Molecular mechanisms of the effects on aggregation and protease-resistance

    Oe Tomoyuki, SASAMOTO Kazuyuki, KATO Dai, YOKOYAMA Mizuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    Aggregation of Aβ40 was affected by chemical stresses: promoted by H2O2, but inhibited by MG, 4-hydroxy-2(E)-nonenal, 4-hydroxy-2(E)-nonenal, and methylglyoxal. Major modification sites were also identified as follows: Met32, N-term, His6,13,14, Arg5, Lys16, etc. Non-aggregated Aβ40 was digested by neprilysin（NEP）or insulysin（IDE）for one day incubation. However, aggregated Aβ40 was not digested even by prolonged incubation (7 days). From the reaction mixture between non-aggregated Aβ40 and NEP/IDE, several long peptides (ca. 30) were identified. The major peptides (ca. 10 amino acids) were found to include the turn region (E22DVGS26), suggesting that NEP/IDE can recognize the secondary structure, not primary structure.

  5. 内因性ピリドキサミン-アルデヒド付加体の探索：酸化ストレスマーカーとしての評価 Competitive

    System: Grant-in-Aid for Scientific Research

    2016/04 - 2019/03

  6. Use of abundant proteins as molecular recording media: Exhaustive screening strategies for chemical modifications

    OE Tomoyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2017/03/31

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    Proteins are exposed to higher physiological/chemical stresses in some biological events. The resulting chemical modifications on proteins (such as oxidation, glycation, and lipidation) can provide meaningful information about the biological events. Therefore, abundant proteins (such as albumin, hemoglobin, and keratin), that are major targets of chemical modifications,can work as molecular medical record. From the view point, following results have been obtained: i) Chemical modifications on albumin has been screened and found the difference between individuals, ii) A sensitive and simultaneous analytical method has been developed for oxidized, nitrated, lipidated, and glycated hemoglobin, iii) A sensitive and non-invasive analytical method has been developed to estimate UV irradiation-induced skin damage through keratin oxidation.

  7. 神経ペプチド上の過酸化脂質由来の化学修飾解析:神経変性疾患への新規アプローチ Competitive

    System: Grant-in-Aid for Scientific Research

    2013/04 - 2016/03

  8. Development of biomimetic cocktail reagent to screen potential risks of drugs

    OE Tomoyuki, GOTO Takaaki, LEE Seon Hwa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    Background・Aim: Reactive metabolites of drugs result in adverse drug reactions through the formation of covalent biding to biological macromolecules. Therefore, trapping assays, such as glutathione trapping method, are widely performed together with LC-MS for the screening. However, the current strategy still has problems: ex. low throughput, structural gap between trapping reagents and biological macromolecules, and metabolic degradation of the reagents. We therefore aimed to develop a high-throughput/hybrid screening system to solve the problems. Outcome: I) Using simple motif of each nucleophilic site followed by mixing as a cocktail, the structural gap and metabolic degradation were avoided. II) Concomitant use of the stable isotope labeled motifs facilitated the screening because of the characteristic doublet peaks. III) Isotope pattern dependent scanning enabled the automated MS/MS analysis using the characteristic doublet peaks.

  9. Development of a method to screen MS-silent post-translational modifications

    GOTO Takaaki, OE Tomoyuki, LEE Seon Hwa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    A novel methodology to screen MS-silent post-translational modifications on protein has been examined. This method is based on MS-based proteomics combined with the group-specific immunoaffinity extraction, complementary data analyses in both positive and negative mode, and LC/high-resolution MS. This strategy can be used for screening of modifications including isomerization.

  10. Lipid hydroperoxide-derived modifications to angiotensin peptides: a novel approach for cardiovascular disease Competitive

    LEE Seon hwa, OE Tomoyuki, GOTO Takaaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grant-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2010/04 - 2013/03

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    In the reactions of angiotensin (Ang) II with lipid peroxidation-derived bifunctional electrophiles, 4-oxo-2(E)-nonenal (ONE) or 4-hydroxy-2(E)-nonenal (HNE), the major modifications have been identified at the N-terminus, Asp^1, Arg^2, and His^6 of Ang II. The identities of ONE- and HNE-modified Ang IIs were confirmed by mass spectrometry (MS) before and after the reaction with sodium borohydride. A novel ONE-derive pyruvamide-Ang II was formed via oxidative decarboxylation of N-terminal aspartic acid. The unexpected formations of Arg- and His- modifications were confirmed using model reactions with N^α-tert-butoxycarbonyl-amino acids. To investigate the biological effects of modified Ang IIs, MS-based label-free Ang II type 1 (AT_1) receptor binding assay and aminopeptidase (AP) A enzyme assay have been developed. Using these methodologies, we have confirmed that the oxidative modifications on the N-terminus of Ang II disrupt interactions with AT_1 receptor and APA, which could affect the regulation of cardiovascular function.

  11. Why don't you try negative ionization? Alternative challenge to solve inherent problems in proteomics

    OE Tomoyuki, GOTO Takaaki, LEE Seonhwa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

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    Optimization and application of negative ionization MALDI ●MALDI conditions have been optimized for negative ionization. ●Novel peptide mass fingerprinting method has been developed using negative ionization. ●Novel screening method for chemical modifications on HSA has been developed. Optimization and application of negative ionization ESI ● ESI conditions have been optimized for negative ionization ●Fragmentation pattern has been investigated for each chemically modified peptide. ●A screening method for chemical modifications has been developed using the specific fragmentations.

  12. コンフォメーション病の早期診断マーカー探索を指向した挑戦的分析アプローチ

    大江 知行, 後藤 貴章, 李 宣和

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的萌芽研究

    Institution: 東北大学

    2009 - 2011

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    【目的】アルツハイマー病、パーキンソン病、プリオン病に代表されるコンフォメーション病は、原因蛋白質の構造変化、即ち異常構造・凝集体生成に端を発する難病であり、多様な観点から研究されている。 しかし、本疾病の診断・研究は主に、『免疫化学的手法による関連蛋白質の分析』、『分子プローブによる凝集体の検出』を分析基盤として行われ、分別困難な微少な化学修飾の可能性を無視した議論となっている。一方、申請者は、コンフォメーション病のいずれも酸化ストレスと関係が深い事、蛋白質がin vitroで容易に酸化修飾を受ける事に着目し、生体中での酸化修飾が構造変化を惹起する可能性を示唆してきた。本研究では、早期診断マーカー候補の探索を念頭に、従来法では確認が困難であった酸化修飾を分別可能とする精緻な分析基盤の構築を目的とした。 【研究実績】モデルとしてアンジオテンシン類、ケラチン、ヒト血清アルブミン、インシュリンβ鎖等を用いた。各種酸化ストレス条件下、それらの化学修飾反応生成物の同定、反応機構の解明、化学修飾体のクリーンアップ法の開発、質量分析における化学修飾反応生成物のイオン化・フラグメント特性を明らかにすると共に、これらを駆使したスクリーニング法の構築を行った。その結果、生体試料中の微量かつ微小な蛋白質・ペプチドの化学修飾変化を分別し得る分析基盤の確立が示唆された。

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