顔写真

ニシタニ ゴウ
西谷 豪
Goh Nishitani
所属
大学院農学研究科 生物生産科学専攻 水圏生産科学講座(生物海洋学分野)
職名
准教授
学位
  • 博士(農学)(京都大学)

  • 修士(農学)(京都大学)

e-Rad 研究者番号
70450781

経歴 1

  • 2021年4月 ~ 継続中
    東北大学 大学院農学研究科・農学部 応用生命科学専攻 環境生命科学講座 生物海洋学分野 准教授

学歴 4

  • 京都大学 農学研究科 応用生物科学

    ~ 1999年

  • 京都大学

    ~ 1999年

  • 広島大学

    ~ 1997年

  • 広島大学 生物生産学部 生物生産

    ~ 1997年

委員歴 3

  • 日本ベントス学会 事務幹事

    2020年4月 ~ 継続中

  • Plankton and Benthos Research 編集委員会 編集委員

    2015年4月 ~ 継続中

  • 日本水産学会 水産環境保全委員会 委員

    2015年3月 ~ 継続中

所属学協会 3

  • 日本ベントス学会

    2019年4月 ~ 継続中

  • 日本プランクトン学会

  • 日本水産学会

研究キーワード 1

  • 有害有毒プランクトン・有用微細藻類・生態学・遺伝子・寄生生物

研究分野 1

  • ライフサイエンス / 生態学、環境学 /

論文 54

  1. Shelters for aquaculture of Octopus sinensis: preferences for gap width and horizontal versus vertical plates

    Yuta Suzumura, Keiji Matsubara, Shunzo Morii, Masami Abe, Ian G. Gleadall, Masazumi Nishikawa, Ayu Katayama, Goh Nishitani, Yu Okawara, Riku Kimura, Nobuhiko Akiyama

    FISHERIES SCIENCE 88 (2) 285-298 2022年3月

    出版者・発行元:SPRINGER JAPAN KK

    DOI: 10.1007/s12562-022-01582-5  

    ISSN:0919-9268

    eISSN:1444-2906

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    It is difficult to keep a large number of cultured octopuses together in the same holding tank due to the high incidence of cannibalism. Appropriate shelters within which the octopuses can more readily avoid their peers are expected to ameliorate this problem. Shelters can be constructed from square plates assembled with appropriate spacing (gap) between them. The purpose of this study was to ascertain the optimum plate orientation and gap width between plates for shelters to maximize the accommodation of octopuses. It was found that the number of shelter occupants increased with the number of gaps available, such that more than half of the individuals in the experimental tank used the same shelter simultaneously. Octopuses tested singly to determine preferences for shelters placed vertically or horizontally preferred the vertical arrangement. Octopuses of more than 300 g tested singly in aquaria given shelters with different gap widths (20-100 mm) were found to not enter shelters with gap widths of 20 or 40 mm. It is concluded that octopus shelters constructed from square vertical plates spaced with gap widths suitable for octopuses are a useful contribution towards maximizing the number of octopuses that can be maintained in culture conditions.

  2. A novel parasite strain of Amoebophrya sp. infecting the toxic dinoflagellate Alexandrium catenella (Group I) and its effect on the host bloom in Osaka Bay, Japan. 国際誌 査読有り

    Goh Nishitani, Keigo Yamamoto, Masaki Nakajima, Yoshiki Shibata, Waka Sato-Okoshi, Mineo Yamaguchi

    Harmful Algae 110 102123-102123 2021年12月

    出版者・発行元:Elsevier BV

    DOI: 10.1016/j.hal.2021.102123  

    ISSN:1568-9883

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    The endoparasitic dinoflagellates belonging to the genus Amoebophrya can infect a broad range of free-living marine dinoflagellates, including harmful/toxic species. The parasite kills its host; the high prevalence of the parasite has been suggested to be a significant factor for the termination of dinoflagellate blooms in marine systems. The issues involved in culturing host-parasite systems have greatly restricted further research on Amoebophrya biology. Here, we established the culture of a novel strain of Amoebophrya sp. ex Alexandrium catenella (Group I) from Osaka Bay, Japan, and studied its genetic diversity, host specificity, and prevalence in the field. Genetic analysis established that the strain we isolated was a novel culture strain infecting A. catenella. Among the host species tested, the Amoebophrya sp. could infect the genera Alexandrium and Prorocentrum in culture, and the infection was also confirmed in the genus Tripos in a field sample. A maximum prevalence of 73% was recorded during the Alexandrium bloom period in Osaka Bay, after which the host cell density rapidly declined. Our results indicated that the existence of the parasite had a significant effect on the dynamics of A. catenella, especially on the termination of the blooms.

  3. Molecular approach for analysis of in situ feeding by the dinoflagellate Noctiluca scintillans 査読有り

    Goh Nishitani, Masaomi Shiromoto, Waka Sato-Okoshi, Akira Ishikawa

    Harmful Algae 99 101928-101928 2020年11月

    出版者・発行元:Elsevier BV

    DOI: 10.1016/j.hal.2020.101928  

    ISSN:1568-9883

  4. Phytoplankton uptake and growth rate in the Japanese egg cockle Fulvia mutica 査読有り

    Nagasoe, S., Hisada, T., Nishitani, G., Takeuchi, H., Sakiyama, K.

    International Aquatic Research 11 359-375 2019年

  5. An effective method for detecting prey DNA from marine dinoflagellates belonging to the genera dinophysis and Phalacroma using a combination of PCR and restriction digestion techniques 査読有り

    Goh Nishitani, Yuki Kosaka, Satoshi Nagai, Yoshihito Takano, Young-Ok Kim, Akira Ishikawa

    Plankton and Benthos Research 13 (2) 90-94 2018年

    出版者・発行元:Plankton Society of Japan

    DOI: 10.3800/pbr.13.90  

    ISSN:1882-627X 1880-8247

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    The marine dinoflagellates species belonging to the genera Dinophysis and Phalacroma have been reported as mixotrophic or heterotrophic actively feeding on planktonic ciliates. However, limited information is available on the identification of ciliate species preyed on by Phalacroma mitra. An effective method is introduced for detecting prey DNA of dinoflagellates belonging to these two genera. Three cells of P. mitra possessing food vacuoles were isolated from natural seawater, and the prey DNA within the vacuoles was analyzed. After the polymerase chain reaction (PCR)-based amplification, a restriction enzyme specific to the DNA of Dinophysis and Phalacroma was used to concentrate the prey DNA. Gene cloning revealed that the undigested PCR products contained DNA of ciliate species (Euplotes sp., Mesodinium rubrum, Spirostrombidium sp., etc.), as well as that of dinoflagellates, cryptophytes, and radiolarians. These results imply that the prey diversity of P. mitra can be traced. Furthermore, this method can provide useful data to reveal novel insights into food webs in the planktonic ecosystem.

  6. A new primer set to amplify the mitochondrial cytochrome c oxidase subunit I (COI) gene in the DHA-rich microalgae, the genus Aurantiochytrium 査読有り

    Goh Nishitani, Masaki Yoshida

    Microbes and Environments 33 227-229 2018年

  7. Seasonal succession of ciliate Mesodinium spp. with red, green, or mixed plastids and their association with cryptophyte prey 査読有り

    Goh Nishitani, Mineo Yamaguchi

    Scientific Reports 8 17189 2018年

  8. And then there was one: Polydora uncinata and Polydora hoplura (Annelida: Spionidae), the problematic polydorid pest species represent a single species 査読有り

    Waka Sato-Okoshi, Hirokazu Abe, Goh Nishitani, Carol A. Simon

    JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM 97 (8) 1675-1684 2017年12月

    出版者・発行元:CAMBRIDGE UNIV PRESS

    DOI: 10.1017/S002531541600093X  

    ISSN:0025-3154

    eISSN:1469-7769

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    It is necessary to monitor shell-associated polydorid worms owing to the risk that they pose to commercially important molluscs. This requires accurate identification, but is often hampered by morphological similarities among species, insufficient type specimens, and abridged species descriptions. Thus morphological characteristics and molecular sequences of the most harmful polydorid species, Polydora uncinata from Japan and Australia and Polydora hoplura from South Africa were compared to determine whether they represent two morphologically similar, but genetically distinct species, or a single species. A wide range of morphological variation (with respect to size, length of caruncle and pigmentation patterns) was observed in each species and population, and their variations largely overlapped and revealed them to be at the intraspecific level of a single species. This was confirmed by gene sequences of nuclear 18S and 28S rRNA that were completely identical for P. uncinata and P. hoplura. The mitochondrial 16S rRNA and cyt b gene analyses also showed no genetic differences between these two species. The tree topology of the mitochondrial cyt b gene did not reflect geographic differences but instead suggests artificial transportation of the species. We recommend the synonymization of P. uncinata with P. hoplura.

  9. Germination fluctuation of toxic Alexandrium fundyense and A. pacificum cysts and the relationship with bloom occurrences in Kesennuma Bay, Japan 査読有り

    Masafumi Natsuike, Katsuhide Yokoyama, Goh Nishitani, Yuichiro Yamada, Ikuo Yoshinaga, Akira Ishikawa

    HARMFUL ALGAE 62 52-59 2017年2月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/j.hal.2016.11.018  

    ISSN:1568-9883

    eISSN:1878-1470

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    While cyst germination may be an important factor for the initiation of harmful/toxic blooms, assessments of the fluctuation in phytoplankton cyst germination, from bottom sediments to water columns, are rare in situ due to lack of technology that can detect germinated cells in natural bottom sediments. This study introduces a simple mesocosm method, modeled after previous in situ methods, to measure the germination of plankton resting stage cells. Using this method, seasonal changes in germination fluxes of toxic dinoflagellates resting cysts, specifically Alexandrium fundyense (A. tamarense species complex Group I) and A. pacificurn (A. tamarense species complex Group IV), were investigated at a fixed station in Kesennuma Bay, northeast Japan, from April 2014 to April 2015. This investigation was conducted in addition to the typical samplings of seawater and bottom sediments to detect the dinoflagellates vegetative cells and resting cysts. Bloom occurrences of A. fundyense were observed June 2014 and February 2015 with maximum cell densities reaching 3.6 x 10(6) cells m(-2) and 1.4 x 10(7) cells m(-2), respectively. The maximum germination fluxes of A. fundyense cysts occurred in April 2014 and December 2014 and were 9.3 x 10(3) cells m(-2) day(-1) and 1.4 x 10(4) cells m(-2) day(-1), respectively. For A. pacificum, the highest cell density was 73 x 10(7) cells m(-2) during the month of August, and the maximum germination fluxes occurred in July and August, reaching 5.8 x 10(2) cells m(-2) day(-1). Thus, this study revealed the seasonal dynamics of A. fundyense and A. pacificurn cyst germination and their bloom occurrences in the water column. Blooms occurred one to two months after peak germination, which strongly suggests that both the formation of the initial population by cyst germination and its continuous growth in the water column most likely contributed to toxic bloom occurrences of A. fundyense and A. pacific -um in the bay. (C) 2016 Elsevier B.V. All rights reserved.

  10. Life cycle of the suctorian ciliate Ephelota plana attached to the krill Euphausia pacifica 査読有り

    Yoshinari Endo, Daiki Fujii, Goh Nishitani, Peter H. Wiebe

    JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY 486 368-372 2017年1月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/j.jembe.2016.11.003  

    ISSN:0022-0981

    eISSN:1879-1697

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    The hypothesis that life cycle of an epibiotic suctorian ciliate Ephelota piano is adapted to the molt cycle of the krill Euphausia pacifica collected in Saanich Inlet, Canada was evaluated. The infestation prevalence of E. planet and the number of individuals attached increased from postmolt stage to premolt stage of E. pacifica, and concurrently cell growth of E. plana was observed. Budding individuals of E. plana first appeared at early premolt stage and increased to 21% at late premolt stage. Thus the life cycle of E. piano seems to be adapted to the molt cycle of E. pacifica. (C) 2016 Elsevier B.V. All rights reserved.

  11. Early feeding by cultured paralarvae of Octopus sinensis: comparison of survival, fatty acid and amino acid profiles using two different strains of Artemia 査読有り

    KEIJI MATSUBARA, SHUNZO MORII, MASAMI ABE, GOH NISHITANI, MASAZUMI NISHIKAWA, NOBUHIKO AKIYAMA, IAN G. GLEADALL

    Asian Fisheries Science 29 57-64 2016年3月

  12. Identification of okadaic acid binding protein 2 in reconstituted sponge cell clusters from Halichondria okadai and its contribution to the detoxification of okadaic acid 査読有り

    Keiichi Konoki, Kayo Okada, Mami Kohama, Hiroki Matsuura, Kaori Saito, Yuko Cho, Goh Nishitani, Tomofumi Miyamoto, Seketsu Fukuzawa, Kazuo Tachibana, Mari Yotsu-Yamashita

    TOXICON 108 38-45 2015年12月

    出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD

    DOI: 10.1016/j.toxicon.2015.09.026  

    ISSN:0041-0101

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    Okadaic acid (OA) and OA binding protein 2 (OABP2) were previously isolated from the marine sponge Halichondria okadai. Because the amino acid sequence of OABP2 is completely different from that of protein phosphatase 2A, a well-known target of OA, we have been investigating the production and function of OABP2. In the present study, we hypothesized that OABP2 plays a role in the detoxification of OA in H. okadai and that the OA concentrations are in proportional to the OABP2 concentrations in the sponge specimens. Based on the OA concentrations and the OABP2 concentrations in the sponge specimens collected in various places and in different seasons, however, we could not determine a positive correlation between OA and OABP2. We then attempted to determine distribution of OA and OABP2 in the sponge specimen. When the mixture of dissociated sponge cells and symbiotic species were separated with various pore-sized nylon meshes, most of the OA and OABP2 was detected from the same 0-10 mu m fraction. Next, when sponge cell clusters were prepared from a mixture of dissociated sponge cells and symbiotic species in the presence of penicillin and streptomycin, we identified the 18S rDNA of H. okadai and the gene of OABP2 in the analysis of genomic DNA but could not detect OA by LC-MS/MS. We thus concluded that the sponge cells express OABP2, and that OA was not apparently present in the sponge cells but could be colocalized with OABP2 in the sponge cells at a concentration less than the limit of detection. (C) 2015 Elsevier Ltd. All rights reserved.

  13. Distribution patterns of Alexandrium vegetative cells and resting cysts, and paralytic shellfish poisoning in Kesennuma Bay, after the Great East Japan Earthquake 査読有り

    Tetsuroh Ishikawa, Keisaku Kusaka, Akio Oshino, Goh Nishitani, Takashi Kamiyama

    NIPPON SUISAN GAKKAISHI 81 (2) 256-266 2015年3月

    出版者・発行元:JAPANESE SOC FISHERIES SCIENCE

    ISSN:0021-5392

    eISSN:1349-998X

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    In 2013, paralytic shellfish poisoning (PSP) occurred in Kesennuma Bay, northern Japan, after an interval of 24 years. From April to early May, Alexandrium tamarense increased in the inner part of the bay (up to 136,200 cells/L), expanded to the whole of the bay, and caused PSP. In contrast, A. catenella increased in early September (up to 1,310 cells/L) in the center of the bay, but did not cause PSP. Resting cysts of A. catenella tamarense ranged from 30 to 6,383 cysts/cm(3), and dominated in the inner part of the bay. A meta-analysis of temporal trends in the density of A. catenella/tamarense cysts in Kesennuma Bay revealed that the cysts in the surface bottom sediment drastically increased after the Great East Japan Earthquake. High PSP toxicity was observed in four bivalves, Chlamys farreri (130 MU/g), Mytilus galloprovincialis (110 MU/g), Ruditapes philippinarum (74 MU/g), and Mizuhopecten yessoensis (66 MU/g).

  14. Morphological, Developmental, and Ecological Characteristics of the Suctorian Ciliate Ephelota gigantea (Ciliophora, Phyllopharyngea, Ephelotidae) Found on Cultured Wakame Seaweed in Northeastern Japan 査読有り

    Yuma Sato, Taka-aki Muto, Yoshinari Endo, Toshimasa Kobayashi, Nobuyuki Nakano, Hiroyasu Sato, Goh Nishitani, Waka Sato-Okoshi

    ACTA PROTOZOOLOGICA 54 (4) 295-303 2015年

    出版者・発行元:JAGIELLONIAN UNIV, INST ENVIRONMENTAL SCIENCES

    DOI: 10.4467/16890027AP.15.025.3539  

    ISSN:0065-1583

    eISSN:1689-0027

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    Wakame seaweed is an important aquatic resource in Iwate Prefecture. However, a suctorian Ephelota gigantea sometimes causes great damage to wakame culture. Since little is known about the biological characteristics of E. gigantea, its detailed morphology and temporal change of biological characteristics during the 2010 culture season were investigated. Scanning electron microscope observations showed that E. gigantea had different striation patterns on the stalk; there was a swell made of cement by which the stalk was attached to wakame firmly; and the buds had cilia arranged in concentric circles about a ring in the center of the ventral side. A suctorian parasite was found to infect E. gigantea, and the infection seemed to have decreased drastically the attached density of E. gigantea on wakame. Cell size of parasite-infected E. gigantea individuals was larger than that of uninfected individuals, probably because larger E. gigantea has larger surface area for attachment of the parasite. Cyst formation or conjugating individuals were not observed.

  15. 東日本大震災後の宮城県気仙沼湾におけるAlexandrium属の栄養細胞とシストの分布パターンおよび二枚貝類の毒化 査読有り

    石川哲郎, 日下啓作, 押野明夫, 西谷豪, 神山孝史

    日本水産学会誌 81 (2) 256-266 2015年

    出版者・発行元:None

    DOI: 10.2331/suisan.81.256  

    ISSN:1349-998X 0021-5392

  16. 東日本大震災発生後の東日本・北日本太平洋岸における麻痺性貝毒原因渦鞭毛藻Alexandrium tamarenseの出現状況(総説)

    夏池真史, 西谷 豪, 山田雄一郎, 横山勝英, 吉永郁生

    月刊海洋 46 62-71 2014年12月

  17. Swimming behavior of the spoon worm Urechis unicinctus (Annelida, Echiura) 査読有り

    Hirokazu Abe, Waka Sato-Okoshi, Masaatsu Tanaka, Kenji Okoshi, Wataru Teramoto, Tomohiko Kondoh, Goh Nishitani, Yoshinari Endo

    ZOOLOGY 117 (3) 216-223 2014年6月

    出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG

    DOI: 10.1016/j.zool.2013.12.001  

    ISSN:0944-2006

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    Large numbers of swimming and stranding Urechis unicinctus were observed at night during low tide in Sasuhama, Miyagi Prefecture, northeastern Japan, during the periods from January to February in 2012 and 2013. Worms did not drift passively but swam actively, therefore hinting at a certain purpose for such behavior. As trochophore larvae of U. unicinctus were observed to occur simultaneously in the plankton, we infer the possibility that this is an event of reproductive swarming. Anatomical observations of both swimming and stranding U. unicinctus showed that none of the specimens had gametes, which may suggest that these were completely spent after spawning. Urechis unicinctus seemed to begin swimming after dusk and the observed swimming behavior occurred during the evening ebb tide throughout the night low tide during winter time. Stranding U. unicinctus have long been known in Japan and have been attributed to sea storms. The present study shows for the first time the possibility that U. unicinctus swims in order to reproduce at night and that this swimming behavior is closely linked to the stranding of U. unicinctus individuals. (C) 2014 Elsevier GmbH. All rights reserved.

  18. Vertical distribution and migration of planktonic polychaete larvae in Onagawa Bay, north-eastern Japan 査読有り

    Hirokazu Abe, Waka Sato-Okoshi, Goh Nishitani, Yoshinari Endo

    Memoirs of Museum Victoria 71 1-9 2014年

    出版者・発行元:Museum Victoria

    DOI: 10.24199/j.mmv.2014.71.01  

    ISSN:1447-2554 1447-2546

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    The planktonic larvae of polychaetes are one of the most numerous and diverse groups in coastal zooplankton however, little is known about their larval dynamics and the factors that affect their vertical distribution. We investigated the vertical distribution and migration of planktonic polychaete larvae in Onagawa Bay, north-eastern Japan, particularly focusing on the dominant spionid larvae. In total, 14 families of planktonic polychaete larvae and 14 species or genera of spionid larvae were identified during our study. Their density greatly fluctuated according to season and depth, with the polychaete larvae generally distributed in the lower layers of the water column. Furthermore, trends in vertical distribution of spionid larvae varied between species. In winter and spring, larvae of Polydora onagawaensis were the most prevalent, with a wide range in vertical distribution. In summer and autumn, larvae of Pseudopolydora achaeta and Prionospio spp. were the most prevalent spionid larvae and were primarily distributed in the lower layers of the water column. Trends in larval vertical distribution varied as a result of differences in adult habitat these variations would enable the larvae to efficiently recruit into their appropriate adult habitats. Spionid larvae did not show diel vertical migration. Larvae of two spionid taxa, Pseudopolydora achaeta and Prionospio spp., exhibited tidal vertical migration, with larvae appearing to avoid dispersal by moving to slower-flowing deeper water during flood and ebb tides. Although many previous studies assume that, because of their limited swimming capacity, polychaete larvae are passively dispersed within the water column, this study indicates that polychaete larvae can control their vertical distribution to some extent, and this smallscale vertical migration may be important as a retention mechanism for polychaete larvae.

  19. 2013年に気仙沼舞根湾海域で発生した有害有毒プランクトン

    西谷 豪, 山田 雄一郎, 長坂 翔子, 横山 勝英, 夏池 真史, 吉永 郁生

    海洋と生物 209 568-574 2013年12月

  20. Feeding and Growth Characteristics of a Diatom-Feeding Flagellate Isolated from the Bottom Sediment of Onagawa Bay, Northeastern Japan 査読有り

    Hiromasa Ohno, Yoshinari Endo, Waka Sato-Okoshi, Goh Nishitani

    Open Journal of Marine Science 3 9-14 2013年6月

  21. Morphology, 18S rRNA gene sequence and life history of a new Polydora species (Polychaeta: Spionidae) from northeastern Japan 査読有り

    Wataru Teramoto, Waka Sato-Okoshi, Hirokazu Abe, Goh Nishitani, Yoshinari Endo

    AQUATIC BIOLOGY 18 (1) 31-45 2013年

    出版者・発行元:INTER-RESEARCH

    DOI: 10.3354/ab00485  

    ISSN:1864-7790

    eISSN:1864-7782

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    A new species of spionid polychaete, Polydora onagawaensis, is described from mollusk shells in Pacific waters of northeastern Japan. Its nuclear 18S rRNA gene sequence as well as its morphology, reproductive features, life history and infestation characteristics are reported. Polydora onagawaensis sp. nov. belongs to the Polydora ciliata/websteri group and has a moderate size and variable black pigmentation on the palps and body. Up to 115 worms were found boring in a single scallop shell from suspended cultures in Onagawa Bay, with significantly higher numbers in the right than in the left valve. Females repeatedly deposited a string of egg capsules from around October to June (seawater temperature was below 15 degrees C). The larvae developed inside the egg capsules for 2 wk (10 degrees C, laboratory conditions), until the 3-chaetiger stage, before being released as planktonic larvae. The main spawning occurred in December, recruitment onto the shells increased after January, and most large worms disappeared between July and October. Thus, the estimated life span is around 1.5 yr after settlement. Details on biology and gene information not only contribute to distinguishing the species from other polydorids similar in morphology, but also allow control of polydorid infestation in mollusk aquaculture.

  22. 気仙沼舞根湾の植物プランクトンの動態

    西谷 豪, 山本 光夫, 夏池 真史, 劉 丹, 吉永 郁生

    海洋と生物 34 (6) 545-555 2012年12月

  23. 個体識別による有害赤潮藻Cochlodinium polykrikoidesの移送経路の解明 査読有り

    長井 敏, 西谷 豪

    DNA鑑定 4 49-57 2012年11月1日

    出版者・発行元:DNA鑑定学会

    ISSN:2185-078X

  24. Multiple Plastids Collected by the Dinoflagellate Dinophysis mitra through Kleptoplastidy 査読有り

    Goh Nishitani, Satoshi Nagai, Shiho Hayakawa, Yuki Kosaka, Kiyonari Sakurada, Takashi Kamiyama, Takashi Gojobori

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY 78 (3) 813-821 2012年2月

    出版者・発行元:AMER SOC MICROBIOLOGY

    DOI: 10.1128/AEM.06544-11  

    ISSN:0099-2240

    eISSN:1098-5336

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    Kleptoplastidy is the retention of plastids obtained from ingested algal prey, which may remain temporarily functional and be used for photosynthesis by the predator. We showed that the marine dinoflagellate Dinophysis mitra has great kleptoplastid diversity. We obtained 308 plastid rbcL sequences by gene cloning from 14 D. mitra cells and 102 operational taxonomic units (OTUs). Most sequences were new in the genetic database and positioned within Haptophyceae (227 sequences [73.7%], 80 OTUs [78.4%]), particularly within the genus Chrysochromulina. Others were closely related to Prasinophyceae (16 sequences [5.2%], 5 OTUs [4.9%]), Dictyochophyceae (14 sequences [4.5%], 5 OTUs [4.9%]), Pelagophyceae (14 sequences [4.5%], 1 OTU [1.0%]), Bolidophyceae (3 sequences [1.0%], 1 OTU [1.0%]), and Bacillariophyceae (1 sequence [0.3%], 1 OTU [1.0%]); however, 33 sequences (10.8%) as 9 OTUs (8.8%) were not closely clustered with any particular group. Only six sequences were identical to those of Chrysochromulina simplex, Chrysochromulina hirta, Chrysochromulina sp. TKB8936, Micromonas pusilla NEPCC29, Micromonas pusilla CCMP491, and an unidentified diatom. Thus, we detected >100 different plastid sequences from 14 D. mitra cells, strongly suggesting kleptoplastidy and the need for mixotrophic prey such as Laboea, Tontonia, and Strombidium-like ciliates, which retain numerous symbiotic plastids from different origins, for propagation and plastid sequestration.

  25. Direct detection of harmful algae from the oyster spat and live fish transporting trailers. 査読有り

    Matsuyama Y, Nishitani G, Nagai S

    Proceedings of 13th International Conference on Harmful Algae 2010年10月

  26. Genetic analyses of the cryptomonad nucleomorph in natural Myrionecta rubra cells reveal a simple lineage of Dinophysis kleptoplastids. 査読有り

    Nishitani G, Nagai S, Baba K, Kiyokawa S, Kosaka Y, Miyamura K, Nishikawa T, Sakurada K, Shinada A, Kamiyama T

    Proceedings of 13th International Conference on Harmful Algae 2010年10月

  27. Genetic structure of Asian geographic populations in Alexandrium catenella (Dinophyceae): a study using microsatellite markers. 査読有り

    Genovesi B, Nishitani G, Wang J, Masseret E, Grzebyk D, Berrebi P, Nagai S

    Proceedings of 13th International Conference on Harmful Algae 2010年10月

  28. High-level congruence of Myrionecta rubra prey and Dinophysis species plastid identities as revealed by genetic analyses of isolates from Japanese coastal waters. 国際誌 査読有り

    Goh Nishitani, Satoshi Nagai, Katsuhisa Baba, Susumu Kiyokawa, Yuki Kosaka, Kazuyoshi Miyamura, Tetsuya Nishikawa, Kiyonari Sakurada, Akiyoshi Shinada, Takashi Kamiyama

    Applied and environmental microbiology 76 (9) 2791-8 2010年5月

    DOI: 10.1128/AEM.02566-09  

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    We analyzed cryptophyte nucleomorph 18S rRNA gene sequences retained in natural Myrionecta rubra cells and plastid 16S rRNA gene and psbA sequences retained in natural cells of several Dinophysis species collected from Japanese coastal waters. A total of 715 nucleomorph sequences obtained from 134 M. rubra cells and 564 plastid 16S rRNA gene and 355 psbA sequences from 71 Dinophysis cells were determined. Almost all sequences in M. rubra and Dinophysis spp. were identical to those of Teleaulax amphioxeia, suggesting that M. rubra in Japanese coastal waters preferentially ingest T. amphioxeia. The remaining sequences were closely related to those of Geminigera cryophila and Teleaulax acuta. Interestingly, 37 plastid 16S rRNA gene sequences, which were different from T. amphioxeia and amplified from Dinophysis acuminata and Dinophysis norvegica cells, were identical to the sequence of a D. acuminata cell found in the Greenland Sea, suggesting that a widely distributed and unknown cryptophyte species is also preyed upon by M. rubra and subsequently sequestered by Dinophysis. To confirm the reliability of molecular identification of the cryptophyte Teleaulax species detected from M. rubra and Dinophysis cells, the nucleomorph and plastid genes of Teleaulax species isolated from seawaters were also analyzed. Of 19 isolates, 16 and 3 clonal strains were identified as T. amphioxeia and T. acuta, respectively, and no sequence variation was confirmed within species. T. amphioxeia is probably the primary source of prey for M. rubra in Japanese coastal waters. An unknown cryptophyte may serve as an additional source, depending on localities and seasons.

  29. Development of compound microsatellite markers in the marine phytoplankton Alexandrium tamiyavanichii (Dinophyceae) 査読有り

    Goh Nishitani, Satoshi Nagai, Yuichi Kotani

    CONSERVATION GENETICS 10 (5) 1561-1563 2009年10月

    出版者・発行元:SPRINGER

    DOI: 10.1007/s10592-008-9793-9  

    ISSN:1566-0621

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    We isolated 24 polymorphic microsatellite markers from the toxic dinoflagellate A. tamiyavanichii. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from 2 to 6, and the estimate of gene diversity was from 0.269 to 0.739 across the 24 microsatellites. We consider that these loci have a potentially to reveal the genetic structure and gene flow among A. tamiyavanichii populations.

  30. Genetic structuring and transfer of marine dinoflagellate Cochlodinium polykrikoides in Japanese and Korean coastal waters revealed by microsatellites 査読有り

    S. Nagai, G. Nishitani, S. Sakamoto, T. Sugaya, C. K. Lee, C. H. Kim, S. Itakura, M. Yamaguchi

    MOLECULAR ECOLOGY 18 (11) 2337-2352 2009年6月

    出版者・発行元:WILEY-BLACKWELL

    DOI: 10.1111/j.1365-294X.2009.04193.x  

    ISSN:0962-1083

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    To determine the process of population expansion and ascertain the origin of the Sea of Japan population, in a noxious red tide forming dinoflagellate Cochlodinium polykrikoides, 13 samples, isolated from 11 different localities in Japanese and Korean coasts, were analysed using 10 polymorphic microsatellites. Analyses by nonmetric multidimensional scaling plots of pairwise F(ST), global amova, and genetic admixture analysis identified three clusters - the Sea of Japan populations, Yatsushiro Sea (Kumamoto Pref.) populations, and other populations - indicating genetic structuring of the 13 samples into three distinct populations. In the proportion of shared alleles by pairwise individuals (P(SAxy)) analyses between the Sea of Japan and the other samples, P(SAxy) was extremely low compared with that among the Sea of Japan or among other samples, indicating that a large genetic barrier has occurred between the populations. No significant relationship of isolation-by-distance patterns and almost no genetic distance were detected between pairwise samples of the Sea of Japan, although there is a maximal distance of > 600 km between samples. In addition, P(SAxy) data among the samples were extremely high compared with those among other samples, clearly showing that a large-scale transfer from west to east has occurred via the Tushima Warm Current. In the P(SAxy) data of the Seto Inland Sea and Pacific samples, individuals showing relatively high P(SAxy) were concentrated in the three areas of Nagasaki, Harima, and Mie, suggesting that frequent transfer may have occurred by human-assisted dispersal, although Nagasaki and Mie are separated by a distance of approximately 700 km.

  31. Development of microsatellite markers in the marine phytoplankton Karenia mikimotoi (Dinophyceae) 査読有り

    Goh Nishitani, Satoshi Nagai, Chunlan Lian, Sanae Sakiyama, Akihiko Oohashi, Kazuyoshi Miyamura

    CONSERVATION GENETICS 10 (3) 713-715 2009年6月

    出版者・発行元:SPRINGER

    DOI: 10.1007/s10592-008-9628-8  

    ISSN:1566-0621

    eISSN:1572-9737

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    The marine phytoplankton, Karenia mikimotoi, causes severe red tides which are associated with mass mortality of marine fish, and have expanded their distributions in the coastal waters of western Japan. To assess the dispersal mechanism, a population genetic study using highly polymorphic genetic markers is one of the crucial approaches. Here we developed 12 polymorphic microsatellite markers from K. mikimotoi. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from 5 to 23, and the estimate of gene diversity was from 0.551 to 0.933 across the 12 microsatellites. We consider these loci potentially useful for detailing the genetic structure and gene flow among K. mikimotoi populations.

  32. Genetic structuring and transfer of marine dinoflagellate Cochlodinium polykrikoides in Japanese and Korean coastal waters revealed by microsatellites 査読有り

    S. Nagai, G. Nishitani, S. Sakamoto, T. Sugaya, C. K. Lee, C. H. Kim, S. Itakura, M. Yamaguchi

    MOLECULAR ECOLOGY 18 (11) 2337-2352 2009年6月

    出版者・発行元:WILEY-BLACKWELL

    DOI: 10.1111/j.1365-294X.2009.04193.x  

    ISSN:0962-1083

    詳細を見る 詳細を閉じる

    To determine the process of population expansion and ascertain the origin of the Sea of Japan population, in a noxious red tide forming dinoflagellate Cochlodinium polykrikoides, 13 samples, isolated from 11 different localities in Japanese and Korean coasts, were analysed using 10 polymorphic microsatellites. Analyses by nonmetric multidimensional scaling plots of pairwise F(ST), global amova, and genetic admixture analysis identified three clusters - the Sea of Japan populations, Yatsushiro Sea (Kumamoto Pref.) populations, and other populations - indicating genetic structuring of the 13 samples into three distinct populations. In the proportion of shared alleles by pairwise individuals (P(SAxy)) analyses between the Sea of Japan and the other samples, P(SAxy) was extremely low compared with that among the Sea of Japan or among other samples, indicating that a large genetic barrier has occurred between the populations. No significant relationship of isolation-by-distance patterns and almost no genetic distance were detected between pairwise samples of the Sea of Japan, although there is a maximal distance of > 600 km between samples. In addition, P(SAxy) data among the samples were extremely high compared with those among other samples, clearly showing that a large-scale transfer from west to east has occurred via the Tushima Warm Current. In the P(SAxy) data of the Seto Inland Sea and Pacific samples, individuals showing relatively high P(SAxy) were concentrated in the three areas of Nagasaki, Harima, and Mie, suggesting that frequent transfer may have occurred by human-assisted dispersal, although Nagasaki and Mie are separated by a distance of approximately 700 km.

  33. Encystment and excystment under laboratory conditions of the nontoxic dinoflagellate Alexandrium fraterculus (Dinophyceae) isolated from the Seto Inland Sea, Japan 査読有り

    Satoshi Nagai, Goh Nishitani, Yoshihito Takano, Makoto Yoshida, Haruyoshi Takayama

    PHYCOLOGIA 48 (3) 177-185 2009年5月

    出版者・発行元:INT PHYCOLOGICAL SOC

    DOI: 10.2216/08-43.1  

    ISSN:0031-8884

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    The sexuality (i.e. encystment and excystment) of the nontoxic dinoflagellate Alexandrium fraterculus, isolated from the Seto Inland Sea, Japan, was clarified for the first time under laboratory conditions. Sexual reproduction was by conjugation of isogametes, and plasmogamy was completed in 25-60 min after the conjugation started and produced a planozygote with one transverse and two longitudinal flagella, then a cyst was formed. Cysts were round or elliptical. The cyst size was 37.5-50.0 mu m in diameter. The surface of cysts was smooth, and there was no paratabulation. Encystment through sexual reproduction was observed in two of 28 pairs, which included seven self-crossings, by use of seven nonaxenic clonal strains. No planozygote formation or encystment was found in any of the self-crossings, indicating that this species is heterothallic. Successful excystment was observed when the cysts, which had been preserved at 10 degrees C in the dark for 3 mo, were incubated under light conditions at 25 degrees C, thus showing that this species has a similar life cycle as reported in A. tamarensel catenella tamiyavanichii.

  34. Development of compound microsatellite markers in red-tide-causing dinoflagellate Akashiwo sanguinea (Dinophyceae) 査読有り

    S-Y. Cho, S. Nagai, G. Nishitani, M-S. Han

    MOLECULAR ECOLOGY RESOURCES 9 (3) 915-917 2009年5月

    出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC

    DOI: 10.1111/j.1755-0998.2008.02474.x  

    ISSN:1755-098X

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    We isolated 13 polymorphic microsatellites from the red-tide causing dinoflagellate Akashiwo sanguinea. These loci were highly variable, with between 2 and 10 alleles per locus, and estimated gene diversity ranging from 0.08 to 0.82. These loci have the potential to reveal genetic structure and estimate gene flow among A. sanguinea populations.

  35. マイクロサテライトによるCochlodinium polykrikoidesの個体群構造解析 査読有り

    長井 敏, 西谷 豪

    日本プランクトン学会報 56 47-51 2009年

  36. Alexandrium catenella in Thau lagoon (France) is not a recent introduction from Asia? 査読有り

    Genovesi B, Reynaud N, Nishitani G, Wang J, Masseret E, Berrebi P, Nagai S

    Harmful Algal News 40 1-3 2009年

  37. Predation by the toxic dinoflagellate Dinophysis fortii on the ciliate Myrionecta rubra and observation of sequestration of ciliate chloroplasts 査読有り

    Satoshi Nagai, Goh Nitshitani, Yuji Tomaru, Sanae Sakiyama, Takashi Kamiyama

    JOURNAL OF PHYCOLOGY 44 (4) 909-922 2008年8月

    出版者・発行元:WILEY-BLACKWELL

    DOI: 10.1111/j.1529-8817.2008.00544.x  

    ISSN:0022-3646

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    This is the first report of the propagation of the toxic dinoflagellate Dinophysis fortii Pavill. under laboratory conditions when fed on the marine ciliate Myrionecta rubra grown with the cryptophyte Teleaulax amphioxeia (W. Conrad) D. R. A. Hill. In contrast, reduced growth of D. fortii (max. of 3-4 divisions) and formation of small cells were observed in the absence of the ciliate or when provided with T. amphioxeia only as prey, showing that D. fortii cannot utilize T. amphioxeia as prey. In the TEM observation of D. fortii cells, which had fully fed on the ciliate prey, well-developed chloroplasts (5-12 mu m in length) were seen and three thylakoids were usually arranged in most of the chloroplasts observed, but chloroplasts having two thylakoids were sometimes confirmed. In cells starved for 4 weeks, decrease of chloroplast numbers and disappearance of large chloroplasts were observed, and only a few small chloroplasts (0.5-2 mu m in length) remained in the marginal regions. In the observation of the sequestration process of the chloroplasts ingested from M. rubra by D. fortii, within 15 min after D. fortii captured M. rubra, incorporation of almost all of the chloroplasts was observed, while most of the other cell contents still remained in the M. rubra cell. After that, dispersion of the ingested chloroplasts toward the marginal regions was confirmed, suggesting that chloroplasts of M. rubra are ingested and dispersed in D. fortii cells in advance of the ingestion of the other cell contents to prevent them from being digested in food vacuoles. The ingested chloroplasts can also function as kleptoplastids.

  38. Morphology and germination characteristics of the cysts of Chattonella ovata (Raphidophyceae), a novel red tide flagellate in the Seto Inland Sea, Japan 査読有り

    Mineo Yamaguchi, Haruo Yamaguchi, Goh Nishitani, Setsuko Sakamoto, Shigeru Itakura

    HARMFUL ALGAE 7 (4) 459-463 2008年6月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/j.hal.2007.10.002  

    ISSN:1568-9883

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    To elucidate the mechanism of bloom outbreaks of Chattonella ovata (Raphidophyceae), we investigated the cysts of C ovata and succeeded in finding them from the bottom sediments of Hiroshima Bay. The morphology of the cysts was mostly hemispherical in shape, with a diameter of ca. 30 mu m and height of ca. 20 mu m. The cysts were usually adhering to solid materials, such as diatom frustules, yellow-greenish in color and had several dark brown grains. The cyst wall was smooth and had no ornamentation. Because the morphological characteristic of the cysts was in general agreement with those of Chattonella antiqua and Chattonella marina, it was difficult to differentiate the cysts of these three species. Germination of the cysts of C ovata was observed at temperatures from 17.5 to 30 degrees C, but not at 15 degrees C or below. The number of the germinated cysts increased with increasing temperature and the optimum temperature for germination was 30 degrees C. Although cysts of C antiqua and C marina germinated at temperatures from 15 to 30 degrees C, optimum temperature of germination was 22.5 degrees C. The lower limit and optimum temperatures for germination of C. ovata cysts was higher than for C. antiqua and C. marina. The role of cysts in the population dynamics of C. ovata is discussed. (C) 2007 Elsevier B.V. All rights reserved.

  39. マイクロサテライト多型による有害赤潮藻 Cochlodinium polykrikoides の個体群構造解析 査読有り

    西谷 豪, 長井 敏, 坂本 節子, 練 春蘭, LEE Chang Kyu, 氏 良介, 斉藤 義昭, 櫻田 清成, 杉原 志貴, 中西 克之, 西川 哲也, 藤原 正夢, 村田 圭助, 林 芳弘, 山砥 稔文, 長副 聡, 板倉 茂, 山口 峰生

    DNA多型 = DNA polymorphism 16 140-144 2008年5月30日

  40. Successful cultivation of the toxic dinoflagellate Dinophysis caudata (Dinophyceae) 査読有り

    Goh Nishitani, Satoshi Nagai, Sanae Sakiyama, Takashi Kamiyama

    Plankton and Benthos Research 3 (2) 78-85 2008年5月

    DOI: 10.3800/pbr.3.78  

    ISSN:1880-8247

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    Recently, Park et al. (2006) succeeded in cultivating the toxic dinoflagellate Dinophysis acuminata and maintaining them by feeding the ciliate Myrionecta rubra grown with a cryptophyte Teleaulax sp. After this report, the present study is the second report of propagation of a Dinophysis species (Dinophysis caudata) under laboratory conditions and describes the maintenance of several clonal strains kept at high abundance (&gt 5,000 cells mL -1) for a relatively long period (&gt 4 months) when fed on M. rubra with the addition of Teleaulax amphioxeia. We confirmed that D. caudata swam actively around its ciliate prey and inserted its peduncle (feeding tube) into the ciliate. Thereafter, the prey became immobile and rounded. Dinophysis caudata actively ingested the cytoplasm of the prey through the peduncle. Dinophysis caudata grew at a growth rate of 1.03 divisions day-1 when supplied with M. rubra as prey, reaching a maximum concentration of ca. 5,000 cell well-1 (810 μL) during a 9 day growth experiment. In contrast, a culture of D. caudata was not able to be established in the absence of the ciliate or when provided with T. amphioxeia only, suggesting that D. caudata can not directly utilize T. amphioxeia as prey. © The Plankton Society of Japan.

  41. Development of microsatellite markers in the noxious red tide-causing dinoflagellate Heterocapsa circularisquama (Dinophyceae) (Molecular Ecology Notes (2007) 7, (993-995)) 査読有り

    Nagai S, Nishitani G, Yamaguchi S, Yasuda N, Lian C.L, Itakura S, Yamaguchi M

    Molecular Ecology Resources 8 (2) 469 2008年

    DOI: 10.1111/j.1471-8286.2007.02086.x  

    ISSN:1755-098X

    eISSN:1471-8286

  42. Growth characteristics and phylogenetic analysis of the marine dinoflagellate Dinophysis infundibulus (Dinophyceae) 査読有り

    Goh Nishitani, Satoshi Nagai, Yoshihito Takano, Sanae Sakiyama, Katsuhisa Baba, Takashi Kamiyama

    AQUATIC MICROBIAL ECOLOGY 52 (3) 209-221 2008年

    出版者・発行元:INTER-RESEARCH

    DOI: 10.3354/ame01233  

    ISSN:0948-3055

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    We report the successful growth of the toxic dinoflagellate Dinophysis infundibulus under laboratory conditions, when fed the marine ciliate Myrionecta rubra grown with the cryptophyte Teleaulax amphioxeia; this study follows previous studies on D. acuminata, D. caudata, and D. fortii, showing the mixotrophy. We succeeded in maintaining several clonal strains of D. infundibulus for a relatively long period of time (> 8 mo). Growth rates in D. infundibulus ranged from 0.40 to 0.94 divisions V, reaching a maximum concentration of 1.2 to 2.3 x 103 cells ml(-1). The rate was 0.36 divisions d(-1) when apparently fully expanded cells, resulting from the active ingestion of Myrionecta rubra, were cultivated without the ciliate prey. In contrast, the culture of D. infundibulus was not established in the absence of the ciliate or when provided only with the cryptophyte T amphioxeia, suggesting that D. infundibulus cannot directly use T amphioxeia as prey. We also determined the sequences of nuclear small subunit (SSU) rRNA, 5.8S rRNA with internal transcribed spacers (ITS1 and ITS2), and large subunit (LSU) rRNA regions in D. infundibulus. Phylogeneitic relationships revealed that they are non-identical with regard to any of the DNA sequence data of Dinophysis deposited in GenBank, and are distinguishable from other Dinophysis species for species identification.

  43. Development of microsatellite markers in the noxious red tide-causing dinoflagellate Heterocapsa circularisquama (Dinophyceae) 査読有り

    S. Nagai, G. Nishitani, S. Yamaguchi, N. Yasuda, C. L. Lian, S. Itakura, M. Yamaguchi

    MOLECULAR ECOLOGY NOTES 7 (6) 993-995 2007年11月

    出版者・発行元:BLACKWELL PUBLISHING

    DOI: 10.1111/j.1471-8286.2007.01750.x  

    ISSN:1471-8278

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    We isolated 15 polymorphic microsatellites from one of the most noxious red tide-causing dinoflagellate species, Heterocapsa circularisquama. These loci provide one class of highly variable genetic markers, as the number of alleles ranged from two to six, and the estimate of gene diversity was from 0.205 to 0.684 across the 15 microsatellites. These loci have the potential to reveal genetic structure and gene flow among H. circularisquama populations.

  44. Development of microsatellite markers in the noxious red tide-causing dinoflagellate Heterocapsa circularisquama (Dinophyceae) 査読有り

    S. Nagai, G. Nishitani, S. Yamaguchi, N. Yasuda, C. L. Lian, S. Itakura, M. Yamaguchi

    MOLECULAR ECOLOGY NOTES 7 (6) 1108-1110 2007年11月

    出版者・発行元:BLACKWELL PUBLISHING

    DOI: 10.1111/j.1471-8286.2007.01750.x  

    ISSN:1471-8278

    詳細を見る 詳細を閉じる

    We isolated 15 polymorphic microsatellites from one of the most noxious red tide-causing dinoflagellate species, Heterocapsa circularisquama. These loci provide one class of highly variable genetic markers, as the number of alleles ranged from two to six, and the estimate of gene diversity from 0.205 to 0.684 across the 15 microsatellites. These loci have the potential to reveal genetic structure and gene flow among H. circularisquama populations.

  45. Development of compound microsatellite markers in the harmful red tide species Chattonella ovata (Raphidophyceae) 査読有り

    G. Nishitani, S. Nagai, C. L. Lian, H. Yamaguchi, S. Sakamoto, S. Yoshimatsu, K. Oyama, S. Itakura, M. Yamaguchi

    MOLECULAR ECOLOGY NOTES 7 (6) 1251-1253 2007年11月

    出版者・発行元:BLACKWELL PUBLISHING

    DOI: 10.1111/j.1471-8286.2007.01847.x  

    ISSN:1471-8278

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    We isolated 12 polymorphic microsatellites from the noxious red-tide-causing alga Chattonella ovata. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from four to 12, and the observed and expected heterozygosities ranged from 0.238 to 0.850 and from 0.310 to 0.889, respectively. These loci are useful for revealing the genetic structure of and gene flow among C. ovata populations.

  46. Development of compound microsatellite markers in the harmful dinoflagellate Cochlodinium polykrikoides (Dinophyceae) 査読有り

    G. Nishitani, S. Nagai, S. Sakamoto, C. L. Lian, C. K. Lee, T. Nishikawa, S. Itakura, M. Yamaguchi

    MOLECULAR ECOLOGY NOTES 7 (5) 827-829 2007年9月

    出版者・発行元:WILEY-BLACKWELL

    DOI: 10.1111/j.1471-8286.2007.01717.x  

    ISSN:1471-8278

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    We isolated 15 polymorphic microsatellites from Cochlodinium polykrikoides. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from two to 15, and the estimate of gene diversity was from 0.083 to 0.880 across the 15 microsatellites. We consider that these loci have a potential to reveal the genetic structure and gene flow among C. polykrikoides populations.

  47. Development of compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella (Dinophyceae) 査読有り

    Goh Nishitani, Satoshi Nagai, Estelle Masseret, Chunlian Lian, Sanae Yamaguchi, Nina Yasuda, Shigeru Itakura, Daniel Grzebyk, Patrick Berrebi, Masashi Sekino

    Plankton and Benthos Research 2 (3) 128-133 2007年8月

    DOI: 10.3800/pbr.2.128  

    ISSN:1880-8247

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    In this study, we identified 9 polymorphic compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella, which was isolated from Thau lagoon (France, Mediterranean Sea), using the compound microsatellite marker technique. These new microsatellites were characterized by screening DNA templates from 43 A. catenella clonal strains, which were collected from a seawater sample from Inokushi Bay (Oita Prefecture, Japan). These loci provide one class of highly variable genetic marker: the number of alleles ranged from 3 to 8, and the estimate of gene diversity varied between 0.285 and 0.762. We also analyzed the same 43 DNA samples using microsatellite markers previously identified for A. catenella, comparing the PCR amplification success, the number of alleles and gene diversity. These three parameters were not significantly different, showing that the compound microsatellite markers have the same potential to reveal A. catenella genetic structure. This simple and efficient method reduces the costs for developing SSR markers and for analyzing the genetic structure of populations, therefore, suggesting the effectiveness of applying this method to other species. © The Plankton Society of Japan.

  48. 高度多型分子マーカーを用いた有害・有毒赤潮生物の個体群構造の解析と分布拡大機構の解明

    長井 敏, 西谷 豪, 山口早苗

    海洋と生物 172 423-431 2007年

  49. Relationships between occurrences of toxic Dinophysis species (Dinophyceae) and small phytoplanktons in Japanese coastal waters 査読有り

    G Nishitani, M Yamaguchi, A Ishikawa, S Yanagiya, T Mitsuya, Imai, I

    HARMFUL ALGAE 4 (4) 755-762 2005年6月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/j.hal.2004.11.003  

    ISSN:1568-9883

    eISSN:1878-1470

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    Fluctuations of the genus Dinophysis, which contained several toxic species of diarrhetic shellfish poisoning (DSP), were investigated during blooms in Hiroshima Bay, Mutsu Bay and Ise Bay, Japan. The co-occurrences of small phytoplanktons (cryptophytes, other nanophytoplanktons, cyanobacteria and eukaryotic picophytoplanktons) were investigated to search for relationships with mixotrophic Dinophysis. Cryptophytes were divided into three size-groups based on length of their chloroplasts (> 10, 5-10 and < 5 mu m) during counting by epifluorescence microscopy. Clear relationships were not found between the occurrences of Dinophysis spp. and nanophytoplanktons, cyanobacteria and eukaryotic picophytoplanktons. However, the fluctuations of small-sized cryptophytes (< 5 mu m) showed a close relationship with that of D. acuminato in Hiroshima Bay. In Mutsu Bay, small-sized cryptophytes also accompanied the first occurrence peak of Dinophysis spp. In Ise Bay, peaks of the occurrences of middle- and small-sized cryptophytes were observed 2-3 weeks before the peak of D. acuminata. These cryptophytes decreased rapidly with increase in D. acuminata. These results suggest the possibility that smallsized cryptophytes may be food organisms for mixotrophic Dinophysis, with the abundance of Dinophysis dependent on these cryptophytes. (c) 2004 Elsevier B.V. All rights reserved.

  50. Monitoring of DSP toxins in small-sized plankton fraction of seawater collected in Mutsu Bay, Japan, by ELISA method: relation with toxin contamination of scallop 査読有り

    Imai, I, H Sugioka, G Nishitani, T Mitsuya, Y Hamano

    MARINE POLLUTION BULLETIN 47 (1-6) 114-117 2003年

    出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD

    DOI: 10.1016/S0025-326X(02)00415-0  

    ISSN:0025-326X

    eISSN:1879-3363

    詳細を見る 詳細を閉じる

    Monitorings were conducted on DSP toxins in mid-gut gland of scallop (mouse assay), cell numbers of toxic dinoflagellate species of Dinophysis, and diarrhetic shellfish poisoning (DSP) toxins in small-sized (0.7-5 mum) plankton fraction of seawater collected from surface (0 m) and 20 m depth at a station in Mutsu Bay, Aomori Prefecture, Japan, in 2000. A specific enzyme-linked immunosorbent assay (ELISA) was employed for the analysis of DSP toxins in small-sized plankton fraction using a mouse monoclonal anti-okadaic acid antibody which recognizes okadaic acid, dinophysistoxin-1, and dinophysistoxin-3. DSP toxins were detected twice in the mid-gut gland of scallops at 1.1-2.3 MU (mouse units) g(-1) on 26 June and at 0.6-1.2 MU g(-1) on 3 July, respectively. Relatively high cell densities of D. fortii were observed on 26 June and 11 September, and may only contribute to the bivalve toxicity during late June to early July. D. acuminata did not appear to be responsible for the toxicity of scallops in Mutsu Bay in 2000. ELISA monitoring of small-sized plankton fraction in seawater could detect DSP toxins two weeks before the detection of the toxin in scallops, and could do so two weeks after the loss of the bivalve toxicity by mouse assay. On 17 July, toxic D. fortii was detected at only small number, <10 cells l(-1), but DSP toxins were detected by the ELISA assay, suggesting a presence of other toxic small-sized plankton in seawater. For the purpose of reducing negative impacts of DSP occurrences, monitorings have been carried out hitherto on DSP toxins of bivalve tissues by mouse assay and on cell densities of "toxic" species of Dinophysis. Here we propose a usefulness of ELISA monitoring of plankton toxicity, especially in small-sized fraction, which are possible foods of mixotrophic Dinophysis, as a practical tool for detecting and predicting DSPs in coastal areas of fisheries grounds of bivalve aquaculture. (C) 2003 Elsevier Science Ltd. All rights reserved.

  51. Trying to cultivation of Dinophysis caudata (Dinophyceae) and the appearance of small cells. Plankton Biology and Ecology 査読有り

    Nishitani G, Miyamura K, Imai I

    Plankton Biology and Ecology 50 31-36 2003年

  52. Seasonal distribution of species of the toxic dinoflagellate genus Dinophysis in Maizuru Bay (Japan), with comments on their autofluorescence and attachment of picophytoplankton 査読有り

    Goh Nishitani, Hikaru Sugioka, Ichiro Imai

    HARMFUL ALGAE 1 (3) 253-264 2002年10月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/S1568-9883(02)00031-8  

    ISSN:1568-9883

    eISSN:1878-1470

    詳細を見る 詳細を閉じる

    The seasonal distribution of the dinoflagellate genus, Dinophysis, in Maizuru Bay, Japan, was investigated from May 1997 to December 1999. Seven species of Dinophysis were detected, including the toxic species of Dinophysis acuminata and D. forth. The most dominant species was D. acuminata, detected year-around and more abundantly during periods when water temperatures were between 15 and 18 degrees C. No relationship was found between cell abundance of Dinophysis spp. and concentrations of dissolved inorganic nutrients. Phycoerythrin containing nano- and picophytoplankton (cryptophytes and cyanobacteria), suspected to be prey of mixotrophic Dinophysis, were enumerated simultaneously. A clear relationship was not found among the cell abundances of Dinophysis spp. and nano- and picophytoplankton. Autofluorescence of Dinophysis spp. (mainly D. acuminata and D. fortii) under blue-light excitation was usually of a yellow-orange color. Occasionally, Dinophysis spp. had red autofluorescencing and yellow-orange autofluorescencing particles. The proportion of cells possessing red autofluorescence tended to be higher in the warm season. Numerous coccoid cells of picophytoplankton (ca. 1-2 mu m in diameter) were found attached to the cell surface of D. acuminata, D. fortii, etc. and food vacuole-like structures also observed. These observations suggest there is a close relationship between mixotrophic Dinophysis spp. and certain picophytoplankton. Based on our observations, the possibility that the picophytoplankton found to be attached onto Dinophysis cell surfaces are a food source for Dinophysis, and a source of DSP toxins, is discussed. (C) 2002 Elsevier Science B.V. All rights reserved.

  53. Attachment of picophytoplankton to the cell surface of the toxic dinoflagellates Dinophysis acuminata and D. fortii 査読有り

    Imai, I, G Nishitani

    PHYCOLOGIA 39 (5) 456-459 2000年9月

    出版者・発行元:INT PHYCOLOGICAL SOC

    ISSN:0031-8884

    詳細を見る 詳細を閉じる

    Coccoid picophytoplankton cells (1-2 mum in diameter) were observed to attach to the cell surface of the toxic dinoflagellates Dinophysis acuminata and D. fortii in water samples collected in Maizuru Bay, Japan. in spring of 1998. A relatively large number of picophytoplankton cells were noticed in the cingulum near the flagellar-pore region in D. fortii. These picophytoplankton cells were not cyanobacteria. Food vacuolate cells of D. fortii were observed. In view of the existence of a tongue-like structure (food ingestion organelle), the size of the aperture of the flagellar pore (cytostome) of about 1.5 mum, and the finding of picophytoplankton cells attached to the Dinophysis cell surface. we suggest that the food microorganisms for mixotrophic D. acuminata and D. fortii are picophytoplankton. The food picophytoplankton may be composed of different species. and some of them may contain diarrhetic shellfish poisoning toxins and/or their precursors. We suggest that Dinophysis species such as D. acuminata and D. fortii may originally be nontoxic and may only become toxic secondarily, through the ingestion of toxic picophytoplankton. This working hypothesis could reasonably explain the marked fluctuations observed in the toxicity of D. acuminata and D. fortii.

  54. Bacterivory of the microflagellate Dinematomonas (Dinema) sp (Euglenophyta = Euglenozoa p.p.) isolated from eutrophic sites of the Seto Inland Sea of Japan 査読有り

    O Decamp, G Nishitani, G Novarino, N Rajendran

    CRYPTOGAMIE ALGOLOGIE 21 (3) 273-282 2000年7月

    出版者・発行元:ADAC-CRYPTOGAMIE

    ISSN:0181-1568

    詳細を見る 詳細を閉じる

    The present study reports observations on the bacterivory of a large-size euglenid flagellate (body length: 30-40 Gun; average biovolume: 1.5 x 10(4) mu m(3)), tentatively identified as a species of Dinematomonas (Dinema), upon fluorescently-labelled bacteria (FLB). Flagellate and bacterial strains were isolated from eutrophic sites of the Seto Inland Sea of Japan. The size ratio between predator and prey ranged from 12:1 to 27:1. Grazing rates ranged from 0.05 to 16.9 FLB min(-1) according to experimental conditions. A significant positive linear relationship was observed between the flagellate biovolume and the grazing rate. There was no simple relationship between grazing rates and incubation temperature, and between prey concentration or viability and grazing rates. (C) 2000 Adac/Editions scientifiques et medicales Elsevier SAS.

︎全件表示 ︎最初の5件までを表示

MISC 11

  1. 麻痺性貝毒原因藻Alexandrium catenellaに寄生するAmoebophryaが宿主の個体群動態に及ぼす影響

    柴田佳紀, 山口峰生, 山本圭吾, 中嶋昌紀, 大越和加, 西谷豪

    日本ベントス学会・日本プランクトン学会合同大会講演要旨集 2021 2021年

  2. 赤潮原因種である海産渦鞭毛藻Noctiluca scintillansの日本・韓国沿岸域における遺伝的多様性

    城本将臣, 石川輝, KIM Young-Ok, 北辻さほ, 高坂祐樹, 田中雅幸, 山崎康裕, 長副聡, 山本圭吾, 大越和加, 西谷豪

    日本ベントス学会・日本プランクトン学会合同大会講演要旨集 2020 2020年

  3. 北部北太平洋に出現するカイアシ類に付着する吸管虫について

    佐藤悠真, 山口篤, DOVGAL Igor V, 遠藤宜成, 大越和加, 西谷豪

    日本ベントス学会・日本プランクトン学会合同大会講演要旨集 2013 155 2013年9月26日

  4. 津波の海に生きる未来創生 II-その後の気仙沼舞根湾調査 2013年に気仙沼舞根湾海域で発生した有害有毒プランクトン

    西谷豪, 山田雄一郎, 長坂翔子, 横山勝英, 夏池真史, 吉永郁生

    海洋と生物 35 (6) 2013年

    ISSN:0285-4376

  5. 東日本大震災による攪乱後の女川湾、佐須浜、蒲生干潟のプランクトンとベントス

    大越和加, 阿部博和, 寺本航, 大野博正, 内海修平, 鈴木陽大, 綾小路法孝, 近藤智彦, 西谷豪, 遠藤宜成

    日本ベントス学会誌 66 124-126 2012年

  6. 攪乱後の女川湾、佐須浜、蒲生干潟のプランクトン・ベントス相の現状

    大越和加, 阿部博和, 寺本航, 大野博正, 内海修平, 鈴木陽大, 綾小路法孝, 近藤智彦, 西谷豪, 遠藤宜成

    食・農・村の復興支援プロジェクト報告 1 13-15 2011年

  7. 有害渦鞭毛藻Cochlodinium polykrikoidesおよびCochlodinium sp.笠沙型のリアルタイムPCRによる種特異的検出・定量方法の確立

    坂本節子, 西谷豪, 山口峰生, 高野義人

    日本水産学会大会講演要旨集 2008 213 2008年3月27日

  8. 有毒渦鞭毛藻Dinophysis infundibulusの培養と系統解析

    西谷豪, 長井敏, 高野義人, 崎山早苗, 神山孝史

    日本水産学会大会講演要旨集 2007 144 2007年9月25日

  9. 無殻渦鞭毛藻Cochlodinium polykrikoidesのITS領域にみられる多様性について

    高野義人, 坂本節子, 西谷豪, 足立真佐雄, 山口峰生

    日本ベントス学会・日本プランクトン学会合同大会講演要旨集 2007 93 2007年9月21日

  10. 日本沿岸に出現した有害渦鞭毛藻CochlodiniumのリアルタイムPCRによる検出系の確立

    坂本節子, 高野義人, 西谷豪

    日本水産学会大会講演要旨集 2007 259 2007年3月28日

  11. 無殻渦鞭毛藻Cochlodinium polykrikoidesのITS領域にみられる反復配列について

    坂本節子, 高野義人, 西谷豪, 足立真佐雄, 山口峰生

    藻類 55 (1) 94 2007年3月10日

    ISSN:0038-1578

︎全件表示 ︎最初の5件までを表示

講演・口頭発表等 58

  1. Genetic diversity of the red tide-forming dinoflagellate, Noctiluca scintillans, in Japanese and Korean coastal waters

    Shiromoto, M., Ishikawa, A., Kim, Y., Kitatsuji, S., Kosaka, Y., Tanaka, M., Yamasaki, Y., Sato-Okoshi, W., Nishitani, G.

    4th Asian Marine Biology Symposium 2019年11月5日

  2. Seasonal change of benthic diatoms in Gamo Lagoon, Miyagi Prefecture, northeastern Japan

    Kato, M. Kondoh, T., Ito, K., Nishitani, G., Sato-Okoshi, W.

    4th Asian Marine Biology Symposium 2019年11月5日

  3. Population dynamics of Boccardia proboscidea and Boccardiella hamata (Annelida, Spionidae) inhabiting oyster beds in Sasuhama, northeastern Japan.

    Manzhi, T., Okoshi, K., Nishitani, G. Sato-Okoshi, W.

    4th Asian Marine Biology Symposium 2019年11月5日

  4. 日本および韓国沿岸域から単離した夜光虫の遺伝的多様性

    西谷豪・城本将臣・Young-OK Kim・北辻さほ・田中雅幸・山﨑康裕・大越和加・石川輝

    2019年 日本ベントス学会・日本プランクトン学会合同大会 2019年9月20日

  5. 東北地方太平洋沖地震前後における女川湾湾奥のマクロベントス群集構造の変化 ( 2007 – 2019 )

    長崎礼資・片桐耕・岩渕巽・阿部博和・小林元樹・西谷豪・大越和加

    2019年 日本ベントス学会・日本プランクトン学会合同大会 2019年9月20日

  6. Development of new primer sets to detect prey DNA from marine dinoflagellate, Dinophysis spp. 国際会議

    Goh Nishitani, Natsumi Takagi, Yoshihito Takano, Satoshi Nagai, Young-Ok Kim, Yuki Kosaka, Akira Ishikawa

    10th EASTHAB Symposium 2017年12月13日

  7. Temporal variation of benthic community assemblage in Gamo Lagoon (Northeast Japan) for five years after the Great East Japan Earthquake and tsunami 国際会議

    Tomohiko Kondoh, Waka Sato-Okoshi, Goh Nishitani, Yoshinari Endo

    The Japanese Society of Fisheries Science International Symposium 2017年9月22日

  8. A molecular tool for detecting prey items of a heterotrophic marine dinoflagellate, Dinophysis rotundata 国際会議

    Goh Nishitani, Natsumi Takagi, Yoshihito Takano, Satoshi Nagai, Young-Ok Kim, Akira Ishikawa

    The Japanese Society of Fisheries Science International Symposium 2017年9月22日

  9. 地震・津波による撹乱前後の女川湾のマクロベントス群集動態と環境要因との関連

    岩渕巽, 阿部博和, 小林元樹, 西谷豪, 遠藤宜成, 大越和加

    2017年 日本プランクトン学会・日本ベントス学会合同大会 2017年9月3日

  10. 宮城県蒲生干潟における Monocorophium insidiosumの形態学および生態学的特徴

    近藤智彦, 大越和加, 西谷豪, 遠藤宜成

    2017年 日本プランクトン学会・日本ベントス学会合同大会 2017年9月3日

  11. Octopus Life-Support: Investigations of the Early Life and Behaviour of Octopus sinensis to Surmount the Paralarval Barriers to Successful Aquaculture 国際会議

    Ian G. Gleadall, Leo J.-H. Che, Kinuko Ito, Goh Nishitani, Delta Putra

    22nd International Congress of Zoology 2016年11月14日

  12. 女川湾における一次生産の季節変動

    宍戸雄太, 遠藤宜成, 大越和加, 西谷豪

    日本海洋学会 2016年度春季大会 2016年3月15日

  13. Recent progress in culturing the East Asian common octopus 国際会議

    Ian G. Gleadall, Goh Nishitani, Masami Abe, Keiji Matsubara, Shunzo Morii, Nobuhiko Akiyama, Leo J. Che, Masazumi Nishikawa

    The Cephalopod International Advisory Council 2015 2015年11月6日

  14. Is the life cycle of Ephelota sp. adapted to the molt cycle of the euphausiid Euphausia pacifica? 国際会議

    Endo,Y, Fujii,D, Nishitani, G, Wiebe, P.H

    The 4th workshop sponsored by the International Research Coordination Network for Biodiversity of Ciliates (IRCN-BC). 2015年10月19日

  15. 京都府産養殖トリガイ(Fulvia mutica)の胃腸管内から検出した遺伝子配列

    西谷 豪, 久田哲二, 長副 聡

    平成27年度日本水産学会秋季大会 2015年9月22日

  16. 宮城県気仙沼湾における麻痺性貝毒原因渦鞭毛藻Alexandrium catenella/tamarenseシストの発芽フラックス定量の試み

    夏池真史, 工大院, 理工, 横山勝英, 首都, 都市環境, 西谷 豪(東北, 山田雄一郎, 北里, 海洋生命, 吉永郁生, 石川 輝, 三重, 物資源

    2015年 日本ベントス学会・日本プランクトン学会合同大会 2015年9月2日

  17. 東北地方太平洋沖地震と津波による攪乱後の蒲生干潟における底生生物群集の変遷(2011年~2015年)

    近藤智彦, 中山凱, 西谷豪, 遠藤宜成, 大越和加

    2015年 日本ベントス学会・日本プランクトン学会合同大会 2015年9月2日

  18. 女川湾におけるカイアシ類群集の季節変動と生産量について

    佐藤浩樹, 西谷豪, 大越和加, 遠藤宜成

    2015年 日本ベントス学会・日本プランクトン学会合同大会 2015年9月2日

  19. 地震と津波による攪乱が蒲生干潟の底生生物群集に及ぼす継続的影響

    近藤智彦, 中山凱, 西谷豪, 遠藤宜成, 大越和加

    平成27年マリンサイエンス拠点形成事業全体会議 2015年6月6日

  20. 天然タコ幼生の胃内容物から検出した遺伝子配列

    西谷 豪, Ian, G. Gleadall, 上原一晃, 松原圭史, 阿部正美, 秋山信彦, 西川正純

    平成26年度日本水産学会東北支部大会 2014年11月

  21. Relationship between occurrence of the toxic dinoflagellate Dinophysis acuminata and ciliate Mesodinium rubrum in the coastal water of Japan 国際会議

    Akira Ishikawa, Goh Nishitani, Young-Ok Kim, Yoshihito Takano, Satoshi Nagai

    International Conference on Harmful Algae 2014 2014年10月27日

  22. 東北地方太平洋沖地震後の七田北川河口におけるマクロベントスの群集構造

    中山凱, 近藤智彦, 西谷豪, 遠藤宜成, 大越和加

    2014年度日本プランクトン学会・日本ベントス学会合同大会 2014年9月

  23. 東北地方太平洋沖地震後の宮城県佐須浜マガキ帯に生息するスピオ科多毛類2種の個体群動態

    田中秀樹, 遠藤宜成, 大越和加, 西谷豪

    2014年度日本プランクトン・ベントス学会 2014年9月

  24. 東北地方太平洋沖地震と津波による攪乱後のスピオ科多毛類Pseudopolydora cf. kempiとP. reticulataの個体群動態

    近藤智彦, 阿部博和, 金谷弦, 西谷豪, 遠藤宜成, 大越和加

    2014年度日本プランクトン学会・ベントス学会合同大会 2014年9月

  25. 東北地方太平洋沖地震と津波による攪乱後の蒲生干潟(宮城県)における二枚貝類群集

    近藤智彦, 西谷豪, 遠藤宜成, 大越和加

    平成26年度日本水産学会秋季大会 2014年9月

  26. Planktonic octopus paralarvae in the East China Sea

    Ian G. Gleadall, Goh Nishitani, Kazuaki Uehara, Hiroto Teranaka, Masumi Abe, Shunzo Mori, Kenji Matsubara, Nobuhiko Akiyama, Masazumi Nishikawa

    日本動物学会第85回仙台大会 2014年9月

  27. 日本産カキ類4種における血リンパレクチンの赤血球凝集活性

    伊藤直樹, 高橋計介, 中根基行, 西谷豪

    平成26年度日本水産学会秋季大会 2014年9月

  28. A joint academic- industrial project to culture the Asian common octopus commercially using land-based aquaria. 国際会議

    Gleadall, I.G, G. Nishitani, M. Abe, S. Morii, K. Matsubara, N. Akiyama, M. Nishikawa

    ICES Annual Science Conference 2014年9月

  29. 有害有毒プランクトンの発生状況-気仙沼舞根湾調査-

    西谷豪, 山田雄一郎, 長坂翔子, 横山勝英, 夏池真史, 吉永郁生

    平成26年度日本水産学会春季大会水産環境保全委員会シンポジウム 2014年3月

  30. 女川湾における水産有用貝類の貝殻に穿孔するスピオ科多毛類Polydora onagawaensis の分布、侵蝕状況、生活史

    寺本航, 西谷豪, 遠藤宜成, 大越和加

    平成26年度日本水産学会春季大会 2014年3月

  31. マガキを中心とした二枚貝浮遊幼生の分類とその応用について

    西谷 豪, 渡辺允浩, 渡辺 茂

    平成25年度日本水産学会東北支部大会 2013年11月8日

  32. 2013年春季の宮城県気仙沼湾における有毒渦鞭毛藻Alexandrium tamarenseの大発生

    山田雄一郎, 西谷豪, 畠山信, 横山勝英, 吉永郁生, 田中克

    平成25年度日本水産学会東北支部大会 2013年11月8日

  33. 2013年春季の宮城県気仙沼湾における有毒渦鞭毛藻Alexandrium tamarenseの大発生

    山田雄一郎, 西谷豪, 畠山信, 横山勝英, 吉永郁生, 田中克

    2013年 日本ベントス学会・日本プランクトン学会合同大会 2013年9月27日

  34. 宮城県松島湾における二枚貝浮遊幼生の分類と動態に関する研究

    西谷 豪, 渡辺允浩, 渡辺 茂

    2013年 日本ベントス学会・日本プランクトン学会合同大会 2013年9月27日

  35. 自然水域における有毒渦鞭毛藻Dinophysis acuminata と繊毛虫Mesodinium rubrum の出現関係

    石川輝, 真野優子, 西谷豪, 金英玉, 長井敏

    2013年 日本ベントス学会・日本プランクトン学会合同大会 2013年9月27日

  36. 多毛類スピオ科Pseudopolydora cf. kempiとP. reticulata の発生様式と分布

    近藤智彦, 阿部博和, 西谷豪, 遠藤宜成, 大越和加

    2013年 日本ベントス学会・日本プランクトン学会合同大会 2013年9月27日

  37. 東北地方太平洋沖地震前後の女川湾における多毛類の群集構造

    小林元樹, 大越和加, 西谷豪, 遠藤宜成

    2013年 日本ベントス学会・日本プランクトン学会合同大会 2013年9月27日

  38. スピオ科Pseudopolydora属多毛類の日本初記録種2種について

    阿部博和, 近藤智彦, 西谷豪, 遠藤宜成, 大越和加

    2013年 日本ベントス学会・日本プランクトン学会合同大会 2013年9月27日

  39. 付着性繊毛虫のツノナシオキアミに対する付着率の季節変化

    藤井大樹, 大越和加, 西谷豪, 遠藤宜成

    2013年 日本ベントス学会・日本プランクトン学会合同大会 2013年9月27日

  40. 北部北太平洋に出現するカイアシ類に付着する吸管虫について

    佐藤悠真, 山口篤・Igor V. Dovgal, 遠藤宜成, 大越和加, 西谷豪

    2013年 日本ベントス学会・日本プランクトン学会合同大会 2013年9月27日

  41. 女川湾の微生物環構成生物は震災前後でどう変化したか

    紙谷紹夫, 大越和加, 西谷豪, 遠藤宜成

    2013年度 日本海洋学会秋季大会 2013年9月17日

  42. 東日本大震災後の宮城県女川湾における珪藻群集動態

    黒柳有紀, 遠藤宜成, 大越和加, 西谷豪

    2013年度 日本海洋学会秋季大会 2013年9月17日

  43. Reproduction and larval development of Pseudopolydora cf. kempi and P. reticulata (Polychaeta, Spionidae) in Gamo Lagoon, northeastern Japan. 国際会議

    Kondoh T, Abe H, Sato-Okoshi W, Nishitani G, Endo Y

    The 11th International Polychaete Conference 2013年8月4日

  44. A new species of Polydora (Polychaeta: Spionidae) from northeastern Japan. 国際会議

    Teramoto W, Sato-Okoshi W, Nishitani G, Endo Y

    The 11th International Polychaete Conference 2013年8月4日

  45. Life history of Polydora onagawaensis (Polychaeta: Spionidae) from Northeastern Japan. 国際会議

    Teramoto W, Sato-Okoshi W, Nishitani G, Endo Y

    The 11th International Polychaete Conference 2013年8月4日

  46. Vertical distribution and migration of planktonic polychaete larvae in Onagawa Bay, northeastern Japan.

    Hirokazu Abe, Waka Sato-Okoshi, Goh Nishitani, Yoshinari Endo

    The 11th International Polychaete Conference 2013年8月4日

  47. Molecular analysis of the 18S rRNA gene of spionid polychaets. 国際会議

    Hirokazu Abe, Waka Sato-Okoshi, Goh Nishitani, Yoshinari Endo

    The 11th International Polychaete Conference 2013年8月4日

  48. "有害有毒プランクトンの発生状況-気仙沼舞根湾調査-

    西谷 豪, 山田雄一郎, 長坂翔子, 横山勝英, 夏池真史, 吉永郁生

    平成26年度日本水産学会春季大会シンポジウム 2013年3月27日

  49. 女川湾における水産有用貝類の貝殻に穿孔するスピオ科多毛類Polydora onagawaensis の分布、侵蝕状況、生活史.

    寺本航, 西谷豪, 遠藤宜成, 大越和加

    平成26年度日本水産学会春季大会 2013年3月27日

  50. オキアミ類に付着する繊毛虫の分類学的研究

    遠藤宜成, 西谷 豪, 小林俊将, 中野伸行

    2012年度日本海洋学会春季大会 2012年3月

  51. 女川湾に出現する多毛類浮遊幼生の同定と季節変動

    阿部博和, 西谷 豪, 遠藤宜成, 大越和加

    平成24年度日本水産学会春季大会 2012年3月

  52. 気仙沼・舞根湾における生物環境調査-3 プランクトンの現況

    山田雄一郎, 西谷 豪

    平成24年度日本水産学会春季大会 2012年3月

  53. 海洋プランクトン

    西谷 豪, 山田雄一郎

    森は海の恋人シンポジウム「海と共に生きる」 2011年12月

  54. Morphology, feeding characteristics and taxonomy of a novel diatom-feeding flagellate isolated from bottom sediment of Onagawa Bay, northeastern Japan. 国際会議

    Hiromasa Ohno, Goh Nishitani, Waka Sato-Okoshi, Yoshinari Endo

    1st Asian Congress of Protistology 8th Asian Conference on Ciliate Biology 2011年10月

  55. 形態及び分子生物学的手法を用いた多毛類浮遊幼生の同定の試み

    阿部博和, 西谷 豪, 遠藤宜成, 大越和加

    2011年度日本ベントス学会・日本プランクトン学会合同大会 2011年9月

  56. 海産繊毛虫Spirotontonia grandisから検出された葉緑体配列の遺伝的多様性

    西谷豪, 鈴木利一

    日本藻類学会第35回大会 2011年3月26日

  57. 有毒渦鞭毛藻Dinophysis triposの増殖特性と毒生産

    神山孝史, 長井 敏, 西谷 豪, 鈴木敏之

    平成22年度日本水産学会秋季大会 2010年9月

  58. 海産浮遊性プランクトンの次世代シーケンス網羅解析による生物多様性比較

    長井 敏, 西谷 豪, 野口大毅, 阿部和雄

    日本進化学会シンポジウム 2010年8月

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共同研究・競争的資金等の研究課題 7

  1. 細胞内遺伝子解析が明らかにする海産有毒微細藻ディノフィシス属の餌料生物

    2019年4月 ~ 2020年3月

  2. 混合栄養性有毒渦鞭毛藻と餌生物との動的関係

    石川 輝, 西谷 豪

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Scientific Research (C)

    研究機関:Mie University

    2013年4月1日 ~ 2016年3月31日

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    伊勢湾内に設けた調査点から混合栄養性有毒渦鞭毛藻Dinophysis acuminataを採取して,その細胞の食胞内に残存する遺伝子を解析し餌生物の特定を試みた。その結果,いずれのD. acuminata細胞からも繊毛虫Mesodinium rubrumが検出された。さらに,調査点においてD. acuminataとM. rubrumの出現関係を季節的に調べたところ, D. acuminataの増減は,やはり餌生物であるM. rubrumの出現により左右されている場合があることを示し得た。なお,本研究ではD. rotundataについても,D. acuminataと同様の研究を行った。

  3. 震災後の宮城県松島湾におけるマガキ養殖場の餌料環境調査と再生への取り組み

    西谷 豪

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Young Scientists (B)

    研究機関:Tohoku University

    2012年4月1日 ~ 2015年3月31日

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    宮城県松島湾から合計516個体の二枚貝浮遊幼生を単離し、遺伝子同定によって合計33種の二枚貝浮遊幼生を検出した。その中で特に出現率の高かった7種を識別するマルチプレックスPCR法を確立したことによって、PCRと電気泳動のみで松島湾に出現する浮遊幼生を種ごとに識別して動態を把握することが可能となった。現場に生息するマガキ親個体の餌料生物を解析した結果、サイズは15-335μmの範囲で粒子が観察され、特に20-70μmの粒子が80-90%を占めていた。珪藻、渦鞭毛藻、ボリド藻、従属栄養性鞭毛虫、繊毛虫、ホヤ、紅藻、カイアシ類の遺伝子が検出された。

  4. 有毒渦鞭毛藻ディノフィシスが捕食する繊毛虫およびその餌料生物の多様性と分子系統

    西谷 豪

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Young Scientists (B)

    2009年 ~ 2011年

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    本研究では、養殖二枚貝類に下痢性貝毒を引き起こす原因生物である海洋性植物プランクトンのディノフィシス属を主な研究対象とした。成果として、主要なディノフィシス属が現場海域で捕食する繊毛虫はミリオネクタと呼ばれるものであり、そのミリオネクタはある特定のクリプト藻種を選択的に捕食していることが判明した。また、ディノフィシスの中には100種類以上を超える葉緑体を細胞内に保持している種も存在し、ディノフィシスが複雑な海洋生態系の中で果たしている役割の一端を明らかにした。

  5. 下痢性貝毒原因種Dinophysis属の増殖生理と毒生産能に関する研究

    神山 孝史, 鈴木 敏之, 長井 敏, 西谷 豪

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:Fisheries Research Agency

    2008年 ~ 2011年

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    Dinophysis属の栄養生理特性、増殖・毒生産能とそれらに及ぼす環境条件の影響を調べた。国内各地における自然群集のDinophysis属各種の細胞毒量の特徴を明らかにした。本属の増殖は、クリプト藻Teleaulax amphioxeiaを餌とする繊毛虫Myrionecta rubraの存在に強く依存し、温度、光の影響を強く受け、一部の毒成分は増殖の進行と共に細胞外に活発に排出された。同じ環境下で培養された同一種の毒量や毒組成が株間で大きく違う場合があり、遺伝的要因の関与が示唆された。

  6. Study on the mixotrophic and toxic dinoflagellate genus Dinophysis (Dinophyceae) : Population dynamics in coastal waters and trial of establishment of culture in laboratory 競争的資金

  7. 沿岸域における混合栄養性有毒渦鞭毛藻Dinophysis属の動態および培養の試みに関する研究 競争的資金

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メディア報道 6

  1. 読売新聞 執筆者本人

    2022年4月

    メディア報道種別: 新聞・雑誌

  2. 東海新報 執筆者本人

    2022年4月

    メディア報道種別: 新聞・雑誌

  3. NHK全国放送 執筆者本人

    2022年2月

  4. NHK仙台放送「てれまさむね」 執筆者本人

    2021年12月

    メディア報道種別: テレビ・ラジオ番組

  5. 東日本放送「チャージ」 執筆者本人

    2021年12月

    メディア報道種別: テレビ・ラジオ番組

  6. こどものじかん

    仙台放送

    2018年1月28日

    メディア報道種別: テレビ・ラジオ番組

    詳細を見る 詳細を閉じる

    番組内で研究が紹介された

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