顔写真

キクサト モトイ
喜久里 基
Motoi Kikusato
所属
大学院農学研究科 生物生産科学専攻 動物生命科学講座(動物栄養生化学分野)
職名
准教授
学位
  • 博士(農学)(東北大学)

  • 修士(農学)(東北大学)

e-Rad 研究者番号
90613042

経歴 1

  • 2008年4月 ~ 2011年3月
    東北大学大学院農学研究科 日本学術振興会DC1特別研究員

学歴 2

  • 東北大学 農学研究科 応用生命科学

    ~ 2011年3月25日

  • 鹿児島大学 農学部 生物資源化学科

    ~ 2006年3月25日

委員歴 6

  • 日本家禽学会 機関紙編集委員(The Journal of Poultry Science, Section Editor)

    2022年4月 ~ 継続中

  • Frontiers in Physiology Associate Editor

    2021年10月 ~ 継続中

  • 日本家禽学会 国際交流委員

    2021年4月 ~ 継続中

  • 日本家禽学会 評議員

    2021年4月 ~ 継続中

  • 日本畜産学会 機関紙編集委員(Associate Editor)

    2020年4月 ~ 継続中

  • Journal of Feed and Animal Sciences Editorial Board Member (Associate Editor)

    2019年4月 ~ 継続中

︎全件表示 ︎最初の5件までを表示

所属学協会 5

  • 世界家禽学会

  • 家畜栄養生理研究会

  • 東北畜産学会

  • 日本畜産学会

  • 日本家禽学会

研究キーワード 7

  • ニワトリ

  • ポリフェノール

  • 飼料添加物

  • 暑熱ストレス

  • 活性酸素

  • エネルギー代謝

  • ミトコンドリア

研究分野 1

  • ライフサイエンス / 動物生産科学 /

受賞 4

  1. 日本畜産学会 Animal Science Journal Best Reviewers Awards

    2020年3月 日本畜産学会

  2. Animal Science Journal Best Reviewers Awards

    2017年3月 日本畜産学会

  3. Young Investigator Awards, 5th SFRR-Asia/8th ASMRM/11th J-mit International Conference

    2011年9月4日 5th SFRR-Asia/8th ASMRM/11th J-mit International Conference

  4. 日本家禽学会2010年度春季学術大会 優秀発表賞

    2010年3月30日 日本家禽学会 急性暑熱感作における活性酸素種(ROS)の産生増大はどんなときにおこるか?

論文 52

  1. Possible role of corticosterone in proteolysis, glycolytic, and amino acid metabolism in primary cultured avian myotubes incubated at high-temperature conditions 査読有り

    Kyohei Furukawa, Masaaki Toyomizu, Motoi Kikusato

    Domestic Animal Endocrinology 76 106608-106608 2021年7月

    出版者・発行元:Elsevier BV

    DOI: 10.1016/j.domaniend.2021.106608  

    ISSN:0739-7240

  2. Phytobiotics to improve health and production of broiler chickens: functions beyond the antioxidant activity 査読有り

    Motoi Kikusato

    Animal Bioscience 34 (3) 345-353 2021年3月1日

    出版者・発行元:Asian Australasian Association of Animal Production Societies

    DOI: 10.5713/ab.20.0842  

    ISSN:2765-0189

    eISSN:2765-0235

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    Phytobiotics, also known as phytochemicals or phytogenics, have a wide variety of biological activities and have recently emerged as alternatives to synthetic antibiotic growth promoters. Numerous studies have reported the growth-promoting effects of phytobiotics in chickens, but their precise mechanism of action is yet to be elucidated. Phytobiotics are traditionally known for their antioxidant activity. However, extensive investigations have shown that these compounds also have anti-inflammatory, antimicrobial, and transcription-modulating effects. Phytobiotics are non-nutritive constituents, and their bioavailability is low. Nonetheless, their beneficial effects have been observed in several tissues or organs. The health benefits of the ingestion of phytobiotics are attributed to their antioxidant activity. However, several studies have revealed that not all these benefits could be explained by the antioxidant effects alone. In this review, I focused on the bioavailability of phytobiotics and the possible mechanisms underlying their overall effects on intestinal barrier functions, inflammatory status, gut microbiota, systemic inflammation, and metabolism, rather than the specific effects of each compound. I also discuss the possible mechanisms by which phytobiotics contribute to growth promotion in chickens.

  3. Effects of plant-derived isoquinoline alkaloids on growth performance and intestinal function of broiler chickens under heat stress 査読有り

    Motoi Kikusato, Guangda Xue, Anja Pastor, Theo A. Niewold, Masaaki Toyomizu

    Poultry Science 100 (2) 957-963 2021年2月

    出版者・発行元:Elsevier BV

    DOI: 10.1016/j.psj.2020.11.050  

    ISSN:0032-5791

  4. Effect of dipeptide on intestinal peptide transporter 1 gene expression: An evaluation using primary cultured chicken intestinal epithelial cells 査読有り

    Yukako Tokutake, Marcin Taciak, Kan Sato, Masaaki Toyomizu, Motoi Kikusato

    Animal Science Journal 92 (1) 2021年1月

    出版者・発行元:Wiley

    DOI: 10.1111/asj.13604  

    ISSN:1344-3941

    eISSN:1740-0929

  5. Effect of trehalose supplementation on growth performance and intestinal morphology in broiler chickens 査読有り

    Yuwares Ruangpanit, Koichi Matsushita, Kazuhisa Mukai, Motoi Kikusato

    Veterinary and Animal Science 10 100142-100142 2020年12月

    出版者・発行元:Elsevier BV

    DOI: 10.1016/j.vas.2020.100142  

    ISSN:2451-943X

  6. Differences in breast muscle mitochondrial respiratory capacity, reactive oxygen species generation, and complex characteristics between 7-week-old meat- and laying-type chickens. 査読有り

    Yuki Hakamata, Masaaki Toyomizu, Motoi Kikusato

    The Journal of Poultry Science 57 (4) 319-327 2020年10月25日

    DOI: 10.2141/jpsa.0190133  

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    The skeletal muscle growth rate is a major feature differentiating meat- and laying-type chickens. A large amount of ATP is required during skeletal muscle synthesis, in which mitochondrial energy production capacities play a significant role. Additionally, mitochondria may participate in muscle protein degradation via reactive oxygen species generation. To investigate the differences in mitochondrial energetic characteristics between chickens exhibiting different growth rates, this study evaluated respiratory capacities in response to different types of respiratory substrate, protein abundances, assembly of individual respiratory complexes (I-V) and supercomplexes, and reactive oxygen species generation rates. These characteristics were compared between mitochondria from the breast muscle (M. pectoralis superficialis) of seven-week-old meat- and laying-type male chickens. Blue native polyacrylamide gel electrophoresis analysis revealed that meat-type chickens exhibited a significantly lower protein abundance of complex III (cytochrome bc1 complex), complex V (F0F1 ATP synthase), and total amount of supercomplexes than did laying-type chickens. There were no differences between chicken types in the respiration rate of mitochondria incubated with either pyruvate/malate or succinate, each of which drives complex I- and complex II-linked respiration. Carnitine palmitoyltransferase-1-dependent and -independent respiration during ATP synthesis and carnitine palmitoyltransferase-2 enzymatic activity were significantly lower in meat-type chickens than in layingtype chickens. For mitochondria receiving pyruvate/malate plus succinate, the reactive oxygen species generation rate and its ratio to the oxygen consumed (the percentage of free radical leak) were also significantly lower in meat-type chickens than in laying-type chickens. These results suggested that the mitochondrial energetic capacities of the breast muscle of meat-type chickens could be lower than those of laying-type chickens at seven weeks of age. Furthermore, the lower reactive oxygen species generation rate in meat-type chickens might have implications for rapid muscle development, which is possibly related to their lower muscle protein degradation rates.

  7. Effects of low-dose oleuropein diet supplementation on the oxidative status of skeletal muscles and plasma hormonal concentration of growing broiler chickens 査読有り

    R. Shimao, H. Muroi, K. Furukawa, M. Toyomizu, M. Kikusato

    British Poultry Science 60 (6) 784-789 2019年11月2日

    DOI: 10.1080/00071668.2019.1662886  

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    © 2019, © 2019 British Poultry Science Ltd. 1. Oleuropein (Ole) is a major phenolic compound in Olea europaea, with anti-oxidative, anti-obesity, and anti-inflammatory properties. To explore the effect of Ole on the physiology and metabolism of poultry, this study, evaluated the effects of feeding low-dose Ole on the growth performance, metabolic hormonal status, muscle oxidative status in growing broiler chickens. 2. Thirty-two 8-day-old chickens were assigned to four different treatments, and fed either 0 (control), 0.1, 0.5, or 2.5 ppm Ole-supplemented diets for 2 weeks. 3. There were no differences in the body weight gain, feed consumption, and feed efficiency during the feeding periods between the groups tested. Birds fed Ole 0.5- and 2.5 ppm-supplemented diets exhibited a significant decrease in muscle carbonyl content compared to the control group. In the group fed Ole 0.5 ppm, the mRNA expression levels of mitochondrial ROS-reducing factors: avian uncoupling protein and manganese superoxide dismutase, as well as peroxisome proliferator-activated receptor γ coactivator 1-α, sirtuin-1 and −3 (each of which co-ordinately induce the transcription of the previous two factors) were upregulated compared to the control group, and the changes were independent of plasma noradrenaline and thyroid hormone levels. The group fed Ole-2.5 ppm did not show such transcriptional changes, but exhibited a higher corticosterone concentration. 4. This study demonstrates that ingesting a low dose of Ole can reduce muscle oxidative damage, and that the suppression machinery may differ depending on the amount of Ole ingested by growing broiler chickens.

  8. Differential effects of heat stress on oxidative status of skeletal muscle with different muscle fibre compositions in broiler chicken 査読有り

    M. Kikusato, M. Toyomizu

    Journal of Animal and Feed Sciences 28 (1) 78-82 2019年1月31日

    出版者・発行元:The Kielanowski Institute of Animal Physiology and Nutrition, {PAS}

    DOI: 10.22358/jafs/102830/2019  

  9. Characterization of mitochondrial content and respiratory capacities of broiler chicken skeletal muscles with different muscle fiber compositions. 査読有り

    Yuki Hakamata, Kouichi Watanabe, Taku Amo, Masaaki Toyomizu, Motoi Kikusato

    The Journal of Poultry Science 55 (3) 210-216 2018年

    DOI: 10.2141/jpsa.0170141  

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    Mitochondrial content is regarded a useful feature to distinguish muscle-fiber types in terms of energy metabolism in skeletal muscles. Increasing evidence suggests that specific mitochondrial bioenergetic phenotypes exist in metabolically different muscle fibers. A few studies have examined the energetic properties of skeletal muscle in domestic fowls; however, no information on muscle bioenergetics in broiler chickens selectively bred for faster growth is available. In this study, we aimed to characterize the mitochondrial contents and functions of chicken skeletal muscle consisting entirely of type I (oxidative) (M. pubo-ischio-femoralis pars medialis), type IIA (glycolytic/oxidative) (M. pubo-ischio-femoralis pars lateralis), and type IIB (glycolytic) (M. pectoralis) muscle fibers. Citrate synthase (CS) activity was the highest in type IIA muscle tissues and isolated mitochondria, among the muscle tissues tested. Although no difference was registered in mitochondrial CS activity between type IIB and type I muscles, tissue CS activity was significantly higher in the latter. Histochemical staining for NADH tetrazolium reductase and the ratio of muscle-tissue to mitochondrial CS activity indicated that type I, type IIA, and type IIB muscle-fiber types showed decreasing mitochondrial content. Mitochondria from type I muscle exhibited a higher coupled respiration rate induced by pyruvate/malate, palmitoyl-CoA/malate, and palmitoyl-carnitine, as respiratory substrates, than type IIB-muscle mitochondria, while the response of mitochondria from type IIA muscle to those substrates was comparable to that of mitochondria from type I muscle. Type IIA-muscle mitochondria exhibited the highest carnitine palmitoyltransferase-2 level among all tissues tested, which may contribute to the higher fatty acid oxidation in these mitochondria. The results suggest that mitochondrial abundance is one of the features differentiating metabolic characteristics of different chicken skeletal muscle types. Moreover, the study demonstrated that type IIA-muscle mitochondria may have distinct metabolic capacities.

  10. Oleuropein induces mitochondrial biogenesis and decreases reactive oxygen species generation in cultured avian muscle cells, possibly via an up-regulation of peroxisome proliferator-activated receptor γ coactivator-1α 国際誌 査読有り

    Kikusato, M, Muroi, H, Uwabe, Y, Furukawa, K, Toyomizu, M

    Animal Science Journal 87 (11) 1371-1378 2016年11月

    出版者・発行元:None

    DOI: 10.1111/asj.12559  

    ISSN:1344-3941

    eISSN:1740-0929

  11. Effects of trehalose supplementation on the growth performance and intestinal innate immunity of juvenile chicks 査読有り

    M. Kikusato, F. Nanto, K. Mukai, M. Toyomizu

    British Poultry Science 57 (3) 375-380 2016年6月

    出版者・発行元:TAYLOR & FRANCIS LTD

    DOI: 10.1080/00071668.2016.1166475  

    ISSN:0007-1668

    eISSN:1466-1799

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    Trehalose is composed of two molecules of D-glucose joined by an ,-1,1 glucosidic linkage and has antioxidative and anti-inflammatory effects. The present study investigated the effect of feeding a trehalose-supplemented diet on the growth performance, as well as the oxidative status and the intestinal innate immunity of juvenile chicks.A total of 16 d-old male broiler chicks were used in this study: two groups of 8 birds were fed on a 0% (control) or 0.5% trehalose-supplemented diet for 18 d.The mean body weight of the trehalose group was significantly greater than that of the control group, but feed efficiency was not altered by feeding the trehalose-supplemented diet. No differences in the levels of lipid peroxidation in skeletal muscle, liver and plasma were observed between the control and trehalose-supplemented groups. The mRNA levels of interferon-, tumour necrosis factor-like ligand 1A, interleukin-10, NADPH oxidase 4 and inducible NO synthase were significantly reduced by the trehalose supplementation.Our results suggest that dietary supplementation with trehalose after hatching may have beneficial effects on the growth performance of juvenile chicks, probably by improving their intestinal innate immunity.

  12. Effect of Dietary 4-Phenylbuthyric Acid Supplementation on Acute Heat-Stress-Induced Hyperthermia in Broiler Chickens. 国際誌

    Yukako Tokutake, Ryo Takanashi, Motoi Kikusato, Masaaki Toyomizu, Kan Sato

    Animals : an open access journal from MDPI 12 (16) 2022年8月12日

    DOI: 10.3390/ani12162056  

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    Hot, humid weather causes heat stress (HS) in broiler chickens, which can lead to high mortality. A recent study found that HS causes endoplasmic reticulum (ER) stress. However, the possible involvement of ER stress in HS-induced physiological alterations in broiler chickens is unclear. This study aimed to evaluate the effect of the dietary supplementation of 4-phenylbutyric acid (4-PBA), an alleviator of ER stress, in acute HS-exposed young broiler chickens. Twenty-eight 14-day-old male broiler chickens (ROSS 308) were divided into two groups and fed either a control diet or a diet containing 4-PBA (5.25 g per kg of diet feed) for 10 days. At 24 days old, each group of chickens was kept in thermoneutral (24 ± 0.5 °C) or acute HS (36 ± 0.5 °C) conditions for 2 h. The results showed that thermoneutral birds supplemented with 4-PBA exhibited no negative effects in terms of broiler body weight gain and tissue weight compared to non-supplemental birds. HS increased body temperature in both the control and 4-PBA groups, but the elevation was significantly lower in the 4-PBA group than in the control group. The plasma non-esterified fatty acid concentration was significantly increased by HS treatment in non-supplemental groups, while the increase was partially attenuated in the 4-PBA group. Moreover, 4-PBA prevented HS-induced gene elevation of the ER stress markers GRP78 and GRP94 in the skeletal muscle. These findings suggest that the 4-PBA effect may be specific to the skeletal muscle in HS-exposed birds and that 4-PBA supplementation attenuated HS-induced muscle ER stress, which could be associated with a supplementation of the body temperature elevation and lipolysis.

  13. Characteristics of Essential Oils of <i>Apiaceae </i>Family: Their Chemical Compositions, <i>in vitro </i>Properties and Effects on Broiler Production 招待有り 査読有り

    Usman Ali, Saima Naveed, Shafqat Nawaz Qaisrani, Athar Mahmud, Zafar Hayat, Muhammad Abdullah, Motoi Kikusato, Masaaki Toyomizu

    The Journal of Poultry Science 59 (1) 16-37 2022年

    出版者・発行元:Japan Poultry Science Association

    DOI: 10.2141/jpsa.0210042  

    ISSN:1346-7395

    eISSN:1349-0486

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    There has been an upsurge of interest in the phytobiotics coincident with the onset of the potential ban on the use of antibiotic growth promoters (AGPs) in the broiler industry and because many kinds of nutraceuticals play an important role in improving growth performance, feed efficiency, and gut health of broilers. In the previous years, significant biological activities of essential oils (EOs) belonging to phytobiotics were observed, including anti-bacterial, antifungal, antiviral, and antioxidant properties. We found new perspectives on the roles of EOs, particularly extracts from the Apiaceae family, which is one of the largest plant families, in potential replacement of AGPs, and on the chemical composition involved in regulating microorganism activity and oxidative damage. Furthermore, the positive effects of EOs on broiler production and the possible mechanisms inducing the involvement of gut health and growth performance have been studied.

  14. Oleuropein suppresses mitochondrial reactive oxygen species generation possibly via an activation of transient receptor potential V1 and sirtuin‐1 in cultured chicken muscle cells 国際誌 査読有り

    Hikaru Muroi, Kouhei Hori, Yukako Tokutake, Yuki Hakamata, Fuminori Kawabata, Masaaki Toyomizu, Motoi Kikusato

    Animal Science Journal 93 (1) e13677 2022年1月

    出版者・発行元:Wiley

    DOI: 10.1111/asj.13677  

    ISSN:1344-3941

    eISSN:1740-0929

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    This study investigated the intracellular mechanism governing the effects of oleuropein (OLE), a phenolic compound of Olea europaea, on mRNA expression of avian uncoupling protein (avUCP) and mitochondrial biogenesis-related factors, and reactive oxygen species (mitROS) generation in a primary cultured chicken muscle cells. The OLE-treated cells exhibited increases in Avucp and ATP5a1z expression and a decrease in mitROS generation (p < 0.05), while the effects was canceled by sirtuin-1 (SIRT1) or transient receptor potential vanilloid 1 (TRPV1) inhibitors, EX-527 or BCTC, respectively. Intracellular Ca2+ concentration was significantly increased by OLE, while the induction was canceled by BCTC. The study also found that TRPV1 was expressed in the cell membrane and endoplasmic reticulum (ER), and Ca2+ could be released from ER in the OLE-treated cells. The OLE-treated cells exhibited increases in the phosphorylation ratio of AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) protein content. EX-527 and BCTC inhibitors canceled the effects of OLE on p-AMPK ratio and PGC-1α content, while EX-527 SIRT did not change PGC-1α content. The results suggest that the OLE effects may be due to Ca2+ release, possibly from TRPV1 at ER, and increased p-AMPK ratio, followed by SIRT1 activation and PGC-1α protein expression.

  15. Comparative study of certain antioxidants-electrolyzed reduced water, tocotrienol and vitamin E in heat-induced oxidative damage and performance in broilers 査読有り

    MAK Azad, M Kikusato, I Zulkifli, MM Rahman, MS Ali, MA Hashem, M Toyomizu

    Meat Research 1 (1) 2021年12月2日

    出版者・発行元:Bangladesh Meat Science Association

    DOI: 10.55002/mr.1.1.7  

    eISSN:2790-1971

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    This study was designed to examine the anti-oxidative effect of electrolyzed reduced water, tocotrienol and vitamin E on heat-induced oxidative damage and performance in an experimental model in broilers. On day 12, broiler chickens were subjected to one of the following dietary groups; (i) basal diet and untreated drinking water (control), (ii) basal diet and electrolyzed reduced drinking water (ERW), (iii) basal diet supplemented with 2% rice bran scum oil (as a source of tocotreinol) and untreated drinking water (TOCO), and (iv) basal diet supplemented with vitamin E at 50 mg/kg and untreated drinking water (VITE). On day 14, chickens were exposed to either 34°C continuously for a period of 14 days, or maintained at 24°C on the same diet. Heat-exposed birds consumed significantly less feed resulting in lower weight gain and feed efficiency compared with birds kept at 24°C. Skeletal muscle and liver MDA levels were significantly increased in heat-exposed control birds. The heat-exposed ERW chicks showed significantly improved growth performance and lower levels of MDA contents in tissues than heat-stressed control broilers. Following heat exposure, TOCO and VITE chicks did not exhibit improved performance, while those chicks significantly reduced oxidative damage to the various organs. The results demonstrate that electrolyzed reduced water, tocotrienol and vitamin E effectively protect heat-induced oxidative damage in broilers but they do not improve growth performance except electrolyzed reduced water treatment.

  16. In vivo emergence of beige-like fat in chickens as physiological adaptation to cold environments. 国際誌 査読有り

    Rina Sotome, Akira Hirasawa, Motoi Kikusato, Taku Amo, Kyohei Furukawa, Anna Kuriyagawa, Kouichi Watanabe, Anne Collin, Hitoshi Shirakawa, Ryota Hirakawa, Yuta Tanitaka, Hideki Takahashi, Guoyao Wu, Tomonori Nochi, Tsuyoshi Shimmura, Craig H Warden, Masaaki Toyomizu

    Amino Acids 53 (3) 381-393 2021年3月

    DOI: 10.1007/s00726-021-02953-5  

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    While it has been hypothesized that brown adipocytes responsible for mammalian thermogenesis are absent in birds, the existence of beige fat has yet to be studied directly. The present study tests the hypothesis that beige fat emerges in birds as a mechanism of physiological adaptation to cold environments. Subcutaneous neck adipose tissue from cold-acclimated or triiodothyronine (T3)-treated chickens exhibited increases in the expression of avian uncoupling protein (avUCP, an ortholog of mammalian UCP2 and UCP3) gene and some known mammalian beige adipocyte-specific markers. Morphological characteristics of white adipose tissues of treated chickens showed increased numbers of both small and larger clusters of multilocular fat cells within the tissues. Increases in protein levels of avUCP and mitochondrial marker protein, voltage-dependent anion channel, and immunohistochemical analysis for subcutaneous neck fat revealed the presence of potentially thermogenic mitochondria-rich cells. This is the first evidence that the capacity for thermogenesis may be acquired by differentiating adipose tissue into beige-like fat for maintaining temperature homeostasis in the subcutaneous fat 'neck warmer' in chickens exposed to a cold environment.

  17. Growth performance responses to increased tryptophan supplementation in growing barrows fed three different very low crude protein corn and soybean meal‐based diets fortified with essential amino acids 査読有り

    Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Marcin Taciak, Motoi Kikusato, Kan Sato, Masaaki Toyomizu

    Animal Science Journal 92 (1) 2021年1月

    出版者・発行元:Wiley

    DOI: 10.1111/asj.13605  

    ISSN:1344-3941

    eISSN:1740-0929

  18. Heat stress directly affects intestinal integrity in broiler chickens 査読有り

    Fumika Nanto-Hara, Motoi Kikusato, Shyuichi Ohwada, Masaaki Toyomizu

    The Journal of Poultry Science 57 (4) 284-290 2020年10月25日

    DOI: 10.2141/jpsa.0190004  

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    A study using pair-feeding technique was conducted to determine whether heat exposure directly or indirectly (via reduced feed intake) increases intestinal mucosal damage and permeability to endotoxin in broiler chickens. Male broiler chickens (Ross 308), 27-d-old, were subjected to one of the three treatments (n=8): 1) thermo-neutral conditions (24°C) with ad libitum feed intake, 2) heat stress conditions (33°C) with ad libitum feed intake, or 3) pair-feeding under thermo-neutral conditions, with the feed intake identical to that of heat-stressed chickens. Using these groups, two experiments were performed to evaluate temporal changes in the intestinal morphology in response to each treatment. In experiment 1, chickens were sacrificed after 24 h of exposure to the treatment conditions, while in experiment 2, chickens were sacrificed after 12 or 72 h of exposure to the treatment conditions. In experiment 1, exposure to heat stress conditions for 24 h significantly decreased both the villus height to crypt depth ratio and number of proliferating cell nuclear antigen (PCNA)-positive cells in the duodenum and increased the plasma endotoxin concentration. These findings were not observed in pair-fed chickens. In experiment 2, intestinal integrity and function were unaffected by 12 h of heat stress. On the other hand, chickens exposed to heat stress for 72 h exhibited significantly damaged intestinal morphology in the duodenum as well as increased plasma endotoxin concentration; these negative effects were not observed in pair-fed chickens. These findings suggest that the intestinal morphology and permeability changes observed in chickens that are heat-stressed for 24-72 h are due to the heat stress conditions and not due to reduced feed intake.

  19. Heat stress causes immune abnormalities via massive damage to effect proliferation and differentiation of lymphocytes in broiler Chickens 国際誌 査読有り

    Ryota Hirakawa, Siti Nurjanah, Kyohei Furukawa, Atsushi Murai, Motoi Kikusato, Tomonori Nochi, Masaaki Toyomizu

    Frontiers in Veterinary Science 7 46-46 2020年

    DOI: 10.3389/fvets.2020.00046  

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    Broiler chickens are highly sensitive to high ambient temperatures due to their feathers, lack of skin sweat glands, and high productivity. Heat stress (HS) is a major concern for the poultry industry because it negatively affects growth as well as immune functions, which increase the potential risk of infectious disease outbreaks. Therefore, it is vital to elucidate HS's effect on the avian immune system, especially considering the global rise in average surface temperature. Our study identified a series of immunological disorders in heat-stressed broiler chickens. We exposed 22-day-old broiler chickens to a continuous HS condition (34.5 ± 0.5°C) for 14 days and immunized them with a prototype bovine serum albumin (BSA) antigen. The plasma and lymphoid tissues (thymus, bursa of Fabricius, and spleen) were harvested at the end of the experiments to investigate the induction of BSA-specific immune responses. Our results revealed that plasma titers of immunoglobulin (Ig)Y, IgM, and IgA antibodies specific for BSA were lower than those of thermoneutral chickens immunized with BSA. Furthermore, the spleens of the heat-stressed broiler chickens displayed severe depression of Bu1+ B cells and CD3+ T cells, including CD4+ T cells and CD8+ T cells, and lacked a fully developed germinal center (GC), which is crucial for B cell proliferation. These immunological abnormalities might be associated with severe depression of CD4-CD8- or CD4+CD8+ cells, which are precursors of either helper or killer T cells in the thymus and Bu1+ B cells in the bursa of Fabricius. Importantly, HS severely damaged the morphology of the thymic cortex and bursal follicles, where functional maturation of T and B cells occur. These results indicate that HS causes multiple immune abnormalities in broiler chickens by impairing the developmental process and functional maturation of T and B cells in both primary and secondary lymphoid tissues.

  20. Correlations between mitochondrial respiration activity and residual feed intake after divergent genetic selection for high and low oxygen consumption in mice 査読有り

    Hongyu DARHAN, Atsushi ZODA, Motoi KIKUSATO, Masaaki TOYOMIZU, Kazuo KATOH, Sang-gun ROH, Shinichiro OGAWA, Yoshinobu UEMOTO, Masahiro SATOH, Keiichi SUZUKI

    Animal Science Journal 90 (7) 818-826 2019年4月

    出版者・発行元:None

    DOI: 10.1111/asj.13210  

    ISSN:1344-3941

    eISSN:1740-0929

  21. Nutritional regulation of mitochondrial ROS production of chickens exposed to acute and chronic heat stress

    M. Toyomizu, A. Mujahid, R. Hirakawa, K. Furukawa, M. A.K. Azad, M. Taciak, M. Kikusato

    EAAP Scientific Series 138 73-81 2019年

    DOI: 10.3920/978-90-8686-891-9_5  

    ISSN:0071-2477

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    Harmful effects of heat stress on organisms are a matter of concern for environmental health and global warming. High ambient temperature, whether it is ‘acute' or ‘chronic' in nature, constitutes a significant hindrance to the growth of animals. Heat-induced reactive oxygen species (ROS) formation may be a factor that causes molecular changes in DNA, proteins, lipids and other biological molecules. To create a method for nutritional regulation of ROS production and protein degradation of skeletal muscle in heat-stressed chickens, we focused on elucidating underlying effects of heat stress on ROS production of skeletal muscle with two models to identify conditions for making broiler chickens more tolerant to heat stress. ‘Acute' heat stress in broiler chickens under 34 °C conditions induces increased mitochondrial ROS production via increased β-oxidation and downregulation of the avian form of mitochondrial uncoupling protein (avUCP), resulting in higher oxidative damage to mitochondrial proteins and lipids. Similarly, ‘chronic' heat stress induces increased ROS production in skeletal muscle mitochondria, probably via elevation of the membrane potential DY in state 4, resulting from enhanced oxygen consumption in the initial stage of heat exposure. However, animals can become acclimatized to environmental heat stress. Muscle protein degradation can occur after a short time (3 d) after heat exposure and this may be due to the activation of ubiquitination by atrogin-1 involved with mitochondrial ROS production. Nutritional regulation of ROS production and protein degradation, such as via enhanced avUCP gene expression, are important for making broiler chickens more tolerant to heat stress.

  22. Selection for high and low oxygen consumption-induced differences in maintenance energy requirements of mice. 国際誌 査読有り

    Hongyu Darhan, Motoi Kikusato, Masaaki Toyomizu, Sang-Gun Roh, Kazuo Katoh, Masahiro Sato, Keiichi Suzuki

    Animal Science Journal 88 (7) 959-965 2017年7月

    出版者・発行元:WILEY

    DOI: 10.1111/asj.12740  

    ISSN:1344-3941

    eISSN:1740-0929

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    Maintenance energy requirements (MER) of mice selected for high (H) or low (L) oxygen consumption (OC) were compared. Forty-four mice from H and L OC lines were weaned at 3 weeks and divided into four experimental groups: group A were sacrificed at 4 weeks; group B were fed ad libitum, and groups C and D were fed 2.8 and 2.4 g/day, respectively, from 4 to 8 weeks of age. Groups B-D were sacrificed at 8 weeks. Chemical components were estimated for all groups. MER was estimated using a model that partitioned metabolizable energy intake into that used for maintenance, and protein and fat deposition. The feed conversion ratio for the B group was significantly higher in the H than in the L line. Feed intake for metabolic energy content per metabolic body size was significantly also higher in the H line, whereas accumulated energy content per metabolic body size was significantly higher in the L line. MER of the H line was greater than that of the L line (P < 0.10). These results suggest that selection for H or L OC produced differences in chemical components, feed efficiency, and MER between the H and L lines.

  23. Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases. 国際誌 査読有り

    Tetsuro Matsuhashi, Takeya Sato, Shin-Ichiro Kanno, Takehiro Suzuki, Akihiro Matsuo, Yuki Oba, Motoi Kikusato, Emi Ogasawara, Tai Kudo, Kosuke Suzuki, Osamu Ohara, Hiroko Shimbo, Fumika Nanto, Hiroaki Yamaguchi, Daisuke Saigusa, Yasuno Mukaiyama, Akiko Watabe, Koichi Kikuchi, Hisato Shima, Eikan Mishima, Yasutoshi Akiyama, Yoshitsugu Oikawa, H O Hsin-Jung, Yukako Akiyama, Chitose Suzuki, Mitsugu Uematsu, Masaki Ogata, Naonori Kumagai, Masaaki Toyomizu, Atsushi Hozawa, Nariyasu Mano, Yuji Owada, Setsuya Aiba, Teruyuki Yanagisawa, Yoshihisa Tomioka, Shigeo Kure, Sadayoshi Ito, Kazuto Nakada, Ken-Ichiro Hayashi, Hitoshi Osaka, Takaaki Abe

    EBioMedicine 20 27-38 2017年6月

    DOI: 10.1016/j.ebiom.2017.05.016  

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    Mitochondrial dysfunction increases oxidative stress and depletes ATP in a variety of disorders. Several antioxidant therapies and drugs affecting mitochondrial biogenesis are undergoing investigation, although not all of them have demonstrated favorable effects in the clinic. We recently reported a therapeutic mitochondrial drug mitochonic acid MA-5 (Tohoku J. Exp. Med., 2015). MA-5 increased ATP, rescued mitochondrial disease fibroblasts and prolonged the life span of the disease model "Mitomouse" (JASN, 2016). To investigate the potential of MA-5 on various mitochondrial diseases, we collected 25 cases of fibroblasts from various genetic mutations and cell protective effect of MA-5 and the ATP producing mechanism was examined. 24 out of the 25 patient fibroblasts (96%) were responded to MA-5. Under oxidative stress condition, the GDF-15 was increased and this increase was significantly abrogated by MA-5. The serum GDF-15 elevated in Mitomouse was likewise reduced by MA-5. MA-5 facilitates mitochondrial ATP production and reduces ROS independent of ETC by facilitating ATP synthase oligomerization and supercomplex formation with mitofilin/Mic60. MA-5 reduced mitochondria fragmentation, restores crista shape and dynamics. MA-5 has potential as a drug for the treatment of various mitochondrial diseases. The diagnostic use of GDF-15 will be also useful in a forthcoming MA-5 clinical trial.

  24. The suppressive effect of dietary coenzyme Q10 on mitochondrial reactive oxygen species production and oxidative stress in chickens exposed to heat stress. 国際誌 査読有り

    Motoi Kikusato, Kasumi Nakamura, Yukiko Mikami, Ahmad Mujahid, Masaaki Toyomizu

    Animal Science Journal 87 (10) 1244-1251 2016年10月

    出版者・発行元:WILEY

    DOI: 10.1111/asj.12543  

    ISSN:1344-3941

    eISSN:1740-0929

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    This study was conducted to determine if dietary supplementation with coenzyme Q10 (CoQ10 ), which can act as a potent antioxidant and is an obligatory cofactor of mitochondrial uncoupling protein, suppresses the heat stress (HS)-induced overproduction of mitochondrial reactive oxygen species (ROS) and oxidative damage in the skeletal muscle of birds. The carbonyl protein content of skeletal muscle was significantly higher in birds exposed to HS treatment (34°C, 12 h) than in thermoneutral birds (25°C). This increase was suppressed by CoQ10 supplementation (40 mg/kg diet). Succinate-supported mitochondrial ROS production was increased by HS treatment, and this increase was also suppressed by CoQ10 supplementation. In contrast, CoQ10 supplementation did not affect the HS-induced decrease in mitochondrial proton leak. The mitochondrial membrane potential (ΔΨ), to which HS-induced ROS production was previously shown to be sensitive, tended to be increased by HS treatment, but this rise in ΔΨ was not affected by CoQ10 supplementation. Taken together, these results suggest that dietary CoQ10 supplementation attenuates HS-induced oxidative damage to skeletal muscle, by preventing the overproduction of succinate-supported mitochondrial ROS in a manner that is independent of ΔΨ. © 2015 Japanese Society of Animal Science.

  25. Mitochonic Acid 5 Binds Mitochondria and Ameliorates Renal Tubular and Cardiac Myocyte Damage. 国際誌 査読有り

    Takehiro Suzuki, Hiroaki Yamaguchi, Motoi Kikusato, Osamu Hashizume, Satoru Nagatoishi, Akihiro Matsuo, Takeya Sato, Tai Kudo, Tetsuro Matsuhashi, Kazutaka Murayama, Yuki Ohba, Shun Watanabe, Shin-Ichiro Kanno, Daichi Minaki, Daisuke Saigusa, Hiroko Shinbo, Nobuyoshi Mori, Akinori Yuri, Miyuki Yokoro, Eikan Mishima, Hisato Shima, Yasutoshi Akiyama, Yoichi Takeuchi, Koichi Kikuchi, Takafumi Toyohara, Chitose Suzuki, Takaharu Ichimura, Jun-Ichi Anzai, Masahiro Kohzuki, Nariyasu Mano, Shigeo Kure, Teruyuki Yanagisawa, Yoshihisa Tomioka, Masaaki Toyomizu, Kohei Tsumoto, Kazuto Nakada, Joseph V Bonventre, Sadayoshi Ito, Hitoshi Osaka, Ken-Ichi Hayashi, Takaaki Abe

    Journal of the American Society of Nephrology : JASN 27 (7) 1925-32 2016年7月

    出版者・発行元:AMER SOC NEPHROLOGY

    DOI: 10.1681/ASN.2015060623  

    ISSN:1046-6673

    eISSN:1533-3450

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    Mitochondrial dysfunction causes increased oxidative stress and depletion of ATP, which are involved in the etiology of a variety of renal diseases, such as CKD, AKI, and steroid-resistant nephrotic syndrome. Antioxidant therapies are being investigated, but clinical outcomes have yet to be determined. Recently, we reported that a newly synthesized indole derivative, mitochonic acid 5 (MA-5), increases cellular ATP level and survival of fibroblasts from patients with mitochondrial disease. MA-5 modulates mitochondrial ATP synthesis independently of oxidative phosphorylation and the electron transport chain. Here, we further investigated the mechanism of action for MA-5. Administration of MA-5 to an ischemia-reperfusion injury model and a cisplatin-induced nephropathy model improved renal function. In in vitro bioenergetic studies, MA-5 facilitated ATP production and reduced the level of mitochondrial reactive oxygen species (ROS) without affecting activity of mitochondrial complexes I-IV. Additional assays revealed that MA-5 targets the mitochondrial protein mitofilin at the crista junction of the inner membrane. In Hep3B cells, overexpression of mitofilin increased the basal ATP level, and treatment with MA-5 amplified this effect. In a unique mitochondrial disease model (Mitomice with mitochondrial DNA deletion that mimics typical human mitochondrial disease phenotype), MA-5 improved the reduced cardiac and renal mitochondrial respiration and seemed to prolong survival, although statistical analysis of survival times could not be conducted. These results suggest that MA-5 functions in a manner differing from that of antioxidant therapy and could be a novel therapeutic drug for the treatment of cardiac and renal diseases associated with mitochondrial dysfunction.

  26. Time-course changes in muscle protein degradation in heat-stressed chickens: Possible involvement of corticosterone and mitochondrial reactive oxygen species generation in induction of the ubiquitin-proteasome system. 国際誌 査読有り

    Kyohei Furukawa, Motoi Kikusato, Tomomi Kamizono, Masaaki Toyomizu

    General and Comparative Endocrinology 228 105-110 2016年3月1日

    出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE

    DOI: 10.1016/j.ygcen.2016.02.007  

    ISSN:0016-6480

    eISSN:1095-6840

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    Heat stress (HS) induces muscle protein degradation as well as production of mitochondrial reactive oxygen species (ROS). In the present study, to improve our understanding of how protein degradation is induced by HS treatment in birds, a time course analysis of changes in the circulating levels of glucocorticoid and N(τ)-methylhistidine, muscle proteolysis-related gene expression, and mitochondrial ROS generation, was conducted. At 25 days of age, chickens were exposed to HS conditions (33 °C) for 0, 0.5, 1 or 3 days. While no alteration in plasma N(τ)-methylhistidine concentration relative to that of the control group was observed in the 0.5 day HS group, the concentration was significantly higher in the 3-d HS treatment group. Plasma corticosterone concentrations increased in response to 0.5-d HS treatment, but subsequently returned to near-normal values. HS treatment for 0.5 days did not change the levels of μ-calpain, cathepsin B, or proteasome C2 subunit mRNA, but increased the levels of mRNA encoding atrogin-1 (P<0.05) and its transcription factor, forkhead box O3 (P=0.09). Under these hyperthermic conditions, mitochondrial superoxide production was significantly increased than that of thermoneutral control. Here, we show that HS-induced muscle protein degradation may be due to the activation of ubiquitination by atrogin-1, and that this process may involve mitochondrial ROS production as well as corticosterone secretion.

  27. Effect of feeding diets combining whole-grain paddy rice and high levels of fat on broiler chicken growth 査読有り

    Fumika Nanto, Chiaki Ito, Motoi Kikusato, Masaaki Toyomizu

    The Journal of Poultry Science 53 (1) 34-39 2016年1月25日

    出版者・発行元:JAPAN POULTRY SCIENCE ASSOC

    DOI: 10.2141/jpsa.0150070  

    ISSN:1346-7395

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    Previously, we showed that the growth of chicks fed a diet containing 43% whole-grain paddy rice and 10% soybean oil was retarded relative to a control group fed a corn-based diet containing 6% soybean oil. However, feeding chicks with 43% whole-grain paddy rice containing 6% soybean oil resulted in normal growth. It is possible that the observed growth retardation was caused by the high soybean oil content or resulted from the combination of whole-grain paddy rice and the high level of soybean oil which was added to the diet to maintain the overall energy content. The present study was therefore carried out to identify the reasons for the observed growth retardation. Thirty-six chicks (0-day-old) were divided into six equal-sized groups that were fed one of the following six experimental diets ad libitum for 28 d: two kinds of dehulled rice-based diets containing 5% or 10% soybean oil (DS5% or DS10%), another three whole-grain paddy rice-based diets containing 10% soybean oil, corn oil, or rendering oil (WS10%, WC10%, WR10%, respectively), and a WS10% diet supplemented with vitamin B12, methionine and ethoxyquin. The body weight gain of groups fed the WS10% and WC10% diets was significantly lower than the weight gain of birds fed the DS5% diet (control). In addition, the liver of birds fed the WS10% and WC10% diet exhibited significantly higher lipid peroxidation than that of the control group. In comparison, supplementation of the WS10% diet with vitamin B12, methionine and ethoxyquin dramatically improved growth and hepatic oxidation status. These results indicate that diets combining whole-grain paddy rice and high levels of soybean and corn oil adversely affect performance, presumably via lipid peroxidation in the liver.

  28. ニワトリ骨格筋におけるミトコンドリアの酸化的リン酸化および活性酸素産生の代謝調節能 査読有り

    喜久里 基, 古川 恭平, 神園 巴美, 袴田 祐基, 豊水 正昭

    栄養生理研究会報 = Proceedings of Japanese Society for Animal Nutrition and Metabolism 60 (2) 57-68 2016年

    出版者・発行元:家畜栄養生理研究会

    ISSN:0286-4754

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    ニワトリ骨格筋におけるミトコンドリアの酸化的リン酸化および活性酸素産生の代謝調節能

  29. Moderate dependence of reactive oxygen species production on membrane potential in avian muscle mitochondria oxidizing glycerol 3-phosphate. 査読有り

    Motoi Kikusato, Masaaki Toyomizu

    The Journal of Physiological Sciences 65 (6) 555-9 2015年11月

    出版者・発行元:SPRINGER JAPAN KK

    DOI: 10.1007/s12576-015-0395-2  

    ISSN:1880-6546

    eISSN:1880-6562

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    Mitochondria are a major source of reactive oxygen species production in cells, and the production level is sensitive to the magnitude of the membrane potential (ΔΨ). The present study investigated the level of superoxide production in mitochondria oxidizing glycerol 3-phosphate (GP) and its dependence on ΔΨ in isolated avian muscle mitochondria. The levels of superoxide produced in mitochondria oxidizing GP were lower than those obtained with succinate and were similar to those obtained with NADH-linked substrates (glutamate/malate/pyruvate). The dependence of superoxide production on ΔΨ in mitochondria oxidizing GP was lower than that of mitochondria oxidizing succinate, and a weak dependence of GP-supported superoxide production on ΔΨ was observed in the presence of NADH-linked substrates or succinate. These results suggest that the levels of superoxide generated in response to GP are quantitatively low, but they are unsusceptible to changes in ΔΨ in avian muscle mitochondria.

  30. Possible involvement of mitochondrial reactive oxygen species production in protein degradation induced by heat stress in avian muscle cells 査読有り

    Kyohei Furukawa, Motoi Kikusato, Tomomi Kamizono, Hayami Yoshida, Masaaki Toyomizu

    The Journal of Poultry Science 52 (4) 260-267 2015年10月25日

    出版者・発行元:JAPAN POULTRY SCIENCE ASSOC

    DOI: 10.2141/jpsa.0150028  

    ISSN:1346-7395

    eISSN:1349-0486

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    � 2015, Japan Poultry Science Association. Heat stress (HS) stimulates mitochondrial reactive oxygen species (ROS) production and protein degradation in skeletal muscle. The present study investigated the stimulatory effects of HS-induced mitochondrial ROS production on the ubiquitin-proteasome protein degradation system in primary cultured avian muscle cells. Cells were isolated from the breast muscle of neonatal chicks, and then grown for 48 h. Thereafter, the cells were subjected to 37�C or 41�C (HS). Exposure to 6 h of HS treatment significantly decreased the cellular protein content compared to that of normal cells, an effect was completely suppressed by the addition of a proteasome-specific inhibitor. Whereas the mRNA levels of the 20S proteasome C2 subunit, which is one of the subunits of the 26S proteasome, did not change at any time during HS treatment (1, 3, 6 h), the mRNA levels of atrogin-1 and muscle ring-finger protein 1, both of which are muscle-specific ubiquitin ligases, increased after 1 h of HS but then decreased to near-normal values with time. Intracellular ROS production (the sum of H2O2, hydroxyl radicals, peroxyl radicals, peroxynitrite) did not change in the 1 h HS-exposed cells, but was significantly increased after 3 h and 6 h of HS. Mitochondrial superoxide production was significantly increased after 1 h of HS, which might increase the mRNA expression of ubiquitin ligase in muscle cells. In cells pretreated with 4-hydroxy TEMPO, which is able to decrease mitochondrial superoxide production, the increases in mitochondrial superoxide production and ubiquitin ligase mRNA levels observed after 1 h of HS were suppressed. The protein content of these cells was not decreased, which was observed after the longest period of HS (6 h). These findings suggest that mitochondrial superoxide production may play an important role in activating the ubiquitin-proteasome system, probably via the induction of ubiquitin ligases, in HS-exposed muscle cells.

  31. Selection for high and low oxygen consumption altered hepatic mitochondrial energy efficiency in mice 査読有り

    Yu Hong, Astrid Ardiyanti, Motoi Kikusato, Tomoyuki Shimazu, Masaaki Toyomizu, Keiichi Suzuki

    Animal Science Journal 86 (9) 818-825 2015年9月

    出版者・発行元:WILEY-BLACKWELL

    DOI: 10.1111/asj.12366  

    ISSN:1344-3941

    eISSN:1740-0929

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    Selection for high (H) and low (L) oxygen consumption (OC) as an indirect estimation of maintenance energy requirement was determined. Feed intake and body weight were measured and feed conversion ratio (FCR) of 4-8-week-old mice was calculated. Respiratory activity of hepatic mitochondria was measured at 12 weeks. Total feed intake (H: 103.74g, L: 97.92g, P&lt;0.01), daily feed intake (H: 3.70g/day, L: 3.50g/day, P&lt;0.01) and FCR (H: 18.79, L: 15.50, P&lt;0.01) were significantly different between lines. The line by sex interaction was significant for FCR. No line differences were observed in males; and the FCR of the H line was greater than in the L line in females. H line mice had the highest hepatic mitochondrial respiratory activity in state 2 (P&lt;0.01), the highest uncoupled respiratory rate of mitochondria in the presence of an uncoupling agent (P&lt;0.001), and the mitochondrial proton leak. The adenosine diphosphate/ O ratio was highest in the L line (P&lt;0.05). This suggests that the selection for high and low OC induced differences in basal mitochondrial respiration and basal metabolism, resulting in difference in FCR between H and L lines.

  32. Effect of heat stress-induced production of mitochondrial reactive oxygen species on NADPH oxidase and heme oxygenase-1 mRNA levels in avian muscle cells. 国際誌 査読有り

    Motoi Kikusato, Hayami Yoshida, Kyohei Furukawa, Masaaki Toyomizu

    Journal of Thermal Biology 52 8-13 2015年8月

    出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD

    DOI: 10.1016/j.jtherbio.2015.04.005  

    ISSN:0306-4565

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    Heat stress is a major factor inducing oxidative disturbance in cells. In the present study, we investigated the mechanism of overproduction of reactive oxygen species (ROS) in cultured avian muscle cells in response to heat stress, and also focused attention on the interaction of mitochondrial superoxide anions with altered NADPH oxidase (NOX), superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) mRNA levels in heat-stressed cells. Exposure of cells to heat stress conditions (41°C, 6h) resulted in increased mitochondrial superoxide and intracellular ROS levels, and increased carbonyl protein content as compared with that of normal cells (37°C). The mitochondrial uncoupler 2,4-dinitrophenol lowered intracellular ROS levels in heat-stressed cells. Heat stress increased NOX4 mRNA and decreased HO-1 mRNA levels, while SOD1 and SOD2 mRNA levels remained relatively stable in heat-stressed cells. Addition of the superoxide scavenger 4-hydroxy TEMPO to the culture medium of heat-stressed cells restored mitochondrial superoxide and intracellular ROS levels as well as NOX4 and HO-1 mRNA levels to near-normal values. We suggest that mitochondrial superoxide production could play an influential role in augmenting oxidative damage to avian muscle cells, possibly via the up-regulation of NOX4 and down-regulation of HO-1 in heat-stressed avian muscle cells.

  33. Effect of feeding butoxybutyl alcohol on the growth performance and status of skeletal muscle proteolysis in broiler chickens 査読有り

    T. Kamizono, D. Saputra, I. Miura, M. Kikusato, K. Hayashi, M. Toyomizu

    Journal of Agricultural Science 153 (5) 920-928 2015年7月

    出版者・発行元:CAMBRIDGE UNIV PRESS

    DOI: 10.1017/S0021859615000039  

    ISSN:0021-8596

    eISSN:1469-5146

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    Butoxybutyl alcohol (BBA) is a possible growth promoter contained in the fermentation and distillation by-products of a traditional Japanese spirit, shochu. In the present study, BBA was synthesized and its chemical structure was confirmed by gas chromatography mass spectrometry and nuclear magnetic resonance. Then, two studies were conducted to investigate the effects of feeding the synthesized BBA on the growth and skeletal muscle proteolysis of broiler chickens. Ross male broiler chickens were divided into two groups, control (basal diet: 219g crude protein/kg and 12.66MJ metabolizable energy/kg) and BBA diet (30mg BBA/kg basal diet), with the experimental diets being provided from 15 to 27 days and 0 to 27 days of age, for Studies 1 and 2, respectively. Butoxybutyl alcohol supplementation increased final body weight in both studies, whereas feed intake was unchanged, thereby indicating significantly increased feed efficiency. Furthermore, the synthesized BBA increased the weights of the pectoralis superficialis and profundus muscles, and the leg. The BBA decreased the N-methylhistidine concentration in the excrement and plasma, which are indices of the rate of skeletal muscle protein degradation. It also decreased the mRNA levels of -calpain large subunit, atrogin-1/muscle atrophy F-box (MAFbx), ubiquitin and 20S proteasome C2 subunit. These suggest that growth promotion due to the feeding of synthesized BBA is caused by the suppression of skeletal muscle protein degradation, which is related to a decrease in gene expression in the calpain and ubiquitin-proteasome systems.

  34. Effects of Whole-Grain Paddy Rice on Growth Performance, Oxidative Stress and Morphological Alterations of the Intestine in Broiler Chickens Exposed to Acute and Chronic Heat Stress 査読有り

    Fumika Nanto, Chiaki Ito, Motoi Kikusato, Masaaki Toyomizu

    The Journal of Poultry Science 52 (2) 109-118 2015年4月

    出版者・発行元:JAPAN POULTRY SCIENCE ASSOC

    DOI: 10.2141/jpsa.0140009  

    ISSN:1346-7395

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    We have previously shown that, under thermoneutral conditions, the growth performance of chicks fed a rice-based diet containing 6% soybean oil [metabolizable energy (ME): 2,800 kcal/kg] for 28 days was comparable to that obtained with a corn-based diet containing 6% soybean oil (ME: 3,100 kcal/kg). However, there is no information available concerning how such diets compare with respect to growth performance under heat stress conditions. The present study was therefore conducted to clarify differences in dietary effectiveness between corn-based and whole-grain paddy rice-based diets [formulated to be equivalent in terms of fat (6%), but not iso-caloric] under the two conditions of acute and chronic heat stress (Experiment 1 and Experiment 2, respectively). In Experiment 1, the body weight gain of a corn- and rice-fed group of chickens exposed to acute heat (33 degrees C-12 h) were significantly decreased compared with that of a corn-fed control group (24 degrees C), with similar results obtained for the two heat-treated groups. Malondialdehyde (MDA) levels in the M. pectoralis superficialis were significantly increased in corn-fed birds exposed to acute heat stress, but not in the rice-fed group (33 degrees C-12 h). In Experiment 2, body weight gains in the corn- and rice-fed groups exposed to chronic heat (33 degrees C-6 d) were significantly decreased, and to a similar extent, compared with the corn-fed control group (24 degrees C). Muscle MDA levels in the corn- and rice-fed groups were similarly increased by the chronic heat exposure compared with the control group. These results suggest that, in response to acute heat stress (but not chronic heat stress) conditions, the feeding of whole-grain paddy rice diet to chickens may attenuate skeletal muscle oxidative damage compared with that in corn-fed chickens. The possible involvement of intestinal morphology (such as the villus height: crypt depth ratio observed here) on growth performance is also discussed.

  35. Methionine deficiency leads to hepatic fat accretion via impairment of fatty acid import by carnitine palmitoyltransferase I 査読有り

    M. Kikusato, S. Sudo, M. Toyomizu

    British Poultry Science 56 (2) 225-231 2015年3月

    出版者・発行元:TAYLOR & FRANCIS LTD

    DOI: 10.1080/00071668.2014.996529  

    ISSN:0007-1668

    eISSN:1466-1799

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    1. To clarify the underlying mechanism of hepatic fat accretion due to methionine (Met) deficiency in broiler chickens, the present study investigated the effect of Met deficiency on the hepatic carnitine palmitoyltransferase (CPT) system, which imports fatty acids into mitochondria. 2. Fifteen-d-old male meat-type chickens were fed on either a control diet (containing 0.52g/100gMet) or a Met-deficient diet (containing 0.27gMet/100g). After a 10-d feeding period, the birds were killed by decapitation and their livers excised to determine hepatic CPT1 and CPT2 mRNA levels and for the related hepatic fatty acid-supported mitochondrial respiration to be measured. 3. Met deficiency decreased body weight gain and feed efficiency and increased hepatic lipid content compared to the control group. Whereas the hepatic CPT2 mRNA level in the Met-deficient group remained unchanged compared to that of the control group, the CPT1 mRNA level was decreased in the Met-deficient group and CPT1-dependent hepatic mitochondrial respiration was impaired. 4. Our results suggest that the hepatic lipid accretion that occurs in response to Met deficiency might be attributable to the impairment of CPT1-mediated fatty acid import into mitochondria.

  36. Mitochonic Acid 5 (MA-5), a Derivative of the Plant Hormone Indole-3-Acetic Acid, Improves Survival of Fibroblasts from Patients with Mitochondrial Diseases 査読有り

    Suzuki Takehiro, Yamaguchi Hiroaki, Kikusato Motoi, Matsuhashi Tetsuro, Matsuo Akihiro, Sato Takeya, Oba Yuki, Watanabe Shun, Minaki Daichi, Saigusa Daisuke, Shimbo Hiroko, Mori Nobuyoshi, Mishima Eikan, Shima Hisato, Akiyama Yasutoshi, Takeuchi Yoichi, Yuri Akinori, Kikuchi Koichi, Toyohara Takafumi, Suzuki Chitose, Kohzuki Masahiro, Anzai Jun-ichi, Mano Nariyasu, Kure Shigeo, Yanagisawa Teruyuki, Tomioka Yoshihisa, Toyomizu Masaaki, Ito Sadayoshi, Osaka Hitoshi, Hayashi Ken-ichiro, Abe Takaaki

    The Tohoku Journal of Experimental Medicine 236 (3) 225-232 2015年

    出版者・発行元:Tohoku University Medical Press

    DOI: 10.1620/tjem.236.225  

    ISSN:0040-8727

    eISSN:1349-3329

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    Mitochondria are key organelles implicated in a variety of processes related to energy and free radical generation, the regulation of apoptosis, and various signaling pathways. Mitochondrial dysfunction increases cellular oxidative stress and depletes ATP in a variety of inherited mitochondrial diseases and also in many other metabolic and neurodegenerative diseases. Mitochondrial diseases are characterized by the dysfunction of the mitochondrial respiratory chain, caused by mutations in the genes encoded by either nuclear DNA or mitochondrial DNA. We have hypothesized that chemicals that increase the cellular ATP levels may ameliorate the mitochondrial dysfunction seen in mitochondrial diseases. To search for the potential drugs for mitochondrial diseases, we screened an in-house chemical library of indole-3-acetic-acid analogs by measuring the cellular ATP levels in Hep3B human hepatocellular carcinoma cells. We have thus identified mitochonic acid 5 (MA-5), 4-(2,4-difluorophenyl)-2-(1H-indol-3-yl)-4-oxobutanoic acid, as a potential drug for enhancing ATP production. MA-5 is a newly synthesized derivative of the plant hormone, indole-3-acetic acid. Importantly, MA-5 improved the survival of fibroblasts established from patients with mitochondrial diseases under the stress-induced condition, including Leigh syndrome, MELAS (myopathy encephalopathy lactic acidosis and stroke-like episodes), Leber's hereditary optic neuropathy, and Kearns-Sayre syndrome. The improved survival was associated with the increased cellular ATP levels. Moreover, MA-5 increased the survival of mitochondrial disease fibroblasts even under the inhibition of the oxidative phosphorylation or the electron transport chain. These data suggest that MA-5 could be a therapeutic drug for mitochondrial diseases that exerts its effect in a manner different from anti-oxidant therapy.

  37. Effect of paddy rice diets on intestinal morphology and inflammatory responses in broiler chickens under chronic heat stress conditions 査読有り

    Nanto, F, Kikusato, M, Ohwada, S, Takahashi, K, Toyomizu, M

    The 10th Asian Pacific Poultry Conference 2014年10月

  38. Oleuropein, one of polyphenols in olive oil, attenuates heat stress-induced overproduction of mitochondrial ROS in skeletal muscle of birds 査読有り

    Kikusato, M, Uwabe, Y, Toyomizu, M

    14th European Poultry Conference (EPC2014) 2014年6月

  39. The heat-induced production of reactive oxygen species regulates protein content in cultured chick skeletal muscle cells 査読有り

    Yoshida H, Kikusato M, Souma K, Toyomizu M

    The International Symposium on Energy and Protein Metabolism and Nutrition(ISEP) 134 309-310 2013年9月

  40. Heat stress-induced overproduction of mitochondrial ROS is down-regulated in laying-type chickens 査読有り

    Kikusato M, Toyomizu M

    The International Symposium on Energy and Protein Metabolism and Nutrition(ISEP) 134 267-268 2013年9月

  41. Electrolysed reduced water decreases reactive oxygen species-induced oxidative damage to skeletal muscle and improves performance in broiler chickens exposed to medium-term chronic heat stress 査読有り

    M. A. K. Azad, M. Kikusato, I. Zulkifli, M. Toyomizu

    British Poultry Science 54 (4) 503-509 2013年8月

    出版者・発行元:TAYLOR & FRANCIS LTD

    DOI: 10.1080/00071668.2013.801067  

    ISSN:0007-1668

    eISSN:1466-1799

    詳細を見る 詳細を閉じる

    1. The present study was designed to achieve a reduction of reactive oxygen species (ROS)-induced oxidative damage to skeletal muscle and to improve the performance of broiler chickens exposed to chronic heat stress. 2. Chickens were given a control diet with normal drinking water, or diets supplemented with cashew nut shell liquid (CNSL) or grape seed extract (GSE), or a control diet with electrolysed reduced water (ERW) for 19 d after hatch. Thereafter, chickens were exposed to a temperature of either 34 degrees C continuously for a period of 5 d, or maintained at 24 degrees C, on the same diets. 3. The control broilers exposed to 34 degrees C showed decreased weight gain and feed consumption and slightly increased ROS production and malondialdehyde (MDA) concentrations in skeletal muscle. The chickens exposed to 34 degrees C and supplemented with ERW showed significantly improved growth performance and lower ROS production and MDA contents in tissues than control broilers exposed to 34 degrees C. Following heat exposure, CNSL chickens performed better with respect to weight gain and feed consumption, but still showed elevated ROS production and skeletal muscle oxidative damage. GSE chickens did not exhibit improved performance or reduced skeletal muscle oxidative damage. 4. In conclusion, this study suggests that ERW could partially inhibit ROS-induced oxidative damage to skeletal muscle and improve growth performance in broiler chickens under medium-term chronic heat treatment.

  42. Crucial role of membrane potential in heat stress-induced overproduction of reactive oxygen species in avian skeletal muscle mitochondria 査読有り

    Motoi Kikusato, Masaaki Toyomizu

    PLoS ONE 8 (5) e64412 2013年5月9日

    DOI: 10.1371/journal.pone.0064412  

    ISSN:1932-6203

    詳細を見る 詳細を閉じる

    Heat stress is an environmental factor that causes oxidative stress. We found previously that acute heat stress stimulates the production of reactive oxygen species (ROS) in the skeletal muscle mitochondria of birds, and that this was accompanied by an increase of the mitochondrial membrane potential (ΔΨ) due to increased substrate oxidation by the electron transport chain. We also showed that avian uncoupling protein (avUCP) expression is decreased by the heat exposure. The present study clarifies whether ΔΨ is a major determinant of the overproduction of ROS due to acute heat stress, and if the decrease in avUCP expression is responsible for the elevation in ΔΨ. Control (24°C) and acute heat-stressed (34°C for 12 h) birds exhibited increased succinate-driven mitochondrial ROS production as indicated by an elevation of ΔΨ, with this increase being significantly higher in the heat-stressed group compared with the control group. In glutamate/malate-energized mitochondria, no difference in the ROS production between the groups was observed, though the mitochondrial ΔΨ was significantly higher in the heat-stressed groups compared with the control group. Furthermore, mitochondria energized with either succinate/glutamate or succinate/malate showed increased ROS production and ΔΨ in the heat-stressed group compared with mitochondria from the control group. These results suggest that succinate oxidation could play an important role in the heat stress-induced overproduction of mitochondrial ROS in skeletal muscle. In agreement with the notion of a decrease in avUCP expression in response to heat stress, proton leak, which was likely mediated by UCP (that part which is GDP-inhibited and arachidonic acid-sensitive), was reduced in the heat-exposed group. We suggest that the acute heat stress-induced overproduction of mitochondrial ROS may depend on ΔΨ, which may in turn result not only from increased substrate oxidation but also from a decrease in the mitochondrial avUCP content. © 2013 Kikusato, Toyomizu.

  43. Effects of dehulled, crushed and untreated whole-grain paddy rice on growth performance in Broiler Chickens 査読有り

    Fumika Nanto, Motoi Kikusato, Chiaki Ito, Shota Sudo, Masaaki Toyomizu

    The Journal of Poultry Science 49 (4) 291-299 2012年10月

    出版者・発行元:JAPAN POULTRY SCIENCE ASSOC

    DOI: 10.2141/jpsa.0120049  

    ISSN:1346-7395

    詳細を見る 詳細を閉じる

    Experiments were conducted to determine the effects of different types of paddy rice (dehulled, crushed and untreated whole-grain forms) on growth performance in broiler chickens. In Experiment 1, a control diet containing 41.6% corn (control), and three experimental diets containing 40.7% dehulled, 43% crushed or 43% whole-grain paddy rice, were formulated to be iso-caloric (3,100 kcal/kg ME) and iso-nitrogenous (20% crude protein), but to contain different levels of fat (6%, 5.6%, 10.7%, 10.7%, respectively). Groups of 0-day-old chickens were the fed experimental diets ad libitum for 28 d. The average final body weight of groups fed the crushed and untreated whole-grain paddy rice was significantly lower (P&lt;0.05) than that of the control group, but that of dehulled rice-fed group was slightly higher than the control group. This finding implies that the growth retardation seen with crushed or untreated whole rice could be caused by the feed intake-reduction due to the fat addition to the experimental diets. In Experiment 2, the control diet containing corn and the experimental diets containing dehulled and untreated whole-grain paddy rice were formulated to contain similar levels of fat (5.6-6%) and to be iso-nitrogenous (20%), but not to be iso-caloric. As a result, the average body weight gain of the whole-grain rice-fed chickens showed a considerable increase compared with the control diet-fed chickens even though the energy content of the former diet was only about 90% that of the control diet. It is concluded that whole-grain paddy rice could serve as a valuable constituent of broiler chicken feed, but that excess fat in the diet to maintain the ME content (3,100 kcal/g) could have a negative effect on growth performance.

  44. Meat-type chickens have a higher efficiency of mitochondrial oxidative phosphorylation than laying-type chickens 査読有り

    Masaaki Toyomizu, Motoi Kikusato, Yusuke Kawabata, Md. Abul Kalam Azad, Eriko Inui, Taku Amo

    Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiolog 159 (1) 75-81 2011年5月

    出版者・発行元:ELSEVIER SCIENCE INC

    DOI: 10.1016/j.cbpa.2011.01.020  

    ISSN:1095-6433

    eISSN:1531-4332

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    Meat-type chickens show high feed efficiency and have a very rapid growth rate compared with laying-type chickens. To clarify whether the type-specific difference in feed conversion efficiency is involved in mitochondrial bioenergetics, modular kinetic analysis was applied to oxidative phosphorylation in skeletal muscle mitochondria of both type chickens. Mitochondria from skeletal muscle of meat-type chickens showed greater substrate oxidation and phosphorylating activities, and less proton leak than those of the laying-type, resulting in a higher efficiency of oxidative phosphorylation. Gene expression and protein content of uncoupling protein (avUCP) but not adenine nucleotide translocase (avANT) gene expression were lower in skeletal muscle mitochondria of meat-type chickens than the laying-type. The current results regarding a higher efficiency of oxidative phosphorylation and UCP content may partially support the high feed efficiency of meat-type chickens. (C) 2011 Elsevier Inc. All rights reserved.

  45. Time course of ROS production in skeletal muscle mitochondria from chronic heat-exposed broiler chicken 査読有り

    M. A. K. Azad, M. Kikusato, S. Sudo, T. Amo, M. Toyomizu

    Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiolog 157 (3) 266-271 2010年11月

    出版者・発行元:ELSEVIER SCIENCE INC

    DOI: 10.1016/j.cbpa.2010.07.011  

    ISSN:1095-6433

    詳細を見る 詳細を閉じる

    This study was designed to elucidate physiological changes of skeletal muscle mitochondria from broiler chickens (Gallus gallus) during chronic heat exposure. Chickens (19-day-old) were exposed to either constant heat stress (34 degrees C) or kept at control temperature (24 degrees C) for 14 days. Mitochondrial ROS production for control group showed little changes during the experimental periods, whereas that for the heat-stressed group was increased after 3, 5 and 9 days of heat exposure and returned to original levels at day 14. Mitochondrial membrane potential in state 4 for heat-stressed birds was higher than those of control birds after 3 and 5 days, but was not at day 14. Mitochondrial oxygen consumption rate in state 3 was increased after 3 and 5 days, and also returned to original levels by day 14. These results suggest that chronic heat stress induces increased ROS production in skeletal muscle mitochondria, probably via elevation of the membrane potential in state 4, resulting from enhanced oxygen consumption in the initial stage of heat exposure. These physiological changes were no longer observed at day 14, possibly because the animals had acclimatized to environmental heat stress. (C) 2010 Elsevier Inc. All rights reserved.

  46. Effect of chronic heat stress on performance and oxidative damage in different strains of chickens 査読有り

    Kalam M. A. Azad, Motoi Kikusato, Azharul M. Hoque, Masaaki Toyomizu

    The Journal of Poultry Science 47 (4) 333-337 2010年10月

    出版者・発行元:JAPAN POULTRY SCIENCE ASSOC

    DOI: 10.2141/jpsa.010025  

    ISSN:1346-7395

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    Heat stress (HS) causes poor growth, immunosupression and high mortality, through physiological dysfunction. We have reported that 'acute' HS increases mitochondrial reactive oxygen species (ROS) and oxidative damage in the skeletal muscle of broiler chickens (Cobb), but that this is not for the case for males of the laying-type white leghorn (WLH) strain of chickens (Mujahid etal., 2005b). We have now studied the effects of 'chronic' HS on performance and oxidative damage to skeletal muscle in different strains of chickens. Meat-type male chickens (Ross and Cobb) and male laying-type chickens (WLH) of 14 d of age were arranged according to a factorial design (3 strains X 4 conditions): control (24 C), cyclic (32-24 -32 degrees C: 32 degrees C for 8 h/d, 32-24-32 HS), constant (32 and 34 degrees C, 32 HS and 34 HS, respectively) for 14 d. Feed consumption and body weight gain were measured. Thiobarbituric acid reactive substances (TBARS) content in skeletal muscle was measured colorimetrically with BHT (butylated hydroxytoluene) and expressed as malondialdehyde (MDA) equivalent. Body weight gain and feed consumption for the 3 strains (Ross, Cobb and WLH) were decreased by 32 HS and 34 HS, but not by 32-24 -32 HS. The decrease in weight gain of WLH chickens for the 32 HS and 34 HS conditions was smaller than for broiler chickens (Ross and Cobb). The 32 HS and 34 HS groups showed slightly enhanced MDA levels (P&lt;0.05) in the skeletal muscle of all 3 strains, but not for the 32-24-32 HS group. The results suggest that depression in growth performance due to chronic heat stress is more evident in meat-type chickens (Ross and Cobb) than male laying-type chickens (WLH), and that reduction in the performance are associated with enhancement of oxidative damage to skeletal muscle.

  47. Application of modular kinetic analysis to mitochondrial oxidative phosphorylation in skeletal muscle of birds exposed to acute heat stress 査読有り

    Motoi Kikusato, A. Jon J. Ramsey, Taku Amo, Masaaki Toyomizu

    FEBS Letters 584 (14) 3143-3148 2010年7月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/j.febslet.2010.05.057  

    ISSN:0014-5793

    詳細を見る 詳細を閉じる

    We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (Delta Psi). To clarify the mechanism underlying the elevation of Delta Psi, modular kinetic analysis was applied to oxidative phosphorylation in skeletal muscle mitochondria of heat-stressed birds (34 degrees C for 12 h). In the birds exposed to heat stress, &apos;substrate oxidation&apos; (a Delta Psi-producer) was increased compared to control (24 degrees C) birds, although there was little difference in &apos;proton leak&apos; (a Delta Psi-consumer), suggesting that an elevation in the Delta Psi at state 4 may be due to enhanced substrate oxidation. It thus appears that enhanced substrate oxidation plays a crucial role in the overproduction of ROS for heat-stressed birds, probably via elevated Delta Psi. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

  48. Lactate and adenosine triphosphate in the extender enhance the cryosurvival of rat epididymal sperm 査読有り

    Hideaki Yamashiro, Masaaki Toyomizu, Motoi Kikusato, Natsuki Toyama, Satoshi Sugimura, Yumi Hoshino, Hiroyuki Abe, Stefan Moisyadi, Eimei Sato

    The Journal of the American Association for Laboratory Animal Science 49 (2) 160-166 2010年3月

    出版者・発行元:AMER ASSOC LABORATORY ANIMAL SCIENCE

    ISSN:1559-6109

    詳細を見る 詳細を閉じる

    We evaluated the cryosurvival of rat epididymal sperm preserved in raffinose-modified Krebs-Ringer bicarbonate-egg yolk extender supplemented with various energy-yielding substrates (glucose, pyruvate, lactate, and ATP) and assessed the effect on sperm oxygen consumption. The incubation of sperm at 37 degrees C for 10 min in lactate-free extender decreased sperm motility and oxygen consumption before and after thawing compared with those of sperm in glucose- and pyruvate-free mediums. We then focused on the effect of supplementing the extender with lactate (0, 10.79, 21.58, 32.37, and 43.16 mM) and found that sperm frozen and thawed in extender supplemented with 32.37 mM lactate exhibited the highest motility. When we supplemented extender containing 32.37 mM lactate with ATP (0, 0.92,1.85, 3.70, and 5.55 mM), sperm frozen and thawed in the extender supplemented with 1.85 mM ATP exhibited considerably higher motility and viability than those of sperm frozen and thawed in ATP-free extender. These results provide the first evidence that supplementation of the raffinose-modified Krebs-Ringer bicarbonate-egg yolk extender with 32.37 mM lactate and 1.85 mM ATP increases of number of motile sperm before freezing and enhances the cryosurvival of rat sperm. These supplements to the extender may enhance sperm cryosurvival by improving the metabolic capacity of sperm before freezing.

  49. Metabolic characteristics and oxidative damage to skeletal muscle in broiler chickens exposed to chronic heat stress 査読有り

    M.A.K. Azad, M. Kikusato, T. Maekawa, H. Shirakawa, M. Toyomizu

    Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 155 (3) 401-406 2010年3月

    出版者・発行元:Elsevier BV

    DOI: 10.1016/j.cbpa.2009.12.011  

    ISSN:1095-6433

    eISSN:1531-4332

  50. Mitochondrial energetics and ROS production in chicken skeletal muscle: Application of modular kinetic analysis 招待有り 査読有り

    Toyomizu, M, Kikusato, M, Amo, T, Azad, M.A.K.a

    EAAP Sci. Ser. 127 (1) 39-46 2010年

  51. Mechanistic analysis of mitochondrial ROS production in chicken skeletal muscle under acute heat stress conditions 査読有り

    Kikusato, M, Toyomizu, M

    EAAP Sci. Ser. 127 (1) 69-70 2010年

  52. Up-regulation or activation of avian UCP attenuates mitochondrial ROS production and oxidative damage in broiler chickens exposed to acute heat stress 査読有り

    A. Mujahid, Y. Akiba, M. Kikusato, M. Toyomizu

    ENERGY AND PROTEIN METABOLISM AND NUTRITION (124) 55-56 2007年

    出版者・発行元:WAGENINGEN ACADEMIC PUBLISHERS

    ISSN:0071-2477

︎全件表示 ︎最初の5件までを表示

MISC 44

  1. 焼酎粕ならびにブトキシブチルアルコール給与による肉用鶏の骨格筋タンパク質分解抑制機構の解明

    神園巴美, 神園巴美, 喜久里基, 林國興, 豊水正昭

    栄養生理研究会報 64 (1/2) 2020年

    ISSN:0286-4754

  2. 暑熱感作鶏の骨格筋タンパク質分解に対するグルタミン・グルタミン酸同時給与の抑制効果

    古川恭平, 喜久里基, WU Guoyao, 豊水正昭

    日本家禽学会誌 56 2019年

    ISSN:0029-0254

  3. 急性暑熱感作時における肉用鶏の小胞体ストレス応答の解析

    高梨涼, 徳武優佳子, 徳武優佳子, 古川恭平, 谷高優太, 喜久里基, 喜久里基, 豊水正昭, 豊水正昭

    日本畜産学会大会講演要旨 125th 2019年

    ISSN:1342-4688

  4. 暑熱環境下のニワトリにおける液性免疫機能低下機序の解明

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    日本畜産学会大会講演要旨 125th 2019年

    ISSN:1342-4688

  5. 暑熱環境下における肉用鶏の骨格筋タンパク質分解誘導因子の探索とアミノ酸による制御

    古川 恭平, 喜久里 基, 豊水 正昭

    栄養生理研究会報 = Reviews on Animal Nutrition and Metabolism 63 (1) 43-53 2019年

    出版者・発行元:家畜栄養生理研究会

    ISSN:0286-4754

  6. 肉用鶏へのCNSLならびに加熱CNSL給与時におけるその動態と腸内細菌叢の解析

    永津健太郎, AZAD Md. Abul Kalam, 南都文香, 古川恭平, 平川良太, 喜久里基, 陶山佳久, 森田英利, 嶋田武志, 豊水正昭

    日本家禽学会誌 55 18 2018年3月30日

    ISSN:0029-0254

  7. 暑熱環境が肉用鶏のリンパ組織に及ぼす影響

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    日本畜産学会大会講演要旨 124th 2018年

    ISSN:1342-4688

  8. 鶏における暑熱感作時の骨格筋タンパク質分解亢進の分子メカニズムの解明

    古川恭平, 喜久里基, 豊水正昭

    日本家禽学会誌 55 2018年

    ISSN:0029-0254

  9. 暑熱条件下で抗原接種した鶏の液性免疫応答が遅延する原因解明

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    日本家禽学会誌 55 2018年

    ISSN:0029-0254

  10. 飼料用米給与は暑熱感作時の肉用鶏の生産性に影響するか

    近藤あずさ, 南都文香, 南都文香, 喜久里基, 豊水正昭

    日本家禽学会誌 54 20 2017年3月30日

    ISSN:0029-0254

  11. 寒冷曝露および甲状腺ホルモン給与はニワトリ脂肪組織のベージュ化を誘導するか

    五月女里梨, 喜久里基, 南都文香, 平澤明, 豊水正昭

    日本畜産学会大会講演要旨 122nd 170 2017年3月28日

    ISSN:1342-4688

  12. 黒麹給与が肉用鶏の腸管形態および酸化ストレスに及ぼす影響

    神園巴美, 南都文香, 日置久美子, 山元正博, 喜久里基, 豊水正昭

    日本畜産学会大会講演要旨 122nd 128 2017年3月28日

    ISSN:1342-4688

  13. 熱ストレスを負荷したニワトリにおける解糖減少は免疫応答と関連している(Decreased Glycolysis Relates to Immune Response in Heat-Stressed Chicken)

    Nurjanah Siti, 野地 智法, 南都 文香, 古川 恭平, 阿部 汐里, 福田 栞, 喜久里 基, 豊水 正昭

    日本畜産学会大会講演要旨集 122回 127-127 2017年3月

    出版者・発行元:(公社)日本畜産学会

    ISSN:1342-4688

  14. 生理的濃度のコルチコステロンはニワトリ高温培養筋細胞のタンパク質分解を増強しない

    古川恭平, 喜久里基, 豊水正昭

    日本畜産学会大会講演要旨 122nd 2017年

    ISSN:1342-4688

  15. 飼料用米給与時における鶏の暑熱感作応答

    南都文香, 近藤あずさ, 喜久里基, 松木悠, 陶山佳久, 森田英利, 豊水正昭

    日本畜産学会大会講演要旨 121st 143 2016年3月27日

    ISSN:1342-4688

  16. エネルギー源の異なる高代謝エネルギー(ME)飼料が肉用鶏の飼養成績および肉質におよぼす影響

    近藤あずさ, 南都文香, 喜久里基, 鈴木和明, 豊水正昭

    日本家禽学会誌大会号 52 17 2015年9月10日

    ISSN:0029-0254

  17. 籾米飼料へのトレハロース添加は慢性暑熱曝露鶏の成長と盲腸内細菌叢に影響をおよぼす

    南都文香, 喜久里基, 松木悠, 陶山佳久, 向井和久, 豊水正昭

    日本家禽学会誌大会号 52 23 2015年3月10日

    ISSN:0029-0254

  18. トレハロース給与が幼雛期の肉用鶏の飼養成績・炎症応答におよぼす影響

    喜久里基, 南都文香, 向井和久, 豊水正昭

    日本家禽学会誌大会号 52 23 2015年3月10日

    ISSN:0029-0254

  19. 高温処理時の鶏骨格筋細胞におけるミトコンドリア活性酸素種(ROS)がタンパク質分解に及ぼす影響

    古川恭平, 喜久里基, 神園巴美, 吉田隼巳, 豊水正昭

    日本畜産学会大会講演要旨 119th 2015年

    ISSN:1342-4688

  20. ニワトリ筋細胞におけるオレウロペインのPGC-1α発現誘導作用がミトコンドリア生合成・活性酸素産生におよぼす影響

    室井ひかる, 喜久里基, 上部雄一郎, 古川恭平, 豊水正昭

    日本畜産学会大会講演要旨 120th 2015年

    ISSN:1342-4688

  21. 慢性暑熱曝露時のタンパク質分解関連遺伝子発現亢進におけるミトコンドリア活性酸素種(ROS)の関与

    古川恭平, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会誌 52 2015年

    ISSN:0029-0254

  22. Mitochondrial Function and Poultry Production

    Masaaki TOYOMIZU, Motoi KIKUSATO

    The 10th Asian Pacific Poultry Conference 19-23 2014年10月

  23. 暑熱曝露にともなう鶏腸管の形態損傷ならびに内毒素の血中流入は飼料摂取量低下に起因しない

    南都文香, 喜久里基, 大和田修一, 豊水正昭

    日本家禽学会誌大会号 51 25 2014年9月10日

    ISSN:0029-0254

  24. コエンザイムQ<sub>10</sub>(CoQ<sub>10</sub>)を給与した暑熱感作鶏の肝臓脂質過酸化抑制作用の解明:α‐トコフェロールとアスコルビン酸との相互作用

    三上由紀子, 喜久里基, 南都文香, 伊藤千晶, 豊水正昭

    日本家禽学会誌大会号 51 17 2014年9月10日

    ISSN:0029-0254

  25. 肉用鶏における飼料由来の放射性および非放射性セシウムの体内動態

    土井雄太, 和田道治, 木村和彦, 菅野均志, 白川仁, 喜久里基, 後藤知子, 南都文香, 豊水正昭

    日本畜産学会大会講演要旨 118th 107 2014年3月27日

    ISSN:1342-4688

  26. 高脂肪含有籾米飼料が肉用鶏の発育に及ぼす影響

    伊藤千晶, 南都文香, 神園巴美, 喜久里基, 豊水正昭

    日本畜産学会大会講演要旨 118th 107 2014年3月27日

    ISSN:1342-4688

  27. 慢性暑熱環境下における飼料用籾米飼料給与が肉用鶏腸管の形態,機能および免疫応答に与える影響

    南都文香, 喜久里基, 大和田修一, 高橋和昭, 豊水正昭

    日本家禽学会誌大会号 51 24 2014年3月10日

    ISSN:0029-0254

  28. 枯草菌添加飼料が鶏の増体量・腸管形態・腸内細菌叢に及ぼす影響

    平畝真奈美, 喜久里基, 南都文香, 芦田延久, 今林寛和, 豊水正昭

    日本家禽学会誌大会号 51 18 2014年3月10日

    ISSN:0029-0254

  29. 電解還元水給与による慢性暑熱曝露鶏の酸化ストレス緩和機構の解明ならびに炎症応答抑制効果の検証

    松野良平, 喜久里基, 古川恭平, 大津晴彦, 高橋和昭, 豊水正昭

    日本家禽学会誌 51 2014年

    ISSN:0029-0254

  30. 培養骨格筋細胞を用いた家禽の暑熱ストレス発生機序の解明 -活性酸素の過剰産生が体タンパク質分解亢進に及ぼす影響̶-

    喜久里基, 吉田隼巳, 古川恭平, 神園巴美, 豊水正昭

    家畜栄養生理研究会報 54 (57) 66 2014年

  31. 培養骨格筋細胞を用いた家禽の暑熱ストレス発生機序の解明 : 活性酸素の過剰産生が体タンパク質分解亢進に及ぼす影響

    喜久里 基, 吉田 隼巳, 古川 恭平, 神園 巴美, 豊水 正昭

    栄養生理研究会報 = Proceedings of Japanese Society for Animal Nutrition and Metabolism 58 (2) 53-63 2014年

    出版者・発行元:家畜栄養生理研究会

    ISSN:0286-4754

    詳細を見る 詳細を閉じる

    暑熱環境下における肉用鶏の生産性低下は古くからの問題であり、近年激化の様相を示す夏季の暑熱によって肉用鶏産業が大きな損失を被ることは想像に難くない。それゆえに、暑熱時に発生する肉用鶏生産の量的・質的な低下を改善する生産技術体系の確立は重要な課題である。暑熱ストレスによる生産性の低下を真に改善するためには、肉用鶏が暑熱下でどのような生理反応・生体応答を示しているのか、その全貌を明らかにすることが肝要である。これまでの研究において、暑熱環境下では熱放散促進のための浅速呼吸にともなう呼吸性アルカローシスの発症、甲状腺ホルモンの低下・コルチコステロンの増加にともなう体タンパク質分解亢進、腸管微絨毛の損傷・免疫応答の変化ならびにミトコンドリア活性酸素(reactive oxygen species、ROS)の過剰産生による酸化ストレスが生じることが明らかにされている。このため、呼吸性アルカローシス発症を抑制するための重曹(重炭酸ナトリウム)の給与や、酸化ストレス発生抑制のためのビタミンCやビタミンE、ポリフェノール類、電解還元水といった抗酸化物質の給与による暑熱ストレス緩和の有効性が調べられてきている。しかしながら、これらはいずれも暑熱時の生産性を真に改善するまでには至っておらず、暑熱時の生体応答の一部改善を目的とした対策としては十分ではない。

  32. 高脂肪飼料摂取マウスにおける末梢セロトニンの褐色脂肪組織ミトコンドリア活性化作用

    齋藤遼, 渡邉一史, 中野辰也, 住吉敬介, 長澤裕哉, 喜久里基, 渡邊康一, 麻生久

    日本畜産学会大会講演要旨 116th 96-96 2013年3月28日

    出版者・発行元:(公社)日本畜産学会

    ISSN:1342-4688

  33. 肉用鶏が放射性セシウムを摂取した時の体内蓄積について

    豊水正昭, 土井雄太, 南都文香, 喜久里基, 木島裕美, 白川仁, 和田道治

    日本畜産学会大会講演要旨 116th 128 2013年3月28日

    ISSN:1342-4688

  34. 慢性暑熱環境下におけるMEの異なる飼料用籾米飼料給与が肉用鶏の生産性に与える影響

    南都文香, 伊藤千晶, 喜久里基, 豊水正昭

    日本家禽学会誌大会号 50 15 2013年3月8日

    ISSN:0029-0254

  35. 末梢セロトニンの褐色脂肪細胞活性化作用

    齋藤遼, 渡邉一史, 中野辰也, 住吉敬介, 喜久里基, 長澤裕哉, 寺田俊介, 岡田夏実, 渡邊康一, 麻生久

    日本獣医学会学術集会講演要旨集 154th 170-170 2012年8月31日

    出版者・発行元:(公社)日本獣医学会

    ISSN:1347-8621

  36. 非放射性セシウム添加飼料を肉用鶏に給与し続けた時の体内蓄積応答

    土井雄太, 菅野均志, 木村和彦, 後藤知子, 白川仁, 喜久里基, 南都文香, 豊水正昭

    日本家禽学会誌大会号 49 15 2012年8月20日

    ISSN:0029-0254

  37. 植物性油脂を多く含む全粒籾米飼料給与により肉用鶏の成長は低下する

    伊藤千晶, 南都文香, 神園巴美, 松枝朝子, 喜久里基, 豊水正昭

    日本家禽学会誌大会号 49 14 2012年3月10日

    ISSN:0029-0254

  38. 飼料用米給与が肉用鶏の生産性におよぼす影響

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会大会講演要旨 114th 85 2011年8月26日

    ISSN:1342-4688

  39. 飼料用米給与が肉用鶏の生産性におよぼす影響

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会大会講演要旨 114th 120 2011年8月26日

    ISSN:1342-4688

  40. 肉用鶏生産における急性および慢性暑熱ストレス応答に対する飼料用籾米の影響

    南都文香, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会誌大会号 48 12 2011年8月10日

    ISSN:0029-0254

  41. 肉用鶏生産における飼料用米利用の有効性

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本家禽学会誌大会号 48 18 2011年3月5日

    ISSN:0029-0254

  42. 急性暑熱にともなう鶏骨格筋におけるROS過剰産生機構の解明 -バイオエナジェティクスの観点からのアプローチ-

    喜久里基, 豊水正昭

    家畜栄養生理研究会報 54 57-66 2010年5月

  43. Exogenous lactate and adenosine triphosphate in the extender regulated motility, oxidative activity and enhanced cryosurvival of rat epididymal sperm

    Yamashiro,H, Toyomizu,M, Kikusato,M, Toyama,N, Sugimura, S, Hoshino,Y, Abe,H, Moisyadi,S, Sato,E

    J. Am. Assoc. Lab. Anim. Sci. 49 (2) 1-7 2010年

  44. 還元水飲水が暑熱時の鶏肉生産性および品質に及ぼす影響

    KALAM Azad Abul, 今成麻衣, 喜久里基, 藤村忍, 樺山繁, 豊水正昭

    日本家禽学会誌 45 2008年

    ISSN:0029-0254

︎全件表示 ︎最初の5件までを表示

書籍等出版物 2

  1. 動物の栄養第2版

    唐澤豊, 菅原邦夫, 本人含む

    文英堂出版 2016年2月20日

    ISBN: 9784830041303

  2. 日本飼養標準 家禽(2011年度版)

    阿部啓之, 池谷守司, 本人含む

    中央畜産会出版 2012年3月14日

講演・口頭発表等 105

  1. 飼料中トウモロコシ粒度が肉用鶏の成長・消化管におよぼす影響

    松橋花菜子, 渡邊康一, 豊水正昭, 喜久里基

    日本家禽学会2018年春季大会 2018年3月30日

  2. 肉用鶏へのアナカルド酸給与時におけるその動態と腸内細菌叢の解析

    永津健太郎, Md. Abul Kalam Azad, 南都文香, 古川恭平, 平川良太, 喜久里基, 豊水正昭

    日本家禽学会2018年春季大会 2018年3月30日

  3. Effects of oleuropein on lipopolysaccharide-induced hepatic injury in mice

    Ayaka Otani, Hitoshi Shirakawa, Tomonori Nochi, Masaaki Toyomizu, Motoi Kikusato

    日本畜産学会第124回大会 2018年3月28日

  4. 暑熱環境が肉用鶏のリンパ組織に及ぼす影響

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    日本畜産学会第124回大会 2018年3月28日

  5. Differences in mitochondrial fatty acid utilization and respiratory complex characteristics between meat-type and laying-type chickens 国際会議

    Hakamata Y, Amo T, Toyomizu M, Kikusato, M

    11th Asia Pacific Poultry Conference 2018年3月25日

  6. Rice diet containing high fat and rice hull affects the growth performance or heat-exposed broiler chickens 国際会議

    Nanto F, Kikusato M, Owada S, Toyomizu M

    5th EAAP International Symposium on Energy and Protein Metabolism and Nutrition 2017年9月12日

  7. 5th EAAP International Symposium on Energy and Protein Metabolism and Nutrition 国際会議

    Kamizono T, Kikusato M, Hayashi K, Toyomizu M

    EAAP International Symposium on Energy and Protein Metabolism and Nutrition 2017年9月12日

  8. Roles of corticosterone and superoxide in the ubiquitin proteasome system in heat-stressed chickens 国際会議

    Furukawa K, Kikusato M, Toyomizu M

    5th EAAP International Symposium on Energy and Protein Metabolism and Nutrition 2017年9月12日

  9. Comparisons of ROS homeostasis in avian skeletal muscle with different myofiber compositions 国際会議

    Kikusato M, Toyomizu M

    5th EAAP International Symposium on Energy and Protein Metabolism and Nutrition 2017年9月12日

  10. Time-course of changes in muscle antioxidant peptide concentrations in heat-stressed broiler chickens 国際会議

    Furukawa K, Waki N, Kikusato M, Ashinara A, Ishida A, Nakashima K, Toyomizu M

    17th Animal Science Congress of Asian Australasian Animal Production 2017年8月22日

  11. Effects of bacillary probiotic (Bacillus subtilis C-3102) in spore form on the growth performance and intestinal immunity of heat-stressed broiler

    Kikusato M, Nanto F, Ishikawa T, Ashida N, Imabayashi T, Toyomizu M

    17th Animal Science Congress of Asian Australasian Animal Production 2017年8月22日

  12. Defining the mitochondrial histochemical characteristics of avian skeletal muscles with different muscle fiber type compositions

    Hakamata Y, Kikusato M, Watanabe K, Amo T, Toyomizu M

    17th Animal Science Congress of Asian Australasian Animal Production 2017年8月22日

  13. Possible roles of corticosterone in proteolysis, antioxidant system, and glycolytic genes in avian skeletal muscle cells under heat stress conditions 国際会議

    Furukawa K, Kikusato M, Toyomizu M

    Tohoku Forum for Creativity Thematic Program 2017 2017年8月7日

  14. Effect of chronic heat stress on splenic immunologic function and amino acid concentration in broiler chickens 国際会議

    Hirakawa R, Nurjanah S, Nochi T, Furukawa K, Kikusato M, Toyomizu M

    Tohoku Forum for Creativity Thematic Program 2017 2017年8月7日

  15. Effects of oleuropein on lipopolysaccharide-induced liver injury 国際会議

    Otani A, Shirakawa H, Toyomizu M, Kikusato M

    Tohoku Forum for Creativity Thematic Program 2017 2017年8月7日

  16. Association of electron transport chain supercomplexes with reactive oxygen species generation in avian skeletal muscles with different muscle fiber compositions 国際会議

    Hakamata Y, Watanabe K, Amo T, Toyomizu M, Kikusato M

    Tohoku Forum for Creativity Thematic Program 2017 2017年8月7日

  17. Bioavailability of polyphenol: How do polyphenols act inside the body? 国際会議

    Tohoku Forum for Creativity Thematic Program 2017 2017年8月7日

  18. Oleuropein suppresses oxidative damage in avian skeletal muscle, possibly via SIRT1 up-regulation 国際会議

    Kikusato M, Shimao R, Muroi H, Toyomizu M

    21st European Symposium on Poultry Nutrition 2017年5月8日

  19. オリーブ特有フェノール化合物オレウロペインがニワトリ骨格筋の活性酸素産生におよぼす影響

    室井ひかる, 嶋尾里紗, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会2017年春季大会 2017年3月30日

  20. 飼料用米給与は暑熱感作時の肉用鶏の生産性に影響するか

    近藤あずさ, 南都文香, 喜久里基, 豊水正昭

    日本家禽学会2017年春季大会 2017年3月30日

  21. 酸素消費量高低選抜マウスにおける褐色脂肪組織のミトコンドリア呼吸活性

    紅玉, 田仲誠一郎, 喜久里基, 豊水正昭, 佐藤正寛, 鈴木啓一

    日本畜産学会第122回大会 2017年3月28日

  22. 生理的濃度のコルチコステロンはニワトリ高温培養筋細胞のタンパク質分解を増強しない

    古川恭平, 喜久里基, 豊水正昭

    日本畜産学会第122回大会 2017年3月28日

  23. 寒冷曝露および甲状腺ホルモン給与はニワトリ脂肪組織のベージュ化を誘導するか

    五月女里梨, 喜久里基, 南都文香, 平澤明, 豊水正昭

    日本畜産学会第122回大会 2017年3月28日

  24. Decreased Glycolysis Relates to Immune Response in Heat-Stressed Chicken

    Nurjanah Siti, 野地智法, 南都文香, 古川恭平, 阿部汐里, 福田栞, 喜久里基, 豊水正昭

    日本畜産学会第122回大会 2017年3月28日

  25. 黒麹給与が肉用鶏の腸管形態および酸化ストレスに及ぼす影響

    神園巴美, 南都文香, 日置久美子, 山元正博, 喜久里基, 豊水正昭

    日本畜産学会第122回大会 2017年3月28日

  26. 家禽⽣産におけるトレハロースの利⽤

    第20 回トレハロースシンポジウム 2016年11月17日

  27. 暑熱負荷鶏の浅胸筋および腓腹筋におけるミトコンドリア活性酸素産生制御の比較解析

    喜久里基, 豊水正昭

    日本家禽学会2016年春季大会 2016年3月30日

  28. 筋線維型が異なるニワトリ骨格筋におけるミトコンドリア呼吸活性の差異

    袴田祐基, 喜久里基, 渡邊康一, 豊水正昭

    日本畜産学会第121回大会 2016年3月27日

  29. 段階的暑熱環境下における肉用鶏の酸化ストレスに対するコエンザイムQ10(CoQ10)の抑制作用

    三上由紀子, 喜久里基, 豊水正昭

    日本畜産学会第121回大会 2016年3月27日

  30. 飼料用米給与時における鶏の暑熱感作応答

    南都文香, 近藤あずさ, 喜久里基, 松木悠, 陶山佳久, 森田英利, 豊水正昭

    日本畜産学会第121回大会 2016年3月27日

  31. エネルギー源の異なる高代謝エネルギー(ME)飼料が肉用鶏の飼養成績および肉質におよぼす影響

    近藤あずさ, 南都文香, 喜久里基, 豊水正昭

    日本家禽学会2015年秋季大会 2015年9月10日

  32. ニワトリ筋細胞におけるオレウロペインのPGC-1α発現誘導作用がミトコンドリア生合成・活性酸素産生におよぼす影響

    室井ひかる, 喜久里基, 上部雄一郎, 古川恭平, 豊水正昭

    日本畜産学会第120回大会 2015年9月10日

  33. 鶏における慢性暑熱ストレスに対する緩和飼料資材の探索:電解還元水とセレン酵母の給与効果

    松野良平, 喜久里基, 豊水正昭

    日本家禽学会2015年春季大会 2015年3月30日

  34. 慢性暑熱曝露時のタンパク質分解関連遺伝子発現亢進におけるミトコンドリア活性酸素種(ROS)の関与

    古川恭平, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会2015年春季大会 2015年3月30日

  35. 籾米飼料へのトレハロース添加は慢性暑熱曝露鶏の成長と盲腸内細菌叢に影響をおよぼす

    南都文香, 喜久里基, 松木悠, 陶山佳久, 向井和久, 豊水正昭

    日本家禽学会2015年春季大会 2015年3月30日

  36. トレハロース給与が幼雛期の肉用鶏の飼養成績・炎症応答におよぼす影響

    喜久里基, 南都文香, 向井和久, 豊水正昭

    日本家禽学会2015年春季大会 2015年3月30日

  37. 寒冷曝露下の鶏における骨格筋FNDC5/Irisin発現と脂肪組織avUCPの連関

    栗谷川あんな, 喜久里基, 白川仁, 天羽拓, 豊水正昭

    日本畜産学会第119回大会 2015年3月28日

  38. 高温処理時の鶏骨格筋細胞におけるミトコンドリア活性酸素種(ROS)がタンパク質分解に及ぼす影響

    古川恭平, 喜久里基, 神園巴美, 吉田隼巳, 豊水正昭

    日本畜産学会第119回大会 2015年3月28日

  39. コエンザイムQ10(CoQ10)を給与した暑熱感作鶏の肝臓脂質過酸化抑制作用の解明:α-トコフェロールとアスコルビン酸との相互作用

    三上由紀子, 喜久里基, 南都文香, 伊藤千晶, 豊水正昭

    日本家禽学会2014年春秋季大会 2014年9月27日

  40. 暑熱曝露にともなう鶏腸管の形態損傷ならびに内毒素の血中流入は飼料摂取量低下に起因しない

    南都文香, 喜久里基, 大和田修一, 豊水正昭

    日本家禽学会2014年春秋季大会 2014年9月27日

  41. Oleuropein, one of polyphenols in olive oil, attenuates heat stress-induced overproduction of mitochondrial reactive oxygen species in skeletal muscle of birds 国際会議

    Uwabe, Y, Toyomizu, M

    EPC2014 2014年6月23日

  42. 枯草菌添加飼料が鶏の増体量・腸管形態・腸内細菌叢に及ぼす影響

    平畝真奈美, 南都文香, 芦田延久, 今林寛和, 豊水正昭

    日本家禽学会2014年春季大会 2014年3月29日

  43. ブトキシブチルアルコールはブロイラーの骨格筋タンパク質代謝に影響して成長を促進する

    神園巴美, 林國興, 豊水正昭

    日本家禽学会2014年春季大会 2014年3月29日

  44. オリーブオイル中の鶏骨格筋脱共役タンパク質(UCP)発現誘導因子の探索

    上部雄一郎, 豊水正昭

    日本家禽学会2014年春季大会 2014年3月29日

  45. 電解還元水給与による慢性暑熱曝露鶏の酸化ストレス緩和機構の解明ならびに炎症応答抑制効果の検証

    松野良平, 喜久里基, 古川恭平, 大津晴彦, 高橋和昭, 豊水正昭

    日本家禽学会2014年春季大会 2014年3月29日

  46. 慢性暑熱環境下における飼料用籾米飼料給与が肉用鶏腸管の形態、機能および免疫応答に与える影響

    南都文香, 大和田修一, 高橋和昭, 豊水正昭

    日本家禽学会2014年春季大会 2014年3月29日

  47. 高脂肪含有籾米飼料が肉用鶏の発育に及ぼす影響

    伊藤千晶, 南都文香, 神園巴美, 豊水正昭

    日本畜産学会第118回大会 2014年3月27日

  48. 肉用鶏における飼料由来の放射性および非放射性セシウムの体内動態

    土井雄太, 和田道治, 木村和彦, 菅野均志, 白川仁, 後藤知子, 南都文香, 豊水正昭

    日本畜産学会第118回大会 2014年3月27日

  49. ピロロキノリンキノン(PQQ)の添加が単離鶏ミトコンドリアの呼吸機能に及ぼす影響

    田邉拓也, 天羽拓, 豊水正昭

    日本畜産学会第118回大会 2014年3月27日

  50. Electrolyzed reduced water ameliorates heat stress-induced oxidative damage to liver, possibly via a deacetylation of superoxide dismutase 2 国際会議

    Matsuno, R, Furukawa, K, Matsueda, T, Toyomizu, M

    SFRRI2014 2014年3月23日

  51. Mitochondrial ROS production in skeletal muscle is exquisitely sensitive to the membrane potential regardless of the myofiber type compositions 国際会議

    Toyomizu, M

    Mitochondria 2013 2013年11月6日

  52. Does heat-induced ROS regulate protein content in chick muscle cells? 国際会議

    Yoshida, H, Souma, K, Toyomizu, M

    ISEP2013 2013年9月9日

  53. Heat stress-induced overproduction of mitochondrial ROS is down-regulated in laying-type chickens 国際会議

    Toyomizu, M

    ISEP2013 2013年9月9日

  54. Effect of paddy rice diets on performance in chickens under thermoneutral and heat stress conditions 国際会議

    Nanto F, Ito C, Toyomizu, M

    ISEP2013 2013年9月9日

  55. 暑熱ストレス研究の近年の動向

    平成25年度関東甲信越地域鶏試験研究推進協議会 2013年8月8日

  56. 慢性暑熱環境下におけるMEの異なる飼料用籾米飼料給与が肉用鶏の生産性に与える影響

    南都文香, 伊藤千晶, 喜久里基, 豊水正昭

    2013年度日本家禽学会春季大会 2013年3月29日

  57. In vitro暑熱ストレスモデルを用いたROS過剰産生機構の解明

    吉田隼巳, 喜久里基, 相馬邦彦, 豊水正昭

    日本畜産学会第116回大会 2013年3月28日

  58. ピロロキノリンキノン(PQQ)の添加が単離鶏肝臓ミトコンドリアの酸化的リン酸化能に及ぼす影響

    田邉拓也, 喜久里基, 豊水正昭

    日本畜産学会第116回大会 2013年3月28日

  59. 肉用鶏の浅胸筋および腓腹筋におけるミトコンドリアROS産生応答

    喜久里基, 豊水正昭

    日本畜産学会第116回大会 2013年3月28日

  60. Electrolyzed-reduced water alleviates oxidative stress induced by chronic heat exposure 国際会議

    Matsueda, T, Kikusato, M, Azad, M.A.K, Toyomizu, M, raduate School of Agricultural Science, Tohoku Universi

    INSDH2012 2012年10月16日

  61. Oxidative stability of rice-based diets may affect growth performance of broiler chickens 国際会議

    Ito, C, Nanto, F, Kamizono, T, Matsueda, T, Kikusato, M, Toyomizu, M, Graduate School of Agricultural Science, Tohoku Universi

    INSDH2012 2012年10月16日

  62. 非放射性セシウム添加飼料を肉用鶏に給与し続けた時の体内蓄積応答

    土井雄太, 菅野均志, 木村和彦, 後藤知子, 白川仁, 喜久里基, 南都文香, 豊水正昭, 東北大院農, 城大食

    2012年度日本家禽学会秋季大会 2012年9月13日

  63. 産卵鶏は急性暑熱曝露時においても酸化ストレスは亢進しない

    喜久里基, 豊水正昭

    2012年度日本家禽学会秋季大会 2012年9月13日

  64. Effect of dietary C5 Molasses (derived from Empty Fruit Bunches) on growth performance and meat quality in broilers 国際会議

    Kamizono, T, Kikusato, M, Toyomizu, M, Graduate School of Agricultural Science, Tohoku Universi

    XXIV World’s Poultry Congress 2012年8月5日

  65. Effects of whole paddy rice on growth performance and oxidative stress in broiler chickens exposed to acute and chronic heat stress 国際会議

    Nanto, F, Kikusato, M, Kamizono, T, Toyomizu, M, Graduate School of Agricultural Science, Tohoku Universi

    XXIV World’s Poultry Congress 2012年8月5日

  66. Dietary coenzyme Q10 suppresses heat stress-induced overproduction of ROS in skeletal muscle of birds, but not via mitochondrial membrane potential or basal proton leak 国際会議

    Kikusato, M, Nakamura, K, Toyomizu, M, Graduate School of Agricultural Science, Tohoku Universi

    XXIV World’s Poultry Congress 2012年8月5日

  67. Olive oil (refined)-enriched diet upregulates avian uncoupling protein expression and proton leak in the skeletal muscle mitochondria 国際会議

    Kikusato, M, Yuichiro, U, Toyomizu, M, Graduate School of Agricultural Science, Tohoku University

    10th Congress of the International Society for the Study Fatty Acids and Lipids 2012年5月26日

  68. 中山泰史・喜久里基・須藤翔太・松枝朝子・豊水正昭

    日本家禽学会2012年度春季大会 2012年3月30日

  69. 植物性油脂を多く含む全粒籾米飼料給与により肉用鶏の成長は低下する

    伊藤千晶, 南都文香, 神園巴美, 松枝朝子, 喜久里基, 豊水正昭

    日本家禽学会2012年度春季大会 2012年3月30日

  70. 鶏骨格筋細胞を用いた暑熱ストレスモデルの構築

    吉田隼巳, 相馬邦彦, 喜久里基, 豊水正昭

    日本畜産学会第115回大会 2012年3月28日

  71. BCAAs三成分系における鶏骨格筋細胞の増殖および代謝応答

    相馬邦彦, 喜久里基, 佐藤幹, 豊水正昭

    日本畜産学会第115回大会 2012年3月28日

  72. Crucial role of membrane potential in heat-stress induced overproduction of reactive oxygen species in skeletal muscle mitochondria 国際会議

    Kikusato, M, Toyomizu, M

    5th SFRR-Asia/8th ASMRM/11th J-mit International Conference 2011年8月31日

  73. Modular kinetic analysis reveals that meat-type chickens have a higher efficiency of mitochondrial of mitochondrial oxidative phosphorylation than laying-type chickens 国際会議

    Amo, T, Kikusato, M, Kawabata, Y, Azad, MAK, Inui, E, Toyomizu, M

    5th SFRR-Asia/8th ASMRM/11th J-mit International Conference 2011年8月31日

  74. 急性暑熱感作鶏における酸化損傷の組織特異性

    喜久里基, 豊水正昭

    日本畜産学会第114回大会 2011年8月26日

  75. メチオニン欠乏時における鶏骨格筋ミトコンドリアの代謝応答

    須藤翔太, 喜久里基, 豊水正昭

    日本畜産学会第114回大会 2011年8月26日

  76. 飼料用米給与が肉用鶏の生産性におよぼす影響

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会第114回大会 2011年8月26日

  77. 鶏における炎症応答に対するカシューナッツ殻油給与の影響

    鈴木脩悟, 斎藤雄介, 喜久里基, 高橋和昭, 豊水正昭

    日本畜産学会第114回大会 2011年8月26日

  78. 飼料用米給与が肉用鶏の生産性におよぼす影響

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会第114回大会 2011年8月26日

  79. 慢性暑熱感作時のブロイラーにおける電解還元水および水素水による酸化ストレス緩和効果

    松枝朝子, 喜久里基, 須藤翔太, 豊水正昭

    日本家禽学会2011年度秋季大会 2011年8月24日

  80. 肉用鶏生産における急性および慢性暑熱ストレス応答に対する飼料用米籾米の影響

    南都文香, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会2011年度秋季大会 2011年8月24日

  81. 鶏の急性暑熱ストレスに対する脱共役タンパク質UCPの役割

    第21回東北大学大学院農学研究科先端農学セミナー 2011年6月17日

  82. 肉用鶏生産における飼料用米利用の有効性

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本家禽学会2011年度春季大会 2011年3月28日

  83. 急性暑熱時のコハク酸酸化にともなう鶏骨格筋ミトコンドリアのROS産生応答

    喜久里基, 豊水正昭

    日本畜産学会第111回大会 2010年9月28日

  84. 鶏初代培養肝細胞における活性酸素種(ROS)産生に対するアナカルド酸の影響

    鈴木脩悟, 前川喬彦, 喜久里基, 豊水正昭

    日本畜産学会第111回大会 2010年9月28日

  85. 急性暑熱感作鶏におけるエネルギー代謝関連遺伝子発現の網羅的解析

    喜久里基, 白川仁, 豊水正昭

    日本家禽学会2010年度秋季大会 2010年9月14日

  86. 鶏骨格筋初代培養細胞の増殖に対する三成分系分岐鎖アミノ酸による栄養制御

    相馬邦彦, 喜久里基, 佐藤幹, 豊水正昭

    日本家禽学会2010年度秋季大会 2010年9月14日

  87. 肉用鶏のミトコンドリア酸化的リン酸化効率は産卵鶏より良い

    豊水正昭, 喜久里基, 川端雄亮, Azad, MAK, 乾恵理子, 天羽拓

    日本家禽学会2010年度秋季大会 2010年9月14日

  88. Mechanistic analysis of mitochondrial ROS production in chicken skeletal muscle under acute heat stress conditions 国際会議

    Kikusato, M, Toyomizu, M

    3rd EAAP International Symposium of Energy and Protein Metabolism and Nutrition 2010年6月6日

  89. Mitochondrial energetics and ROS production in chicken skeletal muscle: application of modular kinetic analysis 国際会議

    Toyomizu, M, Kikusato, M, Amo, T, Azad, MAK

    3rd EAAP International Symposium of Energy and Protein Metabolism and Nutrition 2010年6月6日

  90. 急性暑熱感作における活性酸素種(ROS)の産生増大はどんなときにおこるか?

    喜久里基, 豊水正昭

    日本家禽学会2010年度春季大会 2010年3月29日

  91. 不飽和度の異なる油脂給与鶏における骨格筋における骨格筋ミトコンドリアのバイオエナジェティクス

    松本祐世, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会第112回大会 2010年3月27日

  92. メチオニン欠乏が鶏骨格筋ミトコンドリア機能に及ぼす影響

    須藤翔太, 喜久里基, 松本祐世, 高橋和昭, 豊水正昭

    日本畜産学会第112回大会 2010年3月27日

  93. Depression in proton leak under heat stress stimulates mitochondrial ROS production regardless of membrane potential 国際会議

    Kikusato, M, Toyomizu, M

    1st International Conference on Lipid Hydroperoxide biology and medicine 2009年11月4日

  94. Time course of ROS production in skeletal muscle mitochondria from chronic heat-exposed broiler chickens 国際会議

    Azad, MAK, Kikusato, M, Matsumoto, Y, Toyomizu, M

    1st International Conference on Lipid Hydroperoxide biology and medicine 2009年11月4日

  95. Anacardic acid attenuates TNFα/IFNγ-stimulated ROS production in chicken hepatocytes, probably via mild uncoupling action 国際会議

    Suzuki, S, Kikusato, M, Maekawa, T, Takahashi, K, Toyomizu, M

    1st International Conference on Lipid Hydroperoxide biology and medicine 2009年11月4日

  96. 急性暑熱曝露にともなう鶏骨格筋ミトコンドリアのプロトリーク応答

    喜久里基, 乾恵理子, 豊水正昭

    日本畜産学会第110回大会 2009年3月27日

  97. 低温環境馴化時における鶏脱共役タンパク質avUCPの発現応答とその機能の解明

    乾恵理子, 喜久里基, 松本祐世, 豊水正昭

    日本畜産学会第110回大会 2009年3月27日

  98. 高温環境下における家禽ミトコンドリアの代謝応答

    東北大学国際高等融合領域研究所 研究討論会 2009年1月28日

  99. 急性暑熱ストレスにともなう鶏骨格筋ミトコンドリアROS過剰産生の代謝解析

    喜久里基, 天羽拓, 豊水正昭

    日本酸化ストレス学会第61回大会 2008年6月19日

  100. Effect of chronic heat stress on performance and oxidative damage in different strains of chicken

    Azad, MAK, Kikusato, M, Mujahid, A, Toyomizu, M

    日本家禽学会2008年度春季大会 2008年3月28日

  101. 還元水飲水が暑熱時の鶏肉生産性および品質に及ぼす影響

    Azad, MAK, 今成麻衣, 喜久里基, 藤村忍, 樺山繁, 豊水正昭

    日本家禽学会2008年度春季大会 2008年3月28日

  102. 急性暑熱曝露下における鶏骨格筋ミトコンドリアの膜電位特性のTop-downアプローチによる解析

    喜久里基, 天羽拓, Ramsey, JJ, 豊水正昭

    日本畜産学会第109回大会 2008年3月27日

  103. Up-regulation or activation of avian UCP attenuates mitochondrial ROS production and oxidation damage in broiler chickens exposed to acute heat stress 国際会議

    Mujahid, A, Akiba, Y, Kikusato, M, Toyomizu, M

    2nd EAAP International Symposium of Energy and Protein Metabolism and Nutrition 2007年9月9日

  104. 急性暑熱(38°C)曝露した肉用鶏の直腸温ならびに血漿・肝臓・骨格筋のMDAに対する絶食とCTCの影響

    喜久里基, 林國興, Mujahid, A, 秋葉征夫, 豊水正昭

    日本畜産学会第107回大会 2007年3月27日

  105. Adrenal hormones play important roles to tolerant acute heat stress in broilers 国際会議

    Kikusato, M, Kato, N, Ohtsuka, A, Hayashi, K

    Poultry Science Association 95th Annual Meeting 2006年6月16日

︎全件表示 ︎最初の5件までを表示

共同研究・競争的資金等の研究課題 12

  1. ニワトリの卵はゆでるとなぜ硫黄臭いのか? 胚発生における硫黄呼吸の実体解明

    喜久里 基, 徳武 優佳子

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    研究種目:Grant-in-Aid for Challenging Research (Exploratory)

    研究機関:Tohoku University

    2021年7月 ~ 2024年3月

  2. 腸管を基軸とした幼若雛の健全育成を支えるニワトリ粘膜免疫学の創成

    野地 智法, イスラム ジャヒドゥル, 佐藤 幹, 喜久里 基, 陶山 佳久, 松尾 歩

    2021年7月9日 ~ 2023年3月31日

    詳細を見る 詳細を閉じる

    ニワトリ(鶏)の免疫学に関する教科書には、骨髄で造血幹細胞から分化したB細胞前駆細胞は、排泄腔近傍に存在するファブリキウス嚢でB細胞へと分化し、その後、脾臓やパイエル板(回腸終末部)、盲腸扁桃(盲腸基部)といった免疫臓器で機能すると記載されている(Avian Immunology 2nd Edition)。一方で、我々は、広大な表面積を有する盲腸にも成熟したB細胞が多数集積する濾胞構造が広範囲に発達していることを見出し、盲腸扁桃やパイエル板とは異なる、腸管に発達する新たな免疫臓器(Cecal patches: CP)の存在を明らかにしてきた。加えて、経時的に実施した形態学的解析を通して、CPは盲腸扁桃と同様に孵化後に形態形成されること、腸管の免疫機能に深く関わるIgA(免疫グロブリンのサブクラスの1つ)を産生する形質細胞へのB細胞からの最終分化が、盲腸扁桃やパイエル板以上に、CPで盛んに行われていることを見出し、既存の教科書には記載されていない、孵化後の鶏の腸管内で発達するCPを中心とした粘膜免疫システムの存在を提唱してきた。 <BR> そこで本研究では、孵化1, 2, 3, 5, 7 週間後の肉用鶏(Ross308系)より、盲腸を採材・固定し、CPの形態形成を組織学的に解析した。また、組織切片をIgM, IgA, IgYをマーカーとした免疫組織化学染色に供することで、抗体産生細胞の局在変化も比較解析した。その結果、CP形成が開始される時期は、盲腸扁桃の発達時期よりも少し遅く、孵化2週齢以降であることが明らかになった。このことは、盲腸扁桃とCPの形態形成を促す刺激は異なっている可能性を強く示唆していた。さらには、CPで濾胞構造が発達する時期は3週齢前後であり、同時期に濾胞内でB細胞からIgA産生細胞への最終分化が起きていることも明らかになった。

  3. 暑熱ニワトリの摂食低下機構の解明:腸管バリア機能破綻を起点とした脳摂食抑制の検証

    喜久里 基, 米山 裕, 小島 創一, 橘 哲也, 村井 篤嗣

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:Tohoku University

    2020年4月 ~ 2023年3月

  4. 暑熱鶏において骨格筋タンパク質代謝破綻をもたらす他臓器間代謝ネットワークの解明 競争的資金

    喜久里 基

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

    研究種目:Grant-in-Aid for Young Scientists (A)

    研究機関:Tohoku University

    2016年4月 ~ 2020年3月

    詳細を見る 詳細を閉じる

    暑熱ニワトリにおいて、筋タンパク質分解を誘導する臓器間代謝ネットワーク機構を明らかにするため、コルチコステロン(CORT)、ミトコンドリア活性酸素産生(mitROS)、腸管バリア機能、炎症応答、アミノ酸動態の連関に着目し研究を行った。その結果、暑熱ニワトリにおける①mitROS産生が筋タンパク質分解の誘導要因の一つであること、②腸管バリア機能破綻にともなうLPS流入がCORT分泌や3メチルヒスチジン濃度上昇に関与、③筋タンパク質由来のアルギニンが暑熱時の炎症反応に影響を与えている可能性が示された。以上より、暑熱時の筋タンパク質分解は全身性炎症の一環で生じることが明らかになった。

  5. 種鶏・種卵へのマイルドな熱感作による肉用鶏への耐暑性付与と遺伝子発現特性の解析

    喜久里 基

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    研究種目:Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    研究機関:Tohoku University

    2018年 ~ 2020年

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    暑熱環境下ではニワトリの増体量は著しく低下するが、そのメカニズムは未だ不明である。本課題では、ニワトリの暑熱時の不利益反応および暑熱への適応応答を明確化することを目的とした。夏季および秋季に採取した種卵より得たニワトリを同一条件で暑熱感作した結果、秋卵ニワトリでは体温、血漿中コルチコステロン濃度、尿酸値、インターロイキン6濃度が上昇したが、夏卵ニワトリではこれらの上昇が低く抑えられることが示された。さらに、メタボロミクス解析の結果、抗炎症物質である3-ヒドロキシ酪酸量が夏卵ニワトリで高く維持されていることが示された。以上より、ニワトリの暑熱耐性には抗炎症力の大小が関与することが示唆された。

  6. 動物の暑熱ストレス耐性向上のための包括的研究:生体と腸内細菌叢の統合制御

    豊水 正昭, 大塚 彰, 陶山 佳久, 喜久里 基

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:Tohoku University

    2015年4月 ~ 2018年3月

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    本研究では、暑熱下の肉用鶏の生産性低下の機序を明らかにし、生産性改善のための飼料資材を提案する。暑熱感作した鶏では、骨格筋で発生する活性酸素により骨格筋タンパク質分解は亢進し、脾臓ではT細胞数の低下が観察され、代謝と免疫システムの恒常性のためエネルギー要求が高まった。なお、暑熱感作後期で回腸ではStreptococcus属が減少、Natronincola属が増加傾向を示した。カシューナッツ殻油CNSL給与による生産性改善を検討したところ、暑熱CNSL給与で改善傾向にあった。暑熱感作による腸管の免疫関連遺伝子発現の上昇がCNSL給与で緩和されており、CNSLの生産性改善効果の一部が説明された。

  7. 新規未利用飼料資材によるストレス環境下における安定的肉用鶏生産技術の開発 競争的資金

    制度名:Contract Research

    2013年4月 ~ 2017年3月

  8. 飼料用米の給与による畜産物の差別化技術及び家畜の健全性向上技術の開発 競争的資金

    制度名:The Other Research Programs

    2015年4月 ~ 2016年3月

  9. 動物のストレス耐性向上のための包括的研究:酸化ストレス・代謝・免疫系の統合的制御 競争的資金

    豊水 正昭, 高橋 和昭, 大津 晴彦, 喜久里 基

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:Tohoku University

    2012年4月1日 ~ 2015年3月31日

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    本研究は暑熱・内毒素ストレスが肉用鶏の生体におよぼす影響を調べた。慢性的な暑熱環境下では、骨格筋・肝臓ともに酸化ストレスが亢進したが、前者では抗酸化システムが活性化していたのに対し、後者ではこれが認められなかった。LPS投与時では、両組織において酸化ストレスは亢進しなかったものの、炎症性サイトカインの遺伝子発現量が増加した。抗酸化力に優れる電解還元水および有機セレン給与により、暑熱時の酸化ストレスは抑制されたものの増体量は回復しなかった。以上の結果より、暑熱ストレス時の増体量の回復は酸化ストレス制御のみでは十分ではなく、他の要因例えば腸内細菌叢の最適化なども重要であることが示された。

  10. 暑熱負荷時の鶏の代謝時系列変化およびその連動性の解明 競争的資金

    喜久里 基

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    研究種目:Grant-in-Aid for Young Scientists (B)

    研究機関:Tohoku University

    2013年4月 ~ 2015年3月

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    本研究では暑熱環境下におけるニワトリのミトコンドリア活性酸素(mtROS)産生および体タンパク質分解システムの関連性を調べた。暑熱感作後、早期段階においてmtROS産生およびユビキチン転移酵素(E3)のmRNA発現量が増加し、その後、血中3メチルヒスチジン含量が増加した。続いて、ニワトリ筋細胞を高温感作した結果、mtROS産生量・E3 mRNA発現量の同時上昇が認められた。高温感作時のmtROS産生をTempolで抑制した結果、E3のmRNA発現量は低下し、細胞タンパク質量も通常状態まで回復した。以上より、暑熱環境下ではmtROS産生がタンパク質分解亢進の引き金になっていることが示された。

  11. 鶏の暑熱適応における脱共役タンパク質の生理的役割および発現制御メカニズムの解明

    喜久里 基

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up

    研究種目:Grant-in-Aid for Research Activity Start-up

    研究機関:Tohoku University

    2011年 ~ 2012年

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    これまでに研究代表者らは、急性暑熱感作にともなう肉用鶏の骨格筋ミトコンドリアにおける活性酸素種(reactive oxygen species、ROS)の過剰産生は、膜電位上昇によってもたらされること、さらにこの上昇には鶏特異的脱共役タンパク質(avian uncoupling protein、avUCP)の発現低下にともなうプロトンリーク反応の低下が関与していることを明らかにしている。本研究では、先天的にavUCP発現が高い産卵鶏ならびにオリーブオイル+オレウロペイン給与によってavUCP発現を上昇させた肉用鶏を急性暑熱感作しても、これら鶏の骨格筋ではミトコンドリアROS過剰産生が抑制されることを実証し、avUCPが暑熱時の酸化ストレス発症抑制に重要な役割を果たすことを明らかにした。

  12. 暑熱ストレスが引き起こすROS過剰産生機構の解明とその制御法の開発

    喜久里 基

    2008年 ~ 2010年

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    夏季などの暑熱環境下において、鶏は「酸化ストレス」状態に陥る。これにより、生産性が著しく低下すると考えられるが、酸化ストレスの発現機序は未だ明らかでなく、また、有効的な飼養技術も確立されていない。本研究では、急性暑熱時の酸化ストレス誘導因子であるミトコンドリア活性酸素種(mitROS)の過剰産生機構を明らかにするとともに、酸化ストレスを低減する機能性栄養資材を探索することを目的とする。本年度は、後者すなわち機能性資材コエンザイムQ_<10>(CoQ_<10>)を給与し、暑熱時の酸化ストレスに対する緩和効果を調べた。 CoQ_<10>は、ROSを捕捉する抗酸化物質であり、ミトコンドリア電子伝達鎖の構成因子でもある。このため、CoQ_<10>給与により、抗酸化のみならずmitROS過剰産生抑制の面からも酸化ストレス低減効果が期待できる。実験では、CoQ_<10>添加飼料(40mg/kg飼料)を10日齢より11日間、雄肉用鶏に自由摂取させた後、25℃を対照区として34℃、12時間急性暑熱曝露した(暑熱区)。酸化ストレスマーカーであるマロンジアルデヒド(MDA)およびカルボニル化タンパク質含量を調べた結果、暑熱曝露によって、血漿MDA含量ならびに浅胸筋カルボニル化タンパク質含量は、通常飼料区では有意に増加したが、CoQ_<10>飼料区ではほとんど変化しなかった。血漿中の総抗酸化能は、暑熱感作およびCoQ_<10>給与のいずれによってもほとんど変化しなかった。これに対し、浅胸筋のmitROS産生量は、FADH_2供給時において暑熱によって著しく増加したが、CoQ_<10>給与によりこの増加は抑えられた。以上の結果より、急性暑熱時において、CoQ_<10>は鶏骨格筋mitROS過剰産生を抑制し、酸化ストレスを低減することが実証された。したがって、CoQ_<10>は暑熱時の酸化ストレス緩和において有効な栄養資材になり得る可能性が示された。

︎全件表示 ︎最初の5件までを表示

社会貢献活動 1

  1. 出張講義

    2016年5月13日 ~

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    仙台市泉高校において、畜産学の出張講義を行った。

その他 5

  1. レシチンが肉用鶏の飼養成績におよぼす影響

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    飼料に脂質レチシンを加えた際における肉用鶏の飼養成績および代謝試験成績(脂肪消化率、粗タンパク質消化率)におよぼす影響を調べた。

  2. 植物抽出物が暑熱ニワトリの飼養成績におよぼす影響

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    植物抽出物が暑熱ニワトリの飼養成績や酸化ストレスにおよぼす影響を調べた。

  3. 枯草菌給与が鶏肉品質におよぼす影響

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    枯草菌(カルスポリン)給与が鶏肉品質(酸化損傷度、ビタミンE含量、水分保持力)におよぼす影響を調べた。

  4. 飼料用米の給与による畜産物の差別化技術及び家畜の健全性向上技術の開発

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    国内自給率を目的とし、飼料用米の給与による畜産物の差別化技術及び家畜の健全性向上技術の開発を行う。

  5. 温暖化の進行に適応する畜産の生産安定技術の開発 肉用鶏での栄養管理に基づく安定生産のための暑熱対策技術の研究開発

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    暑熱環境下における家畜の生体応答を把握し、現場レベルでの生産性改善方法を模索する。