顔写真

タケウチ ヒデアキ
竹内 秀明
Hideaki Takeuchi
所属
大学院生命科学研究科 脳生命統御科学専攻 神経ネットワーク講座(分子行動分野)
職名
教授
学位
  • 博士(薬学)(東京大学)

  • 修士(薬学)(東京大学)

e-Rad 研究者番号
00376534
プロフィール
To clarify both molecular/neural basis and brain information processing underlying social interactions in vertebrates, we have focused on Medaka fish and established novel behavior systems to assess social interactions (schooling behavior, individual recognition, group-escape and group-learning). Using these systems we are planning to identify internal factors (genes, neural networks, and brain regions) essential for vertebrate social interactions using transgenic medaka systems. On the other hand, to estimate behavior rules underlying social interactions, we developed a hypothesis-independent data mining, which could explain actual fish movement. Our eventual purpose is to how the internal factors (genes, neural networks, and brain regions) influences animal behaviors which can serve as the basis for the emergence of sociality.
Furthermore I have investigated the molecular/neural basis underlying invertebrate social behavior (honeybee) and compare neural/ social mechanisms between the vertebrate and invertebrate.

経歴 19

  • 2022年4月 ~ 継続中
    宇都宮大学 オプティクス教育研究センター 客員教授

  • 2019年11月 ~ 継続中
    東北大学 大学院生命科学研究科 教授

  • 2019年11月 ~ 2021年3月
    岡山大学 大学院自然科学研究科 特任教授

  • 2015年4月 ~ 2019年10月
    岡山大学 大学院自然科学研究科 准教授

  • 2006年4月 ~ 2015年3月
    東京大学 大学院理学系研究科 生物科学専攻 助教

  • 2004年4月 ~ 2015年3月
    東京大学 大学院理学系研究科 生物科学専攻

  • 2004年4月 ~ 2006年3月
    東京大学大学院理学系研究科生物科学専攻 助手

  • 2003年4月 ~ 2004年3月
    東京大学大学院理学系研究科 産学官連携研究員

  • 1999年4月 ~ 2004年3月
    Postdoctral fellow (Supervisor: Prof. Takeo Kubo)

  • 2000年5月 ~ 2003年3月
    生物系特定産業技術研究推進機構 派遣研究員

  • 2000年4月 ~ 2000年5月
    日本学術振興会未来開拓事業 リサーチアソシエイト

  • 1999年4月 ~ 2000年3月
    日本学術振興会平成10年度採用特別研究員 PD

  • 1998年4月 ~ 1999年3月
    日本学術振興会平成10年度採用特別研究員 DC2

  • 1996年4月 ~ 1999年3月
    東京大学 大学院薬学系研究科

  • 1996年4月 ~ 1999年3月
    東京大学大学院薬学系研究科博士課程機能薬学専攻修了 博士(薬学)

  • 1994年4月 ~ 1996年3月
    東京大学 大学院薬学系研究科

  • 1994年4月 ~ 1996年3月
    東京大学大学院薬学系研究科修士課程機能薬学専攻修了

  • 1992年4月 ~ 1994年3月
    東京大学 薬学部

  • 1992年4月 ~ 1994年3月
    東京大学薬学部卒業

︎全件表示 ︎最初の5件までを表示

所属学協会 3

  • 日本行動神経内分泌研究会

  • 日本神経科学学会

  • 日本動物学会

研究キーワード 6

  • 包括脳ネットワーク

  • 神経

  • 行動生物学

  • 分子生物学

  • 社会性行動

研究分野 1

  • ライフサイエンス / 神経科学一般 / 行動生物学、分子生物学

論文 83

  1. Rapid body colouration change inOryzias celebensisas a social signal for intraspecific competition

    Ryutaro Ueda, Satoshi Ansai, Hideaki Takeuchi

    2023年12月24日

    DOI: 10.1101/2023.12.23.573172  

  2. Epigenetically distinct synaptic architecture in clonal compartments in the teleostean dorsal pallium. 国際誌

    Yasuko Isoe, Ryohei Nakamura, Shigenori Nonaka, Yasuhiro Kamei, Teruhiro Okuyama, Naoyuki Yamamoto, Hideaki Takeuchi#, Hiroyuki Takeda#, #These authors have equally supervised the work

    eLife 12 2023年7月25日

    DOI: 10.7554/eLife.85093  

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    The dorsal telencephalon (i.e. the pallium) exhibits high anatomical diversity across vertebrate classes. The non-mammalian dorsal pallium accommodates various compartmentalized structures among species. The developmental, functional, and evolutional diversity of the dorsal pallium remain unillustrated. Here, we analyzed the structure and epigenetic landscapes of cell lineages in the telencephalon of medaka fish (Oryzias latipes) that possesses a clearly delineated dorsal pallium (Dd2). We found that pallial anatomical regions, including Dd2, are formed by mutually exclusive clonal units, and that each pallium compartment exhibits a distinct epigenetic landscape. In particular, Dd2 possesses a unique open chromatin pattern that preferentially targets synaptic genes. Indeed, Dd2 shows a high density of synapses. Finally, we identified several transcription factors as candidate regulators. Taken together, we suggest that cell lineages are the basic components for the functional regionalization in the pallial anatomical compartments and that their changes have been the driving force for evolutionary diversity.

  3. Optogenetic control of medaka behavior with channelrhodopsin.

    Takahide Seki, Hideaki Takeuchi, Satoshi Ansai

    Development, growth & differentiation 2023年6月24日

    DOI: 10.1111/dgd.12872  

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    Optogenetics enables the manipulation of neural activity with high spatiotemporal resolution in genetically defined neurons. The method is widely used in various model animals in the neuroscience and physiology fields. Channelrhodopsins are robust tools for optogenetic manipulation, but they have not yet been used for studies in medaka. In the present study, we used the CRISPR/Cas9-mediated knock-in approach to establish a transgenic medaka strain expressing the Chloromonas oogama channelrhodopsin (CoChR) in the ISL LIM homeobox 1 (isl1) locus. We demonstrated that light stimuli elicited specific behavioral responses such as bending or turning locomotion in the embryos and pectoral fin movements in the larvae and adults. The response probabilities and intensities of these movements could be controlled by adjusting the intensity, duration, or wavelength of each light stimulus. Furthermore, we demonstrated that the pectoral fin movements in the adult stage could be elicited using a laser pointer to irradiate region including the caudal hind brain and the rostral spinal cord. Our results indicate that CoChR allows for manipulation of medaka behaviors by activating targeted neurons, which will further our understanding of the detailed neural mechanisms of motor control or social behaviors in medaka. This article is protected by copyright. All rights reserved.

  4. Spatio-temporal control of targeted gene expression in combination with CRISPR/Cas and Tet-On systems in Medaka. 国際誌

    Daichi Kayo, Sayaka Kimura, Touko Yamazaki, Kiyoshi Naruse, Hideaki Takeuchi, Satoshi Ansai

    Genesis (New York, N.Y. : 2000) e23519 2023年5月25日

    DOI: 10.1002/dvg.23519  

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    Spatial and temporal control of transgene expression is a powerful approach to understand gene functions in specific cells and tissues. The Tet-On system is a robust tool for controlling transgene expression spatially and temporally; however, few studies have examined whether this system can be applied to postembryonic stages of Medaka (Oryzias latipes) or other fishes. Here, we first improved a basal promoter sequence on the donor vector for a nonhomologous end joining (NHEJ)-based knock-in (KI) system. Next, using transgenic Medaka for establishing the Tet-On system by KI, we demonstrated that doxycycline administration for four or more days by feeding can be a stable and efficient method to achieve expression of the transduced reporter gene in adult fish. From these analyses, we propose an optimized approach for a spatio-temporal gene-expression system in the adult stage of Medaka and other small fishes.

  5. Mating experiences with the same partner enhanced mating activities of naïve male medaka fish. 国際誌

    Masahiro Daimon, Takafumi Katsumura, Hirotaka Sakamoto, Satoshi Ansai, Hideaki Takeuchi

    Scientific reports 12 (1) 19665-19665 2022年11月16日

    DOI: 10.1038/s41598-022-23871-w  

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    Mating experience shapes male mating behavior across species, from insects, fish, and birds, to rodents. Here, we investigated the effect of multiple mating experiences on male mating behavior in "naïve" (defined as sexually inexperienced) male medaka fish. The latency to mate with the same female partner significantly decreased after the second encounter, whereas when the partner was changed, the latency to mate was not decreased. These findings suggest that mating experiences enhanced the mating activity of naïve males for the familiar female, but not for an unfamiliar female. In contrast, the mating experiences of "experienced" (defined as those having mated > 7 times) males with the same partner did not influence their latency to mate. Furthermore, we identified 10 highly and differentially expressed genes in the brains of the naïve males after the mating experience and revealed 3 genes that are required for a functional cascade of the thyroid hormone system. Together, these findings suggest that the mating experience of naïve male medaka fish influences their mating behaviors, with neural changes triggered by thyroid hormone activation in the brain.

  6. Collective Decision-making in Response to Visual Looming Stimuli in Medaka Fish

    Ryohei Nakahata, Hideaki Takeuchi

    2022年6月16日

    出版者・発行元:Cold Spring Harbor Laboratory

    DOI: 10.1101/2022.06.14.494464  

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    Various social animals synchronously make behavioral choices among alternatives (i.e., collective decision-making) to efficiently migrate, forage, or escape from predators. Here, we demonstrated that medaka fish, Oryzias latipes, make collective decisions in response to visual looming stimuli (LS), a simulated predator attack. The group-level response to the LS could be classified into 2 patterns: freezing synchronously or not freezing, indicating that medaka selected their response to the LS from 2 alternative choices. Furthermore, the behavioral response to the LS persisted over multiple behavioral trials for 3 days, indicating that each group made a consistent decision to freeze or not to freeze. Finally, we showed that the group response could be predicted by the maximum swimming speed among individuals during the LS presentation. If a group included at least 1 individual that responded to the LS with high-speed movement (>7.4 cm/s), the group tended to freeze synchronously in response to the LS. If no member of the group was moving at high-speed during the LS presentation, the group tended to continue moving after the LS presentation. Our findings suggest that the presence of 1 or more individual medaka with a strong fear response in a group determines the group-level decision of how to respond to a threatening stimulus.

  7. Quantifying social Interactions in medaka fish

    Yokoi S, Isoe Y, Wang MY, Daimon M, Okuyama T, Takeuchi H

    Behavioral Neurogenetics in press 93-105 2022年

    DOI: 10.1007/978-1-0716-2321-3_7  

    ISSN:0893-2336

    eISSN:1940-6045

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    Zebrafish (Danio rerio) and medaka fish (Oryzias latipes) are popular vertebrate models for various biology fields. Medaka fish have recently attracted attention in the field of social neuroscience due to some of their unique advantages. First, medaka fish females have an extremely short ovarian cycle (24 h), which allows one to obtain a large number of female individuals of a similar physiological status according to their synchronized reproductive rhythms, facilitating quantitative analysis of female behaviors that are strongly influenced by the ovarian cycle; other experimental models such as mice and zebrafish have a longer ovarian cycle and/or unpredictable ovarian maturation. Second, medaka fish possess the visual ability to recognize conspecifics and make appropriate behavioral choices based on their social relationship. Third, the medaka fish are an established model in the field of molecular genetics (as are zebrafish) and state-of-the-art molecular-genetic methods are available. Here we describe several experimental procedures for behavioral paradigms to quantify medaka social behaviors, which are primarily mediated by visual cues.

  8. A modified Tet-ON system minimizing leaky expression for cell-type specific gene induction in medaka fish.

    Osamu Hosoya, Myung Chung, Satoshi Ansai, Hideaki Takeuchi, Mary Miyaji

    Development, growth & differentiation 2021年8月10日

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    The Tet-ON system is an important molecular tool for temporally and spatially-controlled inducible gene expression. Here, we developed a Tet-ON system to induce transgene expression specifically in the rod photoreceptors of medaka fish. Our modified reverse tetracycline-controlled transcriptional transactivator (rtTAm) with 5 amino acid substitutions dramatically improved the leakiness of the transgene in medaka fish. We generated a transgenic line carrying a self-reporting vector with the rtTAm gene driven by the Xenopus rhodopsin promoter and a tetracycline response element (TRE) followed by the green fluorescent protein (GFP) gene. We demonstrated that GFP fluorescence was restricted to the rod photoreceptors in the presence of doxycycline in larval fish (9 days post-fertilization). The GFP fluorescence intensity was enhanced with longer durations of doxycycline treatment up to 72 h and in a dose-dependent manner (5-45 μg/ml). These findings demonstrate that the Tet-ON system using rtTAm allows for spatiotemporal control of transgene expression, at least in the rod photoreceptors, in medaka fish.

  9. Adrenomedullin 2 and 5 activate the calcitonin receptor-like receptor (clr) - Receptor activity-modifying protein 3 (ramp3) receptor complex in Xenopus tropicalis. 国際誌

    Maho Ogoshi, Mikoto Takahashi, Kota Aoyagi, Kazuyoshi Ukena, Sayaka Aizawa, Hideaki Takeuchi, Sumio Takahashi, Sakae Takeuchi

    General and comparative endocrinology 306 113752-113752 2021年5月15日

    DOI: 10.1016/j.ygcen.2021.113752  

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    The adrenomedullin (AM) family is involved in diverse biological functions, including cardiovascular regulation and body fluid homeostasis, in multiple vertebrate lineages. The AM family consists of AM1, AM2, and AM5 in tetrapods, and the receptor for mammalian AMs has been identified as the complex of calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 2 (RAMP2) or RAMP3. However, the receptors for AM in amphibians have not been identified. In this study, we identified the cDNAs encoding calcrl (clr), ramp2, and ramp3 receptor components from the western clawed frog (Xenopus tropicalis). Messenger RNAs of amphibian clr and ramp2 were highly expressed in the heart, whereas that of ramp3 was highly expressed in the whole blood. In HEK293T cells expressing clr-ramp2, cAMP response element luciferase (CRE-Luc) reporter activity was activated by am1. In HEK293T cells expressing clr-ramp3, CRE-Luc reporter activity was increased by the treatment with am2 at the lowest dose, but with am5 and am1 at higher dose. Our results provided new insights into the roles of AM family peptides through CLR-RAMP receptor complexes in the tetrapods.

  10. Sexually dimorphic role of oxytocin in medaka mate choice. 国際誌 査読有り

    Saori Yokoi, Kiyoshi Naruse, Yasuhiro Kamei, Satoshi Ansai, Masato Kinoshita, Mari Mito, Shintaro Iwasaki, Shuntaro Inoue, Teruhiro Okuyama, Shinichi Nakagawa, Larry J Young, Hideaki Takeuchi

    Proceedings of the National Academy of Sciences of the United States of America 117 (9) 4802-4808 2020年3月3日

    DOI: 10.1073/pnas.1921446117  

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    Oxytocin is a central neuromodulator required for facilitating mate preferences for familiar individuals in a monogamous rodent (prairie vole), irrespective of sex. While the role of oxytocin in mate choice is only understood in a few monogamous species, its function in nonmonogamous species, comprising the vast majority of vertebrate species, remains unclear. To address this issue, we evaluated the involvement of an oxytocin homolog (isotocin, referred herein as oxt) in mate choice in medaka fish (Oryzias latipes). Female medaka prefer to choose familiar mates, whereas male medaka court indiscriminately, irrespective of familiarity. We generated mutants of the oxt ligand (oxt) and receptor genes (oxtr1 and oxtr2) and revealed that the oxt-oxtr1 signaling pathway was essential for eliciting female mate preference for familiar males. This pathway was also required for unrestricted and indiscriminate mating strategy in males. That is, either oxt or oxtr1 mutation in males decreased the number of courtship displays toward novel females, but not toward familiar females. Further, males with these mutations exhibited enhanced mate-guarding behaviors toward familiar females, but not toward novel females. In addition, RNA-sequencing (seq) analysis revealed that the transcription of genes involved in gamma-amino butyric acid metabolism as well as those encoding ion-transport ATPase are up-regulated in both oxt and oxtr1 mutants only in female medaka, potentially explaining the sex difference of the mutant phenotype. Our findings provide genetic evidence that oxt-oxtr1 signaling plays a role in the mate choice for familiar individuals in a sex-specific manner in medaka fish.

  11. Development of omnidirectional aerial display with aerial imaging by retro-reflection (AIRR) for behavioral biology experiments 査読有り

    Optical Review 26 (1) 221-229 2019年2月

    出版者・発行元:None

    DOI: 10.1007/s10043-019-00502-w  

    ISSN:1340-6000

    eISSN:1349-9432

  12. Clonal analysis of construction mechanism of the adult telencephalon mediated by post-hatch neurogenesiss in medaka fish 査読有り

    Yasuko Isoe, Ryohei Nakamura, Yasuhiro Kamei, Shigenori Nonaka, Teruhiro Okuyama, Atsushi Shimizu, Takeo Kubo, Hiroyuki Takeda, Hideaki Takeuchi

    Mechanisms of Development 145 S118-S119 2017年7月

    出版者・発行元:Elsevier {BV}

    DOI: 10.1016/j.mod.2017.04.320  

    ISSN:0925-4773

    eISSN:1872-6356

  13. Individual recognition and the 'face inversion effect' in medaka fish (Oryzias latipes) 査読有り

    Mu-Yun Wang, Hideaki Takeuchi

    ELIFE 6 2017年7月

    出版者・発行元:ELIFE SCIENCES PUBLICATIONS LTD

    DOI: 10.7554/eLife.24728  

    ISSN:2050-084X

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    Individual recognition (IR) is essential for maintaining various social interactions in a group, and face recognition is one of the most specialised cognitive abilities in IR. We used both a mating preference system and an electric shock conditioning experiment to test IR ability in medaka, and found that signals near the face are important. Medaka required more time to discriminate vertically inverted faces, but not horizontally shifted faces or inverted non-face objects. The ability may be comparable to the classic 'face inversion effect' in humans and some other mammals. Extra patterns added to the face also did not influence the IR. These findings suggest the possibility that the process of face recognition may differ from that used for other objects. The complex form of recognition may promote specific processing adaptations, although the mechanisms and neurological bases might differ in mammals and medaka. The ability to recognise other individuals is important for shaping animal societies.

  14. Molecular basis of social competence in medaka fish. 査読有り

    Teruhiro Okuyama, Saori Yokoi, Hideaki Takeuchi

    Development, growth & differentiation 59 (4) 211-218 2017年5月

    DOI: 10.1111/dgd.12359  

    ISSN:0012-1592

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    Oryzias latipes (Medaka) is an established vertebrate model for studying developmental genetics, genomics, and evolutionary biology. The physiology, embryology, and genetics of this species have been extensively investigated for centuries. Medaka fish recently attracted attention in the field of social neuroscience. This review introduces recent advances in medaka behavioral studies, focusing on female mating preferences and male mate-guarding behaviors. The medaka female has the ability to discriminate male individuals and prefers to mate with socially familiar males (female mating preference). In triadic relationships (two males and one female), the dominant male remains closer to the female and repels the other male (mate-guarding). Interestingly, mate-guarding blocks female social familiarization of the rival male, which can increase the mating success of the dominant male. Importantly, behavioral analyses using a series of medaka mutants revealed critical roles of neuropeptide neuromodulatory systems in regulating their social behaviors. The extra-hypothalamic gonadotropin-releasing hormone system has a central role in activating female mating preference. The arginine-vasotocin system is required for the emergence of mate-guarding behavior.

  15. メダカにおいてバソトシン系は配偶者防衛行動を制御する(Vasotocin system regulates mate-guarding behavior in medaka fish)

    Yokoi Saori, Okuyama Teruhiro, Ansai Satoshi, Kamei Yasuhiro, Taniguchi Yoshihito, Kinoshita Masato, Young Larry J., Takemori Nobuaki, Kubo Takeo, Naruse Kiyoshi, Takeuchi Hideaki

    比較生理生化学 33 (4) 149-149 2016年12月

    出版者・発行元:日本比較生理生化学会

    ISSN:0916-3786

    eISSN:1881-9346

  16. Production of Knockout Mutants by CRISPR/Cas9 in the European Honeybee, Apis mellifera L. 査読有り

    Hiroki Kohno, Shota Suenami, Hideaki Takeuchi, Tetsuhiko Sasaki, Takeo Kubo

    ZOOLOGICAL SCIENCE 33 (5) 505-512 2016年10月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    DOI: 10.2108/zs160043  

    ISSN:0289-0003

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    The European honeybee (Apis mellifera L.) is used as a model organism in studies of the molecular and neural mechanisms underlying social behaviors and/or advanced brain functions. The entire honeybee genome has been sequenced, which has further advanced molecular biologic studies of the honeybee. Functions of genes of interest, however, remain largely to be elucidated in the honeybee due to the lack of effective reverse genetic methods. Moreover, genetically modified honeybees must be maintained under restricted laboratory conditions due to legal restrictions, further complicating the application of reverse genetics to this species. Here we applied CRISPR/Cas9 to the honeybee to develop an effective reverse genetic method. We targeted major royal jelly protein 1 (mrjp1) for genome editing, because this gene is predominantly expressed in adult workers and its mutation is not expected to affect normal development. By injecting sgRNA and Cas9 mRNA into 57 fertilized embryos collected within 3 h after oviposition, we successfully created six queens, one of which produced genome-edited male offspring. Of the 161 males produced, genotyping demonstrated that the genome was edited in 20 males. All of the processes necessary for producing these genome-edited queens and males were performed in the laboratory. Therefore, we developed essential techniques to create knockout honeybees by CRISPR/Cas9. Our findings also suggested that mrjp1 is dispensable for normal male development, at least till the pupal stage. This new technology could pave the way for future functional analyses of candidate genes involved in honeybee social behaviors.

  17. Ontogeny and Sexual Differences in Swimming Proximity to Conspecifics in Response to Visual Cues in Medaka Fish 査読有り

    Yasuko Isoe, Yumi Konagaya, Saori Yokoi, Takeo Kubo, Hideaki Takeuchi

    ZOOLOGICAL SCIENCE 33 (3) 246-254 2016年6月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    DOI: 10.2108/zs150213  

    ISSN:0289-0003

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    Adult medaka fish (Oryzias latipes) exhibit complex social behaviors that depend mainly on visual cues from conspecifics. The ontogeny of visually-mediated social behaviors from larval/juvenile to adult medaka fish, however, is unknown. In the present study, we established a simple behavioral paradigm to evaluate the swimming proximity to conspecifics based on visual cues in an inter-individual interaction of two medaka fish throughout life. When two fish were placed separately in a cylindrical tank with a concentric transparent wall, the two fish maintained close proximity to each other. A normal fish inside the tank maintained proximity to an optic nerve-cut fish outside of the tank, while the converse was not true. This behavioral paradigm enabled us to quantify visually-induced motivation of a single fish inside the tank. The proximity was detected from larval/juvenile to adult fish. Larval fish, however, maintained close proximity not only to conspecifics, but also to heterospecifics. As the growth stage increased, the degree of proximity to heterospecifics decreased, suggesting that shoaling preferences toward conspecifics and/or visual ability to recognize conspecifics is refined and established according to the growth stage. Furthermore, the proximity of adult female fish was affected by their reproductive status and social familiarity. Only before spawning, adult females maintained closer proximity to familiar males rather than to unfamiliar males, suggesting that proximity was affected by familiarity in a female-specific manner. This simple behavioral paradigm will contribute to our understanding of the neural basis of the development of visually-mediated social behavior using medaka fish.

  18. Analysis of the Differentiation of Kenyon Cell Subtypes Using Three Mushroom Body-Preferential Genes during Metamorphosis in the Honeybee (Apis mellifera L.) 査読有り

    Shota Suenami, Rajib Kumar Paul, Hideaki Takeuchi, Genta Okude, Tomoko Fujiyuki, Kenichi Shirai, Takeo Kubo

    PLOS ONE 11 (6) e0157841 2016年6月

    出版者・発行元:PUBLIC LIBRARY SCIENCE

    DOI: 10.1371/journal.pone.0157841  

    ISSN:1932-6203

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    The adult honeybee (Apis mellifera L.) mushroom bodies (MBs, a higher center in the insect brain) comprise four subtypes of intrinsic neurons: the class-I large-, middle-, and small-type Kenyon cells (lKCs, mKCs, and sKCs, respectively), and class-II KCs. Analysis of the differentiation of KC subtypes during metamorphosis is important for the better understanding of the roles of KC subtypes related to the honeybee behaviors. In the present study, aiming at identifying marker genes for KC subtypes, we used a cDNA microarray to comprehensively search for genes expressed in an MB-preferential manner in the honeybee brain. Among the 18 genes identified, we further analyzed three genes whose expression was enriched in the MBs: phospholipase C epsilon (PLCe), synaptotagmin 14 (Syt14), and discs large homolog 5 (dlg5). Quantitative reverse transcription-polymerase chain reaction analysis revealed that expression of PLCe, Syt14, and dlg5 was more enriched in the MBs than in the other brain regions by approximately 31-, 6.8-, and 5.6-fold, respectively. In situ hybridization revealed that expression of both Syt14 and dlg5 was enriched in the lKCs but not in the mKCs and sKCs, whereas expression of PLCe was similar in all KC subtypes (the entire MBs) in the honeybee brain, suggesting that Syt14 and dlg5, and PLCe are available as marker genes for the lKCs, and all KC subtypes, respectively. In situ hybridization revealed that expression of PLCe is already detectable in the class-II KCs at the larval fifth instar feeding stage, indicating that PLCe expression is a characteristic common to the larval and adult MBs. In contrast, expression of both Syt14 and dlg5 became detectable at the day three pupa, indicating that Syt14 and dlg5 expressions are characteristic to the late pupal and adult MBs and the lKC specific molecular characteristics are established during the late pupal stages.

  19. Mate-guarding behavior enhances male reproductive success via familiarization with mating partners in medaka fish. 国際誌 査読有り

    Saori Yokoi, Satoshi Ansai, Masato Kinoshita, Kiyoshi Naruse, Yasuhiro Kamei, Larry J Young, Teruhiro Okuyama, Hideaki Takeuchi

    Frontiers in zoology 13 21-21 2016年

    DOI: 10.1186/s12983-016-0152-2  

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    BACKGROUND: Male-male competition and female mating preference are major mechanisms of sexual selection, which influences individual fitness. How male-male competition affects female preference, however, remains poorly understood. Under laboratory conditions, medaka (Oryzias latipes) males compete to position themselves between a rival male and the female (mate-guarding) in triadic relationships (male, male, and female). In addition, females prefer to mate with visually familiar males. In the present study, to examine whether mate-guarding affects female preference via visual familiarization, we established a novel behavioral test to simultaneously quantify visual familiarization of focal males with females and mate-guarding against rival males. In addition, we investigated the effect of familiarization on male reproductive success in triadic relationships. RESULTS: Three fish (female, male, male) were placed separately in a transparent three-chamber tank, which allowed the male in the center (near male) to maintain closer proximity to the female than the other male (far male). Placement of the wild-type male in the center blocked visual familiarization of the far male by the female via mate-guarding. In contrast, placement of an arginine-vasotocin receptor mutant male, which exhibits mate-guarding deficits, in the center, allowing for maintaining close proximity to the female, did not block familiarization of the far male by the female. We also demonstrated that the reproductive success of males was significantly decreased by depriving females visual familiarization with the males. CONCLUSIONS: Our findings indicated that, at least in triadic relationships, dominance in mate-guarding, not simply close proximity, allows males to gain familiarity with the female over their rivals, which may enhance female preference for the dominant male. These findings focusing on the triadic relationships of medaka may contribute to our understanding of the adaptive significance of persistent mate-guarding, as well as female preference for familiar mates.

  20. [Exploring evolutionary roots of "social brain": Central role of terminal nerve GnRH3 neurons in social decision making]. 査読有り

    Teruhiro Okuyama, Hideaki Takeuchi

    Seikagaku. The Journal of Japanese Biochemical Society 87 (5) 605-8 2015年10月

    ISSN:0037-1017

  21. Changes in the Gene Expression Profiles of the Hypopharyngeal Gland of Worker Honeybees in Association with Worker Behavior and Hormonal Factors 査読有り

    Takayuki Ueno, Hideaki Takeuchi, Kiyoshi Kawasaki, Takeo Kubo

    PLOS ONE 10 (6) e0130206 2015年6月

    出版者・発行元:PUBLIC LIBRARY SCIENCE

    DOI: 10.1371/journal.pone.0130206  

    ISSN:1932-6203

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    The hypopharyngeal glands (HPGs) of worker honeybees undergo physiological changes along with the age-dependent role change from nursing to foraging: nurse bee HPGs secrete mainly major royal jelly proteins, whereas forager HPGs secrete mainly a-glucosidase III, which converts the sucrose in the nectar into glucose and fructose. We previously identified two other genes, Apis mellifera buffy(Ambuffy) and Apis mellifera matrix metalloproteinase 1(AmMMP1), with enriched expression in nurse bee and forager HPGs, respectively. In the present study, to clarify the molecular mechanisms that coordinate HPG physiology with worker behavior, we first analyzed whether Ambuffy, AmMMP1, mrjp2(a gene encoding one of major royal jelly protein isoforms), and Hbg3 (a gene encoding a-glucosidase III) expression, is associated with worker behavior in 'single-cohort colonies' where workers of almost the same age perform different tasks. Expression of these genes correlated with the worker's role, while controlling for age, indicating their regulation associated with the worker's behavior. Associated gene expression suggested the possible involvement of some hormonal factors in its regulation. We therefore examined the relationship between ecdysone- and juvenile hormone (JH)-signaling, and the expression profiles of these 'indicator' genes (nurse bee HPG-selective genes: mrjp2 and Ambuffy, and forager HPG-selective genes: Hbg3and AmMMP1). Expression of both ecdysone-regulated genes (ecdysone receptor, mushroom body large type Kenyon cell specific protein-1, and E74) and JH-regulated genes (Methoprene tolerant and Kruppel homolog 1) was higher in the forager HPGs than in the nurse bee HPGs, suggesting the possible roles of ecdysone-and JH-regulated genes in worker HPGs. Furthermore, 20-hydroxyecdysone-treatment repressed both nurse bee-and forager-selective gene expression, whereas methoprene-treatment enhanced the expression of forager-selective genes and repressed nurse bee-selective genes in the HPGs. Our findings suggest that both ecdysone-and JH-signaling cooperatively regulate the physiological state of the HPGs in association with the worker's behavior.

  22. An Essential Role of the Arginine Vasotocin System in Mate-Guarding Behaviors in Triadic Relationships of Medaka Fish (Oryzias latipes) 査読有り

    Saori Yokoi, Teruhiro Okuyama, Yasuhiro Kamei, Kiyoshi Naruse, Yoshihito Taniguchi, Satoshi Ansai, Masato Kinoshita, Larry J. Young, Nobuaki Takemori, Takeo Kubo, Hideaki Takeuchi

    PLOS GENETICS 11 (2) 2015年2月

    出版者・発行元:PUBLIC LIBRARY SCIENCE

    DOI: 10.1371/journal.pgen.1005009  

    ISSN:1553-7404

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    To increase individual male fitness, males of various species remain near a (potential) mating partner and repel their rivals (mate-guarding). Mate-guarding is assumed to be mediated by two different types of motivation: sexual motivation toward the opposite sex and competitive motivation toward the same sex. The genetic/molecular mechanisms underlying how mate presence affects male competitive motivation in a triadic relationship has remained largely unknown. Here we showed that male medaka fish prominently exhibit mateguarding behavior. The presence of a female robustly triggers male-male competition for the female in a triadic relationship (2 males and 1 female). The male-male competition resulted in one male occupying a dominant position near the female while interfering with the other male's approach of the female. Paternity testing revealed that the dominant male had a significantly higher mating success rate than the other male in a triadic relationship. We next generated medaka mutants of arginine-vasotocin (avt) and its receptors (V1a1, V1a2) and revealed that two genes, avt and V1a2, are required for normal mate-guarding behavior. In addition, behavioral analysis of courtship behaviors in a dyadic relationship and aggressive behaviors within a male group revealed that avt mutant males displayed decreased sexual motivation but showed normal aggression. In contrast, heterozygote V1a2 mutant males displayed decreased aggression, but normal mate-guarding and courtship behavior. Thus, impaired mate-guarding in avt and V1a2 homozygote mutants may be due to the loss of sexual motivation toward the opposite sex, and not to the loss of competitive motivation toward rival males. The different behavioral phenotypes between avt, V1a2 heterozygote, and V1a2 homozygote mutants suggest that there are redundant systems to activate V1a2 and that endogenous ligands activating the receptor may differ according to the social context.

  23. An essential role of the arginine vasotocin system in mate-guarding behaviors in triadic relationships of medaka fish (Oryzias latipes). 国際誌 査読有り

    Saori Yokoi, Teruhiro Okuyama, Yasuhiro Kamei, Kiyoshi Naruse, Yoshihito Taniguchi, Satoshi Ansai, Masato Kinoshita, Larry J Young, Nobuaki Takemori, Takeo Kubo, Hideaki Takeuchi

    PLoS genetics 11 (2) e1005009-Takeuchi 2015年

    出版者・発行元:2

    DOI: 10.1371/journal.pgen.1005009  

    ISSN:1553-7390

    詳細を見る 詳細を閉じる

    To increase individual male fitness, males of various species remain near a (potential) mating partner and repel their rivals (mate-guarding). Mate-guarding is assumed to be mediated by two different types of motivation: sexual motivation toward the opposite sex and competitive motivation toward the same sex. The genetic/molecular mechanisms underlying how mate presence affects male competitive motivation in a triadic relationship has remained largely unknown. Here we showed that male medaka fish prominently exhibit mate-guarding behavior. The presence of a female robustly triggers male-male competition for the female in a triadic relationship (2 males and 1 female). The male-male competition resulted in one male occupying a dominant position near the female while interfering with the other male's approach of the female. Paternity testing revealed that the dominant male had a significantly higher mating success rate than the other male in a triadic relationship. We next generated medaka mutants of arginine-vasotocin (avt) and its receptors (V1a1, V1a2) and revealed that two genes, avt and V1a2, are required for normal mate-guarding behavior. In addition, behavioral analysis of courtship behaviors in a dyadic relationship and aggressive behaviors within a male group revealed that avt mutant males displayed decreased sexual motivation but showed normal aggression. In contrast, heterozygote V1a2 mutant males displayed decreased aggression, but normal mate-guarding and courtship behavior. Thus, impaired mate-guarding in avt and V1a2 homozygote mutants may be due to the loss of sexual motivation toward the opposite sex, and not to the loss of competitive motivation toward rival males. The different behavioral phenotypes between avt, V1a2 heterozygote, and V1a2 homozygote mutants suggest that there are redundant systems to activate V1a2 and that endogenous ligands activating the receptor may differ according to the social context.

  24. A neural mechanism underlying mating preferences for familiar individuals in medaka fish. 国際誌 査読有り

    Teruhiro Okuyama, Saori Yokoi, Hideki Abe, Yasuko Isoe, Yuji Suehiro, Haruka Imada, Minoru Tanaka, Takashi Kawasaki, Shunsuke Yuba, Yoshihito Taniguchi, Yasuhiro Kamei, Kataaki Okubo, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Yoshitaka Oka, Takeo Kubo, Hideaki Takeuchi

    Science (New York, N.Y.) 343 (6166) 91-4 2014年1月3日

    出版者・発行元:6166

    DOI: 10.1126/science.1244724  

    ISSN:0036-8075

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    Social familiarity affects mating preference among various vertebrates. Here, we show that visual contact of a potential mating partner before mating (visual familiarization) enhances female preference for the familiarized male, but not for an unfamiliarized male, in medaka fish. Terminal-nerve gonadotropin-releasing hormone 3 (TN-GnRH3) neurons, an extrahypothalamic neuromodulatory system, function as a gate for activating mating preferences based on familiarity. Basal levels of TN-GnRH3 neuronal activity suppress female receptivity for any male (default mode). Visual familiarization facilitates TN-GnRH3 neuron activity (preference mode), which correlates with female preference for the familiarized male. GnRH3 peptides, which are synthesized specifically in TN-GnRH3 neurons, are required for the mode-switching via self-facilitation. Our study demonstrates the central neural mechanisms underlying the regulation of medaka female mating preference based on visual social familiarity.

  25. Genetic control of startle behavior in medaka fish. 国際誌 査読有り

    Satomi Tsuboko, Tetsuaki Kimura, Minori Shinya, Yuji Suehiro, Teruhiro Okuyama, Atsuko Shimada, Hiroyuki Takeda, Kiyoshi Naruse, Takeo Kubo, Hideaki Takeuchi

    PloS one 9 (11) e112527 2014年

    出版者・発行元:11

    DOI: 10.1371/journal.pone.0112527  

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    Genetic polymorphisms are thought to generate intraspecific behavioral diversities, both within and among populations. The mechanisms underlying genetic control of behavioral properties, however, remain unclear in wild-type vertebrates, including humans. To explore this issue, we used diverse inbred strains of medaka fish (Oryzias latipes) established from the same and different local populations. Medaka exhibit a startle response to a visual stimulus (extinction of illumination) by rapidly bending their bodies (C-start) 20-ms after the stimulus presentation. We measured the rates of the response to repeated stimuli (1-s interval, 40 times) among four inbred strains, HNI-I, HNI-II, HO5, and Hd-rR-II1, and quantified two properties of the startle response: sensitivity (response rate to the first stimulus) and attenuation of the response probability with repeated stimulus presentation. Among the four strains, the greatest differences in these properties were detected between HNI-II and Hd-rR-II1. HNI-II exhibited high sensitivity (approximately 80%) and no attenuation, while Hd-rR-II1 exhibited low sensitivity (approximately 50%) and almost complete attenuation after only five stimulus presentations. Our findings suggested behavioral diversity of the startle response within a local population as well as among different populations. Linkage analysis with F2 progeny between HNI-II and Hd-rR-II1 detected quantitative trait loci (QTL) highly related to attenuation, but not to sensitivity, with a maximum logarithm of odds score of 11.82 on linkage group 16. The three genotypes (homozygous for HNI-II and Hd-rR-II1 alleles, and heterozygous) at the marker nearest the QTL correlated with attenuation. Our findings are the first to suggest that a single genomic region might be sufficient to generate individual differences in startle behavior between wild-type strains. Further identification of genetic polymorphisms that define the behavioral trait will contribute to our understanding of the neural mechanisms underlying behavioral diversity, allowing us to investigate the adaptive significance of intraspecific behavioral polymorphisms of the startle response.

  26. Correction: Novel Middle-Type Kenyon Cells in the Honeybee Brain Revealed by Area-Preferential Gene Expression Analysis. 査読有り

    Kaneko K, Ikeda T, Nagai M, Hori S, Umatani C, Tadano H, Ugajin A, Nakaoka T, Paul RK, Fujiyuki T, Shirai K, Kunieda T, Takeuchi H, Kubo T

    PloS one 8 2013年11月

    出版者・発行元:11

    DOI: 10.1371/annotation/1fa31a02-1b58-4361-98eb-5c213e5d5336  

  27. Correction: A New Data-Mining Method to Search for Behavioral Properties That Induce Alignment and Their Involvement in Social Learning in Medaka Fish (<i>Oryzias Latipes</i>). 査読有り

    Ochiai T, Suehiro Y, Nishinari K, Kubo T, Takeuchi H

    PloS one 8 2013年11月

    出版者・発行元:11

    DOI: 10.1371/annotation/a895afc8-446c-4e3e-a71b-52f2baa9df10  

  28. A New Data-Mining Method to Search for Behavioral Properties That Induce Alignment and Their Involvement in Social Learning in Medaka Fish (Oryzias Latipes) 査読有り

    Takashi Ochiai, Yuji Suehiro, Katsuhiro Nishinari, Takeo Kubo, Hideaki Takeuchi

    PLOS ONE 8 (9) e71685 2013年9月

    出版者・発行元:PUBLIC LIBRARY SCIENCE

    DOI: 10.1371/journal.pone.0071685  

    ISSN:1932-6203

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    Background: Coordinated movement in social animal groups via social learning facilitates foraging activity. Few studies have examined the behavioral cause-and-effect between group members that mediates this social learning. Methodology/Principal Findings: We first established a behavioral paradigm for visual food learning using medaka fish and demonstrated that a single fish can learn to associate a visual cue with a food reward. Grouped medaka fish (6 fish) learn to respond to the visual cue more rapidly than a single fish, indicating that medaka fish undergo social learning. We then established a data-mining method based on Kullback-Leibler divergence (KLD) to search for candidate behaviors that induce alignment and found that high-speed movement of a focal fish tended to induce alignment of the other members locally and transiently under free-swimming conditions without presentation of a visual cue. The high-speed movement of the informed and trained fish during visual cue presentation appeared to facilitate the alignment of naive fish in response to some visual cues, thereby mediating social learning. Compared with naive fish, the informed fish had a higher tendency to induce alignment of other naive fish under free-swimming conditions without visual cue presentation, suggesting the involvement of individual recognition in social learning. Conclusions/Significance: Behavioral cause-and-effect studies of the high-speed movement between fish group members will contribute to our understanding of the dynamics of social behaviors. The data-mining method used in the present study is a powerful method to search for candidates factors associated with inter-individual interactions using a dataset for time-series coordinate data of individuals.

  29. Novel Middle-Type Kenyon Cells in the Honeybee Brain Revealed by Area-Preferential Gene Expression Analysis 査読有り

    Kumi Kaneko, Tsubomi Ikeda, Mirai Nagai, Sayaka Hori, Chie Umatani, Hiroto Tadano, Atsushi Ugajin, Takayoshi Nakaoka, Rajib Kumar Paul, Tomoko Fujiyuki, Kenichi Shirai, Takekazu Kunieda, Hideaki Takeuchi, Takeo Kubo

    PLOS ONE 8 (8) e71732 2013年8月

    出版者・発行元:PUBLIC LIBRARY SCIENCE

    DOI: 10.1371/journal.pone.0071732  

    ISSN:1932-6203

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    The mushroom bodies (a higher center) of the honeybee (Apis mellifera L) brain were considered to comprise three types of intrinsic neurons, including large- and small-type Kenyon cells that have distinct gene expression profiles. Although previous neural activity mapping using the immediate early gene kakusei suggested that small-type Kenyon cells are mainly active in forager brains, the precise Kenyon cell types that are active in the forager brain remain to be elucidated. We searched for novel gene(s) that are expressed in an area-preferential manner in the honeybee brain. By identifying and analyzing expression of a gene that we termed mKast (middle-type Kenyon cell-preferential arrestin-related protein), we discovered novel 'middle-type Kenyon cells' that are sandwiched between large- and small-type Kenyon cells and have a gene expression profile almost complementary to those of large- and small-type Kenyon cells. Expression analysis of kakusei revealed that both small-type Kenyon cells and some middle-type Kenyon cells are active in the forager brains, suggesting their possible involvement in information processing during the foraging flight. mKast expression began after the differentiation of small- and large-type Kenyon cells during metamorphosis, suggesting that middle-type Kenyon cells differentiate by modifying some characteristics of large- and/or small-type Kenyon cells. Interestingly, CaMKII and mKast, marker genes for large- and middle-type Kenyon cells, respectively, were preferentially expressed in a distinct set of optic lobe (a visual center) neurons. Our findings suggested that it is not simply the Kenyon cell-preferential gene expression profiles, rather, a 'clustering' of neurons with similar gene expression profiles as particular Kenyon cell types that characterize the honeybee mushroom body structure.

  30. Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes. 国際誌 査読有り

    Teruhiro Okuyama, Yasuko Isoe, Masahito Hoki, Yuji Suehiro, Genki Yamagishi, Kiyoshi Naruse, Masato Kinoshita, Yasuhiro Kamei, Atushi Shimizu, Takeo Kubo, Hideaki Takeuchi

    PloS one 8 (6) e66597 2013年

    出版者・発行元:6

    DOI: 10.1371/journal.pone.0066597  

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    BACKGROUND: Genetic mosaic techniques have been used to visualize and/or genetically modify a neuronal subpopulation within complex neural circuits in various animals. Neural populations available for mosaic analysis, however, are limited in the vertebrate brain. METHODOLOGY/PRINCIPAL FINDINGS: To establish methodology to genetically manipulate neural circuits in medaka, we first created two transgenic (Tg) medaka lines, Tg (HSP:Cre) and Tg (HuC:loxP-DsRed-loxP-GFP). We confirmed medaka HuC promoter-derived expression of the reporter gene in juvenile medaka whole brain, and in neuronal precursor cells in the adult brain. We then demonstrated that stochastic recombination can be induced by micro-injection of Cre mRNA into Tg (HuC:loxP-DsRed-loxP-GFP) embryos at the 1-cell stage, which allowed us to visualize some subpopulations of GFP-positive cells in compartmentalized regions of the telencephalon in the adult medaka brain. This finding suggested that the distribution of clonally-related cells derived from single or a few progenitor cells was restricted to a compartmentalized region. Heat treatment of Tg(HSP:Cre x HuC:loxP-DsRed-loxP-GFP) embryos (0-1 day post fertilization [dpf]) in a thermalcycler (39°C) led to Cre/loxP recombination in the whole brain. The recombination efficiency was notably low when using 2-3 dpf embyos compared with 0-1 dpf embryos, indicating the possibility of stage-dependent sensitivity of heat-inducible recombination. Finally, using an infrared laser-evoked gene operator (IR-LEGO) system, heat shock induced in a micro area in the developing brains led to visualization of clonally-related cells in both juvenile and adult medaka fish. CONCLUSIONS/SIGNIFICANCE: We established a noninvasive method to control Cre/loxP site-specific recombination in the developing nervous system in medaka fish. This method will broaden the neural population available for mosaic analyses and allow for lineage tracing of the vertebrate nervous system in both juvenile and adult stages.

  31. p53 Mutation suppresses adult neurogenesis in medaka fish (Oryzias latipes). 国際誌 査読有り

    Yasuko Isoe, Teruhiro Okuyama, Yoshihito Taniguchi, Takeo Kubo, Hideaki Takeuchi

    Biochemical and biophysical research communications 423 (4) 627-31 2012年7月13日

    DOI: 10.1016/j.bbrc.2012.05.125  

    ISSN:0006-291X

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    Tumor suppressor p53 negatively regulates self-renewal of neural stem cells in the adult murine brain. Here, we report that the p53 null mutation in medaka fish (Oryzias latipes) suppressed neurogenesis in the telencephalon, independent of cell death. By using 5-bromo-29-deoxyuridine (BrdU) immunohistochemistry, we identified 18 proliferation zones in the brains of young medaka fish; in situ hybridization showed that p53 was expressed selectively in at least 12 proliferation zones. We also compared the number of BrdU-positive cells present in the whole telencephalon of wild-type (WT) and p53 mutant fish. Immediately after BrdU exposure, the number of BrdU-positive cells did not differ significantly between them. One week after BrdU-exposure, the BrdU-positive cells migrated from the proliferation zone, which was accompanied by an increased number in the WT brain. In contrast, no significant increase was observed in the p53 mutant brain. Terminal deoxynucleotidyl transferase (dUTP) nick end-labeling revealed that there was no significant difference in the number of apoptotic cells in the telencephalon of p53 mutant and WT medaka, suggesting that the decreased number of BrdU-positive cells in the mutant may be due to the suppression of proliferation rather than the enhancement of neural cell death. These results suggest that p53 positively regulates neurogenesis via cell proliferation.

  32. Neural mechanisms of female sexual preference in medaka 査読有り

    Okuyama T, Yokoi S, Abe H, Taniguchi Y, Okubo K, Kamei Y, Shimada A, Naruse K, Takeda H, Oka Y, Kubo T, Takeuchi H

    Society for Neuroscience Abstract Viewer and Itinerary Planner 42 2012年

  33. Ecdysteroid biosynthesis in workers of the European honeybee Apis mellifera L. 査読有り

    Yurika Yamazaki, Makoto Kiuchi, Hideaki Takeuchi, Takeo Kubo

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 41 (5) 283-293 2011年5月

    出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD

    DOI: 10.1016/j.ibmb.2011.01.005  

    ISSN:0965-1748

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    We previously reported preferential expression of genes for ecdysteroid signaling in the mushroom bodies of honeybee workers, suggesting a role of ecdysteroid signaling in regulating honeybee behaviors. The organs that produce ecdysteroids in worker honeybees, however, remain unknown. We show here that the expression of neverland and Non-molting glossy/shroud, which are involved in early steps of ecdysteroid synthesis, was enhanced in the ovary, while the expression of CYP306A1 and CYP302A1, which are involved in later steps of ecdysone synthesis, was enhanced in the brain, and the expression of CYP314A1, which is involved in converting ecdysone into active 20-hydroxyecdysone (20E), was enhanced in the brain, fat body, and ovary. In in vitro organ culture, a significant amount of ecdysteroids was detected in the culture medium of the brain, fat body, and hypopharyngeal glands. The ecdysteroids detected in the culture medium of the fat body were identified as ecdysone and 20E. These findings suggest that, in worker honeybees, cholesterol is converted into intermediate ecdysteroids in the ovary, whereas ecdysone is synthesized and secreted mainly by the brain and converted into 20E in the brain and fat body. (C) 2011 Elsevier Ltd. All rights reserved.

  34. Induction of c-fos transcription in the medaka brain (Oryzias latipes) in response to mating stimuli. 国際誌 査読有り

    Teruhiro Okuyama, Yuji Suehiro, Haruka Imada, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    Biochemical and biophysical research communications 404 (1) 453-7 2011年1月7日

    出版者・発行元:1

    DOI: 10.1016/j.bbrc.2010.11.143  

    ISSN:0006-291X

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    Immediate-early genes (IEGs) are useful for mapping active brain regions in various vertebrates. Here we identified a c-fos homologue gene in medaka and demonstrated that the amounts of c-fos transcripts and proteins in the medaka brain increased in relation to an artificially evoked seizure, suggesting that the homologue gene has the characteristics of IEGs, which are used as markers of neural activity. Next, quantitative reverse-transcription-polymerase chain reaction revealed that female mating behaviors upregulated c-fos transcription in some brain regions including the telencephalon, optic tectum, and cerebellum. In addition, we performed in situ hybridization with a c-fos intron probe to detect the de novo synthesis of c-fos transcripts and confirmed induction of c-fos transcription in these brain regions after mating. This is the first report of IEG induction in response to mating stimuli in teleost fish. Our results indicated that c-fos expression was induced in response to behavioral stimuli in the medaka brain and that medaka c-fos could be a useful marker of neural activity.

  35. Neural mechanism for female mating preference of medaka mediated by visual information 査読有り

    Teruhiro Okuyama, Yuji Suehiro, Haruka Imada, Hideki Abe, Atsuko Shimada, Minoru Tanaka, Kiyoshi Naruse, Hiroyuki Takeda, Yoshitaka Oka, Takeo Kubo, Hideaki Takeuchi

    NEUROSCIENCE RESEARCH 71 E267-E267 2011年

    出版者・発行元:ELSEVIER IRELAND LTD

    DOI: 10.1016/j.neures.2011.07.1166  

    ISSN:0168-0102

  36. Analysis of the molecular and neural mechanism of female mating acceptance depending on visual information in small fish medaka 査読有り

    Okuyama T, Suehiro Y, Imada H, Shimada A, Abe H, Tanaka M, Naruse K, Takeda H, Oka Y, Kubo T, Takeuchi H

    Society for Neuroscience Abstract Viewer and Itinerary Planner 41 2011年

  37. Expression of two microRNAs, ame-mir-276 and-1000, in the adult honeybee (Apis mellifera) brain 査読有り

    Sayaka Hori, Kumi Kaneko, Takeshi H. Saito, Hideaki Takeuchi, Takeo Kubo

    APIDOLOGIE 42 (1) 89-102 2011年1月

    出版者・発行元:SPRINGER FRANCE

    DOI: 10.1051/apido/2010032  

    ISSN:0044-8435

    eISSN:1297-9678

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    To identify candidate microRNAs involved in post-transcriptional regulation of brain (region)selective gene expression in the adult honeybee brain, we isolated eight microRNAs: seven known microRNAs, ame-mir-2-1, -8, 13a, -34, -276, -317, -1000, and one novel one, named mir-hbd, that has significant sequence similarity with the Drosophila dme-mir-11. Among them, ame-mir-1000 and -276 were expressed in a brain-selective and -preferential manner, respectively, in workers and drones. In particular, ame-mir-276-expression was enriched in the optic lobes and in the small type-Kenyon cells of the mushroom bodies in the nurse bee, forager, queen, and drone brains. Almost all predicted targets of ame-mir-1000 and -276 encode neural function related genes, suggesting the involvement in neural function of both microRNAs.

  38. Transient and permanent gene transfer into the brain of the teleost fish medaka (Oryzias latipes) using human adenovirus and the Cre-loxP system. 国際誌 査読有り

    Yuji Suehiro, Masato Kinoshita, Teruhiro Okuyama, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Mitsuhiro Hashimoto, Hideaki Takeuchi

    FEBS letters 584 (16) 3545-9 2010年8月20日

    DOI: 10.1016/j.febslet.2010.06.047  

    ISSN:0014-5793

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    In this study, we demonstrated that human type-5 adenovirus infected the brain of the teleost fish, medaka (Oryzias latipes), in vivo. Injection of adenoviral vector into the mesencephalic ventricle of medaka larvae induced the expression of reporter genes in some parts of the telencephalon, the periventricular area of the mesencephalon and diencephalon, and the cerebellum. Additionally, the Cre-loxP system works in medaka brains using transgenic medaka carrying a vector containing DsRed2, flanked by loxP sites under control of the beta-actin promoter and downstream promoterless enhanced green fluorescent protein (EGFP). We demonstrated that the presence of green fluorescence depended on injection of adenoviral vector expressing the Cre gene and confirmed that EGFP mRNA was transcribed in the virus-injected larvae.

  39. Coordinated and cohesive movement of two small conspecific fish induced by eliciting a simultaneous optomotor response. 国際誌 査読有り

    Haruka Imada, Masahito Hoki, Yuji Suehiro, Teruhiro Okuyama, Daisuke Kurabayashi, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    PloS one 5 (6) e11248 2010年6月22日

    DOI: 10.1371/journal.pone.0011248  

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    BACKGROUND: In animal groups such as herds, schools, and flocks, a certain distance is maintained between adjacent individuals, allowing them to move as a cohesive unit. Proximate causations of the cohesive and coordinated movement under dynamic conditions, however, have been poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: We established a novel and simple behavioral assay using pairs of small fish (medaka and dwarf pufferfish) by eliciting a simultaneous optomotor response (OMR). We demonstrated that two homospecific fish began to move cohesively and maintained a distance of 2 to 4 cm between them when an OMR was elicited simultaneously in the fish. The coordinated and cohesive movement was not exhibited under a static condition. During the cohesive movement, the relative position of the two fish was not stable. Furthermore, adult medaka exhibited the cohesive movement but larvae did not, despite the fact that an OMR could be elicited in larvae, indicating that this ability to coordinate movement develops during maturation. The cohesive movement was detected in homospecific pairs irrespective of body-color, sex, or albino mutation, but was not detected between heterospecific pairs, suggesting that coordinated movement is based on a conspecific interaction. CONCLUSIONS/SIGNIFICANCE: Our findings demonstrate that coordinated behavior between a pair of animals was elicited by a simultaneous OMR in two small fish. This is the first report to demonstrate induction of a schooling-like movement in a pair of fish by an OMR and to investigate the effect of age, sex, body color, and species on coordination between animals under a dynamic condition.

  40. Proliferation zones in adult medaka (Oryzias latipes) brain. 国際誌 査読有り

    Yoshiko Kuroyanagi, Teruhiro Okuyama, Yuji Suehiro, Haruka Imada, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    Brain research 1323 33-40 2010年4月6日

    DOI: 10.1016/j.brainres.2010.01.045  

    ISSN:0006-8993

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    Cell proliferation in the adult mammalian brain is maintained at a low rate, but cell proliferation in the adult fish brain is prominent. To compare the distribution of proliferating cells among fish species, mutants, and under different growing environments, we mapped the zones of cell proliferation in the adult medaka (Oryzias latipes) brain and identified 17 proliferation zones in both male and female brains. These zones were distributed in the telencephalon (4 zones), preoptic area (2 zones), pineal body (1 zone), hypophysis (1 zone), habenular nucleus (1 zone), optic tectum (2 zones), third ventricular zone (1 zone), ventromedial nucleus (1 zone), hypothalamus (1 zone), and cerebellum (3 zones). Of the 17 zones, 16 corresponded to brain regions where cells proliferate in the zebrafish brain, suggesting that the persistence of the generation of new cells, at least in these zones, might be conserved among some fish species. We then compared the distribution of proliferation zones using two body-color mutant medaka, the T5 and Quintet, the latter of which is an albino mutant that completely lacks pigmentation. There was no apparent difference in the distribution pattern among these mutant strains. Finally, we compared these proliferation zones in the brains of isolated- and group-reared fish and detected no significant difference between the two groups. These findings demonstrate that there is persistent cell proliferation in at least these 16 zones of the adult medaka brain, irrespective of sex, body-color, and growth environment, suggesting that proliferation capacity in the 16 zones is maintained robustly in the adult medaka brain.

  41. Structural diversity and evolution of the N-terminal isoform-specific region of ecdysone receptor-A and-B1 isoforms in insects 査読有り

    Takayuki Watanabe, Hideaki Takeuchi, Takeo Kubo

    BMC EVOLUTIONARY BIOLOGY 10 40 2010年2月

    出版者・発行元:BIOMED CENTRAL LTD

    DOI: 10.1186/1471-2148-10-40  

    ISSN:1471-2148

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    Background: The ecdysone receptor (EcR) regulates various cellular responses to ecdysteroids during insect development. Insects have multiple EcR isoforms with different N-terminal A/B domains that contain the isoform-specific activation function (AF)-1 region. Although distinct physiologic functions of the EcR isoforms have been characterized in higher holometabolous insects, they remain unclear in basal direct-developing insects, in which only A isoform has been identified. To examine the structural basis of the EcR isoform-specific AF-1 regions, we performed a comprehensive structural comparison of the isoform-specific region of the EcR-A and -B1 isoforms in insects. Results: The EcR isoforms were newly identified in 51 species of insects and non-insect arthropods, including direct-developing ametabolous and hemimetabolous insects. The comprehensive structural comparison revealed that the isoform-specific region of each EcR isoform contained evolutionally conserved microdomain structures and insect subgroup-specific structural modifications. The A isoform-specific region generally contained four conserved microdomains, including the SUMOylation motif and the nuclear localization signal, whereas the B1 isoform-specific region contained three conserved microdomains, including an acidic activator domain-like motif. In addition, the EcR-B1 isoform of holometabolous insects had a novel microdomain at the N-terminal end. Conclusions: Given that the nuclear receptor AF-1 is involved in cofactor recruitment and transcriptional regulation, the microdomain structures identified in the isoform-specific A/B domains might function as signature motifs and/or as targets for cofactor proteins that play essential roles in the EcR isoform-specific AF-1 regions. Moreover, the novel microdomain in the isoform-specific region of the holometabolous insect EcR-B1 isoform suggests that the holometabolous insect EcR-B1 acquired additional transcriptional regulation mechanisms.

  42. In Situ Hybridization Analysis of the Expression of Futsch, Tau, and MESK2 Homologues in the Brain of the European Honeybee (Apis mellifera L.) 査読有り

    Kumi Kaneko, Sayaka Hori, Mai M. Morimoto, Takayoshi Nakaoka, Rajib Kumar Paul, Tomoko Fujiyuki, Kenichi Shirai, Akiko Wakamoto, Satomi Tsuboko, Hideaki Takeuchi, Takeo Kubo

    PLOS ONE 5 (2) e9213 2010年2月

    出版者・発行元:PUBLIC LIBRARY SCIENCE

    DOI: 10.1371/journal.pone.0009213  

    ISSN:1932-6203

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    Background: The importance of visual sense in Hymenopteran social behavior is suggested by the existence of a Hymenopteran insect-specific neural circuit related to visual processing and the fact that worker honeybee brain changes morphologically according to its foraging experience. To analyze molecular and neural bases that underlie the visual abilities of the honeybees, we used a cDNA microarray to search for gene(s) expressed in a neural cell-type preferential manner in a visual center of the honeybee brain, the optic lobes (OLs). Methodology/Principal Findings: Expression analysis of candidate genes using in situ hybridization revealed two genes expressed in a neural cell-type preferential manner in the OLs. One is a homologue of Drosophila futsch, which encodes a microtubule-associated protein and is preferentially expressed in the monopolar cells in the lamina of the OLs. The gene for another microtubule-associated protein, tau, which functionally overlaps with futsch, was also preferentially expressed in the monopolar cells, strongly suggesting the functional importance of these two microtubule-associated proteins in monopolar cells. The other gene encoded a homologue of Misexpression Suppressor of Dominant-negative Kinase Suppressor of Ras 2 (MESK2), which might activate Ras/MAPK-signaling in Drosophila. MESK2 was expressed preferentially in a subclass of neurons located in the ventral region between the lamina and medulla neuropil in the OLs, suggesting that this subclass is a novel OL neuron type characterized by MESK2-expression. These three genes exhibited similar expression patterns in the worker, drone, and queen brains, suggesting that they function similarly irrespective of the honeybee sex or caste. Conclusions: Here we identified genes that are expressed in a monopolar cell (Amfutsch and Amtau) or ventral medulla-preferential manner (AmMESK2) in insect OLs. These genes may aid in visualizing neurites of monopolar cells and ventral medulla cells, as well as in analyzing the function of these neurons.

  43. Age- and division-of-labour-dependent differential expression of a novel non-coding RNA, Nb-1, in the brain of worker honeybees, Apis mellifera L. 査読有り

    H. Tadano, Y. Yamazaki, H. Takeuchi, T. Kubo

    INSECT MOLECULAR BIOLOGY 18 (6) 715-726 2009年12月

    出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC

    DOI: 10.1111/j.1365-2583.2009.00911.x  

    ISSN:0962-1075

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    To elucidate the molecular mechanisms underlying honeybee social behaviours, we identified a novel gene, Nb-1, whose expression in the worker brain changes according to the age-dependent division of labour in normal colonies. The open reading frames contained in the Nb-1 cDNA were not conserved in the homologue of a related species, suggesting that the Nb-1 gene product is a non-coding RNA. The distribution of Nb-1-expressing cells partially overlapped that of octopamine-immunoreactive cells and neurosecretory cells, the latter of which are involved in the synthesis and secretion of juvenile hormone (JH). Octopamine and JH control worker task transition, and thus Nb-1 might be involved in task transition through the modulation of octopamine/JH synthesis and secretion.

  44. Distribution of Kakugo Virus and Its Effects on the Gene Expression Profile in the Brain of the Worker Honeybee Apis mellifera L. 査読有り

    Tomoko Fujiyuki, Emiko Matsuzaka, Takayoshi Nakaoka, Hideaki Takeuchi, Akiko Wakamoto, Seii Ohka, Kazuhisa Sekimizu, Akio Nomoto, Takeo Kubo

    JOURNAL OF VIROLOGY 83 (22) 11560-11568 2009年11月

    出版者・発行元:AMER SOC MICROBIOLOGY

    DOI: 10.1128/JVI.00519-09  

    ISSN:0022-538X

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    We previously identified a novel insect picorna-like virus, termed Kakugo virus (KV), obtained from the brains of aggressive honeybee worker bees that had counterattacked giant hornets. Here we examined the tissue distribution of KV and alterations of gene expression profiles in the brains of KV-infected worker bees to analyze possible effects of KV infection on honeybee neural and physiological states. By use of in situ hybridization, KV was broadly detected in the brains of the naturally KV-infected worker bees. When inoculated experimentally into bees, KV was detected in restricted parts of the brain at the early infectious stage and was later detected in various brain regions, including the mushroom bodies, optic lobes, and ocellar nerve. KV was detected not only in the brain but also in the hypopharyngeal glands and fat bodies, indicating systemic KV infection. Next, we compared the gene expression profiles in the brains of KV-inoculated and noninoculated bees. The expression of 11 genes examined was not significantly affected in KV-infected worker bees. cDNA microarray analysis, however, identified a novel gene whose expression was induced in the periphery of the brains of KV-infected bees, which was commonly observed in naturally infected and experimentally inoculated bees. The gene encoded a novel hypothetical protein with a leucine zipper motif. A gene encoding a similar protein was found in the parasitic wasp Nasonia genome but not in other insect genomes. These findings suggest that KV infection may affect brain functions and/or physiological states in honeybees.

  45. Differential Gene Expression in the Hypopharyngeal Glands of Worker Honeybees (Apis mellifera L.) Associated with an Age-Dependent Role Change 査読有り

    Takayuki Ueno, Takayoshi Nakaoka, Hideaki Takeuchi, Takeo Kubo

    ZOOLOGICAL SCIENCE 26 (8) 557-563 2009年8月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    DOI: 10.2108/zsj.26.557  

    ISSN:0289-0003

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    Associated with the age-dependent role change of worker honeybees (Apis mellifera L.) from nurse bees to foragers, some structural and functional changes occur in the hypopharyngeal glands (HPGs): nurse bee HPGs are well developed and synthesize major royal jelly proteins (MRJPs), while forager HPGs shrink and synthesize alpha-glucosidase, which converts nectar into honey. To identify candidate genes involved in the structural and functional HPG changes associated with the age-dependent role change of worker honeybees, we searched for genes whose expression in the HPGs depends on the role of workers, by using differential display and quantitative reverse transcription-polymerase chain reaction. Here, we newly identified a buffy homolog encoding a Bcl-2-like protein as a gene whose expression, like MRJP, is higher in nurse bees than in foragers, and a matrix metalloproteinase 1 (MMP1) homolog as a gene whose expression, like alpha-glucosidase, is higher in foragers than in nurse bees, suggesting that both suppression of inhibition of the caspase cascade by buffy and degradation of the extracellular matrix by MMP1 are involved in the functional and structural changes of the HPGs. Furthermore, although both buffy and MMP1 were highly expressed in various tissues other than the HPGs, buffy expression in the other tissues did not differ significantly between nurse bees and foragers, whereas MMP1 expression in midgut was also significantly higher in foragers than in nurse bees, as in the HPGs. These results suggest that in buffy and MMP1, expression is regulated in a tissue-preferential manner according to the age-dependent role change of workers.

  46. Mass spectrometric map of neuropeptide expression and analysis of the gamma-prepro-tachykinin gene expression in the medaka (Oryzias latipes) brain. 国際誌 査読有り

    Yuji Suehiro, Akikazu Yasuda, Teruhiro Okuyama, Haruka Imada, Yoshiko Kuroyanagi, Takeo Kubo, Hideaki Takeuchi

    General and comparative endocrinology 161 (1) 138-45 2009年3月

    出版者・発行元:1

    DOI: 10.1016/j.ygcen.2008.12.001  

    ISSN:0016-6480

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    Neuropeptides have important roles in modulating behavioral patterns such as social interaction. With the aim to determine the presence of neuropeptides known to be involved in social interaction as well as novel peptides, we used MALDI-TOF/MS to analyze neuropeptide profiles in some medaka brain regions. In the telencephalon, hypothalamus, and pituitary gland, 3, 6, and 10 peaks, respectively, were identified as neuropeptides (Arg-vasotocin [AVT], growth hormone-releasing hormone [GHRH], neuropeptide FF, substance P [SP], somatostatin-1 and -2, melanin-concentrating hormone [MCH], MCH gene-related peptide [Mgrp], melanocyte-stimulating hormone [MSH], corticotropin-like intermediate lobe peptide [CLIP], and beta-endorphin). The neuropeptide profile of telencephalon similar to that of the hypothalamus, but completely different from that of pituitary gland. For the future genetic analysis, we identified cDNAs encoding precursor proteins for the identified peptides. We also detect its expression of gamma-prepro-tachykinin gene encoding a SP precursor protein in both the telencephalon and hypothalamus. Our results indicated that the medaka brain contains some neuropeptides (AVT, SP, and somatostatins) that may be involved in modulating medaka behaviors such as social interaction.

  47. [New approach toward understanding of neural basis of the honeybee "dance communication"]. 査読有り

    Kiya T, Hori S, Takeuchi H, Kubo T

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 53 (11) 1368-1374 2008年9月

    出版者・発行元:11

    ISSN:0039-9450

  48. Gene expression profile in cerebrum in the filial imprinting of domestic chicks (Gallus gallus domesticus) 査読有り

    Shinji Yamaguchi, Ikuko Fujii-Taira, Sachiko Katagiri, Ei-Ichi Izawa, Yasuyuki Fujimoto, Hideaki Takeuchi, Tatsuya Takano, Toshiya Matsushima, Koichi J. Homma

    BRAIN RESEARCH BULLETIN 76 (3) 275-281 2008年6月

    出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD

    DOI: 10.1016/j.brainresbull.2008.02.002  

    ISSN:0361-9230

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    In newly hatched chicks, gene expression in the brain has previously been shown to be up-regulated following filial imprinting. By applying cDNA rnicroarrays containing 13,007 expressed sequence tags, we examined the comprehensive gene expression profiling of the intermediate medial mesopallium in the chick cerebrum, which has been shown to play a key role in filial imprinting. We found 52 up-regulated genes and 6 down-regulated genes of at least 2.0-fold changes 3 h after the training of filial imprinting, compared to the gene expression of the dark-reared chick brain. The up-regulated genes are known to be involved in a variety of pathways, including signal transduction, cytoskeletal organization, nuclear function, cell metabolism, RNA binding, endoplasmic reticulum or Golgi function, synaptic function, ion channel, and transporter. In contrast, fewer genes were down-regulated in the imprinting, coinciding with the previous data that the total RNA synthesis increased associated with filial imprinting. Our data suggests that the filial imprinting involves the modulation of multiple signaling pathways. (C) 2008 Elsevier Inc. All rights reserved.

  49. Laying workers in queenless honeybee (Apis mellifera L.) colonies have physiological states similar to that of nurse bees but opposite that of foragers 査読有り

    Takayoshi Nakaoka, Hideaki Takeuchi, Takeo Kubo

    JOURNAL OF INSECT PHYSIOLOGY 54 (5) 806-812 2008年5月

    出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD

    DOI: 10.1016/j.jinsphys.2008.02.007  

    ISSN:0022-1910

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    Honeybee workers shift their labors from nursing their brood to foraging according to their age after eclosion. When the queen is lost from the colony, however, some workers become 'laying workers' whose ovaries develop to lay eggs. Here we investigated whether the physiological state of laying workers is more similar to that of nurse bees or foragers by examining the hypopharyngeal gland (HPG) and hemolymph vitellogenin titers. In a normal colony, nurse bees have well-developed HPGs that synthesize 'major royal jelly proteins' and high hemolymph vitellogenin, titers, whereas foragers have shrunken HPGs that synthesize 70-kDa alpha-glucosidase and low hemolymph vitellogenin titers. In queenless colonies, however, workers with developed ovaries (laying workers) tended to have more developed HPGs and to synthesize major royal jelly proteins, whereas workers with shrunken HPGs tended to synthesize alpha-glucosidase and to have undeveloped ovaries. Furthermore, the workers with developed ovaries had higher vitellogenin titers than nurse bees, whereas those with undeveloped ovaries had lower vitellogenin titers. These findings indicate that the physiological state of laying workers is similar to that of nurse bees, but opposite that of foragers. (C) 2008 Elsevier Ltd. All rights reserved.

  50. メダカの視運動反応の情報処理過程とその神経基盤の解析

    末廣勇司, 奥山輝大, 今田はるか, 成瀬清, 島田敦子, 武田洋幸, 久保健雄, 竹内秀明

    第18回 インテリジェントシステムシンポジウム講演論文集 501-504 2008年

  51. Associative learning and discrimination of motion cues in the harnessed honeybee Apis mellifera L. 査読有り

    Sayaka Hori, Hideaki Takeuchi, Takeo Kubo

    JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY 193 (8) 825-833 2007年8月

    出版者・発行元:SPRINGER

    DOI: 10.1007/s00359-007-0234-x  

    ISSN:0340-7594

    eISSN:1432-1351

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    We previously studied a conditioning paradigm to associate the proboscis extension reflex (PER) with monochromatic light (conditioned stimulus; CS) in harnessed honeybees. Here, we established a novel conditioning paradigm to associate the PER with a motion cue generated using graphics interchange format (GIF) animations with a speed of 12 mm/s speed and a frame rate of 25 Hz as the CS, which were projected onto a screen consisting of a translucent circular cone that largely covered the visual field of the harnessed bee using two liquid crystal projectors. The acquisition rate reached a plateau at approximately 40% after seven trials, indicating that the bees were successfully conditioned with the motion cue. We demonstrated four properties of the conditioning paradigm. First, the acquisition rate was enhanced by antennae deprivation, suggesting that sensory input from the antennae interferes with the visual associative learning. Second, bees conditioned with a backward-direction motion cue did not respond to the forward-direction, suggesting that bees can discriminate the two directions in this paradigm. Third, the bees can retain memory for motion cue direction for 48 h. Finally, the acquisition rate did not differ significantly between foragers and nurse bees.

  52. EcR-A expression in the brain and ovary of the honeybee (Apis mellifera L.) 査読有り

    Hideaki Takeuchi, Rajib Kumar Paul, Emiko Matsuzaka, Takeo Kubo

    ZOOLOGICAL SCIENCE 24 (6) 596-603 2007年6月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    DOI: 10.2108/zsj.24.596  

    ISSN:0289-0003

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    We previously demonstrated that six genes involved in ecdysteroid signaling are expressed preferentially in Kenyon-cell subtypes in the mushroom bodies of the honeybee (Apis mellifera L.). To further examine the possible involvement of ecdysteroid signaling in honeybee brain function, we isolated a cDNA for the A isoform of the ecdysone receptor gene homolog AmEcR-A and analyzed its expression in the brain. In situ hybridization revealed that AmEcR-A is expressed selectively in the small-type Kenyon cells of the mushroom bodies in the worker and queen brain, like AmE74 and AmHR38, suggesting a possible association of these gene products. Analysis of AmEcR-A expression in queen and worker abdomens demonstrated that AmEcR-A is strongly expressed in nurse cells of the queen ovary, suggesting that ecdysteroid and ecdysteroid signaling have roles in oogenesis. Our present results further support the possible involvement of ecdysterold signaling in brain function, as well as in regulating queen reproductive physiology in the adult honeybee.

  53. Gene expression in the honeybee mushroom body and its gene orthologues

    Takeuchi, H.

    Evolution of Nervous Systems 1 2007年

    DOI: 10.1016/B0-12-370878-8/00188-9  

  54. Identification of proteins whose expression is up- or down-regulated in the mushroom bodies in the honeybee brain using proteomics 査読有り

    Yuko Uno, Tomoko Fujiyuki, Mizue Morioka, Hideaki Takeuchi, Takeo Kubo

    FEBS LETTERS 581 (1) 97-101 2007年1月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/j.febslet.2006.12.004  

    ISSN:0014-5793

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    To identify protein(s) with different expression patterns in the mushroom bodies (MBs) in the honeybee brain, we compared the protein profiles of MBs and optic lobes (OLs) using proteomics. Two-dimensional gel electrophoresis revealed that five and three spots were selectively expressed in the MBs or OLs, respectively. Liquid chromatography tandem mass spectrometry analysis identified juvenile hormone diol kinase and glyceraidehyde-3-phosphate dehydrogenase as MB- and OL-selective proteins, respectively. In situ hybridization revealed that jhdk expression was upregulated in MB neuron subsets, whereas gapdh expression was downregulated, indicating that MBs have a distinct gene and protein expression profile in the honeybee brain. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  55. Expression of two ecdysteroid-regulated genes, Broad-Complex and E75, in the brain and ovary of the honeybee (Apis mellifera L.) 査読有り

    Rajib Kumar Paul, Hideaki Takeuchi, Takeo Kubo

    ZOOLOGICAL SCIENCE 23 (12) 1085-1092 2006年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    DOI: 10.2108/zsj.23.1085  

    ISSN:0289-0003

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    We previously demonstrated that two ecdysteroid-regulated genes, MbIk-11E93 and E74, are expressed selectively in Kenyon cell subtypes in the mushroom bodies of the honeybee (Apis mellifera L.) brain. To further examine the possible involvement of ecdysteroid-regulated genes in brain function as well as in oogenesis in the honeybee, we isolated cDNAs for two other ecdysteroid-regulated genes, Broad-Complex (BR-C) and E75, and analyzed their expression in the worker brain as well as in the queen abdomen. In situ hybridization revealed that BR-C, like Mblk-1/E93, is expressed selectively in the large-type Kenyon cells of the mushroom bodies in the worker brain, whereas E75 is expressed in all mushroom body neuron subtypes, suggesting a difference in the mode of response to ecdysteroid among Kenyon cell subtypes. In the queen ovary, both BR-C and E75 are expressed preferentially in the follicle cells that surround egg cells at the late stage, suggesting their role in oogenesis. These results suggest that BR-C and E75 are involved in the regulation of brain function as well as in reproductive physiology in the adult honeybee.

  56. Prevalence and phylogeny of Kakugo virus, a novel insect picorna-like virus that infects the honeybee (Apis mellifera L.), under various colony conditions 査読有り

    Tornoko Fujiyuki, Seii Ohka, Hideaki Takeuchi, Masato Ono, Akio Nomoto, Takeo Kubo

    JOURNAL OF VIROLOGY 80 (23) 11528-11538 2006年12月

    出版者・発行元:AMER SOC MICROBIOLOGY

    DOI: 10.1128/JVI.00754-06  

    ISSN:0022-538X

    eISSN:1098-5514

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    We previously identified a novel insect picorna-like virus, termed Kakugo virus (K-V), from the brains of aggressive worker honeybees that had counterattacked a giant hornet. To survey the prevalence of KV in worker populations engaged in various labors, we quantified KV genomic RNA. KV was detected specifically from aggressive workers in some colonies, while it was also detected from other worker populations in other colonies where the amount of KV detected in the workers was relatively high, suggesting that KV can infect various worker populations in the honeybee colonies. To investigate whether the KV strains detected were identical, phylogenetic analysis was performed. There was less than a 2% difference in the RNA-dependent RNA polymerase (RdRp) sequences between KV strains from aggressive workers and those from other worker populations, suggesting that all of the viruses detected were virtually the same KV. We also found that some of the K-V-infected colonies were parasitized by Varroa mites, and the sequences of the KV strains detected from the mites were the same as those detected from the workers of the same colonies, suggesting that the mites mediate KV prevalence in the honeybee colonies. KV strains had approximately 6% and 15% sequence differences in the RdRp region from deformed wing virus and Varroa destructor virus 1, respectively, suggesting that KV represents a viral strain closely related to, but distinct from, these two viruses.

  57. Carbohydrate metabolism genes and pathways in insects: insights from the honey bee genome 査読有り

    T. Kunieda, T. Fujiyuki, R. Kucharski, S. Foret, S. A. Ament, A. L. Toth, K. Ohashi, H. Takeuchi, A. Kamikouchi, E. Kage, M. Morioka, M. Beye, T. Kubo, G. E. Robinson, R. Maleszka

    INSECT MOLECULAR BIOLOGY 15 (5) 563-576 2006年10月

    出版者・発行元:BLACKWELL PUBLISHING

    DOI: 10.1111/j.1365-2583.2006.00677.x  

    ISSN:0962-1075

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    Carbohydrate-metabolizing enzymes may have particularly interesting roles in the honey bee, Apis mellifera, because this social insect has an extremely carbohydrate-rich diet, and nutrition plays important roles in caste determination and socially mediated behavioural plasticity. We annotated a total of 174 genes encoding carbohydrate-metabolizing enzymes and 28 genes encoding lipid-metabolizing enzymes, based on orthology to their counterparts in the fly, Drosophila melanogaster, and the mosquito, Anopheles gambiae. We found that the number of genes for carbohydrate metabolism appears to be more evolutionarily labile than for lipid metabolism. In particular, we identified striking changes in gene number or genomic organization for genes encoding glycolytic enzymes, cellulase, glucose oxidase and glucose dehydrogenases, glucose-methanol-choline (GMC) oxidoreductases, fucosyltransferases, and lysozymes.

  58. Insights into social insects from the genome of the honeybee Apis mellifera 査読有り

    George M. Weinstock, Gene E. Robinson, Richard A. Gibbs, Kim C. Worley, Jay D. Evans, Ryszard Maleszka, Hugh M. Robertson, Daniel B. Weaver, Martin Beye, Peer Bork, Christine G. Elsik, Klaus Hartfelder, Greg J. Hunt, Evgeny M. Zdobnov, Gro V. Amdam, Marcia M. G. Bitondi, Anita M. Collins, Alexandre S. Cristino, H. Michael G. Lattorff, Carlos H. Lobo, Robin F. A. Moritz, Francis M. F. Nunes, Robert E. Page, Zila L. P. Simoes, Diana Wheeler, Piero Carninci, Shiro Fukuda, Yoshihide Hayashizaki, Chikatoshi Kai, Jun Kawai, Naoko Sakazume, Daisuke Sasaki, Michihira Tagami, Stefan Albert, Geert Baggerman, Kyle T. Beggs, Guy Bloch, Giuseppe Cazzamali, Mira Cohen, Mark David Drapeau, Dorothea Eisenhardt, Christine Emore, Michael A. Ewing, Susan E. Fahrbach, Sylvain Foret, Cornelis J. P. Grimmelikhuijzen, Frank Hauser, Amanda B. Hummon, Jurgen Huybrechts, Andrew K. Jones, Tatsuhiko Kadowaki, Noam Kaplan, Robert Kucharski, Gerard Leboulle, Michal Linial, J. Troy Littleton, Alison R. Mercer, Timothy A. Richmond, Sandra L. Rodriguez-Zas, Elad B. Rubin, David B. Sattelle, David Schlipalius, Liliane Schoofs, Yair Shemesh, Jonathan V. Sweedler, Rodrigo Velarde, Peter Verleyen, Evy Vierstraete, Michael R. Williamson, Seth A. Ament, Susan J. Brown, Miguel Corona, Peter K. Dearden, W. Augustine Dunn, Michelle M. Elekonich, Tomoko Fujiyuki, Irene Gattermeier, Tanja Gempe, Martin Hasselmann, Tatsuhiko Kadowaki, Eriko Kage, Azusa Kamikouchi, Takeo Kubo, Robert Kucharski, Takekazu Kunieda, Marce D. Lorenzen, Natalia V. Milshina, Mizue Morioka, Kazuaki Ohashi, Ross Overbeek, Christian A. Ross, Morten Schioett, Teresa Shippy, Hideaki Takeuchi, Amy L. Toth, Judith H. Willis, Megan J. Wilson, Karl H. J. Gordon, Ivica Letunic, Kevin Hackett, Jane Peterson, Adam Felsenfeld, Mark Guyer, Michel Solignac, Richa Agarwala, Jean Marie Cornuet, Monique Monnerot, Florence Mougel, Justin T. Reese, Dominique Vautrin, Joseph J. Gillespie, Jamie J. Cannone, Robin R. Gutell, J. Spencer Johnston, Michael B. Eisen, Venky N. Iyer, Vivek Iyer, Peter Kosarev, Aaron J. Mackey, Victor Solovyev, Alexandre Souvorov, Katherine A. Aronstein, Katarina Bilikova, Yan Ping Chen, Andrew G. Clark, Laura I. Decanini, William M. Gelbart, Charles Hetru, Dan Hultmark, Jean-Luc Imler, Haobo Jiang, Michael Kanost, Kiyoshi Kimura, Brian P. Lazzaro, Dawn L. Lopez, Jozef Simuth, Graham J. Thompson, Zhen Zou, Pieter De Jong, Erica Sodergren, Miklos Csuroes, Aleksandar Milosavljevic, Kazutoyo Osoegawa, Stephen Richards, Chung-Li Shu, Laurent Duret, Eran Elhaik, Dan Graur, Juan M. Anzola, Kathryn S. Campbell, Kevin L. Childs, Derek Collinge, Madeline A. Crosby, C. Michael Dickens, L. Sian Grametes, Christina M. Grozinger, Peter L. Jones, Mireia Jorda, Xu Ling, Beverly B. Matthews, Jonathan Miller, Craig Mizzen, Miguel A. Peinado, Jeffrey G. Reid, Susan M. Russo, Andrew J. Schroeder, Susan E. St Pierre, Ying Wang, Pinglei Zhou, Huaiyang Jiang, Paul Kitts, Barbara Ruef, Anand Venkatraman, Lan Zhang, Gildardo Aquino-Perez, Charles W. Whitfield, Susanta K. Behura, Stewart H. Berlocher, Walter S. Sheppard, Deborah R. Smith, Andrew V. Suarez, Neil D. Tsutsui, Xuehong Wei, David Wheeler, Paul Havlak, Bingshan Li, Yue Liu, Erica Sodergren, Angela Jolivet, Sandra Lee, Lynne V. Nazareth, Ling-Ling Pu, Rachel Thorn, Viktor Stolc, Thomas Newman, Manoj Samanta, Waraporn A. Tongprasit, Charles Claudianos, May R. Berenbaum, Sunita Biswas, Dirk C. de Graaf, Rene Feyereisen, Reed M. Johnson, John G. Oakeshott, Hilary Ranson, Mary A. Schuler, Donna Muzny, Joseph Chacko, Clay Davis, Huyen Dinh, Rachel Gill, Judith Hernandez, Sandra Hines, Jennifer Hume, LaRonda Jackson, Christie Kovar, Lora Lewis, George Miner, Margaret Morgan, Ngoc Nguyen, Geoffrey Okwuonu, Heidi Paul, Jireh Santibanez, Glenford Savery, Amanda Svatek, Donna Villasana, Rita Wright

    NATURE 443 (7114) 931-949 2006年10月

    出版者・発行元:NATURE PUBLISHING GROUP

    DOI: 10.1038/nature05260  

    ISSN:0028-0836

    eISSN:1476-4687

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    Here we report the genome sequence of the honeybee Apis mellifera, a key model for social behaviour and essential to global ecology through pollination. Compared with other sequenced insect genomes, the A. mellifera genome has high A+T and CpG contents, lacks major transposon families, evolves more slowly, and is more similar to vertebrates for circadian rhythm, RNA interference and DNA methylation genes, among others. Furthermore, A. mellifera has fewer genes for innate immunity, detoxification enzymes, cuticle-forming proteins and gustatory receptors, more genes for odorant receptors, and novel genes for nectar and pollen utilization, consistent with its ecology and social organization. Compared to Drosophila, genes in early developmental pathways differ in Apis, whereas similarities exist for functions that differ markedly, such as sex determination, brain function and behaviour. Population genetics suggests a novel African origin for the species A. mellifera and insights into whether Africanized bees spread throughout the New World via hybridization or displacement.

  59. Associative visual learning, color discrimination, and chromatic adaptation in the harnessed honeybee Apis mellifera L. 査読有り

    Sayaka Hori, Hideaki Takeuchi, Kentaro Arikawa, Michiyo Kinoshita, Naoko Ichikawa, Masami Sasaki, Takeo Kubo

    JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY 192 (7) 691-700 2006年7月

    出版者・発行元:SPRINGER

    DOI: 10.1007/s00359-005-0091-4  

    ISSN:0340-7594

    eISSN:1432-1351

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    We studied associative visual learning in harnessed honeybees trained with monochromatic lights associated with a reward of sucrose solution delivered to the antennae and proboscis, to elicit the proboscis extension reflex (PER). We demonstrated five properties of visual learning under these conditions. First, antennae deprivation significantly increased visual acquisition, suggesting that sensory input from the antennae interferes with visual learning. Second, covering the compound eyes with silver paste significantly decreased visual acquisition, while covering the ocelli did not. Third, there was no significant difference in the visual acquisition between nurse bees, guard bees, and foragers. Fourth, bees conditioned with a 540-nm light stimulus exhibited light-induced PER with a 618-nm, but not with a 439-nm light stimulus. Finally, bees conditioned with a 540-nm light stimulus exhibited PER immediately after the 439-nm light was turned off, suggesting that the bees reacted to an afterimage induced by prior adaptation to the 439-nm light that might be similar to the 540-nm light.

  60. Division of labor in the honey bee (Apis mellifera): the role of tyramine beta-hydroxylase 査読有り

    Herman K. Lehman, David J. Schulz, Andrew B. Barron, Lydia Wraight, Chris Hardison, Sandra Whitney, Hideaki Takeuchi, Rajib K. Paul, Gene E. Robinson

    JOURNAL OF EXPERIMENTAL BIOLOGY 209 (14) 2774-2784 2006年7月

    出版者・発行元:COMPANY OF BIOLOGISTS LTD

    DOI: 10.1242/jeb.02296  

    ISSN:0022-0949

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    The biogenic amine octopamine (OA) is involved in the regulation of honey bee behavioral development; brain levels are higher in foragers than bees working in the hive, especially in the antennal lobes, and treatment causes precocious foraging. We measured brain mRNA and protein activity of tyramine beta-hydroxylase (T beta h), an enzyme vital for OA synthesis, in order to begin testing the hypothesis that this enzyme is responsible for the rising levels of OA during honey bee behavioral development. Brain OA levels were greater in forager bees than in bees engaged in brood care, as in previous studies, but T beta h activity was not correlated with bee behavior. T beta h mRNA levels, however, did closely track OA levels during behavioral development, and T beta h mRNA was localized to previously identified octopaminergic neurons in the bee brain. Our results show that the transcription of this neurotransmitter synthetic enzyme is associated with regulation of social behavior in honey bees, but other factors may be involved.

  61. Differential expression of HR38 in the mushroom bodies of the honeybee brain depends on the caste and division of labor 査読有り

    Y Yamazaki, K Shirai, RK Paul, T Fujiyuki, A Wakamoto, H Takeuchi, T Kubo

    FEBS LETTERS 580 (11) 2667-2670 2006年5月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/j.febslet.2006.04.016  

    ISSN:0014-5793

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    We used a cDNA microarray to identify genes expressed in a caste (worker)- and division of labor (nurse bees or foragers)-dependent manner in the honeybee brain. Among the identified genes, one encoded a putative orphan receptor (HR38) homologue that mediates ecdysteroid-signaling. Real-time reverse transcription-polymerase chain reaction indicated that expression of this gene is higher in forager brains, as compared to nurse bees and queens. In the forager brain, expression was concentrated in a subset of the mushroom body neurons, suggesting that ecdysteroid-signaling in the mushroom bodies might be involved in the division of labor of the workers. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  62. Functional analysis of MbLK-1, a transcription factor preferentially expressed in the mushroom body of the honeybee brain 査読有り

    Korefumi Nakamura, Takekazu Kunieda, Hideaki Takeuchi, Akiko Wakamoto, Takeo Kubo

    ZOOLOGICAL SCIENCE 22 (12) 1482-1482 2005年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  63. Cytochrome P450CYP307A1/spook: A regulator for ecdysone synthesis in insects 査読有り

    T Namiki, R Niwa, T Sakudoh, K Shirai, H Takeuchi, H Kataoka

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 337 (1) 367-374 2005年11月

    出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE

    DOI: 10.1016/j.bbrc.2005.09.043  

    ISSN:0006-291X

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    The prothoracic gland (PG) has essential roles in synthesizing and secreting a steroid hormone called ecdysone that is critical for molting and metamorphosis of insects. However, little is known about the genes controlling ecdysteroidogenesis in the PG. To identify genes functioning in the PG of the silkworm, Bombyx mori, we used differential display PCR and focused on a cytochrome P450 gene designated Cyp307a1. Its expression level positively correlates with a change in the hemolymph ecdysteroid titer. In addition, Drosophila Cyp307a1 is encoded in the spook locus, one of the Halloween mutant family members showing a low ecdysone titer in vivo, suggesting that Cyp307a1 is involved in ecdysone synthesis. While Drosophila Cyp307a1 is expressed in the early embryos and adult ovaries, the expression is not observed in the PGs of embryos or third instar larvae. These results suggest a difference in the ecdysone synthesis pathways during larval development in these insects. (c) 2005 Elsevier Inc. All rights reserved.

  64. MBR-1, a novel helix-turn-helix transcription factor, is required for pruning excessive neurites in Caenorhabditis elegans 査読有り

    E Kage, Y Hayashi, H Takeuchi, T Hirotsu, H Kunitomo, T Inoue, H Arai, Y Iino, T Kubo

    CURRENT BIOLOGY 15 (17) 1554-1559 2005年9月

    出版者・発行元:CELL PRESS

    DOI: 10.1016/j.cub.2005.07.057  

    ISSN:0960-9822

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    In the developing brain, excessive neurites are actively pruned in the construction and remodeling of neural circuits. We demonstrate for the first time that the pruning of neurites occurs in the simple neural circuit of Caenorhabditis elegans and that a novel transcription factor, MBR-1, is involved in this process. We identified MBR-1 as a C. elegans ortholog of Mblk-1, a transcription factor that is expressed preferentially in the mushroom bodies of the honeybee brain [1]. Although Mblk-1 homologs are conserved among animal species, their roles in the nervous system have never been analyzed. We used C. elegans as an ideal model animal for analysis of neuronal development. mbr-1 is expressed in various neurons in the head and tail ganglia. A comparison of the morphology of mbr-1-expressing neurons revealed that excessive neurites connecting the left and right AIM interneurons are eliminated during larval stages in wild-type but are sustained through the adult stage in the mbr-1 mutant. In addition, mbr-1 expression is regulated by UNC-86, a POU domain transcription factor, and the pruning of the excessive AIM connection is impaired in the unc-86 mutant. These findings provide an important clue for further genetic dissection of neurite pruning.

  65. Identification and characterization of P450 Cyp307a1 from the silkworm, Bombyx mori and the fruit fly, Drosophila melanogaster. 査読有り

    T. Namiki, R. Niwa, K. Shirai, H. Takeuchi, H. Kataoka

    Biochem. Biophys. Res. Commun. 337, 367-374 2005年

    DOI: 10.1016/j.bbrc.2005.09.043  

  66. Gene expression of ecdysteroid-regulated gene E74 of the honeybee in ovary and brain 査読有り

    RK Paul, H Takeuchi, Y Matsuo, T Kubo

    INSECT MOLECULAR BIOLOGY 14 (1) 9-15 2005年1月

    出版者・発行元:BLACKWELL PUBLISHING LTD

    DOI: 10.1111/j.1365-2583.2004.00524.x  

    ISSN:0962-1075

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    To facilitate studies of hormonal control in the honeybee (Apis mellifera L.), a cDNA for a honeybee homologue of the ecdysteroid-regulated gene E74 (AmE74) was isolated and its expression was analysed. Northern blot analysis indicated strong expression in the adult queen abdomen, and no significant expression in the adult drone and worker abdomens. In situ hybridization demonstrated that this gene was expressed selectively in the ovary and gut in the queen abdomen. Furthermore, this gene was also expressed selectively in subsets of mushroom body interneurones in the brain of the adult worker bees. These findings suggest that AmE74 is involved in neural function as well as in reproduction in adult honeybees.

  67. [Molecular ethology using the honeybee as a model animal]. 査読有り

    Takeuchi H, Kunieda T, Tokuhiro-Sawata M, Park JM, Fujiyuki T, Kubo T

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 49 (16) 2542-2548 2004年12月

    出版者・発行元:16

    ISSN:0039-9450

  68. Identification and analysis of the minimal promoter activity of a novel noncoding nuclear RNA gene, AncR-1, from the honeybee (Apis mellifera L.) 査読有り

    M Sawata, H Takeuchi, T Kubo

    RNA-A PUBLICATION OF THE RNA SOCIETY 10 (7) 1047-1058 2004年7月

    出版者・発行元:COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT

    DOI: 10.1261/rna.5231504  

    ISSN:1355-8382

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    Previously, we identified a gene for a noncoding nuclear RNA, termed Ks-1, that is expressed preferentially in a restricted set of neurons in the honeybee brain. In the present study, we identified another novel gene, termed AncR-1, whose transcripts were localized to nuclei in the whole cortex region of the honeybee brain, as a candidate novel noncoding nuclear RNA gene. RNA fluorescent in situ hybridization revealed that AncR-1 and Ks-1 transcripts were located in a distinct portion of a single neural nucleus, suggesting that they have distinct functions in brain neurons. cDNA cloning revealed that the AncR-1 transcripts were up to 7 kb in size, had mRNA-like structures, and were alternatively spliced. The reporter assay using Drosophila SL-2 cells demonstrated that a TATA box-like sequence located -30 bp upstream of the 5' end of AncR-1 cDNA had promoter activity. None of the alternatively spliced AncR-1 cDNA variants contained significant open reading frames, strongly suggesting that AncR-1 transcripts function as novel noncoding nuclear RNAs. Furthermore, in situ hybridization revealed that AncR-1 was expressed not only in the brain but also in the sex organs in the queen and drones and in the hypopharyngeal glands and oenocytes of the worker bees, suggesting that AncR-1 is involved in diverse organ functions. Some of the AncR-1 transcripts enriched in the nuclei of the hypopharyngeal glands were polyadenylated, indicating the presence of mRNA-like AncR-1 transcripts in the nuclei.

  69. Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera 査読有り

    H Takeuchi, A Yasuda, Y Yasuda-Kamatani, M Sawata, Y Matsuo, A Kato, A Tsujimoto, T Nakajima, T Kubo

    CELL AND TISSUE RESEARCH 316 (2) 281-293 2004年5月

    出版者・発行元:SPRINGER-VERLAG

    DOI: 10.1007/s00441-004-0865-y  

    ISSN:0302-766X

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    We have recently identified a tachykinin-related peptide (AmTRP) from the mushroom bodies (MBs) of the brain of the honeybee Apis mellifera L. by using direct matrix-assisted laser desorption/ionization with time-of-flight mass spectometry and have isolated its cDNA. Here, we have examined prepro-AmTRP gene expression in the honeybee brain by using in situ hybridization. The prepro-AmTRP gene is expressed predominantly in the MBs and in some neurons located in the optic and antennal lobes. cDNA microarray studies have revealed that AmTRP expression is enriched in the MBs compared with other brain regions. There is no difference in AmTRP-expressing cells among worker, queen, and drone brains, suggesting that the cell types that express the prepro-AmTRP gene do not change according to division of labor, sex, or caste. The unique expression pattern of the prepro-AmTRP gene suggests that AmTRPs function as neuromodulators in the MBs of the honeybee brain.

  70. Novel insect picorna-like virus identified in the brains of aggressive worker honeybees 査読有り

    T Fujiyuki, H Takeuchi, M Ono, S Ohka, T Sasaki, A Nomoto, T Kubo

    JOURNAL OF VIROLOGY 78 (3) 1093-1100 2004年2月

    出版者・発行元:AMER SOC MICROBIOLOGY

    DOI: 10.1128/JVI.78.3.1093-1100.2004  

    ISSN:0022-538X

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    To identify candidate genes involved in the aggressive behavior of worker honeybees, we used the differential display method to search for RNAs exclusively detected in the brains of aggressive workers that had attacked a hornet. We identified a novel, 10,152-nucleotide RNA, termed Kakugo RNA. Kakugo RNA encodes a protein of 2,893 amino acid residues that shares structural features and sequence similarities with various picorna-like virus polyproteins, especially those from sacbrood virus, which infects honeybees. The Kakugo protein contains several domains that correspond to the virion protein, helicase, protease, and RNA-dependent RNA polymerase domains of various picorna-like virus polyproteins. When the worker bee tissue lysate was subjected to sucrose density gradient centrifugation, Kakugo RNA, except for the material at the bottom, was separated into two major peaks. One of the peaks corresponded to the position of Kakugo mRNA, and the other corresponded to the position of the poliovirus virion. These results suggest that the Kakugo RNA exists as an mRNA-like free RNA and virion RNA in the honeybee. Furthermore, injection of the lysate supernatant from the attacker heads into the heads of noninfected bees resulted in a marked increase in Kakugo RNA. These results demonstrate that Kakugo RNA is a plus-strand RNA of a novel picorna-like virus and that the brains of aggressive workers are infected by this novel virus. Kakugo RNA was detected in aggressive workers but not in nurse bees or foragers. In aggressive workers, Kakugo RNA was detected in the brain but not in the thorax or abdomen, indicating a close relation between viral infection in the brain and aggressive worker behaviors.

  71. Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads 査読有り

    H Takeuchi, A Yasuda, Y Yasuda-Kamatani, T Kubo, T Nakajima

    INSECT MOLECULAR BIOLOGY 12 (3) 291-298 2003年6月

    出版者・発行元:BLACKWELL PUBLISHING LTD

    DOI: 10.1046/j.1365-2583.2003.00414.x  

    ISSN:0962-1075

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    Using a combination of MALDI-TOF and on-line capillary HPLC/Q-Tof mass spectroscopy, we identified and determined the amino acid sequence of a novel neuropeptide in the brain of the honeybee Apis mellifera L., termed AmTRP peptide (Apis mellifera tachykinin-related peptide), related to insect tachykinin. A cDNA for a prepro-protein (prepro-AmTRP) of AmTRP was isolated and determined to encode seven AmTRPs 1-7. Northern blot analysis indicated that the prepro-AmTRP gene is expressed differentially in the nurse bee, forager, queen and drone heads. Strong expression was detected in the queen and forager heads, while weak and almost no significant expression was detected in the nurse and drone heads, respectively. These results suggest that AmTRP peptide functions as a neuromodulator and/or hormone, associated with sex-specific or age/division of labour-selective behaviour and/or physiology of the honeybees.

  72. Identification and characterization of Mlr1,2: two mouse homologues of Mblk-1, a transcription factor from the honeybee brain 査読有り

    T Kunieda, JM Park, H Takeuchi, T Kubo

    FEBS LETTERS 535 (1-3) 61-65 2003年1月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/S0014-5793(02)03858-9  

    ISSN:0014-5793

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    We previously identified the Mblk-1 gene in the honeybee brain, which encodes a transcription factor containing two DNA binding motifs, termed RHF1 and 2 (Takeuchi et al. (2001) Insect Mol. Biol. 121, 134-140). Here, we identified two mouse Mblk1 homologues, Mlr1 and Mlr2. Both encode proteins containing a single DNA-binding motif highly conserved with RHF2 and activate transcription mediated by a DNA element recognized by honeybee Mblk-1. Mlr1 was expressed predominantly in the spermatocytes of the testis, while Mlr2 was expressed in various tissues other than testis. Mlr1 transcripts were lost in the testis of W/W-nu mutant mice, suggesting a role in spermatogenesis. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

  73. Identification and punctate nuclear localization of a novel noncoding RNA, Ks-1, from the honeybee brain 査読有り

    M Sawata, D Yoshino, H Takeuchi, A Kamikouchi, K Ohashi, T Kubo

    RNA-A PUBLICATION OF THE RNA SOCIETY 8 (6) 772-785 2002年6月

    出版者・発行元:CAMBRIDGE UNIV PRESS

    DOI: 10.1017/S1355838202028790  

    ISSN:1355-8382

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    We identified a novel gene, Ks-1, which is expressed preferentially in the small-type Kenyon cells of the honeybee brain. This gene is also expressed in some of the large soma neurons in the brain and In the suboesophageal ganglion. Reverse transcription-polymerase chain reaction experiments Indicated that Ks-1 transcripts are enriched in the honeybee brain. cDNA cloning revealed that the consensus Ks-1 cDNA is over 17 kbp and contains no significant open reading frames. Furthermore, fluorescent in situ hybridization revealed that Ks-1 transcripts are located in the nuclei of the neural cells, accumulating In some scattered spots. These findings demonstrate that Ks-1 encodes a novel class of noncoding nuclear RNA and is possibly involved in the regulation of neural functions.

  74. Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray 査読有り

    H Takeuchi, T Fujiyuki, K Shirai, Y Matsuo, A Kamikouchi, Y Fujinawa, A Kato, A Tsujimoto, T Kubo

    FEBS LETTERS 513 (2-3) 230-234 2002年2月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/S0014-5793(02)02319-0  

    ISSN:0014-5793

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    To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP3) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP3-mediated Ca2+ signaling is enhanced in these neurons. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

  75. DNA-binding properties of Mblk-1, a putative transcription factor from the honeybee 査読有り

    JM Park, T Kunieda, H Takeuchi, T Kubo

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 291 (1) 23-28 2002年2月

    出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE

    DOI: 10.1006/bbrc.2002.6397  

    ISSN:0006-291X

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    We previously identified a gene, Mblk-1, that encodes a putative transcription factor with two DNA-binding motifs expressed preferentially in the honeybee brain [H. Takeuchi et al., Insect Mol. Biol. 10, 487-494 (2001)]. In the present study, we identified its preferred binding sequence as 5-CCCTATCGATCG-ATCTCTACCT-3' and characterized its DNA-binding properties using truncated Mblk-1 mutants. An electrophoretic mobility shift assay revealed that the full-length Mblk-1 binds to the sequence with high affinity, whereas each truncated DNA-binding motif of Mblk-1 binds with much lower affinities. An in vitro pull-down assay indicated that each DNA-binding motif affords homodimeric bindings, suggesting that Mblk-1 functions as a dimer. (C) 2002 Elsevier Science (USA).

  76. Identification of a novel gene, Mblk-1, that encodes a putative transcription factor expressed preferentially in the large-type Kenyon cells of the honeybee brain 査読有り

    H Takeuchi, E Kage, M Sawata, A Kamikouchi, K Ohashi, M Ohara, T Fujiyuki, T Kunieda, K Sekimizu, S Natori, T Kubo

    INSECT MOLECULAR BIOLOGY 10 (5) 487-494 2001年10月

    出版者・発行元:BLACKWELL SCIENCE LTD

    DOI: 10.1046/j.0962-1075.2001.00288.x  

    ISSN:0962-1075

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    Mushroom bodies (MBs) are considered to be involved in higher-order sensory processing in the insect brain. To identify the genes involved in the intrinsic function of the honeybee MBs, we searched for genes preferentially expressed therein, using the differential display method. Here we report a novel gene encoding a putative transcription factor (Mblk-1) expressed preferentially in one of two types of intrinsic MB neurones, the large-type Kenyon cells, which makes Mblk-1 a candidate gene involved in the advanced behaviours of honeybees. A putative DNA binding motif of Mblk-1 had significant sequence homology with those encoded by genes from various animal species, suggesting that the functions of these proteins in neural cells are conserved among the animal kingdom.

  77. Concentrated expression of Ca2+/calmodulin-dependent protein kinase II and protein kinase C in the mushroom bodies of the brain of the honeybee Apis mellifera L 査読有り

    A Kamikouchi, H Takeuchi, M Sawata, S Natori, T Kubo

    JOURNAL OF COMPARATIVE NEUROLOGY 417 (4) 501-510 2000年2月

    出版者・発行元:WILEY-LISS

    DOI: 10.1002/(SICI)1096-9861(20000221)417:4<501::AID-CNE8>3.0.CO;2-4  

    ISSN:0021-9967

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    We have previously used the differential display method to identify a gene that is expressed preferentially in the mushroom bodies of worker honeybees and to show that it encodes a putative inositol 1,4,5-trisphosphate receptor(IP3R) homologue (Kamikouchi et al. [1998] Biochem. Biophys. Res. Commun. 242:181-186). In the present study, we examined whether the expression of some of the genes for proteins involved in the intracellular Ca2+ signal transduction is also concentrated in the mushroom bodies of the honeybee by isolating cDNA fragments that encode the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and protein kinase C (PKC) homologues of the honeybee, In situ hybridization analysis revealed that the expression of these genes was also concentrated in the mushroom bodies of the honeybee brain: The CaMKII gene was expressed preferentially in the large-type Kenyon cells of the mushroom bodies, whereas that for PKC was expressed in both the large and small types of Kenyon cells. The expression of the genes for IP3R and CaMKII was concentrated in the mushroom bodies of the queen and drone as well as in those of the worker bee. Furthermore, the enzymatic activities of CaMKII and PKC were found to be higher in the mushroom bodies/central bodies than in the optic and antennal lobes of the worker bee brain. These results suggest that the function of the intracellular Ca2+ signal transduction is enhanced in Kenyon cells in comparison to other neuronal cell types in the honeybee brain. (C) 2000 Wiley-Liss, Inc.

  78. Molecular biology concerning to the honeybee sociality: how can we approach the evolution of insect sociality?

    Kubo, T., Ohashi, K., Sawata, M., Kamikouchi, A., Takeuchi, H.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 45 (7) 2000年

    ISSN:0039-9450

  79. Soldier caste-specific gene expression in the mandibular glands of Hodotermopsis japonica (Isoptera : Termopsidae) 査読有り

    T Miura, A Kamikouchi, M Sawata, H Takeuchi, S Natori, T Kubo, T Matsumoto

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 96 (24) 13874-13879 1999年11月

    出版者・発行元:NATL ACAD SCIENCES

    DOI: 10.1073/pnas.96.24.13874  

    ISSN:0027-8424

    詳細を見る 詳細を閉じる

    Although "polymorphic castes" in social insects are well known as one of the most important phenomena of polyphenism, few studies of caste-specific gene expressions have been performed in social insects. To identify genes specifically expressed in the soldier caste of the Japanese damp-wood termite Hodotermopsis japonica, we employed the differential-display method using oligo(dT) and arbitrary primers, compared mRNA from the heads of mature soldiers and pseudergates (worker caste), and identified a clone (PCR product) 329 bp in length termed SOLI. Northern blot analysis showed that the SOLI mRNA is about 1.0 kb in length and is expressed specifically in mature soldiers, but not in pseudergates, even in the presoldier induction by juvenile hormone analogue, suggesting that the product is specific for terminally differentiated soldiers. By using the method of 5'- and 3'-rapid amplification of cDNA ends, we isolated the full length of SOLI cDNA, which contained an ORF with a putative signal peptide at the N terminus. The sequence showed no significant homology with any other known protein sequences. In situ hybridization analysis showed that SOLI is expressed specifically in the mandibular glands. These results strongly suggest that the SOLI gene encodes a secretory protein specifically synthesized in the mandibular glands of the soldiers. Histological observations revealed that the gland actually develops during the differentiation into the soldier caste.

  80. Preferential expression of the gene for a putative inositol 1,4,5-trisphosphate receptor homologue in the mushroom bodies of the brain of the worker honeybee Apis mellifera L. 査読有り

    A Kamikouchi, H Takeuchi, M Sawata, K Ohashi, S Natori, T Kubo

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 242 (1) 181-186 1998年1月

    出版者・発行元:ACADEMIC PRESS INC

    DOI: 10.1006/bbrc.1997.7870  

    ISSN:0006-291X

    詳細を見る 詳細を閉じる

    A gene expressed preferentially in the mushroom bodies of the brain of the worker honeybee Apis mellifera L. was identified by the differential display method and its cDNA was isolated. The cDNA fragment of 534 bp (clone Al) contained an open reading frame encoding 177 amino acid residues having 78, 72, 70, 59 and 55% sequence identities with the inositol 1,4,5-trisphosphate (IP3) receptors of Drosophila melanogaster, Xenopus Laevis and humans (types 1, 2 and 3), respectively, suggesting that it encodes a putative IP3 receptor homologue of the honeybee. In situ hybridization revealed that the gene encoding clone Al was expressed preferentially in the mushroom bodies and not in the optic lobes, antennal lobes and central bodies; in the mushroom body, it was expressed strongly in the large type Kenyon cells and weakly in the small type Kenyon cells. Reverse transcription polymerase chain reaction analysis showed that the gene was expressed strongly in the head and weakly in the antennae, legs, thorax, and abdomen. These results suggest that the Al gene product plays a crucial role in neural transmission in the mushroom bodies of the worker bee brain. (C) 1998 Academic Press.

  81. Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II 査読有り

    T Ito, QH Xu, H Takeuchi, T Kubo, S Natori

    FEBS LETTERS 385 (1-2) 21-24 1996年4月

    出版者・発行元:ELSEVIER SCIENCE BV

    DOI: 10.1016/0014-5793(96)00340-7  

    ISSN:0014-5793

    詳細を見る 詳細を閉じる

    Previously, we characterized a rat cDNA for testis-specific transcription elongation factor S-II (SII-T1) (Q. Xu et al., J. Biol. Chem. 269, 3100-3103 (1994)). Here, we isolated a 335-bp fragment of the cDNA for mouse SII-T1, and used it to examine the expression of the SII-T1 gene in the testis by in situ hybridization. The results indicated that the SII-T1 gene is expressed exclusively in spermatocytes, showing no appreciable expression in spermatogonia, spermatids, or Leydig cells. RT-PCR experiments using testis RNA from W\W-v mutant mice also suggested that SII-T1 is a specific transcription elongation factor essential for spermatogenesis.

  82. Molecular cloning of cDNA and analysis of expression of the gene for alpha-glucosidase from the hypopharyngeal gland of the honeybee Apis mellifera L 査読有り

    K Ohashi, M Sawata, H Takeuchi, S Natori, T Kubo

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 221 (2) 380-385 1996年4月

    出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS

    DOI: 10.1006/bbrc.1996.0604  

    ISSN:0006-291X

    詳細を見る 詳細を閉じる

    Previously, we identified and purified an alpha-glucosidase with a molecular mass of 70 kDa from a homogenate of the hypopharyngeal gland of the older worker bee (forager bet) Apis mellifera L. (T. Kubo et al., J. Biochem., 1996, 119, 291-295). Here, we isolated and sequenced a cDNA for the alpha-elucosidase. The cDNA encoded a protein consisting of 650 amino acids, which had high sequence identity with fruit fly and mosquito possible maltase gene products. RT-PCR showed that the gene was expressed specifically in the hypopharyngeal gland Of the forager bee. (C) 1996 Academic Press, Inc.

  83. Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L) with age and/or role 査読有り

    T Kubo, M Sasaki, J Nakamura, H Sasagawa, K Ohashi, H Takeuchi, S Natori

    JOURNAL OF BIOCHEMISTRY 119 (2) 291-295 1996年2月

    出版者・発行元:JAPAN BIOCHEMICAL SOC

    ISSN:0021-924X

    詳細を見る 詳細を閉じる

    The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis using affinity-purified antibodies against three major proteins (50, 56, and 64 kDa) of the nurse-bee gland showed that they are synthesized selectively and secreted as bee-milk proteins. Immunofluorescence study showed that the proteins condense in the duct after secretion from acini. However, a major 76-kDa protein synthesized specifically in the forager-bee hypopharyngeal gland was identified as an alpha-glucosidase. Therefore, the hypopharyngeal gland seems to have two distinct states differentiated by synthesizing of different major proteins depending on the age-dependent role change.

︎全件表示 ︎最初の5件までを表示

MISC 65

  1. 配偶者選択におけるオキシトシンの役割の性的二形性

    横井佐織, 成瀬清, 亀井保博, 安齋賢, 木下政人, 水戸麻理, 岩崎信太郎, 井ノ上俊太郎, 奥山輝大, 中川真一, YOUNG Larry J., 竹内秀明

    日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020年

  2. メダカの行動学 研究の最先端 招待有り

    王 牧芸, 横井佐織, 奥山 輝大, 竹内 秀明

    生物の科学 遺伝 72 2018年11月

  3. 社会的記憶が"異性の好み"を生み出す神経機構 (特集 記憶ふたたび)

    奥山 輝大, 竹内 秀明

    生体の科学 67 (1) 71-75 2016年1月

    出版者・発行元:金原一郎記念医学医療振興財団 ; 1949-

    DOI: 10.11477/mf.2425200403  

    ISSN:0370-9531

  4. ヒメダカの三角関係(雄、雄、雌)における勝者を決めるホルモン 招待有り

    横井佐織, 坂本竜哉, 坂本浩隆, 竹内秀明

    海洋と生物 37 (6) 591-597 2015年12月

    出版者・発行元:生物研究社

    ISSN:0285-4376

  5. 「社会脳」の進化的起源の解明を目指して~社会的意思決定を担う終神経GnRH3ニューロン~

    奥山輝大, 竹内秀明

    生化学 87 (5) 605-608 2015年10月25日

    DOI: 10.14952/SEIKAGAKU.2015.870605  

    ISSN:0037-1017

  6. 愛と分子(9)「異性の好み」を生み出す分子と神経 : 記憶・経験に基づく選択

    奥山 輝大, 竹内 秀明

    現代化学 (534) 28-31 2015年9月

    出版者・発行元:東京化学同人

    ISSN:0386-961X

  7. メダカを用いた分子遺伝学的手法による魚類「社会脳」の分子神経基盤の解明

    奥山輝大, 竹内秀明

    比較生理生化学 31 (3) 106-112 2014年9月30日

    出版者・発行元:日本比較生理生化学会

    DOI: 10.3330/hikakuseiriseika.31.106  

    ISSN:0916-3786

    詳細を見る 詳細を閉じる

    社会を形成する動物は,協調や世話,攻撃や拒絶など他メンバーに対する行動を柔軟に選択する。高度な社会性行動を営む動物は,他メンバーを記憶・識別し,相手との社会関係を理解し,社会的文脈に沿って意思決定する高次脳機能を持つ。これまで高度な社会行動に関わる脳機能の研究は主にヒトを対象にして進んできたが,分子・神経細胞レベルで解析するためには,適切なモデル生物が必要になる。著者らはこの問題にアプローチする目的で,分子遺伝学のモデル生物であるメダカに着目してきた。著者らはメダカの社会行動(群れ行動,社会学習,メスの配偶者選択,オスの配偶者防衛行動)の行動実験系を確立する過程で,メダカは個体認知に基づく高度な社会行動を示すことを発見した。メダカのメスは「見知らぬオス」を拒絶し,そばにいた「見知ったオス」を視覚認知して配偶相手として選択する傾向がある。さらに著者らは2014年に分子遺伝学及び電気生理学的手法を組み合わせて,終神経 GnRH3ニューロンがオスを配偶相手として受け入れるか否かのスイッチとして機能することを示した。一方で著者らは2013年にメダカ胚において赤外線レーザー誘起遺伝子発現操作を用いて,予定脳の微小領域に熱刺激依存に Cre/LoxP 組換えを誘導する技術を確立し,特定の脳領域において外来遺伝子の発現を誘導する遺伝子操作法を確立した。また最新の遺伝子改変技術(TALEN法,CRISPR/Casシステム)を用いることで,わずか5日の実験行程で目的遺伝子に変異を入れることが可能になった。今後,メダカは魚類「社会脳」の分子神経基盤を解析する優れたモデルになると期待される。

  8. セイヨウミツバチApis mellifera L.脳でキノコ体に高度に選択的に発現する3遺伝子の同定

    末次翔太, 竹内秀明, 國枝武和, 久保健雄

    日本動物学会大会予稿集 84th 158 2013年8月12日

  9. メダカの配偶者選択の分子神経基盤

    奥山輝大, 横井佐織, 阿部秀樹, 磯江泰子, 末廣勇司, 今田はるか, 島田敦子, 川崎隆史, 弓場俊輔, 谷口善仁, 亀井保博, 田中実, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明

    日本動物学会大会予稿集 84th 137 2013年8月12日

  10. Analysis of neural mechanism for mate-guarding behavior, using small fish medaka

    Saori Yokoi, Teruhiro Okuyama, Hideaki Takeuchi, Takeo Kubo

    NEUROSCIENCE RESEARCH 71 E265-E265 2011年

    出版者・発行元:ELSEVIER IRELAND LTD

    DOI: 10.1016/j.neures.2011.07.1156  

    ISSN:0168-0102

  11. Neural basis underlying sexual selection in medaka fish

    Hideaki Takeuchi, Saori Yokoi, Yuji Suehiro, Haruka Imada, Minoru Tanaka, Takashi Kawasaki, Atsuko Shimada, Hideki Abe, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Teruhiro Okuyama

    NEUROSCIENCE RESEARCH 71 E24-E24 2011年

    出版者・発行元:ELSEVIER IRELAND LTD

    DOI: 10.1016/j.neures.2011.07.100  

    ISSN:0168-0102

  12. p53 mutation caused suppression of neurogenesis in the juvenile medaka fish brain

    Yasuko Isoe, Teruhiro Okuyama, Yoshihito Taniguchi, Takeo Kubo, Hideaki Takeuchi

    NEUROSCIENCE RESEARCH 71 E239-E240 2011年

    出版者・発行元:ELSEVIER IRELAND LTD

    DOI: 10.1016/j.neures.2011.07.1048  

    ISSN:0168-0102

  13. 視覚依存の配偶者選別において行動異常を示すメダカ変異体の同定

    奥山輝大, 末廣勇司, 今田はるか, 阿部秀樹, 島田敦子, 田中実, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明

    生化学 ROMBUNNO.1P-0845 2010年

    ISSN:0037-1017

  14. Analysis of the brain substructures using region-preferential genes of the honeybee

    金子 九美, 森本 舞, 堀 沙耶香, 中岡 貴義, 竹内 秀明, 久保 健雄

    第32回日本分子生物学会要旨集 2009年12月

  15. Analysis of neural mechanism for the optomotor response of the medaka fish 査読有り

    Yuji Suehiro, Teruhiro Okuyama, Haruka Imada, Kiyoshi Naruse, Daisuke Kurabayashi, Atsuko Shimada, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    Proceeding of the Third International Symposium on Mobiligence (査読有り国際紀要) 308-311 2009年11月

  16. Analysis of Schooling-like Behavior of Medaka Fish and Identification of a Telencephalon-and-Hypothalamus-Preferential Gene in the Medaka Brain 査読有り

    Haruka Imada, Yuji Suehiro, Teruhiro Okuyama, Daisuke Kurabayashi, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    Proceeding of the Third International Symposium on Mobiligence (査読有り国際紀要) 295-298 2009年11月

  17. 2A1-E15 外部抑制と自己抑制による自律分散システムの空間的作業分化

    春山 弘貴, 倉林 大輔, 竹内 秀明

    ロボティクス・メカトロニクス講演会講演概要集 2009 "2A1-E15(1)"-"2A1-E15(4)" 2009年5月25日

    出版者・発行元:一般社団法人日本機械学会

    詳細を見る 詳細を閉じる

    Honeybee which is social insect changes the work with age in day and does division of labor in a colony. A feature of this division is one of the suitability of creatures, a model of controlling juvenile hormone of honeybee which increases with age in day was proposed as a conventional research. In this model, there are 2 repressing effects for juvenile hormone, one is generated in inside, the other is generated when contact with other honeybees. But contact is not defined because this model is not considered about spatial relation. So we expand the model to be considered about contact on a colony, and define 2 task modes with hormone content for express division of labor. Next, we derive local and whole rates of division of labor in a colony by analyzing equilibrium conditions of the hormone equations. And we verify that these rates have stability for disturbance.

  18. ミツバチの"ダンスコミュニケーション"の神経基盤の理解にむけて--野外と室内における新しい2つの実験系

    木矢剛智, 堀沙耶香, 竹内秀明

    蛋白質核酸酵素 53 (11) 1368-1374 2008年9月

    出版者・発行元:共立出版

    ISSN:0039-9450

  19. Differential expression of hormone receptor-like in 38 (HR38) in the honeybee brain depending on the caste differentiation and division of labor of the workers

    Y. Yamazaki, K. Shirai, R. K. Paul, T. Fujiyuki, A. Wakamoto, H. Takeuchi, T. Kubo

    FEBS JOURNAL 275 144-144 2008年6月

    出版者・発行元:WILEY-BLACKWELL

    ISSN:1742-464X

  20. Identification and analysis of genes that are expressed strongly in the optic lobes in the honeybee brain

    K. Kaneko, S. Hori, H. Takeuchi, R. K. Paul, T. Fujiyuki, K. Shirai, A. Wakamoto, T. Kubo

    FEBS JOURNAL 275 147-147 2008年6月

    出版者・発行元:BLACKWELL PUBLISHING

    ISSN:1742-464X

  21. G227 ミツバチ脳の視葉で主要に発現する2種類の遺伝子の解析

    金子九美, 堀沙耶香, 竹内秀明, PaulRajib Kumar, 藤幸知子, 白井健一, 若本明子, 久保健雄

    日本応用動物昆虫学会大会講演要旨 (52) 129-129 2008年3月12日

    出版者・発行元:日本応用動物昆虫学会

  22. 小型魚類の適応的行動(視運動反応, 群れ行動)の脳情報処理原理とその神経・分子的基盤の解明を目指して

    竹内 秀明, 末廣 勇司, 今田 はるか, 奥山 輝大, 久保 健雄

    計測と制御 = Journal of the Society of Instrument and Control Engineers 46 (12) 922-927 2008年1月

    出版者・発行元:The Society of Instrument and Control Engineers

    DOI: 10.11499/sicejl1962.46.922  

    ISSN:0453-4662

    詳細を見る 詳細を閉じる

    研究紹介に日本語解説

  23. 線虫C.elegansをモデルとした神経突起除去の分子機構の解析

    林悠, 広津崇亮, 中臺枝里子, 竹内秀明, 國友博文, 飯野雄一, 久保健雄

    生化学 4P-0722 2007年

    ISSN:0037-1017

  24. Analysis of adaptive behaviors of small fishes by using combination of mathematical modeling and molecular biological approach. 査読有り

    Suehiro Y, Imada H, Okuyama T, Kubo T, Takeuchi H†, †Corresponding

    Proceeding of the Second International Symposium on Mobiligence(査読有り国際紀要). 219-222 2007年

  25. Quantitative analysis of developmental processes of following behaviors in medaka using a newly established assay system

    Haruka Imada, Kenichi Shirai, Yuuji Suehiro, Kazuki Horikawa, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    ZOOLOGICAL SCIENCE 23 (12) 1187-1187 2006年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  26. Analysis of mathematical principle of information processing and the molecular/neural basis that underlie optomotor response of small fishes

    Yuuji Suehiro, Haruka Imada, Kazuki Horikawa, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

    ZOOLOGICAL SCIENCE 23 (12) 1187-1187 2006年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  27. Physiological transition of honeybee workers induced by loss of the queen

    Takayoshi Nakaoka, Hideaki Takeuchi, Kenichi Shirai, Rajib Paul, Tomoko Fujiyuki, Akiko Wakamoto, Takeo Kubo

    ZOOLOGICAL SCIENCE 23 (12) 1225-1226 2006年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  28. Identification of candidate genes involved in social behavior in the honeybee brain. Using honeybee genome data base

    Hideaki Takeuchi

    ZOOLOGICAL SCIENCE 23 (12) 1129-1129 2006年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  29. Identification and analysis of genes that are expressed strongly in the optic lobes in the honeybee brain

    Kumi Kaneko, Sayaka Hori, Hideaki Takeuchi, Rajib Paul, Tomoko Fujiyuki, Kenichi Shirai, Akiko Wakamoto, Takeo Kubo

    ZOOLOGICAL SCIENCE 23 (12) 1191-1191 2006年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  30. Analysis of the significance and mechanism of neurite elimination using C-elegans as a model

    Yu Hayashi, Takaaki Hirotsu, Eriko Kage, Hideaki Takeuchi, Hirofumi Kunitomo, Yuichi Iino, Takeo Kubo

    NEUROSCIENCE RESEARCH 55 S185-S185 2006年

    出版者・発行元:ELSEVIER IRELAND LTD

    ISSN:0168-0102

    eISSN:1872-8111

  31. Prevalence of Kakugo virus in the honeybee Apis mellifera L. population analyzed by quantitative reverse transcription-PCR

    Tomoko Fujiyuki, Hideaki Takeuchi, Seii Ohka, Masato Ono, Akio Nomoto, Takeo Kubo

    ZOOLOGICAL SCIENCE 22 (12) 1482-1482 2005年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  32. Identification and analysis of genes whose expression change in the brains of the workers in queenless colonies

    Takayoshi Nakaoka, Hideaki Takeuchi, Takeo Kubo

    ZOOLOGICAL SCIENCE 22 (12) 1514-1514 2005年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  33. Identification of brain protein(s) expressed differentially in the worker honeybees in relation to their division of labors using proteome analysis

    Yuko Uno, Tomoko Fujiyuki, Mizue Morioka, Hideaki Takeuchi, Takeo Kuhn

    ZOOLOGICAL SCIENCE 22 (12) 1482-1482 2005年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  34. Acetylcholinesterase is queen-selectively transcribed in honeybee brain and ovary

    Yuichi Takeuchi, Hideaki Takeuchi, Maya Ohara, Kenta Asahina, Takeo Kubo

    ZOOLOGICAL SCIENCE 22 (12) 1510-1510 2005年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  35. 線虫 C.elegans をモデルとした神経突起除去の分子機構の解析

    林悠, 鹿毛枝里子, 広津崇亮, 国友博文, 竹内秀明, 飯野雄一, 久保健雄

    日本分子生物学会年会講演要旨集 28th 294 2005年11月25日

  36. 線虫において神経突起のせん断及びきゅう覚行動可塑性に関わる新規転写因子MBR‐1によって発現制御される遺伝子群の同定

    三田芳久, 鹿毛枝里子, 林悠, 国友博文, 飯野雄一, 久保健雄, 竹内秀明

    日本分子生物学会年会講演要旨集 28th 294 2005年11月25日

  37. A318 光刺激-口吻伸展反射連合学習系を利用したセイヨウミツバチ(Apis mellifera L.)の波長識別能力と残像現象の解析(一般講演)

    堀沙耶香, 竹内秀明, 蟻川謙太郎, 木下充代, 佐々木正己, 市川直子, 久保健雄

    日本応用動物昆虫学会大会講演要旨 (49) 64-64 2005年3月1日

    出版者・発行元:日本応用動物昆虫学会

  38. Kakugo virus from brains of aggressive worker honeybees

    T Fujiyuki, H Takeuchi, M Ono, S Ohka, T Sasaki, A Nomoto, T Kubo

    ADVANCES IN VIRUS RESEARCH, VOL 65 65 1-+ 2005年

    出版者・発行元:ELSEVIER ACADEMIC PRESS INC

    DOI: 10.1016/S0065-3527(05)65001-4  

    ISSN:0065-3527

  39. Analysis of molecular basis of associative visual learning in the honeybee Apis mellifera L.

    Sayaka Hori, Hideaki Takeuchi, Kentaro Arikawa, Michiyo Kinoshita, Masami Sasaki, Naoko Ichikawa, Takeo Kubo

    ZOOLOGICAL SCIENCE 21 (12) 1315-1316 2004年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  40. Identification and characterization of a novel gene, QM1, expressed preferentially in the brain of the queen honeybee (Apis mellifera L.)

    Kenichi Shirai, Maya Ohara, Hideaki Takeuchi, Takeo Kubo

    ZOOLOGICAL SCIENCE 21 (12) 1343-1343 2004年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  41. A novel C-elegans transcription factor MBR-1 is involved in olfactory plasticity and axon pruning

    Yu Hayashi, Eriko Kage, Takaaki Hirotsu, Hirofumi Kunitomo, Hideaki Takeuchi, Yuichi Iino, Takeo Kubo

    ZOOLOGICAL SCIENCE 21 (12) 1318-1318 2004年12月

    出版者・発行元:ZOOLOGICAL SOC JAPAN

    ISSN:0289-0003

  42. ミツバチをモデル生物とした分子行動生物学

    竹内秀明, 國枝武和, 徳廣, 澤, 美由紀

    蛋白質核酸酵素 49 (16) 2542-2548 2004年12月

    出版者・発行元:共立出版

    ISSN:0039-9450

  43. 線虫C.elegansの嗅覚可塑性に関わる転写因子MBR-1の軸索除去における機能解析(Analysis of the function of MBR-1,a C.elegans transcription factor involved in olfactory plasticity,in axon pruning)

    林 悠, 鹿毛 枝里子, 広津 崇亮, 國友 博文, 竹内 秀明, 飯野 雄一, 久保 健雄

    神経化学 43 (2-3) 526-526 2004年8月

    出版者・発行元:日本神経化学会

    ISSN:0037-3796

  44. セイヨウミツバチにおける光刺激-口吻伸展反射連合学習.

    堀沙耶香, 竹内秀明, 市川直子, 佐々木正己, 久保健雄

    日本応用動物昆虫学会大会講演要旨. 114 2004年

  45. セイヨウミツバチの攻撃的な働き蜂の脳から同定されたKakugoウイルス

    藤幸知子, 竹内秀明, 久保健雄

    ミツバチ科学 25 (4) 145-151 2004年

    出版者・発行元:玉川大学ミツバチ科学研究施設

    ISSN:0388-2217

  46. 線虫 C.elegans のきゅう覚可塑性に関わる転写因子MBR‐1の軸索形成における機能解析

    林悠, 鹿毛枝里子, 広津崇亮, 国友博文, 竹内秀明, 飯野雄一, 久保健雄

    日本分子生物学会年会プログラム・講演要旨集 26th 898 2003年11月25日

  47. IDENTIFICATION OF HONEYBEE BRAIN REGION-SELECTIVE GENES BY A COMBINATION OF DIFFERENTIAL DISPLAY METHOD AND cDNA MICROARRAY(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Kumar Paul Rajib, Takeuchi Hideaki, Fujiyuki Tomoko, Matsuo Yuko, Kato Azusa, Tsujimoto Atsumi, Kubo Takeo

    Zoological science 20 (12) 1581-1581 2003年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  48. PREPRO-TACHYKININ GENE EXPRESSION IN THE HONEYBEE APIS MELLIFERA L. BRAIN(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Takeuchi Hideaki, Yasuda Akikazu, Yasuda Yoshimi, Matsuo Yuko, Kato Azusa, Tsujimoto Atsumi, Nakajima Terumi, Kubo Takeo

    Zoological science 20 (12) 1588-1588 2003年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  49. ASSOCIATIVE VISUAL LEARNING IN HONEYBEES APIS MELLIFERA L.(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Hori Sayaka, Takeuchi Hideaki, Sasaki Masami, Ichikawa Naoko, Kubo Takeo

    Zoological science 20 (12) 1587-1587 2003年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  50. IDENTIFICATION AND CHARACTERIZATION OF A NOVEL GENE, TERMED QM1, EXPRESSED PREFERENTIALLY IN THE BRAIN OF THE QUEEN HONEYBEE (APIS MELLIFERA L.)(Physiology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Shirai Kenichi, Ohara Maya, Takeuchi Hideaki, Kubo Takeo

    Zoological science 20 (12) 1587-1587 2003年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  51. セイヨウミツバチにおける光刺激-PER連合学習

    堀沙耶香, 竹内秀明, 佐々木正己, 市川直子, 久保健雄

    日本応用動物昆虫学会大会講演要旨. 93 2003年

  52. A HONEYBEE (APIS MELLIFERA L.) BRAIN MAP DISSECTED AT THE MOLECULAR LEVEL USING DIFFERENTIAL DISPLAY AND DNA MICROARRAY METHOD(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Takeuchi Hideaki, Rajib Kumar Paul, Fujiyuki Tomoko, Matsuo Yuko, Shirai Kennichi, Kato Azusa, Tsujimoto Atsumi, Kubo Takeo

    Zoological science 19 (12) 1472-1472 2002年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  53. IDENTIFICATION AND CHARACTERIZATION OF A NOVEL NUCLEAR RNA FACTOR, KS-1, EXPRESSED IN THE HONEYBEE BRAIN(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Sawata Miyuki, Yoshino Daisuke, Takeuchi Hideaki, Kamikouchi Azusa, Ohashi Kazuaki, Kubo Takeo

    Zoological science 19 (12) 1472-1472 2002年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  54. IDENTIFICATION OF A NOVEL PICORNA-LIKE VIRUS GENOME RNA IN THE BRAIN OF THE AGGRESSIVE HONEYBEES(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Fujiyuki Tomoko, Takeuchi Hideaki, Ono Masato, Ohka Seii, Nomoto Akio, Sasaki Tetsuhiko, Kubo Takeo

    Zoological science 19 (12) 1472-1472 2002年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  55. Identification of two mouse homologs of Mblk-1, that encodes a mushroom body-specific transcription factor of the honeybee.(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Kunieda T., Takeuchi H., Kage E., Park J., Kubo T.

    Zoological science 18 106-106 2001年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  56. Characterization of the C. elegans homolog of Mblk-1 encoding a putative transcription factor that is expressed in the honey bee brain(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Kage Eriko, Takeuchi Hideaki, Inoue Takao, Suzuki Yuka, Sugimoto Asako, Yamamoto Masayuki, Kubo Takeo

    Zoological science 18 106-106 2001年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  57. Identification and characterization of genes induced in brains of the honeybees that have attacked the hornets.(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Fujiyuki T., Ono M., Takeuchi H., Kubo T.

    Zoological science 18 106-106 2001年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  58. Identification of a novel gene, termed QM1, expressed preferentially in the brain of the queen honeybee(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

    Shirai K., Ohara M., Takeuchi H., Kubo T.

    Zoological science 18 106-106 2001年

    出版者・発行元:Zoological Society of Japan

    ISSN:0289-0003

  59. Identification of the gene for a putative transcription factor (Mblk-1) expressed preferentially in the large-type Kenyon cells of the honey bee brain

    Takeuchi H, Kage E, Kamikouchi A, Kubo T

    Zoological science 18 91-91 2001年

    出版者・発行元:社団法人日本動物学会

    ISSN:0289-0003

  60. ミツバチの高次行動を支える脳の分子機構の解析

    竹内秀明, 上川内あづさ, 鹿毛枝理子, 久保健雄

    ミツバチ科学 22 (3) 113-120 2000年12月

    出版者・発行元:玉川大学

    ISSN:0388-2217

  61. ミツバチの社会性をめぐる分子生物学——昆虫の社会性の進化にどのようにアプローチするか

    久保健雄, 大橋一晶, 澤田美由紀, 上川内あづさ, 竹内秀明

    蛋白質核酸酵素 45 (7) 1229-1236 2000年5月

    出版者・発行元:7

    ISSN:0039-9450

  62. ミツバチのキノコ体に選択的に発現する遺伝子の同定と解析

    竹内秀明, 上川内あづさ, 澤田美由紀

    比較生理生化学 16 (4) 266-277 1999年12月

    出版者・発行元:日本比較生理生化学会

    DOI: 10.3330/hikakuseiriseika.16.266  

    ISSN:0916-3786 1881-9346

  63. ミツバチの分子社会生物学

    久保 健雄, 大橋 一晶, 竹内 秀明, 澤田 美由紀, 上川内 あづさ, 名取 俊二

    化学と生物 34 (12) 793-798 1997年1月25日

    出版者・発行元:学会出版センタ-

    DOI: 10.1271/kagakutoseibutsu1962.34.793  

    ISSN:0453-073X

  64. セイヨウミツバチ(Apis mellifera)脳のキノコ体のLarge type Kenyon細胞に特異的に発現する遺伝子の同定と解析

    竹内 秀明, 大橋 一晶, 澤田 美由紀, 名取 俊二, 久保 健雄

    日本分子生物学会年会プログラム・講演要旨集 19 528-528 1996年8月1日

  65. ミツバチ働き蜂の行動変化に伴う下咽頭腺由来ロイヤルゼリー蛋白およびα-グルコシダーゼの遺伝子発現解析

    大橋 一晶, 竹内 秀明, 澤田 美由紀, 名取 俊二, 久保 健雄

    日本分子生物学会年会プログラム・講演要旨集 19 827-827 1996年8月1日

︎全件表示 ︎最初の5件までを表示

書籍等出版物 9

  1. Perspectives on the Function of Oxytocin in Fish and Mammals (Chapter7)

    Yokoi S, Young LJ, Takeuchi H

    "Spectrum of Sex - The Molecular Bases that Induce Various Sexual Phenotypes" edited by M Tanaka and M Tachibana, Springer Nature 2022年

  2. 動物行動の分子生物学 (新・生命科学シリーズ)

    久保 健雄, 上川内 あづさ, 竹内 秀明, 奥山 輝大, 太田 次郎, 浅島 誠, 長田 敏行, 赤坂 甲治

    裳華房 2014年7月30日

    ISBN: 4785358580

  3. 身近な動物を使った実験〈4〉ミツバチ・コオロギ・スズメガ

    三共出版 2009年10月

    ISBN: 4782705832

  4. 身近な動物を使った実験〈4〉ミツバチ・コオロギ・スズメガ

    三共出版 2009年10月

    ISBN: 4782705832

  5. 分子昆虫学 ―ポストゲノムの昆虫研究―

    共立出版 2009年8月25日

    ISBN: 4320056957

  6. 分子昆虫学 ―ポストゲノムの昆虫研究―

    共立出版 2009年8月25日

    ISBN: 4320056957

  7. 昆虫ミメティックス―昆虫の設計に学ぶ (Advanced Biomimetics Series)

    下澤 楯夫, 針山 孝彦

    エヌティーエス 2008年10月10日

    ISBN: 4860431979

  8. 生物薬科学実験講座「タンパク質」4.cDNAクローニング」

    廣川書店 2008年4月

    ISBN: 9784567170109

  9. “Gene expression in the Honeybee Brain Mushroom Body and its Gene Orthologues” Evolution of Nervous Systems

    Hideaki Takeuchi, Edited by Kaas H

    Elsevier 2006年12月

    ISBN: 9780123925602

    詳細を見る 詳細を閉じる

    http://japan.elsevier.com/products/books/ENS.pdf

︎全件表示 ︎最初の5件までを表示

講演・口頭発表等 46

  1. メダカの三角関係を生み出すホルモンと脳の仕組み 招待有り

    竹内秀明

    世界脳週間オンラインサイエンスカフェ 2022年3月13日

  2. Future Aspects of Social Neuroscience Using Medaka Fish

    The Social Brain 2.0: Social Neuroscience In The Post Pandemic Era 2021年9月30日

  3. メダカの個体認知を介した配偶者選択に関わる脳の分子神経基盤 招待有り

    竹内秀明

    日本化学会 2021年3月19日

  4. 性的パートナー選択にバイアスを与える脳の内部状態の神経基盤 招待有り

    竹内秀明

    日本認知科学会第36回大会 2019年9月5日

  5. メダカの恋の分子神経基盤 -恋ごころの進化的ルーツを探る- 招待有り

    竹内秀明

    日本科学者会議岡山支部例会よもやま話の会 2016年2月29日

  6. Exploring the neural geography of the social brain using medaka fish 国際会議 招待有り

    Hideaki Takeuchi, Keynote Lecture

    17th Australia & New Zealand Zebrafish Meeting 2016年2月5日

  7. Exploring the neural geography of the social brain using medaka fish 国際会議 招待有り

    竹内秀明, Keynote Lecture

    3rd Medaka Strategic Meeting, Flinders, Australia 2016年2月1日

  8. Neural mechanism of female preference mediated by social memory 国際会議 招待有り

    竹内秀明

    Symposium on Memory and Mind, Tohoku Forum for Creativity 2015年9月29日

  9. メダカの恋の三角関係を生み出す分子神経機構 招待有り

    竹内秀明

    第23回日本行動神経内分泌研究会サテライトミーティング 2015年9月19日

  10. メダカの三角関係を生み出す分子基盤 招待有り

    竹内秀明

    日本動物学会関東支部公開講演会「動物の行動を科学する」 2015年8月1日

  11. Proximate and ultimate causes of socially-regulated mating preference in medaka fish 国際会議 招待有り

    竹内秀明

    2nd Strategic Meeting for Medaka Research 2014年4月10日

  12. Socially-regulated mating preference in medaka fish and its neural mechanism 国際会議 招待有り

    竹内秀明

    日本比較生理生化学会2013年大会 2013年7月13日

  13. Integrative analysis of sexual behaviors in medaka fish: From neurons to behavioral evolution 招待有り

    Hideaki Takeuchi

    "Evolution of the Brain” supported by CPIS, SOKENDAI" 2012年11月13日

  14. メダカから探る恋心の神経機構 招待有り

    竹内秀明

    第22回東京大学理学部公開講演会 「三者三様 理学が挑む」 2012年11月4日

  15. Neural Basis Underlying Sexual Selection in Medaka Fish 国際会議 招待有り

    竹内秀明

    脳回路網の形成と機能発現の統合的研究の展開(GCOEシンポジウム) 2012年1月

  16. Neural Basis Underlying Sexual Selection in Medaka Fish 国際会議 招待有り

    竹内秀明

    The 1st Strategic Meeting for Medaka Research 2011年11月23日

  17. Neural basis underlying sexual selection in medaka fish 国際会議 招待有り

    Hideaki Takeuchi

    The 34th Annual Meeting of the Japan Neuroscience Society "Vertebrate brain functions elucidated in zebrafish and medaka" 2011年9月15日

  18. Neural basis underlying female mating preference in medaka fish 国際会議 招待有り

    Hideaki Takeuchi

    the 8th International Congress on Comparative Physiology and Biochemistry 2011年6月2日

  19. 配偶行動の分子神経基盤:実験動物の研究からヒトの 恋愛感情はどこまで理解できるのか?

    竹内秀明

    基礎生物学研究所重点共同利用シンポジウム 2011年3月

  20. 「サービス・イノベーションへの期待」 国際会議

    竹内秀明, パネリスト

    「理学は実業の問題を解決できるか」 2011年2月

  21. Analysis of Social behaviors in Adult Medaka Fish 国際会議 招待有り

    竹内秀明

    The BRH Seminar "Evolution & Diversity of Fishes" 2010年6月

  22. メダカの社会性行動を生み出す脳機能の解析 招待有り

    竹内秀明

    日本分子生物学会春季シンポジウム「身体・脳システムの心理表出行動と分子動態基盤」 2010年6月

  23. Molecular/ neural basis involved in social interaction among medaka fish

    竹内秀明

    第87回日本生理学会大会「比較的単純なモデル動物を用いた運動・行動研究」 2010年5月

  24. Analysis of Social Interactions Using Adult Medaka Fish 招待有り

    Hideaki Takeuchi

    Common Themes and New Concepts in Sensory Formation, The 18th CDB Meeting. 2009年4月

  25. メダカの個体間相互作用を定量する行動実験系の確立

    竹内秀明

    北大行動知能講演会 2009年3月

    詳細を見る 詳細を閉じる

    メダカは日本国内で江戸時代からペットとして飼育され、「メダカの学校」と呼ばれる群れ行動や攻撃行動、また雄の求愛円舞(配偶行動)など多様な個体間相互作用の様式が観察されている。一方でメダカは発生や生殖生物学のモデル生物として遺伝子導入法やノックアウトの作出など洗練された分子遺伝学的手法が確立している。私達は将来的にメダカの行動様式に関わる脳機能を分子遺伝学的手法で解析する目的で、メダカの個体間相互作用を定量化する行動実験系の確立を行っている。これまでにメダカの配偶行動における雄と雌の性的動機を定量化する行動アッセイ系や二匹のメダカの同調的行動を定量化する行動アッセイ系を確立した。本講演ではこれらの個体間相互作用に影響を与える要因やその生物学的意義について議論していきたいと考えている。

  26. セイヨウミツバチ脳におけるエクダイソンに関わる遺伝子群の発現様式 招待有り

    竹内秀明

    第53回日本応用動物昆虫学会大会 2009年3月

    詳細を見る 詳細を閉じる

    第1回昆虫ホルモン談話会~幼若ホルモンとエクジソンの生理機能研究の新展開~

  27. Analysis of information processing and neural/ molecular basis underlying adaptive behaviors (optomotor response and coordinated behavior) of small fish

    Hideaki Takeuchi

    第18回インテリジェント・システム・シンポジウム 2008年10月

  28. Analysis of Social Interactions Using Adult Medaka Fish 国際会議 招待有り

    Hideaki Takeuchi

    The 54th NIBB Conference, New Frontiers for the Medaka Model, Genome, Bioresources and Biology 2008年

  29. ミツバチとショウジョウバエの比較分子神経生物学

    竹内秀明

    生研センターシンポジウム 2007年7月

  30. ミツバチの社会性行動とゲノムデーターベース

    Hideaki Takeuchi

    ニューロエソロジー談話会 2006年9月

  31. Molecular basis underlyning the mushroom body function of the honeybee brain 招待有り

    Hideaki Takeuchi

    Bee Vision and Learning, SECOND EUROPEAN CONFERENCE OF APIDOLOGY , Praha, Czech Republic 2006年9月

  32. Candidate genes involved in honeybee social behaviors 招待有り

    Hideaki Takeuchi

    Special Talk at Centre de Recherches sur la Cognition Animale CNRS - Université Paul Sabatier - Toulouse III, France 2006年9月

  33. Candidate genes involved in honeybee social behaviors 招待有り

    Hideaki Takeuchi

    Special Talk at Lehrstuhl für Neurobiologie Universität Konstanz, Germany 2006年3月

  34. Gene expression in honeybee mushroom bodies and its gene orthologues in non-social insects/invertebrates 国際会議 招待有り

    Hideaki Takeuchi

    3rd European Congress on Social Insects, Meeting of European Sections of the IUSSI, St. Petersburg, Russia 2005年8月

  35. Honeybee as a new model system for molecular neurobiology 国際会議 招待有り

    Hideaki Takeuchi

    第77回日本遺伝学会 公開講演会:神経遺伝学の現状と展望 2005年

  36. Future aspect of molecular neurobiology using a honeybee as a model system 国際会議 招待有り

    Hideaki Takeuchi

    The Gordon Research Conference, Neuroethology: Behavior, Evolution & Neurobiology (Oxford, United Kingdom) 2005年

  37. Honeybee as a new model system for molecular neurobiology 国際会議

    Hideaki Takeuchi

    Special Talk at Institute of Zoology, Department of Animal Physiology, University of Cologne, Germany 2005年

  38. Future aspect of molecular neurobiology using a honeybee mushroom body as a model system

    Hideaki Takeuchi

    Neuro 2004 2004年

    詳細を見る 詳細を閉じる

    日本神経化学会大会、日本神経科学大会、合同大会

  39. Honeybee as a new model system for molecular neurobiology 招待有り

    Hideaki Takeuchi

    International Symposium on Chemical Senses and Insect Behavior 2004年

  40. Honeybee as a new model system for molecular neurobiology 招待有り

    Hideaki Takeuchi

    Special Talk at Arizona Research Laboratories Division of Neurobiology, Arizona University 2004年

  41. Honeybee as a new model system for molecular neurobiology

    Hideaki Takeuchi

    Special Talk at San Diego University of California 2004年

  42. ミツバチが拓く脳分子生物学の未来

    竹内秀明

    基生研研究会「新しいモデル生物が拓く生物科学フロンティア」 2004年

  43. Molecular basis underlying honeybee social behavior.

    Hideaki Takeuchi

    International Union of Biological Sciences - Towards An Integrative Biology Symposium on "Biology of Learning" 2003年

  44. Molecular dissection of the mushroom body in the honeybee brain

    Hideaki Takeuchi

    Special Talk at Department of Entomology, University of Illinois 2003年

  45. ミツバチ脳において高次中枢(キノコ体)で機能する遺伝子群の同定

    竹内秀明

    Zoological Conference, 東京大学大学院理学系講義 2003年

  46. 脳行動状態系が社会行動選択にバイアスを与える分子神経機構の解明-メダカを動物モデルとして- 招待有り

    竹内秀明

    第50回日本神経精神薬理学会年会・第42回日本生物学的精神医学会年会・第4回日本精神薬学会総会(NPBPPP2020合同年会) 2020年8月22日

︎全件表示 ︎最初の5件までを表示

共同研究・競争的資金等の研究課題 23

  1. 魚類ソーシャルビジョンの分子・遺伝基盤の解明

    竹内 秀明

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Scientific Research (A)

    研究機関:Tohoku University

    2021年4月 ~ 2025年3月

  2. 社会脳ネットワークの全容解明 〜シングルセルトランスクリプトーム解析による高解 像度機能地図の作成〜

    2020年4月 ~ 2023年3月

  3. 配偶戦略の性スペクトラムを生み出す分子神経基盤の解明

    竹内 秀明

    2020年4月1日 ~ 2022年3月31日

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    メダカにおいてはオキシトシンシステムは配偶戦略の性差が発現するために必須のシステムであり(Yokoi et al 2020 PNAS)、オキシトシンシステムの標的ニューロンの性差を解明すれば、配偶戦略の性差を生み出す分子神経基盤を解明できる可能性がある。本研究ではオキシトシンシステムの標的ニューロン(oxtr1 発現ニューロン)に性差があることを検証することを目的として、 oxtr1 発現ニューロンが存在する終脳/視さく前野と視蓋において scRNA-seq(シングルセルトランスクリプトーム)を行なった。研究開始当初、魚類ではゼブラフィッシュ稚魚でscRNA-seqが実施されているが (Nat Biotechnol 2018) 、成魚脳でscRNA-seqを実施された例がなかった。そこで、本研究ではまずメダカ成体脳を用いて、scRNA-seqの実験系構築を行った。メダカ終脳と視蓋を用いて細胞分離条件の検討を行い、最終的に、メダカ終脳メスから 4,786 細胞を回収し、 1 細胞あたり、 79,738 リード/cell、1,040 遺伝子/cell, 2,198UMI/cell のデータを取得できた。過去のゼブラフィッシュ幼魚の全脳 を用いた報告と比較して、メダカ成魚脳でscRNA-seqの実験系の確立に成功したと判断した。また終脳はクラスタリング解析から 14 種類に分類できることがわかり、oxtr1 発現ニューロンを含む細胞腫を同定した。次に、終脳/視さく前野と視蓋を対象にオスとメスのシングルセルトランスクリプトームを実施した。それぞれの組織から分離した細胞を 10X Genomics Chromium により、分取、バーコーディング、ライブラリー作製することに成功したが、現在、次世代シークエンサーを用いてシーケンスを実施中である。

  4. 魚類「社会脳」の進化的起源と神経 動作原理の解明

    2020年4月 ~ 2022年3月

  5. メダカの顔認知の分子神経基盤の解明

    竹内 秀明, 王 牧芸, 横井 佐織

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    2018年4月1日 ~ 2021年3月31日

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    これまでにメダカは個体認知に基づく高度な社会行動を示すことを発見した。例えば、メスメダカは性行動前に長時間見ていたオスを視覚記憶し、個体認知を介して「親密性の高いオス」を配偶相手として選択し、「見知らぬオス」を拒絶する傾向がある(Science 2014)。さらにメスが「顔」を認知して、オスを見分けており、ヒトの心理学実験で有名な倒立顔効果がメダカにも生じることを見出した(elife 2017)。本年度はオキシトシン(OXT)とオキシトシン受容体(OXTR1, OXTR2)をコードする遺伝子の変異体を作成して行動解析を行った結果、oxtまたはoxtr1のどちらかの遺伝子を欠損すると、メスは異性の好みを失い、「見知らぬオス」を「親密性の高いオス」と同じように受け入れることを発見した。よってオキシトシンもメダカメスの個体認知を介した配偶者選択に必要であることを新たに見出した。一方で、メダカはオスとメスで配偶戦略が異なっており、メスには異性の好みがあるが、オスは特に異性の好みを持たない。しかし、変異体オスは同じ水槽で育った「親密なメス」に対する異常な好みを持つことを見出した。よってメダカにおいてオキシトシンはメスとオスで逆の効果を持つことを発見した。これに加え、オキシトシンの新規標的分子をしてC1qを同定した本年度の研究成果は2020年2月18日にProceedings of the National Academy of Sciences誌に掲載された(竹内が最終著者・責任著者)。東北大、北大、基礎生物学研究から共同プレスリリースを行った結果、NHKニュース(北海道版、全国版)等のメディア紹介された。

  6. 脳発達における外套の機能構築原理とその創発特性の検証

    竹内 秀明, 中村 遼平, 磯江 泰子, 亀井 保博, 野中 茂紀

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:Okayama University

    2016年7月19日 ~ 2019年3月31日

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    脊椎動物の大脳は,背側パリウム(大脳皮質に対応)と腹側のサブパリウム(大脳基底核に対応)に大別される。メダカではパリウムの各区画構造は孵化後の成長過程に生じる「新生ニューロンの細胞系譜単位」が「レゴブロック」のように組合わさることにより構築されることを見出した。さらにパリウム及び比較対象としてサブパリウムの細胞系譜単位を合計30個程度摘出して、ATAC-Seqによりオープンクロマチン領域を全ゲノムレベルで比較した。その結果、パリウムとサブパリウムのエピゲノムパターンは大きく異なっており、パリウムの各細胞系譜単位の間にも特徴的なエピゲノムパターンを存在することを発見した。

  7. 細胞系譜単位を介した終脳多様性・保存性の進化機構の解析

    磯江 泰子, 竹内 秀明, 中村 遼平

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up

    研究種目:Grant-in-Aid for Research Activity Start-up

    研究機関:Okayama University

    2016年8月26日 ~ 2018年3月31日

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    脊椎動物の「終脳(哺乳類では「大脳」に対応)」は、背側の「外套(大脳皮質)」と腹側の「外套下部(大脳基底核)」から成る。感覚統合・記憶学習に重要な外套は種間で多様化している。外套の種多様性が生じる機構は脳進化を考える上で重要だが不明である。発生期では種間で保存されているので、生後脳発達に着目する必要があると考え、顕著な脳発達が見られ、かつ種間で外套構造の多様化が見られる硬骨魚類のメダカを研究対象とした。成体終脳の新生神経細胞の体系的な構造解析や、解剖領域を構成する新生神経細胞の遺伝子発現制御解析を行った。その結果、外套の構築原理(区画構造の創出機構)を発見し、種間多様性が生じる機構を考察した。

  8. 個体記憶が異性の好みを生み出す神経動作原理の解明

    竹内 秀明

    2016年4月1日 ~ 2018年3月31日

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    集団で生活するいくつかの動物は個体認知能力を介して社会適応する。例えば,鳥類では種の90%以上は一夫一婦制を営み,お互いにパートナーを認知している。またいくつかの魚類も個体認知能力を持つ。順位制を持つ熱帯魚の一種(シクリッド)は集団メンバーの順位を記憶して,上位個体からは逃避し,下位個体には接近する。仲間を見分ける上で,ヒトやサルにおいては視覚系が最も重要であり,顔認知に特化した脳領域(顔領域)が大脳皮質に存在する。平成28年度はメダカの社会行動を解析した結果,メダカは個体認知を介して社会に適応していることを発見した。メダカのオスはメスを巡って競争し、メスは長時間そばにいた優位なオスを記憶・識別して性的パートナーとして受け入れる傾向がある。平成29年度はメダカの個体認知の視覚的な手がかりを探索した。体の一部を隠したオスと「お見合い」させることで,メスがオスを識別する際には,「顔」の視覚情報が特に重要であることを見出した。次にプリズムを使って上下逆さまになったオスとお見合いしても識別できないことを示した。さらにメスの識別能力を電気ショック学習実験により検定した。水槽を3つに区切り,中央にメス1匹,両端にオスを1匹ずつ入れ,メスに2匹の異なるオスを視覚的に提示した。 この状態で一方のオスに近づいた時に電気ショックを与える訓練を繰り返すと,メスはそのオスを見分けて避けるようになった。次にプリズムを使ってオスを上下逆さまに提示する と,メスはオスの識別能力は低下した。興味深いことに,メダカオスの代わりに2つの異なる物体を提示した場合,倒立しても物体識別能力は低下しなかった。このことからメダカの顔認知機構は物体認知機構と異なっていることが示唆された。よってメダカ脳においてもヒト同様に顔認知に特化した脳領域(顔領域)が存在する可能性がある。本研究成果は elife に掲載された。

  9. 小型魚類の社会脳の脳機能地図の作製 競争的資金

    竹内 秀明, 久保 健雄, 亀井 保博, 磯江 泰子, 横井 佐織

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    2014年4月1日 ~ 2017年3月31日

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    メダカの三者関係(オス、オス、メス)において、「オスがライバルオスよりもメスから近い位置を維持するように、ライバルオスとメスとの間に割り込む」という形で配偶者防衛を示すことを発見した。メダカ変異体を用いた行動実験の結果、バソトシンシステムが配偶者防衛においてライバルオスに勝つために必要であることを示した(PLoS Genetics 2015). さらに配偶者防衛行動は、ライバルオスの性行動を妨害するだけでなく「ライバルオスを記憶できないようにすることで、自らが配偶相手として選ばれる確率を上昇させる」という意義も存在することが示唆できた(Frontiers in Zoology, 2016).

  10. 社会認知を介した意思決定に関わる神経ネットワークの解明 競争的資金

    竹内 秀明

    2014年4月1日 ~ 2016年3月31日

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    <最初期遺伝子を検索・同定>本年度はメダカの個体記憶を介した配偶者選択に関わる神経ネットーワークを包括的に同定する目的で、メダカ脳で最初期遺伝子を検索・同定した。方法はPTZ(GABA阻害剤)添加によって誘導される癲癇依存に発現が誘導される遺伝子群をマイクロアレイによって検索した。約15,000遺伝子について発現量を比較したところ、18種類の遺伝子が有為に発現上昇することを定量的RT-PCRで確認した。そのうち2つの遺伝子を用いて脳活動のマッピングを行ったところ、メダカメスがオスと長時間見ていた時に活性化している脳領域(終脳背側の一部)を同定した。 <脳領域特異的な条件的遺伝子操作法の確立>これまでに、メダカの条件的遺伝子操作法を確立し、成体脳において細胞系譜単位(単一の神経幹細胞から発生したニューロン群)を遺伝学的にラベルすることに成功している。さらに一部の細胞系譜単位は終脳背側ではブロック化していることを見出している(PLoS One 8:e66597,2013)。本年度は終脳背側の一つの細胞系譜単位が(1)で同定した活性化脳領域に対応することを見出した。 <個体記憶を介した行動を定量化する新規行動実験系の確立>本年度は新規な行動実験系を2つ確立し、以下の2点を示した。(1) メダカの三角関係(オス、オス、メス)において、オスはライバルオスに配偶者防衛行動を示すことで、メスがライバルオスを記憶することを妨害している(Frontier in Zoology in press) 。メダカメスはそばにいたオスを記憶して、配偶者として選択する傾向があるので、メスがライバルオスを選択することを妨害していると考えられる。(2) メスは排卵前には視覚記憶したオスに選択的に近づき行動を示すが、排卵後はオスに対する選択性がなくなる(Zoological Science in press)。

  11. 花粉媒介行動の脳の分子神経基盤とその生態系への影響の解明

    竹内 秀明, 久保 健雄, WANG MU-YUN, WANG Mu-Yun

    2013年4月26日 ~ 2016年3月31日

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    外国人特別研究員のWang博士はマルハナバチのコロニーの中には「注意深い(careful) 個体」と「衝動的な(impulsive) 個体」が存在することを、採餌戦略の違いから明らかにした。注意深い個体は時間をかけて蜜源を選択するが、花蜜量が多く外敵が少ない蜜源を正確に選択する。一方で衝動的な個体は短時間で蜜源を選択するが、質の高い蜜源を正確に選択できないこと(Speed-accuracy tradeoffs)を見出した(Wang et al 2015)。さらに採餌戦略における個体差が脊椎動物にもある否かを確認する目的で、Wang博士はメダカを用いて行動実験系を確立した。メダカは遺伝的背景が同一である近交系が多数、確立・維持されている。そのため近交系ごとに採餌戦略が異なることを示せれば、採餌戦略の違いは遺伝的背景の違いによると考察することができる。先行研究では、メダカに餌を与えた時に視覚刺激を同時に与えると、視覚刺激と餌の情報を連合して学習し、訓練後は視覚刺激依存に近づき行動を示すことがわかっていた(PLoS ONE 8(9): e71685, 2013)。Wang博士は、別の視覚刺激に対して電気ショックを与える実験装置を付加し、報酬と罰の両方の情報が存在する環境で、採餌行動を定量化する行動実験を確立した。Wang博士は5種類の近交系メダカの採餌行動を比較した結果、系統ごとに採餌戦略が異なる傾向があったことから、遺伝学的背景が採餌戦略を規定している可能性が考えられる。

  12. メダカを用いた個体認知に関わる脳領域の検索 競争的資金

    竹内 秀明, 亀井 保博

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    研究種目:Grant-in-Aid for Challenging Exploratory Research

    研究機関:The University of Tokyo

    2013年4月1日 ~ 2015年3月31日

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    メダカのメスは「見知らぬオス」を拒絶し,そばにいた「見知ったオス」を視覚認知して性的パートナーとして選択する傾向がある。本研究では、GnRH3ニューロンがメダカの性的パートナー選択において中心的な役割を果たすことを見出した。(Science, 2014)。さらにメダカ終脳において部分的にCre/loxP組換えを誘導する遺伝子操作法(PLoS One 2013)及び、個体認知を検定する新規な行動実験系を確立した(PLoS One 2013)

  13. ミツバチ働き蜂の行動と生理状態を連動して制御する内分泌・神経機構の解析

    久保 健雄, 竹内 秀明, 國枝 武和

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:The University of Tokyo

    2011年4月1日 ~ 2014年3月31日

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    本研究課題では、ミツバチの社会性行動の基盤となる脳神経機構と生理機構の解明を目指した。その結果、脳高次中枢(キノコ体)を形成する大型ケニヨン細胞の細胞特性を明らかにすると共に、新規な「中間型」ケニヨン細胞を発見した。また、採餌蜂では小型ケニヨン細胞と一部の「中間型」ケニヨン細胞が興奮していること、さらにオオスズメバチに対する「熱殺蜂球」を形成しているニホンミツバチの脳では、クラスIIケニヨン細胞が興奮していることを示した。一方、若い働き蜂(育児蜂)が分泌するローヤルゼリーが、3種類の外分泌腺由来のタンパク質の「カクテル」であり、他個体の生理状態に影響しうる細胞増殖因子を含むことを示した。

  14. メダカの社会性行動を生み出す脳の分子神経基盤の解明 競争的資金

    竹内 秀明, 亀井 保博

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:The University of Tokyo

    2011年4月1日 ~ 2014年3月31日

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    メダカのメスがそばにいた異性を目で見て記憶し、性的パートナーとして積極的に受け入れることを発見した。オスとメスを透明なガラスで仕切ってお見合いさせておくと、メスは目で見ていた「そばにいたオス」の求愛をすぐに受け入れ、他の恋敵のオスはメスをめぐる闘いに敗北する。さらに、性的パートナーを受け入れる際に、拒絶から受け入れへとモードを切り替えるための神経細胞を同定した。お見合いをすると、メスの脳では終神経GnRH3ニューロンの電気的活動が活性化し、この神経細胞がメスの「恋ごころスイッチ」として機能することが明らかになった。

  15. 脊椎度物の社会性を生み出す脳神経基盤と行動法則の解明を目指した生医工連携研究の確立 競争的資金

    竹内秀明

    2011年4月 ~ 2013年3月

  16. 行動特性の個体差を規定する遺伝的要因の同定と社会形成における生態学的意義の解明 競争的資金

    竹内 秀明, 成瀬 清

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    研究種目:Grant-in-Aid for Challenging Exploratory Research

    研究機関:The University of Tokyo

    2011年 ~ 2012年

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    行動の「個性」には、生後の環境だけでなく遺伝的要因も影響すると考えられている。しかし、責任遺伝子の同定や、行動の多様性を産み出す遺伝子基盤の解析はあまり進んでいない。本研究では種内の遺伝的多様性の高いメダカ(Oryzias latipes)を用いて、行動形質に関わる遺伝子座を探索した。視覚刺激依存の逃避様反応に系統差が見られたため、2つの近交系、HNI-IIとHdrR-IIに視覚刺激を呈示し、反応を比較した。その結果、HNI-IIはHdrR-IIに比べて反応率が高く、馴れも生じにくかった。2つの近交系間でF2世代を作出し、量的形質遺伝子座(QTL)解析を行った結果、刺激への馴れにくさに関わる領域が16番染色体上に存在することが示唆された。近交系間の行動特性の違いは、行動の種内多様性を反映すると考えられる。

  17. ヒトの社会を支える脳神経基盤と行動法則の解明を目指した生医工連携研究の確立 競争的資金

    竹内秀明

    2010年4月 ~ 2011年3月

  18. メダカの個体間相互作用に関わる神経回路及び遺伝子の検索 競争的資金

    竹内 秀明

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    研究種目:Grant-in-Aid for Young Scientists (B)

    研究機関:The University of Tokyo

    2009年 ~ 2010年

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    メダカの個体間相互作用(群れ行動)を誘導する行動実験系を確立した。さらにメダカ脳において条件的遺伝子操作法を確立し、メダカの社会性行動に関わる候補遺伝子として神経ペプチドをコードする遺伝子群を同定した。

  19. 昆虫の社会行動を司る生理機能をモデルとした移動知研究 競争的資金

    三浦 徹, 竹内 秀明

    2008年 ~ 2009年

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    シロアリなどの社会性昆虫のコロニーには様々な機能を果たすカーストが存在し,複雑なコミュニケーションに基づく精巧な分業を行うことで社会行動を成立させている.また,メダカに見られるような群れ行動においても個体間の相互作用が重要な役割を果たす.我々は,動物にみられる組織的な行動とその分子基盤を明らかにすることで,生物社会の仕組みと進化を理解し,新たな生命システムを見いだすことを目的として,多角的に研究を進めている.本年度は,シロアリの分業を制御すると考えられるフェロモンの分析を精力的に行い,カースト特異的なタンパクや炭化水素の同定を行った.更に,社会行動の1つである防衛行動に焦点を当て,カーストによる行動の違いを見いだし,状況に応じて可塑的にカーストの行動が制御されていることを明らかとした.さらに,フェロモン分析が進展し,発生のゆらぎにより遺伝子ネットワークが構築されるというモデルの結果も得られた.更に魚の群れ行動についても行動学的および神経生物学的な知見が蓄積された.今後も引き続きそれらの知見や実験系を駆使し,社会行動の総合的な理解につなげたい.更に,包括的な社会システムの理解を目指し,新しい自律分散システムの設計など工学への応用に関しても貢献していきたい.

  20. 分子生物学の新しいモデルとしてのミツバチの開発と利用 (研究担当者) 競争的資金

    久保健雄

    2004年 ~ 2008年

  21. 昆虫の社会行動を統御するための脳分化の解析 競争的資金

    三浦 徹, 竹内 秀明, 尾崎 まみこ

    2006年 ~ 2007年

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    本年度は,昨年度同様,社会性昆虫であるオオシロアリを材料にして,ワーカーとソルジャーの分化に伴う脳機能の分化の解析を行った.行動観察により,カーストによる行動走性の相違を検証し,分子基盤や神経生理の解析を後に行う際の機能マッセイ系が構築された.また,この2つのカースト間において,脳の解剖学的相違,組織切片作成による詳細な細胞学的解析により食道下神経節に存在する大顎筋運動ニューロンが兵隊分化に伴い巨大化することを明らかにした.さらに,行動多型を維持する分子基盤の解明を具体的に推し進めるため,2者間の脳における遺伝子発現の相違をディファレンシャルディスプレイ法などにより検出した.その結果発現量に差のある有力な候補遺伝子がいくつか同定された.現在,遺伝子候補に関して,リアルタイムqPCRなどにより発現動態や発現部位の特定を試みるに至った.また,分担者の尾崎は,クロオオアリを用いて,巣仲間認識に関わる体表炭化水素組成の分析およびこの組成を認識する感覚毛における生理学的解析を進め,脳内の触角葉の糸球体において,感覚毛からの情報が集積されることが明らかとなった.また,分担者の竹内はミツバチのキノコ体特異的に発現する遺伝子の解析を精力的に進めた.その結果キノコ体ではエクダイソンシグナルに関わる遺伝子の発現が分業などにより特異的に変化することが突き止やられた.また行動アッセイ法を確立した.これらの知見を集積し,昆虫の高度な社会性を構築する脳内の分子基盤について詳しい考察がされ,成果を国際誌に投稿した.

  22. 小型魚類の群れ行動に関わる脳領域の同定と分子的基盤の解明 競争的資金

    竹内 秀明

    2006年 ~ 2007年

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    多くの魚は自然条件下では水流の中で周りの景色に定位するために、視野を一定に保とうと運動する性質がある(視運動反応)。視運動反応は研究室内で簡単に誘導することができる。方法は固定した円形水槽に魚を入れ、水槽外側で縦縞模様が描かれた円柱をモータで回転させると、水槽内の魚は周りの背景に定位するために縦縞を追いかけて回転運動をする。1972年にPartridgeらは大型水槽(直径10m)でタラなどの海水魚の魚群(20匹)を用いて視運動反応を誘導すると協調的に集団遊泳することを示している。本年度は小型水槽(直径15cm)で2個体のメダカを用いて視運動反応を同時に誘導すると初期状態によらず個体間距離が常に一定(2〜4cm)に保って遊泳することを発見した。また小型淡水フグ(体長3cm)でも同様の結果が得られ、本実験系は様々な魚類で用いることができることが分かった。また淡水フグとメダカの間では協調的行動が生じなかったことから、この協調的行動は同種個体間認識を介していることも示された(論文準備中)。本研究では申請者が独自に開発した実験系と分子遺伝学が駆使できるメダカを用いる事で魚類の協調的行動の脳の分子及び神経基盤を世界に先駆けて解明できることが期待される

  23. ミツバチの脳のキノコ体に特異的に発現するM5遺伝子の解析

    竹内 秀明

    1998年 ~ 1999年

︎全件表示 ︎最初の5件までを表示