顔写真

オオツボ ワカコ
大坪 和香子
Wakako Ohtsubo
所属
大学院農学研究科 生物生産科学専攻 動物生命科学講座(動物食品機能学分野)
職名
助教
学位
  • Ph.D.(マールブルク大学)

  • 修士(生命科学)(東北大学)

e-Rad 研究者番号
00598203
プロフィール

よく知られている環境中の、まだ知られていない微生物の多様性や機能に興味があります。

研究室HP:https://www.agri.tohoku.ac.jp/lacto/

https://tohokuagri.wixsite.com/lapc

経歴 5

  • 2015年4月 ~ 継続中
    東北大学大学院農学研究科 助教

  • 2018年9月 ~ 2019年6月
    ワーゲニンゲン大学(オランダ)動物科学研究科 客員研究員

  • 2009年12月 ~ 2015年3月
    東北学院大学 工学総合研究所 研究員

  • 2008年9月 ~ 2008年11月
    東北大学大学院生命科学研究科 博士研究員

  • 2004年5月 ~ 2008年8月
    マックスプランク陸生微生物学研究所

学歴 4

  • マールブルク大学 Philipps-University Marburg

    ~ 2007年12月

  • マックスプランク国際研究大学院 (IMPRS-Mic)

    ~ 2007年12月

  • 東北大学大学院 生命科学研究科 博士課程前期

    ~ 2004年3月

  • 東北大学 農学部

    ~ 2002年3月

委員歴 3

  • Animals Special Issue Editor

    2020年 ~ 2021年

  • Frontiers in Microbiology Topic Editor

    2020年 ~ 2021年

  • 日本無菌生物ノートバイオロジー学会 評議員

    2019年 ~

所属学協会 8

  • 日本農芸化学会

  • 東北畜産学会

  • 日本生物工学会

  • 日本無菌生物ノートバイオロジー学会

  • 日本乳酸菌学会

  • 日本酪農科学会

  • 日本畜産学会

  • 日本微生物生態学会

︎全件表示 ︎最初の5件までを表示

研究キーワード 14

  • 農芸化学

  • 微生物群集構造

  • 動物科学

  • 腸内細菌

  • 機能性食品

  • メタゲノム

  • 嫌気性細菌

  • プレバイオティクス

  • プロバイオティクス

  • 食品微生物学

  • 腸内細菌叢

  • 環境微生物工学

  • 微生物生態学

  • 応用微生物学

研究分野 6

  • ライフサイエンス / 細菌学 /

  • ライフサイエンス / 動物生産科学 /

  • ライフサイエンス / 栄養学、健康科学 /

  • ライフサイエンス / 食品科学 / 食品微生物学

  • ライフサイエンス / 応用微生物学 /

  • ライフサイエンス / 生態学、環境学 / 微生物生態学

受賞 4

  1. 2020年度 東北大学優秀女性研究者賞「紫千代萩賞」

    2021年3月 東北大学 腸内細菌の多様性と機能性およびその有効利用の研究

  2. 第33回学術集会 最優秀発表賞

    2020年11月 日本ウマ科学会 同所飼育環境下のウマにおける腸内常在細菌の個体間伝播

  3. 佐々木正五賞

    2017年6月 日本無菌生物ノートバイオロジー学会

  4. 平成28年度先端農学研究奨励賞

    2016年9月12日 農学研究科

論文 53

  1. Intestinal Microbiota and Immune Modulation in Zebrafish by Fucoidan From Okinawa Mozuku (Cladosiphon okamuranus). 国際誌 査読有り

    Wakako Ikeda-Ohtsubo, Adrià López Nadal, Edoardo Zaccaria, Masahiko Iha, Haruki Kitazawa, Michiel Kleerebezem, Sylvia Brugman

    Frontiers in nutrition 7 67-67 2020年

    DOI: 10.3389/fnut.2020.00067  

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    Fucoidan represents fucose-rich sulfated polysaccharides derived from brown seaweeds, which exerts various biological activities applicable for functional foods and therapeutic agents. The objective of the present study was to investigate in vivo effects of fucoidan extracted from Okinawa mozuku (Cladosiphon okamuranus), common edible seaweed in Japan, on immune responses and microbiota composition in zebrafish. We treated larvae and adult zebrafish with Okinawa mozuku (OM) fucoidan by immersion (100 and 500 μg/mL, 3 days) and by feeding (3 weeks), respectively. The effect of OM fucoidan on immune responses in zebrafish larvae was evaluated by live imaging of neutrophils and macrophages as well as quantitative polymerase chain reaction of pro- and anti-inflammatory cytokine genes. Whole microbiota of zebrafish larvae and intestinal microbiota of adult zebrafish treated with OM fucoidan were analyzed by Illumina MiSeq pair-end sequencing of the V3-V4 region of 16S rRNA genes. Fucoidan treatment only slightly affected the composition of the larvae microbiota and the number of neutrophils and macrophages, while pro- and anti-inflammatory cytokine gene expression levels were upregulated in the larvae treated with 500 μg/mL OM fucoidan. In contrast, feeding of OM fucoidan clearly altered the intestinal microbiota composition of adult zebrafish, which was characterized by the emergence and predominance of multiple bacterial operational taxonomic units (OTUs) affiliated with Rhizobiaceae and Comamonadaceae at the expense of E. coli-related Enterobacteriaceae, the dominant OTUs throughout the studied samples. These changes were accompanied by decreased expression levels of pro-inflammatory cytokine il1b in the intestines of the adult zebrafish. Our current study provides the first insights into in vivo modulatory effects of fucoidan on microbiota and immune responses of unchallenged zebrafish, which underscores the potential of fucoidan to play a modulatory role in the diet-microbiota-host interplay.

  2. ブタ腸管マイクロバイオーム研究から考える ブタの抗菌性成長促進剤(AGP)代替法の開発

    大坪 和香子, 北澤 春樹

    東北畜産学会報 68 (1) 1-7 2018年6月

    出版者・発行元:東北畜産学会

    ISSN:1341-626X

  3. How Can We Define "Optimal Microbiota?": A Comparative Review of Structure and Functions of Microbiota of Animals, Fish, and Plants in Agriculture. 国際誌 査読有り

    Wakako Ikeda-Ohtsubo, Sylvia Brugman, Craig H Warden, Johanna M J Rebel, Gert Folkerts, Corné M J Pieterse

    Frontiers in nutrition 5 90-90 2018年

    出版者・発行元:Frontiers Media {SA}

    DOI: 10.3389/fnut.2018.00090  

    ISSN:2296-861X

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    All multicellular organisms benefit from their own microbiota, which play important roles in maintaining the host nutritional health and immunity. Recently, the number of studies on the microbiota of animals, fish, and plants of economic importance is rapidly expanding and there are increasing expectations that productivity and sustainability in agricultural management can be improved by microbiota manipulation. However, optimizing microbiota is still a challenging task because of the lack of knowledge on the dominant microorganisms or significant variations between microbiota, reflecting sampling biases, different agricultural management as well as breeding backgrounds. To offer a more generalized view on microbiota in agriculture, which can be used for defining criteria of "optimal microbiota" as the goal of manipulation, we summarize here current knowledge on microbiota on animals, fish, and plants with emphasis on bacterial community structure and metabolic functions, and how microbiota can be affected by domestication, conventional agricultural practices, and use of antimicrobial agents. Finally, we discuss future tasks for defining "optimal microbiota," which can improve host growth, nutrition, and immunity and reduce the use of antimicrobial agents in agriculture.

  4. 'Candidatus Adiutrix intracellularis', an endosymbiont of termite gut flagellates, is the first representative of a deep-branching clade of Deltaproteobacteria and a putative homoacetogen. 国際誌 査読有り

    Wakako Ikeda-Ohtsubo, Jürgen F H Strassert, Tim Köhler, Aram Mikaelyan, Ivan Gregor, Alice C McHardy, Susannah Green Tringe, Phil Hugenholtz, Renate Radek, Andreas Brune

    Environmental microbiology 18 (8) 2548-64 2016年9月

    出版者・発行元:Wiley

    DOI: 10.1111/1462-2920.13234  

    ISSN:1462-2912

    詳細を見る 詳細を閉じる

    Termite gut flagellates are typically colonized by specific bacterial symbionts. Here we describe the phylogeny, ultrastructure and subcellular location of 'Candidatus Adiutrix intracellularis', an intracellular symbiont of Trichonympha collaris in the termite Zootermopsis nevadensis. It represents a novel, deep-branching clade of uncultured Deltaproteobacteria widely distributed in intestinal tracts of termites and cockroaches. Fluorescence in situ hybridization and transmission electron microscopy localized the endosymbiont near hydrogenosomes in the posterior part and near the ectosymbiont 'Candidatus Desulfovibrio trichonymphae' in the anterior part of the host cell. The draft genome of 'Ca. Adiutrix intracellularis' obtained from a metagenomic library revealed the presence of a complete gene set encoding the Wood-Ljungdahl pathway, including two homologs of fdhF encoding hydrogenase-linked formate dehydrogenases (FDHH ) and all other components of the recently described hydrogen-dependent carbon dioxide reductase (HDCR) complex, which substantiates previous claims that the symbiont is capable of reductive acetogenesis from CO2 and H2 . The close phylogenetic relationship between the HDCR components and their homologs in homoacetogenic Firmicutes and Spirochaetes suggests that the deltaproteobacterium acquired the capacity for homoacetogenesis via lateral gene transfer. The presence of genes for nitrogen fixation and the biosynthesis of amino acids and cofactors indicate the nutritional nature of the symbiosis.

  5. Neural control of redox response and microbiota-triggered inflammation in Drosophila gut

    Naoyuki Fuse, Haruka Hashiba, Kentaro Ishibashi, Takuro Suzuki, Quang-Dat Nguyen, Kiho Fujii, Wakako Ikeda-Ohtsubo, Haruki Kitazawa, Hiromu Tanimoto, Shoichiro Kurata

    Frontiers in Immunology 14 2023年10月26日

    出版者・発行元:Frontiers Media SA

    DOI: 10.3389/fimmu.2023.1268611  

    eISSN:1664-3224

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    Background The neural system plays a critical role in controlling gut immunity, and the gut microbiota contributes to this process. However, the roles and mechanisms of gut-brain-microbiota interactions remain unclear. To address this issue, we employed Drosophila as a model organism. We have previously shown that NP3253 neurons, which are connected to the brain and gut, are essential for resistance to oral bacterial infections. Here, we aimed to investigate the role of NP3253 neurons in the regulation of gut immunity. Methods We performed RNA-seq analysis of the adult Drosophila gut after genetically inactivating the NP3253 neurons. Flies were reared under oral bacterial infection and normal feeding conditions. In addition, we prepared samples under germ-free conditions to evaluate the role of the microbiota in gut gene expression. We knocked down the genes regulated by NP3253 neurons and examined their susceptibility to oral bacterial infections. Results We found that immune-related gene expression was upregulated in NP3253 neuron-inactivated flies compared to the control. However, this upregulation was abolished in axenic flies, suggesting that the immune response was abnormally activated by the microbiota in NP3253 neuron-inactivated flies. In addition, redox-related gene expression was downregulated in NP3253 neuron-inactivated flies, and this downregulation was also observed in axenic flies. Certain redox-related genes were required for resistance to oral bacterial infections, suggesting that NP3253 neurons regulate the redox responses for gut immunity in a microbiota-independent manner. Conclusion These results show that NP3253 neurons regulate the appropriate gene expression patterns in the gut and contribute to maintain homeostasis during oral infections.

  6. Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium. 国際誌

    Kohtaro Fukuyama, Tao Zhuang, Eita Toyoshi, Fernanda Raya Tonetti, Sudeb Saha, Binghui Zhou, Wakako Ikeda-Ohtsubo, Keita Nishiyama, Hisashi Aso, Julio Villena, Haruki Kitazawa

    Frontiers in immunology 14 1117102-1117102 2023年

    DOI: 10.3389/fimmu.2023.1117102  

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    In vitro culture models that precisely mirror the porcine respiratory epithelium are needed to gain insight into how pathogens and host interact. In this study, a new porcine bronchial epithelial cell line, designated as PBE cells, was established from the respiratory tract of a neonatal pig. PBE cells assumed a cobblestone-epithelial like morphology with close contacts between the cells when they reached confluence. The PBE cell line was characterized in terms of its expression of pattern recognition receptors (PRRs) and its ability to respond to the activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways, which are key PRRs involved in the defense of the respiratory epithelium against pathogens. PBE cells stimulated with poly(I:C) were able to up-regulate the expression of IFN-β, IFN-λ1 (IL-29), IFN-λ3 (IL-28B), the antiviral factors Mx1, OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kinetics studies of immune factors in PBE cells allow us to speculate that this cell line can be a useful in vitro tool to investigate treatments that help to potentiate antiviral immunity in the respiratory epithelium of the porcine host. In addition, poly(I:C) and LPS treatments increased the expression of the inflammatory cytokines TNF-α, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression of negative regulators of the TLR signaling pathways. Then, PBE cells may also allow the evaluation of treatments that can regulate TLR3- and TLR4-mediated inflammatory injury in the porcine airway, thereby protecting the host against harmful overresponses.

  7. The Mucus Binding Factor Is Not Necessary for Lacticaseibacillus rhamnosus CRL1505 to Exert Its Immunomodulatory Activities in Local and Distal Mucosal Sites. 国際誌

    Kae Tomotsune, Fernanda Raya Tonetti, Hiroya Mizuno, Mariano Elean, Kohtaro Fukuyama, Binghui Zhou, Wakako Ikeda-Ohtsubo, Keita Nishiyama, Akihiro Yamamura, Hideaki Karasawa, Shinobu Ohnuma, Akira Horii, Tadao Saito, Haruki Kitazawa, Julio Villena

    International journal of molecular sciences 23 (22) 2022年11月18日

    DOI: 10.3390/ijms232214357  

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    Both viable and non-viable orally administered Lacticaseibacillus rhamnosus CRL1505 modulate immunity in local (intestine) and distal (respiratory) mucosal sites. So, intestinal adhesion and colonization are not necessary for this probiotic strain to exert its immunomodulatory effects. In this work, a mucus-binding factor knockout CRL1505 strain (ΔmbfCRL1505) was obtained and the lack of binding ability to both intestinal epithelial cells and mucin was demonstrated in vitro. In addition, two sets of in vivo experiments in 6-week-old Balb/c mice were performed to evaluate ΔmbfCRL1505 immunomodulatory activities. (A) Orally administered ΔmbfCRL1505 prior to intraperitoneal injection of the Toll-like receptor 3 (TLR3) agonist poly(I:C) significantly reduced intraepithelial lymphocytes (CD3+NK1.1+CD8αα+) and pro-inflammatory mediators (TNF-α, IL-6 and IL-15) in the intestinal mucosa. (B) Orally administered ΔmbfCRL1505 prior to nasal stimulation with poly(I:C) significantly decreased the levels of the biochemical markers of lung tissue damage. In addition, reduced recruitment of neutrophils and levels of pro-inflammatory mediators (TNF-α, IL-6 and IL-8) as well as increased IFN-β and IFN-γ in the respiratory mucosa were observed in ΔmbfCRL1505-treated mice when compared to untreated control mice. The immunological changes induced by the ΔmbfCRL1505 strain were not different from those observed for the wild-type CRL1505 strain. Although it is generally accepted that the expression of adhesion factors is necessary for immunobiotics to induce their beneficial effects, it was demonstrated here that the mbf protein is not required for L. rhamnosus CRL1505 to exert its immunomodulatory activities in local and distal mucosal sites. These results are a step forward towards understanding the mechanisms involved in the immunomodulatory capabilities of L. rhamnosus CRL1505.

  8. Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity 国際誌 国際共著 査読有り

    Mikado Tomokiyo, Fernanda Raya-Tonetti, Hikari Yamamuro, Ryoko Shibata, Kohtaro Fukuyama, Nadia Gobbato, Leonardo Albarracin, Muhammad Shahid Riaz Rajoka, A. K. M. Humayun Kober, Wakako Ikeda-Ohtsubo, Julio Villena, Haruki Kitazawa

    Cells 11 (2986) 1-23 2022年9月25日

    出版者・発行元:MDPI

    DOI: 10.3390/cells11192986  

  9. Soymilk-fermented with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L improves immune-health in pigs. 国際誌

    Y Suda, K Kagawa, K Fukuyama, M Elean, B Zhou, M Tomokiyo, M Aminul Islam, M S R Rajoka, A K M Humayun Kober, T Shimazu, S Egusa, Y Terashima, H Aso, W Ikeda-Ohtsubo, J Villena, H Kitazawa

    Beneficial microbes 1-12 2022年1月31日

    DOI: 10.3920/BM2021.0068  

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    Lactobacillus delbrueckii subsp. delbrueckii TUA4408L has the ability to grow and ferment soymilk and is able to modulate the innate immune response of intestinal epithelial cells in vitro. These two properties prompt us to evaluate whether the soymilk fermented with the TUA4408L strain can induce beneficial immunomodulatory effects in vivo. For this purpose, pigs were selected as a preclinical model. The studies performed here demonstrated that the L. delbrueckii subsp. delbrueckii TUA4408L-fermented soymilk (TUA4408L FSM) reduced blood markers of inflammation and differentially regulated the expression of inflammatory and regulatory cytokines in the intestinal mucosa. These immunological changes induced by the TUA4408L FSM were associated to an enhanced resistance to pathogenic Escherichia coli and an improved grow performance and meat quality of pigs. The experiments and analysis in our study indicate that the immunobiotic TUA4408L FSM could be an interesting non-dairy functional food to beneficially modulate the intestinal immune system, improve protection against pathogens and reduce inflammatory damage. The preclinical study carried out here in pigs could have a better correlation in humans, compared to a rodent model. However, the clinical relevance of these findings still needs to be confirmed by further research, for example, in controlled human challenge studies.

  10. Immunobiotic Feed Developed with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and the Soymilk By-Product Okara Improves Health and Growth Performance in Pigs. 国際誌

    Yoshihito Suda, Nana Sasaki, Kyoma Kagawa, Mariano Elean, Binghui Zhou, Mikado Tomokiyo, Md Aminul Islam, Muhammad Shahid Riaz Rajoka, A K M Humayun Kober, Tomoyuki Shimazu, Shintaro Egusa, Yuji Terashima, Hisashi Aso, Wakako Ikeda-Ohtsubo, Julio Villena, Haruki Kitazawa

    Microorganisms 9 (5) 2021年4月25日

    DOI: 10.3390/microorganisms9050921  

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    Lactobacillus delbrueckii subsp. delbrueckii TUA4408L is able to differentially modulate the innate immune response of porcine intestinal epithelial cells triggered by TLR4 activation. This strain also has a remarkable ability to grow on plant substrates. These two immunological and biotechnological characteristics prompted us to evaluate whether the soymilk by-product okara fermented with the TUA4408L strain can serve as an immunobiotic feed with the ability to beneficially modulate the intestinal immunity of piglets after weaning to improve their productivity. Our in vivo studies demonstrated that the administration of immunobiotic TUA4408L-fermented okara feed significantly increased piglet growth performance and meat quality. These positive effects were associated with the ability of the TUA4408L-fermented okara feed to beneficially modulate both intestinal microbiota and immunity in pigs. The immunobiotic feed improved the abundance of the beneficial bacteria Lactobacillus and Lactococcus in the gut of pigs, reduced blood markers of inflammation, and differentially regulated the expression of inflammatory and regulatory cytokines in the intestinal mucosa. These findings indicate that the immunobiotic TUA4408L-fermented okara feed could be an economical and environmentally friendly option to improve the growth performance and immune health of pigs.

  11. Ligilactobacillus salivarius Strains Isolated From the Porcine Gut Modulate Innate Immune Responses in Epithelial Cells and Improve Protection Against Intestinal Viral-Bacterial Superinfection. 国際誌

    Yuhki Indo, Shugo Kitahara, Mikado Tomokiyo, Shota Araki, Md Aminul Islam, Binghui Zhou, Leonardo Albarracin, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Takato Takenouchi, Hirohide Uenishi, Hisashi Aso, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Frontiers in immunology 12 652923-652923 2021年

    DOI: 10.3389/fimmu.2021.652923  

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    Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of L. salivarius strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: L. salivarius FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic E. coli (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, L. salivarius FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. In vivo studies performed in mice models confirmed the ability of L. salivarius FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets.

  12. Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies. 国際誌

    Binghui Zhou, Leonardo Albarracin, Yuhki Indo, Lorena Arce, Yuki Masumizu, Mikado Tomokiyo, Md Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Hidetoshi Morita, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Microorganisms 8 (11) 2020年10月26日

    DOI: 10.3390/microorganisms8111659  

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    In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different "immune" and "adhesion" phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.

  13. Draft Genome Sequence of Ligilactobacillus salivarius FFIG58, Isolated from the Intestinal Tract of Wakame-Fed Pig. 国際誌

    Binghui Zhou, Leonardo Albarracin, Yuki Masumizu, Yuhki Indo, M Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Julio Villena, Haruki Kitazawa

    Microbiology resource announcements 9 (34) 2020年8月20日

    出版者・発行元:American Society for Microbiology

    DOI: 10.1128/MRA.00839-20  

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    Ligilactobacillus salivarius FFIG58 was isolated from the intestine of a wakame-fed pig and sequenced with an Illumina HiSeq system. FFIG58 genome sequencing revealed a genome size of 1,984,180 bp, with 1,994 protein-coding genes and a GC content of 32.9%. This draft genome sequence will contribute to a better understanding of the porcine gut microbiome.

  14. Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics. 国際誌 査読有り

    Asuka Tada, Akm Humayun Kober, Md Aminul Islam, Manami Igata, Michihiro Takagi, Masahiko Suzuki, Hisashi Aso, Wakako Ikeda-Ohtsubo, Kazutoyo Yoda, Kenji Miyazawa, Fang He, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Cells 9 (7) 2020年7月17日

    DOI: 10.3390/cells9071715  

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    The degree of fat accumulation and adipokine production are two major indicators of obesity that are correlated with increased adipose tissue mass and chronic inflammatory responses. Adipocytes have been considered effector cells for the inflammatory responses due to their capacity to express Toll-like receptors (TLRs). In this study, we evaluated the degree of fat accumulation and adipokine production in porcine intramuscular preadipocyte (PIP) cells maintained for in vitro differentiation over a long period without or with stimulation of either TNF-α or TLR2-, TLR3-, or TLR4-ligands. The cytosolic fat accumulation was measured by liquid chromatography and the expression of adipokines (CCL2, IL-6, IL-8 and IL-10) were quantified by RT-qPCR and ELISA at several time points (0 to 20 days) of PIP cells differentiation. Long-term adipogenic differentiation (LTAD) induced a progressive fat accumulation in the adipocytes over time. Activation of TLR3 and TLR4 resulted in an increased rate of fat accumulation into the adipocytes over the LTAD. The production of CCL2, IL-8 and IL-6 were significantly increased in unstimulated adipocytes during the LTAD, while IL-10 expression remained stable over the studied period. An increasing trend of adiponectin and leptin production was also observed during the LTAD. On the other hand, the stimulation of adipocytes with TLRs agonists or TNF-α resulted in an increasing trend of CCL2, IL-6 and IL-8 production while IL-10 remained stable in all four treatments during the LTAD. We also examined the influences of several immunoregulatory probiotic strains (immunobiotics) on the modulation of the fat accumulation and adipokine production using supernatants of immunobiotic-treated intestinal immune cells and the LTAD of PIP cells. Immunobiotics have shown a strain-specific ability to modulate the fat accumulation and adipokine production, and differentiation of adipocytes. Here, we expanded the utility and potential application of our in vitro PIP cells model by evaluating an LTAD period (20 days) in order to elucidate further insights of chronic inflammatory pathobiology of adipocytes associated with obesity as well as to explore the prospects of immunomodulatory intervention for obesity such as immunobiotics.

  15. Erratum: Fukuyama, K., et al. Evaluation of the Immunomodulatory Ability of Lactic Acid Bacteria Isolated from Feedlot Cattle Against Mastitis Using a Bovine Mammary Epithelial Cells In Vitro Assay. Pathogens 2020, 9, 410. 国際誌 査読有り

    Kohtaro Fukuyama, Md Aminul Islam, Michihiro Takagi, Wakako Ikeda-Ohtsubo, Shoichiro Kurata, Hisashi Aso, Maria Elena Fatima Nader-Macías, Graciela Vignolo, Julio Villena, Haruki Kitazawa

    Pathogens (Basel, Switzerland) 9 (7) 2020年7月16日

    DOI: 10.3390/pathogens9070574  

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    The authors would like to make the following corrections about the published paper [...].

  16. Evaluation of the Immunomodulatory Ability of Lactic Acid Bacteria Isolated from Feedlot Cattle Against Mastitis Using a Bovine Mammary Epithelial Cells In Vitro Assay. 国際誌 査読有り

    Kohtaro Fukuyama, Md Aminul Islam, Michihiro Takagi, Wakako Ikeda-Ohtsubo, Shoichiro Kurata, Hisashi Aso, Graciela Vignolo, Julio Villena, Haruki Kitazawa

    Pathogens (Basel, Switzerland) 9 (5) 2020年5月25日

    DOI: 10.3390/pathogens9050410  

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    Bovine mastitis, the inflammation of the mammary gland, affects the quality and quantity of milk yield. Mastitis control relies on single or multiple combinations of antibiotic therapy. Due to increasing antibiotic resistance in pathogens, the intramammary infusion of lactic acid bacteria (LAB) has been considered as a potential alternative to antibiotics for treating and preventing bovine mastitis through the improvement of the host immunity. Probiotic effects are a strain-dependent characteristic; therefore, candidate LAB strains have to be evaluated efficiently to find out the ones with the best potential. Here, we investigated LAB strains originally isolated from feedlot cattle's environment regarding their ability in inducing the Toll-like receptor (TLR)-triggered inflammatory responses in bovine mammary epithelial (BME) cells in vitro. The BME cells were pre-stimulated with the LAB strains individually for 12, 24, and 48 h and then challenged with Escherichia coli-derived lipopolysaccharide (LPS) for 12 h. The mRNA expression of selected immune genes-interleukin 1 alpha (IL-1α), IL-1β, monocyte chemotactic protein 1 (MCP-1), IL-8, chemokine (C-X-C motif) ligand 2 (CXCL2), and CXCL3 were quantified by real-time quantitative PCR (RT-qPCR). Results indicated that pretreatment with some Lactobacillus strains were able to differentially regulate the LPS inflammatory response in BME cells; however, strain-dependent differences were found. The most remarkable effects were found for Lactobacillus acidophilus CRL2074, which reduced the expression of IL-1α, IL-1β, MCP-1, IL-8, and CXCL3, whereas Lactobacillus rhamnosus CRL2084 diminished IL-1β, MCP-1, and IL-8 expression. The pre-stimulation of BME cells with the CRL2074 strain resulted in the upregulated expression of three negative regulators of the TLRs, including the ubiquitin-editing enzyme A20 (also called tumor necrosis factor alpha-induced protein 3, TNFAIP3), single immunoglobin IL-1 single receptor (SIGIRR), and Toll interacting protein (Tollip) after the LPS challenge. The CRL2084 pre-stimulation upregulated only Tollip expression. Our results demonstrated that the L. acidophilus CRL2074 strain possess remarkable immunomodulatory abilities against LPS-induced inflammation in BME cells. This Lactobacillus strain could be used as candidate for in vivo testing due to its beneficial effects in bovine mastitis through intramammary infusion. Our findings also suggest that the BME cells immunoassay system could be of value for the in vitro evaluation of the immunomodulatory abilities of LAB against the inflammation resulting from the intramammary infection with mastitis-related pathogens.

  17. Transcriptome Analysis of The Inflammatory Responses of Bovine Mammary Epithelial Cells: Exploring Immunomodulatory Target Genes for Bovine Mastitis. 国際誌 査読有り

    Md Aminul Islam, Michihiro Takagi, Kohtaro Fukuyama, Ryoya Komatsu, Leonardo Albarracin, Tomonori Nochi, Yoshihito Suda, Wakako Ikeda-Ohtsubo, Victor Rutten, Willem van Eden, Julio Villena, Hisashi Aso, Haruki Kitazawa

    Pathogens (Basel, Switzerland) 9 (3) 2020年3月9日

    DOI: 10.3390/pathogens9030200  

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    Bovine mastitis is the inflammatory reaction of the mammary gland and is commonly caused by bacterial infections in high-yielding dairy cows. The detailed investigation of the immunotranscriptomic response of bovine mammary epithelial (BME) cells to pattern recognition receptors (PRRs) activation by microbial-associated molecular patterns (MAMPs) can be of great importance for understanding the innate immune defense mechanisms, and for exploring the immunomodulatory candidate genes. In this work, we investigated the transcriptome modifications of BME cells after the in vitro stimulation with Escherichia coli derived lipopolysaccharide (LPS) and heat-killed Staphylococcus aureus JE2 and S. aureus SA003. In addition, the effect of Pam3CSK4 (a synthetic triacylated lipopeptide that activates Toll-like receptor 2 (TLR2)), and the intracellular chemotactic protein cyclophilin A (CyPA), which is secreted by BME cells during mastitis, in the expression changes of selected cytokines and chemokines were evaluated by qPCR. Microarray analysis identified 447, 465 and 520 differentially expressed genes (DEGs) in the BME cells after LPS, S. aureus JE2 and S. aureus SA003 stimulation, respectively. A major differential response in the inflammatory gene expression was noticed between the stimulation of LPS and S. aureus strains. Unlike the S. aureus strains, LPS stimulation resulted in significant upregulation of CCL2, CXCL2, CXCL3, CXCL8, IL1 and IL1, which were confirmed by qPCR analysis. Pam3CSK4 was not able to induce significant changes in the expression of cytokines and chemokines in challenged BME cells. The exogenous CyPA administration was able to upregulate CXCL2, CXCL3, CXCL8, IL1 and IL1 expression in BME cells indicating its ability to promote inflammation. The identification of transcriptional markers of mastitis specific for individual inflammatory factors such as LPS, Pam3CSK4 or CyPA, which can be evaluated in vitro in BME cells, may enable the development of novel diagnostics and/or immunomodulatory treatments, providing new tools for the effective management of mastitis in dairy cows. The results of this work are an advance in this regard.

  18. Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin. 国際誌 査読有り

    Asuka Tada, Md Aminul Islam, Akm Humayun Kober, Kohtaro Fukuyama, Michihiro Takagi, Manami Igata, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Hideki Takahashi, Julio Villena, Hisashi Aso, Haruki Kitazawa

    International journal of molecular sciences 21 (2) 2020年1月18日

    DOI: 10.3390/ijms21020638  

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    Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-α. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-α by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-α stimulation, respectively. Expression changes in APP, HNF4A, ESR1, EGR1, SRC, HNF1A, FN1, ALB, STAT3, CBL, CEBPB, AR, FOS, CFTR, PAN2, PTPN6, VDR, PPARG, STAT5A and NCOA3 genes which are enriched in the 'PPAR signaling' and 'insulin resistance' pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of 'GPCR ligand-binding', 'cell chemotaxis', 'blood coagulation and complement', 'metabolism of lipid and lipoproteins', 'regulation of lipid metabolism by PPARA', and 'lipid digestion, mobilization and transport' pathways. TNF-α stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-α to the pMA of intramuscular origin.

  19. Immunobiotic Lactobacillus jensenii TL2937 Alleviates Dextran Sodium Sulfate-Induced Colitis by Differentially Modulating the Transcriptomic Response of Intestinal Epithelial Cells. 国際誌

    Nana Sato, Valeria Garcia-Castillo, Mao Yuzawa, Md Aminul Islam, Leonardo Albarracin, Mikado Tomokiyo, Wakako Ikeda-Ohtsubo, Apolinaria Garcia-Cancino, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Frontiers in immunology 11 2174-2174 2020年

    DOI: 10.3389/fimmu.2020.02174  

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    Immunobiotics have emerged as a promising intervention to alleviate intestinal damage in inflammatory bowel disease (IBD). However, the beneficial properties of immunobiotics are strain dependent and, therefore, each strain has to be evaluated in order to demonstrate its potential application in IBD. Our previous in vitro and in vivo studies demonstrated that Lactobacillus jensenii TL2937 attenuates gut acute inflammatory response triggered by Toll-like receptor 4 activation. However, its effect on colitis has not been evaluated before. In this work, we studied whether the TL2937 strain was able to protect against the development of colitis in a dextran sodium sulfate (DSS)-induced mouse model and we delved into the mechanisms of action by evaluating the effect of the immunobiotic bacteria on the transcriptomic response of DSS-challenged intestinal epithelial cells. L. jensenii TL2937 was administered to adult BALB/c mice before the induction of colitis by the administration of DSS. Colitis and the associated inflammatory response were evaluated for 14 days. Mice fed with L. jensenii TL2937 had lower disease activity index and alterations of colon length when compared to control mice. Reduced myeloperoxidase activity, lower production of pro-inflammatory (TNF-α, IL-1, CXCL1, MCP-1, IL-15, and IL-17), and higher levels of immunoregulatory (IL-10 and IL-27) cytokines were found in the colon of TL2937-treated mice. In addition, the treatment of porcine intestinal epithelial (PIE) cells with L. jensenii TL2937 before the challenge with DSS differentially regulated the activation of the JNK pathway, leading to an increase in epithelial cell integrity and to a differential immunotranscriptomic response. TL2937-treated PIE cells had a significant reduction in the expression of inflammatory cytokines (TNF-α, IL-1α, IL-1β, IL-6, IL-15), chemokines (CCL2, CCL4, CCL8, CXCL4, CXCL5, CXCL9, CXCL10), adhesion molecules (SELE, SELL, EPCAM), and other immune factors (NCF1, NCF2, NOS2, SAA2) when compared to control cells after the challenge with DSS. The findings of this work indicate that (a) L. jensenii TL2937 is able to alleviate DSS-induced colitis suggesting a potential novel application for this immunobiotic strain, (b) the modulation of the transcriptomic response of intestinal epithelial cells would play a key role in the beneficial effects of the TL2937 strain on colitis, and (c) the in vitro PIE cell immunoassay system could be of value for the screening and selection of new immunobiotic strains for their application in IBD.

  20. Exopolysaccharides From Streptococcus thermophilus ST538 Modulate the Antiviral Innate Immune Response in Porcine Intestinal Epitheliocytes. 国際誌 査読有り

    Hiroya Mizuno, Kae Tomotsune, Md Aminul Islam, Ryutaro Funabashi, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Hisashi Aso, Hideki Takahashi, Katsunori Kimura, Julio Villena, Yasuko Sasaki, Haruki Kitazawa

    Frontiers in microbiology 11 894-894 2020年

    DOI: 10.3389/fmicb.2020.00894  

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    It was reported that exopolysaccharides (EPSs) from lactobacilli are able to differentially modulate mucosal antiviral immunity. Although research has described the ability of EPSs derived from Streptococcus thermophilus to modulate the mucosal immune system, their impact on antiviral immunity was less explored. In this work, we investigated the capacity of the EPS-producing S. thermophilus ST538 to modulate the innate antiviral immune response triggered by the activation of the Toll-like receptor 3 (TLR3) in porcine intestinal epitheliocytes (PIE cells). Moreover, in order to study the immunomodulatory potential of S. thermophilus ST538 EPS, we successfully developed two mutant strains through the knockout of the epsB or epsC genes. High-performance liquid chromatography and scanning electron microscopy studies demonstrated that the wild type (WT) strain produced as high as 595 μg/ml of EPS in the skim milk medium, while none of the mutant strains (S. thermophilus ΔepsB and ΔepsC) were able to produce EPS. Studies in PIE cells demonstrated that the EPS of S. thermophilus ST538 is able to significantly improve the expression of interferon β (IFN-β), interleukin 6 (IL-6), and C-X-C motif chemokine 10 (CXCL10) in response to TLR3 stimulation. The role of EPS in the modulation of antiviral immune response in PIE cells was confirmed by comparative studies of cell free culture supernatants and fermented skim milks obtained from S. thermophilus ΔepsB and ΔepsC. These results suggest that S. thermophilus ST538 could be used as an immunobiotic strain for the development of new immunologically functional foods, which might contribute to improve resistance against viral infections.

  21. Feed, Microbiota, and Gut Immunity: Using the Zebrafish Model to Understand Fish Health. 国際誌 査読有り

    Adrià López Nadal, Wakako Ikeda-Ohtsubo, Detmer Sipkema, David Peggs, Charles McGurk, Maria Forlenza, Geert F Wiegertjes, Sylvia Brugman

    Frontiers in immunology 11 114-114 2020年

    DOI: 10.3389/fimmu.2020.00114  

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    Aquafeed companies aim to provide solutions to the various challenges related to nutrition and health in aquaculture. Solutions to promote feed efficiency and growth, as well as improving the fish health or protect the fish gut from inflammation may include dietary additives such as prebiotics and probiotics. The general assumption is that feed additives can alter the fish microbiota which, in turn, interacts with the host immune system. However, the exact mechanisms by which feed influences host-microbe-immune interactions in fish still remain largely unexplored. Zebrafish rapidly have become a well-recognized animal model to study host-microbe-immune interactions because of the diverse set of research tools available for these small cyprinids. Genome editing technologies can create specific gene-deficient zebrafish that may contribute to our understanding of immune functions. Zebrafish larvae are optically transparent, which allows for in vivo imaging of specific (immune) cell populations in whole transgenic organisms. Germ-free individuals can be reared to study host-microbe interactions. Altogether, these unique zebrafish features may help shed light on the mechanisms by which feed influences host-microbe-immune interactions and ultimately fish health. In this review, we first describe the anatomy and function of the zebrafish gut: the main surface where feed influences host-microbe-immune interactions. Then, we further describe what is currently known about the molecular pathways that underlie this interaction in the zebrafish gut. Finally, we summarize and critically review most of the recent research on prebiotics and probiotics in relation to alterations of zebrafish microbiota and immune responses. We discuss the advantages and disadvantages of the zebrafish as an animal model for other fish species to study feed effects on host-microbe-immune interactions.

  22. Paraimmunobiotic Bifidobacteria Modulate the Expression Patterns of Peptidoglycan Recognition Proteins in Porcine Intestinal Epitheliocytes and Antigen Presenting Cells. 国際誌 査読有り

    Hikaru Iida, Masanori Tohno, Md Aminul Islam, Nana Sato, Hisakazu Kobayashi, Leonardo Albarracin, Akm Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Tomonori Nochi, Ayako Miyazaki, Hirohide Uenishi, Noriyuki Iwabuchi, Jin-Zhong Xiao, Julio Villena, Haruki Kitazawa

    Cells 8 (8) 2019年8月14日

    DOI: 10.3390/cells8080891  

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    Peptidoglycan recognition proteins (PGLYRPs) are a family of pattern recognition receptors (PRRs) that are able to induce innate immune responses through their binding to peptidoglycan (PGN), lipopolysaccharide, or lipoteichoic acid, or by interacting with other PRR-ligands. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however, their functions in livestock animals have been less explored. In this study, we characterized the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen-presenting cells (APCs) and their modulation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory probiotics referred to as paraimmunobiotics. We demonstrated that PGLYRP-1, -2, -3, and -4 are expressed in PIE cells and APCs from Peyer's patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2. We also showed that PGLYRPs expression in APCs and PIE cells can be modulated by different PRR agonists. By using knockdown PIE cells for TLR2, TLR4, NOD1, and NOD2, or the four PGLYRPs, we demonstrated that PGLYRPs expressions would be required for activation and functioning of TLR2, TLR4, NOD1, and NOD2 in porcine epitheliocytes, but PGLYRPs activation would be independent of those PRR expressions. Importantly, we reported for the first time that PGLYRPs expression can be differentially modulated by paraimmunobiotic bifidobacteria in a strain-dependent manner. These results provide evidence for the use of paraimmunobiotic bifidobacteria as an alternative for the improvement of resistance to intestinal infections or as therapeutic tools for the reduction of the severity of inflammatory damage in diseases in which a role of PGLYRPs-microbe interaction has been demonstrated.

  23. Comparative geochemical evaluation of toxic metals pollution and bacterial communities of industrial effluent tributary and a receiving estuary in Nigeria. 国際誌 査読有り

    Ganiyu O Oyetibo, Keisuke Miyauchi, Yi Huang, Wakako Ikeda-Ohtsubo, Mei-Fang Chien, Matthew O Ilori, Olukayode O Amund, Ginro Endo

    Chemosphere 227 638-646 2019年7月

    DOI: 10.1016/j.chemosphere.2019.04.048  

    ISSN:0045-6535

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    Toxic metals/metalloid contaminations of estuarine sediments due to compromised tributaries arouse significant interest in studying bacterial community that triggers natural attenuation processes. Geo-accumulation index (Igeo), contamination factor (CF), pollution load index (PLI), and Hakanson potential ecological risk index (RI) as a sum of risk factors (Er) were used to quantify toxic metal/metalloid-pollution status of Lagos Lagoon (2W) and 'Iya-Alaro' tributary (4W) sediments in comparison with pristine 'Lekki Conservation Centre' sediment (L1-B). Bacteriology of the ecosystems was based on culture-independent analyses using pyrosequencing. 2W and 4W were extremely contaminated with mercury (Igeo > 7), whereas, cadmium contamination was only observed in 4W. The two ecosystems were polluted with toxic metal based on PLI, where mercury (Er = 2900 and 1900 for 4W and 2W, respectively) posed very high ecological risks. Molecular fingerprinting revealed that Proteobacteria, Firmicutes, and Acidobacteria predominately contributed the 20 most abundant genera in the two ecosystems. The 240 and 310 species present in 2W and 4W, respectively, but absent in L1-B, thrive under the metal concentrations in the polluted hydrosphere. Whereas, the 58,000 species missing in 2W and 4W but found in L1-B would serve as indicators for systems impacted with metal eco-toxicity. Despite toxic metal pollution of the ecosystems understudied, bacterial communities play vital roles in self-recovery processes occurring in the hydrosphere.

  24. Isolation and Immunocharacterization of Lactobacillus salivarius from the Intestine of Wakame-Fed Pigs to Develop Novel "Immunosynbiotics". 国際誌 査読有り

    Yuki Masumizu, Binghui Zhou, A K M Humayun Kober, Md Aminul Islam, Hikaru Iida, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Leonardo Albarracin, Tomonori Nochi, Hisashi Aso, Keiichi Suzuki, Julio Villena, Haruki Kitazawa

    Microorganisms 7 (6) 2019年6月6日

    DOI: 10.3390/microorganisms7060167  

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    Emerging threats of antimicrobial resistance necessitate the exploration of effective alternatives for healthy livestock growth strategies. 'Immunosynbiotics', a combination of immunoregulatory probiotics and prebiotics with synergistic effects when used together in feed, would be one of the most promising candidates. Lactobacilli are normal residents of the gastrointestinal tract of pigs, and many of them are able to exert beneficial immunoregulatory properties. On the other hand, wakame (Undaria pinnafida), an edible seaweed, has the potential to be used as an immunoregulatory prebiotic when added to livestock feed. Therefore, in order to develop a novel immunosynbiotic, we isolated and characterized immunoregulatory lactobacilli with the ability to utilize wakame. Following a month-long in vivo wakame feeding trial in 8-week-old Landrace pigs (n = 6), sections of intestinal mucous membrane were processed for bacteriological culture and followed by identification of pure colonies by 16S rRNA sequence. Each isolate was characterized in vitro in terms of their ability to assimilate to the wakame and to differentially modulate the expression of interleukin-6 (IL-6) and interferon beta (IFN-β) in the porcine intestinal epithelial (PIE) cells triggered by Toll-like receptor (TLR)-4 and TLR-3 activation, respectively. We demonstrated that feeding wakame to pigs significantly increased the lactobacilli population in the small intestine. We established a wakame-component adjusted culture media that allowed the isolation and characterization of a total of 128 Lactobacilli salivarius colonies from the gut of wakame-fed pigs. Interestingly, several L. salivarius isolates showed both high wakame assimilation ability and immunomodulatory capacities. Among the wakame assimilating isolates, L. salivarius FFIG71 showed a significantly higher capacity to upregulate the IL-6 expression, and L. salivarius FFIG131 showed significantly higher capacity to upregulate the IFN-β expression; these could be used as immunobiotic strains in combination with wakame for the development of novel immunologically active feeds for pigs.

  25. Draft Genome Sequence of Weissella viridescens UCO-SMC3, Isolated from the Slime of <i>Helix aspersa</i> Müller Snails. 国際誌 査読有り

    Garcia-Cancino A, Albarracin L, Espinoza-Monje M, Campos-Martin J, Garcia-Castillo V, Nakano Y, Ikeda-Ohtsubo W, Guitierrez-Zamorano C, Morita H, Kitazawa H, Villena J

    Microbiology resource announcements 8 (11) 2019年3月14日

    DOI: 10.1128/MRA.01654-18  

  26. Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation. 国際誌 査読有り

    Manami Igata, Md Aminul Islam, Asuka Tada, Michihiro Takagi, A K M Humayun Kober, Leonardo Albarracin, Hisashi Aso, Wakako Ikeda-Ohtsubo, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Frontiers in immunology 10 (MAY) 1180-1180 2019年

    DOI: 10.3389/fimmu.2019.01180  

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    Adipocytes are the most important cell type in adipose tissue playing key roles in immunometabolism. We previously reported that nine members of the Toll-like receptor (TLR) family are expressed in an originally established porcine intramuscular pre-adipocyte (PPI) cell line. However, the ability of TLR ligands to modulate immunometabolic transcriptome modifications in porcine adipocytes has not been elucidated. Herein, we characterized the global transcriptome modifications in porcine intramuscular mature adipocytes (pMA), differentiated from PPI, following stimulation with Pam3csk4, Poly(I:C) or LPS which are ligands for TLR2, TLR3, and TLR4, respectively. Analysis of microarray data identified 530 (218 up, 312 down), 520 (245 up, 275 down), and 525 (239 up, 286 down) differentially expressed genes (DEGs) in pMA following the stimulation with Pam3csk4, Poly(I:C), and LPS, respectively. Gene ontology classification revealed that DEGs are involved in several biological processes including those belonging to immune response and lipid metabolism pathways. Functionally annotated genes were organized into two groups for downstream analysis: immune response related genes (cytokines, chemokines, complement factors, adhesion molecules, and signal transduction), and genes involved with metabolic and endocrine functions (hormones and receptors, growth factors, and lipid biosynthesis). Differential expression analysis revealed that EGR1, NOTCH1, NOS2, TNFAIP3, TRAF3IP1, INSR, CXCR4, PPARA, MAPK10, and C3 are the top 10 commonly altered genes of TLRs induced transcriptional modification of pMA. However, the protein-protein interaction network of DEGs identified EPOR, C3, STAR, CCL2, and SAA2 as the major hub genes, which were also exhibited higher centrality estimates in the Gene-Transcription factor interaction network. Our results provide new insights of transcriptome modifications associated with TLRs activation in porcine adipocytes and identified key regulatory genes that could be used as biomarkers for the evaluation of treatments having immunomodularoty and/or metabolic functional beneficial effects in porcine adipocytes.

  27. Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells. 国際誌 査読有り

    Paulraj Kanmani, Leonardo Albarracin, Hisakazu Kobayashi, Hikaru Iida, Ryoya Komatsu, A K M Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Seiya Makino, Hiroshi Kano, Tadao Saito, Julio Villena, Haruki Kitazawa

    Molecular immunology 93 253-265 2018年1月

    DOI: 10.1016/j.molimm.2017.07.009  

    ISSN:0161-5890

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    Previous studies demonstrated that the extracellular polysaccharides (EPSs) produced by Lactobacillus delbrueckii OLL1073R-1 (LDR-1) improve antiviral immunity, especially in the systemic and respiratory compartments. However, it was not studied before whether those EPSs are able to beneficially modulate intestinal antiviral immunity. In addition, LDR-1-host interaction has been evaluated mainly with immune cells while its interaction with intestinal epithelial cells (IECs) was not addressed before. In this work, we investigated the capacity of EPSs from LDR-1 to modulate the response of porcine IECs (PIE cells) to the stimulation with the Toll-like receptor (TLR)-3 agonist poly(I:C) and the role of TLR2, TLR4, and TLR negative regulators in the immunoregulatory effect. We showed that innate immune response triggered by TLR3 activation in porcine IECs was differentially modulated by EPS from LDR-1. EPSs treatment induced an increment in the expression of interferon (IFN)-α and IFN-β in PIE cells after the stimulation with poly(I:C) as well as the expression of the antiviral factors MxA and RNase L. Those effects were related to the reduced expression of A20 in EPS-treated PIE cells. EPS from LDR-1 was also able to reduce the expression of IL-6 and proinflammatory chemokines. Although further in vivo studies are needed, our results suggest that these EPSs or a yogurt fermented with LDR-1 have potential to improve intestinal innate antiviral response and protect against intestinal viruses.

  28. Corrigendum: How Can We Define "Optimal Microbiota?": A Comparative Review of Structure and Functions of Microbiota of Animals, Fish, and Plants in Agriculture. 国際誌 査読有り

    Wakako Ikeda-Ohtsubo, Sylvia Brugman, Craig H Warden, Johanna M J Rebel, Gert Folkerts, Corné M J Pieterse

    Frontiers in nutrition 5 113-113 2018年

    DOI: 10.3389/fnut.2018.00113  

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    [This corrects the article DOI: 10.3389/fnut.2018.00090.].

  29. Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells. 国際誌 査読有り

    Paulraj Kanmani, Leonardo Albarracin, Hisakazu Kobayashi, Elvira Maria Hebert, Lucila Saavedra, Ryoya Komatsu, Brian Gatica, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Shintaro Egusa, Takashi Mishima, Alexis Salas-Burgos, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Frontiers in immunology 9 (SEP) 2178-2178 2018年

    出版者・発行元:Frontiers Media {SA}

    DOI: 10.3389/fimmu.2018.02178  

    ISSN:1664-3224

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    In lactic acid bacteria, the synthesis of exopolysaccharides (EPS) has been associated with some favorable technological properties as well as health-promoting benefits. Research works have shown the potential of EPS produced by lactobacilli to differentially modulate immune responses. However, most studies were performed in immune cells and few works have concentrated in the immunomodulatory activities of EPS in non-immune cells such as intestinal epithelial cells. In addition, the cellular and molecular mechanisms involved in the immunoregulatory effects of EPS have not been studied in detail. In this work, we have performed a genomic characterization of Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and evaluated the immunomodulatory and antiviral properties of its acidic (APS) and neutral (NPS) EPS in porcine intestinal epithelial (PIE) cells. Whole genome sequencing allowed the analysis of the general features of L. delbrueckii TUA4408L genome as well as the characterization of its EPS genes. A typical EPS gene cluster was found in the TUA4408L genome consisting in five highly conserved genes epsA-E, and a variable region, which includes the genes for the polymerase wzy, the flippase wzx, and seven glycosyltransferases. In addition, we demonstrated here for the first time that L. delbrueckii TUA4408L and its EPS are able to improve the resistance of PIE cells against rotavirus infection by reducing viral replication and regulating inflammatory response. Moreover, studies in PIE cells demonstrated that the TUA4408L strain and its EPS differentially modulate the antiviral innate immune response triggered by the activation of Toll-like receptor 3 (TLR3). L. delbrueckii TUA4408L and its EPS are capable of increasing the activation of interferon regulatory factor (IRF)-3 and nuclear factor κB (NF-κB) signaling pathways leading to an improved expression of the antiviral factors interferon (IFN)-β, Myxovirus resistance gene A (MxA) and RNaseL.

  30. A Comparative Review on Microbiota Manipulation: Lessons From Fish, Plants, Livestock, and Human Research. 国際誌 査読有り

    Sylvia Brugman, Wakako Ikeda-Ohtsubo, Saskia Braber, Gert Folkerts, Corné M J Pieterse, Peter A H M Bakker

    Frontiers in nutrition 5 80-80 2018年

    出版者・発行元:Frontiers Media {SA}

    DOI: 10.3389/fnut.2018.00080  

    ISSN:2296-861X

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    During recent years the impact of microbial communities on the health of their host (being plants, fish, and terrestrial animals including humans) has received increasing attention. The microbiota provides the host with nutrients, induces host immune development and metabolism, and protects the host against invading pathogens (1-6). Through millions of years of co-evolution bacteria and hosts have developed intimate relationships. Microbial colonization shapes the host immune system that in turn can shape the microbial composition (7-9). However, with the large scale use of antibiotics in agriculture and human medicine over the last decades an increase of diseases associated with so-called dysbiosis has emerged. Dysbiosis refers to either a disturbed microbial composition (outgrowth of possible pathogenic species) or a disturbed interaction between bacteria and the host (10). Instead of using more antibiotics to treat dysbiosis there is a need to develop alternative strategies to combat disturbed microbial control. To this end, we can learn from nature itself. For example, the plant root (or "rhizosphere") microbiome of sugar beet contains several bacterial species that suppress the fungal root pathogen Rhizoctonia solani, an economically important fungal pathogen of this crop (11). Likewise, commensal bacteria present on healthy human skin produce antimicrobial molecules that selectively kill skin pathogen Staphylococcus aureus. Interestingly, patients with atopic dermatitis (inflammation of the skin) lacked antimicrobial peptide secreting commensal skin bacteria (12). In this review, we will give an overview of microbial manipulation in fish, plants, and terrestrial animals including humans to uncover conserved mechanisms and learn how we might restore microbial balance increasing the resilience of the host species.

  31. 硫酸還元細菌の腸管における生態とその研究アプローチ

    大坪 和香子

    無菌生物 47 (1) 30-38 2017年9月

    出版者・発行元:日本無菌生物ノートバイオロジー学会

    ISSN:0910-0903

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    ヒトの腸管内に常在する硫酸還元細菌(sulfate-reducing bacteria、SRB)は、様々な疾患患者の腸内において増加することが報告されている。SRBが産生する硫化水素H2Sの毒性や代謝阻害作用が腸管内の炎症に関与している可能性は大きいが、それだけでは各疾患に特異的なSRBの増加やその影響は説明できない。腸管マイクロバイオーム研究において頻繁に見出されるSRBの生理学的機能を理解するためには、培養研究から多くを学ぶ必要がある。本稿では、ヒト腸管だけではなく地球上のあらゆる嫌気環境において重要な役割をもつSRBのユニークな生態と生存戦略を、主要なSRB研究からの知見と併せて概説する。さらに、腸管内のSRBを研究するための各手法とその注意点について、著者らがこれまでの研究において得た知見を基に解説する。(著者抄録)

  32. Biodegradation of crude oil and phenanthrene by heavy metal resistant Bacillus subtilis isolated from a multi-polluted industrial wastewater creek 査読有り

    Ganiyu Oladunjoye Oyetibo, Mei-Fang Chien, Wakako Ikeda-Ohtsubo, Hitoshi Suzuki, Oluwafemi Sunday Obayori, Sunday Adekunle Adebusoye, Matthew Olusoji Ilori, Olukayode Oladipo Amund, Ginro Endo

    International Biodeterioration & Biodegradation 120 143-151 2017年5月

    出版者・発行元:Elsevier {BV}

    DOI: 10.1016/j.ibiod.2017.02.021  

    ISSN:0964-8305

    eISSN:1879-0208

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    A critical bottleneck associated with bioremediation technology in multi-polluted environments is microbiostasis due to metal toxicity. Autochthonous Bacillus species that would harness a repertory of traits to catabolize hydrocarbons and simultaneously sequester heavy metals (HMs) is invaluable in the environment contaminated with divergent pollutants. Fourteen HM-resistant bacilli from polluted creek were characterized using phenotypic and molecular criteria, and studied for hydrocarbon degradation in chemically defined media amended with Co2+ and Ni2+ (5.0 mmol l(-1) each). Phylogenetic analyses revealed distribution of the bacilli into three clades. Two dissimilar strains of Bacillus subtilis (M16K, and M19F) with 19.1% sequence divergence, exhibited excellent degradation of crude oil (&gt;94.0%) with evidence of early degradation of isoprenoid hydrocarbons and concurrent metal removal 18 d post inoculation. Similarly, phenanthrene degradation (&gt;85.0%), and corresponding metal detoxification occurred in 28 d axenic culture of the strains. Strain M16K and M19F were metabolically active in matrices containing HMs, degraded hydrocarbons and simultaneously removed HMs from the medium. To the best of our knowledge, this is the first report of metal-resistant Bacillus subtilis strains showing simultaneous degradation of hydrocarbons and detoxification of metals, particularly in the Sub-Saharan Africa. The bacilli could be useful as potential biological agents in effective bioremediation campaign for multi-polluted environments. (C) 2017 Elsevier Ltd. All rights reserved.

  33. Draft Genome Sequence of Lactobacillus plantarum MPL16, a Wakame-Utilizing Immunobiotic Strain Isolated from Swine Feces. 国際誌 査読有り

    Julio Villena, Lucila Saavedra, Elvira Maria Hebert, Yoshihito Suda, Yuki Masumizu, Leonardo Albarracin, Patricia Clua, Wakako Ikeda-Ohtsubo, Haruki Kitazawa

    Genome announcements 5 (10) e00006-e00017 2017年3月9日

    出版者・発行元:American Society for Microbiology

    DOI: 10.1128/genomeA.00006-17  

    ISSN:2169-8287

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    The genome of the immunomodulatory Lactobacillus plantarum MPL16, a strain able to ferment wakame (Undaria pinnatifida), is described here. The reads were assembled into contigs with a total size 3,278,495 bp. The genome information will be useful for further specific genetic studies of this strain that evaluate its immunomodulatory and biotechnological properties.

  34. Draft Genome Sequence of the Immunobiotic Strain Lactobacillus jensenii TL2937. 国際誌 査読有り

    Julio Villena, Yuki Masumizu, Hikaru Iida, Wakako Ikeda-Ohtsubo, Leonardo Albarracin, Seiya Makino, Sou Ohkawara, Katsunori Kimura, Lucila Saavedra, Elvira Maria Hebert, Haruki Kitazawa

    Genome announcements 5 (9) 2017年3月2日

    出版者・発行元:American Society for Microbiology

    DOI: 10.1128/genomeA.00005-17  

    ISSN:2169-8287

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    The genome of the immunomodulatory strain Lactobacillus jensenii TL2937 is described here. The draft genome has a total length of 1,678,416 bp, a G+C content of 34.3%, and 1,470 predicted protein-coding sequences. The genome information will be useful for gaining insight into the immunomodulatory properties of the TL2937 strain in the porcine host.

  35. Draft Genome Sequence of Lactobacillus plantarum TL2766, a Strain with the Ability To Ferment Wakame. 国際誌 査読有り

    Julio Villena, Lucila Saavedra, Elvira Maria Hebert, Yuki Masumizu, Nana Sato, A K M Humayun Kober, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Seiya Makino, Katsunori Kimura, Sou Ohkawara, Haruki Kitazawa

    Genome announcements 4 (6) 2016年11月23日

    出版者・発行元:American Society for Microbiology

    DOI: 10.1128/genomeA.01328-16  

    ISSN:2169-8287

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    The genome sequence of Lactobacillus plantarum TL2766, a strain with the ability to ferment wakame (Undaria pinnatifida), is described here. The reads were assembled into contigs, with a total size of 3,310,195 bp. The genome information will be useful for further specific genetic studies of this strain and for its biotechnological applications.

  36. Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937 査読有り

    Kobayashi, H., Albarracin, L., Sato, N., Kanmani, P., Kober, A.K.M.H., Ikeda-Ohtsubo, W., Suda, Y., Nochi, T., Aso, H., Makino, S., Kano, H., Ohkawara, S., Saito, T., Villena, J., Kitazawa, H.

    Beneficial Microbes 7 (5) 769-782 2016年

    出版者・発行元:WAGENINGEN ACADEMIC PUBLISHERS

    DOI: 10.3920/BM2016.0095  

    ISSN:1876-2883

    eISSN:1876-2891

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    In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains. We performed microarray analysis to investigate the transcriptomic response of PIE cells to the challenge with heat-stable enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs) and, the changes induced by L. jensenii TL2937 in that response. The approach allowed us to obtain a global overview of the immune genes involved in the response of PIE cells to heat-stable ETEC PAMPs. We observed that L. jensenii TL2937 differently modulated gene expression in ETEC PAMPs-challenged PIE cells. Microarray and RT-PCR analysis indicated that the most remarkable changes in PIE cells transcriptomic profile after heat-stable ETEC PAMPs challenge were observed in chemokines, adhesion molecules, complement and coagulation cascades factors. In addition, an anti-inflammatory effect triggered by TL2937 strain in PIE cells was clearly demonstrated. The decrease in the expression of chemokines (CCL8, CXCL5, CXCL9, CXCL10, and CXCL11), complement (C1R, C1S, C3, and CFB), and coagulation factors (F3) by L. jensenii TL2937 supports our previous reports on the immunoregulatory effect of this strain. These results provided clues for the better understanding of the mechanism underlying host-immunobiotic interaction in the porcine host. The comprehensive transcriptomic profiles of PIE cells provided by our analyses successfully identified a group of genes, which could be used as prospective biomarkers for the screening and evaluation of new anti-inflammatory immunobiotics for the prevention of inflammatory intestinal disorders in pigs.

  37. Mercury bioremoval by Yarrowia strains isolated from sediments of mercury-polluted estuarine water. 国際誌 査読有り

    Ganiyu Oladunjoye Oyetibo, Shakirat Titilayo Ishola, Wakako Ikeda-Ohtsubo, Keisuke Miyauchi, Matthew Olusoji Ilori, Ginro Endo

    Applied microbiology and biotechnology 99 (8) 3651-7 2015年4月

    出版者・発行元:Springer Nature

    DOI: 10.1007/s00253-014-6279-1  

    ISSN:0175-7598

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    Difference in mercuric ion removal by resting and growing cells of two mercury-resistant yeast strains, identified as Yarrowia spp. (strains Idd1 and Idd2), were studied. Resting cells of strain Idd2 exhibited high maximum Hg(2+) removal capacity (59 mg mercury per g dry cell weight [gdw(-1)]) by adsorption than those of resting cells of strain Idd1 (32 mg gdw(-1)). The resting cells of strain Idd2 exhibited a higher Hg(2+) desorption capacity using CaCl2 (68 %) and EDTA (48 %) than strain Idd1, depicting weaker binding of Hg(2+) onto strain Idd2 unlike strain Idd1. The actively growing yeast cells showed opposite Hg removal characteristics to those of the resting cells. Strain Idd1 adsorbed less Hg(2+) from culture medium supplemented with Hg(2+) than strain Idd2. However, the growing strain Idd1 reduced and vaporized 27 % of supplemented Hg(2+) as metallic mercury (Hg(0)), while the growing strains Idd2 vaporized 15 % of the supplemented Hg(2+). These two yeast strains are potential biotechnological tools for the eventual bioremediation of polluted aquatic systems.

  38. 畜産廃水処理システム由来の固形廃棄物の堆肥化における環境負荷低減化

    大坪和香子, 進藤絵里香, 山田剛史, 上田英代, 上田裕一, 渡辺昭, 宮内啓介, 遠藤銀朗

    環境バイオテクノロジー学会誌 15 (1) 17-22 2015年

    出版者・発行元:環境バイオテクノロジー学会

  39. Structural and functional analysis of a novel haloalkane dehalogenase with two halide-binding sites. 国際誌 査読有り

    Radka Chaloupkova, Tatyana Prudnikova, Pavlina Rezacova, Zbynek Prokop, Tana Koudelakova, Lukas Daniel, Jan Brezovsky, Wakako Ikeda-Ohtsubo, Yukari Sato, Michal Kuty, Yuji Nagata, Ivana Kuta Smatanova, Jiri Damborsky

    Acta crystallographica. Section D, Biological crystallography 70 (Pt 7) 1884-97 2014年7月

    DOI: 10.1107/S1399004714009018  

    ISSN:0907-4449

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    The crystal structure of the novel haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94 revealed the presence of two chloride ions buried in the protein interior. The first halide-binding site is involved in substrate binding and is present in all structurally characterized haloalkane dehalogenases. The second halide-binding site is unique to DbeA. To elucidate the role of the second halide-binding site in enzyme functionality, a two-point mutant lacking this site was constructed and characterized. These substitutions resulted in a shift in the substrate-specificity class and were accompanied by a decrease in enzyme activity, stability and the elimination of substrate inhibition. The changes in enzyme catalytic activity were attributed to deceleration of the rate-limiting hydrolytic step mediated by the lower basicity of the catalytic histidine.

  40. Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor. 査読有り

    Wakako Ikeda-Ohtsubo, Morio Miyahara, Takeshi Yamada, Akira Watanabe, Shinya Fushinobu, Takayoshi Wakagi, Hirofumi Shoun, Keisuke Miyauchi, Ginro Endo

    Journal of bioscience and bioengineering 116 (6) 722-4 2013年12月

    出版者・発行元:Elsevier {BV}

    DOI: 10.1016/j.jbiosc.2013.05.024  

    ISSN:1389-1723

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    Bioaugmentation of bioreactor systems with pre-cultured bacteria has proven difficult because inoculated bacteria are easily eliminated by predatory eukaryotic-microorganisms. Here, we demonstrated an intermediate thermal treatment was effective for protecting introduced denitrifying bacteria from eukaryotic predators and consequently allowed the inoculated bacteria to survive longer in a denitrification reactor.

  41. Community structure and population dynamics of ammonia oxidizers in composting processes of ammonia-rich livestock waste. 国際誌 査読有り

    Takeshi Yamada, Shinya Araki, Wakako Ikeda-Ohtsubo, Keiko Okamura, Akira Hiraishi, Hideyo Ueda, Yasuichi Ueda, Keisuke Miyauchi, Ginro Endo

    Systematic and applied microbiology 36 (5) 359-67 2013年7月

    出版者・発行元:Elsevier {BV}

    DOI: 10.1016/j.syapm.2013.02.001  

    ISSN:0723-2020

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    This study investigated the relationship between the population dynamics of ammonia-oxidizing bacteria (AOB) and archaea (AOA), and changes in the concentrations of nitrogenous compounds during ammonia-rich livestock waste-composting processes. The data showed that ammonia in beef and dairy cow livestock waste-composting piles was slowly oxidized to nitrite and nitrate after approximately 21-35 days under thermophilic or moderately thermophilic and mesophilic conditions. Real-time quantitative PCR (qPCR) assays showed a relative abundance of betaproteobacterial AOB during ammonia oxidation but did not detect AOA in any composting stage. Furthermore, real-time qPCR and terminal-restriction fragment length polymorphism (T-RFLP) analyses for the AOB in two composting processes (beef and dairy cow livestock waste) out of the three studied found that thermophilic or moderately thermophilic uncultured betaproteobacterial AOB from the "compost AOB cluster" contributed to ammonia oxidation during hot composting stages. Non-metric multidimensional scaling analyses of the data from T-RFLP showed that only a few analogous species predominated during composting of beef, dairy cow and pig livestock wastes, and thus, the AOB community structures in the three composting piles operating under different conditions were similar. AOB-targeted clone library analyses revealed that uncultured members of the "compost AOB cluster", which could be clearly distinguished from the authentic species of the genus Nitrosomonas, were the major constituents of the AOB populations. These results suggested that a limited and unique species of AOB played a role in ammonia oxidation during the composting of ammonia-rich livestock waste.

  42. Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2. 査読有り

    Wakako Ikeda-Ohtsubo, Morio Miyahara, Sang-Wan Kim, Takeshi Yamada, Masaki Matsuoka, Akira Watanabe, Shinya Fushinobu, Takayoshi Wakagi, Hirofumi Shoun, Keisuke Miyauchi, Ginro Endo

    Journal of bioscience and bioengineering 115 (1) 37-42 2013年1月

    出版者・発行元:Elsevier {BV}

    DOI: 10.1016/j.jbiosc.2012.08.015  

    ISSN:1389-1723

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    In bioaugmentation technology, survival of inoculant in the treatment system is prerequisite but remains to be a crucial hurdle. In this study, we bioaugmented the denitrification tank of a piggery wastewater treatment system with the denitrifying bacterium Pseudomonas stutzeri strain TR2 in two pilot-scale experiments, with the aim of reducing nitrous oxide (N(2)O), a gas of environmental concern. In the laboratory, strain TR2 grew well and survived with high concentrations of nitrite (5-10 mM) at a wide range of temperatures (28-40°C). In the first augmentation of the pilot-scale experiment, strain TR2 inoculated into the denitrification tank with conditions (30°C, ~0.1 mM nitrite) survived only 2-5 days. In contrast, in the second augmentation with conditions determined to be favorable for the growth of the bacterium in the laboratory (40-45°C, 2-5 mM nitrite), strain TR2 survived longer than 32 days. During the time when the presence of strain TR2 was confirmed by quantitative real-time PCR, N(2)O emission was maintained at a low level even under nitrite-accumulating conditions in the denitrification and nitrification tanks, which provided indirect evidence that strain TR2 can reduce N(2)O in the pilot-scale system. Our results documented the effective application of growth conditions favorable for strain TR2 determined in the laboratory to maintain growth and performance of this strain in the pilot-scale reactor system and the decrease of N(2)O emission as the consequence.

  43. 'Candidatus Ancillula trichonymphae', a novel lineage of endosymbiotic Actinobacteria in termite gut flagellates of the genus Trichonympha. 国際誌 査読有り

    Jürgen F H Strassert, Tim Köhler, Tobias H G Wienemann, Wakako Ikeda-Ohtsubo, Nicolas Faivre, Sibylle Franckenberg, Rudy Plarre, Renate Radek, Andreas Brune

    Environmental microbiology 14 (12) 3259-70 2012年12月

    出版者・発行元:Wiley

    DOI: 10.1111/1462-2920.12012  

    ISSN:1462-2912

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    Termite gut flagellates are colonized by host-specific lineages of ectosymbiotic and endosymbiotic bacteria. Previous studies have shown that flagellates of the genus Trichonympha may harbour more than one type of symbiont. Using a comprehensive approach that combined cloning of SSU rRNA genes with fluorescence in situ hybridization and electron microscopy, we investigated the phylogeny and subcellular locations of the symbionts in a variety of Trichonympha species from different termites. The flagellates in Trichonympha Cluster I were the only species associated with 'Endomicrobia', which were located in the posterior part of the cell, confirming previous results. Trichonympha species of Cluster II from the termite genus Incisitermes (family Kalotermitidae) lacked 'Endomicrobia' and were associated with endosymbiotic Actinobacteria, which is highly unusual. The endosymbionts, for which we suggest the name 'Candidatus Ancillula trichonymphae', represent a novel, deep-branching lineage in the Micrococcineae that consists exclusively of clones from termite guts. They preferentially colonized the anterior part of the flagellate host and were highly abundant in all species of Trichonympha Cluster II except Trichonympha globulosa. Here, they were outnumbered by a Desulfovibrio species associated with the cytoplasmic lamellae at the anterior cell pole. Such symbionts are present in both Trichonympha clusters, but not in all species. Unlike the intracellular location reported for the Desulfovibrio symbionts of Trichonympha agilis (Cluster I), the Desulfovibrio symbionts of T. globulosa (Cluster II) were situated in deep invaginations of the plasma membrane that were clearly connected to the exterior of the host cell.

  44. Complete genome sequence of Bradyrhizobium sp. S23321: insights into symbiosis evolution in soil oligotrophs. 査読有り

    Takashi Okubo, Takahiro Tsukui, Hiroko Maita, Shinobu Okamoto, Kenshiro Oshima, Takatomo Fujisawa, Akihiro Saito, Hiroyuki Futamata, Reiko Hattori, Yumi Shimomura, Shin Haruta, Sho Morimoto, Yong Wang, Yoriko Sakai, Masahira Hattori, Shin-Ichi Aizawa, Kenji V P Nagashima, Sachiko Masuda, Tsutomu Hattori, Akifumi Yamashita, Zhihua Bao, Masahito Hayatsu, Hiromi Kajiya-Kanegae, Ikuo Yoshinaga, Kazunori Sakamoto, Koki Toyota, Mitsuteru Nakao, Mitsuyo Kohara, Mizue Anda, Rieko Niwa, Park Jung-Hwan, Reiko Sameshima-Saito, Shin-Ichi Tokuda, Sumiko Yamamoto, Syuji Yamamoto, Tadashi Yokoyama, Tomoko Akutsu, Yasukazu Nakamura, Yuka Nakahira-Yanaka, Yuko Takada Hoshino, Hideki Hirakawa, Hisayuki Mitsui, Kimihiro Terasawa, Manabu Itakura, Shusei Sato, Wakako Ikeda-Ohtsubo, Natsuko Sakakura, Eli Kaminuma, Kiwamu Minamisawa

    Microbes and environments 27 (3) 306-15 2012年

    DOI: 10.1264/jsme2.ME11321  

    ISSN:1342-6311

    eISSN:1347-4405

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    Bradyrhizobium sp. S23321 is an oligotrophic bacterium isolated from paddy field soil. Although S23321 is phylogenetically close to Bradyrhizobium japonicum USDA110, a legume symbiont, it is unable to induce root nodules in siratro, a legume often used for testing Nod factor-dependent nodulation. The genome of S23321 is a single circular chromosome, 7,231,841 bp in length, with an average GC content of 64.3%. The genome contains 6,898 potential protein-encoding genes, one set of rRNA genes, and 45 tRNA genes. Comparison of the genome structure between S23321 and USDA110 showed strong colinearity; however, the symbiosis islands present in USDA110 were absent in S23321, whose genome lacked a chaperonin gene cluster (groELS3) for symbiosis regulation found in USDA110. A comparison of sequences around the tRNA-Val gene strongly suggested that S23321 contains an ancestral-type genome that precedes the acquisition of a symbiosis island by horizontal gene transfer. Although S23321 contains a nif (nitrogen fixation) gene cluster, the organization, homology, and phylogeny of the genes in this cluster were more similar to those of photosynthetic bradyrhizobia ORS278 and BTAi1 than to those on the symbiosis island of USDA110. In addition, we found genes encoding a complete photosynthetic system, many ABC transporters for amino acids and oligopeptides, two types (polar and lateral) of flagella, multiple respiratory chains, and a system for lignin monomer catabolism in the S23321 genome. These features suggest that S23321 is able to adapt to a wide range of environments, probably including low-nutrient conditions, with multiple survival strategies in soil and rhizosphere.

  45. Survival of the aerobic denitrifier Pseudomonas stutzeri strain TR2 during co-culture with activated sludge under denitrifying conditions. 国際誌 査読有り

    Morio Miyahara, Sang-Wan Kim, Shengmin Zhou, Shinya Fushinobu, Takeshi Yamada, Wakako Ikeda-Ohtsubo, Akira Watanabe, Keisuke Miyauchi, Ginro Endo, Takayoshi Wakagi, Hirofumi Shoun

    Bioscience, biotechnology, and biochemistry 76 (3) 495-500 2012年

    出版者・発行元:Informa {UK} Limited

    DOI: 10.1271/bbb.110785  

    ISSN:0916-8451

    eISSN:1347-6947

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    The aerobic denitrifier Pseudomonas stutzeri TR2 (strain TR2) has the potential to reduce nitrous oxide emissions during the wastewater treatment process. In this application, it is important to find the best competitive survival conditions for strain TR2 in complex ecosystems. To that end, we examined co-cultures of strain TR2 with activated sludge via five passage cultures in a medium derived from treated piggery wastewater that contained a high concentration of ammonium. The results are as follows: (i) The medium supported the proliferation of strain TR2 (P. stutzeri strains) under denitrifying conditions. (ii) Nitrite was a better denitrification substrate than nitrate for TR2 survival. (iii) Strain TR2 also demonstrated strong survival even under aerobic conditions. This suggests that strain TR2 is effectively augmented to the wastewater treatment process, aiding in ammonium-nitrogen removal and reducing nitrous oxide production with a partial nitrification technique in which nitrite accumulates.

  46. コンポスト製造過程において発生する亜酸化窒素の削減に関与する脱窒細菌遺伝子の多様性と消長 査読有り

    進藤絵里香, 大坪和香子, 上田裕一, 上田英代, 宮内啓介, 遠藤銀朗

    土木学会論文集G(環境) 2011年

  47. Putatively free-living 'Endomicrobia'- ancestors of the intracellular symbionts of termite gut flagellates? 国際誌 査読有り

    Wakako Ikeda-Ohtsubo, Nicolas Faivre, Andreas Brune

    Environmental microbiology reports 2 (4) 554-9 2010年8月

    出版者・発行元:Wiley

    DOI: 10.1111/j.1758-2229.2009.00124.x  

    ISSN:1758-2229

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    Endomicrobia represent a candidate class in the Elusimicrobia phylum (formerly Termite Group 1) and were originally described as obligate intracellular symbionts of gut flagellates in lower termites. However, 16S rRNA gene sequences of Endomicrobia have been detected also in the gut of insects that do not possess such flagellates, e.g. higher termites and cockroaches. When we eliminated the large gut flagellates of Reticulitermes santonensis by feeding a starch diet, we discovered novel lineages of Endomicrobia that were hitherto undetected in normally faunated specimens. The new phylotypes are clearly separated from the endosymbionts of gut flagellates and fall into the radiation of those from flagellate-free insects. Comprehensive phylogenetic analysis documented that Endomicrobia comprise an apical cluster of endosymbionts that is not necessarily monophyletic and several apparently basal lineages that include bacteria present in the gut of defaunated lower termites, the naturally flagellate-free guts of higher termites and scarab beetle larvae, and in the cow rumen. We propose that these lineages represent hitherto undetected free-living Endomicrobia that share a common ancestor with the intracellular symbionts.

  48. Strict cospeciation of devescovinid flagellates and Bacteroidales ectosymbionts in the gut of dry-wood termites (Kalotermitidae). 国際誌 査読有り

    Mahesh S Desai, Jürgen F H Strassert, Katja Meuser, Horst Hertel, Wakako Ikeda-Ohtsubo, Renate Radek, Andreas Brune

    Environmental microbiology 12 (8) 2120-32 2010年8月

    出版者・発行元:Wiley

    DOI: 10.1111/j.1462-2920.2009.02080.x  

    ISSN:1462-2912

    詳細を見る 詳細を閉じる

    The surface of many termite gut flagellates is colonized with a dense layer of bacteria, yet little is known about the evolutionary relationships of such ectosymbionts and their hosts. Here we investigated the molecular phylogenies of devescovinid flagellates (Devescovina spp.) and their symbionts from a wide range of dry-wood termites (Kalotermitidae). From species-pure flagellate suspensions isolated with micropipettes, we obtained SSU rRNA gene sequences of symbionts and host. Phylogenetic analysis showed that the Devescovina spp. present in many species of Kalotermitidae form a monophyletic group, which includes also the unique devescovinid flagellate Caduceia versatilis. All members of this group were consistently associated with a distinct lineage of Bacteroidales, whose location on the cell surface was confirmed by fluorescence in situ hybridization. The well-supported congruence of the phylogenies of devescovinids and their ectosymbionts documents a strict cospeciation. In contrast, the endosymbionts of the same flagellates ('Endomicrobia') were clearly polyphyletic and must have been acquired independently by horizontal transfer from other flagellate lineages. Also the Bacteroidales ectosymbionts of Oxymonas flagellates present in several Kalotermitidae belonged to several distantly related lines of descent, underscoring the general perception that the evolutionary history of flagellate-bacteria symbioses in the termite gut is complex.

  49. ゲノム解析ソフトウエアGenomeMatcher

    大坪嘉行, 大坪和香子, 永田裕二, 津田雅孝

    化学と生物 48 (5) 313-319 2010年5月1日

    出版者・発行元:公益社団法人 日本農芸化学会

    DOI: 10.1271/kagakutoseibutsu.48.313  

    ISSN:0453-073X

    詳細を見る 詳細を閉じる

    ゲノム情報のような多量の情報を効率良く取り扱うには,個々の研究者が情報処理技術を有していることが望ましい.しかし,多くのいわゆるウエットな研究者は,プログラミングやコマンドラインツールの取り扱いを苦手としており,グラフィカルなユーザーインターフェースを備えたソフトウエアが必要である.ここでは,ゲノム解析関連だけでなく情報処理に有用な機能を備えたソフトウエア,GenomeMatcherを紹介する.

  50. Cospeciation of termite gut flagellates and their bacterial endosymbionts: Trichonympha species and 'Candidatus Endomicrobium trichonymphae'. 国際誌 査読有り

    Wakako Ikeda-Ohtsubo, Andreas Brune

    Molecular ecology 18 (2) 332-42 2009年1月

    出版者・発行元:Wiley

    DOI: 10.1111/j.1365-294X.2008.04029.x  

    ISSN:0962-1083

    eISSN:1365-294X

    詳細を見る 詳細を閉じる

    Symbiotic flagellates play a major role in the digestion of lignocellulose in the hindgut of lower termites. Many termite gut flagellates harbour a distinct lineage of bacterial endosymbionts, so-called Endomicrobia, which belong to the candidate phylum Termite Group 1. Using an rRNA-based approach, we investigated the phylogeny of Trichonympha, the predominant flagellates in a wide range of termite species, and of their Endomicrobia symbionts. We found that Trichonympha species constitute three well-supported clusters in the Parabasalia tree. Endomicrobia were detected only in the apical lineage (Cluster I), which comprises flagellates present in the termite families Termopsidae and Rhinotermitidae, but apparently absent in the basal lineages (Clusters II and III) consisting of flagellates from other termite families and from the wood-feeding cockroach, Cryptocercus punctulatus. The endosymbionts of Cluster I form a monophyletic group distinct from many other lineages of Endomicrobia and seem to have cospeciated with their flagellate host. The distribution pattern of the symbiotic pairs among different termite species indicates that cospeciation of flagellates and endosymbionts is not simply the result of a spatial separation of the flagellate lineages in different termite species, but that Endomicrobia are inherited among Trichonympha species by vertical transmission. We suggest extending the previously proposed candidatus name 'Endomicrobium trichonymphae' to all Endomicrobia symbionts of Trichonympha species, and estimate that the acquisition by an ancestor of Trichonympha Cluster I must have occurred about 40-70 million years ago, long after the flagellates entered the termites.

  51. Genomic analysis of "Elusimicrobium minutum" the first cultivated representative of the phylum "Elusimicrobia" (formerly termite group 1) 査読有り

    Herlemann, D.P.R., Geissinger, O., Ikeda-Ohtsubo, W., Kunin, V., Sun, H., Lapidus, A., Hugenholtz, P., Brune, A.

    Applied and Environmental Microbiology 75 (9) 2841-2849 2009年

    出版者・発行元:AMER SOC MICROBIOLOGY

    DOI: 10.1128/AEM.02698-08  

    ISSN:0099-2240

    詳細を見る 詳細を閉じる

    Organisms of the candidate phylum termite group 1 (TG1) are regularly encountered in termite hindguts but are present also in many other habitats. Here, we report the complete genome sequence (1.64 Mbp) of "Elusimicrobium minutum" strain Pei191(T), the first cultured representative of the TG1 phylum. We reconstructed the metabolism of this strictly anaerobic bacterium isolated from a beetle larva gut, and we discuss the findings in light of physiological data. E. minutum has all genes required for uptake and fermentation of sugars via the Embden-Meyerhof pathway, including several hydrogenases, and an unusual peptide degradation pathway comprising transamination reactions and leading to the formation of alanine, which is excreted in substantial amounts. The presence of genes encoding lipopolysaccharide biosynthesis and the presence of a pathway for peptidoglycan formation are consistent with ultrastructural evidence of a gram-negative cell envelope. Even though electron micrographs showed no cell appendages, the genome encodes many genes putatively involved in pilus assembly. We assigned some to a type II secretion system, but the function of 60 pilE-like genes remains unknown. Numerous genes with hypothetical functions, e. g., polyketide synthesis, nonribosomal peptide synthesis, antibiotic transport, and oxygen stress protection, indicate the presence of hitherto undiscovered physiological traits. Comparative analysis of 22 concatenated single-copy marker genes corroborated the status of "Elusimicrobia" (formerly TG1) as a separate phylum in the bacterial domain, which was so far based only on 16S rRNA sequence analysis.

  52. GenomeMatcher: a graphical user interface for DNA sequence comparison. 国際誌 査読有り

    Yoshiyuki Ohtsubo, Wakako Ikeda-Ohtsubo, Yuji Nagata, Masataka Tsuda

    BMC bioinformatics 9 (1) 376-376 2008年9月16日

    出版者・発行元:Springer Nature

    DOI: 10.1186/1471-2105-9-376  

    ISSN:1471-2105

    詳細を見る 詳細を閉じる

    BACKGROUND: The number of available genome sequences is increasing, and easy-to-use software that enables efficient comparative analysis is needed. RESULTS: We developed GenomeMatcher, a stand-alone software package for Mac OS X. GenomeMatcher executes BLAST and MUMmer, and the detected similarities are displayed in two-dimensional and parallel views with similarity values indicated by color. Selection and re-computation of any subregions is easily performed and allows flexible and in-depth analysis. Furthermore, symbols for annotation data are displayed along the views, and the user can relate the genomic differences with annotation data. While bl2seq allows sub-Giga base comparison, three alignment programs, bl2seq, MAFFT and ClustalW, together with a dotmatch program allow comparative analysis of single-nucleotide level resolution. GenomeMatcher images can be saved as PDF and TIFF files for presentation. As examples of graphical ability of GenomeMatcher to show similarity in colors, we show two cases in Burkholderia and Vivrio strains that the nucleotide sequence of the second largest chromosome changes more rapidly than the largest chromosome. CONCLUSION: GenomeMatcher is efficient and easy-to-use stand-alone software for in-depth comparative analysis of two sequences. GenomeMatcher is useful for detecting similarities in DNA sequences ranging in size from a few to sub-Giga bases.

  53. Phylogenetic diversity of 'Endomicrobia' and their specific affiliation with termite gut flagellates. 国際誌 査読有り

    Wakako Ikeda-Ohtsubo, Mahesh Desai, Ulrich Stingl, Andreas Brune

    Microbiology (Reading, England) 153 (Pt 10) 3458-3465 2007年10月

    出版者・発行元:Microbiology Society

    DOI: 10.1099/mic.0.2007/009217-0  

    ISSN:1350-0872

    詳細を見る 詳細を閉じる

    'Endomicrobia', a distinct and diverse group of uncultivated bacteria in the candidate phylum Termite Group I (TG-1), have been found exclusively in the gut of lower termites and wood-feeding cockroaches. In a previous study, we had demonstrated that the 'Endomicrobia' clones retrieved from Reticulitermes santonensis represent intracellular symbionts of the two major gut flagellates of this termite. Here, we document that 'Endomicrobia' are present also in many other gut flagellates of lower termites. Phylogeny and host specificity of 'Endomicrobia' were investigated by cloning and sequencing of the small subunit rRNA genes of the flagellate and the symbionts, which originated from suspensions of individual flagellates isolated by micropipette. Each flagellate harboured a distinct phylogenetic lineage of 'Endomicrobia'. The results of fluorescent in situ hybridization with 'Endomicrobia'-specific oligonucleotide probes corroborated that 'Endomicrobia' are intracellular symbionts specifically affiliated with their flagellate hosts. Interestingly, the 'Endomicrobia' sequences obtained from flagellates belonging to the genus Trichonympha formed a monophyletic group, suggesting co-speciation between symbiont and host.

︎全件表示 ︎最初の5件までを表示

MISC 54

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    出版者・発行元:(株)ニュー・サイエンス社

    ISSN:1347-4340

    詳細を見る 詳細を閉じる

    炎症性腸疾患(IBD)の発症には宿主因子や環境因子の他に、腸内フローラが大きく関与していると言われている。プロバイオティクス(ビフィズス菌などの有用菌)やプレバイオティクス(有用菌の栄養となる多糖類)による腸内フローラの修復・正常化が腸管内の炎症の抑制や治癒に効果的であることは、多くの動物モデル研究により実証されているが、IBDの治療法としては未だ発展途上である。筆者らは、IBD患者の腸管に不足する酪酸生成細菌と、ビフィズス菌との間で行われる代謝産物のやり取り(クロスフィーディング)に着目し、それを応用した「次世代プロバイオティクス」の提案・開発に向けた研究を行っている。本稿では、その研究背景について、最新の知見を交えながら紹介する。(著者抄録)

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  25. ブタ腸管由来ワカメ資化性乳酸菌のイムノバイオティック利用性

    舛水由季, 飯田ひかる, 須田義人, 大坪和香子, 麻生久, 齋藤忠夫, 鈴木啓一, 北澤春樹

    日本畜産学会大会講演要旨 122nd 198 2017年3月28日

    ISSN:1342-4688

  26. 炎症性腸疾患を悪化させる硫酸基還元細菌に対するビフィズス菌の付着阻害作用の研究

    日下練介, 大坪和香子, 北澤春樹, 齋藤忠夫

    日本畜産学会大会講演要旨 122nd 199 2017年3月28日

    ISSN:1342-4688

  27. 高シュウ酸植物を摂食するハムシ腸内フローラの特性解析

    大坪和香子, 宮城敦子, LEE Michael, Kwan Fu, 森宙史, 黒川顕, 小島紀幸

    日本農芸化学会大会講演要旨集(Web) 2017 ROMBUNNO.2C19a01 (WEB ONLY) 2017年3月5日

    ISSN:2186-7976

  28. ブタ腸管におけるペプチドグリカン認識タンパク質の発現とその調節機構

    飯田 ひかる, 遠野 雅徳, 須田 義人, 大坪 和香子, 齋藤 忠夫, Julio Villena, 北澤 春樹

    日本畜産学会大会講演要旨集 122回 104-104 2017年3月

    出版者・発行元:(公社)日本畜産学会

    ISSN:1342-4688

  29. ブタ腸管由来ワカメ資化性乳酸菌のイムノバイオティック利用性

    舛水 由季, 飯田 ひかる, 須田 義人, 大坪 和香子, 麻生 久, 齋藤 忠夫, 鈴木 啓一, 北澤 春樹

    日本畜産学会大会講演要旨集 122回 198-198 2017年3月

    出版者・発行元:(公社)日本畜産学会

    ISSN:1342-4688

  30. 炎症性腸疾患を悪化させる硫酸基還元細菌に対するビフィズス菌の付着阻害作用の研究

    日下 練介, 大坪 和香子, 北澤 春樹, 齋藤 忠夫

    日本畜産学会大会講演要旨集 122回 199-199 2017年3月

    出版者・発行元:(公社)日本畜産学会

    ISSN:1342-4688

  31. Biodegradation of crude oil and phenanthrene by heavy metal resistant Bacillus subtilis isolated from a multi-polluted industrial wastewater creek

    Oyetibo, G.O., Chien, M.-F., Ikeda-Ohtsubo, W., Suzuki, H., Obayori, O.S., Adebusoye, S.A., Ilori, M.O., Amund, O.O., Endo, G.

    International Biodeterioration and Biodegradation 120 143-151 2017年

    出版者・発行元:ELSEVIER SCI LTD

    DOI: 10.1016/j.ibiod.2017.02.021  

    ISSN:0964-8305

    eISSN:1879-0208

    詳細を見る 詳細を閉じる

    A critical bottleneck associated with bioremediation technology in multi-polluted environments is microbiostasis due to metal toxicity. Autochthonous Bacillus species that would harness a repertory of traits to catabolize hydrocarbons and simultaneously sequester heavy metals (HMs) is invaluable in the environment contaminated with divergent pollutants. Fourteen HM-resistant bacilli from polluted creek were characterized using phenotypic and molecular criteria, and studied for hydrocarbon degradation in chemically defined media amended with Co2+ and Ni2+ (5.0 mmol l(-1) each). Phylogenetic analyses revealed distribution of the bacilli into three clades. Two dissimilar strains of Bacillus subtilis (M16K, and M19F) with 19.1% sequence divergence, exhibited excellent degradation of crude oil (&gt;94.0%) with evidence of early degradation of isoprenoid hydrocarbons and concurrent metal removal 18 d post inoculation. Similarly, phenanthrene degradation (&gt;85.0%), and corresponding metal detoxification occurred in 28 d axenic culture of the strains. Strain M16K and M19F were metabolically active in matrices containing HMs, degraded hydrocarbons and simultaneously removed HMs from the medium. To the best of our knowledge, this is the first report of metal-resistant Bacillus subtilis strains showing simultaneous degradation of hydrocarbons and detoxification of metals, particularly in the Sub-Saharan Africa. The bacilli could be useful as potential biological agents in effective bioremediation campaign for multi-polluted environments. (C) 2017 Elsevier Ltd. All rights reserved.

  32. シロアリ腸内のスピロヘータと仲間たち~木質多糖の嫌気発酵を中心とする進化型微生物生態系~

    大坪和香子

    レプトスピラ・シンポジウム 54th 7 2017年

  33. シロアリ腸内フローラ研究から見た腸内共生系の進化と機能

    大坪和香子

    無菌生物 46 (1) 34(JA),35(EN) 2016年9月1日

    ISSN:0910-0903

  34. ブタにおけるペプチドグリカン認識タンパク質ファミリーの発現とその調節

    飯田ひかる, 遠野雅徳, 須田義人, 麻生久, 大坪和香子, 齋藤忠夫, 北澤春樹

    日本畜産学会大会講演要旨 120th 104 2015年9月11日

    ISSN:1342-4688

  35. 畜産廃水処理システム由来の固形廃棄物の堆肥化における環境負荷低減化

    大坪和香子, 上田英代, 上田裕一, 渡辺昭, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web) 2015 4SY22-3 (WEB ONLY) 2015年3月5日

    ISSN:2186-7976

  36. エゾノギシギシを摂食するGastrophysa属ハムシ腸内フローラの地域的差異

    大坪和香子, 宮城敦子, 小島紀幸, 川合真紀, 内宮博文, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web) 2015 4A34A15 (WEB ONLY) 2015年3月5日

    ISSN:2186-7976

  37. ミクロな動物園 : 腸内微生物の豊かな個性

    大坪 和香子

    東北学院大学工学総合研究所紀要 Bulletin of Research Institute for Engineering and Technology, Tohoku Gakuin University (4) 329-373 2015年3月

    出版者・発行元:東北学院大学工学総合研究所

  38. VOL.16 大坪和香子(スポットライト : イケメン&イケジョ)

    大坪 和香子

    日本微生物生態学会誌 30 (1) 29-30 2015年

    出版者・発行元:日本微生物生態学会

    DOI: 10.20709/jsmeja.30.1_29  

  39. 6th KJT symposium報告(国際学会見聞録)

    村瀬 潤, 大坪 和香子, 鈴木 研志

    日本微生物生態学会誌 30 (1) 33-33 2015年

    出版者・発行元:日本微生物生態学会

    DOI: 10.20709/jsmeja.30.1_33  

  40. O13-04 酸素存在下において亜酸化窒素(N_2O)発生を抑制する脱窒細菌の単離と特性解析(口頭発表)

    大坪 和香子, 佐賀 武, 鈴木 拓磨, 宮内 啓介, 遠藤 銀朗

    日本微生物生態学会講演要旨集 2014 90-90 2014年

    出版者・発行元:日本微生物生態学会

  41. 国際的な研究キャリアパスを築く : 留学&ライフステージのハードル・メリット・デメリット(2014年度環境微生物系合同大会ランチョンシンポジウム)

    濱村 奈津子, 鮫島 玲子, 大坪 和香子, 三宅 恵子

    日本微生物生態学会講演要旨集 2014 272-273 2014年

    出版者・発行元:日本微生物生態学会

  42. 堆肥化プロセスにおける亜酸化窒素発生に硝化・脱窒細菌が与える影響

    大坪和香子, 進藤絵里香, 上田英代, 上田裕一, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web) 2013 4C11A08 (WEB ONLY) 2013年3月5日

    ISSN:2186-7976

  43. Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2

    Ikeda Ohtsubo Wakako, Morio Miyahara, Sang Wan Kim, Takeshi Yamada, Takeshi Yamada, Masaki Matsuoka, Akira Watanabe, Shinya Fushinobu, Takayoshi Wakagi, Hirofumi Shoun, Keisuke Miyauchi, Ginro Endo

    Seibutsu-kogaku Kaishi 115 37-42 2013年3月1日

    DOI: 10.1016/j.jbiosc.2012.08.015  

    ISSN:0919-3758

    詳細を見る 詳細を閉じる

    In bioaugmentation technology, survival of inoculant in the treatment system is prerequisite but remains to be a crucial hurdle. In this study, we bioaugmented the denitrification tank of a piggery wastewater treatment system with the denitrifying bacterium Pseudomonas stutzeri strain TR2 in two pilot-scale experiments, with the aim of reducing nitrous oxide (N2O), a gas of environmental concern. In the laboratory, strain TR2 grew well and survived with high concentrations of nitrite (5-10 mM) at a wide range of temperatures (28-40°C). In the first augmentation of the pilotscale experiment, strain TR2 inoculated into the denitrification tank with conditions (30°C, ∼0.1 mM nitrite) survived only 2-5 days. In contrast, in the second augmentation with conditions determined to be favorable for the growth of the bacterium in the laboratory (40-45°C, 2-5 mM nitrite), strain TR2 survived longer than 32 days. During the time when the presence of strain TR2 was confirmed by quantitative real-time PCR, N2O emission was maintained at a low level even under nitrite-accumulating conditions in the denitrification and nitrification tanks, which provided indirect evidence that strain TR2 can reduce N2O in the pilot-scale system. Our results documented the effective application of growth conditions favorable for strain TR2 determined in the laboratory to maintain growth and performance of this strain in the pilot-scale reactor system and the decrease of N2O emission as the consequence. © 2012, The Society for Biotechnology, Japan. All rights reserved.

  44. 好気的条件下で脱窒を行う能力を有する堆肥由来細菌の単離と同定

    大坪和香子, 尾形裕明, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web) 2012 2C22A10 (WEB ONLY) 2012年3月5日

    ISSN:2186-7976

  45. 畜産廃棄物のコンポスト化プロセスに存在する脱窒細菌の群集構造の変化

    進藤 絵里香, 大坪 和香子, 宮内 啓介

    東北学院大学工学総合研究所紀要 Bulletin of Research Institute for Engineering and Technology, Tohoku Gakuin University (1) 47-56 2012年3月

    出版者・発行元:東北学院大学工学総合研究所

  46. PE-21 亜酸化窒素還元酵素遺伝子(nosZ)を増幅するPCRプライマー中のミスマッチが脱窒細菌の群集構造解析に与える影響(PE 微生物群集構造,ポスター発表)

    大坪 和香子, 宮内 啓介, 遠藤 銀朗

    日本微生物生態学会講演要旨集 (28) 156-156 2012年

    出版者・発行元:日本微生物生態学会

  47. 耐酸素性脱窒細菌Pseudomonas stutzeri TR2株の脱窒条件における優勢化へ電子受容体が与える影響の評価

    大坪和香子, 白取早恵, 宮内啓介, 遠藤銀朗

    環境バイオテクノロジー学会大会プログラム講演要旨集 44th 49 2011年6月20日

  48. 101p-8 養豚場排水処理残渣の堆肥化プロセスに存在する脱窒細菌群集の定量および系統解析(101p 水圏生態系/群集構造解析,口頭発表)

    大坪 和香子, 進藤 絵里香, 上田 英代, 上田 裕一, 宮内 啓介, 遠藤 銀朗

    日本微生物生態学会講演要旨集 (27) 50-50 2011年

    出版者・発行元:日本微生物生態学会

  49. 亜酸化窒素発生抑止を目的とした特殊脱窒細菌のバイオオーグメンテーションの試み

    大坪和香子, 宮原盛雄, 渡辺昭, 宮内啓介, 若木高善, 祥雲弘文, 遠藤銀朗

    日本農芸化学会大会講演要旨集 2010 251 2010年3月5日

  50. 畜産廃棄物のコンポスト製造過程に関与するアンモニア酸化細菌群の多様性と遷移に関する研究

    荒木伸也, 大坪和香子, 宮内啓介, 遠藤銀朗

    東北学院大学環境防災工学研究所紀要(CD-ROM) (21) ROMBUNNO.2-4 2010年3月

  51. 0605 ゲノム解析に有用なGUIツール : GenomeMatcher(OS44:ゲノム微生物の最前線)

    大坪 嘉行, 大坪 和香子, 永田 裕二, 津田 雅孝

    バイオエンジニアリング講演会講演論文集 2009 (22) 90-90 2010年1月8日

    出版者・発行元:一般社団法人日本機械学会

  52. 高温処理コンポスト化プロセスにおける真核菌類の出現と消長に関する研究

    進藤絵里香, 大坪和香子, 上田裕一, 上田英代, 宮内啓介, 遠藤銀朗

    環境バイオテクノロジー学会大会プログラム講演要旨集 40th 59 2010年

  53. P-30 畜産廃棄物のコンポスト化過程におけるN_2O還元に関与する脱窒細菌群の解析(B.土壌生態系,ポスター発表)

    大坪 和香子, 高橋 諒, 高橋 正行, 進藤 絵里香, 上田 英代, 上田 裕一, 宮内 啓介, 遠藤 銀朗

    日本微生物生態学会講演要旨集 (26) 109-109 2010年

    出版者・発行元:日本微生物生態学会

  54. 1A-5 畜産廃棄物のコンポスト化過程におけるN2O発生に関与する微生物の解析(口頭発表)

    大坪 和香子, 荒木 伸也, 山田 剛史, 上田 英代, 上田 裕一, 宮内 啓介, 遠藤 銀朗

    日本微生物生態学会講演要旨集 (25) 3-3 2009年

    出版者・発行元:日本微生物生態学会

︎全件表示 ︎最初の5件までを表示

書籍等出版物 1

  1. Japan after 3/11: Global Perspectives on the Earthquake, Tsunami, and Fukushima Meltdown

    出島有紀子, 大坪和香子

    University Press of Kentucky 2016年

講演・口頭発表等 22

  1. Lactobacillus plantarum CRL1506における抗ウイルス性イムノジェニクスの解明

    舟橋 龍太郎, 水野 滉也, 友常 加恵, 友清 帝, Md. Aminul Islam、Leonardo Albarracín, 大坪 和香子, 麻生 久, Julio Villena, 北澤 春樹

    日本畜産学会第128回大会 2021年3月29日

  2. ウシ乳腺上皮細胞によるイムノバイオティクスの選抜・評価系構築

    福山 皓太朗, 高木 理宏, Md. Aminul Islam, Leonardo Albarracin, 須田 義人, 大坪 和香子, 野地 智法, 麻生 久, Julio Villena, 北澤 春樹

    日本畜産学会第128回大会 2021年3月29日

  3. Immunododulatory properties and the genomic studies of immunobiotics and immunosynbiotic characterization

    周 冰卉, 犬童 優樹, Leonardo Albarracin, 舛水 由季, Aminul Islam, 大坪 和香子, 麻生 久, Julio Villena, 北澤 春樹

    日本畜産学会第128回大会 2021年3月29日

  4. 同所飼育環境下のウマにおける腸内常在細菌の個体間伝播

    大坪 和香子, Jasper, A.J. Eikelboom, 山川雄大, 米山裕, 西村順子北澤春樹

    日本ウマ科学会第33回学術集会 2020年11月30日

  5. 新規イムノバイオティクス候補となる酪農乳酸菌の 特異的検出・同定のための新規プライマーの開発

    土田咲絵, 大坪和香子, 須田義人, 北澤春樹

    日本乳酸菌学会2020年度大会 2020年11月27日

  6. オキナワモズクフコイダンのプレバイオティクス効果の研究

    有川優希, 大坪和香子

    第 53 回日本無菌生物ノートバイオロジー学会総会 2020年1月25日

  7. Microarray analysis of bovine mammary epithelial cells stimulated with mastitis pathogens

    Michihiro Takagi, Ryoya Komatsu, Julio Villena, Leonardo Albarracin, Md Aminul Islam, Yoshihito Suda, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Hisashi Aso, Haruki Kitazawa

    日本畜産学会第125回大会 2019年3月29日

  8. Construction of porcine immunobiotics library

    Yuhka Nakano, Yuki Masumizu, Ryusuke Ohgi, Leonardo Albarracin, Wakako Ohtsubo, Md Aminul Islam, Yoshihito Suda, Julio Villena, Tomonori Nochi, Haruki Kitazawa

    日本畜産学会第125回大会 2019年3月29日

  9. Selection of wakame utilizing lactobacilli adhering to porcine intestinal mucin and epitheliocytes

    Binghui Zhou, Yuki Masumizu, Yuhka Nakano, Yuhki Indo, Ryoya Komatsu, Md Aminul Islam, Wakako Ohtsubo, Yoshihito Suda, Keiichi Suzuki, Hisashi Aso, Haruki Kitazawa

    日本畜産学会第125回大会 2019年3月29日

  10. Advanced in vitro evaluation system for selecting anti-diarrheal immunobiotics

    Ryoya Komatsu, Hikaru Iida, Leonardo Albarracín, Md Aminul Islam, Wakako Ikeda-Ohtsubo, Hisashi Aso, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Julio Villena, Haruki Kitazawa

    日本畜産学会第125回大会 2019年3月28日

  11. Adhesion ability of bifidobacteria to human intestine

    Binghui Zhou, Noriyuki Iwabuchi, Ohtsubo (Ikeda) Wakako, Jin-zhong Xiao, Tadao Saito, Haruki Kitazawa

    酪農科学シンポジウム2018 2018年9月14日

  12. 海藻多糖のプレバイオティクス機能の探索

    有川優希, 大坪和香子, 木村一雅, 伊波匡彦, 嘉手納 築, 齋藤忠夫, 北澤春樹

    酪農科学シンポジウム2018 2018年9月14日

  13. TLR2 and 4 ligands stimulate inflammation/adipogenesis in porcine adipocytes

    井形愛美ら

    日本畜産学会第124回大会 2018年3月28日

  14. Development of immunobiotic evaluation system on PIE cells stimulated by DSS

    佐藤菜ら

    日本畜産学会第124回大会 2018年3月28日

  15. Research on adhesion property of Bifidobacteria strains to human intestine

    Zhou Binghui, Ikeda-Ohtsubo Wakako, Kitazawa Haruki, Saito Tadao

    日本畜産学会第124回大会 2018年3月28日

  16. ハチミツに含まれるオリゴ糖のビフィズス菌による資化性

    大坪 和香子, 澤田 侑希, 北澤 春樹, 齋藤 忠夫

    日本農芸化学会2018年度大会 2018年3月16日

  17. ハチミツに含まれるオリゴ糖の特徴とプレバイオティクス効果

    澤田侑希, 大坪和香子

    2018年1月26日

  18. ヒト腸管粘膜中に常在する硫酸還元細菌の検出と同定

    日下練介, 大坪和香子

    第51回日本無菌生物ノートバイオロジー学会 2018年1月26日

  19. Exploration Of Sulfate-Reducing Bacteria Colonizing The Intestinal Tracts 国際会議

    大坪和香子, 日下練介

    Microbiology Centennial Symposium, Wageningen University 2017年10月19日

  20. Identification of gut bacteria of leaf beetles feeding on plants with high oxalate contents 国際会議

    大坪和香子, 宮城敦子, Michael Leeら

    International Congress of Bacteriology and Applied Microbiology (IUMS 2017) 2017年7月18日

  21. Identification of sulfate-reducing bacteria in human intestinal mucus 国際会議

    大坪和香子, 日下練介, 北澤春樹, 齋藤忠夫

    Joint Congress of the 19th International Symposium on Gnotobiology (XIX ISG), the 50th Congress of Japanese Association of Germfree Life and Gnotobiology (L JAGG) and the 39th Congress of the Society for Microbial Ecology and Disease (XXXIX SOMED) 2017年6月8日

  22. Lactobacillus bulgaricusのラクトース取込みの対向輸送解析と低ガラクトースヨーグルトの開発

    山田 滉介, 大坪 和香子, 北澤 春樹, 齋藤 忠夫

    日本畜産学会第124回大会 2017年3月28日

︎全件表示 ︎最初の5件までを表示

共同研究・競争的資金等の研究課題 15

  1. 家畜健全育成向上戦略:イムノバイオティクスと自然免疫受容体の腸-肺軸シンフォニー

    北澤 春樹, 西山 啓太, 大坪 和香子, Binghui Zhou, 野地 智法, 竹之内 敬人, 新開 浩樹, 須田 義人

    2023年4月1日 ~ 2027年3月31日

  2. 腸腎連関ネットワークの解明による腎臓病治療法の開発

    阿部 高明, 大坪 和香子, 倉永 英里奈

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:Tohoku University

    2021年4月1日 ~ 2024年3月31日

  3. 内在性抗菌因子PGRPに着目した育種と飼養の抗病性シナジー強化による健全畜産戦略

    北澤 春樹, 大坪 和香子, 麻生 久, 野地 智法, 上西 博英, 宮崎 綾子, 宗田 吉広, 須田 義人

    2019年4月1日 ~ 2023年3月31日

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    本研究は、家畜が有する抗菌因子に着目し、遺伝育種学的手法とイムノバイオティクスの発展的融合により、家畜の抗病性機能強化から抗菌剤に依存しない新たな健全育成技術基盤の創出を目的とする。本年度は、以下の成果が得られた。 【課題1】豚におけるPGLYRPの遺伝子多型と疾病関連形質との関係性解明: 豚PGLYRP1~4について、デュロック種12頭を用いてコード領域その上流5kbの多様性解析を行った。上流配列にはPGLYRP1~4遺伝子でそれぞれ1・10・31・31個のSNPが検出された。また、コード領域にPGLYRP2~4遺伝子でそれぞれ3・7・2個の非同義置換SNPが検出された。 【課題2】PGLYRP増強活性を有するイムノバイオティクスの選抜と抗病性機構解明:豚由来乳酸菌ライブラリーより、PIE細胞を用いたパターン認識受容体を介する抗病性免疫調節機能の観点から4菌株を選抜した。また、イムノバイオティクス候補菌株のPGLYRP発現増強に関する評価を行うと共に、豚由来樹状細胞におけるPGLYRPの発現増強について評価した。 【課題3】HPCD子豚を用いたイムノバイオティクス候補菌株の評価:課題2で選抜された4菌株についてノトバイオート子豚への経口投与を行い、個体レベルでの影響、免疫応答について検討した。また、菌株投与によるロタウイルス感染増殖抑制や症状軽減効果について検討を開始した。 【課題4】一般豚およびPGLYRP選抜豚における健全育成・産肉性向上の実証: LWD豚を供試しイムノバイオティクス候補菌株投与後、血清における生化学検査とサイトカイン濃度、糞便におけるロタウイルス検出、毒素原性大腸菌の検出、腸管組織における免疫関連遺伝子の測定を行うことで、健康性への効果を評価することが可能と考えられた。

  4. オキナワモズクフコイダンによる腸管内酪酸産生細菌の増殖促進作用の解明

    大坪和香子

    提供機関:LOTTE FOUNDATION

    2020年4月 ~ 2023年3月

  5. 家畜対応型の抗病性イムノバオティクス選抜・評価系の確立とその応用戦略

    北澤 春樹, 大坪 和香子, 麻生 久, 齋藤 忠夫

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:Tohoku University

    2016年4月1日 ~ 2020年3月31日

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    世界初となる家畜対応型のイムノバイオティクス(粘膜免疫調節機能性プロバイオティクス)のインビトロ抗病性選抜・評価系を構築し、その発展的利用による家畜健全育成技術基盤を確立した。具体的には、家畜由来腸管上皮細胞株を用いて、細菌・ウイルス等腸管病原体の感染系を確立し、感染防御能を指標とした評価系を構築した。さらに、本評価系により、抗病性イムノバイオティクスを選抜し、インビボ評価により本評価系の有用性を検証することができた。

  6. ヒトおよび動物腸管内に常在する細菌の機能解明

    提供機関:Tohoku University

    2018年9月 ~ 2019年6月

  7. 複合的に炎症性腸疾患を予防する新機能性ヨーグルトの開発 競争的資金

    大坪 和香子, 齋藤 忠夫, 北澤 春樹

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    研究種目:Grant-in-Aid for Challenging Research (Exploratory)

    研究機関:Tohoku University

    2017年6月30日 ~ 2019年3月31日

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    本研究においてヒト腸管試料から見出された高い酪酸酸性能を有するLachnospiraceae科酪酸産生細菌は、海藻多糖を基質として生育することが可能であった。そこで、海藻多糖分解産物であるフコースを資化するビフィズス菌を乳児腸管由来菌株ライブラリから選抜し、フコースを提供する酪酸産生菌と、酢酸を提供するビフィズス菌のクロスフィーディングによる共培養系の条件を検討した。さらに、ヒト腸管ムチンを固定化したマイクロプレートを用いた競合付着阻害試験では、炎症性腸疾患発症に関与することが知られるDesulfovibrio属硫酸還元細菌に対する高い置換・競合能を有するビフィズス菌が見出された。

  8. 日本人腸内フローラによるオキナワモズクフコイダン分解メカニズムの解明 競争的資金

    大坪 和香子

    提供機関:The Towa Foundation for Food Science & Research

    制度名:Research Grant 2018

    2018年6月 ~ 2019年3月

  9. 外来雑草エゾノギシギシを摂食するコガタルリハムシの腸内シュウ酸分解細菌の共生機能の解明 競争的資金

    大坪 和香子

    2017年4月 ~ 2019年3月

  10. 海藻由来食物繊維を利用した新規シンバイオティックヨーグルトの開発研究 競争的資金

    大坪 和香子

    2017年7月 ~ 2018年6月

  11. コガタルリハムシ腸内共生乳酸菌の機能生態の解明

    大坪 和香子, 宮城 敦子

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    研究種目:Grant-in-Aid for Challenging Exploratory Research

    2013年4月1日 ~ 2016年3月31日

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    コガタルリハムシGastrophysa atrocyaneaおよび同属異種のGastrophysa viridulaの幼虫個体から腸内フローラの16S rRNA遺伝子解析およびメタゲノム解析を行った。両種のハムシ腸内において、Rahnella属、Pantoea属、Serratia属の未培養グループの腸内細菌科が優勢化していることが明らかになった。メタゲノムライブラリーから見出されたシュウ酸脱炭酸酵素遺伝子は、既知の腸内細菌科の遺伝子と相同性が最も高かったことから、上述の優勢細菌種がシュウ酸分解能を有し、外来雑草エゾノギシギシを主食とする宿主ハムシのシュウ酸分解に関与している可能性が示唆された。

  12. コガタルリハムシ腸内共生乳酸菌の機能生態の解明 研究課題 競争的資金

    大坪 和香子

    提供機関:Japan Society for the Promotion of Science

    制度名:Grant-in-Aid for Challenging Exploratory Research

    2013年6月 ~ 2016年3月

  13. 畜産廃棄物堆肥中の亜酸化窒素還元除去能を有する脱窒細菌の生態解明 競争的資金

    大坪 和香子

    提供機関:Japan Society for the Promotion of Science

    制度名:Grant-in-Aid for Young Scientists (B)

    2011年4月 ~ 2013年3月

  14. 畜産廃棄物堆肥中の亜酸化窒素還元除去能を有する脱窒細菌の生態解明

    大坪 和香子

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    研究種目:Grant-in-Aid for Young Scientists (B)

    研究機関:Tohoku Gakuin University

    2011年 ~ 2012年

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    本研究では、養豚場の廃水処理プロセスで発生する固形廃棄物の堆肥化実験を行い、堆肥化プロセスにおける N2O 発生時期が硝酸や亜硝酸などの脱窒基質を生成するアンモニア酸化細菌の発生時期と連動することを明らかにした。また、堆積前の堆肥原料の高温前処理が、N2O 発生を低下させ、これが脱窒細菌の N2O 還元能によるものである可能性が示唆された。硝化、脱窒関連遺伝子の系統解析からは、アンモニア酸化細菌や脱窒細菌の群集構造が、堆肥化の進行に従って大きく変化していることが知られた。また、本研究において堆肥から単離された脱窒細菌株は、好気または微好気条件において脱窒を行う能力を有していた。これらの結果は、堆肥化プロセスにおける N2O 発生パターンに、堆肥中のアンモニア酸化細菌と脱窒細菌の群集構造や機能が大きく関与しており、これらを制御することが堆肥化プロセスにおける N2O 発生の削減に重要であると考えられる。

  15. 昆虫モデルを用いた腸内フローラの成熟化の研究

    提供機関:Tohoku University

︎全件表示 ︎最初の5件までを表示

社会貢献活動 2

  1. 【東北学院大学 工学総合研究所公開講座】『微生物』が拓く安全・安心な社会

    2014年10月15日 ~

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    講演「ミクロな動物園 ~腸内微生物の豊かな個性~」

  2. 桜美林大学 2012 年国際シンポジウム — The 2011 Japanese Tsunami: Disaster, Response, and Recovery

    2012年10月19日 ~

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    講演「インターネット時代における親と子:2011年三陸沖地震津波後」